LOCUS       DBJYSH010000001        73927 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.001, whole genome shotgun sequence.
ACCESSION   DBJYSH010000001 DBJYSH010000000
VERSION     DBJYSH010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 73927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 73927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..73927
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.001"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            <1..542
                     /locus_tag="ACUBV3_000001"
     CDS             <1..542
                     /locus_tag="ACUBV3_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HHU3846545.1"
                     /translation="SIIIGLMAAMFIIRLAYLKLSIANEKALRKNGAKEYGVGVSKAI
                     TVLHIIIYFSSVTEAILTKASFNFVSVIGLSLMIFSVFMLHTVTRLLGRIWTVKLMVD
                     KNHQFVDHWLFRVVKHPNYFLNIAPELLGVTLLCHAKYTALFVLPIYAFVIYLRIREE
                     NLLLKTIIIPNGIKKSRVS"
     gene            855..1769
                     /gene="glyQ"
                     /locus_tag="ACUBV3_000002"
     CDS             855..1769
                     /gene="glyQ"
                     /locus_tag="ACUBV3_000002"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HHU3846546.1"
                     /translation="MSKKLTFQEIILTLQQFWNDQGCMLMQAYDNEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPSNVQELYLKSLEL
                     LGINPLEHDIRFVEDNWENPSTGSAGLGWEVWLDGMEITQFTYFQQVGGLQTGPVTSE
                     VTYGLERLASYIQEVDSVYDIEWAPGVKYGEIFTQPEYEHSKYSFEISDQVMLLENFE
                     KFEREAKRALEEGLVHPAYDYVLKCSHTFNLLDARGAVSVTERAGYIARIRNLARVVA
                     KTFVAERKKLGFPLLDEETRIKLLAEED"
     gene            1769..2410
                     /locus_tag="ACUBV3_000003"
     CDS             1769..2410
                     /locus_tag="ACUBV3_000003"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001094117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0050446 - azobenzene reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HHU3846547.1"
                     /translation="MNTLIVNSHPDFSNPYSFTTILQEKFIELYNEHFPNHQLSILNL
                     YDCVLPEITKEVLLSIWSKQRKDLELTADEIVQAKISKDLLEQFKSHHRIVFVSPMHN
                     YNVTARAKTYIDNIFIAGETFKYTENGSVGLMTDDYRLLMLESAGSIYSKGQYSPYEF
                     PVHYLKAIFKDFMAFDDFCVVRAEGTDILDRQVVLDKMNQDLREAFEAFYSKE"
     gene            2414..4453
                     /gene="glyS"
                     /locus_tag="ACUBV3_000004"
     CDS             2414..4453
                     /gene="glyS"
                     /locus_tag="ACUBV3_000004"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HHU3846548.1"
                     /translation="MTKDLLLELGLEELPAYVVTPSEKQLGQKMVKFLEDHRLSFETV
                     QTFSTPRRLAVRVKGLADQQTDLTEDFKGPSKKIALDAEGNFSKAAQGFVRGKGLSVD
                     DIEFREVKGEEYVYVTKHETGKSAIDVLASVTEVLTELTFPVNMHWANNSFEYIRPVH
                     TLVVLLDDQALELDFLDIHSGRISRGHRFLGSDTEILSASSYEDDLRQQFVIADAKER
                     QQMIVDQIHAIEEKENISVEIDEDLLNEVLNLVEYPTAFLGSFDEKYLDVPEEVLVTS
                     MKNHQRYFVVRDRDGKLLPNFISVRNGNAEHIENVIKGNEKVLVARLEDGEFFWQEDQ
                     KLNIADLVEKLKQVTFHEKIGSLYEHMDRVKVISQYLAEKADLSDEEKLAVLRAASIY
                     KFDLLTGMVDEFDELQGIMGEKYALLAGEQPAVAAAIREHYMPTSADGELPETRVGAI
                     LALADKFDTLLSFFSVGLIPSGSNDPYALRRATQGIVRILEAFGWDIPLDELVTNLYG
                     LSFASLDYANQKEVMAFISARIEKMIGSKVPKDIREAVLESDTYIVSLILEASQALVQ
                     KSKDAQYKVSVESLSRAFNLAEKVTHSVSVDYSLFENNQEKALYQAILSLELTEDMHD
                     NLDKLFALSPIINDFFDNTMVMTDDEKMKQNRLALLNSLVAKARTVAAFNLLNTK"
     gene            4465..4722
                     /locus_tag="ACUBV3_000005"
     CDS             4465..4722
                     /locus_tag="ACUBV3_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HHU3846549.1"
                     /translation="MDPKKIARINELSKKKKTVGLTGEEKVEQAKLREEYIEGFRRSV
                     RHHVEGIKLVDDEGNDVTPEKLRQVQREKGLHGRSLDDPNS"
     gene            4811..5074
                     /locus_tag="ACUBV3_000006"
     CDS             4811..5074
                     /locus_tag="ACUBV3_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HHU3846550.1"
                     /translation="MIELLLALLLWNILVFATYAIDKRRAIRGAWRISEKTLLIESLL
                     LGGFGAFLGGEVFRHKTTKWYFRLVWYIGMIIDLAIIWLIWCY"
     gene            5188..6696
                     /gene="glpK"
                     /locus_tag="ACUBV3_000007"
     CDS             5188..6696
                     /gene="glpK"
                     /locus_tag="ACUBV3_000007"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000093523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HHU3846551.1"
                     /translation="MSSEEKYIMAIDQGTTSSRAIIFNKKGEKIASSQKEFPQIFPQA
                     GWVEHNANQIWNSVQSVIAGAFIESSIKPGQIEAIGITNQRETTVVWDKKTGLPIYNA
                     IVWQSRQTAPIADQLKQEGHTNMIHEKTGLVIDAYFSATKVRWILDHVPGAQERAEKG
                     ELLFGTIDTWLVWKLTDGLVHVTDYSNAARTMLYNIKELKWDDEILELLNIPKAMLPE
                     VKSNSEVYGKTTPFHFYGGEVPISGMAGDQQAALFGQLAFEPGMVKNTYGTGSFIIMN
                     TGEEMQLSQNNLLTTIGYGINGKVHYALEGSIFIAGSAIQWLRDGLRMIETSSESEGL
                     AQSSTSDDEVYVVPAFTGLGAPYWDSNARGSVFGLTRGTSKEDFVKATLQSIAYQVRD
                     VIDTMQVDSGIDIQQLRVDGGAAMNNLLMQFQADILGIDIARAKNLETTALGAAFLAG
                     LSVGYWESMDELKELNATGQLFQATMNESRKEKLYKGWRKAVKATQVFAQED"
     gene            6709..8538
                     /gene="glpO"
                     /locus_tag="ACUBV3_000008"
     CDS             6709..8538
                     /gene="glpO"
                     /locus_tag="ACUBV3_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HHU3846552.1"
                     /translation="MEFSRETRRLALQRMQDRTLDLLIIGGGITGAGVALQAAASGLD
                     TGLIEMQDFAEGTSSRSTKLVHGGLRYLKQFDVEVVSDTVSERAVVQQIAPHIPKPDP
                     MLLPVYDEPGSTFSMFRLKVAMDLYDLLAGVTNTPAANKVLSAEDVLKREPDLQKEGL
                     LGGGIYLDFRNNDARLVIENIKRANRDGAYIASHVKAEDFLFDDNNQIIGVRARDLLT
                     DQVIDIKARLVINTTGPWSDTVRNFSNEGKQIHQLRPTKGVHLVVDRQKLNVSQPVYV
                     DTGLNDGRMIFVLPREDKTYFGTTDTDYHGDLEHPTVTKEDVDYLLNIVNKRFPEAEL
                     TIDDIESSWAGLRPLLSGNSASDYNGGNSGKLSDESFEELIDSVKDYIAHKNHREDVE
                     KAISHVESSTSEKELDPSAVSRGSSFERDDNGLLTLAGGKITDYRKMAEGAMETIINI
                     LDKEYNRKFKLINSKTYPVSGGEINPSNVDSEIEAYAQLGTLSGLSIEDARYIANLYG
                     SNAPKLFALTRQITEAEGLSLVETLSLHYAMDYEMALSPTDFFLRRTNHMLFMRDNLD
                     SLIQPVIDEMAKHYQWSDQDKTFYEEELHETLKDNDLAALKDN"
     gene            8550..9248
                     /locus_tag="ACUBV3_000009"
     CDS             8550..9248
                     /locus_tag="ACUBV3_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HHU3846553.1"
                     /translation="MDIFGEFLGTALLVLLGNGVVAGVVLPKTKNHNSGWIVITFGWG
                     LAVAIAVLVSGNISPAHLNPAVSLAFAIKGDLAWGTAILYMIAQIIGAMLGSLLVYLQ
                     FRPHYEAAENKADILGTFATGPALKDNFSNFLSEVLGTLVLVLTIFAIGKYNMPPGVG
                     TMSVGMLVVGIGLSLGGTTGYAINPARDFGPRLLHALLPMKNKGDSDWTYSWIPIVGP
                     MVGAILAALIFAMM"
     gene            9336..10673
                     /locus_tag="ACUBV3_000010"
     CDS             9336..10673
                     /locus_tag="ACUBV3_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HHU3846554.1"
                     /translation="MTEKYVNKVIVILGASFAGMTCAQKLRQLNPNWDIVLIDKEIHP
                     DYVPNGLNWYYRHEISGLNQAMWQTEEEQRLQNIRCLFGLKVEKINKEDRELMLSDGS
                     SVYYDQLICAMGSQAESTYIDGADAQGVLTTKTYATSQNAKQVLDKSHKVAVVGAGII
                     GLDIAYSLHESGKAVTLLEAQERPDFRHTDPDMSLPLLDAMAESKLHFFQNQKVEKIT
                     VTREEKLCLRTLTGDTFTVDAVILAVNFRPDSRLLTGLVDLSVDNSVVVNDYFQTSDP
                     NIYAIGDLIWSYFKGLNSAYYMPLINQAIRSAQMLAYHLSGHAVPKLKITRATGSKHF
                     GYYRANIGLTELEAGLYEDTVSVTYFPKEQYDLRIKLIANQKTGHLLGAQLISKENCL
                     ATANQLVQAISCDMTDFDLAFQDFIYTARESEMAYMLHQAAINLYEKRIGLCE"
     gene            10664..12094
                     /locus_tag="ACUBV3_000011"
     CDS             10664..12094
                     /locus_tag="ACUBV3_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001217288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HHU3846555.1"
                     /translation="MRIEALMEKERRVQYRLLSFLRGSPQAIALKLALLETGLSRATL
                     LKYINNLNSYFEQEKVNCRIVYYKDKLFLEEDYNLSNQEVLKALMKDSIKYTILISLF
                     NQRQFTIVGLSQELMVSEATLNRHLAHLNELLAEFDIAISQGKQIGDELQWRYFYYEL
                     FKQLWSYDKCQNMIKKLDLDSLILLIERLAQHTLTREAQQNLGLWFSICHHRLLAMEK
                     ISDNLKPIVEHYQCNAFYKRLDAALVLYMSRFALEYREGEVLATFAFLHSQNILPINT
                     MEYIMGFGGPIIDCVTETIIYFKKESILADETSDQVIYQLGQLYSHYYFFKGHILVEQ
                     PDLEQTYRLIDHNMRDKLHHISKKIIANVNRIRPLTEGGCSLLTLHLLELLIFSKNSQ
                     KMPFRIGLDMTGNAVEQSLLEYRIRQHFSGNNSIQVEPYDEGKGFDMVIYQSRSRPYK
                     AKLTYCLNKGASERELQEIDSLIYDN"
     gene            12219..14204
                     /gene="tkt"
                     /locus_tag="ACUBV3_000012"
     CDS             12219..14204
                     /gene="tkt"
                     /locus_tag="ACUBV3_000012"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HHU3846556.1"
                     /translation="MTFDTIDQLAVNTVRTLSIDAIQAANSGHPGLPMGAAPMAYVLW
                     NKFLNVNPKTSRNWTNRDRFVLSAGHGSALLYSLLHLAGYDLSIDDLKQFRQWGSKTP
                     GHPEVNHTDGVEATTGPLGQGIANAVGMAMAEAHLAAKFNKPGFDLVDHYTYTLHGDG
                     CLMEGVSQEAASLAGHLKLGKLVLLYDSNDISLDGPTSQSFTEDVKGRFESYGWQHIL
                     VKDGNDLEAIAAAIEAAKAETDKPTIIEVKTIIGFGAEKQGTSSVHGAPLGAEGITFA
                     KKAYGWEYPDFTVPAEVVARFASDLQARGAKAEEAWNDLFAKYEVEYPELAAEYKEAF
                     AGQAETVELKAHDLGSSVASRVSSQQAIQQLSTQLPNLWGGSADLSASNNTMVAAETD
                     FQASNYAGRNIWFGVREFAMAAAMNGIALHGGTRVYGGTFFVFSNYLLPAVRMAALQN
                     LPTVYVMTHDSIAVGEDGPTHEPIEQLASVRSMPNLNVIRPADGNETNAAWQRAVSET
                     DRPTMLVLTRQNLPVLEGTSELAQEGVNKGAYILSEAKGELDGIIIATGSEVKLALDT
                     QDKLESEGIHVRVVSMPAQNIFDEQEASYQEQVLPSAVTKRLAIEAGSSFGWGKYVGL
                     NGLTLTIDTWGASAPGNRIFEEYGFTVENAVSLYKEL"
     gene            14417..14722
                     /locus_tag="ACUBV3_000013"
     CDS             14417..14722
                     /locus_tag="ACUBV3_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HHU3846557.1"
                     /translation="MKKECRDFYRQIQHTYNDIAVREDAVLSSILLSASNGLIKTSDV
                     PRVAYELTQQLENNEIEKSFESLATVKELKKSAKKLSERYEQNKVTTGLLSKVFGNH"
     gene            14742..15476
                     /locus_tag="ACUBV3_000014"
     CDS             14742..15476
                     /locus_tag="ACUBV3_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HHU3846558.1"
                     /translation="MIKFEHVSKVYGEKEALSDLTLSIKDGEIFGLIGHNGAGKTTTI
                     SILTSIIDATYGQVYIDDLLLTEHRDQIKKKIGYVPDSPDIFLNLTAEEYWYFLAKIY
                     DVAPEDIEARITKLVDIFELEEQRYNPIESFSHGMRQKVIVIGALLPNPDIWILDEPL
                     TGLDPQASFDLKEMMKEHAKNGKTVIFSTHVLAVAEQLCDRIGILKQGKLIFVGSLGE
                     LKMQYPDKDLETIYLELAGRQANREG"
     gene            15480..17084
                     /locus_tag="ACUBV3_000015"
     CDS             15480..17084
                     /locus_tag="ACUBV3_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HHU3846559.1"
                     /translation="MNWSRIWELVKINILYSNPQTLSALRKKQEKHPKKEFSAYKSMF
                     RNQLFQILLFSIIYVFLFVSLDFKEYPGYFTFYIGIFTLVSIIYSFIAMYSVFYESDD
                     VKQYAYLPIKSEELYVAKIFATFGMSVTFLMPILTLMIVAYWRIIGGPLAVLLAIINF
                     AILFLSVTVISLYINSLIGRAIIRSANRKLISTILISLATFGAIVPLLFVNMTSQKMV
                     QGKLQDIAPIPYVRGYYDIVTAPFSMESLLNYYLPLLIILFLIGAIYKWVMPRYYQEL
                     LYGQVKQRKVHRQIDFSKRESINKTLVKHHLSSLQNATLLTNTFLMPLLYLAMFIVPI
                     LNNGKEIGRFFNENYFGIAFLAGILIGSLCVMPASIVGVGISLEKSNFYFIKSLPISF
                     SYFLKHKFVTLITLQLAVPTFIYFLVGFFLLKLSILVLLSFILGLVFMGLIEGQFIYR
                     RDYKHLFLNWQEVTQLFNRGVGQWLLVGSLFGMMIIGSFLIGISIFWSMVWNTVAVNI
                     IILITGLLILSICQYLLLKNFWKKLV"
     gene            complement(17292..18677)
                     /locus_tag="ACUBV3_000016"
     CDS             complement(17292..18677)
                     /locus_tag="ACUBV3_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HHU3846560.1"
                     /translation="MSREYIALSKNIIKHLGGQNNINNVYHCQTRLRFSLNDPTKVNL
                     EQLKTLKEVKTVVISGGQHQIVIGTHVAKVFEEINSLIETNSTTKTEQTKKAKAVSRI
                     IDFVSGTFQPILPALSGAGMIKALLALLLVFKILTPLSQTYILLNLFADGVFYFLPIL
                     IAITAAQKLKANPILALGTVVMLLHPNWANLVASGKPVSLFHTIPFTLTNYASSVIPI
                     ILIICVQAYIEKYLNQIIPKSLRLVLVPMLIFLSMGILSFSILGPMGTIAGQYLAVIF
                     TFLSKYASWAPAFLVGAFAPILIMFGVHSGIAALGITQLTKLGFDSIFGPGMLCSNIA
                     QATAGTVVTLITKEKKLKEIAGPAAITAYMGITEPILYGVNLPKRYPLIASLIGGGLG
                     GLYAGIMNAHRFAVGSSGLPGLFLYISHTSTHLFITMLIAVIITVSTTAILTFILAQY
                     YEKQVSIRQRD"
     gene            18818..19621
                     /gene="proB"
                     /locus_tag="ACUBV3_000017"
     CDS             18818..19621
                     /gene="proB"
                     /locus_tag="ACUBV3_000017"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HHU3846561.1"
                     /translation="MKRHFETTRRIVIKVGTSSLVQTSGKINLSKIDHLAFVISSLMN
                     RGMEVILVSSGAMGFGLDILKMDKRPQEISQQQAVSSVGQVAMMSLYSQIFSHYQTHV
                     SQILLTRDVVVFPESLQNVTNSFESLLSMGILPIVNENDAVSVDEMDHKTKFGDNDRL
                     SAVVAKITKADLLIMLSDIDGLFDKNPNIYDDAVLRSHVSEITDDIIKSAGGAGSKFG
                     TGGMLSKIKSAQMVFDNNGQMILMNGANPRDILKVLDGHNIGTYFAQGK"
     gene            19631..20884
                     /locus_tag="ACUBV3_000018"
     CDS             19631..20884
                     /locus_tag="ACUBV3_000018"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HHU3846562.1"
                     /translation="MTYIEILGQNAKKASQSVARLSTASKNEILRDLARNIVADTETI
                     LTENARDVAKAKDNGISEIMVDRLRLNKDRIQAIANGIYQVADLADPIGQVVSGYTNL
                     DGLKILKKRVPLGVIAMIFESRPNVSVDAFSLAFKTGNAIILRGGKDAIFSNTALVNC
                     MRQTLQDTGHNPDIVQLVEDTSHVVAEELMQATDYVDVLIPRGGAKLIQTVKEKSKIP
                     VIETGVGNVHIYIDEFADLDMAAKIVINAKTQRPSVCNAAEGLVVHQAIAKGFLSQLE
                     KMLKESNQSVEFRADEEALQLLENAVAASESDYATEFLDYIMSVKVVDSFEQAISWIN
                     KYSSHHSEAIITNNISRAEIFQDMVDAAAVYVNASTRFTDGFVFGLGAEIGISTQKLH
                     ARGPMGLEALTSTKYYINGTGQVRE"
     gene            20967..21914
                     /gene="rsmH"
                     /locus_tag="ACUBV3_000019"
     CDS             20967..21914
                     /gene="rsmH"
                     /locus_tag="ACUBV3_000019"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HHU3846563.1"
                     /translation="MTNDFHHITVLLHETVDMLDIKPDGIYVDATLGGAGHSEYLLSQ
                     LGPDGHLYAFDQDQKAIDNAHIRLKKYVDTGQVTFIKDNFRNLSSNLKALGVSEINGI
                     CYDLGVSSPQLDERERGFSYKQDAPLDMRMNREQSLTAYDVVNTYSYHDLVRIFFKYG
                     EDKFSKQIARKIEQVRAEKPISTTTELAEIIKSSKSAKELKKKGHPAKQIFQAIRIEV
                     NDELGAADESIQQAMDLLAVDGRISVITFHSLEDRLTKQLFKEASTVEVPKGLPFIPD
                     DLQPKMELVNRKPILPSQEELEANNRAHSAKLRVARRIR"
     gene            21929..22255
                     /gene="ftsL"
                     /locus_tag="ACUBV3_000020"
     CDS             21929..22255
                     /gene="ftsL"
                     /locus_tag="ACUBV3_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HHU3846564.1"
                     /translation="MTNEKRTEAVTQTLQRHIKTFSRIEKAFYGAIVITAIIMAVGII
                     YLQSNSLQVKQEVNQLNSKINDKQTEFDNAKQEVNELSNRDRITKIAKDAGLTIQNDN
                     IYRKVD"
     gene            22259..24517
                     /gene="pbp2X"
                     /locus_tag="ACUBV3_000021"
     CDS             22259..24517
                     /gene="pbp2X"
                     /locus_tag="ACUBV3_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein PBP2X"
                     /protein_id="HHU3846565.1"
                     /translation="MTFFKKLKKIFLDYVIHIRDRRSPQKNRERVGQNLMILTIFLFF
                     IFIINFVIIVGTDSKFGVNLSKEAKKVYQQSMTVQAKRGTIYDRNGNPIAEDATTYSL
                     YAIISKNYTTATGQKLYVQPSQYEKVASILENKLGMKKNLVLKQLNQKKLFQVSFGSS
                     GSGLSYTKMADIKKTMEKSDIKGIGFSTSPGRIYPNGIFASQFIGFTLPQDDGDGKKL
                     VGNTGLEAALNKVLSGTDGKVTYEKDRSGNVLLGTATTERRAVNGKDIYTTLSEPIQT
                     VLETQMDVFAEKTKGKFASATVVNAKTGEILATSQRPTYNPSTLKGYDKKNLGTYNTL
                     LYDNFFEPGSTMKVMTLASAIDSKHFNSTEVYNSAQYKIADAVIRDWDVNEGLSSGSY
                     MTFPQGFAHSSNVGMVTLEQKMGRDKWLNYLSKFKFGYPTRFGMLHESGGLFPSDNEV
                     TIAMSSFGQGIGVTQVQMLRAFTSISNDGVMLQPQFISSIYDPNTGTSRTARKEVVGK
                     PISKEAASKTRDYMVTVGTDPYYGTLYAAGAPVIQVGNQSVAVKSGTAQIAQEGGGGY
                     LQGKNDTINSVVAMVPSENPDFIMYVTIQQPEKFSITFWKDVVNPVLEQATAMKETIL
                     KPGLNDSEHQTKYKLSKIVGENPGHVAEELRRNLVQPIILGNGSKVSKVSKRPGANLA
                     ENEQLLVLTNKLTELPDMYGWSKANVEQFAKWTGIKVTYKGSTSGKVRKQSIDVGKSI
                     NKIKKIKITIGD"
     gene            24519..25529
                     /gene="mraY"
                     /locus_tag="ACUBV3_000022"
     CDS             24519..25529
                     /gene="mraY"
                     /locus_tag="ACUBV3_000022"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HHU3846566.1"
                     /translation="MFLSIMAGVIAFVLTVIAIPRFIKFYQLKKIGGQQMHEDVKQHL
                     AKAGTPTMGGTVFLIVALLVSLIFSIILSKENSGNLGATFGILSVVLIYGIIGFLDDF
                     LKIFKQINEGLTPKQKMSLQLIAGLIFYFVHVLPSGTSAINIFGFNLEVGYLYAFFVL
                     FWVVGFSNAVNLTDGIDGLASISVVISLITYGIIAYNQTQFDILLIIVIMIGALLGFF
                     VFNHKPAKVFMGDVGSLALGAMLAAISIALRQEWTLLFIGFVYVFETSSVMLQVAYFK
                     YTKKKTGVGKRIFRMTPFHHHLELGGVSGKGNKWSEWKVDAFLWAIGIFMSAITLAIL
                     YL"
     gene            25627..26970
                     /locus_tag="ACUBV3_000023"
     CDS             25627..26970
                     /locus_tag="ACUBV3_000023"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HHU3846567.1"
                     /translation="MSFKDFNFKPYIQRALDELKFVDPTDVQAKLIPVVRSGRDLVGE
                     SKTGSGKTHTFLLPIFEKLDESSNDVQVVITAPSRELATQIYQATKQIAEHSEQEIRV
                     VNYVGGTDKLRQIEKLKVSQPHIVIGTPGRIYDLVKSGDLAIHKAHTFVVDEADMTLD
                     MGFLDTVDKIAGSLPKDVQILVFSATIPQKLQPFLKKYLTNPVMEKIKTATVITDTID
                     NWLLSTKGRDKNAQILELSKLMQPYLAMIFVNTKERADELHSYLSSNGLKVAKIHGGI
                     APRERKRIMNQVKNLEFEYIVATDLAARGIDIEGVSHVINDAIPQDLSFFVHRVGRTG
                     RNGLSGTAITLYQPSDDSDIRELEKLGINFIPKVIKNGEFQDTYDRDRRNNREKSYQK
                     LDTEMIGLVKKKKKKIKPGYKKKIQWKVDEKRRKERRASNRAKGRAERKAKKQSF"
     gene            27108..27920
                     /locus_tag="ACUBV3_000024"
     CDS             27108..27920
                     /locus_tag="ACUBV3_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HHU3846568.1"
                     /translation="MFKKIILSIATIVATASLAVSVQASEKVELKVATDSDTAPFTYQ
                     KDGKFKGYDVDVVKAVFKGSKYKVTFKTVPFDTISTGIDAGKFDLSANDFSYNKERAE
                     KYLFSEPISRSNYAVVGKKGSHYKSLSDLSGKSTEVLSGVNYAQVLENWNKNHPNKKP
                     IKIKYVSGTTGVTSRLKNIESGKIDFILYDAISSDYIVKDQSLNLSVSPLKGKIGNNK
                     DGLEYLLLPKDKKGKTLQKFINKRIKVLKEDGTLARLSKQYFGGDYVSNIDK"
     gene            28015..28818
                     /locus_tag="ACUBV3_000025"
     CDS             28015..28818
                     /locus_tag="ACUBV3_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HHU3846569.1"
                     /translation="MNQFILTGGWSWYNNLVSQVPAGKLFSWKAVLDAIPSILERLPI
                     TLLLTVAGALFGLILALIFAIVKINRVKILYPIQALFVSFLRGTPILVQLMLSYYGIP
                     LFLKFLNQKYGFDWNINAIPASVFAITAFAFNEAAYTSETIRAAILSVDQGEIEAARS
                     LGMTSAQVYRRVIIPNAAVVATPTLINTLIGLTKGTSLAFNAGIVEMFAQAQIMGGSD
                     YRYFERYISVALVYWAVSFLIEQLGNAIERKMAIKAPRHLTDEIPGGVR"
     gene            28818..29561
                     /locus_tag="ACUBV3_000026"
     CDS             28818..29561
                     /locus_tag="ACUBV3_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HHU3846570.1"
                     /translation="MIKLRQLTKSFSGQKVLDKLDLDIEKGQVVALVGASGAGKSTFL
                     RSMNYLEEPDYGTIEIDDFKVDFKSISKDDILTLRRKLAMVFQQFNLFERRTALDNVK
                     EGLKIVKKMSDQEATRIARDELAKVGLADREKYYPRHLSGGQKQRVALARALAMKPDV
                     LLLDEPTSALDPELVGEVEKSIADAAKQGQTMVLVSHDMNFVYQVADKVLFLEKGRIL
                     ESGTPEQLFNHPLEERTKEFFASYNKSYL"
     gene            29683..29907
                     /locus_tag="ACUBV3_000027"
     CDS             29683..29907
                     /locus_tag="ACUBV3_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4059 family protein"
                     /protein_id="HHU3846571.1"
                     /translation="MFLEIAKVYLQGLGLASITVILVGIIWVFWRAKKNIDKTARERQ
                     SVLYDVLLIALMTAPILAFAFVAIILMIKA"
     gene            29976..30890
                     /gene="trxB"
                     /locus_tag="ACUBV3_000028"
     CDS             29976..30890
                     /gene="trxB"
                     /locus_tag="ACUBV3_000028"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HHU3846572.1"
                     /translation="MYDTLIIGSGPGGMTAALYAARSNLKVGLIEQGAPGGQMNNTAE
                     IENYPGYDHISGPELSMKMYEPLEKFEVEHIYGIVQRVENDGDVKRVITEDESYEAKT
                     VILATGAKNSLLGVPGEEEYTSRGVSYCAVCDGAFFRDQDLLVVGGGDSAVEEAVFLT
                     QFAKSVTIIHRRDQLRAQKVLQDRAFANEKIKFVWDSVVKEIKGNEIKVSGVTVENLK
                     TGEISEMPFGGVFIYVGLKPHSSMVSELGITDETGWVLTDTNMKTSIPGLYAIGDVRQ
                     KDLRQIATAVGEGAIAGQGVYNYITEHF"
     gene            31046..32506
                     /locus_tag="ACUBV3_000029"
     CDS             31046..32506
                     /locus_tag="ACUBV3_000029"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HHU3846573.1"
                     /translation="MYKDDSLTLHTDLYQINMMQVYFNKGIHNKKAVFEAYFRKVPFE
                     NGYAVFAGLERIVRYLENLSFSDSDLSYLEELGYPEEFLDYLKNLKMELTVKSAKEGD
                     LVFANEPLVQIEGPLAQCQLVETAILNIINYQTLVATKAARIRSVIEDEPLLEFGTRR
                     AQEMDAAIWGTRAAIIGGANATSNVRAGKIFNIPVSGTHAHALVQTYGDDYQAFKAYA
                     ETHKDCVFLVDTYDTLRVGVPNAIRVAKEMGEKINFLGVRLDSGDLAYLSKKVRQQLD
                     DAGFPNAKIYASNDLDENTILNLKMQKAKIDVWGVGTKLITAYDQPALGAVYKIVSIE
                     TDAGSMRDTIKLSNNAEKVSTPGKKQVWRITSRAKGKSEGDYITFADTDVTQLDEIDM
                     FHPTYTYINKTVRDFDAVPLLVDIFDKGKLVYQLPSLQEIQEYGRKEFDQLWDEYKRV
                     LNPQDYPVDLARDVWQNKMDLIDRIRKEALAKGEVR"
     gene            32503..33324
                     /gene="nadE"
                     /locus_tag="ACUBV3_000030"
     CDS             32503..33324
                     /gene="nadE"
                     /locus_tag="ACUBV3_000030"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002949400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HHU3846574.1"
                     /translation="MTLQDQIIKELGVKPVINPSQEIRRSVEFLKDYLLKHSFLKTYV
                     LGISGGQDSTLAGRLAQLAVEELRADTGENYQFIAIRLPYGIQADEEDAQKALDFIKP
                     DIALTINIKEAVDGQVRALNAAGVEITDFNKGNIKARQRMISQYAVAGQYAGAVIGTD
                     HAAENITGFFTKFGDGGADLLPLFRLNKSQGKQLLAELGADKALYEKIPTADLEENKP
                     GIADEIALGVTYQEIDAYLEGKVVSDKSRDIIENWWYKGQHKRHLPITIFDDFWK"
     gene            33437..34771
                     /gene="pepC"
                     /locus_tag="ACUBV3_000031"
     CDS             33437..34771
                     /gene="pepC"
                     /locus_tag="ACUBV3_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase C"
                     /protein_id="HHU3846575.1"
                     /translation="MSELTQTFTDKLFADYQANTKFSAIENAVTHNGLLKSLETRQSE
                     IENDYVFSIDLTKDEVSNQKQSGRCWMFAALNTFRHKLISDFKLENFELSQAHTFFWD
                     KYEKSNWFMEQIIATANQELSSRKVKFLLDVPQQDGGQWDMVVALFEKYGVVPKTVYP
                     ESVSSSASRELNQYLNKLLRQDAQILRELIAQGADGATVQNKKEELLQEIFNFLAMNL
                     GLPPQSFDFAYRDKDNHYQSDKNITPKAFYQKYVNLDLSDYVSIINAPTVDKPYGQSY
                     TVEMLGNVVGGPAVKYLNLDMKRFKELAIAQMKSGETVWFGSDVGQVSNRQKGILATT
                     TYDFNSSMDIKLSQDKAGRLDYSESLMTHAMVLTGVDLDESGQPLKWKVENSWGEKVG
                     KDGYFVASDAWMDEYTYQIVVRKELLTKEELEAYNAEPITLAPWDPMGALAN"
     gene            complement(34818..37058)
                     /gene="pbp1a"
                     /locus_tag="ACUBV3_000032"
     CDS             complement(34818..37058)
                     /gene="pbp1a"
                     /locus_tag="ACUBV3_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein PBP1A"
                     /protein_id="HHU3846576.1"
                     /translation="MITIKKESVIKLLKYAFGIIMGFIILTIVIGGLLFAYYVSRSPK
                     LTDQALKSVNSSLVYDGNNKLIADLGSEKRESVSADSIPLNLVNAITSIEDKRFFKHR
                     GVDIYRILGAAWHNLVSSNTQGGSTLDQQLIKLAYFSTNKSDQTLKRKSQEVWLALQM
                     ERKYTKEEILTFYINKVYMGNGNYGMRTTAKSYFGKDLKELSIAQLALLAGIPQAPTQ
                     YDPYKNPESAQTRRNTVLQQMYQDKNISKKEYDQAVATPVTDGLKELKQKSTYPKYMD
                     NYLKQVISEVKQKTGKDIFTAGLKVYTNINTDAQKQLYDIYNSDTYIAYPNNELQIAS
                     TIMDATNGKVIAQLGGRHQNENISFGTNQSVLTDRDWGSTMKPISAYAPAIDSGVYNS
                     TGQSLNDSVYYWPGTSTQLYDWDRQYMGWMSMQTAIQQSRNVPAVRALEAAGLDEAKS
                     FLEKLGIYYPEMNYSNAISSNNSSSDAKYGASSEKMAAAYSAFANGGTYYKPQYVNKI
                     EFSDGTNDTYAASGSRAMKETTAYMMTDMLKTVLTFGTGTKAAIPGVAQAGKTGTSNY
                     TEDELAKIEATTGIYNSAVGTMAPDENFVGYTSKYTMAIWTGYKNRLTPLYGSQLDIA
                     TEVYRAMMSYLTGGYSADWTMPEGLYRSGSYLYINGTTTTGTYSSSVYKNIYQNSGQS
                     SQSSSSTSSEKQKEDKNTANDANSSSPQVETPNNGNATTPNNSNQTVPGTGHGNGNSN
                     TAPNGN"
     gene            complement(37045..37644)
                     /gene="recU"
                     /locus_tag="ACUBV3_000033"
     CDS             complement(37045..37644)
                     /gene="recU"
                     /locus_tag="ACUBV3_000033"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HHU3846577.1"
                     /translation="MVNYPHQLIRKTTVTKSKKKKIDFANRGMSFEAAINATNDYYLS
                     HELAVIHKKPTPVQIVKVDYPKRSRAKIVEAYFRQASTTDYSGVYKGYYIDFEAKETR
                     QKTAMPMKNFHAHQIEHMANVLQQKGICFVLLHFSTLKETYLLPAHELISFYQIDKGN
                     KSMPIDYIRKNGFFVKESAFPQVPYLDIIEEKLLGGDYN"
     gene            37719..38237
                     /locus_tag="ACUBV3_000034"
     CDS             37719..38237
                     /locus_tag="ACUBV3_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HHU3846578.1"
                     /translation="MKSTILVTGYKNFELGIFQDKDPRITIIKKAIDKDFRRFLENGA
                     DWFIFMGNLGFEYWALEVALDLQKEYDFQIATIFTFENHGQNWNEANKAKLALFKQVD
                     FVKYTFPSYENPGQFKQYNHFLINNTQGAYLFYDSENETNLKFLLEMMKKKEAYDISF
                     LTFDRLNEIYEE"
     gene            38358..38690
                     /gene="gpsB"
                     /locus_tag="ACUBV3_000035"
     CDS             38358..38690
                     /gene="gpsB"
                     /locus_tag="ACUBV3_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HHU3846579.1"
                     /translation="MASIIYSPKDIFEQDFKVSMRGYDKKEVDVFLDDVIKDYENYLE
                     QIEKLQMENRRLQQALDKKEREASNVRNSGTAMYNQKPIAQSATNFDILKRISRLEKE
                     VFGRQIRE"
     gene            38709..39077
                     /gene="rnpB"
                     /locus_tag="ACUBV3_000036"
     ncRNA           38709..39077
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACUBV3_000036"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            39160..40314
                     /locus_tag="ACUBV3_000037"
     CDS             39160..40314
                     /locus_tag="ACUBV3_000037"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing class I SAM-dependent
                     RNA methyltransferase"
                     /protein_id="HHU3846580.1"
                     /translation="MKESFKLIATAAAGLEAIVGREIRNLGIDCQVENGRVRFHGDIK
                     TIIETNLWLRAADRIKIIVGEFPARTFEELFQGVYGLDWENYLPLGAKFPIAKAKCVK
                     SKLHNEPSVQAISKKAVAKKLQKVFHRPEGVPLQENGAEFKIEVSILKDKATVMIDTT
                     GSSLFKRGYRAEKGGAPIKENMAAAIIQLSNWFPDKPLIDPTCGSGTFCIEAAMIGMN
                     IAPGFNRDFAFEAWPWVDQSQVQKVRDEAESKANYDIDLDISGFDLDGRMVEIARKNA
                     EEAGLGDVIKLKQMRLQDLKTDKINGVIISNPPYGERLLDDKAVDILYNEMGQTFAPL
                     KTWSKFILTSDEGFEKKYGSQADKKRKLYNGTLKVDLYQYYGERVRRQVK"
     gene            40327..41790
                     /locus_tag="ACUBV3_000038"
     CDS             40327..41790
                     /locus_tag="ACUBV3_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division site-positioning protein MapZ
                     family protein"
                     /protein_id="HHU3846581.1"
                     /translation="MSEDQKHPFFEPEKETDGLEFKDAKEMTVEEAVRKDSEIKAGIT
                     EEDSILDKYIKQHRDEVASQKFETKSSDFANLDTASLDDFIKKQREELSAMLAAEELS
                     KKLDNSVSQEQDTEANAVSPKEESSQEQENSVTPVPPLITEAEPTATEPDSTIADSEE
                     YKSSSRKRGGIVGTLIALILLLIVAIFGYNYFKNNNSTNSQTATSQSSSSKATTTSSE
                     EDKKASQNLDNFNKAYANFFVDDKKTQLKNSEFDKLSELEKKVDALKGTKYYGKVKVK
                     FDSLKRQIDAVKAVNDKFKSPAVVDGKKSEKLEVKDGANFDSLDSKTLNTGNASLDSL
                     LHSIVSTGRNQVKQSEEQASSNKVSDTQTTEQPNVTNGQSSSSAATINNQAAGTASGN
                     LERNRSRVPYNNAAIADTGNPAWIFNPGVLEKIVATSQARGYFSGNNYILEPVNIING
                     NGYYNMFKSDGTYLFSINAKTGYFVGNAPGRADSLDY"
     gene            complement(42015..42497)
                     /locus_tag="ACUBV3_000039"
     CDS             complement(42015..42497)
                     /locus_tag="ACUBV3_000039"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002885585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HHU3846582.1"
                     /translation="MTKEVVVESFELDHTIVKAPYVRLISEEVGPVGDIITNFDIRLI
                     QPNENAIDTAGLHTIEHLLAKLIRQRINGLIDCSPFGCRTGFHMIMWGKQDATEIAKV
                     IKSSLEAIAGGVTWEDVPGTTIESCGNYKDHSLHSAQEWAKLILSQGISDNAFERHIV
                     "
     gene            42686..44293
                     /locus_tag="ACUBV3_000040"
     CDS             42686..44293
                     /locus_tag="ACUBV3_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HHU3846583.1"
                     /translation="MFNIILAMVCALIGLIIGYVAISMKMKSSKEAAELTLLNAEQDA
                     VDLRGKAEIEAEHIRKAAERESKAHQKELLLEAKEEARKYREEIEKEFKSDRQELKQM
                     EARLTDRASSLDRKDENLSNKEKMLDSKEQSLTDKSRHINEREQEIATLETKKVEELS
                     RIAELSQEEAKDIILADTEKDLAHDIATRIKEAEREVKERSNKIAKDLLAQAMQRLAG
                     EYVTEQTITTVHLPDDNMKGRIIGREGRNIRTLESLTGIDVIIDDTPEVVVLSGFDPI
                     RREIARMTLESLIQDGRIHPARIEELVEKNRLEMDQRIREYGEAAAYEIGAPNLHPDL
                     IKIMGRLQFRTSYGQNVLRHSVEVGKLAGILAGELGENVDLARRAGFLHDMGKAIDRE
                     VEGSHVEIGMEFARKYKEHPIVVNTIASHHGDVEPDSVIAVIVAAADALSSARPGARN
                     ESMENYIKRLRDLEEIANGFEGVQNAFALQAGREIRIMVQPGKVSDDQVVIMSHKVRE
                     KIEQNLDYPGNIKVTVIREMRAVDFAK"
     gene            44422..44901
                     /locus_tag="ACUBV3_000041"
                     /pseudo
     CDS             44422..44901
                     /locus_tag="ACUBV3_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029972.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            44903..45625
                     /locus_tag="ACUBV3_000042"
                     /pseudo
     CDS             44903..45625
                     /locus_tag="ACUBV3_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000791351.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            45627..46217
                     /locus_tag="ACUBV3_000043"
                     /pseudo
     CDS             45627..46217
                     /locus_tag="ACUBV3_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502504.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            46267..46971
                     /locus_tag="ACUBV3_000044"
     CDS             46267..46971
                     /locus_tag="ACUBV3_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001168921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA];
                     GO_function: GO:0022841 - potassium ion leak channel
                     activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HHU3846584.1"
                     /translation="MQKFKERFDTLSDAILAIAMTILVLEIKTPATMGDIGDFTRNIG
                     LFIVSFVVVFNFWYERAQNSLDAQKTNDEIIALDIIEHLGICLIPLFTKFMISFENHN
                     FAVMAYGLLTLLVGLTSDIIRIHLASYDLVTIPSELKERVIKVMTTFAIRSVVVRFII
                     IILAYFLPEVGIFAYLVIPLFMFWRRSRAGKYYAKKGIEAPSYIKLFIQIGERVPEDF
                     TNSSSGIGGVKRLYKK"
     gene            47138..47767
                     /gene="gmk"
                     /locus_tag="ACUBV3_000045"
     CDS             47138..47767
                     /gene="gmk"
                     /locus_tag="ACUBV3_000045"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HHU3846585.1"
                     /translation="MSERGLLIVFSGPSGVGKGTVRQEIFSTPDHKFDYSVSMTTRPQ
                     RPGEVDGVDYFFRTREEFEALIKEGQMLEYAEYVGNYYGTPLSYVNETLDKGIDVFLE
                     IEVQGALQVKSKVPDGVFIFLTPPDLEELEERLVGRGTDSPEVIAQRIERAKEEIALM
                     REYDYAVVNDQVSLAAERVKRVIEAEHYRVDRVIGRYTNMVKETDKKLS"
     gene            47790..48104
                     /gene="rpoZ"
                     /locus_tag="ACUBV3_000046"
     CDS             47790..48104
                     /gene="rpoZ"
                     /locus_tag="ACUBV3_000046"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HHU3846586.1"
                     /translation="MMLKPSIDTLLDKVPSKYSLVILQAKRAHELEAGEKATQDFKSV
                     KSTLRALEEIESGNVVIHPDPSAKRASVRARIEAERLAKEEEERKIKEQIAKEKEDGE
                     KI"
     gene            48178..50568
                     /locus_tag="ACUBV3_000047"
     CDS             48178..50568
                     /locus_tag="ACUBV3_000047"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000101529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HHU3846587.1"
                     /translation="MARKLAQVIVDIPLMQTDKPFSYAIPKDLEDLVQVGARVHVPFG
                     RGNRLLQGFVVGFRDDDELETKDIAGVLDFEPVLNQEQLDLADQMRHTVFSYKISILK
                     SMLPSLLNSQYDKLLLATDTLPSEDREDLFGHKTEIVFSSLSSQDAKKAGRLIQKGFI
                     EVQYLAKDKKTIKTEKIYKINRTLLEKSQIAARAKKRLELKEFLLENPQPGRLTALNK
                     QFSSPVVNFFREEGIIEVIEKEASRSDNYFKGILKTDFLDLNQEQAKVVKIVVDRIGK
                     DQNKPFLLEGITGSGKTEVYLHIIDNVLKLGKTAIVLVPEISLTPQMTNRFISRFGKR
                     VAIMHSGLSDGEKFDEWRKIKSGQAKVVVGARSAIFAPLENIGAIIIDEEHESTYKQE
                     SNPRYHARDVALLRAEYHKAVLLMGSATPSIESRARASRDVYKFLELKHRANPKARIP
                     QVEIIDFRNFIGQQEVSNFTPYLLDKIRDRLDKKEQVVLMLNRRGYSSFIMCRDCGYV
                     DQCPNCDISLTLHMATKTMNCHYCGFEKPIPRTCPNCNSKSISYYGTGTQKAYEELLK
                     VIPDAKILRMDVDTTRQKGGHESILKRFGNHEADILLGTQMIAKGLDFPNVTLVGVLN
                     ADTSLNLPDFRSSERTFQLLTQVAGRAGRAEKEGEVVIQTYNPNHYAIQLAQKQDFEA
                     FYQYEMNIRRQLGYPPYYFTVGLTLSHKDEEWLIRKSYEVLSLLKQGFSDKVKLLGPT
                     PKPIARTHNLYHYQIIIKYRFEDNLELVLNRLLDMTQDKENRDLRLAIDHEPQNMM"
     gene            50615..51550
                     /gene="fmt"
                     /locus_tag="ACUBV3_000048"
     CDS             50615..51550
                     /gene="fmt"
                     /locus_tag="ACUBV3_000048"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HHU3846588.1"
                     /translation="MTKLLFMGTPDFSATVLKGILADGKYDVLAVVTQPDRAVGRKKE
                     IKMTPVKEVALENNIPVYQPEKLSGSPELEQLMTLGADGIVTAAFGQFLPTKLLESVG
                     FAINVHASLLPKYRGGAPIHYAIINGEKEAGVTIMEMVAKMDAGDMVSKASVEITDED
                     NVGTMFDRLAVVGRDLLLDTLPGYLSGDIKPIPQNEEEVSFSPNISPDEERIDWNKSS
                     RDIFNHVRGMYPWPVAHTLLEGNRFKLYEVTMSEGKGSPGQVIAKTKNSLTVATGDGA
                     IELKSVQPAGKPRMDIKDFLNGVGRNLEIGDKFGE"
     gene            51540..52862
                     /gene="rsmB"
                     /locus_tag="ACUBV3_000049"
     CDS             51540..52862
                     /gene="rsmB"
                     /locus_tag="ACUBV3_000049"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HHU3846589.1"
                     /translation="MANDWKKSARGLALMTLEEVFDKGAYSNIALNKSLKKSRLSDKD
                     RALVTEIVYGTVARKITLEWYLSHFIVDRDKLELWVYHLLLLSLYQLLYLDNIPDHAI
                     VNDAVTIAKNRGNKKGAEKLINAVLRRVSSETLPEIASIKRQNKRYSVAYSMPVWLVK
                     KLIDQYGETRALAIMESLFERNKASLRVTDLSQKQTIKETLNVRDSHIAETALVADSG
                     NFASTSFFQDGLITIQDESSQLVAPTLKVSGNDQVLDACSAPGGKTSHIASYLTTGAV
                     TALDLYDHKLELVMENAKRLGLSDKIKTKKLDASKAHEYFPEDTFDKILVDAPCSGIG
                     LIRRKPDIKYNKANQDFEALQEIQLSILSSVCQTLRKGGIITYSTCTIFEEENFQVIE
                     KFLANHPNFEQVELSHTQEDIVKRGCISISPEQYHTDGFFIGQVKRIL"
     gene            52900..53637
                     /locus_tag="ACUBV3_000050"
     CDS             52900..53637
                     /locus_tag="ACUBV3_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HHU3846590.1"
                     /translation="MEISLLTDIGQRRSNNQDFINQFENKAGVPLIILADGMGGHRAG
                     NIASEMTVTDLGSDWAETDFSELSEIRDWMLVSIETENRKIYELGQSDDYKGMGTTIE
                     AVAIVGDNIIFAHVGDSRIGIVRQGEYHLLTSDHSLVNELVKAGQLTEEEAASHPQKN
                     IITQSIGQANPVEPDLGVHLLEEGDYLVVNSDGLTNMLSNADIATVLTQEKNLDDKNQ
                     DLITLANHRGGLDNITVALVYVESEAV"
     gene            53637..55592
                     /gene="pknB"
                     /locus_tag="ACUBV3_000051"
     CDS             53637..55592
                     /gene="pknB"
                     /locus_tag="ACUBV3_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000614563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HHU3846591.1"
                     /translation="MIQIGKLFAGRYRILKSIGRGGMADVYLARDLILDNEEVAIKVL
                     RTNYQTDQIAVARFQREARAMAELTHPNIVAIRDIGEEDGQQFLVMEYVDGFDLKKYI
                     QDNAPLSNNEVVRIMNEVLSAMSLAHQKGIVHRDLKPQNILLTKKGTVKVTDFGIAVA
                     FAETSLTQTNSMLGSVHYLSPEQARGSKATVQSDIYAMGIMLFEMFTGHIPYDGDSAV
                     TIALQHFQKPLPSILAENKSVPQALENIVIKATAKKLTDRYKTTYEMGRDLSTALSST
                     RHREPKLVFNDTESTKTLPKVTSTVSSLTTEQLLRNQKQAKTTEKITPDSASNDKTKS
                     KKKASHRLLGTIMKLFFALCVVGIIVFAYKILVSPTTIRVPDVSNKTVAQAKMTLENS
                     GLKVGAIRNIESDSVSEGLVVKTDPAAGRSRREGAKVNLYIATPNKSFTLGNYKEHNY
                     KDILKDLQGKGVKKSLIKVRRKINNDYTTGTILAQSLPEGTSFNPDGNKKLTLTVAVN
                     DPMIMPDVTGMTVGEVIETLTDLGLDADNLVFYQMQNGVYQAVVTPPSSSKIASQDPY
                     YGGEVGLRRGDKVKLYLLGSKTTNNSSSTPIDSSASSSTGTTTSDSVSSSTDASTSDS
                     SSTSTSSSTLPSDSTTNTGTANNPLTQ"
     gene            55751..56446
                     /gene="liaF"
                     /locus_tag="ACUBV3_000052"
     CDS             55751..56446
                     /gene="liaF"
                     /locus_tag="ACUBV3_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein
                     LiaF"
                     /protein_id="HHU3846592.1"
                     /translation="MKKFQFFLLVEAVVLVMGLMKILSDDWTSFIFILALILLALRFY
                     NNDSRHNFLLTTSLLLLFLIFMLNPYIIAAVVFAVLYVLINHFSQVKKKNRYALIQFK
                     NHQLDVKTTRNQWLGTDQHESDFYAFEDINIIRISGTDTIDLTNVIVSGQDNVIIIQK
                     VFGDTKVLVPLDVAVKADISSVYGSVQYFDFEEYDLRNESIKLSQEEEYYLLKRVKLV
                     VNTIAGKVEVSRK"
     gene            56443..57462
                     /locus_tag="ACUBV3_000053"
     CDS             56443..57462
                     /locus_tag="ACUBV3_000053"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HHU3846593.1"
                     /translation="MKKHHYFLAFFYGSVIIFAICFVIIDSLGVNLVHLYQTSRLWLI
                     EQLIFSIFFLSLAVTILLLLTWFLLDDNSKRQINHNLRRILNNQSINVTDDGTEISTN
                     IQRLSKKMNLMTASLQSKENSRILKSQEIVKQERKRIARDLHDTVSQDLFAASMVLSG
                     IAQNVSQLDVDQVGSQLLAVEEMLQHAQNDLRILLLHLRPVELENKTLSEGFRMILKE
                     LTDKSDIEVVYHESILTLPKKIEDNIFRIGQEFISNTLKHSQASRLEVYLNQTENELQ
                     LKMIDNGIGFDMDSVYDLSYGLKNIEDRVEDLAGNLQLLSQPGKGVAMDIRLPLVNQS
                     EDKNG"
     gene            57455..58096
                     /locus_tag="ACUBV3_000054"
     CDS             57455..58096
                     /locus_tag="ACUBV3_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HHU3846594.1"
                     /translation="MDKIKIVLVDDHEMVRLGLKSFLNLQADVEVIGEASNGLEGIKK
                     ALELRPDVVVMDLVMPEMDGVEATLALLKDWPEAAILVLTSYLDNEKIYPVIEAGAKG
                     YMLKTSSAAEILNAIRKVARGEQAIENEVDKKIKAHDKCPALHEGLTARERDILNLLA
                     KGYDNQRIADELFISLKTVKTHVSNILGKLNVADRTQAVVYAFQHHLVPQDDE"
     gene            58138..59538
                     /locus_tag="ACUBV3_000055"
     CDS             58138..59538
                     /locus_tag="ACUBV3_000055"
                     /EC_number="3.1.3.-"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional Cof-type HAD-IIB family
                     hydrolase/peptidylprolyl isomerase"
                     /protein_id="HHU3846595.1"
                     /translation="MDAKTKYKAKKIKAVFFDIDDTLRVKDTGYMPPSILKVFKALKD
                     KGIVVGIASGRARYGVPKEVQDLNADYCVKLNGAYVKDKDKNIIFHRPIPAEYVEQYK
                     KWADTVGIKYGLAGRHEAVLSDRDDLVNDAIDIVYSDLEVNPDFNKEHDIYQMWTFED
                     KGDSLHLPEPLDEHLRLIRWHDHSSDVVLKGTSKALGVSKVVEHLGLKPENILVFGDE
                     LNDLELFDYAGLAVAMGVSHPEAQKKADFITKKVEEDGILYALEELGLIEKELTFPQV
                     DIENTEGPVAVIKTNHGDMTVKLFPDHAPKTVANFIGLAKQGYYDGIIFHRIIPDFMI
                     QGGDPTGTGMGGESIYGESFEDEFSEELYNVRGALSMANAGPNTNGSQFFIVQNTKIP
                     YAKKELERGGWPTPIAELYAGQGGTPHLDRRHSVFGQLVNQSSFEVLDEIAAVETGSQ
                     DKPLEDVVILTIEVKE"
     gene            59540..59896
                     /locus_tag="ACUBV3_000056"
     CDS             59540..59896
                     /locus_tag="ACUBV3_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HHU3846596.1"
                     /translation="MKIGDKLKGTVTGIRPYGAFVSLEDGRTGLIHISEIKTGYIDNI
                     YDVLSVGDEVYVQVIDVDEFTQKASLSLRTLEEERHHIQHRHRFSNNRLKIGFKPLEE
                     NLPSWVEEGLAYLNNK"
     gene            complement(59943..60719)
                     /locus_tag="ACUBV3_000057"
     CDS             complement(59943..60719)
                     /locus_tag="ACUBV3_000057"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HHU3846597.1"
                     /translation="MTNEKGIVFNIQHFSIHDGPGIRTTVFLKGCPLRCPWCANPESQ
                     KMVPETMRDAITNESVIVGEEKSVDDIIEEVLKDIDFYEESGGGITLSGGEIFAQFEF
                     AKAILKRAKSLGIHTAIETTAYTRHEQFIDLIQYVDFIYTDLKHYNSLKHQEKTMVKN
                     ASIIKNIHYAFANGKTIVLRIPVIPNFNDSLEDAEEFACLFDRLDIRQVQLLPFHQFG
                     QNKYQLLNRQYEMEEIAALHPEDLLDYQAIFSKYNIHCYF"
     gene            60840..61595
                     /locus_tag="ACUBV3_000058"
     CDS             60840..61595
                     /locus_tag="ACUBV3_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HHU3846598.1"
                     /translation="MNRLENIISLVSQYQKIDVNTLSELLQVSKVTIRKDLDKLEGKG
                     LLHREHGYAVLNSGDDLNVRLSFNHKTKKEIAALAANMVSDNDTILIESGSTCALLAE
                     NICQTKRNVTILTNSCFIANYLREYDSCQIVLLGGEYQSSSQVTVGPLLKKMISLFHV
                     SLAFVGTDGFDPKLGFMGRDLMRSEVAQEMANAADEVIILTDSSKFNQTALVEQLPLS
                     TVSQVITDKHPNSEIANLFQEAEITIQSLLQVP"
     gene            61714..62697
                     /locus_tag="ACUBV3_000059"
     CDS             61714..62697
                     /locus_tag="ACUBV3_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000764457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HHU3846599.1"
                     /translation="MKLERQKLLAKVAYLYYMEGKSQSEIANELGIYRTTISRMLAKA
                     REEGLVRIEISDFNPEIFQLESYFKSKYHLKDIEIVSSRKDSDTSEIEKDLAHVAAAM
                     IRKKIKENDKVGIAWGRTLSKVVEAMRPHPVSQVSFVPLAGGPSHINARYHVNTLVYE
                     MSRRFQGSCTFINATLVQENANLAKGILTSKYFEGLMDNWEKLDVAIVGVGGKPKSNE
                     QQWLDLLNQDDFQCLDEEAAVGEITCRFFNHSGDPVNQHLAKRTIGITLEQLQKVPNR
                     IAVAHGNYKAAALLAVLKKGYINHLVTDFSTALNILRLDKDTFVDTIYQKS"
     gene            62901..63224
                     /locus_tag="ACUBV3_000060"
     CDS             62901..63224
                     /locus_tag="ACUBV3_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HHU3846600.1"
                     /translation="MEIIVADQIIMGLILNAGDAKQHIYQALKLAKEGNFAESKIEIE
                     LADSALLEAHNLQTQFLAQEAGGTRTDISALFIHSQDHLMTSITEINLIKEIIDLRQE
                     LHIQK"
     gene            63241..63561
                     /locus_tag="ACUBV3_000061"
     CDS             63241..63561
                     /locus_tag="ACUBV3_000061"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HHU3846601.1"
                     /translation="MIKIGLFCAAGFSTGMLVNNMKIAADKEGIEAHIEAYSQGKIAD
                     YAKDLDVALLGPQVSYTLDKSKSICDEYGVPIAVIPMADYGMLDGVKVLKLALSLLET
                     RKEA"
     gene            63563..64864
                     /locus_tag="ACUBV3_000062"
     CDS             63563..64864
                     /locus_tag="ACUBV3_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HHU3846602.1"
                     /translation="MSKFDSQKIITPIMKFVNMRGIIALKDGMLAILPLTVVGSLFLI
                     LGQLPFKGLNQAIANVFGPEWTEPFMQVYSGTFAIMGLISCFAIAYAYAKNSSVEPLP
                     AGVLSLSSFFILMKSSYIPVKGEAIADAISKVWFGGQGIIGAIIIGLVVGAIYTWFIQ
                     HHIVIKMPEQVPQAIAKQFEAMIPAFVIFLLSMIVYLIAKVTTGGTFIEMIYDIIQVP
                     LQGLTGSLYGAIGIAFFISFLWWFGVHGQSVVNGIVTALLLSNLDANKSLLAANRLTL
                     DNGAHIVTQQFLDSFLILSGSGITFGLVIAMLFAAKSKQYKALGKVAAFPAIFNVNEP
                     IVFGFPIVMNPVMFLPFILVPVLAALIVYGAIAVGFMQPFSGVTLPWSTPAIISGFMV
                     GGWQGALVQIVILAISTAVYFPFFKIQDNITYKNECEMERV"
     gene            65045..67501
                     /locus_tag="ACUBV3_000063"
     CDS             65045..67501
                     /locus_tag="ACUBV3_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HHU3846603.1"
                     /translation="MTKQLLTQKYTNDVQQNSQKHFGYLTERMYSYRDKVLDKKPFID
                     AERAILVTEAYQKHQEKPNVLKRAYMLQNILEKMTIYIDDETMIVGNQASSDKDAPIF
                     PEYTLEFVVNELDLFEKRDGDVFYITEETKEQIRNIAPFWENNNLRARAGVMLPEEVQ
                     VYMETGFFGMEGKMNSGDAHLAVNYQKLLEEGLIGFEKKARKAKADLDLTKPESIDKY
                     HFYDSILITIEAVKTYAERFAILAKKQAKTANAKRRQELLDIASICERVPYYPAKTFA
                     EAVQSVWFIQCILQIESNGHSLSYGRFDQYMYPYVKADLEAGRETEDSIVERLTNLWI
                     KTITINKVRSQAHTFSSAGSPLYQNVTIGGQTRHKEDAVNPLSFLVLKSVAQTHLPQP
                     NLTVRYHANLDKSFMNEAIEVMKLGFGMPAFNNDEIIIPSFIKKGVSEEDAYDYSAIG
                     CVETAVPGKWGYRCTGMSYINFPKVLLITMNNGIDPASGKRFAPSYGHFTQMTSYKEL
                     KEAWDKTLRYLTRMSVIVENAIDISLEREVPDILCSALTDNCIGRGKHLKEGGAVYDY
                     ISGLQVGIANLSDSLAALKKLVFEEKRLTTLEVWQALQSDYAGPRGEEIRQMLINEAP
                     KYGNDDDYADSLVRECYDVYVEEIAKYPNTRYGRGPIGGIRYSGTSSISANVGQGRGT
                     LATPDGRHAGTPLAEGCSPSHNMDKKGPTSVLKSVSKLPTDEIVGGVLLNQKVNPQTL
                     AKEEDKQKLIALLRTFFNRLHGYHIQYNVVSRETLIDAQKHPEKHRDLIVRVAGYSAF
                     FNVLSKATQDDIIARTEHAL"
     gene            67511..68179
                     /locus_tag="ACUBV3_000064"
     CDS             67511..68179
                     /locus_tag="ACUBV3_000064"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HHU3846604.1"
                     /translation="MEFLLDTLNLEAIKKWHHILPLAGVTSNPTIAKKEGDIHFFQRI
                     RDVREIIGREASLHVQVVAKDYQGILDDAAKIRQETDDDIYIKVPVTPDGLAAIKTLK
                     AEGYNITATAIYTSMQGLLAISAGADYLAPYFNRMENLDIDATQVIKELAQAIERTGS
                     SSKILAASFKNASQVTKALSQGAQSITAGPDIFESVFAMPSIAKAVNDFADDWKASQH
                     SEHI"
     gene            68247..69335
                     /locus_tag="ACUBV3_000065"
     CDS             68247..69335
                     /locus_tag="ACUBV3_000065"
                     /EC_number="1.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008888 - glycerol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HHU3846605.1"
                     /translation="MKVFASPSRYIQGKDALFQSIEHIKSLGQTPLILCDDVVYNIVG
                     ESFLSYLQDDLLPHRVSFNGEASDNEINRVVAVAKEKNSDLIIGLGGGKTIDSAKAIA
                     DKVNLPVVIAPTVASTDAPTSALSVIYTDEGAFEKYIFYSKNPDLVLVDTQVIAQAPK
                     RLLASGIADGLATWVEARAVLQKNGIAMAGGRQTLAGVAIAQACERTLFNDSLQALAA
                     CDAKVVTKALENVIEANTLLSGLGFESAGLAAAHAIHNGFTALSGDIHHLTHGEKVAY
                     GTLTQLFLENRPKEEIDRYINLYQAIGMPTTLAELHLGDATYEELLKVGQQATIEGET
                     IHEMPFKISAEDVAAALLAVDRYVSNHQ"
     gene            complement(69487..70413)
                     /gene="cysK"
                     /locus_tag="ACUBV3_000066"
     CDS             complement(69487..70413)
                     /gene="cysK"
                     /locus_tag="ACUBV3_000066"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HHU3846606.1"
                     /translation="MSKIYNSITDLIGNTPIIQLHHIVPEGAADVYVKLESFNPGSSV
                     KDRIALAMIEDAEQKGILKAGDTIVEPTSGNTGIGLAWVGKAKGYNVIIVMPETMSIE
                     RRKIIQAYGAQLVLTPGSEGMKGAIAKAKEISVEQNAWLPLQFNNQANPEIHEKTTGR
                     EIIETFGEKGLDAFIAGVGTGGTITGVSRALKKVNPDVAIYAVEADESAILSGEQPGP
                     HKIQGISAGFIPETLATDSYDHIIRVTSDDAIETGRIIGGLEGFLAGISASAAIYAAI
                     EVAKQVGKGKKVLALLPDNGERYLSTSLYDFE"
     gene            complement(70504..71148)
                     /locus_tag="ACUBV3_000067"
     CDS             complement(70504..71148)
                     /locus_tag="ACUBV3_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001108146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HHU3846607.1"
                     /translation="MNYKTIKSDGIVEEEIKKSLFICHLKRVESEEEGRNYITQIKKA
                     HYKANHSCSAMVIGEKGDIKRSSDDGEPSGTAGIPMLTVLEKQGLTNVVAVVTRYFGG
                     IKLGTGGLIRAYSGSVANAIKEIEVVEVKEQIGIRIQLTYPQYQTFDNFLKEHHLQEF
                     ETEFLEAVTCKIYVDPKEFEHTITNLTEFYQGKALLTEEGSQIVEIPFKDETDK"
     gene            71204..72493
                     /locus_tag="ACUBV3_000068"
     CDS             71204..72493
                     /locus_tag="ACUBV3_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HHU3846608.1"
                     /translation="MENYLGRLWTKAQLSEQLRKIAINLPSFIKKGSDYICTRCSSSV
                     AKNCQLPTGNYYCRECIVFGRVTSNENLYYFPQKTFSKTNSLKWKGGLTPYQNEVSEE
                     LLKGISSKENLLVHAVTGAGKTEMIYHSIAKVIDTGGSVCIASPRIDVCLELHKRLSN
                     DFRCAITLMHGESPSYQRSPLTIATTHQLLKFYHAFDLLIVDEVDAFPYVDNPILYHG
                     VKQALKENGTSIFLTATSTTELERKVARKELKKLHLARRFHANPLVIPEMVWVSGIQK
                     SLQTQKLPPKLYQLINKQRQTRYPLLLFFPHISEGQVFTEMLRQAFPMEKIGFVSSKS
                     TSRLKLVQDFRDNKLSILVSTTILERGVTFPSVDVFVIQANHHLFTKSSLVQISGRVG
                     RALERPEGLLYFLHDGKSKSMHQAIKEIKNMNHIGGF"
     gene            72493..73158
                     /locus_tag="ACUBV3_000069"
     CDS             72493..73158
                     /locus_tag="ACUBV3_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000128288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HHU3846609.1"
                     /translation="MTCLLCHEIDLSQLTFVELMLLKPKQNVICQTCKGSFEALSREM
                     GCQTCCKQIPQKQCQDCIYWGKKGIEVNHFSLYRYNEAMKKYFSLFKFQGDYLLKDVF
                     TKEIKAALKRYKGYTIVPVPLSHEGYQNRQFNQVIAFLQSANIPYKNILSKKDRGKQS
                     ANNKEERLKQVQQFTLKNEAELRDNLLIVDDIYTTGATIAQIRKLLEEKGIKNIKSFS
                     LAR"
     gene            73235..73789
                     /gene="hpf"
                     /locus_tag="ACUBV3_000070"
     CDS             73235..73789
                     /gene="hpf"
                     /locus_tag="ACUBV3_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000599095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation-promoting factor, HPF/YfiA
                     family"
                     /protein_id="HHU3846610.1"
                     /translation="MIKYSIRGENIEVTEAIREYVETKLSKVEKYFNEEQELDTRVNL
                     KVYREKTAKVEVTILIDSITLRAEDVSQDMYGSIDLVVDKIERQIRKNKTKIAKKYRE
                     KIPASQVFTTEFEAEPDEEAVSQRIVRTKNVNLKPMDVEEALLQMELLGHDFFIYTDA
                     EDNTTNVLYKREDGELGLIEAKGN"
BASE COUNT        24213 a        11692 c        14907 g        23115 t
ORIGIN      
        1 tgtcaattat tattgggtta atggccgcta tgtttatcat tcgtttagct tatttaaagc
       61 tatctatagc taatgaaaag gccttaagaa aaaatggtgc taaagaatat ggtgtaggtg
      121 tttcaaaagc aatcacagtt cttcatatta tcatttattt ttcatctgta actgaggcta
      181 ttctaacaaa agcaagtttt aattttgtta gcgtaatcgg gcttagcttg atgatttttt
      241 ctgtgtttat gttacataca gtaacacgtt tacttggtcg tatttggaca gtgaaattaa
      301 tggtagataa aaatcatcaa tttgtagatc actggctatt tagggttgtt aagcatccta
      361 actatttctt aaatattgct cctgaacttt taggtgtaac tttattgtgt catgctaaat
      421 acacagcatt atttgtcttg ccaatttatg cttttgttat ttatttaaga attcgtgaag
      481 aaaacttatt attaaaaacg atcattattc caaatggaat caaaaagtct agggtatctt
      541 aagaataaga taaatagtag acataaaagt cattttttgt tatactgtct cagtaagaag
      601 aggtgttcat cagctgtttt tttagcgagc tcaaggtagt gaaagttggg tagaatagga
      661 tgaatattgg cactttttag tgagtaaaag tacaatcaaa aatgaagtaa taaattaggg
      721 tggaaccgcg tctttgacgc ccctgtgctg ttgggcatag gtggtgtagg acgtggtttt
      781 tatcatttat ccgttttgag tcaattgttg tcgtcttccc aacagctgta ataaagtatg
      841 aaagaaggaa aatgatgtct aagaaattaa cttttcaaga aattattcta actttacaac
      901 agttttggaa tgaccaaggc tgtatgttaa tgcaagctta tgataatgag aaaggagcag
      961 ggacaatgag cccttatact ttcttacgtg ctataggccc tgagccttgg aatgctgcat
     1021 acgttgaacc atcacgtcgt ccagctgacg gccgctatgg tgaaaatcca aaccgtcttt
     1081 accaacatca tcaatttcaa gtggtaatga agccttcgcc gtcaaatgtt caagaacttt
     1141 atttgaagtc tttggaatta ctaggtatca atccacttga gcatgatatc cgttttgttg
     1201 aagataactg ggaaaatcca tctacaggtt cagctggatt aggttgggaa gtttggcttg
     1261 atggaatgga aataacacaa tttacttatt tccaacaagt aggtggactt caaacaggtc
     1321 ctgtaacttc tgaagttact tatggactag agcgtttagc ctcttatatt caagaggtag
     1381 attctgttta tgatattgag tgggcaccag gtgttaaata tggtgaaatc tttactcagc
     1441 cagagtatga gcattcaaaa tattcatttg agatatcaga ccaagtaatg ttacttgaaa
     1501 actttgagaa gtttgaaaga gaagcaaaac gtgctttaga agaaggactt gttcatccag
     1561 cttatgacta cgtccttaaa tgttcacaca cctttaattt attagatgca cgtggagcag
     1621 tatctgttac tgagcgtgca ggctatattg cacgtatccg gaatttagca cgtgttgttg
     1681 ctaaaacctt tgttgccgaa cgtaaaaaac ttggtttccc acttttggat gaggaaactc
     1741 gtatcaaatt acttgctgag gaagactaat gaacacactc attgttaata gtcatcctga
     1801 tttttcgaat ccgtactcat ttacaactat tctacaagaa aagtttattg aactttataa
     1861 tgagcatttt cctaaccatc agttatctat tttaaattta tatgattgtg ttttgccaga
     1921 aattacgaaa gaagtactac tttcgatttg gtctaaacaa agaaaggatt tggaattaac
     1981 agccgatgag attgttcaag ctaaaatctc taaagattta cttgaacagt ttaagtcaca
     2041 tcaccgtatt gtctttgttt ctccaatgca caattacaat gtgactgcta gagcaaaaac
     2101 ttatattgat aatattttta ttgcaggtga gactttcaag tacactgaaa atggttcggt
     2161 cggattaatg acagatgact atcgtctgct tatgttagaa tctgcgggtt ctatttattc
     2221 taaaggacaa tatagtccat atgaatttcc tgttcattat ttaaaagcta tctttaaaga
     2281 ttttatggct tttgatgatt tctgtgttgt tcgtgcagaa ggtaccgata ttttagatag
     2341 gcaggttgtt cttgataaga tgaatcagga tttaagagaa gcatttgaag cgttttacag
     2401 taaggagtag gaaatgacta aagatttatt attggagctt ggccttgaag agttgccagc
     2461 ttatgttgtg acaccgagtg agaagcaatt gggccaaaaa atggtcaagt ttttagagga
     2521 tcatcgttta tcatttgaaa ctgttcaaac tttttcaact ccacgtcgtt tggctgttcg
     2581 tgttaaagga ttggcagacc agcagacaga tttaacagaa gatttcaagg gtccttctaa
     2641 gaaaattgct ttggatgcag aagggaattt ttctaaagca gcccaaggtt ttgttcgcgg
     2701 aaaaggttta agtgttgatg atatcgaatt tcgtgaagtt aagggagaag aatatgttta
     2761 tgtcactaag catgaaactg gaaaaagtgc gattgatgtt ttagcaagtg tgacagaggt
     2821 attaacagaa ttgactttcc cagtgaacat gcattgggca aataatagtt ttgaatatat
     2881 tcgtccagtt cacactttag tggtattatt agatgatcaa gccttagagc tagacttcct
     2941 tgatattcat tcagggcgta ttagtcgagg gcaccgtttc ttgggttctg acactgaaat
     3001 tttatctgct agttcgtatg aagatgactt gcgacaacaa tttgttattg ctgatgcaaa
     3061 agaacgccag caaatgatcg ttgatcaaat tcatgctatc gaagaaaagg aaaatatttc
     3121 agttgaaatt gacgaagatt tactaaatga agtgcttaat ttagttgagt atccgacagc
     3181 attcttgggt tcttttgacg agaaatacct tgatgtacct gaagaagttt tagtcacttc
     3241 aatgaaaaat catcaacgct attttgttgt tcgcgacagg gatggaaaat tgttaccaaa
     3301 ctttatttct gtgcgtaatg gaaatgctga gcatatcgaa aatgttataa aaggtaatga
     3361 aaaagtttta gtggctcgtc tcgaagatgg tgaattcttc tggcaagaag atcagaaact
     3421 taatattgca gatttagttg agaaattaaa acaagtaact ttccatgaaa aaattggttc
     3481 actttatgaa catatggatc gtgtaaaagt tatttcacaa tatttggctg agaaggcaga
     3541 tctttctgat gaagaaaaat tagctgtgct tcgagctgca agtatttata aatttgacct
     3601 cttaactgga atggttgatg agtttgatga attacaaggt attatgggtg agaaatatgc
     3661 cttacttgca ggggaacagc ccgcagtagc agcagcgata cgtgaacatt atatgccgac
     3721 atcggccgat ggagagcttc cagaaaccag agttggtgca attctagcgt tagctgataa
     3781 atttgacacc ttactttcat tcttctcagt tggtttgatt ccaagcggtt caaatgatcc
     3841 atatgctctt cgtcgagcta ctcaggggat tgtccgtatt ttggaagctt ttggttggga
     3901 tatcccactt gatgaattag taactaacct ttatggtcta tcgtttgcta gtttagatta
     3961 tgctaatcaa aaagaggtca tggcctttat ttcagctcgt attgaaaaga tgattggctc
     4021 taaggtccct aaagatattc gagaagccgt tttagaatca gacacttata ttgttagcct
     4081 tattctagaa gctagccaag cattggtgca aaagagcaag gatgctcaat ataaagtttc
     4141 cgttgaaagt ttgtcacgtg catttaattt agctgaaaaa gttactcatt ctgtatcggt
     4201 ggactattca ttgtttgaaa ataatcagga gaaggcgcta tatcaagcta ttttgtcact
     4261 tgaactgact gaggatatgc atgacaactt agataagctt ttcgcactta gtccaattat
     4321 taatgatttc tttgataata caatggtaat gacagatgat gaaaaaatga aacaaaatcg
     4381 tctggcttta ctcaatagcc ttgttgcaaa agcaagaaca gtcgctgctt ttaacttatt
     4441 aaacacaaaa taaggaggca ttttatggat cctaaaaaaa ttgctcgtat taacgagctt
     4501 tcaaaaaaga aaaaaacagt tggattgacc ggagaagaga aggttgaaca agcaaagctc
     4561 cgtgaagaat atattgaagg tttccgccga tcagtccgtc accacgttga aggaattaaa
     4621 ttagtggatg atgagggcaa tgatgttacc ccggaaaaac ttcgtcaagt ccagagagaa
     4681 aaaggactac atggtcgtag tttggatgat ccaaattctt aatggggaga caattttaga
     4741 ttaaagtcaa cacttgggtg gggcaaaaga taatctttgt ctcgcctttt ttattaggaa
     4801 ggaaaaaata atgattgaat tactattagc attactgtta tggaatattt tagtatttgc
     4861 aacctatgct attgataaaa ggcgtgcaat tagaggggca tggcgtatta gtgagaaaac
     4921 attgttaata gagagtttat tattaggagg atttggtgca ttcctaggtg gagaagtttt
     4981 tcgtcataaa acgactaaat ggtatttcag actagtttgg tatatcggaa tgattattga
     5041 tttagctatt atttggctaa tttggtgtta ttgatgatat cttatcaaaa attttagggg
     5101 catattaaat ttttgatagt gcaggataga ctgatttagg tataatagaa ttacaaaaga
     5161 aaacgcttac ttttaaggag gaagtttatg tcttcagaag aaaaatacat tatggcgatt
     5221 gaccagggaa caacaagttc tcgagcaatt atttttaata aaaaaggtga aaaaattgcg
     5281 agtagccaaa aagaatttcc ccaaattttt cctcaagcag gctgggtaga gcataatgct
     5341 aatcagattt ggaattctgt tcaatctgtc attgctggag cttttattga atcaagcatc
     5401 aaacctggac agatagaggc tattgggatt accaatcaac gtgaaacaac tgttgtttgg
     5461 gataaaaaaa caggtcttcc aatttataat gctattgtat ggcaatcgcg acaaacagcc
     5521 ccgattgctg atcaattgaa gcaagaaggt catactaata tgattcatga gaaaacaggt
     5581 cttgtcattg atgcttactt ctcggcaaca aaagttcgtt ggattttaga ccatgttcct
     5641 ggagcacagg aacgtgctga aaaaggagag ttgctttttg gaacaattga tacttggtta
     5701 gtttggaagt taactgatgg tctcgtccat gttacagact attcaaatgc tgctcgtacg
     5761 atgctttata atattaaaga acttaaatgg gatgatgaaa ttttagagtt actgaatatt
     5821 cctaaagcaa tgttaccaga agtaaaatct aattctgaag tttatgggaa aactactcca
     5881 ttccattttt acggtggaga ggtgccaatt tctggtatgg ctggtgacca acaggcagca
     5941 ttatttggac agttggcctt tgaacctggt atggtaaaaa acacttatgg tacaggttcc
     6001 ttcattatca tgaatacggg cgaagagatg caactgtctc aaaataatct attaacaacg
     6061 attggttacg gcattaatgg taaggttcat tatgcacttg aaggatcaat cttcattgca
     6121 ggaagcgcta ttcaatggtt acgtgatggt cttagaatga ttgaaacgtc aagtgagtca
     6181 gaaggccttg ctcaatcatc aacaagtgac gatgaggttt atgtggttcc agcttttaca
     6241 ggtttgggtg caccgtattg ggattcaaat gctagagggt cagtatttgg attaactcgt
     6301 gggacaagta aagaagattt tgttaaagct acacttcagt caattgctta tcaagtacga
     6361 gacgttattg acactatgca agttgattca ggaattgata tccaacaact tcgtgtagat
     6421 ggtggagctg caatgaataa tttattaatg caattccaag cggatatttt agggattgat
     6481 attgctcgtg cgaagaacct tgaaactact gccttgggtg cagctttctt agccggactt
     6541 tcagttggct attgggaaag tatggatgaa ctaaaagaac taaacgcaac aggacaactt
     6601 ttccaagcaa caatgaatga gtctcgaaaa gaaaaactgt ataaagggtg gagaaaagct
     6661 gtcaaagcga cacaagtatt tgctcaagag gattaagggg gtgtagagat ggaattttca
     6721 cgagaaacac gacgtttagc cttacaaaga atgcaagatc gtactctgga tttacttatc
     6781 ataggtgggg gtatcacagg cgctggtgtc gctttacaag cagcagctag tggattagat
     6841 actggactta ttgaaatgca agattttgca gaagggacaa gtagtcgctc aacaaaattg
     6901 gtccatggtg gtcttcgtta cttgaagcaa tttgatgtag aagttgtttc tgatacagta
     6961 tcagagcgtg cagtcgtgca gcaaattgca ccacacattc ctaagccaga tccaatgctt
     7021 ttaccggttt acgatgagcc gggttctact tttagtatgt ttcgccttaa agtggctatg
     7081 gatttatatg atttattagc aggtgtgaca aatacacccg cagccaataa agttttgagc
     7141 gcagaagatg ttcttaagcg tgagcctgat ttacaaaaag aaggtttgct aggcggaggg
     7201 atctatttag atttccgtaa taacgatgca cgtctagtga ttgaaaatat taaacgtgct
     7261 aatagggatg gagcttatat tgctagtcat gtcaaagctg aggacttctt atttgatgac
     7321 aacaatcaaa ttataggagt acgtgcgcgt gatttgttga ctgaccaagt tatcgatata
     7381 aaagctagac ttgttattaa tacaactgga ccttggagtg acaccgttcg caacttctcc
     7441 aatgaaggta agcaaattca tcaattacgt ccaactaagg gagttcactt agttgtagat
     7501 cgccaaaaat taaatgtttc tcaacctgtt tatgttgata caggtctaaa tgatggtcgc
     7561 atgatttttg ttttgccacg tgaagataag acttattttg gaactacaga taccgattac
     7621 catggagact tagaacatcc tacggtaact aaagaagatg ttgactacct ccttaatatt
     7681 gtaaacaaac gtttcccaga agcagagctt actattgatg atattgagag ttcatgggca
     7741 ggtctgcgtc cactactatc agggaatagt gcctctgact ataatggagg caatagtgga
     7801 aaactaagcg atgaaagttt tgaagaatta attgattccg ttaaagatta tattgcacat
     7861 aaaaatcatc gtgaggatgt tgaaaaagct atttctcatg ttgaatctag tacttctgaa
     7921 aaagaattgg acccatcagc tgtttctcga ggatcaagtt ttgagcgtga tgataatggt
     7981 ctattaacac ttgctggtgg taaaattact gattatcgaa aaatggcaga aggtgccatg
     8041 gaaactatta ttaacatttt ggataaagaa tataatcgca agtttaaact aatcaattct
     8101 aaaacctatc ctgtttcagg aggagagatt aacccatcta atgttgattc tgaaattgaa
     8161 gcttatgctc aattgggcac actaagtggt ttaagtattg aggatgcacg ttatattgct
     8221 aacctttatg ggtcaaatgc accaaaatta tttgcattga cacgccaaat tacagaagca
     8281 gaaggtctga gcttggtaga aacgttgtca cttcactatg ctatggatta tgaaatggcg
     8341 cttagcccaa cggatttctt cttaagacgt acgaatcata tgcttttcat gagagataat
     8401 ttagattctt tgattcagcc tgttattgat gagatggcta aacattatca atggtctgat
     8461 caagacaaaa ccttctatga agaagaatta catgagactc taaaagacaa tgacttggca
     8521 gctttgaagg ataattgaag gagataataa tggatatttt tggagaattc ttaggaacag
     8581 ctctcttagt cttacttggt aatggtgttg tagcaggtgt tgttttgcct aaaacgaaga
     8641 atcataattc tggatggatt gttatcacat ttggatgggg gcttgctgta gctattgcag
     8701 tgttggtatc tggcaatatt agccctgccc acttgaatcc agctgtgtca ttagcttttg
     8761 caatcaaagg tgatttagct tggggaacag ctattcttta tatgattgca cagattattg
     8821 gtgcaatgct aggttcactt cttgtttatt tgcaatttag accccattat gaggctgcag
     8881 aaaataaagc agacatcctt ggaacttttg caactggtcc ggctttgaag gataattttt
     8941 ccaatttttt aagtgaagta ttgggaacac ttgttcttgt acttactatt tttgccatcg
     9001 gaaaatacaa tatgcctcca ggagttggta cgatgagtgt tggtatgtta gtggttggga
     9061 ttggtctatc gctcggtgga acaacaggtt atgctattaa tcctgcacgt gattttggtc
     9121 ctcgtttatt gcatgccttg ttaccgatga agaacaaagg tgattctgat tggacttatt
     9181 catggattcc gatagtagga cctatggtag gagctatttt agcagcctta atatttgcta
     9241 tgatgtaaga gatttttgat gctgagaccg actttatggt ctcagttttt tgttaccgtt
     9301 ttcaaaagta acttcttttg ttataataag taattatgac agaaaaatat gtaaataaag
     9361 taattgtcat tttaggagct tcttttgctg gtatgacttg tgcgcagaaa ttacgccaat
     9421 taaatcctaa ttgggatatc gttttgattg ataaagaaat tcatccagat tatgttccaa
     9481 atggcttaaa ttggtattat cgtcacgaaa ttagtggttt aaatcaggcc atgtggcaga
     9541 cagaagaaga gcaacggttg cagaatatac gctgtctatt tggtctaaaa gttgagaaaa
     9601 ttaataaaga agatagggaa ttaatgttat cagatggttc ttctgtctat tatgatcaat
     9661 tgatttgtgc catgggctcc caagcagaat caacttatat tgatggggct gatgcgcaag
     9721 gtgttttaac aacaaaaact tatgcaacta gtcaaaatgc taagcaggta ttagataaaa
     9781 gtcataaggt agcagttgtt ggggcaggta tcatcggttt agatattgct tatagtttac
     9841 atgaatcagg gaaggcggtc actttgttag aagctcaaga aagaccagac ttcaggcata
     9901 cagatcctga tatgtccctt cctttacttg atgcgatggc tgaatcaaaa ctacattttt
     9961 tccaaaatca aaaggttgag aaaattactg taacccgaga agaaaaactt tgtttgcgaa
    10021 cgctaacagg agacaccttt acagttgatg ctgtcatatt agctgttaat tttcgtccgg
    10081 atagtcgtct tttaactggg ttggttgatt taagtgttga taatagtgtt gttgtaaatg
    10141 attatttcca aacgagtgac cctaatatct atgctattgg cgatttgatt tggtcgtatt
    10201 ttaaaggtct taatagtgct tattatatgc ccttaattaa ccaagcaatc cgttcggcac
    10261 aaatgttggc ataccattta tcgggacatg ctgttccaaa attaaaaata acacgcgcta
    10321 caggatctaa acactttggc tattatagag ctaacattgg attaactgag ctggaagctg
    10381 gtctttacga ggataccgtt agtgttactt atttccctaa agaacagtat gatttaagga
    10441 taaaattgat tgctaatcaa aaaacgggcc atttattggg ggctcaatta atctcaaagg
    10501 aaaattgttt ggctactgcc aatcagcttg ttcaagctat atcgtgtgac atgacagatt
    10561 ttgatttggc ttttcaagat ttcatttata cagctagaga atctgagatg gcttatatgt
    10621 tacatcaagc agctatcaac ctttatgaaa agaggattgg gctatgcgaa tagaagctct
    10681 aatggaaaaa gaaaggcgtg ttcaatatcg gctactttct tttttaagag gctctcctca
    10741 ggcaatagca ctgaagttag ctttattaga aactggttta agtcgagcaa ctttgcttaa
    10801 atatatcaat aatcttaata gctactttga acaagaaaaa gttaattgtc gaatcgttta
    10861 ttataaggat aagttatttt tagaagagga ttataactta tccaatcaag aagtgttgaa
    10921 agctctgatg aaagatagta ttaaatacac tatcttaata tctttattta atcagcgtca
    10981 gttcacaatc gttggtcttt ctcaagaatt gatggttagt gaggctactt taaatcgtca
    11041 tcttgctcat ctcaatgaat tgttagcgga atttgatatt gctatttcac aaggaaaaca
    11101 gattggtgat gaattgcaat ggcgttattt ttattatgaa ttattcaagc aactatggtc
    11161 ttatgacaag tgccaaaata tgataaagaa attagactta gactcattaa ttctattaat
    11221 agagcgcttg gctcaacata ctttaactcg agaagctcag caaaatctgg ggctttggtt
    11281 tagtatttgt catcatcgtc tccttgctat ggaaaaaata tctgataatc tcaagcccat
    11341 tgtggaacat tatcaatgta atgcttttta taaacggtta gacgcagcct tagttttgta
    11401 tatgagtcgt tttgctcttg agtataggga aggagaagtt ctggcaactt ttgcttttct
    11461 acattcacaa aatattttac caatcaatac aatggaatac atcatggggt ttggaggtcc
    11521 gattattgat tgtgtaaccg aaacaatcat ctactttaaa aaggagagta ttttagctga
    11581 cgagacatca gatcaggtta tttaccaact tggtcagtta tatagccatt attatttctt
    11641 taaaggtcat attttagttg aacaacctga tttagaacaa acgtatcgac ttattgatca
    11701 taatatgagg gataagttgc atcatatttc aaagaagatt attgcaaatg ttaatcgaat
    11761 tagaccttta acagaaggtg gttgttcttt gctgacttta catttgttgg aattgcttat
    11821 tttctcgaaa aatagtcaaa aaatgccctt taggattggc ctagatatga caggtaatgc
    11881 tgtggaacag tcattgttgg aatatcgcat tcgacaacat ttctctggta ataattctat
    11941 tcaagtcgaa ccttatgacg aaggaaaggg atttgatatg gttatttatc agagtcgctc
    12001 aagaccttat aaagccaaac taacctattg ccttaataaa ggtgcttcag agcgagaact
    12061 acaggaaata gattcactta tttatgacaa ttaatcccat tacttaattg aatggtttta
    12121 atccctccag aaattgttaa aacgtttacg tattttatgt tataatatca aagacgaaca
    12181 attttaaatt tagttgttaa aaacagaaag gttttatcat gacatttgac accattgatc
    12241 aattagcggt taatacagtc cgcacgcttt ctattgatgc tatccaagca gcaaattctg
    12301 ggcacccagg tcttcctatg ggagctgcgc ctatggctta tgtgctttgg aataaattct
    12361 taaatgtaaa cccaaaaaca agtcgcaatt ggacaaaccg tgaccgtttt gtgctatcag
    12421 ctgggcatgg ttcagctctt ctttatagcc tacttcattt agctggctat gatttatcaa
    12481 ttgatgattt aaaacaattc cgccaatggg gatcaaaaac acctggacat ccagaagtta
    12541 accatactga tggtgtagaa gcaacaactg gacctttagg gcaaggtatt gctaatgctg
    12601 ttggtatggc aatggctgaa gctcatttgg ctgctaaatt taataaacca ggctttgatt
    12661 tagttgatca ttacacttat acacttcatg gtgatggttg tttgatggag ggtgttagcc
    12721 aagaagctgc cagtcttgct ggacacttaa aacttggtaa attggtcctt ctttatgatt
    12781 caaatgatat ctcacttgat ggtccaacgt ctcaatcatt tacagaagat gttaaagggc
    12841 gttttgaatc atatggttgg caacatatct tagttaaaga tggtaatgat ttagaagcga
    12901 ttgctgcagc aattgaagct gctaaagctg aaactgataa accaaccatt attgaagtta
    12961 aaactattat tggatttggc gcagagaaac agggaacatc ttcagttcat ggagcgccac
    13021 ttggtgcaga aggtattact tttgccaaga aggcttatgg atgggaatat ccagacttta
    13081 ctgttcctgc tgaagttgta gctcgatttg cctcagatct tcaagcgcgt ggtgcgaagg
    13141 cagaagaggc atggaatgat ctttttgcta aatatgaagt tgagtatcca gaattagcag
    13201 ctgaatacaa agaagctttt gctggccaag ctgagacagt tgaattgaaa gctcatgacc
    13261 taggttcatc agtggctagt cgtgtttcaa gccaacaagc tattcaacaa ttatcgactc
    13321 aattaccgaa cctctgggga gggtcagcag acctctcagc ttctaataac acaatggtag
    13381 cagcagaaac agatttccaa gcaagtaatt acgctgggcg taacatttgg tttggtgtac
    13441 gtgaatttgc aatggctgct gcaatgaatg gaattgcgct tcacggtggg actcgtgttt
    13501 atgggggtac attctttgtc ttctcaaact acttgttacc agcagtacgt atggctgcac
    13561 ttcaaaactt gccaactgtc tatgttatga cacatgattc tattgctgtt ggagaagatg
    13621 ggccaactca tgagccaata gaacaattag catcagtgcg ctcaatgcct aatttgaacg
    13681 ttatccgtcc cgcagacggt aatgaaacaa acgctgcatg gcaacgagct gtatcagaga
    13741 cagatcgacc aacgatgctt gttttaacac gtcaaaactt accagttctt gaaggaacat
    13801 cagaattagc ccaagaaggg gttaataaag gtgcttatat tttatcagaa gctaaaggtg
    13861 aacttgatgg tatcatcatt gcaacaggtt cagaagttaa attagcttta gatactcaag
    13921 acaaacttga gtcagagggc atccatgttc gcgtcgtatc tatgccagca caaaacattt
    13981 ttgatgaaca agaggcttca tatcaagagc aagtgcttcc atcagctgtt acaaaacgtc
    14041 ttgctattga ggcaggatca agcttcggtt ggggtaaata tgttggttta aatgggctaa
    14101 cacttactat tgatacttgg ggagcatcag caccaggtaa cagaattttc gaagagtatg
    14161 gctttacagt tgagaatgct gtatcgctat ataaagaact ttaagagcat gaagaagttg
    14221 ggaaaaatac ctcaacttct tttttgtaag atataggatg gatttgagaa tagttttgat
    14281 agactgatag gtgatggaaa gcactctttt ttgaaaacgt ttgacaaaca ttccatagcg
    14341 cagtataatt gataaaacta ttgtatatat aaaataatac aactattatt aattatcttt
    14401 ttagaaaggt ttgtttatga agaaggagtg tcgtgatttt tatcggcaga tacagcatac
    14461 ttacaatgat atagctgtcc gtgaagatgc agtgctatca tcaatactat tatcagcatc
    14521 gaatggtttg ataaaaacta gcgatgtacc tcgtgttgct tatgagttaa ctcagcaatt
    14581 ggaaaataat gagatagaaa aatctttcga gtcattagct acagttaagg aattgaaaaa
    14641 atctgctaaa aaattgtctg agagatatga gcaaaataag gtaacaacgg gattgctttc
    14701 taaagtattt ggaaatcatt aggagataaa aggagatttt tatgattaag tttgaacatg
    14761 tttcgaaagt atacggggaa aaagaagctc tcagtgattt aaccctctct attaaagatg
    14821 gtgagatttt tggattaata ggtcacaatg gagcaggaaa aacaacgaca attagtattc
    14881 taacatctat tattgatgca acttatggcc aagtctatat tgatgaccta cttttgacag
    14941 aacatcgtga ccaaattaag aaaaaaatag gttatgtacc ggactcgcca gatattttct
    15001 taaatttaac cgcagaggaa tactggtact ttttagctaa aatttatgac gttgccccag
    15061 aagatattga agcacgtatc acaaaattgg tagacatatt tgaactagag gagcaacgct
    15121 ataatcctat tgaaagtttt tcacatggta tgcgtcaaaa agtcattgtt ataggtgccc
    15181 ttcttccaaa tcctgacatt tggattctag atgagccgtt aactggttta gaccctcaag
    15241 cttcttttga tttgaaagaa atgatgaaag aacatgctaa aaatggaaaa acagttattt
    15301 tttcaacaca tgttttagca gttgcagaac agttatgcga cagaataggt attttgaaac
    15361 aagggaaatt aatctttgtc ggtagcttag gtgagttaaa aatgcaatat ccagataaag
    15421 atttggaaac tatctatttg gaattagcag gacgtcaagc taatagggag ggataaagta
    15481 tgaattggtc acgtatctgg gaactcgtaa aaattaatat cctttattca aaccctcaga
    15541 ctctatcggc actaagaaaa aagcaagaaa agcatcctaa aaaagaattt tcagcttata
    15601 aatccatgtt tagaaatcag ttatttcaga ttttgctctt ttcaataatt tatgtatttc
    15661 tctttgtatc acttgatttt aaagaatatc cgggctattt cacgttctac attggtatct
    15721 ttacactagt atccattatc tactctttta ttgcgatgta cagtgttttc tatgagagtg
    15781 acgatgttaa acaatatgct tatttgccta ttaaatctga agagctctat gttgctaaaa
    15841 tttttgcaac atttgggatg tctgtaactt ttctaatgcc aatcctaacc ttaatgattg
    15901 tggcttattg gcgtataata ggtggcccac ttgcggtcct cctagcaatc ataaattttg
    15961 ctattttatt tttatcggta acggtaattt cattgtatat taatagttta ataggccgtg
    16021 ccattattcg aagtgctaat cggaaactta tttctactat attaatttca ctagctacat
    16081 ttggagcaat agttccctta ttatttgtga atatgactag tcaaaagatg gtacaaggga
    16141 aattacaaga tatcgctcca attccttatg tcagaggtta ttatgatatt gttacagcgc
    16201 cgtttagtat ggagagtctc ctcaactatt atcttccgct cttaatcatt ttattcttga
    16261 taggtgctat ttataaatgg gttatgccac gctactatca ggagttgttg tatggacagg
    16321 tcaagcagag aaaagtccat cgtcaaatcg attttagtaa acgtgagtca attaataaaa
    16381 ctcttgttaa acaccattta tcaagtttac aaaatgcgac attattgaca aatacatttt
    16441 taatgccact attatatcta gcaatgttca ttgttccaat tttgaataat ggtaaagaga
    16501 ttggtcgatt ttttaatgaa aattattttg ggatagcttt tctagcagga atattaatag
    16561 gtagtttatg tgtgatgcca gctagcattg taggggtagg tatttcttta gaaaaaagta
    16621 atttctactt tataaaaagt ttgcctatat cattttcgta tttcttgaag cataaatttg
    16681 taactttgat aaccttacaa cttgcagttc ctacttttat ttatttcttg gtaggattct
    16741 ttctacttaa gctatcaatc ctcgttttat tgagcttcat tttaggtcta gtcttcatgg
    16801 gacttatcga aggccagttt atttatagac gtgactataa acatctgttc ttgaattggc
    16861 aagaggttac gcaactattt aatagagggg taggccaatg gctgttagtt ggttcattgt
    16921 ttggaatgat gataataggt agttttctta ttggaataag tattttttgg tcaatggtat
    16981 ggaatactgt agctgtcaat atcataatcc ttattactgg gttgctcata ttatcaattt
    17041 gtcaatatct actgctaaaa aatttttgga agaagcttgt ttagtaaaaa gattttagaa
    17101 aaatcataca gtaagaataa tgcttaataa atgagatagt tatagcggat aaaataaagg
    17161 cttattgtgg actttatggt tggaaggcag ccactgtaat cctttgttta ataacgctag
    17221 ttttagctct aaaatatttg atttttattg ctatctatta tatcttatca gacaaagaga
    17281 ctagggataa cctagtctct ttgtctgatg ctaacttgtt tttcgtaata ttgtgctaga
    17341 atgaatgtca atatagcagt tgtgcttaca gtaatgataa ctgcaataag catggtaata
    17401 aaaagatgtg ttgaagtatg actgatataa agaaacagac ctggtagacc agaagagcct
    17461 acagcaaagc ggtgagcgtt cattataccg gcatatagtc ctccaagacc accaccaata
    17521 agtgaggcta ttagaggata acgtttcgga aggttgacac catataagat aggttcggtg
    17581 attcccatat aagctgttat ggcagcaggt ccagcaattt ctttaagctt tttttctttt
    17641 gttataagag tcaccactgt acctgcagtt gcttgtgcga tatttgagca gagcatgcct
    17701 ggtccaaaaa tagaatcaaa ccctaatttc gttagttgtg tgattcctaa tgcagcaatt
    17761 ccactatgaa caccaaacat gattaggata ggagcaaatg ctcctactag aaaagcaggt
    17821 gcccaactgg cgtatttgga taaaaaagta aaaataacag ctaaatattg gccagcgata
    17881 gtacccattg gtcccaatat agagaacgac aaaattccca tgctgagaaa tattaacata
    17941 ggaacaagca ctaaacgtag tgatttagga ataatttgat ttaaatattt ttctatataa
    18001 gcctgaacac aaataattag aataatggga atgacacttg atgcatagtt agtaagtgtg
    18061 aatgggatcg tatggaataa ggatactggt ttaccagaag ctactaaatt agcccaatta
    18121 gggtgaagga gcatgacaac agttcccaaa gctaaaatag gattagcttt caatttttga
    18181 gcagctgtga tagcgattaa gataggtaaa aaatagaaaa caccatctgc aaaaagattt
    18241 agtaatatat atgtttgaga taacggtgtc aagattttga atactagtaa taaagctagc
    18301 aatgccttaa tcataccagc gcctgataaa gctggtaaaa tcggttggaa agtcccagaa
    18361 acaaaatcaa taattcgact aactgctttg gcttttttag tttgttctgt tttagtcgtt
    18421 gagttagttt caatgaggga gttaatttct tcaaacactt ttgcgacatg agtcccgatc
    18481 actatctgat gttgccctcc gctgataacg acagttttta cctcttttaa agtttttaat
    18541 tgctcaaggt ttactttcgt cggatcattg agactaaagc gaaggcgtgt ttgacaatgg
    18601 taaacattgt taatattatt ttgcccacca aggtgtttga tgatattttt tgacaaggcg
    18661 atgtattctc tagacataat atctccttaa tacttataaa acgttttccg attctagatt
    18721 atagcatagt aataaaagaa aacacttgga aatgcttgtg aattttcaat gatgttttca
    18781 gatagcattc ctaaagggcc tatgatataa tttttttatg aaaagacatt ttgagacaac
    18841 tagaagaatt gttattaaag taggaaccag ttccctcgta cagacaagcg ggaaaattaa
    18901 tttaagtaaa atagaccatt tagcatttgt tatttctagt cttatgaata gaggcatgga
    18961 ggtgatttta gtatcatctg gagctatggg gtttggttta gacattttga aaatggataa
    19021 gcgtcctcaa gaaatatcac aacaacaagc agtttctagc gtaggacaag tagccatgat
    19081 gagtctttat tctcagatat tttcgcacta tcaaactcat gtttcccaaa ttttattaac
    19141 acgtgacgtt gtggtttttc cagagagttt gcaaaatgta acaaactctt ttgagagtct
    19201 gctctctatg ggaattttgc caatcgtcaa tgagaatgat gctgtcagcg tggatgagat
    19261 ggatcataaa acgaagtttg gggataatga ccgtctctct gctgtcgttg ctaaaattac
    19321 taaagcagat ttattaatta tgctgtcaga tattgatggt ctttttgata aaaatcctaa
    19381 tatttatgat gatgctgttt tacgtagtca tgtttctgag atcaccgatg acatcattaa
    19441 gtcagctgga ggagcaggaa gtaagtttgg tactggtggc atgcttagta aaattaaaag
    19501 tgcacaaatg gtgtttgata acaatggaca aatgatattg atgaatggcg ccaatcctcg
    19561 tgatatttta aaggtgttag atgggcataa tattggaact tattttgctc aaggaaagtg
    19621 agattgagtt atgacgtata ttgaaatatt agggcaaaat gctaaaaaag ctagtcaatc
    19681 agtagcacga ttaagtacag ctagtaaaaa tgagatactc agggacttag cgagaaacat
    19741 agtggctgat actgaaacca ttttgactga gaatgctcgc gatgttgcta aggcaaaaga
    19801 taatggtatc tcagaaatta tggtggaccg tctacgacta aacaaagacc gcatacaggc
    19861 gatagcaaac ggtatttatc aggtagcaga tttggcagat ccgattggtc aagttgttag
    19921 tggttataca aatttagatg gtttaaaaat ccttaaaaaa agagtgcctt taggggttat
    19981 tgcaatgata tttgaaagtc gtcccaatgt atcggtggat gcctttagtt tagcttttaa
    20041 aacagggaac gctattattt tacgtggtgg taaagatgct attttttcaa atactgcctt
    20101 ggttaattgt atgcgccaaa cattgcaaga tacaggccac aacccagata tagtacaatt
    20161 agttgaagat actagtcacg ttgtagcaga ggagttaatg caggcaacag actacgtgga
    20221 cgtcttaatt ccaagaggtg gtgcaaaatt aattcaaacg gtgaaggaaa agtcaaagat
    20281 tcctgttatc gaaacaggtg ttggaaatgt tcatatctat attgatgaat ttgcagattt
    20341 agatatggca gctaagattg ttattaatgc taaaacccaa cgccctagtg tctgtaatgc
    20401 tgctgaaggg ttagttgttc atcaagctat tgctaaggga tttttatctc aattggagaa
    20461 aatgctcaaa gaatcgaacc agtcagttga atttcgtgcc gatgaggaag ctttacaact
    20521 tttagaaaat gctgttgctg caagtgagtc tgattatgct actgaatttt tagactatat
    20581 tatgtcagtg aaagtagtag attcttttga acaagcaatt agctggatta ataaatacag
    20641 tagtcatcac tcagaagcta tcattactaa taatatcagt agagcagaaa tttttcagga
    20701 tatggtagat gcagcggcag tttatgttaa tgcttcaaca cgttttacag atggtttcgt
    20761 ttttggactc ggagcagaga ttggtatttc gactcaaaaa ttacacgcgc gtggaccgat
    20821 gggattagaa gctttaacaa gtacaaaata ctatattaat ggaactggtc aagtaaggga
    20881 atagttgacc tataatagga gagattgcat ttatcggtct ctcttttact tttctaaagt
    20941 ttaggattta tggtacaatt aaagttatga caaacgattt tcatcatatt actgtgcttt
    21001 tgcacgagac tgtggatatg cttgatatta agccagatgg tatctatgta gatgctactt
    21061 taggtggggc aggacacagc gaatatcttc tttcacaatt aggcccagat gggcaccttt
    21121 acgcatttga ccaagaccag aaggctattg acaatgctca tatacgttta aagaaatatg
    21181 tggataccgg tcaagtaacc tttatcaaag ataatttcag aaatctatct tctaacttaa
    21241 aagcattagg agtgtcggaa attaatggta tttgctatga tttaggtgta tcaagcccac
    21301 agctggacga gcgtgaacgc ggtttctcat ataaacaaga tgcccccctt gatatgcgaa
    21361 tgaatagaga gcagagcctt acagcttatg acgttgtgaa cacttatagt tatcatgatt
    21421 tagtaaggat tttctttaag tatggcgaag ataaattttc aaaacaaatt gctcgaaaaa
    21481 ttgagcaagt acgagctgaa aaaccaatat ctacaacaac agaattggct gagattatca
    21541 aatcgtctaa atctgccaaa gaactgaaga aaaaaggtca cccagctaaa caaatttttc
    21601 aagctattcg tattgaagtc aatgacgaac taggagctgc agatgaatct atacaacaag
    21661 caatggactt attagctgtt gatggtcgta tatctgtcat cacttttcat tctttagaag
    21721 atcgtcttac taagcaactt tttaaagaag catcaacagt agaagtacct aaaggtcttc
    21781 cttttatacc agatgattta caacctaaga tggagctcgt taatcgtaaa cccatcttac
    21841 caagtcaaga agaactagaa gcgaacaatc gtgcccattc agcgaaattg agagtagcgc
    21901 gtcgtattcg ataataagaa agaaacttat gacaaatgaa aaacgaactg aagcagtaac
    21961 acaaacttta caacgtcaca tcaagacttt ctctcgcatt gagaaagctt tttatggcgc
    22021 tattgtaatt actgctatta tcatggcagt cggtattatt tacttgcaaa gtaatagttt
    22081 acaagttaaa caagaagtaa atcaattaaa tagtaaaatc aacgataaac agacagaatt
    22141 tgacaatgct aaacaagaag taaatgagct ttctaatcgt gatcgaatta ctaaaattgc
    22201 taaagatgct ggtttaacaa ttcaaaacga caatatttat cggaaggtgg actagtctgt
    22261 gacttttttt aaaaagctaa aaaaaatctt ccttgattat gtgatccata taagagatag
    22321 gcgttcgcca cagaaaaata gggaaagagt aggtcaaaat ttgatgattt taaccatttt
    22381 cctatttttc atttttatca ttaactttgt catcatcgtt ggtacagata gtaaatttgg
    22441 ggttaattta tccaaggaag ccaagaaagt ctatcaacaa agtatgacag tacaggcgaa
    22501 gcgtgggaca atttacgacc gtaatggaaa tcctatcgct gaggatgcta cgacctatag
    22561 tctctatgct attatctcaa agaactatac gacagctaca ggtcaaaaac tatatgtaca
    22621 accttctcag tatgaaaaag tagctagtat cttagagaat aaattgggca tgaagaaaaa
    22681 tcttgtccta aaacaactca atcaaaaaaa attgtttcaa gtttcctttg gaagttcagg
    22741 ttcaggattg tcttatacca agatggctga catcaagaaa acgatggaaa agtcagatat
    22801 caaaggaatt ggtttttcta ccagtccggg gcgtatttat ccaaatggga tatttgcttc
    22861 acaatttata ggatttactc ttcctcagga tgatggagat ggaaaaaaat tagttggtaa
    22921 tacaggcctt gaagcggcac ttaacaaggt attatcaggt actgatggta aagtaacata
    22981 cgaaaaagac cgtagtggga atgttctttt aggtactgca acaacagaac gtagggcggt
    23041 taatggtaag gatatctaca caactttatc agagcctatt cagactgttt tagagactca
    23101 gatggatgtt ttcgcagaaa aaaccaaagg aaaatttgcg agtgctacag ttgtcaacgc
    23161 taaaacagga gaaattctag caacgagtca gcgtcctact tataatcctt ctacccttaa
    23221 aggttacgat aagaaaaatt tgggtaccta taacacacta ctatatgata atttttttga
    23281 acctggttca acaatgaagg taatgactct agcttctgca attgattcta agcattttaa
    23341 ctctactgaa gtgtataaca gtgcacaata taaaatcgca gatgcggtta ttcgagattg
    23401 ggatgtcaat gaagggttat cgtctggtag ttatatgact tttccacaag ggtttgcaca
    23461 ttccagtaac gttggtatgg taaccttgga acaaaaaatg ggacgagata aatggttaaa
    23521 ctatctttct aagtttaaat ttggttaccc aacacgtttt gggatgcttc atgaaagtgg
    23581 aggtttgttc ccatctgaca atgaagtaac tatcgctatg agctcatttg gtcaaggtat
    23641 tggtgtaacg caagttcaaa tgttgcgcgc atttacgtct atctcgaatg atggtgttat
    23701 gttacaacca cagtttatta gcagtattta tgatccaaat acagggactt ctcgtacagc
    23761 acgaaaagaa gttgttggaa aaccaatttc caaagaagca gctagtaaaa cacgtgatta
    23821 tatggttact gttgggacag atccttatta tggtacatta tatgcagcag gtgccccagt
    23881 tattcaggtg ggcaatcaat cagttgcagt aaaatctggt actgcccaaa tagctcagga
    23941 aggtggtggt ggttatctgc aaggaaagaa tgatactatt aactcagttg tggctatggt
    24001 gccatcggaa aatcctgact tcatcatgta tgttaccatc caacaacccg aaaaatttag
    24061 tatcacattc tggaaagatg ttgttaatcc tgttttagaa caagcaacag caatgaaaga
    24121 aacgatatta aaaccgggat tgaatgactc agaacatcag acaaaatata aactatcaaa
    24181 aatagttggt gaaaatccag gtcatgtagc ggaagaatta cgtcgtaatc tggttcagcc
    24241 aattatttta ggaaatggta gtaaagtttc taaagtatct aaaagaccag gagctaatct
    24301 tgctgagaat gaacagctac tagtattaac aaataaactg actgaattac ctgatatgta
    24361 tggttggtct aaagcaaacg ttgaacagtt tgctaaatgg acaggtataa aagtgaccta
    24421 taaaggttca acctcaggta aagtaagaaa acaaagtatt gatgttggaa aatccatcaa
    24481 taaaattaaa aaaatcaaaa ttacaatagg agattaacat gtttttaagt ataatggcag
    24541 gtgtcatagc atttgtcctg acagttattg ccattccacg cttcattaag ttttaccaat
    24601 tgaagaaaat tggcgggcaa caaatgcatg aagatgtcaa acaacatcta gccaaagcag
    24661 gtacgccgac aatgggagga acggtatttt tgattgttgc tcttcttgtc tcacttattt
    24721 ttagtattat tttatctaaa gaaaatagtg gtaatttagg tgcaactttt ggtattttat
    24781 ctgttgttct tatctatgga attattggtt ttttagatga ttttttgaaa atttttaaac
    24841 aaatcaatga gggcttgact ccaaagcaaa aaatgtcgct ccaactaatt gcgggtctaa
    24901 ttttttattt tgtccatgtg ttaccaagtg gcacatctgc gataaatatt tttggtttta
    24961 accttgaagt aggatactta tatgcttttt ttgttctttt ctgggttgtt ggtttttcaa
    25021 acgctgtgaa cctaacagat ggaattgatg gtctagcttc tatttcggtt gttatcagtt
    25081 tgattactta tggaatcatt gcttataatc aaacgcaatt tgatatttta ttgattatcg
    25141 ttatcatgat tggtgcttta cttggctttt ttgtctttaa tcataaacca gctaaagtct
    25201 ttatgggaga tgttggttca cttgcattgg gtgctatgtt agcagccatt tcaattgcct
    25261 tacgtcagga atggaccttg ctttttatcg gctttgtcta tgtttttgag acgtcttcag
    25321 tgatgttgca agtagcatac tttaagtata ctaagaagaa aactggtgtt gggaaacgta
    25381 tcttccgcat gacaccattt catcaccatt tagaattagg tggtgtgtct ggcaaaggta
    25441 ataaatggag cgaatggaaa gtagatgctt ttttatgggc tattggtatt ttcatgagcg
    25501 ctattacact tgctatttta tatctttaaa ctttattagg ggatgtggta tggactgcca
    25561 gattcccttt tgctttcttg tagcctttaa tggtataatg aacataataa tagtaaggaa
    25621 atagacatgt catttaaaga ttttaatttt aaaccataca ttcagagggc tctcgatgag
    25681 ttaaaattcg tcgatccaac tgatgttcaa gctaaattga ttccagttgt tcgttcaggc
    25741 cgcgatcttg tgggggaatc gaaaactggt tctggtaaaa cccacacctt tttacttcct
    25801 atttttgaga agctagatga aagtagtaat gatgttcaag ttgttattac tgcaccaagt
    25861 agagagttgg caacacaaat ttatcaggca actaaacaaa ttgctgagca ttcagagcaa
    25921 gaaatacggg tggttaacta tgttggtggt actgacaaat tacgtcaaat tgaaaagttg
    25981 aaagtaagcc aacctcatat tgtcattgga accccaggac gtatttatga tttggtgaag
    26041 tcaggtgatt tagctattca taaggcgcat acttttgtgg ttgatgaagc agatatgact
    26101 ctggatatgg gattccttga taccgttgat aagattgcag ggtctttacc caaggatgtc
    26161 caaattttag tcttttcagc aacgattcca caaaaattac agccattttt aaagaaatat
    26221 ttgaccaatc ctgtaatgga aaaaatcaaa acagcaacag ttatcactga taccatcgat
    26281 aactggcttt tgtccactaa aggacgtgat aaaaatgcac aaattttgga actctctaaa
    26341 ctaatgcagc cttaccttgc aatgatattt gttaatacga aagagcgtgc agacgaatta
    26401 cactcgtatc tttctagtaa tggcttaaaa gtagctaaaa ttcatggtgg gattgcacct
    26461 cgtgaacgta aacgtattat gaatcaggtt aaaaacctcg aatttgaata tatcgttgca
    26521 acagatttag cagcgcgtgg tattgatatt gaaggtgtta gccatgttat caatgatgcc
    26581 atacctcaag acctatcttt ctttgtccac cgtgtcggtc gcacaggccg taatggctta
    26641 tctggtactg ctataacttt atatcagcca agtgacgatt ctgatattcg tgaacttgaa
    26701 aaactaggta ttaattttat tcctaaagtc attaagaacg gtgaatttca agatacatac
    26761 gaccgtgatc gtcgtaataa ccgtgaaaag tcctatcaaa aattagatac agaaatgatt
    26821 ggacttgtga aaaagaaaaa gaaaaaaata aaaccaggtt acaagaaaaa aattcaatgg
    26881 aaagttgatg aaaagcgtcg caaagagcgt agagcaagta atcgtgctaa ggggcgtgcg
    26941 gagcgcaaag ctaaaaaaca aagtttttga aattattaaa gtctgaaatc agctcgtttc
    27001 aggctttttt attgatataa aatctttatc acctgataag gaaatactat tatacagata
    27061 gattttagag tgttagacta gtaatatgat attttaggag aattcttatg tttaaaaaaa
    27121 taatattatc tattgcaact atagtcgcaa ccgccagcct agcagtatca gttcaggcgt
    27181 cagagaaagt agaacttaaa gtagctacag attctgacac ggcaccattt acttatcaaa
    27241 aagacgggaa attcaaaggt tatgatgttg atgttgtcaa agctgttttt aaaggtagta
    27301 agtacaaagt aaccttcaag acagttcctt ttgatactat ttcaacaggt attgatgcag
    27361 ggaaatttga tttatcagct aatgattttt catacaataa agaaagagca gaaaaatatc
    27421 tcttctcaga gcctatatcc cgttcaaatt atgccgtagt agggaagaag gggagccatt
    27481 acaaatcatt aagtgacctc tctggaaaat caaccgaagt tttatctggc gttaactatg
    27541 cacaggttct agaaaattgg aataaaaatc atcctaataa aaaaccaata aaaatcaaat
    27601 atgtttctgg gacaactggt gttactagca gattaaaaaa tattgagagt gggaaaattg
    27661 actttatcct atatgatgcc atttcatctg actatattgt aaaagatcaa tcattaaact
    27721 taagcgtttc tcctttgaaa ggtaaaattg gtaataataa ggatggatta gaatacctcc
    27781 ttttaccaaa agataaaaaa ggtaaaactc tacagaaatt tataaataag cgtattaaag
    27841 ttttgaaaga agatggtact ttggcgcgtt taagtaaaca atatttcggt ggagattacg
    27901 tttcaaacat tgataagtaa atcagtgaga ctataagttt tatcttttta catgtcagag
    27961 tttgcgtgat agaataacga agataagaaa gtttagaaaa ggaaagtaga aggaatgaat
    28021 caatttatct tgacgggtgg atggtcttgg tataataatt tggttagtca ggttccagcg
    28081 gggaaattat ttagttggaa ggctgtttta gatgcaatac cctcaatttt agagcgatta
    28141 cccataacac tattattgac agtagcagga gccttatttg gactcatttt agctttgatt
    28201 tttgcaatag tgaaaattaa ccgtgtcaaa attttatatc cgattcaagc tttatttgtt
    28261 agttttttac gtgggactcc aatacttgtt caattaatgc tgagttatta tggaattcca
    28321 cttttcttga aatttttaaa ccaaaaatat ggttttgatt ggaacattaa tgctataccg
    28381 gcttctgtat ttgctattac tgcatttgct tttaatgaag cggcttatac aagtgaaact
    28441 atacgagcgg cgattttgtc ggttgatcaa ggtgaaattg aggcagcacg tagtttaggg
    28501 atgacctcag cgcaagttta tcgtcgtgtt attattccaa acgcagcagt agtcgcaaca
    28561 cctactttga taaatacgtt aattggtcta actaagggaa cctctttagc ttttaatgct
    28621 ggtattgttg aaatgtttgc ccaagcacaa attatgggtg gtagcgatta tcgctatttt
    28681 gaacgttata tctctgtggc acttgtttac tgggcggtta gtttcttaat tgaacaattg
    28741 ggaaatgcta ttgaacgaaa aatggcaatt aaggctccac gccatttaac tgatgagatt
    28801 ccaggaggag tacgctaatg ataaaattga gacaattgac taaatcattt tctggtcaaa
    28861 aagtgttaga caaacttgat ttagatattg aaaaaggtca agttgttgct ctagtaggtg
    28921 cttcaggtgc aggaaaatca acctttcttc gtagtatgaa ttatttagaa gagcctgatt
    28981 atggaacaat tgaaattgat gattttaaag ttgattttaa atcaatcagt aaagatgaca
    29041 tactaacatt acgccgtaag ttagccatgg tttttcaaca attcaatcta tttgagcgtc
    29101 gtacagcttt ggataatgta aaagaaggtt tgaaaattgt caaaaagatg tcagatcaag
    29161 aggcaacacg gattgcaaga gacgaactgg caaaggtcgg acttgcagat cgtgaaaaat
    29221 attatccaag acatttatca ggtggacaga aacaacgtgt tgctttggca cgcgcattag
    29281 ccatgaaacc tgatgttctt cttttagacg aaccgacatc agcacttgat ccagaattag
    29341 ttggagaagt tgaaaaatct attgcagatg ctgctaagca ggggcaaacc atggtacttg
    29401 ttagtcatga tatgaatttt gtttaccaag tagcagataa agttttattt ttggaaaaag
    29461 gtcgtatttt agagtctggg acgcctgaac aattatttaa ccatccttta gaagaacgta
    29521 caaaagaatt cttcgctagt tataacaaat cgtacctata agctaaaata ttttgatata
    29581 atgaaggtta gaatgtctaa tcttcatttt taattattct tgagttaggc taagtatata
    29641 agaaagctgc gatatgtttt gacaatcgct tgttggatag agatgttttt agaaatagca
    29701 aaagtttatt tacaaggttt agggcttgcc agcataactg ttattttagt tggtataatt
    29761 tgggtctttt ggcgtgccaa gaaaaacata gataaaacag ctagagaacg ccaatctgtt
    29821 ctttatgatg tccttctgat agctcttatg acagctccaa tacttgcctt tgcatttgta
    29881 gctatcattt taatgataaa agcttagtca gattagttgg aaatatagac gctttgctat
    29941 agaataaaac cataagattc aaaggagaaa tagtaatgta tgatacttta ataataggtt
    30001 caggtcctgg tggtatgaca gcagcgcttt acgctgcacg ttcaaacctt aaagttgggt
    30061 taattgaaca aggtgctcca ggtggacaga tgaataatac tgctgaaatt gagaactatc
    30121 caggctacga tcatatctca ggtccagaat tatcaatgaa gatgtatgag ccgttggaaa
    30181 aatttgaagt agagcatatt tatggtatag ttcaaagagt tgagaacgat ggtgatgtaa
    30241 agcgtgttat caccgaagat gaatcgtatg aagccaaaac agtaatcctt gctaccggag
    30301 ctaaaaacag ccttttagga gttccgggtg aagaggaata tactagtcgt ggcgtttctt
    30361 actgtgcggt ttgtgatggt gcatttttcc gtgatcagga tcttttagtt gtgggtggcg
    30421 gtgattctgc tgttgaagaa gctgtcttct taactcaatt tgctaaaagt gttactatta
    30481 ttcaccgacg tgatcaattg agagctcaaa aagtacttca agaccgagct tttgctaacg
    30541 aaaaaataaa atttgtatgg gattcagtgg tcaaagagat taaaggaaat gagattaaag
    30601 tatctggtgt aactgttgaa aatctaaaga ctggagaaat ctcagagatg cctttcggag
    30661 gtgttttcat ttacgttggt ttgaaaccac attctagcat ggtatcagaa ttaggtatta
    30721 cggatgagac tggctgggtc ttaactgata caaatatgaa aacatcaatt ccaggactat
    30781 atgctattgg ggatgttcgt caaaaagacc tacgtcagat tgcgactgct gttggtgaag
    30841 gtgctatcgc tggacagggt gtctataact atataactga gcatttctaa gaaatacaaa
    30901 gtagaaaaga ctatcgaaaa acgattagtc tttcttttta ttttcatgaa agtaaggtga
    30961 taaactgaac taccgagata gaaatatgct ataatgttag acatatagcc ttatcctagg
    31021 ctcatgatta aaaggagatt gtctgatgta taaagacgat agtttaacac tacatactga
    31081 tttatatcaa atcaatatga tgcaagttta ttttaataaa ggtattcaca ataaaaaagc
    31141 ggtgtttgaa gcttactttc gcaaggttcc atttgaaaat ggttatgctg tttttgcagg
    31201 tttagagcgt attgttcgtt atttagaaaa tctgagtttt tctgatagtg atttatccta
    31261 tttagaagag ttaggttatc ctgaagaatt tttagattac ttaaaaaatt taaaaatgga
    31321 actgactgtc aaatcagcca aggaaggtga ccttgtattt gctaatgaac cattagttca
    31381 aattgaaggt cctttagcac aatgtcagtt agtagagaca gcgattttaa atatcatcaa
    31441 ctaccagaca cttgttgcta caaaagcagc tcgtattcgt tctgttattg aagatgaacc
    31501 gctattagag tttgggacac gacgtgctca agaaatggat gcagccattt ggggtactag
    31561 ggcagcgatt attggtggtg ctaacgcaac aagcaatgtt cgtgctggta aaatctttaa
    31621 tattccagta tcaggaacgc atgcacatgc tttagttcaa acttatggcg atgattacca
    31681 ggcatttaaa gcttacgcag aaactcataa agattgcgtc tttttagtgg atacttatga
    31741 tacccttcgt gtaggggtgc ctaatgctat tcgagttgca aaagagatgg gcgaaaagat
    31801 taacttcttg ggagttcgcc ttgattcagg tgatttagct tatttgtcta agaaagttcg
    31861 tcagcagtta gatgacgccg gttttcctaa tgcaaagatt tatgcttcga atgatctaga
    31921 tgaaaataca atcctcaacc taaaaatgca aaaagctaaa attgatgttt ggggtgtcgg
    31981 gacaaaatta attacagcct atgatcagcc tgcgctaggt gctgtttata aaattgtttc
    32041 tattgagact gacgccggta gtatgcgaga tacaattaaa ctatcaaaca atgctgaaaa
    32101 agtatcaaca cctgggaaaa agcaagtttg gcgtatcacc agtcgagcta aagggaaatc
    32161 tgagggtgat tacatcacat ttgcagacac tgatgtgaca caattagatg aaattgacat
    32221 gtttcatcca acttatactt atatcaataa aacagtccgt gattttgacg cagtaccctt
    32281 actggtagat atctttgaca aagggaaatt agtttatcaa ttaccaagtc ttcaagaaat
    32341 tcaagagtac ggacgcaaag aatttgatca gctttgggat gagtataaac gtgttctgaa
    32401 tccacaggat tacccagttg atttggcacg cgatgtttgg caaaataaaa tggacctgat
    32461 tgatcgtatc cgtaaagaag ctttggcaaa aggagaagta agatgacttt gcaagatcaa
    32521 attatcaaag aactcggtgt aaaacctgtt attaatccaa gtcaagaaat ccgtcgttcc
    32581 gtagaatttt taaaagacta tctattaaaa catagttttc taaagaccta tgtactgggt
    32641 atttcagggg ggcaagattc aactttagca ggacgtctag ctcaactagc agttgaagag
    32701 ttgcgtgctg atacagggga aaactatcaa tttatagcta ttcgtttacc atatggtatt
    32761 caggccgatg aagaagatgc acaaaaagca ttagatttta tcaagccaga tattgctttg
    32821 accattaata ttaaagaagc tgttgatggc caagttagag ctcttaatgc agcaggagta
    32881 gagattacag actttaataa aggaaatatt aaagctcgtc aaagaatgat tagtcaatat
    32941 gcagttgcag gtcaatatgc tggagcggtt attggtactg atcatgctgc agaaaatatt
    33001 acaggttttt tcaccaaatt tggtgatgga ggtgctgatt tattacccct ctttagactt
    33061 aataaaagtc agggaaaaca gttattagct gaacttggtg ctgataaagc cttgtacgaa
    33121 aagattccaa ccgcagattt agaagaaaat aaaccaggaa tcgctgatga aatagcactt
    33181 ggagtaacct accaagaaat tgatgcttat cttgaaggga aggtagtttc ggacaagtct
    33241 cgagatatca ttgaaaattg gtggtataag ggtcaacata aacgtcatct tccaatcaca
    33301 atttttgatg atttctggaa ataagaaaaa tagctagaat attctagcta tttttgtttc
    33361 tctaataaat tactttcaga cttgcagaaa tattcagaat gtttataata gtagctaagt
    33421 ataaaaggag gaatttatgt cagaattaac tcaaacattt acagataagt tatttgctga
    33481 ttaccaagca aatacaaaat ttagcgctat tgaaaatgca gtaactcaca atggtttatt
    33541 gaagtctcta gagacacgtc aaagtgagat tgagaacgac tatgtctttt caattgattt
    33601 gaccaaagat gaagtctcta atcagaaaca atcaggacgt tgctggatgt ttgctgcact
    33661 gaacacattt cgtcataaac tgatttcaga ttttaaactc gagaactttg aattatctca
    33721 ggcacataca tttttctggg ataagtatga aaaatcaaat tggtttatgg aacaaattat
    33781 tgcgactgca aatcaggaat tgagtagtcg taaggtgaaa ttcctattag atgtacctca
    33841 acaagatggc ggacaatggg acatggtggt tgctctgttt gagaaatatg gtgtcgttcc
    33901 taaaacagtt tatcctgaat ctgtttcatc aagtgctagt cgtgaactaa atcaatattt
    33961 gaataaactc ttacgccaag atgcacagat tctacgagaa ttaatagcac agggagcaga
    34021 tggggcaact gttcaaaata aaaaggaaga attgcttcaa gaaattttta actttttagc
    34081 aatgaacctt ggcttgccac cgcaatcttt tgattttgct tatcgtgata aagacaatca
    34141 ttatcagtct gataaaaata ttacaccgaa agcattttat caaaaatatg ttaatcttga
    34201 cctatcagat tatgtttcta ttatcaatgc tccaactgta gataagcctt atggacaatc
    34261 ctatacagtt gagatgcttg gaaatgttgt cggagggcca gctgttaaat atttaaacct
    34321 tgatatgaaa cggtttaaag agttagcgat tgcgcaaatg aaatcaggag aaactgtgtg
    34381 gtttggttca gacgtaggtc aagtatcaaa tcgtcaaaag ggtatcctag caactacgac
    34441 ttatgatttt aattcatcta tggatattaa acttagccaa gacaaagcgg gccgcctaga
    34501 ttatagtgaa agtttaatga cacatgctat ggtcttaacg ggagtagacc ttgatgaatc
    34561 tggacaacct ttaaaatgga aagttgagaa ctcttgggga gagaaagttg gtaaggatgg
    34621 ttattttgta gcttcagatg cttggatgga tgaatatact taccaaatag tcgttcgtaa
    34681 agaattgctt actaaagaag aattagaggc ttacaacgca gagccaatta ctttagcgcc
    34741 gtgggatccg atgggagcac tagctaatta gtaatagtaa ttataaatta aaaaactgga
    34801 tatattatcc agttttttta attaccgtta ggtgctgtat tgctgttacc attaccatgt
    34861 ccagttcctg gcacagtttg attagaatta ttaggggtag tagcattacc attattaggt
    34921 gtttcgactt gtggggatga tgaattggca tcatttgctg tattcttatc ctctttttgt
    34981 ttctcagagg atgttgaact actgctttga cttgactgtc cagaattttg atagatattc
    35041 ttatagacag aagaagagta agtgccggtt gtagttgtcc cattgatata caagtagctt
    35101 ccgcttcgat agagcccttc tggcattgtc caatctgcac tgtatccgcc agttaggtag
    35161 gacatcattg cacgataaac ctcagtagca atatccagtt ggctaccata aagtggtgta
    35221 aggcgatttt tataaccagt ccaaattgcc attgtgtact tagaagtata gccgacaaag
    35281 ttttcatcag gagccattgt accaacggcg ctattgtaga taccagtagt tgcttcaatt
    35341 ttagctaact catcttccgt atagttggaa gtaccagtct taccagcttg tgcaacacca
    35401 gggatagctg ctttagtacc agtaccaaat gttagtactg ttttcagcat atccgtcatc
    35461 atgtaggcag tagtctcttt catcgcacgg ctaccagacg ctgcataagt atcattggtt
    35521 ccatcgctaa attcaatttt attaacatat tgcggcttat agtaagttcc gccgtttgca
    35581 aaagccgagt aagccgctgc cattttctca ctacttgcac catattttgc atcactgcta
    35641 ctgttgttac ttgaaatagc atttgaatag ttcatttctg gatagtatat gcctaatttt
    35701 tcaaggaaag attttgcttc gtctaatcca gcggcttcaa gcgctctgac agcaggcacg
    35761 ttacgtgatt gttgaatagc ggtctgcata ctcatccaac ccatatattg acgatcccag
    35821 tcatatagtt gagtagaagt accaggccag taataaactg agtcgtttaa tgattgacct
    35881 gttgaattat agacaccact atcaatagca ggtgcataag ctgagatagg tttcattgta
    35941 gaaccccaat cgcggtctgt taagacagat tgatttgtcc caaatgaaat attttcattc
    36001 tgatgacgcc cgcctaattg tgcaatgact ttaccattag tcgcatccat gatggtagat
    36061 gctatttgta attcattgtt tggataagcg atgtaagtgt cactgttgta gatgtcatat
    36121 agttgttttt gtgcatcagt gttgatatta gtatacactt ttagcccagc agtaaagata
    36181 tctttaccag ttttttgttt aacttcacta ataacttgtt ttaagtagtt atccatatat
    36241 tttggataag tagatttttg ctttaattct tttaagccat cagttactgg agttgcaaca
    36301 gcttggtcgt attccttttt agaaatgttt ttatcttgat acatctgctg aagaacggta
    36361 ttacgtcttg tttgagcaga ttctgggttt ttataagggt catattgtgt aggtgcttga
    36421 ggaataccag cgagcaaagc aagttgtgca atagataatt cctttaggtc tttaccaaag
    36481 tatgatttag ctgtcgttct cataccataa ttaccatttc ccatataaac tttattaata
    36541 tagaaagtaa gaatttcttc tttggtgtat ttacgctcca tttgaagcgc aagccaaact
    36601 tcctgtgatt tacgttttaa cgtttggtca gatttattgg tagaaaagta agccagttta
    36661 atcaactgtt gatcaagggt tgaaccacct tgcgtattac tactaacaag attatgccaa
    36721 gctgcaccta aaatacgata aatatcgaca cctctatgtt taaagaaacg tttatcttcg
    36781 atagaagtga tagcgttaac caaatttagt ggaatgctat ccgcactaac actttcacgc
    36841 ttttctgagc ctaaatcggc aataagttta ttattaccat cataaaccaa actagagtta
    36901 acggatttta aagcttgatc ggttaatttc ggagaacgac taacgtagta tgcaaatagg
    36961 agcccaccta ttacaatagt taagataata aatcccatta taatgccaaa agcatacttc
    37021 aatagtttga taacagattc ctttttaatt gtaatcaccg cctaataatt tttcttcaat
    37081 aatatctaag taagggactt gaggaaaggc actctccttt acgaaaaatc cattttttct
    37141 gatataatca ataggcattg atttattgcc tttatcaatc tgataaaatg aaattaactc
    37201 atgagcaggg agtagatagg tttccttaag tgtggaaaaa tgaagcaaga caaagcaaat
    37261 ccctttttgc tgtaatacat ttgccatgtg ctctatttgg tgagcatgaa aatttttcat
    37321 aggcatagca gttttctgcc gggtttcttt ggcttcaaag tcaatatagt aacctttgta
    37381 aacaccagaa taatcagtag tagaagcttg tctaaaataa gcttctacaa tcttagcacg
    37441 actacgttta ggataatcta ccttgacgat ttgtactggc gttggtttct tatgaatgac
    37501 agctaattcg tgagacaaat agtagtcatt ggttgcattg atagcagctt caaatgacat
    37561 accacggttg gcaaaatcga ttttcttttt ctttgatttt gtaactgttg ttttccgaat
    37621 aagctgatgt ggatagttaa ccatttttct ccttgcatta caaataaaat gctaagataa
    37681 gcaaaactat tataccataa aattaggatg gacaacaaat gaagtcaact attcttgtaa
    37741 caggttacaa aaattttgaa ttagggattt tccaggataa ggatcctcgg attactatta
    37801 taaaaaaggc tattgataag gattttagac gcttcttgga aaatggggca gactggttta
    37861 tttttatggg aaatttaggg tttgagtact gggctttgga agttgctctt gatttacaaa
    37921 aagaatatga ttttcaaatt gcgacaattt ttacctttga gaaccatggc caaaattgga
    37981 atgaggctaa taaagctaag ttggcactct ttaagcaagt tgactttgtt aaatatactt
    38041 tcccaagcta tgaaaacccg ggtcaattta aacaatacaa tcattttctc attaacaaca
    38101 cacaaggtgc atatcttttt tatgattctg aaaatgaaac taatttaaaa tttttgctag
    38161 aaatgatgaa aaaaaaggaa gcttatgata tttctttcct aacttttgat cgcttaaatg
    38221 aaatatatga ggaatgatcg ttttttcgga tatgagaacg ttttacgctt gatttttcca
    38281 tttaatttag gtattattta ctttgatata aaggatagga cttatattaa aacgatttga
    38341 ctggtttgga gaatagaatg gcaagtatta tttatagccc aaaagatatt tttgagcaag
    38401 attttaaagt tagtatgcgg ggatatgaca agaaagaagt cgatgtattt ctggatgatg
    38461 tcatcaaaga ttacgaaaat tatcttgagc aaattgagaa gctccaaatg gaaaatagac
    38521 gtctacaaca agctttagac aaaaaagaaa gggaagcttc aaatgtaaga aattcaggga
    38581 cagctatgta taatcaaaaa ccgattgccc aatctgcaac caattttgat attttaaaac
    38641 gcattagccg ccttgagaaa gaagtttttg gaagacaaat tagagaataa agatttctca
    38701 aatatgcaat ttttggataa tcgcgtagta ttgatatact atgaggaaag tccatgctag
    38761 cactggctgt gatgccagta gtgtttgtgc taggcgaaaa aataagccta gggagatagc
    38821 tagctatctt acggcaagca aaagggctaa gtctttggat atgcctgaat agctttgaaa
    38881 gtgccacagt gacgtagttt ctagggaaat ctagaaagtg gaacgcggta aacccctcaa
    38941 gctagcaacc caaactttgg taggggcatg ggatagttgg aatgagaaca atctatcctg
    39001 actgctttgc agtagacaga tgattatcga aagaaacaat tcctagttgt ttctggaaca
    39061 aaacatggct tatagaaaat tgcataatag gttagtggct gagataacat ctcagccaat
    39121 ttttaactgt gatgaatacg atagtgtaaa agaggaaaaa tgaaagaatc attcaaatta
    39181 atagcaactg ctgctgctgg tttagaagcc attgttggta gagaaatcag aaacctagga
    39241 attgactgcc aagttgagaa cggtagagtc cgtttccacg gagatatcaa aacaatcatt
    39301 gaaacgaacc tatggctacg agctgccgat cgtataaaga ttattgtagg agagtttcca
    39361 gcacgtactt ttgaggaact ttttcagggt gtatatggcc ttgactggga aaattattta
    39421 ccgttagggg caaaatttcc tattgctaaa gcaaagtgtg tcaagtcaaa attgcacaat
    39481 gaacctagtg tacaagccat ttctaaaaaa gctgttgcta agaaacttca aaaagtcttt
    39541 caccgtcctg aaggagttcc cttacaagaa aatggagctg aatttaaaat agaagtttct
    39601 attttaaaag ataaagcgac tgtcatgatt gatacgactg gatcaagtct cttcaaacgt
    39661 ggctaccgtg cagaaaaagg tggtgcacca attaaagaaa acatggcagc agctattatt
    39721 caattatcta attggttccc tgataagcca ttgattgatc caacttgtgg ttcaggtact
    39781 ttttgtattg aagctgctat gattggaatg aatatagctc ctggttttaa tcgtgatttt
    39841 gcctttgagg cgtggccatg ggtagatcaa agtcaagtac aaaaggttcg tgatgaagct
    39901 gagagtaaag caaactatga tattgattta gatatttcag gttttgattt agacggtcgt
    39961 atggtagaaa ttgctagaaa aaatgcagaa gaagcaggac ttggagatgt tatcaaattg
    40021 aaacaaatgc gtcttcaaga tttaaaaaca gataaaatta acggtgttat tatctccaac
    40081 ccaccatatg gtgaacgact cctggatgac aaagcagtgg acattttgta taatgagatg
    40141 ggacaaacat ttgcgccttt aaaaacttgg agcaagttta tcctaactag tgacgaagga
    40201 tttgaaaaaa aatacggctc acaagctgat aaaaaacgta agctttacaa tggtacttta
    40261 aaagttgatt tgtaccaata ttacggtgaa cgtgtgagac gtcaagtaaa ataacgggga
    40321 gaactcatgt cagaagatca aaaacaccca ttttttgaac cagaaaaaga aactgatggt
    40381 cttgaattta aagatgctaa agaaatgaca gtagaagagg ctgttagaaa agattctgaa
    40441 attaaggctg gtattaccga agaagatagt attcttgata aatacatcaa acaacatcgt
    40501 gatgaagtag cttcacaaaa atttgagaca aaatcaagcg attttgcaaa cctcgatacg
    40561 gcatctttag atgattttat caagaaacag cgcgaagaat tatcagcgat gttagctgca
    40621 gaagagcttt ctaaaaagtt ggataattca gtctctcaag aacaagatac agaagctaat
    40681 gctgtctcac ctaaagaaga atcttcacaa gaacaagaaa atagtgtaac cccagttccc
    40741 cctctgatta cggaagcaga accaactgca acagaaccag attcgactat tgctgattca
    40801 gaggagtata aatcctcttc taggaagcgt ggaggtatcg taggaacatt gattgctctt
    40861 attttacttc taatagtagc aatctttggt tacaactatt tcaagaataa taattcgaca
    40921 aattctcaaa ctgcaacaag tcaaagttct agttctaagg ctacgacgac ttcatcagaa
    40981 gaagataaaa aagctagtca gaacttagat aatttcaaca aagcttatgc taactttttt
    41041 gttgatgata agaaaaccca actcaaaaat agcgaatttg ataaattgtc agaattagaa
    41101 aagaaagttg atgctttaaa aggaactaaa tactacggta aagtaaaagt taaatttgat
    41161 agtctaaaac gtcaaattga tgctgttaaa gctgttaacg acaagtttaa gtcgcctgct
    41221 gtagttgatg gtaagaagag tgaaaaatta gaagtaaaag atggagcgaa ttttgactct
    41281 ttagatagta aaactcttaa tactggtaat gctagtctag atagtttact tcactccatc
    41341 gtttcaactg gtcgtaatca ggtaaaacaa tccgaagaac aggcttcgtc taataaggtt
    41401 tcagacactc aaacaactga acaaccaaac gtaactaacg gtcaatcttc ctcatcagct
    41461 gctacaataa ataatcaggc agctggcact gcatcaggta atttggaacg taaccgtagc
    41521 cgagtaccgt ataataatgc tgctattgcg gatacaggaa atcctgcctg gatatttaac
    41581 ccaggagtat tagagaaaat cgtggccact tcacaggcgc gaggctattt ctctggaaat
    41641 aactatattc ttgaacctgt aaatattatc aatggtaatg gttattacaa tatgtttaaa
    41701 tcagatggca cttatttgtt ctctatcaat gccaagacag gttactttgt aggtaacgct
    41761 cctggaagag ctgatagttt agattattaa tataaagaac aaaaccagtt ttttgtctac
    41821 accctaaaag ttagaaaatc agtctaacga ttagggtgtt ttttgtattc gcatttagat
    41881 gtctacgact gctatgagga tattagggaa aaattaaaag gattttgctc tctatgatac
    41941 agaacaaaat cctttgttta aattaatagg acaacttttg gaattccaaa agttgacttt
    42001 tattttttat tagatcagac aatgtggcgc tcaaaagcat tgtcagaaat tccttgggaa
    42061 agaattaatt tcgcccattc ttgagcagag tggagactgt ggtctttata atttccacaa
    42121 gattcaatgg ttgttccagg aacgtcttcc caagtgaccc caccagcaat agcctctaga
    42181 ctagatttga tgactttagc aatttcagta gcatcctgtt ttccccacat aatcatgtga
    42241 aaaccagttc tacaaccaaa aggagaacaa tcaatcaaac cattaattcg ttggcggatt
    42301 aacttcgcca gaaggtgctc gatagtatgt aaaccagcag tatcaatagc attttcatta
    42361 ggctgaataa ggcggatatc aaagttagta atgatgtctc cgaccggtcc aacttcttct
    42421 gaaattaaac gaacgtaggg tgctttaacg atggtgtgat ctaattcaaa actctctacg
    42481 acaacttctt ttgtcataag ttttctcctt ttgtattaca ttctagagac taaaggagat
    42541 gttgaaaatt tctcctaatc cctttaaata acattctaat catatcataa atgtctataa
    42601 ttttaaattg tgaaaacgcc ttttttatga taaacttaaa agtggacttt ctatatgaaa
    42661 tctagaaaaa aattgaggta taaacatgtt taatattata ttagcaatgg tttgtgcact
    42721 cattggtttg attattggtt atgtggctat ctcaatgaaa atgaaatcat caaaagaagc
    42781 tgcagaacta actcttttaa atgcagaaca agatgccgtt gatttacgtg gtaaagcaga
    42841 aattgaagca gaacacatcc gtaaagcagc ggaacgtgaa agtaaagctc accaaaaaga
    42901 attactttta gaagcaaaag aagaggctag aaaatatcgt gaagagatcg aaaaggaatt
    42961 taaatctgat agacaagagt tgaaacagat ggaggcacgt ttaacagatc gtgcatcatc
    43021 tcttgatcgt aaagatgaaa atctatctaa caaagaaaaa atgttagata gcaaagaaca
    43081 aagtctaacc gataaatcta gacatattaa tgagcgcgaa caggaaattg ctactttaga
    43141 aacaaaaaaa gtagaagaat tgtcacgcat cgcagagctt agtcaagaag aagctaaaga
    43201 tatcatttta gctgataccg aaaaagattt agctcatgat attgcgacac gtattaaaga
    43261 agcagaacgt gaagtaaaag aacgatcaaa taagatcgca aaagacttgc tagcccaagc
    43321 tatgcaacgt ttagctggtg aatatgttac tgaacaaaca attacaactg ttcatctccc
    43381 tgatgataat atgaagggac gtatcattgg tcgtgaagga cgtaatattc gtactctgga
    43441 aagtttgaca ggtattgatg ttatcataga tgatacgcca gaagtcgttg tcttatcagg
    43501 tttcgacccc attcgtcgtg aaattgctcg tatgacttta gaatctttga ttcaagatgg
    43561 acgtatccat ccagctcgta ttgaagagtt ggttgaaaaa aatcgtttgg aaatggatca
    43621 acgtatccgt gagtatggtg aggcagcagc ttatgagatt ggtgcaccaa atctgcatcc
    43681 tgatttgata aaaattatgg gacgtttgca gttccgtaca tcatatggtc aaaatgtact
    43741 tcgtcattca gttgaagttg gtaaacttgc aggtattttg gctggagaac taggagaaaa
    43801 tgttgattta gctcgtcggg ctggattcct ccatgatatg gggaaagcta ttgatcgcga
    43861 agttgaaggc agtcacgttg aaattggtat ggaatttgct cgtaagtaca aagagcatcc
    43921 gattgttgtt aatactattg caagccacca tggtgatgta gaacctgaca gcgttattgc
    43981 agtaattgtt gctgctgcag acgctcttag ttcagcacga ccaggtgcgc gtaatgagtc
    44041 aatggaaaat tatatcaaac gtctgcgtga tttagaagaa attgctaatg gctttgaggg
    44101 agtgcaaaat gcctttgcac ttcaagccgg acgtgaaatt cgtatcatgg tacaacctgg
    44161 taaagtttct gatgatcaag tagttattat gtcgcataag gtacgtgaaa aaatcgaaca
    44221 aaacttggat tatccaggta atattaaggt aacagttatt cgtgaaatgc gagctgttga
    44281 ttttgcaaaa tagtcaaaag gagagcttgt tagctctcct tttgttatag agttgacatt
    44341 tgtcatattt tgaaatgaca aaaatatcta aaaagtttag ttctgatttt ttatactatg
    44401 attatagtaa aggagaacca aatggcaaaa gatataatta aggttgatca tatatttaaa
    44461 agcatcgggc aaaaaacgat tttagaagat atatcattta gtatagcttc taaccaatgt
    44521 gtagcattga ttggacctaa tggtgcaggg aaaacaactc tgatgtcaac tttattaggt
    44581 gatattagta tttcttcagg agcattgacg atttttaatc tgcctgcaca ccacaatagg
    44641 ttaaaatata agatggccat cttaccgcaa gaaaatgtat tgccctcaaa gtttacagtg
    44701 agagaactca ttgatttcca aaggtgttta tttccagaag tgctgccaat gtctttgatt
    44761 ttagactatc tacagtggag tgatgcgcac ttgcaacaat ttacagagac tttatctgga
    44821 gggcaaaaac gacttttagc ttttgtttta actttggttg gtaagcccca attactattt
    44881 ttagatgaac ctacttccgg ataagttatc agactttggt gatagaatca cgtttctaat
    44941 tgttcctatt ttttgtatcg gttacatgtg gattgctatt gaaaatagaa atagtgaaga
    45001 acaaagagaa aaaattgcag agcaaaatca gtatatcaat attttgtcag ctgaaaatga
    45061 acgtaaccgt atagggcgtg atttgcatga tagtttagga catacatttg ctatgatgac
    45121 actaaagaca gaactagcgt taaagttgtt agaaaaaagg aactatgata aggtacaaaa
    45181 agaactatca gaattaaatc atattagtca tcaatcgatg agtgaggtcc gtcaaattgt
    45241 ttctaacctc aagtatcgta cagtagtgga agaaattgat gagttgtacc gcttgtttca
    45301 attatctaac atcaagttaa cagttgttaa taagttagag acgtcacaac taagtccagt
    45361 aacacaatct acaatcacaa tgatattaaa ggaattatcc aataatattg ttaagcatgc
    45421 tgaagctgat agcgtggagt tgagtttagt aaggcaaggt gctactataa atattgagat
    45481 gattgataat ggttgtggtt ttacaaacct tgacggagat gagttgcata gtattcaaga
    45541 gagattaact atcgttgaag ggacactaac gattttgtct cgaagtaaac caactcatat
    45601 tcaggtggta ttgaaagagg tataagatga aattacttgt tgctgaggat cagtccatgc
    45661 tacgagacgc tatgtgccaa ttacttttga tggaagaaag tgtttctact attgatcaag
    45721 ctggaaatgg tgaagaagct attgccattt tatccaataa agccatagat gttgcaatat
    45781 tggatgttga gatgccgata ttatcaggtt tagatgtctt agagtgggtt agaaaatacc
    45841 agaatgttaa ggtcataatt gtaacaacct ttaagcggtc aggttatttc taaagggcta
    45901 ttcgttcaaa tgtagatgct tatgttttga aagaccgctc agtagctgat ttgatgaaaa
    45961 cgattcaaaa agttttatct gggggtaaag aatattcgcc tgaattaatg gaaaacgtta
    46021 tatctaatcc tttgagtgag caagaaataa aaattctttc cctaattgct caagggaaga
    46081 ccagcaaaga aattgcatgc actttatttt tatcgaatgg tacagttcgt aattacacgt
    46141 caaatatttt tgataaatta ggagttaata atcgtttgga ggcacttaaa atagcacgag
    46201 aaaaggattg gctgtgatag tttttttcca gtcgagatgc cataatgtat taagagggat
    46261 atatgtatgc aaaaatttaa agaacgtttt gatacgctta gtgatgcaat tttggccatt
    46321 gctatgacga ttttagtttt ggagataaaa actccggcta cgatgggtga tattggtgac
    46381 tttacacgga acataggttt atttattgtt agctttgttg ttgtttttaa tttttggtac
    46441 gaaagggcac aaaatagcct agatgctcaa aagactaatg atgaaattat agctcttgat
    46501 atcattgaac acctaggtat ttgtttgatt cctctcttta ctaaatttat gatttctttt
    46561 gagaatcata attttgctgt tatggcttat ggactcttaa cgttattagt tggtctcaca
    46621 tcagacatta ttcggattca tttggcaagt tatgatttag tgaccattcc tagtgaattg
    46681 aaagagcggg ttataaaagt catgacaact tttgccattc gttcagttgt ggttcgcttt
    46741 atcattatta tccttgctta ttttctacct gaggtaggga tatttgcata tttagtcata
    46801 ccgctcttta tgttttggcg tcgctctaga gcaggaaaat actatgctaa aaagggcatt
    46861 gaggcaccct cttatatcaa gctttttatc caaattggag agagagttcc tgaggatttt
    46921 actaattcaa gtagtggaat aggtggggta aaaagactct ataaaaaata ataggagacc
    46981 ttgaaaaaaa ggtttctttt tgttaaacta atgagaagaa actttattta gggagcaatt
    47041 tatgaattgc tataaaagtt aatagttaag gtaagaacca gattgttctg gtctttttaa
    47101 cacttaacac ccgatctatt aatttgagga gtgtgtaatg tctgaacgtg gtttgctaat
    47161 tgttttctca ggaccatctg gtgttggtaa ggggacagtt aggcaagaaa ttttttcaac
    47221 ccctgaccat aaatttgatt attccgtttc aatgactact cgtccacaac gcccaggcga
    47281 agttgatgga gttgattatt tcttccgtac acgtgaagag tttgaagcgt tgattaaaga
    47341 aggtcaaatg cttgagtatg cggagtatgt tggtaactac tatggaactc ctttaagtta
    47401 tgtcaacgag actttagaca aagggattga tgtttttctt gaaatagaag ttcagggagc
    47461 gcttcaagtt aaatcgaagg ttccggacgg tgttttcatt ttcttaacac caccagattt
    47521 agaggaatta gaagagcgtt tagtaggacg tgggacagat agtccagaag tgattgccca
    47581 acgtattgaa cgtgccaaag aagaaattgc tttgatgagg gagtatgact atgctgttgt
    47641 caatgatcaa gtgtcattag cagcagaacg tgttaaacgt gtcattgaag cagaacatta
    47701 tcgtgtggat cgtgttattg ggcgttatac caacatggtc aaagaaactg ataaaaaatt
    47761 aagttaaaaa tagaaaagag aattgcctta tgatgttaaa accttctatc gacactttgt
    47821 tggacaaagt tccttcaaaa tattctttag ttattttaca agctaagcgt gctcatgaat
    47881 tggaagctgg tgagaaagcc actcaagatt ttaaatccgt gaaatcaaca cttcgcgctt
    47941 tagaagaaat cgagtcaggt aatgttgtaa ttcacccaga tccatcagca aaacgtgcct
    48001 cagttcgcgc tcgtatcgaa gctgaacgcc ttgctaaaga agaagaagaa cgtaaaatca
    48061 aagagcaaat tgctaaagaa aaagaagacg gcgaaaaaat ttaagcaagt caaaagtgag
    48121 aggttgaaac attctgtatc agcctctttt catattttag agaattaggg agggtttatg
    48181 gcaagaaaat tagcacaggt tattgttgat attcctctga tgcagacaga taaaccattt
    48241 tcgtatgcca ttcctaaaga tttggaagat ttggtacaag taggagcacg cgttcatgtt
    48301 ccttttggaa gagggaatcg tcttttgcaa ggctttgttg tcggtttcag agatgatgat
    48361 gaattagaga ctaaggatat tgcgggggtc ctagattttg aacctgtttt aaatcaggaa
    48421 caactagact tagctgatca gatgcgccat actgtctttt cttataaaat atctattttg
    48481 aaatcaatgt taccaagtct tttgaattcg caatatgata aactcttgtt agcaacggac
    48541 acattaccat cagaagatag agaagatcta tttggtcata aaacggaaat cgtcttttca
    48601 agtttgtctt ctcaagatgc caaaaaagca ggcagattaa ttcaaaaagg tttcatagaa
    48661 gttcaatatt tagccaaaga taagaaaaca ataaaaacag aaaagattta caaaatcaac
    48721 agaactctgt tagaaaagag ccaaatagca gcaagagcga aaaagagact ggagttgaaa
    48781 gaattcctgt tagaaaatcc tcaaccaggg cgcctcacag ctttaaacaa acaattttca
    48841 tctcctgtgg ttaatttttt cagagaagaa ggaatcattg aggtcattga aaaagaagct
    48901 agtagatctg ataattattt taaaggaatc ctaaaaacgg attttttgga tttaaatcag
    48961 gaacaagcta aagtagttaa aatagttgtt gaccgaattg gtaaggatca gaataaaccc
    49021 ttcttgttgg aggggattac agggtctggt aagacagagg tatatcttca tatcattgat
    49081 aacgttttaa agttgggaaa aacagcgatt gttctcgttc cagaaatttc tttaactccc
    49141 cagatgacga atcgttttat ttctcgtttt ggaaagcgag ttgctattat gcactcgggc
    49201 ttgtcagatg gtgaaaaatt tgatgagtgg cgaaagataa aatcaggtca agctaaagtt
    49261 gtcgttggag ctcgttcagc aatttttgct cctttggaaa atatcggagc catcattata
    49321 gatgaagaac atgaatcaac ttataagcaa gaatctaacc caaggtatca tgcgcgcgat
    49381 gtggccttgc tacgtgcaga atatcataaa gctgtgttac taatgggatc agcaactcca
    49441 agtatcgaaa gccgagctag agctagtcgg gatgtttata aatttttaga attaaaacat
    49501 cgtgctaatc ctaaagcacg tattcctcag gttgaaatta ttgattttag aaattttatt
    49561 ggtcaacaag aagttagtaa tttcacacca tatttactag ataaaatccg agatagatta
    49621 gataagaaag agcaagtggt tcttatgctc aatcgtagag gttattctag ttttattatg
    49681 tgtcgagact gcggctacgt tgatcaatgt cctaattgtg acatttctct gactttacat
    49741 atggctacaa agaccatgaa ttgtcattat tgtgggtttg aaaagcctat tccaagaaca
    49801 tgtccaaatt gtaatagtaa aagtatctct tactatggta ctggaaccca aaaagcttat
    49861 gaagaacttt tgaaagttat cccagacgca aagattttac ggatggatgt tgatacgaca
    49921 aggcaaaaag gcggtcatga gtctattttg aagcgttttg gaaatcatga agctgatatt
    49981 ttattgggaa cacagatgat tgctaaggga ttggattttc caaatgttac tttagttgga
    50041 gtactaaatg cagatacttc tctgaattta cctgattttc gatcatcaga gcgaactttt
    50101 caactattaa ctcaagtggc tggacgtgct gggcgtgctg aaaaagaagg agaggttgtc
    50161 attcaaactt ataatcctaa tcattatgct attcaattag cccaaaagca agattttgaa
    50221 gctttttatc aatatgaaat gaatatccgt cgacaacttg gttatcctcc ttattatttt
    50281 acagttggtt taacattatc gcataaagat gaagagtggt taatacgaaa atcttatgaa
    50341 gtattaagtt tattgaagca aggctttagt gataaagtaa agttgttagg tccaacgcca
    50401 aaaccaattg caagaacaca taacctttat cactaccaaa ttattatcaa gtatcgattt
    50461 gaggacaacc ttgaacttgt tttgaatcgc ttattagaca tgactcaaga caaggagaat
    50521 agagatcttc gcttagcaat agaccacgaa ccgcaaaata tgatgtagaa tagtgctatg
    50581 atataattga gaaaagaaag acgaggaaaa agcaatgaca aaattattat tcatgggtac
    50641 ccctgatttt tcagcgacgg ttttaaaagg tattttagca gatggtaaat atgatgtttt
    50701 agctgttgtg acgcaaccag atcgtgctgt aggtcgtaaa aaagaaatta aaatgacccc
    50761 tgtaaaagaa gtggccttgg aaaacaacat tcctgtgtat cagccggaaa aactatctgg
    50821 aagtccagaa ttagagcaac taatgaccct aggtgcagat ggtattgtaa cagctgcttt
    50881 tggtcaattc ttaccaacaa agcttttgga atcggtaggt tttgcaatta atgtacatgc
    50941 ttctctattg ccaaaatatc gtggtggggc accaattcat tatgccatta ttaatggcga
    51001 aaaagaagct ggggttacca tcatggaaat ggttgcaaag atggatgcag gggatatggt
    51061 atcaaaagct tccgttgaga taactgatga agacaatgta gggacaatgt ttgatagact
    51121 cgccgttgtt ggtcgtgacc ttttattaga tacgcttcca ggttatctat caggtgatat
    51181 taagccgata ccacaaaatg aagaagaggt aagtttttct cctaatatta gtccagatga
    51241 agagagaatt gattggaata aatcatcgcg cgacattttt aaccatgttc gagggatgta
    51301 tccatggcca gttgcacata ctcttttgga aggaaatcgt tttaaacttt atgaagtaac
    51361 aatgtcagaa ggaaagggct caccgggtca agtgatcgct aagacaaaga actcattgac
    51421 agtagcaact ggtgacggag ctattgaact caaatcagtt caaccagcag ggaaacctcg
    51481 tatggatata aaagactttt taaatggtgt tggtcgtaat ttagaaatag gagataaatt
    51541 tggcgaatga ttggaaaaag agtgcgcgtg gtttagcttt aatgacttta gaggaagttt
    51601 ttgataaagg agcttattct aatatcgcac ttaataagag tttaaaaaaa tctagactat
    51661 cagataaaga tcgtgcctta gtaacagaaa ttgtctatgg aactgtggct agaaaaataa
    51721 cgctagaatg gtatctatca cactttatag ttgatcgtga taagcttgaa ttatgggtat
    51781 atcatctctt gctactgagt ctttatcaac tactctatct ggacaacatc ccagatcacg
    51841 ctattgttaa tgatgctgtc actattgcta agaatcgagg caataaaaag ggtgctgaaa
    51901 agttaattaa tgccgtttta cgaagagtaa gttcggagac gctccctgaa attgcatcaa
    51961 taaaacgcca aaacaaacgt tactctgttg cttattccat gccagtgtgg ctcgtaaaaa
    52021 aattaattga ccaatatggt gagacaagag ctttagctat tatggagagt ttatttgagc
    52081 gaaataaagc cagtcttcgt gtgactgact tatcgcaaaa acaaactatc aaagaaactt
    52141 taaatgttag agattcacat attgcagaga cagctttagt tgcagactca ggaaattttg
    52201 catcgacatc atttttccaa gatggcctca ttactatcca agatgaatct agtcaattag
    52261 ttgctccaac actcaaagtc tcaggtaatg accaagtatt ggatgcatgt agtgcaccag
    52321 gaggaaaaac aagtcatatc gcctcatatt tgactacagg ggcagtaact gctttagatt
    52381 tgtatgatca taaattggaa ttggttatgg aaaatgcaaa acgcctaggt ctgtcagata
    52441 agataaaaac taaaaagtta gacgcaagta aagctcatga gtattttcct gaggatactt
    52501 ttgacaaaat tttagtagac gcaccttgtt caggtatcgg gctaattcgc cgtaaaccag
    52561 atattaagta caataaagct aatcaagact ttgaagccct ccaagagatt caattgtcta
    52621 tcctatctag cgtttgtcaa acgctacgta aaggtggtat aataacctat agcacttgta
    52681 ccatttttga ggaagaaaat tttcaagtta ttgaaaaatt cttagcaaac caccccaatt
    52741 ttgagcaagt agaactgagt catacacaag aggatattgt aaagagaggc tgtattagta
    52801 taagccctga acaatatcat acagatggct tttttattgg acaagtcaaa cgtatcttgt
    52861 agtgtctagg aggaaactga catcgtcaga aaaaaatata tggaaatatc tcttttaaca
    52921 gatattgggc aacgtcgttc caataatcaa gattttatta atcaatttga aaataaagcc
    52981 ggcgtcccac taatcatctt agcagatggt atgggaggac atcgtgcagg aaatattgct
    53041 agcgagatga ctgtaactga tctaggaagt gattgggcgg agacagattt ttctgagtta
    53101 tcagaaattc gtgattggat gcttgtctct attgaaactg agaatcgcaa aatatatgaa
    53161 ttaggtcagt cagatgatta caaaggtatg ggaactacca ttgaagcagt tgctattgta
    53221 ggagataata ttattttcgc acatgtagga gattcacgta tcggtatcgt tcgtcaagga
    53281 gagtatcatt tattgacgag tgatcattct cttgttaatg aattggtaaa agctggccaa
    53341 ttaacagaag aagaagctgc tagtcatcct caaaaaaata ttattacgca atcaattggt
    53401 caagccaatc cagtcgaacc tgatttagga gttcatctac ttgaagaagg tgactacctt
    53461 gtagttaata gtgatggact aactaatatg ttgagtaatg ccgatattgc tacagtacta
    53521 acacaagaaa aaaacttaga tgataagaat caagatttaa tcactctagc aaaccataga
    53581 ggaggtcttg ataatatcac tgtggcacta gtttacgttg aaagtgaggc ggtttaatga
    53641 ttcagattgg caaattattt gctggtcgtt atcggatcct taaatcaatc ggacgaggtg
    53701 gaatggcgga tgtttatcta gctcgagatc taattttaga taatgaagag gtcgcaataa
    53761 aagtattgcg aaccaattat caaacagatc agattgcggt tgctcgcttc cagagagaag
    53821 cacgagcaat ggctgagctg acacatccta atattgtcgc tattcgtgat ataggtgaag
    53881 aagacggaca gcagttctta gtaatggaat atgttgatgg attcgattta aaaaagtata
    53941 ttcaagacaa tgcaccccta tcaaataatg aagttgttcg aatcatgaat gaagtccttt
    54001 cagccatgtc actggcacat cagaaaggta tcgtacaccg tgatttaaaa cctcaaaata
    54061 ttttattaac taaaaaaggg actgtaaaag ttacagattt cggtattgcg gtagcgtttg
    54121 ctgaaactag tttaacgcaa acgaattcta tgttaggtag tgttcattat ttatcgccag
    54181 agcaggcgcg tggttcaaaa gcgactgttc agagtgatat ttatgccatg ggaatcatgc
    54241 tttttgaaat gttcactggc catattcctt atgatgggga tagtgcagtt actattgccc
    54301 tacagcactt ccaaaaacca ctaccatcaa tccttgcaga aaataaaagc gttccacaag
    54361 ctttggaaaa cattgtcatt aaagcaacag ctaaaaagct aacagatcgc tataagacaa
    54421 cttatgagat gggacgtgat ttatcaactg ctctaagctc gacacgtcac cgtgaaccaa
    54481 aacttgtttt taatgatact gagagtacta agacactacc aaaagtaaca tctacagtat
    54541 catctttaac gacagaacaa ctgttgcgta atcaaaaaca ggccaaaaca acagaaaaaa
    54601 taactcctga ttctgcgagt aacgataaaa cgaagtctaa aaagaaggct tctcatcgac
    54661 ttcttggtac aatcatgaaa ttattctttg cactctgtgt tgtaggaata attgtatttg
    54721 cttataaaat tttagtaagt cctacgacta ttcgtgttcc agatgttagc aacaaaacag
    54781 tagctcaagc aaaaatgaca ttggaaaatt ctggattgaa agtaggagct attcgtaata
    54841 ttgagagtga tagtgtttca gaaggccttg tcgtaaaaac agaccctgct gcaggacgtt
    54901 ctcgtagaga aggggctaaa gttaatcttt atattgccac tccaaacaag agtttcactc
    54961 taggtaatta taaggaacat aattacaaag atatcctcaa agatcttcaa ggcaaaggtg
    55021 ttaaaaaatc tttaatcaag gttaggcgta aaattaataa tgattatact actggtacaa
    55081 tcttagcaca gtcgcttcca gaaggaacat cttttaatcc tgatggtaat aagaaattaa
    55141 cacttacggt tgctgttaat gatccaatga ttatgcctga tgtaacaggt atgacagttg
    55201 gagaagtaat agaaaccttg acagacctcg gattagatgc agataatctt gtcttctatc
    55261 aaatgcaaaa tggcgtttat caagcagtag taacacctcc atcttcaagt aagatagcaa
    55321 gtcaggatcc ttattacgga ggagaagttg gattaaggag aggagataaa gttaagctgt
    55381 atcttcttgg ttctaaaaca acaaataact catcatctac gccaatagat tcttcggctt
    55441 caagctcaac aggaacaact acctcggatt ctgtaagctc atcaacggat gctagcactt
    55501 ctgatagtag tagcacttct acaagtagtt caacactacc aagtgatagt acaactaata
    55561 caggaacagc taacaatcca ttaactcaat aaaattaaaa agaagatgtt aaagtcttct
    55621 ttttaatttg ctatggtaga gaccatgcct agaaaggttc tgccaatatg ccaaattttt
    55681 ctctcaataa tggtgcaaag tatagggctt tttgataaaa tacgtcatat agagtaatgg
    55741 aaggattatt atgaaaaaat ttcagttttt tctacttgtt gaagccgttg tcttagttat
    55801 gggattaatg aaaattttat cagatgactg gacaagtttt atttttattc tcgctttgat
    55861 tcttttagcc ttaagatttt ataacaacga tagtcgtcat aattttctgt taacgacaag
    55921 tttgttatta ctatttttaa tctttatgct taacccttac attattgcag ccgttgtttt
    55981 tgcagttttg tatgttctaa tcaatcattt ctcgcaagtt aagaaaaaaa atcgttatgc
    56041 tttgattcaa tttaagaacc atcaattaga tgttaagaca acacgaaacc aatggttagg
    56101 tactgatcag cacgaaagtg acttttatgc ttttgaagat attaatatta taagaatctc
    56161 tgggactgat acaattgatt taacaaatgt tattgtatct gggcaggata atgttattat
    56221 aattcaaaaa gtttttggtg atactaaagt attggttcct ttagacgttg ccgtaaaggc
    56281 tgatattagt tctgtttacg gtagtgtaca gtactttgat tttgaggagt atgatctaag
    56341 aaatgaatct ataaaattgt cacaagagga agaatattac cttttgaaac gtgtcaaatt
    56401 agttgtaaac actatcgctg ggaaagttga ggttagtcgt aaatgaaaaa gcaccactac
    56461 tttttagctt tcttctatgg aagtgtcatt atatttgcca tttgttttgt cattattgat
    56521 agtctgggtg ttaatttggt gcacctctat caaacgtctc gtttgtggct gattgaacaa
    56581 ttgattttct ctattttctt tctatcttta gcggtaacta tccttttatt gctaacttgg
    56641 tttttattag atgataatag taaacgtcaa attaaccata atttacgtcg cattttaaat
    56701 aaccaatcaa taaatgttac cgatgatggt actgaaatat cgaccaatat tcaacggctc
    56761 tctaaaaaaa tgaatttaat gacagcaagt ctacaatcta aggaaaatag tcgtatttta
    56821 aaaagccaag aaattgtgaa acaagaacgt aagcgtattg cacgtgattt acacgacaca
    56881 gttagtcagg atttgttcgc agcttccatg gttttgtcag ggattgcaca gaatgtttct
    56941 cagctagacg ttgatcaggt tggctcgcaa ttattagcag tagaggaaat gcttcagcat
    57001 gcacaaaatg atttgagaat tctgctactt catttgaggc cagttgaatt agaaaataag
    57061 accctatctg aaggattcag aatgatattg aaagaattga ccgataagag cgacattgaa
    57121 gttgtttatc atgaatcaat tctaacctta cccaaaaaga ttgaagacaa tatttttaga
    57181 attggtcaag aatttatcag taatacttta aagcattcac aagccagtcg tctcgaagtt
    57241 taccttaatc aaacagagaa tgaattgcag ttaaaaatga ttgataatgg tattggattt
    57301 gatatggatt ctgtctatga tttaagttat gggttaaaaa atattgaaga tcgagtagaa
    57361 gatttagcag gaaatttaca gcttctcagt cagcctggta aaggagttgc tatggatata
    57421 agattacctt tagtaaatca gagtgaggat aaaaatggat aaaataaaaa ttgtacttgt
    57481 tgatgaccat gagatggttc gtctcggttt aaaaagtttt ttaaacttac aagctgatgt
    57541 tgaagtaata ggtgaggcct caaatggttt agaaggcata aaaaaagcac tggagttacg
    57601 tccagacgtt gttgttatgg acttagtaat gcctgaaatg gacggtgtag aagcaacctt
    57661 agcattattg aaggattggc cagaagctgc tattttagtc ttaacatctt acttggacaa
    57721 tgaaaaaatt taccctgtta ttgaagcagg tgccaaaggc tatatgttaa agacctcaag
    57781 tgcagcagag attcttaatg ccattcgtaa agttgcaagg ggagaacaag ctattgaaaa
    57841 tgaagtagat aagaaaatta aggctcatga taaatgtcca gctttacatg agggcttaac
    57901 agcaagggaa cgtgatatcc ttaatttatt agccaaaggt tatgacaatc aaaggattgc
    57961 tgatgaatta tttatctcgt taaagactgt taaaactcat gtttctaata ttttgggaaa
    58021 actaaatgta gcagatcgaa ctcaagcggt tgtctatgct ttccaacacc atttggtacc
    58081 acaagatgat gaataagtga tataatagag gcactatgtt aatgaaagtg gttagtcatg
    58141 gacgcaaaaa ctaaatataa agcaaaaaaa ataaaggcag tgttttttga tattgatgac
    58201 acgcttcgtg ttaaagatac aggttatatg ccaccatcaa tcttaaaagt ttttaaagct
    58261 ttgaaagata aaggaattgt tgtaggtatt gcttcaggaa gggcacgata tggcgttcca
    58321 aaagaagttc aagatttaaa tgccgattat tgtgtaaaat taaatggagc ttatgttaag
    58381 gacaaggata agaatatcat cttccatcgt cctattcctg ctgaatatgt agagcaatat
    58441 aagaaatggg cagacactgt tggtattaag tacggtttag ctggtcgtca tgaggcagtt
    58501 ttatcagatc gtgatgattt ggtaaatgat gcgattgata ttgtgtattc tgatctcgaa
    58561 gttaatccag atttcaataa agaacatgat atttatcaga tgtggacgtt tgaagataaa
    58621 ggagatagcc ttcatctacc tgaaccgtta gatgaacatc ttcgcttgat acgttggcat
    58681 gatcattctt cagatgttgt gttaaaagga acatcgaagg ctttaggtgt ttctaaagtt
    58741 gttgaacatt tgggcttaaa gccagagaat atcttggttt tcggagatga gctcaatgat
    58801 ttagaactct ttgattatgc tggactagct gtggcgatgg gagtgtcaca cccagaagct
    58861 caaaaaaaag cagattttat cacaaaaaaa gtagaagaag atggcatctt gtatgccctg
    58921 gaggaactag gtttgataga aaaagaatta acatttccac aagtagatat tgaaaataca
    58981 gaaggtcctg tggctgtcat taaaacaaat cacggggata tgactgtaaa actcttcccg
    59041 gatcatgctc ctaaaacggt agcaaacttt attggtttag ctaaacaagg ttattatgat
    59101 ggtattatct tccatcgtat tattccagat tttatgattc agggtggtga tccaactggt
    59161 acaggtatgg gtggtgaatc tatctatggg gaaagttttg aagacgaatt ttccgaggaa
    59221 ttatataatg ttcgcggagc tctttcaatg gctaatgctg gaccaaatac gaatggcagc
    59281 caatttttca tcgtccaaaa tacaaaaatt ccttatgcga aaaaggaatt agagcgaggt
    59341 ggttggccaa caccaattgc tgagttgtat gctggacaag gtggtacacc gcatctagat
    59401 cgtcgccaca gtgtatttgg acaattagta aaccaaagtt cttttgaggt acttgatgag
    59461 attgcggcag tagagactgg ttctcaagac aaaccattgg aagatgttgt tattttaacg
    59521 attgaggtta aagaataaaa tgaagattgg cgataagtta aagggaacag tcactggcat
    59581 taggccttat ggagcctttg tttctcttga ggatggtagg actggactta tccatatttc
    59641 tgaaattaaa acaggttaca ttgataatat ttatgatgtt cttagtgtag gtgatgaagt
    59701 ctatgttcag gttatagatg tagatgaatt tacacaaaaa gcaagtttat cgcttcggac
    59761 ccttgaagaa gaaagacatc atattcagca tcgtcatcga ttttctaaca atcgtcttaa
    59821 gataggtttc aaaccacttg aggaaaattt acctagttgg gttgaggaag gtctagccta
    59881 cttgaataat aaataaacaa aaaacctaga tttgattaat gacaagtcta ggttttaaat
    59941 atttaaaagt agcagtgaat attatacttg ctaaaaatag cttgataatc gagtagatcc
    60001 tctgggtgta gtgcagcgat ctcttccatt tcatattgac gattaagtaa ttgatatttg
    60061 ttttgtccaa attggtggaa aggtaagagt tgcacttgac ggatatctag gcgatcaaat
    60121 aaacaagcaa attcttcagc atcttctaat gaatcgttaa aattaggtat gacaggaata
    60181 cgtagcacaa tagttttacc gttggcaaaa gcataatgaa tgtttttgat gattgaagca
    60241 ttcttaacca tcgtcttttc ttggtgtttg aggctattgt aatgttttaa atcggtgtaa
    60301 ataaaatcaa cgtattggat aagatctatg aattgttcat gtctagtata agcagttgtt
    60361 tcaatagccg tgtggattcc cagtgattta gcacgtttta aaattgcctt ggcaaattca
    60421 aattgtgcaa atatttcacc gccagatagc gttatgcccc caccagattc ttcgtaaaaa
    60481 tcaatatctt ttaaaacctc ttcaataata tcatcaaccg atttttcttc gcccacgatg
    60541 acgctttcat tagtaatggc atctctcatt gtttctggaa ccattttttg cgactcggga
    60601 ttagcacacc aaggacatct aagaggacaa ccctttaaga aaacggttgt tcgaatacct
    60661 ggaccatcgt gaatactaaa atgttgaatg ttgaatacaa tacctttttc atttgtcata
    60721 ttattttcct caatttcatt ttaagtatac cttttatacg taacaaagtc aattacgaat
    60781 gaaagtttat tgaacttgtt taataaataa aaaatgataa aatagaaagg aggtgtttca
    60841 tgaatagatt agaaaatatt attagtttag tatctcagta ccaaaaaata gacgttaata
    60901 cactctcgga gttgttacaa gtttctaaag taacgatacg taaagattta gataaactag
    60961 aaggcaaagg gcttttgcat agagagcacg gttatgcagt tttaaacagt ggggatgatc
    61021 ttaatgttag gttatctttt aaccataaaa ctaaaaaaga aattgctgcc ttagctgcga
    61081 atatggtgtc tgacaatgac actatcttaa tagaatcagg ctctacttgt gctcttttag
    61141 cggaaaatat ctgtcagaca aagcgaaatg tcactatttt gaccaactct tgctttattg
    61201 ctaactattt acgagaatat gattcatgcc aaattgtctt acttggtggt gagtatcaat
    61261 caagttcaca ggttacggta ggtcctctct tgaaaaagat gatttcactt tttcatgtct
    61321 cgttagcttt tgttgggact gatggttttg atccaaaact aggatttatg ggtagagatt
    61381 taatgcgttc tgaagttgca caagagatgg cgaatgctgc cgatgaagtc attattttaa
    61441 cagattctag taagtttaat cagactgcat tggtggaaca gcttccgttg tcaactgtat
    61501 cacaagttat tacagataaa catccaaatt cggaaattgc taatttgttc caagaagcag
    61561 agattaccat tcaatcttta ttacaagtgc cataaaagag gctatttttc ttacattttg
    61621 cacatttgtg gcgataatag ataaagtgtc atgtcaaatt gcacagatgt aacctttttg
    61681 ttaaactgaa gcaaatgctg aaaggttatg agtatgaaat tagagcgtca aaaattgtta
    61741 gctaaagtag cttaccttta ctatatggaa gggaaaagcc aatctgagat tgccaatgaa
    61801 ttaggaattt atcgtacaac aataagtcgt atgttagcca aagcgagaga agaaggtctt
    61861 gttagaatcg aaatttcaga ttttaatcca gaaatttttc agttagaatc ctattttaaa
    61921 agtaaatatc accttaaaga tattgagatt gtttcttcgc gaaaagatag cgatacttca
    61981 gagatagaga aggatttagc acatgtagct gcggctatga ttcgaaaaaa aattaaagag
    62041 aatgacaagg tggggattgc ttgggggagg acacttagta aagtagttga agcaatgcgt
    62101 cctcatccgg tttctcaagt ttcttttgtt cctttagctg gaggtccaag tcatattaat
    62161 gcacgctatc acgttaatac tctggtttac gaaatgtcaa ggcgtttcca aggaagttgt
    62221 actttcatta atgcaactct agttcaagaa aacgctaatt tagcgaaagg aatattaact
    62281 tcaaaatatt ttgaggggct aatggataat tgggaaaaac ttgatgtggc aattgtgggc
    62341 gttggtggta aacctaaaag caatgaacaa cagtggcttg atcttttaaa tcaagatgat
    62401 tttcaatgct tggatgaaga agcagctgta ggtgaaatta cttgtcgttt ttttaatcat
    62461 tcaggtgatc ctgtgaatca acatttagct aaaaggacaa taggaattac cttagaacaa
    62521 ctacagaaag ttcctaatag aatagcggtt gctcatggca attataaggc tgcggcactc
    62581 ttagccgttt taaaaaaggg gtatattaat catttagtta ctgatttttc cacagcctta
    62641 aatattttaa gattagataa agacaccttt gtggatacta tatatcaaaa gagctaggaa
    62701 taaatcctag ctcttttgat aatgtttcta ttaccaagtg aattgttata gaataagcac
    62761 aaaggtgcgc tattgtcata aagggcacaa ggtctattga cgacgattaa gggacatgtt
    62821 acatttgttt tgcgaaagaa aattagaacc ttttgtaaga aagtttaaac ttataaaaaa
    62881 tcattaaagc ggaggtgctt atggaaatca tagtcgcaga ccaaattatt atggggctca
    62941 ttttgaatgc tggggatgct aaacaacata tctatcaagc cttaaaactt gcaaaagaag
    63001 gaaattttgc agaaagtaaa attgaaatag aactagcaga tagtgcactc ctagaagctc
    63061 ataatcttca aacacaattc cttgcacaag aagcaggagg gacaaggaca gatatctcag
    63121 cactctttat tcattctcag gatcatttga tgacaagtat tactgagatt aacttgatta
    63181 aagaaattat tgatttacgc caggaattac atattcaaaa ataagtaaag gggatcaaat
    63241 atgataaaaa ttggtttatt ctgtgcagca ggtttttcaa caggtatgtt agtaaacaat
    63301 atgaaaatcg cagcggataa agaagggata gaggcacata ttgaagctta ttcacaagga
    63361 aaaattgcag attatgctaa agatttagat gttgcattac ttggtccaca agttagctat
    63421 actctagata aatcaaaatc aatctgtgat gaatacggtg taccgatagc agttattcca
    63481 atggcagatt atggtatgtt ggatggtgta aaagttttga aacttgcttt gagtttattg
    63541 gaaacaagaa aagaggcttg atatgtcaaa gtttgatagt cagaaaataa ttactccgat
    63601 tatgaagttt gtcaatatgc gagggattat tgcactcaaa gatggcatgc tagcaattct
    63661 accactaaca gttgttggga gtctcttttt aatattaggg cagcttccat ttaaaggtct
    63721 taatcaagcc atagctaatg tttttggacc agaatggaca gaaccattta tgcaagttta
    63781 ttcaggaact tttgcgatta tgggcttgat ttcctgtttt gcaattgctt atgcttatgc
    63841 taaaaatagt agtgtagaac cgttacctgc tggcgtttta tcactttcgt ctttctttat
    63901 tttaatgaaa tcatcttata ttcctgtaaa aggagaagca atagcggatg ctatttctaa
    63961 ggtttggttt ggcggtcagg ggattattgg agcaattatt attgggttag tagttggtgc
    64021 tatctataca tggtttatcc aacatcatat tgttataaaa atgccagagc aagtaccaca
    64081 ggcaatagca aaacaatttg aagctatgat tccagctttt gttatctttc ttttatcgat
    64141 gattgtttat ttgattgcta aggtaacaac tggaggtacc tttattgaga tgatttatga
    64201 tatcattcaa gtaccgttgc aaggcttaac aggctcactt tatggagcaa ttgggattgc
    64261 tttctttatt tcattcttat ggtggtttgg tgtccatggc caatctgtgg tgaatggtat
    64321 tgtgactgct ttgttactat ccaatttaga tgccaataag tctttattag cagcaaatcg
    64381 cttaacatta gataatggcg ctcacattgt aacacagcag tttttggata gtttcttgat
    64441 tttatcgggc tcaggaataa cttttggact agttattgcg atgctttttg cagcaaaatc
    64501 aaaacaatac aaggcacttg ggaaagtggc cgcttttccc gcgattttca atgttaacga
    64561 gccaatcgtc tttggctttc caatcgtaat gaatcctgtg atgtttctgc cttttatttt
    64621 agtaccagtt ttggcagctt taattgttta tggagctatt gcggttggtt ttatgcaacc
    64681 attctcaggt gttactttac catggagtac tccagcaatt atttctggat tcatggtagg
    64741 tggttggcaa ggtgcactcg tccaaatagt cattttagct atctcaactg ctgtttactt
    64801 cccattcttt aaaatccaag ataatattac ttacaaaaat gaatgtgaaa tggaaagggt
    64861 atagctagca gacaagaggt tgaggtgtgt aatgcacctt aacctttttc taaaaatgca
    64921 catttgtgca actttagatg tagcattcta ttctttattt tcttcttgtt attttaggat
    64981 ttaggtgcta ttatatttac gaagaaaaac tttttagttt cgaaagaaac aagcggaggc
    65041 tgctatgacc aaacaattac tcactcaaaa atatacaaat gatgttcaac aaaattctca
    65101 aaagcatttt ggctatttga cagagcgcat gtattcttat cgtgataagg ttcttgataa
    65161 gaaaccattc atagatgcag aacgtgctat cttggtaact gaagcctatc aaaaacatca
    65221 ggaaaaacct aatgttttaa agagagctta catgttgcaa aatattttgg aaaaaatgac
    65281 aatctatatt gacgatgaga caatgattgt cggtaaccaa gcttcatctg ataaagatgc
    65341 tcctattttt ccagaataca ctctagaatt tgttgtaaat gaactagacc tttttgaaaa
    65401 acgagatggt gacgtctttt acattacaga agaaaccaaa gaacaaatta gaaatattgc
    65461 acctttctgg gaaaataata atttacgggc tcgtgcgggc gttatgcttc cagaagaagt
    65521 tcaagtttat atggaaacag gcttctttgg gatggaaggt aagatgaatt ctggggatgc
    65581 tcacttagct gttaactatc agaagttact agaagaaggg cttattggct ttgaaaaaaa
    65641 agcaagaaaa gctaaagctg acttagattt aacaaagcct gagagtattg acaaatatca
    65701 tttctatgat tctattttaa ttacaataga agctgttaaa acttatgcag agcgttttgc
    65761 tatcctagca aaaaaacaag ctaaaacagc aaatgctaag agacgtcagg aattgttaga
    65821 tatcgctagt atttgtgaac gagtgccata ttacccagct aaaacgtttg cagaagctgt
    65881 tcaatcggtt tggtttattc agtgtattct tcaaatagaa tcaaacggtc attctctatc
    65941 ttatggacgc tttgatcaat acatgtatcc atatgttaaa gctgacttag aagctggacg
    66001 tgagactgag gattcgattg ttgagcgcct aactaaccta tggattaaaa caataactat
    66061 taataaggtt cgtagccaag ctcatacgtt ttcgtctgct ggtagtcctc tttaccaaaa
    66121 tgtaacgatt ggaggacaaa cccgacataa ggaagatgct gttaatcctt tatcatttct
    66181 agtactaaaa tcagtcgctc agacacactt accacagcct aatctaacgg ttcgttatca
    66241 tgctaaccta gacaagtcat tcatgaatga agctattgaa gttatgaaat taggctttgg
    66301 tatgccagct tttaataatg atgaaattat tattccatca tttattaaaa aaggagtttc
    66361 cgaagaagat gcctatgatt attcagcgat tggttgtgtt gaaacagcag ttcctgggaa
    66421 atggggttat cgttgtaccg gcatgagcta tatcaacttc cctaaagtgt tattaattac
    66481 aatgaataat ggcattgacc cggcttctgg gaagagattt gcgccttctt atgggcattt
    66541 cacccagatg acctcatata aagaacttaa agaagcttgg gacaaaacac ttcgttactt
    66601 aactcgaatg agcgttattg ttgagaatgc tattgatatt agtctcgaaa gagaagtacc
    66661 tgatattctt tgttcagctt taactgataa ttgtattgga cgaggtaagc acttaaaaga
    66721 aggtggtgca gtatatgact acatatctgg tctacaagtt ggaatcgcta atctttcaga
    66781 ctcacttgct gcgcttaaaa aattagtatt tgaagaaaaa cgcctaacca cattagaagt
    66841 atggcaagca ttacagagtg attacgcagg tccgcgtggt gaagaaattc gtcaaatgtt
    66901 gatcaatgaa gcaccaaaat atggaaatga tgatgattac gcggatagtt tagtacgtga
    66961 gtgttatgat gtttatgttg aagaaattgc taaatatccc aacacacgct atggacgtgg
    67021 accaatcggt ggtattcgct attcaggaac gtcctctatt tcagcaaatg taggccaagg
    67081 acgaggtact ttagcaactc cagatggtag gcatgcagga actccattag ctgaaggttg
    67141 ttcaccatct cataatatgg acaaaaaggg acctacctct gttttgaaat cagtttcgaa
    67201 gttaccaact gatgagattg tcggcggtgt actccttaac caaaaagtca accctcaaac
    67261 cttggcaaaa gaagaagata aacaaaaatt aattgcctta cttagaacat tcttcaatcg
    67321 ccttcatggt taccatatcc agtataatgt tgtctcacgt gagactttga ttgatgctca
    67381 gaagcaccct gaaaaacacc gcgatttgat tgtgcgtgta gcaggttatt ctgcattctt
    67441 taatgtccta tctaaggcaa ctcaagatga tattattgca cgtacagaac atgctcttta
    67501 ggagattatt atggaatttt tacttgacac tttaaattta gaagctataa aaaaatggca
    67561 tcatatactg ccactagcag gtgtaacttc aaatcccacc attgctaaga aagaaggaga
    67621 tatacatttt ttccaacgca ttcgtgatgt tcgagaaatt attggacgtg aagcaagttt
    67681 gcatgttcaa gtagtggcca aagattatca agggatactg gatgatgctg ctaaaatacg
    67741 ccaagaaacg gatgatgaca tttatatcaa agtcccagtt acgccagatg gattagcagc
    67801 aattaaaacg ttaaaagcag aaggttataa tattacagcc acagcaatat acaccagtat
    67861 gcaaggtctt ttggctatat cagctggagc agactaccta gcaccttact ttaaccgaat
    67921 ggagaacctt gatattgatg cgacacaagt tattaaggaa ttagcgcaag caatagagcg
    67981 gacaggttct tctagcaaaa ttttagcggc tagttttaaa aacgcttcac aagtaacgaa
    68041 ggccttatca cagggtgctc aatcaatcac cgcgggacca gatatttttg aatcagtctt
    68101 tgcgatgcca tctattgcaa aagctgttaa cgactttgca gatgattgga aagccagtca
    68161 acactcagaa catatttaac agagaacgcc aacttttgag gctgtatctt taacataaaa
    68221 ttttttaaag aagaaagagg ttgagaatga aagttttcgc aagtccatcg cgctatatac
    68281 aaggaaagga tgctttattc caaagtattg aacatatcaa atctttaggt cagactccac
    68341 ttatcctttg tgatgatgtt gtttataata tcgttggaga aagtttttta tcttatctac
    68401 aagacgattt gttaccacat cgtgtctcct ttaacggaga agcatctgat aatgaaatca
    68461 atcgtgtcgt tgctgttgcg aaagaaaaga atagtgacct tattattgga cttggtggtg
    68521 gaaaaactat tgactctgca aaggcaattg cagataaagt taatttacca gtggtcattg
    68581 caccaacagt agcttcaaca gatgccccaa catcagcgtt atcagtaatc tatacagatg
    68641 aaggagcttt tgaaaaatat attttttatt caaagaatcc agacttagtt ttggtcgata
    68701 ctcaagtaat tgctcaagcg ccaaaacgtt tactagcttc tgggattgca gatggcttag
    68761 ctacttgggt tgaagcgaga gcagtcttac aaaaaaatgg aatagcgatg gctggcggta
    68821 gacagacttt agcgggcgtg gcaattgccc aagcatgtga gcgtacttta tttaatgata
    68881 gtttacaagc tttagcggct tgtgatgcca aagtggttac aaaagcttta gaaaatgtta
    68941 ttgaagctaa taccttactt agtggtttag gttttgaaag tgcaggatta gctgcagctc
    69001 atgctattca taatggtttt actgccttga gtggagatat tcatcactta acccatggtg
    69061 aaaaagttgc ctatggaacg ttgacgcaac ttttcctcga aaatagacca aaggaagaaa
    69121 ttgatcgcta cattaatctt taccaagcga ttggaatgcc aactacactt gcagaactcc
    69181 atttgggtga tgcaacatat gaagaacttt taaaagttgg tcagcaagca acaattgaag
    69241 gtgaaacaat acatgaaatg ccattcaaaa ttagtgcaga ggatgtcgct gcagccctgt
    69301 tagcagttga ccgctatgtt tcaaatcatc aataaaaaca atagaaccaa gtgaaattgc
    69361 ttggttctat ttgtcgtttt aatattttaa ctaaataggg cttactagta tttaaagaga
    69421 tataaactaa aaaagatcag acaacatatc taatcttttt tagttatcgt ttactgaaga
    69481 ttaaagttat tcaaaatcat acaggctagt tgataagtaa cgttcgccat tatcagggag
    69541 aagagctaaa acctttttcc cttttcctac ttgtttggcc acttcaatag cagcataaat
    69601 agcagctgaa gcagaaattc ctgcgaggaa accttctagt ccgccgatga tacgtcctgt
    69661 ttcaatagca tcgtcagatg taacgcgaat gatatgatca taagaatctg tagccaatgt
    69721 ttctgggatg aaacctgcag aaatcccttg tattttatga ggcccaggtt gctcaccaga
    69781 aaggattgca gattcatcag cttctacagc ataaatagcg acatcaggat tgactttttt
    69841 taatgcacga gagacaccag taattgtccc accagttccc acacctgcga taaaagcatc
    69901 taaacctttt tcaccaaaag tctcgataat ttccctacca gtcgtttttt cgtggatttc
    69961 tggattggct tgattattaa attgcaaggg taaccaagca ttttgctcta cagaaatttc
    70021 ttttgcctta gcaatagctc ccttcatccc ttcactacca ggtgttagta ctaattgagc
    70081 accataggct tgaatgattt tacgacgttc aattgacatg gtttctggca taacaataat
    70141 aacattatac cctttagctt ttcctaccca agccaaaccg atacctgtat taccagatgt
    70201 aggttctacg attgtatctc cggctttaag aatccctttt tgctctgcat cttcaatcat
    70261 agctagagca atacgatctt tgacagatga acctgggtta aaggattcta acttaacgta
    70321 cacatctgca gctccttctg gaacaatatg atgtaattga ataatgggtg tatttccgat
    70381 taagtctgta atagaattat aaatttttga cataagctat acctcttcat gatattggtt
    70441 aagaatatta taaaactcac attgattaaa gtaaaatatc aattctttat caagtgatag
    70501 ctattattta tcggtttcat ccttaaaggg aatttcaaca atttgactcc cttcttcagt
    70561 taacaaagct tttccttggt agaactctgt caaattagtt attgtgtgtt caaattcctt
    70621 agggtcaacg tatattttac aggttacagc ttctaaaaat tctgtttcaa actcctgaag
    70681 gtggtgctct tttaggaagt tgtcaaaagt ttgatattga ggataggtta attgaatacg
    70741 aataccaatt tgttctttaa cttctactac ctcgatctct ttgatggcat tagctacgct
    70801 accagagtag gcacgaataa gcccccctgt gcctaatttg atacctccaa aataccgtgt
    70861 aacgacggca acaacatttg tcaggccttg cttttcaagt acggttagca tagggatacc
    70921 tgctgtacca ctaggttctc catcatcact agatcgtttt atatcccctt tttcgccaat
    70981 aaccatagct gaacacgaat gattggcttt gtagtgcgct ttcttgattt gcgtaatata
    71041 attacgcccc tcctcttcgg actctactcg ctttagatga caaataaata gcgatttttt
    71101 aatttcttct tcgacaattc cgtctgattt tatcgtttta tagttcatag agaaatttta
    71161 caaaaattct tcaaatttag caaaaaaatc cgaatgaaat aatatggaaa attatttagg
    71221 tagactttgg acaaaagcac aattatctga gcaacttaga aaaattgcaa ttaacttgcc
    71281 atcttttata aagaaaggaa gcgactatat ttgtactcga tgctctagtt ctgttgccaa
    71341 aaattgccaa ttaccaactg gaaattatta ttgtagagaa tgtatagttt ttggaagagt
    71401 tactagtaat gaaaatttat attatttccc acaaaaaaca ttttctaaaa cgaattcttt
    71461 aaaatggaaa gggggactga caccttatca aaatgaggtt tcagaagagt tgttgaaagg
    71521 tatttcaagt aaggaaaatc ttcttgttca tgctgtgaca ggggctggta aaacagaaat
    71581 gatataccac tctatcgcca aagtaattga tactggagga agcgtttgta ttgccagtcc
    71641 tagaattgat gtttgtttag aattgcataa gcgactttca aacgatttta gatgtgccat
    71701 aacattaatg catggagaat ctccttctta tcagagatcc cctttaacta ttgccacaac
    71761 acatcaattg ttaaaatttt atcatgcttt tgatttgcta attgttgatg aagttgatgc
    71821 ttttccatat gtcgacaacc cgatactata ccatggtgta aaacaggcct taaaggaaaa
    71881 cggaacaagt atttttctga ctgcaacttc aacgactgag ttagaaagaa aagttgcaag
    71941 aaaagaacta aaaaaattac atctagctag gagatttcat gccaatccgt tagtgatacc
    72001 cgagatggtt tgggtaagtg gtatccagaa aagcttgcag acacaaaaac tacctcccaa
    72061 attatatcag ctgataaata aacagcgaca aacgagatat cctttgttat tatttttccc
    72121 ccatatttca gaaggtcagg tattcaccga aatgttaaga caagcctttc ctatggaaaa
    72181 aattggtttt gtttcaagta agagcacatc taggttaaag ttagtgcaag attttcgaga
    72241 taataaattg tctatcctag tgtcaactac cattctagag agaggagtaa ctttccctag
    72301 cgtcgacgtt tttgttatac aagcaaatca tcatttgttt acgaaaagtt ccttggtaca
    72361 aatttcagga agagttggaa gagcattaga aaggcccgaa gggctccttt acttccttca
    72421 tgatgggaaa agtaaatcga tgcaccaagc tataaaggag attaaaaaca tgaatcatat
    72481 aggaggattt taatgacttg tttgttatgt catgaaatag atttatcgca attaactttt
    72541 gtggaactta tgcttctaaa accaaagcaa aacgtaattt gtcaaacgtg caagggtagt
    72601 tttgaagcac ttagtaggga gatgggttgc caaacatgtt gcaagcaaat tccccaaaaa
    72661 caatgtcaag attgtattta ttggggtaaa aaaggtatag aggtaaatca tttttccctt
    72721 tatagataca atgaagcaat gaaaaaatat ttttctcttt ttaaatttca aggggattat
    72781 ttgttgaaag atgtttttac aaaagaaata aaagctgctc taaaaaggta taagggctac
    72841 actatagtgc cagttccctt gagtcatgaa ggataccaaa ataggcaatt taatcaagtg
    72901 attgcttttc tacaatcggc aaatatacct tataaaaata ttctttctaa aaaagataga
    72961 ggtaagcaat cggctaataa taaagaagaa agactcaaac aagttcagca atttacatta
    73021 aaaaatgagg ctgagttaag ggataacctt ttaattgttg atgatattta tacaacaggc
    73081 gcaacaatag cacaaatcag gaaactatta gaagaaaaag gtataaaaaa tataaaaagt
    73141 ttttcattag cacgctaaaa tcttgcaaat tttcatgaaa gcggttataa taagaatata
    73201 gaaagcgtta acaacgcaag aaagaggtac tcatatgatt aaatacagta ttcgtggtga
    73261 aaacatcgaa gtaacagaag ctattcgtga gtatgttgag accaaactct ctaaagttga
    73321 aaaatacttt aatgaagaac aagaattaga tactagagtc aacctcaaag tatacagaga
    73381 gaagaccgca aaagttgaag taacaatctt aatagattct attactttac gagcagaaga
    73441 tgtttcacaa gatatgtatg ggtcaattga cttagttgtt gataaaattg agcgtcaaat
    73501 tcgtaaaaat aaaactaaaa ttgcgaaaaa atatcgtgaa aaaattccag caagtcaagt
    73561 tttcacaaca gaatttgagg ctgagccaga tgaagaagca gtaagccaaa gaattgtacg
    73621 tactaagaat gttaatctaa aaccaatgga tgttgaggaa gcattgttgc aaatggaact
    73681 tttaggtcat gatttcttta tctatacaga tgcagaagat aatacgacaa atgtccttta
    73741 caagcgtgaa gatggcgaac taggtttaat tgaagctaaa ggaaattaac tttgtaacat
    73801 aatttaaaaa atctcagagt tttttctgag atttttttaa aacctattga caacaaataa
    73861 aaaattatag tatactaaat gagttcttaa gcgaacctaa tattgtatta tcattttata
    73921 aacaact
//
LOCUS       DBJYSH010000002        63472 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.002, whole genome shotgun sequence.
ACCESSION   DBJYSH010000002 DBJYSH010000000
VERSION     DBJYSH010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 63472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63472
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.002"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            complement(15..1355)
                     /locus_tag="ACUBV3_000071"
     CDS             complement(15..1355)
                     /locus_tag="ACUBV3_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HHU3846611.1"
                     /translation="MTKKGWRIMPAIITTAILGFSGILIETSMNVTFPLLMKEFGVNP
                     AVIQWVTTGNLLAVAVTVPLSAFMIKNLSERQIFTLANVLFLFGVLIDSFAPNLAILL
                     VGRVLQGVGTGLALPLLFHIILTQIPMERRGLMMGVAAMVTLLAPAVGPTYGGVISGM
                     LGWKMIFMLLAPILIISTFIGLASIPKRQVRINDKLNFPAFVSLGIGLATLLLAIEKM
                     SIFYLLVAIVSFVIFYYLNKQLEFLNLNVFKDKDFSILLYGVLAFQMIPLALSFLLPN
                     LLQLVLHQTSTKAGLFMFPGAIAVVFLSPFAGYLLDKIGAFKPIMIGISLSLIGLIGT
                     AIFIPAKFVVVLLAFDILTKIGMGIGASNMVTTALTKLKPAQSADGNSILNTLQQFAG
                     AFATAVASQIFTIGQVAIPKNGAIIGSQFAVLFVIVVVILAIVGLTYLRKRKAI"
     gene            1647..2318
                     /locus_tag="ACUBV3_000072"
     CDS             1647..2318
                     /locus_tag="ACUBV3_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3846612.1"
                     /translation="MAFASNESENNFFITLKTYFNYLFSIQIITDISTLNHADFDGSF
                     AFHDIETSIPHLVIDSNYLAISQTNSKTEANDIKTFSELSKTMTEFYYMLNFDLFNHL
                     PYRFRLHNKDGQTIYSNHKPEDPFDIYPEEEYPIDKWVQNSLIEKKAKELHLLLPGAS
                     QDYILVQSYKRLENDSGQLVGYIEHVHNIKPLLEGYLKESGQAIVGWSDVTSGASISN
                     DDFEL"
     gene            complement(2389..4389)
                     /locus_tag="ACUBV3_000073"
     CDS             complement(2389..4389)
                     /locus_tag="ACUBV3_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HHU3846613.1"
                     /translation="MQHVNHSSFDKASKAGFIIALGIVYGDIGTSPLYTMQSLVENQG
                     GISSVTESFILGSISLIIWTLTLITTIKYVLVALKADNHHEGGIFSLYTLVRKMTPWL
                     IVPAVIGGATLLSDGALTPAVTVTSAVEGLKVVPSLQHIFQNQSNVIFATLFILLLLF
                     AIQRFGTGVIGKLFGPIMFIWFAFLGISGLLNSFAHPEVFKAINPYYGLKLLFSPENH
                     KGIFILGSIFLATTGAEALYSDLGHVGRGNIHVSWPFVKVAIILSYCGQGAWILANKN
                     AGNELNPFFASIPSQFTMHVVILATLAAIIASQALISGSFTLVSEAMRLKIFPQFRST
                     YPGDNIGQTYIPVINWFLFAITTSIVLLFKTSAHMEAAYGLAITITMLMTTILLSFFL
                     IQKGVKRGLVLLMMIFFGILEGIFFLASAVKFMHGGYVVVIIAVAIIFIMTIWYKGSK
                     IVSRYVKLLDLKDYIGQLDKLRHDHRYPIYHTNVVYLTNRMEGDMIDKSIMYSILDKR
                     PKKAQVYWFVNIKVTDEPYTAEYKVDMMGTDFIVKVELYLGFKMRQTVSRYLRTIVEE
                     LLESGRLPKQGKTYSVRPDSKVGDFRFIVLDERFSSSQNLKPGERFVMLMKSSVKHWT
                     ATPIRWFGLQFSEVTTEVVPLIFTANRGLPIKEKIELTTTGD"
     gene            complement(4524..5285)
                     /locus_tag="ACUBV3_000074"
                     /pseudo
     CDS             complement(4524..5285)
                     /locus_tag="ACUBV3_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649058.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
     gene            complement(5347..6339)
                     /gene="pta"
                     /locus_tag="ACUBV3_000075"
     CDS             complement(5347..6339)
                     /gene="pta"
                     /locus_tag="ACUBV3_000075"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - pyruvate decarboxylation to
                     acetyl-CoA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HHU3846614.1"
                     /translation="MRSLFGGLREKVASDYVKIVFPEGSDERVLRAAARLKFEGLVDP
                     VILGKADEVHDSLARLGFVDQDYSVIDPEQYEKFEEMKEAFVEIRKGKATMEDADRLL
                     KDVNYFGVMLVKLGLADGMVSGAIHSTADTVRPALQIIKTKPGISRTSGVFLMNRENT
                     QERYIFADCAINIDPNAQELAEIAVNTADTAKIFDINPKIAMLSFSTKGSAKAPQAEK
                     VQEAAKIAKDLSPELAVDGELQFDAAFVPETAEIKAPNSDVAGKANVFIFPDLQSGNI
                     GYKIAQRLGMFEAIGPILQGLNAPVNDLSRGSSAEDIYKLAIITAAQAIDEKSK"
     gene            complement(6365..7249)
                     /locus_tag="ACUBV3_000076"
     CDS             complement(6365..7249)
                     /locus_tag="ACUBV3_000076"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000667470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HHU3846615.1"
                     /translation="MKFEYIAEERCKVKTLLKSHDVSRGLLAKIKYRGGKIFVNGEEQ
                     NAIFLLEIGDVVTIDIPDEPSHETLEPVPHDLDIVYEDDHFLILNKPFGFASIPSSIH
                     SNTIANFIKHYYVSNNYANQQVHIVTRLDRDTSGLMLFAKHGYAHARLDKQLQAKAIE
                     KRYYALVSGSGDLADSGDIIAPIARDVDSIITRRVHESGKYAHTSYQVVARYGDVRLV
                     DIKLHTGRTHQIRVHFAHIGFPLLGDDLYGGRMDLGINRQALHCHSLSFYDPFMGKIN
                     KQTLDLTDDFDSVIMELH"
     gene            complement(7246..8082)
                     /locus_tag="ACUBV3_000077"
     CDS             complement(7246..8082)
                     /locus_tag="ACUBV3_000077"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HHU3846616.1"
                     /translation="MTQMNFTDRATRVAIIANGKYQSKRVASKLFAAFKHDPDFYLSK
                     KDPDIVISIGGDGMLLSAFHMYEKQLDKVRFVGVHTGHLGFYTDYRDFEVDTLINNLK
                     NDKGEQISYPILKVTITLEDGRVIRARALNESTIKRIEKTMVADVVINQVVFERFRGD
                     GILVSTPTGSTAYNKSLGGAVLHPTIEALQLTEISSLNNRVYRTLGSSVIIPKKDAIE
                     IVPKRVGVYTISIDNKTVHYKNVTKIEYSIDEKSINFVSTPSHTSFWERVNDAFIGEP
                     EH"
     gene            complement(8057..8728)
                     /locus_tag="ACUBV3_000078"
     CDS             complement(8057..8728)
                     /locus_tag="ACUBV3_000078"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HHU3846617.1"
                     /translation="MSMDWETFLDPYIQTVGELKIKLRGIRKQFRKQNRHSPIEFVTG
                     RVKSVESIQEKMVLRGISEENLAQDLQDIAGLRIMVQFVDDVDEVLALLRKRHDMTVV
                     QERDYITHMKSSGYRSYHVVVEYPVDTIDGQKKVLAEIQIRTLAMNFWATIEHSLNYK
                     YKGDFPEEIKQRLEKTAKIALELDEEMRKIREDIREAQLLFDPLNRKLSDGVGNSDDT
                     DEFYR"
     gene            8838..9410
                     /locus_tag="ACUBV3_000079"
     CDS             8838..9410
                     /locus_tag="ACUBV3_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HHU3846618.1"
                     /translation="MTHLEIEYKTLLNKDEFNRLTSLFSHVQPITQTNYYFDTETFEM
                     KAHRMSLRIRTLPNRAELTLKIPREVGNLEHNHDLTLEEAKYIVKNGQFPEDTEIASL
                     ILEKGVDPTKLAVFGQLTTTRREMETSIGLMALDSNIYADIKDYELELEVKQPKQGKR
                     DFDQFLKENNINFKYAKSKVARFSATLKNN"
     gene            9587..10561
                     /locus_tag="ACUBV3_000080"
     CDS             9587..10561
                     /locus_tag="ACUBV3_000080"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HHU3846619.1"
                     /translation="MAEQYADKQIKLFSLTANREIAEKISQASGIPLGKMSSRQFSDG
                     EIMINIEETVRGDDIYIIQSTSFPVNDNLWELLIMIDACKRASANTVNIVVPYFGYSR
                     QDRIAASREPITAKLVANMLVKAGVDRVLTLDLHAVQVQGFFDIPVDNLFTVPLFAEH
                     YNQLGLSGEDVVVVSPKNSGIKRARSLAEYLDSPIAIIDYAQDDSEREEGYIIGEVEG
                     KKAIIIDDILNTGKTFAEAAKILERGGATEIYAVASHGLFAGGAADILESAPIREIIV
                     TDSVLSKERIPSNIKYLTASHLIADAIIRIHERKPLSPLFSYRSDKKD"
     gene            10565..11680
                     /locus_tag="ACUBV3_000081"
     CDS             10565..11680
                     /locus_tag="ACUBV3_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HHU3846620.1"
                     /translation="MIYLDNAATTALTPSVIEKMTNVMTSNYGNPSSIHTFGRQANQL
                     LRECRQIIAEYLNVNSREIIFTSGGTESNNTAIKGYALANQLKGKHIITSEIEHHSVL
                     HTMTYLSERFGFDITYLKPNHGQITAKDVQEALRDDTIMVSLMFANNETGDFLPIQEI
                     GQLLRNHQAVFHVDAVQVFSKMELDPHSLGIDFLAASAHKFHGPKGVGILYCAPHHFD
                     SLLHGGDQEEKRRASTENIIGIAGMSQALTDATTNTLKNWTHISQLRTTFLDAISDLD
                     FYLNNGQDCLPHVLNIGFPRQNNGLLLTQLDLAGFAVSTGSACTAGTVEPSHVLTSLY
                     GANSPRLNESIRISFSELNTQEEILELAKTLRKIIGD"
     gene            11682..12029
                     /locus_tag="ACUBV3_000082"
     CDS             11682..12029
                     /locus_tag="ACUBV3_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HHU3846621.1"
                     /translation="MAFEKEINLPDCNYIYTISPNIKKYTLRDTTFTQNRLGNYELER
                     LLETVPNSGDGFPLKITINKELSGFKLLITDKSGLRIVNIFKSTDHKILQDKFYFLMD
                     SLVERNIFTKTAK"
     gene            12184..12822
                     /locus_tag="ACUBV3_000083"
     CDS             12184..12822
                     /locus_tag="ACUBV3_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HHU3846622.1"
                     /translation="MIMDKSIPKATAKRLSLYYRIFKRFNTDGIEKASSKQIADALGI
                     DSATVRRDFSYFGELGRRGFGYDVKKLMNFFAEILNDHSTTNVMLVGCGNIGRALLHY
                     RFHDRNKMQISMAFDLDSNDLVGKTTEDGIPVYGISTINDHLIDSDIETAILTVPSTE
                     AQEVADILVKAGIKGILSFSPVHLTLPKDIIVQYVDLTSELQTLLYFMNQQR"
     gene            complement(12835..13515)
                     /gene="radC"
                     /locus_tag="ACUBV3_000084"
     CDS             complement(12835..13515)
                     /gene="radC"
                     /locus_tag="ACUBV3_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HHU3846623.1"
                     /translation="MYHIELKKEALLPRERLVDLGADRLSNQELLAILLRTGIKEKPV
                     LEISTQILENISSLADFGQLSLQELQSIKGIGQVKSVEIKAMLELAKRIHKAEYDRKE
                     QILSSEQLARKMMLELGDKKQEHLVAIYMDTQNRIIEQRTIFIGTVRRSVAEPREILH
                     YACKNMATSLIIIHNHPSGSPNPSESDLSFTKKIKRSCDHLGIVCLDHIIVGKNKYYS
                     FREEADIL"
     gene            complement(13611..14744)
                     /locus_tag="ACUBV3_000085"
     CDS             complement(13611..14744)
                     /locus_tag="ACUBV3_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000667240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HHU3846624.1"
                     /translation="MKFEKRQVYYVVITFAICYAIQAYWGAVSNILTTLHKAIFPFLM
                     GAGIAYIINIVMSVYERLYIKLFKGSRLLMAIKRSVSMILSYATFIGLIVWLFSIVIP
                     DLISSLSSLLVIDTGALAKLVNNLNENKQISEALNYMGTDKDLVSTLSGYSQQILKQV
                     LSVLTNLLTSVSSIAATLLNVFVSFIFSIYVLANKEQLGRQFNLLIDTYLGSTGKTFH
                     YVRHILHQRFHGFFVSQTLEAMILGSLTVIGMLIFQFPYALTVGVLVAFTALIPVVGA
                     YIGVTIGFILIATESLTEAFLFVLFLILLQQFEGNVIYPKVVGGSIGLPSMWVLMAIT
                     IGGALWGILGMLLAVPVAATIYQIVKDHIIKRQTLRNRARTYR"
     gene            complement(14835..16274)
                     /locus_tag="ACUBV3_000086"
     CDS             complement(14835..16274)
                     /locus_tag="ACUBV3_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000989488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HHU3846625.1"
                     /translation="MMVKQVFPKGFLWGGATAANQCEGAYNVDGRGLANVDVVPTGED
                     RFAIISGQKKMFDFEEGYFYPAKESIDFYHHYKEDLALLAEMGFKTYRMSIAWTRIFP
                     KGDELYPNEAGLRFYENIFKECRKYGIEPLVTITHFDCPIYLIKHYGGWRSRKMIGFY
                     ERLVRALFTRFKGLVKYWLTFNEINMILHAPFMGAGLYFEDGENQEQIKYQAAHHELV
                     ASAIAVKIAHEVDPNSQIGCMLAAGQYYPNTCHPQDYWASMQKNRENYFFIDVQARGK
                     YPNYAKKYFEHLGISIQMTAEDLALLRDYTVDFISFSYYSSRVASGNPTVSEQVQENI
                     FASLKNPYLKSSEWGWQIDPLGLRITLNAIWDRYQKPMFIVENGLGAVDIPDENGYVE
                     DDYRIDYLRQHIAAMRDAIYVDGVNLIGYTTWGCIDLVSAGTGEMEKRYGFIYVDRNN
                     KGEGTLKRYKKKSFYWYKKVIASNGSQIE"
     gene            complement(16434..17048)
                     /locus_tag="ACUBV3_000087"
     CDS             complement(16434..17048)
                     /locus_tag="ACUBV3_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HHU3846626.1"
                     /translation="MLEVIDKALRDYQMKREQFFEINNQTVQEGAIVFTGDSIVEFFP
                     LKKHLGRDYPLVNRGVAGSDSYWLLENLRTQVWELLPSKVFILIGTNDIGLGHSQSEI
                     IANITDIIAEIRAESYMTEINILSVLPVSEEDDYIERVKVRNNQAIKALNKTLSVISG
                     INYIELYDLLVDEKGQLASSFTKDGLHLTDQAYAKISETIKLHL"
     gene            complement(17149..17970)
                     /locus_tag="ACUBV3_000088"
     CDS             complement(17149..17970)
                     /locus_tag="ACUBV3_000088"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HHU3846627.1"
                     /translation="MIKLIATDMDGTFLRSDKTYDKARFSSLLTLMEKYDIKFVAASG
                     NLYDQLLLNFLEYPNRIAYVAENGGRVIDQDGTLLKETYLSNDTVAAVLSYLYQNYPE
                     TLISLSGEKRSYLERRTPINRRTELEYYMPNFIYKDHLLPLDDDRYFQMTLWVNENLV
                     SEMLLDISEHFKNHHIRLTSSGFGCIDVLPADVNKADGIAILLEKWGLKQDQVMVFGD
                     GGNDVEMLRAANISYAMSNAPEEIKAIAKYQTVSNDQDGVLETIENFLHKMSDNE"
     gene            complement(18090..19655)
                     /locus_tag="ACUBV3_000089"
     CDS             complement(18090..19655)
                     /locus_tag="ACUBV3_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006740157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HHU3846628.1"
                     /translation="MKKVITIEPAKQVTHKVELPRFTKRRVAGYARVSTDHEDQTTSY
                     EAQMTYYTDYINSRSDWEFVKMYSDEGISGTNTKKRLGFQEMVEDALDGKIDLILTKS
                     VSRFARNTVDSLSTVRKLKEAGVEIYFEKENIWTFDSKGELLITIMSSLAQEESRSIS
                     ENVTWGRRRQLAEGQVTFSYSQVLGFKKSDTGGFEIDHEEAKIVRYIFHQVLLGNNPN
                     KIARELTAQGIPTPQGKRQWSYGTVKRMLRNEKYKGDALLQKSFTTDFLTKSTKLNEG
                     ELPQYYVENNHEAIIKREVFDLVQVELDKLERKRQTSNIFTGRLFCGDCGSAFGSKVW
                     HSTSKYKRTIYQCNAKYKGEHKCQTPHVTEEEIKGWFLSAINQLLSNRDEIIANTELL
                     IDMAKDTSSLEARLDDLEHHLETIRQDIEDLVDRNARKAQNQDLYQEQYNTLVTAYQE
                     KQKELQETRSVLEEQKSKQLSLDGFIQNFKEQDDLITDFNQELWQTSVERLDIKEDKK
                     IFLTFKNGLRIDL"
     gene            complement(19658..20866)
                     /locus_tag="ACUBV3_000090"
     CDS             complement(19658..20866)
                     /locus_tag="ACUBV3_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000809551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HHU3846629.1"
                     /translation="MKQIKTIQAQKVTAIKRLKVAAYTRVSHTSLLQSLSNQVSHYSQ
                     LIQANPEWDYVGVYSDSAISGRSQAYRRDFQQLLEDCRKGKIDLILTKSISRFGRNTV
                     ELLETVRELKRLGISVRFEKEKIDTLTAEGELLLTLLASMAQEESQSISQNIRWRVKK
                     RFEEGKPYIPQDIFGYRWNGDEYVIEPHEASIVRQVFEWYMEGLSAPKIAKRLDDRGE
                     RTRLGNRFTKRVIYNMFDQEAYCGRLILQKTFRDQFGSRSILNDGQMAKYIVENAHEA
                     IVTPEYFQLVNQEKKRRARRRVSKHDALAKLQGKVYCEHCGLDMVLTLETKFNQEKRV
                     RYYCRTRDAKGVETCLGRTVTEEQLFQAFGESINTEDIHHISFNSVTNEAKAIYRNGE
                     EKHVIIQKGR"
     gene            complement(20923..21084)
                     /locus_tag="ACUBV3_000091"
     CDS             complement(20923..21084)
                     /locus_tag="ACUBV3_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HHU3846630.1"
                     /translation="MTEQDFQQELTYQLTMAQAKQLLSQGLISEAIFQEFKAKMLEKY
                     EPFMSQLVA"
     gene            complement(21081..21482)
                     /locus_tag="ACUBV3_000092"
     CDS             complement(21081..21482)
                     /locus_tag="ACUBV3_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-70"
                     /protein_id="HHU3846631.1"
                     /translation="MTPEQKAAIRYLREHGLGYKAIGVKLSLSSNTIKSFCRREVIEA
                     GEKTDELLPDYCHACGRVLTHIDGKKKKRFCGTPCRQTWWNSHLEEVNRQAYTEHVCL
                     ACGGEFTSYANPKRKYCSRKCYVTARFGDKK"
     gene            complement(21592..23061)
                     /locus_tag="ACUBV3_000093"
     CDS             complement(21592..23061)
                     /locus_tag="ACUBV3_000093"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000515882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HHU3846632.1"
                     /translation="MGKHLVICGHGQGRTTYDPGAVNSKRGITEAGKVRELARLMSKY
                     SGKNIDYITDQNVYDYKSLAILGKGYDSVTELHFNAFNGTARGTEVLIQSSLTADKED
                     LAILSVLSRHFQNRGIKKVDWLYNANEAKNRGYTYRLVEIAFIDNEEDMTIFENKKEE
                     LAKGLVSAITQEEVKTVVSATPSKQGGQPHASTSPVYHVGDSVRVLGHATHYQTGQAM
                     ASWVKGRTYKILQVKAVNQSRSKRAYLLEGITSWVLEQDVEGTSLGHSEQTYTAQKGD
                     SYWRIARKSGTTVDGLLALNGLKKTDVLKIGQTLKVHKATSSIKAVATSLAQRAVASA
                     LSKVGQKVTVPTNPYGGQCVSLVDKIVQELTDKDMAYTNAIDCLTKAKANGFTVIKDA
                     WGVNPKAGDFYVIKTDSHPYGHIGICITDSDGTSIDGVEQNVDGYSDHNKNGINDQLE
                     IGGGGITRRVKRVWMADGSLYDATGTVKLGKVIGWFRLG"
     gene            complement(23063..23467)
                     /locus_tag="ACUBV3_000094"
     CDS             complement(23063..23467)
                     /locus_tag="ACUBV3_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HHU3846633.1"
                     /translation="MKELLATNKVLFSAIGGLIGSIFGEVDGFFFALMIFIAIDYITG
                     LMAAAVEKRLASNIGFKGIFKKIAILFLVSVGHLIDTEIIKQGGAIRTMVIFFYLSNE
                     GLSILENAVRIGLPIPEKLQALLKQFNEKEGD"
     gene            complement(23485..25341)
                     /locus_tag="ACUBV3_000095"
     CDS             complement(23485..25341)
                     /locus_tag="ACUBV3_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF859 family phage minor structural protein"
                     /protein_id="HHU3846634.1"
                     /translation="MADYGSNNDRGYTLLLRVEETGTSTANNTSTVRVQLWLKNGYTT
                     FGMYDCRASVSINGQTLSWSGRPDMYTAHSSLHLIDKTITVSHDSNGSKTISFSATFT
                     GSGGWSPGTLNTGSQTLRLSDIPRSSSATVSGNMMGKVVTITIKRASSDFTHNITWHF
                     GSLSGTIGTGIATSVTWTPSISQLATQIPNNTSGNGHLTLATIYGDKTIGSMTIPITL
                     NLPTSVVPTLGSISVSESHATAKTILTSTSFAQLVSNPKVTFNQGAGVYGSIIPSTGY
                     RAEVFKFENNQWVQLPNVVTSNNGLLGGINWIGRAKVSAYVTDSRGRQSARKEVEITL
                     LEYFKPIFSFSAVRAGSSMNQVTVTRKLKIAPLTINSVQKNKATLTWEVVDLASGQKV
                     TNAGGAANWTSTTEHTKTDFQAILGGTYDTTKSYNIIGKLADLFYATTFEFTVGPEKV
                     VYGLSPSGMGIGKAWTRGVLDVDGSLPAYFDGEIYMKNKKLLDIFYPVGVIYESTSST
                     SPATIMGGTWERFGNGRVLVGVSENESEFKSVNKSGGSKTHTLTVDEMPSHSHAQYVS
                     ANNGNQAIRRDYSSDGNSSLYPQGNNTGNTGGNKPHNNLQPYVTVYRWRRTA"
     gene            complement(25354..28269)
                     /locus_tag="ACUBV3_000096"
     CDS             complement(25354..28269)
                     /locus_tag="ACUBV3_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HHU3846635.1"
                     /translation="MLYLLDGQTKTAKWNGQPLFETVSATVEEELNGIFQLRLTYPIT
                     DSGVHETLRADELILCPTPDLGNQLFRVKQAKIQDDRVELECSHISDDVMKRQIKPFS
                     ATNTTCQSALMRMVEVCPSDLGLFSFDSDVTERHTYVSDEDLTLYQALMDGKHSILGT
                     WEGELVRDNFQLIVKKHRGNDKGVILTSHHNLKAFEDKGDSEKVITRIYATSTFQIEG
                     SDEDTVLSVVVESPLINQYPYVHEARYENNTLQTEEELRQWAMAKFTHEHIDSISRQI
                     TVEAYQLDGQEVHLGDTVTLKSQKHKVDVKKKAVSYTFDALEEVYLLVTFDDEVSFTT
                     SGSSGNNSLTSAAKTILDVNQSATEHRASKERANFKKVFDRHFERLQTEVEDGIAKAK
                     AEGERSGKKAALDYLATDALEARVAALQKATIDELTVSSSAWMTRLVSQQILSEYVKS
                     LEIEADKVVIPGQHTPVFSLDRDGSLSIDTPLLKVRGDSLATKVDLKTISLTPGPKGD
                     AGADGVGIQLREQYYLVSAQKTGITTTSSGWSKTIPSLTSTLKYLWNYEKTTFTNGST
                     TVTTPIVIGVYGDKGVDGKAGKDGKTLYTWRMYADSDKGDGISAVSTGKRYLGLAVNR
                     ESATPSTNPSDYTWSSFFEGTELGGRNYIDDYAMKAVTFSSVTSEWKKEVVEDTSSVS
                     GVTVKMTCTKAGTAGFHRNFYDLRSRIGATMTFSIDIKCSKSVTLNMGCELGGTKAYE
                     VTTDWQRFVSSWKVSSYQYYSYIFYLKSGSWSVGDVVYLRNVQLEDGNVASAPGPSLN
                     DLIAQIDTKADNGFMKQQLDLLTEKTESLRVDLEARALAKEVADWLKSYKEFEKNNEA
                     VLAQFNQDFIDNTARIAAIEADLKANSLLLNFVNTYLRAGDNGVIIGKKDNSEYIELT
                     SQGMMIKSAGNAVMTVTAGVIKIHHGVFVETLQVGYYRLEAARHNAKHLVCRFIDAK"
     gene            complement(28269..28994)
                     /locus_tag="ACUBV3_000097"
     CDS             complement(28269..28994)
                     /locus_tag="ACUBV3_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HHU3846636.1"
                     /translation="MIKHNELVLNGKGTSSFLFKVLVEDRPSIQVPRSKTQLLDHRGL
                     SGAIVQTNKHREVIEKPYRLYLIGASEKGVNEFSAYLMQEGFWLESERLKLTRLWCYR
                     TDSFDIKQDDHDVYVIDVTFICHPTRFFKSVDRQVLSANGVLKTQGSALAFPSITITG
                     QSVSETSFTVGDQVIRIEKFTEPLVMVNHPDRPSFKTLSGKAVKWSGDFITIDASHPT
                     QSVGVILGSGILSLTFETNWGWV"
     gene            complement(28991..32110)
                     /locus_tag="ACUBV3_000098"
     CDS             complement(28991..32110)
                     /locus_tag="ACUBV3_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000918338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HHU3846637.1"
                     /translation="MAGNIKGITIEIGGDTQPLQNALKGVNKQASEATKELRQIDKAL
                     KFDTGNVTLLTQKQEVLAKQVETTKEKLATLRQAQAQVEAQFKAGNIGADQYRAFQRE
                     VESTQTVLKGYESKLESVNRALSENGAQVETNQSKLNRLQNEQAQLVSESEKLNSSFK
                     LQESALGSTASEADKLALAEQKVASHSEILEKQIHNLEQQLSLTKSEYGENSIEANKL
                     EKTLNETKTAYNNLQNEMEELASSSASSKASLEETNSLLKADLLMEFGDQLGELSQKL
                     IDFGQQSLDAFLEVDEGMDIIVTKTGATGSALEEMTDIAKTLATELPTDFNTAGSAVG
                     ELNTQFGLTGDALKSASTQLIQFSEINGSDVTSSALSAKQAIEAYGLEATDLSSVLDT
                     VTYTSQATGVGVQELMDKAVVGAPQIKALGLSFDEGVTLMGQFEKAGVDSSAALSSLS
                     KAAVKYAGDGLTLQDGLAGTIEQIKTSTSETEALSLASEIFGSKAAPRMVDAIKRGAL
                     SFEDLAGTADKAAGIVTQTYEGTLDPIDQFTTAQNTAKLAMAEIGDAIAATLAPILEI
                     LASLLQAVATWFSGLSEPVKQFIVIVGSLVAALGLVLPIFIALQAATMAMGTTIMGVV
                     TAAAPIVGIILGVIAAIALLVVGIQQLWQHHEGFRTAVTEIWNAIYAFLSVIIQQISS
                     FVMSIWGTLTTWWTENQQLILNAANTVWTAISTVIQTIMTILGPYLQASWENIKLIIT
                     TAWDIIKVVVETAINVVLGIIKAVMQIITGDWSGAWETIKQVVSTVWEAIKSLISIVL
                     NAIAQFISNSWNGIKGTMTNLLNSIKGVVSNVWNGIESTISSILSSIGSTVSSVWNGM
                     KATISGVLNGISNTVSSVWNGVKSTITIAINGAKNAVSSAINAIKNLFNFKIKWPHIP
                     LPHFSVSGSANPLDWLKGGLPKISIQWYAKGGILTKPTAFGMTGDSLMVGGEAGREAV
                     LPLNNQTLGSIGRSIAATMPNKGTSITVNITDVVIREEADMKKLADYVAGRLADEMNR
                     QALLRGGTV"
     gene            complement(32293..32712)
                     /locus_tag="ACUBV3_000099"
     CDS             complement(32293..32712)
                     /locus_tag="ACUBV3_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3846638.1"
                     /translation="MRQNITIAGKTYPLATNAYTPIAYKEQFGKDYFQDLFNMLSAES
                     IMTQLEQLEEGEELKASQIDLSILSDFDMTFFHRLFWVFAKSANPRIKPFEDFFMSME
                     EFPLQEVGPVLMSMLNQGMTTRKKQKLPKQRARKSSR"
     gene            complement(32724..33293)
                     /locus_tag="ACUBV3_000100"
     CDS             complement(32724..33293)
                     /locus_tag="ACUBV3_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HHU3846639.1"
                     /translation="MAEKNKVTFGLQDVHWAEVTSEGAAGALTYGTVERLRGAAELTL
                     EPTGDKGSYKADNINFYTSESNDGYEGTLKVALLSQEFLTRVLGEQLDVTTNTISEIA
                     SSKKKNFALMFRFEGDKKETLHVLYYCYASRPTVGSKTKSGSDINEVELTFTASPRPL
                     DKIVRRRTTEETSDEIRENWFKSVFEPAA"
     gene            complement(33296..33622)
                     /locus_tag="ACUBV3_000101"
     CDS             complement(33296..33622)
                     /locus_tag="ACUBV3_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3846640.1"
                     /translation="MRFEELFRVLKATKLPVAYHHFEEGHSPSPPFMVYLVTDSDNLG
                     ADNWTYHKRLNVQIELYTTKKDLATEKTVESVLDAHRLYFDKVETYITSEKLYQTIYS
                     ITLLGG"
     gene            complement(33631..33999)
                     /locus_tag="ACUBV3_000102"
     CDS             complement(33631..33999)
                     /locus_tag="ACUBV3_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HHU3846641.1"
                     /translation="MTKIGLDDLASVIEKELTTYAKETTDVMREVVEEVTDDAVDTLK
                     VTSPKRRGKYAKGWTSKTTTDTNTDLTKTIHNRTPGLTHLLEDGHAKQNGGRVEGRKH
                     IAPVEKKAIQSFEDKLRQKL"
     gene            complement(33992..34330)
                     /locus_tag="ACUBV3_000103"
     CDS             complement(33992..34330)
                     /locus_tag="ACUBV3_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail adaptor protein"
                     /protein_id="HHU3846642.1"
                     /translation="MKIAPLREQLVFQEKRLKQDDIGNESAIWDDLFMRWCSCRPLAL
                     TESDGSATKLIHNKVQFTLRYDKKVLVLNSLTTRIFFRGQFYAIESIDGDTVPRSLIY
                     IVTTKEELYD"
     gene            complement(34330..34587)
                     /locus_tag="ACUBV3_000104"
     CDS             complement(34330..34587)
                     /locus_tag="ACUBV3_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HHU3846643.1"
                     /translation="MMTLEEVKLYLKVENGEEDYLIEQLMTTSRQLCEDILRETSTSE
                     VLKTAILYGVAYLYEHREEANHKELKETLYHLLLADRKDVF"
     gene            complement(34584..35792)
                     /locus_tag="ACUBV3_000105"
     CDS             complement(34584..35792)
                     /locus_tag="ACUBV3_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HHU3846644.1"
                     /translation="MSKLLELKEKRNAAWAQAKAFLDTVRSEDGLVSDEDSKRYEEME
                     SKIELYNKEIARLERQEKIDLELAQPTSQALTTQPTVIVDNQKEDEKKGVASDIYTQT
                     FWTSVRKRNFYDVKDVLRVGEDTEGGHLVPDEYEKKLIQGLQEENFFRSLATVIKTSS
                     GERKIPVVTGHGSASWMDENGLYPETDETFGQVTLDSHKIGTAIRISEELLNDSVFDL
                     ESYMTSEFARRIGTEEEKSFLVGDGSKKPTGIFTQADVEGPTTATKDITFDDMIELYH
                     SLPAPYRKNAVWILHDTTVKAIRKLKDNNGNYIWQPSTQAGQPDLILNRPYYTSTFAP
                     LPEAGNKAIAFGDFSYYWIADRQGRTFKRLNELYANNGQIGFLASQRVDGKLVLPEAV
                     KTLTIKGKTA"
     gene            complement(35805..36503)
                     /locus_tag="ACUBV3_000106"
     CDS             complement(35805..36503)
                     /locus_tag="ACUBV3_000106"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003084543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HHU3846645.1"
                     /translation="MRKFWNFTDEGEVRTLRIEGQIADETWFGDEVTPQLFKNDLLAG
                     TGDITLWINSPGGDVFAAAQIYNMLMDYQGDVHVIIDGLAASAASVIAMAGTTVSMSP
                     VAMMMIHNPWTFAQGEAKDMAKVIEMLGEIKESIINAYELRTGLSRTKISHLMDSESW
                     FNAKKAVELGFADKVLFEKEETPEQDHQNSYTFSRVTAAHDLVVKLQASLQPPKPQKT
                     IPFNQLEKRLNLLK"
     gene            complement(36496..37791)
                     /locus_tag="ACUBV3_000107"
     CDS             complement(36496..37791)
                     /locus_tag="ACUBV3_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HHU3846646.1"
                     /translation="MGLLDVLGLKRARAEPVARNSYEGQDFSYLFGRTTSGENVDEFK
                     AMQTTAVYACVRILAEAIASLPLHVYGRTTNGKERKMDHPLYFLLHDEPNPEMSSFVF
                     RETIMSHLLIWGNAYVQIIRDKAGRVISLYPLLPDKMSVHRDDSGKLYYKYQRQTEEN
                     PNFKDKGTVLLKQEDILHVPGLGFDGLIGYSPIAMAKNAIGMTLATENYGAAFFKNGA
                     NPGGVLEHPGILKDPKRVRDSWNAVYNGATNAHKVAVLEEGMKYTQVGIPPEEAQFLQ
                     TRKFQINEIARLYRIPPHMVGDLEKSSFSNIEQQSLEFVKYTLDPWVVRLEQAFKRSL
                     FLPEEKKRYLIKFNVDGLLRGDYQSRMNGYAIARQNGWLSTNDIRELEDLNLLSDEEG
                     GNLYLINGNMTKLKDAGGFMKQPTETEPAEGPPEEEEDA"
     gene            complement(37860..38063)
                     /locus_tag="ACUBV3_000108"
     CDS             complement(37860..38063)
                     /locus_tag="ACUBV3_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000155124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3846647.1"
                     /translation="MTIKEIQEKIVAELRDKVSAGEFSENDAVAYWSDETGVYAYSLE
                     EKDIEHHSSVTSIRDLIETILES"
     gene            complement(38133..38471)
                     /locus_tag="ACUBV3_000109"
     CDS             complement(38133..38471)
                     /locus_tag="ACUBV3_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0090729 - toxin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HHU3846648.1"
                     /translation="MTNHKRYHVLLADHAKQDLRDIHDYILVNFYSQQSADGKIDLIL
                     SALETLETFPEACPLVSSRGYGQLTDDGKIYRYMPIENHIAFYFIEQHTVNVARIMNS
                     KQNWAKLFNK"
     gene            complement(38464..38757)
                     /locus_tag="ACUBV3_000110"
     CDS             complement(38464..38757)
                     /locus_tag="ACUBV3_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HHU3846649.1"
                     /translation="MANTQPVNFRADSAFYQQTKAILADEKLTLSDVFNAALRKIATG
                     AVDAKEFVSSDLTESQYQIAFEDLKKEILLGHQDIMEGKVTALSDVRKEFGLD"
     gene            complement(38824..40416)
                     /locus_tag="ACUBV3_000111"
     CDS             complement(38824..40416)
                     /locus_tag="ACUBV3_000111"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006268711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140657 - ATP-dependent activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HHU3846650.1"
                     /translation="MTYHYEPSPFMLPTSHYDKAKADRAVTFINNLAHTKGKWAGKKF
                     DLLPWQEQIVRDLFGIVKEDGNRQFLTAYIEIPKKNGKSELAAAIALYLLYADNEASA
                     EVYGAACDRNQASIVFDVAKQMVLMSRPLEKRSKIMGATKRIVNYSNAGFYQVLSAET
                     GTKHGLNVSGLVFDEIHAQPNRHLYDVLTKGSGDAREQPLFFIITTAGTDKNSICYEL
                     HTKALDILKGRKQDTSFYPVVYGLSDEDDWNDEANWLKANPSLGHTIGIDRVREAYQQ
                     ALDNPAEENVFKQLRLNMWTSSSVAWIPEHVYAKGNAPIDYEALKGRDCYAGLDLSST
                     SDITAFVLVFPPRHSEENYIILPFFWLPEDTLELRCRRDHVLYDVWERQSYIKTTEGN
                     VVHYGFIEAFIEQLSETYHIKEIAYDRWNATQMVQNLEGMGLTMVPFGQGYKDMSPPS
                     KELYKLMMEGKIQHGGHPVLKWMGQNVVMRQDPAGNIKPDKEKSVEKIDGIVSLIMGL
                     DRCIRHQGDETSVYDERGILSF"
     gene            complement(40413..40886)
                     /locus_tag="ACUBV3_000112"
     CDS             complement(40413..40886)
                     /locus_tag="ACUBV3_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HHU3846651.1"
                     /translation="MAVRGRKPKPTNLKILEGNSGKRPLPINEVKPKQKAPRCPQWLE
                     DDAKKEWKRMGKILEQMGILTEMDMTAFAGYCQAYARWKEAEEFLSKHGSIIKTPNGY
                     LQQVPQVSISQTNLKIMLKFCEQFGLTPSARNRLATMDAEVGTGDEMEDLLGGIL"
     gene            complement(40935..41384)
                     /locus_tag="ACUBV3_000113"
     CDS             complement(40935..41384)
                     /locus_tag="ACUBV3_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4314 domain-containing protein"
                     /protein_id="HHU3846652.1"
                     /translation="MNDKILNRIKSTYPEGTRVRLVQMDDPRPVPVGMLGTVLDVDDI
                     GSLLVSWDNGQSLNILYGIDSVVKVEPDRDGFIQSLYNYLRQGYNGYVFDSGEKAVPL
                     SHDEVISLMTSPTARVEIDVFEDERHYHNLEPSVKLSREEIESVVKN"
     gene            complement(41386..41637)
                     /locus_tag="ACUBV3_000114"
     CDS             complement(41386..41637)
                     /locus_tag="ACUBV3_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3846653.1"
                     /translation="MTTTTLEKLYSHYPATASILPYKDWVIVATPTYKGIVAEIYKYE
                     SLSEYTNRMEADLNLEKTSEETFQDQGHAVKWAFETLGA"
     gene            complement(41699..42979)
                     /locus_tag="ACUBV3_000115"
     CDS             complement(41699..42979)
                     /locus_tag="ACUBV3_000115"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HHU3846654.1"
                     /translation="MTLTFLDFFAGVGGFRRGLELAGMTCLGYCEKDKFARKSYEAMY
                     DTEGEWFHDDITSIDPTRLPKADLWTAGSPCQNVSIAGKRAGLYGERSGLFFTFVDLI
                     QSQKEEDKPEWVLLENVKGLLSSGGGRDYLDYLSILDEAGYDLEWQVFNSKDYGVPQN
                     RERIYTLGHLRSRGRRQVLPLSGEGGSYLKQLVGGMQSYRVYDPSGIATTLVGEGGGL
                     GAKTGLYLIDQSLTEPKLTEEARCITARYTAGATKRTAMNSGVLEVQPILTPDRITKR
                     QNGRRLKEQDEPMFTLTSQDRHGVLEGIKVRNGTKQDYQVAEVGDSVDLSYPGSQTRR
                     ARVGKGIAHNLSCGGQMGAVVWKGRVVKIRRLTPRECFRLQGFSDDLFDKAQAVNSDA
                     QLYKQAGNGVTVTVVYAIGKAILEANKSANNKQK"
     gene            complement(42976..44232)
                     /locus_tag="ACUBV3_000116"
     CDS             complement(42976..44232)
                     /locus_tag="ACUBV3_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HHU3846655.1"
                     /translation="MTTQPKMEIKELPLSDLKPASYNPRKKLKKGDKEYEKIKQSLLK
                     FGYVDPIIVNEDLTVIGGHQRLTVLKDLKYETAKCVIVSLSKEDEKALNIALNKITGQ
                     WDDQLLADLLLDLQESDFNLDLTGFEPPEIDDILSNVHDKDLTEDDFDVEEELKKPTV
                     ARRGDIWQLGKHRVICGDSTKAETYDQLLGDKKANLVVTDPPYNVNVEETAGKILNDN
                     MSDGDFYQFLYDMFTQVESHMEADASIYVFHADTEGLNFRKAFKDAGFYLSGCCIWKK
                     NSLVLGRSPYQWQHEPCLFGWKQKGKHQWFSDRKQTTIWEYDRPKSSKDHPTMKPIHL
                     MAYPIQNSSMRGTLVLDPFLGSGSTLMAADQTGRICYGIELDEKFVDVIVKRYMESTA
                     NSDVTVLRNGQTLSYDEAKAHMEDDL"
     gene            complement(44204..44659)
                     /locus_tag="ACUBV3_000117"
     CDS             complement(44204..44659)
                     /locus_tag="ACUBV3_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HHU3846656.1"
                     /translation="MNDNQRRGIWKLRRDGFGYGAIAQMLNLSLGSVKQYCRRHPELK
                     GVGQFVKYQLDEGERPYCKNCMKKLHHAVQGRPKKFCSNSCRAIWWRNHQSQHDKTKT
                     AYDELTCQNCGEFFLSYANPKRKFCGHPCYVEYRFRKGVSNDNATQDGD"
     gene            complement(44948..45313)
                     /locus_tag="ACUBV3_000118"
     CDS             complement(44948..45313)
                     /locus_tag="ACUBV3_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HHU3846657.1"
                     /translation="MPRRPSTPCKQHGCPNLVTYGNKYCDDHKANHTLDAKSTKAKGY
                     NSRWNKARLRYLKLHPLCVYCQRKGRLTKATVVDHVTPHRGDQDLFWNQSNWQALCKS
                     CHDRKTKTTDRYVEYTYRF"
     gene            complement(45315..46352)
                     /gene="metK"
                     /locus_tag="ACUBV3_000119"
     CDS             complement(45315..46352)
                     /gene="metK"
                     /locus_tag="ACUBV3_000119"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HHU3846658.1"
                     /translation="MIYTSEQVSSGHPDKLCDQISDAVVTECLKHDKNSRVAVETLIK
                     DNQVVVAGEVSTKHFFNLEGIVKKVLEPYGMEDIMVTNLLGVQSPDIAQGVNKGGAGD
                     QGIMFGYATDETPEFLPLPYVLATRVLEKLMNLGHPALGKDAKAQVTYDYENKRIETF
                     LVSIQHDEEVDLASVKRIVTQTMMSVAQRYRQNLDFRVLVNPTGCFVLGGSYADAGVT
                     GRKIVADTYGGFAHHGGGAFSGKDPSKVDRSAAYMARKIAKDFVREGYAKRCEVQLAY
                     AIGVAEPVGVYVNTFGTSDYLLEQLVGVVRERYDLTPQGIIKELNLLDVDYTKTTCLG
                     HFTKPYLPWEQ"
     gene            complement(46400..46582)
                     /locus_tag="ACUBV3_000120"
     CDS             complement(46400..46582)
                     /locus_tag="ACUBV3_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6900 domain-containing protein"
                     /protein_id="HHU3846659.1"
                     /translation="MTKCQQEKLNTLLTAIAQEELLVETLETRMSDNLDFYDVSVWGI
                     KRALERAYEAGQQSVK"
     gene            complement(46776..47252)
                     /locus_tag="ACUBV3_000121"
     CDS             complement(46776..47252)
                     /locus_tag="ACUBV3_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014622610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3846660.1"
                     /translation="MDKAEYILTHYNELKGDLEMLKYRLENFKPVTENEVIGSLVFER
                     SDEPKVTSTPTNLRSEVIALSFRDKMIQENEELLADLTQRYIRLANDLDNFEIAIRFL
                     KDDLAEFAQEMLKPECNWDSLMREFHISRSTVRNWRRKVLDHVRGVYLKMGYSLEK"
     gene            complement(47245..48621)
                     /locus_tag="ACUBV3_000122"
     CDS             complement(47245..48621)
                     /locus_tag="ACUBV3_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006268649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HHU3846661.1"
                     /translation="MKLSLHDYQEVTKDFIIRTPYAAVILDMGMGKTATTLSAINELM
                     FDRYEVSKVLVIAPLRVANTVWSDEIEQWEELRHLRYAKIVGTPKQRRAALEQDADIY
                     IVNREILPWLVQQCSPYFKWDMVVIDELSSFKSWQSKRFKAFMAMRPYMKRVVGLTGT
                     PSSNGLMDLFAEFKVIDSGERLGRFIGEYRSRYFDEGRRNGNIVYEYIPMDYAECQIY
                     DKIDDITISMKAMDYLDMPELISTKKVVHLTDKETDNYKRFKKDYVLSDLEDGEVTAA
                     NAASLSNKLVQMANGAVYSDDHQVVSLHDQKLDTLEDIIEAANGEPVLVAYWFKHDLQ
                     RIEERLAKLKVQGTVLKTEQDIREWNKGNIRVGLLHPASAGHGLNLQKGGHHLVWFGL
                     TWSLELYQQTNARLWRQGQQAETVVIQHIVTEGTIDEEILKALENKDAQQSRLIEAVK
                     AQVGGTDG"
     gene            complement(48602..48883)
                     /locus_tag="ACUBV3_000123"
     CDS             complement(48602..48883)
                     /locus_tag="ACUBV3_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001208489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HHU3846662.1"
                     /translation="MREKVVEQKLVSEVKKRGGICPKWVSPSFGGVPDRLVFLPKGKF
                     GMVEVKAPGGKPRLLQVTRHRLFERLGFKVHVLDSVEKIGEVLDEIEFT"
     gene            complement(49164..51452)
                     /locus_tag="ACUBV3_000124"
     CDS             complement(49164..51452)
                     /locus_tag="ACUBV3_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage/plasmid primase, P4 family"
                     /protein_id="HHU3846663.1"
                     /translation="MQFTLSHSGQTGIQTTTVYPHQVMISDKTTLQKVALFDHVAGLF
                     TNNTRSNANFIKSDVLVMDIDNDHTDNSDEWVTEELLKDIFADYNFALVTSRNHLVQK
                     GDKAARPKFHIYFQINEITDKDTYALLKEELVNRYGIFDTNAKDAARFFFGNPNAQVL
                     WHDSWLTIDEDLLDDMVAQDDEEDFDADFYQPTTGPITEGSRNSTMSVFAAKILKRLG
                     VTKEARDGFDEQALKCVPPLEKAELDTIWGSAVRFYNKTIKNSKDYKSPEEFQRESLK
                     PDDYSDVGEAGVLAREYADKLAYTNATDYLFYDGTHWRENKQLALGAVVHFTDDQLAE
                     ANTFLETTEKQLQSSGIDEMTIKAGGKRLENAVETSLQLKYLKAYLAAKEFHKFVMKH
                     RDYKNLMAVYNTAKPMLTVELSELDSDDMLLNTPEATYDLRQGTKGQQEHNPEDYITK
                     MTAVSPSDKGQDLWRETLATFFCNDQELIDYVQEIIGMAAIGKVYQEHMIIAYGGGAN
                     GKSTFWNTIARVLGSYSGKLSADALTMSNKRNVSPELAELKGKRLVIASEMAEGMRLN
                     TAVVKQITSTDEIQAEKKYKDPFHFVPSHTLVLYTNHLPKVGANDDGTWRRLVVIPFN
                     AKITGRSDIKNFADYLYDHAAPAIMSWIIEGAEKAIKANFKTSVPSAVSNSVKAYREA
                     NDWLGHFLSDCCEVGDQFTEKSGELYSQYRAYCTKNMEYTRSTTDFYSALDQTGFRRK
                     RTNKGNLILGLKLVDDDYDFLD"
     gene            51811..52134
                     /locus_tag="ACUBV3_000125"
     CDS             51811..52134
                     /locus_tag="ACUBV3_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000077671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3846664.1"
                     /translation="MSQHKQLLELITEMEGTAKYYLRLVDEFKKLLSTEEETDTISEE
                     PKPKPQKELKLEDVRAVLATKAKDGYKNEVRALLNKYGAESLSALATEHYAAVLEEAG
                     GIGND"
     gene            52127..53248
                     /locus_tag="ACUBV3_000126"
     CDS             52127..53248
                     /locus_tag="ACUBV3_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001891681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2800 domain-containing protein"
                     /protein_id="HHU3846665.1"
                     /translation="MTNHAVLSASASHRWLNCPPSVRLTEDMPDVTSEFALEGTDAHE
                     LCAYLVEKALGRKVRDPTKDLSFYNEEMQTCAEEYRNYVMEQVEKAKGYSRDPTVLVE
                     QRLDFSKWVPEGFGTGDCLIVADGLLQVIDYKHGLGVLVDADHNPQMMCYALGALEMF
                     DDLYDFDKVTMTIFQPRKNNISTFEIDKTELLEWAETELSPKAELAFKGEGEMTSGKH
                     CQFCKLKNVCRKRAEDNLALAKMEFADPATLDYEDIAEILPKLDLLVSWANDVKAYAL
                     KEATEGHNIPGYKLVEGRSVRKFSDEAAVSQAVMEAGFDPYEKKLLTITAMSKLLGKK
                     TFNDLLGGLIVKPSGKPTLVPLDDSRQEMNLAKNEFKEV"
     gene            53253..53816
                     /locus_tag="ACUBV3_000127"
     CDS             53253..53816
                     /locus_tag="ACUBV3_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2815 family protein"
                     /protein_id="HHU3846666.1"
                     /translation="MSTKVQTTKVITGKNTRFSYLNANEPKSINGSTPKYSVSLIIPK
                     DDIETVNKIKAAIELAYKEGEAKLKGNGKTVPALSILKTPLRDGDLERPDDDAYRNAY
                     FVNANSPHKPGVVDGNRQEIIDTSELYSGIYGRASISFYAFNSNGNKGIACGLNNLQK
                     LRDGEPLGGRTRAEDDFATDDDDDFLN"
     gene            53835..54002
                     /locus_tag="ACUBV3_000128"
     CDS             53835..54002
                     /locus_tag="ACUBV3_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3846667.1"
                     /translation="MDIYTILLCIILGIYVFLGLYLNYMTIRDDIRREKERKAEKKRQ
                     DNNTTPLHRSR"
     gene            54051..56006
                     /locus_tag="ACUBV3_000129"
     CDS             54051..56006
                     /locus_tag="ACUBV3_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017769760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HHU3846668.1"
                     /translation="MPIKELSIDLETYCEVDLRKSGVYRYAEDDSFEILLLAVSVDNE
                     PVTVYDLTKEELPDEILQALVDDTIIKWAFNASFERICLSNWLKKHHPELLSEGFLSP
                     NSWRCSMVWSAYLGLPLSLEGVGTVLKLKEQKLKEGGDLIRYFCLPCKPTKVNGGRTR
                     NFPSHSPDKWSAFIDYNQRDVEVELAIKEKLRNYPVPVFVWEEYHQDQIINDRGIGID
                     VDFVNAAIAIDGESKTKIQEELRKLTRLDNPNSVLQMIGWLREHGVTTDSLDKKSVKK
                     LLKTVDEKTAQVLKLRQQAAKSSVSKYQAMMNCVCKDGRARGMFQFYGANRTGRWAGR
                     LVQLQNLPQNHLPDLEEARKLFKTGDLEATELLYNTQYTLSQLIRTAFVPSDRKKFIV
                     CDFSAIEARVLSHLAGETWRSRVFEKGKDIYCMSASQMFGVPVEKHGQNADLRQKGKI
                     AELACGYGGAVGALKAMGAIDMGLEEQELQPLVDSWRQANPSIVLFWWDVDRAVKTAV
                     KEQIQTETHGIQFEVRNGMLFITLPSGRKLAYVKPKMGENQFGGESVTYEGTGTAKHW
                     ERLESYGPKFVENIVQAISRDILAYSMRQLSEFKIVGHVHDEVIIECDQDQDLEEIST
                     LMGIAPDWMSDINLRADGYECSFYQKD"
     gene            complement(56068..56610)
                     /locus_tag="ACUBV3_000130"
     CDS             complement(56068..56610)
                     /locus_tag="ACUBV3_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3846669.1"
                     /translation="MSKINKNSLYFHLITQDYFDTYQYLDEFQEDNARGHGVMVLDIN
                     NQLIAIPLRSGLPEHLRNASHLFPYTTYRRHDGRICLKALDFSKLTIIEEKYIDNSRI
                     YHFKNPNEKIFYLRNSNRIFSRVKNYVNKYIEICSKIEKGETVTFRTLNPYRFSTLRN
                     FHDELGIAISKEDFINQLRK"
     gene            complement(56772..57368)
                     /locus_tag="ACUBV3_000131"
     CDS             complement(56772..57368)
                     /locus_tag="ACUBV3_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3846670.1"
                     /translation="MTNKDNIYNECQKQECSLEVGLLEGQMLAPMLVENQQVIEDMRL
                     KRETLRSWRPAKQLNVYVLVAFIPVFPEEYAKTERIYKDAVENFLNEFRTRKSKNIEE
                     IISYEAWIDMDESTLYSSTEELEEFMFAEMLQDLLEKVRQADKDLYDITLGLLAKEFD
                     ESIEDVFARIGVKRSTGFYRLPKARALVAALLKKFNPK"
     gene            57801..58925
                     /locus_tag="ACUBV3_000132"
     CDS             57801..58925
                     /locus_tag="ACUBV3_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000347290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-three component system protein"
                     /protein_id="HHU3846671.1"
                     /translation="MDFPTYFRILKKYLGDGATIPEFFRELIEMITDDDAEDIISASG
                     ITSDKADSTLISYTKPKRGFTKKMANQLLYRVNSANMTESIESRPDETIQLLANEFNT
                     YYPDITAENASQRIPEIFVDFIREKAGMGISTTAQKATFINQSNQLKKQYGQFLLTEA
                     NNCCAFPGCDRPLILTRGGLASENYEVSTIEKDKDAEPLNLIALCPDCFLTYQAESRK
                     KIVTALKNVKKILVSAHNSQQSISDMKLDSGIVSVLTSLNKLKFDEYDISYDPKQLTD
                     KISPENNRTLYQLVKNQVIDNYLTIQKIIINLDKQGRIDYEDIQYQMRSMYKKLKAAK
                     HGNLEIFNTISEKLHKATLQDIYFCQMIVSYFIQKCEVLE"
     gene            58925..59149
                     /locus_tag="ACUBV3_000133"
     CDS             58925..59149
                     /locus_tag="ACUBV3_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-three component system middle component 7"
                     /protein_id="HHU3846672.1"
                     /translation="MQLPNKLYSYQKSTLAYLPRVLHEIKMGNSNVKDIFHTISEELE
                     DPTDFLSIMDCLYTLNAIEMTNEGEVTLCL"
     gene            59140..60798
                     /locus_tag="ACUBV3_000134"
     CDS             59140..60798
                     /locus_tag="ACUBV3_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2326 domain-containing protein"
                     /protein_id="HHU3846673.1"
                     /translation="MLIEMWSPVFKKDGQTREPIQFHPGLNVIMGMDLANNSIGKSSS
                     LLAIDFIFGGNSYLKSIAVKKLGDHPIYFCFQFEKKFYFSRDTANPDIIIVCDKNYSP
                     TGEIMDLGTFLNKLKKRYHLESSDLSFRLAMSGFFRIAGKNNQNTDFPLQVYASQKSS
                     ESITTLIQLFNLYDNIARYKERLKDKSDQLTTFRNARKYEFISNLVGGKKQFELNVSE
                     IKRLEYGLSHLQDTYQDNICSDDIEKHQQKLQLRNTKLELESILRDKQRRLKLLDISI
                     EFGLYPTESDLTELQQYFPDTNLKKLYEVETYHKKLATILDSEFSTERESLVGEIDEL
                     ESQLVTLNQELQELGSIPNLSTEFLENYSKLTATINALKEQNEAYLKESALSKEKSEA
                     DSDLKRSTEDILIELEGKINAKMREFNNILYPDIRKAPQINLKAHNSYSFYTPDDDGT
                     GTKFKGMILFDLTMLYLTNLPALAHDSLLLSNISYQATEALLKLYDQSKSLNKQVFLA
                     FDKASSYSPEANQLLLENTVLRLSSNGNELYGISWNKGENSDEV"
     gene            60788..60997
                     /locus_tag="ACUBV3_000135"
     CDS             60788..60997
                     /locus_tag="ACUBV3_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HHU3846674.1"
                     /translation="MKFSYNKLWKLLIDKDWTKTKLRQEAGISSSTIAKLGKGENITT
                     DILLKICITLDCKIEDIVEIIDNDD"
     gene            61256..61606
                     /gene="tnpB"
                     /locus_tag="ACUBV3_000136"
     CDS             61256..61606
                     /gene="tnpB"
                     /locus_tag="ACUBV3_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HHU3846675.1"
                     /translation="MTINLSDLGQVYLVCGKTDMRQGIDSLAYLVKSQFDLDPFSGQV
                     FLFCGGRKDRFKALYWDGQGFWLLYKRFEKGKLTWPNNEYDVKALTSEQLNWLMKGFF
                     ITPNINCTKSRDFY"
     gene            61660..61845
                     /locus_tag="ACUBV3_000137"
     CDS             61660..61845
                     /locus_tag="ACUBV3_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HHU3846676.1"
                     /translation="MEELLKIIKQQSNEIALLREQVVYLTQKIYGKSSEKTIQINGQL
                     SLFEEEQLTEEKADLPR"
     gene            61817..63088
                     /gene="tnpC"
                     /locus_tag="ACUBV3_000138"
     CDS             61817..63088
                     /gene="tnpC"
                     /locus_tag="ACUBV3_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HHU3846677.1"
                     /translation="MKKKRTYPGELETITYQRKKAKGKRQTILNQFKAEERHYHLTEC
                     ACPDCHGALKEIGATVQRQELVFIPAQLKRVDHIQHAYKCLACSEKTLSDKIIKAPVP
                     KAPLAHSLGSASIIAHTIHQKFHLKVPNYRQEEDWTKLGLPITRKEIANWHIKASQYY
                     FEPLYELLHQKLLEQPVLHADETSYRVLESDSQLTYYWTFLSGKDEKEVITLYHHDKR
                     RSGLVAQEFLGDYAGYVHCDMHGAYRQLETAKADKSSLVFKGLCYCDKMFALEESWAD
                     LSLQDRLMKRQDELEPLMAEFFDWCRRQAVLPGSKLGRAIDYSLKYEETFRTVLKDGS
                     LVLSNNMAERAIKSLVMRRKNWLFSQSFEGAKSSAIIMSLLATAKRHHLDSEKYIGYL
                     LENLPNEEIFAKKEVLEAYLPWAEKVQQNCR"
     gene            complement(63369..>63472)
                     /locus_tag="ACUBV3_000139"
     CDS             complement(63369..>63472)
                     /locus_tag="ACUBV3_000139"
                     /inference="COORDINATES: protein motif:HMM:NF012948.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HHU3846678.1"
                     /translation="LPSTGEINTHLSLFGIIVLLISTLMYGSKKKED"
BASE COUNT        18720 a        13884 c        11861 g        19007 t
ORIGIN      
        1 ggattttaca tttattatat tgcttttctt tttcgaagat aagttaatcc tacaatagct
       61 aagataacaa caacgataac gaatagaact gcaaattgac taccaattat agctccattt
      121 ttcggaatag ctacttgtcc gatggtaaaa atttgtgagg ctactgcggt tgcaaaagct
      181 cccgcaaatt gttgtagtgt atttaagata ctattaccat ccgctgactg tgctggcttt
      241 agttttgtta aagctgtcgt aaccatatta cttgctccaa tccccatacc aattttagta
      301 agaatatcaa aggctaaaag tactacaaca aacttcgcag gaatgaatat agctgtacca
      361 attaaaccta tcaaagaaag ggagatacct atcataattg gcttaaatgc accaatttta
      421 tccaggagat aacctgcaaa aggggataaa aaaactactg ctattgcacc tggaaacata
      481 aacaaaccag ctttggttga agtttgatgt aaaacaagtt gcaagaggtt aggtaataag
      541 aacgaaagtg ctagaggaat catttgaaaa gccaggacgc catacaataa gattgagaaa
      601 tctttatctt taaaaacatt caaattcaaa aattctagct gtttatttaa atagtaaaaa
      661 ataacaaaac taacaatagc tactaataag taaaaaatag acatcttttc aatagctaaa
      721 agaagggttg ccaagccaat acctaaactg acaaaggcag gaaaattgag tttatcatta
      781 attcttactt gacgtttggg aatagaagcc aaacctataa aggtagatat gataagtatt
      841 ggtgccagaa gcataaaaat catcttccat cctaacattc ctgaaatgac accaccataa
      901 gtaggtccaa ctgccggtgc taaaagtgtg accatagcag ctactcccat cattagtcct
      961 ctacgctcca tcggaatttg tgtaagaatg atatgaaaca ataaagggag ggctaatcct
     1021 gtcccaacac cttgcaagac tctccctact aacaatatag ctaaattagg ggcaaagcta
     1081 tcaattaaaa caccaaataa aaaaagcaca tttgccaatg taaaaatttg tctctctgat
     1141 aaatttttaa tcataaatgc tgacaaaggc acagtcacag caacagctaa caaatttcct
     1201 gttgtcaccc attgaataac agcggggtta accccaaatt ctttcatcaa aagcggaaaa
     1261 gtgacattca tagatgtttc aataagaatc cctgaaaaac ctaatatagc cgttgtaatt
     1321 atggcaggca taatacgcca tcccttttta gtcattaaaa tctccttact ttgattatta
     1381 tcataaaata gagcaccccg ctaaatcaag gtgctccatt taattcattc gaattaaaat
     1441 taaattgttt atattagttg tcaaaggaca atcaacagct cttattatat caaataaagc
     1501 taagaaaaac aaagagtttt ttgaatatta atgccttcac tttcttatct attctctacc
     1561 catcaatcac cacttcagag aacataacaa ttgcaattta ctcttaatac cgttatcatg
     1621 ttcatatgct aatacccaag aaaatagtgg cttttgcctc aaatgaatct gaaaataatt
     1681 tctttattac gttaaagact tactttaatt atttgttttc tattcaaatc attacagata
     1741 tttcaactct caatcatgca gattttgacg gtagctttgc attccatgat atagaaacta
     1801 gcatacctca tctggtaatt gactccaact acttagcgat tagtcagact aactcaaaaa
     1861 cagaggctaa cgatattaaa acgttctcgg aactctccaa aacaatgacc gagttttact
     1921 atatgctaaa ttttgactta tttaaccatc tcccatatcg ttttagattg cataataagg
     1981 acggtcaaac aatttattcg aaccataaac ctgaagatcc ttttgatatt tatcctgaag
     2041 aagaatatcc tatagacaaa tgggttcaaa acagtctaat agagaaaaaa gcgaaagagc
     2101 tacatttact cttacctggc gcatcacaag attatattct agtacaatct tataaacgct
     2161 tagaaaatga tagtgggcaa ctagttggtt atattgaaca tgtccataat attaaaccac
     2221 tattagaggg gtatttaaaa gaatccggtc aagctatcgt tggatggtct gatgtcacgt
     2281 ctggtgcttc tatctctaac gatgattttg aactataatc cactcgtcac tctaaaaaga
     2341 aaactaataa ctaaaaggca ctggtttaat tccagcgcct tttattaatc aatctccagt
     2401 tgttgtaagt tcaatttttt ctttaattgg tagacctcga ttagctgtaa aaatcaaagg
     2461 aactacttct gttgtaactt cggaaaattg tagaccaaac catcgtattg gtgttgctgt
     2521 ccaatgcttt acagatgatt tcattagcat aacaaatcgt tcaccaggtt ttaggttttg
     2581 actgcttgaa aaacgttcat caaggacaat aaacctaaaa tctcctactt tactatcagg
     2641 tctaacgctg tatgtctttc cttgtttagg cagacgtcca ctttcaagta attcttctac
     2701 aatcgtacga agatagcgtg acacagtctg acgcatctta aatccaaggt aaagttctac
     2761 cttcacaatg aagtcggttc ccatcatgtc aactttatac tcagctgtat aaggttcatc
     2821 agtgacctta atgtttacaa accaataaac ttgtgctttt ttagggcgct tatcaaggat
     2881 agaatacatg atagacttat caatcatgtc tccttccata cgatttgtca aataaacaac
     2941 gttagtatga taaatcggat aacgatgatc atgtctaagt ttatctaatt gtccgatgta
     3001 atcttttaaa tccaacaact taacataacg cgaaacaatt ttactacctt tataccagat
     3061 agtcatgatg aatataattg ctactgctat aataacaaca acatagcctc catgcataaa
     3121 tttaacagct gaagcaagga agaagattcc ctctaaaata ccaaagaaaa tcatcatcaa
     3181 taagactaaa cctcttttta ctcctttttg aattaaaaag aaagacagta aaatagttgt
     3241 cattagcatc gtaattgtta tcgctaatcc atatgctgct tccatgtgcg ctgaagtctt
     3301 aaaaagcaaa acaatagagg ttgtaatggc aaataagaac caattaataa caggtatgta
     3361 ggtttgacca atattgtcac caggataagt tgaacgaaat tgtgggaata tttttagtcg
     3421 catagcttca gaaactaagg taaatgatcc agaaatcagt gcctgtgaag cgatgatagc
     3481 tgccaaagta gctaaaataa cgacatgcat tgtaaattgc gaaggaatac tagcaaaaaa
     3541 gggattcaat tcatttcctg cgttcttatt agctaaaatc catgcccctt gcccacaata
     3601 agaaagtata atggcaacct taacgaacgg ccatgaaaca tgtatatttc cacgcccaac
     3661 atgacctaag tcagagtata gtgcttctgc tcccgttgtc gccaggaaaa tagatcctaa
     3721 aataaaaata cctttatgat tctctggact aaataacaat ttcaaaccgt agtatggatt
     3781 aattgctttg aaaacttctg gatgggcaaa actattaagg agaccgctaa tacctaaaaa
     3841 ggcaaaccat ataaacataa ttggcccaaa taatttccca ataacacccg ttccaaacct
     3901 ttggatggca aaaagtaaca gtaaaataaa caaggtcgca aaaataacat tactctgatt
     3961 ctggaaaata tgctgcaaac taggaactac ttttaatcct tcaacggctg aagttacggt
     4021 tacagctggc gtcaaagctc catctgacaa caaggttgca cctccaataa cagccggaac
     4081 aattaaccaa ggtgtcattt ttctaacaag ggtatataaa gaaaaaatac caccttcgtg
     4141 gtgattatcc gcctttaaag ctacaagcac atacttgata gttgtaataa gtgtcaaggt
     4201 ccatatgatt aaagatatag aacctaagat aaacgattct gtgacactag aaataccacc
     4261 ttggttttca accaatgatt gcatcgtata gagtgggctt gtaccaatat ctccataaac
     4321 aatgcctaaa gcaataataa atcctgcttt tgatgcttta tcaaaagaag aatgattgac
     4381 atgttgcatt tctgccaacc ctccgattaa ataatattag tcaaaaaaat ggctgaactc
     4441 attatagcag attaagttaa aaaaacaaca cttatctata aaaagaccag aaattattaa
     4501 agcgtttcct ggtctttact attttaatca cgataaacag gttgaggtcc ataagtttga
     4561 ctgactggca ttatttctat gcgattaata ttaatgtgtt ctggttggct agcaacccat
     4621 gacacggtat tagcaatatc ctctgcttga attgcatgcg caccttcata aagtttttca
     4681 actcttttat gatctccttt gaaacgtaca gttgaaaatt ctgtcccttc acataaaccg
     4741 ggttctaggt tagtaactct gattttagtt ccagctaaat cggcacgcaa gttgagagca
     4801 aattgtttta caaaagcttt tgatgcgcca tagacattag ctcctggata aggaattgtt
     4861 cctgcagttg atcccaaatt gataattaac cctctattaa cttctaccat tttcggtaaa
     4921 atgcatcgtg tcaagtaaat taatcctaca acgttagtat taatcatagt catccaatct
     4981 tcaaaatcag cttcatatga tttatccaat ccaagtgcta aacctgcatt attaaccaac
     5041 aaatcaattg attgcaaatt tttaggcaga ctttctaaag ctttgtcaac cgattctcta
     5101 cttgtaatat ccatttaaag tggataaaag taaccctctg aaaacttttc tccaaggcat
     5161 tttaatttat ccattcgacg agcacaacca ataacgctat aaccatctga aactaattta
     5221 gtaacaacag ctgcaccaaa accagcagat gcccccgtca caagcgcaac tttacgtgat
     5281 atcataaata ctcctcctct agtatataga aaaacgagct aggaaaaatc cttctcgttt
     5341 tataacttat tttgatttct catcaatagc ttgagcagct gtaatgatag caagtttgta
     5401 aatatcttca gcacttgatc cacgtgataa atcatttact ggagcattca atccttgtaa
     5461 gatcggtccg attgcttcaa acatacccaa acgttgtgca attttatagc caatatttcc
     5521 tgattgaagg tctgggaaaa taaaaacatt tgcttttcca gcaacatcac tattaggagc
     5581 tttaatttct gcagtttccg gaacaaaagc tgcatcaaat tgtaattctc catcaacagc
     5641 taattcggga gataaatctt tggcaatttt agctgcttct tgaacttttt cagcttgagg
     5701 cgcttttgca cttccttttg ttgaaaaact aagcatagca atcttgggat taatatcaaa
     5761 tattttggct gtatccgcag tatttactgc aatttctgct agctcttgtg cattaggatc
     5821 aatgttaata gcacagtcag caaaaatata acgttcttgt gtattttcac ggttcatcaa
     5881 gaatacacct gaagtgcgtg aaattcctgg ttttgtttta ataatttgaa gagcaggacg
     5941 tacagtatca gctgttgaat gaatggcccc tgataccata ccatctgcca aaccaagttt
     6001 tactaacata accccaaaat agttaacatc cttcaaaaga cgatctgcat cttccatagt
     6061 agctttgcct ttacgaatct caacaaaagc ttctttcatc tcttcaaatt tttcgtattg
     6121 ttcagggtca ataacgctat aatcttgatc aacaaaacca agtcttgcca aagaatcgtg
     6181 aacttcgtca gccttaccta aaataacagg atccacaaga ccttcaaatt tcaaacgagc
     6241 tgctgcacgt aatacacgtt catcgcttcc ctcagggaaa acaatcttaa cataatcact
     6301 tgcaactttt tctcttaaac caccaaataa ggaacgcata tatataaaac cttctatttc
     6361 taaattaatg taattccatg ataacgctgt caaagtcatc tgtcaagtct agcgtctgtt
     6421 tatttatttt tcccataaaa gggtcgtaaa aagacaaaga atgacaatgc aatgcttgcc
     6481 tgtttattcc tagatccata cgtcctccgt acaaatcatc tcccaaaaga ggaaagccga
     6541 tatgagcaaa gtgaactcta atttgatgag tacgccctgt atgtaatttg atatccacta
     6601 atctaacatc accataacga gcaactactt gataactagt atgtgcatat ttgcctgatt
     6661 catggactcg cctcgtaata atactatcaa catctcgtgc aataggagct ataatatcac
     6721 cactatctgc taaatcacca ctccctgata ctaatgcata ataccttttt tcaattgctt
     6781 tagcctgaag ttgtttatct agacgagcat gggcataacc atgcttggcg aataacatta
     6841 atccacttgt atctcgatcc aaccgggtca caatatggac ctgctgattg gcatagttat
     6901 tagacacata atagtgtttt ataaaattag caattgtatt tgaatgaata gagctaggaa
     6961 tactagcgaa accaaaaggt ttatttaaaa tcaaaaaatg atcatcctca taaacaatat
     7021 ctaaatcgtg aggtactggt tccaaagttt cgtgagaagg ctcgtcagga atatctatcg
     7081 taacaacatc gccaatctct aataagaaaa tagcgttctg ctcttcacca ttgacaaaaa
     7141 tcttaccacc tctgtactta atctttgcta ataaccccct tgaaacatca tgacttttca
     7201 aaagtgtttt aactttgcac ctttcttctg ctatgtattc aaatttcaat gttcaggttc
     7261 tccgataaaa gcatcgttaa cacgttccca aaagctagtg tgacttggag ttgaaacaaa
     7321 gttaatactt ttttcgtcaa tactatattc tattttggta acatttttgt aatggaccgt
     7381 tttgttatct atagaaattg tatagacacc aactcgttta ggaactatct ctatagcatc
     7441 ttttttgggg atgataaccg atgaccccaa ggtacgatag acgcgattat taagactaga
     7501 aatttctgtt agttgcaagg cttctatcgt tgggtggagt actgcccccc ctagtgattt
     7561 attgtaagca gtactaccgg ttggtgtaga aactaaaatg ccatctcccc taaacctttc
     7621 aaataccact tgattaataa caacatccgc taccattgtt ttttcaatac gcttaatagt
     7681 agactcattt aaagcacgcg cacgaataac tcgaccatct tcaagggtaa tagttacttt
     7741 caaaatagga taggagattt gttcaccttt atcatttttc aaattattaa ttaatgtgtc
     7801 aacttcaaaa tctctatagt ctgtataaaa ccctaaatgt cctgtatgaa caccgacaaa
     7861 ccttacttta tccagttgtt tttcgtacat atggaaggct gacaaaagca taccatctcc
     7921 accaatagaa atgacaatat caggatcctt ttttgataaa taaaaatcag gatcatgttt
     7981 aaaagcagca aagagctttg aggctacacg cttgctttga tattttccgt tagcaattat
     8041 agcaacacgt gttgctctat ctgtaaaatt catctgtgtc atcactattt cctacaccat
     8101 cgctcaattt tcgatttaaa ggatcaaata aaagctgtgc ttcacgaata tcttcgcgaa
     8161 tcttacgcat ttcttcatcc aattctaaag cgatttttgc tgttttctcc agtctttgtt
     8221 taatttcttc agggaagtcc cccttatact tgtaatttaa tgaatgttcg atggttgccc
     8281 aaaaattcat agccagagtt ctaatttgta tttctgctaa aactttcttt tgtccatcta
     8341 ttgtgtctac aggatactct acaacgacat gatatgaacg atatccacta gatttcatat
     8401 gggtaatata gtctcgctcc tgaaccactg tcatgtcatg acgttttctc aacaaggcaa
     8461 ggacttcatc aacgtcatct acaaattgta ccatgatacg aagacctgca atatcttgca
     8521 agtcctgagc aagattttct tctgaaatac cacgtaaaac catcttttct tgaatacttt
     8581 caaccgactt tacgcgtcca gtaacaaatt ctattggaga gtgacgattt tgctttctaa
     8641 attgtttacg aataccacga agctttattt ttaattcacc aaccgtttga atataagggt
     8701 ctagaaatgt ttcccaatcc attgacattc taatcctcca aaatatttaa tgtactctct
     8761 tgtaaaataa actttttttc tataaaatta acacaacaat taatattata ccatatttag
     8821 gaaaaggaag tgataagatg acacacctag aaattgaata caaaactcta ttgaataaag
     8881 acgaatttaa tcgtctaaca agcttatttt ctcatgttca acctattact caaaccaatt
     8941 actattttga cacagaaaca tttgaaatga aagcacaccg aatgtcactt cgcattagaa
     9001 cgttacctaa ccgtgccgaa ctaacactaa aaatccctag agaagtagga aatttagagc
     9061 acaatcatga ccttacttta gaagaagcta aatatatcgt taaaaatggc cagttccctg
     9121 aagatactga gattgcttct ttaatccttg aaaagggagt tgatccaacc aaattagctg
     9181 tctttggaca acttacaaca acaagacgag agatggaaac ttcaatcggc ttaatggctc
     9241 tagacagcaa tatatatgct gatattaaag attatgaact tgagttagaa gtcaaacaac
     9301 ctaaacaagg gaaaagagat ttcgaccagt ttttaaaaga aaataatatt aattttaaat
     9361 acgccaaaag taaggtggct agattctctg ctactttaaa aaataactaa cgttgagaga
     9421 ttggattttc caatctcttt tataattctc atttaagcaa gaacttaaaa tctcatacta
     9481 tctctcctca acattgttat tgcaagcatt ttcatgtttc ttatcgtgaa ttttttctat
     9541 ttttttggta aactattagt atcaatctaa aggagtcatc tatctcatgg cagaacaata
     9601 cgccgataaa caaatcaaac ttttctcatt aaccgctaat agagaaattg ctgaaaaaat
     9661 ttcccaagct tcaggaattc ctttgggtaa gatgtcatca cgtcaattct ctgatggcga
     9721 aattatgatt aatattgagg aaacggtgcg cggtgacgat atctatatca tccaatctac
     9781 aagcttccca gtcaatgata atctctggga attactcatt atgattgacg cttgcaaacg
     9841 tgcaagtgct aatactgtta acatcgtagt cccttatttt ggatactctc gtcaggaccg
     9901 catcgctgca tctcgtgaac ctattactgc aaaattggtc gctaatatgt tagttaaagc
     9961 tggagttgat cgagttctta ccctcgactt acatgcagtg caagtacaag ggttcttcga
    10021 tatcccagtt gacaatcttt ttactgtccc actatttgct gaacactata atcaactcgg
    10081 tctctctggt gaagatgtcg tcgttgttag tcctaaaaat tctggaatca agcgtgctcg
    10141 tagtttggca gaataccttg actctccaat tgctatcatt gattatgcac aagatgattc
    10201 tgaacgtgaa gaaggctata ttattggtga ggtagaaggt aagaaagcta ttattattga
    10261 tgatatttta aatacaggaa aaacctttgc cgaagctgca aaaattttag aacgtggtgg
    10321 agcaactgag atttatgctg ttgctagtca tgggcttttt gctggcggcg cagctgatat
    10381 tttagagtct gcaccaatta gagaaattat cgtaacagac tctgttttat ctaaagagcg
    10441 tattcctagc aatattaaat atctgaccgc tagccatctc attgcagacg ctattatccg
    10501 tatccatgaa agaaaacctt taagtccatt attttcatat cgttcagata agaaggatta
    10561 attgatgatt tatttagaca atgctgctac taccgctcta accccatctg ttattgagaa
    10621 aatgaccaat gtcatgacaa gtaactatgg taatccatct agtatacata cctttgggcg
    10681 tcaagcaaat caacttttac gtgaatgtcg acaaattatt gctgaatatc taaatgttaa
    10741 ttcacgtgaa attattttca cttctggggg aactgagagc aacaatacag ctatcaaagg
    10801 gtatgctctt gcaaatcagc taaaaggtaa acatattatt acctctgaaa ttgaacatca
    10861 ttcagtccta catactatga cttacttatc agagcgattt ggttttgata ttacttactt
    10921 aaaaccaaac catggacaaa ttactgcaaa agacgttcaa gaagctttac gagatgatac
    10981 tattatggta tctctcatgt ttgctaataa tgaaaccggg gactttttac caattcaaga
    11041 gattggtcag cttctcagga accaccaagc tgtttttcac gttgatgccg ttcaagtctt
    11101 tagcaaaatg gaacttgatc ctcattcttt aggaattgac tttttagctg cttctgccca
    11161 taaatttcac ggtccaaaag gtgttgggat actttactgt gctccccatc actttgatag
    11221 tctacttcat ggtggagacc aagaggaaaa aaggcgtgct tcaactgaaa atataattgg
    11281 tattgctgga atgtctcaag ctcttaccga tgctacgact aacaccctta aaaattggac
    11341 tcacattagt cagctgagaa cgaccttttt agatgctatt tcagaccttg acttctatct
    11401 taataacggt caagactgct tacctcatgt acttaatata ggttttccta gacagaataa
    11461 tggcttgtta ttgacacagt tagatttagc tggattcgca gtttcaacag gttctgcatg
    11521 tactgcagga acagtcgaac ctagtcatgt cttaacaagc ttgtatggag ccaactcacc
    11581 acgtctaaat gaatcaatac gtattagttt ttcagaacta aatacccaag aagaaattct
    11641 tgaattagct aaaaccttaa gaaaaattat aggagattaa tatggcattt gaaaaagaaa
    11701 taaatcttcc agactgcaat tacatttaca ctatcagccc gaacattaaa aaatatacct
    11761 tacgcgatac tacattcact caaaatcgct taggtaatta tgaattagag cgtctcctag
    11821 aaacagtacc taattctggc gatggctttc ctctaaagat tacaatcaat aaagaacttt
    11881 ctggttttaa gctcttaatt actgataaat ctggccttag aatagtaaat atctttaaat
    11941 ctaccgatca taaaattctt caagacaaat tttatttctt aatggacagc ctcgttgagc
    12001 gtaatatctt tacgaaaact gcaaaataat ttaccgacca ctatttttaa cattttagtg
    12061 gtctttttgc gaaccatttt gttgtcactt tcacaatctt tttgtacaat agagatgtga
    12121 agaattaagt tatataactg attctaaatc gaataataaa atttttaaca aggagataat
    12181 tttgtgatta tggataagtc tattcctaaa gcaactgcta aacgtttatc actgtactac
    12241 cgtattttta aacgttttaa tactgatggc atcgaaaaag ctagttccaa acaaattgca
    12301 gatgccctag gtatcgattc tgctactgtt cgacgtgatt tttcttattt tggtgaacta
    12361 ggacgccgtg gttttggtta tgatgtcaaa aaacttatga acttctttgc agaaatattg
    12421 aacgatcatt ctacaacaaa tgttatgctg gtggggtgtg gaaatatcgg tagagctctc
    12481 ttgcattatc gtttccacga tcgcaataaa atgcaaattt caatggcttt tgatttagat
    12541 agcaatgatt tagttggtaa aacaaccgag gatggaattc ctgtctacgg tatttcgact
    12601 atcaatgacc atttaataga tagtgatatt gaaactgcta tcctaacagt acctagtaca
    12661 gaagcccaag aagttgctga catcttagtc aaagcaggta taaaaggcat cttgagtttt
    12721 tctccagttc atttaacatt accaaaagat atcattgttc agtatgtaga tttaacaagc
    12781 gaattacaaa ctttacttta tttcatgaac cagcagcgat aaatagttca ttatttataa
    12841 aatatctgct tcttctcgaa aactataata tttatttttt ccaacgatga tgtgatctag
    12901 gcagacaatt cccagatgat cacatgatcg ttttattttt ttagtgaaac ttaaatcact
    12961 ttcactggga tttggagaac ctgagggatg attatgtata ataatcaaag aagttgccat
    13021 gtttttacaa gcataatgta gaatttctct tggctctgct actgaacgac gtacagtacc
    13081 aataaaaata gttctctgtt cgataatacg attttgtgta tccatataaa tagctactaa
    13141 atgttcttgt tttttatccc ctaattcgag catcattttc ctcgctaatt gttcactact
    13201 taaaatttgc tctttacgat catattcagc tttgtgaatc cgttttgcta gttctagcat
    13261 agcttttatt tcgacggatt taacctgacc gattccttta atggattgca actcctgtaa
    13321 ggataattga ccaaaatctg ctaaactgct tatgttttct aaaatttgcg ttgaaatttc
    13381 aagaacaggt ttttctttaa tacctgtacg taagagaatg gctaataact cctgattact
    13441 caatctatct gcgcctaaat caactaggcg ttctcttggt agtaaagctt ccttttttaa
    13501 ttcaatatgg tacataaaac acctccagtc ataatattcg taaactaatc aaaaactcat
    13561 gttttaaatc aataaaaaat acttagatga aaaatctaag tattttttat ttaacgatag
    13621 gttcgtgcac gatttctaag cgtttgtcgc ttgataatat gatcttttac aatctgatag
    13681 atagtagctg caacaggaac agcaagtaac atgcctaaga tcccccataa agcacctccg
    13741 atagtaatag ccattaaaac ccacatagaa ggcagtccaa tcgatccacc gacaactttc
    13801 ggataaatga catttccctc aaattgttgt aaaaggatca agaaaagaac aaacaagaat
    13861 gcttcagtaa gcgattcagt agcaattaag atgaaaccga ttgtaacacc aatgtaggct
    13921 cccacaaccg gtattagagc tgtaaaagca actaaaaccc caactgttaa agcataagga
    13981 aattggaaga tcaacatacc aataaccgtc aaacttccta aaatcatagc ttctaaagtt
    14041 tggcttacaa aaaaaccatg gaaacgttga tgaaggatat gacgaacgta atggaatgtt
    14101 ttgcctgttg aacctaaata ggtatcaatt aacaaattaa attgacgtcc caactgctcc
    14161 ttgtttgcca aaacgtaaat tgaaaaaata aaactaacaa aaacattcag aagtgttgcc
    14221 gcaatagagg aaactgaggt tagtaaattt gttaaaacag ataaaacttg cttcaaaatc
    14281 tgctggctat aaccacttaa agtagaaact aagtctttat ctgttcccat ataatttaaa
    14341 gcctcagaaa tttgtttatt ttcattgaga ttattaacca atttagcaag tgctccggta
    14401 tcaataacca ataaagaact caaactagaa atcaaatctg gaatgacaat tgaaaatagc
    14461 cagacaatta aaccaataaa agttgcatag gataaaatca tagaaacact acgcttgatt
    14521 gccattaata gtctagatcc tttaaaaagc tttatatata atcgctcata gactgacatt
    14581 acaatattaa taatataggc aattccagct cccatcaaaa aaggaaatat tgccttatga
    14641 agcgtagtta aaatattaga aacagctccc caataagcct gtatagcata gcaaatagca
    14701 aatgttatga caacataata gacctgccgt ttttcaaatt tcatgaaatc tccttattaa
    14761 tcttaatttc tttcaattat tatacactat tttatgaatg agaaaaagag agttaacagt
    14821 tattagtctg ctctttattc tatttgactg ccattacttg caataacctt cttgtaccaa
    14881 taaaacgatt ttttcttgta acgcttgagt gttccctctc ctttattatt gcgatcaaca
    14941 taaatgaaac catagcgttt ttccatttca ccagttcctg cagatactaa atcaatacat
    15001 ccccatgtag tatagccaat tagattaaca ccatctacat atatagcatc tctcattgca
    15061 gcaatgtgct gacggaggta atcaatacga taatcatctt ctacgtaacc attctcatca
    15121 ggaatatcta ctgctccaag tccattttca acaataaaca tcggtttttg ataacgatcc
    15181 cagatagcat ttaaggtaat gcgtaatcca agcggatcaa tttgccatcc ccattcagaa
    15241 gatttcaagt aaggattctt aagagatgcg aaaatatttt cttgaacctg ttcactgaca
    15301 gttggattac ctgaagctac tcggcttgaa tagtaagaaa atgaaataaa atcaaccgta
    15361 tagtctctta acaaagcaag atcttctgct gtcatttgaa ttgaaatacc taaatgctca
    15421 aaatattttt tagcataatt agggtatttg ccacgcgctt gcacatcaat aaaaaaataa
    15481 ttttctctat ttttttgcat cgaagcccaa taatcttgtg gatgacacgt atttggatag
    15541 tattgacctg cagctaacat acatccgatt tgactatttg gatcaacttc atgagcaatt
    15601 tttactgcaa tagccgaagc cactaactca tggtgagctg cttgatattt aatttgctct
    15661 tgattttcac catcttcaaa atataagcca gcgcccataa aaggagcgtg taaaatcata
    15721 ttgatttcgt taaaggtcaa ccaatattta actaacccct taaaacgagt aaataaagct
    15781 cgtacaaggc gctcataaaa accaatcatt ttacggctcc gccacccacc gtaatgttta
    15841 ataaggtaga taggacagtc aaaatgtgtg atggttacca aaggttcaat accatactta
    15901 cgacactctt taaaaatatt ttcataaaac cgaagaccag cttcatttgg atatagctca
    15961 tcaccctttg gaaaaatacg cgtccatgct attgacatac gataggtttt gaaacccatt
    16021 tctgcaagta atgcaaggtc ttctttataa tggtgataaa aatctattga ttcttttgct
    16081 ggataaaagt atccttcctc aaaatcaaac attttctttt gtcctgaaat aattgcaaat
    16141 cgatcttctc cagtaggaac aacatctaca tttgctaggc cacgtccatc aacattgtaa
    16201 gctccttcac attgattggc agcagttgcc ccgccccata aaaaaccttt tggaaatact
    16261 tgttttacca tcatttaccc ctcttgtttt ttcttatcat aatattaaaa aagaccagtt
    16321 actgacattg attcgccaaa tactggtact atccgtgaca tgaactttct acttagaagg
    16381 tcttttcact aattctgtgt atttaaatac ttaaaaatag tatctaaaca cttttaaagg
    16441 tggagtttta tcgtttcaga aattttagca taggcttgat ctgttaggtg caaaccatct
    16501 tttgtaaaac tgctagcaag ctgacctttt tcgtcaacta ataaatcata caattcaata
    16561 taattaatgc cagaaattac tgataaggtt ttgtttaaag cttttattgc ttgattattt
    16621 cttaccttga cacgctctat atagtcatct tcttcactaa caggtaaaac actcagtata
    16681 ttaatctctg tcatataaga ttccgcacga atttctgcaa ttatatccgt gatattagct
    16741 atgatttctg actgtgaatg ccctaaacca atatcatttg ttccaattaa aataaatact
    16801 ttggatggta atagttccca tacttgggtt ctcaagtttt ctaaaagcca ataagaatct
    16861 gatcctgcta ctcctctatt aactaaagga tagtcacgtc ctaaatgttt ttttaaagga
    16921 aaaaactcaa cgatagaatc tcctgtgaaa acaatggcac cttcttgtac tgtctgattg
    16981 ttaatctcaa aaaactgttc tcgtttcatt tgataatctc ttaaagcttt gtcaataact
    17041 tctaacatcg ttctccttca ttcttaaata tttctctagg ctttcttgta ttataacaaa
    17101 atttcacctg agaattgaat agtatgcttc gtgaaaagct acgtggttct attcattatc
    17161 actcatcttg tgaagaaagt tctctatcgt ttctaacaca ccatcttgat cgttactaac
    17221 tgtttgatat ttagcaatag ctttaatttc ttctggcgca tttgacatag cgtaagaaat
    17281 atttgctgct ctcaacattt ccacatcatt gcccccatca ccaaaaacca tcacttgatc
    17341 ctgtttcaat ccccactttt caaggagaat agcgatacca tccgctttat tgacatcagc
    17401 tggtaagaca tcgatacaac caaaaccact agaggtcaat ctgatatgat gatttttaaa
    17461 gtgttcacta atatctaaca acatctcaga aacaagattt tcatttaccc aaagtgtcat
    17521 ttgaaaatat cggtcatcat ctaagggcag caaatggtct ttatagataa aattaggcat
    17581 atagtattct aattcagtcc gcctattaat aggagttctt cgctcaaggt atgaacgttt
    17641 ttcaccagat aagctgatga gtgtctcagg atagttctga tataaatagg ataaaacagc
    17701 tgcaactgta tcattagata aataagtttc ttttagtaat gtgccatctt ggtcaatgac
    17761 acgaccacca ttttcagcga cgtaggcgat acgattaggg tattccaaaa agtttaacaa
    17821 caattgatcg tataaattac cactagcagc tacaaattta atatcatact tctccatcaa
    17881 cgttaataaa gaagaaaacc gtgctttatc ataagtttta tctgagcgta gaaaagttcc
    17941 atccatatct gttgctatca atttaatcat acaatctaac catttccctt tcaagggggt
    18001 gcaaaaccca agtggattgt gcaactcaca ttctactaca acttctctca ttataccata
    18061 gaaacctcaa aattgacacg cctaatctgc tataaatcaa tccgtaagcc atttttaaag
    18121 gttaggaaaa ttttcttgtc ttctttgata tccaatcgct caacgctggt ctgccagagt
    18181 tcttggttga agtctgtgat gaggtcatcc tgctctttaa aattttggat aaaaccatca
    18241 agactgagct gcttactttt ctgctcttcc aagactgacc tagtttcctg caactctttc
    18301 tgtttttctt gataggctgt taccaaggta ttgtactgtt cttggtagag gtcttgattc
    18361 tgagctttcc ttgcattcct atcgaccaag tcctcgatgt cttgtcgtat ggtttcaaga
    18421 tgatgctcca agtcatcaag cctagcttca agtgacgaag tgtcttttgc catgtcaatc
    18481 aagagttccg tattagcgat aatctcatct cggttactga ggagttgatt gatagctgat
    18541 aggaaccaac ctttaatctc ttcctccgtc acatgaggtg tctgacattt atgctcaccc
    18601 ttgtacttgg cattgcactg gtagatagtg cgtttgtact tgctggtaga gtgccacacc
    18661 ttactgccaa aagctgaccc acaatcaccg cagaatagtc gtcctgtgaa aatgttgctg
    18721 gtttgccgtt tcctttccag cttatccaat tcaacctgaa ccaaatcaaa gacttcacgt
    18781 ttgataatgg cttcatggtt gttctccaca taatactgtg gaagttcccc ttcattgagt
    18841 ttagtgcttt ttgtcaagaa gtccgttgta aaacttttct gaaggagggc atcccctttg
    18901 tatttttcat tccgaagcat acgcttgacc gtgccatagc tccactgcct tttcccttgt
    18961 ggcgttggaa tcccttgggc agtcaactct cttgcaattt tgttggggtt gtttcccaat
    19021 aaaacttgat gaaaaatata cctaacgatt ttagcttctt cgtggtcaat ttcaaaacca
    19081 cccgtgtcac ttttcttgaa gcctagaact tggctgtagg aaaaggtcac ttgcccttca
    19141 gccaactggc gtcgtctacc ccaagtcacg ttttctgaaa tggaacggct ctcttcttgg
    19201 gcaagactcg acatgatggt aatcagaagc tcccctttgg aatcaaaggt ccaaatgttc
    19261 tccttttcaa aatagatttc aacgcctgct tccttgagct tgcgaaccgt tgagagggag
    19321 tctaccgtgt ttctggcaaa tctactgact gacttggtta aaataaggtc tatctttccg
    19381 tcaagggcat cttccaccat ttcttgaaag ccaagtctct ttttggtgtt tgttccagaa
    19441 atcccttcat cggaatacat cttgacaaat tcccaatccg agcgactgtt gatgtagtct
    19501 gtgtagtatg tcatctgtgc ttcataagaa gttgtttggt cttcatggtc agttgacacc
    19561 ctggcatagc ctgccactcg tcgtttggta aatctgggaa gttcaacctt atgggtgact
    19621 tgtttggctg gttctatcgt gataactttt ttcatgtcta ccgtcctttc tgaatgatga
    19681 cgtgtttttc ttctccattt ctatagatcg ctttagcttc attggtcacg ctattaaaag
    19741 aaatatggtg aatgtcttct gtatttatgc tctcaccaaa agcttgaaag agctgttctt
    19801 ctgtaacggt acgtcctaaa caagtctcaa cacctttggc atctcttgtc ctgcaataat
    19861 acctcactcg cttttcctga ttaaatttgg tctccaaagt cagaaccatg tctaaaccgc
    19921 agtgctcaca atacactttg ccttgtaact ttgctagggc atcatgcttt gagactctcc
    19981 tcctagcacg acgctttttc tcttgattga ccagttggaa atactccggt gtcacaatag
    20041 cttcatgggc attctcaacg atatacttcg ccatctgccc atcatttaga atggagcgac
    20101 tgccaaattg atctcgaaag gtcttctgta aaatcagtcg tccacagtag gcttcttgat
    20161 caaacatgtt atagatgact cgtttagtga agcgattccc tagccttgtt cgctcacccc
    20221 tatcatcaag ccttttagct attttagggg ctgaaagtcc ttccatatac cattcgaaga
    20281 cctgcctgac aatagaggct tcatggggtt caatcacata ctcgtcgcca ttccaccgat
    20341 agccaaagat gtcttgagga atataaggtt tcccttcttc aaagcgtttc ttcactcgcc
    20401 atctgatgtt ttgactgata gactgtgatt cttcttgagc catggaggca agcagggtta
    20461 aaagcaactc cccttcagcg gttagggtgt caatcttctc cttttcaaag cgaacactga
    20521 taccaagtcg cttcagctca cgaacagttt ccaaaagctc caccgtattt cgcccaaagc
    20581 gtgaaataga cttggttaag ataaggtcaa tcttaccctt acgacaatct tcaagtaact
    20641 gttgaaagtc tcgtctataa gcttgactgc gaccactaat ggctgaatcg ctgtaaactc
    20701 ccacatagtc ccattcagga tttgcctgta tcaattggct gtagtggctg acttgattgg
    20761 ataaggactg gagtaaactc gtatgcgaaa ccctagtgta tgcggccacc tttaaccttt
    20821 taatggcagt taccttctgg gcttgtatcg ttttgatttg tttcattgat acatctcctt
    20881 tcgctattat atatcactct aaagccctta tttatcaagt ctttaggcca ctaattggct
    20941 cataaaaggc tcatattttt cgagcatttt tgccttaaat tcttggaaga tggcttcaga
    21001 aatcagaccc tgggacagga gctgctttgc ctgtgccata gtcagttggt aggtaagttc
    21061 ttgttgaaag tcttgttctg tcatttcttg tctccaaatc tagcagtcac atagcactta
    21121 cgactacaat attttctctt aggattagcg taggaggtaa actcaccccc acaagccaaa
    21181 cagacatgct cggtataggc ctgtcgattg acttcctcca aatggctgtt ccaccatgtt
    21241 tgacggcagg gtgttccaca aaagcgtttc tttttcttac cgtctatatg cgtcaaaaca
    21301 cgaccacaag catgacaata atctggtaaa agctcatctg ttttctcacc agcctcaatc
    21361 acctcacgac gacaaaatga ctttatggtg ttagacgata gactgagctt tacgccaatg
    21421 gctttataac caagtccatg ttctcgtaaa taacgaatag ctgctttttg ttctggggtc
    21481 atgactttcc tccttctacc tactagggga agatggtgag gatttagtcc aacttttgaa
    21541 gaaaaaaagc aaaaagaaaa agcctaatgt ctccaccagg cttaaaatct tttatcctaa
    21601 tctgaaccaa ccgataactt ttccaagttt gacggtgcca gtcgcatcat aaagtgagcc
    21661 atctgccatc cagacacgct tcactcgacg ggtaataccg ccaccaccaa tttccagttg
    21721 gtcattgata ccgttcttgt tgtggtcaga gtagccatcg acattctgct caaccccatc
    21781 aatgcttgta ccatctgaat ctgtgataca aatgccgatg tgaccataag gatgactgtc
    21841 tgtcttaata acatagaagt caccagcttt agggttaacg ccccaagcgt ccttaatgac
    21901 tgtaaagccg ttagctttcg ccttggttaa acaatcgatg gcattggtgt atgccatgtc
    21961 cttgtcggtc aactcctgca caatcttatc caccagactg acgcattgtc caccataagg
    22021 gttggtagga acagtcactt tctgaccaac tttagacagt gctgaagcaa ccgcgcgttg
    22081 ggcaagactt gttgctactg ctttgatgga actcgtcgcc ttatggactt tcaaggtttg
    22141 accaatcttt aagacatcgg ttttcttcaa accattcaag gctaaaagac catcaaccgt
    22201 tgtcccagat tttcgtgcaa ttcgccaata gctatctccc ttttgagccg tataggtctg
    22261 ttctgaatga ccaaggctgg ttccttccac atcctgttcc agtacccaag atgtaatccc
    22321 ttcaagtaag taggctctct tactacgaga ttggtttacg gctttgactt ggaggatttt
    22381 gtaggtgcga cctttgaccc aacttgccat cgcttgaccc gtttggtaat gagtcgcatg
    22441 accaagcaca cgaacactat ccccaacgtg ataaacaggg ctggtagaag catggggctg
    22501 tcctccttgc ttactggggg tggcagagac aactgtcttc acctcctctt gggtaatagc
    22561 agagacgaga cctttggcca gttcttcctt cttattttca aagatagtca tgtcctcttc
    22621 gttatcaata aaggcaatct ccaccaagcg gtaagtgtac ccacggttct tagcttcgtt
    22681 ggcattatag agccaatcca ctttcttaat cccacggttt tggaaatgac ggctaaggac
    22741 agatagaata gccaaatcct ccttatcagc cgtcaaagaa gattgaatga ggacttccgt
    22801 tcctcgtgca gtgccattaa aggcattgaa atgaagctcg gtaacagagt catagccctt
    22861 accaaggatt gccagtgact tataatcata aacattttgg tctgtgatat aatcgatgtt
    22921 tttgccgctg tacttggaca tgagtctagc caactcacga acctttccgg cttctgtgat
    22981 gccacgttta ctattcactg ctcctggatc ataggtagtg cgtccttgcc catgtccaca
    23041 aatcactaga tgttttccca tgttagtctc cttctttttc gttgaattgt tttaagagtg
    23101 cttgcagttt ctcagggata ggtaaaccaa tccgcaccgc attttccaaa atactcaagc
    23161 cttcattact caagtaaaag aaaatcacca tggtgcgaat cgctccaccc tgcttgatga
    23221 tttcagtatc aatgaggtgt cccacagata ccaaaaagag aatggctatc tttttgaaga
    23281 tacccttaaa tccaatattg ctagcaagcc ttttctcaac tgctgctgcc ataagacctg
    23341 tgatgtaatc gatggcaata aaaatcatca aagcgaagaa aaagccatca acttctccaa
    23401 aaatcgaacc gataagccct ccaatcgctg agaagagaac tttgtttgtt gccagtaatt
    23461 ctttcataat gacactttcc tttcttatgc tgtcctacgc caacggtaaa ccgtgacgta
    23521 aggttgtaag ttgttgtgtg gcttgttgcc accagtattt cctgtgttat tcccttgagg
    23581 gtaaagacta gagttcccat ctgaagagta gtctcgtcga atagcctgat tgccattatt
    23641 ggcagataca tactgagcgt gcgagtgaga cggcatttca tcaaccgtca aggtatgtgt
    23701 tttactaccg cctgatttat taacgctctt aaactcactt tcattctcag atacgccaac
    23761 taagaccctg ccattgccaa acctctccca agtcccaccc ataatggtag ctggactggt
    23821 acttgacgtc gactcgtaaa tcacaccgac tgggtaaaag atatcaagaa gtttcttatt
    23881 cttcatataa atctcaccgt caaaataagc aggtaaactc ccatccacat caagtacccc
    23941 tcttgtccac gctttgccta tccccatacc agatggactt aagccgtaga cgaccttctc
    24001 tggaccaacg gtaaattcaa aggtcgtggc ataaaagagg tctgcgagct ttccaataat
    24061 gttgtaggat tttgtcgtat cgtaggtgcc ccctaaaata gcttggaaat ccgtctttgt
    24121 gtgttccgtg gttgacgtcc agttggccgc accaccagcg tttgtgacct tctggccact
    24181 tgccaaatca accacttccc atgtcaaggt tgccttattc ttttgaacac tattgatggt
    24241 tagaggggca atcttaagct ttcgtgtgac cgtcacctga ttcatactag aaccggcacg
    24301 aactgctgaa aatgaaaaga taggtttgaa atactctaat agggttatct ccacttcttt
    24361 tcgagcactt tgtcgccctc tcgaatcagt cacataggca gagaccttag cgcgaccaat
    24421 ccagttgata cctcccaaaa gaccgttatt actcgttacc acattaggca gttgaaccca
    24481 ctgattattc tcaaatttaa agacctctgc acgataaccc gttgaaggaa tgatcgatcc
    24541 gtaaacaccc gctccttgat taaaagtcac tttagggtta gacactaact gggcaaaact
    24601 tgtactagtt aaaatcgttt ttgcagtggc atgggattct gaaacagaaa tactacccaa
    24661 ggttggaaca actgacgtcg gtaggttgag ggtaatggga attgtcatag aacctattgt
    24721 cttatcacca tagatagtag ctagcgttaa atgaccgtta cctgaggtgt tatttggaat
    24781 ttgagttgct aattgcgata ttgatggcgt ccaagttaca gaggtcgcaa tgcctgttcc
    24841 aatggtgcca cttagagatc caaaatgcca tgtgatatta tgggtaaaat cactactagc
    24901 gcgcttaatc gtaatggtta cgactttccc catcatattc ccagaaactg tagcactcga
    24961 tgaccgtgga atatcactta aacgtagcgt ttgtgaacct gtattcaagg tgccaggcga
    25021 ccagccacca gacccagtga aggtcgctga aaaactgatg gtttttgacc cattcgaatc
    25081 atgtgacaca gtgatggtct tatcaatcaa gtgaagagaa ctatgagcag tatacatatc
    25141 tggtcgccct gaccaagaaa gcgtctggcc attgatagac acacttgccc tacagtcata
    25201 catcccaaag gtcgtataac cattctttag ccaaagttgg actcggacag tagatgtatt
    25261 attagccgtt gaagttcctg tttcttccac tcgtaaaagt agggtatagc ccctatcatt
    25321 atttgaacca taatctgcca taggatctcc tttctacttg gcatcaataa aacgacaaac
    25381 tagatgcttg gcattatgcc ttgcggcttc tagtcggtaa taaccaacct gtaaggtctc
    25441 cacaaaaacc ccatgatgaa ttttaatgac accagctgta accgtcatga cggcattacc
    25501 agctgactta atcatcatcc cttgtgaggt taattcgata tattcagagt tatccttctt
    25561 accaataatc accccattat caccagctct cagataagta ttgacaaagt taagcaatag
    25621 actgttggct ttaagatcgg cttcaatcgc tgcgatacga gctgtattat caataaagtc
    25681 ttgattaaat tgcgcaagga cagcttcatt attcttctca aactctttat aagacttgag
    25741 ccaatcagct acttcttttg ccaaagctct cgcttcaagg tccactcgga gagattctgt
    25801 cttttcagtt agaaggtcta attgctgctt cataaacccg ttgtcagctt tggtatcaat
    25861 ctgagcgata agatcattca aagaaggccc aggcgctgaa gcaacgttgc catcttccaa
    25921 ttgaacattt ctaagataaa ccacatctcc tactgaccac gaacctgact ttaagtaaaa
    25981 gatataagaa tagtactgat aactacttac tttccatgaa gaaacaaacc tttgccaatc
    26041 tgttgttact tcgtaagctt tcgttccacc gagttcacaa cccatattga gtgtgacaga
    26101 ttttgaacac ttaatatcaa ttgaaaaagt catagtcgca ccaattcgac tccttaaatc
    26161 atagaagttc cgatgaaatc cagctgtacc tgctttggta caggtcatct ttacagtcac
    26221 gccactaaca gaactcgtat cctcaaccac ctccttcttc cactcagagg ttactgatga
    26281 aaaggtcaca gccttcatgg cgtaatcgtc aatgtaatta cgacctccca gttccgttcc
    26341 ctcaaaaaag gaagaccagg tatagtcact aggattggtt gaaggcgtag cactctccct
    26401 attgacagct agtccgaggt agcgtttgcc agttgagacg gcagaaatcc catctccctt
    26461 gtcactatct gcgtacatgc gccaagtgta tagggtcttt ccatccttac cagcctttcc
    26521 atccacaccc ttatccccat aaactccgat gacaattggc gttgttaccg ttgttgagcc
    26581 attagtaaac gtggtttttt catagttcca cagatactta agcgttgagg tgagtgaggg
    26641 aatagttttg ctccaaccag agcttgttgt ggtaatacca gtcttttgtg cagagactaa
    26701 gtagtactgc tcccttaatt gaatgcccac cccatctgct ccggcgtccc cctttggtcc
    26761 aggggttaaa gagatagttt taagatcaac ttttgtcgct aggctgtccc ctctcacctt
    26821 taggagtggc gtatcaattg aaagactccc atccctatcc aaactaaaga ctggggtgtg
    26881 ctggccagga ataacaacct tgtctgcttc aatttctagg ctcttcacat actctgatag
    26941 aatctgttga gagacaagcc ttgtcatcca tgctgaacta gagacggtca actcatcaat
    27001 cgtagctttt tggagtgctg cgactcgtgc ttcaagggca tccgttgcca gataatcaag
    27061 ggcagctttc ttcccagaac gctcaccttc tgctttggcc ttagcgatac catcttcaac
    27121 ttctgtttga agacgctcaa agtgcctatc aaagaccttc ttaaaattag ctcgctcctt
    27181 agacgcacgg tgttctgtag ccgactgatt gacatcaaga atggtcttag ccgcactggt
    27241 tagcgaattg tttcctgatg atccagaggt tgtaaagcta acctcatcat caaaggtcac
    27301 caaaagatac acttcttcta gagcatcaaa ggtataacta actgcctttt tcttaacatc
    27361 taccttgtgc ttttgacttt taagagtgac tgtatctccc agatggactt cttgaccatc
    27421 aagctgataa gcttcaacgg ttatctgtct agagatgcta tcgatgtgct catgcgtgaa
    27481 cttagccatc gcccattggc gcaattcttc ctctgtctga agcgtgttat tctcataccg
    27541 tgcttcgtgg acataagggt attggttaat gaggggactt tccacaacga cagaaagaac
    27601 ggtatcctca tcactacctt ctatctgaaa ggttgaggtc gcatagatgc gcgtaatgac
    27661 cttctcagaa tcacccttat cctcaaaagc tttcagattg tgatggcttg tgagaataac
    27721 tcccttatcg ttgccacggt gtttcttaac tatcagctga aagttatcac gaacgagctc
    27781 accttcccag gttccaagga tggaatgttt accatccatt agagcttgat aaagtgtcaa
    27841 gtcttcgtca gacacatagg tatgacgctc cgtcacatca ctgtcaaagc taaaaagtcc
    27901 taaatcagat ggacaaacct caaccatcct cataagggct gattggcagg tggtattagt
    27961 cgcagaaaag ggcttaattt gacgtttcat cacatcatca gaaatgtgag agcactcaag
    28021 ctctacccta tcgtcttgaa tttttgcctg cttaacacga aagagctgat tccccaaatc
    28081 aggagttgga cacaaaatca actcgtctgc tctaagggtt tcatgaacac ctgaatccgt
    28141 aatggggtaa gttaatcgta gctgaaagat accatttagc tcctcttcga ccgttgcact
    28201 caccgtttca aataatggct gaccattcca tttcgctgtc tttgtttgac catcaagaag
    28261 gtaaagcatc acacccaccc ccaattcgtc tcaaaggtaa gcgatagtat cccactgcct
    28321 aaaatcactc cgacagattg tgttggatga ctagcatcaa tcgtgatgaa gtccccagac
    28381 cacttgacag ctttccctga tagggtctta aaactaggac ggtctgggtg attcaccata
    28441 acaagaggct ctgtaaattt ctcaatgcga atcacttggt cccctactgt aaacgaagtc
    28501 tctgatacgg attgaccagt tatggtaatg ctaggaaagg caagggcaga tccttgtgtt
    28561 tttaacacac cattggcact caaaacttgc ctatccacac tcttaaaaaa gcgagtgggg
    28621 tgacagataa aggtcacatc aatcacatac acatcatggt catcctgctt gatgtcaaag
    28681 ctatccgttc ggtaacacca gagcctggtg agcttaaggc gttcactttc tagccaaaac
    28741 ccttcctgca tgagataagc cgagaactca ttgacccctt tctcactcgc accaatcaga
    28801 tacaagcgat aaggtttctc aatcacttca cgatgcttat tggtttgaac aatcgccccg
    28861 ctcaatccac gatggtctag gagttgcgtt ttagaccgtg gcacttgaat gctcggtcta
    28921 tcttccacaa gaactttaaa aagaaaagac gaggttcctt ttccattcag taccaattca
    28981 ttatgtttaa tcacactgtt cctcctctca gtaaggcttg tcgattcatt tcatcagcta
    29041 atcgaccagc cacataatcg gctagttttt tcatatccgc ttcttcacga atcacaacat
    29101 ccgtgatatt gactgttatg cttgttccct tgttaggcat ggtggctgcg atgctgcgtc
    29161 cgatactgcc aagagtttgg ttattaagag gtagaactgc ttcacgtcct gcctctcctc
    29221 caaccatcaa gctatcccct gtcataccaa atgccgttgg cttagtcaga attccaccct
    29281 tggcgtacca ctgaatggaa atcttaggta agccaccttt taaccaatca aggggattgg
    29341 cagaacctga cacactaaag tgcggaagag gaatatgcgg ccacttaatc ttgaaattaa
    29401 agagattctt aatggcattg atggctgaag aaaccgcatt ttttgcccca ttgatagcaa
    29461 ttgtaatggt cgatttgacc ccattccaga cagaggacac tgtgtttgaa ataccattta
    29521 ggacacctga gatagtagcc ttcattccgt tccaaacgga agataccgtt gagccaatac
    29581 tagatagaat ggaactaatc gtggactcaa tgccattcca gacattacta acaacaccct
    29641 tgatactatt gagtaagttt gtcatggtgc ctttgatacc attccaggaa ttggaaatga
    29701 actgcgcaat ggcatttaga acaatcgaaa tcagtgactt gatggcttcc caaactgtag
    29761 agacgacctg cttgatggtt tcccaggcac ctgaccaatc acccgtgata atctgcataa
    29821 ctgccttgat aatacctaag acaacattga tggctgtttc aacgaccact ttaatgatgt
    29881 cccaagctgt cgtgataatc agtttgatat tctcccaact ggcttgaagg taaggtccaa
    29941 gaatggtcat gatggtttga atgactgttg aaatagctgt ccacacggta tttgcggcat
    30001 taaggattag ctgttggttc tctgtccacc aagtagtcaa ggttccccat atcgacataa
    30061 caaagcttga tatctgttgg ataatgacag ataaaaaggc atagatggca ttccagattt
    30121 ctgtcacagc tgtccgaaag ccttcgtgat gttgccaaag ttgttgaatc ccaacaacca
    30181 gtaaggcaat agcggcaata acaccaagaa taatccctac gattggagct gctgcagtta
    30241 ccacccccat aatggttgtc cccatagcca tagtggccgc ttgcagggca atgaaaatcg
    30301 gtaagactaa accaagagct gcgaccaaac ttccgacgat gacaataaac tgcttgactg
    30361 gctctgatag cccagaaaac catgtggcaa cagcttgaag taaactcgct aatatttcca
    30421 agataggagc tagggttgcg gcaatagcgt ctcctatttc agccatcgct aacttcgccg
    30481 tgttttgagc ggttgtaaac tgatcaatag gatcaagcgt cccctcataa gtttgcgtta
    30541 caatcccagc tgccttatca gctgttcctg ctaaatcttc aaaagacaaa gcaccacgct
    30601 tgatggcatc aaccatacga ggagctgctt tacttccgaa gatttctgag gcaagagaaa
    30661 gagcctctgt ttcactggtt gaagttttga tttgctcaat ggttccagca agtccatctt
    30721 gaagcgtaag cccatcgccc gcatacttaa cagctgcctt tgagagtgac gagagtgctg
    30781 cagaagaatc aacccctgct ttttcaaact gtcccatcaa ggtgacgccc tcatcaaagg
    30841 aaaggccaag ggctttgatt tgtggtgctc ccactactgc cttgtccatc aactcttgga
    30901 caccaacacc tgttgcttgg ctggtataag taaccgtgtc taaaacactt gataaatcag
    30961 tcgcttcaag tccataggct tcaatcgctt gtttggctga aagggcagag ctcgtcacat
    31021 cactcccatt gatctctgag aactgaatca actgggtaga ggctgattta agagcatctc
    31081 ctgttaaccc aaattgcgta ttcaactccc ctacggcact tcctgccgta ttaaaatccg
    31141 ttggtagttc agtggctagg gttttagcga tgtctgtcat ctcttcaagg gcagaaccag
    31201 tcgctccagt ttttgtgaca atgatatcca taccctcatc aacttcaaga aatgcgtcaa
    31261 gcgattgttg accgaagtca atcaatttct gtgacaactc tcccagttgg tcgccaaact
    31321 ccatgagaag gtcagccttt aagaggctgt ttgtctcttc caaagaagcc ttggaactcg
    31381 cagagctaga ggccaactcc tccatctcat tttggagatt gttgtaagcg gtctttgtct
    31441 cattaagagt tttctcaagc ttgttagctt caattgaatt ctccccatac tcgctctttg
    31501 tcaaagagag ttgttgttcc agattatgaa tctgtttctc aaggatttct gaatgagaag
    31561 cgaccttttg ttcggcaagt gctaacttat cagcctcact tgcagtcgaa cctaaagccg
    31621 attcttgtag cttaaaggaa ctattgagtt tttcgctttc tgacaccaac tgtgcctgct
    31681 cattttggag acgattgagc ttggactgat tggtttcaac ctgcgctccg ttttctgaga
    31741 gagctctgtt aacactttct agctttgatt catagccctt taagactgtt tgagtactct
    31801 ccacctcacg ttggaaggca cggtattggt ctgcaccgat gttaccggcc ttgaactgag
    31861 cctcgacttg ggcttgagct tgacgaagtg tggcgagttt ttctttagtt gtttcaactt
    31921 gtttggctaa gacttcctgc ttttgggtca aaagagtgac attgccagta tcaaacttaa
    31981 gagccttgtc aatctgacgc agttctttgg tggcttcaga agcctgtttg ttcacaccct
    32041 ttaaggcatt ttgtaagggc tgggtgtcgc caccgatttc aatggtgatc cccttaatgt
    32101 ttcctgccat cgtcactcct cctccctact agaaattgtc aaagtctgct tgggtagctt
    32161 tgcgaacacc agtctcatct cggcttctta gctccacata atccgtctga taatccagtg
    32221 ccatgccaat tgagatgtgc tttaagtcat caatggaaag gccagtctcc ttacaacaag
    32281 agaggtaact ctctaccgtg aagatttcct cgctcgctgt ttcggaagtt tctgcttttt
    32341 tctggttgtc atcccttggt taagcatgga catcaagact ggcccaactt cctgaagggg
    32401 aaattcctcc atcgacataa agaaatcctc gaatggtttg attcgaggat tggctgactt
    32461 ggcaaagacc caaaagagac ggtggaaaaa ggtcatgtcg aaatcagaca agatagatag
    32521 gtcaatctga ctagccttta actcttcccc ctcttccaat tgctcaagct gtgtcatgat
    32581 ggattccgca cttaacatgt taaagagatc ctggaaataa tcttttccaa attgttcctt
    32641 ataagcaatt ggggtgtagg cattcgtagc caagggatag gttttcccag caatggtgat
    32701 gttttgtcgc atgttctcct cctttaagca gctggttcaa agacagactt aaaccagttc
    32761 tcacgaatct catcacttgt ttcttcagtc gttctgcggc gaacaatctt atcaagtggg
    32821 cgtggacttg ccgtaaaggt caactctacc tcattgatat cagacccaga cttggtttta
    32881 gagcctacag ttgggcgtga agcgtaacag taataaagca catgaagcgt ctctttcttg
    32941 tccccttcaa atcggaacat gagagcgaag ttctttttct tgctgctggc aatctctgaa
    33001 atggtattgg tggtcacatc aagttgctcg cccaaaacac gagtcagaaa ctcctgagac
    33061 aagagggcaa ctttgagtgt tccctcatag ccgtcattgg attcagaggt gtaaaagttg
    33121 atgttgtctg ccttgtaaga acccttgtct ccagttggtt caagggttag ttctgcagca
    33181 ccacgaaggc gctcgacagt accataagtc aaagcaccgg cagctccctc actggtgact
    33241 tctgcccagt ggacatcttg taggccaaag gtgaccttgt ttttttctgc catggttatc
    33301 ctcctaatag tgtgatggaa taaatggttt ggtagagttt ctcactagtg atgtaagtct
    33361 ctaccttgtc aaaataaaga cggtgggcat caagaactga ttccaccgtc ttttctgttg
    33421 ctaaatcttt ctttgtcgtg taaagctcaa tctggacgtt gagccttttg tgataagtcc
    33481 agttatcggc cccaagatta tctgaatcag tgaccagata aaccataaag ggcggactgg
    33541 ggctgtgccc ttcctcaaaa tggtgatagg ctactgggag tttggttgct ttcaagacac
    33601 ggaaaagctc ttcaaatctc atagaccacc tcacagtttc tgtcgcaatt tgtcttcaaa
    33661 cgactgaatt gcctttttct cgacaggagc gatgtgcttt cgcccttcaa cccgaccacc
    33721 attttgtttg gcatgcccat cttcaagcag atgagttagc ccaggcgttc ggttatgaat
    33781 ggttttagtc agatcggtat tggtgtcagt cgttgtctta ctcgtccacc ctttagcata
    33841 tttccctcgt cgttttgggg aagtaacctt taaggtatca acggcatcgt ctgtcacttc
    33901 ctcaaccacc tcacgcatga catctgtggt ctctttggca taagtcgtca gctccttttc
    33961 tatgacagaa gctaaatcat caagtccaat cttagtcata aagctcctcc ttagtcgtaa
    34021 cgatataaat caagctccgt ggcaccgtat caccatcaat agactctatg gcataaaatt
    34081 gcccacgaaa gaaaatccga gtcgttaaag aattaaggac aagaaccttc ttgtcataac
    34141 gcaaggtaaa ctgcaccttg ttatgaatca gttttgtcgc actcccatca ctttcagtta
    34201 aagctagtgg acgacaagaa caccaacgca taaagaggtc atcccaaatg gccgactcgt
    34261 ttccgatgtc gtcctgcttg agtcgctttt cttgaaagac caactgttct cttagaggcg
    34321 caatcttcat cagaacacat cctttctatc tgccaaaagc aaatgataga gggtttcctt
    34381 caactccttg tgattggctt cttcacggtg ttcataaaga taggcaaccc catagaggat
    34441 tgccgtcttt agaacttctg aagtggatgt ctcacgaagg atatcttcac agagttggcg
    34501 gctagttgtc atcaactgct cgattagata gtcctcctcg ccattttcca ctttcagata
    34561 gagcttgact tcttctaacg tcatcatgcc gttttacctt tgatggtcaa agtcttcacc
    34621 gcttctggta gaacgagttt cccatccaca cgctggctgg caagaaaacc aatctgacca
    34681 ttgttagcgt aaagctcatt gagacgcttg aaggtacgcc cttgacggtc cgcaatccag
    34741 tagtaagaga aatcaccaaa tgcaatagct ttgtttcctg cttctggaag tggcgcaaaa
    34801 gttgacgtgt agtaaggacg gttgagaatc aaatcaggtt gaccagcctg tgtggacggt
    34861 tgccagatgt agtttccgtt attgtccttg agcttacgga ttgctttaac cgtagtatcg
    34921 tggagaatcc agactgcatt cttacggtaa ggagcaggaa gcgagtggta aagctcaatc
    34981 atgtcatcaa aggtgatgtc tttggttgcg gtcgttggtc cttctacgtc tgcttgagta
    35041 aagatacctg ttggtttttt tgaaccatca cccaccaaga atgatttttc ttcttctgta
    35101 ccgatgcggc gtgcaaactc agaagtcatg taagactcaa ggtcaaagac agagtcattg
    35161 agcaattctt cagagatacg gattgctgtc ccaatcttat gtgaatcaag agtcacttga
    35221 ccaaaagtct catccgtctc tggatagagc ccattctcgt ccatccaaga ggcagacccg
    35281 tgacccgtaa caactggaat cttacgctca ccactagagg ttttgataac agttgccagg
    35341 ctacggaaaa agttttcttc ttggagaccc tgaatcaact ttttctcgta ttcatcaggg
    35401 acaaggtgtc cgccttctgt gtcttcaccg acacgaagaa catccttcac gtcatagaag
    35461 ttacgcttac gaacactggt ccagaaagtc tgggtgtaga tgtctgatgc cacacctttc
    35521 ttttcatctt ctttttggtt atcgacaatg actgttggct gcgtcgttag agcttgtgag
    35581 gtaggttgcg ccagttcaag gtcaatcttt tcttggcgct ccaagcgagc aatttcctta
    35641 ttgtagagct cgattttaga ttccatctcc tcatagcgtt tggaatcttc atccgatacc
    35701 aagccgtctt cagagcgaac agtatccaga aaggctttcg cttgagccca agcagcgtta
    35761 cgtttttctt tcaattcaag tagtttagac ataggtattc tcctttattt caataggttc
    35821 aatcgttttt ccaactgatt gaaagggatt gttttctgtg gtttaggcgg ttgaaggctc
    35881 gcttgcagtt tcaccaccaa atcatgagca gcagtcactc tactaaaggt atagctattt
    35941 tgatgatcct gctcaggtgt ctcctctttc tcaaagagca ctttatccgc aaaaccaagc
    36001 tccacagctt tcttggcatt gaaccaagat tccgaatcca taagatgaga aatcttggtt
    36061 ctggaaagtc cagttctaag ctcataggca ttgataatgg actccttaat ttcgccaagc
    36121 atctcaatga ccttggccat atctttcgct tcaccttgcg caaacgtcca agggttatga
    36181 atcatcatca tggcaactgg actcatggaa actgttgtcc ctgccatggc gatgacactg
    36241 gcagcactgg cggctagacc atcaatgatg acatggacat cgccctgata atccataagc
    36301 atgttataga tttgagcggc cgcaaataca tcaccccctg gactgtttat ccagagggtg
    36361 atgtcgcctg ttcctgctaa caaatcattc ttaaagagct gcggggtgac ttcatcccca
    36421 aaccaagtct cgtccgcaat ctgtccctca atccgaaggg tgcggacttc tccttcgtca
    36481 gtaaaattcc aaaatttacg catcttcttc ctcctctggt gggccttcag ctggttccgt
    36541 ttctgtcggt tgcttcatga aaccaccagc atcttttaat ttggtcatgt ttccgttaat
    36601 caagtagagg tttccgcctt cttcatcaga gagcaagttc aagtcttcta actcacgaat
    36661 gtcattagtc gaaagccagc cattttgccg tgcgatggca tagccattca tacgactttg
    36721 gtagtctcca cgaagcaaac cgtctacatt aaacttgata agataacgct tcttttcttc
    36781 aggtaaaaaa agagacctct tgaaggcctg ctctaaccga actacccaag ggtctaaagt
    36841 atatttcaca aattcaagtg actgctgttc gatatttgaa aaagacgact tctccaaatc
    36901 cccaaccata tggggtggaa tgcggtaaag ccgtgcaatt tcgttaatct gaaacttccg
    36961 tgtctgtaga aactgggctt cttctggtgg aataccgact tgggtatact tcatcccttc
    37021 ttcaagaact gccaccttat gggcgttggt cgccccatta tagacagcat tccatgaatc
    37081 acgaactcgt ttaggatctt tcaaaatccc tgggtgttct aagacaccac ctgggttagc
    37141 accattttta aagaaggctg ccccataatt ttcggtcgct agtgtcatcc caatcgcatt
    37201 ttttgccatg gcaatcggtg agtaaccaat caagccatca aagccaagac caggcacatg
    37261 aagaatatcc tcctgcttca ataggactgt ccctttatct ttgaagttag gattctcttc
    37321 ggtttgcctt tggtatttgt agtagagttt cccactatca tcacggtgga cagacatctt
    37381 atctggcaaa agcggataga gactaatcac tcgtccagcc ttatccctga taatctgtac
    37441 ataagcattt ccccatatta acaagtgact cataatcgtc tctcgaaaga caaaggatga
    37501 catctcagga ttgggctcat catggagcag aaagtaaagt ggatgatcca tctttctctc
    37561 cttaccattg gttgtgcgtc cgtaaacatg tagagggaga cttgcgatag cttctgccaa
    37621 aatacgcaca caagcataaa ctgccgtcgt ctgcatggcc ttaaattcat ccacattctc
    37681 cccactggtt gtgcgaccaa agaggtaaga aaagtcctgt ccctcatagc tatttcgagc
    37741 tacaggctca gcccttgccc tttttagtcc tagtacatca agtaatccca taatccctcc
    37801 ttattttggg tacgaaaaaa gcacctccgc atttggagat gcttcatgct atattctttt
    37861 tagctttcca aaatagtctc aatcaaatct cgaattgacg tcaccgaaga gtgatgttcg
    37921 atatcttttt cttcaagtga ataagcataa acaccagtct cgtctgacca ataggccaca
    37981 gcatcattct ctgaaaattc ccctgcagaa actttatctc ttaactcggc tacgattttc
    38041 tcttgaattt ctttaatcgt catattaaaa cctcacttct taatagttct attttgactc
    38101 taaatcctaa ctttcggtca aaaaatcaat ggctatttat taaataattt agcccaattt
    38161 tgctttgaat tcataatacg agcaacgttg acggtgtgtt gctctataaa ataaaaagca
    38221 atatggtttt cgataggcat atagcgatag attttaccat catcagtcaa ttgaccataa
    38281 ccacgacttg ataccaaagg acaggcctca ggaaaagttt ctaaggtttc aagagcagat
    38341 aatattaaat caatcttccc atctgcagat tgctggctat aaaagttcac taagatatag
    38401 tcatggatat ccctcaagtc ttgctttgca tggtctgcaa gaagaacatg gtaacgtttg
    38461 tgattagtca aggccaaatt cctttctgac atcagagagc gcagtcacct tgccctccat
    38521 gatatcttga tgcccaagta aaatctcctt ttttaaatct tcaaaggcga tttgatactg
    38581 tgattctgtt aaatcgctag aaacgaattc ttttgcatcg actgcccctg ttgcaatttt
    38641 acggagagcc gcattgaaaa catcagaaag cgttaacttt tcatctgcta gaattgcttt
    38701 ggtttgttgg taaaaggcag aatctgctct aaaattgact ggttgagtat ttgccataat
    38761 aaagacctct tttgtaaaga tgatttgtat ttacatttta tcaaacttct ctttatactt
    38821 caactaaaaa ctcaatatcc ctcgctcatc atagacacta gtttcatcac cttggtggcg
    38881 aatacaacgg tcaagtccca tgataagtga cacaataccg tcaatcttct cgactgactt
    38941 ttccttgtct ggcttgatgt taccagcagg gtcttgtcgc atgactacat tctgtcccat
    39001 ccatttaaga acaggatgac caccgtgttg gattttccct tccatcatga gtttgtagag
    39061 ttccttggac ggtgggctca tatccttata gccctgtcca aaaggcacca tggttaagcc
    39121 cattccttca aggttctgca ccatctgggt cgcattccaa cggtcataag caatctcctt
    39181 gatgtggtag gtttcagaga gttgttcaat aaaggcttcg ataaaaccat agtgaacaac
    39241 gttcccttcg gttgtcttga tatagctctg cctttcccaa acgtcataaa ggacatggtc
    39301 acgacgacat cttagttcca aggtgtcctc tggtaaccaa aagaatggca agatgatgta
    39361 gttctcctcg ctatgtcgtg gtgggaaaac caaaacaaag gctgtaatgt cagatgtgct
    39421 tgataagtca agccctgcgt aacagtcacg acctttaaga gcttcataat ctatcggggc
    39481 attacctttg gcatagacat gttcaggaat ccaagccacg ctagaactcg tccacatgtt
    39541 gagtcggagt tgcttaaaga cattctcctc tgctgggtta tcaagagcct gttgataggc
    39601 ttcacgaaca cggtcaatcc caatggtgtg cccaagggac ggattggctt ttagccagtt
    39661 ggcttcgtca ttccaatcat cttcatcaga aaggccataa accactggat aaaaggacgt
    39721 gtcctgcttt cgacctttaa ggatatcgag agccttggtg tggagttcat aacagatgga
    39781 gtttttatct gtcccagctg ttgtgatgat gaaaaagaga ggttgttccc tggcatcacc
    39841 agaacctttg gtcaagacat catagagatg acgattgggt tgggcatgaa tttcatcaaa
    39901 gacaagacct gacacgttaa gaccgtgttt tgtccctgtc tcagctgaaa ggacttggta
    39961 aaatccagca ttggaataat tcactatcct ctttgtcgcc cccataatct tggagcgttt
    40021 ctcaagcggt cggctcatga gaaccatttg tttggcaacg tcaaatacaa tggaagcttg
    40081 gttgcggtca caagccgctc catacacttc cgcactggct tcattgtcag catacaaaag
    40141 ataaagggca atagctgcgg caagttcaga cttaccgttt ttcttcggaa tctcgatgta
    40201 ggctgtcaga aactgacgat taccatcttc cttaacaatc ccaaagaggt cacgaacaat
    40261 ctgttcctgc cacggcaaca aatcaaactt cttccctgcc cacttcccct tggtgtgggc
    40321 aaggttgttg ataaaggtaa ctgccctatc tgcctttgcc ttgtcgtagt gggaagtcgg
    40381 aagcataaaa ggactcggtt catagtgata agtcatagaa tacctcctaa caaatcctcc
    40441 atctcatcac cagtaccaac ctccgcatcc atagtcgcca agcgattacg agctgatggt
    40501 gtcaaaccaa actgctcaca gaacttgagc atgattttca ggttggtttg actgatagat
    40561 acctgtggca cttgttggag ataaccattt ggtgttttga tgatggaccc atgtttggag
    40621 agaaactctt cggcttcctt ccaacgtgca taggcttggc aatagcctgc gaaggctgtc
    40681 atatccatct ccgttaatat ccccatttgt tcgaggattt ttcccatccg tttccattcc
    40741 ttcttggcat catcttccag ccactgtggg caacgtgggg ctttctgttt gggcttaacc
    40801 tcgttaatcg gtagaggtcg tttccctgag tttccttcaa gtattttcaa attggtaggc
    40861 tttggttttc gccctctaac tgccacggtc tcacctcctt tttggcacaa aaaaaggctt
    40921 caaacagaaa ccttttagtt cttaacaaca ctttcaatct cctcacgaga taacttaaca
    40981 ctcggttcta agttatggta atgtcgttca tcttcaaaca catcaatctc aactctagcg
    41041 gtcggactag tcattaagct aataacctca tcatgcgata agggaacggc tttctcacca
    41101 ctatcaaaaa cgtatccatt gtacccctgt ctcagatagt tgtagagact ttgaataaag
    41161 ccgtcacggt caggctcaac cttgaccaca ctatcaatac cgtacaatat gtttaagctc
    41221 tgtccattat cccaagagac aaggagagaa ccaatgtcat ctacatcaag caccgtgcca
    41281 agcatcccca ctggaactgg gcgagggtcg tccatctgga ctaacctcac ccttgtccct
    41341 tctgggtagg ttgacttgat tcggtttaga atcttgtcgt tcatcttatg ctcctaatgt
    41401 ttcaaatgcc catttgactg cgtgaccttg gtcttgaaag gtttcttctg aggttttctc
    41461 aaggttaagg tctgcctcca tgcgattggt gtactcgcta aggctctcgt atttgtaaat
    41521 ctcagccaca atgcctttgt aagttggtgt ggcaacaatc acccaatcct tgtaaggaag
    41581 gatgcttgcg gttgctggat aatggctgta aagtttttca agtgttgtgg ttgtcatggt
    41641 tttgttctcc tcttcttttg ttggttacat attacctcta aagaggactt atatccagtc
    41701 atttctgctt attatttgca gatttattgg cttctaaaat agctttccca atggcataaa
    41761 ccacggttac tgtcacgcca ttgccagcct gtttgtagag ctgggcatca gagttaacag
    41821 cctgagcctt gtcaaacaag tcatctgaaa acccttggag tctgaaacac tctcgtgggg
    41881 tcaaacgtct gattttcacc actcgaccct tccaaaccac ggcacccatc tgaccaccac
    41941 aagagaggtt gtgtgcgatg cccttcccta ctctggctcg tctcgtctgt gagcctggat
    42001 aggaaaggtc aacagagtcg ccaacctctg cgacttggta gtcctgcttt gtcccatttc
    42061 tgaccttgat gccttcaagg acaccgtgac ggtcttgaga agtcagagta aacataggct
    42121 catcctgttc ctttagccgt ctaccatttt ggcgtttagt gattctatct ggggtcagaa
    42181 tgggctgaac ctcaagaaca ccagagttca ttgctgttct ttttgtagct cctgccgtat
    42241 aacgagcggt tatacaccgt gcttcttcag ttagttttgg ctcggtcaaa gactggtcaa
    42301 tcagataaag acctgtctta gctcctagtc ctcccccctc accaacaagg gttgtggcaa
    42361 tgccacttgg gtcgtagact cgatagcttt gcatgccacc tacaagttgc ttaagatagc
    42421 taccgccttc tccgctgaga ggtagtactt gtcgtcgacc tcgacttcta agatgtccga
    42481 gagtgtagat acgttctcgg ttttggggaa ctccgtagtc ttttgagttg aacacttgcc
    42541 attcaaggtc gtaccctgct tcatccaaga tagagagata gtcgagataa tctcgtcccc
    42601 cgccacttga tagaagtccc ttaacatttt caaggagaac ccattcgggt ttatcttctt
    42661 ccttttggct ttggatgagg tcaacaaatg taaaaaagag tccacttcgc tcaccgtata
    42721 ggccggctcg ctttcctgcg atagacacat tttgacaagg gcttcccgca gtccataaat
    42781 ctgcttttgg aagtcgtgtg gggtcaatgc ttgtgatgtc gtcatgaaac cattctcctt
    42841 ctgtatcgta cattgcttca taggatttcc gtgcaaactt atccttctca caatacccaa
    42901 gacaggtcat acctgccaat tccaacccac gacgaaagcc acccactcct gcaaagaaat
    42961 caagaaaggt tagagtcata ggtcatcctc catatgtgct ttagcttcgt cataagataa
    43021 ggtctgacca tttcgaagga ctgtcacatc ggaattagca gtcgactcca tgtagcgttt
    43081 gacaatgaca tccacaaact tttcatcaag ctctatgccg taacagattc gaccagtttg
    43141 gtcagctgcc atgagggttg agcctgaacc aagaaatggg tcaagaacta acgttcctcg
    43201 catggatgag ttttggatag gatatgccat gagatgaatc ggcttcatgg ttggatggtc
    43261 tttactagac ttaggtcggt catattccca gatagttgtt tgcttacggt cactgaacca
    43321 ctggtgtttt cccttttgtt tccaaccaaa gagacagggt tcgtgttgcc attggtaggg
    43381 actgcgtccg agaaccagtg agttcttctt ccaaatacaa caaccgctca gatagaaacc
    43441 agcatccttg aaagccttac ggaagttaag cccttccgtg tccgcatgga aaacatagat
    43501 tgaagcatca gcttccatgt ggctttcaac ctgagtaaac atgtcataga ggaattgata
    43561 gaagtccccg tccgacatat tatcgttgag gatttttcca gccgtctctt ccacgttcac
    43621 gttataaggc gggtccgtca caacaagatt ggctttttta tcaccaagta gctggtcata
    43681 agtttctgcc ttggttgaat caccacaaat cactcggtgt tttccaagtt gccagatgtc
    43741 tccacgtctt gcgacagttg gtttcttcag ctcctcttcc acgtcaaagt catcttcagt
    43801 caagtccttg tcgtggacgt tggagagaat gtcatcaatc tctggtggtt caaaaccagt
    43861 caagtcaagg ttgaagtcag actcttgcaa atccaaaagc aagtcagcta gaagctggtc
    43921 atcccattga ccagtgattt tattgagggc aatattcagt gccttttcgt cttccttgga
    43981 aagagaaaca atgacgcact tggccgtttc atacttgagg tctttcaata ccgtcaagcg
    44041 ttggtgacca ccaatgactg tcaaatcctc gttgacaatg attgggtcaa cgtagccaaa
    44101 cttgagtagg ctttgtttaa tcttttcgta ctccttatca ccctttttca gtttctttcg
    44161 agggttataa gaagctggtt ttaagtcact caggggaagt tccttaatct ccatcttggg
    44221 ttgcgttgtc attagatact ccttttctaa atcggtattc cacgtaacaa gggtgtccgc
    44281 agaacttcct ttttggattg gcataagata aaaaaaactc accacagttt tggcaagtca
    44341 attcatcgta tgcagttttg gttttatcgt gttggctttg atggtttcgc caccaaatcg
    44401 ctcggcagct atttgagcag aactttttag gtctgccttg gacagcatgg tgtaattttt
    44461 tcatgcagtt cttacaataa ggacgttctc cttcatcaag ctgatacttg acgaactgac
    44521 ccactccttt taactctggg tgtctgcggc aatattgctt caccgaacca agagacagat
    44581 ttaacatttg agcaatggca ccatagccaa atccatctcg cctaagcttc caaatgcctc
    44641 gccgctgatt atcgttcatt tgttttcctc caagaactaa aaatggttat tttctctatt
    44701 tttcacttag ttaccccttc aaaaacaggc taaaacagac actagcaaac gatacttacc
    44761 ctgctagaaa gattgacacc ataatggttc aatgcacttt ttagctgttt tcggtatgcc
    44821 cctaacaaat tttgcgaaaa tgcacgtttg agggggcgtc ggtctttgtg ggacaagggt
    44881 ttagagattt atacccccct accccaaatg atggaaaaag agatactttt gtaacgaaac
    44941 tctaaattta aaaccgataa gtatattcca catatcggtc agtcgtctta gtctttcggt
    45001 catgacaaga tttacagaga gcttgccagt tggattggtt ccaaaagagg tcttggtctc
    45061 ctcggtgggg agtgacgtgg tcaaccaccg ttgccttggt tagtcgtcct ttcctttgac
    45121 agtaaacaca aagaggatgg agctttaagt aacgaagccg tgccttgttc cagcgagagt
    45181 tgtaaccttt ggctttggtt gacttggcat caagggtatg gttagctttg tggtcatcgc
    45241 agtacttgtt tccataggtc acaaggttag gacaaccatg ctgcttgcaa ggggtgcttg
    45301 gtcggcgtgg catcttactg ctcccaagga agataaggct tggtgaaatg cccaaggcaa
    45361 gtggtcttgg tgtagtccac atccaagagg ttcagctcct tgataatccc ttgtggggta
    45421 agatcatagc gttcacgaac cactcctact agttgttcaa gaagataatc acttgtccca
    45481 aaggtgttca cataaacacc tacaggctcc gcaaccccaa tagcataagc taactggact
    45541 tcacagcgtt tggcataccc ttcacggaca aagtcctttg caatcttccg tgccatgtag
    45601 gcggctgaac ggtcgacctt agagggatcc ttaccagaaa aagctccacc accgtggtgg
    45661 gcaaaaccac cgtaggtatc tgccacaatc ttacgaccag tcacaccagc atctgcgtaa
    45721 gagccaccga gaacaaagca tcctgttggg ttaaccaaaa ccctaaagtc aaggttctga
    45781 cggtagcgtt gagcaaccga catcatggtt tgagtaacaa tgcgtttcac ggatgcaagg
    45841 tcaacctcct catcatgttg gatagagaca aggaaggtct ctatacgttt gttttcgtag
    45901 tcataagtga cttgagcctt ggcatcttta cccaaggcag gatgaccaag gttcatcagc
    45961 ttctcaagca cccgagtcgc aagtacataa ggtagcggca agaactcagg tgtttcatcg
    46021 gtcgcataac caaacataat gccttgatca ccagcacctc ccttgttcac accttgagca
    46081 atgtctggac tttgaacacc gagaaggttc gtcaccatga tatcctccat gccgtaaggt
    46141 tcaagaactt ttttgacaat gccttcgagg ttgaaaaagt gcttggttga gacttctcct
    46201 gccacaacta cttgattatc cttaatcagc gtctcaactg ccacacgact attcttatca
    46261 tgcttgagac attctgtcac aacagcatct gagatttggt cacagagttt gtctgggtgt
    46321 ccgcttgaaa cttgttcact ggtataaatc atgttttcct ccatgcaaaa agcccaaccc
    46381 ttttgggcta ggctttggtt tattttactg attgttgacc tgcttcgtag gctctctcga
    46441 gtgccctttt aattccccaa accgaaacat cgtagaaatc aaggttgtca ctcatgcgag
    46501 tttctaaagt ttcaaccaaa agttcttctt gagcaatcgc tgttaaaagg gtgttgagtt
    46561 tttcttgttg gcattttgtc attttgacga cctcctcttg tttttgtagc gttatattac
    46621 cgtacaagcg aaggtatatc cagtcattac tgggagattt ttatcttttt tgacacttac
    46681 aattctacca caatttttta caaagggagg tcaatgttag gtcacattta gtgcaagggg
    46741 aggttactgt taggtcaggg gagtgcaagg gaaggttatt tttctaaaga ataacccatc
    46801 ttcagataaa ctcccctaac atgatccaat accttacgac gccaatttct aaccgtacta
    46861 cggctaatgt gaaactccct catcaaacta tcccaattac actccggctt taacatttct
    46921 tgtgcaaact cagctaggtc atccttaaga aatcgaatag ctatctcaaa attatcaagg
    46981 tcatttgcca gtcgtatata tcgttgagtc aaatctgcta agagctcttc attttcctga
    47041 atcatcttgt cacgaaaact tagggctatc acctctgaac gtagatttgt cggagtagag
    47101 gtgactttag gttcatccga ccgttcgaag acaagtgacc caatcacttc attctcagtc
    47161 acaggcttaa aattctccaa gcgatacttc aacatttcca agtccccctt gagttcatta
    47221 taatgcgtta ggatatattc cgctttatcc atccgttcct cctacttgtg ctttcacagc
    47281 ttcaatcagc cgtgattgtt gggcatcttt gttttccagt gctttgagga tttcctcgtc
    47341 aatagtgcct tccgttacaa tgtgctgtat gaccacagtc tctgcttgtt gcccttgtcg
    47401 ccatagacgg gcgttagttt gttggtagag ttccaaagac caagtgagac cgaaccaaac
    47461 caaatgatgg ccgccctttt gtaagttaag tccatgacca gcacttgcag gatgtagaag
    47521 tccaactctg atattaccct tattccactc acggatgtcc tgctcagttt taaggactgt
    47581 gccctgaacc ttgagctttg ccaagcgttc ctcaatacgc tgaaggtcat gcttgaacca
    47641 ataagcaaca agaactggct caccgtttgc tgcttcgata atatcttcca gagtatcgag
    47701 cttctggtca tgtaggctca ctacttgatg gtcgtcagag tagacagcac cattggccat
    47761 ctgcaccagc ttgtttgaca gactagccgc attggcagct gtcacctcac catcttccaa
    47821 atccgaaagc acatagtctt tcttgaatcg cttatagttg tccgtttctt tgtcagtgag
    47881 atgaaccacc ttcttggtcg aaatcaactc aggcatatcc aaataatcca tggctttcat
    47941 ggaaatggta atgtcgtcaa tcttgtcata gatttggcac tccgcatagt ccattgggat
    48001 atactcgtag acaatattgc cgttcctacg tccctcgtca aagtatcgac tgcggtattc
    48061 tccaatgaaa cgtcccaaac gctcaccact atcaatgacc ttaaactccg caaacaaatc
    48121 cataagccca tttgaactag gtgttcctgt caatcccacg acacgcttca tgtaggggcg
    48181 catagccatg aaggccttga aacgtttgga ctgccatgac ttgaaagacg agagctcatc
    48241 aataactacc atgtcccact tgaagtaggg actacattgt tgcaccaacc aagggagaat
    48301 ttcacggttg acgatgtaga tatccgcatc ttgctccagg gcagctcttc tctgttttgg
    48361 tgttccaaca atcttagcat aacggaggtg tctcagctcc tcccactgct ctatttcatc
    48421 actccatacg gtgtttgcaa cacgaagagg tgcgataacc agtacctttg agacttcgta
    48481 gcgatcaaac atcagctcgt tgatagctga taaggtcgta gcagtttttc ccatccccat
    48541 gtctaagatg accgccgcat aaggggttct tatgatgaag tccttggtga cttcttgata
    48601 gtcatgtaaa ctcaatttca tctagcactt ctccaatctt ctcaacactg tccagcacat
    48661 gaactttgaa gcctaatcgc tcaaatagtc tgtgccttgt cacttgtagt aaacgtggct
    48721 ttccaccagg agctttcacc tccaccatgc caaacttacc cttgggtaaa aacaccaacc
    48781 tatctggtac accgccaaaa gaaggtgaca cccacttagg acaaattcca ccacgctttt
    48841 tcacttcact tactaacttc tgttcaacaa ctttttctcg cataacaaat cctttcgtca
    48901 aattgacgtg tggaggtata gtacagtcat ttctaaaact ccccttatag gcttttttat
    48961 agtaagtttt acttaaagga tagttttaga aaagaccata atagacctac acaaaatcaa
    49021 atcatgttag tcgtgctagt ccttttgact aatctgttct agtgagtaga actcataaat
    49081 tcttgtcatc aggtcaatga ctagcacatc taaaatccct ctccatctcc cttgtggaag
    49141 taggacatgg aaaatgatgg tcactagtct aggaaatcat agtcatcatc aaccaatttc
    49201 aaacctagaa taaggtttcc tttattggta cgtttgcgtc taaatcctgt ctgatccagg
    49261 gcagagtaaa aatctgtcgt gctgcgcgtg tactccatat ttttggtaca gtaggcacgg
    49321 tactgactgt agagttctcc agatttttct gtaaactgat caccaacttc gcaacagtca
    49381 ctaaggaagt gccctaacca atcattggct tctctgtagg ctttgacgga atttgaaaca
    49441 gcagatggca cacttgtctt gaagtttgcc ttgatggctt tttctgcccc ttcaataatc
    49501 caagacataa tggctggtgc tgcatggtcg tacaaataat ccgcaaagtt cttgatatca
    49561 gagcgaccag tgattttggc gttaaaagga ataaccacca aacgtcgcca agtcccatca
    49621 tcgttcgctc ccactttagg cagatggttg gtataaagaa ccagcgtatg tgatggcaca
    49681 aagtggaagg gatccttgta ctttttctct gcttggattt catctgtaga ggtaatctgc
    49741 ttaacaacgg ctgtattaag tcgcattcct tcagccatct cagaagcaat cacgagacgc
    49801 tttcctttaa gctccgcaag ttcaggactg acgtttcgct tgttagacat ggttaaggca
    49861 tccgccgata atttcccaga atagcttcct agtacacgag caatggtgtt ccaaaaggta
    49921 gacttaccgt ttgcaccgcc accataggca ataatcatgt gttcctgata aaccttacca
    49981 atcgctgcca tcccaatgat ttcttgaaca tagtcaatca actcttggtc gttacagaaa
    50041 aaggtagcca aggtttcccg ccacaagtcc tggcctttgt cactaggaga gacagctgtc
    50101 attttagtaa tgtagtcttc aggattatgt tcttgctgtc cttttgttcc ttgtcgcaaa
    50161 tcataggtag cctcaggagt gttgagcaac atgtcatcac tatccaactc tgataattca
    50221 accgtaagca taggctttgc ggtattatag acagccatta aattcttata gtcacggtgt
    50281 ttcatgacaa atttatggaa ctcttttgcc gcaagatagg ctttaagata tttcaactgt
    50341 agagaggtct caaccgcatt ttctagacgt ttacctcctg ccttgatggt catttcatca
    50401 atacctgaag attgaagctg tttctctgtt gtttctaaaa aggtatttgc ttccgcaagt
    50461 tggtcatcgg taaagtgtac aactgcccct agtgccagct gcttattctc acgccagtgt
    50521 gttccatcgt agaaaagata gtctgtcgca ttggtgtagg ctagtttatc tgcatattct
    50581 ctagcaagaa ccccagcttc accaacatct gaatagtcat caggttttag tgattccctt
    50641 tgaaactctt ctggtgactt ataatctttg gagtttttga tggttttgtt atagaagcga
    50701 acagcactgc cccaaatagt gtcaagctct gctttctcaa gaggaggaac acatttgagt
    50761 gcctgttcat caaaaccgtc tcgtgcttct ttcgttacac ctaaacgctt aagaattttc
    50821 gccgcaaaaa ctgacatagt tgagttgcga ctaccttcag tgattggacc tgttgttggc
    50881 tgatagaagt ctgcatcaaa gtcctcttca tcatcttgag caaccatgtc atccagtaaa
    50941 tcctcatcaa tggtcaacca agaatcatgc cacaagactt gtgcgttagg atttccaaag
    51001 aagaaacgag ccgcatcttt ggcattagtg tcaaagatgc catagcgatt tacaagttct
    51061 tctttcagta aggcataggt gtccttatct gtgatttcat tgatttggaa gtagatatgg
    51121 aatttgggtc tggcagcctt atcccctttt tgaaccaaat ggttacgact ggtcactaaa
    51181 gcaaagttgt aatccgcaaa gatatcttta agtagctctt ccgttaccca ttcatcagag
    51241 ttgtccgtat ggtcattatc aatatccatg accaatacat ctgatttgat gaagttggca
    51301 ttggaacgag tattattggt aaacaagcca gccacatggt caaagagtgc gaccttttgt
    51361 agagttgttt tgtctgagat cattacttga tgaggataga ccgttgtcgt ttgaatacct
    51421 gtctgtcctg aatgagataa ggtaaattgc ataaatcata gcctccattt tggaaaaata
    51481 atctgtgaag gtttcacttc ctacctacta ggggaagaat gaaggcgttt agtccaaaaa
    51541 gtttcaaaaa aattttttct tctataaata actattgagc gaccttccat cgatcaattt
    51601 ttttatattt ttttaaatct gtttagacta aaacgcaaaa aagtccccct agtaggtgta
    51661 agggcgagat aacccaaaca aacctaaagg aggactttct atggaaaaca ccataaaccg
    51721 tcaagaacaa gaagacttga cagacactct catcgccatc agcgttatcg ctaggctctt
    51781 agctcgtaag ttacaaaagg aggaatcgaa atgagtcaac ataaacaact acttgaacta
    51841 attacagaga tggaaggcac agctaaatac tacctacgct tggtagatga gttcaagaaa
    51901 cttctctcta ctgaggaaga gacagacaca atttctgaag agccaaaacc taaaccacaa
    51961 aaggaactca agttggaaga tgttcgtgcc gtccttgcga ccaaagccaa agatggctac
    52021 aagaatgagg tgcgtgctct cctcaataaa tatggagctg aatcattatc tgctttagcc
    52081 actgagcact acgcagctgt tcttgaagaa gctggaggaa ttggcaatga ctaatcacgc
    52141 agtactctct gcttctgctt ctcatcgctg gctcaactgc ccaccttctg tacgacttac
    52201 cgaagatatg ccagatgtga cctcagagtt tgcccttgaa ggaacagacg ctcacgagct
    52261 atgcgcttat cttgttgaga aggcattggg taggaaggtg cgtgatccaa ctaaggactt
    52321 atccttctac aatgaagaaa tgcaaacctg tgccgaggaa tatcgcaact acgtcatgga
    52381 gcaagttgag aaagctaaag gctattctcg tgacccaaca gttctggttg agcaacgact
    52441 ggacttctct aaatgggtcc ctgaaggctt tggaacaggc gactgtctga ttgtggctga
    52501 tggactgctt caagtgatcg actacaaaca cggacttggc gttttagttg atgcagacca
    52561 caacccacag atgatgtgct acgctctagg tgctcttgag atgtttgatg acctctatga
    52621 ttttgataag gtcaccatga ccatctttca accgcgaaaa aacaacatct ctacctttga
    52681 aattgataag actgaactac ttgagtgggc agaaacagaa ctctcaccca aagctgaact
    52741 ggcctttaaa ggagagggag agatgacctc tggtaaacac tgccagttct gcaagctcaa
    52801 gaatgtctgt cgcaaacgtg cagaggataa cttggcactc gccaagatgg aatttgcaga
    52861 ccctgctact ctagactatg aggatattgc agagattttg cctaaactgg acttgctggt
    52921 ttcgtgggca aatgatgtca aagcctatgc tttgaaagaa gcaactgaag gacacaatat
    52981 tccaggctac aaacttgtag aaggacgttc ggttcgtaag ttttcagacg aagctgctgt
    53041 cagtcaagct gtgatggagg ctggctttga cccttacgaa aagaaactcc tgaccatcac
    53101 tgccatgagc aaactccttg gcaagaaaac cttcaatgac ctacttggtg gtctgattgt
    53161 caaaccaagt ggtaaaccaa cactcgttcc tcttgatgat agtcgtcaag agatgaacct
    53221 agctaaaaat gaattcaaag aggtataaaa gaatgtcaac taaagtacaa acaacaaaag
    53281 tgattactgg taaaaacaca cgcttcagct acttgaatgc caatgaacca aaatccatta
    53341 acggaagcac accgaagtac agcgtctccc tcatcattcc aaaagatgat attgagactg
    53401 tcaacaaaat caaagcagcc attgagcttg cctacaagga aggtgaggca aaactcaaag
    53461 gcaatgggaa aacagtacca gcactttcta tccttaaaac accactccgt gatggtgacc
    53521 tcgagcgtcc tgatgatgac gcttatcgca atgcctactt cgtcaatgcc aattcaccac
    53581 acaagccagg ggtcgttgac gggaatcgtc aagagattat cgacacctca gaactctact
    53641 ccggtatcta cggacgtgcc tctatctcct tctatgcctt taactctaat ggcaacaagg
    53701 gtatcgcctg cggtttgaat aacttgcaaa aacttcgtga cggagaacca ctcggtggac
    53761 gtactcgtgc agaagatgac tttgcgactg atgacgatga tgatttcttg aactaataga
    53821 ggaggatatt acttatggac atttacacaa ttttactttg cataattctt ggtatttacg
    53881 tctttcttgg actctatctc aactacatga ccatccgtga cgatattcgt cgtgagaaag
    53941 agcgaaaagc tgaaaagaaa cgtcaagaca acaacacaac tccgctacat cgtagccgtt
    54001 aatacttctg gtggcagcac tcctgccacc tttttatgaa aggacaaact atgccaatta
    54061 aagaactatc gattgactta gagacctact gcgaagtgga tttgagaaag tctggtgtct
    54121 atcgctacgc tgaagatgat tcctttgaaa ttcttcttct agctgtctca gttgataacg
    54181 aaccagtaac cgtttatgac ttaacaaagg aagaacttcc tgatgaaatc ctacaagcat
    54241 tggtagatga caccatcatc aagtgggcat ttaatgccag ctttgaacgc atctgtctgt
    54301 ctaactggct caagaaacat cacccagaat tactatccga aggtttccta tctccaaact
    54361 catggcgatg cagtatggtt tggtcagcat atcttggcct accactctca cttgaaggag
    54421 tcggaactgt cctcaagcta aaagaacaga aacttaaaga aggtggagac ttaatccgct
    54481 acttctgtct cccctgtaaa ccgaccaagg tcaatggtgg acgaacacga aactttccca
    54541 gtcactcacc tgataaatgg tctgctttta tcgattacaa ccagcgtgat gttgaggtgg
    54601 aattggccat caaagagaaa ctccgtaact accctgttcc agtctttgtt tgggaggaat
    54661 accatcaaga ccaaatcatc aacgaccgtg ggattggtat tgatgtggac tttgtcaatg
    54721 cagctattgc cattgacggg gaaagtaaga ctaaaatcca agaagaattg agaaaactaa
    54781 ctagacttga taatcccaac tccgttcttc agatgattgg ttggttaaga gaacacggag
    54841 tgaccactga ttccctcgat aagaaatctg taaaaaaact cctcaaaact gtcgatgaga
    54901 aaactgcaca agttctcaaa cttcggcagc aagcagccaa atccagcgtc tccaaatacc
    54961 aagcaatgat gaactgtgtc tgtaaagatg gacgtgctag aggaatgttc caattctatg
    55021 gagcaaaccg tactgggcgc tgggctggtc gtttggttca gttgcaaaac cttccacaga
    55081 accatcttcc tgaccttgag gaagctagaa aacttttcaa aactggtgac ctagaagcta
    55141 ctgagctact ttataacacg cagtatactt tatctcaact tatccgaact gcatttgtcc
    55201 caagcgatag aaaaaaattc atcgtctgtg atttctctgc catcgaggcg cgtgttctgt
    55261 ctcatcttgc aggagagaca tggcgaagca gggtctttga aaaaggaaaa gacatctact
    55321 gcatgtctgc gtcacagatg ttcggagtac ccgttgagaa acatgggcaa aacgctgacc
    55381 tgcgtcaaaa agggaagatt gcggagttgg cttgtggata tggcggagca gttggtgctc
    55441 ttaaagcaat gggcgccatt gatatgggac ttgaggaaca agaactccaa ccactagtgg
    55501 attcttggcg acaagccaat cccagcatcg tccttttttg gtgggatgtg gatagggctg
    55561 tgaaaactgc cgtcaaagaa caaatccaaa ccgaaactca cggcattcag ttcgaagtca
    55621 gaaatgggat gttatttatc actctccctt ctggtcgaaa acttgcctac gtcaaaccca
    55681 agatgggtga gaaccaattt ggtggagagt ctgttaccta tgaaggtaca ggaactgcta
    55741 aacattggga gcgacttgaa agctatggac caaagtttgt cgagaacatt gtccaggcca
    55801 ttagccgtga tattcttgcc tactcgatga gacaacttag tgagttcaag attgtcggtc
    55861 atgtccatga tgaagtcatc attgagtgtg atcaagacca agaccttgaa gaaatttcaa
    55921 ccttgatggg gattgcacca gactggatgt ctgacattaa cctccgagct gatggttatg
    55981 aatgttcctt ttatcagaag gactagacac acaaaaaaac ggttcactac taattcaggt
    56041 agtgaaccat tttatatgtg ataaaaacta tttccttaat tgattgatga aatcttcctt
    56101 ggaaattgct atacccaatt catcatgaaa gttgcgtaga gtgctaaatc ggtatgggtt
    56161 caatgtccta aaagtgacag tttctccctt ttcaatcttt gaacaaatct caatatactt
    56221 atttacgtaa ttcttaactc gtgagaaaat acgatttgaa tttcttagat aaaaaatctt
    56281 ctcatttgga tttttaaagt gatatattct tgaattgtca atatattttt cctcgataat
    56341 cgtcaactta ctgaaatcta aagccttcag acaaattctt ccatcgtgac gtctataggt
    56401 tgtgtaaggg aataaatgac ttgcattcct aagatgctca ggaagaccgg agcgaagcgg
    56461 aattgcaatc agttgattat tgatatcaag aaccataaca ccatgtccac gcgcattatc
    56521 ttcttgaaac tcatccaaat attggtaagt atcgaaatag tcttgagtta ttagatgaaa
    56581 atacagacta ttcttattga tttttgacat aaactaaacc tctatcatac gaaaaaaggg
    56641 tctttctttt tagagtcgcg acccttacga ctacatcaac tttagagtat tttcttttta
    56701 gagtcggtat tactctccga ctacttctac ttaaattata ccaaaaacat cgctagtgtc
    56761 aaatataaac actatttcgg attgaatttc ttcaataaag ccgcaaccaa agcacgagcc
    56821 tttggcaagc gatagaaacc tgtagaacgt ttaaccccaa tcctagcaaa tacatcttca
    56881 atactctcat caaactcttt agcgagtaag cccaaagtga tatcatacaa atccttgtct
    56941 gcttgcctta ctttctcaag taaatcttgc agcatttctg caaacataaa ttcttccaat
    57001 tcttcagtag aactgtaaag agtagattca tccatatcaa tccaggcttc atatgagata
    57061 atctcttcaa tattctttga cttacgtgta cggaactcat tcaaaaagtt ttcaacggca
    57121 tctttataga tacgttccgt tttagcatat tcttcaggaa agactgggat aaaagcaacc
    57181 agtacataaa cattcagctg tttagctggc ctccaagacc gaagggtctc tcttttgaga
    57241 cgcatatcct caataacttg ttggttctca accaacatag gagctagcat ctgcccctct
    57301 aaaagtccga cttctaggga gcattcttgt ttttgacatt cgttataaat attgtccttg
    57361 tttgtcatgt tttccacttt ctgtcttaag cagagaaacg tgacaagaca aagaactatt
    57421 aaaaagattt tctgaccaca tcagcaattc ctctgctaat tcatggtcaa ctgactttac
    57481 aagttgaact gcacacctaa tttgtaccct taaagtcacg gtaaatcagt ttcactcatt
    57541 tacaaagtat catcattagg caacaaccat ctcaaattac ctattagcgc tattcgataa
    57601 tcgagatttt ttacattcgc aaaacttttt atgcgttcgc aaatatattt ttgaacatcg
    57661 caatcctgtc atgatataat aaacttaatc attaacgatt actttgttcg actctattgt
    57721 attaaatgag attttgaatt cggggacaga ttagtacaat acggtacagc ttggtacaaa
    57781 aatgaaaagg agataatcac ttggattttc ccacttattt tagaatactg aaaaaatatc
    57841 ttggagatgg cgcgactatc ccagagtttt ttcgagaact cattgaaatg attactgatg
    57901 atgacgccga agatatcatt agtgcttctg gtattacatc tgacaaagca gatagtacgc
    57961 ttatctctta tacaaagccc aaaagaggct tcaccaagaa gatggcaaat caattgctct
    58021 accgggtcaa ttcagctaat atgacggagt ctattgaaag taggccagat gaaactattc
    58081 agttactagc taatgaattt aatacttatt atcctgatat tactgctgaa aatgcctcgc
    58141 aaagaattcc agaaattttc gttgacttca ttagagagaa agctgggatg ggaatatcaa
    58201 ccacagctca aaaagctaca tttattaacc agtcgaatca actcaaaaaa caatatggac
    58261 aattcttact gacggaagca aataactgtt gtgcattccc aggctgtgat agaccactca
    58321 ttttaacaag aggtggttta gcatcggaaa actatgaggt ttcaaccatt gaaaaagata
    58381 aggacgctga accactgaat ctgatagccc tctgcccaga ttgtttttta acctatcaag
    58441 cagaaagtcg caaaaaaatt gtaacagctc taaaaaatgt taaaaagatt ctagtctctg
    58501 cacataacag ccaacaatcg atttctgata tgaagttaga tagtggaatt gtctcagttt
    58561 tgactagtct caataaacta aaatttgatg aatatgatat ctcctatgac ccgaagcaat
    58621 tgactgataa gatatcacct gagaacaatc gtacgcttta tcaattggtt aagaaccaag
    58681 ttattgataa ctacttaacc attcaaaaaa tcattataaa tttagataaa caaggaagaa
    58741 tcgattacga ggatatccaa tatcaaatgc gatcgatgta caaaaagtta aaggctgcca
    58801 agcatggtaa tcttgagatt ttcaatacta tttcggaaaa actccataaa gctacactac
    58861 aggacatcta cttctgccag atgattgtct cttactttat ccaaaaatgc gaggtacttg
    58921 aataatgcaa ttaccaaata aactttattc ctatcaaaaa agcaccctag cctatttacc
    58981 tagagtgcta catgaaatta aaatggggaa ctcaaatgtg aaagatatct ttcatactat
    59041 ctcagaagag ttggaggatc cgacagattt cttatccatc atggattgct tatatacatt
    59101 aaacgccatt gaaatgacca atgaaggaga ggtaacatta tgcttataga aatgtggtcg
    59161 ccagtcttta aaaaggatgg acaaactcgg gaaccgattc aatttcaccc tggattaaac
    59221 gttatcatgg gtatggactt agctaataat tctatcggca aatcctcttc tttattagca
    59281 attgacttca tattcggagg taatagctat ctaaaatcaa ttgcggttaa gaaattaggt
    59341 gatcatccta tctacttctg ctttcaattt gagaaaaagt tttatttttc aagagataca
    59401 gccaatcctg atatcattat agtgtgtgac aagaactact ctccaactgg tgaaataatg
    59461 gaccttggaa cgtttcttaa caaacttaag aaacgatatc atttggaatc ctccgattta
    59521 tcattccgat tagctatgag tggtttcttt agaattgcag ggaaaaataa tcaaaatacc
    59581 gatttcccgt tacaagttta tgcaagccaa aaatcttccg aatccattac aacccttatc
    59641 caactattta atttgtatga taacattgca cgatacaaag aaagacttaa ggataagagt
    59701 gaccagttaa caacgtttcg caatgctcga aaatatgaat ttatctccaa cttagttggt
    59761 ggtaaaaagc aattcgagct caacgtctca gaaattaaaa gattggaata tggcttatca
    59821 caccttcaag atacttatca agacaacatt tgttcggatg acattgagaa acatcagcaa
    59881 aaactccagc ttagaaatac taagttggag ctagaaagta ttctccgtga taaacaaaga
    59941 cgtcttaagc tattagatat cagtatcgaa tttggtttat atcctactga atctgactta
    60001 acagagcttc agcaatactt ccctgatact aatctcaaga aattatatga agttgagacc
    60061 taccacaaaa aattggcaac tattttagat tccgaattct caaccgaacg tgaatcccta
    60121 gtaggtgaga ttgacgaatt agagagtcaa ttagtaactc taaaccaaga acttcaagaa
    60181 ctaggaagca tcccaaatct ttcaactgag ttcttagaaa actactctaa actaacagca
    60241 acgatcaatg cactcaaaga gcaaaacgaa gcctatctga aagagtctgc tttatcaaaa
    60301 gaaaagtcag aagcagactc tgacttgaag cgtagcaccg aagatatact aatagagtta
    60361 gaaggaaaaa tcaatgctaa aatgcgagag ttcaataata ttctgtatcc agatattcgc
    60421 aaagcccctc aaatcaatct caaagctcat aacagctact ccttctacac cccagatgat
    60481 gatggtacag gaactaagtt caaagggatg attttatttg acctaaccat gctctatctc
    60541 actaacctgc cagcactggc acacgactca ttactcttga gtaatattag ctaccaagca
    60601 accgaagctc tgttgaagct atatgaccaa tcaaaatcac taaataagca ggtgttttta
    60661 gctttcgata aagcaagctc atactctcca gaagctaatc aactcttatt agaaaatact
    60721 gtattgcgac tctctagtaa cggaaatgaa ctctacggta tttcatggaa taaaggagaa
    60781 aactcagatg aagtttagct acaataaatt atggaaactt ctcattgata aagattggac
    60841 aaaaacaaag cttagacaag aagctgggat tagttcttcc acgatcgcaa aattaggaaa
    60901 aggggaaaat atcactactg atattttatt gaaaatttgt attacacttg attgtaagat
    60961 tgaagatata gtagaaatta tagataatga tgattaattg taagcgccca ataacaaggt
    61021 acctatcctc tcttccagta attctgctat actgtagaaa ataagaatac tgggggatat
    61081 ctgaatgacc aaacaaccta ttgttccttt ggaacaacta cccatttctc gaaaatttga
    61141 gaaaaagcga agaatgctct tatctttcgt tgtaaagtga gagagcttga tctctctttt
    61201 tactccccta tctcatctga acaagctatc gctatcttgg ataaggtatt gtcctatgac
    61261 cattaactta agtgatttag gccaggtcta tctggtttgt ggaaaaacag atatgagaca
    61321 gggcattgat tcgctcgctt atcttgttaa aagtcaattt gacctagatc ccttctcagg
    61381 tcaggtattc ctcttttgtg gggggcggaa ggatcgcttt aaagctcttt attgggatgg
    61441 tcaaggattc tggctcctct acaagcgttt tgaaaaagga aaactcactt ggccaaacaa
    61501 tgagtatgac gttaaggctc tgacttctga acaattaaac tggctaatga aaggattttt
    61561 catcactcct aacataaact gtacaaaaag tcgtgatttc tattgaaatc atggcttttc
    61621 tttaggtata atatagaaaa cattaggagg agatcattta tggaagaatt acttaaaatc
    61681 attaaacagc agtctaatga gattgctctc cttcgtgaac aagtcgttta tctgacacaa
    61741 aaaatttatg gcaaatcatc tgagaaaacg attcaaatta atgggcaact cagtcttttt
    61801 gaagaagaac aattaactga agaaaaagcg gacttaccca ggtgaacttg aaaccattac
    61861 ctatcagcgt aagaaggcta aaggaaaacg tcaaaccatt ctgaaccaat ttaaagccga
    61921 agaaagacat tatcacctaa cagaatgtgc ttgtccagat tgtcatgggg ctctcaaaga
    61981 aattggtgca accgttcagc gtcaagaact ggtctttatt ccagcacagc tgaagagagt
    62041 tgaccatatc cagcatgcct acaaatgtct ggcttgtagt gagaagactc tcagtgataa
    62101 aattatcaaa gctcccgtac ctaaagcacc actagcacat agtctagggt ctgcttcaat
    62161 cattgctcat acgattcatc agaagtttca tcttaaagta ccgaattatc gtcaggaaga
    62221 agattggact aaacttggtt tgcctataac acgcaaagaa atcgctaact ggcatattaa
    62281 ggcaagtcag tattattttg aaccacttta tgagctttta caccagaaat tactagaaca
    62341 gcccgtcctt catgcggatg aaaccagcta tcgcgtttta gaaagcgata gccagctaac
    62401 ttactattgg accttcttat ctggtaagga tgagaaagaa gtaatcactc tttatcatca
    62461 cgataagcgt cgtagtgggc tagttgccca agaatttcta ggtgattacg ctggttatgt
    62521 tcattgcgat atgcatgggg cttatcgtca attggagact gccaaggcag ataagagttc
    62581 tctggtgttc aaagggctgt gctattgtga taagatgttt gccttagagg agtcatgggc
    62641 tgatttatct ttacaagatc gcctgatgaa acgccaagat gagttagaac ctttgatggc
    62701 agagttcttt gactggtgtc gcaggcaggc tgttcttcca ggctctaagt tgggacgtgc
    62761 cattgactat agccttaagt atgaagaaac gtttagaact gtacttaaag atggtagtct
    62821 cgtcttatcc aataatatgg cggaacgtgc gatcaagtct ttggttatga gacgtaaaaa
    62881 ttggctcttt tcacaaagct ttgagggtgc taaatcaagt gctatcatta tgtctctatt
    62941 ggcaacagct aaacgacatc acttggatag cgaaaaatat ataggttacc tactagagaa
    63001 tcttccgaat gaggagatat tcgccaaaaa ggaagttcta gaggcttatt tgccatgggc
    63061 agagaaggta caacaaaatt gtagataaca aaatcttcca gagtcagtga actttggaag
    63121 attttaatat accttgttat tgggcgctta cgttatgact gctaaacaaa ttcaagaact
    63181 ccaaaaacga aatgctctta caaaatcaaa agtctatcta gattatacat ctagatagac
    63241 tttccaagca tgatgcttga acatattgaa tactctattt aggttggaga gagataatta
    63301 aacatagatc attcacaccg taaaacggag aaaagtttaa tgacatttca ggtcataggt
    63361 caaaagattt aatcctcttt tttcttagaa ccatacataa gagtgcttat taacaaaaca
    63421 attattccaa acaacgatag atgagtattg atctcgccag tagaaggaag tt
//
LOCUS       DBJYSH010000003        35356 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.003, whole genome shotgun sequence.
ACCESSION   DBJYSH010000003 DBJYSH010000000
VERSION     DBJYSH010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 35356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35356
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.003"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            complement(79..1218)
                     /gene="recA"
                     /locus_tag="ACUBV3_000140"
     CDS             complement(79..1218)
                     /gene="recA"
                     /locus_tag="ACUBV3_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001085741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HHU3846679.1"
                     /translation="MAKKTKKAEEITKKFGDERRKALDDALKNIEKDFGKGAVMRLGE
                     RAEQKVQVMSSGSLALDIALGAGGYPKGRIVEIYGPESSGKTTVALHAVAQAQKEGGI
                     AAFIDAEHALDPAYAAALGVNIDELLLSQPDSGEQGLEIAGKLIDSGAVDLVVVDSVA
                     ALVPRAEIDGDIGDSHVGLQARMMSQAMRKLSASINKTKTIAIFINQLREKVGVMFGN
                     PETTPGGRALKFYSSVRLDVRGNTQIKGTGEHKDHNVGKETKIKVVKNKVAPPFREAF
                     VEIMYGEGISRTGELIKIASDLDIIQKAGAWYSYNGEKIGQGSENAKKYLADNPAIFD
                     EIDHKVRVHFGMTEDDSPVQSELVEEKNEADDLVLDLDNAIEIEE"
     gene            complement(1292..2551)
                     /locus_tag="ACUBV3_000141"
     CDS             complement(1292..2551)
                     /locus_tag="ACUBV3_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HHU3846680.1"
                     /translation="MRAEIIAVGTEILTGQIVNTNAQFLSEKMAELGIDIYFQTAVGD
                     NEERLLSLLDIASQRSQLVILCGGLGPTEDDLTKQTLAKFLGKSLTVDLLASRKLDRF
                     FSSRPQFARTPNNERQAQLVEGSIPLQNLTGLAVGGIVTSKGVQYMVLPGPPSELKPM
                     VMEQVVPILSNNGTKLYSRVLRFFGIGESQLVTILEDIIKNQTDPTIAPYAKVGEVTL
                     RLSTKAENQDEADFKLDSLEKEILALKTLDNRKLKDLLYGYGDNNSMARTVLELLKVQ
                     NKTITAAESLTAGLFQSQLAEFSGASQVFNGGFTTYSMEAKSQLLGIPKKKLQEYGVV
                     SHFTAEAMAQQARQLLKADFGIGLTGVAGPDELEGYPAGTVFIGIATPEGVSSIKVSI
                     GGKSRSDVRHISTLHAFDLVRRALLKI"
     gene            complement(2640..3191)
                     /locus_tag="ACUBV3_000142"
     CDS             complement(2640..3191)
                     /locus_tag="ACUBV3_000142"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HHU3846681.1"
                     /translation="MKRCSWVNLDNPLYVAYHDKEWGRAVHDDHVLFELLCLETYQSG
                     LSWETVLNKRQEFRQVFHHYNIEKVAAMSDADLEIILQNPRVIRHRLKLFSTRQNARS
                     IILIQKEFGSFDRYIWSFVDNKVQVNSVNNYNDVPASTTLSERLSKDLKKRGFKFVGP
                     TCLYSFIQAAGMVNDHENICDFK"
     gene            complement(3214..3804)
                     /gene="ruvA"
                     /locus_tag="ACUBV3_000143"
     CDS             complement(3214..3804)
                     /gene="ruvA"
                     /locus_tag="ACUBV3_000143"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HHU3846682.1"
                     /translation="MYDYIKGKLSKITAKFIVVETAGLGYMIYVANPYSFSGYVNQEV
                     TIYLHQVIRDDAHLLFGFHTENEKEIFLNLISVSGIGPTTALAIIAVDDNEGLVSAID
                     NSDIKYLTKFPKIGKKTAQQMILDLSGKFVEASGESATSRKVSSEQNSNLEEAMEALL
                     ALGYKATELKKVKAFFEGTNETVEQYIKSSLKMLMK"
     gene            complement(3806..5038)
                     /locus_tag="ACUBV3_000144"
     CDS             complement(3806..5038)
                     /locus_tag="ACUBV3_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HHU3846683.1"
                     /translation="MKEFFALPKQLQLRELLRFISITVGSAIFPFMAMYYVQYFGNLV
                     TGILIIITQLSGFVATLYGGHLSDAMGRKKVVIIGSLLATIGWAITIAANVPNHITPH
                     LTFVGILIIEIAHQFYFPAYEAMTIDLTNEQNRRFVYTIGYWLVNIAVMLGSGIAGIF
                     YDHHFFELLIVLLIISAICCFVVYFKFDETKPQEGTFKHDKGVLGTFKNYSQVLVDKA
                     FVVYTLGAIGSSVVWLQVDNYFSVNLKQNFEVVSILGHTITGAKMLSLAVFTNTLLIV
                     LLMTTINKFIENWPLKRQLILGSLICGFGMLFNISLNTFGAILIAMTFFTFGEMIYVP
                     ASQVLRAEMMVEGKIGSYSGFLAIAQPVASVLAGAMVSLSYFTGKIGVQITLTIFMLA
                     GLVLILYATKMKNIEIGK"
     gene            complement(5070..7043)
                     /gene="mutL"
                     /locus_tag="ACUBV3_000145"
     CDS             complement(5070..7043)
                     /gene="mutL"
                     /locus_tag="ACUBV3_000145"
                     /inference="COORDINATES: protein motif:HMM:NF000950.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HHU3846684.1"
                     /translation="MSKIIELPDILANQIAAGEVVERPSSVVKELVENAIDAGSSQIT
                     IEVEESGLKKIQITDNGEGMTSEDAVLSLRRHATSKIKSQSDLFRIRTLGFRGEALPS
                     IASISLMTIKTATEQGKQGTLLVAKGGNIEKQEVVSSPRGTKILVENLFFNTPARLKY
                     MKSLQSELAHIIDIVNRLSLAHPEVAFTLINDGKEMTKTSGTGDLRQAIAGIYGLNTA
                     KKMIEISNADLDFEISGYVSLPELTRANRNYITLLINGRYIKNFLLNRSILDGYGSKL
                     MVGRFPIAVIDIQIDPYLADVNVHPTKQEVRISKERELMSLISTAISESLKQYDLIPD
                     ALENLAKTSTRSVDKPIQTSFSLKQPGLYYDRAKNDFFIGADTVSEPIANFTNLDKSD
                     GSVDNDVKNSVNQGATQSPNIKYASRDQADSENFIHSQDYLSSKQSLNKLVEKLDSEE
                     SSTFPELEFFGQMHGTYLFAQGNGGLYIIDQHAAQERVKYEYYREKIGEVDNSLQQLL
                     VPFLFEFSSSDFLQLQEKMSLLQDVGIFLEPYGNNTFILREHPIWMKEEEVESGIYEM
                     CDMLLLTNEVSVKKYRAELAIMMSCKRSIKANHTLDDYSARHLLDQLAQCKNPYNCPH
                     GRPVLVNFTKADMEKMFKRIQENHTSLRDLGKY"
     gene            7269..7472
                     /locus_tag="ACUBV3_000146"
     CDS             7269..7472
                     /locus_tag="ACUBV3_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HHU3846685.1"
                     /translation="MTQGTVKWFNSEKGFGFISSETGTDVFAHFSEIKVDGFKTLEEG
                     QKVTFDIQDGQRGPQATNINLVK"
     gene            complement(7529..10105)
                     /gene="mutS"
                     /locus_tag="ACUBV3_000147"
     CDS             complement(7529..10105)
                     /gene="mutS"
                     /locus_tag="ACUBV3_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HHU3846686.1"
                     /translation="MAKPTISPGMQQYLDIKENYPDAFLLFRMGDFYELFYDDAVKAA
                     QILEISLTSRNKNAEKPIPMAGVPYHSAQQYIDVLVELGYKVAIAEQMEDPKKAVGVV
                     KREVVQVVTPGTVVESTKPDSANNFLVAIDSQDQQTFGLAYMDVSTGEFQATLLTDFE
                     SVRSEILNLKAREIVVGYQLTDEKNHLLTKQMNLLLSYEDERLNDIHLIDEQLTDLEI
                     SAAEKLLQYVHRTQKRELSHLQKVVHYEIKDYLQMSYATKNSLDLLENARTSKKHGSL
                     YWLLDETKTAMGTRMLRTWIDRPLVSMNRIKERQDIIQVFLDYFFERNDLTESLKGVY
                     DIERLASRVSFGKANPKDLLQLGQTLSQIPRIKMILQSFNQPELDIIVNKIDTMPELE
                     SLINTAIAPEAQATITEGNIIKSGFDKQLDNYRTVMREGTGWIADIEAKERAASGIGT
                     LKIDYNKKDGYYFHVTNSNLSLVPEHFFRKATLKNSERYGTAELAKIEGEMLEAREQS
                     SNLEYDIFMRVRAQVESYIKRLQELAKTIATVDVLQSLAVVAENYHYVRPKFNDQHQI
                     KIKNGRHATVEKVMGVQEYIPNSIYFDSQTDIQLITGPNMSGKSTYMRQLALTVIMAQ
                     MGGFVSADEVDLPVFDAIFTRIGAADDLISGQSTFMVEMMEANQAVKRASDKSLILFD
                     ELGRGTATYDGMALAQSIIEYIHDRVRAKTMFATHYHELTDLSEQLTRLVNVHVATLE
                     RDGEVTFLHKIESGPADKSYGIHVAKIAGLPIDLLDRATDILSQLEADAVQLIVSPSQ
                     EAVTADLNEELDSEKQQGQLSLFEEPSNAGRVIEELEAIDIMNLTPMQAMNAIFDLKK
                     LL"
     gene            complement(10162..10599)
                     /gene="argR"
                     /locus_tag="ACUBV3_000148"
     CDS             complement(10162..10599)
                     /gene="argR"
                     /locus_tag="ACUBV3_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HHU3846687.1"
                     /translation="MNKIERQKRIKRLIQSGQIGTQEEIKLHLKNEGIDVTQATLSRD
                     LREIGLLKLRSPEGKLYYSLSTATSNRFSPALRSYILKVSRASFMLVLNTNLGEASVL
                     ANFIDEKGLPEILGTMAGADTLLVICQNEDIAKVFEKELSVGL"
     gene            10821..12512
                     /gene="argS"
                     /locus_tag="ACUBV3_000149"
     CDS             10821..12512
                     /gene="argS"
                     /locus_tag="ACUBV3_000149"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HHU3846688.1"
                     /translation="MDTKHLIASEIQKVVPDMEQSTILSLLETPKNSSMGDLAFPAFS
                     LAKTLRKAPQIIASDIAEQIKSDQFEKVEAVGPYVNFFLDKAAISSQVLKQVLSDGSA
                     YATQNIGEGRNVAIDMSSPNIAKPFSIGHLRSTVIGDSLANIFDKIGYHPVKINHLGD
                     WGKQFGMLIVAYKKWGNEEAVRAHPIDELLKLYVRINAEAETDPSVDEEAREWFRKLE
                     ANDPEATELWQWFRDESLLEFNRLYDQMNVTFDSYNGEAFYNDKMDEVLELLESKNLL
                     VESKGAQVVNLEKYGIEHPALIKKSDGATLYITRDLAAALYRKRTYDFAKSIYVVGNE
                     QSAHFKQLKAVLKEMDYDWSDDMTHVPFGLVTKGGAKLSTRKGNVILLEPTVAEAINR
                     AASQIEAKNPNLADKDKVAQAVGVGAIKFYDLKTDRTNGYDFDLEAMVSFEGETGPYV
                     QYAHARIQSILRKANFSPSNSDNYSLNDVESWEIIKLIQDFPRTIVRAADNFEPSIIA
                     KFAINLAQCFNKYYAHTRILDEDAEISSRLALCYATATVLKESLRLLGVDAPNEM"
     gene            12600..12908
                     /locus_tag="ACUBV3_000150"
     CDS             12600..12908
                     /locus_tag="ACUBV3_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HHU3846689.1"
                     /translation="MPSEKEILDALSKVYSEEVIQADDYFRQAIFELASQLEKEGMNS
                     LLATKIDSLINQYVLTHQFDAPKSIFDLSRLVKTKASHYKGTAISAIMLGSFLSGGPK
                     "
     gene            complement(12935..13807)
                     /locus_tag="ACUBV3_000151"
     CDS             complement(12935..13807)
                     /locus_tag="ACUBV3_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HHU3846690.1"
                     /translation="MLKLDLKTKIKETILIAFGVALYTFGFVKFNMANHLAEGGISGV
                     TLIIHALFGVNPALSSLLLNIPLFILGARILGKKSLLLTIYGTVLMSFFMWFWQQIPV
                     TVPLKNDMMLVAVAAGILAGTGSGLVFRYGATTGGADIIGRIVEEKSGIKLGQTLLFI
                     DAIVLTSSLVYINLQQMLYTLVASFVFSQVLTNVENGGYTVRGMIIITKESESAAATI
                     LHEINRGVTFLRGQGAYSGREHDVLYVALNPSEVRDVKEIMADLDPDAFISVINVDEV
                     ISSDFKIRRRNYDK"
     gene            complement(13915..14859)
                     /locus_tag="ACUBV3_000152"
     CDS             complement(13915..14859)
                     /locus_tag="ACUBV3_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HHU3846691.1"
                     /translation="MKTRRTPLEKKVKYIISVWAKKFGLLHTLKSISREKYAEKISAS
                     LLYGILSSVAVNFFFQPGHVYSSGATGLAQVISAVSKHWFSFEIPVALAFYAINIPLL
                     ILSWRKIGHKFTIFTFITVTVSSIFIQLMPQITLTTDPLINAIFGGLIMGAGVGFSFK
                     SRISSGGTDIISLTIRKKTGRDVGSISFIINGIILLFAGLLFGWKYALYSMVTIFVSS
                     RVTDAIFTKQKKMQAMIVTSKPDCVIKRIHRDLHRGVTCINDAEGTYNHEKKAVLITI
                     LTREEFSDFKYLMLKADPKAFVSVAENVHIIGRFVDDD"
     gene            complement(14849..16600)
                     /gene="aspS"
                     /locus_tag="ACUBV3_000153"
     CDS             complement(14849..16600)
                     /gene="aspS"
                     /locus_tag="ACUBV3_000153"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HHU3846692.1"
                     /translation="MKRSMYAGRVRSEHIGTSITLKGWVGRRRDLGGLIFIDLRDREG
                     IMQLVINPEEVSASVMATAESLRSEFVIEVSGVVTAREQANDNLPTGEVELKVQELSI
                     LNTSKTTPFEIKDGIEANDDTRMRYRYLDLRRPEMLENFKLRAKVTHSIRNYLDNLEF
                     IDVETPMLTKSTPEGARDYLVPSRVNQGHFYALPQSPQITKQLLMNAGFDRYYQIVKC
                     FRDEDLRGDRQPEFTQVDLETSFLSDQEIQDIVEGMIAKVMKDTKGLEVSLPFPRMAY
                     DDAMNNYGSDKPDTRFDMLLQDLTEIVKEVDFKVFSEASVVKAIVVKDKADKYSRKNI
                     DKLTEIAKQYGAKGLAWLKYADNTISGPVAKFLTAIEGRLTEALQLENNDLILFVADS
                     LEVANETLGALRTRIAKELELIDYSKFNFLWVVDWPMFEWSEEEGRYMSAHHPFTLPT
                     AETAHELEGDLAKVRAVAYDIVLNGYELGGGSLRINQKDTQERMFKALGFSAESAQEQ
                     FGFLLEAMDYGFPPHGGLAIGLDRFVMLLAGKDNIREVIAFPKNNKASDPMTQAPSLV
                     SEQQLEELSLTVESYEN"
     gene            complement(16693..17973)
                     /gene="hisS"
                     /locus_tag="ACUBV3_000154"
     CDS             complement(16693..17973)
                     /gene="hisS"
                     /locus_tag="ACUBV3_000154"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HHU3846693.1"
                     /translation="MKLQKPKGTQDILPGESAKWQYVENVIRNLFKQYHYDEIRTPMF
                     EHYEVISRSVGDTTDIVTKEMYDFHDKGDRHITLRPEGTAPVVRSYVENKLFAPEVQK
                     PTKMYYIGSMFRYERPQAGRLREFHQVGVECFGSNNPATDVETIAMGHHLFEDLGIKN
                     VKLHLNSLGNPESRQAYRQALIDYLTPIREQLSKDSQRRLNENPLRVLDSKEPEDKLA
                     VENAPSILDYLDESSQAHFDAVCHMLDALNIPYIIDTNMVRGLDYYSHTIFEFITEIE
                     DNELTICAGGRYDGLVSYFGGPETPAFGFGLGLERLLLILDKQGISLPIENTIDLYIA
                     VLGSEANLAALDLAQSIRHQGFKVERDYLGRKIKAQFKSADTFNAKVIMTLGSSEVDS
                     KEVGLKNNQTRQEVKVSFENIKTDFSSVLKQLGL"
     gene            18193..18375
                     /gene="rpmF"
                     /locus_tag="ACUBV3_000155"
     CDS             18193..18375
                     /gene="rpmF"
                     /locus_tag="ACUBV3_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HHU3846694.1"
                     /translation="MAVPARHTSKAKKNKRRTHYKLTAPSVQFDETTGDYSRSHRVSL
                     KGYYKGRKIAKANEAK"
     gene            18391..18540
                     /gene="rpmG"
                     /locus_tag="ACUBV3_000156"
     CDS             18391..18540
                     /gene="rpmG"
                     /locus_tag="ACUBV3_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HHU3846695.1"
                     /translation="MRVNITLEHKESGERLYLTSKNKRNTPDRLQLKKYSPKLRKHVV
                     FTEVK"
     gene            complement(18688..19209)
                     /locus_tag="ACUBV3_000157"
     CDS             complement(18688..19209)
                     /locus_tag="ACUBV3_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HHU3846696.1"
                     /translation="MRTYSDKNELKEEVLKSYKKYIAEFNDIPEKLKDLRIDEVDRTP
                     AENLAYQVGWTTLILKWESDEQSGLEVKTPTETFKWNQLGELYQHFTETYASLTIKEL
                     TAQLNDNVDAIGNMIDSMSDEVLFKPHMRNWADSATKNAVWEVYKFIHINTVAPFGTF
                     RTKIRKWKKVALK"
     gene            complement(19307..20791)
                     /locus_tag="ACUBV3_000158"
     CDS             complement(19307..20791)
                     /locus_tag="ACUBV3_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HHU3846697.1"
                     /translation="MRDNGSRVLIISSWRPDPYKAGNVLVKFAMRFTHPITKKSHKKY
                     LSTGASKGWFTTKATPSKKLPSGKERLLVSDIKNTQLITQVTQELNKLVDDYIAELMG
                     IKPKKAKKLLTLEEIAKPFDKDGNFYGKAFKAWHERVKPANNTLKTRVTIYNRYIEPN
                     FDTRMSITKFAFMTDEIQNLINASSMHMARNLHIYLKMIFDWSVENGQITLTQDPIAS
                     NKVKRRVLTKSEEQDKKREDIAEKYLEASEVNHVLRLIESWTNRPDNQLIADVLRMIF
                     LTGMRPSEVLGLNEDMLDFEKKWIKVHWQRASKNKSDDMMEALNLDEKERYRADLKTK
                     ESVRTIPMSPEVEKILRHYIDRNKFQAQFSPTYQDLGYLFTRTYIRAGNRQGSPLYHN
                     ELSQFLRGGSSQSAKYNKKAGKPYKDIDSFLDFGRPIHVIPHMFRHSFISIMASEGID
                     LPTIREFVGHSEDSKEIERVYLHVIKKQKDTMRGAVEKLEKLIE"
     gene            complement(20801..21049)
                     /locus_tag="ACUBV3_000159"
     CDS             complement(20801..21049)
                     /locus_tag="ACUBV3_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018164767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3846698.1"
                     /translation="MATLKTEHDFDLTRNWYRKSVFLDELWHGMTVATLNSYIRQMKD
                     SPYAFGIKGTHGNVFIHSEVFVAWFDYKIANQYVAKMA"
     gene            complement(21115..22143)
                     /locus_tag="ACUBV3_000160"
     CDS             complement(21115..22143)
                     /locus_tag="ACUBV3_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HHU3846699.1"
                     /translation="MDNSVMLDYLAVTIKGLAPDDVIEKILILPKDKFVLNEWGINKY
                     QRHYSFSEIKVYFNKDWQSKMGVFIELRGQGCRQYEEYMENNVNNWVTLMKRISECHS
                     NVTRLDIANDIFDDSLSVPLIYSYCKKQLCISTAKTFDYHEKSLLENGEKVGEMVTIG
                     VRGTQQWCVYNKLLEQKLDQELPNTPLSWTRAELRCWQEKANLLAKQIKEGRPLKEIY
                     FEVINGHYRFVSPRDKDSNRWRRKTVKWWNDYLETQEKTVLSVKRTKPTLKRSEKWTE
                     KQVSRTLGKLYVAKAESHGQEKADSYIQHLLELGISKLTETDETDIQQYKQEQLSSAY
                     WGIRKDDL"
     gene            complement(22146..22577)
                     /locus_tag="ACUBV3_000161"
     CDS             complement(22146..22577)
                     /locus_tag="ACUBV3_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3846700.1"
                     /translation="MTKFNLTDETIKVGDLKLDKSQFDIPKKVTILSSRVAPVYKNVN
                     GESIATDEVAKITCSVQDTDKIQALKDLGYSSDDLKGIRLEIVDNLDKLAKSVEKDGL
                     NSLVVELVNPEVHLGWRANNNGGGNWGGLKLVATDIKFIGA"
     gene            complement(22647..22856)
                     /locus_tag="ACUBV3_000162"
     CDS             complement(22647..22856)
                     /locus_tag="ACUBV3_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000860007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3846701.1"
                     /translation="MKTVRHPTAGRVVNTVAQSRWGRWQTAEIEIEIAPRSLLGGAHI
                     HEMWSKPLISKGFSDLKQISRKKSL"
     gene            complement(22853..23308)
                     /locus_tag="ACUBV3_000163"
     CDS             complement(22853..23308)
                     /locus_tag="ACUBV3_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001008113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3846702.1"
                     /translation="MNEYDYQEVVDRVDGLNSVSTLKKWRLKIESLTGTQFKESRVRT
                     GRKSYSKIYLFTDDDLAYFQAVADKKSSLGLERAILSVYGSSKGKDHQKPIRELVDEL
                     EVSFMEIQEDYQRNLTELKQKLEVLLVRIQNLEKETGDKTSKRIGFFKR"
     gene            complement(23308..23523)
                     /locus_tag="ACUBV3_000164"
     CDS             complement(23308..23523)
                     /locus_tag="ACUBV3_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3846703.1"
                     /translation="MNQTSIELQMVEWVAYSPETQEKVFIKPEDCKDLDDRELYHLIK
                     STGFERLIESRALQIVTDFLNQKTQVS"
     gene            23846..24766
                     /locus_tag="ACUBV3_000165"
     CDS             23846..24766
                     /locus_tag="ACUBV3_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HHU3846704.1"
                     /translation="MFEILKPDKKAVGKRLRQVKDELNLSFTDFGNRLGLKKSTINAY
                     VRGDNLAPLEVLEKVSKIYGKPVGWFYYGELEEYIELYLRKLGYGTFLDEYPETPLKI
                     KDELFRIKANPKQLITTNGGVTIGTFENPNWENDFGYPNEGYLDDIFFEMLCETLKNS
                     IRQEAQTYLSQQEGLDDTKIKELSNYFANQIYISYDFVGNLENLSKDKIKQEVKESYE
                     RLLKDSPQAGVEVSYDSNYLLGFLINTLADSQTTRQLLSDMSQATVGMPFSQFHEGHE
                     LVEVIQSVRPRLIELYNNTTFDDWTDWAEK"
     gene            24787..25908
                     /locus_tag="ACUBV3_000166"
     CDS             24787..25908
                     /locus_tag="ACUBV3_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HHU3846705.1"
                     /translation="MDNKFKNIPKRHLPQKLPFDYTSIYTDPQIIKLTTRANIAIGTY
                     EGFLESIINPMLLISPLLSQEAVLSSKLEGTHATLKDLLNYEAGNKVDIERDELHEII
                     NYRKALFYALENISTINNIDSKGLPLSNRIIKEMHKILLDNVRGSSKNPGNFKRSQNY
                     IGSVSSISYTPVPAEKTPEYMSNLEQYIHYDDLDLLVQSAIIHAQFEMIHPFEDGNGR
                     IGRLLIPLFLYYQELLSYPTFYMSSYFERDRSLYISHLSNISKDNNWKDWFEYYLEGV
                     ILSAEESTKKAQDILSLYNIMKEQVIPKLNSVSGIQLLDFIFSAPIFKAEQVSEHLKI
                     SKRTTYTLLNKLIDEGYLSTDNAQRNRTYYCPQLLSIVQ"
     gene            26136..26945
                     /locus_tag="ACUBV3_000167"
     CDS             26136..26945
                     /locus_tag="ACUBV3_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3846706.1"
                     /translation="MPKNRINNFINIGGLILVISFLLLVLDIVNLPTKFGFNIKTLNI
                     EIWNIYATLLGSGLTLYGVIITINSQYESQIEDTKRIVKPMINVSPVDEYSYKDKYIQ
                     FDFIFPKAYPNLLRKDNIDTPNVTISLENLGTRELYNLYIGNFEATYFDDGGYYYQLN
                     PILYSNHKVQLNLMFYEKNGYLKEFNQEDKFHLLISPIKFSIYFQDCLNNWYEQSFKL
                     RIFHQVQADRSDEENSLTVSIERVEIYSAPKEISPDSLPWITNPSKLCQFG"
     gene            complement(27228..27412)
                     /locus_tag="ACUBV3_000168"
                     /pseudo
     CDS             complement(27228..27412)
                     /locus_tag="ACUBV3_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002892741.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FanG protein"
     gene            27441..28055
                     /locus_tag="ACUBV3_000169"
     CDS             27441..28055
                     /locus_tag="ACUBV3_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HHU3846707.1"
                     /translation="MIQNVVTSIILYSGTAVDLLIILMLFFAKRKSRKDIINIYLGQF
                     LGSVSLIFLSLLFAFVLNYIPSKEILGLLGLIPIFLGLKVLLLGDSDGEAIAKDGLRK
                     DNKNLIFLVAMITFASCGADNIGVFVPYFTTLNLANLIVTLLTFLVMIYLLVFSAQKL
                     AQVPSVGETLEKYSRWFIAVVYLGLGMYILIENNSFDMLWAVLG"
     gene            28067..28405
                     /gene="cadX"
                     /locus_tag="ACUBV3_000170"
     CDS             28067..28405
                     /gene="cadX"
                     /locus_tag="ACUBV3_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cd(II)/Zn(II)-sensing metalloregulatory
                     transcriptional regulator CadX"
                     /protein_id="HHU3846708.1"
                     /translation="MKKDSICQVDVINQQNVTTATNYLEKEKVQKSLRILSKFTDNKQ
                     INIIFYLLAVEELCVCDIACLLNLSMASASHHLRKLANQNILDTRREGKIIYYFIKDE
                     EIRDFFNQLG"
     gene            28456..29871
                     /locus_tag="ACUBV3_000171"
     CDS             28456..29871
                     /locus_tag="ACUBV3_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000667635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HHU3846709.1"
                     /translation="MKFFEENYSQEIPTRIKNLRKKYNITQSELGNAGQVSQVESGKR
                     PITSSMLVYLNALTASSYTYIVFGELDEFIENLFHYFFSSILYRDLEAVDEKLYSFMS
                     DDLISIQSSCLSIAKTFANFNIQRKRFMISTETEMDTFHKKDDIDVWVGGKSDNPARS
                     FRTRTINELTVIDFEEMFDILWLMLGDNLIKSFEVNVCGILFELGGNDIPSTFRQENI
                     DPLINKWWYDNVSTEIIPNLIKKLKENPLFNIGFMVNDILERMYKENIPKSYLTSVPL
                     VISQKGRTTYSFSMTGGQQIDGVKFTQIYEDYMKLLSQGKDIAELYQKYSKEELANLG
                     INIYQSNDIERTEERTFDEIISWVSNPYATRPIQERHTIQLEPTRFSLEDKKRIEEAA
                     AQGLSEIDLIDLVDLYDINLDNTSVNRHIVGLLTNNTQVTYYFQEQLNKELLSMAHAL
                     DNVQQAFIKLLSEEEIRKFAL"
     gene            30192..30641
                     /locus_tag="ACUBV3_000172"
     CDS             30192..30641
                     /locus_tag="ACUBV3_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004189491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HHU3846710.1"
                     /translation="MSNKVQERRERKIKEAIKAKNWDEVTRLLQQEQSNAERRDRYHN
                     RRIKDETIASKNAKKSVRYDVIASSDLNPEEALILEELRQAIREAKASLSEIDSKIVE
                     MIAEQGSSYKETARYITEHYKKMSDVTVKSHYCKALKKLAPLLKAYR"
     gene            30843..31163
                     /locus_tag="ACUBV3_000173"
     CDS             30843..31163
                     /locus_tag="ACUBV3_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003032314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3846711.1"
                     /translation="MAFKMKTTKGGYTTTLADKIISQKQPIYSLSTELEPQQRFEEGK
                     PTGEIVAYKAWFVQEGQDPFQVKFEETIELPAFQSMIQFDTLQACEVKYNIYFKANGI
                     KEVR"
     gene            31163..32263
                     /locus_tag="ACUBV3_000174"
     CDS             31163..32263
                     /locus_tag="ACUBV3_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system DNA-binding
                     domain-containing protein"
                     /protein_id="HHU3846712.1"
                     /translation="MTSQSRDVLNQSYLQSYLLQSLNMALGALMQGETSYTNSFNIVI
                     QADGFIFVPRLPCAYILDDDLYKKIFLIANASLYPQYTLLKQNATYFVPLETDDLHTQ
                     RGLFFPWKKGISERLVIPDLDKFSASLPQGKIPIMKYFELNLDKVNHWAIAGNSGSGK
                     SYALTYFLSVLKHMSDLIIIDPKFDTPSRWARENHVAVIHPVENRSKSDFVSQVNEQL
                     SQCATLIQKRQAVLYDNPNHQFTHLTIVIDEVLALSEGVNKNIKEAFFSLLSQISLLG
                     RATKIHLFLVSQRFDHNTIPISVREQLNVLLQIGNINQKTTQFLFPDLDPEGIVIPIG
                     HGTGIIQVIDNEHPYQVLPLLCPTYYTKRGIL"
     gene            32260..32865
                     /locus_tag="ACUBV3_000175"
     CDS             32260..32865
                     /locus_tag="ACUBV3_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3846713.1"
                     /translation="MTNKTYFMRSFNLILYLFLFAPICSAFGWLFNYLSVQLTNITFV
                     NLDTVKSITDIISSVCHGFALISLLLALFLVGLEIIQRLKTDSLWNYIQSVYHTFLLR
                     HFLFQRERVQKISNLEHQTVTTINPIHNGFNRAVRKCIVDIRKDTITIFLKVPRDQQG
                     QKILKEMEAQLKEEIVSQHADYYFSSPIRVRNQLWFTGQKR"
     gene            33071..33676
                     /locus_tag="ACUBV3_000176"
     CDS             33071..33676
                     /locus_tag="ACUBV3_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003032321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HHU3846714.1"
                     /translation="MAKEQRSTKWTFLFYEESAPENYLNILEELHIPFILSPWHDKDV
                     NRQTGEFKKSHKHGAFFFDSLKSYSQVSNIISDKLNGPAHVEVVMSPTGLFDYFTHAE
                     NPDKTPYNIEDIEVGCGFNLEKFLMEMNSSDFIHEVVDIIEENDFTEFEELVWYARAN
                     NTNLLGLIIERTYFFAKYLDSRRYNPNRLHNSNTEEKENNE"
     gene            33669..33917
                     /locus_tag="ACUBV3_000177"
     CDS             33669..33917
                     /locus_tag="ACUBV3_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003009477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HHU3846715.1"
                     /translation="MNNEKLEQIEQLLQTLIKLIQIKEQNIPLKIIQQRELLKQLNIS
                     PNTLKTWEQKGLKRLEPPIEGTRTVFYLLDDIINFLQS"
     gene            34007..35272
                     /locus_tag="ACUBV3_000178"
     CDS             34007..35272
                     /locus_tag="ACUBV3_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HHU3846716.1"
                     /translation="METETIIHNGKKVIKKIKKDKSISYTLKGTFLGKDIKTGKQVTT
                     TITAKTLKQLDRAIIQARLEFEKNGSTREKVIVIDTLEDLAEEWFKSYKTWVNSHNTL
                     NRVRGYLDNYIIPKFGDYKPDKIESADIQLWLNDLAKKSKESIESGIKRADKGSAKDF
                     GAVIHKLKDIFDYGITNYGLITNPVSTVKIPPKPKSNKQRIMVLHDDGLVIWLNYLES
                     LDNNRANRRFKLICNTLLASALRINELLALTIDDLDFENSSINVSKTLMWKTANKKDG
                     TKGEVICKATPKTDAGNRSVPVPPPIIEKLRDFHHEMNKYLELHNRHRTKLIFPTIYG
                     NYMCDRNERTTLKKRLVGLGLPNYGFHLFRHTHASMLLNAGTNWKELQVRMGHKSIST
                     TMDTYAELAPQRKLEAVGIYLEKIAELTQ"
BASE COUNT        11269 a         6970 c         5787 g        11330 t
ORIGIN      
        1 gtataaaaat aagttccaac cactgtcaga acttatttta gttacattta ttcaagttca
       61 agatgtatag ttgctatttt attcctcaat ttcaatagca ttgtccaaat ctaaaacaag
      121 atcatcagct tcattttttt cttcaacgag ctcagactga actggcgaat catcttcagt
      181 cattccaaag tgcacgcgca ctttatggtc aatttcatca aaaattgctg gattatctgc
      241 taaatatttt ttggcatttt cagaaccttg tccaattttc tcaccattat atgagtacca
      301 tgcacctgct ttttggataa tatccaaatc actagcaatt ttaattaact cacccgtgcg
      361 agaaatacct tcaccataca taatttcgac aaaagcttca cggaatggtg gagcaacttt
      421 atttttaaca actttgattt ttgtctcttt accaacatta tgatctttat gctcaccagt
      481 tcccttgatt tgagtattac cacgaacatc tagacgaacc gatgaataaa atttcaaagc
      541 acgaccacca ggtgttgttt cagggttacc aaacataacg cctacttttt cccttaactg
      601 gttgataaaa attgcgattg tctttgtctt gttaatggat gctgacaatt tacgcattgc
      661 ttgactcatc atacgagctt gcaaaccaac gtggctgtca ccaatatcac catcaatctc
      721 agcacgagga acaagggctg caactgaatc gacaacaact aaatcaactg cgcctgaatc
      781 aatcaattta cctgcaattt caagaccttg ctcacctgag tcaggttgag acaaaagtaa
      841 ctcatcaata ttaactccaa gagccgcagc ataggctggg tcaagagcgt gctcagcatc
      901 aataaaggca gcaatcccac cttccttttg ggcttgagct acggcatgaa gggcaaccgt
      961 tgttttacca gatgactctg gtccataaat ttcgacgata cgtcctttag gatatccacc
     1021 tgctcctaaa gcgatatcta aagcaagaga acctgaactc attacttgaa ccttttgttc
     1081 agctcgctca cctaagcgca tcactgcgcc cttaccaaaa tctttttcaa tatttttcaa
     1141 agcatcatct aaggcttttc gacgttcatc accgaatttc ttagtaattt cttctgcttt
     1201 tttcgttttt ttagccaatg tcttctcctt atatttctat gaatctaggt tgattcaaat
     1261 ttagaaatcg ttctactaat tataacaaat attatatctt taataaagcc ctacgcacca
     1321 agtcaaaagc atgtaaagta ctgatatgac ggacatctga gcgactcttc cctccaatcg
     1381 atactttaat agaagaaacg ccttcagggg ttgcaatccc aataaaaact gtacctgcag
     1441 ggtatccttc caactcatca ggaccagcaa ctcccgttaa accgatacca aaatctgctt
     1501 ttaaaagttg gcgagcttgc tgagccattg cctcagctgt aaaatgactg acaactccat
     1561 attcttgcaa ttttttctta gggatgccga gaagttgtga ttttgcttcc atactatagg
     1621 tcgtaaaacc accattaaaa acttgcgaag caccagaaaa ttctgctaac tgagattgaa
     1681 ataaccctgc tgttaagctc tctgcggcag taatcgtttt attttgtact ttcaataatt
     1741 ctaaaacagt acgtgccata ctattattat caccataacc atataataaa tcttttaatt
     1801 tacggttatc taatgtctta agagcaagta tttctttttc taagctatct aatttaaagt
     1861 cggcctcatc ctgattttca gctttcgttg ataaccttaa agttacctca ccaacctttg
     1921 catatggtgc tatagttggg tctgtctgat tcttaattat gtcttctaag attgtgacta
     1981 actgactctc accgatacca aagaatctca atacacgtga atataatttt gtgccattat
     2041 ttgacaaaat aggtactacc tgctccataa ccattggctt taattcacta ggcggacctg
     2101 gtaaaaccat atattggact cccttagatg taacgatacc accaacagct agaccagtaa
     2161 ggttttgtag aggtattgag ccttcaacca attgagcttg tcgttcatta tttggcgttc
     2221 tagcaaactg aggacgactt gaaaaaaaac gatccaattt tcgactagct aataaatcta
     2281 ctgttaatga tttacctaaa aatttagcca aagtttgctt cgttaagtca tcctctgtag
     2341 ggccaagtcc accacatagg ataaccaatt gactgcgctg actagctata tctaaaagcg
     2401 ataataaacg ttcctcattg tctcctacag ctgtctggaa ataaatatca attcccaatt
     2461 ctgccatttt ctccgacaaa aattgtgcgt ttgtattaac gatctgtcct gttaaaattt
     2521 ctgttcctac agcgataatt tcagccctca caacttcacc ctcttatcta ttcgaaataa
     2581 tgactacatt ttagcaaaaa aagtagcaaa agtgctactt aaaacatatc acattgatat
     2641 tacttgaaat cacatatatt ttcatggtca ttaaccatac ccgcggcctg aataaaagaa
     2701 tagaggcaag ttggaccaac aaatttaaaa ccgcgttttt ttaaatcctt agataagcgt
     2761 tcggataagg tagtacttgc aggcacatcg ttgtagttgt taactgagtt aacctgtact
     2821 ttattatcta caaaagacca aatgtagcga tcaaaacttc cgaattcttt ctgaattaat
     2881 ataattgacc tagcattttg tcgtgttgag aaaagtttta gacgatgacg aataacccta
     2941 ggattttgta agattatctc taaatccgca tctgacattg cagctacctt ttcaatatta
     3001 tagtgatgaa aaacttgtct gaactcctga cgtttattta atactgtttc ccaagataaa
     3061 cccgattgat aagtttctaa acatagtaac tcaaagagta catgatcgtc atgaacagct
     3121 ctcccccact ctttatcatg gtaagctaca tataatggat tatctaaatt cacccaagaa
     3181 catcgtttca tgtgaaacct cattacttct aaattatttc atcaacatct tcaaactaga
     3241 tttgatgtat tgctccacag tctcgttcgt accttcaaaa aaggctttta cttttttcaa
     3301 ttcagtcgcc ttatacccca acgctaacaa agcttccata gcttcttcaa gattgctatt
     3361 ctgctcagag ctaaccttac gactagtcgc actctcacca gaagcttcta caaacttccc
     3421 tgacaaatca aggatcatct gttgtgctgt tttttttcct atctttggaa atttagtcaa
     3481 atacttaata tcactattat caatggcact tacgagacct tcattgtcat cgactgcaat
     3541 aattgctaaa gcggtcgttg gaccaattcc tgatactgaa attaaattaa gaaaaatttc
     3601 tttttcattt tccgtatgaa aaccaaataa aagatgtgca tcatcgcgaa tgacttgatg
     3661 taagtagatg gtaacttctt gattaacgta acctgaaaaa gagtagggat ttgccacata
     3721 tatcatgtat cccaatccag ctgtttccac aacaataaat ttagccgtta ttttactcaa
     3781 ttttccttta atataatcgt acattctatt ttcctatctc aatatttttc attttggtag
     3841 catataagat taaaaccaga cctgctaaca taaaaatggt caatgttatt tgaacaccta
     3901 tttttcctgt aaaatagctg agcgaaacca tggcacctgc taatacactt gcaacaggtt
     3961 gtgcgatagc taaaaatcca gaatatgatc caatttttcc ctccaccatc atctccgccc
     4021 ttagaacctg actagctgga acataaatca tttcgccaaa ggtgaaaaac gtcatggcaa
     4081 tcaaaatagc tccaaaagtg tttagagaaa tattaaaaag cattccaaaa ccacaaatca
     4141 agctccctaa tattagttga cgtttcaaag gccaattctc tatgaattta tttatagttg
     4201 tcattagtaa gacaattaat agagtgtttg taaagactgc taaactaagc attttagcac
     4261 ctgtgattgt atgtcctaaa atggacacta cttcaaaatt ttgcttaaga ttgaccgaga
     4321 aataattatc aacctggagc cagacaacac tggaaccaat tgctcctaaa gtataaacaa
     4381 caaacgcttt atccactaaa acctgactat aatttttaaa agttcctaaa actcctttat
     4441 catgcttgaa ggttccctcc tgtggttttg tctcatcaaa cttgaagtag accacgaaac
     4501 aacaaatcgc tgaaataata agaagaacta ttaataattc aaaaaaatgg tgatcataga
     4561 aaatcccagc aattccagaa cctagcatta cagcgatgtt aaccaaccaa taacctatag
     4621 tgtaaacaaa gcgacgattc tgttcgttcg tcaaatcaat ggtcatagct tcataggcag
     4681 ggaaataaaa ttggtgagct atttcaatga taaggatacc tacaaaggtc aagtgtggcg
     4741 taatatgatt tggaacattt gcagctatag ttatagccca accaatggtt gccagcagtg
     4801 aacctataat cacaactttt ttcctcccca tagcatctga caaatggcct ccatataagg
     4861 tcgctacaaa ccctgatagt tgagttatga tgatcaagat tcctgtaact agatttccaa
     4921 aatattgaac ataatacatt gccataaaag gaaaaatagc tgaaccaacc gtaatggaaa
     4981 taaaacgcaa taattctctt aactgtagct gttttggcaa agcaaaaaac tctttcactt
     5041 tttctccctt cttccctaac aagtaattat caatattttc ctaaatccct gaggcttgta
     5101 tgattttctt ggattcgttt aaacattttc tccatatcag cttttgtaaa attaactaaa
     5161 acaggtcttc catgagggca attataagga tttttacatt gcgctaactg atctaataaa
     5221 tgacgtgctg agtaatcgtc tagcgtatgg tttgctttaa tagatcgctt gcaagacatc
     5281 ataatagcca actctgcgcg atattttttc actgatactt cattagtgag caacaacata
     5341 tcacacattt cataaatgcc agactctacc tcttcttctt tcatccaaat tggatgttcc
     5401 cttaaaatga aagtattatt accatacggt tccaagaaaa taccaacatc ttgtaataat
     5461 gacatttttt cttgtaattg aagaaaatct gaacttgaaa attcaaatag aaatggtact
     5521 aacaattgtt gaagactatt atcaacctca cctatctttt cacggtaata ctcgtatttt
     5581 acacgctctt gagctgcatg ttgatcaata atatacaacc caccattccc ttgagcaaat
     5641 aagtaggtcc catgcatttg accaaaaaat tcgagctctg gaaaagtcga actttcttcc
     5701 gaatctaatt tttcaacaag tttgttaagt gactgtttac tactaaggta atcctgagaa
     5761 tgaatgaagt tctcactatc agcttgatct ctactagcat actttatatt aggagactgc
     5821 gttgctcctt gattaacaga gttttttaca tcattgtcaa ctgaaccgtc acttttgtca
     5881 aggtttgtaa agttagcaat aggttcagat acagtatctg cacctataaa aaagtcattt
     5941 ttagccctat cgtaatacaa accaggctgt tttagtgaga agcttgtttg tataggctta
     6001 tctacacttc gggtactagt tttagctaaa ttctctaaag catccggaat caagtcatat
     6061 tgtttaagac tctcagaaat tgctgtgctt attaaactca ttaattctcg ttccttagaa
     6121 attctcacct cttgcttagt cggatgaacg ttgacatctg ctagataagg atctatctga
     6181 atatcaatga cagcaattgg aaatcttcca accatcaact ttgaaccata accatctaga
     6241 attgagcggt tcaaaagaaa atttttaatg taacgaccgt taattaatag ggtaatatag
     6301 tttcggttag ctcgtgtcaa ttcaggcaaa cttacataac ccgaaatttc aaaatctaaa
     6361 tctgcatttg aaatctcaat catttttttt gcagtattta aaccataaat acctgcaatc
     6421 gcctgtctca aatcgccagt cccactagtt ttagtcattt ctttaccatc atttatcagt
     6481 gtaaaagcaa cttccggatg cgctagactg agtctattaa caatatcaat aatatgagct
     6541 agttcagact gtaaactctt catgtatttc aatctggcag gcgtgttaaa gaataaattt
     6601 tcaactaaaa tttttgttcc acgaggactt gaaacaactt cctgtttttc aatgtttccc
     6661 cctttagcaa caagtaacgt cccttgtttc ccttgctctg tagctgtttt tatcgtcatt
     6721 aaactaatcg aagcgattga tggtagagct tcaccacgaa aacctaaggt ccgaatacga
     6781 aaaaggtcac tctggctttt gattttggat gtagcgtgac gtcgtaaact gagaacggca
     6841 tcctcactag tcatcccttc accgttatcc gttatttgaa tctttttaag accagattct
     6901 tcaacctcta tggtaatttg gctacttcct gcatctatgg cattttcaac taattcttta
     6961 acgacgctac taggcctttc aacaacctca ccagcagcaa tctggttagc taatatatcc
     7021 ggtaattcaa taattttaga caagttcatc cctccttttt ttatgatttt ttgtaacttt
     7081 ttttgtgata tcttagccat tataccatat tcaaaatatt aatttgacaa taacacaatt
     7141 tttagttgac atcactccta ttacctgtta gacttaggga gcgtaataag ttttgaagtt
     7201 gtatttttgt ttaacatttt tacctttttt attttttaca gtaacatttt tagaaaggta
     7261 ataaacaaat gacacaaggt acagtgaaat ggtttaactc agaaaaaggt tttggcttta
     7321 tttctagcga aactggaaca gacgtcttcg ctcatttttc agagattaaa gttgatggct
     7381 tcaaaactct tgaagaagga caaaaagtaa catttgatat tcaagatggt caacgtggac
     7441 cacaagcaac aaatatcaat cttgttaaat aattaaaaaa gagcctaaat gaattattta
     7501 gtctcttttt tgttgcctaa aggctatttt ataataattt ctttaagtca aatatagcat
     7561 tcatagcttg cattggagtt agattcatta tatctatcgc ttctaactcc tcaataaccc
     7621 tacctgcatt tgaaggttct tcaaaaagcg ataattgtcc ttgttgcttc tcagaatcta
     7681 gttcctcatt taagtcagca gtaacagctt cttgggaggg cgatacgatc aactgtactg
     7741 catcagcttc caactgtgat aaaatatcag ttgccctatc caataagtca attggtaaac
     7801 cagctatttt tgcgacgtgt atcccataag acttatccgc aggtccagat tcaattttat
     7861 gtaagaaggt aacttctcca tctctctcta aagtagccac gtgtacattg acaagccttg
     7921 tcaactgttc agataaatct gtcaactcat ggtaatgagt cgcaaacatt gtttttgccc
     7981 taacacggtc atgaatatat tctataatcg attgagctaa tgccatacca tcataagtgg
     8041 ctgtccctcg acctaattca tcaaaaagaa tcaaagattt atcactggct cgttttacag
     8101 cttgattcgc ttccatcatt tctaccataa aggttgattg cccagaaatt aagtcgtcag
     8161 cagcaccaat cctagtaaat attgcatcaa atacaggcaa atcaacttcg tctgccgata
     8221 caaaacctcc catttgtgcc ataataactg tcaaagctaa ctggcgcata taggtcgact
     8281 taccactcat atttggtcct gtaatcaact ggatatctgt ctgactatca aaatagatgc
     8341 tattgggaat atattcttgc actcccatca ctttttcaac agttgcatga cgcccattct
     8401 taatcttaat ctgatgttga tcattaaatt tgggacgaac atagtgataa ttttctgcaa
     8461 ctactgccaa actctgtaga acatcaacgg ttgcaatcgt ctttgctaac tcttgaagac
     8521 gtttaatata agattctact tgggcacgaa cacgcataaa aatatcatat tctaaatttg
     8581 aagattgctc gcgagcttcg agcatttcac cttcaatttt ggctagttct gctgttccat
     8641 agcgttcaga attttttaat gtcgctttac ggaaaaaatg ctccggtact agtgataaat
     8701 tggaattggt aacatggaag taataaccgt cttttttatt ataatcaatt ttaagagtac
     8761 cgataccact tgctgctctt tccttagctt caatatcagc aatccaacct gtaccttcac
     8821 gcatcactgt tcgataatta tccaattgct tatcaaatcc agacttgata atgtttccct
     8881 cagtgatagt agcctgtgct tctggggcta tcgccgtatt aattaaactt tctaattcag
     8941 gcatagtgtc aattttgttg acaatgatgt caagctcagg ttgattgaag gactgtaaaa
     9001 tcattttaat ccgaggaatt tgtgataagg tctgtccgag ttgcaataga tctttagggt
     9061 tggcttttcc gaaagatact cgacttgcta ggcgttcaat atcatataca ccctttaaac
     9121 tttctgtgag atcgtttctc tcaaaaaaat aatcaagaaa cacttgaata atatcttgac
     9181 gttccttgat tcgattcata cttaccaaag gcctgtcaat ccaagttctc agcattcgag
     9241 ttcccatcgc tgttttagtc tcatctaaca accagtaaag acttccatgc ttcttgcttg
     9301 ttctagcatt ctccagtaaa tctagactat ttttcgttgc atatgacatt tgtaaatagt
     9361 cctttatttc ataatgaact actttctgta aatgactaag ttcacgcttt tgtgttctat
     9421 gcacatattg taaaagtttt tccgcagcag atatttccaa atcagttaac tgctcatcaa
     9481 tcaaatgaat atcattaagt cgttcgtctt cgtatgataa aagcaagttc atctgcttcg
     9541 tcagtaggtg atttttttcg tccgtcaatt gatatcctac tacaatctca cgagctttta
     9601 aatttagtat ttcactacgg acggactcaa aatctgttaa aagggttgcc tgaaactctc
     9661 cagttgagac atccatatat gctagaccaa atgtttgttg atcttgcgaa tcaatcgcta
     9721 ctaagaaatt attagcacta tccggcttcg ttgactccac aaccgttcct ggggtaacaa
     9781 cttgcactac ctcacgcttg accactccca cagctttttt aggatcttcc atctgctcag
     9841 caatggctac tttgtaacct aattcaacta aaacgtcaat atactgttga gctgagtgat
     9901 agggaactcc tgccattggg attggctttt ctgcgttctt atttcgacta gtcaagctaa
     9961 tttccaggat ttgtgctgct tttaccgcat catcataaaa taattcataa aaatcaccca
    10021 ttctaaaaag caaaaaagca tctggataat tctcttttat atccagatac tgttgcattc
    10081 ccggtgatat cgttggcttg gccatttctc actcctaact aacggggctg gctgccatgc
    10141 tcagctcctc aatcattata tttataaacc tactgatagt tctttttcaa aaactttggc
    10201 aatatcttca ttttgacaaa tcaccaacaa ggtgtctgct cctgccattg ttcctaaaat
    10261 ctctggcagc cctttctcat caataaagtt tgctaaaacg ctagcctccc ctaaattagt
    10321 atttaaaact aacataaaac ttgctcgcga tactttcaaa atatatgagc gtaaggctgg
    10381 gctaaatcga tttgaagtag ctgttgataa actataatat agcttacctt ctgggctacg
    10441 taattttaaa agaccaatct cacgcaagtc tcttgataga gtcgcttgcg tgacatcgat
    10501 tccttcattt tttaaatgta atttaatttc ttcttgtgtg ccaatctgcc ctgattggat
    10561 caaacgttta atacgttttt ggcgttctat tttattcatg ttattcaatc ctttttagtt
    10621 agaattatgc ttaccaaatt atatcaaaaa aaattgtctt tttcacttag tagtgatatc
    10681 gtaatcattc tgtaaaaaag ctataaagta ggctagttct ttacagaatg cattatgaat
    10741 ttaatcaaca agttctcgtc tatttttcat aattatgtta aaatagggtg actatattac
    10801 taattaaaat aggagatacc atggatacaa aacatctaat tgcgagtgaa attcaaaaag
    10861 ttgttccaga tatggaacaa tcgaccattc tttctttatt agaaacccca aaaaattcga
    10921 gcatgggaga tttagctttc ccagcattct ctttggctaa aactctacgc aaagcacctc
    10981 aaataattgc tagtgacatt gctgaacaaa ttaaaagcga ccaatttgaa aaggtggaag
    11041 ctgttggacc ttacgttaac tttttccttg ataaagccgc aatctcaagt caagttttaa
    11101 aacaagtctt atctgatggt tctgcctatg ctactcaaaa tattggtgaa ggacgaaatg
    11161 ttgccattga catgtctagt ccaaatattg ctaagccatt ctcaattgga caccttcgtt
    11221 caacagttat tggtgatagt ttagctaata tttttgataa aattggctat catcctgtta
    11281 aaattaatca ccttggcgac tggggtaaac aatttggaat gttaatcgtt gcctataaaa
    11341 aatggggcaa tgaagaggct gtccgtgctc atcccatcga tgaactttta aaactttatg
    11401 tccgtattaa tgctgaagcc gagacagacc ctagcgttga tgaagaggca cgtgagtggt
    11461 ttcgtaaact tgaagctaac gatcctgaag caactgaatt atggcaatgg ttccgtgacg
    11521 agtcattatt agaattcaac cgtctttacg atcaaatgaa tgttacattt gatagctata
    11581 acggcgaggc attctacaac gacaaaatgg atgaagtgct tgaactgcta gaatctaaaa
    11641 acctcctagt cgaatctaaa ggtgcacaag ttgttaacct agaaaaatac ggtattgaac
    11701 atcctgccct cattaaaaaa tccgatggag caactctcta cattacacgt gacttagctg
    11761 ctgcacttta ccgtaaacgt acctacgatt ttgcaaaatc aatctacgtt gtaggaaacg
    11821 aacagtctgc acactttaag caactaaaag ctgtgcttaa agaaatggac tacgattggt
    11881 ccgatgatat gactcatgtt ccttttggcc ttgtaacaaa aggaggagca aaattatcaa
    11941 ctcgtaaagg aaatgtaatt ctacttgaac ctactgttgc agaagctatt aatcgtgcgg
    12001 catctcagat tgaagctaaa aaccctaacc tagcagataa agataaagtt gctcaagcag
    12061 ttggtgtagg tgctattaaa ttttacgatt tgaaaactga tcgtacaaat ggttatgact
    12121 ttgacttaga agcaatggta tcatttgaag gtgaaactgg gccatatgta caatatgcac
    12181 atgctcgtat tcaatcaatc ttacgtaaag ctaactttag cccatctaat agcgataatt
    12241 acagtctcaa cgatgttgaa agctgggaaa tcatcaaact catccaagat tttcctcgta
    12301 ctattgtacg tgcggcagat aattttgagc cctctattat tgcaaaattc gctattaatt
    12361 tagctcagtg cttcaataaa tattacgccc acacacgtat tttagatgaa gacgctgaaa
    12421 tcagtagtcg tctagcactt tgctatgcaa ctgctacagt attaaaagaa tctcttcgcc
    12481 tcctaggagt tgatgctccg aatgaaatgt aagaaatgac actactaaga ctgtcaaatt
    12541 tatgacagtc ttaatttttt atattataat aatagcacaa caagttacag gagaatgcca
    12601 tgccatcaga aaaagaaata ttagatgctc ttagtaaagt ttatagcgaa gaagtaatac
    12661 aagcagatga ttattttcgt caagctattt tcgagctcgc tagtcaactt gaaaaagagg
    12721 ggatgaactc actcctagct acaaaaattg actcacttat caatcaatac gtcttaacac
    12781 atcaatttga tgctcctaaa agcatatttg atcttagtcg cttggtaaaa acaaaagcta
    12841 gccattacaa gggaactgct atctcagcta ttatgcttgg tagtttctta agtggaggac
    12901 caaaataaaa atacaggcca gacagcctgt atttttattt atcgtaattt cttcgtcgaa
    12961 ttttaaaatc tgagctaata acttcatcta catttataac agaaataaaa gcatctgggt
    13021 ctaaatcagc catgatttct ttcacatcac gcacttcact tgggttaaga gctacgtaca
    13081 agacatcatg ctctctacct gagtaggcac cttggccccg taaaaaagtt acacccctat
    13141 taatttcatg caaaatggtt gctgctgcag attctgattc tttggtaata atgatcattc
    13201 cacgaacagt gtagccccca ttttcaacat ttgttaacac ttgactaaat acaaagctag
    13261 ctactaacgt atatagcatc tgttgtaaat taatataaac aagtgaagac gttagaacta
    13321 tagcatcaat aaacaataag gtttgcccta attttattcc agatttttcc tctacaatac
    13381 gaccaataat atctgcacca cctgtagttg caccatatct aaatactaaa ccactaccag
    13441 ttcctgctaa aattccagca gctactgcaa ctaacatcat atcgtttttc aaagggactg
    13501 ttacagggat ttgttgccaa aaccacatga aaaaagacat taacactgta ccatatatag
    13561 ttaacaataa ggattttttc cctaaaatcc tagcccctaa tataaataac gggatattaa
    13621 ggagaagtga tgaaagtgct ggattaactc cgaaaagtgc atggataatc aaagtgacac
    13681 cggaaatacc tccttcagcc aaatgattag ccatattaaa tttgacaaag ccgaaagtat
    13741 agagagccac accaaaggca attaaaatag tttcttttat ctttgttttt aaatccagtt
    13801 tcaacatagg cttaatcctt ccttattcaa aaaagctaaa gttgtttaaa caacttagct
    13861 taataacatc ataatgtttc cttagtggtg gctgaccaaa agtcatatat acagttaatc
    13921 atcatcaacg aatcgtccaa taatatgaac attttctgca acggaaacaa aggcttttgg
    13981 atccgctttt agcattaaat atttaaaatc actaaattct tcacgtgtta atatagttat
    14041 taaaactgct tttttctcat gattataagt accttcagca tcattgatac aggttacacc
    14101 acgatgtaaa tcacgatgga tacgtttaat gacgcaatca ggcttactgg taacaatcat
    14161 cgcttgcatc tttttttgct ttgtaaaaat ggcatctgta actctacttg atacaaaaat
    14221 ggtcaccatc gagtacaaag cgtacttcca accaaataaa agacctgcaa ataataaaat
    14281 aataccatta ataataaaag aaatactacc tacatctctg cctgttttct ttctaattgt
    14341 caaactgata atatctgttc ccccactaga aattctagac ttaaagctaa aaccaacacc
    14401 agctcccata attaaaccac caaagatagc attgattaag ggatcagttg taagggtgat
    14461 ttgaggcatt agttgaatga agatagagct tacagttact gtgataaacg taaaaatagt
    14521 aaatttatgt ccaatcttac gccaagataa tatcagcaaa gggatattaa tggcataaaa
    14581 agctaaagca actggtattt caaaagaaaa ccagtgttta ctgacagctg aaataacctg
    14641 agctaaacca gttgctccac ttgaataaac atgtccaggt tgaaaaaaga aattgacagc
    14701 aacactggat agaataccat acagcaatga agccgaaatt ttctctgcat atttttctcg
    14761 tgagatactt tttaaagtgt gtaagagacc aaatttttta gcccatacac tgataatata
    14821 ttttaccttt ttttctaaag gggttcgtct agttttcata actttctaca gtaagtgata
    14881 attcttctaa ttgttgttct gaaacgagac taggagcttg agtcattgga tcactagcct
    14941 tattgttttt agggaatgca ataacttctc gaatattgtc cttgcctgct aacaacataa
    15001 caaagcggtc aaggcctatt gccaatccac catgtggtgg gaagccgtaa tccatagctt
    15061 caagtaagaa accaaactgt tcctgagctg attcagcgct aaatcctaaa gccttgaaca
    15121 tacgctcttg tgtatctttt tggttaatac gtaggctacc accgcctaac tcatatccat
    15181 tcaaaacaat atcataggca actgcacgta ctttagccaa atccccttcc aactcatgcg
    15241 ctgtctccgc tgttggcaag gtgaatggat gatgagccga catatatcgt ccctcttctt
    15301 ctgaccactc aaacattggc caatcaacaa cccataggaa attaaattta gaataatcaa
    15361 tgagttcaag ttcttttgca atgcgtgtac gaagcgctcc taaagtctcg ttagctactt
    15421 caagactatc tgctacaaat agaattaagt cattattctc tagctgtaaa gcttctgtca
    15481 acctaccttc aatagctgtc aagaacttag caaccggacc tgaaatagta ttatctgcat
    15541 attttagcca tgctaaacct ttggcaccgt attgttttgc aatttctgtt aatttatcaa
    15601 tattttttcg agagtacttg tccgccttat ctttgacaac aatcgcttta actactgaag
    15661 cttctgagaa caccttgaag tcaacttctt tgacaatttc tgtcaagtct tgtaaaagca
    15721 tatcaaacct tgtatcaggc ttatcagacc cgtagttatt cattgcatca tcatatgcca
    15781 tccttgggaa tggtaaagaa acctctaaac ctttagtgtc tttcattact ttagcaatca
    15841 taccctcaac aatgtcttga atttcttgat cactaaggaa tgatgtctct aagtcaactt
    15901 gtgtaaactc aggttgacgg tctccacgta aatcctcatc acggaaacat ttgacaattt
    15961 gataataacg atcaaatccc gcattcatca atagctgttt tgtaatttga gggctttgtg
    16021 gaagggcata aaaatgtcct tggttgacac gtgatggaac caaatagtct cgtgccccct
    16081 ctggagttga ttttgttagc atcggagttt cgacatcaat aaactctaag ttgtcaaggt
    16141 aattacggat tgagtgtgtt actttcgcac gtaatttgaa attttcaagc atttccggac
    16201 gacgaagatc caagtaacgg taacgcatgc gtgtatcatc atttgcctca ataccatctt
    16261 taatttcaaa tggtgttgtt ttcgaggtat taagaatcga taattcttgc acttttaatt
    16321 caacttctcc tgttggtaaa ttatcattag cttgttctcg tgctgtcact acaccgctaa
    16381 cctctataac aaattcacta cggagacttt ctgcggttgc cataactgag gctgatactt
    16441 cttcaggatt aataactaat tgcatgattc cctcgcgatc gcgaagatca ataaaaataa
    16501 ggcctccaag gtcacgacga cgaccaaccc atccttttaa agtaatcgat gttcctatgt
    16561 gctcactacg aacacgccca gcatacatac tacgtttcat ctgttttctc catttaaaat
    16621 attcattcca acctattata acaaaaagca cctaaaaacc ccacccatta aggagagttt
    16681 ttaggaactg ttttacaatc ctaattgttt aagcacagat gaaaaatctg ttttaatgtt
    16741 ttcaaaacta actttaactt cttgacgtgt ttgattattt tttaaaccga cctctttcga
    16801 atctacttca ctgctaccta atgtcataat cactttagca ttaaaagtat ctgcagactt
    16861 aaattgtgct ttgattttac gacccaaata atcacgctct actttaaaac cctgatgacg
    16921 tattgattgt gccaaatcta aggcagctag attagcctct gatcctaata cagctatata
    16981 cagatctata gtattttcta ttggtaaaga aatcccttgc ttatctaata ttaagagcaa
    17041 gcgttctaat cctaatccaa aaccaaatgc tggtgtttct ggtccaccaa aataggatac
    17101 taagccgtca taacggcctc cagcacagat cgttaattca ttatcttcaa tctcagtgat
    17161 aaactcaaaa atagtatgac tataataatc aagaccacga accatattag tatcaatgat
    17221 gtatggaata tttagagcat caagcatatg acacacagca tcaaagtggg cttggcttga
    17281 ctcatctaaa taatctaaaa tagaaggagc attttcaact gctagtttgt cttctggctc
    17341 cttagagtct agtacgcgca atggattttc attcaaacga cgttgactat ccttagacaa
    17401 ttgttcacgt atgggtgtta agtaatcaat aagagcttgg cgataggcct gacgactttc
    17461 tggattccct aaactattaa gatgaagttt aacgtttttg atacctaaat cttcaaatag
    17521 atgatgtccc attgcgattg tttcaacatc agttgctgga ttatttgatc caaagcactc
    17581 aacgcctact tgatgaaatt ctcgtaaacg accagcctgt gggcgctcat atcggaacat
    17641 tgatccaata taatacattt tggttggttt ttgaacttct ggtgcaaata gtttattttc
    17701 aacataagaa cgtacaacag gtgctgttcc ttctggacga agcgtaatgt gacgatctcc
    17761 tttatcatga aaatcataca tctcttttgt aacgatatcc gttgtatccc caactgagcg
    17821 actaataacc tcgtaatgtt caaacattgg tgtacgaatt tcatcataat gatattgttt
    17881 aaagagattg cgtataacat tttcaacgta ttgccacttt gctgattctc caggtaagat
    17941 atcctgagtc ccttttggtt tttgaagttt cataagttta cctcttcgat tagttttctc
    18001 tttaaacatt ctaccatttt ttcttatatt ttaaacaagc atatatcatt tgctttagtt
    18061 tgatagtatt ttcaccgtga aattgtaaaa ataaaaagtt ttgtcaagta actttacttt
    18121 acaaaaaaga tatgttatca tagtgaagtt gatgaaaatc aaaacttaca catcgaaagg
    18181 aagaataaga aaatggcagt acctgcacgt cacacttcaa aagcgaagaa aaacaaacgt
    18241 cgtacacact acaaattgac agctccatct gtacaattcg acgaaactac tggagattac
    18301 tcacgttcac accgtgtatc actcaaagga tactacaaag gacgtaaaat cgctaaagct
    18361 aacgaagcta aataatagaa gggagatacc atgcgcgtaa atattacact tgaacataaa
    18421 gaatctggtg aacgcttgta ccttacttca aaaaacaaac gtaacactcc agaccgtctt
    18481 caattgaaaa aatactcacc aaaacttcgt aaacacgttg ttttcactga agttaaataa
    18541 gacatcatac agaaagccta tgaaatcaac gtttctaggc ttttttgctt gcttaaaatg
    18601 tggttttgtc tgatttttgt ctgattaaaa gtatattatc tattcaaaca tctcctatat
    18661 ccgtcatact tccaagtttt tatggaatca ctttaaagca acttttttcc acttgcgaat
    18721 cttagttcta aatgttccga aaggagctac cgtattaata tgaataaact tataaacttc
    18781 ccagactgca ttcttagtcg cactatcagc ccaatttctc atatgaggtt taaataggac
    18841 ttcgtcgctc attgaatcaa tcatgttacc tatagcatct acattatcat tgagttgagc
    18901 cgtaagttct ttgatggtta gactagcgta ggtctcagta aaatgctgat atagctctcc
    18961 aagttgattc catttaaacg tctcagttgg tgttttaact tctaagccac tctgttcatc
    19021 tgactcccat tttagtatca gggtagtcca gccaacctga taggctagat tctccgctgg
    19081 tgtcctatca acctcatcaa ttcttaaatc tttcaacttc tcaggaatgt cattgaattc
    19141 agcaatatat tttttatatg attttagtac ttcttcctta agttcgtttt tatctgaata
    19201 tgttctcatt tctttcctct ttaatttagt caagttcatt gtatcatatt cttttctctt
    19261 tttcttagga agtacagctt ttataattct atatatcaaa tgaattttat tcaatcaact
    19321 tttctagctt ttcaactgcc cctctcatgg tatctttttg cttcttaatc acatggagat
    19381 agacacgctc aatctccttg gaatcctccg agtgtccgac aaattctcga atagtaggga
    19441 ggtcaatacc ttcgctagcc ataatagaga taaagctatg gcggaacata tgagggatga
    19501 catgaatcgg tctgccaaag tctagaaagc tatcgatatc cttatagggc ttgcctgctt
    19561 tcttgttata ctttgctgac tgactagagc cacctctcag gaattgtgag agttcgttgt
    19621 gataaagcgg agagccttgt cgattacctg ccctaatata ggttctagta aagagatacc
    19681 ctaagtcttg gtaagtcgga ctgaattgag cttgaaattt atttctgtca atatagtgac
    19741 gtagaatctt ctcaacctca gggctcattg gaatcgtgcg gacactttcc ttggtcttaa
    19801 ggtctgctcg gtagcgttcc ttttcgtcaa gattgagtgc ttccatcata tcatccgatt
    19861 tattcttact tgccctctgc caatgaactt ttatccactt tttctcaaaa tcaagcatgt
    19921 cttcatttaa gcctaaaacc tcacttggtc gcatgcctgt tagaaaaatc attctcaata
    19981 catctgcgat aagttggtta tcaggtcgat tagtccagct ctctatcagg cggagaacat
    20041 ggttaacctc acttgcttct agatacttct ctgctatatc ttcacgcttc ttgtcctgct
    20101 cctcactctt tgtcagaact cgccttttaa ccttattgct ggcaatcggg tcttgtgtaa
    20161 gagttatctg tccattttca actgaccagt caaaaatcat cttaaggtag atatgcagat
    20221 ttctcgccat gtgcatagaa gaagcattga taagattttg aatttcatct gtcataaagg
    20281 caaatttcgt gatggacatt ctggtatcaa agttcggctc aatatagcga ttatagatag
    20341 taacccttgt tttaagggta ttattggctg gcttgaccct ttcatgccat gctttaaagg
    20401 ctttaccgta aaaatttcca tctttatcaa atggcttggc gatttcttct agggttaaaa
    20461 gtttcttggc tttcttaggc ttaatgccca ttagctcggc gatatagtca tcaactaatt
    20521 tattcagttc ttgcgtgacc tgagtaatta attgtgtgtt tttaatatcg ctgaccaaga
    20581 gacgttcttt cccagaaggt aactttttgc tcggtgttgc cttggtggta aaccaccctt
    20641 tactagcacc tgtagaaagg tatttcttat gagatttttt agtaataggg tgggtgaatc
    20701 gcatagcgaa tttcaccaag acattacccg ccttataagg gtctggtcgc caagaactga
    20761 tgataagtac tcttgaacca ttatctctca tagggactac ctacgccatc tttgcgacat
    20821 actgattggc aattttgtag tcaaaccacg ccacaaagac ttctgagtgg ataaagacat
    20881 tgccgtgtgt tcctttgata ccgaaagcgt aagggctgtc tttcatctga cgaatgtaac
    20941 tattaagtgt tgcaacggtc atgccatgcc agagctcatc tagaaagacg gatttgcggt
    21001 accagttacg ggttaagtca aaatcatgtt ctgtttttaa tgttgccatt gaattctcct
    21061 tctgttttag ttataatcaa gttagttatt tttggaaagg cgatttgtag cttgctacag
    21121 gtcgtctttt cttatgcccc aataggctga gcttagttgc tcttgtttgt actgctgaat
    21181 gtcagtctcg tctgtttcgg ttagcttaga aatccccaat tcaagtaagt gctggatata
    21241 gctatctgcc ttttcttgcc cgtgagattc cgccttagcg acatagagtt ttccgagtgt
    21301 tcgtgagact tgtttctctg tccacttctc agaacgtttt agggtaggtt ttgtccgttt
    21361 tacgcttaag accgtctttt cttgtgtctc tagatagtca ttccaccatt ttaccgtctt
    21421 tcttcgccaa cggttagaat ccttgtccct cgggctgaca aaacggtagt gtccgttaat
    21481 aacttcaaag taaatctctt tgagcggtcg cccctcttta atctgtttgg ctagtaaatt
    21541 tgctttttcc tgccagcacc tcagctctgc tcttgtccat gataagggcg tatttggcaa
    21601 ttcttggtct agcttttgtt ctaacaattt gttatagaca caccattgct gagtacctct
    21661 aacaccaatc gtaaccattt caccgacctt ttcaccattt tcaagaaggc tcttttcatg
    21721 gtaatcaaag gttttagcgg ttgatatgca gagttgcttt ttacaataac tgtaaatgag
    21781 tgggacggat agactgtcat caaagatatc gttggcaata tctagtctcg taacgttact
    21841 atgacattca gaaatgcgtt tcatcaaggt tacccagtta ttgacattgt tttccatata
    21901 ttcttcatac tgacggcacc cctgccctct cagttcgata aagactccca tctttgattg
    21961 ccagtcctta ttgaagtaaa cctttatctc tgagaaggaa taatgccgtt gatacttgtt
    22021 tatcccccat tcattgagga caaacttgtc tttaggaaga atgaggattt tctcaatgac
    22081 atcatctgga gccaagcctt taatcgttac ggctaaatag tctaacatca ctgagttatc
    22141 catgtctagg ctccaataaa tttaatatcg gttgccacta gctttaagcc accccaattc
    22201 cctccaccat tattgttggc tcgccaacca agatggactt ctggattgac aagttctacc
    22261 acaagggagt ttaacccatc cttttcaacg gatttagcca acttgtcaag attgtctaca
    22321 atctccaagc gaatgccctt aaggtcatca gaagaatagc ccaagtcttt caaggcttga
    22381 attttgtctg tatcttgtac agagcaagtg attttagcca cttcatctgt cgcaatgctt
    22441 tcgccattga catttttata gactggtgca acccgtgaag aaaggatagt cactttcttt
    22501 ggaatgtcaa attgtgattt atctaatttc aaatcaccaa cttttatggt ttcgtctgtt
    22561 aagttaaatt ttgtcatgtg tttttctcct gttcataaaa gctacgccta catgaaatct
    22621 gtcataaatc gttgatatca tgggctttat aaagattttt ttcgtgaaat ttgcttcaaa
    22681 tcgctgaacc ccttgcttat caagggtttt gaccacattt cgtgtatgtg tgcaccccct
    22741 aatagggaac ggggtgcaat ttcaatttcg atttcagccg tttgccacct tccccacctg
    22801 ctctgggcta ccgtattgac cactcgccca gcggtggggt ggcgaaccgt cttcatcgtt
    22861 tgaagaaacc aattctctta gatgttttat cccctgtctc tttctctaag ttctgaattc
    22921 tgacaagaag cacttctaac ttctgtttca actcagttag gtttctttgg taatcctctt
    22981 gaatttccat aaaagaaact tctagctcgt ctacaagctc tctaatgggt ttctgatggt
    23041 cttttccctt ggaactccca taaacgctta gaattgccct ctcaagccct aatgaggact
    23101 tcttatccgc gaccgcttga aagtaggcaa ggtcatcatc tgtaaagaga tagattttag
    23161 aataagattt ccgacccgtt ctcactctac tttctttaaa ttgagtacca gtcaagcttt
    23221 caatttttaa tcgccacttt ttcagtgttg aaactgagtt aagaccgtca accctatcca
    23281 caacttcttg ataatcatat tcattcatta gcttacctgt gttttctgat tgagaaagtc
    23341 ggtcacaatc tgcaaggctc tagattcaat caatcgttca aatcccgtac tcttgataag
    23401 gtgatagagt tctctgtcat ctaaatcttt gcagtcctct ggtttgatga aaactttctc
    23461 ttgtgtctct ggcgagtagg caacccactc caccatttgt agttcaatac ttgtttgatt
    23521 cataattacc ctcttttctg acgtttgcat ccaactagca tttatccgaa atatctggta
    23581 taattacagt aattagattt actgtccttg aacgatgtac cagattgttc aaggattttt
    23641 tctaccttcc tttctttttt gattgttaag ttagctcctt tctatcaata atatacgatt
    23701 ttatatacga ttgctgtaaa ttttttgaac aatcctaatg ctcattaaaa attatatacg
    23761 aaaacatata cgatgtcaag tattaaccac aaaaaaatgt acgattttat aaactactat
    23821 gttataatgg tctgagaggt aacttatgtt tgaaatactt aaacctgata aaaaggctgt
    23881 tgggaaaaga ttgaggcaag tcaaggatga gttaaatttg tcttttacag attttggaaa
    23941 tcgtcttgga ctaaaaaaat caaccatcaa cgcatatgta agaggagata acttagcgcc
    24001 tcttgaagtt ttagaaaaag tatcaaaaat ctatggaaaa cctgtaggtt ggttttacta
    24061 tggagaattg gaagaatata ttgaacttta ccttagaaaa cttggttatg gtacattcct
    24121 agatgaatat cctgagacac cgttaaagat aaaggatgag ctttttagga ttaaagcaaa
    24181 tccaaaacaa ttaatcacaa ctaacggggg agtaaccatt ggaacatttg agaatcctaa
    24241 ttgggaaaat gactttggtt acccaaatga aggctaccta gatgatatct tttttgaaat
    24301 gctttgtgaa actctaaaga actcaattag acaggaagct caaacctacc taagtcagca
    24361 agaaggactt gacgatacta aaatcaaaga actctctaat tactttgcca accaaatcta
    24421 catctcatat gattttgtcg gaaatttaga aaatctttct aaagataaaa tcaagcaaga
    24481 agttaaagaa agctatgaac gtctgcttaa agattcacct caagcaggtg tagaagttag
    24541 ctatgattcc aattacctac ttggttttct aataaataca cttgcggata gtcaaacaac
    24601 aaggcaatta ctttcagata tgtcacaagc aacagtcggt atgccttttt cacaatttca
    24661 tgagggacac gaattagttg aggttatcca atctgtcaga ccacggctga tagaacttta
    24721 caataacacg acctttgatg attggactga ctgggcggag aaatgatttt agaaaggaag
    24781 atgcaaatgg ataacaaatt caaaaatatc ccaaaacggc atttaccaca aaaactccca
    24841 tttgactaca cgtcaatata tactgaccca caaatcatta aattaactac tagggcaaat
    24901 attgcaatcg gtacttacga aggatttcta gagtctatca ttaaccctat gctactaatc
    24961 tcccctttgc ttagccaaga agccgttctc tcttcaaaac ttgaagggac ccatgccacc
    25021 ttgaaagact tacttaatta tgaagctggt aataaagtag atattgagcg tgatgaattg
    25081 catgaaatca ttaactaccg aaaggctctc ttttatgctc tagaaaacat ttccaccatc
    25141 aacaacatcg attctaaagg attaccgtta tccaatagaa ttattaaaga aatgcacaaa
    25201 attcttctag ataatgttag aggtagtagt aaaaatcctg gtaattttaa gagaagtcaa
    25261 aactatatcg gtagtgtatc ctcgatttca tacactcctg tccccgcgga aaaaacacca
    25321 gaatacatgt ctaatttgga gcaatatatc cattatgatg acttagactt attggttcag
    25381 tcagcaatta tccatgctca atttgaaatg attcaccctt ttgaagacgg taatggacga
    25441 attggacgat tgcttatacc actattcctg tactatcagg aactactttc ttatccaaca
    25501 ttttatatga gttcgtattt tgaaagagat aggtctcttt acatatcaca cctatcaaac
    25561 atttcaaaag acaacaactg gaaagactgg tttgagtatt acctagaagg agtgatattg
    25621 tccgcagaag agagtacaaa aaaagctcag gacatactaa gcctatataa cattatgaag
    25681 gaacaagtca tccctaaact taattctgtt tcaggtatcc agcttcttga ctttatattc
    25741 tcggctccaa tctttaaggc agaacaagtt agtgaacacc taaaaatttc taagagaact
    25801 acctatactt tactaaataa gttaattgat gaaggttatc tttcaacaga taatgctcaa
    25861 aggaatagaa cctactattg tcctcaactt ctttcaatcg ttcaataagg tatctcttaa
    25921 tgaagtaatg ttcacctctg cttcacttat ttctttttta gtgaagcaaa agtcatcttt
    25981 acttctctta tctatttctt aatgaagcga aacctgtttt ggcttcactt gttttttatt
    26041 atgtgaagca gagttggatt tcgcttcact tagcgactgg taagtgactt atcatttata
    26101 ctttattttt atactgaata aaaggaattt tacccatgcc caagaatagg ataaacaatt
    26161 tcatcaatat aggaggctta atcttagtaa tctcttttct actattagtc cttgatatag
    26221 ttaatcttcc aacgaaattt ggttttaata taaagacttt aaatattgaa atttggaata
    26281 tttacgctac gcttttagga agcgggttaa ctctgtatgg tgtaattata acaattaaca
    26341 gtcagtatga gtcacagata gaggatacta aacggattgt aaagcctatg attaatgtgt
    26401 cacctgttga cgagtatagc tacaaggata agtatattca atttgacttt atattcccca
    26461 aagcatatcc aaacctttta agaaaggata atattgacac tccaaatgtc accatctctc
    26521 tggaaaatct tggaactaga gaattgtata atttatatat aggaaatttt gaagctactt
    26581 attttgatga tggtggctat tattatcaac ttaatccaat tttatattca aaccataagg
    26641 tacaacttaa tcttatgttc tatgaaaaaa atggttatct taaagaattt aatcaggaag
    26701 ataaatttca tttgctcatt tctcctatca aattttcaat ctattttcag gattgcttga
    26761 ataattggta cgaacaatcg tttaaattac gtattttcca ccaagttcaa gcagacaggt
    26821 cagatgaaga aaattcacta acagttagta ttgaaagagt agagatatat tctgccccta
    26881 aagaaatttc tcccgattca cttccgtgga taactaatcc aagtaagtta tgccagtttg
    26941 ggtgaatcac taataaagcg accacactct tatccaattt attagcagtg aactaaattt
    27001 tgtctgattt ttgtctgatt aaaactgatg caagggcaaa atcactctat ttcatcaata
    27061 attgaaagat ttaaaagccc gtaaaatcaa cgtttctgaa cgtaacaaat attgctaaat
    27121 cacacattac gcttactgaa gttaagtaag ggtttcagta cacgtcaata gacgtcaaaa
    27181 tattgatttt aagcgatttt tgattttttg aaactcatta tatcgcacta caaatcaata
    27241 aaaactctac cttttactct acctttttta gataaatata tcatctggat attgaagtta
    27301 agccctgcta tcatttcgat ggcagggctt tttaatattt gtacactaac ttcttatcta
    27361 tattttggtg gattttaagt tgacatctac aaagtttaat gttagaatac attcaaaaat
    27421 atatttgaat gaggtatttt atgattcaaa atgttgttac ttcaataatc ctgtattctg
    27481 ggacagccgt agacttactt attatcctaa tgttattttt tgccaaaaga aaaagcagaa
    27541 aagacatcat taacatctat ttaggacaat ttctaggctc tgttagtcta atattcctaa
    27601 gtttgctttt tgcatttgtc ttaaattata ttcctagtaa agagatttta ggtttactcg
    27661 gtttgattcc aattttccta ggcctaaaag ttttgctttt aggagattct gatggagaag
    27721 ctattgcaaa agatggtttg cgaaaagaca ataaaaacct gatttttcta gtcgctatga
    27781 ttacttttgc aagttgtggc gctgacaata ttggtgtctt tgtcccatat tttaccacct
    27841 taaatttagc gaatttgata gtgactttac ttacttttct agtcatgatt tatctcttgg
    27901 ttttttctgc acaaaaatta gcacaagtcc cttctgttgg agaaactttg gaaaaatata
    27961 gcagatggtt tattgccgtt gtctatttag gattggggat gtatatcctg attgaaaaca
    28021 acagctttga catgctatgg gctgtgttag gctaggagaa aaaattatga aaaaagatag
    28081 tatctgtcaa gtggatgtta taaatcaaca aaatgttaca accgcaacga actaccttga
    28141 aaaggaaaaa gtccaaaaat cacttcgcat tttatcaaaa tttaccgata ataaacagat
    28201 aaatatcatc ttttatctcc ttgccgtcga agaactctgt gtctgcgata tagcctgttt
    28261 attaaatctc agtatggcat ctgcctccca ccatcttcgt aaactagcca atcaaaacat
    28321 cttggacact agaagagagg ggaaaattat atattatttt ataaaagatg aggaaatcag
    28381 agattttttt aatcaactag gataacaact atttctacta ctttctactt ttccatgatt
    28441 atagggggat tatatatgaa attttttgaa gaaaattatt cacaagaaat acctactcgt
    28501 atcaaaaatt taagaaaaaa atacaatatt acacaaagcg aactaggcaa tgcaggtcaa
    28561 gttagtcaag ttgaaagtgg taagcgacca attacgagtt cgatgttagt ttatttaaat
    28621 gctctaaccg cttcgagcta tacgtatatc gtttttggag agttagatga gtttattgag
    28681 aatctgtttc attatttttt cagttctatt ttatatagag atttagaggc tgtagatgaa
    28741 aaactatatt ctttcatgag tgatgattta atttcaatcc aatcaagttg tctaagtatt
    28801 gccaagacat ttgcaaattt caatattcaa agaaaaagat ttatgatttc tactgaaact
    28861 gaaatggata cttttcacaa gaaagatgac attgacgtat gggttggtgg aaaaagtgat
    28921 aaccccgcta gaagttttag aacccgtacc atcaatgaat tgactgtcat tgattttgaa
    28981 gaaatgtttg atattctttg gttgatgtta ggggataatt taattaaatc atttgaagtt
    29041 aatgtctgtg gtatcctatt tgaattaggc ggaaatgaca taccctcaac atttaggcaa
    29101 gaaaacattg atcctcttat caataaatgg tggtatgata atgtttcgac agaaattatc
    29161 cctaatctaa taaaaaaact caaagaaaat cccttgttta atatcggatt tatggtaaat
    29221 gatatattag aaagaatgta taaagagaat attccaaaat cttaccttac atccgtccca
    29281 ttagttattt ctcaaaaagg aagaacaacc tattcgttta gtatgactgg tggtcaacaa
    29341 atagatggag tgaaattcac acagatatat gaggactata tgaaattact cagtcaaggt
    29401 aaggatatcg cagagttata tcaaaaatat tctaaagaag agttggcaaa tctaggcatt
    29461 aatatttatc aatccaatga tatagaaagg actgaggaaa gaacttttga tgaaattatc
    29521 agttgggttt ccaaccctta tgcaacaaga ccaattcaag aaaggcacac tattcaatta
    29581 gagccaacaa gattttcact agaggataag aaaagaattg aagaagctgc agctcaagga
    29641 ctaagcgaaa tcgaccttat tgatttagtt gacctatatg atattaattt agacaataca
    29701 agcgtcaatc gccatattgt ggggttattg actaataaca cccaagtaac atactatttc
    29761 caagaacaat taaataagga gttgctgtca atggctcacg ctttagataa cgtacaacag
    29821 gcctttatta aattattaag tgaagaggag atacgaaaat ttgctcttta aagactaggt
    29881 gaatcgccta gtcttttttt gcgcctcgtt ttacaaatta gtctattgaa gtaaaaaaag
    29941 aagtaaaaaa taaaagaaca ttacgcctta tactgaatat atcaagaaaa actcttgata
    30001 taaattttaa tagtccaaat tgatggccat caaaggacag attgaaacaa atatgatgac
    30061 atgacgataa aggtcatgtc ttgcatagaa ttgtttcgct gtttctttgt tgtgcctttt
    30121 ggtatactca gcgttcaata tttgctcatt ttgtttcctt ctgtccaaag gacagaaagg
    30181 agctcatcat tatgagtaat aaagtacaag aacgtcgtga gcgtaaaatc aaagaagcta
    30241 ttaaagctaa aaattgggat gaagtaactc gtttgcttca acaagaacaa agtaacgcag
    30301 aacgtcgtga ccgataccac aatagacgaa tcaaagacga aacaatagct agcaaaaatg
    30361 ccaaaaagtc tgtacgttat gatgtcattg caagttctga tttgaatccc gaagaagctt
    30421 taatcctaga ggagttaaga caagctattc gtgaagctaa agcatcccta tcagaaattg
    30481 acagtaagat tgttgaaatg atagcggaac aaggttcaag ctataaagaa acggctcgct
    30541 atatcactga acattataag aaaatgagtg atgtaactgt taaatcccat tattgtaaag
    30601 cgctaaaaaa attagctcct ttattaaaag cttaccgcta atccctcaaa tccagtgtct
    30661 ctgtcactgg attttttgta aaaaaaaatt aaaaattcaa caaataactt tgcgtttagc
    30721 tgtctcattc tccttagtaa tgaggaggtg gtcagataac ctaaaaaaca aagtccagct
    30781 catgacccaa accgagcctg tcgcattcac actatctctt attcataagg aggatttgcc
    30841 ctatggcgtt taaaatgaaa accacaaaag gtggttatac aactacattg gctgataaaa
    30901 ttatcagtca aaaacaaccg atttactcac ttagtaccga acttgaacca caacaacggt
    30961 ttgaagaagg aaaaccaacc ggagaaatcg tagcttataa agcatggttt gtacaggaag
    31021 ggcaagaccc tttccaagta aaatttgaag aaacgattga gcttccagct tttcaatcca
    31081 tgattcaatt tgacacacta caagcctgcg aagtaaaata taacatctac ttcaaagcaa
    31141 atggtatcaa ggaggttcgg taatgacttc acaatctcgt gatgtgctga accaatctta
    31201 cctgcaatct tacctgctcc agagcctaaa catggctctg ggggctttaa tgcagggaga
    31261 aaccagctac accaattctt ttaatattgt tattcaagca gacggcttta tctttgtccc
    31321 tcgtctacca tgtgcttata tccttgatga tgacttgtac aagaaaatct ttctgattgc
    31381 caatgcttca ctttatccac aatatacgct tctaaaacaa aacgctacct attttgtccc
    31441 tctagaaaca gatgacttac acactcagcg tggattattt ttcccttgga aaaagggaat
    31501 ttcagaacgt ttagtcattc cagatttgga taagttttca gctagcttgc cacaggggaa
    31561 aatccctatc atgaagtact ttgaactcaa tttagataag gttaatcact gggctattgc
    31621 tggaaattca ggttctggga aatcgtacgc tctcacttat ttcttgagtg tactaaagca
    31681 tatgtctgac ttgattatca ttgaccctaa atttgatacc ccaagtcgat gggcaagaga
    31741 aaatcacgtt gctgttatac accctgttga aaaccgttca aaatcagatt ttgtctcaca
    31801 ggtcaatgaa caattaagtc agtgtgcaac tctcattcag aaacgacaag ctgtcttgta
    31861 tgataatccc aatcatcaat ttacccatct aactattgtt attgatgaag tcctcgctct
    31921 atcagaggga gtcaataaga atatcaaaga agcctttttc tccctactct cacagatttc
    31981 cttattagga cgtgctacca aaatccatct gtttttggta agccagcgtt ttgaccacaa
    32041 tactattcca atttcagtga gggaacagct aaacgtattg ttgcaaattg gaaatatcaa
    32101 tcagaaaact actcaatttt tatttccaga cctagaccca gagggtattg ttattccaat
    32161 aggacatggg acaggtatca ttcaagttat cgataatgaa catccctacc aagttctgcc
    32221 cttactctgt ccaacctact acactaaacg aggtattcta tgaccaataa aacctacttt
    32281 atgagaagtt ttaatctcat actatattta tttctatttg cacctatctg ttctgcattt
    32341 ggatggttat tcaattatct ttccgttcaa ttaacaaata tcacgtttgt caatttagat
    32401 acggtcaaaa gtattacgga tatcattagt tccgtttgtc atggatttgc acttatttct
    32461 cttctacttg ccttattttt ggttggatta gaaataatac aacggttgaa aacagatagt
    32521 ctttggaact atatccaatc tgtctatcac acttttctgt tgcgacactt cctgtttcag
    32581 cgtgaacgag tacagaagat aagcaatctt gaacatcaga cggttactac tatcaaccca
    32641 atccataacg gatttaaccg agctgttcgt aagtgtatcg tagatatacg aaaagatacc
    32701 atcactattt ttctaaaagt ccctagagac caacaagggc aaaaaatcct aaaagagatg
    32761 gaagcacaat taaaagaaga aatcgtcagc caacacgcag actattattt ctcctctcct
    32821 attcgagttc gtaaccaatt gtggttcact ggacaaaaac gttaatccct tggggctgtg
    32881 gcttgcgtta agcaaaagcc agacagcccc tttttatatt cttttctatc atgacctatt
    32941 ggggtgacta cgacccccaa taggtcaata atcaataatc acaaaaaatt caattttttc
    33001 aaaaaaactt tgcatacgac ctgtcatttc tccttagtaa tagaacccaa aagaaatgag
    33061 gtaactgcct atggcaaaag aacagcgctc aaccaaatgg acttttctct tctacgagga
    33121 aagcgcccca gagaactact tgaatatatt agaggaactc catattccgt ttattcttag
    33181 tccttggcac gataaggacg ttaatagaca gacgggagag ttcaagaaat cgcacaaaca
    33241 cggtgctttc ttctttgatt cactgaaaag ctattcacaa gtatcaaata tcatcagtga
    33301 caaactaaac ggtccagcac atgtggaggt agttatgtct cccactggtt tatttgacta
    33361 ctttacccat gcggaaaatc cagataagac cccctacaac attgaggata ttgaagtcgg
    33421 ttgcggcttt aatttagaaa aattcttgat ggagatgaac tcctcggatt ttatacatga
    33481 agtagttgat attatcgaag agaacgactt taccgaattt gaagaattgg tctggtatgc
    33541 ccgagcaaac aataccaatt tattagggct tatcattgaa cgtacatatt tcttcgctaa
    33601 atacctagat tcacgaagat acaatcctaa tcgactccat aattcaaaca cagaggagaa
    33661 agagaataat gaataacgaa aaattagaac aaattgaaca attattgcaa acacttatca
    33721 aactcattca aatcaaagaa cagaacatac cactaaagat aatacaacag cgagaattgc
    33781 taaaacagct aaatatctcc ccaaacacac taaaaacttg ggaacaaaaa ggattaaaaa
    33841 gattagaacc tcctatagag ggaactcgaa ccgtcttcta tctactagat gatattatca
    33901 actttttaca atcctaattc tgaaaatttg tgctaaaata gttttatcta aaagaaagag
    33961 tggtaactat tttagcctac ttcaatatcc atgaagaaag gcaaatatgg aaactgaaac
    34021 aattattcat aatggcaaaa aagtcattaa aaaaatcaaa aaagataagt ccatctcata
    34081 cactttgaag gggacatttc tgggaaaaga tatcaaaact ggtaagcaag tcactactac
    34141 cattacagcc aagacactaa aacagttaga tagagcaatc attcaagcac gactagaatt
    34201 tgaaaaaaat ggctcaacac gagaaaaagt tatcgtcatt gatacactgg aagatttagc
    34261 tgaagaatgg tttaaatcat ataaaacctg ggttaactcg cataatactc tgaacagagt
    34321 aagaggttat cttgataatt atattatccc taaatttgga gattacaaac cagataaaat
    34381 tgaatctgcg gatatccagc tttggttaaa cgatttggca aagaaatcaa aagagtccat
    34441 tgaatctggt ataaagcgtg ctgataaagg tagtgctaaa gattttggtg ctgttatcca
    34501 taagttgaaa gatatttttg actatggaat tacaaactat ggactaatca ctaatcctgt
    34561 tagcactgtt aagataccac ctaagccaaa atcaaataag caacgcatca tggtattaca
    34621 tgatgatggt cttgtaatat ggcttaatta tcttgaaagt ttagataaca atagagccaa
    34681 tcgtagattt aagttaattt gtaatactct attagcctcc gctttgcgaa taaatgaatt
    34741 acttgcattg acgattgatg acctagattt tgaaaattct tctattaacg tcagtaaaac
    34801 attgatgtgg aaaaccgcaa ataaaaaaga tgggacaaaa ggggaagtta tctgtaaagc
    34861 tacaccaaaa actgatgcag gaaatcgctc tgtccctgtc cctcccccca taatagaaaa
    34921 actcagagat ttccaccacg aaatgaacaa gtatcttgaa ttgcacaaca gacatagaac
    34981 aaaactgatt ttccctacaa tctatgggaa ctatatgtgt gacagaaacg agagaacaac
    35041 tcttaagaaa aggttagtag gtcttggatt accaaactat ggtttccatt tattccgaca
    35101 tactcatgct tcaatgctac ttaatgctgg aacaaattgg aaagaactcc aagtcagaat
    35161 gggacataaa tccatttcaa ctacaatgga tacttacgct gaattagcac cacaacgaaa
    35221 gttagaagct gtggggattt atcttgaaaa gattgctgag ctaacgcaat aactactcta
    35281 ccattcactc taccttttat tatgtagcat tctagaaaca ttgatgtacc aacgtttcta
    35341 acagctaaaa tagaaa
//
LOCUS       DBJYSH010000004        34543 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.004, whole genome shotgun sequence.
ACCESSION   DBJYSH010000004 DBJYSH010000000
VERSION     DBJYSH010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 34543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34543
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.004"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            <1..502
                     /locus_tag="ACUBV3_000179"
     CDS             <1..502
                     /locus_tag="ACUBV3_000179"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HHU3846717.1"
                     /translation="LAIIKSLPLNDCWLCAGTLRNFIWNKLSGINETLTSDIDVVFFD
                     KNISYEETVVLEQQLKDNYPQYDWELKNEFYMNTHSPNTPKYTSSKDAISKFPEKCTA
                     VGARLDDRNQLELYLPYGEEEILNFIVSPTPYFEEDLLRYNVYLKRVDKKKWNNIWPR
                     LTIIKK"
     gene            722..1141
                     /locus_tag="ACUBV3_000180"
     CDS             722..1141
                     /locus_tag="ACUBV3_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000784700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjdF family protein"
                     /protein_id="HHU3846718.1"
                     /translation="MKMTVYFDGNFWLGLIEYDDDGDYKVFRYFFGKEPKDDDVFNFI
                     NHKLNDLIKKYEFVKTDISLKRTNEHKKSPKRMQREINREKRKPVVSTKAQLAMKTIH
                     MSIKNERQLSQKCKKNELRKHRYQLKQEKRYQKKKGH"
     gene            1341..1820
                     /locus_tag="ACUBV3_000181"
     CDS             1341..1820
                     /locus_tag="ACUBV3_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HHU3846719.1"
                     /translation="MRDFEELFDKVKPIVMKLRRNYFVQLWEYDDWIQEGRIVLFRLL
                     EEHPYLLDNESKLFIYFKTKFSNYLNDVLRHQDCQKRQFNKMPYEEISEVSHYVKSKG
                     LVLDDYIAYRDTLTKVEETLSDIDKEKFEKLISGERFAGKKQFIRDIQPFFNAFKAD"
     gene            1942..2634
                     /locus_tag="ACUBV3_000182"
     CDS             1942..2634
                     /locus_tag="ACUBV3_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000049270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HHU3846720.1"
                     /translation="MSKVRLYIARHGKTMFNTIGRAQGWSDTPLTTFGELGIKELGLG
                     LKASNISFKEAFSSDSGRTLQTMEIILREVQQENIPYTRDKRIREWCFGSLDGGYDGD
                     LFNGVLPRVSNGDMSHLTHEEIANLICQVDTAGWAEPWAILSNRILSGFTAIAKKIED
                     IGGGNAIVVSHGMTIATFLWLIDHSTPRSLGIDNGSVSVVDFEDGTFSIQSIGDMSYR
                     EKGREILEKTLQ"
     gene            2631..3383
                     /locus_tag="ACUBV3_000183"
     CDS             2631..3383
                     /locus_tag="ACUBV3_000183"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HHU3846721.1"
                     /translation="MKKNKIIRFSLVGVLLAILCFSLFALLKPNSQQSSSQKLRNEDI
                     KKISSQKRNKKLQLPAVSSKDWNLILVNRDHKHEELSPDVVPVENIYLDKRITKQATQ
                     FLEAARAIDSREHLISGYRSVAYQEKLFNSYVTQEMTSNPNLTRGQAEKLVKTYSQPA
                     GASEHQTGLAMDMSTVDSLNESDPRVVSQLKKIAPQYGFVLRFPDGKTAETGVGYEDW
                     HYRYVGVESAKYMAKHHLTLEEYITLLKENNQ"
     gene            3380..3955
                     /locus_tag="ACUBV3_000184"
     CDS             3380..3955
                     /locus_tag="ACUBV3_000184"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HHU3846722.1"
                     /translation="MRKRFSLLNFIVVTFIFFFFILFPLLNHKGKVDANSRQSVTYTK
                     EEFIQKIVPDAQDLGKSYGIRPSFIIAQAALDSDFGEKILANKYHNLFGLLAEPGTPS
                     ITLNDSSTGKKQEKQFTHYKSWKYSMYDYLAHIKSGATGKKDSYTIMVSVKNPKTLVQ
                     KLQDSGFDNDKKYAKKMTEIIDLYDLTRYDK"
     gene            4098..5132
                     /gene="hrcA"
                     /locus_tag="ACUBV3_000185"
     CDS             4098..5132
                     /gene="hrcA"
                     /locus_tag="ACUBV3_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015911815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HHU3846723.1"
                     /translation="MITQRQNDILNLIVELFTQTHEPVGSKALQRTIESSSATIRNDM
                     AKLEKLGLLEKAHTSSGRMPSPAGFKYFVEHSLRLDSIDEQDIYHVIKAFDFEAFKLE
                     DMLQKASHILSEMTGYTSVILDVEPARQRLTGFDVVQLSNHDALAVMTLDESKPVTVQ
                     FAIPRNFLTRDLIAFKAIVEERLLDGSVMDIHYKLRTEIPQIVQKYFVTTDNVLQLFD
                     YVFSELFLETVFVAGKVNSLTYSDLSTYQFLDNEQQVAISLRQGLKEGEMASVQVADS
                     QEAALADVSVLTHKFLIPYRGFGLLSLIGPIDMDYRRSVSLVNIIGKVLAAKLGDYYR
                     YLNSNHYEVH"
     gene            5135..5707
                     /gene="grpE"
                     /locus_tag="ACUBV3_000186"
     CDS             5135..5707
                     /gene="grpE"
                     /locus_tag="ACUBV3_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HHU3846724.1"
                     /translation="MAVFNKLFKRRHSVSEEIKKDDLQEEVEATETEETVEEVIEEIP
                     EKSELELANERADEFENKYLRAHAEMQNIQRRSSEERQQLQRYRSQDLAKAILPSLDN
                     LERALAVEGLTDDVKKGLEMTRDSLIQALKEEGVEEVEVDSFDHNFHMAVQTLPADDE
                     HPADSIAEVFQKGYKLHERLLRPAMVVVYN"
     gene            5888..7717
                     /gene="dnaK"
                     /locus_tag="ACUBV3_000187"
     CDS             5888..7717
                     /gene="dnaK"
                     /locus_tag="ACUBV3_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HHU3846725.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGTESKIIANPEGNRTTPSVVSFKN
                     GEIIVGDAAKRQAVTNPDTVISIKSKMGTSEKVSANGKEYTPQEISAMILQYLKGYAE
                     DYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGMDKTDK
                     DEKILVFDLGGGTFDVSILELGDGVFDVLATAGDNKLGGDDFDQKIIDFLVEEFKKEN
                     GIDLSQDKMALQRLKDAAEKAKKDLSGVTQTQISLPFITAGSAGPLHLEMSLSRAKFD
                     DLTRDLVERTKTPVRQALSDAGLSLSEIDEVILVGGSTRIPAVVEAVKAETGKEPNKS
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTSK
                     SQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDIDK
                     NGIVSVKAKDLGTQKEQHIVIQSNSGLTDEEIDKMMKDAEANAEADAKRKEEVDLKNE
                     VDQAIFATEKTIKETEGKGFDTERDAAQSALDELKKAQESGNLDDMKAKLEALNEKAQ
                     ALAVKLYEQAAAAQQAAQGAEGAQSADSSSKGDDVVDGEFTEK"
     gene            8006..9145
                     /gene="dnaJ"
                     /locus_tag="ACUBV3_000188"
     CDS             8006..9145
                     /gene="dnaJ"
                     /locus_tag="ACUBV3_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HHU3846726.1"
                     /translation="MNNTEFYDRLGVSKDASQDEIKKAYRRMSKKYHPDINKETGAEE
                     KYKEVQEAYETLSDTQKRAAYDQYGAAGANGGFGGFDGGGFGGFDGGGFGGFEDIFSS
                     FFGGGGMRNPNAPRQGDDLQYRVNLSFEEAIFGAEKEVSYNRESSCHTCSGSGAKPGT
                     SPVTCQKCHGSGVINVDTQTPLGTMRRQVTCDVCQGSGQEIKEKCPTCHGTGHEKKTH
                     KVSVKIPAGVETGQQIRLTGQGEAGFNGGPYGDLFVIINVLPSQQFERNGSTIYYTLK
                     ISFVQAALGDTIDIPTVHGAVEMSIPAGTQTGKTFRLRGKGAPKLRGGGQGDQHVTVN
                     IVTPTKLNDAQKEALHAFAEASGDKMVHPKKKGFFDKVKDALDVD"
     gene            9263..10510
                     /locus_tag="ACUBV3_000189"
     CDS             9263..10510
                     /locus_tag="ACUBV3_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HHU3846727.1"
                     /translation="MVTKVEEIRSYLIASIQNGKLAPGDRLPSIRQLANQFSCNKDTV
                     QRVLMELRFDNYVYAKPRSGYYVFDSHEEEVEEGVSLPNSEIANIAYDDFRLCLNETL
                     IGREDYLFNYYYRQEGLLDLSKAVAKLMEETGVYVPLDDIVITAGTQQALFILTQVTF
                     PNRKSRVLIEEPTYPRMIELIKTQNLPYETISRGTHGIDFQRLEEIFQTQSIKFFYVI
                     PRMHNPLGTSYNPVEMKRLIEMAEKYDVYIVEDDYMSDFASQSPLHYYDTHGRVIYLK
                     SFSKAIFPALRLAAICLPQALKSTFMAYKKLMDYDTNLILQKALALYIENGLYGKNSQ
                     YLKYRYQKDLANSKSILADQPNLPPYSLHHDSVLFDCSKLDNFKILRQYGDTLENYFC
                     QKSHQSLLQVKNDSCLKQFLGSL"
     gene            10581..11357
                     /gene="truA"
                     /locus_tag="ACUBV3_000190"
     CDS             10581..11357
                     /gene="truA"
                     /locus_tag="ACUBV3_000190"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HHU3846728.1"
                     /translation="MTRYKAQISYDGSAFSGFQRQPNCRTVQEEIERTLKRLNSGNDV
                     IIHGAGRTDAGVHAYGQVIHFDLPQARDVEKLRFGLDTQCPDDIDIVKVEQVSDDFHC
                     RYDKHIKTYEFLVDIGRPKNPMMRNYATHYPYPVIIELMQEAIKDLVGTHDFTGFTAS
                     GTSVENKVRTIFDAKIQFEASKNLLIFTFTGNGFLYKQVRNMVGTLLKIGNGRMPISQ
                     IKTILQAKNRDLAGPTAAGNGLYLKEIIYEDKECFSNFRK"
     gene            11320..12078
                     /locus_tag="ACUBV3_000191"
     CDS             11320..12078
                     /locus_tag="ACUBV3_000191"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HHU3846729.1"
                     /translation="MKTRNVLAISGNDIFSGGGLHADLATYVVNKLHGFVAVTCLTAM
                     SDKGFEVIPIEASILKQQLESLKDVEFGSIKLGLLPNVETAQVVLEFVKSKQECPVVL
                     DPVLVCKENHDLEVSQLREQLIAFFPYADVITPNLVEAQLLTGLSIENLDQMKIAAEK
                     LYDMGAKHVVIKGGNRLNAEEATDLYYDGERFETYVFPVVDANNTGAGCTFASSIASQ
                     LAMGKNVEDAVKMSKGFVYQAIKASDKYGVVQHF"
     gene            12088..12552
                     /locus_tag="ACUBV3_000192"
     CDS             12088..12552
                     /locus_tag="ACUBV3_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HHU3846730.1"
                     /translation="MRKEKTSQLVQLAIVTAISIVLGMFISIPTPTGFLTLLDAGIFF
                     AAFYFGKKEGAVVGALAGFLIDLLKGYPNWMFFSLLIHGAQGYLAGLPGRRRLLGLIS
                     ATLVMVLGYAIASGLMYGWGAVLPDIPGNIMQNMVGMVVGFALNKSLERVKK"
     gene            12549..13118
                     /locus_tag="ACUBV3_000193"
     CDS             12549..13118
                     /locus_tag="ACUBV3_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000599673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HHU3846731.1"
                     /translation="MILDYNKLKQETKAIVVDIIERSALKKGQIFVLGLSSSEVSGGL
                     IGKNSSSEIGEIIVEVILKELHSRGIYLAVQGCEHVNRALVVEAELAERQQLEVVNVV
                     PNLHAGGSGQVAAFKLMTSPVEVEEIVAHAGIDIGDTSIGMHIKRVQVPLIPISRELG
                     GAHVTALASRPKLIGGARAGYTSDPIRKF"
     gene            complement(13155..13997)
                     /locus_tag="ACUBV3_000194"
     CDS             complement(13155..13997)
                     /locus_tag="ACUBV3_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HHU3846732.1"
                     /translation="MITLEKFIDHLNVEEVLFTFFTKLISILLLIIAFVIVRQVINYL
                     FEKTVNRSLAFSRQKVARQKTLAKLSHNVLNYTLYFFLFYWILSILGVPISSLLAGAG
                     IAGVAIGLGAQGFLSDVVNGFFILLENQFDVGDIINVGTVSGTVTNVGIRTTQIHDFD
                     GTLHFIPNRNITIVSNKSRSNMRAQIDIPLFVHTNLDQISDIVTKINEEYVSKHPAIV
                     GEPTVFGPTTNANGQFVYRINIFTQNGAQFDIYAEFYKLYQKAILEEGIDLPTYNFSN
                     NHSR"
     gene            14154..15437
                     /gene="tig"
                     /locus_tag="ACUBV3_000195"
     CDS             14154..15437
                     /gene="tig"
                     /locus_tag="ACUBV3_000195"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HHU3846733.1"
                     /translation="MSTSFENKATNRGIITFTISQDEINPALDQAFNKVKKDLNVPGF
                     RKGHMPRTVFNQKFGEEALYENALNLVLPKAYEAAVAELGLDVVAQPKIDVVSMEKGQ
                     DWKLTAEVVTKPEVKLGDYKDLSVEVDASKEVSAEEVDAKVERERNNLAELTVKDGEA
                     AQGDTVVIDFVGSVDGVEFDGGKGDNFSLELGSGQFIPGFEEQLVGSKAGQTVDVNVT
                     FPEDYQAEDLAGKDAKFVTTIHEVKTKEVPALDDELAKDIDDEVETLDELKAKYRKEL
                     ESAKEIAFDDAVEGAAIELAVANAEIVELPEEMVHDEVHRAMNEFMGNMQRQGISPEM
                     YFQLTGTTEEDLHKQYQADADKRVKTNLVIEAIAAAEGFEATDEEIEKEITDLASEYN
                     MEADQVRGLLSADMLKHDIAMKKAVDVITSSATVK"
     gene            15626..16201
                     /gene="rpoE"
                     /locus_tag="ACUBV3_000196"
     CDS             15626..16201
                     /gene="rpoE"
                     /locus_tag="ACUBV3_000196"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HHU3846734.1"
                     /translation="MELEVFAGQEKSELSMIEVARAILEQRGRDNEMYFSDLVNDIQT
                     YLGKSDSAIRESLPFFYSDLNTDGSFIPLGENKWGLRSWYAIDEIDEEIITLEEDEDG
                     APKRKKKRVNAFMDGDEDAIDYNDDDPEDEDFTEETPSLEYDEENPDDEKSEVESYDS
                     EINEIIPDEDLDEDVEINEEDDEEEEEEEEV"
     gene            16474..18078
                     /locus_tag="ACUBV3_000197"
     CDS             16474..18078
                     /locus_tag="ACUBV3_000197"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HHU3846735.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NIDPGTMSPYQHGEVYVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLKKE
                     RRGEYLGATVQVIPHVTDALKEKIKRAATTTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGSDNVMYIHTTLLPYLKAAGEMKTKPTQHSVKELRGLGIQPNMLVIRTEQPAG
                     QSIKNKLAQFCDVAPEAVIESLDVDHIYQIPLNMQAQNMDQIVCDHLKLETPAADMTE
                     WSAMVDKVMNLEKKVKIALVGKYVELPDAYLSVVEALKHSGYVNDVAIDLKWVNAAEV
                     TEDNIKELVGDADGIIVPGGFGQRGSEGKIEAIRYARENDVPMLGVCLGMQLTCVEFA
                     RNVLKLHGANSAELDPKTPFPIIDIMRDQIDIEDMGGTLRLGLYPCKLKAGSRAAAAY
                     NNQEVVQRRHRHRYEFNTKFREQFEAAGFVFSGVSPDNRLMEVVELPEKKFFVAAQYH
                     PELQSRPNHAEELYTAFVTAAVENMK"
     gene            18187..19113
                     /locus_tag="ACUBV3_000198"
     CDS             18187..19113
                     /locus_tag="ACUBV3_000198"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HHU3846736.1"
                     /translation="MKKIRLSKFIKMIVVILFLISVAASFYFFHVAQVRDDKSFISNG
                     QRKPGNSLYAYDKSFDKLLKQKIEMTNQNIKQVAWYVPAAKKTHKTAVVVHGFANSKE
                     NMKAYGWLFHKLGYNVLMPDNIAHGESHGQLIGYGWNDRENIIKWTEMIVDKNPSSQI
                     TLFGVSMGGATVMMASGEKLPSQVVNIIEDCGYSSVWDELKFQAKEMYGLPAFPLLYE
                     VSTISKIRAGFSYGQASSVEQLKKNNLPALFIHGDKDNFVPTSMVYDNYKATAGKKEL
                     YIVKGAKHAKSFETEPEKYEKRISSFLKKYEK"
     gene            19160..19245
                     /locus_tag="ACUBV3_000199"
     tRNA            19160..19245
                     /locus_tag="ACUBV3_000199"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19194..19196,aa:Leu,seq:aag)
     gene            19308..19754
                     /locus_tag="ACUBV3_000200"
     CDS             19308..19754
                     /locus_tag="ACUBV3_000200"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000049187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HHU3846737.1"
                     /translation="MSKVRGFELVSQFSNKELLPKRETAHAAGYDLKVAKKTVIEPGE
                     ITLVPTGIKAHMQPGEVLYLYDRSSNPRKKGIVLINSVGVIDGDYYNNQVNEGHIFAQ
                     MQNITDQAVILEEGERIVQAVFAPFLLADDDQATGMRTGGFGSTGK"
     gene            19916..21280
                     /gene="radA"
                     /locus_tag="ACUBV3_000201"
     CDS             19916..21280
                     /gene="radA"
                     /locus_tag="ACUBV3_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HHU3846738.1"
                     /translation="MAKKKSVFTCQECGYQSPKYLGRCPNCSAWSSFVEEVEVQEVKN
                     ARVSLNGEKSRPTKLKDVSSINYSRTKTDMDEFNRVLGGGVVPGSLVLIGGDPGIGKS
                     TLLLQVSTQLANKGTVLYVSGEESAEQIKLRSERLGDIDNEFYLYAETNMQSIRSEIE
                     KIKPDFLIIDSIQTIMSPEVSSVQGSVSQVREVTAELMQLAKTNNIATFIVGHVTKEG
                     TLAGPRMLEHMVDTVLYFEGERHHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNP
                     SQVFLEERLDGATGSAIVVTMEGTRPILAEVQALVTPTVFGNAKRTTTGLDFNRVSLI
                     MAVLEKRCGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKEKPTNPKESFIGE
                     IGLTGEIRRVTRIEQRINEASKLGFTKIYAPKNSLAGIEIPKGIDVIGVTTVSQVLKA
                     VFST"
     gene            21416..21913
                     /locus_tag="ACUBV3_000202"
     CDS             21416..21913
                     /locus_tag="ACUBV3_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-class carbonic anhydrase"
                     /protein_id="HHU3846739.1"
                     /translation="MTTYFDNFLKTNQAYADLHGTAHLPIKPKTKVAIVTCMDSRLHV
                     AQALGLDLGDAHILRNAGGRVTDDVLRSLVISQQQLGTREIVVLHHTDCGAQTFTNEA
                     FATQLQRDLGVDMHGHDFLPFNDIEESVREDVAKLHASPLIPNDVVISGAIYDVDTGR
                     MVEVK"
     gene            22067..23386
                     /locus_tag="ACUBV3_000203"
     CDS             22067..23386
                     /locus_tag="ACUBV3_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HHU3846740.1"
                     /translation="MKKYDVIVLGFGKAGKTLAAKLATQGKSVAMVEEDDKMYGGTCI
                     NIGCIPTKTLLVSASKNHDFQEAMTTRNEVTSRLRAKNFAMLDNKDTVDVYNAKARFI
                     SNKVVELTGGADKQELTADVIIINTGAKSVQLPIPGLADSQHVYDSTAIQELAHLPKR
                     LGIIGGGNIGLEFATLYSELGSKVTVIDSQSRIFAREEEELSEMAQDYLEEMGISFKL
                     SADIKSVQNEDEDVVISFEDEKLSFDAVLYATGRKPNTEGLALENTDIKLTERGAIAV
                     DEYCQTSVENIFAVGDVNGGPQFTYISLDDSRIVLNYLNGDKDYSLKNRGAVPTSTFT
                     NPPLATVGLDEKTAKEKGYQVKSNSLLVSAMPRAHVNNDLRGIFKVVVDTETNLILGA
                     RLFGAESHELINIITMAMDNKIPYTYFQKQIFTHPTMVENFNDLFNF"
     gene            23595..25049
                     /gene="gltX"
                     /locus_tag="ACUBV3_000204"
     CDS             23595..25049
                     /gene="gltX"
                     /locus_tag="ACUBV3_000204"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HHU3846741.1"
                     /translation="MANKIRVRYAPSPTGLLHIGNARTALFNYLYARHHGGDFVIRIE
                     DTDRKRHVEDGERSQLENLRWLGMDWDESPETHENYRQSERLELYQRYIDQLLAEGKA
                     YKSYVTEEELAAERERQELAGETPRYINEFIGMSETEKEAYIAEREASGIIPTVRLAV
                     NESGIYKWTDMVKGDIEFEGSNIGGDWVIQKKDGYPTYNFAVVIDDHDMQISHVIRGD
                     DHIANTPKQLMVYEALGWEAPQFGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYM
                     SEAVFNFIALLGWNPGGEEEIFSREQLINLFDENRLSKSPAAFDQKKMDWMSNDYLKN
                     ADFESVFALCKPFLEEAGRLTDKAEKLVELYKPQLKSADEIVPLTDLFFADFPELTEA
                     EKEVMAAETVPTVLSAFKEKLVSLSDEEFTRDTIFPQIKAVQKETGIKGKNLFMPIRI
                     AVSGEMHGPELPDTIYLLGKEKSVQHIDNMLAKL"
     gene            complement(25089..26057)
                     /locus_tag="ACUBV3_000205"
     CDS             complement(25089..26057)
                     /locus_tag="ACUBV3_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HHU3846742.1"
                     /translation="MKFGKKLGFLALLMSIVLILGACGKTGLGNSTGNSTKNVTKKSA
                     KNLKLGVSISTTNNPYFVAMKDGIDKYASNKKISIKVADAQDDAARQADDVQNFISQN
                     VDAILINPVDSKAIVTAIKSANNANIPVILMDRGSEGGKVLTTVASDNVAAGKMAADY
                     AVKKLGKKAKAFELSGVPGASATVDRGKGFHSVAKSKLDILSSQSANFDRAKALNTTQ
                     NMIQGHKDVQIIFAQNDEMALGAAQAVKSAGLQNVLIVGIDGQPDAHDAIKKGDISAT
                     IAQQPAKMGEIAIQAAIDHYKGKKVEKETISPIYLVTKDNVEKYNW"
     gene            complement(26110..27051)
                     /locus_tag="ACUBV3_000206"
     CDS             complement(26110..27051)
                     /locus_tag="ACUBV3_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HHU3846743.1"
                     /translation="MKNMMKYISELTTLIALAGLMVIITLINPNFLTTNNLLNLLLQV
                     TANGFIAFGMTFVILTGGIDLSVGSILALSSALTAGLIGHGVPVGIAIVLSVALGGIL
                     GMLNGLFISYGKLAPFIVTLATMTIFRGATLVYSNGNPITAGLSDSFLFQFLGQGYIV
                     GIPFPVILMFLTFIILYILLHKTAFGKSVYALGGNEKAAYISGIKLNKVKIIIYTISG
                     IMASISGLIITSRLSSAQPTAGASYEMDAIAAVVLGGTSLSGGKGRIIGTLIGALIIG
                     VLNNGLNIIGVSAFWQQVVKGIVILMAVLLDRFKVAK"
     gene            complement(27053..28531)
                     /locus_tag="ACUBV3_000207"
     CDS             complement(27053..28531)
                     /locus_tag="ACUBV3_000207"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HHU3846744.1"
                     /translation="MKIDMRNISKSFGTNKVLEKIDLELQSGQIHALMGENGAGKSTL
                     MNILTGLFPASTGTIYIDGEERTFSNPQEAEEFGISFIHQEMNTWPEMTVLENLFLGR
                     EIKTTFGLLNQKLMRQKALEAFKRLGVTIPLDIPIGNLSVGQQQMIEIAKSLLNQLSI
                     LVMDEPTAALTDRETENLFRVIRSLKQEGVGIVYISHRMEEIFKITDFVTVMRDGVIV
                     DTKETSLTNSDELVKKMVGRKLEDYYPEKHSEIGPVAFEVSNLCGDNFEDVSFYVRKG
                     EILGFSGLMGAGRTEVMRTIFGIDKKKSGKVKIDNQEITITSPSQAIKQGIGFLTENR
                     KDEGLILDFNIKDNMTLPSTKDFSKHGFFDEKTSTTFVQQLINRLYIKSGRPDLEVGN
                     LSGGNQQKVVLAKWIGIAPKVLILDEPTRGVDVGAKREIYQLMNELADRGVPIVMVSS
                     DLPEILGVSDRIMVMHEGRISGELSRKEADQEKVMQLATGGK"
     gene            complement(28547..28945)
                     /gene="rbsD"
                     /locus_tag="ACUBV3_000208"
     CDS             complement(28547..28945)
                     /gene="rbsD"
                     /locus_tag="ACUBV3_000208"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HHU3846745.1"
                     /translation="MKKTGILNSHLAKLADDLGHTDRVCIGDLGLPVPNGIPKIDLSL
                     TSGIPSFQEVLDIYLENILVEKVILAEEIKEANPDQLSRLLAKLDNSVSIEYVSHDHL
                     KQMTQDVKAVIRTGENTPYSNIILQSGVII"
     gene            complement(28920..29831)
                     /gene="rbsK"
                     /locus_tag="ACUBV3_000209"
     CDS             complement(28920..29831)
                     /gene="rbsK"
                     /locus_tag="ACUBV3_000209"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HHU3846746.1"
                     /translation="MSNIVIIGSISMDLVMETNRIAKEGETVFGQRFSMVPGGKGANQ
                     AVAIGRLSQDRDNITILGAIGEDSFGPILLDNLNKNHVTTDFVGTIPSSSGVAQITLY
                     NNDNRIIYCPGANGKVDTKKWSQEWSIIKEADLVVLQNEIPHQANMKIANFCKEHSIK
                     LLYNPAPSRKTDIEMLDKVDYFTPNEHECQELFPNQKLEDILATYPEKLIVTLGTKGA
                     IYSDGKESHLIPALETKAVDTTGAGDTFNGAFGYAISKKFKIANALRFATLAAHLSVQ
                     KFGAQGGMPTIKEMEDNQHYEKDWHIK"
     gene            complement(29824..30810)
                     /locus_tag="ACUBV3_000210"
     CDS             complement(29824..30810)
                     /locus_tag="ACUBV3_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HHU3846747.1"
                     /translation="MSTIRQVAEKAGVSTSTVSRYISQNGYVSQKASQKIEQAIRELH
                     YVPNFLAQSLKTKKNQLVGLLLPDISNPFFPRLARGVEEFLKEQGYRVMLGNTNNKSH
                     LEEEYLNVLLQSNAAGIITTHDFTKNHPEIDIPVVVVDRVNQETQYGVFSDNKEGGKL
                     AAQAIWTAGATNILLIRGPLDKADNLNQRFQGSQNYLLNKGARFAIEDSASFDFAEIQ
                     IEAKTLLDHHPDIDSIIAPSDIHAIAYLHEILNRGKRIPEDVQIIGYDDILMSQFIYP
                     SLSTIHQSSYIMGQKAAELIFKITNQLPITNKRIKLPVHYVERETLRRKIDE"
     gene            30992..32080
                     /locus_tag="ACUBV3_000211"
     CDS             30992..32080
                     /locus_tag="ACUBV3_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HHU3846748.1"
                     /translation="MSHAWKELTFYKKKYLLIELLIIVMMFMVVFLSGLANGLGRAVS
                     AAIENNPAQTYILNEGAEQVITSSVLTTKDQTDLNSLNLKDSTTLNIQRSSLTRQGHE
                     KKIDISYFAIDKDSFMAPTLSEGKQLTSYKKAIILNDSLKAEGIKLGDKVIDKSSSIS
                     LTVVGFVHNSMYGHGPVAFIDKDIYTEINKKINPQYQFLPQALVMKNDKSISHLPTQL
                     EAVSKKDVIQHIPGYSAEQSTLNMILWVLVVASAGILGVFFYIITLQKRHEFSVMKAI
                     GTKMSEIALFQLSQVIILALFGIIVGDGLAIALSYVLPAQMPFVINWQNIILVSFVFL
                     VIAMISSALSIVKVAKIDPVEVINGGGE"
     gene            32080..32766
                     /locus_tag="ACUBV3_000212"
     CDS             32080..32766
                     /locus_tag="ACUBV3_000212"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HHU3846749.1"
                     /translation="MSAILELKHISKHYPDGDELLSILDNLDLSVSAGEFVAILGPSG
                     SGKSTLLSIAGLLLGADQGSLYVNHENVTDLSQRQRTQLRREALGFIFQSHQLLPYLT
                     IQEQLQQEARFAKHYDKKTSLEEINKLLSDLGIEQCAHKYPNQLSGGQKQRAAIARAF
                     INHPKVILADEPTASLDEERGRQVTELIRQEVKSHNTAAIMVTHDERVLDLVDTVYRL
                     KDGKLVKENF"
     gene            32797..33468
                     /locus_tag="ACUBV3_000213"
     CDS             32797..33468
                     /locus_tag="ACUBV3_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HHU3846750.1"
                     /translation="MIKILVVEDNIVQQKIITTKLTQEGYQFITASNGQEALNCLDTE
                     EVQLIITDIMMPMMDGYQLIQELRSAAYNVPIIVMTAKSQMEDMTKGFGLGADDYMVK
                     PVQLQELALRIKALLRRANIVAQHQLIIGNTCLNEDELSLKYFEQEIVFPQKEFRVLF
                     HLLSYPNRIFTRLELLDSIWGMDTDLDERVVDACINKIRRKVEHLPDFKIETVRGVGY
                     RAKND"
     gene            33452..34531
                     /locus_tag="ACUBV3_000214"
     CDS             33452..34531
                     /locus_tag="ACUBV3_000214"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HHU3846751.1"
                     /translation="MQKMTKLRRFRFPLRFYFTLMFVLTMLFSVLASLLLVAAIVFTF
                     FQGVLTTHVLQVSALAVVFLSLVIASISMWYGSYHLTKPILDISRIVSNVADGDFEGH
                     IYRNSNRRKSYEYYNELDELSESINQMIVSLSHMDHMRKDFITNVSHELKTPIAAVAN
                     IVELLQDPELDEETQSELLGLVKTESLRLTRLCDTMLQMSRVDNQETIGELSSVRVDE
                     QIRQAMISLTERWQAKRINFQLDSKPYTVYSNSDLLMQVWINLLDNAIKYSDDIVDLR
                     VRMEETNNHYLRVIISDKGRGISQYDVQHIFDKFYQADQSHNQQGNGLGLAIVKRIIV
                     LCKGRISVSSQFEIGTEFCVELPLS"
BASE COUNT        11265 a         5494 c         6988 g        10796 t
ORIGIN      
        1 tttagctata ataaaatctc ttccactgaa tgattgttgg ctgtgtgctg gcacattgag
       61 aaactttata tggaataaac tttctggaat aaatgagaca ttgacatcag atattgatgt
      121 tgtatttttt gataaaaata tttcttatga agaaacggtg gtattagaac aacaattaaa
      181 ggataactat ccacagtacg attgggaact taaaaatgaa ttttacatga atactcattc
      241 tcctaatacc ccaaagtata ctagctctaa agatgctatt tcgaaatttc ctgaaaagtg
      301 tactgcagtg ggagcaaggt tagatgatag aaatcaactc gaactttacc tcccttatgg
      361 tgaagaggaa atattaaatt tcattgttag tcctacgcca tattttgaag aggatctatt
      421 acgttataat gtttatttga agcgagtgga taagaagaaa tggaataata tttggcctcg
      481 tttaacaatc ataaaaaaat gataacgtta ctggagttgg cttaacagct tttaaatttt
      541 tagactgaca aatagtatag acctttaata aggtcgttta tcgataatca tagtgtactg
      601 tgttataatc gttatagcat aaaaacacgt tgcagttggt agtctgcaag cgttcagtga
      661 acgtcagtaa ccttccctcc aggtcgtcca ttgcaattta atatttaaag gagggggctg
      721 tatgaaaatg acagtctatt ttgatggtaa tttttggttg ggtttgattg agtacgatga
      781 tgatggtgat tacaaagttt ttcggtattt ttttggaaaa gaacctaaag atgatgatgt
      841 ttttaatttt ataaatcata aacttaatga tttgataaaa aaatatgagt ttgttaaaac
      901 ggatatttct ctaaagcgta cgaatgaaca caaaaaaagt cctaaaagaa tgcaaagaga
      961 aattaataga gaaaaacgta agccagtagt atctacaaaa gcgcaattgg cgatgaaaac
     1021 tattcatatg agtataaaaa acgaacgtca attaagtcag aaatgtaaga aaaatgagtt
     1081 aagaaagcat cggtaccaat taaaacaaga aaaacgttac caaaagaaaa aaggacattg
     1141 attgtgtcat catataaaaa gcttgagagt gtcatctcaa gctttttata tgttaactaa
     1201 tatgagtcac taaatagtct gataatgctc aattaatgtg tttggggaaa aaatcatttc
     1261 cttaaaggac accattgtca tgttgttatc accttacttt ttctatactt agtatatgtt
     1321 taagaaagga ataagttatt ttgagagatt ttgaggagtt gtttgataaa gtaaagccaa
     1381 tcgtaatgaa gctgagaaga aattattttg ttcaactttg ggaatatgat gattggattc
     1441 aagaagggcg tattgtactt tttaggttgc ttgaagaaca tccgtattta ttggataatg
     1501 aaagcaaact attcatttac tttaaaacaa aattttcaaa ttatttaaac gatgtattaa
     1561 gacatcaaga ttgtcaaaag cgacaattta ataaaatgcc ttatgaagaa atcagtgaag
     1621 ttagtcacta tgttaagagt aaaggactag ttttagatga ctatattgct tatcgtgata
     1681 ctttaacaaa agtggaagaa actttgtcag atattgataa agaaaaattt gaaaaactga
     1741 ttagtggtga acgtttcgcg gggaagaaac aattcatacg agatattcaa ccttttttca
     1801 atgcttttaa agcggattaa tcgattttat ttctgcagta gatagctgca gaattttttt
     1861 atagggaaag ttcgctgata tattcctgtg aaagcctttg ttttttgtta taatataata
     1921 aaatgcttgt gaggtgtcag tatgagtaaa gttcgtttat atatcgctcg tcatggtaaa
     1981 acaatgttta atacaattgg tcgcgctcag gggtggagtg atactccttt aactactttt
     2041 ggagaactcg ggattaaaga gcttggtttg ggactaaagg cttcaaatat tagttttaaa
     2101 gaggcttttt ctagtgatag tggacgtact ttacaaacta tggaaattat tctcagggaa
     2161 gtgcaacaag aaaatattcc ctatacgaga gataaacgaa tccgcgagtg gtgctttggt
     2221 agtttggatg gaggatatga tggcgacctt tttaatggcg ttctgcctcg agtttcaaac
     2281 ggagatatga gccatttgac acacgaagaa attgctaatt tgatttgtca agttgatacg
     2341 gctggctggg cagaaccgtg ggcaatattg agtaaccgta ttctgtcagg cttcactgct
     2401 atagctaaaa aaatagagga tattggggga ggtaatgcga ttgttgtcag tcatgggatg
     2461 actattgcga cttttttatg gttaatcgac catagtaccc ctcgtagttt aggcatagat
     2521 aatggtagtg tctcagtcgt tgattttgaa gatggaacat tttctattca atctattggg
     2581 gatatgtcct atcgagaaaa aggaagagaa attttagaaa aaacattgca atgaaaaaaa
     2641 ataaaattat ccgattcagt ttagttggtg ttctacttgc gatactatgc tttagtcttt
     2701 ttgctttatt gaagcctaac agtcaacaat catcatctca aaagttgagg aatgaggata
     2761 taaaaaagat atcctctcaa aaaagaaata agaaattaca attaccagct gtatcatcaa
     2821 aagattggaa cttgattttg gtcaatcgtg accataaaca tgaagaatta agtccagatg
     2881 tggttcctgt tgaaaatatt tatttggata aacgtattac gaagcaagct actcagtttt
     2941 tagaggctgc tagagcaatt gattcacgag aacatttaat ttcgggttat cgtagtgttg
     3001 cctatcagga gaagttgttc aattcttatg ttactcaaga gatgactagt aaccctaatt
     3061 tgacgagggg acaagcagaa aagttggtaa aaacttactc tcagcctgca ggtgctagtg
     3121 aacaccagac tggattagcg atggatatga gtactgtaga ttctttgaat gagagcgatc
     3181 ctagagtagt cagtcagttg aaaaagatag ctccacaata tggttttgtc ttacggtttc
     3241 cggatggtaa aacagcagaa acaggggtag gttatgaaga ttggcattac cgctatgttg
     3301 gggtagagtc tgcaaaatat atggccaaac atcatttaac attagaagaa tacataactt
     3361 tattaaagga gaataaccaa tgaggaaacg tttttccttg ctaaatttta ttgttgttac
     3421 ttttattttc tttttcttta ttctttttcc gcttttaaac cataagggaa aagtagatgc
     3481 taattctagg cagagtgtta cctacaccaa agaagaattt atacaaaaaa ttgtgccaga
     3541 tgcgcaagat ctaggaaagt cgtacggtat tcgtccttca tttattattg cacaggcggc
     3601 tttggattct gatttcggag agaaaatatt agctaataag tatcataatc tgtttggttt
     3661 gcttgcagaa ccaggaacgc cctcaattac cttaaatgat agtagtacag gaaaaaaaca
     3721 ggaaaagcaa tttactcatt ataaatcttg gaagtattca atgtatgatt accttgctca
     3781 tataaaatct ggagcgacag gcaaaaaaga ttcatatact ataatggtgt ctgttaaaaa
     3841 tccaaaaact ttagtgcaaa aattacaaga tagtggtttt gataatgaca aaaagtacgc
     3901 taaaaaaatg acggaaatca ttgatttgta tgatttaaca agatatgata agtgatatta
     3961 gcactctttg aataagagtg ctaatttttg atgttttttc cttgactatc ttttaaaaag
     4021 gagtataata aaatcatagt tagcagtcga tgtgtaagag tgctaatttc agaaagaccg
     4081 agaggggtga tgtttttgtg attacacaac gtcaaaatga tattttgaat ttaatcgttg
     4141 agttatttac acaaactcat gaaccagtag ggtctaaagc tttacagaga acgattgaat
     4201 ctagtagcgc cactattcgt aatgatatgg caaaactaga gaagttgggg ttattggaaa
     4261 aagcacacac ttctagtgga cggatgccga gtccagctgg ttttaagtat tttgtagagc
     4321 actcccttag actagatagt attgatgagc aagatattta tcatgttata aaagcttttg
     4381 actttgaagc attcaaatta gaagacatgc ttcaaaaagc tagtcatatt ttatcggaga
     4441 tgacgggcta tacatccgtt attttagatg tggagccagc tcgtcaacgt ttgacagggt
     4501 ttgatgttgt tcagcttagc aaccatgatg ctctagcagt catgacttta gatgaatcta
     4561 agccagtgac agtgcagttt gctattccaa gaaacttttt gacacgtgac ttgatagcat
     4621 ttaaagctat cgtagaggag cgcttgctgg atggtagtgt catggatatt cattataagt
     4681 tgaggacaga aattcctcag atcgttcaga agtattttgt aactacggat aatgttttac
     4741 aactatttga ttatgttttt tcagagctgt ttctggaaac ggtatttgta gctggcaagg
     4801 taaattcttt aacatattca gatctatcaa cgtatcagtt tttggataat gagcagcaag
     4861 tagcaatttc tttgaggcaa ggtctgaaag agggtgagat ggcttctgtc caagtggcag
     4921 atagtcaaga agccgcactt gcagatgtgt cagttttaac tcacaaattt ttgattcctt
     4981 accgtggatt tggactcctg agtttgattg gtcctattga tatggattat cgtaggtctg
     5041 ttagtttggt aaacattatc ggaaaggtat tggctgctaa attaggtgat tattatcgat
     5101 acctaaattc taaccattat gaagttcact gataatggca gtattcaata agttatttaa
     5161 aaggaggcac tcagtgtcag aagaaattaa aaaagacgat ttacaggaag aagtggaagc
     5221 cactgaaact gaagagacag ttgaggaagt tattgaagaa attcctgaaa aatcagagtt
     5281 agaactcgca aacgagcgtg ctgatgagtt tgaaaataaa tacttgcgtg ctcatgcaga
     5341 aatgcagaat atacagcgtc gctctagtga agaacgtcaa caacttcaac gctatcgttc
     5401 tcaggatctg gcaaaagcaa tcttaccatc gcttgacaat ctagaacgtg ccttggctgt
     5461 tgaagggttg acagatgatg ttaaaaaagg tttagaaatg actcgtgata gccttattca
     5521 agcactaaag gaagaaggcg tagaagaagt agaagtcgac tcatttgacc acaactttca
     5581 tatggcagtt cagacccttc cagccgatga tgaacatcca gccgatagca ttgcagaagt
     5641 ctttcaaaag ggatataaat tgcacgagcg tctgctaaga ccagcgatgg tagttgtcta
     5701 taactaaggt aaatgagttt tcgtttttgt ccgtaatgac agtaaactag atagcaagtt
     5761 agaagctatt cagcttgctg attaaactat agtgattgct tagaattgga agtaaaataa
     5821 ttcgagtgct tactaagata aattgaaata aaaagtaata aagtattata aaataagagg
     5881 tattaacatg tctaaaatta ttggtattga cttaggtaca acaaactcag cagtagcagt
     5941 tcttgaaggg actgaatcaa aaatcattgc taacccagaa ggcaatcgta caactccttc
     6001 agtagtatca ttcaaaaatg gtgaaattat cgtgggtgat gctgcaaaac gtcaagcggt
     6061 aacaaatcca gatactgtta tctctatcaa atcaaagatg ggaacttctg aaaaagtttc
     6121 tgcaaatggt aaagaatata ctcctcaaga aatttcagca atgattcttc aataccttaa
     6181 aggttatgct gaagactatc ttggagaaaa agtagaaaaa gcagttatta ctgttccagc
     6241 ttacttcaac gatgcacaac gtcaggcaac taaagacgct ggtaaaattg caggtcttga
     6301 agtagaacgt atcgttaacg aaccaacagc agccgcactt gcttatggta tggacaagac
     6361 tgacaaggat gaaaaaatct tagtttttga ccttggtggt ggtacatttg acgtatcaat
     6421 ccttgaatta ggtgatggtg tcttcgacgt tcttgcaaca gcaggtgata acaaacttgg
     6481 tggtgacgac tttgaccaga aaattattga tttcttggta gaagaattca agaaagaaaa
     6541 tggtattgat ctttctcaag acaaaatggc tcttcaacgc ttgaaagatg ctgctgaaaa
     6601 agctaaaaaa gacctttcag gtgtaactca aactcaaatt tcattaccgt tcatcactgc
     6661 tggttctgct ggtcctcttc acttggagat gagcttatca cgtgctaaat ttgacgatct
     6721 cactcgtgac cttgttgaac gtacgaaaac tccagttcgt caagctcttt cagatgcagg
     6781 cttgtcattg tcagaaattg atgaagttat cctcgttggt ggatcaacac gtatcccagc
     6841 agttgttgaa gctgtaaaag ctgaaactgg taaagaacca aataaatctg ttaaccctga
     6901 tgaagtggtt gccatgggtg ctgctatcca aggtggtgtt atcactgggg atgtgaaaga
     6961 cgttgtactt cttgacgtaa caccattgtc acttggtatt gaaacaatgg gtggtgtctt
     7021 cactaaattg atcgaccgca acacaactat cccaacatct aaatcacaag tcttctcaac
     7081 agcagcagac aaccaaccag ccgttgatat ccatgttctt caaggtgaac gcccaatggc
     7141 agcagataac aaaacactcg gtcgcttcca attgactgat atcccagctg cacctcgtgg
     7201 aatcccacaa attgaagtaa catttgatat cgataaaaat ggtattgtat ctgttaaagc
     7261 taaagatctc ggtactcaaa aagaacaaca cattgttatc caatctaatt caggattaac
     7321 tgatgaagaa attgataaaa tgatgaaaga tgctgaagca aatgctgaag cagatgcaaa
     7381 acgtaaagaa gaagttgatc ttaaaaatga agttgaccaa gccatctttg caacagaaaa
     7441 aactattaaa gaaactgaag gcaaaggttt tgatacagaa cgcgatgcag cgcaatcagc
     7501 acttgatgag ttgaaaaaag ctcaagaatc aggtaacctt gacgacatga aagctaaact
     7561 tgaagctctt aacgaaaaag cacaagctct tgcagttaaa ctttacgaac aagcggctgc
     7621 agcacaacaa gcagctcaag gggctgaagg tgcacaatca gctgattcat caagcaaggg
     7681 tgatgatgtt gtagatggcg aattcactga gaaataatta ttaatattgt tcagattcat
     7741 ttgaatataa gcatgaaaac tatactagca tagtaaagtt cttcgtgata gggattgctc
     7801 aataatctag ataagtttca gattacataa gctaatttcg ctatcactaa ataaaaacat
     7861 attaataata aataggcggg gcgcctcgct ccgtctgttt tattaagtgt catatatatg
     7921 ttaactattt agagctgtaa ctgggcaaga ataattgtta atctcttcaa gtgtagtata
     7981 tgaacaaaat ataaaggatt agataatgaa caatacagaa ttttatgatc gtcttggcgt
     8041 ttcaaaagat gcttctcagg acgaaataaa aaaagcttac cgtaggatgt ctaagaaata
     8101 ccatccagat attaataagg aaacgggagc agaggaaaaa tataaagagg ttcaagaagc
     8161 ttatgaaacc ttgagtgata cacaaaaacg tgctgcctat gatcaatatg gcgctgctgg
     8221 ggcaaatggc ggttttggtg gcttcgatgg tggcggtttt ggtggcttcg atggtggtgg
     8281 ctttggtggt tttgaagaca tcttctcaag cttttttggt ggaggaggga tgcgcaatcc
     8341 taatgcacca cgtcaaggag atgacctaca atatcgtgtt aatttaagtt ttgaagaagc
     8401 tatctttgga gctgaaaaag aagtatctta taatcgggag tcatcatgtc atacttgttc
     8461 aggaagtgga gctaagccag gtactagccc tgtgacttgt caaaagtgtc atggttcggg
     8521 tgtgattaat gttgatacac aaacacctct aggaacaatg cgtcgccaag tgacttgtga
     8581 tgtttgtcag ggtagtggtc aagaaatcaa agagaaatgt ccaacatgtc atggaactgg
     8641 tcatgagaag aagacgcata aagtatcagt taaaatacct gcaggagttg aaactgggca
     8701 acaaattcgt ttaacaggac aaggtgaagc tggcttcaat ggtggtcctt atggtgatct
     8761 ctttgttatt attaacgttc tcccaagcca acagtttgag cgtaatggat caacaattta
     8821 ttatactttg aaaattagct ttgtccaagc tgctttgggt gatacaattg atatacctac
     8881 tgtccatgga gcggtagaaa tgtcaattcc tgctggtact cagacaggaa aaacgttccg
     8941 tcttcgcgga aaaggtgccc ctaaacttcg agggggtgga caaggtgatc agcatgttac
     9001 tgttaacatc gtaactccta ctaaattgaa tgatgctcaa aaagaggcat tgcacgcttt
     9061 tgcagaggca agtggagata agatggtaca tccgaagaag aaaggtttct ttgataaagt
     9121 caaagatgct ttagatgttg attaaaaaat aaaagtctag aatcgaaagt attctagact
     9181 tttttgtagt tattgctaga gggattgctt gaacctaaat tttacagtac aatgaatcta
     9241 atattgtagg gggacaattt ttatggtaac caaagttgaa gagattcgct catatttgat
     9301 agcttctata caaaatggta agttggctcc aggagatcgc ctaccatcta taagacagtt
     9361 agctaatcaa ttttcctgta acaaggatac agtgcaacga gttttgatgg aattgcgttt
     9421 tgataattat gtctatgcaa agcctaggtc aggctattac gtctttgatt ctcatgaaga
     9481 ggaagttgaa gaaggggtta gtttaccaaa ctctgagatt gcaaatatag cttatgatga
     9541 ttttagattg tgtttgaatg agacccttat tggtagggaa gattaccttt tcaattatta
     9601 ctatcgtcaa gaaggtcttc ttgatttaag taaagcggtg gctaaattaa tggaagaaac
     9661 aggggtctat gttcctcttg atgatattgt tattacagct ggtactcaac aggcattatt
     9721 tattttgaca caggttacct ttccaaatcg aaaatctcga gttttaatag aagaaccgac
     9781 ctatcctcgt atgattgaac taatcaaaac acaaaattta ccctatgaaa ctatctctcg
     9841 aggtactcat ggaattgatt ttcagcgttt agaggagatt ttccagacac aatcaattaa
     9901 gtttttttat gttatacctc gcatgcataa tcctttggga acatcctata atccggtaga
     9961 gatgaaaaga ttaatagaga tggcagagaa gtatgatgtt tatattgtgg aagatgacta
    10021 tatgtctgat tttgcaagtc agtcaccatt acattattat gatactcacg ggcgtgttat
    10081 ttatctaaaa tctttttcaa aggctatttt ccctgcttta agattagctg cgatttgttt
    10141 accacaagct ttaaaatcaa catttatggc ttacaagaag ttgatggatt atgatactaa
    10201 tctgatttta caaaaagcat tagcgcttta tattgaaaat ggcctttatg gtaagaatag
    10261 tcaatatttg aaatatcgtt atcagaaaga ccttgcaaat tcaaaatcta ttttagctga
    10321 tcagcctaat ctacccccat atagtttaca tcacgatagt gtattatttg attgttcgaa
    10381 actcgataac ttcaaaatat tacggcaata cggcgatact ttggaaaatt atttttgtca
    10441 aaaatcgcat caatctctct tacaagtaaa aaatgattcc tgcttaaagc agttcttggg
    10501 atcgttgtag tataattgta gggggacagt ttaaaataga tattatagtt ttcataaatg
    10561 aaaggtggta aaataaaccc atgacaaggt ataaggcaca gatttcttat gatggttcag
    10621 ctttttcagg cttccaaaga caaccaaatt gtaggacagt tcaagaagaa attgagagaa
    10681 ctcttaagcg cctaaatagc gggaacgatg tgataatcca tggtgctggt cgcacagatg
    10741 ctggtgttca tgcttatgga caagttattc attttgattt accacaggcg cgtgatgtag
    10801 aaaaattacg ctttggttta gatacacagt gtccagacga tattgatatt gtcaaagtag
    10861 agcaagtgtc tgatgatttt cattgtcgat atgataaaca cattaaaact tatgagtttt
    10921 tagtagatat tggtaggccg aaaaatccta tgatgagaaa ctatgcaaca cattaccctt
    10981 atcctgtcat tattgagctt atgcaagaag ctattaaaga tttagtaggt acccatgatt
    11041 ttacaggctt tactgcttca ggtacgagtg tagaaaataa agtacgtact atttttgatg
    11101 cgaaaattca gtttgaagcg tctaaaaatc ttttaatttt tacctttaca ggaaatggtt
    11161 ttttgtataa acaggtgcgt aacatggttg gtaccctgct aaaaattggt aatggacgca
    11221 tgcctattag ccaaatcaaa acaattttac aggctaaaaa tcgggattta gcaggtccaa
    11281 ctgcggcagg aaatggttta tatttgaagg agattattta tgaagacaag gaatgtttta
    11341 gcaatttcag gaaatgatat tttctctggc ggcggcttgc atgcagattt agcaacttac
    11401 gttgttaata aattacatgg ttttgtggca gtaacatgct taacagcaat gtcagataaa
    11461 gggtttgaag tcattcctat agaggcatct atactaaagc agcaactaga aagtttaaaa
    11521 gatgtagagt ttgggtctat taaactagga ttgcttccca atgtagaaac agcacaagtt
    11581 gttttggaat ttgtaaaatc aaaacaagaa tgtccagtag tcttagaccc agtcttagtt
    11641 tgtaaagaaa atcatgattt agaagttagc caattacgtg agcagctaat tgcgtttttc
    11701 ccatatgctg atgtgattac tcctaattta gtagaggctc aattattaac gggtctttcg
    11761 attgaaaact tagatcaaat gaaaattgct gctgagaaac tttacgatat gggggctaaa
    11821 catgttgtta tcaaaggggg caaccgttta aatgctgaag aggctacaga cctttactat
    11881 gatggtgaac gttttgaaac ctatgttttt cctgtagtag atgccaataa tacaggtgct
    11941 ggatgtacat ttgcgtcaag tattgccagc caattagcta tgggtaaaaa tgtagaagat
    12001 gcagtcaaaa tgtctaaagg tttcgtctat caagctatta aagcatcgga taagtacgga
    12061 gttgtccaac atttttagga gattttaatg aggaaagaga aaactagtca attggttcaa
    12121 ttggcaattg taacagctat atctattgta ttggggatgt ttataagtat tccgacacca
    12181 acaggttttt tgacattact agatgctgga atattctttg ctgcctttta ctttggcaaa
    12241 aaagaagggg ctgtggtagg tgccttagct ggttttttaa ttgatttatt gaaaggttat
    12301 cccaattgga tgttctttag tttattgatt catggggcgc aagggtattt agcgggttta
    12361 ccaggtagaa gacgtctttt aggtcttatt agtgcaactc ttgttatggt attaggttat
    12421 gctatagcta gtggattgat gtatggttgg ggagctgttc taccagatat tcctggcaat
    12481 attatgcaaa atatggttgg tatggtcgtt ggttttgctc ttaataagtc tctagaaaga
    12541 gtgaagaaat gatactagat tataataagt taaagcagga aactaaagct attgtagttg
    12601 atattattga acgtagcgct cttaaaaaag gtcaaatttt tgtactaggt ctatcatcta
    12661 gtgaggtttc tggtggttta attggaaaaa attctagttc tgaaattggt gaaattatcg
    12721 ttgaagttat cctcaaagag ttacattcac gcggcatcta tttagcagtt caaggttgtg
    12781 aacatgttaa tagagcgttg gttgttgaag cagaattggc tgaacgtcaa cagttggaag
    12841 tcgttaatgt tgttcctaat ctacatgctg gcggtagtgg tcaggttgca gcttttaagt
    12901 tgatgacatc accagttgag gttgaagaaa ttgtggcaca tgctggaatt gatattggag
    12961 atacttctat tggaatgcat atcaagagag tacaagttcc cttgattcca attagtcgtg
    13021 agttgggagg agcacacgtt acagcactag ctagtcgtcc taaattaatt ggcggtgcta
    13081 gagcaggata tacgagcgat ccaatccgta aattttaaaa taaacctctg ctctgcagag
    13141 gtttattatt tatctcaacg agagtggtta ttggagaaat tgtacgttgg taagtcaata
    13201 ccttcttcaa gaatagcttt ttgataaagt ttgtaaaatt cagcatagat gtcaaactga
    13261 gcaccgtttt gcgtgaagat attaatacga taaacaaatt gaccattagc attggttgta
    13321 ggaccaaaaa cagtaggctc tccaacgata gcagggtgtt ttgaaacata ctcttcattg
    13381 atttttgtta caatatcaga aatctgatca aggtttgtat gaacaaataa aggaatatca
    13441 atttgagcac gcatattgct acgagattta ttgctgacaa ttgtaatatt acgattagga
    13501 atgaagtgta aggttccatc gaaatcatga atttgtgtgg tgcgtatccc aacattagta
    13561 acagtgcctg atacagtacc aacattaata atatcaccaa catcaaattg gttttctagt
    13621 agaataaaaa agccattgac aacatctgat aagaagcctt gagcaccaag tccaatagca
    13681 accccagcaa taccagcccc agctaataaa ctagaaattg gaacgcccaa tatacttaaa
    13741 atccaataga atagaaagaa ataaagggta tagtttaaaa cattgtgaga aagtttagct
    13801 aatgtttttt gacgagctac tttttgtcta gaaaaggcga gtgagcgatt aacagtcttt
    13861 tcgaaaagat aattgatgac ttgcctaacg ataacgaatg caatgatcaa taataagatt
    13921 gatattagtt tggtgaagaa agtaaataaa acttcctcta cattaagatg gtctataaat
    13981 ttttctaaag taatcatagt aatagtctag taaaaaaaaa gccatagagc aagtatttta
    14041 aaagcttatc gttaagcagt ttaaagcttc ttttttgcat aaaaactttg taaaacgttt
    14101 taaattatgg taaactaaac tgtatctaaa taattttaag gagaaatgac ttaatgtcta
    14161 catcatttga aaataaagct acaaaccgtg gtattatcac atttacaatt agtcaagatg
    14221 agatcaatcc agctcttgat caagcattca acaaagttaa aaaagatttg aacgttccag
    14281 gattccgtaa aggacatatg cctcgtacag ttttcaacca aaaatttggt gaagaagcac
    14341 tctacgaaaa tgcattgaat ttagtgttgc caaaggctta cgaagcagct gttgcagaac
    14401 ttggtttaga cgttgttgct caaccaaaaa ttgatgttgt atcaatggaa aaaggtcaag
    14461 attggaagtt gacagctgaa gttgttacaa aaccagaagt taaacttgga gattacaaag
    14521 acctttcagt agaagttgat gcttcaaaag aagtttcagc tgaagaagtt gatgctaaag
    14581 ttgaacgtga gcgtaacaac cttgcagaat tgactgttaa agatggtgaa gcagcacaag
    14641 gtgatacagt tgttattgac tttgtaggtt cagttgatgg tgttgaattt gatggtggta
    14701 aaggagataa cttctcactt gaacttggtt caggtcaatt tatcccagga tttgaagaac
    14761 aattagtagg ttctaaagct ggtcaaactg tagacgttaa tgtaacattc ccagaagatt
    14821 atcaagcaga agatcttgca ggtaaagatg ctaaatttgt tacaacaatt catgaagtaa
    14881 aaactaaaga agttccagca cttgatgatg aacttgcaaa agatattgat gatgaagttg
    14941 aaacacttga tgagttgaaa gcaaaatatc gcaaagagct agaatcagca aaagaaattg
    15001 catttgatga tgcagttgaa ggtgcagcaa tcgaattagc tgttgccaat gcagaaattg
    15061 ttgaattacc agaagaaatg gttcatgatg aagtacatcg tgctatgaat gagttcatgg
    15121 gtaatatgca acgtcaagga atttcaccag aaatgtactt ccaattaaca ggtacaactg
    15181 aagaagacct tcataagcaa taccaagcag atgctgacaa acgcgttaaa actaaccttg
    15241 ttattgaagc aattgcagct gcagaaggct ttgaagcaac agatgaagaa atcgaaaaag
    15301 aaatcacaga tcttgcttca gaatacaata tggaagctga ccaagtacgc ggtctacttt
    15361 cagcagatat gcttaaacat gatattgcaa tgaaaaaagc ggttgacgtt atcactagct
    15421 cagctactgt aaaataattg cttattaaat tttgaaaaga ttgggcttcc cagtcttttt
    15481 atttgtgatt attcttttga cgaatagcta aaaatagcgt aaaatagtaa atagcgaaaa
    15541 tttgaaaata tgacttatct attttctatc tataaagaat attgctaaac aaaatgatag
    15601 tcttacacaa ttaaggagaa taactttgga attagaagta tttgctgggc aagaaaaaag
    15661 tgaactttcg atgattgaag ttgctcgcgc tattttagag caacgtggtc gtgataacga
    15721 aatgtacttc agcgatttgg taaatgatat tcaaacctac cttggaaaat ctgactcagc
    15781 aattcgtgag tcattgccat ttttctattc agatttgaat acagacggaa gttttattcc
    15841 acttggcgaa aacaaatggg gacttcgttc atggtatgct atcgatgaaa ttgacgagga
    15901 aatcatcact cttgaagaag atgaagatgg cgctccaaaa cgtaagaaga aacgtgtcaa
    15961 tgctttcatg gatggtgatg aagatgctat tgattacaat gatgatgatc cagaagatga
    16021 agatttcaca gaagaaacac caagtttaga atatgatgaa gaaaaccctg acgatgaaaa
    16081 atcagaggta gaatcgtatg attctgagat taatgaaatc attcctgatg aagatttaga
    16141 tgaggatgta gaaatcaacg aagaagacga tgaagaggaa gaagaggaag aagaggttta
    16201 gacagggatt ttgtatttga caaatagtca gatatagttt aaactattat tcgggcacct
    16261 cttatgaggt cagataaagc tccctagtga tagggggcta ttttgttttt gggaaagata
    16321 cagaatggtt ccagatagtt ttctgagtag gcccttgatc agatagtttc tactagtttt
    16381 gactagagaa gctggtatta tttatcaatc atgtgttttg ggttttaccc gtaaactaac
    16441 tgttagatta acatttaatt taaggagttt ttaatgacaa agtatatttt tgttacaggt
    16501 ggtgtggtct cttctattgg taaggggatt gttgcagcaa gtcttgggcg cttactaaaa
    16561 aaccgtgggc taaaggtgac aattcaaaaa tttgatccat atattaatat tgacccaggg
    16621 acaatgagcc cttatcaaca tggagaagtt tatgttacag atgatggtgc agaaactgac
    16681 cttgaccttg gacactacga gcgttttatt gatattaact taaacaaata ttcaaatgtt
    16741 acaacgggta agatttatag tgaagttctt aaaaaagaac gccgtggaga ataccttggt
    16801 gcaactgttc aagttatccc acacgttacg gatgctttga aagaaaaaat caaacgtgct
    16861 gcgacaacta cagattcaga tgttattatt actgaagttg gcggtactgt tggagatatt
    16921 gagagccttc cgtttttaga agcacttcgt caaatgaagg cagatgttgg ttctgataat
    16981 gttatgtaca ttcatacaac gttattacca tacctaaagg ctgccggcga aatgaaaaca
    17041 aaaccgactc aacattctgt taaagagtta cgtggcctag gtatccaacc taatatgctt
    17101 gttatccgta cagagcaacc agcaggtcaa agtattaaga ataaattggc acaattttgt
    17161 gatgttgcac cagaggctgt tatagagtcc cttgatgttg accatattta tcaaattcca
    17221 cttaacatgc aagctcaaaa tatggatcaa attgtttgtg atcacttgaa gcttgagaca
    17281 cctgctgctg atatgacaga atggtcagca atggttgata aagtaatgaa ccttgagaaa
    17341 aaagttaaaa ttgcattggt tggtaaatat gttgaattac ctgatgccta cctttcagtt
    17401 gtggaagccc ttaagcactc agggtacgtc aacgatgttg ctattgattt aaaatgggta
    17461 aatgctgctg aagtgacaga agataatatt aaagaacttg tcggagatgc agatggtatt
    17521 atcgttcctg gtggatttgg ccaaagaggt agtgagggta aaattgaagc aattcgctat
    17581 gcacgtgaaa atgatgttcc aatgcttggt gtttgtctcg gaatgcagct aacttgtgtc
    17641 gaatttgcgc gtaatgtttt aaaacttcat ggagctaatt cagcagagct tgaccctaaa
    17701 acaccgttcc caatcattga tattatgcgt gatcaaatcg atattgaaga tatgggtggt
    17761 actcttcgcc ttggtctata tccatgtaaa ttgaaagcag gttcaagagc tgcggcagct
    17821 tataataatc aagaagttgt ccaacgtcgt caccgtcacc gttatgaatt taatacaaaa
    17881 ttccgtgagc aatttgaggc tgctggattt gtcttttcag gggtatcacc ggataatcgt
    17941 ttgatggaag ttgttgagtt accagagaaa aaattctttg ttgcagcaca ataccatcca
    18001 gaattacaat cacgtcctaa ccatgctgaa gagctttaca cagcttttgt aacagcagca
    18061 gttgaaaata tgaaataact ttatcgcctc ccagtattag gaggcgattt tattttttta
    18121 gatagtttca ctatcaaagt atcattagct cttttctttt tctaaaaata aggtataata
    18181 ggttgcatga aaaagattcg attatcaaag tttattaaaa tgattgttgt tattttgttt
    18241 ttaattagtg tagcagctag tttttatttt ttccacgttg cccaagttcg agatgataaa
    18301 tcctttattt caaatggtca acgtaagcct ggaaactctt tatatgctta tgataaatcc
    18361 tttgataagc tattaaagca aaaaatagaa atgacaaacc aaaatataaa gcaagttgct
    18421 tggtatgttc ctgctgctaa gaaaactcat aagacagctg ttgtcgttca tggttttgcg
    18481 aatagcaaag agaatatgaa ggcatatggt tggctgtttc ataagttagg atacaatgtt
    18541 cttatgcctg acaatattgc acatggtgaa agtcatgggc agttgatagg ctatggctgg
    18601 aacgaccgcg agaacattat caaatggaca gaaatgatag ttgataagaa tccatcaagc
    18661 caaattactt tatttggtgt ttcaatgggt ggagcaacag tcatgatggc tagtggtgaa
    18721 aaattaccta gtcaggttgt taatatcatt gaagattgcg gttattctag tgtttgggat
    18781 gaattaaaat ttcaggctaa agagatgtat ggtttaccag ccttcccact cttatatgaa
    18841 gtttcaacaa tttctaaaat cagagcaggt ttttcgtatg gacaagcaag tagtgtcgaa
    18901 caattgaaaa agaataattt accagccctc tttattcatg gtgataagga taattttgtt
    18961 ccaacaagta tggtttatga caactataaa gctacagcag gtaagaaaga gctttatatt
    19021 gtaaaagggg caaaacatgc gaaatctttt gaaacagagc cagaaaaata tgagaaacgt
    19081 atctctagtt ttttgaaaaa atatgaaaaa taatattgac aacaatatga taagttgata
    19141 taatttatta tgttgttaag cggggatggc ggaattggca gacgcgcaag actaaggatc
    19201 ttgtgaccgc ttttggtcgt gagggttcaa gtccctctct ccgcagacta gtcgagttca
    19261 tttggaactc gactttttgc atatttaaga taaagatgga gtatcacatg tcaaaagtac
    19321 gtggctttga attagttagc caatttagca ataaagagtt attaccaaaa cgagaaacag
    19381 ctcacgcagc tggctatgat ttaaaagtag ctaaaaaaac ggttattgaa cctggtgaaa
    19441 ttacattggt cccgaccggt attaaagcac acatgcaacc aggggaggtc ctgtaccttt
    19501 atgatcgttc atcgaatcca cgtaaaaaag ggattgtcct aataaattca gttggtgtta
    19561 ttgatggtga ctactataat aaccaagtca atgaaggaca tatttttgca cagatgcaaa
    19621 atattactga tcaggctgtt attttagaag aaggggaacg gattgttcag gctgtttttg
    19681 ccccattctt attggcagat gacgatcagg ctacaggaat gcgtacaggt ggttttggta
    19741 gtactggaaa ataaaattat tttacgaata atgttagaaa aggctagact tacttcttag
    19801 ccttttttga tgagagtaaa cttatatttt ctctaacttg gtgtgatata atgagtgtac
    19861 tagttatttg gtaccaagct atcatattaa tttagaagtt ataaaggagg cctgagtggc
    19921 taagaaaaaa tcagtcttca cctgtcaaga gtgtggttac caatcaccta agtatttggg
    19981 acgttgtcca aattgctcag cctggtcttc atttgtagaa gaggttgaag ttcaagaagt
    20041 taaaaatgca cgagttagtt tgaacggtga aaagtcccga ccgacgaagt taaaagatgt
    20101 ttcctcaatt aactattcta gaactaagac tgatatggat gaatttaacc gtgttctagg
    20161 aggcggcgtt gtaccaggaa gtttggttct gattggagga gaccctggga taggtaaatc
    20221 tactttatta ctacaagtat caactcagct agctaataaa ggtacagtat tatatgtctc
    20281 aggtgaggaa tctgcagaac agattaagtt acgaagtgag cgtctaggcg atatcgataa
    20341 tgaattttac ctttatgccg agactaatat gcaaagtatt cggtcggaga ttgagaagat
    20401 taaaccagat tttcttatta ttgattctat acaaactatt atgagtcccg aagtatcaag
    20461 tgtccaaggc tctgttagcc aagttcgcga agtgaccgct gagttgatgc agttagctaa
    20521 aactaataat attgctactt ttattgtagg tcatgtgact aaggagggaa cattagcagg
    20581 ccctcgaatg ctagaacata tggtggatac ggttctttat tttgaagggg agcgtcatca
    20641 cacgttccgt attttacggg cagtcaaaaa ccgttttggt tcgaccaatg aaataggtat
    20701 ctttgaaatg cagtcgggtg gtctagttga agttttgaat ccaagtcaag tttttcttga
    20761 agaacgttta gacggtgcaa caggatctgc tattgttgtt actatggaag gaacaaggcc
    20821 tattttagca gaggttcaag ctttagtgac tccaactgtt tttggaaatg ctaagcgcac
    20881 aaccacaggt cttgacttta atcgtgtcag tcttattatg gcagttctag aaaaacgttg
    20941 tggtttattg cttcaaaatc aagatgccta tttaaaatca gcgggtggtg ttaaattgga
    21001 tgaaccagca attgatttag ctgttgctgt agctattgct tccagttaca aagaaaaacc
    21061 cacaaatcca aaagaatcat ttattggaga aattggactc actggtgaga ttcgtcgcgt
    21121 gacacgtatt gagcaacgta ttaatgaggc tagtaaattg ggctttacta aaatttacgc
    21181 accaaagaat tctctagctg ggattgaaat acctaaaggc attgatgtta ttggtgtgac
    21241 aactgttagt caggtcttaa aagcagtgtt ttcaacataa tgattagatt cggttcagcc
    21301 gaatcttttt tggtggcgag agacatttcg tgaaaaataa tgggcaaaat gataatataa
    21361 aagggttggt atttagtaaa tatcgaatga tgttacacta acaaaggaga tctctatgac
    21421 cacttatttt gataactttt taaaaaccaa tcaagcctat gctgacttac atggaacagc
    21481 tcatctacct atcaagccaa agacaaaggt tgctattgta acctgtatgg attctcgttt
    21541 acacgttgct caagctctag ggctagattt aggagatgct catattttac gtaatgctgg
    21601 tggacgagtt acagacgatg ttctacgttc tttagttatt tcgcaacaac aattaggaac
    21661 acgagaaatc gttgttctac accatacaga ttgtggagca cagacgttta ctaacgaagc
    21721 ttttgctact caattacaga gagatttggg tgttgatatg catggacatg attttcttcc
    21781 ttttaatgat attgaagaaa gcgttcgtga ggatgtggca aaattacatg cttctccatt
    21841 aatcccaaat gatgttgtta tttctggtgc tatttatgat gtagatactg gtcgtatggt
    21901 ggaagttaaa tagaaaaaat cctgtcctta gcgatgggtt tttttatttt agaaaactag
    21961 atttaaaggt aacagttagg cttatcatgc cttatgctgg aattcagaca gaggaaacat
    22021 cttttgctat actgaaacta tattataaaa gcggaggaaa tttaaaatga aaaaatatga
    22081 tgtcattgtt cttggttttg gtaaagcagg taaaacatta gcagcaaaat tagcaacaca
    22141 aggtaaaagc gttgcaatgg tcgaagaaga cgataaaatg tatgggggaa catgtatcaa
    22201 cattggatgt attccgacaa aaacgctttt agtatcagct agcaaaaatc acgattttca
    22261 agaagctatg actactagaa atgaagttac aagtcgttta cgtgctaaaa attttgctat
    22321 gttggataat aaagacactg tggatgttta taatgctaaa gctcgcttta tatcaaataa
    22381 agtggtcgaa ttaacaggag gagctgacaa acaagaactt acagctgatg ttattattat
    22441 caatactggt gctaagtcag tacaattacc aatcccagga ttagctgatt ctcaacatgt
    22501 ttatgattca acggcaatcc aagaattggc tcatcttcct aaacggttag gtatcattgg
    22561 aggcggtaat attggtcttg aatttgcgac tttatattct gaattaggga gcaaagtgac
    22621 agttattgat agtcaatctc gtatctttgc gcgtgaggaa gaggaattat cagagatggc
    22681 tcaagattat ttagaagaga tgggaatatc ttttaaatta tcagccgata tcaaatctgt
    22741 gcaaaatgaa gatgaagacg ttgttattag ctttgaggat gaaaaattaa gctttgatgc
    22801 agtattatat gcaacaggac gtaagccaaa tacagaaggt ttagcacttg aaaatacaga
    22861 tattaaattg acagaacgtg gagctattgc tgttgatgaa tattgccaaa cttctgttga
    22921 gaatattttt gcagttggtg atgttaatgg cggccctcaa ttcacatata tttctttaga
    22981 tgactcacgt attgtcttaa attatttgaa tggtgataag gattattctc taaaaaatcg
    23041 aggagcagta ccgacttcaa cctttacaaa tcctccttta gcaacggtag gtctagatga
    23101 aaagaccgct aaggaaaaag gatatcaagt taaatctaat agccttcttg tttctgccat
    23161 gccacgtgca catgttaata atgaccttag aggtattttt aaagttgtgg tagatactga
    23221 gacaaatctt attttgggag ctcgcttatt tggtgcagaa tcccatgaat taattaatat
    23281 catcacaatg gcaatggata ataaaatccc atatacttat ttccaaaaac aaattttcac
    23341 tcacccaaca atggtagaga attttaatga tttgtttaac ttttaagaat atttttaaaa
    23401 agtaaggcaa ttttgcttta ctttttttat cgaaaattag tgtggctatt attactgatt
    23461 atcattttta gtgatttagt ggtataatag ggttgattgg caatcatctg taagagtttg
    23521 aatgatgttt tgcccccttg actaattcag ttaaactgaa aaagtttact atcagaaaat
    23581 ataggagtag aattttggct aataagattc gtgtgcgtta tgcaccaagt ccaacaggat
    23641 tgttacacat cggaaatgcg cgtactgcgc tctttaacta cctttatgct cgccatcatg
    23701 gtggggattt tgttattcgt attgaagata ctgaccgaaa acgccacgtc gaagatggtg
    23761 aacgctctca attggagaac ttacgttggc taggtatgga ctgggatgaa agccctgaaa
    23821 cacatgaaaa ttatcgtcaa tcagaacgcc ttgagctcta ccaacgttat attgatcagt
    23881 tgttagcaga aggtaaagcc tacaagtcct atgtgactga ggaagaatta gcagcagagc
    23941 gtgaacgcca agaattggca ggcgaaacac ctcgctatat caacgaattt atcggaatgt
    24001 cagaaactga aaaagaagct tacattgcag aacgtgaagc atcaggcatc attccgacag
    24061 tacgccttgc tgttaatgaa tctgggattt ataaatggac tgatatggta aaaggtgaca
    24121 ttgagtttga aggctctaat atcggcggag actgggttat tcagaagaaa gatggttatc
    24181 caacttacaa tttcgcagta gttattgatg atcatgacat gcaaatttcg catgttattc
    24241 gtggcgatga ccatattgca aatacgccaa aacaattaat ggtttatgaa gcactcggtt
    24301 gggaagcgcc acaatttggt cacatgacat tgattataaa ctcagagact ggtaagaaat
    24361 tatcaaaacg cgacaccaat actcttcaat ttatcgaaga ctatcgtaaa aaaggttata
    24421 tgtcggaagc tgtctttaat ttcattgccc ttcttggttg gaatcctggt ggagaagaag
    24481 aaatcttctc acgcgagcaa ttaattaatc tatttgatga aaatcgtctt agcaaatcac
    24541 cagcagcatt cgatcagaag aagatggatt ggatgagtaa tgattaccta aaaaatgctg
    24601 attttgagag tgtttttgca ctttgcaaac cattcttaga agaagctggt cgacttacag
    24661 acaaagctga aaaattagtt gagctttata aacctcaatt aaaatcagct gatgagattg
    24721 taccattaac agaccttttc tttgcagatt ttccagaatt aacagaagcc gaaaaagaag
    24781 taatggcagc agaaacagtc ccaacagtct tatctgcttt taaggaaaag ttagtatcgc
    24841 tttctgacga agaattcact agagatacta ttttccctca aattaaggct gttcaaaaag
    24901 aaacaggtat taaaggtaag aacctattta tgcctattcg tattgcggtt tctggtgaaa
    24961 tgcatggacc agagttacca gatactattt atttattagg taaagaaaaa tcagttcaac
    25021 atatcgataa tatgttagca aaattataat ataaaaagtc gtgaattttc acgacttttt
    25081 gtgtactttt accaattgta tttttcaaca ttgtctttgg taacaaggta aattggagaa
    25141 attgtttctt tctctacttt tttgccttta taatgatcaa ttgctgcttg aattgctatt
    25201 tctcccatct tagctggttg ttgtgcaata gtggctgaaa tatctccttt tttaatggca
    25261 tcatgggcat ctggctgacc atcaattcct acgataagaa cattttgcaa gccagcagat
    25321 ttgacagcct gtgcagcccc aagtgccatt tcatcatttt gtgcgaaaat aatttggaca
    25381 tctttatgtc cttgaatcat attttgtgta gtattcaaag ctttggcgcg gtcaaaattg
    25441 gcagattggc ttgaaagaat gtcaagttta gattttgcaa ctgaatgaaa tcctttacca
    25501 cggtctacag tagcagaggc acctggtact ccagataatt caaaggcttt tgcttttttg
    25561 cctagttttt taacagcata atccgcagcc atcttaccag cagcaacgtt atctgaagcg
    25621 acagtagtta atactttgcc tccctcacta ccacggtcca ttagaataac aggaatatta
    25681 gcgttgtttg cagacttaat agctgttaca atagcttttg agtcaacggg attaatcaaa
    25741 atagcatcta cgttttgact aataaagttt tggacatcat ctgcttgacg tgcggcgtca
    25801 tcttgagcat cagctacctt aatgcttatt tttttattgc tagcatattt gtcaataccg
    25861 tctttcatcg caacaaagta gggattgtta gttgttgaaa tagatacacc tagtttcaag
    25921 tttttagcag attttttggt tacatttttt gtagaattcc cagtagaatt tccaagtcca
    25981 gttttaccac atgcacctag aatgaggaca atagacatca ataaggctag aaagccaagt
    26041 ttttttccaa atttcatttg aaactccttt agataaaata taatgaattt atagaaatta
    26101 gagtaaagac tattttgcta ctttaaagcg atcaagtaaa acagccatta aaataacgat
    26161 acctttcaca acttgttgcc aaaatgcaga gacaccaatg atgtttaatc cattgttcag
    26221 gacaccaata atgagagcac caatcaatgt tccaatgata cgtcctttcc caccagataa
    26281 agacgttcca cctaagacaa cggcggcaat agcatccatc tcataacttg ctccagctgt
    26341 tggttgtgct gagctaaggc gtgatgtgat aattaaccca gaaatggaag ccatgatacc
    26401 tgagatagtg tagatgataa ttttaacttt gtttaactta atacccgaga tatacgctgc
    26461 tttttcatta cctcctaaag cgtaaacaga tttcccgaag gctgttttat gtaacaaaat
    26521 atacaaaata ataaaagtaa gaaacatgag aatgactggg aatgggatac caacaatata
    26581 gccttgtccc aaaaattgga atagaaaact atcggataag ccagcggtaa ttgggttgcc
    26641 gtttgaataa actagcgttg ctcctctaaa gatagtcatt gttgccagtg taacgataaa
    26701 tggtgctaat tttccgtaag aaatgaagag tccgtttagc attcccaaga tgccacctaa
    26761 ggcaacagat aaaacgatag caataccaac gggtactccg tggccgatta atccagctgt
    26821 taatgcacta gatagggcta atatggaccc gactgataaa tcaataccgc cagtcaaaat
    26881 aacgaaggtc atcccgaaag cgataaaacc atttgcagtt acttgtaata ataaattgag
    26941 tagattattg gttgtgagga aattaggatt aatgagtgtg ataataacca ttaaaccagc
    27001 taacgcaatc agggttgtta actctgatat atatttcatc atatttttca ctttacttac
    27061 ctcctgtggc tagttgcata actttttctt ggtcagcttc ttttcgactg agttctccac
    27121 tgattcgacc ttcgtgcatt accatgatgc ggtcactaac acctagaatt tcaggtaagt
    27181 ctgatgatac catgacaatt ggaacacctc tgtctgctag ttcattcatt aattggtaaa
    27241 tctctcgttt agctccgaca tcaacaccac gtgttggctc atcaagaatt aagactttag
    27301 gagcaatacc aatccattta gcgagaacga ccttttgttg gttacctcca gaaagatttc
    27361 caacctctaa atctggtctc cctgatttaa tgtagaggcg attaatcaat tgttgcacaa
    27421 atgttgtgct tgttttttca tcaaagaaac catgtttgct aaaatcttta gtgctaggga
    27481 gagtcatatt gtccttgata ttgaagtcta ggatgagacc ttcatctttt ctattttcgg
    27541 tcaaaaaacc aatgccctgc ttgatagctt gacttgggct agtaatagtg atttcttggt
    27601 tatcaatttt caccttacca gacttctttt tatcaattcc aaagatagtt cgcataactt
    27661 cagtacgccc agctcccatt aatccagaaa atccaagaat ctcgccttta cggacataaa
    27721 aggaaacgtc ctcgaaatta tctccacaga ggttactaac ttcaaaagca acaggcccaa
    27781 tttcagaatg tttttcagga tagtaatctt ccaattttcg gccaaccatt tttttaacca
    27841 attcgtcaga atttgtgagt gatgtttctt ttgtatctac aatgacacca tctctcatga
    27901 cggttacgaa gtcagtgatt ttaaaaattt cctccattcg atgggagata tagacaatgc
    27961 ccactccttc ttgttttaaa cttcggataa ctcggaaaag attttcagtt tcacgatctg
    28021 ttaaagcagc agtcggttca tccattacca aaatagagag ttggtttagg agacttttgg
    28081 cgatttcaat catttgttgt tgtccaacag acaagttacc aatcgggatg tctaggggaa
    28141 tagtcacacc taatctttta aaagcttcaa gtgctttttg tctcattaat ttttgattga
    28201 gtaagccaaa agttgtcttg atttcacgtc caagaaagag attttccaag acagtcattt
    28261 ccggccaagt attcatctct tggtgaataa aactaatgcc aaattcctct gcttcttgag
    28321 ggtttgaaaa agttcgttcc tcgccatcaa tataaatagt tcctgtactt gctggaaaaa
    28381 gtcctgttag aatgttcatt aaggttgatt tacccgcacc attttcaccc ataagcgcgt
    28441 gtatttgacc agattgtaat tccaaatcaa tcttctctag aactttatta gtaccgaagg
    28501 atttggagat attcctcatg tcaattttca tggactaaac tcctttctag atgataacac
    28561 cagattgtag aataatattt gaatacggtg tattttcacc tgtacgaata actgctttga
    28621 catcttgggt catttgtttt aaatgatcgt gactgacgta ctctatgcta acagagttat
    28681 caagtttagc taataacctt gataattgat ccggatttgc ttccttgatt tcttctgcta
    28741 aaatgacttt ttcaactaag atattctcta aatagatatc aagtacctct tggaaactag
    28801 gtattccaga ggtcaaagac aaatcaatct ttggaatgcc atttggaaca ggtagaccca
    28861 gatctccaat acaaactcga tcagtatgcc caaggtcatc tgctaattta gcgagatgac
    28921 tatttaatat gccagtcttt ttcataatgt tggttgtcct ccatttcttt aattgtaggc
    28981 atgccaccct gtgcaccaaa tttttgcacg gataagtgag cagctagagt tgcaaaacgt
    29041 aaggcattag caatcttaaa ttttttcgag atagcataac cgaatgcacc gttaaaagta
    29101 tcacctgcac cagttgtatc aacagcttta gtttctaagg caggaattaa atggctttct
    29161 tttccatcag agtagatggc tccttttgtg ccgagtgtta caattagttt ttcaggatat
    29221 gtggctaata tatcctctag tttttggttt gggaacagct cttgacattc gtgttcattt
    29281 ggtgtgaaat aatcgacctt atcgagcatc tcaatatcag tctttctaga tggtgcagga
    29341 ttatagagta atttaatact atgttcttta caaaaattag caattttcat atttgcttga
    29401 tgaggaattt cattttgtaa tacaactaaa tcagcttctt taattattga ccactcctga
    29461 gaccactttt tagtatcaac ttttccgttg gctccaggac agtaaatgat tcgattatca
    29521 ttattataaa gagtgatttg ggcaacacct gaagaagatg gtatcgttcc cacaaaatcc
    29581 gtagtaacat ggtttttgtt taaattatcc aaaagaatgg gaccaaaaga atcctcgcca
    29641 atggcaccaa gaatagtaat attatctcga tcttggctaa gacgcccaat agcgacagcc
    29701 tgattagcac ctttacctcc tggaaccatt gaaaaacgtt ggccaaatac ggtttctccc
    29761 tctttggcga tacgattggt ttccataact aagtccatgg aaatactacc gataatgaca
    29821 atgttactca tctattttcc tccttagggt ttcacgttcg acataatgaa cgggtaattt
    29881 aatacgctta ttggtaatag gtaattgatt agtaattttg aaaattaatt cagctgcttt
    29941 ttgtcccata atatatgatg attggtgaat agttgacaac gaagggtaga taaattggct
    30001 cattaaaata tcatcataac caataatttg tacatcttca gggattcgct tccctcgatt
    30061 aagaatctcg tgtaggtagg caatagcatg tatatcagat ggagcgataa tgctatcaat
    30121 atctggatgg tggtcaagta aagttttcgc ttcaatttga atctctgcaa aatcaaaact
    30181 ggcactatct tcgatagcaa aacgagcccc tttgtttaat aggtaatttt ggcttccttg
    30241 aaacctttga ttaaggttat cagctttatc tagcggacca cgaatgagta atatattggt
    30301 agcacctgct gtccaaatag cttgagctgc taattttcct ccctctttat tatcagagaa
    30361 aacgccgtac tgtgtttctt gattaacgcg gtcaacaact acaactggaa tatctatttc
    30421 aggatgattt ttggtgaaat cgtgtgttgt aataatccct gcagcattac tttgtaatag
    30481 cacatttaag tactcttctt ctaaatggct cttgttattt gtgttgccaa gcatgacacg
    30541 ataaccctgt tcttttagaa attcttcaac tcctctagca agtcttggaa agaaagggtt
    30601 tgaaatatcg ggaagtagta atccaacaag ttgatttttt ttggttttca gagattgtgc
    30661 aaggaaattt ggaacataat ggagttcgcg gatagcttgc tcgatttttt gacttgcttt
    30721 ttgactaacg taaccatttt gggatatata ccttgagaca gtagatgttg atacgccagc
    30781 tttctcggcg acttgtctaa tcgtactcat agaaactcct ttcttttatt gtgggaacgg
    30841 tttcataatt agaattgtaa tcgcttacta aaaaattgtc aagctatttt ttaaataatt
    30901 taatgataaa atatcattat ttttagaggt catattcaag tcataaagct gtgatactat
    30961 gttcacatca aaagatcaaa ggagaaaaac catgtcacat gcttggaaag aattaacctt
    31021 ttataagaaa aaatacctcc tgattgaatt gctaatcatt gttatgatgt tcatggtagt
    31081 ttttttatca ggtttagcaa acggtttagg tagagctgtt agtgctgcta ttgaaaataa
    31141 cccagctcaa acctatattt tgaatgaagg tgctgaacaa gtgattacat catctgtatt
    31201 gacaactaaa gatcagacag atttaaacag tttgaattta aaagatagta ccactttaaa
    31261 tattcaacgt tcgagtttaa ctcgacaagg acatgaaaag aaaattgata tttcttattt
    31321 tgctattgat aaagattctt ttatggcacc tacactttct gagggaaaac aactgacaag
    31381 ttacaaaaaa gctattattt taaatgatag tttaaaagct gaagggataa agttaggtga
    31441 taaagtgatt gataagtcat catctatatc actaacagtc gttggatttg tccataatag
    31501 tatgtatggt cacggacctg tggcatttat tgataaagac atttataccg aaattaataa
    31561 aaagattaat cctcaatacc agttcttacc tcaggcatta gttatgaaaa atgataaaag
    31621 tatatctcat ttaccaactc aattagaagc tgttagtaaa aaagatgtca ttcaacatat
    31681 tcctggttat agtgccgaac aatccacttt gaatatgatt ttatgggtcc ttgtcgtagc
    31741 ttcagctggt attttaggtg tctttttcta catcatcaca cttcaaaagc gtcatgaatt
    31801 tagcgtcatg aaagctattg ggacaaaaat gtcagagatt gccctatttc aattgagtca
    31861 agtcatcata ttggcactgt ttggtatcat tgttggagat ggattggcga ttgctctctc
    31921 ttatgtatta ccagctcaaa tgccatttgt tattaattgg caaaatatta ttttagtatc
    31981 gtttgtattt ttagtcattg caatgattag tagtgctctg tcaattgtaa aagttgctaa
    32041 aatcgaccca gtcgaagtta ttaatggggg aggagaataa tgtcagcaat cttagaatta
    32101 aaacacatct caaaacacta tccagatgga gatgagcttt tatcaatatt agataattta
    32161 gatttatcag tttctgcagg tgagtttgta gctatactgg gaccctcagg ttcaggtaaa
    32221 agcacacttt tatccattgc agggttactt cttggcgcag atcaaggtag tttgtatgtc
    32281 aatcatgaga acgttacgga tttgtctcaa agacaaagaa cacaattgcg ccgtgaagca
    32341 ctaggtttta tatttcaaag tcatcaactg cttccttatt taacaatcca agaacagttg
    32401 caacaagagg ctcgatttgc aaaacactat gataagaaaa caagcttgga agaaattaat
    32461 aaattattat cagatttagg aatagagcag tgtgcacata aataccctaa ccaattatcg
    32521 ggaggacaaa agcaacgcgc agcaattgct agagcattta ttaatcatcc taaggttatc
    32581 ttggcagatg agccaacagc tagccttgat gaagaacgtg ggcgtcaagt tacagaactt
    32641 attcgtcagg aagttaaatc acataatact gcagctatta tggtgacaca cgatgagcgc
    32701 gttctagatt tagtagatac cgtttatcgt ttaaaggacg ggaaattagt taaagaaaac
    32761 ttctaacttg tgttacaata agctagaggt gatttaatga ttaaaatatt agttgtagag
    32821 gataacattg ttcaacaaaa aatcattacc acaaaattaa cacaagaggg ctaccaattt
    32881 atcactgctt caaatggaca agaagctcta aattgtctag atacagaaga agtacaattg
    32941 attataactg atatcatgat gcccatgatg gatggttatc aattaattca agaactcagg
    33001 tcagctgctt ataatgtccc aatcattgtt atgacagcaa aatcacaaat ggaagatatg
    33061 acaaaagggt ttggccttgg tgcagatgat tatatggtta agcctgtgca gttacaagaa
    33121 ttagctttac gtattaaggc actcttgagg agagctaata ttgtagctca acatcagcta
    33181 attattggga atacttgtct taatgaagat gaattaagct tgaaatattt tgagcaagaa
    33241 attgtatttc ctcagaaaga atttagggtt cttttccact tacttagcta tcctaatagg
    33301 attttcacac gtttagaact attggatagt atttggggaa tggatactga tttagatgag
    33361 cgggtggtag atgcttgcat caataaaatt agacgaaaag tagaacactt accagatttt
    33421 aagattgaga cggtgagagg ggtagggtat cgtgcaaaaa atgactaagc taaggcgatt
    33481 tcggtttccg ttaagatttt atttcacatt aatgtttgtg ttaacgatgc ttttctcagt
    33541 cctagcttct cttttactag ttgcagcaat agtttttact ttttttcaag gagtcttaac
    33601 tactcatgta ttacaagtta gcgctctcgc tgttgttttt ttaagccttg tgatagctag
    33661 tattagtatg tggtacggtt cttatcattt aacaaaacca attttagata ttagccgtat
    33721 tgtgtccaat gttgcagacg gtgactttga agggcatatt tatcgaaaca gtaatcgtcg
    33781 taaatcctat gagtattata atgaattgga tgagctatct gaaagtatta atcagatgat
    33841 tgtttcttta tctcatatgg atcatatgcg aaaagacttt atcactaatg tttctcatga
    33901 gctaaagact ccgattgctg cagtagctaa tattgtagaa ctattacaag atccagaatt
    33961 agatgaagaa acacaaagtg agttattagg tcttgttaaa acagagagcc taaggttaac
    34021 aagattatgt gatacgatgt tgcaaatgtc gcgcgtggat aatcaggaga ctattgggga
    34081 gctatcctct gttcgtgtag atgaacaaat tcgtcaagca atgattagtt taacagaacg
    34141 ttggcaagct aaaagaataa attttcaact ggattcaaaa ccatatactg tttactcaaa
    34201 ctctgactta ctgatgcaag tttggataaa ccttttagat aatgcaataa aatattccga
    34261 tgatattgta gacttacgtg tacgaatgga agaaactaat aatcattatc tgagagttat
    34321 tatttctgat aaaggtaggg gaatttctca atatgatgtt cagcatatat ttgataaatt
    34381 ttaccaagcg gatcagtcgc ataatcaaca aggaaatgga ttaggactag caatagttaa
    34441 acgtataatt gttctttgta agggacgtat tagtgtctcc agtcagtttg aaattggaac
    34501 agaattttgt gtagaactac ctttatcata attatttaaa aca
//
LOCUS       DBJYSH010000005        33818 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.005, whole genome shotgun sequence.
ACCESSION   DBJYSH010000005 DBJYSH010000000
VERSION     DBJYSH010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 33818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33818
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.005"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            complement(26..1261)
                     /locus_tag="ACUBV3_000215"
     CDS             complement(26..1261)
                     /locus_tag="ACUBV3_000215"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyltransferase"
                     /protein_id="HHU3846752.1"
                     /translation="MALKELTAKEFESYSGNYDLQSFMQTPEMAKLLKKRGYDITYMG
                     YQIDGKMEIISIVYTIPMTGGLHMEVNSGPAHSNSKYLKHFYKELQNYAKSQGTLELL
                     IKPYDTYQEFTGEGEPKGAPNTYLIDDLTSIGYHHDGLHIGYPGGEPDWHYVKNLKGI
                     TPQNLLKSFSKKGRPLVKKAMSFGIKIRVLKREELHIFKDITSSTSDRRDYMDKSLDY
                     YQDFYDSFGDKAEFVIATLNFREYDHNLQLNAKKLEEQIAVLDNRHQNNTDSAKYHRQ
                     RTELVNQLASLDKRRKEVEPFIQKFGNQDVVLAGSLFIYSPKETVYLFSGSYTEFNKF
                     YAPAVLQEYVMQEALKRQSTFYNFLGIQGNFDGSDGVLRFKQNFNGYIVRKMGTFRYY
                     PNPLKYKSIQLLKKILRRT"
     gene            complement(1280..2491)
                     /locus_tag="ACUBV3_000216"
     CDS             complement(1280..2491)
                     /locus_tag="ACUBV3_000216"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyltransferase"
                     /protein_id="HHU3846753.1"
                     /translation="MYREITAVEHDRFVSESNQTNLLQSSNWPKVKDNWGSQLLGFFD
                     GETQIASASILIKSLPLGFSMLYIPRGPIMDYSNLDIVTKVLKDLKAFGKKQRALFIK
                     CDPLIYLKMVNAKDFENSPDEKEGLIAIDHLQRAGADWTGRTTDLAHTIQPRFQANLY
                     ANQFGLDKMSKKTRQAIRTSKNKGVDIQFGSHELLEDFAELMKKTEDRKGINLRGIDY
                     YQKLLDTYPNNSYITMASLDVAKRLEKIEKECQIAQSERIKSLELNREKKVKQHQDTI
                     DRLNKEIDFLKEAQKAYDRDIVPLAATLTLEFGNTSENIYAGMDDYFKSYSAPIYTWF
                     ETAQRAFERGNIWQNMGGIENDLSGGLYHFKSKFEPIIEEFIGEFNIPVNRLLYKASN
                     YVYALRKKRNS"
     gene            complement(2504..3724)
                     /locus_tag="ACUBV3_000217"
     CDS             complement(2504..3724)
                     /locus_tag="ACUBV3_000217"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009854600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyltransferase"
                     /protein_id="HHU3846754.1"
                     /translation="MYHVTVGISEKEYDAFAIASSQTNLLHSSKWAQVKSNWQNERLG
                     FYKGDQLVAVASILIKSLPLGFTMLYIPRGPIMDYSNKELVGFVLKTLKNFGRKKRAV
                     FAKFDPALLLRQYHLKEENVAEEIDESRQAIDNLKSAGAQWIGPTKAISETIQPRFQA
                     NIYTKANIEENFPKHTKRLIKDAKHRGVQIYRANIDDLPKFATVVALTENRKGVALRN
                     ENYFHQLMTIYGEDAYLYLAKVNLPKRLAQFKEQLLQIQKDLSETPSHQKSRLTRLNQ
                     QEASVKQYILEFQEFSKKYPDEPVIAGILSIRFGNVLEMLYAGMDDSFRKFYPQYLLN
                     ARVFEDAFKNGIASANLGGVEGSLNDGLTKFKSNFNPMFEEYIGEFNLAINPLLYKLA
                     NLAYTIRKKQRHSH"
     gene            complement(3724..4536)
                     /gene="yidA"
                     /locus_tag="ACUBV3_000218"
     CDS             complement(3724..4536)
                     /gene="yidA"
                     /locus_tag="ACUBV3_000218"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HHU3846755.1"
                     /translation="MTSIKLVAVDIDGTLLNSKREITPEVAKAVQEAKSKGVKIVIAT
                     GRPIIGVQDLLEELKLNEEGDYVITFNGGLVQDTATGDDIIKETLTYEDYLDFELLAR
                     KLGVHMHAITKEGIYTANRDIGKYTIHEATLVNMPLFYRTPEEMGDKEIIKLMMIDQP
                     DILDAAIAKIPKKVLDNYTIVKSTPFYLEILPKNVNKGTALLHLAEKMGLTVDQTMAI
                     GDEENDRAMLEVVGNPVVMQNGNPELKKIAKYITKSNEESGVAYALREWVIN"
     gene            complement(4607..5923)
                     /locus_tag="ACUBV3_000219"
     CDS             complement(4607..5923)
                     /locus_tag="ACUBV3_000219"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HHU3846756.1"
                     /translation="MNEKVFRDPVHTYIHVNNQVIYDLINTKEFQRLRRIKQTSTTSF
                     TFHGAEHSRFSHCLGVYELARKVTEIFDEHYPDLWNKNESLLTMAAALLHDIGHGAYS
                     HTFERLFNTDHEAYTQEIITNPTTEINAILRKVAPDFPDKVASVINHSYPNKQVVQLI
                     SSQIDCDRMDYLLRDSYYTAASYGQFDLTRILRVIRPTDSGIAFARNGMHAVEDYIVS
                     RFQMYMQVYFHPASRAMELLLQNLLKRARFLFDTHRDFFEQTSPNLIPFFTDQYDLQD
                     YLALDDGVMNTYFQSWMQADDNILADLANRFINRKVFKSITFEEADKENLVKMKELVS
                     QVGFDPDYYTGVHANFDLPYDVYRPEHSNPRTEIQIIQKNGQLAELSSLSPIVKALTG
                     SNYGDQRFYFPKEMLTLDSLFSSTKEEFQSYITNEHLTLTKDDSHS"
     gene            5999..6385
                     /locus_tag="ACUBV3_000220"
     CDS             5999..6385
                     /locus_tag="ACUBV3_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000764119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HHU3846757.1"
                     /translation="MKLEINNNIQIDNETEMIHEIHDCQFIEKGSYVYLNYINAEGER
                     VVIKANHEELLMTRFSSPKSVMRFHRETPALVNIPTPLGVQHLITETSHYQFDLSQQR
                     LHIDYVLKQTETGDCFANYELRIQWY"
     gene            6729..9413
                     /locus_tag="ACUBV3_000221"
     CDS             6729..9413
                     /locus_tag="ACUBV3_000221"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005388 - P-type calcium transporter
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HHU3846758.1"
                     /translation="MLKEQKKSLFYTQGQEEVLTSLESSREGLSTTEAKNRLEMYGRN
                     ELEEGKKRSLIAKFFDQFKDLMIIILLVAAALSVITEGMHGLTDALIILVVVILNAAF
                     GVYQEGQAEAAIEALKDMSSPIARVRRDGHTIEVDSKELVPGDLVMLEAGDVVPADLR
                     LLEAASLKIEEAALTGESVPVEKDISQVVAEDAGIGDRVNMAYQNSNVTYGRGYGVVT
                     NTGMYTEVGKIADMLANADESETPLKQSLVQLSKLLTYLIVIIAVITFLVGIFVRKEG
                     WIEGLMTSVALAVAAIPEGLPAIVTIVLSMGTKTLAKRNSIVRKLPAVETLGSTEIIA
                     SDKTGTLTMNQMTVEKVYTNGVLQSSSEEISVDNNTLRIMNFSNDTKIDPSGKLIGDP
                     TETALVQFGLDKNFDVREVLKNEPRVAELPFDSDRKLMSTIHKESDGRYFIAVKGAPD
                     QLLKRVTKIEDNGLVRDITAEDKEAILNTNKELAKQALRVLMMAYKYETQIPTLETDI
                     VESDLVFSGLVGMIDPERPEAAEAVRVAKEAGIRPIMITGDHQDTAEAIAKRLGIIDA
                     NDTEDHVFTGAELNELSDEEFQKVFKQYSVYARVSPEHKVRIVKAWQNDGKVVAMTGD
                     GVNDAPSLKTADIGIGMGITGTEVSKGASDMVLADDNFATIIVAVEEGRKVFSNIQKS
                     IQYLLSANMAEVFTIFFATLLGWDVLAPVHLLWINLVTDTLPAIALGVEPAEPGVMTH
                     KPRGRQSNFFDGGVMGAIIYQGILQTILVLGVYGWALMYPEHAGYRMIHADALTMAFA
                     TLGLIQLVHAFNVKSVYQSIFTVGAFKNRTFNWSIPVAFILLMVTIVVPGFNKLFHVT
                     HLSSTQWLTVVIGSLLMVVLTEIVKFIQRKLGQDEKAI"
     gene            complement(9458..10318)
                     /locus_tag="ACUBV3_000222"
     CDS             complement(9458..10318)
                     /locus_tag="ACUBV3_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HHU3846759.1"
                     /translation="MTKLAIMSDLHIDLNHFSTFETKTLINLLKKEKIDHLHIAGDIS
                     NHFTKDTLPFINNLKKHIKLSYNLGNHDMLDLTETEIQRLDFQTYQFDKKMLLAFHGW
                     YDYSFSNNRDIKDVEKLKKTFWFDRRLKRPNNDVTIQASILKRLDEILAKVDSSNIII
                     AMHFVPHKQFTMTHPRFSPFNAFLGSQAYHDLFQKYHIKDVVFGHAHRSFGDVKIGET
                     TYHSRPLGYIREWNLTIDFVNQNPNHNPNLTWNLSKRYNAVKHLDSFENYRKKYFEDE
                     LRNSMTIFDC"
     gene            10470..12401
                     /locus_tag="ACUBV3_000223"
     CDS             10470..12401
                     /locus_tag="ACUBV3_000223"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HHU3846760.1"
                     /translation="MSNFYKLLKEKFPRKEDIVTEMINLEAICQLPKGTEYFISDLHG
                     EYDAVDYLLRTGAGSIRAKLLDCFDWQKIVAVDLDDFCILLYYPKQKLAFDKMNLSAS
                     AYKTKLWEMIPLQIQVLKYFSSKYTKSKVRKQLPGKFAYIIEELLAEIDRNPEKKSYF
                     DTIIEKLFELDQVEDLIIVLSQTIQVLIIDHLHVVGDIYDRGRYPDRILNRLMAFPNL
                     DIQWGNHDVTWMGAASGSYLCMVNVIRIAARYNNITLIEDRYGINLRRLVDYSRRYYE
                     PLPSFAPILDGEEMTHPDELDLLNMIQQATAILQFKLEAQLIDRRPEFQMHNRQLINQ
                     VNYKDLSISIKGVVHQLKDFNSRCIDSKNPSRLTSEEEELLQQLMIAFQTSESLKKHI
                     DFLFEKGSMYLTYNDNLLFHGCIPMHSNGDFKSFKIAGKTYGGRDLLDLFESQIRLAY
                     ARPEKHDDLATDIIWYLWCGENSSLFGKNAMTTFERYYVSDKVTHQERKNPYFKLRDK
                     DDICTTLLQEFDLPKFGHIVNGHTPVKEKNGEQPIKANGKMLVIDGGFAKGYQKNTGL
                     AGYTLIYNSYGIQLISHLPFTSIEEVLSGTNYIIDTKRLVEEAKDRILVKDTTIGQKL
                     TKEIKDLDHLYRHFQEYDD"
     gene            12491..13615
                     /gene="queG"
                     /locus_tag="ACUBV3_000224"
     CDS             12491..13615
                     /gene="queG"
                     /locus_tag="ACUBV3_000224"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HHU3846761.1"
                     /translation="MDIKAEIQKLAKEIGISKIGFTTADNFDYLEKSLRASVEEGRNS
                     GFEHKVIEDRIYPERLLESAKTIISIGVAYPHKLPQQPQKTPYKRGKITPNSWGLDYH
                     YVVGEKLDRLSKGIEELCRDFPLQQKAMVDTGALVDTAVAQRAGIGFIGKNGLVISKE
                     YGSYMFLGELITNLEIEPDKLVDYDCGDCRRCLDACPTSCLIGDGSMNAKRCLSFQTQ
                     DKGMMDIEFRKKIKTVIYGCDICQICCPYNKGINNPLATEIDPELAQPELIPFLSLSN
                     GQFKEKFGMIAGSWRGKNILQRNAIIALANAHDKTAVVKLIEIIDKNNNPIHTATAIW
                     ALGEIVKKPNDEILEFMSNLTLKDEDSRKELELIRHKWQF"
     gene            13684..14782
                     /gene="prfB"
                     /locus_tag="ACUBV3_000225"
     CDS             join(13684..13755,13757..14782)
                     /gene="prfB"
                     /locus_tag="ACUBV3_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889043.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HHU3846762.1"
                     /translation="MEVAEIRQKIVENKEKLTSFRRSLDLDRLEEEIALLENQMTEPD
                     FWNDNIAAQKTSQELNELKGKYDTFHNMQELSDETELLLEMLDEDDSLKEELEENLMQ
                     LDKIMGAYEMTLLLSEPYDHNNAILEIHPGSGGTEAQDWGDLLLRMYTRFGNANGFKV
                     EVLDYQAGDEAGIKSVTLSFEGPNAYGLLKSEMGVHRLVRISPFDSAKRRHTSFASVE
                     VMPELDDTIEVEVRDDDIKMDTFRSGGAGGQNVNKVSTGVRLTHIPTGIVVSSTVDRT
                     QYGNRDRAMKMLQAKLYQLEQEKKAQEVDALKGDKKEITWGSQIRSYVFTPYTMVKDH
                     RTNFELAQVDKVMDGEINGFIDAYLKWRIED"
     gene            14801..15493
                     /gene="ftsE"
                     /locus_tag="ACUBV3_000226"
     CDS             14801..15493
                     /gene="ftsE"
                     /locus_tag="ACUBV3_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HHU3846763.1"
                     /translation="MALIEMSGVTKKYRRSTTALRNLNLSIQQGEFVYLVGPSGAGKS
                     SLIRLLYREEKLSSGRLKVGEFNLNKLKRRQIPILRRSIGVVFQDYKLLPTKTVYENV
                     AFAMQVIGAKRRHIKKRVPEVLELVGLKHKMRSFPTQLSGGEQQRVAIARAIVNNPKL
                     LIADEPTGNLDPEIAWEIMHLLERINLQGTTVLMATHNSQIVNTLRHRVIEIEAGSVI
                     RDEEKGEYGYHD"
     gene            15477..16406
                     /gene="ftsX"
                     /locus_tag="ACUBV3_000227"
     CDS             15477..16406
                     /gene="ftsX"
                     /locus_tag="ACUBV3_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HHU3846764.1"
                     /translation="MVIMINFFRHFWESLKNLKRNFWMTFASVTSVTITLLLVGLFSS
                     VLLNVEKLTTDVSGNFTISAFLNVDSTDAQKQVKDKDGKLKDNPDYHKVYDKIKRISG
                     VEKVTYSSKAEQLKEVQKEYGSDVIDDTYKDALLDVYVVGTSSAKVSKNVSEAIGRIE
                     GVDYTKEPIDSTKLSNLTDNIRIWGFGGVALLIVLAIFLISNTIRMSIMSRRTDIEIM
                     RLVGAKNSYIRGPFFFEGAWVGILGAIVPSLIFYFGYQFVFNKFNPKFETSHVSLYPM
                     DIMVPAIIGGMVIIGIIIGSLGSVLSMRRYLKI"
     gene            complement(16459..17169)
                     /locus_tag="ACUBV3_000228"
     CDS             complement(16459..17169)
                     /locus_tag="ACUBV3_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001022568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HHU3846765.1"
                     /translation="MNIKRHSIFLWLTTSILAILLAIGMTIYFNTYQASPLARRIAKT
                     ATNHKNYLLFRSKGKVKANIIFYQGALVEEEAYSQLARDLADKGYNTYILKTPLNLPV
                     LSPYKAKTIIDQNHLTNVYLAGHSLGGVVASQNAKVAPVRGLILLASYPSSKSDLSHK
                     KLRVLSITASNDHILNWEKYEEAKKRLPNSSTFRTIVGGNHSRFGNYGHQKGDGKATL
                     SHKSSEKQLATFISNFIH"
     gene            complement(17166..17801)
                     /locus_tag="ACUBV3_000229"
     CDS             complement(17166..17801)
                     /locus_tag="ACUBV3_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000708399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HHU3846766.1"
                     /translation="MKIYKTINHIAGENTYYLVNDQAVILIDPGSNGQEIIAKIKSFE
                     KPLVAILLTHTHYDHIFSLDLVRDTFDNPPVYVSEKEAAWLSSPDDNLSGLGRHDDII
                     NVIARPAENFFKLKQPYQLNGFEFTVLPTPGHSWGGVSFVFHSDELVVTGDALFRETI
                     GRTDLPTSNFEDLITGIRQELFTLPSHYSVHPGHGMNTTIGHEKNFNPFFQ"
     gene            18032..18796
                     /locus_tag="ACUBV3_000230"
     CDS             18032..18796
                     /locus_tag="ACUBV3_000230"
                     /EC_number="1.1.1.304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019152 - acetoin dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0045150 - acetoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-acetoin forming diacetyl reductase"
                     /protein_id="HHU3846767.1"
                     /translation="MSKVAIVTGAGQGIGFAIAKRLHADGFKIGVLDYNEETAQAAVD
                     KLSPEDAVAVVADVSKRDQVFDAFQKVVDTFGDLNVVVNNAGIAPTTPLDTITEEQFE
                     KAFAINVGGTIWGSQAAQKHFRELGHGGKIINATSQAGCEGNPNLTVYGGTKFAVRGI
                     TQTLAKDLASEGITVNAYAPGIVKTPMMFDIAHEVGKNAGKDDEWGMEQFAKDITLKR
                     LSEPEDVANAVGFLAGDDSNYITGQTIVVDGGMVFH"
     gene            18946..21411
                     /locus_tag="ACUBV3_000231"
     CDS             18946..21411
                     /locus_tag="ACUBV3_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DnaQ family
                     exonuclease/ATP-dependent helicase"
                     /protein_id="HHU3846768.1"
                     /translation="MTQKKLRKYAVVDLEATGAGPNASIIQVGIVIIQGNKIIDSYET
                     DVNPHESLDEHIVHLTGITDKQLVKAPDFGQVAHHIYQLIEDCIFVAHNVKFDANLLA
                     EQLFLEGFELRTPRIDTVELSQVFYPCLEKYSLGALAESLNIELTDAHTAIADARATA
                     QLFIKLKAKISSLPKEVLKTILTFADNLLFESYLLIEEAYQEADFVNPKEYYFWQGLV
                     LKKEKAVGKPKKLSSDFQVNMALLGMDARPKQIVFADLVKAHFNDQTTTFLEAQSGLG
                     KTYGYLLPLLDQSQKQQIIVSVPTKILQDQIMAKEIKHIQELFHIPCHSIKGPRNYLK
                     LDAFYKSLQVQDRNRLINRFKMQLLVWLTETTTGDLDEIKQKQRLESYFDQLKHDGEV
                     TQSSLFYDLDFWKRSYDKVAQSQLVIINHAYFLERVQDDKDFAKGKVLVFDEAQKLVL
                     GLENFSRGQLDISHQLQVIQKIIDSPIPLLQKRLLESISYELSHAAELFYRHNSFEFS
                     ETWLKRLKNSINALEVVGLDDLQTFFTATYTNYWFETDKVNEKRLTILRGAREDFLKF
                     SEFLPPTKKTYMISATLQISPKVYLSDLLGGFSSISTEKIAHEKNANQKVWIDTSMPN
                     ILDLSPEQYAYEIAKRLQDIMTLKQPTLVLLTSKQTMFMVSDYLDKWEIKHLTQDKNG
                     LAYNVKKRFDRGESNLLLGTGSFWEGVDFVHRDRLIEVITRLPFDNPKDYFIQKLSQS
                     LTKEGKNFFYDYSLPMTVLKLKQALGRTTRREEQKSAVIILDSRLVIKSYGQTIMHSL
                     GRDFEISKEKINKVLTEMAKFLI"
     gene            21497..22690
                     /locus_tag="ACUBV3_000232"
     CDS             21497..22690
                     /locus_tag="ACUBV3_000232"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HHU3846769.1"
                     /translation="MTYLSERVLNMEESVTLAAGAKARELRVQGRDILSLTLGEPDFA
                     TPKNIQQAAIEAITDGRASFYTPSSGLPELKSAINAYFERFYGYSLKPNQVVVGTGAK
                     FILYTFFMTVLNPGDEVIIPTPYWVSYADQIKMAEGKPVFVTAKEVNHFKVTVEQLEA
                     VRTDKTKVILLNSPSNPTGMIYKAEELEAIGNWAVEHDILILADDIYGRLVYNGNIFT
                     PISSLSESIRNQTIVINGVSKTYAMTGWRVGFAVGNHDIIAAMSKVVSQTTSNLTAVS
                     QYATIEALNGSQESFEKMRLAFEERLNIIYPLLCQVPGFEVVKPQGAFYLFPNVTKAM
                     EMKGYTDVTAFTDAILEEVGLALVTGAGFGAPENVRLSYATDLETLKEAVRRLHVFMG
                     SNEIN"
     gene            22711..24057
                     /gene="asnS"
                     /locus_tag="ACUBV3_000233"
     CDS             22711..24057
                     /gene="asnS"
                     /locus_tag="ACUBV3_000233"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HHU3846770.1"
                     /translation="MSKKLISIVDVKDYVGQEVTIGAWVANKSGKGKIAFVQLRDGSA
                     FFQGVAFKPNFIEKYGEESGLEKFDVIKRLNQETSVYVTGIVKEDERSKFGYELDITD
                     LEVIGESHEYPITPKEHGTDFLMDNRHLWLRSRKQMAVMQIRNAIIYSTYEFFDQNGF
                     IKFDSPILSENAAEDSTELFETDYFGKPAFLSQSGQLYLEAGAMALGRVFDFGPVFRA
                     EKSKTRRHLTEFWMMDAEYSFLSHEESLDLQEAYVKALIQGVLDRAPQALDILERDVE
                     ALKRYIAEPFKRVSYDDAITLLQEHEADEDTDYEHLEHGDDFGSPHETWISNYFGVPT
                     FVVNYPASFKAFYMKPVPGNPERVLCADLLAPEGYGEIIGGSMREDDYDALVAKMDEL
                     GMDKSEYDFYLDLRKYGSVPHGGFGIGIERMVTFVAGTKHIREAIPFPRMLHRIKP"
     gene            complement(24097..24654)
                     /locus_tag="ACUBV3_000234"
     CDS             complement(24097..24654)
                     /locus_tag="ACUBV3_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HHU3846771.1"
                     /translation="MTKSPARLISFISIAIAINLVGANLALFLRLPIYLDTIGTLLIA
                     VILGPWYAASTAFLSALINWMTTDIFSLYYSPVAIVVAIITGILIKRNCKPSSLLWKS
                     LIISLPGTIIASVITVILFKGITSSGSSIIAQFLHGIGLDMTSSLILVQVGTDYMDRL
                     ISLILVFSTITLLKKHSPNLINQRF"
     gene            complement(24651..25634)
                     /locus_tag="ACUBV3_000235"
     CDS             complement(24651..25634)
                     /locus_tag="ACUBV3_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001031103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HHU3846772.1"
                     /translation="MNKEKIIIDCDPGIDDTLALMYAIQHPKLEVVAITITAGNSPVE
                     LGLKNTFVTLELLNRHDIPVYVGDNLPLQREFVSAQDTHGMDGLGENNFTLAQPIIFQ
                     EESADCFLANYFEHKNDTSIIALGPLTNIARALQTNPKLGKHCKRFISMGGSFKSHGN
                     CSPVAEYNYWCDPHAAQYVFENLDKKIEMVGLDITRHIVLTPNHLSYMERINPDVSSF
                     IQKITKFYFDFHWQYEHIIGCVINDPLAIAYFVNENIATGFDSYTDVACHGIAMGQTI
                     VDQYHFYKKDANSKILTSVNTNLFWNDFMTVLLHAQNSVIIEDLEMLGLNK"
     regulatory      complement(25778..25822)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            complement(25880..26293)
                     /locus_tag="ACUBV3_000236"
     CDS             complement(25880..26293)
                     /locus_tag="ACUBV3_000236"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HHU3846773.1"
                     /translation="MLYEVTSSNTQDVDGKVYLSNGKIVETNHPLNHLPGFNPEELIA
                     LAWSTCLNATIKAILEQKGFKDLKSRVDVTCQLMKEKQVGKGFYFQVNAVASIEKLSL
                     SDSKLIVNKAHSRCPISKLISNAKTINLDTVKWSV"
     gene            26447..27337
                     /gene="rapZ"
                     /locus_tag="ACUBV3_000237"
     CDS             26447..27337
                     /gene="rapZ"
                     /locus_tag="ACUBV3_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HHU3846774.1"
                     /translation="MSDEQIKLVIVTGMSGAGKTVAIQSFEDLGYFTIDNMPPTLVPK
                     FLELAAQSGDTSKIAMVVDMRSRLFFREINSILDSLEINDNINFKILFLDATDTELVS
                     RYKETRRSHPLAADGRVLDGISLERELLAPLKSMSQNVVDTSELTPRQLRKVISEEFS
                     NQDSQSSFRIEVMSFGFKYGIPLDADLVFDVRFLPNPYYKPELRDKTGLDTEVYDYVM
                     SFDESDDFYDHLLALIKPILPGYQNEGKSVLTVAIGCTGGQHRSTAFAHRLSEDLKAD
                     WTVNESHRDKNKRKETVNRS"
     gene            27334..28308
                     /locus_tag="ACUBV3_000238"
     CDS             27334..28308
                     /locus_tag="ACUBV3_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001231103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HHU3846775.1"
                     /translation="MRKPKITVIGGGTGIPVILKSLRLEDVEITAVVTVADDGGSSGE
                     LRSVMQLTPPGDLRNVLVALSDMPKFYEQIFQYRFAEGDGDFAGHPLGNLIIAGVAEM
                     QGSTYNAMQSLTQFFHTTGKIYPSSEHPLTLHAVFKDGHEVVGESQIADYKGMIDHVY
                     VTNTYNEETPTASRKVVDAILESDMIVLGPGSLFTSILPNLVIPEIKQALLETRAEVA
                     YVCNIMTQRGETEHFTDADHVEVLKRHLGQDAIDTVLVNIEKVPESYMENNHFDEYLV
                     QVEHDFSGLRKHARRVISSNFLKLEKGGAFHHGDFVVEELMNLVGTHL"
     gene            28305..29216
                     /gene="whiA"
                     /locus_tag="ACUBV3_000239"
     CDS             28305..29216
                     /gene="whiA"
                     /locus_tag="ACUBV3_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000011317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HHU3846776.1"
                     /translation="MSFTVKVKEELLGHKSENKMELSAIIKMSGSLGLANHGLNLSIT
                     TENAKIARHIYSMLEEHYHLQPEIKYHQKTNLRKNRVYTVFIEEKVDVILADLKLADA
                     FFGIETGIEHSILDNDENGRAYLRGAFLSTGTVREPDSGKYQLEIFSVYLDHAQDLAN
                     LMKKFMLDAKVIEHKHGAVTYLQKAEDIMDFLIVIDAMEARDAFEEIKMIRETRNDIN
                     RANNVETANIARTITASMKTINNIIKIMDTIGFDALPSDLRQVAQVRVAHPDYSIQQI
                     ADSLETPLSKSGVNHRLRKINKIADEL"
     gene            29231..30628
                     /locus_tag="ACUBV3_000240"
     CDS             29231..30628
                     /locus_tag="ACUBV3_000240"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HHU3846777.1"
                     /translation="MACTTILVGKKASYDGSTMIARTEDSVNGDFTPKKLKVMTSKDQ
                     PRHYKSVLSNFEVDLPDNPLPYTSVPDALGKDGIWGEAGINSKNVAMSATETITTNSR
                     VLGADPLVSDGIGEEDILTLVLPYIQSAREGVERLGAILEKYGTYESNGIAFSDTEEI
                     WWLETIGGHHWIARRVPDDVYVTNPNQLGIDHFEFNNCDDYMCSSDLKEFIEQYHLDL
                     TYSNEHFNPRYAFGSQRDKDRHYNTPRSWAMQRFLNPEIEQDPRSLFIPWCQKPYRKI
                     TVEDIKYVLSDHYQDSVYDPYGPEGDAVSRRAFRSVGINRTSQTSILQLRPNKSLETT
                     GVQWLSYGSMPFATMVPLFTQVETVPDYFSNTTKDASTDNFYWTNRLIAALADPHFYQ
                     HEADIESYIERTMAQGHAHINGIDREVAENKEIDFQQKNQEMSDYIQKESQELLNRIL
                     FDASNLMTNRFSMGD"
     gene            30770..32296
                     /locus_tag="ACUBV3_000241"
     CDS             30770..32296
                     /locus_tag="ACUBV3_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HHU3846778.1"
                     /translation="MRKKFLLLMSFVAMFAAWQLVQVKQVWADSKLKVVTTFYPVYEF
                     TKNVVGDKADVSMLIKAGTEPHDFEPSTKNIAAIQDSNAFVYMDDNMETWAPKVAKSV
                     KSKKVTTIKGTGDMLLTKGVEEEGEDHEHEGHGHEGHHHELDPHVWLSPERAISVVEN
                     IRNKFVKAYPKDAASFNKNADAYIAKLKDLDKEYKNGLSNAKQKSFVTQHAAFGYMAL
                     DYGLNQVPIAGLTPDAEPSSKRLGELAKYIKKYNINYIYFEENASNKVAKTLADEVGV
                     KTAVLSPLEGLSKKEMAAGEDYFSVMRRNLKVLKKTTDVAGKEVAPEEDKTKTVETGY
                     FKTKDVKDRKLTDYSGNWQSVYPLLQDGTLDPVWDYKAKSKKDMTAAEYKKYYTAGYK
                     TDVESIKIDGKKHQMTFVRNGKSQTFTYKYAGYKILTYKKGNRGVRYLFEAKEKDAGQ
                     FKYIQFSDHGIKPNKAEHFHIFWGSESQEKLFEEMENWPTYFPAKMSGREVAQDLMSH
                     "
     gene            complement(32409..32669)
                     /locus_tag="ACUBV3_000242"
     CDS             complement(32409..32669)
                     /locus_tag="ACUBV3_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018370011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HHU3846779.1"
                     /translation="MKKDIHPDYRPVVFLDTTTGYKFLSGSTKSTKETVEFEGETYPL
                     IRVEISSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAQ"
     gene            complement(32778..33713)
                     /locus_tag="ACUBV3_000243"
     CDS             complement(32778..33713)
                     /locus_tag="ACUBV3_000243"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000583237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HHU3846780.1"
                     /translation="MIIFQQILDKIKEYDTIIIHRHMRPDPDALGSQIGLRDIIRHNF
                     PKKKVLATGFDEPTLTWIAKMDQVTDQDYQGALVIVTDTANTPRIDDERYKKGDFLIK
                     IDHHPNDEIYGDLSYVDTNASSASEIVTDFALSCDLLLSTSAARVLYNGIVGDTGRFL
                     YPATTSKTLKIASKLREFDFDFSAMARQMDSFPFKIAKLQGFIFEQLKIDKNGAACVT
                     LTQEDLKRFDVTDAETAAIVGVPGKIDIVESWAIFVEQSDGHYRVRLRSKSHIINEIA
                     KRHNGGGHPLASGANSYSLEENQAIYQEIQEVLSL"
BASE COUNT        10849 a         5413 c         6691 g        10865 t
ORIGIN      
        1 aaataagctt atgtaagctt attttttatg ttcggcgtaa gattttcttt agtaattgaa
       61 tactcttata ttttaatgga tttggatagt agcggaaagt acccatttta cgaacaatgt
      121 aaccattaaa attctgttta aagcgaagta cgccatctga accatcaaaa tttccttgaa
      181 tacctaaaaa attatagaaa gttgattgac gctttaaagc ctcttgcata acatattctt
      241 gaagaactgc aggcgcataa aatttattaa attctgtgta tgagccagag aaaagataga
      301 cagtttcttt agggctataa ataaagaggc taccagctag aacaacatct tgatttccaa
      361 atttttgaat aaaaggttcg acttctttgc gacgtttatc taaactagct agttgattga
      421 caagctcagt acgttgacga tggtatttgg cagaatctgt attattttga tggcgattat
      481 caaggactgc aatctgttct tccaatttct ttgcatttaa ttgtaggtta tgatcatatt
      541 ccctaaaatt tagagttgca atgacaaatt ccgccttatc tccgaaacta tcataaaaat
      601 cttgataata atctagagat ttatccatat agtcccggcg atcagaagta gaagaagtaa
      661 tatccttaaa gatatgtagt tcttcccgct tgaggacacg tatttttata ccaaaagaca
      721 tggctttctt aaccaagggg cgtccttttt tagagaaaga tttaagaaga ttttgaggag
      781 tgataccttt tagattttta acataatgcc aatcaggttc accgccagga taaccaatat
      841 gaagcccgtc gtggtgataa cctatactag tcaggtcatc aataagataa gtgttcggtg
      901 cacctttagg ctcaccttca ccagtaaact cttggtaggt atcataaggt ttaattaata
      961 attctaaagt gccctgagat ttggcatagt tctgtagttc tttataaaaa tgtttcagat
     1021 atttagaatt ggaatgggct ggaccagaat tgacttccat atgaagtcca ccagtcattg
     1081 ggatggtata aacaatactg atgatttcca ttttcccatc gatttggtag cccatgtagg
     1141 taatgtcata cccacgtttt tttagaagct ttgccatctc aggggtttgc ataaacgatt
     1201 gtaaatcgta atttcctgaa taactttcga actctttagc tgttaattct ttaagcgcca
     1261 taaaagtatc ctttcctaac tagctattac gttttttacg taaggcatag acataattag
     1321 aggctttata aagcaatctg ttcactggaa tgttaaattc tccaataaat tcctcaatga
     1381 ttggttcgaa cttagactta aaatgatata aaccaccaga taagtcattc tcaatccccc
     1441 ccatattttg ccaaatattt ccacgttcaa aagccctttg agctgtttca aaccaagtgt
     1501 aaataggagc agaataagat ttgaagtaat cgtccatacc agcataaata ttttctgatg
     1561 tattaccaaa ttctagagtg agggttgcag ccaatggaac aatgtctcgg tcataggctt
     1621 tttgagcttc tttgagaaaa tcaatttctt tatttaatcg atcaatcgta tcttggtgtt
     1681 gtttaacttt tttctcacga ttaagttcaa gtgattttat tctttcagat tgagctatct
     1741 gacattcttt ttctattttt tctaatcgtt ttgcaacatc tagcgaagcc attgtgatgt
     1801 atgagttatt aggatacgta tcaaggagtt tttggtagta gtcaattcct ctaagattta
     1861 tccctttacg gtcttccgtt tttttcatta actctgcaaa atcttcaagt agttcatggc
     1921 taccaaattg tatatctact cccttatttt tggaagttcg aatagcttga cgagtttttt
     1981 tagacatttt atcaagtcca aattgattag catataggtt tgcctgaaaa cgtggttgga
     2041 tagtatgagc taaatctgtt gtccgaccag tccaatcagc acctgcacgc tgtaaatgat
     2101 caatggcgat taacccttct ttttcatcgg gcgaattttc aaaatctttg gcattgacca
     2161 tttttaaata gatgagagga tcacacttga taaagagagc tctttgtttt ttgccaaaag
     2221 ctttaaggtc cttaaggacc ttagttacaa tatctagatt ggagtaatcc atgattggtc
     2281 cacgcggaat atatagcatg gagaagccaa gaggaagtga tttgatgaga atactagcgc
     2341 tggcaatttg ggtttcaccg tcaaaaaagc caagtaattg actaccccag ttgtctttta
     2401 ctttgggcca attagaagat tgaagtaggt ttgtttggtt ggattcgctc acaaagcgat
     2461 cgtgttcgac agcggtaatt tctctataca tgagtttcct ttcttagtga gagtgtcttt
     2521 gtttttttcg aatagtatag gctaggttgg ccaatttata taataatgga ttgattgcta
     2581 gattaaactc gccaatatat tcttcaaaca ttggattgaa gttactttta aattttgtta
     2641 aaccatcatt aagacttccc tcgacaccgc ctagattagc agatgcaata ccgtttttga
     2701 aagcatcttc gaacacccta gcattcaaga gatactgtgg ataaaattta cggaagctat
     2761 catccatacc agcatacaac atctccagga cattgccaaa acgaatggac aaaattcctg
     2821 caatgacagg ctcatctgga tatttcttgc taaactcctg aaattcaagg atatattgtt
     2881 taacagaagc ttcttgttga tttagccttg tcaggcgact tttttgatgg gatggagttt
     2941 ctgataaatc tttttggatt tgaagaagtt gttctttaaa ttgtgctaat cttttaggaa
     3001 gattgacttt agccaaatag agataggcat cttctccata aattgtcatt aattgatgga
     3061 aatagttttc attacgtaaa gctacacctt tacgattctc ggttaatgct acaaccgtgg
     3121 cgaatttagg taaatcatca atgttggcac gataaatttg aacaccgcga tgctttgcat
     3181 cttttatcag acgtttagtg tgttttggaa agttttcttc tatatttgct ttggtataga
     3241 tattcgcttg aaaacgaggt tggatggttt cggaaatggc tttcgttgga cctatccatt
     3301 gggcacctgc tgacttgaga ttatcaattg cctgtctact ttcatctatc tcttcggcga
     3361 cattctcttc tttaaggtga tattgacgta gaagaagtgc tggatcgaat tttgcaaaaa
     3421 cagcacgttt ctttcgtcca aagtttttta gtgtttttaa cacaaagcca actaattctt
     3481 tattgctgta gtccataata ggtccacgtg gaatataaag cattgtaaaa ccaagtggta
     3541 atgatttaat taagatgcta gctacagcaa ctaattggtc acctttataa aaacctaaac
     3601 gttcattttg ccaatttgat tttacttgtg cccactttga agagtggagg agattggttt
     3661 gagacgatgc aatagcaaag gcatcatatt ctttttcgga tattccaacg gtaacatgat
     3721 acatcagttt attacccatt cacgtagtgc gtaggctacg ccactttcct cgtttgattt
     3781 ggtgatatat tttgctattt tttttagttc aggattgccg ttttgcataa caacaggatt
     3841 tccaacaact tcaagcatag cacggtcatt ttcttcatca ccaatagcca ttgtttgatc
     3901 tacagttaat cccattttct ctgcaaggtg gagaagtgca gtgcctttat taacgttttt
     3961 gggtaaaatt tctaaataaa aaggagttga ttttacaatg gtgtagttat cgagaacttt
     4021 ttttggaatt ttagcaattg ctgcatctaa gatgtcaggt tgatcaatca tcattagttt
     4081 aatgatttct ttgtctccca tttcttcagg agtacgatag aagagcggca tattaactaa
     4141 agttgcttcg tgtattgtat atttaccaat atcacgattt gctgtgtaaa ttccttcttt
     4201 tgtaatggcg tgcatgtgga cgcctaattt acgcgctaac agttcgaaat ccagatagtc
     4261 ttcataggtt aaggtttctt tgatgatgtc atctcctgtg gctgtgtctt gaactagacc
     4321 gccgttaaag gtgataacat aatccccctc ttcattgagt ttcaattctt ctaaaagatc
     4381 ttgaactccg atgattggac gtcccgtagc aataacaatt ttaacgcctt ttgattttgc
     4441 ttcttgaaca gctttagcca cttcaggtgt aatttcacgc ttactattta atagggtgcc
     4501 gtcaatatct acggcaacaa gtttaattga agtcatttta tctcccactc tttattcaat
     4561 atcctaaaat actcacctta tatcttacta aaaaataaag ggtttgtcat gaatggctat
     4621 catctttcgt gagtgtcaaa tgctcatttg tgatgtaaga ttgaaattct tcttttgtac
     4681 ttgaaaatag gctatctaat gtcaacattt cttttggaaa ataaaaacgt tgatcaccat
     4741 aattagatcc agttaatgct tttacaatcg gtgataagct tgataattca gcaagttgtc
     4801 cattcttttg gataatttga atttctgttc gtggatttga atgttcaggc cgataaacat
     4861 cgtaaggtaa gtcaaaatta gcatggacac cagtgtaata atcgggatca aaaccaacct
     4921 gtgagactag ttctttcatt ttaactagat tttctttgtc agcctcttca aaggtaattg
     4981 atttaaagac ttttcgattg ataaaacgat tagctaaatc tgccaaaatg ttatcatcag
     5041 cttgcatcca agattggaag taagtattca ttaccccatc atctagggct aaataatctt
     5101 gtaaatcata ttgatccgtg aagaaaggga taagatttgg agaagtttgt tcaaaaaaat
     5161 cgcgatgggt atcaaataag aagcgagcac gttttagtaa attttgtaag agtaattcca
     5221 tagcacgact ggctggatgg aaataaactt gcatatacat ttggaagcga ctaacaatat
     5281 aatcttccac agcgtgcatc ccgttacgag caaaagcaat tccagaatcg gttggtctaa
     5341 taacgcgtaa aattcgtgtt aaatcaaatt gtccatagct agcagcagtg tagtaagaat
     5401 ctctgagaag atagtccatt cgatcacaat caatttgact agaaattaat tgaacaactt
     5461 gtttattggg gtagctatga ttaataacac tagctacttt atcagggaaa tcaggagcca
     5521 cttttcgtaa aatagcatta atctctgtgg taggatttgt gatgatttcc tgagtgtaag
     5581 cttcatgatc agtattgaag agacgctcaa atgtatgaga atatgcgcca tgtccgatat
     5641 catgtagtag ggcggcagcc atcgttagca aggactcatt tttattccaa aggtcagggt
     5701 aatgttcatc aaagatttct gtaaccttac gagccagttc ataaactcca agacaatgtg
     5761 aaaaacgact atgctctgca ccatggaagg taaaagaagt cgttgaggtt tgtttaatgc
     5821 gtcgtaaacg ttgaaattct ttagtgttaa tcaagtcata tatgacttga ttatttacat
     5881 ggatataagt gtgaacgggg tcacgaaata ctttttcatt catatatata ttataacaaa
     5941 aattgaaact tgagtcagaa attggtagaa tagaaggata aaagaagtga gagaagtcat
     6001 gaaactagaa attaacaata acattcaaat tgacaacgaa accgaaatga ttcatgagat
     6061 tcacgattgt caatttattg aaaaaggaag ttacgtttat ttgaattata ttaatgctga
     6121 gggcgagagg gtagttatta aggcaaatca tgaggaattg cttatgacga ggttttcaag
     6181 tcctaagtct gtaatgcgtt ttcatcgtga aacgcctgct ttggttaata tccccactcc
     6241 acttggtgtt caacatttga ttacagagac aagtcactat cagtttgatt tgtcacagca
     6301 aaggttgcat atcgattatg tccttaagca aacggagaca ggagactgtt ttgcaaatta
     6361 tgaattaagg attcaatggt attaagataa ctaccattaa caaaagagta gtcgttatga
     6421 atttcgtcta ctgaagacgc tgggatctct ttatgagagc catttatttg ataaactgca
     6481 gtatggttta tttcgactag atgaatttcg ttttttccct tgagattatt agtgatagca
     6541 aggataatta attgaaaaat ggattttttt ctgttacaat gattaacata tcaagggagt
     6601 agcagacggt ttaccgtggc aatgtcgtca ttacgaaaga aatttccggc gttgtcttaa
     6661 acagcgagac ttgttttcat aaaaacaggt ctctttttgt ttgtggagag accaaaggag
     6721 gaaaggtttt gttaaaagaa cagaaaaaat ccttgtttta cacacaaggt caagaagagg
     6781 ttctaacgtc tttagaatct tctcgtgaag gattatcaac aacagaagcg aaaaatcgct
     6841 tagagatgta tggtcgcaat gagcttgaag aagggaaaaa acgtagtcta atagctaagt
     6901 tttttgacca attcaaagat ttaatgatta ttatcttact ggttgctgca gctctctctg
     6961 ttattacgga aggtatgcat ggcttaacgg atgcattgat tatcttggta gttgttatct
     7021 taaatgcagc ttttggtgtt tatcaagagg ggcaagccga agcagctatt gaagccctca
     7081 aagatatgtc aagcccaatt gcgcgtgttc gtcgtgatgg tcatactatt gaggtggatt
     7141 ctaaagaact agttcctggt gacctcgtta tgttagaagc aggagatgtt gttcccgcag
     7201 atttacgact tttagaagcc gcgtctctaa aaattgaaga agcagctcta actggtgagt
     7261 cagtacctgt tgagaaagac atatctcaag tagtagcaga agatgcaggt attggtgacc
     7321 gtgtgaatat ggcgtatcaa aattcaaatg tcacgtatgg tcgaggatat ggtgttgtta
     7381 ctaacacagg gatgtatact gaagttggta aaattgcgga catgttggca aatgctgatg
     7441 aatctgaaac accattgaaa caaagtttag ttcaactttc taaactattg acttacttaa
     7501 tcgtgattat tgcggttatt actttcttag tggggatatt tgtccgtaaa gaaggttgga
     7561 ttgaagggtt gatgacttca gttgctttag cagttgcagc tattccagaa gggcttcctg
     7621 caattgttac gattgtctta tcaatgggaa caaaaactct tgctaaacgc aattctatcg
     7681 ttcgtaaact ccctgcagtt gaaactttag gttctacaga aatcattgca tcggataaga
     7741 ctggtactct gacaatgaat caaatgactg tcgaaaaagt gtatactaat ggcgttctcc
     7801 aatcatcttc ggaagaaatt tcagttgata ataacacttt acgtattatg aatttctcca
     7861 acgatactaa gattgatcct tcaggaaaat taattggtga cccaacagag actgcactgg
     7921 tacaatttgg tttagataag aattttgatg tccgtgaagt tttgaaaaat gaaccacgtg
     7981 tagctgagtt accatttgat tcagatcgta agttaatgtc tacgattcat aaagagtcag
     8041 atggtcgcta ctttatcgca gtcaagggtg ctcctgatca attattaaaa cgagttacta
     8101 aaattgaaga taatggcttg gttcgtgata tcacggctga agataaagag gctatcctaa
     8161 ataccaataa agaactagca aaacaagctc ttcgtgtctt gatgatggct tataaatatg
     8221 aaacacaaat tccaacttta gagactgata ttgttgagtc agatttagta ttctcaggtt
     8281 tagtgggaat gattgaccct gagcgtccag aagcggcaga agctgttcga gttgctaaag
     8341 aagcaggaat tcgtccgatt atgattaccg gggaccatca agatactgca gaagctattg
     8401 caaagcgtct aggtattatt gatgcaaatg acacagaaga tcatgtcttt acgggtgctg
     8461 aacttaatga attaagtgac gaagagtttc aaaaagtatt taaacaatat tctgtctatg
     8521 cacgtgtttc tcctgaacat aaagtgcgta tcgtaaaagc ttggcaaaac gatggcaaag
     8581 tcgttgccat gactggtgat ggtgttaatg atgctccatc gctaaaaaca gcagatattg
     8641 gtattggtat gggaataact ggtacagaag tttccaaagg tgcttctgat atggttcttg
     8701 ctgatgataa ttttgcaact attattgtgg ctgttgagga aggacgtaaa gtcttctcaa
     8761 atatccaaaa atctattcaa tatcttcttt cagccaacat ggcagaggta tttacaattt
     8821 tctttgcaac gttattagga tgggatgtac ttgcaccggt tcaccttctt tggattaact
     8881 tggtaacaga tactcttcca gctatcgctc ttggagttga gccagctgaa ccaggagtta
     8941 tgacacataa acctcgtggt cgccagtcaa acttctttga tggtggtgtt atgggagcta
     9001 ttatctatca aggtatttta caaactatct tagtacttgg tgtctatggt tgggcattga
     9061 tgtatccaga acatgctggt tatcgcatga ttcacgcaga tgcccttacg atggcctttg
     9121 caacgcttgg tttaatccaa ttggtacatg catttaacgt taaatcagtt taccaatcaa
     9181 tctttacagt tggagctttt aaaaatagaa cctttaactg gtctattcca gtagctttca
     9241 ttcttttgat ggtgactatt gttgttcctg gatttaataa acttttccat gtaactcatt
     9301 taagttcaac acaatggtta actgtagtta tcggaagttt attgatggta gtgttaacgg
     9361 aaattgttaa atttatccaa cgtaaattag gacaagacga aaaagctatt tagtttagct
     9421 gataaaaaaa ctcacttaga atatctaagt gagtttttta gcaatcaaat attgtcatag
     9481 agttgcgtaa ttcatcttca aaatattttt ttcgatagtt ttcaaaacta tccaaatgct
     9541 taacggcatt ataccttttg ctaagattcc aggttaaatt agggttatga ttgggattct
     9601 gattgacaaa gtcaatagtt aaattccatt ctctaatata tccaagtggt ctagaatgat
     9661 aagtagtctc tccaatttta acgtcgccaa atgagcggtg tgcatgacca aaaacaacat
     9721 ctttgatatg atatttttgg aataaatcat gataagcctg actccccaaa aaagcattaa
     9781 aaggagagaa tctaggatga gtcatcgtga attgtttatg gggaacaaag tgcatagcaa
     9841 taatgatatt tgaagaatcg acttttgcta atatttcgtc taagcgtttt aaaattgatg
     9901 cttggatagt tacatcatta tttggacgtt ttaaacgtct atcaaaccaa aaagtctttt
     9961 ttaatttttc aacatccttg atgtctctat tatttgaaaa gctgtaatca taccagccat
    10021 gaaaagctag tagcattttt ttgtcaaatt gataggtttg aaagtcaagg cgttggattt
    10081 ctgtttctgt taaatcaagc atatcatggt ttcctaaatt atagctaagt ttaatatgct
    10141 tttttaagtt attgataaag ggaagtgtat ctttagtaaa atgattagat atatctccag
    10201 cgatatggag gtgatcaatt ttttctttct taagcaaatt aattaacgtt ttcgtttcaa
    10261 aggtagaaaa atgattgagg tcaatatgta aatcactcat gatagcaagt ttggtcataa
    10321 gggtattgta ccatattgta gttcaaagag ttaaacagta aatttttgaa atagttttag
    10381 agtcattcta taatagatat gtcattgtag aatgcgcttt cataagtgta tttagaagct
    10441 ttatatttaa ggtgccttaa ggaggtctca tgtctaattt ttataagctc ttgaaagaaa
    10501 aatttcctcg aaaagaagat atcgtaacag aaatgatcaa tttagaagct atttgtcaat
    10561 tacccaaagg gacggaatat tttattagtg atttacacgg ggaatacgat gctgttgatt
    10621 atctcttacg gacaggcgca gggtctatta gagctaaatt attagactgc tttgattggc
    10681 agaaaatagt tgctgttgat ttagatgatt tttgtatact gctttattac ccaaagcaaa
    10741 aattagcctt cgataaaatg aacttgtcag caagtgctta taaaacgaag ttatgggaaa
    10801 tgattccgtt acaaatacaa gttttaaaat atttttctag taaatataca aaatctaaag
    10861 tacgtaagca attgccaggt aagtttgctt acattatcga agaactttta gctgagattg
    10921 accgtaaccc agagaagaaa agctattttg atactattat tgaaaaatta tttgaattag
    10981 accaagtaga agatttaatt atagtattgt cccaaacaat tcaagtttta atcattgacc
    11041 atttacatgt tgtaggggat atttacgatc gtggtagata tcctgatcgt atcttgaata
    11101 ggttaatggc ttttccaaat ttggatatcc aatgggggaa ccatgatgtc acttggatgg
    11161 gagcggcatc tggttcctac ctttgtatgg taaatgttat ccgtattgct gcacgttata
    11221 ataatattac tttaattgaa gaccgatatg gtatcaattt gcgtcgcttg gttgattact
    11281 cacgccgtta ctatgaacct ctaccttctt tcgctcctat attagatgga gaggaaatga
    11341 ctcatccaga tgaactagac ttgttaaata tgatacaaca agcaacagca attttacagt
    11401 ttaaattgga agcccaattg attgacaggc gtccagaatt tcaaatgcat aatcggcaac
    11461 ttattaacca ggtaaattat aaagatttaa gtatatctat aaagggagtt gtgcatcaac
    11521 ttaaagattt taatagtaga tgcattgatt cgaaaaatcc cagtcgatta acaagtgagg
    11581 aagaagagct tttacaacaa ttgatgatag ccttccagac ttctgaaagt ttaaagaaac
    11641 atattgattt cctatttgaa aaaggaagta tgtatttaac ctataatgat aatcttttat
    11701 tccatggttg tataccaatg catagtaatg gtgattttaa aagctttaaa attgcaggaa
    11761 aaacttatgg tggtagggat ttactggatt tatttgaaag tcaaatacgt ctagcatatg
    11821 ctagaccaga gaaacatgat gatttggcga cagatatcat ttggtatctt tggtgtggtg
    11881 aaaattcttc tctttttggt aagaatgcca tgactacttt tgaacgctat tatgtttccg
    11941 acaaagtaac tcatcaagaa agaaaaaatc cctactttaa actgagagat aaagatgata
    12001 tttgcactac tcttttacaa gagtttgatt tgcctaagtt tgggcatatt gtcaatggtc
    12061 atacgccagt caaagaaaaa aatggtgagc aaccgataaa agcaaatggt aagatgttag
    12121 taattgatgg tggctttgcc aaaggttatc agaaaaatac aggtttagct ggctacactt
    12181 tgatttataa tagctatggg atacaattaa tttcacacct tccttttaca tccattgagg
    12241 aagttttaag tggaaccaac tatatcattg atacgaagcg attagtagaa gaagccaagg
    12301 ataggattct tgtaaaagat acaactattg gacaaaagtt aaccaaagag attaaagact
    12361 tagatcacct ttatcgtcat tttcaggaat atgatgacta gtgtttaagt aatttactaa
    12421 ttgatatgta ggatttaatt tttcagtttc ttatcttgtc tcttttatta aattgactta
    12481 aaataatatt atggacataa aggcagaaat tcaaaaatta gcaaaagaaa ttggtatctc
    12541 taaaattggt tttacgacgg cagataactt tgattatttg gaaaagtcat tgcgtgcttc
    12601 tgtggaagaa ggtcgcaatt caggatttga acataaagtc atagaggatc gtatctaccc
    12661 tgaaaggctt ttagaatcgg ctaaaacgat tatttcaatt ggcgttgctt atcctcacaa
    12721 acttcctcag caacctcaaa aaacacctta taaacgagga aaaattacac ctaattcgtg
    12781 ggggcttgat tatcattatg tggtcggcga aaagttagac agactatcaa aaggcattga
    12841 agagctctgt cgagattttc ctttgcaaca aaaagcaatg gtcgatactg gagctttagt
    12901 tgatactgca gttgcccaaa gagcaggtat tggttttata ggaaaaaatg gtctagttat
    12961 ttctaaagaa tatggctctt atatgttttt gggagaatta attaccaatt tagagatcga
    13021 gccagacaag cttgtcgatt atgattgtgg tgactgtcgt cgttgtctag atgcctgtcc
    13081 aacttcttgt ttaattggtg atggctcaat gaatgccaag cgttgtctat catttcaaac
    13141 ccaagataag ggaatgatgg acatagagtt tcgaaaaaag attaaaacag ttatctatgg
    13201 ttgtgatatt tgccaaattt gctgtcctta taacaaaggt ataaataatc cattggcgac
    13261 agagattgat cctgagttgg ctcaaccaga attaatacca tttttgagtt tatcaaatgg
    13321 acaatttaaa gaaaaatttg gtatgattgc tggaagttgg cgaggtaaaa atattttaca
    13381 gcgtaatgcc attattgcgc tagcaaatgc tcatgataaa accgcagtgg tcaaattaat
    13441 agagattatt gataaaaata ataaccctat tcatactgca acagctatat gggcattggg
    13501 agaaattgtc aaaaaaccaa acgatgagat actggagttt atgagtaatt taaccttaaa
    13561 agatgaagat agccgaaaag agttggaatt aatccgtcat aaatggcaat tttaagcctt
    13621 ttatggtatt tttaagaacc ttatgataaa ataaaacaga ttaacaaaga ttggagaaat
    13681 ctcatggaag tggctgaaat tcgccaaaaa atagtagaaa ataaagagaa gttgactagc
    13741 ttcaggaggt ctctttgact tagatcgttt ggaagaggag attgctcttc ttgaaaatca
    13801 aatgactgag ccagactttt ggaatgacaa tattgcagcg caaaagacat ctcaagaact
    13861 gaatgagtta aaaggtaagt atgatacttt ccataatatg caagaattat ctgacgaaac
    13921 agaactctta cttgagatgc ttgacgaaga tgattctttg aaagaagaat tagaagaaaa
    13981 tctaatgcag ctagataaga taatgggtgc ttatgaaatg acgcttctct tatctgagcc
    14041 ttatgaccac aataatgcta tcttagaaat ccatcctggt tcaggaggaa ctgaagcgca
    14101 agattgggga gatttactct tacgaatgta tacccgtttt ggcaatgcta atggtttcaa
    14161 agtagaagta ttggattatc aagcagggga tgaagctggt attaaatcgg ttaccctctc
    14221 ttttgaaggt cccaatgctt atggtttgtt gaaatcagaa atgggagttc atcgcctcgt
    14281 tcgtatatca ccttttgatt cggctaaacg tcgtcatacc tcatttgctt cagttgaagt
    14341 gatgccagag ttagatgata cgattgaagt agaagtacgt gatgatgaca ttaaaatgga
    14401 tactttccgt tccggaggag ctggtggtca gaacgtcaat aaagtatcaa caggtgttcg
    14461 tttgacgcat attccaactg gaattgtggt atcgtctacc gttgaccgta ctcaatatgg
    14521 gaaccgcgat cgcgctatga agatgttgca ggctaagcta tatcagttag aacaagaaaa
    14581 gaaagcacaa gaagtagatg ctctcaaggg tgataaaaaa gaaatcactt ggggaagtca
    14641 gattcgttct tatgttttca ccccttatac gatggtgaag gatcatcgta ctaactttga
    14701 gcttgctcag gtagataagg ttatggatgg agaaatcaat ggctttattg atgcctacct
    14761 taagtggcgc attgaagact aaaaataatg aaggagaaac atggcactaa tagaaatgtc
    14821 tggtgttact aaaaagtatc gccgttcaac aactgccttg agaaacttaa atttatcaat
    14881 tcagcaaggt gagtttgtgt atttagttgg tccgtcaggt gctggtaaat ctagcttgat
    14941 tcgcctccta taccgtgaag aaaaattaag ttcaggtaga ttaaaggtag gggaattcaa
    15001 tctcaataaa ctcaaacgtc gtcaaattcc tattttacgt cgtagtattg gagttgtctt
    15061 ccaagattat aaattactac caacaaaaac agtttatgag aatgttgcgt ttgcgatgca
    15121 agttattgga gctaagcgcc gtcatattaa aaaacgcgtg ccagaagttc tagaattagt
    15181 aggtttaaaa cacaagatgc gttcttttcc aacacagtta tctggtggag aacaacaacg
    15241 tgtggcaatt gcacgtgcga ttgttaataa tccaaaacta ttaattgcag atgaacctac
    15301 tggtaatttg gatccagaaa ttgcttggga aatcatgcat ctattggaac gtattaacct
    15361 tcaaggaacg actgttttga tggcaacaca caatagtcaa attgttaaca cacttcgtca
    15421 tcgtgtcatt gagattgaag caggtagcgt tatccgagat gaagagaagg gagaatatgg
    15481 ttatcatgat taatttcttt agacatttct gggaatcgct taaaaactta aaacgtaact
    15541 tttggatgac ttttgcttct gtaacatcag ttaccataac attacttctt gtaggcttgt
    15601 tttcatcagt tttactgaat gttgaaaagt taacaactga tgtttcaggt aattttacta
    15661 tcagtgcctt tttaaatgtt gactcaacgg atgctcaaaa acaagtcaaa gataaggacg
    15721 gaaagctaaa agataatcct gattatcata aagtttatga caagattaaa cgtatttctg
    15781 gtgtagagaa agtcacttat tcaagtaaag ctgaacaatt aaaagaagtt caaaaagagt
    15841 atggttcaga tgttattgat gatacctaca aagatgcttt gttagatgtt tacgtagttg
    15901 ggacaagttc tgcaaaagtt tctaagaatg tcagtgaagc aattggacgc attgaaggtg
    15961 ttgattatac taaagaacct attgattcaa caaaactatc taatctcaca gataacatcc
    16021 gtatatgggg atttggtggt gttgccttat taattgtcct tgctatcttt ttgatttcaa
    16081 atactattcg tatgtcgatt atgtcacgcc gtacagatat tgaaatcatg agattagttg
    16141 gcgctaaaaa ttcttatatt cgaggaccgt tcttttttga aggtgcttgg gtaggaattt
    16201 taggggctat cgttccatcg ctgattttct attttggtta tcaatttgtg tttaacaagt
    16261 ttaatccaaa atttgagaca agccatgttt cattgtatcc tatggatatt atggttccag
    16321 caattattgg tggtatggtg attattggta ttattattgg ctcacttggg tctgtcctat
    16381 caatgagacg ttaccttaag atttaataaa aagagatgaa atctatttca tctcttttta
    16441 ttaattgtag atatcatgct agtggataaa attcgaaata aacgtagcca attgtttttc
    16501 agatgatttg tgagaaaggg tagctttacc atcccctttt tgatgtccgt agttaccaaa
    16561 tcggctatgg tttcccccga caatagttcg aaaagtactt gaatttggga ggcgtttttt
    16621 agcttcctca tatttttccc agtttaaaat atgatcgtta cttgctgtaa tggagagtac
    16681 tcttaacttt ttatgagata ggtcggattt acttgaaggg taggaagcta ataatatgag
    16741 ccctctaaca ggggctactt tggcattttg gctagcaaca acgcctccta aagaatgtcc
    16801 agcaaggtaa acattagtta agtggttctg atcaataatt gttttagctt tataaggtga
    16861 taaaactggc aaatttaaag gtgtcttcaa aatataggta ttataccctt tatcagctaa
    16921 atctctagca agttgagaat aagcttcttc ttccacaaga gccccttgat agaaaataat
    16981 atttgctttt accttaccct tgcttctaaa aagaaggtag ttcttatggt ttgtagcggt
    17041 tttagcaatt ctcctagcta atggggaggc ttggtaagta ttgaagtaaa tggtcatccc
    17101 gatggccagt aaaatagcaa ggatacttgt tgttaaccaa agaaaaatag agtgacgttt
    17161 tatgttcatt ggaagaaagg attaaagttt ttttcgtggc caatagttgt attcatgcca
    17221 tggcctgggt gaacactata atgactaggt aaggtgaata gttcttggcg aataccagta
    17281 atgaggtcct caaagttact ggtaggtaag tcagtacgac cgatagtttc acgaaataga
    17341 gcatcgccag taacgactaa ttcatctgag tggaaaacaa atgagactcc tccccaagaa
    17401 tgaccaggtg ttggtaaaac tgtgaattca aaaccattta gttgataggg ttgctttaat
    17461 ttaaaaaagt tctctgccgg tcttgctata acattaatga tatcatcatg acggcctaac
    17521 ccagataaat tatcatctgg ggaagatagc caagcggctt ctttttctga aacataaaca
    17581 ggtggattat caaaagtatc tctgaccaaa tctaaactaa aaatatgatc atagtgagta
    17641 tgagttaaga ggatagccac taaaggtttt tcaaaggatt taattttagc aatgatttct
    17701 tggccattgc tccctggatc aatgaggata acagcttgat cattaacgag gtaataagta
    17761 ttttcaccag caatgtggtt tattgtttta tagattttca taatgatagt gtaccaaaaa
    17821 atcaagactt tgttaaaaaa agagtggcgg aaaataaatg gcaattagtt tcagaaaata
    17881 tgggatctca cagcaatttc ttgacaccgt tttcataaag gtgtatcata aaagtaatca
    17941 ataagccaag cctaaagctg aacttagttt aagaacactg actttttgag agcaacctaa
    18001 ttggttgact aaagaatatg aggtggaaga tatgtcaaaa gttgctattg ttactggtgc
    18061 tggtcaagga attggatttg ctattgcgaa acgtcttcat gcagacggct ttaaaatagg
    18121 cgttttagac tataatgaag agactgctca ggctgcggtt gataaactat cacctgagga
    18181 tgctgttgca gttgtagcag atgtttcaaa acgtgatcaa gtctttgatg ctttccaaaa
    18241 agttgtagat acttttggtg atttgaatgt tgttgttaac aatgcaggta ttgccccaac
    18301 aacccctctt gacactatta ctgaagagca gtttgaaaag gcttttgcaa tcaatgtcgg
    18361 aggaactatt tggggaagtc aagcagctca aaaacatttt agagaattag gtcatggcgg
    18421 aaaaattata aatgcaactt cacaagcagg atgtgaaggg aatcctaatt tgactgttta
    18481 tggtggtaca aaatttgcag ttcgtggtat tacacagact ttggcaaagg atttggcatc
    18541 agaaggaatc acagtcaatg cctacgcacc aggtattgtt aaaacaccta tgatgtttga
    18601 tattgcccat gaagttggta agaatgcagg caaagatgat gaatggggta tggagcagtt
    18661 tgcaaaagat atcactttga aacgcttgtc tgaaccagaa gatgttgcta atgcagtagg
    18721 cttcttagcg ggagatgatt caaattatat cactggacaa acaatagttg tcgatggtgg
    18781 tatggtattc cattaaaaat gattaatgct aacactgtga gtgaaactca cagtgttttt
    18841 tatatttctt aaaaaattat cttatcttgg ttattggagt gatggcatct ttgaggtaca
    18901 gtcatgtttt gttttataga tatagcgtgc tataatagac ttactatgac ccagaaaaaa
    18961 cttagaaaat atgcagtagt tgacttggag gctactggag caggtcccaa tgcttctatt
    19021 attcaggttg ggattgttat tatccaagga aataaaatta ttgatagtta tgaaacagat
    19081 gttaatcctc atgaatcttt ggatgagcat attgttcatt tgacaggtat tacggataag
    19141 cagttggtta aagctccgga ttttggtcaa gtggcgcatc acatttatca attgattgaa
    19201 gattgtattt ttgttgccca caatgtaaag ttcgatgcaa atttattggc tgagcaacta
    19261 tttttagaag gcttcgagtt acgtacacca aggatagata cagtagagtt atcacaagtg
    19321 ttttatccct gtttagaaaa atattcttta ggggcattag cagaaagctt aaatattgaa
    19381 ttaacagatg cgcacactgc tattgcagat gcaagagcta cagcacagtt attcattaag
    19441 ttaaaagcta aaattagtag tttacctaaa gaagtgttga agaccatttt aacatttgct
    19501 gataatcttt tgttcgaatc ctatttattg attgaagaag cttatcaaga agctgatttc
    19561 gttaatccta aagaatatta tttctggcaa ggattggtct taaaaaaaga aaaagctgtt
    19621 ggaaagccta agaaattatc tagtgatttt caagttaaca tggctttgtt aggtatggat
    19681 gctcgcccta agcaaattgt ttttgctgac ttggttaaag cgcattttaa tgatcaaaca
    19741 acaacgtttt tagaagctca atcaggactt ggtaaaacat atggatatct cttgccttta
    19801 ttggatcagt ctcaaaaaca acaaataata gtatctgttc caacaaagat tttgcaagat
    19861 caaataatgg ctaaagaaat aaagcacatt caagaacttt ttcatattcc ctgtcacagt
    19921 atcaaaggac cgagaaatta cttgaagtta gatgcgtttt ataagagcct ccaagtgcag
    19981 gatcgaaatc gattgataaa tcgttttaaa atgcaactgc tagtgtggtt gacggaaacg
    20041 actacaggtg atttggatga aattaaacaa aaacaacgat tagaatctta ctttgaccag
    20101 ctaaaacacg atggagaagt gactcaatca tcactttttt atgatctgga tttttggaaa
    20161 cgcagttatg ataaagttgc acaaagtcaa cttgttatta ttaatcatgc ttattttctt
    20221 gagcgtgttc aggatgacaa agattttgca aaggggaagg ttttagtttt tgatgaagct
    20281 cagaaacttg ttttgggact ggaaaatttt tctcgtgggc aacttgatat tagtcaccaa
    20341 ttgcaggtta ttcaaaaaat aattgatagt cctattccgt tattacaaaa gcgccttctg
    20401 gaaagtattt cttatgagtt aagtcatgca gcagaacttt tttatcgtca taattctttt
    20461 gaattttcag agacctggtt gaaacgtcta aaaaatagta taaacgcttt agaagtagta
    20521 ggattagatg atttacaaac ttttttcaca gctacttata ctaactattg gtttgaaact
    20581 gataaagtca atgagaagcg tttaaccatt ttacgtgggg cgagagaaga ttttcttaaa
    20641 ttttcagagt tcctccctcc cacaaaaaag acctatatga tatcagctac tttacaaatc
    20701 agtcccaaag tctacttgtc agatctttta ggaggatttt caagtataag cacagagaag
    20761 atagcgcatg agaaaaatgc caatcagaaa gtatggattg atacctctat gcctaatata
    20821 cttgatttat caccggaaca gtatgcttac gaaatagcca agcgtttaca agatataatg
    20881 acgttaaagc agcctacctt agtcttatta acttctaaac agacaatgtt tatggtatct
    20941 gattatttgg ataagtggga gataaaacat cttactcaag ataaaaatgg cttagcttat
    21001 aacgttaaaa aacgttttga tcgtggagaa agcaatcttc ttttaggtac aggatctttt
    21061 tgggaaggtg ttgactttgt acatcgtgat cgtcttattg aagttatcac gcgattgcct
    21121 tttgataatc ccaaagacta ttttatccag aaactatcgc agagtttaac aaaagagggg
    21181 aaaaacttct tttatgacta ttctttacca atgactgtct taaaattaaa acaggccttg
    21241 ggacgtacta caaggagaga agaacaaaag tcagcggtaa ttattttgga tagcagattg
    21301 gttattaaat cttatggaca aaccatcatg cattctcttg gtagagattt tgaaatttct
    21361 aaggaaaaaa ttaacaaggt cttgactgaa atggctaaat ttttgatata atggtaaaga
    21421 tcattgtcta aaaataagat tttgacagtg cgactcaaga ttaaataata gtagtacgtt
    21481 gataaaagga ggagtgatga cctatttgtc agaacgtgta ctcaatatgg aagagagtgt
    21541 tactttagct gcgggagcta aagcaagaga actcagagta caaggaagag atattcttag
    21601 tttaacttta ggggagcctg atttcgctac tcctaaaaac attcagcaag cggctattga
    21661 ggctataaca gatggacgtg caagttttta cacaccatca agtggtttgc cggagttgaa
    21721 gtcagcaatt aatgcgtatt ttgaacgttt ttatggctac tcccttaaac ctaatcaggt
    21781 ggttgtggga actggtgcta agtttatcct ttatactttc tttatgacag tactgaatcc
    21841 cggtgatgaa gtaattattc cgactcctta ttgggtatct tatgctgatc agattaagat
    21901 ggctgaaggt aagccagttt ttgtcacagc aaaagaagtt aatcacttca aagtaactgt
    21961 ggaacagtta gaagctgtta ggactgataa gacaaaagtc atacttctta atagtccatc
    22021 aaatcctaca ggtatgattt ataaagctga ggagttggag gctattggaa attgggcagt
    22081 cgaacatgat atcctaattt tagcagatga tatttacggt cgattggttt acaatggcaa
    22141 tatttttacc cctatttcaa gcctttcaga gtctattcga aaccaaacga tagtcataaa
    22201 tggagtttct aagacttatg cgatgacagg ttggcgtgtt ggttttgcag tgggtaatca
    22261 tgatattata gcggcaatgt ctaaagtagt cagtcaaaca acttctaacc ttacggctgt
    22321 ctcacagtat gctactattg aggctttaaa tggttctcaa gagtcatttg aaaaaatgag
    22381 gctagcattt gaagaacgcc ttaatatcat ttatccattg ttgtgtcagg tccctgggtt
    22441 tgaggttgtt aaaccacaag gtgcttttta tcttttccca aatgttacaa aagcaatgga
    22501 aatgaaagga tatacggatg ttacagcatt cacggatgcc attcttgaag aagttggact
    22561 tgctttagta acgggtgctg gttttggagc gccggaaaat gttcgtctga gttatgctac
    22621 agatcttgaa acccttaaag aagctgttcg tagactgcat gtctttatgg gaagtaatga
    22681 aattaattaa ataaaaatag agagaaactt atgtcaaaaa aacttatttc aattgttgat
    22741 gttaaagatt atgttggtca agaagttact attggtgctt gggttgctaa caaatcaggt
    22801 aaaggtaaga ttgcgtttgt acagttgcgc gatggttctg cctttttcca aggtgtcgct
    22861 tttaaaccaa acttcattga aaaatatggt gaagaatcag gtcttgaaaa atttgacgtt
    22921 attaaacgtc tcaaccaaga aacatctgtt tatgtaacag ggattgttaa agaggatgaa
    22981 cgttcaaaat ttggctatga gcttgatatt actgaccttg aagtcattgg agagtcacat
    23041 gaatatccta taactcctaa agagcatggg acagacttct tgatggacaa ccgtcatctt
    23101 tggttgcgtt ctcgtaaaca aatggctgtt atgcaaattc gtaacgctat tatttattca
    23161 acttatgaat tctttgacca aaatggtttt atcaaatttg atagcccaat tttatcagaa
    23221 aatgcagcag aagattcaac agagttattt gaaacagact attttgggaa accagcattt
    23281 cttagccaat ctggtcaatt ataccttgaa gctggagcaa tggctctagg ccgagttttc
    23341 gactttggtc cagtctttcg tgctgaaaaa tctaaaacac gtcgccattt aacagaattt
    23401 tggatgatgg atgctgagta ttctttcttg tcacatgagg aatcacttga cctccaagaa
    23461 gcttatgtta aagcattgat ccaaggcgtt ctagatcgtg cgcctcaagc acttgatatt
    23521 ttagaacgtg atgttgaggc tctcaaacgt tatatcgcag agccattcaa gcgtgtttct
    23581 tatgatgatg ctattacact tcttcaagag catgaagcag atgaagatac agattatgaa
    23641 catttagagc atggtgatga ctttggttca ccacatgaaa cttggatttc taattacttt
    23701 ggagtgccga catttgtagt gaattaccca gcaagcttta aagcatttta tatgaaacca
    23761 gttcctggaa atccagaacg cgttctttgt gctgatcttc tagctccaga aggttatgga
    23821 gagatcattg gcggctcaat gcgtgaagac gactacgatg ctcttgtggc taaaatggat
    23881 gaactaggta tggataaatc agaatacgac ttttaccttg accttcgtaa atacggctct
    23941 gttcctcatg gcggttttgg tataggtatt gaacgtatgg taacttttgt tgctggtacc
    24001 aaacatattc gcgaagctat tccattccca cgtatgttac atcgtatcaa accataataa
    24061 aaaagcgcta gctagtgcgc ttttttatta tggtctttag aatcgttggt taattaaatt
    24121 gggactatgt ttttttaaca atgtgattgt cgaaaagaca aggataagac tgattagtcg
    24181 atccatataa tctgttccaa cttgaacaag tatcaaacta cttgtcatgt ctaatccaat
    24241 tccatgtaag aattgtgcta taatacttga gccactagag gtaattcctt taaaaaggat
    24301 aactgttata actgaagcaa taatagttcc tggtaaagaa ataatcagtg atttccacaa
    24361 taggctagag ggtttacaat tccttttaat caagataccc gtaatgatag caaccacgat
    24421 ggcgactggt gaataataaa gtgaaaaaat atcagtagtc atccaattga tgagtgcact
    24481 gagaaaagca gtgctggcag cgtaccatgg tcccaggata actgctatta acaatgtacc
    24541 tatagtatct agatagatag gtaggcgtaa aaatagagct aaattagccc caacaaggtt
    24601 gatggcgata gcgattgaga taaatgaaat taaacgagca ggtgattttg tcatttgtta
    24661 agtcctaaca tttctaagtc ttcaatgatg actgaatttt gtgcatgtaa taggacggtc
    24721 ataaaatcat tccaaaataa attagtattg actgaggtta aaatcttact gttagcatct
    24781 tttttataaa aatgatactg atctacaatt gtttgtccca tggcgattcc gtgacaagca
    24841 acatcggtat agctatcaaa accagtagcg atattttcat taacaaagta agcaatagct
    24901 aaaggatcat ttatcacaca tccaataata tgctcatatt gccagtgaaa atcgaagtag
    24961 aactttgtga ttttttggat aaaacttgat acgtctggat taatacgctc catataagat
    25021 aaatgattgg gggttaatac aatatgacgt gtgatgtcta gtccaaccat ctctattttt
    25081 ttgtcaaggt tttcaaatac atattgtgca gcatgaggat cacaccaata gttgtattca
    25141 gcaactggag aacaattgcc atgtgattta aaacttcctc ccatgctaat aaatcgcttg
    25201 caatgtttgc ctaatttggg attagtttgt aaggctcgag cgatattggt cagaggtccg
    25261 agagctataa tagaagtatc atttttgtgt tcaaaatagt ttgctaaaaa acaatctgct
    25321 gattcttctt gaaagataat tggttgcgcc aatgtaaagt tattctctcc aaggccatcc
    25381 attccatgag tatcctgagc agatacaaat tcacgttgta aaggcaagtt atcaccaaca
    25441 taaactggaa tgtcgtgacg gtttagtaat tcaagcgtta caaaggtatt ctttagtcct
    25501 agctcaacag gcgaattgcc tgctgttata gtaatagcaa caacttctaa cttaggatgc
    25561 tgaatagcat acatcagtgc taaggtatca tcaataccgg gatcgcaatc gataataatt
    25621 ttttctttat tcatttgtct cctttctttt aatattagtg aatgttctat gcttttatga
    25681 aactattaac aatagtcatg gcaataaaaa agatcttggc agttaccaag atcacacgaa
    25741 aaaatgaatt tagttttcta aattcaatca tattttcagt cacttagttt tttattctgg
    25801 gaagttttcg aaccagtccg agaaatactc gattatgcac gtgttttatt ataatatgaa
    25861 agcactaact tgtaaagatt caaactgacc atttaacagt gtctagattg attgttttag
    25921 catttgaaat taattttgaa atcggacatc tgctgtgtgc tttgttaacg attagcttac
    25981 tatcagataa tgacagtttt tcaatactag caacagcgtt aacttggaaa taaaatccct
    26041 tacctacttg cttttccttc atgagctggc aagtaacatc aacgcgagat tttaggtctt
    26101 tgaatccttt ttgttctaaa atagctttta tagttgcatt taaacatgtt gaccatgcta
    26161 aagcaattaa ttcctcagga ttaaatcctg gtaaatgatt taaagggtga ttggtctcca
    26221 caatttttcc attggagaga taaacttttc catcaacgtc ttgagtgttt gatgaggtta
    26281 cttcataaag cataataaac tcctttgggt ctagtttacc aaatttggga gcattccgac
    26341 aagattttac tatttagaat gagtttcttt tcctctgaag taaaatccta attgctagcc
    26401 atttgtggta taatggcata aaacataaga gaaagaggta atcgtaatgt cagacgaaca
    26461 aatcaaactt gtgattgtaa caggtatgag tggggcagga aagacagtgg ctattcagtc
    26521 atttgaagat ttaggatatt ttacaataga taatatgccg cctactctag tgcctaagtt
    26581 tttagaatta gcagcacaat caggagatac aagcaagatt gctatggtag ttgacatgcg
    26641 tagtcgtctt ttctttagag aaattaactc tattttggat agccttgaaa ttaatgataa
    26701 tattaacttt aagattctat ttttagatgc tactgacacc gagctagtgt cacgatataa
    26761 agagacacgt cgtagccacc ctcttgctgc cgacggcaga gtgttagatg gtatttctct
    26821 agagagagaa ctattagcac cgcttaaaag tatgagtcag aacgttgtgg acactagtga
    26881 attaacacca agacaactac gtaaggtcat ttctgaagaa ttctcaaatc aagatagtca
    26941 aagttctttt cgtatcgaag ttatgagttt tggttttaaa tatggtatac ctttggatgc
    27001 ggatttagta ttcgatgtgc gttttttacc aaatccatac tataaaccag aattaagaga
    27061 taagacaggt ttagatactg aggtttatga ctatgtcatg agttttgatg aatcagatga
    27121 tttttatgac catcttcttg ccttaataaa gccaatctta ccaggctatc aaaatgaagg
    27181 gaaatctgtt ttgactgtgg caatcggatg tacaggtggg caacatcgta gcaccgcttt
    27241 tgctcataga ttatcagaag atttaaaagc agattggaca gttaatgaaa gccatcgtga
    27301 taaaaataag cgtaaggaga cggtaaatcg ttcatgagaa aacctaaaat aaccgttatc
    27361 ggcggaggaa cgggaattcc agttattttg aaaagtcttc gcttagaaga tgttgagatt
    27421 acagcggttg tcacggttgc agatgatggt ggttcgtcag gtgagttgcg ctcagttatg
    27481 caactaacac caccaggaga tctaagaaat gttttggttg ccttgagtga tatgccgaag
    27541 ttttacgaac aaattttcca atatcgtttt gcagaaggtg atggagattt tgcaggtcat
    27601 cctttaggaa atcttattat tgctggtgtt gctgaaatgc aaggttcaac ttataacgct
    27661 atgcagagtt taacacagtt ctttcataca acaggaaaga tatatccatc tagtgaacat
    27721 ccgctcactc tgcatgctgt ttttaaagat ggccatgaag tggttggaga gagtcaaata
    27781 gccgattata aaggcatgat tgaccatgtg tatgtcacta atacttataa tgaagagact
    27841 cctacggcca gccgcaaagt tgttgatgca attttagaaa gtgatatgat tgtgttgggt
    27901 ccagggtcac ttttcacctc tattttacct aacctagtta ttccagagat taaacaagct
    27961 cttttagaaa ctcgggcaga ggtagcttat gtatgcaata ttatgacaca acgtggtgaa
    28021 actgagcatt ttacagacgc agaccacgtt gaagtattga aacgtcattt aggacaagat
    28081 gctatcgata cagttttggt taatattgaa aaagtgccag agtcttatat ggaaaacaat
    28141 cattttgatg agtatttggt acaggttgaa catgattttt cagggcttag aaaacatgcc
    28201 agacgtgtca tctcgtctaa ctttttaaaa ttagaaaaag gtggagcctt ccatcatggt
    28261 gacttcgttg ttgaggaact aatgaattta gtaggaacac atttatgagt tttacagtaa
    28321 aagtcaaaga agagttgcta ggtcataaga gtgaaaataa gatggaactg tcagctatta
    28381 tcaaaatgtc aggtagttta ggattagcta accacggcct caacctttct attaccacag
    28441 aaaatgctaa aattgctcgt catatttata gcatgttaga agaacattat catttgcagc
    28501 cagaaatcaa ataccatcaa aaaacgaact tacgaaagaa tcgtgtgtat actgttttta
    28561 ttgaagaaaa agttgatgtt attcttgctg atttgaagtt ggctgatgct ttttttggta
    28621 ttgaaactgg tattgaacac tctatattgg acaatgatga aaatggaaga gcttatctca
    28681 ggggagcatt cttatcaacg ggtactgtta gagaacctga ttctggaaaa tatcaactag
    28741 aaattttttc tgtttatcta gaccatgcac aagacttagc taatcttatg aaaaaattta
    28801 tgttagatgc aaaggttatt gaacataaac atggagcagt aacttatttg caaaaagcag
    28861 aagatattat ggatttcttg atagtcattg atgccatgga agctagagat gcttttgaag
    28921 agattaaaat gatacgtgag acacgtaatg atattaaccg tgctaacaac gttgaaacag
    28981 ctaatattgc tagaacaatc acagctagta tgaagactat caataatatt atcaagatta
    29041 tggatactat tggttttgat gcactgccta gtgatttaag acaagtagct caggtgcgtg
    29101 tggctcatcc agattactct attcaacaaa tagcagacag tttagaaacg cctctgagta
    29161 agagtggcgt taaccatcgc ttacggaaaa taaataaaat tgcagatgaa ttatagaagg
    29221 agatattagc atggcttgta caacaatatt ggttggtaaa aaggcttctt atgatggttc
    29281 gactatgatc gctagaacgg aagactctgt taatggcgat ttcacaccca aaaaattaaa
    29341 ggtaatgaca tctaaagatc aaccgcgtca ttacaaatca gttttatcaa attttgaagt
    29401 agatttacca gataacccac ttccttatac ttcagtaccg gacgcattgg gaaaagatgg
    29461 tatatggggt gaagccggta ttaacagtaa aaatgtagcg atgagtgcta cagaaactat
    29521 tacaacgaat tcccgcgttt tgggtgcaga tcctttggtt tcagatggta taggggaaga
    29581 ggatatactc actttagtgc ttccctatat tcagtcagcg cgagaaggtg tggagcgttt
    29641 aggtgctatt ttggaaaaat atggaaccta tgaatcaaat ggtattgctt tttcagatac
    29701 cgaagaaata tggtggttag aaacaattgg tgggcatcat tggattgctc gtcgcgtacc
    29761 tgatgatgtt tatgttacta atcctaacca actaggaatt gatcattttg aatttaataa
    29821 ctgtgatgac tacatgtgct ctagtgattt gaaagagttt atcgaacaat accatttaga
    29881 tttgacctat tctaatgagc atttcaatcc tcgatatgct tttggtagcc aacgtgataa
    29941 agatcgtcat tacaacacac caagaagttg ggcaatgcag cgttttttaa atcctgaaat
    30001 tgaacaggat ccacgtagct tgtttattcc ctggtgtcaa aaaccttacc gaaaaattac
    30061 tgttgaggat attaaatatg tgttgagtga tcattatcaa gacagtgtgt atgacccata
    30121 tggaccagaa ggggatgcgg taagtaggag agcttttcgt tcagttggta tcaaccgaac
    30181 tagtcaaacg tctattctac aattacgacc aaataaatca cttgaaacga caggtgttca
    30241 atggttatct tatggctcta tgccatttgc aaccatggtg ccgttgttta cacaagttga
    30301 gactgtacca gactattttt cgaatacaac caaggatgct tcaacagata atttttattg
    30361 gaccaatcgt ttaattgcag ctctagcaga tccacacttt tatcaacatg aagctgatat
    30421 tgaaagctat atcgagagaa cgatggccca aggacatgca catattaacg gtattgatag
    30481 agaagttgct gagaataaag agattgattt tcaacagaaa aatcaagaga tgagtgacta
    30541 tattcaaaaa gaaagccaag aattgttaaa tcgtattcta tttgatgcaa gtaatttaat
    30601 gacaaatcgc ttttcaatgg gagattaaaa aactcggaat attttccggg ttttttaaga
    30661 taaaaacgct tgcataagta tataaaactt gcaaaaaaaa aatatcgtgc tatactttac
    30721 tagtaaaata ttaaccggta aataacaggt taaataaaaa ggagaaaaaa tgagaaagaa
    30781 atttctttta ttgatgagct ttgtagctat gtttgcagct tggcaacttg ttcaagttaa
    30841 acaagtttgg gctgatagta aacttaaagt ggtaacaact ttttacccag tttatgagtt
    30901 tacaaaaaat gtcgttggtg ataaagctga tgtatctatg ttaattaaag caggtacaga
    30961 gccgcatgat tttgaaccat caactaaaaa catcgctgcc atccaagatt caaatgcttt
    31021 tgtttacatg gatgataaca tggaaacttg ggctccaaaa gtagctaagt cagttaaatc
    31081 caaaaaagta acaactatta aaggtactgg cgatatgtta cttactaaag gcgtcgaaga
    31141 agaaggtgaa gatcatgaac atgaaggaca tggtcatgaa gggcatcatc atgaacttga
    31201 cccacacgta tggttgtctc cagaacgtgc gatttctgtt gtagaaaaca tccgtaataa
    31261 atttgtcaaa gcttatccaa aagatgcagc ttcatttaac aaaaatgcag atgcttacat
    31321 tgcaaaatta aaagatcttg acaaagaata caaaaatggt ttgtcaaatg ctaaacaaaa
    31381 gagttttgtg actcaacacg cagcgtttgg ttacatggcg cttgattacg gtttaaatca
    31441 agttccaatt gctggtctta ctccagatgc agaaccttca tcaaaacgtt taggcgaatt
    31501 agctaaatac atcaagaaat ataacatcaa ctacatttat tttgaagaaa atgcttcaaa
    31561 taaagttgct aaaactttag cagatgaagt tggcgtgaaa acagctgtgc ttagtccact
    31621 tgaaggactt tctaaaaaag aaatggcagc tggcgaagat tacttctcag ttatgagacg
    31681 taatttgaaa gttcttaaaa agacaacaga tgttgcaggt aaagaagtag ctcctgaaga
    31741 agataaaact aaaacagttg aaacaggtta ctttaaaact aaagatgtta aagaccgtaa
    31801 attgacagat tactctggta attggcaatc agtatatcct cttcttcaag atggaacact
    31861 tgatccagtt tgggattaca aagctaaatc taaaaaagat atgactgctg cagagtacaa
    31921 aaaatattat acagcaggtt acaagactga cgtagaatca atcaagattg atggtaaaaa
    31981 acatcaaatg acctttgtac gtaatggtaa atcacaaaca tttacataca aatatgcagg
    32041 ttacaaaatc ttaacttata aaaaaggtaa tcgtggagta cgttatctct ttgaagctaa
    32101 ggaaaaagat gctggtcaat tcaaatatat ccaatttagt gaccatggta ttaaaccgaa
    32161 taaagctgaa cacttccata tcttctgggg ttcagaaagc caagaaaaat tatttgagga
    32221 aatggaaaac tggccaacat acttcccagc taaaatgtct ggacgtgaag ttgcccaaga
    32281 ccttatgtct cattaatatt tgaaagatga tatcggatac gatatcatct ttttttcttc
    32341 gtacgtgata ggctcaaaca tagaaaaaag cacttctaat agaagtgctt ttaaaattaa
    32401 tgctatgctt attgagcagc gtttgcgtct ttgagaccat attttttgtt gaaacggtcc
    32461 acacgtccat ctgcttgagt gaatttttga cgtcctgtat agaatggatg tgaatctgat
    32521 gagatttcca cacggataag tgggtaagtt tcaccttcga attctactgt ttctttagtt
    32581 gatttagttg aaccactaag gaatttgtaa cctgtagttg tatctaagaa aacaactgga
    32641 cgataatctg gatggatgtc ttttttcatc tttaaaaatt tcctttctgc cataactcgt
    32701 ctgagtcata gttattaact actctattct atcaaataag aatagccttg acaagtgttt
    32761 tgataaaaaa agtaaaatta taaagaaagt acttcctgga tttcttggta aatagcttga
    32821 ttctcttcca aactataaga attagctcca ctggctaaag gatgtccgcc accattatga
    32881 cgtttagcta tttcattaat gatgtgtgac ttactacgta ggcgcacacg ataatgccca
    32941 tcgctttgtt caacgaaaat tgcccaactt tctacaatat caatttttcc aggtacacca
    33001 acgattgcag cagtctctgc atctgttaca tcaaagcgtt ttaagtcttc ttgtgttaaa
    33061 gtaacgcatg cagcgccgtt cttgtcaatt ttaagttgtt caaaaatgaa gccttgtaat
    33121 tttgcaattt tgaaaggaaa ggaatccatt tgacgtgcca ttgcagaaaa atcaaagtca
    33181 aattcacgaa gcttgctagc aatcttcagt gttttacttg tggtagcagg atataaaaag
    33241 cgtcctgtat ctcctacaat accattatat agaactcttg ctgcacttgt tgataacaac
    33301 aaatcacaag aaagagcaaa gtctgtcaca atttcacttg cactagaggc gttagtatct
    33361 acataagaga gatctccata gatttcatcg tttggatgat ggtcaatttt aattaaaaag
    33421 tcaccttttt tataacgttc atcatcaatg cgtggggtat ttgctgtatc tgtaacaata
    33481 acaagagcgc cttgatagtc ttggtcggtc acttgatcca ttttagcaat ccaagtgagt
    33541 gtgggttcgt caaaaccagt agcaagtact tttttctttg ggaaattatg cctgatgata
    33601 tcccttaagc cgatttggct accaagagca tcagggtctg gtctcatatg tcgatggata
    33661 atgatggtgt cgtactcttt gattttatca agaatttgtt gaaaaataat cattttttac
    33721 ctacctaact gttttgtctt ttagtgaaga acaaggctat atcgttgact atattatagc
    33781 ttaaatcttt tattgaggaa agaaattaag atagcggt
//
LOCUS       DBJYSH010000006        32823 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.006, whole genome shotgun sequence.
ACCESSION   DBJYSH010000006 DBJYSH010000000
VERSION     DBJYSH010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 32823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32823
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.006"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            180..380
                     /locus_tag="ACUBV3_000244"
     CDS             180..380
                     /locus_tag="ACUBV3_000244"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HHU3846781.1"
                     /translation="MNLAILALGFAVMGVSIGEGILVANIAKSAARQPEMFSKLQTLM
                     FTGVAFIEGTFFVLFAFTFLVR"
     gene            412..1128
                     /gene="atpB"
                     /locus_tag="ACUBV3_000245"
     CDS             412..1128
                     /gene="atpB"
                     /locus_tag="ACUBV3_000245"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000446422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HHU3846782.1"
                     /translation="MESTSNPTVSFLGIDFDLTILAMSLLTITIIFILVFWASRKMTI
                     KPKGKQNVLEYVYELVNNTISQNLGHYTKNYSLLMFILFSFVFIANNLGLMTSLKTHE
                     HNFWTSPTANFGVDITLSLLVAFICHIEGIRKKGIGGYLKGFLSPTPAMLPMNLLEEV
                     TNVASLALRLFGNIFSGEVVTGLLLQLAVLSPFTGPLAFALNIVWTAFSMFIGFIQAY
                     VFIILSSSYIGHKVHGDEEE"
     gene            1146..1643
                     /gene="atpF"
                     /locus_tag="ACUBV3_000246"
     CDS             1146..1643
                     /gene="atpF"
                     /locus_tag="ACUBV3_000246"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000025481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HHU3846783.1"
                     /translation="MSILINSTTIGDIIIVSGSVLLLFILIKTFAWKQITGIFEAREQ
                     KIANDIDTAEQARQQAEAFATKREEELSNAKTEANQIIDNAKETGLAKGDQIISEAKT
                     EADRLKEKAHQDIAQNKAEALADVKGEVADLTVLLAEKIMVSNLDKEAQSNLIDSYIK
                     KLGDA"
     gene            1643..2179
                     /locus_tag="ACUBV3_000247"
     CDS             1643..2179
                     /locus_tag="ACUBV3_000247"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HHU3846784.1"
                     /translation="MNKKTQALIEQYSKSLVEVAIEHKIVEEIQQEVAALIDIFETSE
                     LEGVLSSLAVSHDEKQHFVKTLQTSCSTYLVNFLEVIVQNEREALLYPILKSVDQELI
                     KVNGQYPIQITTAVALSPEQKERLFDIAKTKLALPNGQLVEHIDPSIVGGFVVNANNK
                     VIDASVRNQLHQFKMKLK"
     gene            2195..3700
                     /gene="atpA"
                     /locus_tag="ACUBV3_000248"
     CDS             2195..3700
                     /gene="atpA"
                     /locus_tag="ACUBV3_000248"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HHU3846785.1"
                     /translation="MAINAQEISALIKKQIEDFQPNFDVTETGIVTYIGDGIARARGL
                     DNAMSGELLEFSNGAYGMAQNLESNDVGIIILGDFSEIREGDVVKRTGKIMEVPVGEA
                     MIGRVVNPLGQPVDGLGEIETTATRPVETPAPGVMQRKSVFEPLQTGLKAIDALVPIG
                     RGQRELIIGDRQTGKTSVAIDAILNQKGQDMICIYVAIGQKESTVRTQVETLRKYGAL
                     DYTIVVTASASQPSPLLFIAPYAGVAMAEEFMYNGKHVLIVYDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERSAKVSDALGGGSITALPFIETQAGDISAYIATNV
                     ISITDGQIFLQENLFNSGIRPAIDAGSSVSRVGGAAQIKAMKRVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEVLKQPLHKPLPVEKQVVILYALTHGFLDDVPV
                     NDILAFEEALYDYFDAHYDNLFETIRTTKDLPEEAELDAAIQAFKDQSQFK"
     gene            3716..4597
                     /locus_tag="ACUBV3_000249"
     CDS             3716..4597
                     /locus_tag="ACUBV3_000249"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HHU3846786.1"
                     /translation="MAGSLSEIKDKILSTEKTSKITSAMQMVSSAKLVKSEQAARDFQ
                     VYASKIRQITTNLLKSDLVSGSDNPMLASRPVKKTGYIVITSDKGLVGGYNSKILKAM
                     MDTITDYHTENDDYAIISIGSVGSDFFKARGMNVSFELRGLEDQPSFDQVGKIIAQAV
                     EMYKNELFDELYVCYNHHVNSLTSQVRMQQMLPIKELDAEEASEDRVITGFELEPNRE
                     VILEQLLPQYTESLIYGAIIDAKTAEHAAGMTAMQTATDNAKNVINDLTIQYNRARQA
                     AITQEITEIVAGANALE"
     gene            4671..6077
                     /gene="atpD"
                     /locus_tag="ACUBV3_000250"
     CDS             4671..6077
                     /gene="atpD"
                     /locus_tag="ACUBV3_000250"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HHU3846787.1"
                     /translation="MSSGKIAQVVGPVVDVVFASGDKLPEINNALIVYKNGDKSQKVV
                     LEVALELGDGLVRTIAMESTDGLTRGLEVLDTGRAISVPVGKDTLGRVFNVLGDAIDL
                     EEPFAEDAERQPIHKKAPSFDELSTSSEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYWEMKESGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSVTSIQAIYVPADDYTDPAPATAFAHLDSTTNLERKLTQ
                     MGIYPAVDPLASSSRALTPEIVGDEHYEVATEVQRVLQRYRELQDIIAILGMDELSDE
                     EKTLVGRARRIQFFLSQNFNVAETFTGQPGSYVPVEETVRGFKEILDGKHDQIPEDAF
                     RMVGGIEDVIAKAEKMNY"
     gene            6090..6503
                     /locus_tag="ACUBV3_000251"
     CDS             6090..6503
                     /locus_tag="ACUBV3_000251"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000068055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HHU3846788.1"
                     /translation="MAQLTVQVVTSDGIRYDHHASLITVRTPDGEMGILPGHINLIAP
                     LIVHQMKINRSHQEGVDWVAVNGGIIEVNEDQVTIVADSAERARDIDLNRAERAKERA
                     ERALEKAQTTQNIDEMRRAEVALRRAINRISVGKK"
     gene            6568..6798
                     /locus_tag="ACUBV3_000252"
     CDS             6568..6798
                     /locus_tag="ACUBV3_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 family protein"
                     /protein_id="HHU3846789.1"
                     /translation="MILNIVTIVSNFIFIALFYQLFVDLFDWSKMIKMSPQNISKLKV
                     FILLISIVGGYVVSHFLLEVIQLCQSIFWALQ"
     gene            6861..8132
                     /gene="murA"
                     /locus_tag="ACUBV3_000253"
     CDS             6861..8132
                     /gene="murA"
                     /locus_tag="ACUBV3_000253"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HHU3846790.1"
                     /translation="MDKIIVEGGQTRLQGQVVIEGAKNAVLPLLAATILPSQGKTLLT
                     NVPILSDVFTMNNVVRGLDIQVDFNCDKKEILVDASGDILDVAPYEFVSQMRASIVVL
                     GPILARNGHAKVSMPGGCTIGSRPIDLHLKGLEAMGATITQNGGDITAQAEKLKGANI
                     YMDFPSVGATQNLMMAATLASGTTTIENAAREPEIVDLAQLLNKMGAKVKGAGTETLT
                     IIGVDALHGTEHDVVQDRIEAGTFMVAAAMTSGNVLVKDAIWEHNRPLISKLMEMGVE
                     VSEEEDGIRVKADTKKLKPVTVKTLPHPGFPTDMQAQFTALMAVVNGESTMIETVFEN
                     RFQHLEEMRRMGLQTEILRDTAMIHGGRALQGAPVMSTDLRASAALILAGMVAQGQTV
                     VGQLTHLDRGYYQFHEKLAALGANIKRVSEA"
     gene            8134..8325
                     /locus_tag="ACUBV3_000254"
     CDS             8134..8325
                     /locus_tag="ACUBV3_000254"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HHU3846791.1"
                     /translation="MASSGWKYVLKQIGLIVLVILLALLFLAVGLMLGYSVFGDGEHA
                     YSILSLDKWQNIIGKFLGK"
     gene            8400..9257
                     /locus_tag="ACUBV3_000255"
     CDS             8400..9257
                     /locus_tag="ACUBV3_000255"
                     /EC_number="3.1.30.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HHU3846792.1"
                     /translation="MTKKQKQITASIVTLLFSLLAIGLGESKSLPNDHILKQVTDLII
                     SNESGKNTSLKNISGTPSRELSESVLTSNVKKQLGTNIAWNQSGAFIINQNKTDLNAK
                     VSSAPYAINEIKKVNNQIVPTKANALLTKATRQYRNREETGNGRTYWKPAGWHQINGL
                     KGSYNHAVDRGHLIGYALVGSLRGFDASTSNPKNIATQAAWANQANSNQSTGQNYYET
                     LVRKALDRHKTVRYRVTLIYDRDNLLSSGSHIEAKSSDGSLEFNVFIPNVQSGLLFDY
                     ATGKVKQTK"
     gene            9548..10588
                     /gene="pheS"
                     /locus_tag="ACUBV3_000256"
     CDS             9548..10588
                     /gene="pheS"
                     /locus_tag="ACUBV3_000256"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000365656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HHU3846793.1"
                     /translation="MDLQKQLEELKISTQEKLKEMTGNHTKELQDLRVQVLGKKGSLT
                     ELLKGLKDLSNDLRPVVGKQVNEVRDILTKAFEEQAKVVEAAKIQAQLESESVDVTLP
                     GRQMTLGHRHVLTQTSEEIEDIFLGMGFQVVDGFEVEKDYYNFERMNLPKDHPARDMQ
                     DTFYITEEILLRTHTSPVQARTMDQHDFSKGPLKMISPGRVFRRDTDDATHSHQFHQI
                     EGLVVGENISMGDLKGTLQLISQKMFGAERKIRLRPSYFPFTEPSVEVDVSCFKCGGK
                     GCNVCKQTGWIEILGAGMVHPSVLEMSGIDSEKYSGFAFGLGQERIAMLRYGINDIRG
                     FYQGDVRFTDQF"
     gene            10671..11191
                     /locus_tag="ACUBV3_000257"
                     /pseudo
     CDS             10671..11191
                     /locus_tag="ACUBV3_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078399.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            11245..13650
                     /gene="pheT"
                     /locus_tag="ACUBV3_000258"
     CDS             11245..13650
                     /gene="pheT"
                     /locus_tag="ACUBV3_000258"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HHU3846794.1"
                     /translation="MLVSYKWLKELVDVDVTTAELAEKMSTTGIEVEGVETPAEGLSK
                     LVVGHIVSCEDVPDTHLHLCQVDTGDDELRQVVCGAPNVKTGINVIVAVPGARIADNY
                     KIKKGKIRGMESLGMICSLQELGLSESIIPKEFSDGIQILPEGAIPGDSIFSYLDLDD
                     EIIELSITPNRADALSMRGVAHEVAAIYGKKVHFEEKNLIEEAERAADKISVVIESDK
                     VLSYSARIVKNVTVAPSPQWLQNKLMNAGIRPINNVVDVTNYVLLTYGQPMHAFDFDK
                     FDGTTIVARNAENGEKLITLDGEERDLIADDLVIAVNDQPVALAGVMGGQSTEIDSSS
                     KTVVLEAAVFNGTSIRKTSGRLNLRSESSSRFEKGINYDTVSEAMDFAAAMLQELAGS
                     QVLSGQVTEGVLPTEPVEVSTTLGYVNTRLGTELTYTDIEEVFEKLGFAISGSEVKFT
                     VLVPRRRWDIAIQADLVEEIARIYGYEKLPTTLPEAGATAGELTSMQRLRRRVRTVAE
                     GVGLSEIITYALTTPEKAVQFSTQATNITELMWPMTVDRSALRQNVVSGMLDTIAYNV
                     ARKNSNLAVYEIGKVFEQTGNPKEDLPTEVETFTFALTGLVEEKDFQTKAKPVDFFYA
                     KGIVEALFIKLKLDVTFVAQKGLASMHPGRTATILLDGKEIGFVGQVHPQTAKQYDIP
                     ETYVAEINLSTIESQMNQALIFEDITKYPSVSRDIALLLAESVSHHDIVLAIESSGVK
                     RLTAIKLFDVYAGNNIAEGYKSMAYSLTFQNPNDNLTDEEVAKYMEKITKSLVEKVNA
                     EIR"
     gene            complement(13719..14621)
                     /gene="ypfJ"
                     /locus_tag="ACUBV3_000259"
     CDS             complement(13719..14621)
                     /gene="ypfJ"
                     /locus_tag="ACUBV3_000259"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KPN_02809 family neutral zinc metallopeptidase"
                     /protein_id="HHU3846795.1"
                     /translation="MKIDDLRKSDNVEDRRSSSGGSFSSGGSGLPILQLLLMRGSWKT
                     KLVVLIILLLLGGGGLTSIFNDSSSPSSYQSQNVSRSVDNSATREQIDFVNKVLGSTE
                     DFWSQEFQTQGFGNYKEPKLVLYTNSIQTGCGIGESASEPFYCSADKKIYLDISFYNE
                     LSHKYGATGDFAMAYVIAHEVGHHIQTELGIMDKYNRMRHGLTKKEANALNVRLELQA
                     DYYAGVWAHYIRGKNLLEQGDFEEAMNAAHAVGDDTLQKETYGKLVPDSFTHGTAQQR
                     QRWFNKGFQYGDIQHGDTFSAEHL"
     gene            14799..18032
                     /gene="rexB"
                     /locus_tag="ACUBV3_000260"
     CDS             14799..18032
                     /gene="rexB"
                     /locus_tag="ACUBV3_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent nuclease subunit B"
                     /protein_id="HHU3846796.1"
                     /translation="MKLLYTDINHDMTEILVNQAAHAAEAGWRIFYIAPNSLSFEKER
                     AVLETLPQEASFAITITRFAQLARYFTLNQPNQKESLNDIGLAMIFYRALASFEDGQL
                     KVFGRLKQDASFISQLVDLYKELQTANLSILDLKYLHSPEKFEDLLAIFLVVSDLLRE
                     GEYDNQSKIAFFTEQVRSGQLDVDLKNTILIVDGFTRFSAEEEALIKSLSSRCQEIII
                     GAYASQKAYKTNFTNGNIYSAGVDFLRYLATTFQTKPEFILSKWESKSGFEMISKNIE
                     GKHDFTNSSHILDDTAKDCITIWECINQKDEVEHVARAIRQKLYQGYRYKDILVLLGD
                     VDSYKLQLSKIFEQYDIPYYFGKAETMAAHPLVHFMDSLSRIKRYRFRAEDVLNLFKT
                     GIYGEIGQDDLDYFEAYISYADIKGPKKFFTDFVVGAKKFDLGRLNTIRQSLLTPLES
                     FVKTKKQDGIKTLNQFMFFLTQVGLSDNLSRLVGQMSENEQEKHQEVWKTFTDILEQF
                     QTIFGQEKLNLDEFLSLLNSGMMQAEYRMVPATVDVVTVKSYDLVEPHSNQFVYALGM
                     TQSHFPKIAQNKSLISDIERQLINDANDTDGHFDIMTRENLKKNHFAALSLFNAAKQA
                     LVLTIPQLLNESEDQMSPYLVELRDIGVPFNHKGRQSLKEEADNIGNYKALLSRVVDL
                     YRSAIDKEMTKEEQTFWSVAVRYLRRQLTSKGIEIPIITDSLDTVTVSSDVMTRRFPE
                     DHPLKLSSSALTTFYNNQYKYFLQYVLGLEEQDSIHPDMRHHGTYLHRVFEILMKNQG
                     IESFEEKLNSAINKTNQEDVFKSLYSEDAESRYSLEILEDIARATATILRQDSQMTVE
                     SEEERFELMIDNTIKINGIIDRIDRLSDGSLGVVDYKSSAQKFDIQKFYNGLSPQLVT
                     YIDAISRDKEVEQKPPIFGAMYLHMQEPRQDLSKIKNLDDLVTKNHQALTYKGLFSEA
                     EKEFLANGKYHLKDSLYSEAEIAILQAHNQSLYKKASETIKSGKFLINPYTEDAKTVD
                     GDQFKSITGFEADRHMARARALYKLPAKEKRQGFLTLMQQEEENDDL"
     gene            18022..21645
                     /gene="addA"
                     /locus_tag="ACUBV3_000261"
     CDS             18022..21645
                     /gene="addA"
                     /locus_tag="ACUBV3_000261"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HHU3846797.1"
                     /translation="MTFKPFLNPEDIAVIQTEEKNSDKKQKRTPEQIEAIYTFGNNVL
                     VSASAGSGKTFVMVERILDKLLRGVPIESLFISTFTVKAAGELKERLEKKINESLKSA
                     ESDDLKQFLTQQLVGIQTADIGTMDAFTQKIVNQYGYTLGISPIFRILQDKNEQDVIK
                     NEVYADLFSDYMTGKNAASFIKLVKNFSGNRKDSKAFREMVYKVYAFSQSIDNPKRWM
                     QTVFLKGAQTYTDFEAIPDQEVSSLLNVMQTTANQLRDLTDQEDYKQLTAKGVPTANY
                     KKHLKIIENLVHWSQDFNLLYGKKGLANLARDITNVIPSGNDVTVAGVKYPIFKQLHN
                     RIVGLKHLEVIFKYQGESLFLLELLQSFVLDFSEQYLQEKIQENAFEFSDIAHFAIQI
                     LEENHDIRQIYQDKYHEVMVDEYQDNNHTQERILELLSNGHNRFMVGDIKQSIYRFRQ
                     ADPQIFNDKYKAYQDNPSQGKLIILKENFRSQSEVLDSTNSVFTHLMDEEVGDILYDE
                     SHQLKAGSPRQQERHPNNKTQVLLLDTDEDDIDDSDSQQYDISPAEAKLVAKEIIRLH
                     KEENVPFQDITLLVSSRTRNDGILQTFDRYGIPLVTDGGEQNYLKSVEVMVMLDTLRS
                     IDNPLNDYALVALLRSPMFGFNEDDLTRIAIHDVKMAFYHKVKLSYHKEGHHSDLITP
                     ELSSKIDYFMKTFQTWRDFAKWHSLYDLIWKIYNDRFYYDYVGALPKAEQRQANLYAL
                     ALRANQFEKTGFKGLSRFIRMIDKVLENENDLADVEVALPQNAVNLMTIHKSKGLEFK
                     YVFILNIDKKFSMVDITSPLILSRNQGIGIKYVADMRHELEEEILPAVKVSMETLPYQ
                     LNKRELRLATLSEQMRLLYVAMTRAEKKLYLVGKASQTKWADHYDLVSENNYLPLASR
                     ETFVTFQDWLLAVHETYKKQELFYDINFVSLEELTDHHIGMVNPSLPFNPDNKAENRQ
                     SEDIVHAISVLESVEQINQTYKAAIELPTVRTPSQVKKFYEPILDIEGVDVMETITKT
                     SVDFKLPDFSTSKKQDPAALGSAVHELMQRIEMSSHVKMEDIQKALTEVNAETSIKAA
                     IQIEKINYFFQENSLGKYIQEEVEHLHREAPFAMLKEDPESGEKFVVRGIIDGYLLLE
                     NRIILFDYKTDKFVNPLELKERYQGQMALYAEALKKSYEIERIDKYLILLGGKQLEVV
                     KMD"
     gene            21658..22575
                     /locus_tag="ACUBV3_000262"
     CDS             21658..22575
                     /locus_tag="ACUBV3_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HHU3846798.1"
                     /translation="MIVEQKFGNGFTWINIEAEQLRTETSEIQAKYLDSEIITYALDD
                     YERAFMECSHIKGKEVLTIIFNTIDLKQKESYYETVPMTFCLSHDRLITVTRSRNSYM
                     LELLQKYLDRNPDVSPKKFLFAALTLITKQYFNVVSKIDREKDILNRQLREQTTNKRL
                     LAMSDLETGSVYLLTAANQNALVLEQLDVHPSQRFNSEVEKEQLSDALIEAHQLVSMT
                     QLNSQVLSQLSSTFNNVLNNNLNENLTGLNIISINLAIIAAITGFFGMNIPLPLTESR
                     SSWLIVIATSVLLWVIIAQILKRVFNLRK"
     gene            complement(22607..23983)
                     /gene="mnmE"
                     /locus_tag="ACUBV3_000263"
     CDS             complement(22607..23983)
                     /gene="mnmE"
                     /locus_tag="ACUBV3_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HHU3846799.1"
                     /translation="MSITKEFDTIAAISTPLGEGAIGIVRISGTDALKIASKIYRGKD
                     LSAIQSHTLNYGHIVDPDKNEILDEVMLGVMLAPKTFTREDVIEINTHGGIAVTNEIL
                     QLILRHGARMAEPGEFTKRAFLNGRVDLTQAEAVMDLIRAKTDKAMDIAVKQLDGSLK
                     TLINNTRQEILNTLAQVEVNIDYPEYDDVEEMTTTLMREKTQEFQALMENLLRTARRG
                     KILREGLSTAIIGRPNVGKSSLLNNLLREEKAIVTDIEGTTRDVIEEYVNIKGVPLKL
                     VDTAGIRDTDDIVEKIGVERSKKALEEADLVLLVLNSSEPLTLQDRSLLELSKESNRI
                     VLLNKTDLPQKIEVNELPENVIPISVLENENIDKIEERINDIFFDNAGMVEHDATYLS
                     NARHISLIEKAVDSLKAVNEGLELGMPVDLLQVDMTRTWEILGEITGDAAPDELITQL
                     FSQFCLGK"
     gene            24111..26021
                     /locus_tag="ACUBV3_000264"
     CDS             24111..26021
                     /locus_tag="ACUBV3_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HHU3846800.1"
                     /translation="MIILQGNKIERSFSGDVLFDNINIQVDQRDRIALVGRNGAGKST
                     LLKILVGEEAPTKGEINKKRDLSLSYLAQDSRFQSENTIFQEMLQVFDSLREVEKRLR
                     ELELQMGQVSGSDLEQLMKTYDILSEEFREKGGFTYESDIKAILNGFKFNSDMWEMPI
                     SELSGGQNTRLALAKMLLEKPELLVLDEPTNHLDIDTIAWLENYLVNYQGALIIVSHD
                     RYFLDKVATVTYDLTTHSLDRYVGNYSKFMDLKAEKIATEEKNFEKQQKEIAKLEDFV
                     QRNIVRASTTKRAQARRKQLEKMERLDKPNVEQKSANMTFHAGKVSGNVVLTLENAAI
                     GYEGVSLSEPIDLDVKKFDAIAIVGPNGIGKSTLIKSLVGQIPFIKGEAKLGANVETG
                     YYDQSQSNLTKTNTVLDELWNAFSTTPEVEIRNRLGAFLFSGDDVKKSVSMLSGGERA
                     RLLLAKLSMENNNFLILDEPTNHLDIDSKEVLENALIEFDGTLLFVSHDRYFINRVAT
                     KVLEISDKGSTLYLGDYDYYLTKKAELEELARLNEEEVSASKTEIDVTSDYETQKANQ
                     KEFRKITRRVAEIEARLEVLENDENNINGLMLETNDIGKLSDLQKELESIQEEQLLLM
                     EEWENLNMRLDL"
     gene            26171..27139
                     /locus_tag="ACUBV3_000265"
     CDS             26171..27139
                     /locus_tag="ACUBV3_000265"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HHU3846801.1"
                     /translation="MVTLSKEQHLDMFLKMQRIRDVDMKFNKLVRRGFVQGMTHFSVG
                     EEAASVGAIQDLTDSDIIFSNHRGHGQTIAKGIDIGGMFAELAGKATGTSKGRGGSMH
                     LANLEKGNYGTNGIVGGGYALAVGAALTQQYEGTDNIVIAFSGDSATNEGSFHESVNL
                     AAVWNLPVIFFIINNRYGISTDITYSTKIPHLYMRADAYGIPGHYVEDGNDLMAVYEK
                     MHEVINYVRSGNGPAIVEVESYRWFGHSTADAGVYRTKEEVDSWKAKDPVKRYRAYLI
                     ENEIATEEELAAIEAQVIKEVEEGVKFAEESPFPDMSVAFEDVFVD"
     gene            27214..28212
                     /locus_tag="ACUBV3_000266"
     CDS             27214..28212
                     /locus_tag="ACUBV3_000266"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HHU3846802.1"
                     /translation="MSETKVMALREAINVAMSEEMRKDEKVFLMGEDVGVYGGDFGTS
                     VGMLEEFGAKRVRDTPISEAAIAGSAIGAAQTGLRPIVDLTFMDFVTIAMDAIVNQGA
                     KTNYMFGGGLSTPVTFRVASGSGIGSAAQHSQSLEAWLTHIPGLKVVAPGTVNESKAL
                     LKSSILDNNPVIFLEPKALYGKKEEVNMDPDFYIPLGKGDIKREGTDLTIVSYGRMLE
                     RVMQAAEEVAEEGINVEVVDPRTLIPLDKELIIDSVKKTGKLILVNDAYKTGGFTGEI
                     ATMVAESEAFDYLDHPIVRLASEDVPVPYSRVLEQGILPDVAKIKDAIYKVVNKGK"
     gene            28339..29727
                     /locus_tag="ACUBV3_000267"
     CDS             28339..29727
                     /locus_tag="ACUBV3_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - pyruvate decarboxylation to
                     acetyl-CoA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase"
                     /protein_id="HHU3846803.1"
                     /translation="MAVEIIMPKLGVDMQEGEILEWKKQVGDVVNEGDVLLEIMSDKT
                     NMEIEAEDSGVLLKITHGNGDVVPVTETIGCIGAEGEEVTEASSSENTSVEENATQVT
                     SEPEKVEETSEPSVPAATSGEKVRATPAARKLAREMSIDLALVSGTGANGRVHREDVE
                     SFKGAQPRITPLARRIAEDQGVDIAEITGSGIRGKIVKNDVLAAMSPQAAEAPVKTKA
                     TPTTEEKQLPEGVEVIKMSAMRKAISKGMTNSYLTAPSFTLNYDIDMTEMMALRKKLI
                     DPIMAKTGLKVSFTDLIGMAVVKTLMKPEHRYLNASLINDAQEIELHNFVNIGIAVGL
                     DDGLIVPVVHNADQMSLSDFVIASKDVIKKTQEGKLKSAEMSGSTFSITNLGMFGTKT
                     FNPIINQPNSAILGVGATIPTPTVVDGEIVARPIMAMCLTIDHRIVDGMNGAKFMVDL
                     KNLMENPFGLLI"
     gene            29787..31544
                     /gene="lpdA"
                     /locus_tag="ACUBV3_000268"
     CDS             29787..31544
                     /gene="lpdA"
                     /locus_tag="ACUBV3_000268"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     (NADH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HHU3846804.1"
                     /translation="MAFDVIMPKLGVDMQEGEILEWKKNEGDTVNEGDVLLEIMSDKT
                     NMEIEAEDTGVLLKIVHQAGDVVPVTEVIAYIGEEGEEVGTSSPSADATITAEDGQSV
                     SGPAAPSQETVAAATPKEELAADEYDIVVVGGGPAGYYAAIRGAQLGGKIAIVEKTEF
                     GGTCLNVGCIPTKTYLKNAEILDGLKVAAGRGINLASTNYAIDMDKTVAFKNSVVKTL
                     TGGVRGLLKANKVEIFNGLGQVNPDKSVVIGDKVIKGRNVVLATGSKVSRINIPGIES
                     PLVLTSDDILDLREIPKSLAVMGGGVVGIELGLVWASYGVDVTVIEMADRIIPAMDKE
                     VSLELQKILAKKGMKIKTSVGVSEIVEANNQLTLKLNNGEEVVADKALLSIGRVPQMN
                     GLENLEPELEMERGRIKVNAYQETSIPGIYAPGDVNGTRMLAHAAYRMGEVAAENALG
                     GNKRKAHLDFTPAAVYTHPEVAMVGMTEEQAREQYGDILVGKNSFTGNGRAIASNEAH
                     GFVKVIAEPKYKEILGVHIIGPAAAELINEASTIMENELTVYDVAQSIHGHPTFSEVM
                     YEAFLDVLGEAIHNPPKRK"
     gene            31642..32631
                     /locus_tag="ACUBV3_000269"
     CDS             31642..32631
                     /locus_tag="ACUBV3_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HHU3846805.1"
                     /translation="MKYIVNTSNDPAYNVALEAYAFQKLTDIDEIFILWINEPAIIIG
                     RHQNTIQEINKEFIDKNGIHVVRRLSGGGAVYHDLNNLNYTIISNNTQEGAFDFQTFS
                     KPVIDTLAKLGVKAEFTGRNDLEINGQKFAGNAQAYYKGRMMHHGCLLFDVDMSVLGQ
                     ALKVSKDKIESKGIKSVRARVTNIVDHLSDKITVQEFSDAILAQVKEEYPEMDEYVLS
                     DAELSEIQAMRDNQFATWDWTYGKAPEYTIERGVRYPAGKITTYANVENSTIKSVKIF
                     GDFFGVKPVDDIEKMLEGVRYDYKDVLAALKTVDTSQYFSRMTPEEITKAIVD"
BASE COUNT        10754 a         5295 c         6643 g        10131 t
ORIGIN      
        1 atatcttaat aagggattaa attaaagcta aaaaaactaa aaattgttat ttgaaaagga
       61 ttgtgtaaac catctcaaac tatttcatga aaacgtaatc aaatgaggaa attcattgac
      121 atgcacaaga aataggaata aaataacgga gtaaatattt tttaaaggag aattctatta
      181 tgaatttagc tattttagca cttggtttcg ccgtgatggg tgtttcaatc ggtgaaggaa
      241 ttttggttgc aaatattgcc aaatcagcgg ctcgtcagcc tgaaatgttt agcaaactgc
      301 aaacattgat gtttacaggt gtggccttca ttgaaggtac cttcttcgtt ctatttgcgt
      361 ttacattctt agtaagatag acgacaattt agaaaaaagg agggatgagg attggaatct
      421 acaagtaatc caaccgtatc ttttttaggc atcgattttg atttaaccat cctagctatg
      481 tctctcttga caattactat tatctttatc ttagtcttct gggctagtcg taagatgaca
      541 ataaaaccaa aaggtaagca gaatgtctta gaatatgtct atgagcttgt taataataca
      601 atctcacaaa atttaggaca ttatactaaa aattatagtt tattgatgtt tattttattc
      661 agttttgtct tcattgctaa caacttagga ttgatgacat cattgaaaac acatgagcat
      721 aatttttgga catctccgac cgctaatttt ggtgtagata ttacattatc attattagta
      781 gcatttattt gtcatattga aggtattcgc aaaaaaggaa ttggtggtta tctcaaaggt
      841 ttcttatcac caacaccagc aatgcttccg atgaatttat tggaagaagt tactaatgtt
      901 gcttcattag cattgcgtct ttttggtaat atcttttctg gtgaagttgt cactggatta
      961 cttttacaac ttgcagtttt atcaccattt acaggtccac tagcttttgc attgaatatc
     1021 gtttggacag cattttcaat gtttattggt ttcatccaag cgtatgtgtt cattatcttg
     1081 agctcaagtt atattggaca caaagttcat ggtgacgaag aagaataaga aaagagagga
     1141 gaaatatgtc aatacttatt aatagtacaa cgattggtga tatcattatc gtatctggct
     1201 ctgtgcttct cttatttatc ttaattaaga cttttgcatg gaaacaaatc acaggtattt
     1261 ttgaagcccg tgaacaaaag atagctaatg atattgatac tgctgaacag gcacgtcaac
     1321 aagctgaagc ttttgcaact aagcgtgagg aagaactatc taacgctaaa actgaagcta
     1381 atcaaatcat tgataatgct aaagagacag gtcttgcaaa aggtgatcaa attatttcag
     1441 aagcaaaaac tgaagctgat cgattaaaag aaaaagcaca tcaagatatt gcccaaaata
     1501 aggcagaagc actagcagat gttaagggtg aagtagcaga tttaacagtt ttattagctg
     1561 aaaaaattat ggtatcaaat cttgataaag aagctcagag taatctcatt gatagctata
     1621 ttaaaaagct aggagatgcc taatgaataa aaagacgcag gctctaattg agcaatattc
     1681 aaaaagcctt gttgaagttg ccattgaaca taaaattgtt gaagaaatcc aacaagaagt
     1741 agcagcttta attgatattt ttgaaacaag cgagcttgag ggtgtcctgt caagtcttgc
     1801 cgtttcacat gatgaaaaac aacattttgt gaagacattg caaacatcat gttcaactta
     1861 tcttgttaat ttccttgagg ttattgtaca aaatgaacgt gaagcacttc tatatcctat
     1921 tttgaaatca gttgatcagg aacttatcaa agtgaatggt caatatccaa ttcaaattac
     1981 aactgcagtt gctttgtctc cagaacaaaa agaacgtctt tttgatattg ctaaaactaa
     2041 attagcttta ccaaatggac agttagttga gcacattgac ccatctattg ttggtggatt
     2101 tgttgtcaat gcaaacaata aagtaattga tgcaagtgtt cgtaatcaat tacatcaatt
     2161 taaaatgaaa ttaaaataga aagtggtgtg acttttggca attaatgcac aagaaattag
     2221 cgctttaatt aaaaagcaaa ttgaagactt ccagccaaat tttgacgtca cagaaactgg
     2281 tattgttaca tatatcggtg atggtattgc tcgtgctcgt gggctagata atgctatgag
     2341 tggagaactt cttgaatttt caaatggtgc gtacggtatg gctcaaaacc ttgagtctaa
     2401 tgacgtgggt atcattattt tgggtgattt ctcagaaatt cgtgaaggtg atgtggtaaa
     2461 acgtactggt aaaattatgg aagtaccagt aggggaagct atgattggac gtgtcgtcaa
     2521 tccgcttggt caaccagttg atggactagg tgaaattgag acgacagcaa cacgcccagt
     2581 tgaaacacca gcacctggtg ttatgcaacg taaatcagtt tttgagcctc ttcaaacagg
     2641 tctcaaagcg attgatgctt tggttcctat cggacgtggg caacgcgaat tgattatcgg
     2701 tgaccgccaa actggtaaga cgtctgtagc gattgatgca atcttgaatc aaaaaggtca
     2761 agatatgatc tgtatctatg tggctatcgg tcaaaaagag tctacagtac gtacacaagt
     2821 tgaaactttg cgtaaatatg gtgcccttga ttacacaatc gttgtgacag cttcagcatc
     2881 acaaccatct ccattacttt tcattgctcc atatgcagga gtagcaatgg ctgaggaatt
     2941 catgtataac gggaaacacg ttttgattgt ttatgatgat ttatcaaaac aagccgtagc
     3001 ttatcgtgaa ctttcgcttc ttcttcgccg tcctccaggt cgcgaagctt acccagggga
     3061 tgttttctat cttcatagtc gtcttttgga acgttcagct aaagtatcag atgctttagg
     3121 tggaggttcg attacagcgc ttccgttcat tgaaacacaa gcaggtgata tctcagcata
     3181 tattgctaca aacgttattt caattactga cggccaaatt ttcttgcaag aaaatctctt
     3241 taactcaggt attcgtccgg cgattgatgc aggttcatca gtatctcgtg ttggtggtgc
     3301 agcgcaaatt aaagcgatga agagagttgc aggtacgctt cgtctagatc ttgcttctta
     3361 tcgcgaactt gaggcattca ctcaatttgg ttcagacctt gatgcagcaa cacaagcaaa
     3421 actaaatcgt ggtcgtcgca cagtggaagt gttaaaacaa ccattacaca aaccattgcc
     3481 agttgagaaa caagttgtga ttttatatgc tctaacacat ggattccttg atgatgtacc
     3541 tgtaaatgat attttagctt ttgaagaagc tttgtatgat tactttgatg ctcattatga
     3601 taatctcttc gaaacaatcc gcacaacaaa agatttacca gaagaagcag aattagatgc
     3661 tgctattcaa gcatttaaag atcaatcaca atttaaataa taggagggtt ctaatatggc
     3721 aggctctctt agtgaaatta aagacaagat tctttctaca gaaaaaacaa gtaagattac
     3781 tagtgcgatg cagatggtct catctgccaa gttggttaaa tctgaacaag ctgctagaga
     3841 ttttcaagtg tatgcctcta aaattcgtca aattactact aacctcttga agtcagactt
     3901 agtatctgga tcagataacc ctatgttagc ttcacgtccg gtgaagaaga caggttatat
     3961 tgttattact tctgataagg gtttagttgg tggttacaat tcaaaaatct taaaagcgat
     4021 gatggacact ataactgact atcatactga gaatgatgat tatgccatta tttcaattgg
     4081 tagtgttggt tctgattttt tcaaagcacg tggaatgaat gtttctttcg aattacgtgg
     4141 tttagaggac caaccaagtt ttgatcaagt tggtaaaatt attgctcaag cagttgaaat
     4201 gtacaaaaat gagttgttcg atgaattata tgtttgctac aatcaccatg ttaacagttt
     4261 aactagccaa gtcagaatgc aacaaatgct tcctatcaaa gaattggatg cagaagaggc
     4321 ttctgaggat agagtgatta ctggttttga acttgagcca aaccgtgaag ttatattgga
     4381 gcaattattg cctcaatata ctgagagttt gatttatgga gctattattg atgctaaaac
     4441 agcagagcat gcggctggta tgacagctat gcagactgca accgataatg ctaaaaatgt
     4501 tatcaatgat ttaactattc aatataaccg tgctcgtcaa gcagctatca cacaggaaat
     4561 tacagaaatt gttgcaggtg ctaatgcact tgaataatta attagtctat agaaaaaata
     4621 gtgaacaaag gtattttaaa tcctttgtat catatgataa ggagaaatac atgagctcag
     4681 gcaaaattgc tcaggtagtt ggacctgtcg ttgacgttgt atttgcaagt ggcgacaaac
     4741 ttcctgagat taataatgca ttgattgtct ataaaaatgg cgataagtca caaaaagtag
     4801 tacttgaagt tgctcttgaa cttggtgacg gcctcgttcg tacaatagct atggaatcaa
     4861 ctgatgggct tacacgtggt ttggaagtat tagatactgg tcgcgcaatt agtgtgccgg
     4921 ttggtaagga tactttgggt cgtgtcttca acgttcttgg agatgctatt gaccttgaag
     4981 agccttttgc agaagatgca gaacgtcaac caatccataa aaaagcacca tcgtttgatg
     5041 aattatcaac atcatcagaa atcttagaaa caggtattaa agttattgac ttattagcac
     5101 cttacttaaa aggtggtaaa gtcgggcttt tcggtggtgc gggtgttggt aaaaccgttc
     5161 ttattcaaga attaatccac aacatcgccc aagaacatgg tggtatttca gtatttactg
     5221 gtgtaggaga acgtactcgt gaagggaatg acctttattg ggaaatgaaa gaatctggcg
     5281 ttattgaaaa aacggctatg gtctttggtc aaatgaatga accaccagga gcacgtatgc
     5341 gtgtggcact tactggtctt acaatagctg aatacttccg tgatgtagaa ggacaagatg
     5401 tgcttctctt cattgataat atcttccgtt tcacacaagc ggggtcagaa gtgtcagcgc
     5461 ttttaggtcg tatgccttca gccgttggtt atcaaccaac acttgctaca gaaatgggac
     5521 aattacaaga gcgtatcact tcaactaaaa aaggttctgt tacatcaatc caagctattt
     5581 atgtgccagc agatgactat actgaccctg cgccagctac agcctttgct cacttagatt
     5641 caacaactaa ccttgaacgt aaattgacac aaatggggat ttatccagcc gttgacccat
     5701 tagcttcaag ttctcgtgca ctcacaccag aaatcgttgg agatgagcac tatgaagttg
     5761 caacagaagt acagcgtgtt cttcaacgtt accgtgagtt acaagatatc attgcgattt
     5821 taggtatgga tgaattatca gatgaggaaa aaacgttagt tggacgtgca cgtcgtatcc
     5881 aattcttcct ttctcaaaac ttcaatgttg cagaaacatt tactggtcaa ccaggttcat
     5941 atgttccggt tgaagaaact gttcgcggat tcaaagaaat cctagatggt aaacatgatc
     6001 aaattccaga agatgccttc cgtatggttg gtggtattga ggatgttatc gcaaaagcag
     6061 aaaaaatgaa ctattaatga ggtgatgcta tggcacaatt gactgttcaa gtagtaacat
     6121 cagatggtat tagatatgac catcacgcta gtctcattac tgttagaacg ccagatggtg
     6181 aaatgggaat tttaccaggt cacataaatt tgattgctcc ccttattgtt catcagatga
     6241 agattaatcg ttctcatcaa gaaggtgtag actgggtagc tgttaatggt ggtataattg
     6301 aagttaatga ggatcaggtg acaattgttg cggattcagc agaacgagca cgtgatatag
     6361 accttaaccg tgcagaacgg gctaaggaac gcgctgagcg tgcattagaa aaagctcaaa
     6421 caactcagaa tattgatgag atgcgtagag ctgaggttgc tcttcgtcgc gcaattaacc
     6481 gtattagcgt cggtaaaaag tagaaaaatg gtctaattag ttttgtgact aattaggctt
     6541 ttttagtgta taatagaagg catgaatatg atattaaata tagtaactat agttagtaat
     6601 tttattttta ttgccctttt ttatcaactt tttgtagatt tatttgactg gtctaaaatg
     6661 attaaaatgt cccctcaaaa catctcaaaa ttaaaagttt ttattttgtt gataagtata
     6721 gttggtggct atgtggttag tcatttttta ttggaagtga ttcagttgtg tcaatcaatc
     6781 ttttgggctc tccaataaag gaaatattag ctgaactatg atataatcaa cttactattg
     6841 ttataagaat ggagattcaa atggataaaa ttattgtgga aggtgggcaa acgcggcttc
     6901 aaggtcaagt tgttattgaa ggagctaaaa atgcagtttt acctctttta gcagccacaa
     6961 ttttaccaag ccaagggaaa acattattga ccaatgttcc tatcttgtct gatgtattta
     7021 caatgaacaa tgttgttcgt ggtttagata ttcaagttga ctttaattgc gataaaaaag
     7081 aaatcttagt ggatgcctca ggtgatattt tagatgttgc accttatgaa tttgttagtc
     7141 aaatgagagc ttctattgtt gttttgggac ctattttagc tagaaatggc catgctaagg
     7201 tttcaatgcc aggcggttgt acaattggta gccgaccaat tgaccttcat cttaaaggtt
     7261 tagaagctat gggagctacg attacacaaa atggtggtga tattactgcc caagctgaaa
     7321 agcttaaggg ggctaatata tacatggatt ttccctcagt cggtgccact caaaacttga
     7381 tgatggctgc tacacttgct tcaggcacaa caacgataga gaatgctgca cgtgaaccag
     7441 aaattgtaga ccttgcgcag ttactaaata aaatgggagc taaagtcaag ggtgctggta
     7501 cggaaacatt aacgatcatt ggtgttgatg ctttacatgg tacagaacat gatgttgtcc
     7561 aagatcgtat tgaagctgga acctttatgg tggcagctgc tatgacttct ggaaatgttc
     7621 tagtaaagga tgctatttgg gaacataacc gtcctttaat ctctaaattg atggagatgg
     7681 gtgttgaagt gtcagaggaa gaagatggta ttcgtgtaaa agctgatact aaaaaattga
     7741 agccagtgac tgtgaaaaca ctccctcatc caggatttcc tactgatatg caagctcaat
     7801 ttacagctct aatggcagta gttaatggcg aatcaactat gattgagaca gtttttgaaa
     7861 atcgttttca acatttggaa gaaatgcgtc gcatgggatt acaaacggaa attttacgag
     7921 atactgcaat gattcatggt gggagagcct tacaaggtgc accggtcatg tctactgact
     7981 tacgagctag tgcagcttta atcttggcag gaatggttgc gcaagggcaa acagtagttg
     8041 gccaattaac ccatttagac cgtggttatt atcaattcca tgaaaaatta gcggctcttg
     8101 gtgctaatat taaacgtgta agtgaggcgt aatatggcat ctagtggttg gaaatatgta
     8161 ctaaaacaaa tcggtctaat agtattagtc atactgttag ccttgctgtt tttagcggtt
     8221 ggattgatgt tgggttatag tgtttttggt gacggagagc atgcttactc tattttatcg
     8281 ctggataaat ggcaaaatat tattggaaaa tttttaggaa aatagaaaga gccactataa
     8341 gttatttgat gcttatagtg gtttcctttg agagttaatt ttagcagtag aataatacaa
     8401 tgacaaaaaa acaaaaacaa ataacagctt cgattgtaac tttgcttttt tcattactgg
     8461 caatagggtt aggtgaaagt aaatctttgc ctaatgatca cattttaaaa caggtgacag
     8521 atttaattat tagtaatgaa tctggcaaaa atacttccct taaaaatata tcaggaaccc
     8581 ctagtagaga gttatctgaa tctgttttga ctagcaatgt taaaaaacaa ttgggaacaa
     8641 atatagcttg gaaccagtca ggtgctttta tcattaatca aaataaaaca gatttgaatg
     8701 ctaaagtgtc aagtgcacca tatgctatta atgaaataaa gaaggttaat aaccaaattg
     8761 ttccaactaa agcaaatgcc ttattaacaa aagcaactcg tcaatatcga aatcgagaag
     8821 aaacagggaa tggtagaact tattggaaac cagctggatg gcatcagata aatggattaa
     8881 aagggagcta taaccacgct gtggacagag gccatttaat tggttatgct ttagtaggga
     8941 gtttaagagg atttgatgcc tccacaagta atcctaaaaa tattgccaca caagcagctt
     9001 gggcaaacca agctaatagc aaccaatcaa cagggcaaaa ttattatgaa actttagttc
     9061 gtaaagcatt ggatcgtcat aaaactgtta ggtatcgtgt gactttaatt tatgacagag
     9121 ataatctttt atcttcggga tcacatattg aggctaaatc ttcagatgga agtttagaat
     9181 ttaatgtttt tattcctaat gtacaatcgg gattattatt tgattatgca acaggaaaag
     9241 tgaagcagac aaagtaatct ttatctaata gtgcttgtta aagactgaaa aatttgataa
     9301 aatagtcctt aattgaatag aggtctgtga gactagtaaa ccaagggaag ctgcttaggg
     9361 agttcagccg tgactgcaag ctgaatcagt gaaagagggt ggaattcact cacaaatagg
     9421 actataatta aggtctgatt ggtcgatcag atagaaaacg gatggtaccg cgtgtcaacg
     9481 ctccgattaa gggggagtgg cacgcttttt ttgttttcaa agctacttat tttgacttgg
     9541 aggaaagatg gatttacaaa aacaattaga agagttgaaa atatcaactc aagaaaagct
     9601 taaagaaatg actgggaacc acactaaaga attacaagac ttacgtgtac aagttttagg
     9661 aaaaaaaggt tctctaacag aattactaaa aggtctgaaa gatttatcta atgatttacg
     9721 accagttgtt ggaaaacaag tcaatgaagt acgtgatatt ttaacaaaag cgtttgagga
     9781 acaagctaaa gtagtggaag ctgcgaagat tcaagcacag ttagagtctg agagtgtcga
     9841 cgttaccttg cctggtcgtc aaatgacatt aggtcaccgt catgtcttaa cacaaactag
     9901 cgaagagatt gaagatatct ttttaggtat gggattccaa gtggttgatg gttttgaagt
     9961 tgagaaagac tactataatt ttgaacgaat gaatttacca aaagatcatc cagcccgtga
    10021 tatgcaagat actttttata ttacagaaga aatcttgcta cgtactcata cttcgcctgt
    10081 tcaagcacga acaatggatc aacatgattt ttctaaaggg cctttaaaaa tgatttctcc
    10141 tggacgtgtt ttccgtcgtg atactgatga tgctactcat agccatcaat tccatcaaat
    10201 tgaaggctta gttgttggag aaaatatttc aatgggcgat ctcaaaggaa cacttcaact
    10261 gatttctcaa aaaatgtttg gagcagagcg taaaatccga cttcgtcctt catatttccc
    10321 attcacagaa ccttcagttg aagttgatgt ctcttgcttc aagtgcggag gtaagggatg
    10381 taatgtctgc aaacagacag gttggattga gatattgggt gctggtatgg ttcacccaag
    10441 tgttttggaa atgtctggta ttgattctga gaaatactcc ggttttgcct ttggcttagg
    10501 tcaagaacgt attgcaatgt tacgctacgg tatcaatgac attcgtggct tctatcaagg
    10561 tgatgttcgc ttcacagatc aattttaaga ttttagatag caagtagcat cattggtgtg
    10621 atgataaatt tatgttaacg ctattacctt actcaatagg aggttttcta atgtctcaaa
    10681 ttacttttaa aaaggttggc cttgataatg tgaatatttt acagaatctt gctatagaaa
    10741 cctttcggca gacgttttct catgataata gtgaggaaca acttcaagca tttttcaatg
    10801 agtcttacac tttaccagta ttaaaatcgg aaattaccca tgctgaatct gatacttatt
    10861 ttgtttatct tgatacagat ttagtgggtt atttaaaagt aaactgggga agtcagcaaa
    10921 ctgaaaaaaa cttgataaag catttgaaat tcaaaggatt tatcttctag atgcctacca
    10981 agggaaaggt ataggtaagg ctactttcga atttgctttg gatttagctt ataaatcagg
    11041 cttagattgg gcttggttag gtgtttggga gtttaatcat aaagctcagg cattttacgc
    11101 taaatacgga tttgaaaaat tttctgagca ccaattttca gtaggtgata aagtggatac
    11161 ggattggtta ttaagaaaaa gtttacacta aaaactacaa agcacttgta taaaaataaa
    11221 agaaaataaa tagagagaaa ttacatgtta gtatcgtata aatggttaaa agagttagtg
    11281 gatgttgatg tcacaacagc agaactagct gagaaaatgt caaccactgg tattgaggtt
    11341 gaaggtgtgg agacaccagc ggaaggttta tcaaaattag ttgtaggtca tattgtttcg
    11401 tgtgaagatg ttccagatac ccacttacat ttgtgtcaag ttgatacagg ggacgatgaa
    11461 cttcgtcaag ttgtctgtgg tgctccaaat gtcaagactg gtattaatgt tatcgtagcg
    11521 gttcctggtg ctcgtattgc agataactat aagattaaaa aaggtaaaat ccgtggtatg
    11581 gagtcacttg gaatgatttg ttcacttcaa gagttgggct tatcggaaag tatcattcca
    11641 aaagagtttt ctgatggtat ccaaatttta cctgagggag ctatacctgg tgatagtatt
    11701 ttctcttatc ttgacttaga tgatgaaatt attgaattat caattacacc aaaccgtgca
    11761 gatgctcttt caatgcgtgg tgttgcccat gaggtagcag caatttatgg gaaaaaagtt
    11821 cattttgaag aaaaaaatct cattgaagaa gcagaaagag cagctgataa gatttctgtt
    11881 gtcattgaat cagataaagt tttatcttac tcggctcgta ttgttaagaa tgtaacagtt
    11941 gcaccaagtc cacaatggct tcaaaataaa ctgatgaatg ctggtatacg tccaattaat
    12001 aatgttgttg atgtgacaaa ctatgttctt ttgacttatg gtcaaccaat gcatgctttt
    12061 gattttgata aatttgatgg gacaactata gtagcacgta atgctgagaa tggtgaaaag
    12121 ttaattactc ttgatggtga agagcgtgac ttaatagctg atgacttggt cattgcggta
    12181 aatgatcaac cagttgcttt ggcaggcgtt atgggaggac aatcaactga aattgatagt
    12241 tcttctaaga cagtggtcct tgaagcggca gtatttaatg gaacaagtat ccgcaaaaca
    12301 agtggaagac tcaaccttcg ttcagagtca tcttctcgct ttgaaaaagg tatcaattat
    12361 gatacggttt cagaggctat ggattttgcg gctgcaatgt tacaagaatt ggcaggcagt
    12421 caagttcttt ctgggcaagt tacagaagga gttcttccaa ctgagccggt agaggtatca
    12481 acgactcttg gttacgttaa tactcgtttg ggaacagaac ttacctatac tgatattgaa
    12541 gaagtatttg aaaaactcgg ctttgctatt tctggttcag aagtgaaatt tactgtgttg
    12601 gtaccgcgtc gtagatggga tattgccatt caagctgact tagttgaaga gattgcccgt
    12661 atttatggtt acgaaaaatt accaacaaca ttgccagaag ccggagcgac tgcaggagaa
    12721 ttaacgagta tgcaacgctt acgtcgtaga gtccgtacgg ttgctgaagg agtaggatta
    12781 tctgaaatta tcacctatgc tttaacgact ccagaaaaag cagtgcagtt ctcaactcaa
    12841 gcaactaata taactgaact tatgtggccg atgactgttg atcgctcagc acttcgccaa
    12901 aatgttgttt caggaatgct tgatacaatt gcttataacg ttgctcgcaa aaacagtaac
    12961 cttgcagttt atgagatagg aaaagttttt gaacaaactg gtaatccaaa agaagattta
    13021 ccaactgaag tagagacgtt tacttttgcc ttaacaggtc tagtagagga aaaagatttc
    13081 caaacaaaag ccaagccagt tgatttcttc tacgccaaag gtattgtaga ggcgctcttt
    13141 attaaattaa aattagatgt tacttttgta gcacaaaaag gtttagcaag tatgcaccca
    13201 ggtcgtacag caactatctt attagatggg aaagagattg gttttgttgg tcaagtacac
    13261 ccacaaactg caaaacaata tgatattcct gaaacttatg ttgctgaaat caatctatca
    13321 actattgaaa gtcaaatgaa ccaagcgttg atttttgaag atattacgaa gtatccgtct
    13381 gtatctcgcg atattgcatt attacttgca gaatctgtta gtcatcatga tattgtgtta
    13441 gcaattgagt catcaggggt aaaacgtcta acagcgatta aactgtttga tgtctacgct
    13501 ggaaataata ttgcagaagg gtataaatca atggcttata gcttaacttt ccaaaaccct
    13561 aatgacaatc taacggacga agaagtggct aaatatatgg aaaaaatcac taaatcactc
    13621 gttgagaaag ttaatgcaga aataagataa tttatatctt acgttgaaaa ataaataaaa
    13681 aacagcatca attaagatgc tgttttttta tttaaaattt atagatgttc tgcggagaaa
    13741 gtatcaccgt gttggatgtc accatattga aagcctttgt taaaccagcg ttggcgttgt
    13801 tgagctgttc catgggtaaa gctatcaggc actaattttc cgtaggtttc tttctgaagg
    13861 gtatcgtctc cgacggcgtg ggcagcattc atggcctctt caaagtctcc ttgttctaag
    13921 agattttttc ccctgatgta gtgagcccat acccctgcat aataatctgc ttgaagttct
    13981 agccgaacat ttaaagcatt tgcttctttc ttagtaagtc cgtgtcgcat tctattatac
    14041 ttatccataa tgcctaactc tgtttgaatg tggtgaccaa cttcatgggc gatgacgtag
    14101 gccatagcaa aatcaccagt agcaccatat ttatgtgata attcattgta aaaagaaata
    14161 tcaagataga tttttttatc tgctgaacaa taaaatggtt ctgaagcaga ttcacctata
    14221 ccacaacctg tttgaatcga attggtgtaa agaacaagtt ttggttcctt ataatttcca
    14281 aaaccttggg tttggaattc ttgtgaccag aaatcctcag ttgatccaag gactttatta
    14341 acgaaatcga tttgttctct cgttgcgcta ttatcaacag aacgtgagac attctgagat
    14401 tggtaactag aaggtgagga tgagtcatta aaaatgctgg ttagtccccc tccgccaagt
    14461 agcagtaaga tgattaaaac cacaagcttg gttttccaac tccctcgcat caataaaagt
    14521 tgaagaatcg gtaatccact tcctccgcta gagaatgaac ctccgctact ggagcgacga
    14581 tcttcaacat tgtcgctttt tcttaggtca tctattttca taagtatctc caaagtgttt
    14641 tataggctca ttataaacta ttttaataag aaatgcttga atttttaact aaatatcttc
    14701 ccctagattt gaagctattt ttggtataat attaataaac tgtaaagaat aagttgaaga
    14761 aggtttattt ctcagttgac ttataagata ggagattcat gaaattactt tatactgata
    14821 ttaatcatga tatgacagaa atcttagtca atcaggccgc gcatgctgct gaagctggat
    14881 ggcgtatttt ttatattgct ccaaactctt tatcttttga aaaggagcgt gcagtattag
    14941 agacccttcc tcaagaagcc tcttttgcta ttacaattac tcgctttgct caattggcac
    15001 gttattttac tttaaaccag cctaatcaaa aagaaagttt aaacgatatt ggattagcaa
    15061 tgatctttta tcgtgcttta gcaagttttg aagatggaca attaaaagtt tttggacgat
    15121 tgaagcaaga tgcttccttt atcagtcaat tggtagacct atacaaagaa ttgcagacag
    15181 caaatctgag tattttagat ttaaaatatt tacattctcc tgaaaaattt gaagatctat
    15241 tagctatttt tctagtggtt tcagatcttt taagagaagg agaatacgac aatcaatcta
    15301 aaattgcctt ttttacagaa caagtaaggt caggacagct agatgtggat ttaaagaaca
    15361 ctatccttat agtcgatggt tttacccgtt tctcagctga agaagaagct ttaattaaaa
    15421 gtctgagttc acgttgtcaa gagattatca tcggagctta tgctagccaa aaagcgtata
    15481 agactaactt taccaatgga aatatttatt ctgcaggggt tgatttttta agatatctag
    15541 caacgacatt tcaaacaaaa ccagaattta tcttgtctaa gtgggaaagt aagtctggtt
    15601 ttgaaatgat ttcgaaaaat atcgaaggta aacatgattt tacaaatagt agtcacattt
    15661 tagacgacac tgctaaagat tgtataacaa tttgggagtg cattaatcaa aaagatgaag
    15721 tggaacatgt ggctagagct atacgtcaga aactttatca gggatatcgc tacaaagata
    15781 ttttagtttt actaggagat gtagatagtt ataaactaca attaagtaag atttttgaac
    15841 agtatgatat tccttattat ttcggtaaag ctgaaacaat ggctgcgcac cctttagttc
    15901 attttatgga ttccttgtcg cgaataaaac gttatcgatt tagagctgaa gatgttttaa
    15961 atctttttaa gacaggtatt tatggagaga ttggtcaaga tgatttagat tattttgaag
    16021 cttatatctc atatgcagat attaaaggtc ctaagaagtt ctttacagat tttgttgtgg
    16081 gggctaaaaa atttgattta gggcgtctta acactattcg tcagtccctc cttactccac
    16141 tggaaagttt tgttaaaacg aaaaaacaag acggtataaa aacactcaac caattcatgt
    16201 ttttccttac tcaggtaggt ttgagtgaca atttatcgcg attagttggt caaatgtctg
    16261 aaaatgaaca agaaaaacat caagaagttt ggaaaacatt tacagatatt ttagagcagt
    16321 ttcaaacaat atttggtcaa gaaaaattaa atttagacga atttttatcg ttattaaatt
    16381 caggtatgat gcaagccgag tatcgtatgg tccctgctac tgttgatgtg gttactgtta
    16441 agtcttacga cttggtagaa ccacatagta atcaatttgt ctatgcccta ggcatgacac
    16501 aatcacattt tccaaagatt gctcaaaaca aatctttaat atctgatatt gaacgtcagc
    16561 ttataaatga tgccaatgac actgatggtc attttgacat tatgacgcga gaaaatttga
    16621 agaaaaacca ttttgctgct ctctctcttt ttaatgctgc taaacaagca ttagtgttga
    16681 ctataccaca attattgaat gagtctgaag atcagatgtc accatatctt gtcgaactaa
    16741 gagatatagg tgttcctttt aatcataaag gacgccaatc gttaaaagaa gaagctgata
    16801 atattgggaa ttacaaagct ttattatcgc gtgttgttga cttgtatcgc tctgctattg
    16861 acaaagagat gactaaagaa gaacaaactt tttggtcagt tgctgttcgt tatttgcgtc
    16921 gtcaattaac ttctaaaggg atcgagatac caataattac agatagtctt gatactgtga
    16981 cagtttcatc agatgtgatg accagacgct tcccagaaga tcacccactt aaattatcct
    17041 catctgcttt gacaactttt tataataatc aatataagta ttttttacaa tatgtgttag
    17101 gtttggaaga acaagactca attcatccag atatgcggca ccacggaacg tatcttcatc
    17161 gtgtttttga aattctgatg aaaaaccaag ggatagaatc ttttgaagag aaactcaact
    17221 ctgcaattaa taaaaccaat caagaagatg ttttcaaatc cttgtattca gaagatgcag
    17281 aaagtcgtta ttctttagaa attttagaag atatcgcgcg tgcaactgca accatattac
    17341 gacaagatag tcagatgact gttgaaagtg aagaagaacg atttgaacta atgattgata
    17401 atactataaa aattaacggt attattgacc gtatcgatcg tttatctgat ggttctttgg
    17461 gtgtcgtgga ctataaatca agcgctcaaa aattcgatat ccaaaaattt tataacggtc
    17521 taagtccaca attagtcacc tacattgatg ctatcagtcg tgacaaggaa gttgaacaaa
    17581 aaccaccgat ttttggagct atgtacttac atatgcaaga acctagacaa gacttgtcta
    17641 aaattaaaaa tttagatgat ttagtaacaa aaaatcatca agctttaact tataaaggat
    17701 tattttcaga agccgaaaaa gaattcttag caaatgggaa ataccatctc aaagattccc
    17761 tttattcaga ggctgaaatt gctattttac aagcacataa tcaatcgctt tacaaaaaag
    17821 catctgagac tattaagtca ggtaagtttt tgatcaatcc atatacagaa gatgctaaaa
    17881 cagttgacgg tgatcagttt aagagtatta cggggttcga ggcggatagg catatggcac
    17941 gtgccagagc gctttataaa ttacctgcta aggaaaaacg tcaaggtttc ttaacattaa
    18001 tgcagcaaga ggaggaaaat gatgaccttt aagcctttct taaatccaga agatattgct
    18061 gttattcaga cagaagaaaa aaattctgat aaaaaacaaa agcgaacgcc tgaacaaatt
    18121 gaagcaattt atacttttgg taacaatgtc ctggtatcag cttcggctgg ttctggaaag
    18181 acttttgtaa tggttgagcg gattttagat aaacttttaa gaggggttcc gattgagtct
    18241 ttgtttatct caacatttac agtaaaagcg gcaggtgagc tcaaagaacg tttagaaaag
    18301 aaaataaacg agtctttgaa atccgcagaa tcagatgatt taaaacagtt tctcacccag
    18361 caacttgtcg gcattcaaac agctgatatt ggtactatgg atgcttttac gcaaaaaata
    18421 gttaaccaat atggttatac tttgggcata tcacctatat ttaggatttt acaagataaa
    18481 aatgagcaag acgtcataaa gaatgaggtc tatgctgatt tattttctga ctacatgact
    18541 gggaagaatg cagcttcatt cataaaatta gtcaaaaatt tctcaggtaa tcgtaaagat
    18601 agcaaagctt ttcgagaaat ggtttataaa gtttatgctt ttagccagtc catcgataac
    18661 cccaagcgct ggatgcaaac agtttttcta aaaggtgcac aaacttacac tgattttgaa
    18721 gctattccag atcaagaagt ttcaagcctt ttaaatgtaa tgcaaacaac tgctaatcaa
    18781 ttacgtgatt taacggatca agaagattat aagcaattaa ctgctaaagg ggttccgaca
    18841 gcaaattaca aaaaacacct taaaataatt gaaaatttag tccattggtc tcaagacttt
    18901 aatttgctgt atggaaaaaa aggattggct aatttggcac gtgatatcac taatgtgatt
    18961 ccttcaggca atgatgttac agttgctggc gttaaatatc ctatctttaa acaattgcat
    19021 aatcgtatcg ttggacttaa gcacttagaa gttattttta agtatcaagg agaatctctt
    19081 ttcttgttag aattattgca atcctttgtt ttagatttct cagaacaata tttacaggag
    19141 aagattcaag aaaatgcttt tgaattttct gatattgccc attttgcaat tcaaatattg
    19201 gaagaaaatc atgatattag gcaaatttat caagataagt atcatgaagt aatggttgat
    19261 gagtatcagg acaataacca tacccaagag agaatattag aattattgtc taatggacat
    19321 aatcgtttca tggttggaga cataaaacaa tcaatttacc gttttcgtca ggctgatcca
    19381 caaattttta atgataaata caaagcttac caagacaatc caagtcaagg gaaattaatt
    19441 attctgaaag agaattttcg gagtcaatca gaggttttag acagtacaaa tagtgtgttt
    19501 acacatctta tggatgaaga agtaggagac attctttatg atgaaagtca ccaattaaaa
    19561 gctggtagtc caagacagca agaaagacat ccgaataata aaacacaagt tttactatta
    19621 gatactgatg aagatgatat agatgacagt gatagtcaac aatatgatat tagcccagct
    19681 gaagcaaagt tagtcgcaaa agaaattatt cgattgcata aagaagagaa tgttcccttt
    19741 caagatatca ccctcttagt ttcttcaagg acaaggaatg atggtatctt acaaactttt
    19801 gaccgttatg gtattccttt ggtaactgac ggtggggagc aaaattactt aaaatcagtc
    19861 gaagtgatgg taatgcttga tactttgcga tcaattgata atcccttaaa tgattatgct
    19921 ctagttgctc ttttgagatc tcccatgttt ggctttaatg aagatgattt aacaagaatt
    19981 gctattcatg atgtcaaaat ggctttttat cataaagtga aattgtctta tcataaagaa
    20041 ggtcatcata gtgatttaat tactccagaa ttatcttcta aaatagatta ctttatgaag
    20101 acattccaga cttggcgtga ttttgctaaa tggcattcgc tttatgattt aatatggaaa
    20161 atatataatg accgttttta ttatgattat gtaggagctc tgcctaaagc tgagcaacgt
    20221 caagccaatc tatatgcctt ggcacttcga gcaaatcagt ttgaaaaaac aggtttcaag
    20281 ggtttatcac gttttattcg aatgattgat aaagttttag aaaatgaaaa tgatttggca
    20341 gatgttgagg tggcattacc gcaaaatgct gttaacctaa tgactataca caaatcaaag
    20401 ggtcttgagt ttaagtatgt atttatttta aatattgata agaaattttc aatggtagac
    20461 ataacttccc ctcttatttt aagccgaaat caaggaattg gtattaaata tgttgctgat
    20521 atgcgacatg aactcgaaga agagatactg cctgcagtaa aggttagcat ggaaacactt
    20581 ccttaccaac ttaataaaag agagttacgc ttagctactt tatcagagca aatgcgttta
    20641 ctttacgtag ctatgacacg tgcagagaaa aaattatatc tggttggtaa ggctagtcaa
    20701 accaaatggg ctgatcatta tgacttagta tctgaaaata attatctccc attagctagt
    20761 cgtgagactt ttgtgacctt tcaagattgg ttactggctg ttcatgaaac ctataagaaa
    20821 caagagttgt tttatgacat caacttcgta tcattagagg aattaacaga tcaccacata
    20881 gggatggtta atcctagtct acctttcaat cccgataata aggcagagaa tcgtcagtct
    20941 gaagatatcg tgcacgcgat tagcgtgtta gaatctgttg aacagattaa tcaaacttat
    21001 aaagcggcta ttgagttacc gacagtaaga acaccaagtc aagtcaaaaa attctatgag
    21061 cctattctag atatagaagg cgttgatgtg atggaaacta ttactaaaac atcagtagat
    21121 tttaagttac ctgatttttc tacaagtaaa aagcaagacc cagcagcttt aggatctgct
    21181 gtacatgaat taatgcaacg catagagatg tcatctcatg tcaaaatgga agatattcaa
    21241 aaagccttga cagaagtgaa tgctgaaaca agtatcaaag cggctattca gattgaaaaa
    21301 atcaactatt ttttccaaga aaactcactc ggtaaatata tacaagaaga agtagaacat
    21361 ctacatcgag aggcaccttt tgccatgtta aaagaagatc cggagagtgg tgagaaattc
    21421 gttgtacgtg gtattattga tgggtatcta ctattagaaa atcggataat tctatttgat
    21481 tataagactg ataaatttgt caatcctctg gaattaaaag aacgctatca ggggcaaatg
    21541 gctttatatg cagaagcttt aaaaaaatct tacgagatag aaaggataga caaatactta
    21601 attcttttag gtggtaaaca gctagaagtg gtaaaaatgg attaaagaaa ggaaaaaatg
    21661 atagtcgaac aaaaatttgg aaatggtttt acttggatta acattgaagc agagcaattg
    21721 cgaacagaaa catcggaaat tcaagcaaaa tatttggata gtgagattat tacatatgct
    21781 ctggatgatt atgaacgagc ctttatggaa tgttctcata ttaaaggaaa agaagtcttg
    21841 acgattattt ttaatactat tgatctaaag cagaaagaaa gttattatga gaccgttccg
    21901 atgacctttt gcttatcaca cgaccgtttg ataacggtga cacggtctcg aaatagttat
    21961 atgctagaac tattgcaaaa atatctagac cgtaatccag atgtctctcc taaaaaattt
    22021 ttatttgcag ctttaacttt gattacaaaa caatatttta atgttgtatc gaaaattgat
    22081 agagaaaagg atattctcaa ccgtcaattg agagaacaaa caaccaataa gcgcttatta
    22141 gccatgagtg acctagagac aggctccgtt tatcttttga cagctgccaa tcaaaatgca
    22201 ttggtattag agcaattaga tgttcatcct agtcagcgat ttaattctga agtggaaaaa
    22261 gaacagttat cagatgctct cattgaagcg catcagttag tcagtatgac acaattaaac
    22321 agtcaggtac tttcacaatt atcatctact tttaataatg tcttaaataa taacttgaac
    22381 gaaaacttaa caggtttgaa tatcatttcg attaatttag cgattattgc agcaattact
    22441 ggcttttttg ggatgaatat tccattacct cttacagaat cacggagttc atggttaatt
    22501 gttattgcta cttcggtctt actttgggtt attattgctc aaattttaaa acgagttttt
    22561 aacttaagaa aataaaaatg ttcagtgcgt aagcaccgaa cattttttat ttacctaagc
    22621 agaattggct gaagagctga gttattagct catctggagc tgcatcccca gtgatttctc
    22681 caagtatttc ccaagttcta gtcatatcga cttgtaagag atcaacaggc attcctaatt
    22741 ccaagccctc attgacagct tttaaactat caacggcttt ttcaattagt gagatatggc
    22801 gagcattcga tagataagta gcgtcatgtt ctaccatacc agcattgtca aagaaaatat
    22861 cgttaatacg ttcttcgatt ttatcgatgt tttcattttc taaaaccgaa attggaataa
    22921 cattttcagg taattcgtta acttctattt tttgaggaag atccgtttta ttgagtaata
    22981 cgatacgatt actttcttta cttagttcta gaagggagcg gtcttgtaat gttagtggtt
    23041 cggaactatt gagaaccaac aacacaaggt cggcttcttc taaagccttc tttgaacgtt
    23101 caacaccaat tttttcaacg atatcatctg tatcacggat accagcagtg tcaactagtt
    23161 ttaaaggaac tcctttaata ttgacgtatt cttcaataac atcacgagtt gttccttcaa
    23221 tatctgtcac aatcgccttt tcttcacgta agaggttatt tagtaagctg gatttaccga
    23281 catttggacg accaatgatt gctgttgaaa gtccttctcg aagaattttt ccacgtcggg
    23341 cagttcgaag taaattttcc ataagtgctt gaaactcttg tgttttttca cgcatcagtg
    23401 ttgtagtcat ctcttcaacg tcatcatatt cagggtaatc gatattaact tcaacttggg
    23461 ctagagtatt tagaatttct tgtcgagtgt tattgattag tgtctttaaa gaaccatcta
    23521 attgcttaac ggcaatatcc attgctttat ctgttttagc tctaataaga tccataacag
    23581 cttcagcctg agtcaagtca actcgaccgt ttaaaaaagc acgtttagta aattctcctg
    23641 gttcagccat tcgtgctcca tgtcgtaaaa ttaactgaag aatttcgtta gtaactgcaa
    23701 ttcctccatg cgtattaatt tcaataacat cttctcttgt gaatgttttt ggagcaagca
    23761 taacgcctaa cataacttca tccaagattt catttttatc tgggtccaca atatgaccat
    23821 agtttaaggt atgggattgg atagcggata gatcttttcc acgataaatt tttgatgcta
    23881 tctttaaggc atctgtacca gatatacgga caataccaat agctccctca ccgagaggag
    23941 ttgaaatagc tgcgatggta tcaaattctt ttgtaataga cataaagtct ctctctttct
    24001 tgggaattac taacattcat tgtaacctaa agccctaaaa ggtgcaatca tttgattaaa
    24061 agagaagatt tccataatgg ttttatttga aggtatgcta taataaaact atgattatac
    24121 tacaaggaaa caaaattgag cgctcatttt caggtgatgt gctttttgat aatattaata
    24181 ttcaagttga tcaacgtgat cgtattgctc ttgttggacg taatggtgct ggcaaatcaa
    24241 cgcttcttaa aattttagtt ggtgaagagg ctccgacaaa aggagaaata aataaaaagc
    24301 gtgatttgag tttgtcctac ttggcacaag atagtcgttt tcaatctgaa aatacaattt
    24361 tccaagaaat gttacaagtt tttgattcat taagagaagt agagaagcgc ttacgtgaat
    24421 tggaattaca aatgggacaa gtttcaggaa gtgacctgga acaattgatg aaaacatacg
    24481 atattttgtc agaagagttt cgtgaaaaag gtggctttac ctatgaatca gatataaagg
    24541 ctattttaaa tggctttaag tttaacagtg atatgtggga aatgcctatt agtgaattgt
    24601 ctggtgggca aaatacacga ttagcgcttg caaaaatgct acttgaaaag ccagaattac
    24661 ttgttttaga tgaaccaacg aaccatttag acattgatac gattgcttgg ttagaaaatt
    24721 atcttgtaaa ttatcaagga gcccttatta ttgtcagcca tgaccgctat tttttagaca
    24781 aggtagctac agtaacttat gatttaacga cacattctct agaccgctat gtaggcaatt
    24841 attcgaaatt tatggatctt aaggcagaga aaatagcgac tgaagaaaag aattttgaaa
    24901 agcaacaaaa agaaattgct aagttagaag attttgtaca acgtaacatt gttagggctt
    24961 caaccactaa acgagcacaa gctcgtcgta aacaattaga aaagatggaa cgactcgaca
    25021 aaccgaatgt agagcaaaaa tcagctaata tgacttttca tgctgggaaa gtctcaggga
    25081 atgttgtttt aacacttgag aatgcagcta ttggttatga aggtgtttct ttatcggagc
    25141 caatcgattt ggacgtgaag aaatttgatg ctattgcaat tgtaggtcca aatggtatcg
    25201 ggaaatcaac tttaatcaaa tcgcttgttg ggcaaattcc ttttatcaaa ggagaagcaa
    25261 aattaggagc aaatgtcgaa actgggtatt acgatcaaag tcaaagtaat ttaacaaaaa
    25321 cgaatactgt tttagatgag ttatggaatg ctttttcaac aactccagaa gttgaaatta
    25381 gaaaccgttt aggagctttt cttttttcag gtgatgatgt taaaaaatca gtttcaatgc
    25441 tatccggtgg tgaacgagcg cgtttgctat tggcaaaatt atctatggaa aataataatt
    25501 tcctcatatt agatgagcca acaaaccact tggatattga tagtaaagaa gtcttagaaa
    25561 atgcactcat cgaatttgat ggcacattac tttttgttag tcatgatcgc tattttatta
    25621 atcgtgtggc aactaaggtt ctcgaaattt ctgataaggg aagtactctc tatctaggag
    25681 attatgatta ttacctcact aagaaagccg aactagagga attagcccgt ttaaatgaag
    25741 aagaagtgag tgcttcaaaa acagaaatcg acgttacatc agattatgag acacaaaaag
    25801 caaatcaaaa ggaatttagg aaaattaccc gtcgcgttgc agaaattgag gcacgtttgg
    25861 aagttcttga aaatgatgaa aacaatatca atggtttaat gttagaaacc aatgacatcg
    25921 gaaaactctc tgatttacaa aaagagctgg aatcaatcca agaagaacaa ttgctattaa
    25981 tggaagaatg ggaaaatcta aatatgagac tagatttgtg agaaaatttc aaaaacaata
    26041 gtttgtgaaa aaaagtacat tttaaattga gatgcctttt acgaaaacaa agaaagcggt
    26101 tcaaattctt gcatcttgtg aaaaaaactt atacaatgat taagtaataa agattttttt
    26161 ggaggttaga atggtaactc tatctaaaga acaacacttg gatatgtttc taaaaatgca
    26221 acgtattcgt gatgttgata tgaaatttaa caaattggtt cgtcgtggtt tcgtccaagg
    26281 gatgactcac ttctctgttg gtgaagaagc tgcttcagta ggtgctatcc aagatttaac
    26341 agattcagat atcattttct caaaccaccg tggacacgga caaactattg caaaaggtat
    26401 tgatattggt ggaatgtttg ctgaacttgc tggtaaagca acaggaacat caaaaggtcg
    26461 tggaggctca atgcacttag caaaccttga aaaaggtaac tatggaacaa acggtatcgt
    26521 cggtggagga tatgcgcttg cggtaggtgc tgcccttaca cagcaatatg aaggaactga
    26581 taacattgtt atcgcctttt cgggtgactc agcgactaac gaaggctcat tccatgagtc
    26641 agttaactta gctgctgttt ggaacttacc agttatcttc tttatcatca acaaccgtta
    26701 tggtatctca acagacatca catactcaac taaaattcct cacctttaca tgcgtgctga
    26761 tgcgtacggt attccagggc attatgttga agatggtaat gatcttatgg ctgtttacga
    26821 gaaaatgcat gaagttatca attacgtgcg ttcaggaaat ggaccagcta ttgtagaggt
    26881 tgaatcatac cgttggtttg gtcattcaac tgctgatgct ggggtatacc gtactaaaga
    26941 agaagttgat tcatggaaag ctaaagatcc agtaaaacgc taccgtgcat acttaattga
    27001 aaatgaaatc gctactgaag aagagcttgc agctattgaa gcacaagtga ttaaagaagt
    27061 tgaagaaggg gttaaattcg ctgaagaaag tccattccct gatatgtctg ttgcatttga
    27121 agacgttttt gtggactaaa ataacctaaa gtagatgaga tttatctcgt tctattattc
    27181 aattgattaa aaaacggata ttcggaggaa ataatgagcg aaactaaagt tatggctttg
    27241 cgcgaagcga ttaatgtcgc tatgagcgaa gaaatgcgca aagatgaaaa agtattttta
    27301 atgggtgaag atgtaggtgt atatggagga gacttcggta catctgttgg tatgttagaa
    27361 gagtttggtg ctaagcgcgt tcgcgacaca ccaatctcag aggctgcgat tgcaggttca
    27421 gctatcggtg ctgcacaaac aggtttgcgt cctatcgttg atttgacatt catggatttc
    27481 gtaacaatcg caatggatgc tatcgttaac caaggtgcta aaacgaatta tatgtttggt
    27541 ggaggtctat caactccagt tactttccgt gtggcatcag ggtcaggtat tggttcggct
    27601 gcacaacact cacaatcatt agaagcttgg ttaacacaca ttccaggtct aaaagttgtt
    27661 gcacctggta ctgttaatga gtcaaaagca cttttgaaat cttctattct tgacaataac
    27721 ccagttatct tcttagagcc aaaagctctc tatggtaaga aagaagaagt taatatggat
    27781 cctgatttct atattccact tggtaaaggg gatattaagc gtgaaggtac tgacttaaca
    27841 attgtttctt atggccgtat gcttgagcgt gttatgcaag ctgctgaaga agttgctgag
    27901 gaaggtatta acgttgaggt tgttgatcca cgtacactta ttccgcttga taaagaatta
    27961 attatcgatt ctgttaagaa aactggtaaa ttgatcttgg ttaacgatgc ttataaaact
    28021 ggtggtttca ctggtgaaat tgcaactatg gttgctgaaa gtgaagcatt tgattacctt
    28081 gatcatccta tcgtgcgtct tgcaagtgaa gatgttccag taccgtattc acgtgtactt
    28141 gaacaaggaa tcttacctga tgtagcaaaa attaaagatg ctatctacaa agtagttaac
    28201 aaaggtaaat aattcataaa acaaagaggg actgaggaat gagtgaaaac ttagcctctt
    28261 tccttgttta taggttttaa tttatatcgt tagatgaaac atatatttga cgatatagtt
    28321 tagtgtgaaa ggaaaactat ggctgtcgaa attattatgc ctaaactcgg ggttgacatg
    28381 caagaaggcg aaatccttga gtggaaaaaa caggtaggtg atgttgttaa tgaaggtgac
    28441 gtcctacttg aaattatgtc agataaaaca aatatggaga ttgaagcaga agactcaggt
    28501 gttttactaa aaatcacaca tgggaatgga gatgttgttc ctgtaactga gacaattggc
    28561 tgtatcggtg ctgaaggtga agaagtaaca gaagcatctt cttctgaaaa tacatcagtt
    28621 gaagaaaacg cgacacaagt aacttcagaa ccagaaaaag ttgaagaaac atcagaacca
    28681 agtgtgccag cagctacaag tggtgaaaaa gtacgtgcca ctcctgcagc tcgtaaatta
    28741 gcaagagaga tgtctattga ccttgcacta gtatctggaa caggggcaaa tggtcgcgtt
    28801 cacagagaag atgttgaaag cttcaagggt gctcaaccac gtattacacc attggcacgt
    28861 cgaattgctg aagatcaagg agttgatata gcagaaatca ctggcagtgg tattcgtggt
    28921 aaaattgtta aaaatgatgt tttggctgct atgtcaccgc aagcagcaga ggctcctgta
    28981 aaaacgaaag ctacaccaac aacagaagaa aaacaattac cagaaggtgt tgaagtcatt
    29041 aagatgtctg caatgcgtaa ggctatttct aaaggtatga ctaactctta cctcactgct
    29101 ccaagcttta cacttaacta tgatattgat atgactgaaa tgatggctct tcgcaagaaa
    29161 ttaattgatc caatcatggc taaaacaggc ttgaaagtca gctttactga tttgattgga
    29221 atggccgttg ttaagacatt gatgaaacca gagcatcgat acctaaatgc ttcattaatt
    29281 aacgatgcac aagaaattga gcttcataat tttgttaata ttggaatcgc agttggtctt
    29341 gatgatggtt tgattgttcc tgttgttcac aatgctgatc aaatgtcact ttctgacttt
    29401 gttatagcat ctaaagatgt tattaagaaa actcaagaag gtaaattaaa atcagctgaa
    29461 atgtcaggtt caacattctc aattactaac ttaggtatgt ttggtactaa gacgtttaac
    29521 ccaattatca atcaaccgaa ctcagcaatc cttggagtag gtgctactat tccgactcca
    29581 acagtggttg atggtgaaat tgtagctcga ccaatcatgg caatgtgttt aacaattgac
    29641 caccgtattg ttgatggaat gaatggtgct aagtttatgg ttgatcttaa gaacttaatg
    29701 gaaaacccat ttggtttact tatttgattc cgtaggtctt aatttgatat aataagttat
    29761 tcgaaaaacg aaagaggaaa taaaaaatgg cttttgatgt aattatgcca aaacttgggg
    29821 ttgacatgca agaaggcgaa atccttgagt ggaaaaaaaa tgaaggtgac accgttaatg
    29881 aaggtgatgt ccttcttgaa atcatgtctg ataaaaccaa tatggaaatt gaagcagaag
    29941 acactggtgt acttttaaaa attgtacatc aggcaggaga tgttgttcct gtcactgaag
    30001 tgattgccta tatcggagaa gaaggtgaag aagtaggtac gtcatcacct tcggctgatg
    30061 caactatcac agctgaagat ggtcaatcag tttctggacc tgctgctcct tcacaagaga
    30121 cagttgcagc agcaactcct aaagaagaac ttgcagctga tgagtacgat atcgtagtcg
    30181 taggtggtgg ccctgcaggt tattacgcag caatccgcgg tgctcaactt ggaggaaaaa
    30241 ttgccattgt tgagaaaact gaatttggcg gtacgtgttt gaatgttggt tgtatcccta
    30301 caaaaacata tcttaaaaat gctgaaatcc tagatggttt gaaagtagca gctggacgtg
    30361 gtatcaacct tgcttcaact aactatgcaa ttgatatgga taagactgtt gcctttaaaa
    30421 attctgtagt taagacattg actggtggtg tacgtggtct cttgaaagcg aacaaagttg
    30481 aaattttcaa tggacttgga caagtaaacc ctgataaatc tgttgttatt ggcgataaag
    30541 ttatcaaagg tcgtaacgtc gtccttgcga ctggttctaa ggtatctcgt attaatattc
    30601 caggtattga atcaccatta gtattaacat cagatgatat tcttgattta cgtgaaattc
    30661 ctaagtcact tgctgttatg ggtggtggtg tcgtaggtat tgagcttggt cttgtatggg
    30721 cttcatatgg tgttgacgta actgttattg aaatggctga tcgcatcatt ccagcaatgg
    30781 ataaagaagt atcgctagaa cttcaaaaaa tccttgctaa gaagggaatg aagattaaaa
    30841 catcagttgg tgtttctgaa attgttgaag ccaataatca attaacgctt aaattaaaca
    30901 acggcgaaga agttgttgct gataaagctc ttctttcaat tggacgtgtt ccacaaatga
    30961 atggtcttga aaatcttgag cctgaacttg agatggaacg tggacgtatt aaagttaatg
    31021 cttatcaaga aacttcaatt ccaggtatct atgcaccagg tgatgttaat ggaactcgta
    31081 tgttagctca tgctgcatac cgtatgggtg aagtagctgc tgaaaacgca ctcggcggta
    31141 ataagcgtaa agcacatcta gatttcactc cagcagctgt ttatactcat cctgaagtag
    31201 caatggtggg gatgacagaa gagcaagcac gtgaacaata tggtgacata ttagttggta
    31261 aaaatagctt tacaggcaat ggtcgtgcta tcgcttcaaa cgaagcacat ggttttgtaa
    31321 aagtaattgc tgaacctaag tataaagaga ttcttggtgt tcacattatt ggtccagctg
    31381 cagcggaatt gattaatgaa gcttctacga ttatggaaaa tgagttgact gtctatgatg
    31441 tagcacaatc tatccatggt cacccaacat tctctgaagt tatgtacgaa gctttcttag
    31501 atgttctcgg tgaagctatt cacaaccctc caaaacgcaa ataattttgt ttaatatatt
    31561 tgagattgag cttttatagc ttcaatctct tttcacgtat gtattgtctg aatattataa
    31621 aatattagtt aggaatttaa aatgaaatat atcgttaaca cgtctaatga tccggcttac
    31681 aatgttgctt tagaagctta cgcctttcaa aagttgacag atatcgatga aatctttatt
    31741 ctttggatta atgaaccggc tatcattatt ggacgccatc aaaatacaat tcaagaaatt
    31801 aacaaagagt tcattgataa aaatggcatc catgttgtcc gtcgtttgtc aggaggcgga
    31861 gcagtttatc atgacttgaa taatttaaac tacactatta tttctaataa tacacaagaa
    31921 ggtgcttttg atttccaaac tttttcaaaa ccagttattg atactcttgc taaattaggt
    31981 gttaaagcag aatttactgg tcgtaatgat ttagaaatca atggacaaaa atttgcagga
    32041 aatgctcaag cttattacaa aggtcgtatg atgcatcatg gttgtctttt atttgatgtt
    32101 gatatgtcag tcttagggca agcactcaaa gtatctaaag acaaaattga atctaaaggg
    32161 ataaaatctg tacgcgcacg tgttacaaat attgttgacc atctttcaga taaaattaca
    32221 gtacaagaat ttagcgatgc cattttagct caagtgaagg aagaatatcc agagatggat
    32281 gaatatgttt tgagtgatgc cgagttatca gaaatccaag ctatgcgcga taatcaattt
    32341 gctacatggg attggacgta tggtaaagca cctgaataca ccattgaacg tggtgtccgc
    32401 tatccagctg gaaaaattac gacttatgct aatgttgaaa attcaacgat aaaatctgtc
    32461 aaaatctttg gtgacttctt tggagtgaaa cctgtagatg atattgaaaa aatgttggaa
    32521 ggtgtccgct acgattacaa agatgtttta gcagcgctta aaacagttga tacaagtcaa
    32581 tacttctcac gtatgacacc cgaagagatt acaaaagcta ttgtagacta aaatcaaaaa
    32641 acctagtcaa agtctttgcc tttggctagg ttttttgtat ggttaagtgg ccttatagta
    32701 cgatgggcat gtcgtacatc tgaggtgcaa gtcctctgtg ggcacccgct accggtgaac
    32761 ccaatagcga ctcccaagct tgactatcgt gaggtagcgg ggagaggaag ggatagcgaa
    32821 atc
//
LOCUS       DBJYSH010000007        30926 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.007, whole genome shotgun sequence.
ACCESSION   DBJYSH010000007 DBJYSH010000000
VERSION     DBJYSH010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 30926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30926
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.007"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            376..1737
                     /locus_tag="ACUBV3_000270"
     CDS             376..1737
                     /locus_tag="ACUBV3_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000440330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3846806.1"
                     /translation="MERLKSSGLSMGKPVDYMGVNGIPIYTKTLSIDNKFAFENNSKD
                     SSYSSNINISEDKIKENDQKILDLIVKSGANNQNLTDEEKVIAFTKYIGEITNYDNEA
                     YRARNVDTEYYRASDLFSVTERKLAMCVGYSVTAARAFNIMGIPSYVVSGKSPQGISH
                     AAVRAYYNRSWHIIDITASTYWKNGNYKTTYSDFIKEYCIDGYDVYDPAKTNNRFKVK
                     YMESNEAFENWIHNNGSKSMLFINESAALKDKKPKDDFVPVTEKEKNELIDKYKKLLS
                     QIPENTQNPGEKNIRDYLKNEYEEILKKDNLFEHEHAEFKESLNLNESFYLQLKKEEM
                     KPSDNLKKEEKPRENSVKERETPAENNDFVSVTEKNNLIDKYKELLSKIPENTQNPGE
                     KNIRNYLEKEYEELLQKDKLFKHEYTEFTKSLNLNETFYSQLKEGEMKLSENPEKGET
                     NTN"
     gene            complement(1908..2522)
                     /locus_tag="ACUBV3_000271"
     CDS             complement(1908..2522)
                     /locus_tag="ACUBV3_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HHU3846807.1"
                     /translation="MSFLEELKNRRSIYALGRNTEVSDEKIVEIIKEAVRQSPSAFNS
                     QTSRVVILLNDEVTKFWDELVANDLVETMKVQGAPETAIAGTKEKLASFGASKGTVLF
                     FEDQDVVKSLQEQFVLYADNFPVWSEQSTGIASVNTWTALSAELGLGGNLQHYNPVID
                     ASVQAVYGVPASWKLRGQLNFGSIEAETGEKEFMNDDDRFKVIG"
     gene            complement(2655..3080)
                     /locus_tag="ACUBV3_000272"
     CDS             complement(2655..3080)
                     /locus_tag="ACUBV3_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HHU3846808.1"
                     /translation="MGNFKNSAVKSMVVMRKAFRTIDGKVSESFKEFELTPTQFAVLD
                     VLYAKGTMKIGELIENMLATSGNMTVVIKNMEKKGWVLRHSCPNDKRAFLVSLTTEGE
                     EVIKKALPEHIKRVEDAFSVLTETEQEDLINLLKKFKTL"
     gene            complement(3207..7613)
                     /locus_tag="ACUBV3_000273"
     CDS             complement(3207..7613)
                     /locus_tag="ACUBV3_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HHU3846809.1"
                     /translation="MSELFKKLMDQIEMPLEIKNSSVFSSADIIEVKVHSLSRLWEFH
                     FSFPELLPIEVYRELQTRLVNSFEKADIKATFDIRAETIDFSDDLLQDYYQQAFCEPL
                     CNSASFKSSFSQLKVHYNGSQMIISAPQFVNNNHFRQNHLPRLEQQFSLFGFGKLAID
                     MVSDEQMTQDLKSSFETNREQLLEKANQEAMQALEAQKSLEDSAPPSEEVTPTQNYDF
                     KERIKQRQAGFEKAEITPMIEVTTEENRIVFEGMVFSVERKTTRTGRHIINFKMTDYT
                     SSFAMQKWAKDDEELKKYDMISKGSWLRVRGNIENNNFTKSLTMNVQDIKEIVHHERK
                     DLMPADQKRVEFHAHTNMSTMDALPTVESLIDTAAKWGHPAIAITDHANVQSFPHGYH
                     RAKKAGIKAIFGLEANIVEDKVPISYNEVDMNLHEATYVVFDVETTGLSAANNDLIQI
                     AASKMFKGNIIEQFDEFIDPGHPLSAFTTELTGITDNHVRGSKPILQVLQEFQNFCQG
                     TVLVAHNATFDVGFMNANYERHNLPLITQPVIDTLEFARNLYPEYKRHGLGPLTKRFQ
                     VALEHHHMANYDAEATGRLLFIFLKEARENRDVTNLMELNTKLVAEDSYKKARIKHAT
                     IYVQNQVGLKNIFKLVSLSNVKYFEGVARIPRSVLDAHREGLLLGTACSDGEVFDALL
                     SNGIDAAVTLAKYYDFIEVMPPAIYRPLVVRDLIKDEVGIQQIIRDLIEVGRRLDKPV
                     LATGNVHYIEPEDEIYREIIVRSLGQGAMINRTIGRGEDAQPAPLPKAHFRTTNEMLD
                     EFAFLGKDLAYEIVVTNTNTFADRFEDVEVVKGDLYTPFVDRAEERVAELTYAKAFEI
                     YGNPLPDIIDLRIEKELASILGNGFAVIYLASQMLVQRSNERGYLVGSRGSVGSSFVA
                     TMIGITEVNPMPPHYVCPNCQHSEFITDGSCGSGYDLPNKNCPKCGTLYKKDGQDIPF
                     ETFLGFDGDKVPDIDLNFSGDDQPSAHLDVRDIFGEEYAFRAGTVGTVAEKTAFGFVK
                     GYERDYNKFYNDAEVERLATGAAGVKRSTGQHPGGIVVIPNYMDVYDFTPVQYPADDM
                     TAAWQTTHFNFHDIDENVLKLDILGHDDPTMIRKLQDLSGIDPSNILPDDPDVMKLFS
                     GTEVLGVTEEQIGTPTGMLGIPEFGTNFVRGMVNETHPTTFAELLQLSGLSHGTDVWL
                     GNAQDLIKEGIATLSTVIGCRDDIMVYLMHAGLQPKMAFTIMERVRKGLWLKISEDER
                     NGYIQAMRDNNVPDWYIESCGKIKYMFPKAHAAAYVLMALRVAYFKVHYPIFYYCAYF
                     SIRAKAFELRTMSAGLDAVKARMKDITEKRQRNEATNVENDLFTTLELVNEMLERGFK
                     FGKLDLYRSHATDFIIEEDTLIPPFVAMEGLGENVAKQIVRAREDGEFLSKTELRKRG
                     GVSSTLVEKFDEMGILGNLPEDNQLSLFDDFF"
     gene            complement(7736..8320)
                     /locus_tag="ACUBV3_000274"
     CDS             complement(7736..8320)
                     /locus_tag="ACUBV3_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HHU3846810.1"
                     /translation="MKSRKKDKLVLRLTTTLLVFGLGGVWFYNYKNDNVEPTVTSASD
                     QTTTFIQTISPTAIEISKTYDLYASVLLAQAILESSSGQSDLSKAPNYNLFGIKGEYK
                     GKSVQMPTLEDDGKGNMTQIQAPFRAYPNYSASLYDYAELVSSQKYASVWKSNTSSYK
                     DATAALTGLYATDTAYASKLNQIIETYSLDAYDK"
     gene            complement(8445..10298)
                     /locus_tag="ACUBV3_000275"
     CDS             complement(8445..10298)
                     /locus_tag="ACUBV3_000275"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HHU3846811.1"
                     /translation="MKQSKMLIPTLREMPSDAQVISHALMVRAGYVRQVSAGIYAYLP
                     LANRTIEKFKTIMRQEFEKIGAVEMLAPALLTADLWRESGRYETYGEDLYKLKNRDQS
                     DFILGPTHEETFTTLVRDAVKSYKQLPLNLYQIQSKYRDEKRPRNGLLRTREFIMKDG
                     YSFHKDYEDLDVTYEDYRKAYEAIFTRAGLDFKGIIGDGGAMGGKDSQEFMAVTPNRT
                     DLNRWLVLDKTIPSIDDIPEDVLEEIKVELSAWLVSGEDTIAYSTESSYAANLEMATN
                     EYKPSTKAATFEEVTKVETPNCKSIDEVAGFLSIDENQTIKTLLFIADEQPVVALLVG
                     NDQVNDVKLKNYLAADFLEPASEEQAKEIFGAGFGSLGPVNLPDSVKIIADRKVQDLA
                     NAVSGANQDGYHFTGVNPERDFTAEYVDIREVKEGEISPDGKGTLKFARGIEIGHIFK
                     LGTRYSDSMGANILDENGRSNPIVMGCYGIGVSRILSAVIEQHARLFVNKTPKGAYRF
                     AWGINFPEELAPFDVHLITVNVKDQESQDLTEKIEADLMLKGYEVLTDDRNERVGSKF
                     SDSDLIGLPIRVTVGKKASEGIVEVKIKASGDTIEVHADNLIETLEILTKK"
     gene            complement(10390..11649)
                     /gene="rseP"
                     /locus_tag="ACUBV3_000276"
     CDS             complement(10390..11649)
                     /gene="rseP"
                     /locus_tag="ACUBV3_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HHU3846812.1"
                     /translation="MLGILTFIIIFGVIVVVHEFGHFYFAKKSGILVREFAIGMGPKI
                     FSHIDKEGTTYTIRILPLGGYVRMAGWGDDKTEIKTGTPASLTLNKEGIVTRINLSGK
                     QLDNTSLPINVTAYDLEDKLTITGLVLSETKTYSVDHDATIIEEDGTEIRIAPLDMQY
                     QNASVWGRLITNFAGPMNNFILGLVVFIALAFIQGGVQDLSTNQVRVSENGPAASAGL
                     KNNDRILQIGSHKVSNWEQLTAAVEKSTRHLEKKQKLALKIKSKEVVKTINVKPQKVD
                     KSYIIGIMPALKTSFKDKLLGGLKLAWESFFRILNELKKLIAHFSINKLGGPVALYQA
                     SSQAAKNGFVTVLNLMGLISINLGIMNLIPIPALDGGKIVMNILEAIRRKPLKQETET
                     YITLAGVAVMLVLMIAVTWNDIMRAFF"
     gene            complement(11680..12474)
                     /locus_tag="ACUBV3_000277"
     CDS             complement(11680..12474)
                     /locus_tag="ACUBV3_000277"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002275264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HHU3846813.1"
                     /translation="MKERVIWGAVALAIFIPFLVMGGLPFQFLVGLLAMIGVSELLRM
                     RRLEIFSFEGALAMIGAFVLTVPLDSYLSFLPVDASLSAYGIVIFMILAGTVLNSNSY
                     SFEDAAFPIASSFYVGIGFQNLVSARMAGIDKVLLALFIVWATDIGAYMIGRQFGQRK
                     LLPSVSPNKTIEGSLGGIASAIVVAFFFMLFDKTVYAPHSFLVMLVLVAIFSIFGQFG
                     DLVESSIKRHFGVKDSGKLIPGHGGILDRFDSMIFVFPIMHFFGLF"
     gene            complement(12489..13241)
                     /locus_tag="ACUBV3_000278"
     CDS             complement(12489..13241)
                     /locus_tag="ACUBV3_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HHU3846814.1"
                     /translation="MFIFKRKKQVEMPLEKIPAHIGIIMDGNGRWAKKRLKPRVMGHK
                     AGMDALQEVTIAASGLGVKVLTVYAFSTENWSRPDDEVKFIMNLPVEFFDKYVPELDK
                     NNVRVQVIGDTHKLPKATYDAMQRACLRTKHNSGLVLNFALNYGGRSEITNAIKEIAQ
                     DVLEAKLNPDDITEDLVANHLMTNSLPYLYRDPDLIIRTSGELRLSNFLPWQSAYSEF
                     YFTPVLWPDFKKDELHKAIVDYNQRHRRFGSV"
     gene            complement(13408..13749)
                     /gene="yajC"
                     /locus_tag="ACUBV3_000279"
     CDS             complement(13408..13749)
                     /gene="yajC"
                     /locus_tag="ACUBV3_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000056605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HHU3846815.1"
                     /translation="MSLPIIMLVVMVGMMFFMQRQQKKQAQERQKQLNAVQKGDEIVT
                     IGGLFGVVDEVNTEAQRMVLDVDGVYLTFELAAIKSVVSKAATPTEPVEDVKIIEVNE
                     DENKETAIETE"
     gene            complement(13826..14170)
                     /locus_tag="ACUBV3_000280"
     CDS             complement(13826..14170)
                     /locus_tag="ACUBV3_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HHU3846816.1"
                     /translation="MYSFEELLATMTLITAAEIEDKIDSNQDFVLFIGRISCPFCHLF
                     VPKIVEVADEDEFELFHLDSEDFDHWTANKEFRNKYDIPTVPGLMVVKNGTIKVKCDS
                     KMTKEEIREFIG"
     gene            complement(14361..15524)
                     /locus_tag="ACUBV3_000281"
     CDS             complement(14361..15524)
                     /locus_tag="ACUBV3_000281"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0004473 - malate dehydrogenase
                     (decarboxylating) (NADP+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent malic enzyme"
                     /protein_id="HHU3846817.1"
                     /translation="MSENLGQLAINQARENGGKLEVISKVKVEDKRDLSIAYTPGVAS
                     VSSAIAEDVELAYELTTKKNTVAVVSDGSAVLGLGDIGPEAAMPVMEGKAALFKRFAN
                     VDAVPIVLKTNDTEEIISIVKAISPTFGGINLEDISAPRCFEIEQRLIEECDIPVFHD
                     DQHGTAIVVLAALFNSLKLVKKDIEDIRVVVNGGGSAGLSITRKLLSAGAKHVTVVDR
                     FGIINDKDRESLAPHHKAIAKLTNREFQSGSLEDALENADVFIGVSAPEALHAEWISK
                     MADKPIVFAMANPIPEIYPDQALKAGAYIVGTGRSDFPNQINNVLAFPGIFRGALDAR
                     AKTITVEMQIAAARGIASLIPEEELSTTHIIPNAFQNDVADVVAKSVSNAVQK"
     gene            complement(15549..16868)
                     /locus_tag="ACUBV3_000282"
     CDS             complement(15549..16868)
                     /locus_tag="ACUBV3_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003108040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HHU3846818.1"
                     /translation="MNNEDSRGQKQDLKAKLFHIKIGSVPLPVYVCLALLILLAGFLQ
                     KLPVNMLGGFAVILTMGWFLGTIGASIPGFKNFGGPAILSLLVPSILVFFNLINKNVL
                     ESTNMLMKQANFLYFYIACLVSGSILGMNRKMLIQGLLRMIFPMLLGMVCAMMVGTFV
                     GVILGLEWRHTLFYIVTPVLAGGIGEGILPLSLGYSSITGVASEQLVAQLIPATIIGN
                     FFAILCTALLNRLGEKKPHLSGQGQLVRLNKGEDMSDIIADHSGPIDVKKMGGGVLTA
                     CSLFIFGHLLQQLTGFPGPVLMIVAAAILKYINVIPRETQNGAKQLYKFISGNFTFPL
                     MAGLGLLYIPLKDVVATLSIQYFIVVISVVFTVISVGFFVSRFLNMNPVEAGIISACQ
                     SGMGGTGDVAILSTADRMNLMPFAQVATRLGGAITVITMTAILRMLF"
     gene            17040..18566
                     /locus_tag="ACUBV3_000283"
     CDS             17040..18566
                     /locus_tag="ACUBV3_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HHU3846819.1"
                     /translation="MKKKLSLWAFLSLILVTMTICIFSIFYYVTIHQSYRMVRVQEEK
                     ILKNTGYALSRNPQVIQTLKDNHYDQSLQKQMLFLSKKSNLDYIVLINLKGIRFTHPD
                     STKIGKPFQGGDEQAVFKGKAIMSTAEGSLGKSLRYLIPVYDHQKQVGAIAVGLKLTT
                     LGDLSQSSIKEFSKPLLISILISLVVTSIISYGLKKQLHNLHPSDIFQHLEERNATLD
                     QIQAAVFVIDQRHIIKRNNPAASLLFKKEGQRDLFSGKLLESLIPQLKQDHFSKKTEQ
                     VLHFQGQDYLLSISPITVKTQNRGYVVFLRNVTETLFTLDQLAHTTAYASALQAQTHQ
                     FMNQLHVIYGLADIEYYDELKIYLKELLEPQNEFLARLSMLVREPRLASFIIGEREKF
                     AEKHINLSTEILVEIPTKSTVEDVNNYLLLHRYINTKILTLLNSTTLVSLRLNYQNNL
                     IETDYQWENEKWLLNDYHQYFNDAYFQQLLVDSRATYKISLKQNKVIIRTNIIYSEGP
                     "
     gene            18568..19248
                     /locus_tag="ACUBV3_000284"
     CDS             18568..19248
                     /locus_tag="ACUBV3_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HHU3846820.1"
                     /translation="MDVLIIEDDPMVEFIHRNYLEKLNYFQNIYSTASQTQAIAYLND
                     IKIQLVLLDIHIKEGNGLELLKLLRNQHQNTEVIVISAANEAHTVKEAFHLGIVDYLI
                     KPFTFERFESSIEKFLNHYHTFEADKIYQDNIDHFQKIDSGWLEGEVKLDEKGLSEIT
                     YQHILDAIQELEQPFTIQELAKCSQFSHVSVRKYIAYMEEKGLLTSQQIYTKVGRPYK
                     VYKLIKKT"
     gene            complement(19269..20264)
                     /gene="galE"
                     /locus_tag="ACUBV3_000285"
     CDS             complement(19269..20264)
                     /gene="galE"
                     /locus_tag="ACUBV3_000285"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000279623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HHU3846821.1"
                     /translation="MAVLILGGAGYIGSHMVDQLITQGKEKVIVVDNLVTGHRQAVHS
                     DAIFYEGDLSDKTFMRQVFRENPDVDAVIHFAAFSLVAESMENPLKYFDNNTAGMIKL
                     LEVMNECDIKNIVFSSTAATYGIPEQVPILETAPQNPINPYGESKLMMETIMKWADQA
                     YGIKFVALRYFNVAGDKPDGSIGEDHKPETHLLPIILQVAQGVRDKIMIFGDDYNTPD
                     GTNVRDYVHPFDLADAHILAVDYLRQGNESNVFNLGSSTGFSNLQMLEAARRITGKEI
                     PAQKAARRPGDPDTLIASSEKARQILGWEPKFDNIDKIISSAWAWHSSHPNGYED"
     gene            complement(20352..21959)
                     /locus_tag="ACUBV3_000286"
     CDS             complement(20352..21959)
                     /locus_tag="ACUBV3_000286"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA];
                     GO_function: GO:0004574 - oligo-1,6-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009311 - oligosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HHU3846822.1"
                     /translation="MKKHWWHKATIYQIYPRSFMDSDGDGVGDIKGITSKLDYLEKLG
                     ITAIWLSPVYQSPMDDNGYDISDYQAIADIFGDMNDMDQLLQEANQRGIKIIMDLVVN
                     HTSDEHAWFVEARENPNSPERDFYIWRDEPNDLTSIFSGSAWEYDKVSGQYYLHLFSK
                     RQPDLNWENEALRHKIYDMMNFWIDKGIGGFRMDVIDLIGKIPDKGITGNGPKLHDYL
                     KEMNRASFGKHDLLTVGETWGATPDIAKQYSNPDNEELSMVFQFEHVGLQHKPDAPKW
                     DYSDGLDVPALKDIFTKWQTQLELGQGWNSLFWNNHDLPRVLSIWGNDSDNRKQSAKA
                     LAILLHLMRGTPYIYQGEEIGMTNYPFECLADVDDIESLNYAKEAMDNGVSEATILDS
                     IRKVGRDNARTPMQWSQEHQAGFTKGTPWLAVNPNYQEINVEAALNDTESIFYTYQKL
                     VALRKEHDWLVDADFKLLETADKVFAYVRQTDKERYLIVANLSDQNQSFEFPEAVKET
                     IISNTEVQEVLSSNTLKPWDAFCIELF"
     gene            complement(22086..23219)
                     /locus_tag="ACUBV3_000287"
     CDS             complement(22086..23219)
                     /locus_tag="ACUBV3_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HHU3846823.1"
                     /translation="MVELNLNHIYKKYPSASHYSVEDFDLDIKDKEFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELKIDGEVVNDKSPKDRDIAMVFQNYALYPHMTVYDNMAFGL
                     KLRKFSKQEIDKRVREAAANIGLTEFLERKPADLSGGQRQRVAMGRAIVRDAKVFLMD
                     EPLSNLDAKLRVSMRAEIAKIHQRIGSTTIYVTHDQTEAMTLADRIVIMSATKNPDGD
                     GTIGKIEQVGSPQELYNLPANKFVAGFIGSPSMNFFKVKVENGMIISEDGLRIAIPEG
                     QEKLLESRGYKGKELIFGIRPEDISSNLLVQDTYPNANVEAEVLVSELLGSETMLYVK
                     LGQTEFASRVEARDFHNPGEKVNLTFNVAKGHFFDADTEQAIR"
     gene            complement(23320..24171)
                     /locus_tag="ACUBV3_000288"
     CDS             complement(23320..24171)
                     /locus_tag="ACUBV3_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HHU3846824.1"
                     /translation="MDFKELFPEVITKQEVKQSEDYIIVEQDGHVLHFPKSSLTKREL
                     YLLQMTPSLEDASSVDSQNPWYRYLVEGRGRLPQSHSAVQFIFIEHQFTLSEELKDFL
                     SPLVINVETIMTINQTQSVMILNQDNFFNATELLTDILPTIENDFNTRLRCYFGNSWT
                     HLQAVDWKELYEEEYKLFTLFLSHKAEQHYCRFPKMALWALANQSPMPSIKAKCLQHI
                     LDTSDTSAIIKALWQEQGNLAKTAKALFIHRNSLQYKLDKFTQSSGLNLKILDDLAYA
                     YLISMDT"
     gene            complement(24266..25162)
                     /locus_tag="ACUBV3_000289"
     CDS             complement(24266..25162)
                     /locus_tag="ACUBV3_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HHU3846825.1"
                     /translation="MAITIQNHELQVTLKALGATMTSITDSQGVEYLWQGDATYWGGQ
                     APILFPICGSVRNDCVIYRPAQAPHFTGIIPRHGFVRHKTFDYDYISDSSVRFTIKSS
                     KEMLINYPYRFSLEITYTLRNKSIAITYIVKNLESEKNMPYAIGAHPGFNCPLFEKEV
                     FSDYYLEFEQFETCTIPESFPDTGLLDLQARHPFLENQKQLSLNHALFEKDAITLDQL
                     RSKTVYLKSRNHAKGIQLDFDDFENLILWTSNNGGPFLALEPWSSLSTSIEESDILED
                     KQNIVRLNPKQSKQHSIRITIL"
     gene            complement(25216..26193)
                     /gene="lacD"
                     /locus_tag="ACUBV3_000290"
     CDS             complement(25216..26193)
                     /gene="lacD"
                     /locus_tag="ACUBV3_000290"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000524687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HHU3846826.1"
                     /translation="MGLTEQKQKHMEQLSDKNGIISALAFDQRGALKRLMAKYQSEEP
                     TVSQIEALKVLVAEELTPYASSMLLDPEYGLPATKVLDDNAGLLLAYEKTGYDTSSTK
                     RLPDCLDIWSAKRIKEEGADAVKFLLYYDVDSSDEVNEEKEAYIERIGSECVAEDIPF
                     FLEILSYDEKITDSSGIEYAKIKPRKVIEAMKVFSNPRFNIDVLKVEVPVNMDYVEGF
                     AQGETAYNKATAAAYFREQDQATLLPYIFLSAGVPAQLFQETLVFAKEAGAKFNGVLC
                     GRATWAGSVKEYVEKGEAGARQWLRTIGFQNIDELNKILQKTATSWKER"
     gene            complement(26195..27127)
                     /locus_tag="ACUBV3_000291"
     CDS             complement(26195..27127)
                     /locus_tag="ACUBV3_000291"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009024 - tagatose-6-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HHU3846827.1"
                     /translation="MILTVTLNPSIDISYCLENFNMDTVNRVTDVSKTPGGKGLNVTR
                     VLSQLGDNVVATGLLGGDFGDFIRSGLDALEIRHQFLSIGGETRHCIAVLHEGQQTEI
                     LEKGPHITKDEADAFLNHLKLIFDAATIITVSGSLPKGLPSDYYARLISLANHFNKKV
                     VLDCSGEALRSVLKSSAKPTVIKPNLEELTQLIGKPISYSLDELKSTLQQDLFRGIDW
                     VIVSLGARGAFAKHGNHYYQVTIPKIEVINPVGSGDATVAGIASALEHQLDDTNLLKR
                     ANVLGMLNAQETLTGHINLTYYQELISQIQVKEV"
     gene            complement(27138..27653)
                     /gene="lacB"
                     /locus_tag="ACUBV3_000292"
     CDS             complement(27138..27653)
                     /gene="lacB"
                     /locus_tag="ACUBV3_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HHU3846828.1"
                     /translation="MKIAVGCDHIVTYDKIAVVDYLKTKGYEVIDCGTYDNIRTHYPI
                     YGKKVGEAVASGKADLGVCICGTGVGINNAVNKVPGIRSALVRDLTSAIYAKEELNAN
                     VIGFGGKITGGLLMTDIIEAFIRAKYKPTKENKVLIEKIAEVETHNAHQEENDFFTEF
                     LDKWNRGEYHD"
     gene            complement(27674..28099)
                     /gene="lacA"
                     /locus_tag="ACUBV3_000293"
     CDS             complement(27674..28099)
                     /gene="lacA"
                     /locus_tag="ACUBV3_000293"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HHU3846829.1"
                     /translation="MTIIIGADAHGVELKEVIRQHLTSLGKEIIDLTDTSKDFVDNTL
                     AIVAKVNQKEDNLGIMVDAYGVGPFMVATKVKGMIAAEVSDERSAYMTRAHNNARMIT
                     LGSEIVGPGVAKHIVEGFVDGTYDAGRHQIRVDMLNKMC"
     gene            complement(28347..30797)
                     /locus_tag="ACUBV3_000294"
     CDS             complement(28347..30797)
                     /locus_tag="ACUBV3_000294"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004308 - exo-alpha-sialidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HHU3846830.1"
                     /translation="MTLTFGKPSITIRRLGVITSTYMMLGFILLCGFLHPSTIYSEEI
                     KSDINSFISIDDEKTTVPIFDSTLDKLTNSIEERQLELDSDTSDSLIVDDQKDKDMVI
                     VLGDSDSQEKLSPPLKSEIATEENRKQANLAELNATQPNNRTTYIIPESSHSIAEQQR
                     FLIESKGYSVALLNSDEFRKTAREDRGFERDKLRSLDIIPKGDLSTSNVIGNTDIASQ
                     ISLGFKKNAIQEHHLTKTFSQKDGKLSSVIEGMLAIGKEKVEKEIKYSGNLWQKLKAK
                     AHCLVCCVDNLNFEDIKSYFQYYCHLNHQLKLPKGAILSAKTEVYRGGDFGRKNKDNV
                     FGYRIPSLLKTEKGTLLVGADERIEQACDWGNIGMVIRRSEDDGVTWGKRETIVNLRN
                     NPRVPLVTSGDYSGSPINMDMALVQDTSSKTKRIFSIYDMFPEGRGVISIANTPEKEY
                     TQIGGQSYLNLYNNGKKSKVFTIRDKGIVYNFKGKKTDYHVITETTKSDHSNLGDIYK
                     GKQLLGNIYFTKHKTSPFRLAKSSYVWMSYSDDDGRTWSSPRDITASLRQKGMKFLGI
                     GPGKGIVLKWGPHAGRIIIPAYSTNWKSHLRGSQSSRLIYSDDHGKTWHTGKAVNDNR
                     ILSNGEKIHSLTMDNKKEQNTESVPVQLKNGDIKLFMRNLTGNLEVATSKDGGETWQN
                     HVKRYKEVHDAYVQLSAIRFEHDKKEYILLVNANGPGKKRQDGYARLAQVNRNGSFKW
                     LYHHHIQDGSFAYNSVQQLNNDKFGVLYEHREKHQNSFTLNYKVFNWSFLSQNTEKQG
                     TLWEKMAANWHVLFKFYL"
BASE COUNT        10008 a         6181 c         4823 g         9914 t
ORIGIN      
        1 aatctagttg aaaaaaacag tgaagataag tttattcaag agttaaatag gtataaaaca
       61 gagataccaa atttcaaagg ttttaatgtt tggattttag gtgataaagg ctattacaaa
      121 aaccttatca atttagaaga aattaaaaat atccaagcta cattaaaaaa agaaagaaat
      181 gaagaatatg tctttgtcaa attaaatggg aaaatcgcac atgatacaac cgtattcttg
      241 atgaataaaa aacataaatt acttaaaaat atagaggaat ttaagacgat aacccaaaaa
      301 aggctaactg agagaggaaa gttcccatat gataccgttc attctacgtt tgaaattaag
      361 gatgaaaact ttatcatgga aagattgaaa tcatcagggt tatctatggg taaaccagtt
      421 gattatatgg gcgtaaatgg tatccctatt tacactaaaa cattatctat agataataag
      481 tttgcctttg aaaataattc aaaagattct tcatattctt caaacattaa tattagtgaa
      541 gataaaataa aagaaaatga ccaaaaaatt ctagatttaa tagttaaatc tggagctaat
      601 aatcaaaact taacagatga agaaaaagta attgctttca ctaaatatat aggtgaaata
      661 actaattatg acaatgaagc ttacagagct agaaatgtcg acacagaata ttacagagct
      721 tcagatttat tttctgtaac agaaagaaaa cttgctatgt gcgttggcta tagtgtaaca
      781 gccgctaggg catttaatat tatggggata cctagttatg tcgtttctgg taaaagtcct
      841 caaggaatat ctcatgcagc tgtgagagcg tattataata gaagttggca tatcatagat
      901 atcacagcca gtacatattg gaaaaatggt aattacaaaa caacgtattc cgattttatt
      961 aaagaatact gtatagatgg ttatgacgtt tatgatcccg caaaaacaaa taatagattt
     1021 aaagttaagt atatggaatc aaatgaagcg tttgaaaatt ggatacataa taatggaagc
     1081 aaatctatgt tatttattaa tgaatctgca gctctaaaag ataaaaaacc aaaagatgat
     1141 tttgtaccag taacagaaaa agaaaaaaac gagctgatag ataagtataa aaaattattg
     1201 tcacagattc ctgaaaacac acaaaatcca ggagaaaaaa acataagaga ttatttgaaa
     1261 aatgaatatg aagaaatatt aaagaaagat aatttatttg agcatgaaca tgcagaattt
     1321 aaagaaagtc taaatttaaa tgaatcattc tacctacaat taaagaaaga agaaatgaaa
     1381 ccgagtgata atctcaaaaa agaagaaaaa ccaagagaaa attcagtaaa agagagggag
     1441 actccagctg aaaacaatga ttttgtttca gtaacagaaa aaaacaactt gattgataaa
     1501 tataaagaat tattatcaaa aatcccagag aatacacaaa atcctgggga aaagaatata
     1561 cgaaattatc tagagaaaga atatgaagaa ctactacaaa aagataaatt attcaagcat
     1621 gagtacacag agtttacaaa aagtctaaat ttgaatgaaa cattctattc acaattaaaa
     1681 gaaggagaaa tgaaactaag tgaaaatccc gaaaaaggag aaacgaacac gaattaacct
     1741 ttcaaatatc tacatcaaat aacagaagtc tctgtatcaa gcgaattgta gcttttttga
     1801 atatactaaa aacactccta cctctatttt actagaagta ggagtgtttt ttcattttag
     1861 ttcatagatg cagtgtcatt gctatccttg cctttttttg tacagtatta tccgatgact
     1921 ttaaagcggt catcatcatt catgaattct ttttctcctg tttcagcttc gattgaacca
     1981 aaatttagtt gtccacgaag tttccaacta gctggaacac cataaacagc ttgtactgat
     2041 gcatcaataa ctggattgta atgttgtaaa ttaccaccaa gtccaagttc tgcagaaagg
     2101 gctgtccaag tatttactga ggcaatacct gttgattgct cagaccaaac tggaaagtta
     2161 tccgcataaa gaacgaattg ttcttgaaga gatttgacaa catcttgatc ttcaaagaag
     2221 agtactgttc cctttgaagc tccaaaacta gctaattttt ccttagttcc agcaatagct
     2281 gtctctggag caccttgtac tttcattgtt tctaccaaat cattagcaac caattcatcc
     2341 cagaacttag taacttcatc attcaataaa atgacaacac gggaagtttg actattaaaa
     2401 gcagatgggg attggcgaac tgcctcttta ataatctcta ctattttctc atcagaaact
     2461 tctgtattgc gaccaagagc gtaaatagag cgacggtttt ttaattcttc caaaaatgac
     2521 atattttttc tccttgttct tcaatctcag atatcttact acatttgttc tagtgacata
     2581 tgaattagat taaagttatt tattactatg tagtaattat attaccacta tatcgtaaag
     2641 tcaaagaatg tgactcacaa ggttttaaat ttttttaaaa ggttaatcaa gtcttcttgc
     2701 tctgtttctg ttaagactga aaaagcatct tctacacgtt taatatgctc tggtagagct
     2761 ttttttatca cttcttctcc ttcagttgtt aaactcacca aaaaggctct cttatcattc
     2821 ggacaagaat ggcgtaatac ccatcccttt ttttccatat ttttaataac aacagtcata
     2881 ttacctgatg tagcaagcat attttcaatc aattctccaa ttttcattgt gcctttagca
     2941 tagagaacat ccaaaactgc aaactgagtt ggtgttaatt caaattcttt aaatgattct
     3001 gagacttttc cgtctattgt tctaaatgct tttcgcatga caaccataga tttaaccgct
     3061 gaatttttaa agtttcccat ttcatctccc aaacgtattt tttcttattt tatcactaat
     3121 tagttatttc aagcaagaat atcatctttg ccctatcaaa agcagctggc aaataataag
     3181 gttgagtaac tactcaacct tattacttag aagaaatcat caaaaagact caactgattg
     3241 tcttctggta aatttcccaa aatacccatc tcatcaaatt tttcaactaa ggtacttgag
     3301 actcctcctc gtttacgaag ctctgttttt gataaaaatt caccatcttc acgtgcccga
     3361 actatctgtt ttgcaacgtt ttctcccaag ccttccatag caacaaatgg agggattagc
     3421 gtatcctctt caataataaa gtcagtcgca tggctacgat aaagatctaa tttcccaaat
     3481 ttaaagccac gctcaagcat ttcattaacc aactctaatg tcgtaaaaag atcattttcg
     3541 acattcgtcg cctcattacg ctgacgcttc tcagtgatat ccttcatgcg cgccttaacg
     3601 gcatcaagtc cagcactcat tgtgcgtaat tcaaaagctt tggcgcgaat agaaaaataa
     3661 gcacaataat aaaatattgg ataatgtact ttaaagtacg ccacacgtaa tgccattaaa
     3721 acataggctg ctgcatgggc tttagggaac atatacttga tttttccaca cgattcaata
     3781 taccaatccg ggacattatt atcacgcatt gcttggatat aaccattacg ctcatcttct
     3841 gaaattttca accataaccc cttacgcacg cgctccatga ttgtaaaggc catctttggt
     3901 tgtaggccag cgtgcattaa ataaaccatg atgtcatcac gacaaccaat aacagttgat
     3961 aaggtagcaa taccttcctt gattaagtct tgagcattac ctaaccaaac atccgtacca
     4021 tgtgatagcc ctgacagttg caacaattct gcaaatgtgg ttggatgggt ttcgttcacc
     4081 ataccacgaa caaagttcgt accaaactct ggaataccaa gcattcctgt aggagttcca
     4141 atttgctctt ctgtcactcc taaaacttcc gtaccagaaa ataacttcat aacgtctgga
     4201 tcatctggaa ggatatttga tggatcaatt cctgataaat cctgaagttt acgaatcata
     4261 gtaggatcat catgtcctag aatatctaac ttcaagacat tctcatcaat atcatggaaa
     4321 ttgaagtgag ttgtctgcca agcagctgtc atatcatctg ctggatattg aaccggagta
     4381 aaatcataaa catccatata gttaggaata acaacaatac caccggggtg ttgacctgta
     4441 gaacgtttaa ctcccgcagc accagtcgct aaccgttcga cctctgcatc gttataaaat
     4501 ttattgtaat ctcgttcgta acctttgaca aaaccaaagg cagttttttc agctacggta
     4561 cctaccgttc ctgcacgaaa agcatattct tctccaaaga tatcacgaac gtctaagtga
     4621 gcagaaggtt gatcatctcc agagaaattc agatcaatat caggaacctt atctccatca
     4681 aatcctaaga aagtttcaaa cggaatatcc tgtccatctt tcttgtacaa agtaccacat
     4741 ttaggacaat ttttatttgg taaatcatag cctgaaccac aagaaccatc tgtgataaat
     4801 tcagaatgct gacaatttgg gcaaacataa tgaggtggca ttggattaac ttcagtaatt
     4861 cctatcatgg tcgctacaaa actcgaccca acagacccac gtgatcctac tagataacca
     4921 cgttcattag aacgttgcac caacatttgc gaagcaagat agataacggc aaatccatta
     4981 ccaagtatag atgctaactc tttttcgata cgtaaatcaa taatatcagg aagaggatta
     5041 ccatatattt cgaatgcttt agcataagtt aattcagcaa ctcgttcttc agcacgatca
     5101 acaaacggag tatagagatc cccttttaca acttcaacgt cttcaaatcg atctgcaaaa
     5161 gtattcgtat tggttacaac gatctcatag gctaaatctt taccaagaaa ggcaaattca
     5221 tctaacatct catttgttgt gcggaaatgt gcttttggca aaggcgctgg ctgagcatcc
     5281 tccccacgac ctatagtcct attaatcatg gcaccttgtc caagactacg aacaataatc
     5341 tcacggtaaa tctcatcttc aggctcaata taatgaacat ttcccgtagc taaaacaggc
     5401 ttatctaaac gccgaccgac ctctattagg tctctaataa tttgttgaat ccccacctca
     5461 tctttaatta aatcacgtac taccaagggg cgataaatag caggtggcat gacttcaata
     5521 aaatcataat atttagctag ggtcactgct gcatcaattc catttgataa caaggcatca
     5581 aaaacttctc catcagaaca ggcagtacct agcaagagac cttctctgtg agcatctaaa
     5641 actgacctag gaattcttgc caccccttca aaatatttaa catttgaaag gctaactaac
     5701 ttaaatatat ttttaagtcc aacctgattt tggacataaa tggttgcatg ctttatgcgt
     5761 gcctttttgt aagagtcttc agctaccaat ttagtattga gctccatcaa attagtcaca
     5821 tcacgatttt cccgcgcctc ttttaagaaa ataaagagta agcgacccgt tgcttcagca
     5881 tcgtagttgg ccatatgatg atgttccaaa gcaacttgga aacgtttggt tagaggcccc
     5941 aaaccatgac gcttatattc aggataaaga tttcttgcaa attctaaggt atcaatgacc
     6001 ggctgtgtga tcaaaggcag attatgacgt tcataattcg cattcataaa accaacgtca
     6061 aaggttgcat tatgcgcaac taaaaccgta ccttgacaaa aattctgaaa ttcctgaagg
     6121 acttgtaaaa taggttttga acctctgaca tgattatccg taatacctgt caattcagtt
     6181 gtaaaggcag aaagaggatg acctgggtca ataaactcat caaattgctc aataatatta
     6241 cccttgaaca tttttgatgc tgcaatctga attaggtcat tattagctgc agaaagacct
     6301 gtagtttcca catcaaatac aacataagtt gcttcgtgca aattcatatc aacttcgttg
     6361 taggaaatag gcactttatc ttcaacaata tttgcctcta atccaaaaat tgcttttata
     6421 cctgcttttt ttgctctgtg gtagccatgc ggaaaacttt gaacattggc atggtcagta
     6481 attgcaatag ctgggtgtcc ccacttagct gcagtatcaa tgaggctctc gacagttggt
     6541 aaagcatcca tcgttgacat attggtatga gcatgaaatt ccacacgttt ttgatctgct
     6601 ggcattaagt ctttacgttc atgatgcaca atctctttga tatcttgaac attcattgtc
     6661 aaagatttag taaagttatt gttctcaata ttccctctga ctcgcaacca cgaaccttta
     6721 gaaatcatat cgtacttctt caattcttca tcatctttag cccacttttg catagcaaaa
     6781 cttgaggtat aatcagtcat cttaaaatta atgatatgac gtccagtgcg ggttgttttt
     6841 cgttcaacac taaaaaccat tccttcaaaa acgatacgat tttcttctgt tgtcacctca
     6901 atcatgggtg taatctctgc tttttcaaag ccagcttgac gttgtttgat acgctctttg
     6961 aaatcataat tttgagttgg ggtcacttct tctgatggag gcgctgaatc ttctagtgat
     7021 ttttgtgctt ccaaggcttg catagcttct tggttagctt tttcaagcaa ttgctcgcga
     7081 ttagtttcaa atgatgattt taagtcttgc gtcatttgtt catcagaaac catatcaata
     7141 gcaagtttac caaatccaaa caatgaaaac tgttgttcca aacgcggcaa atgattctga
     7201 cgaaaatgat tattattgac aaattgaggt gctgaaataa tcatctgact gccattgtaa
     7261 tgaactttta attgtgaaaa agaactctta aaacttgcac tattacacaa gggttcacaa
     7321 aaagcttgtt gataataatc ctgtagcaga tcatcagaaa aatcaatagt ttctgctctt
     7381 atatcaaaag tagcctttat atctgctttt tcaaaactat tcactaaacg agtttgtaat
     7441 tcacgataca cctcaattgg caataattct ggaaatgaaa aatgaaattc ccataagcgt
     7501 gatagagaat gaacctttac ctcgataata tcagcagacg aaaaaacact tgaattctta
     7561 atttcaagcg gcatttcaat ctgatccata agtttcttga acaattctga catagttttc
     7621 tcctgaatat cttacttcca ttttatcaca tatctgtaaa tatttcgaga taacaaaagg
     7681 aggctgttaa aaaaggaatt tattagcctt cttaacaaca ctctcttaac tgaaactatt
     7741 tatcataagc atctagactg taggtttcaa taatttggtt taatttacta gcataagcag
     7801 tatctgtcgc ataaagacct gttagagctg cagtagcatc cttataagaa gaggtatttg
     7861 atttccaaac agatgcatac ttttgactag atactaactc agcataatca tatagtgaag
     7921 cagaataatt tggataggcg cgaaaaggag cttggatttg agtcatattg cctttcccat
     7981 catcttctaa agtaggcatt tggacagatt tacctttata ttctcctttg atgccaaaga
     8041 ggttataatt aggagcctta gacaaatctg attgtccact ggatgattcc aaaatagctt
     8101 gtgctaataa gactgacgca tacaaatcat aggtcttaga aatttcaata gctgttggag
     8161 aaatcgtttg aataaaagtc gtcgtttgat ccgatgcact agtgactgtc ggttcgacat
     8221 tatcattttt ataattataa aaccaaaccc cacccaaacc aaaaaccaat agtgttgttg
     8281 ttaacctcaa taccaattta tctttttttc gacttttcat gatggctcct ttctaaaata
     8341 tcatttcatt ataacaaaag gcagttgctt tttaccaata agaaccatac taaatttcac
     8401 tccatataaa aaagttagca catcagacta acttttttat cggcttattt ttttgttaga
     8461 atctctaaag tttcaattaa gttatcagcg tgaacctcaa tcgtatcacc acttgcttta
     8521 attttcactt caacgatacc ttcagaagct ttcttcccaa cagttactcg aattggaagt
     8581 ccaattaagt cactgtctga gaatttagaa ccaacacgtt cattacgatc gtctgtcagg
     8641 acttcataac ctttaagcat caaatcagct tcaattttct cagtaaggtc ttgtgactct
     8701 tgatctttga cattgacagt aatcaaatgt acatcaaatg gggctaattc ttctgggaaa
     8761 ttaatccccc aagcaaagcg ataagctcct tttggtgtct tattcacaaa aagtcgggca
     8821 tgttgttcaa tgacagctga gagaatacga ctaacaccaa taccataaca ccccataaca
     8881 ataggatttg agcggccatt ttcatcaagg atgttcgctc ccatactatc tgaataacgg
     8941 gtacctagtt taaagatatg tccaatttca ataccacgag caaatttcag agtaccttta
     9001 ccgtctggag aaatctcccc ttcttttact tcacgaatat caacgtattc agctgtgaaa
     9061 tcacgttctg gattaactcc agtaaaatga taaccatctt ggttagcacc agacacagca
     9121 ttagcaaggt cttgaacttt acgatcagca ataattttaa cagagtcagg taggttaact
     9181 ggcccaagag aaccaaaacc tgcaccaaaa atctcttttg cttgctcttc acttgcaggc
     9241 tctaagaaat cagccgctaa ataatttttt aacttcacat cattgacttg atcgttacca
     9301 actagtaaag caaccacggg ctgttcatca gcaataaaca atagtgtctt gatagtttga
     9361 ttttcatcaa ttgataaaaa tccagctacc tcatcaattg atttgcagtt aggagtttct
     9421 acttttgtta cttcttcaaa ggtagcagcc ttagtagatg gtttgtactc attcgttgcc
     9481 atctcaaggt tagcagcata gcttgattca gtagagtatg caatagtgtc ctcaccagaa
     9541 actagccaag ccgagagttc taccttaatt tcttctaaaa catcctctgg aatatcatca
     9601 atagaaggaa tagttttatc aaggactaac caacgattta aatctgtacg attgggagtt
     9661 actgccataa attcttggct atctttacct cccatagcac ctccgtcacc tataatgcct
     9721 ttaaagtcaa gtccagcacg tgtgaagatg gcttcatatg ctttacggta atcttcgtaa
     9781 gtgacatcaa ggtcttcata atctttgtgg aagctgtatc catctttcat gataaattca
     9841 cgtgttcgaa gaagtccatt acgaggacgc ttctcatcac gatatttaga ctgaatttga
     9901 taaaggttta gtggtaattg cttataagat tttactgcat cacgaacaag agttgtgaaa
     9961 gtctcttcat gagttggtcc taagataaaa tccgattgat cacgattctt taatttataa
    10021 aggtcttcac catatgtttc gtagcgccca gattcacgcc ataaatctgc tgtaagaaga
    10081 gctggagcta acatttcaac tgcaccaatc ttctcaaatt cttgacgcat aattgtttta
    10141 aatttttcaa tagtacggtt agctagaggc aaataagcat agattccagc cgaaacttgg
    10201 cggacatatc ctgcacgaac cataagagca tggctgataa cctgagcatc acttggcatt
    10261 tcgcgaagtg ttggaataag cattttactt tgtttcatta caataagata cgactaatca
    10321 aagttgattc tttttcaatt gagaattgca attgaaaaat tcgtatttcc tttcacgttt
    10381 tattatcttt tagaagaaag ctcgcatgat atcgttccag gtcacagcaa tcattaatac
    10441 taacataact gctaccccag cgagcgtaat ataagtttct gtttcttgtt taagtggttt
    10501 gcgacgaatg gcttctaaaa tattcatcac tatcttaccc ccgtcaaggg ctggtatagg
    10561 gatgagattc ataataccta agttgataga gattaatccc attaagttga gaactgtgac
    10621 gaaaccattt ttagcagctt ggctactagc ctgatagaga gctactggac ctcccaattt
    10681 attaatacta aaatgagcaa taagtttctt taattcattt aaaattctaa agaaactttc
    10741 ccatgctagt tttaatccac ctaaaagttt gtctttaaag cttgtcttta aagctggcat
    10801 aatgccgatg atgtaggatt tatcaacttt ctgtggctta acgttgatcg ttttcaccac
    10861 ctctttgctc ttaatcttaa gggcaagttt ttgttttttt tctaaatgcc tagttgactt
    10921 ttctactgct gctgtcaact gttcccagtt agaaactttg tgcgacccta tttgtaaaat
    10981 acgatcatta ttttttaaac ctgcactagc ggcaggacca ttttctgata cacggacctg
    11041 attggttgat aagtcttgaa cacctccttg aataaaagca agtgcaataa agactacaag
    11101 acctaaaata aaattattca tcggacctgc aaaattggta attaagcgcc cccatacaga
    11161 agcattctga tactgcatat ctaagggagc aatacgaatt tcagtaccat cctcctcaat
    11221 gatagtagca tcgtgatcaa cactataggt ttttgtctca cttaagacca atcctgtgat
    11281 agttagttta tcttctaaat cataagctgt tacattgatt ggtaaagaag tattgtctaa
    11341 ttgttttcct gataaattaa tacgagttac aatgccttct ttatttaaag taaggctagc
    11401 cggcgtccct gtcttaattt cagtcttatc atcaccccaa ccagccatac ggacataacc
    11461 acctagcggt aatatacgaa tagtataggt tgttccttct ttatcaatgt gagaaaagat
    11521 ttttggaccc ataccaatag caaattcacg aacaagaatt cctgatttct ttgcaaaata
    11581 gaaatgccca aattcgtgaa caactacaat gacaccaaaa ataataataa aggttaaaat
    11641 tcctagcatt caattcctct ctaaatacaa tatattattt tagaataagc caaagaagtg
    11701 catgattggg aaaacaaaaa tcatactatc aaagcgatcc aaaatccccc catgacccgg
    11761 aatcaattta cccgaatctt taacaccaaa atgacgttta atactacttt caactaaatc
    11821 tccaaattga ccaaaaatgc taaagatagc cactaaaact aacatgacta ggaagctatg
    11881 aggcgcatag acggtcttat caaaaagcat aaaaaagaaa gctaccacaa tggctgaagc
    11941 aatccctcct agactccctt caattgtttt atttggagac actgagggta gtagtttgcg
    12001 ttgtccaaat tgacgaccaa tcatataagc accgatatca gtcgcccaga caataaataa
    12061 agctaacaac accttatcta ttccagccat acgagcactg actagatttt gaaagccaat
    12121 acccacataa aaactagacg caattggaaa ggcagcatct tcaaaactgt aagaattact
    12181 gttcaaaaca gtacctgcca aaatcataaa aataacaata ccgtatgctg ataaactagc
    12241 gtcaactggt aaaaaggaca aataactatc taaaggaact gttaaaacaa aggctcctat
    12301 catagctaat gcaccttcaa aggaaaatat ttctaaacga cgcatacgaa gtaattcaga
    12361 aacaccaatc atagctaaaa gtccaactaa aaattggaaa ggcaaaccac ccattactaa
    12421 aaaagggata aatatggcaa gcgcgacagc tccccaaata acacgttctt tcattttctc
    12481 tcttctttct atacactacc aaatcgacga tggcgttgat tataatccac aatggcctta
    12541 tgtagttcgt cttttttaaa atctggccat aaaacgggag taaagtaaaa ttcactataa
    12601 gctgattgcc aaggtaaaaa gttactcaag cgtaactctc cactagttcg gataatcaaa
    12661 tcagggtctc tataaagata gggtaagcta tttgtcatta aatgatttgc tactaaatcc
    12721 tctgtaatat cgtcaggatt caacttagct tccaaaacat cctgagcaat ttccttaatg
    12781 gcattagtga tttctgaacg tccgccataa tttagcgcaa aattcaagac gagccccgaa
    12841 ttatgttttg tcctcaaaca agcgcgttgc attgcatcgt atgttgcctt aggtaattta
    12901 tgagtatctc cgatgacctg tacacgtaca ttatttttat ctaactctgg tacatattta
    12961 tcaaaaaatt caaccggaag gttcataata aacttaactt catcatctgg acgtgaccaa
    13021 ttttctgtag aaaaggcata gacagttagt actttaacac ctaatcctga agctgctatt
    13081 gtaacttctt gaagagcatc catccccgcc ttatgtccca taacgcgtgg ttttaaacgt
    13141 ttttttgccc aacgtccgtt accatccatg ataataccaa tatgtgctgg tattttctcc
    13201 aaaggcattt ctacttgttt ttttctttta aatataaaca taatgttcct taatttagca
    13261 atcgtttttc ttatcagccc ttttaaaaga gctggaaata tcccatacta taacatttta
    13321 ccacatttca acttattttt gagtattaaa atgccatttg tgacagcaaa ctaaaaagac
    13381 cagaacaatc atctggtcct acatatctta ttcagtttcg atggctgttt ctttattttc
    13441 gtcttcattg acttcaataa tcttaacatc ttcaactggt tctgttggtg ttgctgcctt
    13501 agagacaaca gatttgatag ctgccaactc aaacgtcaaa tagacaccat caacatctaa
    13561 aaccattcgt tgtgcttcag tattaacttc atcaactact ccaaaaagac caccaattgt
    13621 gacaatttca tcaccttttt gaacagcatt caattgtttt tgacgttctt gtgcttgttt
    13681 cttttgctga cgttgcatga agaacatcat accaaccata acaacaagca taataatagg
    13741 taagctcatt tgattctcct ttccgtgttc tacgtaatca ttactatagc agatttatgc
    13801 tatatctgca aggagatact gtctcttagc caataaattc acggatttct tcttttgtca
    13861 tttttgaatc gcacttcacc ttgatagttc cgtttttaac aaccatcaaa cctggcactg
    13921 taggaatgtc atatttattt cgaaattctt tatttgcagt ccaatgatca aaatcttcac
    13981 tatctaaatg gaataattca aattcatctt catcagctac ttctacaatt ttaggaacaa
    14041 agaggtgaca aaatggacaa gaaatacgac caatgaacag tacaaaatct tgatttgaat
    14101 caattttatc ttcaatttcg gcagccgtta tcaaagtcat ggttgccaaa agttcttcaa
    14161 aagaatacat agttaaccaa ctttctaaat cttatattta aagaataacc tatgatggca
    14221 aaacttgcaa ggcaagagta acaacgatcc tatcaaaatc attaactcac ttctcgcttc
    14281 aaactatcct caaaaatgct cactcatatt taaatgactc atcaaaaaaa ctgagcatag
    14341 ctcagttttt attttacacc ttatttttgt acagcattac tgacgctttt agcaactaca
    14401 tcggccacat cattttgaaa agcatttgga atgatgtgag ttgtgcttaa ttcttcctcc
    14461 ggtattaaac tagctatgcc tctcgctgcc gcaatttgca tttcaacagt tatcgttttt
    14521 gctctagcat ctaatgctcc tctgaaaata cccggaaatg ctaaaacatt attaatttga
    14581 tttggaaaat cactacgacc agttccaact atgtaagctc cggcttttaa ggcttgatct
    14641 ggataaattt ctggaatagg attcgccatt gcaaatacaa taggtttatc agccatcttg
    14701 gaaatccatt ctgcatgtaa tgcttccgga gcagaaacac ctataaaaac atcggcattc
    14761 tccaaagcat cttctaagga tccagattgg aattctctat tagtcaattt tgcaatcgct
    14821 ttgtgatgag gggctaatga ctcccgatct ttatcgttaa taatgccaaa tcgatcaact
    14881 accgttacat gttttgctcc tgctgataga agcttccttg ttatagataa gcccgctgaa
    14941 cctccaccat taacaactac tctgatgtct tcaatgtctt ttttaactag cttcaaactg
    15001 ttaaacaaag ccgctaatac tacgattgct gtaccatgtt ggtcatcatg gaaaactggt
    15061 atatcacatt cttcaattag gcgttgttca atttcgaaac aacgtggtgc tgaaatatcc
    15121 tctaaattaa tccctccaaa agtcggcgaa atagctttta ctatagaaat aatttcctct
    15181 gtatcatttg ttttcaacac aattggcaca gcatctacat ttgcaaaacg tttaaagagt
    15241 gctgctttac cttccatgac tggcattgct gcctcaggac caatatctcc taatccaaga
    15301 acagctgaac catcactcac tacggctact gtattttttt tagtcgtcaa ctcatatgca
    15361 agctccacat cctctgcaat agctgatgat actgacgcca cccctggggt ataggcaata
    15421 ctaagatccc tcttatcttc caccttaact tttgaaatta cttctaattt tccaccattt
    15481 tctctagctt ggttgatagc taattgacct aaattttcac tcattttgat aactcctttt
    15541 gataaatatt agaataacat gcgtaaaatg gctgtcattg tgataacagt aatagctcct
    15601 cctaaacgcg tagcaacttg agcaaatggc atcaagttca ttcggtctgc tgtacttaaa
    15661 atggcaacat ctcctgttcc tcccatacca ctttgacaag ctgaaataat acctgcttca
    15721 acaggattca tattaaggaa tcgcgataca aagaatccaa cagaaataac tgtaaataca
    15781 acactaataa caactatgaa atattgtatg ctaagcgttg ccacaacatc ttttaacggg
    15841 atataaagca atcctagacc tgccattaga ggaaatgtaa aattaccaga aataaattta
    15901 taaagttgct tagctccatt ttgtgtttct ctaggaataa cattaatata tttcaaaata
    15961 gctgctgcaa caatcattaa tacgggacca ggaaatccag ttaattgctg caacaaatgt
    16021 ccaaaaataa agagactaca tgctgttaaa acacctccac ccattttctt aacgtcaatt
    16081 gggccagaat gatcagcaat aatatctgac atgtcctctc ctttatttaa ccttactaat
    16141 tgcccttgac cagacaagtg tggtttcttt tctcccaaac gattcaatag tgcagtacat
    16201 aaaatggcaa agaaattacc aataatagtg gctgggatga gttgagcaac tagttgttca
    16261 ctagctacac cggtaattga actatagcct aacgataatg gtaaaatacc ttcaccaata
    16321 ccaccagcta aaacgggtgt tacgatataa aacaaagtgt gtcgccactc taagcctaaa
    16381 ataacaccga caaatgtccc taccatcatc gcacaaacca ttcctaataa catggggaaa
    16441 atcattctta gcaatccctg aatcaacatt ttccgattca tccctaaaat actaccggac
    16501 actaaacaag caatataaaa ataaagaaag ttagcttgct tcatcaacat atttgttgat
    16561 tctaaaacat ttttattaat gaggttgaaa aacaccaaaa tagatggtac taataaagat
    16621 aaaatagctg ggccaccgaa gtttttaaaa ccaggaatgc tagctccgat agtccctaag
    16681 aaccacccca ttgttaagat aactgcaaat cctcctagca tattgactgg caatttttgt
    16741 aaaaagcctg ctagaagaat caataatgct aaacaaacat atactggtag gggaactgac
    16801 cctatcttaa tatgaaatag tttagccttt aagtcttgct tttgacctct agaatcctca
    16861 ttattcacta ccttcacgtc tgccatgaaa gcacctcctt ttacttctta tgtacagttt
    16921 actttgggac ttagtgataa ggaagtttat ttaagtaatt gaattatttc aacttattta
    16981 attaatttaa gctggattct agtttttagg ctatgttata ctaaactcgg agggtgctga
    17041 tgaaaaaaaa actatctctt tgggcatttc tatcattgat tttagtcact atgaccattt
    17101 gtatattttc tattttttat tatgtcacta tacatcagtc ctatcgtatg gtaagggtac
    17161 aggaagaaaa aatcttaaaa aatactggtt atgctttatc gagaaatcca caagttatac
    17221 aaacactcaa agataatcac tatgatcaaa gtctccaaaa acaaatgtta ttccttagca
    17281 aaaaatcaaa tttagactat attgtcctta taaatttaaa agggatccgt tttacccacc
    17341 ctgactccac taagattggt aaaccatttc aagggggtga tgaacaagct gtttttaagg
    17401 gaaaagctat tatgtctact gcagaaggtt ctttaggaaa atctttacga tacttaattc
    17461 cagtatatga tcaccaaaaa caggttgggg caattgcagt tggcttaaaa ttaacgacac
    17521 ttggagactt atctcaaagt agcataaaag agtttagtaa gcctctcttg attagtattt
    17581 tgattagctt agtcgtcaca agtataattt cttatggctt aaaaaaacaa cttcacaacc
    17641 tccatccaag tgatattttt caacatttag aagagcgcaa tgcaacactg gaccaaattc
    17701 aagccgctgt atttgttatt gaccagaggc atatcatcaa aagaaataat cctgcagcta
    17761 gtttattatt caaaaaagag gggcaacgag acctattttc aggtaaatta ttagaatctt
    17821 taattcctca actcaagcag gatcattttt caaaaaaaac tgaacaagta ttacattttc
    17881 aaggacaaga ctatttacta tctatctcgc caattacggt taaaacacaa aatagaggct
    17941 atgttgtctt cttaaggaat gttactgaaa cacttttcac tttggatcaa ttagcacaca
    18001 ctacagctta tgcttctgcg ctacaggccc aaacacatca atttatgaat caacttcatg
    18061 ttatttatgg acttgcagac attgaatact acgacgagtt gaaaatttat ttaaaagaat
    18121 tattggagcc acaaaatgaa tttttagcac gcttatcaat gttagttaga gagcctcgac
    18181 tggctagttt cattattggc gaaagagaaa aattcgcaga aaaacacatc aatctatcca
    18241 cagaaatttt agtagaaatc cctaccaagt caactgttga ggatgttaat aactatctct
    18301 tgctccacag atatattaat acgaagatac tcacgctatt aaattcaacg actttagttt
    18361 ctttacgttt aaattatcaa aataacctca ttgaaacaga ttatcaatgg gagaatgaga
    18421 agtggttact caatgactac catcagtatt tcaacgacgc ttattttcaa cagttacttg
    18481 tagattcaag agctacttat aagatctctt taaaacaaaa taaggttatt ataagaacaa
    18541 atattatcta ctcagaaggg ccttagtatg gacgtattaa ttatcgaaga tgatcctatg
    18601 gtggaattta tccaccgcaa ttaccttgaa aaactcaatt attttcaaaa catctattca
    18661 actgctagtc aaacacaagc tattgcttat cttaatgaca ttaaaatcca acttgttctt
    18721 ttagatattc atattaaaga aggcaatggt ctagaattat taaagttatt acgcaatcaa
    18781 caccaaaata ccgaagtcat tgttatttca gcagccaacg aggcgcacac tgtcaaagaa
    18841 gcatttcatt taggtattgt agattacctg ataaaaccat ttacatttga acgctttgag
    18901 tctagtattg aaaaattcct aaatcattat catacatttg aagctgataa aatctatcaa
    18961 gacaacatcg atcactttca aaaaatagat tctggatggc tagagggaga ggttaaactt
    19021 gatgagaaag ggctctctga aattacctat cagcatattc ttgatgccat tcaagagtta
    19081 gagcagcctt tcactatcca agagttagca aaatgcagtc aattttctca tgtctctgtt
    19141 aggaaatata ttgcctatat ggaagaaaaa ggccttctga caagtcagca aatttatact
    19201 aaggtcggtc gtccttacaa agtttataag cttataaaaa aaacttaggc ctgtagacct
    19261 aagttttttt aatcttcgta gccatttgga tgactagaat gccatgccca tgccgatgaa
    19321 ataattttat caatattatc aaatttaggc tcccacccaa ggatttgacg agctttctct
    19381 gaggaagcaa taagcgtatc tggatctcct ggacgacgag ctgccttttg agcaggaatt
    19441 tctttcccag taatacgacg agctgcctct aacatctgaa ggttagaaaa acctgtcgaa
    19501 gatccgagat taaacacgtt tgattcattc ccttggcgaa ggtaatcaac tgctaatata
    19561 tgagcatctg ccaaatcaaa tggatgcaca taatctcgaa cattagttcc atctggagta
    19621 ttgtaatcat ctccaaaaat cattattttg tcacgtactc cttgagcaac ttgaagaatg
    19681 attggtaaca aatgtgtttc tggtttatga tcttccccaa ttgagccatc cggtttatct
    19741 ccagcaacgt tgaagtaacg tagagcgaca aacttaatac cgtaggcttg atcagcccat
    19801 ttcataattg tttccatcat aagcttactt tcgccataag gattaatagg attttgagga
    19861 gctgtttcta aaataggaac ttgttctgga ataccatatg ttgcagctgt tgatgaaaag
    19921 acaatatttt tgatatcaca ttcattcatt acttctaaaa gttttatcat tccagcagtg
    19981 ttattatcaa aatattttaa aggattttcc attgactcag caactagtga aaatgccgca
    20041 aaatggataa cagcatctac atcaggattt tccctaaaaa cttgacgcat aaacgtttta
    20101 tcggataaat ctccttcata aaaaatcgca tctgaatgca ccgcttgacg atgacctgtt
    20161 accaagttat caacaacaat aactttctct tttccttgtg tgattaactg atcaaccata
    20221 tgcgagccaa tgtagccggc gccacctaaa atcagaactg ccatattttt ctcctatttc
    20281 aatactcttt taacatcatt tgttacctca ttatactgag aataagcttt ttttgcaaat
    20341 atctaccgaa attagaatag ctctatgcag aaggcatccc acggcttgag agtgttactt
    20401 gatagtactt cttgaacctc tgtatttgaa ataatagttt ctttaacagc ttctggaaat
    20461 tcaaaagatt ggttttgatc ggataagttc gcaactatta aataacgttc tttatctgtt
    20521 tgacgaacat aagcaaatac cttatctgct gtttccaaaa gtttaaaatc agcatcaact
    20581 aaccagtcgt gttctttacg taaagcaact aatttttgat aagtataaaa aatactttcc
    20641 gtatcattta acgctgcctc cacattaatt tcctgataat tgggattgac tgctagccat
    20701 ggggtacctt tagtaaaacc agcttgatgt tcttgactcc actgcattgg agtacgagca
    20761 ttatcacgac cgactttacg aatactatct aaaattgttg cttcagatac cccattatcc
    20821 atggcttctt tagcatagtt aagggattca atatcatcta catctgctaa acactcaaat
    20881 ggataattag tcataccaat ttcttcacct tgatagatat aaggcgttcc acgcatcaaa
    20941 tgtagtagga ttgctagagc cttggcagat tgttttctgt tatcactatc atttccccag
    21001 atcgagagca ctcgaggaag gtcatgatta ttccagaata aagaattcca accttggcca
    21061 agctccaatt gtgtttgcca ttttgtaaaa atatctttca atgctggaac atctaatccg
    21121 tcactgtaat cccacttagg agcgtcaggt ttatgttgaa gacctacatg ttcaaattga
    21181 aagaccatag ataattcttc attatcggga tttgagtatt gttttgcaat gtctggcgtt
    21241 gctccccaag tttcacccac cgtcaataag tcatgctttc caaaactagc tctgttcatt
    21301 tctttaagat aatcatgaag ttttggtcca ttaccagtaa tgcccttatc tggaattttc
    21361 ccaattaaat caatgacatc catacggaaa ccaccaatac ccttatcaat ccagaaattc
    21421 atcatatcat aaattttatg acgtaaagct tcattctccc agttgagatc tggctgacgt
    21481 ttgctaaaaa ggtgcaaata atattgacct gatactttat catattccca agctgagcca
    21541 ctaaaaatag aagtcaaatc gttaggttca tctcgccaaa tataaaaatc acgttcagga
    21601 ctattagggt tttctcgcgc ttctacgaac caagcatgtt catcagaggt atgattcact
    21661 accaaatcca tgatgatttt aatcccgcgt tgatttgcct cttgtaacag ttggtccata
    21721 tcattcatat caccaaaaat atctgcaatg gcttgataat ctgaaatatc ataaccattg
    21781 tcatccattg gactttgata aacaggtgat aaccagatag cagtaatccc taatttttca
    21841 aggtagtcta gtttgctagt aatcccttta atgtccccca caccatcacc atctgaatcc
    21901 ataaatgaac gtgggtaaat ttgataaatc gtagctttat gccaccagtg tttcttcatt
    21961 attcttctcc tatcaataat tcattcatct tttattttaa cgtttttcct ctttagtgca
    22021 agcgtttgcg taaaaacatt ataaaaaggc tggaacccaa cctttttatg aatatcgctt
    22081 ataccttaac gaatagcttg ttcagtatct gcatcaaaga aatgcccctt agcaacatta
    22141 aacgttaggt taactttttc tcctggatta tggaagtcac gcgcttcaac acgagaagca
    22201 aattctgttt gacctaactt aacataaagc atagtttcgc taccgagtaa ttcagaaact
    22261 aaaacttctg cttcaacatt ggcatttgga taagtatctt ggacaagaag attgcttgaa
    22321 atgtcttctg gacgaatacc aaaaatcaat tcttttcctt tatagcctcg tgattctaaa
    22381 agtttttctt gaccttcagg gatagcgatt cgaagaccat cttcactaat aatcatacca
    22441 ttttcaactt taactttgaa gaagttcata gatgggctac caatgaatcc cgcaacaaat
    22501 ttattggcag gaagattata gagttcttgt ggtgaaccaa cttgctcaat tttaccaatt
    22561 gtaccatcac catcagggtt cttagtagct gacataatga caatacggtc tgccaaagtc
    22621 atcgcttctg tttggtcgtg ggtaacataa atagttgttg aaccaatacg ttgatgaatt
    22681 tttgcaattt cagcacgcat tgaaacacgt aatttagcat ctaagttaga taaaggctca
    22741 tccattaaga acactttggc atcacgcaca atagcacgtc ccatagcaac acgttggcgt
    22801 tgtccacctg acaaatccgc aggtttgcgc tctaagaatt cagtaaggcc aatattagct
    22861 gctgcttcac gaacgcgctt atcaatttct tgttttgaga atttacgcaa tttgagccca
    22921 aaagccatat tatcgtaaac agtcatatgt ggataaagag catagttttg gaaaaccata
    22981 gcaatatcac gatctttagg tgatttgtca ttgacaactt cgccatcaat cttgagctca
    23041 ccttcactaa tatcttcaag acctgcgatc atacgaagtg ttgttgattt cccacaacct
    23101 gatggtccaa caaaaacaat aaattctttg tctttaatat ccaagtcaaa gtcttcaaca
    23161 gaataatgcg atgcacttgg atattttttg taaatatgat ttaaatttaa ttctaccatt
    23221 agattgacct cttttattat tgtgatttta ttatagcaaa gaaagggttt tcatataatg
    23281 gtaaccttgc ctaaatttct tgtgcaaact aactaaattt taggtatcca tcgaaatcaa
    23341 ataggcatag gctaaatcat ctaatatttt taaattaagt cccgaagatt gggtaaattt
    23401 atctaattta tattgcaatg aattccgatg aatgaaaagt gccttagccg ttttagcaag
    23461 atttccttgc tcttgccaca gggcttttat tatagccgaa gtatcactcg tatcaaggat
    23521 atgttgtaaa cattttgctt taatagaagg catgggcgat tgatttgcca aagcccataa
    23581 agccatcttt ggaaatcgac agtaatgttg ttcggccttg tgactaagaa aaagcgtaaa
    23641 aagtttatat tcttcttcat ataattcttt ccagtccact gcttgtagat gcgtccaact
    23701 atttccaaaa tagcacctta aacgcgtgtt aaagtcattt tcaatcgttg gtaaaatatc
    23761 tgttagtaat tccgtagcat taaagaaatt atcctgattt aaaatcatga cactttgggt
    23821 ttgattaatt gtcataattg tttctacgtt tattactaaa ggcgacaaga aatccttaag
    23881 ttcttctgat aaggtaaatt gatgctcaat aaaaataaat tggactgccg aatgagattg
    23941 gggcaacctt ccacgacctt caactagata gcggtaccaa ggattctgag aatccactga
    24001 actcgcatct tccaggcttg gtgtcatttg taataagtat agttcacgct tagttagcga
    24061 agattttgga aagtggagga catgaccatc ttgctcaacg atgatataat cctcactctg
    24121 tttaacttcc tgtttagtaa taacttctgg aaacagttcc ttaaaatcca tggcatcctc
    24181 cactacagtt ttgttcatta tatcatattt ataacctctt tttgagcaca gaaaaagagg
    24241 agaatgtctc ctcagaaaat ttctgttata aaatagtgat ccgaatagaa tgctgtttcg
    24301 actgctttgg attaagccgt acgatatttt gtttatcttc taaaatatca gactcttcaa
    24361 tagaagttga caaagaactc cagggttcca aagccaaaaa aggaccacca ttgttacttg
    24421 tccaaagaat aagattctca aaatcatcaa agtcaagttg gattcctttt gcatgatttc
    24481 tagattttaa gtaaaccgtc ttagatcgta gttggtctaa agtaatagca tctttttcaa
    24541 ataaagcatg attaagagat aattgttttt gattttctaa aaaaggatgt ctagcttgta
    24601 aatctaacaa gcctgtatcc ggaaacgact caggaattgt gcaagtttca aactgttcaa
    24661 actctagata gtaatcacta aatacttcct tttcaaacaa agggcaatta aagcctgggt
    24721 gagctccgat agcatatggc atattttttt ctgattctaa gtttttgaca atatatgtaa
    24781 tggcaatgct tttatttctt aaggtgtaag ttatttctaa actaaaacga taaggataat
    24841 ttatcaacat ttctttacta cttttaattg taaaacgaac agaagaatcc gatatgtaat
    24901 catagtcgaa tgttttgtga cgtacaaagc catgccttgg tatgataccc gtaaaatgtg
    24961 gagcttgagc tggtctgtaa atgacgcaat catttcgaac acttccgcat ataggaaata
    25021 aaataggagc ttgcccaccc caataagtgg cgtccccttg ccaaaggtac tcaacgcctt
    25081 gactatctgt aatagaagtc attgttgcac ctaacgcttt taatgtcact tgcaactcat
    25141 gattttgtat cgtaatagcc attatttttc ctttcataaa gaacaataat tgctttacca
    25201 gcttttctct agcaattatc tttccttcca agaagttgct gttttttgca aaattttatt
    25261 tagttcatca atattttgaa aacctattgt acgtaaccat tgacgtgcac cagcttctcc
    25321 tttttcaaca tactctttaa cacttccagc ccaagttgct ctaccacaaa ggacaccgtt
    25381 aaatttagca ccagcttctt tagcaaacac aagagtctct tgaaaaagct gagcaggaac
    25441 tcctgcactc aaaaaaatgt aggggagtag tgtcgcctga tcttgttctc taaaatatgc
    25501 cgcagctgtc gctttattat aagcagtctc tccttgcgca aaaccttcta catagtccat
    25561 attgacaggc acttccacct tcaagacatc aatattaaat cgaggatttg aaaaaacttt
    25621 catagcttca ataaccttac gtggcttaat tttggcatac tcaatacctg agctatccgt
    25681 aatcttttca tcatatgaca aaatttcaag gaaaaaagga atgtcttctg caacacattc
    25741 agaaccaatc cgttcaatat aagcttcttt ttcttcgttt acttcatccg aactatcaac
    25801 atcataataa agtaagaact taactgcgtc tgctccctct tctttaatgc gtttcgcaga
    25861 ccaaatgtct aaacagtctg ggagtcgctt ggtactactg gtatcataac ccgttttttc
    25921 gtacgcaagt aaaagaccgg cattgtcatc taaaactttc gtagctggca acccatattc
    25981 tggatctaac aacattgatg aagcgtaagg ggttaattct tcagctacca aaactttaag
    26041 tgcttctatt tgagaaacag tgggctcctc agattgatat tttgccatta atcgcttcaa
    26101 tgctccacgt tggtcaaaag ccaaggccga aataatacca tttttatcac tcaattgttc
    26161 catatgtttt tgtttttgtt ctgttaatcc catcctatac ctctttcact tggatttgac
    26221 tgattaattc ttgatagtag gtcagattga tatgccctgt taatgtttct tgtgcattaa
    26281 gcatacccaa gacattagct ctcttaagca aatttgtatc atctagttga tgttccaaag
    26341 cagacgcgat tcctgcaacc gttgcatccc ctgaaccaac tggattaata acttctattt
    26401 ttggaatcgt cacttgatag tagtgattcc catgtttagc aaaagctccc ctagcaccta
    26461 aagaaacaat aacccaatca attcctctaa aaagatcttg ctgtaaggta gattttaatt
    26521 cgtctaaact atagctaatt ggttttccaa ttaattgtgt taattcttca aggttaggtt
    26581 taatgacggt cggcttagca ctagatttta aaaccgaacg taatgcttct cctgaacagt
    26641 ctaaaacaac tttcttatta aagtgattcg ctagggagat taacctagca taataatctg
    26701 aggggagtcc ctttggtaaa cttcctgata ctgttatgat agttgcagca tcaaagataa
    26761 gttttagatg attcaaaaag gcgtctgctt catctttagt aatatgtgga cctttttcta
    26821 gaatttcagt ctgctgaccc tcatgtaaaa ctgcaataca atgacgtgtt tctccaccaa
    26881 tacttaaaaa ttggtgtctg atttccaatg catctaagcc acttcggatg aaatctccaa
    26941 aatccccccc taatagacca gttgccacta cattatcgcc aagttgagaa agaacacgag
    27001 tcacatttaa ccccttaccc ccaggtgttt tggagacgtc ggtaactcga tttacagtat
    27061 ccatgttaaa gttttctaag cagtacgaaa tatcaataga agggttcaaa gttactgtta
    27121 aaatcataga tatctcctta gtcgtgatat tcaccacgat tccatttatc taagaactct
    27181 gtgaaaaagt cattctcttc ttgatgggca ttatgagtct caacctcggc tatcttttca
    27241 attaatactt tgttttcttt tgttggttta tatttagctc ttataaacgc ctcgatgata
    27301 tctgtcatta acagcccacc agtaattttc ccaccaaatc cgataacgtt agcatttaat
    27361 tcttctttag catagatagc tgatgtcaaa tcacgtacca gagctgagcg aattcctgga
    27421 actttattaa cagcattatt aataccaact ccagttccac aaatacatac acctaagtcg
    27481 gcttttccac tagcgacagc ttctcctact ttcttaccat aaataggata gtgagtacgg
    27541 atattatcat aagttccaca atcaataacc tcataccctt ttgtttttaa ataatccaca
    27601 actgcaattt tgtcataagt tacaatatga tcacaaccga ccgcaatttt catctgcttt
    27661 acctctctta cgcttagcac attttattca acatatcgac ccgaatttga tgacgacctg
    27721 catcgtaagt cccatcaaca aaaccctcta ctatatgttt agcaactcct ggaccaacaa
    27781 tctctgaacc taaagtaatc atcctagcgt tattatgagc tctagtcata taagctgagc
    27841 gttcatctga aacttctgct gcaatcatcc ccttaacctt agtggctacc ataaaaggac
    27901 caacaccgta agcatcaacc attattccta agttatcttc tttttgattc actttagcaa
    27961 cgattgctaa ggtattatca acgaaatctt tgctagtatc cgttaaatca ataatttcct
    28021 tacctaaact tgtaaggtgt tgtctaatca cttccttaag ttcaacacca tgcgcatccg
    28081 ccccaataat aattgtcatt tctttctcct tatttcactt ttaatacaat tgtatgtagt
    28141 tggaagatat tgtttgtttt tgtttgcaat atcatcatat atcttatttt tgtttttgtc
    28201 aatgttttgt tttgtttttg tttgaaatga acaaaaaaaa ctccgtgata actatgtcaa
    28261 ctaaaattca ctactagcta gctatcacga agtttgatac ttcttattat ttttatttgt
    28321 aggaacgctt attccgcaag ccataatcat aaataaaatt taaacaaaac atgccaattt
    28381 gctgccattt tctcccataa agtgccttgc ttctctgtat tttgactaag aaaactccaa
    28441 ttaaaaactt tgtaatttaa agtaaaacta ttttgatgtt tttctctatg ttcataaagg
    28501 acaccaaatt tatcattatt aagttgttga acagagttgt aagcaaacga accatcttga
    28561 atgtgatggt gatataacca cttaaaacta ccatttcgat taacttgcgc tagacgtgca
    28621 tatccatctt gacgcttctt acctggccca ttagcattca ctaataaaat atactctttt
    28681 ttgtcatgct caaagcgaat agctgataat tggacgtaag catcatgaac ttccttatat
    28741 cgtttaacat ggttttgcca agtctccccg ccgtctttac ttgtggctac ttctaggtta
    28801 ccagttagat tcctcataaa taacttaatg tccccatttt tcaattgaac gggtacggat
    28861 tctgtatttt gttctttttt attatccatt gttaaggagt gaattttttc accattagaa
    28921 agtatacggt tatcattgac tgcttttcca gtatgccacg tttttccatg gtcgtctgaa
    28981 taaattaggc gtgaagattg tgaacctctt agatgagatt tccaattcgt agaataggca
    29041 ggaataataa tacgaccagc gtgtggcccc cattttaaaa ctataccttt tccaggtcct
    29101 attcctaaaa atttcatgcc tttctgacga agacttgctg ttatatctct aggtgatgac
    29161 catgtcctac catcatcatc gctatatgac atccacacat agcttgattt tgctaaacga
    29221 aatggtgacg ttttatgttt tgtaaaatat atatttccaa gtagctgttt tcccttataa
    29281 atatccccta gatttgaatg gtcactttta gtagtttctg ttataacatg ataatcagtc
    29341 tttttccctt taaaattata tacaatacct ttgtcacgga tagtaaaaac cttcgatttc
    29401 tttccattat tatataaatt aagataagac tgtcctccga tttgggtata ttctttttca
    29461 ggtgtgttag caatactaat aacgcctctt ccttctggaa acatatcata tattgaaaaa
    29521 atacgtttcg tcttagagct agtatcttga actaatgcca tatccatatt aataggtgag
    29581 ccactatagt caccactagt aactagcgga actctagggt tattacggag attgacaata
    29641 gtttctcttt ttccccaagt gacaccatca tcctcactac ggcgaataac cattcctatg
    29701 tttccccaat cacaagcttg ctcaattctt tcatcagctc ccacaagtaa agttcctttt
    29761 tccgttttta ataatgaggg gatacggtaa ccaaacacat tatctttatt ttttctccca
    29821 aaatctcctc ccctatatac ttctgtttta gcagaaagta tagcaccttt aggtaatttg
    29881 agctgatggt ttagatgaca ataatattga aaataagatt ttatatcttc aaaattcaaa
    29941 ttatcaacac agcaaacaag gcagtgtgcc ttagctttta atttttgcca taaattacca
    30001 ctatatttta tttccttctc tactttctct ttgccaatag caagcatccc ctctataaca
    30061 gacgataact ttccatcctt ttgagagaat gttttagtaa gatggtgttc ctgtatcgca
    30121 ttctttttaa agcccaacga tatctgacta gcaatgtccg tattacctat gacattactt
    30181 gtcgataaat ctcccttagg aatgatatcc aaagacctca acttatccct ttcaaaacct
    30241 ctatcctctc tcgctgtctt tctaaattca tcgctattaa gtaatgcaac cgaataaccc
    30301 tttgattcta tcaggaatct ctgttgttct gcaatggaat gactgctttc gggtataata
    30361 taggtagttc tattattagg ttgagttgca tttagttctg ctaaatttgc ctgtttacga
    30421 ttttcttctg tcgctatttc cgattttaga ggaggtgaca atttttcttg actgtcagaa
    30481 tcacctaaga ctattaccat atctttatct ttttgatcat ctactattaa actgtccgat
    30541 gtatcagaat ctaattctaa ctgcctctcc tctattgaat tagtaagctt atctaaagta
    30601 gagtcaaata tgggaacagt agttttttca tcatctattg atataaaaga gtttatatca
    30661 gattttattt cctcactata aattgtacta ggatgcaaaa atccacataa taagataaaa
    30721 cctagcatca tatatgtact agttatcact cctagacggc gtatcgttat agaaggtttc
    30781 ccaaaggtta atgtcataga aactccaagt cttaaaatat tggttgcata catcaactct
    30841 agaaaaatgg gagttcagta atgtttccaa aaaggattgt ataccaactt atcactattt
    30901 tcaatatatt tttataattt tactag
//
LOCUS       DBJYSH010000008        26913 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.008, whole genome shotgun sequence.
ACCESSION   DBJYSH010000008 DBJYSH010000000
VERSION     DBJYSH010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 26913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26913
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.008"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            complement(308..694)
                     /gene="rplQ"
                     /locus_tag="ACUBV3_000295"
     CDS             complement(308..694)
                     /gene="rplQ"
                     /locus_tag="ACUBV3_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HHU3846831.1"
                     /translation="MAYRKLGRTSSQRKAMLRDLTTDLLINESIVTTEARAKEIRKTV
                     EKMITLGKRGDLHARRQAAAYVRNEIASENYDEASDKYTSTTALQKLFDDIAPRYAER
                     NGGYTRILKTEPRRGDAAPMAIIELV"
     gene            complement(709..1647)
                     /locus_tag="ACUBV3_000296"
     CDS             complement(709..1647)
                     /locus_tag="ACUBV3_000296"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HHU3846832.1"
                     /translation="MIEFEKPIITKIDENKDYGRFVIEPLERGYGTTLGNSLRRVLLS
                     SLPGAAVTSIKIDGVLHEFDTIPGVREDVMQIILNVKGLAVKSYVEDEKIIELDVEGP
                     AEITAGDILTDSDIEIVNPDHYLFTIAEGHSLKATMTVAKNRGYVPAEGNKKDDAPVG
                     TLAVDSIYTPVKKVNYQVEPARVGSNDGFDKLTIEIMTNGTIIPEDALGLSARVLIEH
                     LNLFTDLTEVAKATEVMKETEKVNDEKVLDRTIEELDLSVRSYNCLKRAGINTVFDLT
                     EKTEPEMMKVRNLGRKSLEEVKIKLADLGLGLKNDK"
     gene            complement(1697..2080)
                     /gene="rpsK"
                     /locus_tag="ACUBV3_000297"
     CDS             complement(1697..2080)
                     /gene="rpsK"
                     /locus_tag="ACUBV3_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HHU3846833.1"
                     /translation="MAKPTRKRRVKKNIESGVAHIHATFNNTIVMITDVHGNALAWSS
                     AGALGFKGSRKSTPFAAQMAAEAAAKSAQEHGLKTVEVTVKGPGSGRESAIRALAAAG
                     LEVTAIRDVTPVPHNGARPPKRRRV"
     gene            complement(2098..2463)
                     /gene="rpsM"
                     /locus_tag="ACUBV3_000298"
     CDS             complement(2098..2463)
                     /gene="rpsM"
                     /locus_tag="ACUBV3_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HHU3846834.1"
                     /translation="MARIAGVDIPNDKRVVISLTYVYGIGLSTSKKILAAAGISEDIR
                     VKDLTPDQEDAIRREVDAIKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGKAVAIAGKKK"
     gene            complement(2481..2597)
                     /gene="rpmJ"
                     /locus_tag="ACUBV3_000299"
     CDS             complement(2481..2597)
                     /gene="rpmJ"
                     /locus_tag="ACUBV3_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HHU3846835.1"
                     /translation="MKVRPSVKPICEYCKVIRRNGRVMVICPTNPKHKQRQG"
     gene            complement(2623..2841)
                     /gene="infA"
                     /locus_tag="ACUBV3_000300"
     CDS             complement(2623..2841)
                     /gene="infA"
                     /locus_tag="ACUBV3_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HHU3846836.1"
                     /translation="MAKEDVIEIEGKVVETMPNAMFTVELENGHQILATVSGKIRKNY
                     IRILVGDRVTVEMSPYDLTRGRITYRFK"
     gene            complement(2957..3595)
                     /locus_tag="ACUBV3_000301"
     CDS             complement(2957..3595)
                     /locus_tag="ACUBV3_000301"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HHU3846837.1"
                     /translation="MNLLIMGLPGAGKGTQAAKIVEEFGVAHISTGDMFRAAMANQTE
                     MGRLAKSYIDKGELVPDEVTNGIVKERLAEDDIAEKGFLLDGYPRTIEQAHALDATLE
                     ELGLRLDGVINIKVDPSCLIERLSGRIINRKTGETFHKVFNPPVDYKEEDYYQREDDK
                     PETVKRRLDVNIAQGEPILEHYRKLGLVTDIEGNQEITEVFADVEKALLELK"
     gene            complement(3690..4994)
                     /gene="secY"
                     /locus_tag="ACUBV3_000302"
     CDS             complement(3690..4994)
                     /gene="secY"
                     /locus_tag="ACUBV3_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HHU3846838.1"
                     /translation="MFLKLLRDALKVKMVRNKILFTIFILLVFRIGTHITVPGINVKS
                     LEQMGELPFLNMLNLVSGNAMRNFSVFSMGVSPYITASIVVQLLQMDILPKFVEWGKQ
                     GEVGRRKLNQATRYISLFLAFVQSIGITAGFNTLSSVALVKTPNVQTYLLIGAILTTG
                     SMVVTWLGEQITDKGFGNGVSMIIFAGIISSIPSAITTIYEDFFVNVRSSAITNSYIF
                     VGILIVAVLAIVFFTTFIQQAEYKIPIQYTKLVQGAPTSSYLPLKVNPAGVIPVIFAS
                     SITTIPSTIIPFFQNGKEIPWLTKLQELLNYQTPVGMIIYAILIILFSFFYTFVQVNP
                     EKTAENLQKNSSYIPSIRPGRETEEYMSSLLKKLATIGSVFLAFISLLPIIAQQALHL
                     SSSIALGGTSLLILIATGIEGMKQLEGYLLKRRYVGFMNTTE"
     gene            complement(5015..5455)
                     /gene="rplO"
                     /locus_tag="ACUBV3_000303"
     CDS             complement(5015..5455)
                     /gene="rplO"
                     /locus_tag="ACUBV3_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004225266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HHU3846839.1"
                     /translation="MKLHELKPAEGSRKVRNRVGRGTSSGNGKTSGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRMPKRGFSNINAKEYALVNLDQLNVFEDGTEVTPVVLKEAGIV
                     RAEKSGVKILGNGELTKKLSVKAAKFSKSAEAAITAKGGSIEVI"
     gene            complement(5580..5759)
                     /gene="rpmD"
                     /locus_tag="ACUBV3_000304"
     CDS             complement(5580..5759)
                     /gene="rpmD"
                     /locus_tag="ACUBV3_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HHU3846840.1"
                     /translation="MAQIKITLTKSPIGRKPEQRKTVVALGLGKLNSSVVKEDNAAIR
                     GMVNAISHLVTVEEA"
     gene            complement(5774..6268)
                     /gene="rpsE"
                     /locus_tag="ACUBV3_000305"
     CDS             complement(5774..6268)
                     /gene="rpsE"
                     /locus_tag="ACUBV3_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HHU3846841.1"
                     /translation="MAFKDNAVELEERVVAINRVTKVVKGGRRLRFAALVVVGDRNGR
                     VGFGTGKAQEVPEAIRKAVEAAKKNMVEVPMVGTTIPHEVRSEFGGAKVLLKPAVEGA
                     GVAAGGAVRAVIELAGVADITSKSLGSNTPINIVRATVEGLKQLKRAEEVAALRGISV
                     SDLA"
     gene            complement(6287..6643)
                     /gene="rplR"
                     /locus_tag="ACUBV3_000306"
     CDS             complement(6287..6643)
                     /gene="rplR"
                     /locus_tag="ACUBV3_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HHU3846842.1"
                     /translation="MISKPDKNKIRQKRHRRVRGKLSGTADRPRLNIFRSNTGIYAQV
                     IDDVAGVTLASASTLDKEVSNGTKTEQAVVVGKLVAERAVAKGISEVVFDRGGYLYHG
                     RVKALADSARENGLKF"
     gene            complement(6744..7280)
                     /gene="rplF"
                     /locus_tag="ACUBV3_000307"
     CDS             complement(6744..7280)
                     /gene="rplF"
                     /locus_tag="ACUBV3_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HHU3846843.1"
                     /translation="MSRIGNKVITLPAGVEIINKDNVVTVKGPKGELTREFNKNIGIT
                     VEGTEVTVTRPNDSKEMKTIHGTTRANLNNMVVGVSEGFKKALEMRGVGYRAQLQGSK
                     LVLSVGKSHQDEVEAPEGVTFEVPTPTTINVIGINKESVGQTAAYVRSLRSPEPYKGK
                     GIRYVGEFVRRKEGKTGK"
     gene            complement(7390..7788)
                     /gene="rpsH"
                     /locus_tag="ACUBV3_000308"
     CDS             complement(7390..7788)
                     /gene="rpsH"
                     /locus_tag="ACUBV3_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HHU3846844.1"
                     /translation="MVMTDPIADFLTRIRNANQAKHEVLEVPASNIKKGIADILKREG
                     FVKNVEVIEDDKQGIIRVFLKYGQNGERVITNLKRISKPGLRVYTKHEDMPKVLNGLG
                     IAIVSTSEGLLTDKEARQKNVGGEVLAYIW"
     gene            complement(7943..8128)
                     /locus_tag="ACUBV3_000309"
     CDS             complement(7943..8128)
                     /locus_tag="ACUBV3_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HHU3846845.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCEKCGRPHSVYRKFQLCRVCF
                     RDLAYKGQVPGVTKASW"
     gene            complement(8146..8688)
                     /gene="rplE"
                     /locus_tag="ACUBV3_000310"
     CDS             complement(8146..8688)
                     /gene="rplE"
                     /locus_tag="ACUBV3_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HHU3846846.1"
                     /translation="MANRLKEKYTNEVVPALTEKFNYSSVMAVPKVEKIVLNMGVGDA
                     VSNAKNLEKAAAELALISGQKPLITKAKKSIAGFRLREGVAIGAKVTLRGERMYEFLD
                     KLVSVSLPRVRDFHGVPTKSFDGRGNYTLGVKEQLIFPEINFDDVDKVRGLDIVIVTT
                     ANTDEESRELLKGLGMPFAK"
     gene            complement(8712..9017)
                     /gene="rplX"
                     /locus_tag="ACUBV3_000311"
     CDS             complement(8712..9017)
                     /gene="rplX"
                     /locus_tag="ACUBV3_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HHU3846847.1"
                     /translation="MFVKKGDKVRVIAGKDKGTEAVVLKALPKVNKVVVEGVALIKKH
                     QKPNNENPQGAIVEKEAPIHVSNVQVLDKNGVAGRVGYKVVDGKKVRYNKKSGEVLD"
     gene            complement(9097..9465)
                     /gene="rplN"
                     /locus_tag="ACUBV3_000312"
     CDS             complement(9097..9465)
                     /gene="rplN"
                     /locus_tag="ACUBV3_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HHU3846848.1"
                     /translation="MIQQETRLKVADNSGAREILTIKVLGGSGRKFANIGDVIVASVK
                     QATPGGAVKKGDVVKAVIVRTKTGARRPDGSYIKFDDNAAVIIRDDKTPRGTRIFGPV
                     ARELREGGYMKIVSLAPEVL"
     gene            complement(9490..9750)
                     /gene="rpsQ"
                     /locus_tag="ACUBV3_000313"
     CDS             complement(9490..9750)
                     /gene="rpsQ"
                     /locus_tag="ACUBV3_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HHU3846849.1"
                     /translation="MERNQRKTLYGRVVSDKMDKTITVVVETKRNHPVYGKRINYSKK
                     YKAHDENNVAKEGDIVRIMETRPLSATKRFRLVEVVEKAVII"
     gene            complement(9776..9982)
                     /gene="rpmC"
                     /locus_tag="ACUBV3_000314"
     CDS             complement(9776..9982)
                     /gene="rpmC"
                     /locus_tag="ACUBV3_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HHU3846850.1"
                     /translation="MKLQEIKDFVKELRGLSQEELAKKENELKKELFDLRFQAAAGQL
                     EKTARLDEVKKQIARVKTVQSEMK"
     gene            complement(9992..10405)
                     /gene="rplP"
                     /locus_tag="ACUBV3_000315"
     CDS             complement(9992..10405)
                     /gene="rplP"
                     /locus_tag="ACUBV3_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003029477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HHU3846851.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGCKEVSFGEYGLQATTSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKV
                     MFEIAGVSEEVAREALRLASHKLPVKCKFVKREAE"
     gene            complement(10409..11062)
                     /gene="rpsC"
                     /locus_tag="ACUBV3_000316"
     CDS             complement(10409..11062)
                     /gene="rpsC"
                     /locus_tag="ACUBV3_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HHU3846852.1"
                     /translation="MGQKIHPIGMRVGIIRDWDAKWYAEKEYADYLHEDLAIRKFINK
                     ELADASVSTIEIERAVNKVIVSLHTAKPGMVIGKGGANVDALRGQLNKLTGKQVHINI
                     IEIKQPDLDAHLVGENIARQLEQRVAFRRAQKQAIQRTMRAGAKGIKTQVSGRLNGAD
                     IARAEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEVLPARKNTKGGK"
     gene            complement(11075..11419)
                     /gene="rplV"
                     /locus_tag="ACUBV3_000317"
     CDS             complement(11075..11419)
                     /gene="rplV"
                     /locus_tag="ACUBV3_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HHU3846853.1"
                     /translation="MAEITSAKAMARTVRVSPRKTRLVLDLIRGKNVADAIAILKFTP
                     NKAARVIEKTLNSAIANAENNFGLEKANLVVSETFANEGPTMKRFRPRAKGSASPINK
                     RTTHVTVVVSEK"
     gene            complement(11435..11713)
                     /gene="rpsS"
                     /locus_tag="ACUBV3_000318"
     CDS             complement(11435..11713)
                     /gene="rpsS"
                     /locus_tag="ACUBV3_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HHU3846854.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQANDEKKKVIKTWSRRSTIFPSF
                     IGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYKGHAADDKKTRR"
     gene            complement(11812..12645)
                     /gene="rplB"
                     /locus_tag="ACUBV3_000319"
     CDS             complement(11812..12645)
                     /gene="rplB"
                     /locus_tag="ACUBV3_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019790357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HHU3846855.1"
                     /translation="MGIKVYKPTTNGRRNMTSLDFAEITTNTPEKSLLVSLKNKAGRN
                     NNGRITVRHQGGGHKRHYRLIDFKRNKDGVEAVVKTIEYDPNRTANIALVHYTDGVKA
                     YILAPKGLEVGQRIISGPEADIKVGNALPLANIPVGTVIHNIELQPGKGAELIRAAGA
                     SAQVLGQEGKYVLVRLQSGEVRMILGTCRATIGTVGNEQQSLVNIGKAGRNRWKGVRP
                     TVRGSVMNPNDHPHGGGEGKAPVGRKAPSTPWGKPALGLKTRNKKAKSDKLIVRRRNQ
                     K"
     gene            complement(12663..12959)
                     /locus_tag="ACUBV3_000320"
     CDS             complement(12663..12959)
                     /locus_tag="ACUBV3_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HHU3846856.1"
                     /translation="MNLYDVIKKPVITEKSMVALEAGKYTFEVDTRAHKLLIKQAVEA
                     AFDGVKVASVNTVTVKPKAKRVGRYTGFTSKTKKAIITLTADSKAIELFAAEAE"
     gene            complement(12959..13582)
                     /gene="rplD"
                     /locus_tag="ACUBV3_000321"
     CDS             complement(12959..13582)
                     /gene="rplD"
                     /locus_tag="ACUBV3_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HHU3846857.1"
                     /translation="MANVKLFDQTGKEVSSVELNEAIFGIEPNESVVFDVVISQRASL
                     RQGTHAVKNRSAVSGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYGYKLP
                     QKVRRLALKSVYSAKVAEDKFVAVENLSFAAPKTAEFASVLSALSIDSKVLVILEEGN
                     EFAALSARNLPNVTVATATTASVLDIVNADKLLVTKEAISTIEGVLA"
     gene            complement(13606..14232)
                     /gene="rplC"
                     /locus_tag="ACUBV3_000322"
     CDS             complement(13606..14232)
                     /gene="rplC"
                     /locus_tag="ACUBV3_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HHU3846858.1"
                     /translation="MTKGILGKKVGMTQIFTESGEFIPVTVIEATPNVVLQVKTVETN
                     GYEAVQVGFDDKREVLSNKPAKGHVAKANTAPKRFIREFKNIEGLEVGAELSVEQFEA
                     GDVVDVTGTSKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRLAG
                     RMGGNRVTVQNLEIVQVIPEKNVVLIKGNVPGAKKSLITIKSAVKAAK"
     gene            complement(14337..14645)
                     /gene="rpsJ"
                     /locus_tag="ACUBV3_000323"
     CDS             complement(14337..14645)
                     /gene="rpsJ"
                     /locus_tag="ACUBV3_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HHU3846859.1"
                     /translation="MANKKIRIRLKAYEHRTLDTAAEKIVETATRTGATVAGPVPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLVDIINPTQKTVDALMKLDLPSGVNVEIKL
                     "
     gene            complement(14851..16131)
                     /locus_tag="ACUBV3_000324"
     CDS             complement(14851..16131)
                     /locus_tag="ACUBV3_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001270709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HHU3846860.1"
                     /translation="MRYSKEIIQLAIPAMIENILQMLMGVVDNYLVAQLGVVAVSGVS
                     VANNIITVYQAIFIALGASIASLLAKSLAGSEKDDAISVCSQAIFLTLLIGAVLGIIS
                     IVFGQTFFKLLGTTKSVAQVGGLYLAIVGGGVVTLGMLTTLGSFLRVQGQPRLPMYVS
                     IFVNFLNAVLSGFAIFEWRYGLVGVAVSTLIARLIGICILAKYLPIKKIIKRMTWKIS
                     AQIWNLALPSAGERLMMRAGDVVIVAIVVQLGTNVVAGNAIGETLTQFNYMPGLGIAT
                     ATIILTAKYVGQKNRESIEETIQSSYYIGLVLMILISSFMLLAGKPLTQLFTNNPSAI
                     KGSLIVILLSFVGVPATIGTLVYTAAWQGLGNAKLPFYTTTIGMWLIRVVLGYLLGIV
                     FELGLLGVWMATIADNIFRWLFLKVHYHRYIQKM"
     gene            complement(16236..17726)
                     /gene="thrC"
                     /locus_tag="ACUBV3_000325"
     CDS             complement(16236..17726)
                     /gene="thrC"
                     /locus_tag="ACUBV3_000325"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HHU3846861.1"
                     /translation="MAIVYQSTRDASNKVTASQAILKGLADDGGLFTPITFPKVDLDF
                     TKLKDASYQEVAKLVLSAFFDDFTEQELDYCISQAYDTKFDTTEIAPIVKIGDRYHLE
                     LFHGPTIAFKDMALSILPYLLTTAAKKQGVDDKIVILTATSGDTGKAAMAGFADVPGT
                     EIIVFYPKNGVSYIQELQMITQAGQNTHVVAIEGNFDDAQTSVKEMFNNSLLRSKLSQ
                     QHMQLSSANSMNMGRLVPQIVYYIYAYAQLVKSKEISIGQPINFSVPTGNFGNILAAY
                     YASQIGLPVTKLICASNDNNVLTDFFKTQTYDKNREFKVTSSPSMDILVSSNLERLIF
                     HLLGDDAETTKKLMEDLVTTGEYALEARQANILESFVAGFATEQFVELDIKRLFDQYQ
                     YIEDPHTAVASAVYQAYQTETKDQTPTVIVSTASPYKFPSVVTKAITNKEEIQDFAAI
                     SILNDLSGVSLPKAVTDLQKAEVIHRTVVPTSNMRETVEECLGLKQ"
     gene            complement(17847..18863)
                     /gene="adhP"
                     /locus_tag="ACUBV3_000326"
     CDS             complement(17847..18863)
                     /gene="adhP"
                     /locus_tag="ACUBV3_000326"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HHU3846862.1"
                     /translation="MKAVVVNQASTGVEVVEHDLPKVGHGEALVKVEYCGVCHTDLHV
                     AHGDFGQVPGRILGHEGIGIVEEIGEGVTSLRVGDRVSIAWFFEGCGHCEYCTTGRET
                     LCRSVKNAGYSVDGGMSEYAVVTADYAVKVPEGLDPAQASSITCAGVTTYKAIKEAGA
                     APGQWIAVYGAGGLGNLAVQYAKKVFNAHVVAVDINADKLQLAKEVGADLTVNGKEIK
                     DVAAYIQEKTGGCHGVVVTAVSKVAFNQAIDSVRAGGTVVAVGLPSEYMELSIVKTVL
                     DGIRVVGSLVGTRKDLEEAFAFGAEGLVVPVVEKVPVDTAPQVFDEMERGLIQGRKVL
                     DFTK"
     gene            complement(19043..21685)
                     /gene="adhE"
                     /locus_tag="ACUBV3_000327"
                     /gene_synonym="adhC"
     CDS             complement(19043..21685)
                     /gene="adhE"
                     /locus_tag="ACUBV3_000327"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HHU3846863.1"
                     /translation="MTEKTKAVETTDVALAIDTLVQNGLKALDEMRQLNQEQVDYIVA
                     KASVAALDAHGELALHAVEETGRGVFEDKATKNLFACEHVVNNMRHTKTVGVIEEDDV
                     TGLTLIAEPVGVVCGITPTTNPTSTAIFKSLISLKTRNPIIFAFHPSAQESSAHAARI
                     VRDAAIAAGAPENCVQWIEQPSIDATNALMNHDGIATILATGGNAMVKAAYSCGKPAL
                     GVGAGNVPAYVEKSANIRQAAHDIVMSKSFDNGMVCASEQAVIIDKEIYKEFVEEFKS
                     YHTYFVNKKEKALLEEFCFGAKANSKNCAGAKLNPNIVGKSAVWIAEQAGFTVPEGTN
                     ILAAECTEVSEKEPLTREKLSPVIAVLKAESTEDGVEKARQMVEFNGLGHSAAIHTKD
                     ADLAREFGTRIRAIRVIWNSPSTFGGIGDVYNAFLPSLTLGCGSYGRNSVGDNVSAIN
                     LLNIKKVGRRRNNMQWFKVPSKTYFERDSIQYLQKCRDVERVMIVTDHAMVELGFLDR
                     IIEQLDLRRNKVVYQIFAEVEPDPDITTVMKGTDLMRTFKPDTIIALGGGSPMDAAKV
                     MWLFYEQPEVDFHDLVQKFMDIRKRAFKFPELGKKTKFVAIPTTSGTGSEVTPFAVIS
                     DKANNRKYPIADYSLTPTVAIVDPALVMTVPGFIAADTGMDVLTHATEAYVSQMANDY
                     TDGLALQAIKIVFDYLERSVKDADFEAREKMHNASTMAGMAFANAFLGISHSMAHKIG
                     AQFHTVHGRTNAILLPYVIRYNGTRPAKTATWPKYNYYRADEKYQDIAKLLGLPAATP
                     EEAVESYAKAVYDLGTRLGIKMNFRDQGIDEKEWKEKSRELAFLAYEDQCSPANPRLP
                     MVDHMQEIIEDAYYGYEERPGRRK"
     gene            complement(21954..23732)
                     /locus_tag="ACUBV3_000328"
     CDS             complement(21954..23732)
                     /locus_tag="ACUBV3_000328"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HHU3846864.1"
                     /translation="MRIKWFSLVRITGLLLVLLYHFFKNSFPGGFVGVDIFFTFSGFL
                     ITALLIDEFSKTKKIDFVSFCRRRFYRIFPPLVLMVLVTIPFVFLVKSDFRASIGSQI
                     MTALGFTSNFYEILTGGNYESQFIPHLFVHTWSLSIEVHFYVLWGLTVWLLSKRSKDQ
                     KQLRGTLFLISMGVFGVSFLTMFVRAFFVDNFSTIYFSTLSHIFPFFLGAMVATISGI
                     REITGRFKKNIKNLTLKHNLIMMGSAFAGLMILTFALDFDNRLTYLFGFVLSSIFASV
                     MIYNARILHEHTPDISEPFVITYLADISYGMYLFHWPFYIIFSRLSPNWIAVILTVVL
                     SAVFSTLSFYIIEPFILGRKPKFLDYEFDLLPYKKWLFSIGGVLTLITVVTMLTAPSI
                     GSFETELLQNSLQQARTNTNRTHTLAAGDAGALSDVTVIGDSVALRSSAAFNKLLPEV
                     QLDAAVSRNFSKSFDIFENRIQNKALSKIVVLAVGVNSLDNYKTDLSQFIKSLPKGHR
                     LIIVTPYNAKNMSQVTTVRDYELSLMKKYNYITVADWYKVATEHPEIWGNTDGVHYSD
                     SDTTGADLYVSNVKKAIQKSAQRAAK"
     gene            complement(23729..24109)
                     /locus_tag="ACUBV3_000329"
     CDS             complement(23729..24109)
                     /locus_tag="ACUBV3_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3846865.1"
                     /translation="MKNFKITRTHLEILSLIIIVVFGLSVFTLTTSSQGVLSYDGGKI
                     KYVGSIVNHHMTGKGKLTYENGDYYKGDFVNGVFEGKGTFVSVHGWSYTGDFKKGQPD
                     GQGRLNAKNKKVYKGTFKQGIYQK"
     gene            complement(24116..24553)
                     /locus_tag="ACUBV3_000330"
     CDS             complement(24116..24553)
                     /locus_tag="ACUBV3_000330"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HHU3846866.1"
                     /translation="MKKVCFVCLGNICRSPMAEFVMANLDKEDSLSVESRATSTWEHG
                     NPIHRGTQAILQRYQIPYINDKGSQPISKDDLAYFDYIIGMDEQNVKDLKALSQGTYD
                     DKISLFCDGGVPDPWYTGDFEETYRLVNDGCKQWLTLINNSRD"
     gene            complement(24705..25703)
                     /gene="ruvB"
                     /locus_tag="ACUBV3_000331"
     CDS             complement(24705..25703)
                     /gene="ruvB"
                     /locus_tag="ACUBV3_000331"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001902090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HHU3846867.1"
                     /translation="MTRFLDSDAMGDEELVERTLRPQYLREYIGQDKVKDQLKIFIEA
                     AKLRDESLDHVLLFGPPGLGKTTMAFVIANELGVNLKQTSGPAIEKSGDLVAILNDLE
                     PGDVLFIDEIHRMPMAVEEVLYSAMEDFYIDIMIGAGETSRSVHLDLPPFTLIGATTR
                     AGMLSNPLRARFGITGHMEYYEENDLTEIIERTADIFEMKITYEAASELARRSRGTPR
                     IANRLLKRVRDYAQIMGDGLIDDNITDKALTMLDVDHEGLDYVDQKILRTMIEMYNGG
                     PVGLGTLSVNIAEERDTVEDMYEPYLIQKGFIMRTRTGRVATDKAYEHLGYQRFDK"
     gene            complement(25992..26894)
                     /locus_tag="ACUBV3_000332"
     CDS             complement(25992..26894)
                     /locus_tag="ACUBV3_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000912098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HHU3846868.1"
                     /translation="MLKHFGSKVRNLRVTRNITREDFCGDETELSVRQLARIESGQSI
                     PNLTKAHYIAKQLNVKLDILTGGESLELPKRYKELKYKLLRTPTYGDANRLAVREAYF
                     DEIYEVFYEELPEDERLIIDCMQSKLDVHFSVNDNFGITILHDYFDQIKKKKEYTTND
                     FVMIDLYLLCFSINYGMKSLYSLENYHFIMSKLLEQDNLLPEDNFQLNNVLLNHVELA
                     FQFKQKKYVQQIIHRSNAIMTEIHDFQKRPILSLIEWKYLLIIEKDRTKAETCFKQSI
                     LFAELIGDLYLKGKLIEEWNKDLT"
BASE COUNT         8127 a         5547 c         4544 g         8695 t
ORIGIN      
        1 attttataaa tttagtatag acataactat tcctctatat tcaattaaga gaaattatat
       61 aaccactatt gaaaaatgta gtctagcgat taaactcttt gctcatcgaa aatatccaat
      121 aaatataata cataaaacgc ctgcttacga aatataaaca aaattgtttg catttcgtaa
      181 acaagcgtta cctatttaac aatatatgat gagtgttccc gctgagaata attctcagcg
      241 gtagaccaga gctagactaa gaatcgattg attccatcat cataacactc aacaaaattg
      301 ataaaaatta tactaattca ataattgcca ttggggcagc atcgccacgg cgtggttctg
      361 ttttaagaat acgagtgtat cctccgttac gttcagcata acgaggtgcg atgtcgtcaa
      421 aaagtttttg aagagctgtt gttgatgtat atttatcaga agcttcatca tagttttctg
      481 atgcaatttc attacgtaca taagcagcag cttgacgacg agcatgtaaa tcaccacgtt
      541 tacctagagt aatcattttt tcaactgttt tacggatttc tttagcacgt gcttcagtag
      601 ttacaattga ttcgttgata agaagatcgg ttgtcaaatc acgaagcatt gccttacgtt
      661 gtgagctagt gcgtcctagt ttacggtaag ccattatgtc ctcctatttt atttatcgtt
      721 ttttaatcca agacctagat cggcaagttt aattttaact tcttcaagac tcttacgtcc
      781 taagtttcgg actttcatca tttcaggctc agttttttct gttaaatcaa atactgtatt
      841 aattccagca cgttttaaac agttatatga gcgcactgac aaatcaagtt cctcaattgt
      901 gcggtcaagc actttctcat cgttcacttt ctctgtttcc ttcattactt cagttgcttt
      961 agcaacctct gttaaatcag taaacaagtt taagtgttca attaagacgc gagctgaaag
     1021 accaagagca tcctcaggaa tgattgtgcc atttgtcatg atttcaatag ttaatttatc
     1081 aaagccatcg ttactaccta cacgagcagg ttcaacttga taattaactt ttttcactgg
     1141 tgtatagatt gaatctacag ccaatgttcc cactggtgca tcatcttttt tatttccttc
     1201 tgctggaaca taacctcggt ttttagcaac tgtcattgtt gctttcaaac tgtgaccttc
     1261 agcaattgtg aaaagataat ggtcagggtt tacaatttcg atatcactat ctgttaaaat
     1321 atctccggca gtaatttctg caggaccttc aacgtctagt tcgataatct tttcgtcttc
     1381 gacgtaagat ttcacagcaa gtccttttac attaaggatg atttgcatca catcttcacg
     1441 tacacctggg atagtatcaa attcgtgtaa tactccatca attttgattg atgtgactgc
     1501 tgcacctgga agtgaagaca gaagcacacg acgaagagag tttcctagag ttgtaccgta
     1561 gccacgttct agtggttcga tgacaaatct gccgtaatct ttattttcat caatttttgt
     1621 tattattggt ttttcaaact caatcattta gttactccct cttaaacgaa aagttgtgta
     1681 ctataaaatg ctatgattat acacgacgac gttttggagg acgagcacca ttatgtggta
     1741 caggagtcac atcacgaatt gcagttactt caagaccagc tgcagcaagg gcacggatag
     1801 cagattcacg acctgaacca ggacctttta cagtaacttc aacagtttta agaccgtgtt
     1861 cttgtgcaga tttagcagca gcttcagcag ccatttgagc agcaaatgga gtagatttac
     1921 gagaaccttt aaatccaaga gcaccagctg atgaccatgc aagagcgtta ccatgcacat
     1981 ctgtaatcat aacaatagtg ttattgaatg tagcgtgaat atgagcaaca ccagattcga
     2041 tgttcttttt cacacgacgt ttgcgtgttg gtttagccaa ttgttttacc tcctatttta
     2101 ttttttctta cctgcaatcg caacagcttt acctttacga gtgcgagcgt tatttttagt
     2161 attttgtcca cggacaggaa gtccacgacg gtgacggatt ccacggtatg aaccgatttc
     2221 catcaaacgt ttaatattca agtttacctc acgacgtagg tcaccttcaa ctttgattgc
     2281 atcaacttca cgacggatag cgtcctcttg atctggtgtc aaatctttaa cgcggatatc
     2341 ttcagaaata cctgcagctg ctaagatttt tttagatgta gaaagaccaa ttccgtatac
     2401 ataagtaagt gaaattacta cacgtttgtc atttggaata tcaactccag caatacgagc
     2461 cattttatct cctttctata ttatccttga cgttgtttgt gttttggatt tgtcggacaa
     2521 attaccataa cacgaccgtt acgacgaata actttacagt attcgcaaat tggtttaacc
     2581 gatggtctta ccttcatgtt ttaatccctc caattatttc gactatttaa agcggtatgt
     2641 gatgcgtcca cgtgttaaat cgtatgggct catttcaaca gtaacacgat cacctaccaa
     2701 aatacggatg tagtttttac ggatttttcc tgatacagtt gccaaaattt ggtgtccatt
     2761 ttccaactca acagtaaaca ttgcgttagg catagtttca acaactttac cttcaatttc
     2821 aatcacatct tcttttgcca cgcaaaagta ccccctaaat ttcgatttga tgtcctctat
     2881 aaagaggtaa caattataag tcagactaac ttagtatatc attactaatc tacttatgca
     2941 agtagattag cataatttat ttgagttcta gcaacgcttt ttcaacatct gcaaaaactt
     3001 ctgttatttc ttgattacct tcaatatctg taacaagacc aagcttacga tagtgttcaa
     3061 gaataggttc tccttgagca atattaacgt ccaagcgacg tttgacagtt tcaggcttat
     3121 catcttcacg ttgatagtaa tcttcttctt tataatctac tggtgggttg aacactttgt
     3181 ggaaagtttc accagtttta cgattgataa tacgaccact caaacgctct ataagacatg
     3241 atggatccac tttaatatta ataacaccat ctaagcgtag tcctagttct tcaagcgtag
     3301 catctaaggc gtgtgcttgt tcaatagtac gtggatatcc atcaagtaaa aaaccttttt
     3361 ctgcgatatc atcctcagct aagcgctctt ttacaatccc gtttgttact tcatcaggaa
     3421 ccaattcacc tttatcaata taacttttag ctaaacgtcc catttcggtt tgattagcca
     3481 ttgcggcgcg gaacatatcc cctgttgaga tgtgagcaac accaaattct tcaacgatct
     3541 tagctgcttg agtaccttta ccagcaccag gcaaacccat aattaaaaga ttcattatag
     3601 tctccttatt ttgtttttaa ataaatccaa cacagcatgt cggacttatt aaaaaacaaa
     3661 atagaaggtt gacagagtca acctatattc tattctgttg tattcatgaa tccaacatag
     3721 cgtctcttca aaagatagcc ctcgagttgt ttcatacctt caatacccgt tgcaatcaaa
     3781 atgagtaagc ttgtcccacc taacgcaata cttgatgaca aatgcaacgc ttgttgagca
     3841 ataattggta acaaggaaat aaatgctaga aatactgaac caattgttgc taattttttc
     3901 aatagcgatg acatatattc ttctgtttct cgtcctggtc gaatacttgg aatgtaggac
     3961 gaattttttt gaagattctc tgctgtcttt tcagggttta cttgtacaaa agtatagaag
     4021 aaagagaaca gaataatcaa gatagcatag ataatcatac cgactggggt ttgataatta
     4081 agtaattctt ggagtttagt aagccaagga atttctttgc cattttgaaa gaaaggaata
     4141 atcgtacttg gaattgttgt aatcgagcta gcaaagataa cgggaataac accagctgga
     4201 tttactttta gaggaagata tgaacttgta ggagctcctt gaacaagttt tgtatattgg
     4261 attggaattt tgtattcagc ttgttgaata aatgttgtaa aaaatacaat tgctaatact
     4321 gctacaataa gaatcccgac aaagatgtaa gaatttgtaa ttgctgaaga gcgtacattt
     4381 acaaagaaat cttcgtagat agttgtaatt gcacttggaa tggaagaaat gatacccgca
     4441 aagataatca tagacactcc atttccgaat cccttatcag taatttgttc acctagccaa
     4501 gtaaccacca tactacctgt tgtaaggatt gcaccaataa gcaaatatgt ttgtacattc
     4561 ggagttttaa ctaaagcaac acttgataga gtgttaaaac cagcagtaat accaattgat
     4621 tgtacgaatg caaggaacaa agaaatataa cgtgtagctt ggttaagctt acgacgacct
     4681 acctctcctt gttttcccca ttcaacaaac ttcggaagaa tatccatttg aagtagctga
     4741 acaacaatag acgcagtaat gtatgggcta actcccatag agaataccga aaagtttctc
     4801 attgcattac cactaactaa gttaagcata tttagaaatg gaagttcacc catttgttct
     4861 aaactcttaa cattgatgcc cggtactgtg atatgcgttc cgatacgaaa tacaagaagg
     4921 ataaagatag taaataaaat tttatttctt accatcttta ctttgagtgc atctctaagt
     4981 aattttaaaa acataatgag ttacctctca ttaattagat gacttcgatt gaaccacctt
     5041 tagcagtgat agctgcttca gcagatttag aaaacttagc tgctttaaca gacaatttct
     5101 tagttaattc accattacca agaattttaa cgcctgattt ttcagcacgc acgattccag
     5161 cttctttaag aacaactgga gttacttcag taccatcttc aaagacattt aattggtcaa
     5221 ggttcacaag agcatattct tttgcattga tgtttgagaa cccacgtttt ggcatacggc
     5281 ggaacaatgg agtttgtcca ccttcaaaac caaggcgtac tccaccacca ctacgcgctt
     5341 tttgtccttt ttgaccacgt ccagaagttt taccattacc tgaagatgta ccgcgaccca
     5401 cacgattgcg gactttacgt gatccttctg caggttttaa ttcatgaagt ttcattattt
     5461 tttctcctta tttgtaaaat gctaacgcct ttacgagggg aaaggtactc cccgcaaaga
     5521 ctcgcctata cgatttgttt aaatcgcacc aataacaatc agtgcgattt agcatttaat
     5581 taagcttctt caacagttac taagtgagag atagcgttaa ccataccacg gatggcagca
     5641 ttatcttcct taacaactga agaatttaat ttaccaagtc caagggcaac aactgtttta
     5701 cgttgttctg gtttgcgacc gattggagac ttagtcaaag taattttaat ttgagccatt
     5761 ataatcccct ttcttatgct aagtcagaaa cagaaatgcc acgtaaagca gcaacttctt
     5821 ctgcacgttt taattgtttc aagccctcaa cagttgcgcg aacaatgttg attggagtgt
     5881 ttgaaccaag tgatttagat gtaatatctg caacacctgc taattcgatg acggcacgaa
     5941 cagcaccacc agcggcaact ccagcaccct caacagctgg tttcaacaat actttcgctc
     6001 caccaaattc tgaacgaact tcgtgaggaa ttgttgtacc gaccatagga acttcaacca
     6061 tgtttttctt agcagcttca acagctttgc gaatagcttc tggtacttct tgagctttac
     6121 ctgtaccgaa accaacacga ccattacggt caccaacaac tacaagagct gcaaaacgta
     6181 gacgacgtcc acctttaaca acttttgtaa cgcggttaat cgcaacaacg cgctcttcaa
     6241 gttctactgc attatcttta aatgccatta ttaagtgtcc tccctattag aatttcaatc
     6301 cgttttcacg agctgagtca gccaaagctt ttacacgtcc gtgatagaga tatccaccgc
     6361 ggtcaaacac cacttcagaa atacctttag ctactgcgcg ttcagcaaca agtttaccga
     6421 caacaacggc ttgttctgtt tttgttccgt tagaaacttc tttatcaaga gttgatgcac
     6481 ttgcgagcgt tacacccgct acgtcatcaa ttacttgagc gtagatgcct gtattagaac
     6541 ggaaaatgtt caaacgtggg cgatcagcag ttccagagag tttaccgcga acgcgacggt
     6601 ggcgtttttg gcggatttta tttttatctg gtttcgaaat cacaattttc acctcttaaa
     6661 tatattgtta tgtcaaaatg acaatattaa aatgaaagga tagagaattt gcagaaaaga
     6721 ttctacaaaa actatcaatc ctattattta cctgttttac cttctttacg gcgtacgaat
     6781 tcaccaacgt aacgaatacc tttaccttta tatggttctg gtgaacgtaa gctacgaacg
     6841 taagcagcag tttgtccaac gctttctttg ttgataccga ttacgttgat tgttgttgga
     6901 gttggcactt caaaagtaac gccttctgga gcttcaactt cgtcttggtg agacttacca
     6961 acagaaagaa caagttttga tccttgaagt tgtgcacggt aaccaacacc gcgcatttca
     7021 agagcttttt tgaaaccttc agaaacacca acaaccatgt tgttcaagtt cgcacgagtt
     7081 gtaccatgaa ttgttttcat ttcttttgag tcgttaggac gagtaactgt tacttcagtc
     7141 ccttcaacag tgattccaat atttttatta aactcacgag tgagttcgcc tttaggtcct
     7201 tttacagtaa caacattatc cttgttaatg atttcaacac ctgcaggcaa tgtaattact
     7261 ttattaccaa tacgtgacat gtttattttc tcctgttaaa ttgtcaagct gcttgagcaa
     7321 ctagttttca cggggactca tgagaccaca ggattatact gtgactcaat gagcatttta
     7381 ggtaaaattt taccaaatat atgcaagtac ttctccacca acattttttt ggcgagcttc
     7441 tttgtcagtc aaaagacctt cagaagttga tacaattgca ataccaagtc cgttaagaac
     7501 tttaggcata tcttcatgtt tagtataaac acgaagacct ggttttgaaa tacgtttcaa
     7561 gttagtgata acacgttcac cattttgtcc atatttaagg aacacacgga tgatgccttg
     7621 tttgtcatct tcaataactt caacgttttt tacaaaacct tcacgtttaa ggatatcagc
     7681 aatccctttt ttaatgtttg atgcaggtac ttctaacact tcatgttttg cttggttagc
     7741 attacgaata cgagttaaaa agtctgcaat tgggtcagtc ataaccattt tttaattctc
     7801 ctcttattag cagtttgaat ttaatcactt gctaattaat gagtccttac ggacgttata
     7861 agactaggtc ttagtatatt tgcgtttaaa aatcagtgcc attatcaatg acacctttta
     7921 aacacaaagc atcattaatt tattaccaag atgctttagt tacgcctggt acttgacctt
     7981 tataagccaa gtcacggaag caaacacggc aaagttggaa tttgcggtaa actgagtgtg
     8041 gacgtccgca tttttcacaa cgagtgtaag cttgcgtaga gaacttcgct gggcgtttgt
     8101 tcttagcaat catagatttt ttagccaatt tatttacctc ctagattact ttgcaaaagg
     8161 cattccaagg ccttttagca attcacgtga ctcttcatca gtgttagcag tagtaacgat
     8221 aacgatatca agaccacgta ctttatcaac gtcatcaaag ttgatttctg ggaagatcaa
     8281 ttgttcttta actccaagag tgtagtttcc acgtccatca aatgatttcg ttggaacacc
     8341 atggaagtca cgtacacgtg gaagtgaaac agaaactaat ttatccaaga attcgtacat
     8401 acgttcacca cggagggtaa cttttgcacc gatcgctaca ccttcacgaa gacggaagcc
     8461 agcgattgat ttcttagctt tagtaataag tggtttttga cctgaaataa gtgctaactc
     8521 agcagcagct ttctcaagat ttttagcgtt tgatacagca tcaccaacac ccatgttaag
     8581 aacgattttc tcaacttttg gcacagccat aacagatgaa tagttaaatt tttctgtcaa
     8641 cgcaggaaca acttcattag tatatttttc ttttaagcga tttgccatta tgcttctcct
     8701 ttccttcgtg attaatcaag cacttcgcct gattttttgt tgtaacgaac ttttttgccg
     8761 tcaacaactt tgtaaccaac acgtcctgct acaccatttt tatcaagaac ttgaacgttt
     8821 gacacatgga ttggagcttc tttttcaacg atagcgcctt gagggttttc gttatttggt
     8881 ttttgatgtt tcttgatgag ggctacgcct tcaacaacaa ctttgtttac ttttggaaga
     8941 gctttaagaa ctacagcttc agtgcctttg tccttaccag caataacgcg aactttgtcg
     9001 ccttttttta caaacatttg agtttttctc ctatttttct tacgcctcga aaggcaccct
     9061 aggaaatcct aggggactac gtttgttaat ctgagattaa agtacttctg gtgccaatga
     9121 aacgatcttc atgtagccac cttcacgcaa ttcacgtgca actgggccaa agatacgagt
     9181 tccgcgagga gttttatcgt cacggatgat tacagcagca ttgtcgtcaa atttgatgta
     9241 tgaaccgtct gggcggcgtg caccagtttt tgtacgaacg ataacagctt taaccacatc
     9301 acctttttta actgctccac caggagtagc ttgttttaca gaagcaacga ttacgtcacc
     9361 gatgttagcg aatttacgtc ctgaaccacc aagtactttg atagtcaaga tctcacgagc
     9421 accgctatta tcagcaactt tcaagcgagt ttcttgttga atcatttcaa ttttctcctt
     9481 ttagtttgat tagataataa cagctttttc cactacttcc acaagacgga aacgttttgt
     9541 agctgaaagt gggcgagttt ccatgatacg aacgatatcg ccttctttag caacgttgtt
     9601 ttcatcatgt gctttatatt ttttagaata gttgatacgt ttaccataga ctgggtggtt
     9661 acgtttagtt tcaactacaa ctgtgattgt tttgtccatc ttgtcagaca ctacgcgtcc
     9721 gtaaagagtt ttacgttgat tacgttccat ttagaatttc tcctttccca atctattatt
     9781 tcatttctga ttgcacagtt ttaacacgtg caatttgttt cttaacttcg tcaaggcgag
     9841 cagtcttttc aagttgacct gctgcagctt ggaaacgaag atcaaacaat tcttttttga
     9901 gttcgttttc tttcttagca agttcttctt gagacaatcc acgaagctct ttaacaaaat
     9961 ctttaatttc ttgaagtttc atgtcttctc cttattctgc ttcacgtttt acgaatttgc
    10021 atttaactgg taatttgtgg ctagcaagac gtaatgcttc acgtgcaact tcttcagaaa
    10081 caccagcaat ttcaaacatc actttaccac gtttaactgg tgctacccaa ccttcaggtg
    10141 cccctttacc agaacccata cgtacaccga tagcttttgc agtgtatgat ttgtgtggga
    10201 agattttaat ccaaacttta ccaccacgtt tcatgtaacg agtcatcgca atacgggcag
    10261 cttcgatttg acggtttgta atccagtggc tagttgtagc ttgaagtccg tattcaccga
    10321 atgaaacttc tttacaacct ttagcctcac cgcgcatttt tccacggaat tcgcgacggt
    10381 gtttaacacg tttaggtact aacatttgtt atttgcctcc tttagtgttt ttacgagctg
    10441 gaagaacttc tccacggtaa atccaaactt taacgccaag tttaccataa gttgtgtcag
    10501 cttcttccca agcatagtcg atatcagcac gaagcgtgtg aagtggaaca gttccttctg
    10561 aataaccttc agcacgagcg atatcagcac cgttcaaacg accagaaact tgagttttaa
    10621 tacctttagc tcctgcacgc attgtacgtt ggattgcttg tttttgagca cgacggaaag
    10681 caacacgttg ctctaattga cgagcaatat tttcaccaac aaggtgagca tctaaatcag
    10741 gttgtttgat ttcaatgatg ttgatgtgta cttgttttcc agttaatttg ttaagttgac
    10801 cacgaagagc gtcaacattt gctccacctt taccgataac catacctggt ttagcagtgt
    10861 gaagtgaaac aataactttg tttactgcac gttcaatttc aatagttgaa actgatgcgt
    10921 cagctaattc tttattaatg aatttacgga ttgcaagatc ttcatgaagg taatccgcgt
    10981 attctttttc agcataccat ttcgcatccc agtcacggat gattccgaca cgcataccaa
    11041 ttggatgtat tttttgaccc acgattttac ctccttattt ttctgataca actacagtta
    11101 cgtgagttgt acgtttgttg attggtgaag ctgaaccttt cgcacgtgga cggaaacgtt
    11161 tcattgttgg tccttcgtta gcgaatgttt cagatactac caagtttgct ttttccaaac
    11221 caaagttgtt ctctgcattt gcaattgctg agttaagagt tttctcaata acacgagctg
    11281 ctttgtttgg agtgaatttt aagattgcga ttgcgtcagc aacattctta ccacggataa
    11341 gatcaagtac taaacgtgtt ttacgaggtg aaacacgcac tgtacgagcc attgctttag
    11401 ctgaagtaat ttctgccatt gtgtcctcct cctattaacg acgtgttttc ttgtcgtcag
    11461 ctgcgtgacc tttgtaagta cgtgttggtg caaattcacc aagtttgtga cctaccatgt
    11521 cctcttggat ataaacagga acatgtttac gtccatcata aactgcgatt gtgtatccga
    11581 tgaaacttgg gaaaatcgtt gaacgacgtg accaagtttt gattactttt ttcttttcgt
    11641 catttgcttg agcttcaact tttttcatta aatgctcgtc gacgaaaggt ccttttttaa
    11701 gactacgtcc cattttgtag ttttctcctt taaataatgt accacaacgg cctgtacact
    11761 atagtgtact accgagctgg cggatttgta taagattatg ctattaacct attatttttg
    11821 gttacgacga cgaacaataa gtttgtctga tttagctttc ttattacgag ttttaagtcc
    11881 aagtgcaggt ttaccccatg gagtagatgg tgctttacga ccaactggtg ctttaccttc
    11941 accaccaccg tgtgggtgat cattagggtt cattacagaa ccacgaactg tagggcgaac
    12001 acctttccaa cgattacgtc ctgctttacc aatgttaaca agtgattgtt gttcattacc
    12061 aacagtacca attgttgcac ggcaagtacc aaggatcata cgtacttcac ctgattgaag
    12121 gcgaacaaga acatatttac cttcttgacc aagtacttga gcagaagcac ccgcggcacg
    12181 aatcaattca gcacctttac ctggttgcaa ttcaatatta tggataactg taccgactgg
    12241 aatgttagca agtggaagtg cgttaccaac tttgatatct gcttctggac cagaaataat
    12301 gcgttgacct acttcaaggc ctttaggcgc aagaatgtaa gctttaacac catcagtata
    12361 gtgtacaaga gcaatatttg cagtacgatt tggatcgtat tcaattgttt taacaactgc
    12421 ttcaacacca tctttattac gtttgaagtc aatcaaacgg taatgacgtt tgtgtccacc
    12481 accttggtga cgaacagtaa tacgtccgtt gttgttacgt cctgctttat tctttagtga
    12541 aacaagcaat gatttctcag gagtgtttgt agtgatttct gcaaaatcca aagaagtcat
    12601 attacgacgg ccatttgtcg ttggtttata aactttaata cccacgttat ttcctcctta
    12661 gattattcag cttcagcagc aaacaactcg attgcttttg aatcagctgt aagagtgatg
    12721 atagcttttt tagtttttga agtgaaacct gtgtaacgac caacacgttt tgcttttggt
    12781 ttaactgtca cagtgtttac acttgcaact tttactccgt caaatgcagc ttcaacagct
    12841 tgtttgatca aaagtttgtg tgcacgtgtg tcaacttcaa atgtatattt ccctgcttca
    12901 agagcaacca ttgatttctc agtgataaca ggttttttga ttacgtcata caaattcatt
    12961 atgcaagaac cccctcgatt gtagagattg cttctttagt aacaagaagt ttgtctgcgt
    13021 taacgatatc aagaacactt gcagtagttg cagtagcaac tgtaacgttt ggaaggttac
    13081 gcgctgaaag tgcagcaaat tcgttacctt cttcaaggat tacaagtact tttgaatcaa
    13141 tgctaagtgc tgaaagaact gacgcaaatt cagcagtttt tggagctgca aatgaaaggt
    13201 tttctacagc tacaaattta tcttcagcaa cttttgctga ataaactgat ttcaacgcaa
    13261 ggcgacgaac tttttgtgga agtttgtagc cgtatgaacg tggagttggt ccaaagacaa
    13321 caccaccacc acgccattgt ggtgagcgga tagaaccttg acgagcgcgt ccagttcctt
    13381 tttgacgcca tggtttacgt ccaccaccag atactgctga acggttttta accgcgtgag
    13441 ttccttggcg aaggctagcg cgttggctga ttacaacatc aaaaacaact gattcgtttg
    13501 gttcaatacc gaagatagct tcgttaagct caactgagct aacttcttta ccagtttggt
    13561 caaatagttt aacgtttgcc attttaactg ttttctcctt tcttattatt tagcagcttt
    13621 aactgctgat ttgatagtga taagagattt cttagcacct ggtacgttac ctttgatgag
    13681 gacaacgttt ttctctggga taacttgtac aatttcaagg ttttgaactg ttacacggtt
    13741 accacccata cgtcctgcca agcgtttgtt tttgaaaacg cggttaggcg caacaggtcc
    13801 catagaacct ggacggcgat ggtaacgaga accgtgagcc ataggaccac gtgattgacc
    13861 atggcgtttg ataacacctt ggaaaccttt accttttgat gtccctgtga cgtcaacaac
    13921 atcaccagct tcaaattgtt ctacagataa ttctgcacca acttctaagc cttcaatgtt
    13981 tttgaattca cgaatgaagc gcttaggagc tgtgtttgct tttgcaacat ggcctttggc
    14041 aggtttgtta ctcaatactt cacgtttgtc atcaaaacca acctgaactg cttcgtaacc
    14101 gtttgtttca acagttttaa cttgaagcac aacgtttgga gttgcttcaa tgacagtaac
    14161 agggatgaat tcgcctgatt cagtgaagat ttgagtcatt cccacttttt tccctaagat
    14221 tccttttgtc atgagaaaat atttcctttt cttattttat ttcaaaaagt tttttacgag
    14281 cgttttttgt gctcaaaaaa gttttttacg agcgtttttt gtgctcagaa atcaaattaa
    14341 agtttgattt ctacgttgac accacttgga agatcaagtt tcatcaaagc atcaacagtt
    14401 ttttgtgttg ggttgatgat gtctaccaaa cgtttgtgtg tacgcatttc aaactgttcg
    14461 cgagaatctt tatatttgtg agtcgcacga ataattgtgt aaagactgcg ttcagttgga
    14521 agcggaactg gtccagcaac tgtagcacct gtacgtgttg cagtttcaac gattttttct
    14581 gccgctgtat caagtgtacg gtgttcgtac gccttcaaac ggatacggat ttttttgttt
    14641 gccatctttt ctcctctcgt ctatttacga taataggcta gctcctcaag aaaaccaaca
    14701 cgagttgcgt ggcaatgcaa cctagcgtgt cgcaacctct tgcatcatag ctaaagctgt
    14761 tcatacacag caccagtata tgatatcaaa aaaaaacagc acacgcaagc attatttttt
    14821 tattttcgaa aaaaatgcaa ccatttctgg ttacattttt tgaatatatc gatgatagtg
    14881 aactttcaaa aacagccacc taaagatatt atctgctatc gtagccatcc aaaccccaag
    14941 caaacctaat tcaaaaacta ttcctaataa ataaccaaga actactctga ttaaccacat
    15001 gcctatagtc gttgtgtaaa atgggagttt agcatttcct aatccttgcc aagctgctgt
    15061 ataaaccaag gtcccaatag tagcaggtac tcctacgaaa gatagcaaaa taacaatcaa
    15121 acttccttta atggcagaag gattatttgt aaataactgt gtcaatggtt tgcctgcaag
    15181 aagcataaaa cttgatataa ggatcattag aactaagcca atataatacg aactttgaat
    15241 agtttcttct atagattctc tatttttttg tcctacatat tttgcagtta ggataattgt
    15301 agcagttgcg atgccaagcc ctggcatata attaaattgt gtcaaggttt ctccaatagc
    15361 atttcccgct acaacatttg tacctaactg aacaacaata gcaactataa caacatctcc
    15421 agctcgcatc atcagacgtt ctcctgctga tggtaaggca agattccaaa tttgtgcact
    15481 gattttccaa gtcattcttt ttattatctt tttaatagga agatacttcg caaggataca
    15541 aataccaatt aatcttgcga taagagttga tactgccact ccgaccaaac catagcgcca
    15601 ctcaaaaata gcaaaacctg ataagactgc attgaggaaa ttgacaaaaa tactgacata
    15661 cattggtaaa cgaggttgcc cttgcacacg taaaaatgag cctagggtgg ttaacattcc
    15721 taaagtaaca acacctccac ccacaattgc taaatataaa ccaccaactt gtgcgacact
    15781 ttttgttgtc cctaaaagtt taaagaaagt ttgtccaaaa acaatcgaga taattcctaa
    15841 tactgcccct atcagtaatg ttagaaaaat ggcttgagaa catactgaaa ttgcatcatc
    15901 cttctcacta cctgctaacg acttggccaa tagacttgct atactcgccc ctaaagctat
    15961 aaaaatagct tgataaacag taattatatt attagcaact gaaacacctg atactgctac
    16021 aacacctaac tgagccacta gataattatc aactactccc atgagcattt gtaagatatt
    16081 ttcaatcata gctggtatag ctaactgaat aatctcttta ctataacgca cagtatctcc
    16141 tttctattac ccaggatcat taaaaagaga gtgatgggaa aatttctaaa atgtttcttt
    16201 ctcgctctaa atattttaaa acaatcgtat ttttattatt gtttcaaacc tagacactct
    16261 tcaaccgttt ctcgcatatt tgaggtaggt actaccgttc tatggattac ttcggccttt
    16321 tgtaaatctg taaccgcctt aggtaaagaa acacctgaca aatcgtttaa gattgaaata
    16381 gcagcgaaat cctgtatctc ttctttattt gtaatagcct ttgtaacaac actcgggaac
    16441 ttataaggac ttgctgttga cacaattact gttggagttt gatctttagt ttctgtctga
    16501 taagcttgat aaacagctga tgctacagct gtatgcggat cctctatata ctgatactgg
    16561 tcaaataaac gtttgatgtc taattcaaca aattgttctg tcgcaaatcc cgctacaaag
    16621 ctctctaaaa tatttgcttg cctagcctct aaggcatact ccccagtagt cactaaatct
    16681 tccattaatt ttttagtcgt ttctgcatca tcgcctaaaa ggtgaaagat taatcgttct
    16741 agattagagg agaccaaaat atccatagag ggacttgatg tcactttaaa ttctcgattt
    16801 ttatcgtagg tttgtgtctt aaaaaaatct gttagaacgt tattatcatt tgaggcacaa
    16861 atgagtttgg taacaggtaa tccaatttga ctagcataat atgctgctaa aatattacca
    16921 aaatttcctg taggtacact aaaattaatt ggttgaccaa ttgagatttc ctttgactta
    16981 actaattgag catatgcata tatatagtaa acaatttgag gaacaaggcg acccatgttc
    17041 attgaattgg ctgatgataa ctgcatatgc tgctgtgata attttgatcg aagaagggaa
    17101 ttattgaaca tttctttaac tgaagtctgt gcatcatcaa aatttccttc aatcgcaaca
    17161 acatgggtat tttgtccggc ttgtgttatc atctgcaatt cttggatata actaacacca
    17221 tttttaggat aaaagacaat aatctcagtt cctggaacat ctgcaaaacc tgccatcgct
    17281 gccttaccag tgtcacctga tgttgctgtt aaaataacga ttttatcatc aaccccttgt
    17341 tttttggctg ctgttgttag caaataagga agaatggata aagccatatc tttaaatgca
    17401 attgtaggac cgtgaaataa ttcaaggtga taccggtctc caatctttac tattggggct
    17461 atttcagttg tatcaaattt agtatcataa gcttgactaa tacagtaatc caattcttgt
    17521 tcagtaaaat catcaaaaaa agctgacaaa acgagtttag ccacttcttg gtaggaagca
    17581 tccttaagtt tagtgaaatc taaatcaact tttggaaaag tgatgggggt aaagagtcct
    17641 ccatcgtcag ctaatccctt tagaatcgct tgactagctg tcactttatt actagcatca
    17701 cgtgttgatt gatatactat tgccataaga atccctcttt atataaatat cttttagtta
    17761 ttgtaacaca atttaataac atgtccattg ttcgttatat aattaacccc cctttattaa
    17821 aataaagggg gggcgtaata tcattatcac tttgtaaagt cgagaacttt acgtccttga
    17881 atcaatcctc tttccatttc atcgaatact tgaggagctg tatccacagg cactttttca
    17941 accactggta ctaccaaacc ttctgctcca aatgcgaagg cttcttctaa atctttacga
    18001 gttccaacta aggaaccaac cacacgaatc ccatcaagta ctgtttttac aatactcaat
    18061 tccatatatt ctgatggaag accaacagct accactgtac ctccagcacg aacgctgtca
    18121 atcgcctgat taaacgcaac tttagaaaca gcagtcacaa ccactccatg acaaccgcct
    18181 gtcttttctt gaatataggc cgcaacatct tttatttctt tgccattaac tgtcaaatct
    18241 gctccaacct ctttagctaa ttgaagttta tctgcgttaa tatcaacagc tacaacatga
    18301 gcattgaata ccttttttgc atattggact gctaagtttc caagaccacc tgcaccatac
    18361 actgcaatcc actgaccagg agcagctcca gcttctttga tagccttgta tgttgttact
    18421 ccagcacaag tgattgatga tgcttgagct ggatctaatc cctcaggaac cttaaccgca
    18481 tagtccgcgg taacgacagc gtattcactc ataccaccat caacactgta tccagcattt
    18541 ttaacactac gacaaagcgt ctcacgtcct gtagtacagt attcgcaatg accgcaccct
    18601 tcaaagaacc atgcaataga gacacgatca ccaaccctca aagacgttac gccttctcca
    18661 atttcttcta ctataccaat gccttcgtga cccaaaatgc gtcctggtac ctggccgaaa
    18721 tctccatgtg caacgtgtaa atcagtatgg cacacaccac agtattctac ttttacaagt
    18781 gcttcaccat gaccaacttt tggaagatca tgctcaacaa cttcaacacc tgtactagct
    18841 tgattaacaa caaccgcttt catgataaaa cctcctagaa ataaatatat cttattttgt
    18901 aatcgtttac attttatatt gtattattct tcacaaacta tgtcaagtga ttttcagaaa
    18961 attttttgaa aaatattctt aactggttaa tctttttgaa actaaaaatc ccactcaata
    19021 gagtgagatt cttagactgc tgttacttac gacgtcctgg acgttcctca taaccataat
    19081 aagcatcttc gatgatttct tgcatgtgat ctaccatcgg aaggcgtgga tttgctggtg
    19141 aacattgatc ttcatatgct aagaaagcta gttcacgaga tttttctttc cattcttttt
    19201 catcaatgcc ttggtcgcgg aaattcatct taatacctaa acgtgttcca aggtcataca
    19261 cagcttttgc atatgattcg acagcttctt ctggtgttgc tgctggtaag ccgagtaatt
    19321 tagcgatatc ttgatatttt tcatcagcac gatagtaatt gtacttaggc catgttgctg
    19381 ttttagctgg acgagttcca ttatagcgga taacatacgg caaaagaatt gcatttgtac
    19441 gtccgtgtac ggtatggaat tgtgcgccaa ttttatgcgc cattgagtga gaaataccta
    19501 agaatgcatt ggcgaaagcc atacctgcca tagttgacgc attgtgcatc ttttcacgtg
    19561 cttcaaaatc agcatctttt actgagcgtt ctaagtagtc aaacacaatt ttaattgctt
    19621 gaagggcaag tccgtctgta taatcatttg ccatctgtga aacataagcc tctgttgcat
    19681 gtgtcaatac atccataccc gtatcagccg caataaaacc aggaacagtc ataacaaggg
    19741 ccggatcaac gatagctaca gttggtgtta atgaataatc agcgattgga tatttacgat
    19801 tatttgcttt gtcagagata acggcaaatg gtgtcacttc agaacctgtt ccagaagttg
    19861 ttggaatagc aacaaatttt gttttcttac caagttctgg gaatttgaac gcacgtttac
    19921 ggatatccat gaatttttga acgaggtcat ggaaatcaac ttctggttgt tcatagaaga
    19981 gccacataac tttcgcagca tccataggag aaccaccacc taatgcaata atagtgtctg
    20041 gtttgaaggt acgcatcaaa tctgttcctt tcattactgt tgtaatgtct ggatctggtt
    20101 ctacttcagc aaaaatctga taaacaactt tattgcgacg aagatcaagt tgttcaatga
    20161 tacgatccaa gaaaccaagc tcaaccatgg cgtgatctgt aacaatcatg acacgttcaa
    20221 catcgcgaca tttttgcaaa tattgaatag aatcacgttc gaagtatgtt tttgaaggga
    20281 ctttaaacca ttgcatatta tttctacgtc ttcctacttt tttgatattc aagagattta
    20341 tagcactaac gttatcacca actgagttac gtccatacga accacaacca agagtcaatg
    20401 atggcaagaa agcattgtaa acatccccaa taccaccaaa agtagaagga gaattccaaa
    20461 taacacggat agcacgaatt ctagttccaa attcacgagc taagtctgca tcttttgtat
    20521 gaatagcagc tgagtgtcct agaccattaa actcaaccat ttgacgagct ttttcaacac
    20581 catcttctgt agattcagct ttcaatacgg caataacagg tgatagtttt tcacgtgtca
    20641 aaggttcttt ttcactaact tcagtacatt ctgcggcaag aatatttgtt ccttctggaa
    20701 cagtaaatcc agcttgttca gcaatccaaa ctgctgattt tccaacaata tttggattta
    20761 attttgcacc agcacagttt ttgctatttg cttttgcacc aaaacagaat tcttcaagaa
    20821 gagctttttc tttcttatta acgaaataag tatgataaga tttaaattct tcaacaaatt
    20881 ctttataaat ttctttatca ataataacag cctgttctga tgcacatacc ataccgttat
    20941 caaatgattt agacataaca atatcgtgag cggcttgtcg gatattagct gatttttcaa
    21001 cataagctgg aacattccca gcacctacac caagggcagg tttcccacat gagtaggcag
    21061 ctttaaccat cgcattacca cctgtcgcaa ggatagtggc aataccatca tggttcatca
    21121 aagcattagt tgcatcaatc gatggttgtt caatccattg gacacaattt tcaggagctc
    21181 cagcagcaat agcagcatca cgaacaatac gtgctgcatg tgctgatgat tcttgtgctg
    21241 atgggtggaa ggcaaaaata attggattac gtgttttaag agagattaat gacttgaaaa
    21301 tagcagttga agtcgggttg gttgttggcg taataccaca gacaactccc actggttcag
    21361 caattaatgt caagcctgtg acatcgtctt cttcaataac tcccactgtc tttgtatgac
    21421 gcatgttgtt aacgacgtgt tcacatgcaa ataaattttt tgtcgcttta tcttcaaaga
    21481 caccacgacc agtttcttct actgcatgaa gagcaagttc tccatgggca tccaaggctg
    21541 caacagaagc tttagctaca atataatcaa cttgttcttg attaagttga cgcatctcat
    21601 caagtgcttt taaaccgttt tgaaccaatg tatcaattgc aagagcaaca tctgtagttt
    21661 ctaccgcttt agttttttca gtcatttgag actccttttg atttgttaaa tgtttcacaa
    21721 atttgtttac gagattatta tagcatattt aaaaactttg taaatagttt cacaactttt
    21781 ttgtgaactt ttttccaatc tttcggaaac atttcttgaa agcgctttaa ttgaacttgt
    21841 tttttcatta ctaattttca gattaaagcc aatcgatttc atttgtatca ccataaacgg
    21901 aggtatctat tattcgtttt cacaaaaaag ccgagtacat gactcggact tgtttatttt
    21961 gcagctcgtt gagctgattt ttgaatagct tttttgacgt tactaacata taaatcagcc
    22021 cctgtagtat cgctatcact ataatgcaca ccatctgtat ttccccagat ttctggatgt
    22081 tctgtcgcaa ccttatacca gtcagcaacc gttatgtaat tatatttttt cattagcgag
    22141 agttcataat cacgcactgt cgtaacttga ctcatatttt tagcattata aggcgttact
    22201 ataatcaaac ggtgtccctt aggcaaagat ttaataaatt ggctcaagtc tgtcttgtag
    22261 ttatctaatg aattcacacc aactgccaaa acaacaattt tggacaaggc tttattttga
    22321 atacggtttt caaaaatatc aaatgattta ctaaaattac gactcaccgc agcatctaat
    22381 tgtacttctg gtaacaattt attaaaagca gcacttgacc ttaaggcaac tgaatctccg
    22441 attaccgtca catcactaag agcacctgca tcccctgcgg ctagtgtatg tgttcggtta
    22501 gtatttgttc tagcttgttg taacgaattc tgcaataatt ctgtctcaaa acttcctata
    22561 cttggagctg tcaacattgt cactacagta ataagagtta gtacccctcc gatactaaat
    22621 aaccactttt tgtaaggcaa caagtcaaat tcataatcaa ggaatttagg ttttctacct
    22681 aaaataaaag gttcaatgat ataaaacgat aatgttgaga agactgctga taatactact
    22741 gtcaaaataa cagctatcca gtttggggat aaacgactaa aaataatata aaatggccaa
    22801 tgaaaaaggt acataccgta actgatatca gcaagataag taatcacgaa aggttctgaa
    22861 atatctggtg tatgctcgtg taaaatacgt gcattgtaaa tcattacact agcaaaaatg
    22921 ctagacaaaa caaaaccaaa aagatatgtt agccgattat caaaatcaag tgcaaaagtt
    22981 aaaatcatta gaccagcaaa agcactaccc atcatgatta aattatgctt caaagtcaga
    23041 tttttgatat ttttcttgaa tcgtccggta atctctcgaa taccagatat tgtagccacc
    23101 atcgctccta aaaagaacgg aaatatatgt gataatgtag agaaatatat cgttgaaaaa
    23161 ttatcaacga aaaaggctct aacaaacatg gtaaggaagc tgactccaaa aactcccatg
    23221 gaaataagga acaaagtccc cctcaattgc ttttgatctt ttgatctttt cgataagagc
    23281 cataccgtta agccccataa aacataaaaa tggacttcaa tagaaagact ccaggtatgc
    23341 acaaataagt gagggataaa ttgactttcg tagttaccac cagtcaagat ttcgtaaaag
    23401 ttgctggtaa aacctaaagc agtcataatt tgagagccaa tacttgctct aaaatcactt
    23461 ttaaccagaa atacaaaagg tattgtcacc aaaaccatca atactaatgg tgggaaaata
    23521 cggtaaaaac gtctacgaca aaaactaaca aaatcaattt ttttcgtttt agaaaattca
    23581 tcaatcaata aggccgtaat cagaaaacca gaaaatgtaa agaagatgtc aacgccaaca
    23641 aatcctcctg gaaaactatt cttaaagaaa tggtaaagta aaacaagtag tagtcctgtt
    23701 atacgtacca aagaaaacca ttttattctc atttttgata aatcccctgt ttaaatgtcc
    23761 ccttatagac tttcttattt ttagcattga ggcgaccttg tccatcaggt tgaccttttt
    23821 taaagtcacc tgtataggac cagccatgaa ctgaaacaaa tgttcctttc ccttcaaaga
    23881 ccccatttac aaaatcgccc ttatagtaat ccccattctc ataggttaat ttacctttac
    23941 cagtcatatg gtgattaaca atagagccca catattttat cttgccgcca tcatagctaa
    24001 ggactccttg actagaagtc gttaaggtaa atactgataa accaaaaaca acaataatta
    24061 tcaaagataa tatttctaga tgagttcgtg ttattttaaa attcttcatt tctacttaat
    24121 ctctactatt atttattagc gttaaccact gtttacagcc atcattgact agacgatagg
    24181 tttcctcaaa atcaccagta taccatggat caggaacacc accatcacaa aaaaggctaa
    24241 tcttatcatc gtaggttcct tgtgatagag cttttaaatc cttgacattt tgctcatcca
    24301 taccgatgat ataatcaaaa tatgctaaat cgtctttaga aataggttgt gaccctttat
    24361 cgttaatgta aggaatttga taacgctgca aaatcgcttg cgttccccta tgtataggat
    24421 ttccatgctc ccatgttgaa gtcgcacgac tctcaaccga gaggctatcc tccttatcta
    24481 aattcgccat aacaaactca gccatcgggc tacggcagat atttccaaga caaacaaaac
    24541 agactttctt catagcaaac tccttttggt ctattataac agaaaaaagg tacttataat
    24601 acgtttaact gctcaaagaa taactacaac atatctaata cttaacattg catgaaaatt
    24661 aaaaaattaa gactagatac cctagcctta aaatgactta tgttttattt atcaaatcgc
    24721 tgataaccta aatgttcata tgccttatcc gtagctacac gaccggtacg ggtacgcata
    24781 ataaaacctt tttgaattaa ataaggttcg tacatgtctt caacagtatc tcgttcttca
    24841 gcaatattaa cggatagagt tcctaaacca acaggacctc cattatacat ttcaatcatg
    24901 gttcttaaga ttttttgatc gacgtaatca agcccctcgt gatcaacatc taacatcgtt
    24961 aatgctttat ctgtaatatt gtcatctatc aaaccatctc ccatgatttg agcataatct
    25021 cgaacacgtt tcaataaacg gttagcgata cgtggcgttc cacgactgcg acgcgctaat
    25081 tcagaagcag cttcataagt aattttcatt tcaaaaatgt ctgctgtacg ctcaataatt
    25141 tctgtcagat cattttcttc ataatactcc atatgccctg taataccaaa gcgagcacgt
    25201 aagggattag ataacatacc tgcacgtgtc gttgcaccaa ttaaggtaaa gggcggcaaa
    25261 tctagatgaa cacttctact agtttctcct gcaccaatca taatgtcaat ataaaagtct
    25321 tccattgcac tatagagcac ctcttcaacc gccatcggca tacgatgaat ttcatcaata
    25381 aaaagaacat cacctggttc taaatcattt aaaatggcta ctaaatcccc tgatttttca
    25441 attgcgggac ctgatgtttg tttgagattg acacccaact cattagcaat tacaaatgcc
    25501 atggttgttt tccctaaacc aggagggcca aataataaca catgatccaa tgactcatca
    25561 cgcaatttag cagcttcaat aaatattttt agctgatctt taaccttatc ttgtccaata
    25621 tactctctta aatactgcgg acgaagtgta cgttctacca attcttcgtc acccattgca
    25681 tcactatcta aaaatcttgt catgatatta ttataacatt tgtataggaa aagttaagaa
    25741 ctaagttggt gtattagtat ctcccttatt taaagaaggt aagtgataca tataatttat
    25801 ctacactaac aaacgataga tagggtggac tatgctaaac tactttaaaa tatttagaag
    25861 tcatagaccc caccacccca ttgttaacaa agttccaaca agtttaataa ctaaagtcat
    25921 ctgatttact ccttacttaa tttgtaaaca cagtatatca atgactgaca tttatctaca
    25981 tgacatcctt gtcatgttaa atctttattc cactcctcta ttaattttcc ttttaaatat
    26041 agatcaccta ttaactctgc aaatagaatt gattgtttaa aacatgtttc tgcttttgtt
    26101 ctatcttttt caataattaa taaatatttc cactctatta agcttagtat tggtcttttt
    26161 tgaaaatcat ggatttctgt cataatagca ttacttctat gaataatttg ctgtacatat
    26221 tttttctgct taaattgaaa agctaattcg acatgattta ataaaacgtt gttcaattga
    26281 aaattatcct caggaagtaa attatcttgc tctaacaatt ttgacataat aaaatgatag
    26341 ttctctaaag aatacaagct cttcattcca tagttaatag aaaagcaaag taaatataaa
    26401 tctatcataa caaaatcatt tgtggtatat tccttttttt tcttaatttg atcaaagtag
    26461 tcatgtaaaa tagtgattcc aaaattatca ttaacagaaa aatgaacatc taattttgat
    26521 tgcatacaat caataattag tctttcatct tctgggagct cctcataaaa tacttcatag
    26581 atttcatcaa aatatgcttc tctaactgca agtctattag catctccgta agtcggggta
    26641 cgcaacaatt tgtacttcaa ctctttatac cgtttaggta attctaagct ctctcctcct
    26701 gttaaaatat ccaattttac attcaattgt ttagctatgt agtgagcttt agtcaagttt
    26761 ggaattgact gtccactttc aatcctagct aattgtctta cagataactc agtttcatca
    26821 ccacaaaaat cttcgcgagt aatatttcta gttactctca aatttcttac tttactacca
    26881 aaatgtttta acattttcct ttaaaaacta act
//
LOCUS       DBJYSH010000009        26093 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.009, whole genome shotgun sequence.
ACCESSION   DBJYSH010000009 DBJYSH010000000
VERSION     DBJYSH010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 26093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26093
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.009"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            35..553
                     /locus_tag="ACUBV3_000333"
     CDS             35..553
                     /locus_tag="ACUBV3_000333"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HHU3846869.1"
                     /translation="MDLNNYIASIENYPQEGITFRDISPLMADGKAYSYAVREIVQYA
                     ADKDIDMIVGPEARGFIVGCPVAYALGIGFAPVRKPGKLPREVISADYEKEYGLDTLT
                     MHADAIKPGQRVLIVDDLLATGGTVKATIEMIEKLGGVVAGCAFLVELDGLNGRKAIE
                     GYDTKVLMNFPG"
     gene            671..1351
                     /locus_tag="ACUBV3_000334"
     CDS             671..1351
                     /locus_tag="ACUBV3_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HHU3846870.1"
                     /translation="MTYLEQYQSGQLTLPSALFFHFKSIFKTADDFLVWQFFYLQNTT
                     NLSDLTPSRIATSLDKTVADINRSISNLTSQGLLDVKTIELNHEIEIIFDTSPVFAKL
                     DKLFEEDNQVIIDNKTADSNRLKDLVGDFERELGRLLSPFELEDLQKTLQEDQTDPDI
                     VRAALREAVFNGKTSWNYINAILRNWRREGLTTLRQIEERKQAREDNQMKDLAISDDF
                     KNAMNLWE"
     gene            1455..2138
                     /locus_tag="ACUBV3_000335"
     CDS             1455..2138
                     /locus_tag="ACUBV3_000335"
                     /EC_number="2.1.1.217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000365681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0160105 - tRNA
                     (adenine(22)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase"
                     /protein_id="HHU3846871.1"
                     /translation="MDLQLSKRLQKVANYVPKGARLLDVGSDHAYLPIFLLQMGYCDF
                     AIAGEVVNGPYQSALKNVSEHGLTSKIDVRLANGLSAFEEADNIDTIAICGMGGRLIA
                     DILNNDIDKLQHVKTLVLQPNNREDDLRKWLAANDFEIVAEDILTENDKCYEILVVKH
                     GHMNLTAKELRFGPFLLSNNTTVFKEKWQNELNKLTFALNSIPNSKMEERAILEDKIQ
                     DIKEVLDES"
     gene            2128..2916
                     /locus_tag="ACUBV3_000336"
     CDS             2128..2916
                     /locus_tag="ACUBV3_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HHU3846872.1"
                     /translation="MKARELIDVYETYCPQELSMEGDISGLQIGSLDKEIKTVMVALD
                     VRETTVAEAIERQVDLLIVKHAPIFRPLKDLVATPQNKIYIDLLKSDIAVYVSHTNID
                     IVPNGLNDWFCELLDIQYPDILSETSNGYGIGRIGDVRPQSFEFFAWKIKDVFGLDSV
                     RLVSYDKSNPEIQRVAICGGSGQSFYKEAIAKGADVFVTGDIYYHTAQEMITNGLLAI
                     DPGHHIEVLFVSKIATMIEQWKLEKGWDISVLESKAPTNPFYHM"
     gene            2925..4028
                     /locus_tag="ACUBV3_000337"
     CDS             2925..4028
                     /locus_tag="ACUBV3_000337"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HHU3846873.1"
                     /translation="MKKIAIIGAGAVGATLAYYLSKEKDIQVTVFDYGVGQATKAAAG
                     IISPWFSKRRNKAWYRMARLGADFYSKLVTDLQKDGFETKFYQQTGVFLLKKDESQLE
                     SLFALADKRRLESPLIGDLQILNKSEANTHFPELDGYEQLLYASGGARVEGADLTRIL
                     LEASGVNVIKDEVHFTITDNGFRVQGIDFDKLVLASGAWLAKILDEHNYQVDIRPQKG
                     QLRDYYFSNINTGKYPVVMPEGELDIIPFDNGKVSVGASHENDMAFDLNIDFKVLDKF
                     EEQAIGYFPQLKKADTTSERVGIRAYTSDFSPFFGPVPCMEGAYAASGLGSTGLTVGP
                     LIGYELCQLILNKENQLNLEDYDITKYVKKISR"
     gene            4087..4956
                     /gene="rfbA"
                     /locus_tag="ACUBV3_000338"
     CDS             4087..4956
                     /gene="rfbA"
                     /locus_tag="ACUBV3_000338"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002276675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HHU3846874.1"
                     /translation="MKGIILAGGSGTRLYPLTRAASKQLMPIYDKPMIYYPLSVLMLA
                     GIKEILIISTPQDLPRFEDMLGDGSELGISLSYAEQPSPDGLAQAFIIGEEFIGDDHV
                     ALVLGDNIYHGPGLSAMLQRAASKESGATVFGYQVKDPERFGVVEFDTDMNAISIEEK
                     PAQPKSNYAVTGLYFYDNDVVEIAKNIKPSPRGELEITDVNKAYLDRGDLSVELMGRG
                     FAWLDTGTHESLLEAAQYIETVQRMQNVQVANLEEIAYRMGYITREQVLELAQPLKKN
                     EYGQYLLRLIGEA"
     gene            4956..5549
                     /locus_tag="ACUBV3_000339"
     CDS             4956..5549
                     /locus_tag="ACUBV3_000339"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018379055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HHU3846875.1"
                     /translation="MTEQFFDKELTCRPIEAIPGLLEFDITVRGDNRGWFKENFQKEK
                     MIPLGFPESFFEADKLQNNISFNKKNTLRGLHAEPWDKYVSIADEGRVIGTWVDLREG
                     DSFGNVYQTIIDASKGIFVPRGVANGFQVLSDKAAYTYLVNDYWALELKPKYAFVNYA
                     DPNLGIQWENLEEAEVSEADKNHPLLKDVKPLKKEDL"
     gene            5756..6802
                     /gene="rfbB"
                     /locus_tag="ACUBV3_000340"
     CDS             5756..6802
                     /gene="rfbB"
                     /locus_tag="ACUBV3_000340"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HHU3846876.1"
                     /translation="MTEFKNIIVTGGAGFIGSNFVHYVYNNHPDVHVTVLDKLTYAGN
                     RANIEAILGDRVELVVGDIADAELVDKLAAKADAIVHYAAESHNDNSLEDPSPFIHTN
                     FIGTYTLLEAARKYDIRFHHVSTDEVYGDLPLREDLPGNGEGPGEKFTAETKYNPSSP
                     YSSTKAASDLIVKAWVRSFGVKATISNCSNNYGPYQHIEKFIPRQITNILAGIKPKLY
                     GEGKNVRDWIHTNDHSTGVWAILTKGRIGETYLIGADGEKNNKEVLELILEKMGQPKD
                     AYDHVTDRAGHDLRYAIDSTKLREELGWEPQFTNFSEGLEETIKWYTENETWWKAEKD
                     AVEAKYAKTQEVIK"
     gene            complement(6854..10072)
                     /gene="hylB"
                     /locus_tag="ACUBV3_000341"
     CDS             complement(6854..10072)
                     /gene="hylB"
                     /locus_tag="ACUBV3_000341"
                     /EC_number="4.2.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_192884539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronate lyase"
                     /protein_id="HHU3846877.1"
                     /translation="MEIKKKHRIMLYSALILGTILVNNSYQAKAEELTKTTSTSQIRD
                     TQTNNIEVLQTESTTVKETSTTTTQQDLSNPTASTATATATHSTMKQVVDNQTQNKEL
                     VKNGDFNQTNPVSGSWSHTSAREWSAWIDKGNTADKSPIIQRTEQGQVSLSSDKGFRG
                     AVTQKVNIDPTKKYEVKFDIETSNKAGQAFLRIMEKKDNNTRLWLSEMTSGTTNKHTL
                     TKIYNPKLNVSEVTLELYYEKGTGFVTFDNISMKAKGPKDSEHPQPVTTQIEKSVNTA
                     LNKNYVFNKADYQYTLTNPSLGKIVGGILYPNATGSTTVKISDKSGKIIKEVPLSVTA
                     STEDNFTKLLDKWNDVTIGNYVYDTNDSNMQKLNQKLDETNAKNIEAIKLDSNRTFLW
                     KDLDNLNNSAQLTATYRRLEDLAKQITNPHSTIYKNEKAIRTVKESLAWLHQNFYNVN
                     KDIEGSANWWDFEIGVPRSITGTLSLMNNYFTDAEIKTYTDPIEHFVPDAEYFRKTLV
                     NPFKALGGNLVDMGRVKIIEGLLRKDNTIIEKTSHSLKNLFTTATKAEGFYADGSYID
                     HTNVAYTGAYGNVLIDGLTQLLPIIQETDYKISNQELDMVYKWINQSFLPLIVKGELM
                     DMSRGRSISREAASSHAAAVEVLRGFLRLANMSNEERNLDLKSTIKTIITSNKFYNVF
                     NNLKSYSDIANMNKLLNDSTVATKPLKSNLSTFNSMDRLAYYNAKKDFGFALSLHSKR
                     TLNYEGMNDENTRGWYTGDGMFYIYNSDQSHYSNHFWPTVNPYKMAGTTEKDAKREDT
                     TKEFMSKHSKDAKEKTGQVTGTSDFVGSVKLNDHFALAAMDFTNWDRTLTAQKGWVIL
                     NDKIVFLGSNIKNTNGIGNVSTTIDQRKDDSKTPYTTYVNGKTIDLKQASSQQFTDTK
                     SVFLESKEPGRNIGYIFFKNSTIDIERKEQTGTWNSINRTSKNTSIVSNPFITISQKH
                     DNKGDSYGYMMVPNIDRTSFDKLANSKEVELLENSSKQQVIYDKNSQTWAVIKHDNQE
                     SLINNQFKMNKAGLYLVQKVGNDYQNVYYQPQTMTKTDQLAI"
     gene            10289..10771
                     /locus_tag="ACUBV3_000342"
     CDS             10289..10771
                     /locus_tag="ACUBV3_000342"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HHU3846878.1"
                     /translation="MTKLATICYIDNGKELLLLHRNKKENDVHEGKWISVGGKLEAGE
                     TPDECAKREILEETHLTVKKMDFKGVITFPEFTPGHDWYTYVFKVTDYEGELISDDES
                     REGTLEWVPYDQVLSKPTWQGDYEIFKWILEDVPFFSAKFVYDEHQNLIEKTVNFYEK
                     "
     gene            10761..11222
                     /locus_tag="ACUBV3_000343"
     CDS             10761..11222
                     /locus_tag="ACUBV3_000343"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HHU3846879.1"
                     /translation="MKNRDFRVRDQLETFGVRVSALIIENQKLLLIYAPHLYKYYLPG
                     GALQVGEDSNKAVAREVLEEIGLHSQVGDLAYIVENQFNIKRHHYHSVEFLYFVNLLG
                     QAPESIKEGTHKRHFVWLPIKELTKIDCNPNFLAQDLIEWPGHVVHKIVQN"
     gene            11314..12504
                     /locus_tag="ACUBV3_000344"
     CDS             11314..12504
                     /locus_tag="ACUBV3_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HHU3846880.1"
                     /translation="MNQPSEKEFKNSLFFKWILNNQAVIALMITFLVFLTIFIFTKIS
                     FMFKPVFDFLAVLILPLVISGLLYYLLKPMVTFLEKRGIKRVTAILSVFTIIILLLIW
                     AMSSFIPMMSNQLRHFMEDLPSYVNKVQMETSSFIDHNPWLKSYKGEISSMLSNISSQ
                     AVSYAEKFSKNVLDWAGNLASTVARVTVATIMAPFILFYLLRDSRNMKNGFLMVLPTK
                     LRQPADRILREMNSQMSGYVQGQIIVAITVGVIFSIMYSIIGLRYGVTLGIIAGVLNM
                     VPYLGSFVAQIPVFILALVAGPVMVVKVAIVFVIEQTLEGRFVSPLVLGNKLSIHPIT
                     IMFILLTSGAMFGVWGVFLSIPIYASIKVVVKELFDWYKAVSGLYTIDVVTEERSEEV
                     KNVE"
     gene            12494..13720
                     /locus_tag="ACUBV3_000345"
     CDS             12494..13720
                     /locus_tag="ACUBV3_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HHU3846881.1"
                     /translation="MLNSEKMIVSIQNQDLEHANKYFEKALKNDPEEILLELGAYLES
                     IGFLPQAKRLYDQIRPNYPEVAINLAQIVAEDGDIEQAFLYLDYISEDSQEYVSALLV
                     MADLYDMEGLTDVAREKLLLASKLSDDPLVTFGLAEMNLSLEHYQEAIEGYASLDNRE
                     ILETTGVSTYQRIGKSYAIIGKFDAAIEFLEKAVDIEYDDLTVFELATILYDQEEYQK
                     ANLYFKQLDTINPDFDGYEYIYGLSLREEHKSEEALRLVQQGLRKNSFDGQLLLLASQ
                     LSYELHDVPSSESYLKQAEKVSENQDEIVMRLSNLYLEEERFEEVLELDNDDLENILA
                     KWNIAKAHKALEMDDSVDYYQSLYNDLKDNPEFLQDYAYILREFGYLDKAQEVGKAYL
                     KLVPDDIEMSEWVNNI"
     gene            13830..15512
                     /gene="alsS"
                     /locus_tag="ACUBV3_000346"
     CDS             13830..15512
                     /gene="alsS"
                     /locus_tag="ACUBV3_000346"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0034077 - butanediol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HHU3846882.1"
                     /translation="MTESHNQYGADLIVDSLINHDVKYVFGIPGAKIDRVFDTLEDKG
                     PELIVARHEQNATFMAQAVGRITGEPGVVIATSGPGISNLATGLVTATDEGDAVLAIG
                     GQVKRGDLLKRAHQSMNNVAMLEPITKYSAEVHDPNTLSETVANAYRLAKSGKPGASF
                     ISIPQDVTDSPVSVKAIKPLSAPKLGSASVLDINYLAQAINNAVLPVLLLGNGASSEG
                     VTAAVRRLLDAVKLPVVETFQGAGIVSRELEDETFFGRVGLFRNQPGDMLLKRADLVI
                     AIGYDPIEYEARNWNAEISARIIVIDVEQAEIDTYFQPERELIGDMAHTLDLLLPAIK
                     GYELPEGSKEYLKGLRNNIENVSDVKFDRDSAHGLVHPLDLIDVLQDNTTDDMTVTVD
                     VGSHYIWMARYFKSYEARHLLFSNGMQTLGVALPWAISAALLRPNTKVISVSGDGGFL
                     FSAQELETAVRLHLPIVHIIWNDGKYNMVEFQEEMKYGRSSGVDFGPVDFVKYAESFG
                     AKGYRVDSKDSFEETLKQALIDAENGPVLIDVPIDYKDNVTLGETILPDEFY"
     gene            15526..16245
                     /gene="budA"
                     /locus_tag="ACUBV3_000347"
     CDS             15526..16245
                     /gene="budA"
                     /locus_tag="ACUBV3_000347"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HHU3846883.1"
                     /translation="MSETVKLFQYSTLSSLMAGLYKGSLTIGELLTHGDLGIGTVHMI
                     DGELIVLDGKAYQAIGTDGKAEIIQLSDDVTVPYAAVLPHHIQKQFDINAETDNKDLE
                     EMILKNFEGQNLFKSLKIKGTFSRMHVRMIPKSPQHKRFADIASNQPEFTRENVSGTL
                     VGIWTPELFHGVGVKGFHVHFISDDLTFGGHVMDYSLTQGKVEIGKVDQLDQCFPTQD
                     QEFLKANFDLQKLREDIDLSE"
     gene            complement(16299..17954)
                     /locus_tag="ACUBV3_000348"
     CDS             complement(16299..17954)
                     /locus_tag="ACUBV3_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990112 - RQC complex [Evidence IEA];
                     GO_function: GO:0001968 - fibronectin binding [Evidence
                     IEA];
                     GO_process: GO:0072344 - rescue of stalled ribosome
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rqc2 family fibronectin-binding protein"
                     /protein_id="HHU3846884.1"
                     /translation="MSFDGFFLHHLTNELQEQIEKGRIQKVNQPFDHELVLTIRNNRR
                     NYKLLLSAHPVFGRIQTTEANFQNPQNPNTFTMIMRKYLQGAVIETIQQIENDRILEI
                     IVSNKNEIGDHIKATLVVEIMGKHSNIILIDKNEHKIIESIKHVGFSQNSYRTILPGS
                     TYIAPPKTKAINPFDISDQTLFELLQTNDLSPKNLQQLFQGLGRDTALELSHCLKDNK
                     LNDFRQFFSREYYPSLTEKSFSAVQFSSSHETFQSLGQLLDYYYQEKAEKDRIAQQAS
                     DLIHRVQSELEKNIKKLAKQQDELLATENAEEFRQKGELLTTYLSMVPNNQDIVVLDN
                     YYTNQTIEISLDRALTPNQNAQRYFKKYQKLKEAVKHLKGIISDTKNTITYLESVETS
                     LNHASMEDINDIREELVETGFIKRRAHDKQHKRKKPEQYLASDGKTIIMVGRNNLQND
                     ELTFKMARKGELWFHAKDIPGSHVLIRDNLNPSDEVKTDAAELAAYYSKARLSNLVQV
                     DMIEAKKLNKPSGTKPGFVTYTGQKTLRVTPTQEKIDSLKLKK"
     gene            18313..19317
                     /gene="trpX"
                     /locus_tag="ACUBV3_000349"
     CDS             18313..19317
                     /gene="trpX"
                     /locus_tag="ACUBV3_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan ABC transporter substrate-binding
                     protein"
                     /protein_id="HHU3846885.1"
                     /translation="MKNKGLIATLILLTILVVGELFYNKSEKHLNLSEKQVVKIGILQ
                     YVTHDALDAIEKGVEDGLAQEGYKGKKVKLTVLNAEADQSKIQAMSKQLVNHHNDILI
                     GIATPSAQGLAASTKDTPIIMGAVSDPLGAKLVTNMKKPTTNVTGLSNVVPTKQTVQL
                     IKDITPNVKRIGILYASSEDNSVSQVTEFTKYAQKAGLEVLKYSVPSTNEIKTSMSVM
                     TKKVDAVFVPQDNTIASAFRTVIVAANQANIPVYSSVDTMVEQGSIASVAQSQYGLGL
                     ETAKQAIKVLRGKPVKDVPVKVIDTGKPSLNLKAAKHLGIKIPKKIMKQAEITVKVDD
                     "
     gene            19330..20193
                     /locus_tag="ACUBV3_000350"
     CDS             19330..20193
                     /locus_tag="ACUBV3_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HHU3846886.1"
                     /translation="MIISSVSQGLLWGILGLGIYLTFRILKFPDMTTEGSFPLGGAVC
                     VTLMNQGVNPILATILGMLSGMLAGFVTGLLYTKGKIPTILAGILVMTSCHSIMLMVM
                     KRANLGLNEIQTLKDFLPFSNDLNLLVLGLIAILLAISVLIYFLYTRLGQAYIATGDN
                     PDMAKSFGIDTDKMEMLGLIVSNGLIALSGALVSQQDGYADVSKGIGVIVIGLASIII
                     GEVLYSTGLTLFERLIAIVVGSILYQFLITAVIALGFNTNYLKLFSAIVLGICLMVPV
                     LKTKILKGVRL"
     gene            20193..20954
                     /locus_tag="ACUBV3_000351"
     CDS             20193..20954
                     /locus_tag="ACUBV3_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HHU3846887.1"
                     /translation="MMMKIIELKEATVQVSNGLAEMKTILDHVNLSIYEHDFITILGG
                     NGAGKSTLFNVIAGTLMLSSGNIYIMGQDVTNLSAEKRAKYLSRVFQDPKMGTAPRMT
                     VAENLLVAKFRGEKRPLVPRKITNYTEEFQKLIARTGNGLDRHLETPTGLLSGGQRQA
                     LSLLMATLKKPNLLLLDEHTAALDPRTSVSLMGLTDEFIKQDSLTALMITHHMEDALK
                     YGNRVLVMKDGKIVRDLNQAQKNKMAIADYYQLFD"
     gene            21251..22912
                     /locus_tag="ACUBV3_000352"
     CDS             21251..22912
                     /locus_tag="ACUBV3_000352"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0004534 - 5'-3' RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HHU3846888.1"
                     /translation="MSDIKIIALGGVRENGKNLYVVEVNDSIFVLDAGLKYPENEQLG
                     VDVVIPNLDYLIENKKRVQGIFLTHGHADAIGALPYIIAEVKAPVFGSPLTIELAKLF
                     VKNSTAVKKFNNFHVIDSETEIEFQDAVISFFKTTHSIPESMGIVIGTKEGNIVYTGD
                     FKFDQAARKYYQTDLARLAEIGRDGVLALLSDSANATSNEQVASEYEVGDEIKSVIED
                     AEGRVIVAAVASNLIRIQQVFDAAAENGRRVVLTGFDIENIVRTAIRMKRIHIADENM
                     IIKPKDMTRYEDNELLILETGRMGEPINGLQKMAIGRHRYVQIKDGDLVFIVTTPSIA
                     KEAVVARVENLIYKAGGSVKLITQNLRVSGHANGRELQLLMNLLKPKYLFPIQGEYRD
                     LSAHAGLAQEVGMSADDIYIVKRGDIMVLEKDGFFHSGSVPAGDVMIDGNAIGDVGNI
                     VLRDRKVLSEDGIFIVVITVSKKEKKIISKARVNTRGFVYVKKSRDILRESAELVNTT
                     VEDYLSKDTFDWGELKGKVRDEVSKFLFDQTKRRPAILPVVMEVR"
     gene            22969..23757
                     /locus_tag="ACUBV3_000353"
     CDS             22969..23757
                     /locus_tag="ACUBV3_000353"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HHU3846889.1"
                     /translation="MAFFNIEYHSKVLGTERQVNVIYPDAFEMSDDKIDDCDIPVLYL
                     LHGMGGNENSWQKRTNIERLLRHTNLIVVMPSTDLAWYTNTKYGLDYFDAIAIELPKV
                     LKRFFPNMSDKREKNFIAGLSMGGYGAYKIALLTNRFSHAASLSGALSFDFDLLFNNG
                     NDNTNYWSGIFGDLNNTDNIERHSLRRYVESFDMKTKFYAWCGYEDFLFEANEVAIDE
                     LRQLGLTIDYSNDHGKHEWYYWNQQLEKVLEWLPVDYVKEERLS"
     gene            23847..24557
                     /locus_tag="ACUBV3_000354"
     CDS             23847..24557
                     /locus_tag="ACUBV3_000354"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001259490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HHU3846890.1"
                     /translation="MRTLFRMIFAIPKFIFRLIWNIIWGIFKTVLVIAIILFGLYYYA
                     NHSQSEFANQLSDIIQTGKTFLNFADTNQLKNSFANLATDNVHHSENTRWDKNQATIY
                     IATRDSELASAYKQAISNWNATGAFVLQTTDNPNADIIAKDYSDAKTQAAGVAETERN
                     ALTNRISKVTVKLNTFYLKNKQFGYDHTRIVNTAEHELGHALGLGHNDQQHSVMQSKG
                     SHYGIQEVDIQTLKNLYA"
     gene            24795..25466
                     /gene="rpiA"
                     /locus_tag="ACUBV3_000355"
     CDS             24795..25466
                     /gene="rpiA"
                     /locus_tag="ACUBV3_000355"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HHU3846891.1"
                     /translation="MDELKKLAGVTAAKYVKNGMIVGLGTGSTAYFFVEEIGRRVKEE
                     GLQVIGVTTSNRTTEQARGLGIPLKSADDIDVIDVTVDGADEVDPDFNGIKGGGGALL
                     MEKIVATPTKEYIWVVDESKLVETLGAFKLPVEVVRYGSERLFRVFKSKGYCPSFRET
                     EGDRFITDMGNYIIDLDLKKIEDPKQLANELDHTVGVVEHGLFNGMVNKVIVASKNGL
                     DILEK"
     gene            25523..>26093
                     /locus_tag="ACUBV3_000356"
     CDS             25523..>26093
                     /locus_tag="ACUBV3_000356"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019770913.1"
                     /note="catalyzes the transfer of phosphate between the C1
                     and C5 carbons of pentose; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HHU3846892.1"
                     /translation="MSQFDRIHLVVLDSVGIGAAPDANDFVNAGVPDGASDTLGHISK
                     TVGLAVPNMAKIGLGNIPRPQALKTVPAEENPSGYATKLQEVSLGKDTMTGHWEIMGL
                     NITEPFDTFWNGFPEDIITKIEDFSGRKVIREANKPYSGTAVIDDFGPRQMETGELII
                     YTSADPVLQIAAHEDIIPLEELYRICEYAR"
BASE COUNT         8307 a         4062 c         5321 g         8403 t
ORIGIN      
        1 attttagggg tttcccctta gaaagacaag tgaaatggat ttaaataatt acatcgcttc
       61 aattgaaaac tatcctcaag aaggaattac tttccgtgat atctccccat taatggctga
      121 tggtaaagca tacagctatg ctgttcgtga aattgtacaa tatgctgctg ataaagatat
      181 tgatatgatt gttggaccag aagctcgtgg ctttattgtt ggttgcccag ttgcttatgc
      241 tcttggtatt ggttttgctc cagtccgtaa accaggtaaa ttaccacgcg aagttatttc
      301 agctgactat gaaaaagaat atggtcttga tacattgaca atgcacgcag atgctattaa
      361 accaggtcaa cgtgttttaa ttgttgatga ccttttagct actggtggaa cagtaaaagc
      421 gacaattgaa atgattgaaa aacttggtgg cgttgtcgca ggctgtgctt tcctagttga
      481 acttgatgga ttaaatggtc gtaaagctat tgaaggttat gatactaagg tattgatgaa
      541 ctttccagga taaggcctat tcaagataga tgaatgataa gggcgcctct aagtgctatg
      601 ataatagttg agaggtgctt tttgttacac atttcataat tttaggacga tagagaggat
      661 ttgaaaggtt atgacttatt tggaacaata tcagtcaggg caattgactc taccaagtgc
      721 tttatttttt cactttaaat ctatttttaa gacagcagac gattttttag tttggcaatt
      781 tttctattta caaaatacaa ctaatttatc cgatctaaca ccgagtcgta ttgcgacatc
      841 acttgataaa acagtggcag atatcaatcg ttctatatcg aatttaacaa gtcaaggttt
      901 actagatgtc aaaacaattg agttgaatca cgaaatcgag attatctttg atacaagccc
      961 tgtgtttgct aaattagata aacttttcga agaggataat caagtaataa tagacaataa
     1021 aaccgctgat tcgaatcgtc tgaaagattt agtaggagac ttcgaacgtg aattaggtcg
     1081 cttattaagt ccttttgaat tagaagattt acaaaagaca ctgcaagaag atcagacaga
     1141 tcctgatatt gttagggcag ctcttagaga agcggttttc aatgggaaaa caagttggaa
     1201 ttatatcaat gctatccttc gcaactggcg gcgtgaagga ttgacgacac ttcgacaaat
     1261 tgaagagaga aaacaagcac gtgaagataa ccaaatgaaa gatttagcga tttcggatga
     1321 ttttaaaaat gcaatgaatt tatgggaata agtttactag gaggagacag aatggttgat
     1381 acattaggtc tgctcctttt cttttgatgt taatgatgtt atacttttaa cagagaaaaa
     1441 ctgaaggaaa tacaatggat ttacaattat caaaacgatt acaaaaagtt gcaaactatg
     1501 ttccaaaagg agctcggtta ttagatgtag gaagtgatca tgcttactta cctatttttt
     1561 tgttgcagat gggatactgt gattttgcca ttgctggtga agtggtgaat ggaccgtatc
     1621 agtctgcgtt gaaaaacgtt tcagaacatg gtctcacttc aaagatagat gttcgcttag
     1681 caaatggttt gtcagctttt gaagaagcag ataatattga tacgatagct atctgtggaa
     1741 tgggtggtcg tttgattgct gatattttga ataacgatat agacaaactt cagcacgtta
     1801 aaacactagt cttacaacct aataatcggg aagatgattt aagaaaatgg ttagctgcaa
     1861 atgattttga aatagtagca gaagatatct tgactgaaaa tgacaaatgc tatgaaattt
     1921 tagttgttaa gcatggtcat atgaatttaa ctgctaaaga attgcgattt ggtccatttt
     1981 tattatccaa taacacaaca gtttttaaag aaaaatggca aaatgagctt aataaattaa
     2041 cttttgcttt aaactctatt cccaatagta aaatggaaga acgtgctatt ttggaagata
     2101 aaatacagga tataaaggag gttcttgatg aaagctagag aacttattga tgtatatgaa
     2161 acttattgtc cacaggaatt atctatggaa ggagatattt caggtcttca aattggatct
     2221 ttagataaag aaataaagac ggtcatggta gctttagatg ttcgagagac tacagtagct
     2281 gaagcaattg aacgtcaagt agatcttctt attgtaaagc atgctcctat ttttcgtcca
     2341 ttaaaggatt tggtagcaac accgcaaaat aagatttata tagaccttct taaatcagac
     2401 attgctgttt atgtgagcca tactaatata gatatcgttc ctaatggact taacgactgg
     2461 ttttgtgaat tattagatat tcaatatccc gatatattat ctgagacaag taatggttat
     2521 ggtattggtc gcattggtga tgttagaccc cagagttttg aattttttgc atggaaaatt
     2581 aaagatgtgt ttggtttaga tagcgttcgt ttagtttcat atgataaatc taatcctgaa
     2641 attcaacgag ttgctatttg tgggggtagt gggcaaagtt tctacaaaga agctattgca
     2701 aaaggagcag atgtttttgt aacgggagat atttattatc atactgcaca agaaatgatc
     2761 acaaatggtt tgcttgcgat tgacccaggc catcatattg aggttttatt tgtttctaaa
     2821 atagctacta tgattgagca gtggaaactt gaaaaaggct gggatatttc agttttagag
     2881 agtaaagctc caacaaatcc cttttaccat atgtaggagc tattatgaaa aagatagcaa
     2941 ttattggtgc aggagcagtc ggagcgacac tagcctatta cttatcaaaa gaaaaggata
     3001 ttcaggttac tgtttttgat tatggagttg gacaagcaac taaggctgct gcggggatta
     3061 ttagcccatg gttttcaaaa cgtcgtaata aagcctggta tcgcatggcg aggttggggg
     3121 ctgattttta ttcaaagcta gtcacggatt tacaaaaaga tggttttgaa acaaaatttt
     3181 atcagcaaac tggtgttttc ttattaaaaa aggacgagag tcagttagag tcattgtttg
     3241 cactagcaga taaacgtcga ctagaatctc ctctaatagg tgatttacaa atactaaata
     3301 aaagtgaagc aaatactcac tttcctgagt tagatggcta tgaacagtta ctttatgcga
     3361 gtggaggagc gcgagtagaa ggggcggact taacacgaat attgttagaa gcaagtggtg
     3421 ttaatgtcat caaggatgaa gttcacttta cgataactga taatggcttt cgtgttcaag
     3481 gaattgattt tgataaactt gttttagcga gtggagcctg gctagctaag atattagatg
     3541 agcacaacta tcaggtagat attcgccctc aaaaagggca attgcgagac tattatttta
     3601 gcaatattaa tacaggaaaa tatcctgttg taatgccaga aggcgagcta gatattatcc
     3661 catttgataa tggtaaagtc tctgtaggtg caagtcacga aaatgatatg gcttttgatt
     3721 taaacattga ttttaaagtc ttagacaagt ttgaagagca agctattggc tattttccgc
     3781 aattaaaaaa agcagatacg acttctgaac gtgttggaat tcgtgcctat acaagtgatt
     3841 tttctccatt ttttggccct gtcccttgta tggagggtgc atatgctgca agtggattag
     3901 gatcaactgg cttaacagtg ggcccattaa ttggttatga attatgtcaa ttaatcttaa
     3961 ataaagaaaa tcagctaaat ttagaggatt acgatattac gaaatacgtg aaaaagatta
     4021 gtagatgata gcgtttaaaa aaagtgttat aatatgtagg attaatataa ataaggagac
     4081 cttactatga aaggtattat tttagcggga ggttcaggta ctcgcttgta cccattaact
     4141 cgtgctgcat caaaacaatt gatgccaatt tatgataaac caatgattta ttatccattg
     4201 tcagttttga tgttggcagg aataaaagaa atactgatta tttctactcc acaagatttg
     4261 cctcgctttg aagatatgct tggagatggt tcagaactag gaatctctct ttcttatgct
     4321 gaacaaccaa gcccagatgg gttagcacag gccttcatta tcggtgaaga atttattggc
     4381 gatgatcacg ttgccttagt attaggtgat aatatttacc atggaccagg tttatctgca
     4441 atgctacaac gtgcagctag taaagaaagt ggagcaaccg tctttggcta tcaagtaaaa
     4501 gatccagaac gttttggtgt tgtcgagttt gatactgata tgaatgccat ttctattgaa
     4561 gaaaaaccag cgcaacctaa gtctaattat gctgtcacag gtctttattt ctatgataac
     4621 gatgtggttg aaatagctaa aaatattaag ccaagtcctc gtggagaact tgaaattact
     4681 gatgttaaca aagcctacct tgatagaggt gatttatcag ttgagttaat gggacgtgga
     4741 tttgcgtggt tagacactgg aactcacgaa agtcttcttg aagcagcaca atatattgaa
     4801 acagtacaac gcatgcaaaa cgttcaagtt gctaaccttg aggaaattgc ttaccgcatg
     4861 ggctacatca ctcgtgaaca agtccttgag ttggcacaac cacttaagaa aaatgagtat
     4921 ggtcaatacc tacttcgttt gattggagaa gcctaatgac tgaacaattt tttgataaag
     4981 aattaacttg tcgcccaatt gaagccattc ctggattgtt ggaattcgat attaccgttc
     5041 gtggagacaa ccgtggatgg ttcaaagaga actttcaaaa agaaaaaatg ataccgcttg
     5101 gtttcccaga aagcttcttt gaggcagaca aactacaaaa taatatttcg tttaacaaaa
     5161 aaaatacttt gcgaggtctc catgcagagc cttgggataa atatgtttcg atcgctgatg
     5221 aaggacgtgt gatcggtact tgggttgacc tccgtgaagg tgacagtttt ggtaacgttt
     5281 accaaacgat tatcgatgcc tcaaaaggta tttttgttcc acgcggcgtt gctaatggtt
     5341 tccaagttct ttcagataaa gcagcttata cttatctcgt taacgattat tgggcacttg
     5401 aactcaaacc aaaatatgct ttcgttaact atgcagatcc aaatctaggc attcagtggg
     5461 aaaaccttga agaagcagaa gtctcagaag cagataagaa tcacccactt ctcaaagatg
     5521 taaaaccttt gaagaaggaa gatttgtaat tttcaacttc ttaaaaggca ttaaccttag
     5581 caagtaaaaa cgaattagca atgttgtttg acgcaagggt gaaaggaaac atgcaaaagg
     5641 cctgttccct cagggatttg aaatattgat ttcttaggca tgacattcca tagaactact
     5701 tctaacagaa cagtatcctt caaacagcaa aaagaaaaaa tttttcggag aaaaaatgac
     5761 tgaatttaaa aacattatcg taactggtgg agctggtttc atcggatcaa actttgtaca
     5821 ctatgtttac aataaccacc cagatgttca tgtaactgtc cttgataaat tgacatatgc
     5881 aggtaaccgt gctaacattg aagccattct tggtgatcgt gttgagttag ttgttggtga
     5941 catcgctgac gctgaattgg tagacaaatt ggctgccaaa gcggatgcta ttgttcacta
     6001 tgcggctgag agtcacaacg ataactcatt ggaagatcca agtccattta tccatacaaa
     6061 ctttatcgga acttacactt tgcttgaagc agctcgtaaa tacgatatcc gtttccacca
     6121 cgtgtcaact gatgaagttt atggagatct tccacttcgt gaagaccttc caggaaatgg
     6181 tgaaggacca ggtgaaaaat tcactgctga aacaaaatac aatccatcat caccttactc
     6241 atcaactaag gcagcttctg accttatcgt taaggcatgg gtacgttcct tcggcgtaaa
     6301 agcgaccatt tcgaactgtt caaataacta tggaccatac caacacatcg aaaaattcat
     6361 tccacgtcaa attacaaata tcttggcagg aatcaaacca aaactttatg gtgaaggtaa
     6421 aaacgtccgt gactggatcc acacaaatga ccattcaaca ggtgtctggg ctattttgac
     6481 taagggtcgt attggtgaaa cataccttat tggtgccgac ggtgagaaaa acaacaagga
     6541 ggttcttgaa cttatccttg agaaaatggg tcaaccaaaa gacgcttacg atcacgtaac
     6601 tgaccgtgct ggtcatgatc ttcgttacgc tatcgattct acaaaattgc gtgaagaact
     6661 cggctgggaa ccacaattta caaacttttc agaaggtttg gaagaaacta ttaaatggta
     6721 tacagaaaat gagacatggt ggaaagcaga aaaagatgct gtagaagcca agtatgctaa
     6781 aactcaagaa gtcattaagt aattgaacca aaaaacctat cattttgatg ataggttttt
     6841 tatgtacggc tagttagata gctaattggt ctgtttttgt catggtttga ggttggtaat
     6901 agacattttg atagtcatta ccaacttttt gtactaggta aagtcccgct ttattcattt
     6961 tgaattgatt gttaatgaga ctctcttgat tatcgtgttt gataacagcc caagtttgac
     7021 tgtttttatc atagataact tgttgttttg aactattttc tagtaattct acttctttgc
     7081 tgttggctaa tttatcaaaa cttgtgcgat caatgtttgg aaccatcatg taaccatagc
     7141 tatcaccttt gttgtcatgc ttttggctta tagtgataaa aggattgcta acgattgagg
     7201 tatttttaga agtacgatta atgctgttcc aagtacctgt ttgctctttg cgttcaatat
     7261 caatagtgct atttttaaag aagatataac caatattgcg accaggttct tttgattcta
     7321 aaaagacact ttttgtatct gtaaattgtt gagaacttgc ttgttttaaa tcaatagttt
     7381 ttccattgac gtatgtagta taaggtgttt tagaatcgtc ttttcgttga tcaattgttg
     7441 tagaaacatt tccaatgccg ttagtattct tgatgttgct acctaaaaag acaatcttat
     7501 catttaagat aacccaacct ttttgtgctg ttaaggtgcg atcccagtta gtaaaatcca
     7561 tagcggcaag agcaaagtga tcattaagtt tgacggaacc aacaaagtca gatgttcctg
     7621 taacttgacc ggttttttct ttagcgtctt tgctatgttt gctcatgaat tccttagtgg
     7681 tatcttcacg cttagcatct ttttcagttg ttccagccat tttataagga ttgacggttg
     7741 gccaaaaatg attactataa tgagattgat cactattata aatatagaac ataccatctc
     7801 cggtatacca accacgtgta ttttcatcat tcattccttc atagttgagg gtacgtttag
     7861 aatgtaatga aagcgcgaaa ccaaagtctt tcttggcatt ataataagct aagcggtcca
     7921 tgctattaaa ggttgataaa ttacttttta aaggtttagt agcgactgta ctgtcattaa
     7981 gcagcttatt catgttggca atatcggaat acgatttgag gttattgaag acattgtaga
     8041 atttatttga agtgataatc gttttaatag ttgatttgag gtctaagttt cgctcttcat
     8101 tagacatgtt agccaatctg aggaaacctc tgagaacttc aactgcagcc gcatgcgaag
     8161 aagctgcctc tctactaatt gagcgtccac gactcatatc cattaactca ccttttacaa
     8221 ttaaaggtaa aaatgattga ttaatccatt tataaaccat atcaagttct tgattagaga
     8281 ttttatagtc agtttcttga atgataggca gcaattgtgt caaaccatct atcagaacat
     8341 taccataggc gccagtataa gcaacatttg tatggtcgat gtaagaaccg tcagcataga
     8401 agccttcagc tttagtagca gtagtaaaaa gattttttag agaatgagaa gttttttcga
     8461 taatagtatt gtctttacga agtaaacctt caatgatttt aacgcgcccc atatctacta
     8521 gattaccacc aagggcttta aatggattga caagcgtttt acggaaatat tctgcatcag
     8581 gaacaaagtg ttcaattggg tcggtataag tttttatttc agcgtcagtg aagtagttat
     8641 tcatgagaga tagggtacct gtaattgagc gaggaacacc gatttcaaaa tcccaccaat
     8701 tggcagagcc ttctatatct ttattaacat tgtagaagtt ttgatgaagc caagccagac
     8761 tctcttttac agtacgaata gctttttcat ttttgtaaat agtagagtgt ggattggtga
     8821 tttgtttagc taaatcttcc aaacgacgat aagtagcggt taactgtgct gaattattga
     8881 gattatctaa atctttccaa aggaaagtgc gattagaatc cagtttgata gcttcgatgt
     8941 ttttggcgtt agtttcatct aatttctgat taagcttttg catgttcgaa tcattagtat
     9001 cgtaaacata attaccaata gtcacgtcgt tccatttgtc gaggagtttt gtaaagttat
     9061 cttctgttga agctgtaact gataacggta cttctttaat tattttgcca gatttatcag
     9121 atattttaac agttgttgaa ccagtagcgt ttggatacaa tattccacca acaattttcc
     9181 caagagacgg attggttaga gtgtattggt agtcagcttt attaaaaacg taatttttgt
     9241 ttaaagccgt attaacgctt ttttcaattt gtgttgtgac gggttgtgga tgctctgagt
     9301 ctttagggcc ttttgctttc attgatatat tatcaaaagt aacaaaacca gttccttttt
     9361 cataataaag ttcaagtgtc acctcggaga catttaactt tgggttatat atctttgtta
     9421 aggtatgttt gttagtagta ccgctggtca tctcagaaag ccaaagtcgc gtattgttgt
     9481 cttttttctc cataatacga aggaaagctt gtccagcctt gttacttgtt tcaatatcaa
     9541 acttaacctc atatttttta gtgggatcaa tgttcacttt ttgtgttaca gcacctctaa
     9601 agcctttgtc gctggataga cttacttggc cttgttcggt acgttggata ataggtgatt
     9661 tatcagcagt atttccttta tcaatccaag cagaccattc cctagcgctt gtatgtgacc
     9721 agcttccaga tacagggtta gtttgattaa aatctccgtt tttcaccagc tccttatttt
     9781 gagtttgatt atctactact tgtttcattg tgctatgagt ggctgttgca gttgcagttg
     9841 aagctgtggg gttagacaga tcttgttgtg tggttgtggt gctagtctcc ttgacagtgg
     9901 tactttcagt ctggagaact tcaatattat tagtttgagt atctcttatt tgggacgttg
     9961 aggtagtttt ggtaagctct tcagctttag cttggtaact attgttaacc aatattgttc
    10021 ctaaaataag ggctgaataa agcataatac gatgtttctt tttgatttcc atttttactc
    10081 cttagggttt aaaattgtaa accggttttt cctaattata gcatgcttta aaggtttatg
    10141 ataaagaacc tttaaaatgt catcaaagtg aaaagtaaat aactggtaaa caaaataatt
    10201 tgtttaatca tttgtgtgct actttgatag taaattgcct aaataaaggc tattttttgt
    10261 tataatatga gaatgagagg agtttgaaat gactaaatta gcaacaattt gttacattga
    10321 caatggcaag gaattacttc tattgcatcg caacaaaaaa gagaatgatg ttcatgaggg
    10381 aaagtggatc tcggtaggcg gtaaactgga ggcaggtgaa actccggatg aatgtgctaa
    10441 gcgtgaaatt ctagaagaaa cccatttaac agtaaaaaaa atggatttta aaggagtcat
    10501 cacttttcct gaatttacgc caggtcatga ttggtatacc tatgtcttta aagtaacaga
    10561 ttatgaagga gagttaattt cagatgatga atcacgagaa ggaaccttag aatgggtacc
    10621 atatgatcaa gttctctcta agccaacgtg gcaaggagat tacgaaattt ttaaatggat
    10681 tttagaagat gttcctttct tttctgctaa gtttgtttat gatgaacacc aaaatttaat
    10741 tgaaaagacg gttaattttt atgaaaaata gagattttag ggttagagac cagctcgaaa
    10801 cattcggagt cagagtttct gctttaatca tagaaaatca aaaactgcta ttaatatatg
    10861 ctcctcatct ctataaatac tatttgccag gtggggcgct tcaagttgga gaagatagca
    10921 ataaggctgt tgcgagagaa gtacttgagg aaataggtct tcacagccaa gttggtgatt
    10981 tagcctatat tgttgagaat cagtttaata ttaagagaca tcattatcac tcagttgaat
    11041 ttttatattt tgttaatctc ttgggtcagg cgccagaatc tatcaaagaa ggaacgcata
    11101 agcgacattt tgtatggctt ccaataaaag aattaacaaa aattgattgt aatcctaatt
    11161 ttttagcaca ggatttgata gaatggccag gtcatgttgt gcataaaatc gttcaaaatt
    11221 aactttttac tctcttgttt tgagtttaat ttaatttaac gtgttataat aaatgagaat
    11281 taaaattaga attcatcttg aaaggatatg tgaatgaatc aaccatcaga aaaagaattt
    11341 aaaaatagtt tgttttttaa atggatctta aataatcaag cagttattgc tctcatgatt
    11401 acctttttgg tatttttaac gatttttatt tttaccaaaa tctcttttat gtttaaacct
    11461 gtgtttgatt ttcttgctgt gctgatattg ccgcttgtaa tttctggctt gctttattac
    11521 ctattaaaac ctatggttac atttttagag aagcggggaa ttaagcgtgt aacagcgata
    11581 ttatcagttt ttactattat aatccttctg ttaatttggg caatgtctag ttttattccc
    11641 atgatgagta atcaattacg ccattttatg gaagatctcc cttcatatgt gaataaagtg
    11701 caaatggaaa caagttcgtt tatagatcac aacccttggt taaaatctta taaaggggaa
    11761 atatcgagca tgttatctaa tatcagtagc caagcggtct cttatgctga aaaattttca
    11821 aagaatgttt tagattgggc aggaaattta gctagtacag ttgcacgtgt gacagtagca
    11881 acaatcatgg ctccctttat tttgttttat cttttaagag atagtcgcaa catgaagaat
    11941 ggtttcttaa tggttttacc aaccaaacta cgccaaccag ctgatcgtat tttgcgagaa
    12001 atgaatagtc aaatgtcagg atatgtgcaa ggacaaatca ttgttgctat tactgttggt
    12061 gttatttttt caataatgta tagtattata ggccttagat atggcgtgac attagggatt
    12121 attgccggtg tgttaaatat ggttccctat ttgggaagtt ttgtcgccca aattccagtg
    12181 tttatcttag cgcttgtcgc aggacctgtt atggttgtta aagttgcgat tgtttttgtt
    12241 attgagcaaa ctctagaggg acgctttgtc tcacctttgg ttttaggtaa taaacttagc
    12301 attcatccaa ttacaattat gtttatttta ttaacctctg gagcgatgtt tggtgtttgg
    12361 ggagtattcc tcagtattcc gatttatgca tctatcaaag ttgttgttaa agaattgttt
    12421 gattggtaca aagctgtcag tgggctatat acaatagatg ttgttactga agaaagaagt
    12481 gaagaagtta aaaatgttga atagtgaaaa aatgattgtt tctatccaaa atcaggattt
    12541 agaacatgct aacaagtatt tcgaaaaagc gttgaaaaat gatcctgagg aaatcttatt
    12601 ggaactagga gcttatttgg agagtattgg ttttttaccc caagcaaaac gtctctatga
    12661 tcaaatccga ccaaattatc cagaagttgc tatcaattta gctcaaattg ttgccgaaga
    12721 tggagatatt gaacaagcat tcttatactt agattatatc tcagaagata gtcaagagta
    12781 tgtttcagcc ctcctcgtta tggctgactt atatgatatg gaaggcttga ctgatgttgc
    12841 gcgtgaaaag cttttgctag ccagcaaact tagcgatgac ccattggtta catttggttt
    12901 agctgaaatg aaccttagtt tggaacatta tcaagaagcg atagaaggat atgcatcact
    12961 tgacaatcga gaaatcttag aaacgactgg agtttcaact tatcagcgta ttggtaaatc
    13021 ctacgctatt atagggaaat ttgatgcagc tatcgaattt ttagaaaaag ccgttgacat
    13081 tgagtatgat gatttaacag tttttgagtt agcaacaatc ctttatgatc aagaagaata
    13141 tcaaaaagct aacctttatt ttaaacagtt ggatactatt aatccagact ttgatggcta
    13201 tgaatatatc tatggtttat ctcttcgtga ggagcataaa tctgaagaag ctttacgtct
    13261 agttcagcaa ggtcttcgta aaaatagttt tgatggtcaa cttctcttac tagcttcaca
    13321 attatcgtat gaattacatg atgttccttc gtcggagtct tatttgaaac aagctgaaaa
    13381 agtttctgaa aaccaagatg aaattgttat gagattgtca aatttgtatc ttgaagaaga
    13441 gcggtttgag gaagtcttgg aattagacaa tgatgattta gaaaatatcc ttgcaaaatg
    13501 gaatatcgct aaagcacaca aggctttaga aatggatgat agtgtagact attatcaatc
    13561 tttgtataat gatttaaaag ataatccaga atttttacaa gattatgctt acattttgag
    13621 agaatttggt taccttgata aggcacaaga agtaggaaag gcttatttaa aattagttcc
    13681 agacgatata gagatgtcag aatgggtgaa taacatttaa ataggaaaac acttacagaa
    13741 aaaaataaaa aattcacaag taaattttca caatctgatg taaaaactgt gatataattg
    13801 aaccattctt gtgaaaagag ggagataaaa tgacagaatc acataatcaa tatggggctg
    13861 acttaattgt cgatagcctc attaatcatg atgtcaaata tgtttttggt atcccaggag
    13921 ctaagattga ccgcgtattt gacactctag aagataaagg tccagagtta attgttgctc
    13981 gtcatgagca gaacgcgact tttatggctc aggctgtcgg gcgtattacg ggagaaccag
    14041 gagttgttat tgcaactagt ggacctggga tctcgaactt ggcaacaggg ttagttaccg
    14101 cgacagatga aggtgatgct gttcttgcaa ttggtggaca ggttaagcga ggagatttat
    14161 tgaaacgtgc tcatcaatca atgaataacg ttgctatgct agaacctatt acaaaatatt
    14221 ctgctgaggt tcatgaccct aatactttgt cagaaactgt tgcaaacgcc tatcgtcttg
    14281 ctaaatctgg taaaccagga gcaagtttta tctctattcc tcaagacgtt acagacagtc
    14341 cagtctcagt aaaagcaatc aaacctctta gtgcacctaa attgggttcg gcatctgttt
    14401 tagatattaa ttatttagcg caagctatta acaatgctgt tttaccggta ctgttattag
    14461 gaaatggcgc atcttcagag ggagtaactg ctgccgtacg tcgacttctt gatgctgtta
    14521 aactaccagt agtagaaact ttccaaggtg cgggtattgt gtcacgtgag ctcgaggatg
    14581 aaacattctt tggtcgtgta ggcctcttcc gtaatcaacc tggagatatg cttttgaagc
    14641 gtgcagacct agttattgct atcgggtatg accctattga atatgaggcg cgtaactgga
    14701 atgctgaaat ttctgctcgt attatcgtta ttgacgttga acaagcagaa attgatacgt
    14761 atttccaacc cgagcgagaa ctgattgggg acatggcaca tacacttgat ttacttttac
    14821 cagctattaa aggatatgaa ttgccagaag gttcaaaaga gtatttgaaa ggtcttcgta
    14881 ataacattga aaacgttagc gatgttaaat ttgatcgtga ctcagctcat ggacttgttc
    14941 atcctcttga tctcattgat gtactccaag ataataccac cgatgacatg actgttacag
    15001 ttgatgtggg aagccattat atttggatgg ctcgctactt taaatcatat gaagcgcgtc
    15061 acttgctctt ctcaaacgga atgcaaacat taggagttgc tctaccttgg gcaatttctg
    15121 cggctctttt gcgtcccaac acaaaagtta tttcagtttc tggtgatggt ggcttcttat
    15181 tctcagctca agagttagaa actgcagttc gtcttcatct accaattgtt cacattattt
    15241 ggaacgatgg aaaatataac atggttgaat tccaggaaga aatgaagtat gggcgttctt
    15301 caggtgttga ctttggacca gttgattttg taaaatacgc tgaaagtttc ggtgctaaag
    15361 gttaccgtgt agacagcaaa gacagttttg aagaaacact caaacaagct ctaatcgatg
    15421 ctgaaaatgg accagtgttg attgatgtgc ctattgacta taaagataac gtgacactag
    15481 gtgaaacaat cttacctgac gagttctact aagacgaggt ctaatatgtc agaaacagtt
    15541 aaattatttc aatacagcac cttgagttca ctgatggcag gattgtacaa aggcagtcta
    15601 actatcggag aattattaac tcacggtgac ttaggaatcg gtactgttca tatgattgat
    15661 ggtgaattga ttgtattgga tgggaaagct tatcaagcta ttggtacaga tggtaaagca
    15721 gagattatcc aattatcaga tgatgtgaca gtaccctatg cagcggtttt accacaccat
    15781 atccaaaaac aatttgatat caatgctgag acagataata aggatttgga agagatgatt
    15841 ctaaaaaact ttgaaggtca aaatctcttt aagtctttaa aaatcaaagg aacgttttca
    15901 agaatgcacg ttcgtatgat tccaaaatct ccacaacata aacgttttgc agatattgca
    15961 tctaatcagc cagaatttac cagagagaat gtatctggta cgttggttgg gatttggacg
    16021 ccggaattat ttcatggtgt gggggtaaaa gggttccatg tccactttat ttcagacgat
    16081 ttaactttcg gtgggcatgt tatggattac tcgctcacac aaggaaaagt tgaaattgga
    16141 aaggttgacc aacttgacca atgtttccca actcaagatc aagaattttt gaaagctaac
    16201 tttgacctac aaaagcttag agaagatatt gatttaagtg aataagaata cctataaata
    16261 attaataaca ctaaaaatag acagggatat gtcccagtct atttttttag tttaaggcta
    16321 tctatttttt cttgtgtggg agtgactcgt aaggtttttt gaccagtgta agttacaaaa
    16381 cctggcttag taccacttgg tttattgagt ttttttgctt caatcatgtc tacttgaaca
    16441 agattggaga gacgagcttt agagtaataa gcagctaact cggcggcatc tgtcttaacc
    16501 tcgtcacttg gattgaggtt atcccgaatt aacacatggc ttccggggat atccttggca
    16561 tgaaaccaca actccccttt acgagccatt ttaaatgtta actcgtcatt ttggagatta
    16621 ttacgtccca ccataataat agtcttgcca tcggatgcta aatattgttc aggtttttta
    16681 cgcttatgtt gtttatcatg tgcgcgtcgc ttaataaatc cagtttcaac aagttcttca
    16741 cgaatatcat tgatatcttc catagaagca tgatttagcg atgtttcaac agattcaagg
    16801 taggtgattg tatttttcgt atctgaaata attcctttta aatgttttac agcttctttt
    16861 aatttttgat attttttaaa gtagcgttgg gcattttggt tgggtgttaa agctcgatca
    16921 agtgaaattt caatcgtttg gttggtgtaa taattatcaa gaacaacgat atcttgatta
    16981 tttggcacca tagagagata ggtagttaga agctctcctt tttggcgaaa ctcctctgca
    17041 ttttcagtag ctagaagttc gtcttgttgc ttagctagtt ttttgatatt tttctctaac
    17101 tcactttgaa cacggtgaat gaggtcacta gcttgctgtg ctatgcgatc tttttcagcc
    17161 ttctcttggt agtaataatc taacaattgt ccaagagact gaaatgtttc gtgactgctt
    17221 gagaattgga cagcggaaaa cgatttttct gttaggctag gataatattc tctcgaaaag
    17281 aattgacgaa aatcgttaag tttattgtct ttcaaacagt gagataattc tagcgcagta
    17341 tcgcgtccta aaccttggaa gagctgttgg aggtttttag ggcttaggtc attggtttga
    17401 agcagctcaa atagagtttg atcagaaata tcaaaaggat tgattgcttt tgttttgggt
    17461 ggggcaatgt aagttgaacc aggaaggata gtacggtagg agttttgcga gaaaccaaca
    17521 tgcttaattg attcaataat tttatgttca tttttatcaa tcaagataat gttactgtgt
    17581 tttcccatga tttcaacaac aagtgtcgct ttaatgtgat caccgatttc gtttttatta
    17641 gacacgatga tttctaatat acggtcattt tcaatttgtt gaatggtttc aatcactgca
    17701 ccttgcaaat actttctcat gatcattgta aaagtatttg gattttgagg attttggaaa
    17761 tttgcttcgg tagtttggat acgtccaaat acagggtgag cagataggag tagtttatag
    17821 ttgcgacgat tattgcgtat tgttaatacc aattcatggt caaaaggttg gttaactttt
    17881 tggatacgtc ctttttcaat ctgttcttgt aattcatttg tgaggtgatg taaaaaaaat
    17941 ccatcaaaag acatggcgtc ccccttattc taaaaattat agcttattta ttataccata
    18001 ggctgagcta aaaatatttt tttataacgc aaaaggcttg caattttcag aatatagtat
    18061 ataattatag aaaaattcat gagaaaggat gactagtaaa tgactaaaac attatttacc
    18121 aattggcaaa actggcgtag ataaagttgt gtttatgggt gcatagatac gactttttgg
    18181 agtacttcaa aaaagtatct atgcgctggt ctagtctaac aatagagagc gcaaggtaag
    18241 ttatttatca ggcgcttttt cttttgtgct aaactccaga aagtcaggat attattaaag
    18301 tgaggagaaa atatgaaaaa taaaggactg atagctacgc taattctatt gaccatatta
    18361 gtggttgggg aattatttta taacaaatca gaaaaacatc ttaatttatc agaaaagcaa
    18421 gttgttaaaa ttggtatttt acagtatgta acccatgatg ctttggatgc tattgaaaag
    18481 ggagttgaag atggtctagc tcaagaagga tataaaggga aaaaagttaa attaaccgtt
    18541 ttaaacgccg aagctgatca aagtaaaatt caagcgatga gtaaacaatt agttaatcat
    18601 cacaatgata ttttgattgg aattgcgaca ccatcagcac aaggtttagc agcttcgact
    18661 aaggatactc ctataatcat gggagctgtt tctgatcctt taggggccaa gttagtaacg
    18721 aatatgaaga aaccaacaac taatgttaca ggattatcaa acgttgttcc gaccaagcaa
    18781 actgtgcaat taatcaagga tataactccc aatgttaaac gtataggaat tctttacgct
    18841 agtagcgaag ataattctgt gtcacaagta actgagttta caaaatatgc tcaaaaagca
    18901 gggctagaag ttcttaaata ttctgtacca tctacaaatg aaattaaaac aagtatgtca
    18961 gtcatgacca aaaaagtaga cgctgtcttt gttccacaag ataatactat tgcgagtgct
    19021 tttagaacag ttatcgtagc agcaaaccaa gctaatatac ctgtgtattc cagtgttgat
    19081 accatggttg aacaaggaag tatagcatca gtagcgcaga gtcaatatgg cttagggtta
    19141 gaaactgcaa aacaggccat taaagtccta agagggaagc cggttaaaga tgtaccagtc
    19201 aaagttattg atacagggaa gccaagcctc aatttgaaag cagccaaaca cttaggaatt
    19261 aaaattccta agaagattat gaaacaggca gaaattaccg ttaaggttga cgattagaaa
    19321 gagggtttta tgattatttc atcagtttca caaggtttat tatgggggat attaggatta
    19381 gggatatact taacctttag aatcttaaaa tttccagaca tgacaacaga gggctccttc
    19441 cctctagggg gagcggtttg tgttacctta atgaatcaag gggttaatcc tattttggcg
    19501 acgattctag gcatgctatc agggatgtta gcgggatttg ttactggttt actttatact
    19561 aaaggaaaga taccgacgat tttagcaggg attttagtaa tgacctcatg ccactctatt
    19621 atgctaatgg ttatgaaaag agcaaatcta ggtttaaatg agattcaaac attgaaagat
    19681 tttcttccgt tttcaaatga tttaaatctt ctcgtattag gtttgatagc tattctcttg
    19741 gcgatttctg tcttaattta ttttctctat acacgcttgg ggcaagctta tattgcaaca
    19801 ggagacaacc ctgatatggc aaagagcttc ggcattgata ccgacaaaat ggaaatgctt
    19861 ggcttaattg tatcaaatgg cttaattgct ttatcaggag ctttagtaag tcaacaagat
    19921 ggatatgctg atgtatcaaa aggtataggt gttattgtta ttgggttagc tagtattatt
    19981 attggagagg tactttactc tactggttta actctctttg aacgtttaat tgctatagtg
    20041 gtgggttcta ttctttatca atttttaata acggcagtaa tcgccttagg atttaatacg
    20101 aattacctaa aattatttag tgcaatagtg cttggaattt gtttaatggt tcctgtccta
    20161 aaaacgaaga ttttgaaagg agtacgctta taatgatgat gaaaatcata gagttaaaag
    20221 aagcaaccgt acaagtcagt aatggtttag cagaaatgaa aacgatatta gatcatgtta
    20281 atttgagtat ttatgaacat gacttcatta caatattggg tggaaatgga gctggaaaat
    20341 caacgctttt taatgtaatt gcaggtacct tgatgttaag cagtggaaat atttacatca
    20401 tgggacaaga tgttactaat ttatcagcag aaaaacgagc taaatattta tcacgggtat
    20461 ttcaagatcc gaaaatggga acagcaccta ggatgacagt tgctgaaaat ttattagttg
    20521 ctaaatttcg aggtgaaaag agaccgttag ttcctagaaa gattactaac tataccgaag
    20581 aatttcaaaa attgattgct agaactggta atggacttga tcgccattta gagacaccta
    20641 caggtttatt atcaggtgga caaagacaag cgcttagttt attaatggca actttgaaaa
    20701 aaccaaatct gttattattg gacgaacaca cagcagcgct tgatcctaga acaagtgttt
    20761 cgcttatggg cttgactgat gagtttatca aacaagattc gttgactgcc ttaatgatta
    20821 cacatcatat ggaagatgct ctcaaatatg gaaatcgtgt attagtaatg aaagatggca
    20881 agattgtcag agatttaaat caggcacaaa aaaacaagat ggctatagct gactattatc
    20941 aattatttga ttaaaaagaa gtgaaatgga agtgtttcac ttctttttct atacactttg
    21001 caagaaatta ttttcaaaat atgatatact aataatgctg taaatcgact tgtagagaca
    21061 aggaccgtaa gtacaaatgg aatgaaaata aggacacact ctttaattca tattcctatt
    21121 attgattaat aaggtttttc ttcaaaccca agaaaagtta ccgataaatc aattactaat
    21181 gataataatt ggacaatttc ttgtctgctt tataaaaaag aattaacaaa atttaaggag
    21241 aaaactcatt atgagcgaca taaaaatcat tgctcttgga ggtgttcgtg aaaacggaaa
    21301 aaacctatat gtagtagaag tcaatgattc tatctttgtt ttggatgctg gactcaaata
    21361 tcctgaaaat gaacaactgg gtgttgatgt tgttattcca aatcttgatt acttaattga
    21421 aaataaaaaa cgtgttcagg ggattttctt aacccatgga catgcggatg ctattggtgc
    21481 attgccttat attatcgccg aagtcaaagc acctgtgttt ggttcacctc tcacaattga
    21541 attagctaag ttattcgtta aaaatagtac agcagtcaag aaattcaata atttccatgt
    21601 gattgatagt gaaacggaaa ttgaatttca agatgcggtg atttcgttct ttaaaacgac
    21661 tcattcaatt ccagagtcaa tgggaattgt tattgggaca aaagagggaa atatcgttta
    21721 cacaggtgac tttaaatttg atcaagctgc aaggaaatat taccaaacag atctggcgcg
    21781 tttagctgaa attggtcgtg atggtgtttt agcactttta tcagattctg caaatgctac
    21841 aagcaatgag caagttgcta gcgaatatga ggtcggtgat gaaattaaga gcgttattga
    21901 agacgcagaa ggtagagtca tcgtagcggc agttgcaagt aatctcattc gtattcaaca
    21961 agtctttgat gcagcggcag aaaacggtcg tcgagtagtt ttaacgggtt ttgatattga
    22021 aaatattgtt cgtacggcca ttcgtatgaa acgcattcat attgctgatg aaaatatgat
    22081 tatcaaacct aaagatatga cacgttacga agataacgaa ttattaatcc ttgaaactgg
    22141 acgtatggga gagccgataa atggtctcca aaaaatggcg atcggacgtc atcgttatgt
    22201 tcaaattaaa gatggtgatt tagtatttat cgtaacaaca ccaagtattg caaaagaggc
    22261 agttgtagcg cgtgttgaaa atctgattta taaagctggt ggctcagtta aattgataac
    22321 acagaatttg cgcgtatcag gtcatgcaaa tggtcgcgaa ttacaattat taatgaatct
    22381 attgaaacct aaataccttt tcccaattca aggagagtat agggatttgt cagcacatgc
    22441 gggcttagct caagaggtag gaatgtcagc agatgatatt tatattgtga agcgtggtga
    22501 catcatggtt cttgaaaaag atggcttctt ccatagtgga tctgttcctg ctggagatgt
    22561 tatgattgat ggaaatgcta ttggtgacgt tggtaatatt gtacttcgtg accgcaaggt
    22621 attatctgaa gatggaattt ttattgttgt tataactgtc agcaaaaaag aaaagaaaat
    22681 tatttctaaa gctcgtgtta atacgcgtgg ttttgtgtat gtgaaaaaaa gcagagatat
    22741 tttgcgtgag agcgcagaat tagttaatac aacggtagaa gattacttaa gtaaagatac
    22801 atttgactgg ggtgaactta aagggaaagt gcgtgatgag gtcagcaagt tcttatttga
    22861 ccaaacgaaa cgtcgcccag caatccttcc tgtggttatg gaagttcgtt gatagtgtta
    22921 agaggttacc aaattttggt aacctttctt gctagaatgg aggtttttat ggcatttttt
    22981 aatattgaat atcactctaa ggtactagga acggaacgtc aagtcaatgt tatttaccca
    23041 gatgcttttg agatgtctga tgataaaatt gatgattgtg acattcctgt tttgtatctt
    23101 ctccatggaa tggggggaaa tgagaattct tggcaaaaac gcacaaatat agagcgtctt
    23161 ttacgtcata ctaatcttat cgtcgtgatg ccatcaacag acttagcatg gtataccaac
    23221 acaaagtatg ggttggatta ttttgatgca atagctattg agttaccaaa agttttgaaa
    23281 cgtttctttc ctaatatgtc tgataagaga gagaaaaatt tcattgccgg attatctatg
    23341 gggggatatg gagcatataa aatagctcta ctgactaatc gtttttctca tgctgcctct
    23401 ttgtcaggtg cactttcttt tgactttgat ttattattca ataatggcaa cgataacact
    23461 aactattggt cgggaatttt tggtgatttg aataacactg ataacataga gcgtcattca
    23521 ctaaggcgtt acgttgagtc ttttgatatg aagactaaat tttatgcatg gtgtggttat
    23581 gaagattttc tttttgaagc taatgaagtc gcaattgatg aacttcgtca attaggttta
    23641 actattgact attccaatga tcatggtaaa catgaatggt attactggaa tcaacaatta
    23701 gaaaaagtct tagaatggct ccctgttgat tatgtcaaag aagagcgcct ttcttagtat
    23761 tagctaaatc gttgaaatcc aagtaaagtc ttgttatact aaatcattaa gttttgggaa
    23821 attagacaca gaaggtgaga taaaaaatga gaacactatt tagaatgata tttgctattc
    23881 caaagtttat ctttagattg atttggaata tcatttgggg aatattcaag acagttcttg
    23941 ttattgcgat tattttattt ggcttgtatt actatgcgaa tcacagtcaa tcagaatttg
    24001 ctaatcaact tagtgacatt attcagacag gaaaaacatt tttaaatttt gctgacacta
    24061 atcagctcaa aaattccttt gctaatctag ctacggataa tgttcatcat tcagaaaata
    24121 ctagatggga taaaaatcag gcaactatct atatagcaac tagagattct gagttggcct
    24181 ctgcttacaa gcaagcaatc tccaattgga atgcaacagg tgcttttgtg ttacaaacaa
    24241 cagataatcc taatgctgat attattgcta aggattactc agatgctaaa acacaagctg
    24301 caggtgtagc tgaaaccgag agaaatgctt taactaatcg tatttcaaag gtaacagtta
    24361 aattaaacac attttacctt aagaataaac agtttggtta tgaccatact aggattgtta
    24421 atacggccga acatgaatta ggacatgctt taggtctagg acataatgat caacaacatt
    24481 ccgttatgca gtcaaaaggt tcgcattatg gcattcaaga agttgatatt caaacgctaa
    24541 aaaacttata tgcataaaaa gatgagctaa taaactcatc ttttttgttt gaacacttat
    24601 tttaggtgat ataagtgcgt tagcaataga ttattaaaaa taaagaattt gactatcact
    24661 actaactaaa atgcataaag aacaaagaaa atgttttcaa aaatcactta aaaaatggta
    24721 taatgtgtct tgagatagtg ttttgaatag acttatcaaa aatattagat aacattaact
    24781 taaaagggga aaatatggac gaactaaaaa aacttgctgg agtgaccgct gcaaaatacg
    24841 ttaaaaacgg tatgattgtg ggtttaggga caggttctac agcatatttt tttgttgaag
    24901 aaattggacg ccgtgtcaaa gaagagggat tgcaagtcat aggcgttaca acgtcaaacc
    24961 gtacgactga gcaggcaaga ggattaggta ttccgttgaa atcagcagat gatattgatg
    25021 tcattgatgt cactgtggat ggagcagatg aagtagaccc tgattttaat ggtatcaagg
    25081 ggggcggtgg tgcgctgctc atggaaaaaa tagtagctac tccaactaaa gaatatatct
    25141 gggttgtcga tgagagcaaa cttgttgaaa cgttaggtgc atttaaattg cctgttgagg
    25201 ttgttagata tggttctgag cgtctttttc gggtttttaa atcaaaaggt tattgtcctt
    25261 ctttccgtga aacagaaggt gaccgcttta tcactgatat ggggaactac atcatcgacc
    25321 tagatttgaa aaaaatagag gatccaaagc agttagcgaa tgaacttgac catacagttg
    25381 gtgttgttga acatggttta tttaatggta tggttaataa agttatagta gcaagcaaaa
    25441 atggtcttga catacttgaa aaataattat ttaaataatc ggttttagga gctaaccgta
    25501 acctccttag aaagaagata taatgtcaca atttgatcgc attcacttag ttgtcttaga
    25561 ttcagtgggt attggagcgg caccagatgc caatgatttt gtcaatgcag gagttcctga
    25621 tggtgcttca gacacgcttg gccacatttc aaaaacagtt ggacttgcgg tgccaaacat
    25681 ggctaaaatc ggacttggta atattcctcg tccccaagcg cttaagaccg tccctgcaga
    25741 agaaaatcca agtggctacg cgacgaagtt acaagaagta tcacttggta aggatacaat
    25801 gacagggcat tgggagatca tgggcttaaa tattacagag ccatttgata ctttctggaa
    25861 tggttttcca gaagatatta tcacaaagat tgaggatttt tcaggacgta aagttattcg
    25921 tgaagcgaat aaaccttatt cagggacagc tgttattgat gacttcggac cacgccaaat
    25981 ggaaactggt gagctcatca tctatacctc agctgatcca gtcctacaaa ttgcagctca
    26041 tgaggatatc atccctctag aagaactcta ccgtatttgt gaatatgctc gta
//
LOCUS       DBJYSH010000010        25956 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.010, whole genome shotgun sequence.
ACCESSION   DBJYSH010000010 DBJYSH010000000
VERSION     DBJYSH010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 25956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25956
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.010"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            complement(<1..1011)
                     /locus_tag="ACUBV3_000357"
     CDS             complement(<1..1011)
                     /locus_tag="ACUBV3_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HHU3846893.1"
                     /translation="MYLIEPIRNGKRITDGAIALAMQVYILQNVFLDDDILFPYYCDP
                     KVEIGKFQNAVIETNQEYLKEHDIPVVRRDTGGGAVYVDSGAVNICYLMKDHGQFGDF
                     KRAYEPAIKALKTLGASSVEMRGRNDLVIDGKKVSGAAMTIVNGRIYGGYSLLLDVDF
                     DAMEKVLNPNRKKIESKGIKSVRSRVGDIRSHLSEDYRHITTDQFKDLMVCQLLHIDH
                     IDQAKRYHLTEKDWAAIDALADEKYKNWDWNYGNSPQYSYHRDARFPSGTYDFHLEIE
                     KGIITNCRIYGDFFSSKDISDIENLLIGCPMKEELVLEKLSTLSLEDYFGQTSPEEIK
                     AVL"
     gene            complement(1038..1916)
                     /locus_tag="ACUBV3_000358"
     CDS             complement(1038..1916)
                     /locus_tag="ACUBV3_000358"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - histone deacetylase activity,
                     NAD-dependent [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family NAD-dependent protein deacylase"
                     /protein_id="HHU3846894.1"
                     /translation="MTVWKTLEKTNHSQSEILSQLIEESDAIVVGIGAGMSAADGFTY
                     IGPRFEEAFPDFIAKYQLLDMLQASLYDFEDWEEYWAFQSRFVALNYLDQPVGQAYLD
                     LKDILAKKEYHIITTNADNAFAVADYNLEKVFHIQGEYGLWQCSQHCHQQTYRNDQAI
                     RQMIAQQKDMKIPSNLIPKCPKCDQPFEINKRNEEKGMVEDADFHAQRQRYENFLSQH
                     QNDKVLYLEIGVGHTTPQFIKHPFWRFVSLNENSLFVTLNHKHYRIPQKIRSRSVQLT
                     QHIAELIAEAKKEIIG"
     gene            complement(1885..2703)
                     /locus_tag="ACUBV3_000359"
     CDS             complement(1885..2703)
                     /locus_tag="ACUBV3_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-ADP-ribose hydrolase"
                     /protein_id="HHU3846895.1"
                     /translation="MPNQKQLLLAMIEYLQSEKLTDVDDLRTTDLQTVWRGLVNQQDP
                     QNISQEYLSLEDRYLSHWWNTQKVKTIDVCHQTVYSNVFTYHGDICLLQVDAIVNAAN
                     SKLLGCFIPNHHCIDNQIHTFAGSRLRLACHQLMTQQGRMEAVGQAKLTESYHLPCKY
                     VIHTVGPYVKVDQKPSRIREDLLKSSYKSCLQLAAQANLKTIVFPCISTGEFGFPNQR
                     AAELAVQEILEWQRENQHKLYIIFNTFTPKDQEIYQKLLLKEEIDDSVEDSRKN"
     gene            complement(2696..3028)
                     /locus_tag="ACUBV3_000360"
     CDS             complement(2696..3028)
                     /locus_tag="ACUBV3_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HHU3846896.1"
                     /translation="MKKIANYLLIEKNEELYTISLTPELQDDLGTVGYVEFTDDTNLE
                     VDDVILNIEASKTVMAILSPLTGKVVKVNTAASQEPTLLNSEKADENWLVVLTDVDYA
                     AFEALENA"
     gene            complement(3057..4043)
                     /locus_tag="ACUBV3_000361"
     CDS             complement(3057..4043)
                     /locus_tag="ACUBV3_000361"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MsnO8 family LLM class oxidoreductase"
                     /protein_id="HHU3846897.1"
                     /translation="MKLSVLDYGLIDYGKTASDAIQETILLSQEAEQLGYHQFWVAEH
                     HGVKAFSISNPELMIMHLANQTKSIKIGSGGIMPLHYSSFKLAETLKTLETCHPNRVS
                     IGLGNSLGTVKVSNALRSLHKAHDYEEVLEELKSWLIDESSSKEPLVQPTLSSFPDLY
                     VLGSGQKSAYLAAKLGLGFTFGVFPFMDKDPLTEAKKLSSLYYHQFEEYYPNKSPNLM
                     VAPFVVIADTSEEAENIAKTLDIWMLGNKDFNEFATFPTIEEANHYQLTPEQKAKIKS
                     NRHRMIVGDPKQVKESLDALVNASQAEELLLIPLVPGLDQRIKSLKLLSQLY"
     gene            complement(4040..5239)
                     /locus_tag="ACUBV3_000362"
     CDS             complement(4040..5239)
                     /locus_tag="ACUBV3_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HHU3846898.1"
                     /translation="MNNVQGNLFRPLTLPNGLSLENRFVLSPMVTNSSTSEGFVTDDD
                     IAYAVRRAKSAPLQITGAAYITEYGQLFEYGFSVSKDEDIPGLTKLAKAMKSKGAKAV
                     LQLTHAGRFSSHTLARHGYVYGPSPMQLQSPYPHQVKELTHKDILRIIDEYVQATRRA
                     IQAGFDGVEISSAQRLLIQTFFSTFSNQRKDEYGPQTLTNRCRLGLEVFKAVQKVIRE
                     EAESDFILGFRATPEETRGSQIGYSIEEFMEFLEKILAIAQVDYLAIASWGHDVFRNT
                     IRSEGVYKGQLVNQVIFEHFGDRVPIMATGGINSASKVFEALQHAHMIGASTPLVVDP
                     EFLQKIKAKRSDQINLRIKVNDLEGLAIPKASFKDIVPLMDYGESLPKEAREVFRELR
                     SNYREER"
     gene            complement(5232..6068)
                     /locus_tag="ACUBV3_000363"
     CDS             complement(5232..6068)
                     /locus_tag="ACUBV3_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HHU3846899.1"
                     /translation="MEWQDLAQLPVSIFKDYVTDAQDAEKPFIWTEVFLREINRSNQE
                     IILHIWPMTKTVILGMLDRELPHLELAKKEIISRGYEPVVRNFGGLAVVADEGILNFS
                     LVIPDVFERKLSISDGYLIMVDFIRSIFSDFYQPIEHFEVETSYCPGKFDLSINGKKF
                     AGLAQRRIKNGIAVSIYLSVCGDQKGRSQMISDFYKIGLGDTGSPIAYPNVDPEIMAN
                     LSDLLDCPMTVEDVIDRMLISLKQVGFNDRLLMIRPDLVAEFDRFQAKSMANKGMVSR
                     DE"
     gene            6222..6905
                     /gene="coaB"
                     /locus_tag="ACUBV3_000364"
     CDS             6222..6905
                     /gene="coaB"
                     /locus_tag="ACUBV3_000364"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001873872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HHU3846900.1"
                     /translation="MKILITSGGTTEKIDTVRSITNHATGALGKIIAEKYLREGHQVT
                     LVTTKNAVKPESATNLSIFEIEDVDSLIKTLKPLVKEHDILIHSMAVSDYTPVYMADF
                     EKVKSSDDLDTFLRKDNHEGKISSESEYQVLFLKKTPKVISLVKKWNPQITLVGFKLL
                     VNVTKENLFKVARHSLIKNKATFILANDLIDITSKHHIAYLLDHDNVYKATTKEDIAQ
                     LIYEKVKKI"
     gene            6902..7444
                     /gene="coaC"
                     /locus_tag="ACUBV3_000365"
     CDS             6902..7444
                     /gene="coaC"
                     /locus_tag="ACUBV3_000365"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HHU3846901.1"
                     /translation="MIKRITLAVTGSISAYKAADLTSQLTKIGYDVHIIMTQAATQFI
                     TPLTLQVLSKNPIHLDVMDEHNPKIINHIELAKRTDLFIVAPASANTIAHLAYGFADN
                     IVTSVALAMPDETPKLIAPAMNTKMYHNTITQRNIDILKKIGYQEIEPRISLLACGDT
                     GQGALADISTILKCIQEVAN"
     gene            7490..8062
                     /locus_tag="ACUBV3_000366"
     CDS             7490..8062
                     /locus_tag="ACUBV3_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HHU3846902.1"
                     /translation="MKKKNKSSNIAIIAIFFAIMLVIHFLSSFIFSFWLVPIKPTLMH
                     IPVIIASIAYGPRIGATLGALMGGISVANSSIVLLPTSYLFSPFVENGNFYSLIIALV
                     PRILIGIIPYFVYKLLHNRFGLAISGAIGSLTNTVFVLSGIFIFFSSTYNGNIKLMLA
                     GIISSNSLAEMVIAAIIVYLTVPRILNIKH"
     gene            8172..9890
                     /locus_tag="ACUBV3_000367"
     CDS             8172..9890
                     /locus_tag="ACUBV3_000367"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HHU3846903.1"
                     /translation="MTYTENFQKWLDFEQLPDYLRQELLSMDEKTKEDAFYTNLEFGT
                     AGMRGYIGAGTNRINIYVVRQATEGLAKLIETKGEEAKKRGVAIAYDSRHFSPEFAFE
                     SAQVLAQHGIKSYVFEALRPTPELSFAVRHLNAYAGIMVTASHNPAPFNGYKVYGQDG
                     GQLPPADADALTDFIRAIENPFAVELADLDESKSSGLIQVIGEDVDIEYLREVKDVNI
                     NQDLINNFGKDMKIVYTPLHGTGEMLTRRALAQAGFESVVVVESQAKADPDFSTVKSP
                     NPESQAAFALAEELGREVNADVLVATDPDADRLGVEIRQPDGSYKNLSGNQIGAIIAK
                     YILEAHKTAGTLPENAALAKSIVSTELVTKIAESYGATMFNVLTGFKFIAEKIQEFEE
                     KHNHTYMFGFEESFGYLIKPFVRDKDAIQAVLLVAEIAAYYRSRGLTLADGIDEIYKE
                     YGYFAEKTISVTLSGVDGAAEIKKIMDKFRENGPKQFNNTDIVLLEDFQKQIATKNDG
                     TISNLTTPPSNVLKYTLADDSWIAVRPSGTEPKIKFYIATVGNDLADAETKIANIEKE
                     ITTFVN"
     gene            complement(9954..10151)
                     /locus_tag="ACUBV3_000368"
     CDS             complement(9954..10151)
                     /locus_tag="ACUBV3_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3846904.1"
                     /translation="MKKRLQLLLLIFISAVSWFISNQLATKNTVLSVLIGLVAVIGIT
                     WLIVSLLYYLIVKLIAVIFHD"
     gene            complement(10165..11898)
                     /locus_tag="ACUBV3_000369"
     CDS             complement(10165..11898)
                     /locus_tag="ACUBV3_000369"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HHU3846905.1"
                     /translation="MKASHPKKIQKKLVQDLLSKKSLVGMALLGTVVQVCLTVYLPVL
                     IGQAVDVVLSPHSMILLLPIMWKMVAVILANTIIQWINPLLYNRLIFHYVASLRKAVM
                     EKLNLLPIAYLDKRGIGDLISRVTTDTEQLSNGLLMVFNQFFVGLLTILVTIFSMAKI
                     DLLMLFLVLFLTPLSLFLARFIAKKSYHLYQNQTASRGRQTQFIEEMVSQESLIQAFS
                     AQEESSDHFRTINQEYANFSQSAIFYSSTVNPSTRFINSLIYGFLAGIGALRIMSGAF
                     SVGQLITFLNYVNQYTKPFNDISSVLSEMQSALACAERLYSILEESSPNITGTEKLDS
                     STVKGQIDFKNVIFGYNKSKPLLNGINLHIPAGAKVAIVGPTGAGKSTLINLLMRFYE
                     VDGGNILLDGKPITDYEPSQLRQEIGMVLQETWLKSATIHDNIAYANPKASREEVIEA
                     AKAANADFFIKQLPNGYDTYLEDAGDSLSQGQCQLLTIARIFLKLPRILILDEATSSI
                     DTRTEVLVQEAFQMLMKGRTSFIIAHRLSTIQTADIILVMVSGEIVEVGNHSELIAQK
                     GIYYQMQNAQK"
     gene            complement(11899..13620)
                     /locus_tag="ACUBV3_000370"
     CDS             complement(11899..13620)
                     /locus_tag="ACUBV3_000370"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000826931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HHU3846906.1"
                     /translation="MKRLTYYFKGYIKETIFGPLFKLLEASFELLVPIVIAKMIDETI
                     PRGDRSSLLLQIGLIFFLAAVGVVVAITAQYYSSKAAVGYTRQLTEDLYQKVMSLGKK
                     DRDELGTASLITRLTADTFHIQTGLNQFLRLFLRAPIIVFGAIIMAFSISPSLTIWFL
                     IMVVTLFIIVFVMSRLLNPIYLKIRTSTDYLVKLTRQQLQGVRVIRAFNQVDRESEAF
                     NDINYHYTNLQLKAGRLSSLVTPLTFLVVNITLVVIIWRGNLNIANHLLSQGMLVALI
                     NYLLQILVELLKMTMLVTSLNQSYISAKRIIAVFERPSEIIDDKLEPKYSNKALEVQE
                     MAFSYPNSSEKALSDITFSMNVGETLGIIGGTGSGKSTLVNLLLHIYKVQEGDIDIYH
                     QGKSPDTISNWRSLVRVVPQNAQLFKGTIRSNLSLGLGKVSEEKLWTALEIAQASDFV
                     KEKDGQLDAPVESFGRNFSGGQRQRLTIARALVQDKIPFLILDDATSALDYLTEARLF
                     KAIIKHFNQTNLIIVSQRINSIQNADRILLLDKGKQVGFDNHQSLLAHNKVYKSIYHS
                     QNFKEEE"
     gene            complement(13632..14234)
                     /locus_tag="ACUBV3_000371"
     CDS             complement(13632..14234)
                     /locus_tag="ACUBV3_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HHU3846907.1"
                     /translation="MFKFLKRLIALIIIIFIGYRLVIIHENVKKVLQYHDLVQNTLAE
                     NGSEANVHLVLSMIYTETKGDAIDVMQSSESISGTTNSITDSHTSIKHGVTLLSQNIS
                     QAKKAKVDVWTAVQAYNFGSSYIDYVADHGGENSIELAKNYSKNVVAPSLGNYNGDTY
                     FYYHPLALISGGKLYKNGGNIYYSREVQFNLYLIKIMELF"
     gene            complement(14236..15213)
                     /locus_tag="ACUBV3_000372"
     CDS             complement(14236..15213)
                     /locus_tag="ACUBV3_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014677993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HHU3846908.1"
                     /translation="MMDFYIKQIIIHQFSPNDTELVLSDTPLTLTPRIDDYFRKKLSK
                     VFSDEAKRGYFGEDNVFMSHLQDDLYVSSCQIAQLWKEEFVISEDQKTNDLVFIQFDK
                     DGMEHFAFLRISLKEQFAHVSENQEQPITITQNNLPSAAQTPDEALVVNKSSKQYYLI
                     EKRIKHNGSFANYFSENLLQVQPEQSVKKSIKMVEQTAQKIAENFNKDDFSFQSKMKS
                     AIYKNLEEEQELSPEKLADQLFDDNLTARLTFVDELKDAIPGSVQVSDIDHSRQIKKL
                     ENQKLSLSNGIELIVPNNVYQDAESVEFIQNPDGTYSILIKNIQDIQNK"
     gene            complement(15218..16474)
                     /gene="glyA"
                     /locus_tag="ACUBV3_000373"
     CDS             complement(15218..16474)
                     /gene="glyA"
                     /locus_tag="ACUBV3_000373"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HHU3846909.1"
                     /translation="MIFDKDNFKEFDQELWQAIHDEEIRQQNNIELIASENVVSKAVM
                     AAQGSVLTNKYAEGYPSHRYYGGTDCVDVVESLAIERAKMLFNAEFANVQPHSGSQAN
                     AAAYMALIEPGDTVLGMDLAAGGHLTHGASVSFSGKTYHFVSYSVDPKTEMLDYDNIL
                     KIAQETQPKLIVAGASAYSRIIDFEKFRQIADAVDAYLMVDMAHIAGLVASGHHPSPI
                     PYAHVTTTTTHKTLRGPRGGLILTNDEAIAKKINSAVFPGLQGGPLEHVIAAKAVALK
                     EALDPSFKIYGEDIIKNAQAMAKVFKEDDDFHLISDGTDNHLFLVDVTKVIENGKKAQ
                     NVLEEVNITLNKNSIPFERLSPFKTSGIRIGTPAITSRGMGVEESRRIAELMIKALKN
                     HENQDVLTEVRQEIKSLTDAFPLYEN"
     gene            complement(16566..17162)
                     /locus_tag="ACUBV3_000374"
     CDS             complement(16566..17162)
                     /locus_tag="ACUBV3_000374"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HHU3846910.1"
                     /translation="MNDLGQILEDHGAVIMPTETVYGIFAKALSEEAVNHVYELKKRP
                     RDKAMNLNICDFETILKYSKNQPTYLKQLYDAFLPGPLTIILEASQEVPDWINSGLSS
                     VGFRMPKHPVTLDMIANHGPLIGPSANISGCDSGRVFSEIQKQFNHQVLGIEDDKALT
                     GVDSTIIDLSGDRVKILRQGAITQEVLTATIPELIFEN"
     gene            complement(17155..17985)
                     /gene="prmC"
                     /locus_tag="ACUBV3_000375"
     CDS             complement(17155..17985)
                     /gene="prmC"
                     /locus_tag="ACUBV3_000375"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001104950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HHU3846911.1"
                     /translation="MNYAQLIKHYEQLLEACGEEVENFIYVLKDLKQWSTTDYLLNQN
                     SSVSDTDQQLMENIFQQLKKHRSPQYITGKAYFRDLIFFVDERVLIPRPETEELVDLI
                     LSENKVEDCSVLDIGTGSGAIAISLKKERPSWDVLASDISVSALDLAKENANNCDAEV
                     TFIESDVFSNISDKFDIIVSNPPYISYNDKDEVGKNVLASEPHSALFADEEGLAIYRK
                     IIENSREYLQPRGKLYFEIGYKQGDDLRSLLKRYFPNNRCRVLKDIFGKDRMVVLDNE
                     "
     gene            complement(17985..19064)
                     /gene="prfA"
                     /locus_tag="ACUBV3_000376"
     CDS             complement(17985..19064)
                     /gene="prfA"
                     /locus_tag="ACUBV3_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HHU3846912.1"
                     /translation="MNIYDQLQAVEDRYEELGELLSDPDVVSDTKRFMELSREEANTR
                     ETVTAYREYKQVIQNISDAEEMIKDASGDAELEEMAKEELKESKAAKEEYEERLKILL
                     LPKDPNDDKNIILEIRGAAGGDEAALFAGDLLTMYQKYAETQGWRFEVMESSVNGVGG
                     IKEVVAMVSGQSVYSKLKYESGAHRVQRVPVTESQGRVHTSTATVLVMPEVEEVEYEI
                     DQKDLRVDIYHASGAGGQNVNKVATAVRMVHIPTGIKVEMQEERTQQKNRDKAMKIIR
                     ARVADHFAQIAQDEQDAERKSTVGTGDRSERIRTYNFPQNRVTDHRIGLTLQKLDTIL
                     SGKMDEVIDALVMYDQTQKLEALNK"
     gene            complement(19099..19668)
                     /locus_tag="ACUBV3_000377"
     CDS             complement(19099..19668)
                     /locus_tag="ACUBV3_000377"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000068112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HHU3846913.1"
                     /translation="MAQLYYKYGTMNSGKTIEILKVAHNYEEQGKPVVIMTSALDTRD
                     EFGVVSSRIGMRREAVPISDDMDIFSYIQNLPQKPYCVLIDECQFLSKKNVYDLARVV
                     DDLDVPVMAFGLKNDFQNNLFEGSKHLLLLADKIDEIKTICQYCSKKATMVLRTENGK
                     PVYEGDQIQIGGNETYIPVCRKHYFNPDI"
     gene            19806..19988
                     /locus_tag="ACUBV3_000378"
     CDS             19806..19988
                     /locus_tag="ACUBV3_000378"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HHU3846914.1"
                     /translation="MPFVKIDLFEGRSQEQKNELAREVTEVVSRIAKAPKENIHVFIN
                     DMPEGTYYPQGELKKK"
     gene            20137..21075
                     /locus_tag="ACUBV3_000379"
     CDS             20137..21075
                     /locus_tag="ACUBV3_000379"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001177180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HHU3846915.1"
                     /translation="MQLSHSLRLMGTTIDIQINSKNAQKQIQEVIELLELYKNRFSAN
                     DFNSELMAINNNAGIKPIQVHPDLFELITIGKEHSLARPSNLNIAIGPLVQTWRIGFS
                     DAKLPSPSEVSEAMILSDPTHILLDSKNQSVFLNQIGMKIDLGALAKGYIADKIMTYL
                     KNEMIDSAIINLGGNVLVHGDNPNRSEGYWVIGIQHPKKKRGQNIGAVKIKNQSVVTS
                     GTYERRLIIDDKEYHHIFDRQTGYPIQTEMASISIVSKQSVDCEIWTTRLFGLSIKEA
                     LDILNAVSYIEGIIITKDDRIYLSDGLKHHFQLFYH"
     gene            21093..21695
                     /locus_tag="ACUBV3_000380"
     CDS             21093..21695
                     /locus_tag="ACUBV3_000380"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase [NAD(P)H] activity
                     [Evidence IEA];
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HHU3846916.1"
                     /translation="MKLIGIVGTNSNKSTNRQLLQYMQQHFADKAEIELIEVKDLPLF
                     NKPADKNVPQVILDIAAKIEEADGVIIGTPEYDHSIPSALMSVLAWLSYGIYPLLNKP
                     VMITGASYGTLGSSRAQLQLRQILNAPELKASVLPDEFLLSHSLQAFDKDGNLHDIET
                     SQKLDAIFDDFRLFVKITGKLSNARDLLQKEAENFDWESL"
     gene            21719..22954
                     /locus_tag="ACUBV3_000381"
     CDS             21719..22954
                     /locus_tag="ACUBV3_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HHU3846917.1"
                     /translation="MKFVGIVGSNAEQSYNRMLLEFIRKNFKTKFELEVLEIDDIPMF
                     NQVQNWEESFQLRLLNNKITRADGVIIATPEHNHTITAALKSVLEWLSFAVHPLENKP
                     VMIVGASYYDQGTSRAQIHLRKILDAPGVNAYTLPGNEFLLGKAKEAFDDNGNIINPG
                     TVQFLETCLDNFIKYVGVVSSLRKPKAIESEDLYCQGQIDTTITSIDPDDPDWLDKAA
                     EKVNAVEGDAYVKLDNGLLTVNQLNMFLNAMPFELTYADDNNQFLYYNNNHPDPDTML
                     GKRVREQVGNRMATVHGSLPPARMKNVEWVIGTLRNGNQEYVRTVVPGTPPEVINTHN
                     YQAMYYPDGSYAGINEIIFNFKPWLDWYLQTTGQRLVGGTTAAPGASAVDATSSASTS
                     TEDAPVDQATDATSGASEH"
     gene            23053..23841
                     /locus_tag="ACUBV3_000382"
     CDS             23053..23841
                     /locus_tag="ACUBV3_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HHU3846918.1"
                     /translation="MSLFQEKIAYNCAKKEALYKESLGRYALRSMLAGAYLTMSTAAG
                     IVAADTIGKISPALSGFVFAFIFSFGLIYVLIFNGELATSNMLYLTAGAYNKNISWKK
                     AITILIYCTFFNLVGACILAWLFNQSYSFQHLTNDSFLGHVVAKKLGKPSSGAFLEGI
                     IANMFVNLAILAYMLLKEESAKMTVILSAIFMFVFLSNEHLIANFASFMLAAFSHIEH
                     IKGFTLLNVIRQWTLVFFGNWIGGGVFIGLAYAWLNKTKTSHHE"
     gene            complement(23940..25214)
                     /locus_tag="ACUBV3_000383"
     CDS             complement(23940..25214)
                     /locus_tag="ACUBV3_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015851 - nucleobase transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HHU3846919.1"
                     /translation="MKFNEQSNSQAALLGLQHLLAMYAGSILVPIMIASALGYNAKQL
                     TYLIATDIFMCGIATLLQLRLSKHFGVGLPVVLGCAFQSVAPLSIIGAQQGSGYMFGA
                     LIASGIYVVLVAGIFSKVANFFPPIVTGSVITTIGLTLIPVAMGNMGDNAKEPSLQSL
                     TLSLVTIGVVLLINIFAKGFLKSISILIGLISGTILAAFMGLVDASVVAEAPLVHIPK
                     PFYFGAPRFEFTSILMMCIIATVSMVESTGVYLALSDITNDKLDSKRLRNGYRSEGLA
                     VLLSGLFNTFPYTGFSQNVGLVQISGIRTRKPIYFTALFLVILGLLPKFGAMAQMIPS
                     PVLGGAMLVLFGMVALQGMKMLNQVDFEHNEHNFIIAAVSIAAGVGFNETNLFISLPN
                     TLQMFLTNGIVISTLTAVVLNIILNGLPKKLI"
     gene            complement(25214..25795)
                     /locus_tag="ACUBV3_000384"
     CDS             complement(25214..25795)
                     /locus_tag="ACUBV3_000384"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HHU3846920.1"
                     /translation="MKLLEERILKDGDVLGENILKVDSFLTHQVNFELMQEIGKVFAD
                     KYKEAGITKVVTIEASGIAPAVYAAQALGVPMIFAKKAKNITMTEGILTAEVYSFTKQ
                     VTSQVSIVSRFLSNDDTVLIIDDFLANGQAAKGLLEIIGQAGAKVAGIGIVIEKSFQD
                     GRDLLEKTGVPVTSLARIKAFENGRVVFAEADA"
BASE COUNT         8463 a         4954 c         4134 g         8405 t
ORIGIN      
        1 taaaactgct tttatttctt ctggactagt ctgaccaaaa taatcttcta atgataaagt
       61 acttaatttt tccaagacta attcttcttt catagggcaa ccaatgagaa ggttttcaat
      121 atctgaaata tctttagaac tgaaaaagtc accataaata cggcaattcg taataatacc
      181 tttctcaatt tctaagtgaa aatcgtaagt tcctgacgga aaacgagcat ctctgtgata
      241 cgaatactgt ggtgagttac cataattcca atcccaattc ttatattttt catcagctag
      301 agcgtcaatc gcagcccaat ctttttcggt tagatgatag cgttttgcct gatcaatatg
      361 atcaatatgt aataattgac aaaccattaa atctttaaat tgatcggttg tgatatgacg
      421 ataatcttct gacaagtggc tacgaatatc tcctactcga cttcggacag attttatccc
      481 tttagattca atttttttcc gattaggatt taacactttt tccatagcat caaaatcaac
      541 atctaataaa agagagtaac caccgtaaat acgaccgttt actatcgtca ttgctgcacc
      601 tgatactttt ttaccatcaa taactaagtc atttcgtccc ctcatttcaa cactagaagc
      661 acctaaagtt tttagggcct taatagctgg ctcataagct cgtttgaaat caccaaattg
      721 tccatgatct ttcattagat agcaaatatt tacagcacct gagtcaacgt aaacagctcc
      781 tccaccagta tctcgacgaa caacaggaat atcatgttct ttcaaatatt cttgatttgt
      841 ctctataact gcattctgaa attttccgat ttcaacttta ggatcacaat agtatggaaa
      901 taaaatatca tcatctaaaa aaacattttg taaaatataa acttgcatgg caagtgcaat
      961 cgcaccgtct gtgatacgct taccatttct aattggttca ataagataca tttaggtctc
     1021 cttttatagc aatttagtta tccaataatt tcttttttcg cttcagcaat taattcagcg
     1081 atatgctgtg tcagttgcac actccttgag cgaatctttt gaggaatgcg ataatgcttg
     1141 tgattaagag taacaaataa agagttttca tttaaagaga cgaatcgcca aaacggatgc
     1201 ttaataaatt gtggcgtggt atgtccaact ccaatctcta gataaagaac tttgtcattt
     1261 tgatgttgtg ataagaaatt ttcataacgt tgtcgttgag catgaaagtc agcatcttcc
     1321 accatacctt tttcttcgtt acgtttatta atttcaaacg gttgatcaca ctttgggcat
     1381 ttggggatta agttactagg aatcttcata tccttttgtt gtgcaatcat ttgacgaatt
     1441 gcctgatcat ttcgataagt ttgttgatga caatgttgac tacactgcca taagccatat
     1501 tcaccttgaa tatgaaaaac tttttctaaa ttataatcag ccacagcgaa ggcattatca
     1561 gcattagttg taataatatg atattctttt ttcgctaaaa tatcctttaa atcaagataa
     1621 gcttgtccta ccggttgatc taaataatta agtgccacaa aacgactttg gaaggcccaa
     1681 tattcttccc aatcttcaaa gtcataaaga cttgcttgca acatatctaa tagttgatat
     1741 ttagcaataa aatccggaaa agcttcttca aatctggggc caatataggt aaatccatcc
     1801 gctgcagaca tgcctgcacc aataccaact acaatagcat ctgattcttc tattaactga
     1861 cttagtattt ctgattgaga atgattagtt ttttctagag tcttccacac tgtcatctat
     1921 ttcctctttt aataataatt tttgataaat ctcttggtct ttaggtgtga atgtattgaa
     1981 aataatatag agtttgtgct gattttcgcg ttgccactct aatatctctt gaacagccaa
     2041 ttcagcggct ctttgattag gaaaaccaaa ttctcccgtt gatatacaag ggaagacaat
     2101 tgttttaaga ttagcttgag ctgccaattg taagcaggat ttataagaag actttaataa
     2161 atcctctctt atacgagatg gcttttgatc aacttttaca taaggtccga ctgtatgaat
     2221 aacatactta caaggaagat gataggattc ggtaagttta gcttgtccta cggcttccat
     2281 tcgtccttgt tgtgtcatca attgatgaca tgctaaacgt aatcgtgaac ctgcaaacgt
     2341 atgaatctga ttgtcaatac aatggtgatt aggaataaaa caacctaaca acttactatt
     2401 tgctgcatta acaatagcat ccacttgtaa taagcaaata tccccatggt aggtaaatac
     2461 atttgaatat accgtttgat gacagacgtc tatcgttttt actttttgag tattccacca
     2521 atgtgacaaa tacctatctt ctaaagacag gtactcttga ctaatgtttt gaggatcttg
     2581 ttgattaact agaccacgcc aaaccgtttg caaatcggtg gtcctcaagt catcaacgtc
     2641 tgttaatttt tctgactgaa gatactcaat catggctagc aatagttgtt tttgattagg
     2701 cattttctaa agcttcaaaa gcagcataat caacatctgt taacacaacc aaccaatttt
     2761 cgtcagcttt ttcagaattt aacaaagttg gttcttgact tgcagctgta ttaactttaa
     2821 caactttacc tgtaagagga gataaaattg ccataaccgt ctttgaagct tcaatattta
     2881 aaataacatc atcaacttct aaatttgtat catctgtgaa ttccacatat ccaacagttc
     2941 ccaagtcatc ttgaagttcg ggagttaagc tgatagtata aagttcttca tttttttcaa
     3001 ttaataagta gttcgcaatt tttttcatga gtaatttcct ttctgagaaa acgtgattaa
     3061 tataactgtg ataataattt taaggatttg attcgttgat caagtcctgg aacaagagga
     3121 atcaaaagta gttcttctgc ttgactagca ttaactaaag cgtcaaggct ttctttaact
     3181 tgttttggat cacctacaat catacggtgg cgatttgatt taatttttgc tttttgttca
     3241 ggagtcaatt ggtaatgatt agcttcttca atagtaggaa atgttgcaaa ttcattgaag
     3301 tctttatttc ctagcatcca gatatctaaa gtctttgcta tgttttcagc ttcttctgaa
     3361 gtatcagcta taactacaaa tggagctacc atcaaattag gagacttatt aggataatat
     3421 tcttcaaact gatgataata aagagatgaa agctttttag cttctgtcaa tgggtctttg
     3481 tccataaaag gaaaaacacc gaaggtaaag cctaagccaa gtttagccgc taaataagct
     3541 gatttttgac cactccccaa cacatataag tctgggaagc tagaaagagt cggttgaact
     3601 aatggttcct tactggatga ttcatcaata agccatgact tcaattcctc cagtacctct
     3661 tcgtaatcat gtgctttatg taagctacga agtgcatttg aaactttaac tgtccctagt
     3721 gaatttccta aaccaatact tactcgattc ggatgacatg tctctaatgt cttgagagtc
     3781 tccgcgagtt taaaactact atagtgcaga ggcattatac ctccagagcc aattttgata
     3841 gatttagtct ggttagccaa atgcattatc attaattctg gattgctaat actgaatgcc
     3901 ttaacaccgt gatgttcagc cacccaaaat tgatgatagc ctagttgctc cgcctcttgt
     3961 gataaaagaa tcgtttcttg tattgcatca cttgcagttt ttccataatc aataagccca
     4021 taatcaagga cacttaattt catctttcct ccctataatt gcttcgaagt tctctaaaaa
     4081 cttctcgagc ttcctttggt aaagattctc cataatccat caaaggaaca atatctttaa
     4141 aggaggcttt tggaatcgcc aaaccttcta aatcattcac tttaatcctt aaattaatct
     4201 gatcagaacg cttagctttt attttttgta aaaattccgg atcaactact aagggcgtgg
     4261 aagctcctat catatgagca tgttgtaagg cttcaaacac ctttgaagca ctattaatac
     4321 ctccagttgc cataattgga actctatcac caaaatgttc aaatataact tgattaacca
     4381 gctgcccttt gtaaactccc tcagaacgta tagtatttct aaagacatca tgcccccagc
     4441 ttgcaatggc aagataatct acctgagcaa tcgctaaaat tttttcaaga aattccataa
     4501 actcttcaat gctatatcca atttgtgaac cgcgagtttc ttcaggcgtc gctctaaaac
     4561 caaggataaa atctgactcc gcttcttctc tgataacttt ttgtaccgct ttaaaaactt
     4621 ctaagcctaa acgacagcga ttagtcaaag tttgaggacc atactcatct tttctttgat
     4681 ttgagaaggt tgagaaaaaa gtttgaatta gtaatcgttg tgcagaagat atttcaacac
     4741 catcaaatcc agcttgtata gctcgtcttg tagcctggac atattcatcg ataatcctta
     4801 gtatatcttt gtgtgttagt tctttaacct gatgtgggta tggcgattgc aattgcatag
     4861 ggctgggacc atatacatat ccgtgtcttg ccaaagtgtg acttgagaaa cgtccagcat
     4921 gcgtcagctg caaaacagct ttcgctccct tagacttcat tgctttagcc aatttagtca
     4981 aaccaggaat gtcctcatcc tttgaaacgc taaaaccata ttcaaataac tgaccatatt
     5041 ctgtgatata tgctgctcca gtaatttgta atggagcgga tttagcacga cgaacagcat
     5101 atgctatgtc gtcatcagtt acaaaacctt ctgatgtaga agaattagtt accataggtg
     5161 atagaacaaa acgattttct aagctcaatc cattgggaag tgttaatgga cgaaataaat
     5221 taccttggac attattcatc tctgctcacc atccccttat tagccataga cttagcctga
     5281 aatctatcaa actctgcaac taaatcgggt ctaatcatca gtaaacgatc attaaaacct
     5341 acttgtttaa ggctaatcaa catacgatca ataacatctt ctactgtcat aggacaatct
     5401 aatagatcag atagattagc cataatttca ggatctacat ttggataagc aattggacta
     5461 cccgtatcac ctagaccaat cttataaaaa tctgaaatca tttgactccg ccctttttga
     5521 tcgccacaaa cgctaaggta aattgatacc gcaataccat tctttatacg gcgctgagcc
     5581 aagccagcaa attttttgcc atttatacta agatcaaact taccaggaca ataggaggtc
     5641 tctacttcaa agtgctcaat aggttgataa aaatccgaaa atatacttct aataaaatcg
     5701 accattataa gatacccatc tgagatagac aattttctct caaaaacatc tggaataacc
     5761 aatgaaaaat ttaaaattcc ttcatcagct acaactgcga gacctccaaa attccgaaca
     5821 actggttcat aaccacgact gatgatttct tttttagcta attctaaatg tggtaattct
     5881 cgatctaaca tccccagaat gactgtctta gtcatcggcc aaatatgcaa aataatttct
     5941 tgatttgagc gattaatctc ccttaaaaat acttctgtcc atataaaagg tttttccgcg
     6001 tcttgagcat ctgtaacata gtctttaaaa atagatacag gtaactgcgc tagatcttgc
     6061 cactccaata aatctgacct cctttctcta gaaaactctg caagatttat tttatcgcaa
     6121 tcgcttgaaa ttttccagta agtttccctc aaattccatt actatcaaga agaaatatat
     6181 cttttattta cgaaaagcat ttttttatgt acaatatggc tatgaaaatt ttgattacat
     6241 caggtggaac taccgagaaa attgataccg ttaggagtat cacgaatcac gcaacaggag
     6301 ctttaggaaa aataattgca gaaaaatatt tacgagaagg acatcaggtg acactcgtga
     6361 ctactaaaaa tgcagtcaaa ccagaatctg ctaccaactt atccatattt gagattgagg
     6421 acgttgactc attaataaaa accttgaagc cattagtgaa agagcatgat attttaattc
     6481 atagcatggc agtgtctgac tatacccccg tctacatggc tgatttcgaa aaggttaaat
     6541 caagcgatga tttagatact tttttgcgca aagataacca cgaagggaaa atatcatctg
     6601 aatcagaata tcaagtcctt tttctcaaaa aaacaccaaa agttatcagc cttgtgaaaa
     6661 aatggaatcc tcaaattact ctagtaggtt ttaaactact tgtgaacgtt actaaagaaa
     6721 acctttttaa agttgctcgt catagcttaa tcaaaaataa agctacgttt attttagcta
     6781 atgacctcat tgatattacc tcaaaacatc acatagccta cctacttgat catgataatg
     6841 tgtataaagc taccaccaaa gaagatattg ctcaattaat ttacgagaag gtaaaaaaaa
     6901 tatgattaaa cgtattacac tagccgtcac aggttcaatt tcggcctata aagcagcaga
     6961 ccttacaagt caattaacta aaattggcta tgatgttcat atcataatga cacaagctgc
     7021 cacacaattt attacaccgt taactctgca agtattatca aaaaatccta ttcatttaga
     7081 tgttatggat gagcataacc caaaaatcat caaccatatc gaattagcaa aacgcacaga
     7141 tttatttatc gttgcacctg cctctgctaa tactattgct catctagcat atggcttcgc
     7201 tgataacatc gtaacaagcg ttgctcttgc tatgcccgat gaaactccaa aacttatcgc
     7261 accagcaatg aatacaaaga tgtatcataa tacaatcacc caacgtaaca ttgatatttt
     7321 gaaaaaaata ggttatcagg aaattgaacc tcgtattagt ttgcttgcct gtggtgatac
     7381 cggtcaagga gccctagctg atatctctac tatacttaaa tgtatacaag aagttgcaaa
     7441 ttaggctgta aatttgtata ctatttctag tttaatttaa ggagttataa tgaaaaagaa
     7501 aaacaaatcc tctaacattg ctataattgc aatctttttt gctattatgc ttgtcattca
     7561 ttttttgtca tcatttattt ttagtttttg gttagtccct attaaaccta ctttgatgca
     7621 tatcccagtt attattgcat ctatagccta tggacctcgt attggtgcaa ctctaggcgc
     7681 cttaatgggg gggatcagcg tagctaacag cagcattgtt ctattaccaa cgagttacct
     7741 cttctcacct tttgttgaaa atggtaattt ttattcgcta attattgcac ttgtaccacg
     7801 tattctaatc gggattattc cttatttcgt ttacaaatta ctacacaacc gctttggttt
     7861 ggctatctca ggtgctatag gctctctaac aaacacagta tttgttttat ctggaatttt
     7921 tatctttttt tcaagtactt ataatgggaa tatcaagcta atgctcgctg ggattatttc
     7981 atctaattca ttagctgaga tggtcattgc agctatcatt gtatatctaa ctgttcctcg
     8041 tattctcaat attaaacatt aaaaactggc caggaagaaa tcttcctggc ttttcttact
     8101 tatattttat agtcatattg aaaaaaacat gttataatga aagcgtttat aaatattgaa
     8161 ggagtatttc aatgacttac acagaaaatt ttcaaaaatg gttagatttt gagcagcttc
     8221 ctgactatct tcgtcaagaa ttactatcaa tggatgaaaa aacaaaagaa gatgcttttt
     8281 acacaaactt agaatttggt actgctggta tgcgtggtta tattggtgct ggtacaaatc
     8341 gtattaatat ttatgttgtc cgtcaagcaa ccgaaggatt ggcaaaatta atcgaaacta
     8401 agggagagga agctaaaaaa cgtggtgtag ccattgctta tgattcacgc cacttctctc
     8461 cagaatttgc ttttgagtct gcacaggtct tggcacagca tggaattaaa tcttacgtat
     8521 ttgaagctct tcgtccaaca ccagaactct cttttgctgt tcgccattta aatgcctatg
     8581 ctggtattat ggttactgca agccacaatc cagcaccttt caatggttat aaagtctatg
     8641 gacaagacgg aggtcaacta ccaccagctg atgctgatgc actaacagac tttatccgtg
     8701 ctattgaaaa tccatttgca gttgaacttg ctgatttaga tgaaagcaaa tcatcgggcc
     8761 ttattcaggt tatcggtgaa gatgttgata ttgaatatct tagagaggtt aaagatgtta
     8821 atatcaatca agatttaatc aataactttg gaaaagacat gaaaattgtc tatactcctc
     8881 tccacggaac tggtgagatg ttgacacgcc gtgcccttgc tcaagccggt tttgaatctg
     8941 ttgttgtagt agaatcacaa gctaaggcag atccagactt ttctactgtt aaatcgccta
     9001 accctgaaag ccaagctgcc tttgctcttg ctgaagaact cggacgagaa gttaatgcag
     9061 atgtcttagt agctactgat cctgatgcag accgcctagg cgttgaaatt cgccaaccag
     9121 atggttctta taaaaacctt tctggaaatc aaattggagc gattattgcg aaatacatcc
     9181 tggaagctca taaaacagca ggaacacttc cagaaaatgc tgctctcgct aaatcaatcg
     9241 tatcaacaga attagtaaca aaaattgccg aaagttatgg cgctacaatg tttaacgttt
     9301 taactggatt caaattcatc gctgagaaaa ttcaagagtt tgaagaaaaa cataaccaca
     9361 cctatatgtt tggttttgaa gagagcttcg gatatcttat taaaccattt gtccgtgata
     9421 aagacgctat tcaagcagtt cttttggttg ctgagattgc agcttattac cgttcgcgtg
     9481 gtttaacact tgcagatggt attgatgaaa tttacaaaga atatggttat tttgctgaaa
     9541 aaacgatttc agtaacactt tcaggagtgg atggagctgc tgaaattaag aagattatgg
     9601 acaaattccg tgaaaatggt ccaaaacaat ttaacaatac tgatattgta ttattagaag
     9661 atttccaaaa acaaatcgct actaaaaatg atggaacaat atctaactta acaactcctc
     9721 caagtaatgt tttgaagtac acgttagctg atgattcatg gattgcagtt cgcccttcag
     9781 gaactgaacc aaaaatcaag ttctatattg ctacggtcgg taatgattta gcagatgcag
     9841 aaacaaaaat cgccaatatt gaaaaagaga ttacaacatt tgtaaattaa taaaaaaagc
     9901 taggaactta ctcctagctt tttttatatc tatctaacga tgttctatta ttttcaatca
     9961 tgaaatataa ctgcaattaa cttcacgatc aaataataga gcagacttac aattaaccac
    10021 gttatcccaa taactgcaac taaacctatt aaaacagata ataccgtatt cttagttgct
    10081 agttggttac tgataaacca tgaaactgca gaaataaata tcagtaataa tagttgaagc
    10141 ctttttttca tataattcct cttactactt ttgtgcattt tgcatttgat aataaatacc
    10201 tttttgagct attaattcag agtggttacc cacttctaca atttcaccag ataccataac
    10261 taaaataata tctgctgttt gaatggtaga taagcgatgt gcaataataa agcttgttct
    10321 ccccttcatc aacatttgga atgcttcttg aacaagaact tcagtacgag tatctataga
    10381 cgatgtcgcc tcatctaata tcaatattct aggtaattta agaaaaatac gagcaattgt
    10441 caatagctga cattgcccct gtgataacga atctccagca tcttctaaat acgtatcata
    10501 tccattcggt aattgtttga taaagaaatc tgcattagct gcttttgcag cctctatcac
    10561 ttcttcacgg ctagctttag gattggcata tgcaatgtta tcgtgaatcg tagcactttt
    10621 taaccacgtt tcttgtaata ccattccaat ttcttgcctt aattgtgatg gctcataatc
    10681 agtaataggc ttaccatcta aaaggatatt tcccccgtca acttcataga aacgcattaa
    10741 taaattgatt aaagtagact tcccagcacc tgttggtcct acaatagcga ccttagctcc
    10801 tgctggaatg tgtaaattaa taccattcaa tagtggtttt gatttattat aaccaaaaat
    10861 aacgttttta aaatcgattt gacctttgac cgttgatgaa tcaagttttt ctgtacctgt
    10921 tatatttggt gacgattctt ctaaaataga gtaaaggcgt tctgcacaag ccagagcact
    10981 ttgcatctca gacaggacgg aagagatgtc attaaatggc ttagtatatt gattgacata
    11041 atttaagaaa gtgattaatt gaccaacact aaaagcacct gacataatac gaagggcacc
    11101 aatccctgct aagaagccat aaatcaaact attgataaat cgggttgatg gattgacagt
    11161 tgaagaataa aaaattgctg attgagaaaa gttagcatac tcttgattaa tagtcctaaa
    11221 atgatcacta ctttcttctt gcgcactgaa tgcttgaata aggctttctt gactcaccat
    11281 ctcttcaatg aattgagttt gtcgtcctcg tgatgccgtt tgattttgat ataagtgata
    11341 actcttttta gcaataaagc gggctagaaa gagtgataaa ggcgtcagaa ataataccaa
    11401 aaaaagcatt aataaatcaa tctttgccat actaaaaata gtaactagaa tagttaataa
    11461 accaacaaag aactggttaa aaaccattaa taaaccattt gataattgct ccgtatcggt
    11521 agtcacacga ctaatcaagt cacctatgcc acgtttatca aggtaagcta taggtaatag
    11581 attcaacttt tccattacag cctttcttaa agaagcaaca taatgaaaaa ttaagcggtt
    11641 atagagcaga ggattaatcc attgaataat agtatttgct aaaataactg caaccatctt
    11701 ccacattata ggtaacaata atatcataga atgaggcgat aatacaacgt ctaccgcctg
    11761 tccaattaga acaggcaaat aaacagtgag gcaaacttgt actaccgtcc ctaataatgc
    11821 cattcctact aagctttttt tagaaagaag atcttgaaca agcttttttt gtatcttttt
    11881 aggatgactt gctttcattt actcctcctc cttaaaattt tgtgaatggt aaatggactt
    11941 ataaactttg ttatgagcta ataaagattg atgattatca aaaccaactt gtttgccctt
    12001 atcaaggagt aagattctat ctgcattttg tatactatta attctctgtg atacaataat
    12061 aagattagtt tggttgaaat gtttaattat agctttaaat aaacgagctt ctgttaaata
    12121 atccaatgca gatgttgcat cgtctaaaat caaaaatgga atcttatctt gaactaaagc
    12181 tcttgcaatc gtcaaccttt gcctttgacc accagagaaa ttcctaccaa aactctctac
    12241 aggggcatca agttgaccat ctttttcttt tacaaaatca ctagcttgtg ctatttctaa
    12301 agcagtccaa agtttttcct cactaacttt accaagtccc aaagaaaggt tagaacgaat
    12361 agttccttta aacaactgag cattttgagg aacaactctt accaggctac gccaatttga
    12421 aattgtatct ggactttttc cctgatgata tatatcaata tccccttctt gcactttata
    12481 aatatgaagc agtaaattaa ccaaggttga tttcccagag ccagttccac caattatccc
    12541 taaagtttct cctacattca tagaaaaagt aatatcagat aaagcttttt cagaggagtt
    12601 tggataagaa aatgccattt cttgcacttc taaagcctta ttcgaatatt ttggctcaag
    12661 tttgtcatca attatttcag acggtctttc aaaaacggct ataattcgct tagcactgat
    12721 ataactttgg ttcagtgatg tcaccaacat tgtcattttt aacaattcaa caagaatttg
    12781 caataagtaa ttaattaaag caaccaacat tccttgtgat aaaagatgat tagctatatt
    12841 taaattacca cgccaaataa tcacaaccaa agtgatatta acaactagaa atgttagagg
    12901 cgttactaaa ctagaaagcc tacctgcttt taattgtaaa ttcgtatagt gataattgat
    12961 atcattaaat gcttcactct ctctatctac ttgattaaag gcacggataa ctcgtacacc
    13021 ttgaagttgt tgcctagtca actttaccaa ataatcggtt gaagttctga ttttaaggta
    13081 aataggattt aatagtcgag acattacaaa aacaatgata aataacgtga caaccattat
    13141 caagaaccaa atagtcaatg agggactaat ggaaaaagcc ataattatgg caccaaaaac
    13201 aataatcgga gccctcaaaa ataaacgtaa aaattgattt aaaccagttt ggatgtgaaa
    13261 tgtatcagca gtcaaacgag taatcaaact agcagttccc aattcatctc tgtctttttt
    13321 acccagtgac atgacttttt gatagagatc ctctgtcaat tgtctcgtat aaccaactgc
    13381 ggcttttgaa gaataatatt gagcagttat cgctactaca acacccaccg cagccaaaaa
    13441 gaaaatcaat ccaatttgca acagtaaact acttctatcc cctcgtggaa tggtctcgtc
    13501 aatcattttt gcaataacga ttggtaccaa aagttcaaaa gaggcttcta ataatttgaa
    13561 aaggggtcca aagatagttt ctttgatata ccccttaaaa taataagtta gtcttttcat
    13621 acttaccctt cttaaaacaa ctccattatt tttatcaaat aaaggttgaa ttgaacttca
    13681 cgtgagtagt aaatattacc accattttta tagagcttcc ctcctgaaat taaggcgaga
    13741 ggatgatagt aaaagtaagt gtccccgtta taatttccta ggctgggagc tacaacattt
    13801 ttagagtaat ttttggccaa ctcaatggaa ttttcacccc catgatccgc tacataatca
    13861 atataagaag aaccaaaatt atatgcttgg acagcagtcc atacatcaac cttagctttt
    13921 ttagcttgac ttatattttg tgacaacaga gtgacgccat gtttaatact ggtgtgacta
    13981 tccgtaatac tgttcgtagt tccactgata ctttcacttg attgcatcac atctatagca
    14041 tctcccttag tttcagtata aatcatagat aatactaagt gcacattagc ctctgaacca
    14101 ttctctgcaa gagtattctg cactaaatca tgatattgaa gcactttttt aacattttca
    14161 tggatgataa ctagacggta cccaatgaaa ataataataa ttaatgctat caaacgtttt
    14221 aaaaatttaa acatattatt tattttgaat atcctgtatg tttttgatta gtattgagta
    14281 tgtaccatca gggttttgga taaattcaac tgattcggca tcttggtaga cattgttggg
    14341 tacaatcaat tcaataccat ttgaaagaga tagcttctga ttctctaact tcttaatttg
    14401 acgggaatga tcaatatcac ttacttgaac agatccaggt attgcgtctt tcaattcatc
    14461 aacaaaagtc agcctggctg ttaaattatc atcaaataat tgatctgcta atttttctgg
    14521 agacaattct tgctcttctt ctaaattctt ataaatagcc gatttcattt ttgattggaa
    14581 agaaaaatca tctttgttga aattttcagc aattttttga gctgtctgtt caaccatttt
    14641 tatagatttt ttaacagact gttctggttg tacttgtaat aaattttcag aaaaataatt
    14701 agcaaatgaa ccattgtgtt tgatacgctt ttcaattaaa tagtattgct ttgatgactt
    14761 gttaacaacc aaagcttcat ctggagtttg agcagcactt ggaaggttat tctgtgttat
    14821 tgtaattggc tgctcttgat tttcagaaac atgagcaaat tgttccttta atgagattcg
    14881 aaggaaagca aaatgttcca ttccatcctt gtcaaattga ataaaaacta agtcattcgt
    14941 tttttgatct tccgatatga caaactcttc tttccaaagt tgcgcaattt gacaggaaga
    15001 aacatataaa tcatcttgta aatgactcat aaatacatta tcctctccaa aatagccacg
    15061 cttagcttca tcagaaaaaa ctttacttaa ttttttacga aagtagtcat caatccttgg
    15121 agttagggtt aacggagtat ctgatagcac taactctgtg tcgttaggag aaaactgatg
    15181 tataataatc tgtttgatat aaaaatccat cataacatta attctcatac aaagggaaag
    15241 cgtccgttaa tgatttaatt tcttgacgaa cttctgtgag gacatcttga ttttcatgat
    15301 ttttgagtgc cttaatcatt aattcagcga ttcttcgaga ttcttcaact cccattcctc
    15361 gactagtaat tgctggagta ccaattcgaa tacctgatgt tttaaatggt gatagtcgct
    15421 caaacggtat agagttcttg ttaagggtga tattgacttc ttcaagaaca ttttgtgcct
    15481 ttttgccatt ctcaataact tttgtgacgt ctactaagaa caaatgatta tcagtaccat
    15541 cagaaatcaa atgaaagtca tcatcttctt taaaaacttt agccatggct tgagcatttt
    15601 taataatatc ctcaccgtat attttaaagg aaggatctag agcttctttc aaagctacag
    15661 cttttgcagc aattacatgt tctaatggtc cgccttggag tcctggaaag actgcagaat
    15721 taattttttt agcaattgct tcatcattgg ttaaaatcaa accacctcta ggtccacgta
    15781 gagtcttatg tgttgtcgtt gtcgttacat gagcataagg aataggacta ggatgatgac
    15841 cagaagcaac taaacctgca atgtgagcca tgtcaaccat taagtaagca tctactgcgt
    15901 ctgctatttg acgaaacttt tcaaaatcaa taatgcgaga ataggccgac gcccctgcaa
    15961 caattaattt aggttgcgtt tcctgtgcta ttttcaaaat gttatcatag tctagcatct
    16021 ccgtcttagg atcaacagag taagaaacaa aatgataagt ttttcctgag aagctaactg
    16081 aagcgccatg tgttaagtgc ccacctgctg ctaagtccat ccctaagaca gtatctcctg
    16141 gctcgattaa agccatgtaa gctgctgcat tagcctgact accagaatgt ggttgaacat
    16201 tagcaaactc agcattaaat aacatcttag ctcgttctat agctaaactt tcaactacat
    16261 caacacaatc tgttcctcca taataacgat gactaggata cccttcggca tatttatttg
    16321 tcaaaacaga accttgtgct gccataacag cttttgaaac aacgttttct gatgcaatta
    16381 attcaatatt attttgttgg cgaatttcct catcatgaat agcctgccaa agttcttggt
    16441 caaattcttt aaaattgtct ttatcaaaaa tcatttttta ctccttaagc tcgttataac
    16501 ttttaaataa tggtttcaat cattatattt atcacatttt tactattagt tgcaagatga
    16561 ttaaattaat tttcaaaaat taattcaggt atcgtagccg ttagtacctc ttgcgtgatt
    16621 gcaccttgcc ttaaaatctt aactctatca ccagacaaat caataatagt agaatccaca
    16681 cctgtcaatg ctttatcatc ttcaatacct aaaacttgat gattaaactg tttttgaatt
    16741 tctgagaata cacgaccact atcacatcca gaaatattag cagaaggacc aatcaatgga
    16801 ccatggttag caatcatatc aagggttaca gggtgcttag gcatccgaaa tcctacgctt
    16861 gataacccag aattgatcca atccggaact tcttgtgaag cttccaaaat aatggttagc
    16921 ggtcctggta aaaaagcatc gtacaattgc ttcaaatagg tcggttgatt tttagaatat
    16981 tttaaaatag tctcaaaatc acatatattt aagttcatag ccttatcacg aggtcttttt
    17041 ttaagctcat agacgtggtt cacagcttct tcactcaaag ctttggcaaa gattccgtag
    17101 accgtttctg ttggcataat aactgcgcca tgatcctcta aaatctgtcc caaatcattc
    17161 attatctaat accaccattc tatctttacc aaatatatct ttcaaaactc tgcaacgatt
    17221 gtttggaaaa tatcttttca ataaacttcg taaatcatca ccttgtttgt aacctatttc
    17281 aaaatataat ttacctctag gttgcaaata ttctcttgag ttttcaatga ttttacgata
    17341 aatagctaat ccttcttcgt ctgcaaaaag ggcactatga ggctctgaag ctagcacatt
    17401 cttccccacc tcgtctttgt cattgtaaga aatataggga ggattagaca caataatatc
    17461 aaatttatct gaaatgttac taaagacatc tgattctata aaagtcactt ccgcatcaca
    17521 attattagca ttttcctttg ccaagtctaa tgcagataca gaaatgtcac tagctagaac
    17581 atcccaagag gggcgttctt tttttagaga aatggcaatt gctccactac ctgtaccaat
    17641 atctaaaaca gaacaatctt ctactttatt ctctgataag attagatcaa ccaactcctc
    17701 tgtctcaggc ctcggaatta aaactcgttc atcaacaaag aaaataagat ctctaaagta
    17761 agctttacct gtaatatact gcggtgatct atgtttttta agctgttgaa aaatattttc
    17821 cattaattgc tgatcagtat cactcacact actattttga tttaaaaggt aatcagttgt
    17881 tgaccattgt tttaggtctt ttaatacata aataaaattt tcgacttctt caccacaagc
    17941 ctctaacaac tgttcataat gcttgattaa ctgcgcataa ttcattattt atttaaggct
    18001 tctaactttt gagtctgatc atacataaca agtgcatcaa taacttcatc catttttcca
    18061 gataaaatag tatctaattt ttgaagtgtc aaaccaatac gatgatctgt tacacgattt
    18121 tgtgggaaat tataagtgcg aatacgttct gaacgatcac cagtaccaac tgttgattta
    18181 cgttcagcat cttgttcatc ttgagcaatt tgcgcaaaat ggtcagcaac acgagcacga
    18241 atgattttca tcgctttatc acggtttttt tgctgagtac gctcttcttg catttcaact
    18301 ttgattcctg taggaatgtg taccatacga actgcagtag caactttatt gacgttctga
    18361 ccaccagcac cagacgcgtg gtaaatatcg acgcgaagat ctttttgatc aatttcgtat
    18421 tctacttctt caacttctgg cattaccaaa acagttgcag ttgaagtatg aacacgaccc
    18481 tgactctctg tcaccggaac acgttgaaca cgatgagctc ctgattcata tttaagctta
    18541 gaataaactg actgaccaga aaccatagca accacttctt taataccacc aacaccatta
    18601 acggatgatt ccataacttc aaaacgccat ccttgagttt cagcatattt ttgatacatt
    18661 gttaaaagat caccagcaaa aagcgctgct tcatcgccac cagcagcacc acgaatttct
    18721 aaaataatgt ttttatcatc gttaggatct ttaggtaata gaaggatttt taatctttct
    18781 tcatactctt ctttagctgc ttttgattct ttaagttctt ctttagccat ttcttccaat
    18841 tcagcatcac cagaagcatc cttgatcatt tcttcagcat ctgaaatatt ttgaatgact
    18901 tgcttatatt ctctataagc tgtgacagtt tctcgagtat tagcttcttc acgtgataat
    18961 tccataaatc gctttgtatc tgaaacaaca tctggatcac taagtaactc acctaactct
    19021 tcataacgat cttcaactgc ctgcaattga tcataaatat tcatttttct ttgtatctcc
    19081 tttaaaaccg attatctttt aaatatctgg attaaaataa tgttttcgac aaactgggat
    19141 atatgtttca ttaccaccaa tttgtatctg atcaccttca tagacaggtt ttccgttttc
    19201 tgtccttaaa accatggttg ctttcttact acagtattgg caaattgttt tgatttcatc
    19261 aatcttatcg gccaataata gcaaatgttt tgaaccttca aaaagattgt tttggaaatc
    19321 atttttcaaa ccaaaagcca tgactggtac atctaagtca tctactactc tagctaaatc
    19381 gtagacgttt ttcttactca aaaattgaca ttcatcaatt aaaacacaat aaggcttttg
    19441 tggtaaattc tgaatatagg agaaaatgtc catatcatct gaaattggaa ctgcttcacg
    19501 acgcattcca atacgacttg aaacgactcc aaattcatct cgagtgtcca aggcgcttgt
    19561 cataataacc acaggttttc cttgttcttc atagttatga gcaactttta aaatttcaat
    19621 ggttttacca gaattcattg tcccatattt ataatagagt tgagccaaag cggaaaatcc
    19681 tactttctat agtattctac ctattttacc acaaaggctg aagttttccc agccattgaa
    19741 aacagtgtgt actttttcac aacattatgt tacaatgatg cccaataaca taaaggagta
    19801 atattatgcc atttgtaaaa attgacttgt ttgagggacg ctcacaagaa caaaaaaatg
    19861 aattagctcg cgaggttact gaagtagtat cacgcattgc aaaagcacct aaagaaaata
    19921 tccatgtttt tatcaacgat atgcctgaag gaacctacta tcctcaaggt gaacttaaga
    19981 aaaaataaga aatagtaaaa gaggttaccc aggtaacctc ttttgatagt ctttgcaagc
    20041 atatttcttg acaaattgaa aatatttgaa aataagtatt gattttcaag ttagaaaacg
    20101 gtatcattag aatatattgc tttgggggtg gaaaatttgc aactttctca tagtttaaga
    20161 ctcatgggaa cgactatcga tattcaaatt aattctaaaa atgctcaaaa acaaatccaa
    20221 gaggtcatag aactcttgga gctctacaaa aatcgcttta gtgcaaatga ctttaattca
    20281 gagttaatgg ctataaataa caacgctggt atcaagccta ttcaagtaca tccagacctc
    20341 tttgaattaa tcacaattgg gaaagaacac agcttagcta ggcctagtaa cctaaatatt
    20401 gctattggtc ctttagtgca aacttggaga attggatttt cagatgctaa actacctagc
    20461 cctagtgaag tatcagaagc tatgatttta tctgacccaa ctcatatact tctagattca
    20521 aaaaatcaat ctgttttttt aaatcagata ggaatgaaaa ttgatttggg tgcacttgca
    20581 aaaggatata ttgctgacaa aattatgacc tatctcaaaa atgagatgat tgattctgct
    20641 atcattaatt taggcggaaa cgttttagtt cacggagata atcctaatcg ttcagaaggg
    20701 tattgggtta tcggcattca acatccgaaa aagaaacggg gacaaaatat tggagctgtc
    20761 aaaattaaaa accaatctgt tgtaacctca ggtacttatg aacgccgtct gattattgac
    20821 gataaggaat accatcatat ttttgatcga cagactggtt atccgataca aacagagatg
    20881 gctagcattt ctattgtttc taaacaatcc gtcgactgtg aaatctggac cacaaggttg
    20941 tttggcttat ccattaaaga ggctcttgat attttaaacg ctgtttccta cattgaaggc
    21001 attatcatca ctaaagatga ccgtatctat ttatctgatg gcttaaaaca tcattttcaa
    21061 ctattttatc attaagaaag aggatttcta atatgaaact tattggcatc gttggaacta
    21121 actcaaataa atcaacaaat cgtcaattat tacaatacat gcaacaacac tttgctgaca
    21181 aagcagaaat tgaactcatc gaagttaaag accttccgtt atttaataaa cctgctgata
    21241 aaaatgttcc ccaagtaatt ttagatattg ctgctaaaat tgaagaagct gatggtgtta
    21301 ttattggtac gccagaatat gatcattcta taccttcggc cttaatgagt gttttagctt
    21361 ggctatcata tggtatttac ccacttctca acaaacccgt tatgattact ggtgcttcat
    21421 atggaacact tggatcatca cgtgcacaat tacaattacg tcaaatactt aacgctcctg
    21481 aactaaaagc aagtgtttta cctgacgaat tcttgttatc acactctctc caagcctttg
    21541 ataaagatgg caacctacac gatatcgaaa caagtcagaa gctagatgct atctttgatg
    21601 attttcgtct ttttgttaag attactggta aattatcaaa tgctcgtgat ttacttcaaa
    21661 aagaagcaga aaattttgac tgggaaagtt tataagagta tagaagggaa taactaagat
    21721 gaaatttgta ggtattgttg gctcaaatgc agagcaatca tataatcgta tgttgcttga
    21781 atttattcgc aagaatttta aaaccaaatt tgaattggaa gtccttgaaa tcgatgatat
    21841 tccaatgttt aatcaagtcc aaaattggga agaaagtttc caacttcgtc ttcttaataa
    21901 taagatcaca cgagctgatg gcgttattat tgccactcct gaacataatc atactattac
    21961 ggccgctctt aaaagtgtgc ttgagtggct ctcatttgct gtgcatccac tcgaaaataa
    22021 acctgttatg attgtcggtg cttcatatta tgatcaaggc acatcacgtg ctcaaataca
    22081 ccttcgtaaa atccttgatg ctcctggcgt taatgcttac acacttccag gaaatgaatt
    22141 cttattagga aaagctaaag aagcctttga tgataatgga aacattatca atcctgggac
    22201 tgttcaattt ttagaaactt gtcttgataa ttttatcaaa tatgtaggag ttgtatcatc
    22261 attgagaaaa cctaaagcta ttgaatctga agacctctac tgtcagggac aaatcgatac
    22321 aaccattact agtatcgatc cagatgatcc tgattggtta gacaaggcag ccgagaaagt
    22381 taacgccgtt gaaggggatg cttacgttaa actagacaat ggtttactta ctgttaacca
    22441 acttaatatg tttttaaatg ctatgccatt tgagctaaca tatgcagatg ataataatca
    22501 atttctatat tacaataata atcatccaga tccagataca atgttaggaa aacgtgttcg
    22561 tgaacaagta ggaaaccgta tggccacagt tcatggtagt ttaccaccgg cacgaatgaa
    22621 aaatgttgaa tgggttatcg gtactcttcg taatggaaat caagaatatg tacgtacagt
    22681 cgttcctggt acacctccag aagttatcaa tactcacaac tatcaagcta tgtactaccc
    22741 agacggatct tatgctggta ttaatgaaat tatctttaac tttaaaccgt ggttagattg
    22801 gtacctccaa actactggac aacgtttggt cggtggaact actgctgctc ctggagcttc
    22861 agcagttgac gctacttcta gcgcttcaac tagcacagag gatgctcccg ttgatcaagc
    22921 cacagatgcc acttctggtg cctctgaaca ttaatctcct cgcttttgag gatctgatac
    22981 cttaaaaaga tatcaagatc ctccttttta atatttaatc aagttaataa aatcacataa
    23041 aaggaatata ctatgtcact ttttcaagaa aaaattgctt acaattgcgc taaaaaggaa
    23101 gcgctttata aagagagttt aggacgctac gccttgagat caatgctagc aggggcttat
    23161 ttgacaatga gtactgctgc cggtatcgtc gcagctgata ctattggtaa aatttctcct
    23221 gctctatcag gttttgtatt tgctttcatc tttagttttg gacttattta tgttttaata
    23281 tttaatggtg aattggcgac atctaatatg ctttatctca ctgcaggagc ctataataaa
    23341 aatatctctt ggaaaaaagc cataacaatt ttaatttatt gtactttttt caacctcgtt
    23401 ggtgcttgta tattagcttg gttgtttaat caatcgtact cattccagca tttaacaaat
    23461 gatagctttt tagggcatgt cgttgctaaa aagttaggaa aacctagttc aggagctttc
    23521 ctagaaggta ttattgctaa tatgtttgtg aatttagcta tccttgctta catgttatta
    23581 aaagaagaat cggcaaaaat gacggttatt ttatctgcta tttttatgtt tgtttttcta
    23641 tcaaacgaac acttaattgc taattttgca tcctttatgt tagctgcttt tagccatatc
    23701 gaacatatta aaggttttac tcttctaaac gttatccgcc aatggacact tgtatttttt
    23761 ggtaactgga taggaggggg cgttttcatc ggcctagcct atgcttggtt aaataaaaca
    23821 aaaacttctc accatgaata aaagaaaaac tgagtaattt attactcagt ttttctttta
    23881 tattctcatc atgaaatcaa taactaaaaa ttaacttttt tgataaataa aggaaagtat
    23941 catattaatt ttttaggcaa tccattcaaa atgatattta aaacaacagc tgtcagggtt
    24001 gagataacaa taccatttgt caaaaacatt tgtaaagtat taggaagact aatgaagaga
    24061 tttgtctcat taaaacctac tcctgctgca attgatacgg ctgcaatgat aaagttatgc
    24121 tcattatgct caaaatcaac ctgattaagc attttcatcc cttgaagtgc taccatacca
    24181 aataaaacta acatagcacc accaagaact ggacttggaa tcatttgagc cattgcgcca
    24241 aatttaggta acaagccaag tataactaag aagagagctg taaaatatat cggcttacgc
    24301 gtacgtatcc cagatatctg taccagtccc acattttgag aaaaaccagt atatggaaag
    24361 gtattaaata agccgctaag taatactgcc aatccttctg aacggtaacc attacgaagt
    24421 ctcttactgt ctaatttatc gtttgtaata tctgaaagcg caaggtaaac acctgttgat
    24481 tctaccatag aaactgttgc aataatacac atcattaaaa tagaagtaaa ttcaaatcta
    24541 ggagctccaa aataaaatgg cttcggaata tgtacaagtg gtgcttctgc taccacagaa
    24601 gcatcaacta agcccataaa tgctgcaaga attgtacctg aaatgagtcc aataaggatt
    24661 gaaattgact tcaaaaagcc ttttgcgaaa atattaatta ataaaacaac accaatcgtt
    24721 accagtgata aagttaaaga ttgtaagctt ggttcttttg cattatcacc catatttccc
    24781 atcgcaactg gtattaatgt caaaccaatt gtagtaataa ctgatcctgt tacaattgga
    24841 ggaaagaaat ttgcaacttt agaaaaaatg ccagcaacta aaacgacata tattcctgaa
    24901 gcaattaaag ctccaaacat atagccagaa ccttgttgtg caccaataat tgataaagga
    24961 gctacagact gaaaggcaca ccctaataca actggaagac ccactccaaa gtgcttactt
    25021 aatcgtaatt gtaataaggt ggcaatccca cacataaaaa tatctgtcgc aataagataa
    25081 gttagttgct tagcattata accaagagca ctagcaatca tgataggtac aagaatggat
    25141 cctgcataca tagctaacag gtgttgcaag cctagaagtg ctgcttgcga attactttgc
    25201 tcattaaatt tcattaagca tcagcctccg caaaaacaac tcgaccattt tcaaacgctt
    25261 taatacgagc aagagaagta accggaacac ctgttttttc taacaaatca cgcccatctt
    25321 ggaaagattt ttcaataacg attccgatac cagcaacctt agctccagct tgaccaataa
    25381 tttcaagtaa tcctttagcc gcttgaccgt ttgctaaaaa gtcatcaatg atgagtacag
    25441 tatcatcgtt agataaaaag cgactcacaa tagaaacttg actcgtaact tgctttgtaa
    25501 aagaatacac ttcagcagtt aagatacctt cagtcatagt aatgttctta gcttttttag
    25561 caaatatcat tggtacgccc aatgcttgag ctgcgtacac tgctggcgca attccagatg
    25621 cttcaattgt aacaaccttc gtaatgccgg cttctttata tttatcagca aaaactttac
    25681 ctatttcctg cattaactca aaatttacct gatgagtcaa aaaagaatca acttttaaaa
    25741 tgttctcacc taaaacatca ccatctttaa gaatacgttc ttccaataac ttcataataa
    25801 ccccttcact ctttagaaaa taaaaaaata acttacctca atcagaggac acaaatctcc
    25861 tctaatttat cgcttcatta acatgaacca acaggcaagc tatttattag cttacttatt
    25921 gttagacatt acggtgtctt gtagagacgc taagcc
//
LOCUS       DBJYSH010000011        25552 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.011, whole genome shotgun sequence.
ACCESSION   DBJYSH010000011 DBJYSH010000000
VERSION     DBJYSH010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 25552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25552
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.011"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            43..1671
                     /locus_tag="ACUBV3_000385"
     CDS             43..1671
                     /locus_tag="ACUBV3_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HHU3846921.1"
                     /translation="MTKYLKYISFVALFLASIFLVACQNQNSQTKERTRKQRPKDELV
                     VSMGAKLPHEFDPKDRYGIHNEGNITHSTLLKRSPELDIKGELAKKYKISKDGLTWSF
                     DLNDDFKFSNGEPVTADDVKFTYDMLKADGKAWDLTFIKNVEVVGKNQVNIHLTEAHS
                     TFTAQLTEIPIVPKKHYNDKYKSNPIGSGPYMVKEYKAGEQAIFVRNPYWHGKKPYFK
                     KWTWVLLDENTALAALESGDVDMIYATPELASKKVKGTRLLDIASNDVRGLSLPYVKK
                     GVVKNSPDGYPVGNDVTSDPAIRKALTIGLNRQKVLDTVLNGYGKPAYSIIDRTPFWN
                     PKTAIKDNKVAKAKQLLTKAGWKEQADGSRKKGNLKAEFDLYYPTNDQLRANLAVEVA
                     EQAKALGITIKLKASNWDEMATKSHDSALLYAGGRHHAQQFYESHYPSLAGKGWTNIT
                     FYNNPTVTKYLDKAMTSPDLDKANKYWKLAQWDGKTGASTLGDLPNVWLVSLNHTYIG
                     DKRINVGKQGVHSHGHDWSLLTNIAEWTWDESAK"
     gene            1784..2761
                     /locus_tag="ACUBV3_000386"
     CDS             1784..2761
                     /locus_tag="ACUBV3_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HHU3846922.1"
                     /translation="MKHITTVVIWKILRCLSLVFGVSILTFILLKQSPVDPVMASVNY
                     DTSLTPVQYKAIAHHYGLDKPAPVQYFIWLKNFIQGHLGTSLVYRQPVIDIIRSRAGA
                     SFILMGLSWVLSGLIGFILGTLSAFHQGKLLDRIVRWFSYLQISVPTFWIGLIFLLIF
                     SVQLGWFPIGISSPIGTLSQDITLADRIKHLILPVFTLSILGIANVTLHTRTKMMSVL
                     SSEYVLFARARGETEWQIFKNHCLRNAIVPAITLHFSYFGELFGGSVLAEQVFSYPGL
                     GSTLTEAGLKSDTPLLIAIVMIGTLFVFAGNLIADILNSIINPQLRRKV"
     gene            2758..3579
                     /locus_tag="ACUBV3_000387"
     CDS             2758..3579
                     /locus_tag="ACUBV3_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HHU3846923.1"
                     /translation="MILRRRTIVLWQLGIAISLILSILALNLYFHSTPLQTNAALRNL
                     APSLNHLFGTDGLGRDMFVRTIKGLYFSLQVGLLGALMGVILATVFGVLAGLGNSIID
                     KIIAWLVDLFIGMPHLIFMILISFVVGKGAQGVIIATAVTHWPSLARLIRNEVYHLKN
                     KEFVQLSKSMGKTPYYIVRHHILPLIASQIFIGFILLFPHVILHEASMTFLGFGLSAE
                     QPSVGIILSEAAKHISLGNWWLVIFPGLYLILVVNAFDTIGESLKKLFYPQTDHF"
     gene            3591..4394
                     /locus_tag="ACUBV3_000388"
     CDS             3591..4394
                     /locus_tag="ACUBV3_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HHU3846924.1"
                     /translation="MTETLLSIKDLSITFTQYGRFLKPFQSTPIQALNLEIKKGELLA
                     IIGASGSGKSLLAHAIMDILPKNASVTGDMIYRGQSLNSKRIKQLRGKDITLIPQSVN
                     YLDPSMKVKHQVRLGISENSKATQEGLFQQFGLKESDGDLYPFQLSGGMLRRVLFTTC
                     ISDKVSLIIADEPTPGLHPDALQMVLDQLRSFADKGISVIFITHDIVAASQIADRITI
                     FKEGKAIETAPASFFSGNGEQLQTEFARSLLRSLPQQEFLKGVTHDLRG"
     gene            4378..5004
                     /locus_tag="ACUBV3_000389"
     CDS             4378..5004
                     /locus_tag="ACUBV3_000389"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001932323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HHU3846925.1"
                     /translation="MTLEAKKLGFYHKKDQWLFKEINLEVAPGQVLGIFGQSGCGKTS
                     LSKVLAGFLHPRSGEVLVDGSNLPNKAFRPVQLIQQHPEKTMNPLWPMKKSLEEAYYP
                     SRDLLDAFGIQEKWLNRRPSELSGGELQRFSIVRSLHPETKYLIADEMTTMLDSITQA
                     SVWKSLLEIVKDRNLGLIVISHDFAMLEKLCNQCYMIEENRIVSFNGD"
     gene            5286..7316
                     /gene="treP"
                     /locus_tag="ACUBV3_000390"
     CDS             5286..7316
                     /gene="treP"
                     /locus_tag="ACUBV3_000390"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HHU3846926.1"
                     /translation="MEQFKHDAKALLEAIGGKENISAVTHCATRMRFVLNDSSKAKVK
                     VIEELPSVKGTFTNAGQFQVIIGNDVPIFYNAFVAVSGIEGVSKEAAKSAAQKNQNPL
                     QRVLTMLAEIFTPIIPAIIVGGLILGFRNILDAVPFEFLGQKVVDGVRQVDSSGHPIW
                     NTLVDVSTFWSGVDSFLWLPGEAIFHFLPVGIVWSVTRKMGTTQILGIVLGICLVSPQ
                     LLNAYSVASTSAADIAKNWSWNFGYFTVQKIGYQAQVIPALLAGLSLSYLEIFWRKHI
                     PEVVSMIFVPFLSLVPAIILAHTVLGPIGWTLGKWISAIVLIGLTGPVKWLFGAIFGA
                     LYAPFVIAGLHHMTNAIDTQLIADTKTHTTGLWPMIALSNIAQGSAVLAYYFMHRHDE
                     KEAQISLPAAISAYLGVTEPALFGVNVKYIYPFVAGMIGSSVAGLLATTFNVQANSIG
                     VGGLPGFLSINVKYMGYFFICMAVAIFIPLFLTLFFKKSGILTKTEEEKLVSDAVIAS
                     TTETKSAKEKAVVSGTKLSVVSPLSGLAKPLDQASDPVFSQGIMGKGVVIDPSDGELV
                     SPVDATVSVLFPTKHAIGLLTSEGVEFLIHIGMDTVNLEGKGFTSHVAQGDNVKVGDK
                     LITFDIPMIKEEGYIVETPILITNQEEFRPEELIDLPKQIKRGQALMVAKKI"
     gene            7538..9163
                     /gene="treC"
                     /locus_tag="ACUBV3_000391"
     CDS             7538..9163
                     /gene="treC"
                     /locus_tag="ACUBV3_000391"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HHU3846927.1"
                     /translation="MTIDKRKVVYQIYPKSYKDTTGNGVGDLRGIIEKLPYLAELGID
                     MVWLNPFYPSPQRDNGYDISDYTAINPDFGKMDDFEEMIEVGRQYRIDFMLDMVLNHC
                     SIEHEWFKKALAGDRYYQDFFILRDNPTDWVSKFGGNAWSPFGDTGKYYLHLFDITQA
                     DLNWRNADVRKELFKVVNFWRDKGVKGFRFDVINLIGKDEILENCPINDGKPAYTDRP
                     ITHDYLKMLNNASFGQADSFMTVGEMSSTTIANCILYTAPEREELSMAFNFHHLKVDY
                     KDGQKWTIMAFDFPALRDLFHSWGEGMSEGNGWNALFYNNHDQPRALNRFVDVKRFRN
                     EGATMLATSIHLSRGTPYIYMGEEIGMLDPDYSSMDDYVDIESLNAYQIMLDEGKSQE
                     EAFSIIRAKSRDNSRVPMQWDDSTNAGFSEGAPWLKVGKSYKEINVAKEKTGLIFTFY
                     QELIRLRKQLPIIADGNYKAAFKDNEKVYAFERHLDKQKLLVLNNFFAEKVKIKLPEN
                     YLQGQVLLSNYKDVTLDETVTLQPYQTLAILVS"
     gene            9383..11419
                     /locus_tag="ACUBV3_000392"
     CDS             9383..11419
                     /locus_tag="ACUBV3_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HHU3846928.1"
                     /translation="MVDNKTVVIMLVFLARKNLSLYELTVQTKFSIKVIIEQINYLNS
                     FLAKNHLPAIAHSAGRYQLLGDEKEHDKIVSLLEAEQFYLTQEERVCLIYLYSFCRRE
                     FVSNVHYQDFLKVSKNTTLSDIKMLRSKLAKRGISLTYTRAKGYSLVGDEMDKHQVAF
                     QMITQLLESPIGFWSLNYILSSWKFALSYEKLEKTVEYFYESFQLSPIQDRLEKSLYF
                     IILILCRYQRSVDRVLQGSPIVSEQLKELTTIIVTNLSQDISLSKPLDQKEKDYITLI
                     LSGCFEGEGTKDDDFFEALAKAIVDEMETVSLLNFSNKEELLQGLKRHIIPAYFRLKY
                     GLTGDSGYTQNIKEHYSDLFLLVKKALRPLEEQVGLIPDSEISYFVIHFGGYLRQSGD
                     TQSMSYKALILCPNGVSSSLVIKEKLRGLFPQIHFHRVSKIEQLKLIDNQTYDMVFST
                     IFVETKKPNYLVSLMMTAEQVQQLKELVISDFPKACLDDFQLDQLIATIKKYAHVHCE
                     EELKLALRTMVKQDIFRKDVRPLLHQLITEETYQTSSEQMNWKEAIRLAAKPLLASGK
                     ITESYPEAMIEKVEEFGPFINLGKGIAIPHARPEDGVNSVGMSMLVLEQPIYLLDDPK
                     QEIYLLLCIAAIDNETHLKALSHLTTILRDNNNVKALLASRGYQDIEMIIKQED"
     gene            11422..11706
                     /locus_tag="ACUBV3_000393"
     CDS             11422..11706
                     /locus_tag="ACUBV3_000393"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008089859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HHU3846929.1"
                     /translation="MLRIGTACGSGLGSSFMVQMNIESILKDLGVSDVEVEHYDLGGA
                     DPSAADVWIVGRDLEDSAGHLGDVRILNSIIDMDELRELVTGICQEKGLI"
     gene            11719..13074
                     /locus_tag="ACUBV3_000394"
     CDS             11719..13074
                     /locus_tag="ACUBV3_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HHU3846930.1"
                     /translation="MKGLLDFLVNIASTPAILVALIAIIGLVLQKKGVPDIVKGGIKT
                     FVGFLVVSGGAGIVQNSLNPFGKMFEHAFHLVGVVPNNEAIVAVALTKYGSATALIML
                     AGMIFNILIARFTKFKYIFLTGHHTLYMACMIAVIFAVAGFTSFSLILFGGLALGIIM
                     SVSPAFVQKYMIQLTGNDKVALGHFGSLGYWLSGFIGGIVGDKSKSTEDIKFPKSLSF
                     LRDSTVSITISMAIIYLIVAVFAGEAYIAKEISNGVNGLVYALQLAGQFAAGVFVILA
                     GVRLILGEIVPAFKGISEKLVPNSKPALDCPIVYPYAPNAVLIGFISSFVGGLVSMIV
                     MIVTGTTVILPGVVPHFFCGATAGVIGNASGGVRGATIGAFVQGILISFLPIFLMPVL
                     GGLGFKGSTFSDADFGLTGIILGALNHVGGAIAIVIGIVVILIGLFGISFVGKSTHKE
                     G"
     gene            13077..13934
                     /locus_tag="ACUBV3_000395"
     CDS             13077..13934
                     /locus_tag="ACUBV3_000395"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HHU3846931.1"
                     /translation="MTSPEERYSSLRRFATEIRLNTLETLNHLGFGHYGGSLSIVETL
                     AILYGDIMDINPEKFKESDRNYMVLSKGHAGPALYSTLYLKGFFDKTFLHSLNTNGTK
                     LPSHPDRNLTPGIDVTTGSLGQGISIATGIAYAQKIENSSYYTYTIVGDGELNEGQCW
                     EAIQFAAHHQLHHLIVFVDDNKKQLDGLTADICNPGDFVAKFEAFGFDAVRVKGDDIE
                     AIDKAIKTFQDSNSVKPKCIVLDSIKGQGVKELEELASNHHLRPDLQQKTMLERAVIS
                     LRESLEVVE"
     gene            13931..14860
                     /locus_tag="ACUBV3_000396"
     CDS             13931..14860
                     /locus_tag="ACUBV3_000396"
                     /EC_number="2.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HHU3846932.1"
                     /translation="MRCSTKEMRLVYRDFLLQANQENKQITVLEADLSSSMSTNVLAS
                     EFGKRYINLGIMEAEMVGLAAGLAIKGYKPYLHTFGPFASRRVFDQVFLSLGYSQLSA
                     TIIGSDAGVSAEMNGGTHMPFEELGLLRLIPKATIFEVSDDIQFEAILKQTLSIDGLK
                     YIRTIRKASTAVYEGCEDFSKGFIQLRQGKDIALVASGIMVSRAIEAADYLKELGIEA
                     SVIDLFKIKPLPEELKPLLIDQSIVTIENHNRIGGIGSALCEWLSMEKNTTVSRMGID
                     ERFGQVGQMKYLLEEYGLAVKDIVEHCKSIYKS"
     gene            14969..16228
                     /locus_tag="ACUBV3_000397"
     CDS             14969..16228
                     /locus_tag="ACUBV3_000397"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin reductase family protein"
                     /protein_id="HHU3846933.1"
                     /translation="MRAWKGIVLILSSIVVTLVAWQNAGLSEFVVPGLALTSLSLTFL
                     LSTKFRILESYFQGIENMYFYHKVMAVFSMILLLLHKIGLGQGGHGSEFAKTIGSAGL
                     YLFLSIVFVAYFGNFLKYEIWRFIHRFVYLVYILGLVHTFLILGDRILGNTLLSLIVL
                     GYAVIGVISGFYIIFLYSRMRFRRVGYVQKVTHLNHDTTEIEIAMKRPYRYDYGQFTF
                     LKIYQAGFESAAHPFSISGGHDRVIFLTVKASGDYTKSIYKQLKVGTKIALDRAYGHM
                     LFDKDKKEQVWIAGGIGITPFISFIRENSILTKRVDFFYTFSNQDNLIYQDMLESYAK
                     ANPNFKLHLNNSSLQGRLDFSQSVFEGQPTIFMCGPTSMTSTYAKVFRQKDAKSRLVY
                     EGFSFRDSWLSIFLLKTFDKVYSNLIK"
     gene            16316..16585
                     /gene="rpsO"
                     /locus_tag="ACUBV3_000398"
     CDS             16316..16585
                     /gene="rpsO"
                     /locus_tag="ACUBV3_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HHU3846934.1"
                     /translation="MAISKEKKNEIIAQYARHEGDTGSVEVQVAVLTWEINHLNDHIK
                     QHKKDHATYRGLMKKIGHRRNLLAYLRRTDVNRYRELIQSLGLRR"
     gene            16966..19095
                     /gene="pnp"
                     /locus_tag="ACUBV3_000399"
     CDS             16966..19095
                     /gene="pnp"
                     /locus_tag="ACUBV3_000399"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HHU3846935.1"
                     /translation="MSKQVFEMIFAGKKLVVETGQVAKQANGSVVVRYGDSTVLTAAV
                     MSKKMSTGDFFPLQVNYEEKMYAAGKFPGGFNKREGRPSTDATLTARLIDRPIRPMFA
                     EGFRNEVQVINTVLSFDENASAPMAAMFGSSLALSISDIPFNGPIAGVQVAYVDGNFI
                     INPTAQEQEASALELTVAGTKEAINMVESGAKELSEEIMLEALLKGHEAVCELIAFQE
                     EIVTAIGKEKAEVELLQVDPELQAEIIATHNIALQAAVQVEEKKAREAATEAVKEVVI
                     GEYEARYAEHEEYDRIMRDVAEILEQMEHAEVRRLITEDKVRPDGRRVDEIRPLDAVI
                     DFLPQVHGSGLFTRGQTQALSVLTLAPMGEAQIIDGLTPEYKKRFMHHYNFPQYSVGE
                     TGRYGAAGRREIGHGALGERALEQVLPSLEEFPYAIRLVAEVLESNGSSSQASICAGT
                     LALMAGGVPIKAPVAGIAMGLISDGTNYTVLTDIQGLEDHFGDMDFKVAGTREGITAL
                     QMDIKIEGITPQILEEALAQAKKARFEILDVLHGAIAEPRPQLAPTAPKIDMIKIDVD
                     KIKVVIGKGGETIDKIIAETGVKIDIDEEGNVSIFSSDQAAIDRTKDIIASLVREAKV
                     GEVYHAKVVRIEKFGAFVNLFDKTDALVHISEIAWTRTANVADVLEIGEEVDVKVIKI
                     DDKGRVDASMKALLPRPPKADNPKKES"
     gene            19097..19849
                     /locus_tag="ACUBV3_000400"
     CDS             19097..19849
                     /locus_tag="ACUBV3_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SseB family protein"
                     /protein_id="HHU3846936.1"
                     /translation="MTSTNELDIRLRAFINAPDNFLDSIGLVNALHHSTVWASKEPYA
                     IQVDGQEVVPVFTDITDLNHFKEEQESARDMFWESRRSLDVLDEAISHGLAGLVYNLK
                     KEGDFGNSTIFYCEDMVQFMNNYTTILNQLLNEDNIVADIMDKTYLVPAFVHPREEGS
                     FDRFFPTMSTPEGKSYVPVFSNLLSFEKWYNHNDFGGAFRKAQGVILAWTIDDIYKPR
                     NGENEIDDTFGVAINPFDEQQVLVDWSDVEND"
     gene            19858..20442
                     /gene="cysE"
                     /locus_tag="ACUBV3_000401"
     CDS             19858..20442
                     /gene="cysE"
                     /locus_tag="ACUBV3_000401"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000539954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HHU3846937.1"
                     /translation="MGWWKESIAIVKEQDPAARSSLEVILTYPGIKALAAHRLSHFLW
                     NHNFKLLARMHSQFWRFWTQIEIHPGATISEGVFIDHGSGLVIGETAIVEKGAMLYHG
                     VTLGGTGKDKGKRHPTIRKGALISAHSQIIGPIEVGENAKVGAAAVVLADVPADVTVV
                     GVPAKVVRVHGQKDDLQIRSIEHDREESYYSSKL"
     gene            20452..20634
                     /locus_tag="ACUBV3_000402"
     CDS             20452..20634
                     /locus_tag="ACUBV3_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3846938.1"
                     /translation="MKDYVTTQILSILGILAGACLSYLKFAQGNSLLGIIWAAFALMY
                     IVRLYKAYQKQKKEKP"
     gene            20631..21974
                     /gene="cysS"
                     /locus_tag="ACUBV3_000403"
     CDS             20631..21974
                     /gene="cysS"
                     /locus_tag="ACUBV3_000403"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000591125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HHU3846939.1"
                     /translation="MIKIYDTMTRSLQDFIPLNEGKVNMYVCGPTVYNYIHIGNARSV
                     VAFDTIRRYFEYCGYQVNYISNFTDVDDKIIKGAAEAGMDTKSFSDKFISAFMEDVAA
                     LGVKPATKNPRVIDYMDEIIDFVKVLVDKEFAYEANGDVYFRVSKSHHYAKLANKTLE
                     DLEIGASGRVDGEGEIKENPLDFALWKSAKSGEVSWESPWGKGRPGWHIECSVMATEI
                     LGDTIDIHGGGADLEFPHHTNEIAQSEAKTGKTFANYWMHNGFVNVDNEKMSKSLGNF
                     ITVHDMLKSVDGQVIRFFLATQQYRKPVNFTEKAVHDAEVNLKYLKNTFNLPIQENAN
                     DEELEQFVKAFQEAMDDDFNTANGITVIFEMAKWINSGHYTSKVKETLAELLEIFGIV
                     FQEEVLDADIESLIEQRQEARANRDFATADRIRDELAKQGIKLLDTKDGVRWTRD"
     gene            21967..22353
                     /locus_tag="ACUBV3_000404"
     CDS             21967..22353
                     /locus_tag="ACUBV3_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HHU3846940.1"
                     /translation="MIDVRLINGIALAFEGDAVYSLYIRRHLIMQGFTKPNQLHRKAT
                     QYVSANAQALLINAMLEENILTDEEQLIYKRGRNANSHTKAKNADIITYRMSTGFEAL
                     MGYLDMTGQIKRLETLIQWCIETIEK"
     gene            22456..23211
                     /gene="rlmB"
                     /locus_tag="ACUBV3_000405"
     CDS             22456..23211
                     /gene="rlmB"
                     /locus_tag="ACUBV3_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HHU3846941.1"
                     /translation="MTMKDKQFKEESSDLVYGLHAVTESLRANTGNKLYLQDDLRGKN
                     VDKVKALATEKKVSISWTPKKILSDMTNGGVHQGFVLKVSEFVYADLSEIMTKAENEE
                     NPLILILDGLTDPHNLGSILRTADATNVTGIIIPKHRSVGVTPVVSKTSTGAVEHVPI
                     ARVTNLSQTLDTLKDKDFWIFGTDMNGTPSHKWNTKGKLALVIGNEGKGISHNIKKQV
                     DEMITIPMNGHVQSLNASVAAAILMYEVFRNRL"
     gene            23208..23726
                     /locus_tag="ACUBV3_000406"
     CDS             23208..23726
                     /locus_tag="ACUBV3_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000716639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HHU3846942.1"
                     /translation="MKKHSILLVDGYNMIAFWKDTRQLFKSNRLEEAREVLLRKLNHY
                     AHFEHIDIICVFDAQYVPGVRQRYDQYKISVIFTEEDETADSYIERAAAELNQSVLNL
                     VSVATSDLNEQWTIFSQGALRVSARELEQRVATVKSDLDKMSSQIDLSTPKLRPWNDE
                     QLGKLKDFLDGM"
     gene            23819..24679
                     /locus_tag="ACUBV3_000407"
     CDS             23819..24679
                     /locus_tag="ACUBV3_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HHU3846943.1"
                     /translation="MTFKILTDSTSDLDEKWAQEHNVDIIGLTIELDGKTYETVGDEK
                     ITSDFLLERMQEGAKPTTSQINVGQFEEVFSTYAENDHALLYLALSSHLSGTYQSATI
                     AREMVLDKYPDAQIEIVDTMAASCGEGVLAMLATKERQEGKSLEEVKQKIESLLPKLN
                     TYFLVDDLNHLMRSGRLSKGAAIIGSVAKIKPLLKLDSEGKLVPFAKTRGRKKGIKEI
                     VTQATKTLSYSTLIIAYSGEKDSAQVMKEQLLADERIEEVIIRPLGPVISAHVGSGAL
                     ALFSLGEENR"
     gene            25217..25336
                     /locus_tag="ACUBV3_000408"
                     /pseudo
     CDS             25217..25336
                     /locus_tag="ACUBV3_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002278327.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
BASE COUNT         8057 a         4041 c         5257 g         8197 t
ORIGIN      
        1 tgtcaaaaat attatttatc aattaaaaaa ggaggtttaa tagtgactaa atatttgaag
       61 tacatctctt ttgttgcttt gtttctggcg agtatctttt tagtagcttg tcaaaatcaa
      121 aattcacaaa caaaggagcg aacacgaaaa caacgaccca aagatgaatt ggttgtttct
      181 atgggggcaa agcttcctca tgaattcgat ccaaaggacc gttatggaat ccataatgaa
      241 ggtaatatta ctcatagtac cctattgaaa cgttctcctg aactagatat aaaaggagag
      301 cttgctaaaa aatataaaat ctctaaggat ggcttaacgt ggtcgttcga cttaaatgat
      361 gattttaaat tctccaatgg tgagcctgtt actgctgacg atgttaagtt tacttatgat
      421 atgttgaaag cagatggaaa agcttgggat ttgaccttta ttaagaatgt tgaagtagtt
      481 gggaaaaacc aggtaaatat tcatttgact gaggcgcatt cgacatttac agcacagttg
      541 acagaaatcc caatcgtccc taaaaaacat tacaatgata agtataagag caatcctatc
      601 ggttcaggac cttacatggt aaaagaatat aaggctggag aacaagcaat cttcgttcga
      661 aatccatatt ggcacggtaa aaagccttat tttaaaaagt ggacttgggt attacttgat
      721 gaaaatacag cactagctgc tttagaatct ggtgatgttg acatgattta cgcaacgcca
      781 gagcttgcta gcaagaaagt caaaggaaca cgtcttctag atattgcttc aaatgatgtt
      841 cgtgggttat cgctacctta tgtgaagaaa ggtgttgtga aaaattcacc agacggttat
      901 ccagtaggaa atgatgtcac tagtgatcca gcaatcagaa aagccttgac tattggttta
      961 aatagacaaa aagttctgga tactgtttta aatggttatg gtaaaccagc ttattcaatt
     1021 attgatagaa caccattctg gaatccaaaa acagcaatta aagataataa agtagctaaa
     1081 gctaagcaac ttttgacaaa agctggatgg aaagaacaag cagacggtag ccgcaaaaaa
     1141 ggaaatctta aagcggaatt tgacctttac taccctacta atgatcaatt acgagcaaac
     1201 ttagccgttg aagtagcgga gcaagctaaa gccttaggta ttactattaa actcaaggct
     1261 agtaactggg atgaaatggc aactaagtca catgattcag ccttacttta tgcgggagga
     1321 cgtcatcatg cgcagcaatt ttatgaatca cattacccaa gtttagctgg taaaggttgg
     1381 accaatatta ctttttataa caatcctact gtgactaagt accttgacaa agcaatgaca
     1441 tctcccgacc ttgataaagc taacaaatat tggaagttag ctcagtggga tggcaaaaca
     1501 ggtgcttcca ctcttggaga tttaccaaat gtatggttgg tgagtcttaa ccatacttat
     1561 attggtgata aacgtatcaa tgtaggtaaa caaggtgtcc atagtcatgg tcatgattgg
     1621 tcattattga ctaacattgc tgaatggact tgggatgaat ctgctaagta actaatttag
     1681 ttaatgtagg ctactttaag tttggagtca gagaaaacaa tgtacacctt atggtgtgaa
     1741 tggtttcctc gactcctttt ttaacaatta aggagattta tctgtgaaac atattaccac
     1801 tgtagttatc tggaagatac ttaggtgttt gtcactcgtt tttggagtat caattttgac
     1861 ctttatttta ctaaaacaat caccggttga tccagtcatg gcaagtgtca attatgacac
     1921 gtcactaacc cctgttcagt acaaagcgat tgctcaccac tatggcttgg ataagccagc
     1981 tccggtccag tattttattt ggttaaaaaa ttttatacag ggacatttag ggacttcact
     2041 agtttatcgg caacctgtta ttgatattat tagatcacgg gcaggtgcct ctttcatact
     2101 tatgggactc tcctgggtct tatcgggtct tattggattt atcttaggaa ctttatcagc
     2161 tttccatcaa gggaaattgc ttgatcgaat tgtaaggtgg ttttcttacc ttcagatatc
     2221 agtaccaact ttttggattg ggctcatttt tttattaatc ttttctgtcc agctggggtg
     2281 gttcccgatt ggtatttctt ctccgatagg tactttgagt caagatatta cgttagctga
     2341 tcgtattaag caccttattt tacctgtttt cacgctaagt attctaggca ttgccaatgt
     2401 aactcttcat actagaacta aaatgatgtc ggtactttct agtgaatatg tcttatttgc
     2461 cagagcgcgt ggggaaacgg aatggcaaat ttttaaaaat cattgtctta gaaatgctat
     2521 cgtaccagct attacactgc atttttccta ttttggagaa ttgtttggag gatccgttct
     2581 tgctgagcaa gttttctcat atccaggact agggtctacc ctaactgaag caggacttaa
     2641 aagtgataca ccgctactta tagctattgt gatgataggg acattatttg tttttgcggg
     2701 caatcttatt gcggatattt taaatagcat aatcaatcca cagttaagga gaaaagtatg
     2761 atattgagac gtcgaactat tgttttatgg caactgggta tcgccatttc tctcattctt
     2821 agtattctag ccttaaatct ttatttccat agtactccct tgcaaaccaa tgcagcttta
     2881 cggaaccttg ctccttcatt aaaccatctt tttgggacag atggtttagg tagggatatg
     2941 tttgtcagaa cgattaaagg actttatttc tctctacaag tcggcttatt aggtgccctt
     3001 atgggggtca ttctggcgac agtttttgga gtgcttgcag gtttaggaaa tagcattatt
     3061 gataaaataa tagcatggtt agttgatttg tttattggta tgcctcattt gatttttatg
     3121 attctcattt cttttgttgt tgggaaaggt gctcaagggg tcatcattgc aacggctgtt
     3181 acacattggc cttctttagc aaggcttatc cgcaatgaag tctatcatct aaagaataaa
     3241 gaatttgtcc aactttctaa aagtatggga aaaacgcctt attatattgt gaggcatcat
     3301 atcctgcctt tgattgcttc tcaaattttc attggtttta tcctcttatt tccacatgtc
     3361 atcctacatg aagcatcaat gactttctta ggatttgggc tctctgccga acaaccttcg
     3421 gttggtatca ttctgtcaga ggcagctaag catatctctc ttggaaattg gtggttggtt
     3481 atctttccag gactttatct tattttggtt gtcaatgcat ttgatactat cggagaatct
     3541 ttaaagaaac tcttttaccc tcaaactgat catttttagg aggtagagat atgacagaaa
     3601 cattattaag cattaaagac ctctccatca ccttcactca atacggaaga tttttaaaac
     3661 catttcaatc aacaccgata caagcgctga atttagaaat taaaaaaggt gagttattag
     3721 ctattatagg tgctagtggt tcggggaaga gtttattagc acatgctatt atggatattc
     3781 ttcctaaaaa tgcatctgta acaggagata tgatttatcg tggtcaatca ctaaattcta
     3841 aacgcattaa acagttgcga ggaaaagata ttacgttgat tccacaatca gttaattatt
     3901 tagatccatc tatgaaagtc aaacatcagg tgcgcttagg tatctcagaa aattcaaagg
     3961 ctactcaaga aggattgttt caacagtttg gtttaaaaga aagtgatggt gacttgtatc
     4021 ctttccaact ttctggcgga atgctccgac gtgttttgtt tacaacgtgt attagtgata
     4081 aggtttcttt gattattgca gatgagccca cccctggatt acatccagat gctctgcaaa
     4141 tggttttaga ccaactacgc tcctttgcag ataaaggaat aagcgttata tttatcactc
     4201 atgatattgt agcagctagt caaattgctg atcgtattac tatttttaaa gagggaaaag
     4261 ctattgaaac agctccagct agtttcttta gcggaaatgg agagcagtta caaacagaat
     4321 ttgctagaag tttattgcgc tctctcccac agcaagaatt tttgaaagga gttactcatg
     4381 accttagagg ctaaaaagct tggcttttat cataaaaaag atcaatggct ttttaaggag
     4441 attaatttag aggtagcacc tggtcaagtt ttaggtatat ttggacaaag tggttgtgga
     4501 aaaactagtt tatccaaagt gcttgcaggt tttttacatc ctagatctgg tgaagtatta
     4561 gttgatggca gtaatttgcc taacaaagca tttagacctg tacaacttat tcaacaacat
     4621 cctgaaaaaa cgatgaatcc tttatggcct atgaaaaaaa gtttagaaga agcttattac
     4681 ccaagtcgag atttactaga tgcttttgga attcaagaaa aatggctaaa tcgtcgccct
     4741 agtgaactct cgggagggga attacaacgc ttttcgattg tgcgttcatt acatccagag
     4801 actaaatacc ttattgcaga tgaaatgact actatgttgg atagtattac acaagctagt
     4861 gtatggaaaa gcctgttgga gattgtaaag gatagaaatt taggtctcat cgttattagt
     4921 catgatttcg ctatgttaga gaaactttgt aaccaatgct atatgattga agagaatcgt
     4981 atcgtatctt tcaatggaga ttaaaaaact cacctactat atcaattagt aggtgttttt
     5041 tattaagaga gtattggggg tgactgacct actcatgact ctaacatttt ggtaacgttg
     5101 gtgagaaatt ctaatgtttt gggtattatt gactaattat tttataattt atagattttt
     5161 taaaaagata agctaaggat ttgcataggg atatgtaatc gtttacaatt atattgtaag
     5221 ctagtcttct ttaagtttct gccttggagt cacagactct ccgttaaata gataaggata
     5281 aacagatgga acaatttaaa catgatgcca aagccttgtt agaggcaatt gggggtaaag
     5341 aaaatattag tgcagtgaca cattgtgcca cacgcatgag atttgttttg aatgacagta
     5401 gtaaagctaa ggtaaaggta attgaggagt taccatcagt caagggtacc tttaccaatg
     5461 ctgggcaatt tcaagtcatt attggtaatg atgtccctat tttttataat gcttttgtag
     5521 ccgtttctgg tattgagggt gtgtctaaag aggcggcaaa atcggctgcc caaaaaaatc
     5581 aaaacccttt acagcgtgtg ttgacaatgc tggctgaaat atttacaccg attattccag
     5641 ccattattgt tggtggtctg atccttggtt ttcgtaatat tttagatgcc gttccatttg
     5701 aatttttagg tcaaaaagtt gttgatggtg tcaggcaagt agactcttct gggcatccaa
     5761 tttggaatac tctagtagat gtttcaacgt tttggagcgg agtagactct tttttatggt
     5821 taccaggtga agctatattt catttcttac cagttggtat tgtatggtct gtgactcgta
     5881 agatggggac tactcaaatc ctcggtattg ttctaggtat ctgtctagtg tcgccacaat
     5941 tacttaatgc ttattctgta gctagtactt cagcagcaga cattgctaaa aattggtctt
     6001 ggaattttgg ttactttaca gtacaaaaaa ttggttatca agctcaagtt attccagctc
     6061 tgttagctgg tttgagcctg tcttacttag aaatcttctg gagaaaacat attcctgaag
     6121 ttgtttcaat gatttttgtg ccatttctat ccttagttcc agctattatc ttggctcata
     6181 ctgtccttgg acctattggt tggacactag gtaaatggat ttctgctatc gttcttattg
     6241 gattgactgg tcctgtaaaa tggctctttg gtgctatttt tggtgccctg tatgccccat
     6301 ttgttattgc tggtttgcac cacatgacaa atgctatcga tactcaactc atagcagata
     6361 caaaaacaca tacaactggc ctttggccga tgattgctct ttcaaatatt gctcagggtt
     6421 cagctgtact agcttattat tttatgcatc gtcatgatga aaaagaggct caaatatcac
     6481 ttccagctgc tatctcagcc taccttggtg ttacagagcc ggcgcttttt ggtgtaaacg
     6541 ttaaatacat ttatccattt gttgctggta tgattggttc aagtgtggca ggtcttttgg
     6601 cgacaacttt taatgtccaa gctaactcta ttggtgtagg cggtcttcca ggcttcctgt
     6661 caattaatgt taaatatatg ggatatttct ttatctgtat ggcagtggct atctttattc
     6721 ctttattttt gacactattc tttaaaaaat caggtatttt gacaaaaact gaagaggaaa
     6781 aattagtatc tgatgctgtc attgcttcaa caacagaaac aaaatcagca aaagaaaaag
     6841 ctgtcgtctc aggtacaaag cttagtgtag taagcccttt atcaggttta gctaaaccac
     6901 tggatcaagc tagtgatcct gtcttttcac aaggaattat ggggaaggga gtggttatcg
     6961 atccaagtga tggagaacta gtttcaccgg tcgatgcgac agtttcagtt cttttcccta
     7021 cgaaacatgc tattggatta ttaacatcag agggtgttga gtttcttatt catattggta
     7081 tggatactgt taatttagaa ggaaaagggt ttacctctca tgttgcccaa ggtgacaatg
     7141 taaaagtagg agataaactc attactttcg atattccaat gataaaagaa gaaggataca
     7201 ttgtggaaac tcctattctg attactaatc aagaagaatt ccgaccagaa gaattgatag
     7261 atttacccaa acagataaaa cgtggacaag cattaatggt tgccaagaaa atttaaaagt
     7321 aattaggtat cctttttaaa aaagagcact cttataaaat gagaggtaat ttgaggaatt
     7381 gtttgagggg cttaatacat tagctccttg aattattttg aacataagaa aaaactcatg
     7441 ggaacaaata tcgctgtaaa aatgttatat ttttgatgat agttgatgtt atagttagac
     7501 tgctaaatac agcagcattt tgggaaagga gtagaatatg acgattgata aacgtaaagt
     7561 tgtctatcaa atttatccaa aatcatacaa agacacaact ggtaatgggg ttggtgatct
     7621 tcgtggtatt atcgaaaaat taccctattt agcagaactt ggtattgata tggtttggct
     7681 caacccattt tatccgagtc cacaacgaga taatggatat gatatctctg attatacagc
     7741 cattaaccct gattttggaa agatggacga ttttgaagaa atgattgagg tgggacgtca
     7801 ataccgtata gattttatgc ttgatatggt gcttaatcat tgttcaatag aacatgagtg
     7861 gtttaaaaaa gcacttgcag gagatcgcta ctatcaagat ttctttatct tacgtgataa
     7921 tccaacagat tgggtatcta aatttggtgg aaatgcttgg tcaccatttg gtgatacagg
     7981 gaagtattat cttcatcttt ttgatatcac tcaagctgac cttaattggc gcaatgccga
     8041 tgttcgcaag gaacttttta aagttgtcaa tttttggcgt gataaagggg ttaaaggttt
     8101 tcgatttgat gtgattaatt taattgggaa agatgagata cttgagaact gcccaattaa
     8161 tgatggaaaa ccagcttata ctgatcgtcc aataacacat gattatctca agatgttgaa
     8221 taatgctagt tttggacaag ctgattcctt catgacagtt ggtgaaatgt cttcaacgac
     8281 aattgcaaat tgcattcttt acactgcacc agaacgtgaa gagttatcca tggcttttaa
     8341 ttttcaccac ctcaaagtag attataaaga tgggcaaaag tggaccatta tggcttttga
     8401 ttttcctgca ttacgtgacc ttttccatag ttggggcgaa ggaatgagcg aaggaaatgg
     8461 ttggaatgct ttattctata ataatcatga ccaaccgcga gcattaaatc gttttgtaga
     8521 cgtgaaacgt tttcgaaatg aaggagcaac catgttagca acatctatcc acttatcgcg
     8581 tggtactcct tatatatata tgggtgaaga aatcggaatg cttgatcccg attacagttc
     8641 tatggatgat tatgtggata ttgagagttt gaatgcctac caaataatgc ttgatgaagg
     8701 caagtcacaa gaagaggcat ttagcattat tcgagcgaaa tctcgagaca attctcgagt
     8761 accaatgcaa tgggatgata gtacgaatgc aggtttttct gaaggcgctc catggttgaa
     8821 agtaggaaaa tcatataaag aaatcaatgt cgcaaaggag aaaacagggc tgatatttac
     8881 tttctatcaa gagttgataa ggctacgtaa acaactgcca ataatagctg acgggaatta
     8941 taaagcagct ttcaaagata acgaaaaagt atatgctttt gaacgccatc ttgataagca
     9001 aaaacttctt gttttaaata atttctttgc tgagaaagtt aagataaaat taccggagaa
     9061 ttatctccaa ggacaagttc ttctatcaaa ttataaggat gttactcttg atgaaacggt
     9121 tactttgcaa ccttatcaaa ctttagctat cttagtgtca taacatttaa aaaaccttta
     9181 cttattcaat taagtaaagg ttttttaagt gctgcttgtg ggaaggaatc ttgctctaga
     9241 ttttgcaatt tctttctttt ttctttcttt actctataac aatagcccaa attcattcaa
     9301 ttgatagtag tgccagattg ggctcaaatg acctagaaaa atgtaaacgg ttactataaa
     9361 ataaaaatag aaaggaaaac aaatggttga taataagact gttgtgataa tgttggtatt
     9421 tttagctcgg aaaaatcttt ccttgtatga gttaacagtg caaacaaagt tttcaataaa
     9481 agttatcata gagcaaatta actatctcaa tagtttttta gcaaaaaatc acctaccagc
     9541 tattgctcac tctgctggac gttatcagtt attaggagat gaaaaagagc atgataaaat
     9601 tgtctcctta ttagaagctg agcaatttta cctaactcag gaagaaaggg tatgtctgat
     9661 ttacctttat agcttttgcc gaagagagtt tgtctccaat gtgcattatc aagatttttt
     9721 gaaagtaagt aagaatacta ctttatcaga tatcaagatg ctacgttcta aattagcaaa
     9781 aagaggtatc tcactcactt atacaagagc aaagggatat agtctagtag gagatgagat
     9841 ggataagcat caagttgctt ttcagatgat aactcaacta ctagaatctc ccattggttt
     9901 ttggtctttg aattatatct tgtcttcttg gaaattcgct ctctcatatg agaagttaga
     9961 aaagacggta gaatatttct atgaatcttt tcaactttct ccaatacaag ataggcttga
    10021 gaaatcttta tattttatca ttttaatact gtgtcgttat caaaggtcag tagatcgtgt
    10081 tttgcagggt tctcctattg tttctgaaca attaaaagag ttaacgacta ttattgtgac
    10141 taatttaagt caggatattt ccttatctaa accattagat cagaaagaaa aagattatat
    10201 taccttgatc ttatctggct gttttgaagg tgagggaact aaagatgatg atttctttga
    10261 agcattggca aaagcaatcg ttgatgagat ggaaactgtt tcacttctta atttttccaa
    10321 taaagaagag ttattacaag gtctgaagcg tcatattatt ccagcttatt tccgattgaa
    10381 atatggttta actggcgata gtggttatac acaaaatatt aaagagcatt acagtgattt
    10441 atttctattg gttaaaaagg cactacgtcc tttggaagag caagtaggat taatacccga
    10501 tagtgaaatt tcttacttcg tgattcactt tggaggctat cttagacaat caggtgatac
    10561 acaatcaatg tcttataaag ctttgatact ctgtccaaac ggcgttagtt catccttagt
    10621 gattaaggaa aaattgcgtg gattatttcc tcaaattcat tttcatcgtg tctctaaaat
    10681 tgagcagctg aaactgattg acaatcaaac ttacgatatg gtcttttcta ctatatttgt
    10741 agaaacaaaa aaacctaatt atctagtttc gctgatgatg acagcagagc aagtacaaca
    10801 gctcaaagaa ctggttattt cagattttcc caaagcttgc cttgacgatt tccaacttga
    10861 tcaattaata gcaactataa aaaaatacgc tcatgttcat tgtgaagagg aattaaaatt
    10921 ggctttgagg acaatggtaa aacaagatat atttagaaag gatgtgaggc ccttgttgca
    10981 ccaacttata acagaggaaa cttatcagac aagttctgaa caaatgaatt ggaaagaggc
    11041 aattcgttta gcggcaaaac ccttgttagc ttctggaaaa attacggaaa gttacccaga
    11101 agctatgatt gaaaaggtcg aagagtttgg tccttttatc aatcttggta aaggtattgc
    11161 tattccacat gccagaccag aagatggggt aaactctgtc ggtatgtcaa tgcttgtttt
    11221 ggaacagcct atttatttat tggatgaccc caaacaggaa atttatctct tattatgcat
    11281 agcagctatt gataatgaaa cgcatttgaa agctctatcg catttgacaa caatacttcg
    11341 tgataataat aatgttaaag ctttgttagc ttcacgtggg tatcaagata ttgaaatgat
    11401 tataaaacag gaggattaaa gatgttaaga attggaacag catgtggatc aggtctaggt
    11461 tcaagtttta tggtacaaat gaatattgag tctattttga aggatttggg ggtttctgat
    11521 gttgaagttg aacattatga tttaggtgga gcagatccaa gcgcagcaga tgtttggatt
    11581 gttggtcgtg atttggagga ttcagctggt cacctggggg atgttcgtat tttaaacagt
    11641 atcattgata tggatgaatt gagagaatta gtgacaggta tttgccaaga aaaaggctta
    11701 atctaggagg ttgatgccat gaaaggtcta ttggattttt tagttaatat tgccagcacg
    11761 ccagctattt tagtcgcctt gatagccatt atcggtttag tactgcagaa aaaaggtgtt
    11821 cctgatattg taaaaggtgg aataaaaaca tttgttggct tcttagtggt ttctggaggt
    11881 gcagggatag tccaaaattc cttgaatcca tttggaaaaa tgtttgagca tgcttttcat
    11941 ttggtggggg tagttcctaa taatgaagcc attgtagcag tagctcttac gaagtatggc
    12001 tcagcaactg ctttgattat gttagcggga atgattttta atattttaat tgctcgtttt
    12061 acaaaattta aatatatttt tctgacagga catcatacat tgtatatggc ttgtatgatt
    12121 gctgttattt ttgccgtagc aggctttact tcgttctcgc tcatcctttt tggaggtcta
    12181 gcgttaggga ttattatgag cgtatctcct gcatttgtgc aaaaatacat gattcaactg
    12241 acaggtaatg ataaggtggc acttgggcac tttggttctt taggctattg gttgagtgga
    12301 tttattggtg gaattgttgg agataaatca aaatcaacag aagatattaa atttccaaag
    12361 agtttatcat ttttacgtga cagtaccgta agtattacta tctcaatggc aattatttac
    12421 cttatcgttg cagtgtttgc tggtgaggct tatattgcaa aagaaataag taacggtgtc
    12481 aatggtcttg tttatgccct ccaactagct gggcaatttg cagcaggtgt attcgttatt
    12541 ttagcaggtg tacgccttat cctaggtgaa attgtaccag cctttaaggg aatctcagaa
    12601 aaattggttc caaattcaaa accagccttg gattgtccaa ttgtttaccc ttatgcacct
    12661 aatgcagtat tgattggttt tatctcaagt tttgttggtg gattggttag tatgatagtt
    12721 atgattgtga ctggcactac tgtcatttta ccaggggtcg ttcctcattt cttctgtggg
    12781 gcaacggcag gtgttatcgg taatgcttct ggtggtgtga gaggagcgac tattggtgca
    12841 tttgttcaag gtatcctcat cagtttcttg ccgattttct tgatgcctgt actaggtgga
    12901 cttggtttca aaggttcaac attctcagat gctgattttg gtttgaccgg tatcattctt
    12961 ggcgctctta atcatgtcgg cggtgcgata gctattgtta ttggtattgt agttatctta
    13021 attggacttt ttggcatttc atttgttgga aaatcaaccc ataaggaagg ttaataatga
    13081 catcaccaga agaaagatat tcatctctaa gacgatttgc aacggagatt cgtttaaata
    13141 cactagaaac gctaaaccat ttaggattcg gtcactatgg gggtagttta tcaattgttg
    13201 agactttggc tattctgtac ggtgatatta tggatataaa ccctgaaaaa ttcaaagaaa
    13261 gtgatcggaa ttatatggtc ttatcaaaag gacatgctgg tccagccttg tatagtacac
    13321 tctatttaaa aggttttttt gataaaacat tccttcattc gctcaataca aacggtacca
    13381 aactaccttc gcatcctgac cgcaatttaa ctcctggtat agatgtaacg acaggctcat
    13441 taggtcaagg cattagtatt gcaacgggaa ttgcttatgc tcaaaagatt gagaattcaa
    13501 gctattatac ttatactatt gtaggtgatg gtgaattaaa tgaaggacaa tgttgggagg
    13561 ctatacaatt tgctgcgcat catcaactgc accatttaat tgtttttgta gatgataata
    13621 aaaaacaatt agatggtttg acagctgata tttgtaatcc tggagacttt gttgctaaat
    13681 ttgaagcttt tggatttgat gcagtacgtg taaaaggaga tgacattgag gcaattgaca
    13741 aagctattaa aacttttcaa gattcaaata gtgtcaaacc aaaatgcatt gttttagata
    13801 gtatcaaggg acaaggtgtg aaagagttgg aggagttagc ttctaatcat catttacgac
    13861 cagatttaca acaaaaaaca atgttagagc gagcggtgat aagcttgaga gaaagtttgg
    13921 aggtggtaga atgagatgtt caaccaaaga aatgaggctt gtctatcgtg atttcctcct
    13981 tcaagctaat caagaaaata aacaaattac cgttttagaa gccgatttgt cgagttcaat
    14041 gtcaacaaat gtactggcat cagaatttgg aaaacgttat attaatctag gtattatgga
    14101 ggctgagatg gtaggtcttg cagcagggct cgccattaaa ggttacaagc cataccttca
    14161 tacttttggt cctttcgcgt caaggcgtgt ctttgatcaa gtatttttat cgttaggtta
    14221 ctcacaatta tcagctacga ttattggctc agatgcagga gttagcgcag aaatgaatgg
    14281 cggaactcat atgccttttg aagagttagg tctactaagg ttaattccaa aggcgactat
    14341 ttttgaggtt agtgatgata ttcagtttga agcaatttta aaacaaacat taagtattga
    14401 tggtctaaaa tacattagaa ctattcgtaa ggcctccact gcagtttatg agggatgcga
    14461 agatttttca aaaggtttta tacaattacg ccagggaaaa gatattgctc ttgtagcaag
    14521 tggtattatg gtttctagag cgatagaagc agccgactac ctcaaagaac tagggattga
    14581 ggcctcagtt atcgaccttt ttaaaattaa gccccttcca gaagaactga aaccattact
    14641 gatagatcaa tccattgtaa ctattgagaa tcataaccgt attggcggta tcggaagtgc
    14701 attatgcgaa tggcttagta tggagaaaaa cactactgtt agtcgtatgg gtattgatga
    14761 gcgatttggt caggtaggtc agatgaaata tttattagag gagtatggac tcgctgttaa
    14821 agatatagta gagcattgca aatcaattta taaatcataa caaaaaggca acattggagt
    14881 tgcctttttg ttattttctg aaatttttaa gttttgtata agtatcttgt ggtattttat
    14941 ccttactaaa aataaagggg tggtcgttat gcgagcttgg aaaggtattg ttttaatttt
    15001 atctagcata gtggtgacac tagtagcatg gcaaaatgca ggattaagtg aatttgttgt
    15061 accaggatta gctttgacaa gtttatcgct aacattcctc ttatcaacca aatttaggat
    15121 acttgaaagt tactttcaag gtattgaaaa tatgtatttt taccacaagg taatggctgt
    15181 attttcaatg attttgcttc tattgcacaa aattggtctt ggacaaggcg gacatggaag
    15241 tgagtttgca aagactattg gcagtgcggg attatatctt tttctaagta ttgtttttgt
    15301 agcttatttc ggaaattttt taaagtatga aatttggcgt tttattcatc gctttgttta
    15361 tttagtctat attttgggat tggtacatac ttttctgatt ttaggagata ggatactagg
    15421 gaatacttta ctcagtttaa tcgtattagg ttatgctgtc attggggtta tatctggatt
    15481 ttatattatt ttcctatatt ctagaatgcg ttttcgtcgt gtagggtacg ttcaaaaggt
    15541 tactcatctt aaccatgata caactgagat tgaaatagca atgaagcgtc cttatcgata
    15601 tgactatgga caatttactt ttttaaaaat ttatcaagcc ggctttgaat cagcagctca
    15661 tcctttctcg atatcgggtg gtcatgatag agtgatattt ttaactgtta aagcttcagg
    15721 agattatacg aaatccattt acaaacagct aaaagttggg acaaaaatag ccttagatag
    15781 agcttatggt catatgttat ttgataaaga caagaaagaa caggtttgga ttgcaggagg
    15841 tatcggcatc actcccttca tttcatttat tagagaaaat agtattttga ctaagagagt
    15901 tgacttcttt tatacatttt caaatcaaga caatttgatt tatcaagata tgttagaatc
    15961 ctatgctaaa gccaatccta attttaaact tcacttaaat aatagtagcc tccaaggaag
    16021 acttgatttt agtcagtctg tttttgaagg acaaccaacg atatttatgt gtgggccaac
    16081 tagtatgaca tctacttatg ctaaggtctt tcggcaaaaa gatgcaaagt ctcgtttagt
    16141 ctatgaagga ttttccttta gagacagttg gttatccata tttctattaa aaacttttga
    16201 caaagtttat tctaatttga taaaataaga gggtctataa gacaaaccaa ctttttcttg
    16261 gttttaggag tgccaatcct aagactcgga tgaagcaata aaaggagaat atattatggc
    16321 aatctcaaaa gagaaaaaaa atgaaatcat tgctcaatat gcacgtcacg aaggtgatac
    16381 aggatcagtt gaagttcaag tagcagttct tacttgggaa atcaaccacc ttaatgacca
    16441 catcaaacaa cacaaaaaag accacgctac ttaccgtgga ttgatgaaaa aaattggtca
    16501 ccgtcgtaac ttattagcat acctacgtcg tacagatgtt aaccgttacc gtgaattgat
    16561 tcaatcactt ggacttcgtc gttaatagac aacagagtca agtaaaaacg ctagaaaatt
    16621 ttctagtgtt ttttgtttta aacgtactat gaacgtaagt actttgaggg ggccaacagt
    16681 aagaaagtgt tttaacctat gaagagggtt attaagcaag ctaagattaa ccctagaaat
    16741 ttattttaat cattgtaaca aggtctgatt gctagtatta ataatcagac tttttacgtt
    16801 tacattaatg aatgataata cagcttcccc cacggatttt cgctactcaa ttttatagaa
    16861 ttatgttaga ataaagctta tacgtggttt tatgcaaagt tgagtttaga acagctagat
    16921 tgaactttgt aggaaacgcg taaacaaaaa aatatggaga ataatatgtc aaaacaagtc
    16981 tttgaaatga tttttgctgg taaaaaatta gttgttgaaa ctggccaagt ggctaaacaa
    17041 gcaaatggat cagttgtcgt tcgttatgga gattctactg tacttacagc agcagttatg
    17101 tctaagaaaa tgtcaacagg tgattttttc ccacttcaag tcaattacga agaaaaaatg
    17161 tatgctgctg ggaaatttcc gggtggtttt aacaaacgtg aagggcgtcc aagtacggat
    17221 gctaccttaa cagcacgtct tattgataga ccaatccgtc cgatgtttgc tgagggattt
    17281 cgtaacgaag tccaggtcat caatactgtt ctttcatttg atgaaaatgc tagcgcacca
    17341 atggctgcta tgtttggttc atcattagca ttatcgattt ccgatattcc tttcaatggg
    17401 ccaattgctg gagttcaagt tgcttatgtt gatggcaatt ttattattaa tccgacagca
    17461 caagaacaag aggcttctgc gttagaatta acggttgcag gtactaaaga agcgattaac
    17521 atggttgaat ctggagcgaa agaactttca gaagaaatca tgctagaggc tcttcttaaa
    17581 ggacatgagg ctgtctgtga attaatcgcc tttcaagaag agattgtaac tgctattggt
    17641 aaagaaaaag ccgaagtaga acttcttcaa gttgatccag aacttcaagc agaaattatt
    17701 gctactcata acatagcttt acaggctgca gtacaagttg aagagaaaaa ggctagagaa
    17761 gcagctacag aagcagttaa agaagtagtt ataggtgaat atgaggcgcg ttacgctgaa
    17821 catgaagaat atgatcgcat tatgcgtgat gttgctgaaa ttttagaaca aatggaacat
    17881 gcagaagttc gtcgcctcat tacagaagat aaggttcgtc ctgatggacg tcgtgtggat
    17941 gagattcgtc cattagatgc agtgattgat tttttaccac aagttcatgg atcaggtctc
    18001 tttacacgag gacaaaccca agcattatca gtattaacgt tagcacctat gggtgaagca
    18061 caaatcattg atgggctaac accagagtat aagaaacgct ttatgcacca ctataacttc
    18121 ccacaatatt cagttggtga gacgggacgt tatggagctg cagggcgtcg tgaaattggt
    18181 cacggtgctt taggagagcg tgcattagaa caagtccttc caagcttgga ggagtttccg
    18241 tatgctatcc gactagttgc agaagtttta gaatcaaacg gttcatcatc tcaggcttct
    18301 atatgtgctg gaacgcttgc tcttatggca ggtggtgtgc ctataaaagc tccggttgct
    18361 ggtattgcca tgggactcat atcagatgga acaaactaca cagtccttac tgatattcaa
    18421 ggattagaag accattttgg cgatatggac tttaaggttg cgggtacacg tgaagggatt
    18481 acagcacttc aaatggatat taaaattgaa ggtattaccc ctcaaatttt ggaagaagct
    18541 cttgcgcaag ctaaaaaagc acgctttgaa attcttgatg tccttcatgg agctatcgca
    18601 gagccacgtc cacaattagc accaacggca cctaagattg atatgataaa aatcgatgtt
    18661 gataagatta aagttgtcat cggtaaaggc ggcgaaacta tcgataaaat catcgctgaa
    18721 acaggtgtta agattgatat tgatgaagaa ggaaatgtat ctatcttctc tagtgatcag
    18781 gctgctattg atcgtacgaa agacattatt gcaagtttag tacgtgaagc aaaagtggga
    18841 gaagtttacc atgctaaagt agttcgtatc gagaaattcg gtgcctttgt caacctcttt
    18901 gataaaacag atgcattggt acatatttca gaaattgcat ggacacgtac agcaaatgta
    18961 gctgatgttc ttgaaattgg tgaagaagtt gatgtgaaag tcatcaaaat tgatgataaa
    19021 gggcgagttg atgcctctat gaaagcatta ttaccacgtc ctccaaaagc tgacaatcct
    19081 aaaaaggaat cttaatatga caagtacaaa cgagttagac attagattgc gtgcttttat
    19141 caatgcaccg gataattttt tagatagtat aggtcttgtg aatgccttgc accactctac
    19201 ggtttgggca agcaaggaac cctatgctat tcaagtagat ggtcaagaag ttgttcctgt
    19261 ttttacggat attactgacc ttaatcattt caaagaggag caagaaagtg ctcgagatat
    19321 gttttgggag agtcgtcgta gtttagacgt tcttgatgaa gctatttcac atggcttagc
    19381 aggattggtc tataatttaa aaaaagaagg cgattttggg aactcgacta ttttctactg
    19441 tgaagatatg gtgcaattta tgaacaatta caccacaatc ttaaaccagt tgttaaatga
    19501 agataacatt gttgccgata tcatggataa aacctacttg gtacctgcct ttgtccatcc
    19561 acgtgaagaa ggtagttttg accgtttttt ccctacaatg tctacccccg aaggaaaaag
    19621 ttatgttcca gtattctcaa accttttaag ttttgaaaaa tggtataacc ataatgattt
    19681 tggtggtgct tttaggaaag cacaaggagt aattttagcc tggacgattg acgatattta
    19741 taaaccgcgt aatggagaaa atgagattga tgataccttt ggagtagcca tcaatccatt
    19801 tgatgaacaa caagttttag ttgattggtc tgatgtagaa aacgattgag gaaaactatg
    19861 gggtggtgga aagaaagtat agctattgtc aaagaacaag atccagctgc tcgtagttct
    19921 ctagaagtta ttttaactta tccaggaata aaagctcttg cagctcatag gctatctcat
    19981 tttttatgga atcataattt taaattgtta gcacgtatgc acagtcaatt ttggcgtttc
    20041 tggacacaaa ttgaaattca tcccggagcg acgatttcgg aaggggtttt tattgaccac
    20101 ggttcaggat tggtcattgg agaaactgcc attgtagaga aaggtgctat gctataccat
    20161 ggggttacat tgggtggcac aggtaaagac aaaggcaaac gacacccaac catacgtaaa
    20221 ggtgcgctaa tttcagcaca ttctcaaatc atcggtccaa ttgaggttgg agaaaatgct
    20281 aaagtcggtg ctgcagcagt tgtccttgca gatgtgccag cagacgtaac agtagttggt
    20341 gttccagcta aagttgtccg tgtccatggt caaaaggatg atcttcaaat ccgaagtatt
    20401 gaacatgatc gagaagaaag ttattattcg tccaaacttt aggaggtatt tgtgaaggat
    20461 tatgtaacaa ctcaaatatt atcaatttta ggtatactag caggagcttg tttaagctat
    20521 ttaaagtttg cacaaggtaa ctctctctta ggaattattt gggctgcctt tgctttaatg
    20581 tatattgttc gtttgtataa agcttatcaa aaacagaaaa aggaaaaacc atgataaaaa
    20641 tttacgacac tatgactcgc agtttacaag attttatacc tctcaatgaa ggtaaagtca
    20701 acatgtacgt ctgtgggcca acagtttata actatataca tatcggaaat gcccgcagtg
    20761 tagtagcttt tgatactatt cgtcgttatt ttgagtattg tggttaccaa gtcaattata
    20821 tttctaactt tactgatgtt gatgataaaa ttataaaagg tgcagctgaa gcaggtatgg
    20881 atacaaaatc cttttcagat aagtttattt cagcgtttat ggaagatgtt gctgcattag
    20941 gtgtcaaacc tgctacaaaa aatcctcgag ttattgacta tatggatgag attattgatt
    21001 ttgttaaagt tttagttgat aaggaatttg cctatgaagc taatggagat gtttatttta
    21061 gagtaagtaa atcacatcat tatgcaaaat tagccaataa aactttggaa gatttagaga
    21121 ttggtgcaag tggtcgtgtt gatggggaag gagagattaa agagaatcct ttagattttg
    21181 cactttggaa atcagctaaa tctggggaag tctcatggga aagtccttgg gggaaagggc
    21241 gcccagggtg gcatattgaa tgttctgtta tggcaactga gatccttggt gatactattg
    21301 atatccatgg tggaggagct gatttagaat ttccacatca cacgaatgag attgcacaat
    21361 ctgaagctaa aacaggtaag acctttgcaa attactggat gcataatggt tttgttaacg
    21421 ttgataacga gaaaatgtct aaatctcttg gtaactttat tactgttcac gatatgttga
    21481 agagcgttga cggacaagta atacgttttt tcttagcgac acaacagtat cgtaaacccg
    21541 tgaatttcac tgaaaaggcc gttcatgatg cggaagttaa ccttaaatat ttaaaaaata
    21601 ctttcaattt accaatccaa gagaatgcta atgatgaaga attagagcaa tttgtgaaag
    21661 catttcaaga agcgatggat gatgatttta atacagcaaa cggtattact gttatctttg
    21721 aaatggcaaa atggattaac tcaggacact atacttcaaa agttaaggaa acccttgctg
    21781 aattattaga aatttttggt attgtattcc aagaggaagt gcttgatgct gatattgaat
    21841 ctttaataga acaacgccaa gaagcacgag ctaatcgtga ttttgcaact gctgatcgta
    21901 ttagagatga attagcaaaa caaggaatta aattgttaga tactaaggat ggcgtgaggt
    21961 ggactcgtga ttgatgttcg tttgattaat ggtattgctt tagcttttga aggagatgca
    22021 gtttattctt tgtatatccg acgccattta attatgcagg gttttaccaa acctaatcag
    22081 ttacatcgga aagcaacgca atatgtttct gctaatgccc aagcattgtt aattaatgcc
    22141 atgttagaag aaaatatttt aactgatgaa gagcagttaa tttataaacg aggacgtaat
    22201 gcgaatagtc atacaaaagc taaaaatgct gatattatta cctatcgtat gtctacaggt
    22261 tttgaggctc ttatggggta cttggatatg actggtcaga taaagcgctt ggaaactcta
    22321 atacagtggt gtattgaaac cattgaaaaa taaacaaaaa gagaggctat atcctctctt
    22381 ttttatagag acattaggat ttgttggtaa aatattgcta taaaaagctt tatgctatag
    22441 cattcatgat ataatatgac tatgaaagat aaacaattta aagaagaatc aagtgacctt
    22501 gtctatggct tacatgctgt gacagagagt ttaagagcta atactggtaa taaattatac
    22561 ttgcaagatg acttaagagg aaagaacgtt gataaagtca aagcactagc gactgaaaaa
    22621 aaagtatcca tttcctggac tcctaaaaaa atcttatcag acatgacaaa tggtggagtt
    22681 caccaaggat tcgttctaaa agtttcggaa tttgtctatg cggatttatc agagataatg
    22741 acaaaggcag agaatgaaga aaatcctctc attttaatcc ttgatggtct gaccgaccct
    22801 cacaacctag gttctattct acgcacagct gatgcaacca atgtaacagg tattatcatc
    22861 cccaaacatc gttctgttgg agtgacccca gtggtttcaa agacatctac aggagctgtt
    22921 gaacatgttc ctattgcaag ggtaactaat ctcagtcaaa ctttggatac tttaaaagat
    22981 aaggacttct ggatatttgg cactgatatg aatgggacac catcgcataa gtggaatacc
    23041 aaaggtaaat tagccctagt tattggtaat gaaggaaaag gtatttctca taatatcaaa
    23101 aagcaagtag atgaaatgat tacaattccc atgaatggtc atgttcagag tttaaatgcc
    23161 agtgttgcag ccgctatttt gatgtatgaa gtatttagaa accgcctatg aaaaaacatt
    23221 ctatcttact cgttgatggc tacaatatga ttgctttttg gaaagatact cgacagcttt
    23281 ttaaaagcaa tcgtttagaa gaggcaaggg aagtattact tagaaagcta aatcattacg
    23341 cacatttcga acacattgat attatttgtg tatttgatgc ccagtatgtt cctggtgtac
    23401 gtcagcgtta tgaccaatac aaaatatccg ttatctttac tgaggaggac gaaactgcgg
    23461 atagctatat tgaacgagca gcggcagaac ttaatcaatc tgtgttaaat ttagtctccg
    23521 ttgctaccag tgatttaaat gaacaatgga ctatattttc tcagggtgct ttacgtgtct
    23581 cagctagaga attggaacag cgtgtagcga cagtaaaatc agatttggat aagatgtcca
    23641 gtcaaatcga tttgagtacg ccaaagttaa ggccgtggaa tgatgaacaa ctaggaaaac
    23701 tcaaggattt tttagatggt atgtaaaacc taacacttta ttagccagcc aacaattttt
    23761 ttgatataat attggaacta ctttgattat caaataaaaa taatagattg aggtaacaat
    23821 gacttttaaa atactaacgg attcgacatc ggatttagat gaaaagtggg ctcaagagca
    23881 caatgtcgat attataggtt tgaccattga acttgatgga aagacttatg agacagtcgg
    23941 agacgaaaaa attactagtg acttcttgtt ggagcgtatg caagagggag caaagcctac
    24001 aacaagccaa attaacgttg gtcagtttga agaagttttt tcaacctatg cagaaaatga
    24061 ccatgccttg ttatatttag cgctatcttc acatttatca gggacttacc agagtgcaac
    24121 tattgcgcgt gagatggtct tagacaaata tcctgatgct caaattgaga ttgtggatac
    24181 aatggcagct tcatgtggtg agggtgtctt agcaatgtta gcaactaaag agcgacaaga
    24241 aggtaaatct ttagaagaag tgaaacaaaa aattgaatca cttctcccta aattaaacac
    24301 atacttttta gttgacgatc tcaaccattt aatgcgtagt ggtcgcttat caaaaggtgc
    24361 agctattatt ggaagtgttg ctaagattaa accattgctt aagttagata gcgagggtaa
    24421 attagtacca tttgccaaga caaggggccg taaaaaaggt attaaagaga ttgtgactca
    24481 ggcaacaaaa acactatctt atagcacttt aattattgct tatagtggag agaaagattc
    24541 agcccaagtt atgaaagaac agttattagc agatgagcgt attgaagaag ttattattcg
    24601 tccattaggg ccagtcattt cagctcatgt gggctcaggt gctttagctc tgttctcttt
    24661 aggggaagaa aaccgataac caatcaaagg gcataacagt acgttatgtt cttttagtat
    24721 atatttcata aaatgtattt tatatgatgc tatgaaacta taaaatagta ggagacctat
    24781 tatgaaaaat aaaataagaa ctatcctcaa tatgatgaac gacaacaaca attggtttta
    24841 caaagttatc aaaaatgttt ttagacgatg gtttttatct tatggcttga aagtgttttt
    24901 ggaaaactaa taaaggggag cgtatcatct gattttataa taatattaac tttatttagt
    24961 ggtgtttcta ttttgttacg acttctaatc taaaaagagt ctctccgttt gttgatgaaa
    25021 gattttggaa aatctttttg ttgctggcgc atttgccctc ataggaataa ctattttatt
    25081 gatgctttta ctaggaaggt tgatattgct acttattttt caaggggaga tgatgcaatt
    25141 ttagcagtta taggaatatc attgatagct atgggaactt cagtgtacta ccataagtat
    25201 aaggacagat agtaatatga aaaatttacg cttaaagaca gctagagcta gtatggattt
    25261 attacaacag tctctagctg aaaaggtcgg tgtatcatgt cagacaatag ccgccattga
    25321 aaaaggaggc tataattaag cgattaaatt gtgtcaagct ttgaataaaa ctgtagatga
    25381 tttattttgc taaattctca aagtaagttc cacctctagt agaaatggaa tttagtctga
    25441 taaaaaccaa taaaaaccaa taaaaaccaa cgaaaatccg tctcagacta agttccgttg
    25501 gtttttataa tacttgattt cataggtttt cagagttaaa aatcgcaaaa aa
//
LOCUS       DBJYSH010000012        24989 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.012, whole genome shotgun sequence.
ACCESSION   DBJYSH010000012 DBJYSH010000000
VERSION     DBJYSH010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 24989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24989
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.012"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            567..1001
                     /locus_tag="ACUBV3_000409"
     CDS             567..1001
                     /locus_tag="ACUBV3_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HHU3846944.1"
                     /translation="MTFFPEINIQKTKSNAKRKLREYPRWRRIANDVDTQKVTATYSF
                     EPRQSHGTPSKPVERLALNRVSAEQELEAIEQSVSMILEPEKRRILYDKYLSPYKNAD
                     KVIYTELCMSESFYYDTLDAALLAFAELYREGSLIVEQGVFD"
     gene            1167..1245
                     /locus_tag="ACUBV3_000410"
     tRNA            1167..1245
                     /locus_tag="ACUBV3_000410"
                     /product="tRNA-OTHER"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
     gene            1762..2571
                     /locus_tag="ACUBV3_000411"
     CDS             1762..2571
                     /locus_tag="ACUBV3_000411"
                     /inference="COORDINATES: protein motif:HMM:NF014177.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HHU3846945.1"
                     /translation="MKQNFGTDKKIYDIFGGGGAITAECMLNNLEVHYNDLNNDVTDM
                     FKRVITQDREWLKTLIISREEFLKIKTKTNKTVDDNIKLLVNSFGNNSISFLYNIELS
                     DIKYKLAVEIINKHDVFSGYKQTDTYKETCLLCEEEKLGKIGNLQRLQQLEQLQRLQQ
                     LERLQQLEQLERLEATNLDYKAFSNVDGAIFYLDPPYEMTSQHSYIGDTFDSQLFYDW
                     AYEMSKENIVIVSSYEISDDRFKCVYEFKTARSTMQGGSACGKTEKLFMVV"
     gene            complement(2624..3007)
                     /locus_tag="ACUBV3_000412"
     CDS             complement(2624..3007)
                     /locus_tag="ACUBV3_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HHU3846946.1"
                     /translation="MLIYPAVFTHDNETNSIGVRFPDVPEAITFGNDLKHAYEMAVEV
                     LGFALEDYTDYPKASSVSDLKEQYPDSDIALIGIDMIAYMKKYHSKKVRKNVTIPEWL
                     NNAAEDKNLNFSQVLTEALELKLQA"
     gene            complement(3051..3239)
                     /locus_tag="ACUBV3_000413"
     CDS             complement(3051..3239)
                     /locus_tag="ACUBV3_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008089605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HHU3846947.1"
                     /translation="MPRTAREIIKLLKKNGFVKVSQNGSHAKYKNFETGNITIVPMHK
                     GDMPKGTEDDILKKAGLK"
     gene            3396..3845
                     /locus_tag="ACUBV3_000414"
     CDS             3396..3845
                     /locus_tag="ACUBV3_000414"
                     /inference="COORDINATES: protein motif:HMM:NF015550.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HHU3846948.1"
                     /translation="MCGNATDAAIKAGYSKKTAGQIGEQNLKKLEIKQAIQSRMKVLE
                     KSSIATADEVLRVFTQILRQELTEEVTELNQITGEFVTIEKQPSIAEVIKAGSELMKR
                     YPTNLELKKINLEIEKLKSQIGGDEGQDEKIANFLNMVKGAISDGVE"
     gene            3832..5073
                     /locus_tag="ACUBV3_000415"
     CDS             3832..5073
                     /locus_tag="ACUBV3_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HHU3846949.1"
                     /translation="MELSKLYTRKQLQVLDYIWNNDWFICGLHGAKRAGKTVVNNDTF
                     VTELYRVRKIADDLKIDEPIYILAGTSSTSIQNNVLQELYNKYGFEPKYDKHGSFTFC
                     GVKVVQVYTGSISGLKRARGFTSFGAYVNEASLANETVFKEIISRCSGEGARIVWDSN
                     PDNPNHWLKTDYIDSDDDMIIDFSFKLDDNTFLSKRYIESIKSATPSGKFYDRDILGK
                     WTVAEGAIYSDYDKNVHEVEELPKMTRFFAGVDWGYDHYGSIVIIGEDNNGNYYLVDG
                     IAEQYKEIDWWVTRAKEFIQIYGNVTFWADSARPEHVARFKRERIKARNGRKEVVAGI
                     EYIAKSFKDKRLFIKRGCIPRFFDEIYQYKWKPNSTKDEPQKEYDDVLDAIRYALYSQ
                     HKEDSISKTNNFNVLYQGLKN"
     gene            5086..6579
                     /locus_tag="ACUBV3_000416"
     CDS             5086..6579
                     /locus_tag="ACUBV3_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000930109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0046798 - viral portal complex [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0051027 - DNA transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HHU3846950.1"
                     /translation="MAHIEDFIDSTGEHKLLELRFHRESRMRYQISDISALFDDNYKI
                     LLEYLKHHENIQKPRIQELLDYAEGNNHEVSKSGRRQDEDMADVRAIHNYGKYISTFK
                     QGYLVGNPIRVEYDDEVNNELLKELAKKNNFHQLNRQLIKDLSKVGRAYELAYRSADD
                     KTKVIRLDPRETFIIYKNDVDKDSLIGVRYYNKSQIDKTDKTVEVYTSSEVIFFEFNG
                     ELTETDRQPHAFGAVPITEYLNTDDGLGDYETELSLIDLYDSAQSDTANYMQDLSDAI
                     LAIIGRVSFPEYVDTQEKAITYLRAMRKARLLNLEPPVDSEGREGSVDAKYLYKKYDV
                     QGTEAYKNRIAEDIHRFTNTPDMTDNKFAGNQSGEALKWKVFGLDQERVDLQALFEQS
                     LKRRYKLIAHVSEFLSEIKDFDISKLKIIFTPNLPKSDQEKINDFKALGGELSNKTKM
                     FVTGIVDDPDEEEDKIMQESQSGSLLAQKLEAQTRMSDKELAHGHAH"
     gene            6563..7534
                     /locus_tag="ACUBV3_000417"
     CDS             6563..7534
                     /locus_tag="ACUBV3_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HHU3846951.1"
                     /translation="MDMHTKEGKSYWRKRVKKEMEAKDKKDVSLGKSMKQIHDYHFRE
                     IEKEIESFYQRYADKEAIDLKLAKKAVSDVDINAYQKKAKELVARANEMRKEGIKVTK
                     ANFTHQENIDMAVYNLKMKVNALELLQLNIDLAMQNLSEDEYKATKRFLEDGFEEELK
                     FQSGLLGTSVSSQNDIKNLAKATINANFKGATWSENIWQRQDDLRKIVKEEVYKAVTK
                     GDNAIKLSNKLKKEFEVSDSYARRLAITEHARVQMEVSKISIEDNGFNGFEILPEPSA
                     CSICKGIASDGPYPMEKWDTGNTAPPFHPHCRCAVVGEDIEHKKGKK"
     gene            7531..7734
                     /locus_tag="ACUBV3_000418"
     CDS             7531..7734
                     /locus_tag="ACUBV3_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3846952.1"
                     /translation="MNKRIKKKRKLEYYIASLVAENVMFSKELIKQHERIEQLEKIVE
                     HNAQATNNELSRIKKHSKKKWKK"
     gene            7845..8423
                     /locus_tag="ACUBV3_000419"
     CDS             7845..8423
                     /locus_tag="ACUBV3_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HHU3846953.1"
                     /translation="MADLVNNGVVDEVTQEEVETQEEVKAEATSEKTFTQAEVTEMIQ
                     QNVNRAVAKAHKDAQEQFKAEQDEAKKLAKMNSEEKANYETQKLLEELQQLKDDKTRN
                     ELTAVARKMFSEADINVDDDVLSLAVTLDAEQTKANVTKLASAFAKAIADDRKSLARQ
                     ATPSIGSGSITTQSNFGASLAERAGKVNTKLF"
     gene            8433..8816
                     /locus_tag="ACUBV3_000420"
     CDS             8433..8816
                     /locus_tag="ACUBV3_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HHU3846954.1"
                     /translation="MNKRTVKTSKEILHNLPYEAISVTLDANAFGKGLASAGTVLAGD
                     GASVFKDRTKKVKPVRNAEVSGADHIDGILLTDVDLSKGDATGALVYRGTVNSDKLVD
                     QELAKDTSTLATKLPHIVFVKGGTK"
     gene            8816..9868
                     /locus_tag="ACUBV3_000421"
     CDS             8816..9868
                     /locus_tag="ACUBV3_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HHU3846955.1"
                     /translation="MALIYDVVTSANIKGFYDKQQANVDLTLGEKAFPSKQQLGLKLS
                     FIKGAAGKPVSIKAAAFDTKVPLRDRIAVVLLDEEMPYFKEGMLVKEADRQQLNVLAQ
                     TKNQELIDTVLSTIFNDETTLIAGAKARLEAMRMEVLSSGKIHINSNGVMKDIDYGLT
                     GTQTTKSEQKWSEKDTANPLADIEKAIETVTERGHVPEAIVLNSKTFGYIKNAKATVK
                     AIKPLAPEGSIVTKAELKSYLSEELGLNILLKDGVFVNDAGESKKYFPDGVVTLVPNG
                     NLGYTVFGTTPEQSDLMGGQATDAQVSLVETGIAVTTTKTTDPVNVQTKVSMIALPSF
                     ERLDEVQIVTSSEVSL"
     gene            9881..10135
                     /locus_tag="ACUBV3_000422"
     CDS             9881..10135
                     /locus_tag="ACUBV3_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HeH/LEM domain-containing protein"
                     /protein_id="HHU3846956.1"
                     /translation="MALVIEAFRDKETGFIYKVGEEYNGARVEFLTGKGVLKATDTQS
                     TNFSNLKVDELKRGLDELGVNYDSKAKKAELLELLESHTD"
     gene            10151..10504
                     /locus_tag="ACUBV3_000423"
     CDS             10151..10504
                     /locus_tag="ACUBV3_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HHU3846957.1"
                     /translation="MEKINTQTIINNVKLDLDINDTLQDKLLEMLLKRITDHFSAEYG
                     TNEIDSAFSFVLEDCLIARYNRRGAERAKSESVEGRAITYYDFLNEFEPYDLMIKSKL
                     NISNQKSKKGGLYFL"
     gene            10501..10800
                     /locus_tag="ACUBV3_000424"
     CDS             10501..10800
                     /locus_tag="ACUBV3_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001270064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3846958.1"
                     /translation="MRYNDRVTLLIKANGEPRYDPTLGKRVGGEVKKDVVPCNISEQG
                     IELSNLLDEKLDLDRRIVRLRHSVKKVDRVLIKDKAYRVVTSRNKALYVEEIVND"
     gene            10793..11173
                     /locus_tag="ACUBV3_000425"
     CDS             10793..11173
                     /locus_tag="ACUBV3_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017827930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HHU3846959.1"
                     /translation="MINLTLEGEHELLSALKKEVTFENKRKAVRKNGRKLHKKAVKNA
                     NFNGHYKKRKGEEYVFVKPTGATRRSIKLEFSNQSTVAKVKAGTNYSGYLETGTRLME
                     EQPFMKPALDSVIDNFIKDLARVE"
     gene            11170..11559
                     /locus_tag="ACUBV3_000426"
     CDS             11170..11559
                     /locus_tag="ACUBV3_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HHU3846960.1"
                     /translation="MMKQPDQQLFDEIFKRLTDIGLTVYDFLPPLGTKYPFVVMGDTH
                     IIPMATKTQLIGKCSTTINVWGDGTNRKVVSDMIAQIMLEVSKISQIDGNKWAMVMNE
                     SDTQILKDNSTNENLYHGILNIYFNFV"
     gene            11568..12101
                     /locus_tag="ACUBV3_000427"
     CDS             11568..12101
                     /locus_tag="ACUBV3_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail protein, TP901-1 family"
                     /protein_id="HHU3846961.1"
                     /translation="MVTSSPIYGKDNILMFRVLGDKAAAAKLSFQTEHKWKYSRKTDA
                     KITKDGAINSDKGLEVTLEIKGVASRDELNTTLKNAVLEGKQIEVWDIDLNSNNDSDG
                     KYDAEYAIGRLGSWEVPSNVEEFSEISTEMAIDGKPVKGKATLTKEQIKAIQYVFKDV
                     TKIDESSVSSPGSVHSR"
     gene            12174..12533
                     /locus_tag="ACUBV3_000428"
     CDS             12174..12533
                     /locus_tag="ACUBV3_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly chaperone"
                     /protein_id="HHU3846962.1"
                     /translation="MKEITISGKNYPLNFGFDFIREMDKRFFVDNNGFKFGTGVQTAV
                     MQLAAKNPLILEDIILSATHTEYSIPKKEDIEKWAIEQAENGKLGEVLDDFLTILKKA
                     PLLKTQVSPILKALEEG"
     gene            12635..12913
                     /locus_tag="ACUBV3_000429"
     CDS             12635..12913
                     /locus_tag="ACUBV3_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3846963.1"
                     /translation="MTVKEYNLRIKGYLLKRLESERDIYLSAFVNRNVKAANSKGEYV
                     YREFEEFYDYEKRKKQVLSASVDNTINPKIIDRANRVKELRERGGLNV"
     gene            12906..15554
                     /locus_tag="ACUBV3_000430"
     CDS             12906..15554
                     /locus_tag="ACUBV3_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HHU3846964.1"
                     /translation="MSSRAYTVQAILKATDTNFTSTMNKVQSAAQATIDKIKSIKDSN
                     ISTLGKIGEYTTMVGQGMQSVGRSLSKYVTLPIVGLGVAAAKTFGDFESQMNRVKAIS
                     GATGADFEKLRKQAIDLGASSVFSAKEVAQGQEMMASAGFNANQILAASPGVMSLAAA
                     SGGDLALASEAAATAVNMFSLNASEATHVADVFAKAAADTNSEVADMAEALKYAGPVA
                     GAMGISMEETAAAIGIMSNAGIKGSQAGTTLRTAITRLAKPTDQMQAVIDGLGLSFFD
                     TNGKMRSLTEITGQLREKMSGLTDQQKSAALSILFGKESLSGMLALINATPGELSKLT
                     EGLKNSKGAADKMANTMNSGLKGAIEQLKGSLETAGITIGGILNPLLQGVIGKIQAVI
                     DWFNKLSPAGQKLAVIIGGIGAALGPLLVIFGTVIIFIGQVTASLEGVSTAFTAIAGI
                     FSIISGPVILVVAAIGAFIAALVVAWNTSETFRNTIISGWKSMVDAVTPLIETLKQLI
                     LSIFTFIQANSATFIGGLKLAWSALVEALSGLFLVITGVVQVGMSVINTTIKVILAII
                     NGDWNAAWTAIKSGVGAIWEGIKNIIIGGLTFLASLFVSMLGVFVSVFSAGWSAIKGI
                     FSVTLKFLVNVVAAGMSAIGNEISSKINTAKSVAILAFNAMKNGISTAINAAKEVVSS
                     VVNRIKNILNSLANINLGAAGRAIMDGFLNGLTSAFEKVKNFVGGIAGWIQEHKGPIS
                     YDRTLLIPAGRAIMGGFNKSLTDSFEPVKKNVSSMASRISEEFQNGLNKLKDINISEI
                     TEGLQSSFDVTHYASFGGLNDSARKLEDDRFVALKDAVLAIRDFANRDVVVTVNGKEL
                     ARTAGDNFTEYQKQKEIMNNRMKGLI"
     gene            15555..17129
                     /locus_tag="ACUBV3_000431"
     CDS             15555..17129
                     /locus_tag="ACUBV3_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="distal tail protein Dit"
                     /protein_id="HHU3846965.1"
                     /translation="MANFSYKGVDLSPFLNCLNIVRTIGNNRSIATRKINELGEAIQS
                     VSFGAKTIFVTVSFKTKEIGASKFVDTTEPSTYSYENLNKLREKIAGILHSKTTFKLT
                     LPDEPDRYYMAVPKGDIDLKGISDWYDETVIEFYIPDGVAHSTTYKKFLDYTRDGNKL
                     TFKLQNEGNTNALPIIKIKHNSENGYIGIANETGAFALGSSEEEDGTIVHRNEVLFDY
                     SKAIAKALEGAPNVAKLNHMPPSFDTELKRMRIDNILGSGKGGEYVVIGNRGTTPGYT
                     EHVGTRTFIINPDSNGEYTLNEHLWWQQIFIATAQDQKGFLKLCVTGIDDEGNDEFLY
                     GIETYKRKNGFEAEYNFFALDDDGVGWRFYKQFEFQADRNYHNPFSMDRSRAVEIFRE
                     EDKFRIYFNGAHHHATVPSLKGKKSRKIHLAMGTCSDSSKYINYNLFEKVNFEKMGVS
                     HYNNIVNKYQPGDEVIINFENDTVKTKDIDSLQDMVLGSQPISIPPGESELVINVSKF
                     SSTDPDIELLLEERWL"
     gene            17120..20947
                     /locus_tag="ACUBV3_000432"
     CDS             17120..20947
                     /locus_tag="ACUBV3_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HHU3846966.1"
                     /translation="MVVITLVIHDAKLHPVLLLDNERQGALNYYDDLWTRQLTTGSSA
                     FEFSVYKKSLLGDNPLNHKYHALNDQAFVSFVHKDKVQLFNIMRVEETETTIHCYCEN
                     LNLELLNEYCNAYKATKAMSFEEYLVQFDILNWGALTIGTNEVKDKKLTLEWTGQDTK
                     LARILSIANNFDAEIEFETQLHNNHTFKAFIVNVYKEYEEGVSYGVGRDRSDIVLRYQ
                     KNVTGITKKLDKRQIYNAIRPYGKKTVKGERVISNPVTRKVTKTVGSNRTYLGGDLKY
                     YGHTIKKANVQSIINYAVQYNILPSGIICQLYLESLWGDSAVGKRDNNWAGISGGAQT
                     RPSGVKVTTGMARPPSEGGTYMHYASVDDFLKDYTYLLAKQGIYNVVGKKNIADYTKG
                     LFRAGGAKYDYAAAGYQSYTNLMTNIRNGINKVTGNILNTIDKLWQTPVQPITAVNVA
                     RRATKTMQALNEATRLKGRRIGSGQCYALSGWYAKKLDGAWIDSSVGGIRGRIGGGMA
                     AALIGTDYNWGAYGWKLDRSPNAGNLQAGGIYNVKANFGAPFYTTQWGHTGIIKSVSK
                     TRVTVLEQNYAGRMYVMENSYEINAFARGLQTVCYPREIAQGMAVNGATTQQVSGGTQ
                     ISYEEVVQEAQTESYEEEQIIYIDNSIYKEWKDENGKVEYYLKNGFLYAPLSRDRYPS
                     VLTGNETRDNWIRKDMEVETDSQDVLISTALKDLKAHAYPAVTYEVDGYVDLELGDVV
                     RIQDDGYEPPLILTARVIEQEISITNPSSNKTKFSNFVEKESQLASDLISDMLRLYDE
                     SIPYDIQLATSNGVAFKNGVGESVLTPNLQKNGKDYDAIYFYKNGDSLIEIGPSLTVK
                     ASDFNHVLNITVEAYVNEELVASTQISFTDTEDGEKGDDGATSWTAWANSKDGKVDFS
                     ITEAKNRRFIGTYTGLTQSTNYLDYKWIDMSANVVIGTQNLLDGTKSFSGSWFTEGTI
                     FETTKISEYPFEFKKWKSGNKVSHTIEFDVKAGVTYTFTAAIARENAGRLYFYLYDLF
                     ANHITSNTPRETIIENVTTDIQMFKVTFVPLRDGKIKPRFAMLASDAGWFMTGGYMLV
                     KGNKSGDWQESEADKASNLDSKADQELTRAQILALEERTAIARENAIAEAMQHTLSEV
                     ETKWKLWYDLNTIDEKQKVANDIAQLFDRTTEFKQLLGEASAKFSFINNETLIGEEGI
                     AIGDKDGKAKLFLSNDSISFVTNGVAQMTLTGDTLTIKNGLFTERIQIGNFVEEVYDR
                     NPLFNVIRAIRNS"
     gene            20958..22970
                     /locus_tag="ACUBV3_000433"
     CDS             20958..22970
                     /locus_tag="ACUBV3_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF859 family phage minor structural protein"
                     /protein_id="HHU3846967.1"
                     /translation="MGTSTFSGTWGNNLTLDVRTSYTQNLVGNYSTVTVKVYEKISSY
                     GYIDYPGERTMTIVVDGKSYNESVNVDINYGQTKELGTYSYRINHNSYGSKPKFNVTV
                     TLPINFSNYGSASVTNSVSLPTIKRASTATIASGNIGSAVAITIKRQSDTFRHTLKFD
                     FKGLTGTIASLVDTSYSWTLPASLSVLIPNDRSGTGTLIVETFTSDSQKIGENKYTFT
                     ATVPDIAAYKPILASISLSDANTLTGSLITGNNYVRNMSKLKVSFGNSAGANGSTISS
                     YNAEIVEKGKAIFGNGSVFDMLDFVGTATIRATVTDSRGLTSEPVDTKINVIDYFLPI
                     VTSAKVVRSQQNPDILQVLPFVKIAPIIVGGIQKNQLKMSVSVAPYNTGIYAVDSGAA
                     TNTWSTISQMSGAPLNLGGTYDKSKSWLVKISVSDNLMSATPIIQPVASEFVLVTKAP
                     SGVAFGKIWEHGIIDAKGDVYVDGNIYCGDKPIQQKQLALNNGGSFRHDDTDLNSLQD
                     TGFYCVFRGANRPTGAGPGYVTVVRHETSNYAYQQFYDRTNKTIFTRVLENGVWSGWS
                     EYVKKDSLPTTIDTGWQSIGNGFSYRQTGSTVTVKYNFATNGIDKLTVGSMPTNLITS
                     DMMFAVTAWTIQLNVLNVQVSADGRILWFNPSKWTVNVKGQIQWTI"
     gene            22984..23310
                     /locus_tag="ACUBV3_000434"
     CDS             22984..23310
                     /locus_tag="ACUBV3_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000404431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1366 domain-containing protein"
                     /protein_id="HHU3846968.1"
                     /translation="MEILNKYPVMLEDKSIAKVNAIVAVDLPHVRGNLTFDLPVDFDN
                     KSFAETLEKCEQIFYDEKYKDKAQSEKMTELSTSTSTGTQTLINLISTLYAKEVLKDE
                     DLIAIG"
     gene            23285..23497
                     /locus_tag="ACUBV3_000435"
     CDS             23285..23497
                     /locus_tag="ACUBV3_000435"
                     /inference="COORDINATES: protein motif:HMM:NF049101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD1375 family protein"
                     /protein_id="HHU3846969.1"
                     /translation="MKILLLLVRIFLQEEGIDMMIKLFAIDLYYGRMAWSSFVKKGFS
                     EFINNKTKEQLAIMCDEELLAEILAS"
     gene            23510..23812
                     /locus_tag="ACUBV3_000436"
     CDS             23510..23812
                     /locus_tag="ACUBV3_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000215499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3846970.1"
                     /translation="MTVEQAERIAQSQFVWAILFILLFMIVVGYLVRTSDKREKKLMD
                     FHDQSKSESNKREEWLKGHLDKNTEQLQDISQTIGVVQKEMSYMSDRIGRLEKEEK"
     gene            23814..24149
                     /locus_tag="ACUBV3_000437"
     CDS             23814..24149
                     /locus_tag="ACUBV3_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HHU3846971.1"
                     /translation="MNEFTTQIITASVPIFGIIAGILTHEVKKLLIKKGGEKAVKIAE
                     IVARNAVEAVEQISVEVGIKGQDKLTEAKTAVIDGLSQYNINLTQTQLETFIEAAVKR
                     MNDEWKKGQ"
     gene            24151..24870
                     /locus_tag="ACUBV3_000438"
     CDS             24151..24870
                     /locus_tag="ACUBV3_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000236382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan amidohydrolase family protein"
                     /protein_id="HHU3846972.1"
                     /translation="MVINIEQAIAWMASRKGKVTYSMDYRNGPSSYDCSSSVYFALRS
                     AGASDNGWAVNTEYEHDWLIKNGYVLIAENTNWNAQRGDIFIWGKRGASAGAFGHTGM
                     FVDPDNIIHCNYGYNSITVNNHDEIWGYNGQPYVYAYRYSGKQSNAKVDNKSVVSKFE
                     KELDVNTPLSNSNMPYYEATISEDYYVESKPDVNSTDKELLVAGTRVRVYEKVKGWAR
                     IGAPQSNQWVEDAYLIDATDM"
BASE COUNT         8713 a         3947 c         5280 g         7049 t
ORIGIN      
        1 gtggtgaaaa acatgatatt gctattgtta aatatggtga aaaagattat ttaaaaaata
       61 ctgatttagc atatgttgta tgcgataaga tcgttgacgc agactctata agcttcgttt
      121 atcatggtga atatgaaact tggcatttta aactattaaa cacagaagca aattaaagtc
      181 ccacgcaagc gcctaagagc ctgcaatagc tctgtgggtc tacgagctgg aatactcgtt
      241 aaacttaccc tggaagcttt ctgtaagtat tcagctgcgt agcgtggaat aatcgttacg
      301 tagttataga gcgaaatttt tagaaaggga aatatcctcc gacatttttt tcataaaaat
      361 ctaaagtctg ttatcgctca cagatgatta tacaagcgta atgctgcaaa taaagtgctg
      421 acgcaaaact aaaaatttaa tactcgacat ttaacaacac aaaaaaacag tcagcagaaa
      481 aaggaaagga gaacaataaa aaagcgctcg tgaaagcgcc attcggtata tattcgtaca
      541 actattatat catacgagga gctttcatga cgttttttcc agaaattaat atacaaaaga
      601 ctaaatcaaa cgctaagcga aaactaagag agtatccacg ctggcgtagg atcgctaatg
      661 atgtagatac tcaaaaagtg acagccactt attcctttga gcctagacaa tcacacggaa
      721 ctcctagtaa gccagttgaa cgcttagcac tcaaccgtgt gtcagctgaa caagaattag
      781 aagcaattga gcaatcagtt agtatgatat tggagccaga aaagcgtagg attttgtatg
      841 acaaatacct atctccttac aaaaatgcag ataaggttat ttatacagaa ttatgtatgt
      901 cagagagttt ttattatgac acgctagatg ctgcattatt agcttttgca gagctttata
      961 gggagggttc tttgattgta gagcaaggag tttttgacta gtttttatac agtaatacaa
     1021 tagtttatac ataaaaatat gtgttaatat agtattatca aaatagcaag aagagataat
     1081 catttaccaa caggctattt atttagtcgt caactttaac tactatcgaa cttgctattt
     1141 tgtagctaaa aggcgagata gggtgttgag acgtagctca gttgggggag cgatatgact
     1201 ataaagggtc tggaacgtac gcaggttcga atcctgccgt ctcaatagtg gttatttcag
     1261 ccactagagc aatacagcgg gcgtgggaca tggatcggag ttttaaccgt ttttttgtgt
     1321 agacctagtg gtattaatca cgttcgattc gtgatgggtc tataggctta ctttaaaaat
     1381 aagcactggt atctctacgg ggacctttgc gccaagtaag actaaaccgt tggaacatga
     1441 accgtgattg gaaaacggta gaggtagcgc cttgataatt ggattgtcga cggtctgatt
     1501 atatgtgtcg gttcgattcc gactgttcct atagcgtgta ttggcaacaa ggttcaattc
     1561 cttgtgatgt aagtagctaa atatctaggc gaagtggttc gaatccactc acacgcttta
     1621 ctcggtcatc acattgtggt ggctttttat tgtggagaaa aaaatgctta aattagacga
     1681 aaaaaaataa gaaaaggtaa gccatttgga cttccgtacc aaggtagcaa aaagaaaatc
     1741 agcaaaaaga tagttgaaat aatcaagcaa aattttggca cagacaaaaa aatatatgac
     1801 atttttggtg gaggaggtgc aataactgca gagtgtatgc taaataattt agaagtgcat
     1861 tacaacgatt taaacaatga tgttactgat atgtttaaaa gagttattac tcaagataga
     1921 gagtggttaa aaacactaat catcagccga gaagaatttt taaaaataaa aaccaaaaca
     1981 aacaaaacag ttgacgacaa tattaaattg cttgttaaca gttttgggaa caactcaata
     2041 agttttttgt ataacataga attgtcagat ataaaatata aattagcggt tgagattatt
     2101 aataagcatg atgtatttag tggctataag caaacagaca catacaaaga gacttgtctt
     2161 ttatgcgagg aagaaaagct tggtaaaatc ggaaacttac agcgattaca gcaactagag
     2221 caattgcaac gattacaaca gttagaacga ttacaacagt tagaacagtt agaacgatta
     2281 gaagcgacaa atttagatta taaagcattc tcgaatgttg atggcgctat tttttatctt
     2341 gatccaccgt atgaaatgac gagccagcat tcttacattg gtgatacatt tgacagtcaa
     2401 ttattctacg actgggcgta cgaaatgagc aaggaaaata ttgttattgt ttctagttac
     2461 gaaatatcag atgatagatt taagtgtgtg tatgagttta agacagctag aagcacaatg
     2521 caaggaggaa gtgcttgtgg taaaaccgaa aagttgttta tggtagtgta gctgactgtt
     2581 gagtctattt ttattatgca aaaaagaacc acaacagcgg ctcttatgct tgtaatttta
     2641 attcaagtgc ttcagtaagg acttgagaaa agttgaggtt tttatcttcg gctgcgttgt
     2701 tcaaccactc aggaatagtc acgtttttgc gtaccttctt agagtgatat tttttcatgt
     2761 aggcaatcat atcaatgcca attaaagcaa tatcagaatc aggatactgt tcttttaaat
     2821 cagaaacgga gctagccttt ggataatcag tatagtcttc aagagcaaat cctaagactt
     2881 cgacagccat ttcataggcg tgctttaagt cgttgccgaa agtaatagct tcaggaacat
     2941 ctggaaaacg aacgccaatt gaattagttt cgttgtcgtg tgtaaaaaca gctggataga
     3001 ttaacatagt ttttcctttc taggtaaaca ataaccaaga tggtcagagc ttatttcagc
     3061 cctgccttct tgagaatatc gtcttcggtg ccttttggca tatcaccttt gtgcatgggc
     3121 acgattgtga tgttgcctgt ttcaaagttt ttgtactttg cgtgactacc gttttgactg
     3181 acttttacaa agccgttctt tttcaagagt ttgatgattt ctcttgcggt tcttggcata
     3241 ttgcttacct cactttctat acttatatta tacacacata agcaataatt gtcaagcaaa
     3301 acacgcataa aatacttatt tttttaaaac ggaggaggtg gtggaaaata ggtaaattaa
     3361 cactaaaaca acaaaaattt atagatgagt acatcatctg cgggaatgcg actgatgcag
     3421 cgataaaagc ggggtatagt aaaaaaaccg ctggtcaaat tggcgagcaa aacttgaaaa
     3481 aacttgaaat aaagcaagct atacagagtc gtatgaaagt cttagaaaag agttcgatag
     3541 caacagctga tgaagttctt agagtgttta cacaaattct tagacaggaa ttaactgaag
     3601 aagtaacgga acttaatcag attactggtg aatttgtaac gatagagaag caaccatcaa
     3661 tagcagaggt tataaaagca ggaagcgaat tgatgaaacg ttacccaaca aacctagaac
     3721 tcaaaaagat caaccttgaa attgagaagt tgaagtctca aattggcgga gatgaaggac
     3781 aagatgagaa aatagccaat ttcttgaata tggtcaaagg agctatttca gatggagttg
     3841 agtaagttat acacaagaaa gcaattgcaa gttcttgatt acatttggaa taacgattgg
     3901 tttatctgcg gacttcatgg agcaaagcgt gctggaaaaa ctgttgttaa caacgatact
     3961 tttgtaacag agttatatcg tgttcgtaag attgcagatg acttaaaaat agatgaacct
     4021 atctatattt tagctgggac atcatctact tctatccaaa acaacgtgtt acaggagctt
     4081 tataataaat atggctttga gcctaaatat gacaaacatg gatcttttac attttgtggc
     4141 gttaaggttg tgcaagtata tacggggtct attagtgggt taaaaagagc tagaggtttt
     4201 acatcatttg gtgcatatgt taacgaagct tcacttgcta acgagacagt gttcaaagaa
     4261 atcatttcta ggtgttctgg tgagggtgca aggatagttt gggatagtaa cccagataac
     4321 cctaatcatt ggctcaaaac ggattatatc gactctgacg atgatatgat tattgatttt
     4381 agttttaagt tagatgataa cacttttcta tctaagcgtt acattgagtc gataaaaagc
     4441 gcaacgccta gtggaaaatt ctatgaccgt gacattttag gtaaatggac ggtcgcagag
     4501 ggtgctattt attctgatta tgataaaaac gtccacgaag tcgaagaatt gccaaaaatg
     4561 acacgctttt ttgccggtgt cgactgggga tatgatcact atggttcaat tgtgattatc
     4621 ggagaggaca ataatggaaa ttattactta gttgatggta ttgcagagca atataaagaa
     4681 atagattggt gggtaactag agccaaagag tttatacaaa tctatgggaa tgttacattc
     4741 tgggcagata gtgcgagacc agaacacgtt gctcgcttta aaagagagcg tattaaagca
     4801 agaaatggac gtaaagaagt tgttgcaggc attgaatata tagcgaaatc attcaaggat
     4861 aaaaggttat ttatcaagcg agggtgtatt cctcgttttt ttgatgagat atatcaatac
     4921 aaatggaaac ctaacagcac aaaagacgaa ccacaaaaag aatacgatga tgtgctagat
     4981 gccatcagat atgctcttta ttcacagcat aaagaagata gcataagtaa aaccaacaat
     5041 ttcaatgtac tttatcaagg tcttaaaaac tagataggag aaacaatggc acacatagaa
     5101 gattttattg actcaactgg ggaacataaa cttttagagt tgcgttttca tcgtgagtca
     5161 agaatgaggt atcagataag cgatataagc gctttgtttg atgacaacta taaaatatta
     5221 cttgaatact taaaacacca cgagaacatt caaaaaccac gtatacaaga gttgttagat
     5281 tatgcagaag gaaacaatca cgaggtatct aagtctggtc gtaggcaaga tgaggacatg
     5341 gctgatgttc gtgccataca taattatggc aagtatatat caacatttaa acagggatat
     5401 cttgttggta atccaatacg tgttgagtat gacgatgaag ttaacaacga acttttaaaa
     5461 gagttagcaa aaaagaacaa tttccaccaa ttaaatagac aacttataaa agatttgtct
     5521 aaagttggtc gtgcttacga actcgcttat cgtagtgcag atgataaaac aaaagtgata
     5581 agactagacc cgagagagac ttttattatc tataaaaatg acgttgataa agatagtctt
     5641 attggtgtgc gatactacaa caaaagtcaa atagataaaa cagacaagac ggtagaagtt
     5701 tatacaagtt cggaagttat cttttttgaa tttaatggag aattaacaga gacagataga
     5761 caaccgcacg cttttggtgc tgttcctatc acggaatatc ttaatacaga tgacggttta
     5821 ggcgattacg aaacagaatt atctttgatt gatttgtacg actctgcgca gtcagacaca
     5881 gccaactaca tgcaagactt gtcagacgct attctagcga ttattggtcg tgtgtcgttt
     5941 cctgaatacg tagatacgca agaaaaagcc attacatatt tgagagctat gcgaaaagct
     6001 agattgttaa atctagaacc acctgttgat agcgaaggac gtgaaggttc tgttgacgct
     6061 aagtatttgt ataaaaaata tgacgtacag ggaactgaag catataaaaa cagaatcgct
     6121 gaggatatcc acagatttac taatacgcca gatatgacag acaataaatt tgctggtaat
     6181 cagtccggag aagcgctcaa gtggaaagtt tttggactgg atcaagaacg tgtggattta
     6241 caagcgttat ttgagcagtc gttgaaacgt agatataagc ttattgctca tgttagtgag
     6301 tttttaagtg aaattaaaga ttttgatatc agcaagctaa aaataatctt tactccaaac
     6361 ttacctaaat cagaccaaga aaaaataaat gattttaaag cgcttggtgg agaactatcc
     6421 aacaaaacta agatgtttgt cactggtatt gttgatgatc cagacgaaga agaagataaa
     6481 atcatgcaag aatcgcagtc gggcagtttg ctagctcaaa aactagaagc tcaaacacgt
     6541 atgtcagaca aggagttagc tcatggacat gcacactaag gaaggcaaaa gttattggcg
     6601 caaacgtgta aaaaaagaga tggaagccaa agacaaaaaa gatgtcagtt taggaaaatc
     6661 tatgaaacaa atacacgact atcacttccg tgagatagaa aaagaaatag agtcttttta
     6721 tcaacgttat gcagataaag aagcgataga cttaaagctt gctaaaaagg ctgtttctga
     6781 cgttgatata aacgcttatc agaaaaaggc aaaagaatta gttgcaaggg ctaacgaaat
     6841 gcgcaaagag ggaattaaag tcactaaagc taattttacg catcaagaaa atatagacat
     6901 ggctgtttat aacttaaaga tgaaagttaa cgcattagag cttttgcaat taaatattga
     6961 tttagcgatg caaaacttat cggaagatga atacaaagcg acaaaaagat tccttgaaga
     7021 tggttttgaa gaagagttga aatttcagtc gggcttactt ggaacatctg tttctagtca
     7081 aaatgacatt aaaaatctag ctaaagcaac aataaacgca aatttcaaag gcgcaacgtg
     7141 gagtgaaaac atttggcaaa gacaagatga ccttagaaaa atagtaaaag aggaagttta
     7201 taaagctgta acaaaaggcg ataacgctat taagttgtct aataagctaa aaaaagagtt
     7261 tgaggtatca gacagctacg ctagacgtct agcgattaca gaacatgcaa gggtacaaat
     7321 ggaagttagc aaaatatcaa tagaagataa cggatttaac ggctttgaga tattacccga
     7381 gccctccgcc tgttctattt gtaaaggaat agcaagtgac ggtccgtatc ctatggaaaa
     7441 atgggataca ggcaatacag cgccaccatt tcacccgcat tgtcgttgtg cagtcgttgg
     7501 tgaagatatc gaacataaga aaggtaaaaa atgaacaaac gcattaagaa aaaacgcaaa
     7561 ctagaatact atatcgcttc actcgtcgca gaaaacgtta tgttttcaaa ggaattaatc
     7621 aaacaacatg aacggattga acaacttgaa aaaattgtag agcataacgc acaagcgact
     7681 aacaatgaac ttagtcgcat caaaaaacac tcaaagaaaa aatggaaaaa ataagtagta
     7741 acaaaaccag ttgaggctgg tttttattat gcccaaaacg tgcttacggc tataaactgt
     7801 gcaagattta atagtcagac atgactttaa acaggaggcg cctcatggca gatttagtta
     7861 acaatggtgt agtagacgaa gtaacacaag aggaagtcga aactcaagaa gaagttaaag
     7921 cggaagctac atcagagaag acttttacac aagcggaagt aactgaaatg atacaacaga
     7981 atgttaatcg tgcagttgca aaagctcaca aagacgctca ggagcaattt aaagcagagc
     8041 aagacgaagc taaaaaatta gcaaagatga acagcgaaga aaaagctaac tacgaaacac
     8101 agaaattgtt agaagagttg caacaactaa aagatgataa gacacgcaac gaactaacag
     8161 cagttgctcg taaaatgttt tcagaggctg acattaatgt tgatgatgat gttcttagtc
     8221 ttgcagtaac tttagatgca gagcaaacaa aagcaaatgt cactaagcta gctagcgcat
     8281 ttgctaaagc aatcgctgat gatcgcaaat cattggcacg acaagccacc ccttcaattg
     8341 ggagtggttc tattacaact caatcaaatt ttggtgcaag cttagcagaa cgagctggaa
     8401 aagttaacac caaactattt taggaggaag aaatgaataa acgtacagta aaaacatcaa
     8461 aagagatttt acataacttg ccatacgaag caatttctgt aactttagat gcgaatgcat
     8521 ttggtaaagg attggcatcc gctggaacag ttttagcagg tgatggggca tctgttttta
     8581 aagaccgcac caaaaaagta aaaccagttc gaaatgctga agtttctggt gccgatcata
     8641 ttgatggcat tttgttaacg gatgtagatc tgtctaaagg tgacgctacg ggtgcacttg
     8701 tttatcgtgg tacagtaaac tctgataagt tagttgacca agagttagct aaagacacat
     8761 caacactagc tactaaatta ccacacattg tatttgtaaa aggaggaact aaataatggc
     8821 attaatttat gacgttgtaa catctgctaa catcaaagga ttttatgata aacaacaagc
     8881 aaatgttgac ttgactttgg gagaaaaagc ttttccatct aaacaacaac ttggtcttaa
     8941 gttatcattt atcaaaggag cagctggtaa accagttagt atcaaagcgg cagcgtttga
     9001 cactaaagtt ccacttcgtg accgcattgc tgtagtctta ttagacgaag aaatgcctta
     9061 ctttaaagaa ggtatgcttg taaaagaggc tgaccgtcaa caacttaacg ttttagcgca
     9121 aactaaaaat caagaactta ttgacacagt gttatcaaca atctttaacg atgaaactac
     9181 tctaatcgct ggtgctaaag cacgtcttga agctatgcgt atggaagtgt tgtcaagtgg
     9241 taaaatccac atcaattcaa atggtgttat gaaagatatt gattatggat taactggaac
     9301 tcaaacgact aagagtgaac aaaaatggtc agaaaaagac accgctaacc ctcttgctga
     9361 tatcgagaaa gctattgaaa cagtaacaga gcgaggtcac gttcctgaag ccatcgtctt
     9421 aaactcaaaa acttttggct atatcaaaaa cgcaaaagca accgtaaaag caattaaacc
     9481 acttgcaccg gaaggctcaa ttgttactaa agcagaatta aaatcttatc tttctgaaga
     9541 attgggatta aatatcttac ttaaagatgg tgtgtttgtt aatgacgcag gtgaaagcaa
     9601 gaagtatttc cctgatggcg tagttacact tgtacctaat ggaaatcttg gctatacagt
     9661 attcgggaca actccagagc agtctgacct tatgggtggc caagcaactg atgcacaggt
     9721 atctcttgta gagacaggta ttgctgttac aactactaag actactgatc ctgttaacgt
     9781 acagactaag gtttctatga ttgctctacc atcattcgag cgcttagatg aagtacagat
     9841 tgtaacaagt tcggaagtat cattataaaa ggaggtaatc gtggctctag taattgaagc
     9901 ttttagagat aaagagactg gttttattta taaagtcggt gaagagtata acggtgctag
     9961 agttgagttt ttgactggta aaggtgtttt aaaagctact gatacacagt caacaaactt
    10021 tagcaattta aaagttgacg agctaaagcg tggacttgat gaactaggtg ttaattacga
    10081 ttctaaagct aaaaaagcgg aattgctaga gcttttagaa tctcacaccg attaattttt
    10141 ggaggtgttt atggaaaaga taaacacgca aacaatcatt aataatgtaa agcttgattt
    10201 agatatcaac gatacattac aagacaaact attggaaatg ttgctaaaaa ggattactga
    10261 ccacttttca gcagagtatg gcactaatga gatagatagt gccttttcgt ttgtccttga
    10321 agattgtttg attgctcgct ataacaggcg aggtgctgaa cgggctaagt ctgagtccgt
    10381 ggaaggaaga gctatcactt attacgactt tttaaacgag tttgaaccgt atgatttaat
    10441 gattaaaagt aagcttaata tttcaaatca aaaatctaaa aaaggtggac tttatttttt
    10501 atgagatata acgatagagt tactctttta attaaagcta acggtgaacc tcgatatgat
    10561 ccaactcttg ggaaacgtgt tggaggagaa gttaaaaaag atgtcgttcc gtgtaacatc
    10621 tcggagcaag gtatagaact atctaatcta ttagacgaga agttagacct tgatagacgc
    10681 attgtaaggc tacgtcacag cgttaagaag gtggataggg ttttaatcaa agataaagct
    10741 tatcgtgttg taacgagcag aaataaagct ttatacgttg aggagattgt taatgattaa
    10801 cttaacattg gaaggggaac atgagttgct atctgcgtta aaaaaagaag ttacttttga
    10861 aaacaagcgc aaagctgtca gaaaaaacgg tcgtaagcta cataaaaaag ctgttaaaaa
    10921 cgcaaatttc aacggtcatt ataaaaaaag aaaaggcgag gaatatgtat ttgttaaacc
    10981 tacaggagct actagaaggt ctattaaatt agagtttagt aatcaaagta cagttgctaa
    11041 agtgaaggct ggaacaaatt attccggtta cttggaaaca ggtacacgat taatggagga
    11101 gcaaccgttt atgaagccag ctcttgatag tgtcattgac aacttcatta aagatttagc
    11161 gagggttgaa tgatgaaaca accggatcaa caattgtttg atgagatttt taaaagactc
    11221 actgatatag gattgactgt ttatgatttc cttccaccgt tgggtacaaa ataccctttt
    11281 gttgtgatgg gagacactca tataattcct atggctacaa aaacgcagtt aataggtaag
    11341 tgctcaacga ctatcaatgt ttggggagat ggaacaaata gaaaagtagt aagcgatatg
    11401 attgctcaaa tcatgttgga agtcagtaag attagccaaa ttgatggtaa caaatgggct
    11461 atggtaatga acgaaagcga tactcaaatc ttaaaagata acagtacaaa cgaaaatctt
    11521 tatcatggca ttttaaatat ttattttaat tttgtatagg aggaaatatg gtcacaagtt
    11581 caccgattta tgggaaagat aatattttga tgttccgtgt gcttggagat aaggcagctg
    11641 cggctaaatt gtcttttcag acagaacata aatggaaata tagtagaaaa acagatgcta
    11701 aaatcacaaa agatggtgct atcaattctg acaaagggct tgaggtaact ttggaaatca
    11761 aaggcgtggc aagtcgtgat gaattgaata cgacactaaa gaatgctgtt ttagaaggta
    11821 aacaaattga agtttgggat attgacttga atagcaataa cgattcagac gggaaatatg
    11881 atgcagaata tgctattggg cgattgggat cttgggaagt cccttccaat gttgaggaat
    11941 tttctgaaat ttccacagaa atggctatcg atggcaaacc agttaaaggt aaagccactc
    12001 ttacaaaaga acagattaaa gctattcaat atgtatttaa agatgtaaca aaaattgatg
    12061 aatcttccgt ttctagtccg ggttctgttc atagcagata acatagattc taaatagcaa
    12121 aataacacta gggcttttag ccctttttat tttttaagga gcaaaaacaa aaaatgaaag
    12181 aaattacaat ttcaggaaaa aattacccat taaactttgg ctttgacttt attcgagaaa
    12241 tggataagcg cttttttgtt gataacaatg gctttaagtt tggtacagga gttcaaactg
    12301 cagttatgca gctagctgca aaaaatccgc ttattttaga agatattatt ttatcagcta
    12361 cacacacaga atactcaatc cctaaaaaag aagatattga aaagtgggct atagaacaag
    12421 cagaaaatgg aaaactggga gaagttttag atgatttttt aacgatcttg aagaaagcac
    12481 ctcttttgaa aacacaagta agcccaatct taaaagcttt agaggagggt taaatcaaaa
    12541 agatataaca agttcttctc ttgaaaccta caacgagatt attagcaaca tttttgggtt
    12601 gttggatgtg aaagacttta atgttgctcg tcgtatgaca gtaaaagaat acaatttaag
    12661 gattaaaggt tatcttttaa aacgtttaga atctgaaaga gatatttact taagtgcttt
    12721 tgttaatcgc aacgttaaag ctgccaattc taaaggcgaa tatgtttacc gtgaatttga
    12781 agagttttac gattatgaga aacggaaaaa gcaagtattg tcagcatcgg ttgataatac
    12841 cataaatcct aaaatcattg acagagctaa tagagttaag gaattaagag agagaggagg
    12901 tttaaatgtc tagtagagca tacacagtac aagcgatttt aaaagcaaca gatactaatt
    12961 ttacaagcac tatgaacaaa gttcaatctg ctgcacaagc tactattgat aaaataaaat
    13021 caataaaaga tagcaacata tctacgcttg ggaaaatcgg cgaatatacg actatggtag
    13081 gtcaagggat gcaaagtgtt gggcgtagcc tttcaaaata cgtcacactt ccaatagttg
    13141 ggttaggagt cgctgctgca aaaacatttg gagattttga atctcaaatg aacagagtaa
    13201 aagcaatctc tggagctacc ggggcagatt ttgaaaagtt aagaaaacaa gctattgatc
    13261 ttggggcatc gtctgttttt agtgctaagg aagttgcaca aggtcaagaa atgatggcat
    13321 cagcaggatt taacgcaaat caaatcttag cagcttcacc cggagtaatg tctttggctg
    13381 cagcatctgg tggagatcta gctcttgcct cagaagcggc agcgactgcg gtaaatatgt
    13441 ttagcttgaa cgcaagtgaa gccactcatg tagccgatgt gttcgcaaaa gctgcagcag
    13501 atacaaactc agaggttgca gacatggcgg aagcgttaaa atacgcagga ccagttgcag
    13561 gagctatggg aatttctatg gaagaaaccg cagcagcaat aggtattatg tcaaacgctg
    13621 gtataaaagg ctctcaagct ggtacaactc ttagaacagc tatcaccaga ttagcaaaac
    13681 cgacagacca aatgcaagct gttattgatg gtctcggatt gtcttttttt gatacgaatg
    13741 ggaagatgcg gtctttgaca gagataacag gacaactaag agagaaaatg tctggattga
    13801 cagatcaaca aaaatctgca gctttatcaa ttttatttgg taaagagtct ctgtcaggga
    13861 tgcttgcttt aattaacgct actccaggcg agttgtctaa attaaccgaa gggttgaaaa
    13921 attcaaaagg tgctgctgac aaaatggcaa acactatgaa tagcggtctt aaaggagcaa
    13981 ttgaacagct caaaggttct ttagaaacag ccggcattac aatcggtggt atattaaatc
    14041 cattgttgca aggagttatt ggtaaaatcc aagcagtcat agattggttc aataagttat
    14101 ctcctgctgg gcaaaagctg gcagttatta ttggaggtat tggcgcagct cttgggcctc
    14161 ttttggtcat ttttggaaca gtaattattt ttattggtca agtaactgct tctttagaag
    14221 gagtttctac agcgttcacg gcaatagcag gaatcttttc aataatttca ggacctgtaa
    14281 ttttagttgt tgcagcaatc ggagcattta tagctgcact tgtagtcgct tggaatacat
    14341 ctgagacatt tagaaataca attatttctg gttggaaatc aatggttgat gcagtcaccc
    14401 cgctaatcga aacgttgaaa caactaatat tatcaatttt cactttcata caggctaaca
    14461 gcgctacttt tattggcggt ttaaagttag cttggagtgc gcttgttgaa gctctaagtg
    14521 gtttgtttct ggttataaca ggcgtggtac aagttggcat gtctgtcatc aacacaacta
    14581 taaaagtgat attagcaatt ataaatggtg attggaatgc agcgtggact gctatcaaat
    14641 ctggagtagg agcaatttgg gaaggtataa aaaacataat aattgggggt ctgacgtttt
    14701 tagctagttt atttgtaagt atgctgggtg ttttcgtatc agttttttct gctggatgga
    14761 gtgctataaa aggtatattt tctgtgacgc taaaattttt ggttaacgta gtagctgctg
    14821 gaatgagtgc tattggaaac gagatatcat ctaaaattaa tacagcaaaa agtgtcgcta
    14881 tactagcatt taatgcgatg aaaaacggta tttcaacagc tattaatgct gcgaaagaag
    14941 tcgtttctag tgtagttaac cgtataaaaa acattcttaa ttcccttgct aatattaact
    15001 taggagcagc tggtagagct ataatggatg gatttttaaa tggattgacc tcagcttttg
    15061 aaaaagtcaa aaattttgtc ggtggtatcg ctggatggat tcaagagcat aaagggccaa
    15121 taagttacga tagaacatta cttattccag cgggtagagc tataatggga ggttttaata
    15181 aatctcttac agatagcttt gaaccagtca agaaaaatgt ttctagtatg gctagtcgta
    15241 tttctgagga gtttcaaaac ggattgaata aacttaaaga tatcaacata tcggaaatta
    15301 cggaaggttt acaaagtagt tttgacgtga ctcattatgc aagttttggc ggactaaatg
    15361 actcagcaag aaaattagaa gatgatagat ttgtcgcatt gaaagatgcg gttttagcta
    15421 ttagagattt tgctaataga gacgttgtag tgactgtgaa tggtaaagaa ttagctcgaa
    15481 cagctggaga caacttcaca gaataccaaa aacaaaaaga aataatgaat aatagaatga
    15541 aaggtttgat ataattggca aattttagtt ataaaggcgt tgatttatcg ccttttttga
    15601 attgtttaaa catagtgagg actataggta ataataggtc tattgctacc agaaaaataa
    15661 atgaattagg agaagccatt caatcggtta gttttggagc taaaactatt tttgttactg
    15721 ttagttttaa aactaaagaa atcggagcaa gtaaatttgt agacacgaca gaaccgtcga
    15781 cttatagtta cgaaaattta aataaattaa gagaaaaaat agctggtatt ttgcacagta
    15841 agacgacctt taaacttaca ttaccagatg agcctgacag atattatatg gctgttccaa
    15901 aaggtgatat tgacttaaaa ggaatatctg attggtacga cgaaacggtt attgagtttt
    15961 acataccaga cggcgttgca cattcgacta cttacaaaaa gtttttagat tacacgcgag
    16021 atggaaataa actaaccttt aaattgcaaa acgaaggtaa cacaaatgcg ttgccaatta
    16081 tcaaaataaa acacaactcc gaaaacggct atattggtat tgcaaacgaa acaggtgctt
    16141 ttgcgcttgg atcatcagaa gaagaagacg ggactatcgt gcatcgtaac gaagtccttt
    16201 ttgattactc aaaagcgata gcgaaagctt tggagggtgc gccaaacgtc gcaaaactta
    16261 atcacatgcc accttctttc gatacagaac ttaaacgtat gcgtattgat aacatcttag
    16321 gttctggcaa aggcggtgaa tatgttgtta ttggaaatag aggtactact cctggctaca
    16381 cagagcacgt tgggactcga acgtttatta tcaatcctga ttcaaatggg gaatatactc
    16441 tcaatgaaca tttgtggtgg caacagattt ttattgctac tgcgcaggat caaaaaggtt
    16501 ttttaaagct ttgtgtaaca ggaatcgatg atgaaggaaa tgacgagttt ctgtatggaa
    16561 tcgaaactta caaacgaaaa aatggttttg aagcagaata caatttcttt gctctggatg
    16621 atgacggtgt tggttggaga ttttataagc agtttgaatt tcaggcagat agaaattatc
    16681 acaatccttt ttcgatggat agaagcagag ctgttgagat tttcagagaa gaagataagt
    16741 ttcgtattta ctttaacggt gcgcatcatc atgcaactgt tccatctctt aaaggaaaaa
    16801 aatctcgcaa gatacatctt gcaatgggga catgtagtga tagctctaaa tatatcaact
    16861 acaacctgtt tgaaaaagtc aattttgaaa aaatgggagt gtctcattac aacaatatcg
    16921 tcaataaata tcaaccaggt gacgaagtta ttattaattt tgaaaatgat acagtcaaaa
    16981 ctaaagatat tgactccctg caggatatgg ttttaggctc tcaaccaata tctattccac
    17041 cgggtgagtc tgaactcgtc atcaatgtgt ctaaattttc ttcaacagac cctgacatcg
    17101 aactattgtt agaagagagg tggttgtaat aactctagta atacacgacg caaaactaca
    17161 tccagttttg cttttagaca atgagcgaca aggagcactt aattattatg atgatttgtg
    17221 gactagacag ctcacaactg gttcgtcagc atttgagttt tctgtttata aaaaatcgct
    17281 gttgggtgat aatccactta atcacaaata tcacgcacta aacgatcaag catttgtttc
    17341 ttttgtacac aaagataaag tacaattgtt taacatcatg cgagtcgagg aaacagagac
    17401 aacaatacat tgctattgcg aaaatcttaa tttagagtta ctaaacgagt attgcaacgc
    17461 atataaagca actaaagcaa tgtcatttga agagtatctt gtgcagtttg atattttaaa
    17521 ttggggtgct ttgacaattg gcacaaacga agttaaggac aaaaaactga cattggaatg
    17581 gactggtcaa gacactaagt tagctcgtat tttgtcaatt gctaataatt ttgatgcaga
    17641 aatcgaattt gaaactcaat tacacaacaa tcacacgttt aaagcgttta ttgtaaatgt
    17701 gtacaaggaa tacgaagagg gcgtgtccta tggcgtaggt cgtgaccgca gcgacatagt
    17761 gttgagatat caaaaaaatg taactggtat cactaaaaaa ttagacaagc gccagattta
    17821 caacgccata cgcccgtatg gcaaaaagac agtcaaaggc gagcgcgtta tttctaatcc
    17881 tgtaactcgc aaagtcacta aaacagttgg gtcaaatcgt acatatttag gcggagacct
    17941 caaatattat ggtcatacaa tcaaaaaagc taacgtacaa tctattatta actacgcggt
    18001 gcagtataat attttgccga gtggaatcat atgtcaactg tacttagaaa gtctttgggg
    18061 tgattcagca gttggtaaac gtgacaataa ctgggcaggt ataagcggcg gagcacagac
    18121 acgccctagt ggagtaaaag tcactactgg aatggctcgt cctcccagcg agggcggaac
    18181 gtacatgcac tatgctagtg tagacgactt tttaaaagac tacacttatc ttttagcaaa
    18241 acaagggatt tataatgtcg tcggcaaaaa gaatatagca gactatacaa aagggctttt
    18301 tagagctggt ggagctaaat atgactatgc agcagcagga tatcaaagct acacaaattt
    18361 gatgactaat atccgaaatg gtatcaataa agtaactgga aatatcctca atacgattga
    18421 taagctgtgg caaacaccag tacagcctat aacagccgta aacgtagcta gaagagctac
    18481 taagacaatg caagcactaa atgaagctac tagacttaaa ggtcgcagaa tcggctcagg
    18541 acagtgttat gctttgtctg gttggtacgc taagaagtta gacggcgctt ggattgacag
    18601 ctcggttggt ggtattagag gtcgtatcgg aggcggtatg gctgctgcct taatcggcac
    18661 tgattataac tggggtgctt atggttggaa gctagacagg tcgcctaatg ctggcaactt
    18721 gcaagctggc ggtatctata atgttaaagc aaattttggt gctccatttt atacaacaca
    18781 atgggggcac acagggatta tcaagagtgt gtctaaaaca agagtcactg tcttagagca
    18841 gaattacgct ggacgcatgt atgtcatgga aaactcgtat gagattaacg cttttgctag
    18901 aggattgcag acagtatgtt atccacgtga aatagcgcaa ggaatggctg ttaacggtgc
    18961 aacaacacag caagtaagcg gtggaacaca aatatcatac gaggaagtcg tgcaagaagc
    19021 acagacagaa tcatacgaag aagaacaaat catctacatt gacaactcaa tctacaaaga
    19081 gtggaaagac gaaaacggta aagtagagta ctatctcaaa aatggatttt tgtacgcacc
    19141 tttatcaaga gaccgttatc catctgtttt aacaggtaac gagacacgag ataactggat
    19201 acgaaaagac atggaagttg agactgatag tcaggatgtc ttgatatcaa ctgctttaaa
    19261 agatttaaaa gcacacgctt atccagctgt cacttatgaa gtcgatggat atgttgattt
    19321 agaacttggt gatgttgtgc gaatacagga cgacggatac gagccaccgc taattctcac
    19381 agcgagggtt attgagcaag aaatatcaat aacaaatccc agctctaaca aaactaaatt
    19441 cagcaatttt gtcgaaaaag aaagtcagtt agcttccgac ttaattagtg atatgttgcg
    19501 tctatacgat gagtcaattc catacgatat acaactagcg acttcaaacg gagttgcttt
    19561 taaaaatggg gttggtgagt ctgtattaac gcctaacctg caaaaaaatg ggaaagatta
    19621 cgatgctatt tatttttata aaaatggcga ctcactgatt gagataggtc cttcgctaac
    19681 agttaaagca agtgacttta accatgtttt aaacataaca gtcgaagctt atgttaacga
    19741 ggaacttgta gcaagtacgc aaatatcctt tacagatact gaggatggag aaaaaggcga
    19801 tgatggtgct acatcatgga cagcgtgggc caattcgaaa gatggaaaag ttgactttag
    19861 tattactgaa gctaaaaata gaagatttat cggtacttat actggattaa cgcaatcaac
    19921 aaattatctt gactacaagt ggattgatat gtctgctaat gttgtcattg gtactcaaaa
    19981 tttacttgat ggtacaaaat cattttctgg aagttggttt accgaaggta caatatttga
    20041 gactacaaaa atcagcgaat atccatttga atttaagaaa tggaagtctg gaaataaggt
    20101 tagtcacact atcgagtttg atgttaaagc tggtgtaaca tacactttta cagctgctat
    20161 agcaagagaa aatgctggaa gattgtactt ctatttgtat gacttgtttg caaaccatat
    20221 cacaagtaac acacctcgtg agacgataat tgaaaatgtc actacagata tccagatgtt
    20281 taaagttaca tttgtaccgc tcagagacgg taaaataaaa ccacgctttg ccatgcttgc
    20341 cagtgatgca ggttggttta tgactggtgg atatatgctt gtcaaaggta ataaatctgg
    20401 agattggcaa gagtcagaag ctgataaagc aagtaatctt gattcaaaag ctgatcaaga
    20461 attaacccga gcacaaattc tagctcttga agaaagaact gctatagcaa gagaaaatgc
    20521 aattgctgag gctatgcagc atacactcag cgaagttgaa actaagtgga agctttggta
    20581 tgacttaaat acgatagacg aaaagcaaaa agttgcaaac gacattgctc aattgtttga
    20641 tcgtacaact gagtttaaac aacttttagg tgaggcaagt gcaaaattta gctttatcaa
    20701 caacgagaca ttgattggtg aagaggggat tgccattggt gacaaagatg gcaaagctaa
    20761 gttattccta tcaaatgaca gcatctcctt tgttactaat ggtgttgctc aaatgacact
    20821 aacaggtgat accttaacga taaagaatgg tctgtttaca gagcgtatac agattggaaa
    20881 ttttgttgaa gaagtctatg acagaaatcc attatttaat gtaatcagag cgattaggaa
    20941 tagttaggag gtgagaaatg ggaacatcaa catttagtgg aacttggggt aacaatctaa
    21001 cattagatgt acgaacaagc tatacacaaa atttagttgg taattacagt acggtcacag
    21061 ttaaagtcta cgaaaaaatt agctcctatg gttatattga ctaccctggc gaacgtacaa
    21121 tgacaatagt tgttgatggc aaatcttata atgagagtgt caacgtagac attaattatg
    21181 gtcagacaaa agaattaggt acatatagct accgtataaa tcataactct tatggatcaa
    21241 aaccaaaatt taatgttacg gtaacgctgc ctatcaattt tagcaactat gggtcggcat
    21301 cagtaacaaa ttcagttagc ttaccaacaa taaagcgtgc tagtacagcg acaatagcaa
    21361 gcggaaatat tggtagtgca gttgctatta ctatcaaaag acaatcagat acttttagac
    21421 acacattgaa atttgatttt aaaggtctaa caggcacgat tgcatcttta gttgacacat
    21481 cttactcatg gacgttgcct gcatcactgt ctgtcttgat accaaatgac cgaagtggta
    21541 caggaacttt gattgtagaa acatttacaa gtgatagtca aaaaatcggt gagaacaagt
    21601 atacctttac tgcaacagtg ccagacattg ctgcatataa gccaatattg gcaagtatta
    21661 gtttatctga tgctaacact ctgacaggta gtttgataac tggcaataac tatgtcagga
    21721 acatgagtaa attaaaggtg tcttttggta actcggcagg tgctaatggt tcaactatta
    21781 gctcatataa tgctgaaatt gttgaaaaag gcaaagctat ttttggaaat ggtagtgttt
    21841 ttgatatgtt ggattttgtt ggaacagcaa caatcagagc tacggtaact gatagcagag
    21901 gtctaacatc agaaccagtt gacacaaaaa ttaatgttat tgattatttt ttaccaattg
    21961 ttacaagtgc aaaagtagtc aggtctcagc aaaatcctga tattttacaa gtcttgccat
    22021 ttgttaagat tgcaccaatt atagttggtg gaatacaaaa aaaccaactc aaaatgtcgg
    22081 tatctgttgc accatacaat actggtatct atgcagttga tagtggcgca gctacaaata
    22141 cctggtcaac aatttcccaa atgtcaggtg cccctttaaa tcttggcggc acttatgaca
    22201 agtcaaaatc atggcttgtt aagatttcgg tgagtgataa cctaatgtca gcaacaccaa
    22261 taatccaacc agttgcaagt gagtttgtac ttgtaactaa agcaccttct ggtgttgcat
    22321 ttgggaaaat ttgggaacat ggtattattg atgccaaagg cgatgtttat gttgatggta
    22381 atatttattg tggcgataag ccaatccaac aaaaacaact cgctcttaac aacggcggtt
    22441 cttttagaca tgacgacact gacctaaata gcttgcaaga cacaggtttt tattgtgtat
    22501 ttagaggtgc taatagaccg accggagcag gccctggcta tgtaactgtt gtaagacacg
    22561 agacatcaaa ctatgcttat caacaatttt acgaccgtac aaataaaact atatttacca
    22621 gagtattgga aaatggggtt tggagcggtt ggagtgagta tgttaaaaaa gatagtttgc
    22681 ctacaacgat agacacaggt tggcagtcga ttggaaatgg ttttagttat aggcagacag
    22741 gcagtacagt caccgttaag tacaactttg cgacgaatgg catagataag ttgacggttg
    22801 gctctatgcc tacgaatttg ataactagcg acatgatgtt tgcagtaacg gcttggacta
    22861 tacaactcaa tgttttaaat gttcaagtga gcgcagacgg tcgtattctg tggtttaatc
    22921 cgtcaaaatg gactgttaac gtaaaaggtc aaattcagtg gacgatttaa aggaggaaaa
    22981 ctattggaaa ttttaaacaa atatcctgta atgctagaag ataaaagcat tgcaaaagtt
    23041 aatgcaattg tggcagttga tttacctcac gtaagaggta acttaacttt tgacttacca
    23101 gttgactttg ataataaatc ttttgcagaa acgcttgaaa agtgtgagca gatattttac
    23161 gacgaaaagt ataaagataa agctcagtct gaaaaaatga ctgaactaag tacatcaaca
    23221 tcaacaggca cacaaacact tatcaatctg ataagtacgc tttacgcaaa agaggtttta
    23281 aaagatgaag atcttattgc tattggttag aattttttta caggaagaag ggatagatat
    23341 gatgattaaa ttatttgcga ttgacttata ttatggacgt atggcttggt caagttttgt
    23401 taaaaaggga ttttcagagt ttattaataa caaaacaaaa gagcaacttg caattatgtg
    23461 cgatgaagaa ttacttgctg aaattttagc aagttagtga ggtagtcgga tgacagtaga
    23521 acaagcagaa agaatcgctc aatcacaatt tgtgtgggct attctcttta tcttgctttt
    23581 tatgattgtg gttggttatc tggtgcgaac gtctgataag cgtgagaaaa agctaatgga
    23641 tttccatgac caatcaaaat cagaatctaa caaacgtgaa gagtggctca aaggtcactt
    23701 agataaaaat acagaacagt tacaggacat ttctcagacc attggtgttg tccaaaagga
    23761 gatgtcttat atgagtgacc gcattggtcg tctagaaaaa gaggagaaat aagatgaacg
    23821 aatttacaac acaaatcatt acagcatcag taccaatttt tggtatcatc gcaggtattt
    23881 taacgcatga agttaaaaaa ctacttatca aaaaaggagg cgaaaaggct gttaaaatcg
    23941 cagaaattgt cgctcgtaat gctgttgagg cagttgaaca aatatctgtg gaagttggta
    24001 ttaagggaca agataagcta acagaagcta aaaccgctgt tattgatggt ttatcgcaat
    24061 ataatattaa tcttacacag acacaacttg aaacttttat cgaagctgcg gttaaacgca
    24121 tgaatgatga gtggaagaaa ggtcaataat atggtaatta atattgagca agctatcgca
    24181 tggatggcat ctagaaaagg taaagttact tactcgatgg actatcgaaa tggtccttca
    24241 tcttacgatt gctctagctc tgtctatttt gctctacgct cagcaggcgc atcagataat
    24301 ggctgggcag taaatacaga atatgagcac gattggttga taaaaaacgg ttatgttctt
    24361 attgcagaaa atactaattg gaatgcgcaa agaggtgata tttttatttg gggtaaacgt
    24421 ggggcttcgg ctggagcttt tggacatact ggtatgtttg ttgatccaga caatattatt
    24481 cactgtaatt atggttataa ctcaattaca gtgaataacc atgatgaaat ctggggatat
    24541 aatggtcaac cgtatgttta cgcttatcgt tactctggga aacagtcaaa tgctaaggtt
    24601 gataataaat cagttgtatc taaatttgaa aaagagttag acgttaacac accgctaagt
    24661 aattcaaata tgccatatta cgaagcaaca atttcagaag attattacgt tgagtccaag
    24721 cctgacgtca atagcactga taaggagttg cttgttgctg gtactagagt tcgagtttac
    24781 gaaaaagtaa aaggctgggc aaggataggc gctccacagt ctaatcagtg ggtagaagat
    24841 gcttacttaa ttgatgcaac agatatgtaa accaacagag cgatataaat gtcggtctgt
    24901 taatggtgta agttacaccg caactaaaaa aacaatttag gaggtaaaat tcctttagat
    24961 aagacaaatg ccctcgcttt tgcgggggc
//
LOCUS       DBJYSH010000013        24285 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.013, whole genome shotgun sequence.
ACCESSION   DBJYSH010000013 DBJYSH010000000
VERSION     DBJYSH010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 24285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24285
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.013"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            103..906
                     /locus_tag="ACUBV3_000439"
     CDS             103..906
                     /locus_tag="ACUBV3_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HHU3846973.1"
                     /translation="MTIYDQIESALDLMTDLEREIACYFMGQPISKDALASTIVTKQL
                     HISQAALTRFAKKCGFKGYREFVFEYLKSHETISQQLYGLQNDNTKKVFMNYQEMVSK
                     SADIIDEEQLLEVSHMIEQADRVYFYGKGSSSLVAKEFKIRLMRLGVICEALDDTDSF
                     SWTNSIVNDRCLVIAFSLSGNTNSVIGALKIASSHGAKTVLFTKQPHTIDYAFDKIIQ
                     VASARHLDYGNRISPQIPMLIMVDIIYAQFLDINKIEKERIFRETIIQR"
     gene            complement(929..1906)
                     /locus_tag="ACUBV3_000440"
     CDS             complement(929..1906)
                     /locus_tag="ACUBV3_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HHU3846974.1"
                     /translation="MIETMSLDDMREYLGQDQIPEDFDDFWKKQTMKYQGNIEYRLDK
                     KDFNITFAQAYDLHFKGSNDSIVYAKCLFPKTNKPYPVVFYFHGYQNQSPDWSDQLNY
                     VAAGYGVVSMDVRGQAGQSQDKGHFDGITVKGQIVRGMISGPNHLFYKDIYLDVFQLI
                     DIIATLESVDSNQLYSYGWSQGGALALIAAALNPKIVKTVAVYPFLSDFRRVLDLGGV
                     SEPYDELFRYFKYSDPFHKTENSVLKTLAYIDVKNFAHRISCPVVLLTALKDDICPPS
                     TQFAIFNRLTSTKKHLLLPDYGHDPMTVQVKDYIFDQLTGSQFTKQKIE"
     gene            complement(1914..2795)
                     /locus_tag="ACUBV3_000441"
     CDS             complement(1914..2795)
                     /locus_tag="ACUBV3_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HHU3846975.1"
                     /translation="MTRTVAIDIGGTMIKHGIVDNLGCIVEASELATEAYKGGPGILQ
                     KVCQIIDNYLTEGSIDGIAISSAGMVDPDEGCIFYSGPQIPNYAGTQFKKVLEDTYQV
                     RTEIENDVNCAGLAEAVSGSAKDSSIALCLTIGTGIGGCLIIDKTVFHGFSNSACEVG
                     YMHLSDGDFQDLASTTALIADVAKAHGDEISRWDGRRIFQEAKEGNEKCIASIDRMIN
                     YLGQGIANMVYVVNPEKVVLGGGIMAQKDYLQDKLSESLKRNLVTSLAEKTAIVFAQH
                     ENQAGMLGAYYHFKNRG"
     gene            complement(2812..3729)
                     /locus_tag="ACUBV3_000442"
     CDS             complement(2812..3729)
                     /locus_tag="ACUBV3_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000654097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HHU3846976.1"
                     /translation="MKDLQKYQGIIPAFYACYDDKGDICPERVKALTNYFIDKGVQGL
                     YVNGSSGECIYQSVADRKLVLENVMSVAKGKLTVIAHVACNNTKDSVELAMHAEAIGV
                     DAIAAIPPIYFRLPEYAIADYWNTISQAAPQTDFIIYNIPQLAGVALTSDLYRKMLQN
                     PQVIGVKNSSMPVQDIQNFVAIGGENHIVFNGPDEQFLGGRLMGAAAGIGGTYGVMPE
                     LYLTLNQLIVDKDLEKARELQFTINDIITKLCSGHGNMYAVIKSVLEINEQLTIGSVR
                     LPLASVTEEDKPIIKEAAEMIRHAKKQFC"
     gene            complement(3726..4388)
                     /locus_tag="ACUBV3_000443"
     CDS             complement(3726..4388)
                     /locus_tag="ACUBV3_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HHU3846977.1"
                     /translation="MKKANQLIAAIFDVNNTFMQGCNVVFDLALLNLLFMITCLPLVT
                     IGAAKISLYRTLWQKLEGDQTNLLILYIKHLKKEWFQGMLLGLVELSILVVIIFDLTI
                     LHYQIGFIVSFLKITCYAFLLLTVMTSIYLFPMAARYEMSLLDTVKKSFIMACLNLKW
                     TGVLMFLLIMTWFIMVQSSLLFMLTVSAIFIFAYTAFAYFKIIILQKQFAYFSKQQGD
                     YQ"
     gene            complement(4408..4851)
                     /locus_tag="ACUBV3_000444"
     CDS             complement(4408..4851)
                     /locus_tag="ACUBV3_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HHU3846978.1"
                     /translation="MIYDHLLNLTHYKDINPNLDLAIDYLLSHDLRNLDIGTYHISPE
                     VILMVQSNQLSESFDHIFEYHKKYLDIHYVIEGHEVIKLGKGDKVEVEEYLGDIGFIK
                     CSEETSFDLRDNYIAFFFPEEAHQPNGMGSLGNYVKKGVLKVLMA"
     gene            complement(4864..5694)
                     /locus_tag="ACUBV3_000445"
     CDS             complement(4864..5694)
                     /locus_tag="ACUBV3_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HHU3846979.1"
                     /translation="MKKKTFSAYNFLTALILCLLTVLFIFPFYWIMTGAFKSQPDTII
                     IPPQWWPKAPTLENFKALTVQNPALRWLWNSVFISIMTMFLVCCTSSMAGYVLAKKRF
                     YGQKILFSLFIAAMALPKQVVLVPLVRIINFMGIHDTLWAVILPLVGWPFGVFLMKQF
                     SENIPTELLESAKIDGCGEIRTFINVAFPIVKPGFAALAIFTFINTWNDYFMQLVMLT
                     SRNNLTISLGVATMQAEMATNYGLIMAGAALAAVPIVTVFLVFQKSFTQGITMGAVKG
                     "
     gene            complement(5704..6591)
                     /locus_tag="ACUBV3_000446"
     CDS             complement(5704..6591)
                     /locus_tag="ACUBV3_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HHU3846980.1"
                     /translation="MRTNKLKMRETMIAYAFLAPILLFFLIFVFAPMVMGFVTSFFNY
                     SMTQFTFIGLANYNRMFHDSIFMKSLINTVIIVIGSVPVVVFFSLFVAANTYEKNVFS
                     RSFYRCVFFLPVVTGSVAVTVVWKWIYDPMSGILNYILKSGHVIEQNISWLGDKHWAL
                     LAIIIILLTTSVGQPIILYIAAMGNIDNSLCEAARVDGANEMQVFWQIKWPSLLPTTL
                     YIAVITTINSFQCFALIQLLTSGGPNYSTSTLMYYLYEKAFKLSEYGYANTMGVFLAV
                     MIALISFAQFKILGNDVEY"
     gene            complement(6679..7995)
                     /locus_tag="ACUBV3_000447"
     CDS             complement(6679..7995)
                     /locus_tag="ACUBV3_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HHU3846981.1"
                     /translation="MSIKKSVIGFCLGAAALSMFACVDSSQSVMAAEKDKVEITWWAF
                     PTFTQEKAKDGVGTYEKKVIKAFEKKNPNIKVKLETIDFTSGPEKITTAIEAGTAPDV
                     LFDAPGRIIQYGKNGKLADLNDLFTDQFIKDVNNKNIIQASKSGDKAYMYPISSAPFY
                     MAFNKKMLKDAGVLKLVKEGWTTSDFEKVLKALKNKGYTPGSFFANGQGGDQGPRAFF
                     ANLYSAPITDKEVTKYTTDTKNSVKSMKKIVEWIKKGYLMNGSQYDGSADIQNFANGQ
                     TAFTILWAPAQPKTQAKLLESSKVDYLEVPFPSEDGKPDLEYLVNGFAVFNNKDENKV
                     KASKKFITFIADDKKWGPKDVIRTGAFPVRTSFGDLYKGDKRMMKISKWTQYYSPYYN
                     TIDGFSEMRTLWFPMVQSVSNGDEKPADALKDFTQKANDTIKKAAK"
     gene            complement(8042..8740)
                     /locus_tag="ACUBV3_000448"
     CDS             complement(8042..8740)
                     /locus_tag="ACUBV3_000448"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047465 - N-acylglucosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HHU3846982.1"
                     /translation="MPHLSKEAFKKQIKNGIIVSCQALPGEPLYTESGGVMPLLALAA
                     QEAGAVGIRANSVRDIKEIQEVTNLPIIGIIKREYPPQEPFITATMTEVDQLASLDIA
                     VIALDCTLRERHDGLSVAEFIQKIKGKYPEQLLMADISTFEEGKNAFEAGVDFVGTTL
                     SGYTDYSRQEEGPDIELLNKLCQAGIDVIAEGKIHTPKQANEINHIGVAGIVVGGAIT
                     RPKEIAERFISGLS"
     gene            complement(8987..10291)
                     /gene="sip"
                     /locus_tag="ACUBV3_000449"
     CDS             complement(8987..10291)
                     /gene="sip"
                     /locus_tag="ACUBV3_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000783421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface immunogenic protein Sip"
                     /protein_id="HHU3846983.1"
                     /translation="MKMNKKVLLTSTMAASLLSVASVQAQETDTTWTARTVSEVKADL
                     VKQDNKSSYTVKYGDTLSVISEAMSIDMNVLAKINNIADINLIYPETTLTVTYDQKSH
                     TATSMKIETPATNAAGQTTATVDLKTNQVSVADQKVSLNTISEGMTPEAATTIVSPMK
                     TYSSAPALKSKEVLAQEQAVSQAAANEQVSTAPVKSITSEVPAAKEEVKPTQTSVSQS
                     TTVSPASVAAETPAPVAKVAPVRTVAAPRVASVKVVTPKVETGASPEHVSAPAVPVTT
                     TSTATDSKLQATEVKSVPVAQKAPTATPVAQPASTTNAVAAHPENAGLQPHVAAYKEK
                     VASTYGVNEFSTYRAGDPGDHGKGLAVDFIVGKNQALGNEVAQYSTQNMAANNISYVI
                     WQQKFYSNTNSIYGPANTWNAMPDRGGVTANHYDHVHVSFNK"
     gene            complement(10438..11337)
                     /locus_tag="ACUBV3_000450"
     CDS             complement(10438..11337)
                     /locus_tag="ACUBV3_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001045916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic exoenzyme target recognition
                     domain-containing protein"
                     /protein_id="HHU3846984.1"
                     /translation="MNKWLVKASSLVVLGGMVLSAGSRVLADTYVRPIDNGRITTGFN
                     GYPGHCGVDYAVPTGTIIRAVADGTVKFAGAGANFSWMTDLAGNCVMIQHADGMHSGY
                     AHMSRVVARTGEKVKQGDIIGYVGATGMATGPHLHFEFLPANPNFQNGFHGRINPTSL
                     IANVATFSGKTQASAPSIKPLQSAPVQNQSSKLKVYRVDELQKVNGVWLVKNNTLTPT
                     GFDWNDNGIPASEIDEVDANGNLTADQVLQKGGYFIFNPKTLKTVEKPIQGTAGLTWA
                     KTRFANGSSVWLRVDNSQELLYK"
     gene            complement(11530..13080)
                     /gene="purH"
                     /locus_tag="ACUBV3_000451"
     CDS             complement(11530..13080)
                     /gene="purH"
                     /locus_tag="ACUBV3_000451"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000197234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HHU3846985.1"
                     /translation="MTRKRALISVSDKSGIIDFAKELKNLGWDIISTGGTKVALDDAG
                     VETITIDDVTGFPEMMDGRVKTLHPNIHGGLLARRDADSHLQAAKDNNIELIDLVVVN
                     LYPFKETILRPDVTYDLAVENIDIGGPSMLRSAAKNHASVTVVVDPADYATVLGELAD
                     AGQTTFETRQRLAAKVFRHTASYDALIAEYFTAQVGEAKPEKLTITYDLKQAMRYGEN
                     PQQDADFYQKALPTDYSIASAKQLNGKELSFNNIRDADAAIRIIRDFKDSPTVVALKH
                     MNPCGIGQADDIETAWDYAYEADPVSIFGGIVVLNREVDAATAEKMHPIFLEIIIAPS
                     YSEEALAILTNKKKNLRILELPFDAQAASEVEAEYTGVVGGLLVQNQDVVAENPSDWQ
                     VVTDRQPTEQEATALEFSWKAIKYVKSNGIIITNDHMTLGLGAGQTNRVGSVKIAIEQ
                     AKDHLDGAVLASDAFFPFADNIEEIAAAGIKAIIQPGGSVRDQESIDAANKHGLTMIF
                     TGVRHFRH"
     gene            complement(13099..13719)
                     /locus_tag="ACUBV3_000452"
     CDS             complement(13099..13719)
                     /locus_tag="ACUBV3_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000183891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3846986.1"
                     /translation="MTNNNSFNGGHNIGSGNSAGRDQIINITNSQAEQPVAKYDVEPI
                     WRSPITMAVLSWIGFFTSIGSLLPLYNFLKPIISWAKNSFDGNIPNVQGSQIYLFIFL
                     GLFIFSVVIFSLRRITKSQTRYPLGFNYAISGVGKRLNIEKLHITPCPKCGGKLKYYN
                     KPYDWINHYDENGNIKKIKITKRVPALECKRNSEHFWYVDSAGTKI"
     gene            complement(13738..14301)
                     /gene="purN"
                     /locus_tag="ACUBV3_000453"
     CDS             complement(13738..14301)
                     /gene="purN"
                     /locus_tag="ACUBV3_000453"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HHU3846987.1"
                     /translation="MKIAVFASGNGSNFQVIAEQFQVSFVFSDHRDAYVLERAQNLAI
                     PSFAFELKEFENKTAYEQAIVDLLDKHEIDLVCLAGYMKIVGETLLSAYEGRIINIHP
                     AYLPEFPGTHGIEDAWEAGVDQSGVTIHWVDSGVDTGQVIQQVRVPRLADDSLESFET
                     RIHETEYQLYPAVLDSLGVERKEWNTK"
     gene            complement(14469..15491)
                     /gene="purM"
                     /locus_tag="ACUBV3_000454"
     CDS             complement(14469..15491)
                     /gene="purM"
                     /locus_tag="ACUBV3_000454"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HHU3846988.1"
                     /translation="MSEKNAYAQSGVDVEAGYEVVERIKKHVARTERAGVMGALGGFG
                     GMFDLSQTGVKEPVLISGTDGVGTKLMLAIKYDKHDTIGQDCVAMCVNDIIAAGAEPL
                     YFLDYVATGKNEPAKLEQVVAGVAEGCVQASAALIGGETAEMPGMYGEDDYDLAGFAV
                     GVAEKSQIIDGSKVKEGDILLGLASSGIHSNGYSLVRRVFADYTGDEVLPELEGKQLK
                     DVLLEPTRIYVKAALPLIKEELVNGIAHITGGGFIENVPRMFADDLAAEIDEDKVPVL
                     PIFKALEKYGDIKHEEMFEIFNMGVGLMLDVNPENVDRVKELLDEPVYEIGRIIKKAD
                     DSVVIK"
     gene            complement(15519..16973)
                     /gene="purF"
                     /locus_tag="ACUBV3_000455"
     CDS             complement(15519..16973)
                     /gene="purF"
                     /locus_tag="ACUBV3_000455"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HHU3846989.1"
                     /translation="MTYEVKSLNEECGVFGIWGYPQAAQVTYFGLHSLQHRGQEGAGI
                     ISNDNGKLYGYRNVGLLSEVFKNQSELDNLTGNAAIGHVRYATAGSADIRNIQPFLYK
                     FHDGQFALCHNGNLTNAISSRKELEKQGAIFNASSDTEILMHLIRRSHNPSFMGKVKE
                     ALSTVKGGFAYLLMTEDKLIAALDPNAFRPLSIGQMQNGAWVISSETCAFEVVGAKWV
                     RDVEPGEVILIDDSGIQCDRYTDETQLAICSMEYVYFARPDSTIHGVNVHTARKNMGK
                     RLAQEFKQDADIVIGVPNSSLSAAMGFAEESGLPNEMGLVKNQYTQRTFIQPTQELRE
                     QGVRMKLSAVSGVVKGKRVVMIDDSIVRGTTSRRIVGLLREAGATEVHVAIASPELKY
                     PCFYGIDIQTRRELISANHAVDEVCDIIGADSLTYLSIDGLIKSIGLETKAPNGGLCV
                     AYFDGHYPTPLYDYEEEYLRSLEEKTSFYIQKVK"
     gene            complement(17385..21110)
                     /locus_tag="ACUBV3_000456"
     CDS             complement(17385..21110)
                     /locus_tag="ACUBV3_000456"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HHU3846990.1"
                     /translation="MNKRIFVEKKADFGIKSASLVKELTHNLQLASLKDLRIVQVYDV
                     FNLAEDLLARAEKHIFSEQVTDRLLTEAEITAELDKVAFFAIEALPGQFDQRAASSQE
                     ALLLLGSDSQVKVNTAQLYLVNKDIAEAELEAVKNYLLNPVDSRFKDITLPLEVQAFS
                     VSDKTISNLDFFETYQADDFAAYKAEQGLAMEVDDLLFIQDYFKSIGRVPTETELKVL
                     DTYWSDHCRHTTFETELKNIDFSASKFQKQLQATYDKYIAMRDELGRSEKPQTLMDMA
                     TIFGRYERANGRLDDMEVSDEINACSVEIEVDVDGVKEPWLLMFKNETHNHPTEIEPF
                     GGAATCIGGAIRDPLSGRSYVYQAMRISGAGDITTPIAETRAGKLPQQVISKTAAHGY
                     SSYGNQIGLATTYVREYFHPGFVAKRMELGAVVGAAPKENVVREKPEAGDVVVLLGGK
                     TGRDGVGGATGSSKVQTVESVETAGAEVQKGNAIEERKIQRLFRDGNVTRLIKKSNDF
                     GAGGVCVAIGELADGLEIDLDKVPLKYQGLNGTEIAISESQERMSVVVGPSDVDAFIA
                     ACNKENIDAVVVATVTEKPNLVMTWNGETIVDLERCFLDTNGVRVVVDAKVVDKDLTV
                     PEARTTSAETLEADMLKVLSDLNHASQKGLQTIFDSSVGRSTVNHPIGGRYQITPTES
                     SVQKLPVQYGVTTTASVMAQGYNPYIAEWSPYHGAAYAVIEATARLVATGADWSRARF
                     SYQEYFERMDKQAERFGQPVSALLGSIEAQIQFGLPSIGGKDSMSGTFEELTVPPTLV
                     AFGVTTADSRKVLSPEFKAAGENIYYIPGQAISEDIDFDLIKANFSQFEAIQAQHKIT
                     AASAVKYGGVLESLALMTFGNRIGASVEIAELDSSLTAQLGGFVFTSVEEIADVVKIG
                     QTQADFTVTVNGNDLAGASLLSAFEGKLEEVYPTEFEQVDAIEEVPAVVSDVVIKAKE
                     IIEKPVVYIPVFPGTNSEYDSAKAFEQVGASVNLVPFVTLNEAAIAESVDTMVANIAK
                     ANIIFFAGGFSAADEPDGSAKFIVNILLNEKVRAAIDSFIEKGGLIIGICNGFQALVK
                     SGLLPYGNFEEAGETSPTLFYNDANQHVAKMVETRIANTNSPWLAGVEVGDIHAIPVS
                     HGEGKFVVSASEFAELRDNGQIWSQYVDFDGQPSMDSKYNPNGSVNAIEGITSKNGQI
                     IGKMGHSERWEDGLFQNIPGNKDQKLFESAVKYFTGK"
     gene            complement(21233..21937)
                     /gene="purC"
                     /locus_tag="ACUBV3_000457"
     CDS             complement(21233..21937)
                     /gene="purC"
                     /locus_tag="ACUBV3_000457"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HHU3846991.1"
                     /translation="MTNQLIYTGKAKDIYSTKDENVIRTVYKDQATMLNGARKETIDG
                     KGALNNQISSLIFEKLNMAGVVTHYIEQISKNEQLNKKVDIIPLEVVLRNVTAGSFSK
                     RFGVEEGHVLETPIVEFYYKNDNLNDPFINDEHVKFLGIVNDEEIAYLKGETRHINEL
                     LKDWFAQIGLNLIDFKLEFGFDKDGKIILADEFSPDNCRLWDADGNHMDKDVFRRDLG
                     SLTDVYQVVLEKLIAL"
     gene            complement(22061..22300)
                     /locus_tag="ACUBV3_000458"
     CDS             complement(22061..22300)
                     /locus_tag="ACUBV3_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HHU3846992.1"
                     /translation="MSRDEVFEKMLELLRQQLGDPQLDITPESSLHDDLAIDSIALTE
                     FIINLEDVFHLEIPDEAVEHMSSVQQLLDYIIEHK"
     gene            complement(22311..23303)
                     /gene="plsX"
                     /locus_tag="ACUBV3_000459"
     CDS             complement(22311..23303)
                     /gene="plsX"
                     /locus_tag="ACUBV3_000459"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000716823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HHU3846993.1"
                     /translation="MKKIAIDAMGGDYAPKAIVEGVNQAISDFSDIEVQLYGDQKKIE
                     KYLTVTERVSIIHTEEKINSDDEPAKAVRRKKQSSMVLGAKAVKDGVAQAFISAGNTG
                     ALLAAGLFVVGRIKGVDRPGLMSTMPTLDGVGFDMLDLGANAENTASHLHQYAILGSF
                     YAKNVRGIEVPRVGLLNNGTEETKGDSLHKEAYELLAAEPSINFIGNIEARDLMSSVA
                     DVVVTDGFTGNAVLKTMEGTAMSIMGSLKSSIKSGGVKAKLGALLLKDSLYQLKDSMD
                     YSSAGGAVLFGLKAPIVKCHGSSDSKAVYSTLKQVRTMLETQVVDQLVDAFTDE"
     gene            complement(23381..24259)
                     /locus_tag="ACUBV3_000460"
     CDS             complement(23381..24259)
                     /locus_tag="ACUBV3_000460"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HHU3846994.1"
                     /translation="MELIFRIFEMKKTLYITNKLFFANWFVPVIGAVLIQVIGIIVSR
                     RLVVKLLPDDYKFNLYIALLLFVPILASILLFLIYFQKRDFTSLGFTKDNNVLTTISG
                     FITGSLLMFFIYICHWLSGFVEISSSNSFSFGPFFFLFLAYFIQSLTEEILFRGYVMT
                     TVTKFKGSFAGVLCNSMLFSFIHFRNYGITAIALFNLFLLGIIFSILFNMTKNILFVT
                     GVHTTWNFTMGCVLGNKVSGGDSPVSLFRITENSSFALWNGGDFGFEGGVLCSLILVG
                     CLAYFSLESVRVSVRY"
BASE COUNT         7497 a         5167 c         4152 g         7469 t
ORIGIN      
        1 acttatcatt gctgtttctt agaacttagt ttggtccctc gctaacttta tgttatactg
       61 aatatattaa gcgctttctt aaagaggaga ggaccgtgta ttatgactat ttatgaccaa
      121 attgagtctg ctcttgattt aatgactgat ttagaacgtg aaattgcttg ttattttatg
      181 gggcaaccca tttcaaaaga cgctcttgct tctaccattg tcactaagca actacacatt
      241 tcacaagcag cattaacgcg ttttgctaaa aaatgtgggt ttaaaggcta tcgtgaattt
      301 gtttttgaat atttaaaaag ccatgagacc atcagtcaac aattatacgg tcttcaaaat
      361 gacaacacta agaaagtatt tatgaattat caagagatgg tttctaaatc cgctgatatt
      421 attgatgaag aacagcttct agaagtatca catatgattg agcaagctga tcgtgtttat
      481 ttttatggca aagggagctc atcgctagta gctaaagaat ttaaaatacg ccttatgagg
      541 ctaggagtga tttgtgaggc tctggatgat acagatagtt tctcatggac taatagtatt
      601 gttaatgata gatgcctcgt cattgccttc tcactttctg ggaataccaa ctctgttatc
      661 ggtgccttaa aaatcgcaag tagtcacgga gctaaaaccg ttctatttac aaaacaacct
      721 cacacaattg actatgcttt tgacaagatt attcaggtgg cttctgctag gcatttagac
      781 tacggcaata gaatttcgcc tcagatccca atgttaatca tggtagatat tatctatgca
      841 caatttttag atattaataa aatagaaaaa gagcgaattt ttagagaaac cattattcaa
      901 cgctaaatga aaatcgagta gaaataatct actcgatttt ttgctttgta aattgacttc
      961 ctgtcagttg atcaaaaata tagtctttta cttgcactgt cattggatca tgtccataat
     1021 ctggtaataa aagatgcttt ttagttgatg tcagacgatt aaaaatggca aattgagttg
     1081 atggaggaca aatatcatct ttcaaggctg ttagtaaaac aacggggcaa gaaatcctat
     1141 gtgcaaaatt tttaacatca atataagcca aagtttttaa cacactattc tctgttttat
     1201 gaaaaggatc actatattta aaatagcgga agagttcatc atagggctca ctgacacccc
     1261 ctaaatctaa tactcgtcta aagtctgaca aaaatgggta aacagcaacg gtcttgacga
     1321 ttttgggatt aagagctgca gctatcaaag caagtgctcc accttgagac cagccataac
     1381 tataaagttg attactatca acactctcta atgtagcaat gatatcaatc aattggaaaa
     1441 catcaagata aatgtcttta taaaaaagat gatttggtcc tgagatcatt cctcgcacaa
     1501 tttggccctt aactgtaatg ccatcaaaat gccccttgtc ttgagactgt ccagcttgac
     1561 ctcgaacatc cattgaaaca acgccgtaac ctgctgctac ataattaagt tggtcactcc
     1621 agtcaggact ttggttttgg tagccatgaa aataaaaaac tacaggatag ggtttatttg
     1681 ttttagggaa taaacatttg gcatagacaa tagagtcatt gctaccttta aaatgaagat
     1741 cataagcttg tgcaaaagta atattgaaat cttttttgtc tagacgatac tcaatattcc
     1801 cttgatattt cattgtttgt tttttccaga agtcatcaaa atcctcggga atttggtctt
     1861 gacctaggta ttctctcatg tcatctagcg acattgtttc aatcatatac ccgtcacccc
     1921 ctgtttttaa aatggtagta agctcctagc ataccagctt ggttttcgtg ttgtgcaaaa
     1981 actattgctg ttttttcagc aagacttgtc acaagatttc gtttcaaaga ttcactaagt
     2041 ttatcttgta aataatcttt ttgagccatg attccaccac ctaaaacaac tttttcagga
     2101 tttaccacat aaaccatatt agcgattcct tgaccaaggt agtttatcat acggtctata
     2161 cttgcaatac atttctcatt accttcttta gcctcctgaa aaatacgacg tccatcccat
     2221 cggctaattt catctccatg cgctttggca acatcagcaa tcagtgcagt tgtagaggct
     2281 aaatcttgaa aatcaccatc tgaaaggtgc atatatccaa cttcacaagc agaattacta
     2341 aagccatgaa aaactgtttt atcaataatc agacaaccac caatacctgt tccaatagta
     2401 agacaaagcg ctattgaact atctttggca ctacctgaga cagcttcagc taatcctgca
     2461 caattaacat cattttcaat ttctgtgcgt acttgataag tgtcttctaa aactttctta
     2521 aattgtgtcc cagcatagtt tggaatttga ggaccagagt aaaatatgca tccttcatct
     2581 ggatctacca tgccagcaga tgagatagca attccatcaa tgctcccttc tgttagatag
     2641 ttatcgataa tttgacaaac tttttgtaaa atgcctggac cacctttgta ggcttctgtg
     2701 gcaagctcac ttgcctcaac aatacaacct aaattatcca caatgccatg cttaatcatt
     2761 gtaccgccaa tatcaattgc tactgttctg gtcataatta tctacctcac tttaacaaaa
     2821 ctgtttttta gcatgtctaa tcatttctgc tgcctcttta ataataggtt tatcttcttc
     2881 tgttacagat gctaaaggta accgaacgga acctatagtt agttgctcat tgatttccaa
     2941 tacagattta atcacagcat acatattacc atgacctgaa caaagctttg tgataatatc
     3001 gttaattgta aattgaagtt cacgcgcttt ttctaaatct ttatcaacaa ttaactgatt
     3061 taaagtcaag tataattctg gcattacgcc ataggtacca ccaatacctg cagctgctcc
     3121 catgagacga ccacccaaaa attgttcatc tggaccatta aaaacaatat gattttctcc
     3181 tccaatagcc acaaaattct gaatgtcttg aactggcatg gaagaatttt taacaccaat
     3241 tacttgcgga ttttgtaaca tttttcgata taaatccgat gtcaaagcaa cccctgctaa
     3301 ttgaggaata ttgtaaataa taaaatcagt ttgtggtgct gcttgactaa tagtattcca
     3361 ataatctgct atggcatact ctggtaatcg aaaataaatt ggtggaattg ctgcaatagc
     3421 atcaacacct atagcttcag catgcatagc caactctacg ctatccttag tattattaca
     3481 tgccacatgc gctattactg ttagtttacc cttagcaacg gacataacat tttccagtac
     3541 aagtttacga tctgctacac tttggtaaat acattctcct gatgaaccat taacataaag
     3601 tccttgaaca cctttatcaa taaaataatt ggtcagggct tttactcgtt cgggacaaat
     3661 atcaccctta tcatcatagc aagcataaaa tgcaggaata attccttgat atttttgtaa
     3721 atctttcatt gataatctcc ttgttgtttg ctgaaatagg caaattgttt ttgtaaaata
     3781 ataattttaa agtaagcaaa ggcggtataa gcaaaaataa atatagctga cactgtaagc
     3841 ataaataaga gactagactg caccataata aaccaagtca tgattaataa aaacatcaat
     3901 acaccagtcc atttcaagtt taaacatgcc attataaagc tctttttcac ggtatctaaa
     3961 agagacattt cgtagcgggc agccatagga aataggtata tgctcgtcat cacagtcaat
     4021 aacaaaaagg cgtaacaagt tatcttcaag aaagacacaa tgaaacctat ttgataatga
     4081 agaattgtta aatcaaatat gatcacaacc aaaatactta gttcaaccaa acctaaaagc
     4141 ataccttgga accactcttt ttttaggtgt tttatataca atattaaaag atttgtctga
     4201 tcaccttcta atttttgcca caacgttcga tacaaactaa ttttagcagc accaatagtt
     4261 accaatggta ggcaagttat cataaaaaga aggtttagta atgctagatc aaaaacaaca
     4321 ttgcaacctt gcataaaggt gttgttaaca tcaaaaatcg ctgctattaa ttgatttgct
     4381 tttttcacaa tatactccct gtatttctta cgccattaac actttcaata cccctttttt
     4441 gacataatta cctagacttc ccataccatt tggctgatga gcttcttctg ggaaaaagaa
     4501 ggctatataa ttatctctca aatcaaaaga agtctcttct gagcacttga taaatcctat
     4561 atcgcctaag tattcctcta cctctacctt atcacctttt cccaatttga taacttcatg
     4621 tccttcaatg acataatgta tatcaaggta ttttttatgg tattcaaaaa tatgatcaaa
     4681 tgactcacta agttgatttg attggaccat gagtatcact tctgggctaa tatgataggt
     4741 gcctatatct aaattcctta aatcatgact tagtagataa tcaatcgcta aatctaaatt
     4801 aggattaata tctttataat gcgttaaatt caataaatga tcgtaaatca taataaatcc
     4861 tacctatcct ttaacagctc ccattgtaat cccctgagta aaggattttt ggaaaactaa
     4921 gaatactgtc acaataggaa cagcagctaa ggctgcacct gccataatta agccatagtt
     4981 tgttgccatt tcagcctgca tagtagctac cccaagagaa atggtcagat tatttctaga
     5041 agttagcata actaattgca taaaatagtc attccaagta ttgataaatg tgaaaatagc
     5101 tagggcagca aatccaggtt tgacaattgg aaatgctaca ttaataaatg ttcgaatctc
     5161 tccacaacca tcaattttag ctgattccag taattctgtt ggaatatttt cagaaaactg
     5221 tttcatcaag aagaccccaa aaggccaacc aacaagaggt aaaataaccg cccataaggt
     5281 atcatgtatc cccataaaat tgataattct aactaatggc actaaaacaa cttgtttagg
     5341 taaagccata gcagcaataa aaagggagaa caatattttt tgaccgtaaa atcgtttctt
     5401 agctaaaaca tagcctgcca ttgacgatgt acagcaaact aggaacattg tcataattga
     5461 gataaagaca ctattccaaa gccatctcaa agctgggttt tgtacagtta aagctttaaa
     5521 attttctaag gtcggtgctt tgggccacca ttgtgggggg atgataatag tatcaggttg
     5581 agatttaaaa gctcctgtca taatccaata aaatggaaag ataaaaagca ctgtcaaaag
     5641 acaaaggata agagccgtta aaaagttata agcactgaat gttttctttt tcatgttttc
     5701 ctcctaatat tccacatcat ttcctaagat cttaaattga gcaaaactta ttaaagcaat
     5761 cattactgct aggaatactc ccatagtatt agcatagcca tattctgata gtttaaaggc
     5821 tttttcataa agataataca tcaaagtgct tgttgaataa tttggtccac cagatgttag
     5881 gagctggatt aaggcaaaac attgaaatga attaattgtt gttataactg caatatagag
     5941 tgtagttggt aagagactcg gccatttaat ttgccaaaaa acttgcattt cattggctcc
     6001 atcaacacgc gcagcttcgc aaagagaatt atcaatattt cccatcgccg caatatatag
     6061 aataataggt tgaccaactg aagtcgttag caaaataata ataatagcaa gtaatgccca
     6121 gtgcttatca ccaagccagc taatattctg ttcaatgaca tggcctgatt tcaaaatgta
     6181 atttaaaatt ccggacatag ggtcataaat ccatttccaa acaactgtga ctgcaacact
     6241 accagttact actggcaaga agaagacaca acggtagaaa gagcgtgaaa aaacattttt
     6301 ttcataagta ttggcagcaa caaaaagtga gaagaaaacc actactggca cagagccaat
     6361 cacaatgatt actgtattta tcaaggattt catgaaaata gaatcatgaa acatacgatt
     6421 gtaattagct aacccaataa aagtgaactg tgtcatagaa taattaaaaa aactagtgac
     6481 aaagcccata accatcggtg caaaaacaaa aattaagaaa aataagagaa taggcgctag
     6541 aaaagcataa gctatcatcg tttccctcat ctttaactta ttagtacgca tacggtactc
     6601 tcctttacat cttaacttga ctaaaaactc ctcttctatc ccaatatgtg attgaccata
     6661 gaaaaggagt ctccttaatt atttagctgc ttttttaatg gtatcatttg ctttttgagt
     6721 aaagtctttc aaagcatctg ccggtttttc atcaccattg gatacagatt gaaccattgg
     6781 gaaccataag gttctcattt cagaaaatcc atcgatagtg ttgtaatatg gtgaataata
     6841 ttgagtccat tttgaaatct tcatcatacg tttatcacct ttataaagat ccccaaatga
     6901 tgttctaact gggaaagcac ctgtacgtat aacatctttt ggtccccatt ttttatcgtc
     6961 agcaataaaa gtgataaatt tcttagaggc ttttactttg ttttcatctt tattattaaa
     7021 gaccgcaaaa ccattaacaa ggtattctaa atctggtttt ccatcttctg atgggaatgg
     7081 cacttcaagg taatccactt tacttgactc taataatttt gcttgagttt ttggttgagc
     7141 tggagcccat aggatagtga aagcagtttg tccattagca aagttttgaa tgtcagctga
     7201 gccatcatac tgagacccat tcatcaagta gcctttctta atccattcaa ctattttttt
     7261 cattgatttt acagaatttt tagtgtcagt ggtatatttt gttacttctt tatctgttat
     7321 tggagcacta taaagattag caaaaaatgc acgcggtcct tgatctcctc cttgcccgtt
     7381 tgcaaagaat gaacctggtg tatagccttt attttttagt gcttttagta ctttttcaaa
     7441 atcactagta gtccaacctt cttttacaag tttcaaaact cctgcatctt taagcatttt
     7501 tttattgaac gccatataaa atggggcaga acttattgga tacatgtagg ctttatcgcc
     7561 agacttagaa gcttgaatga tgttcttatt attgacatcc ttaataaatt ggtctgtaaa
     7621 taaatcattc aaatctgcta atttaccatt tttaccatat tgaataattc gccctggtgc
     7681 atcaaaaagc acatcaggtg ctgtccctgc ttcaattgct gtagtgattt tttcaggtcc
     7741 agatgtgaaa tcaattgtct ctagttttac ttttatatta ggatttttct tttcaaaagc
     7801 cttgatgact tttttctcat aagtacctac tccatcctta gccttttctt gagtaaaggt
     7861 tggaaaagcc caccacgtaa tttcgacttt atccttctcg gcagccataa cagattgact
     7921 actgtctaca caagcaaaca ttgataatgc tgcagctccg aggcaaaaac caatcacact
     7981 ttttttgata ctcataacta ttcccctttt ttattttgat tttgattttt gagtaacact
     8041 tttaactaag tcctgagatg aaacgctccg ctatttcttt tggtctagtg atagcaccac
     8101 caactacaat tcctgcaaca cctatatgat taatttcatt agcttgctta ggagtatgaa
     8161 ttttaccttc cgcaatcaca tctataccgg cttgacaaag cttattaagg agttctatat
     8221 ccggtccttc ttcttggcgg ctgtaatctg tgtatccaga tagagttgta cccacaaaat
     8281 caactcctgc ttcaaaagca tttttacctt cttcaaaagt acttatatca gccattagca
     8341 actgttcagg atatttccct tttatctttt gaataaactc agctacactc aaaccatcat
     8401 gacgctctct aagtgtacaa tctaaggcta ttactgcaat atctaaacta gctaattgat
     8461 ccacctctgt catcgtagca gtgataaatg gttcttgtgg aggatattca cgtttaataa
     8521 tgccgatgat aggtaaatta gtaacttctt gaatttcctt aatgtcgcgg acactattgg
     8581 ctcttatacc aaccgctcct gcttcttgag ctgccaaagc taaaagaggc ataacacctc
     8641 cactttcagt ataaagaggc tccccaggca aagcttgaca tgacacaata atgccatttt
     8701 ttatttgctt tttaaaagct tctttactta gatgtggcat attctcactt ccttatcttt
     8761 tttaatttta gatatctaca aaccttatta tatctgtttt taaagcgttt tcaataatct
     8821 ttacagactt agattttagt tttaacgttt tgatttagtc acggttaacg tgaaagtagt
     8881 ttcagactta tagcttaatc tcctcaattt aataaaaatt agataagaac cttgaaagtc
     8941 tattcaaggc atataaaaaa gaagccaaat agcttccttt tttatattat ttgttaaatg
     9001 atacgtgaac atggtcataa tggttggcag taacgccacc acgatctggc attgcattcc
     9061 aagtattagc aggtccataa atactatttg tatttgagta aaacttttgt tgccagataa
     9121 catatgaaat gttatttgct gccatatttt gtgtagagta ctgtgcaact tcattaccaa
     9181 gtgcttggtt tttacctaca ataaagtcga ctgctaaacc tttaccatga tcacctggat
     9241 cacctgcacg gtatgtactg aattcattaa ctccataagt tgacgctact ttttctttat
     9301 aagctgcaac atgaggttgg agccctgcat tttcaggatg tgcagctact gcatttgttg
     9361 ttgaagctgg ttgtgctacc ggtgttgctg ttggagcttt ttgtgctacc ggaacgctct
     9421 taacttcagt cgcttgtaac ttactgtctg tagctgttga agtcgtagtc acaggaactg
     9481 ctggagctga tacatgctct ggtgatgcac cagtttctac tttaggagtg actactttaa
     9541 cacttgccac tctaggggct gctacagttc ttaccggtgc tactttagct actggagctg
     9601 gtgtttcagc ggcaacagaa gctggtgata ctgttgttga ctgactgact gacgtctgag
     9661 ttggtttaac ttcctcttta gctgctggaa cttctgaagt aatcgacttc acaggagctg
     9721 ttgatacctg ttcattagct gctgcttgac taacagcttg ctcttgtgct aatacttctt
     9781 ttgatttcaa agctggcgca gaagaatatg tcttcattgg cgaaacaatc gttgttgctg
     9841 cttctggtgt cataccttcc gaaattgtat tgagagaaac tttttggtct gcaacagaaa
     9901 cttgattggt tttcaaatcc acagtagctg ttgtttgacc agcagcattt gttgctggtg
     9961 tttctatttt cattgaagtg gcagtatgac tcttctgatc gtaagttact gtcagtgttg
    10021 tctcaggata aataagattg atatctgcaa tgttattaat ttttgctaag acattcatat
    10081 caattgacat tgcttctgaa ataacgctta gtgtatcacc atatttcaca gtatatgatg
    10141 atttattgtc ttgctttacc aaatcagcct ttacctctga aacagtacgt gctgtccacg
    10201 tcgtatctgt ttcttgtgct tgaacacttg cgactgataa tagcgaagct gccattgtcg
    10261 atgtcaatag taccttttta ttcattttca ttgcttcaat tcctatttcc tttttattag
    10321 tccgagacta taatatcaca ctttactgga ctataaaatt attttcagac ggtttaaaga
    10381 aattgtactt agttagtaac aaaactgtca tttaaaacaa tgaatcaata cctcaaacta
    10441 tttgtaaagc agttcttgac tgttgtcaac gcgaagccaa actgaactac cattagcaaa
    10501 gcgtgtctta gcccaagtta aaccagctgt tccttggatg ggtttttcta cagtcttaag
    10561 agttttagga ttaaagataa agtaaccacc tttttgaaga acctggtcag ctgtcaaatt
    10621 accattagca tcaacctcat caatttctga tgctggtata ccattatcgt tccaatcaaa
    10681 cccagtcggc gttagggtgt tatttttgac taaccaaaca ccattaacct tttgtaattc
    10741 atctactcga tacactttta atttactaga ttgattctgt acaggagctg attgtaatgg
    10801 cttaatgctt ggagctgatg cttgcgtttt tccactaaag gtcgcaacgt tagcaattag
    10861 tgacgttgga ttgatacgtc catggaaacc attttgaaaa ttagggttag ctggtaaaaa
    10921 ttcaaaatga aggtgaggtc ccgtcgccat accagttgct cctacgtaac cgatgatatc
    10981 tccttgtttg actttttccc cagtcctagc caccacacgt gacatatgag cgtaaccact
    11041 atgcattcca tccgcatgtt gaatcatgac acaatttcct gctaagtctg tcatccaaga
    11101 aaagttggct ccagctcctg caaatttcac agtaccatct gccactgccc taataatcgt
    11161 tccagtcgga acagcataat ccaccccaca atgtccagga taaccattga aacctgttgt
    11221 aattctacca ttatcaattg gacggacata agtatccgct aaaactcggg aacccgcaga
    11281 taaaaccata ccacctaaaa caactaagga acttgcctta actaaccatt tattcaagaa
    11341 aaacctccaa atagaattaa tataatgaag tgctcaaaca cttgcaatta acttattcga
    11401 aaatttaaat caaaatatca aaaaaagtac tcaccttgat gagtacttag gaggtttaat
    11461 aaaaagaaaa gtttttagga tatacgtagt atacctgagt taacttaaga aaacctaaac
    11521 tactattttt taatgtctaa aatgtctcac gcctgtgaag atcatggtca agccatgttt
    11581 gtttgcggcg tcaatagatt cttggtcacg aactgaacca cctggttgga tgattgcttt
    11641 gatccctgcg gcagcgattt cttcaatgtt gtccgcaaat gggaagaagg catctgatgc
    11701 tagaacggca ccgtcaaggt ggtccttagc ctgctcgata gcaatcttga ctgagccgac
    11761 acggttggtt tgacctgcac cgagtccaag cgtcatgtga tcgttagtaa taataatccc
    11821 gttagactta acatacttga tagccttcca ggaaaactca agggcagtcg cctcttgttc
    11881 tgttggctgg cggtctgtca ccacttgcca gtcagatgga ttttcagcca caacgtcttg
    11941 gttttgcacc aaaagtccac caactacgcc agtgtactca gcttccactt cgctggcagc
    12001 ttgggcatca aacggcaact caaggatacg caagtttttc tttttatttg tgagaatagc
    12061 tagcgcttct tctgagtatg atggtgcgat gatgatttcc aagaagatag ggtgcatctt
    12121 ctcagctgtc gctgcgtcaa cttcacggtt aaggacaaca attccaccaa agattgaaac
    12181 tgggtcagct tcataagcgt aatcccaagc tgtctcaata tcatcagctt gtccgatacc
    12241 acatgggttc atgtgtttga gggcaacaac cgttggactg tctttgaaat cgcggataat
    12301 acggattgct gcatcagcat cacggatatt attgaaggac aattcttttc cattgagctg
    12361 tttagctgaa gcgattgagt agtctgttgg taaggctttt tggtagaaat cagcgtcttg
    12421 ttgtggattt tctccgtaac gcatagcctg tttaaggtca taagtgatgg tcaatttttc
    12481 aggcttagcc tctcccactt gagctgtgaa gtactcagca atcaaagcgt cataggatgc
    12541 cgtatgacgg aagaccttag ctgccaagcg ttgacgagtt tcaaatgtcg tctgaccagc
    12601 gtcagccaat tctcccaaaa cagtggcata gtccgctggg tcaaccacaa cggttacgct
    12661 agcgtggttt ttagcggctg agcgaagcat tgatggaccg ccgatatcga tgttttccac
    12721 cgccaaatcg taggtcacgt ctgggcgaag gatggtctcc ttgaagggat agaggttgac
    12781 aaccacgagg tcaatcaact caatattgtt gtccttagca gcctgaaggt ggctgtcagc
    12841 gtcgcgacga gccagaagcc caccgtgaat gtttgggtgg agggtcttaa cacgaccgtc
    12901 catcatttct gggaatccag tcacatcgtc gatggtaatg gtctcaacac cagcatcgtc
    12961 aagggcaacc ttagtcccac cagttgagat aatatcccaa cccaagtttt tcaattcttt
    13021 tgcaaagtca ataattcctg acttatcaga aactgagatt aaagcacgtt ttcttgtcat
    13081 tgtttttcct tttatcaatt aaattttcgt tccagctgaa tctacatacc aaaaatgctc
    13141 gctatttctt ttacattcta aagccggcac tctcttggta attttgatct ttttgatatt
    13201 tccattttcg tcataatgat taatccaatc atatggctta ttgtagtatt tcaattttcc
    13261 accacatttt ggacaaggag ttatatgcaa cttttcaata ttcaatcttt tacctacacc
    13321 actgattgca tagttaaaac ccaaaggata gcgagtttga ctcttagtaa ttcttcgcaa
    13381 agaaaaaata acaactgaaa aaataaacaa acctaaaaaa ataaacaaat aaatttgact
    13441 accctggaca ttgggaatat ttccatcaaa agaattttta gcccaactta taatcggttt
    13501 taaaaaatta taaaggggta acaaacttcc tatcgaagtg aagaagccaa tccaactcaa
    13561 cactgccatt gtaatcggac ttctccaaat aggttcaaca tcatatttag cgactggttg
    13621 ctctgcttga gaattagtaa tattaattat ttgatctcta ccagctgaat tgcctgaacc
    13681 aatattatgt cctccattaa aactattatt atttgtcata ttcttttcta ctctccttta
    13741 tttagtattc cattctttcc tctccactcc caagctatct aaaacagctg gatagagttg
    13801 atattcagtt tcgtggatac gggtttcaaa gctttctagg ctatcatctg ctaggcgtgg
    13861 cacgcgcact tgttggatga cctgaccggt atccacacca gagtccaccc aatggatggt
    13921 cacgccagac tggtcaacac cagcttccca agcatcctcg ataccgtggg tacctggaaa
    13981 ttcaggcagg taggctgggt gaatattgat gatacggccc tcataggctg agagtaaggt
    14041 ttctccaaca atcttcatat agcccgccag acagaccaaa tcaatctcgt gtttgtccaa
    14101 caaatcaaca atggcttgct cgtaagccgt cttattctca aactccttga gttcaaaagc
    14161 gaaacttgga atggctaagt tctgagcacg ctctaaaaca taggcatcac gatgatctga
    14221 aaagacaaaa ctaacttgaa actgctctgc tatgacctga aagttggaac cattaccaga
    14281 agcaaaaaca gcgattttca tattcgatac aaagggatga ttcggcttgc cgaaaaattt
    14341 cgtagaaaaa taggaaaacg aacaacgacg cttgcgtcta agtcgtttta tcttttttcc
    14401 tagaaattta atcccgtgtt cagttaggca aactaactaa acactccttt cttataaaat
    14461 ttagtgactt atttaatcac cacactatcg tctgctttct tgatgatacg accgatttca
    14521 tagactggtt cgtccaaaag ttctttgaca cggtcaacat tttcagggtt aacatccagc
    14581 ataagaccga cacccatgtt gaagatttca aacatttctt cgtgcttgat gtcaccatat
    14641 ttttcaagcg ccttgaaaat cggaagtaca ggcaccttat cctcatcgat ttccgcagcc
    14701 aaatcatccg caaacatacg aggaacattc tcgataaaac caccacccgt gatgtgggcg
    14761 ataccgttaa ctagttcttc cttgatcaat ggcagagctg ctttaacata gatacgagtt
    14821 ggctcaagaa ggacatcctt gagttgtttg ccttcaagct ctggaagcac ctcatcacca
    14881 gtgtagtcag caaagacacg acgtaccaat gaataaccat ttgaatggat accacttgaa
    14941 gcaagtccaa gaagaatatc cccttctttt acctttgaac cgtcgatgat ttgagatttt
    15001 tcagccacac caacagcaaa gcctgcaagg tcataatcat cttcgccata cataccaggc
    15061 atctcagccg tttcaccacc gataagagct gcgctagctt gaacacaacc ttcagcaaca
    15121 ccagcgacaa cctgttccaa tttggcaggt tcgtttttac cagtcgcgac atagtcaagg
    15181 aagtaaaggg gctcagcacc tgctgcaata atatcgttga cacacatggc aacacagtct
    15241 tgaccgattg tgtcgtgctt gtcgtacttg atagcaagca tgagtttagt tccgacaccg
    15301 tcagtccctg aaatcaagac aggttcttta acacctgttt gtgacaggtc aaacatacca
    15361 ccaaagccac cgagagctcc catgacacca gcacgttctg tgcgagcaac gtgtttcttg
    15421 atacgttcga caacttcgta gcccgcttct acatcaacac cagactgggc ataagcattt
    15481 ttttcagaca tgttttctcc ttatataatt agcagagctt atttcacttt ttgaatataa
    15541 aaacttgttt tttcttctaa acttctgagg tattcttctt cataatcata aagcggtgtt
    15601 ggataatgtc catcaaagta agctacgcat aaaccaccat ttggtgcttt tgtttcgagt
    15661 ccaattgatt taattaaccc atcgattgat agataagtta gactatctgc gccaataata
    15721 tcacacactt cgtcaacagc gtgatttgct gaaattaatt ctcgtcttgt ttgaatatca
    15781 attccataaa aacaaggata ctttaattct ggactagcta tagcaacatg tacttcagta
    15841 gctcctgctt ctcttaataa tcctacaatc cttctagaag tcgttcctct tacaattgag
    15901 tcatcaatca taacaacgcg ctttcctttg acaacaccag ataccgctga taatttcatt
    15961 cgaacacctt gttcccttaa ttcttgtgtc ggttgaataa aggttcgctg cgtatactga
    16021 ttttttacaa ggcccatctc atttggtaat ccggattctt cagcaaagcc catagcagcc
    16081 gataaggacg aatttgggac accaattaca atatcagcat cctgtttaaa ttcttgtgca
    16141 agacgctttc ccatattttt tcgagccgta tgaacattaa caccatgtat agttgaatcc
    16201 ggccttgcaa aatagacata ttccatcgaa caaattgcca gttgcgtttc atcagtataa
    16261 cgatcacatt gaataccact atcgtctata agaataactt caccaggttc aacatctcta
    16321 acccattttg cgcctaccac ctcaaaagca caggtctcac tggaaataac ccaggcacca
    16381 ttttgcattt gtccaattga caaaggacga aaggcattag ggtcaagagc agcaattaat
    16441 ttatcttctg tcatcagtag ataggcgaaa cctcccttta cagtgcttaa agcttctttt
    16501 accttcccca taaaacttgg gttatggctt cgacgaatca aatgcattaa aatttcagta
    16561 tctgaggagg cattgaaaat tgcaccttgc ttttctaatt ctttccttga ggaaatagca
    16621 tttgtcaaat taccattatg acataaagca aattgcccat catgaaattt ataaagaaaa
    16681 ggctgaatat tgcgaatatc tgcagaacct gcagtagcat accgaacatg tccaatagcc
    16741 gcattcccag ttaaattatc taattcagat tgattcttaa aaacttcaga aaggagccca
    16801 acatttcgat aaccatagag tttcccatta tcatttgaaa taataccagc tccttcttga
    16861 ccgcgatgtt gaaggctatg aagcccaaag taagtgactt gagctgcctg aggatacccc
    16921 cagataccaa agactccaca ttcttcattt agagatttta cttcgtatgt cattcttttt
    16981 tcctaattct ttttgtatct cttcttaagt agctttgatg attttctcaa ttttggatct
    17041 gtcgtcatca atgccaacat taaaatagaa aatctatcaa gctagccatt gaaccttagt
    17101 ctcaatgact cccaaatacc aatacggcgg aaagatacgg ttaagctcgc ttcgttcaaa
    17161 agatagcgaa tttctatcta acctcgcctt cactaattca aggcgagggc taaagctagg
    17221 acaaaagtcc taagcctcca tattttttat aatctagttc gcaaaacgta ggggttgagt
    17281 gggctctaat tcgctgataa atcaactctt acagccccac tcaactgtgc ggagggggac
    17341 gacaaaatcg aattcctttg gaattaccga tttttgtccc actctcactt ccctgtaaag
    17401 tattttacag cactttcaaa gagtttttgg tctttgtttc cagggatatt ttggaagagt
    17461 ccgtcttccc agcgttctga gtgccccatc ttaccgatga tttgaccatt cttgctggta
    17521 atcccttcga tggcattgac agagccgttt ggattgtatt tagaatccat agatggttgt
    17581 ccgtcaaagt ccacatattg gctccagatt tgaccattgt ctcttagctc tgcaaattca
    17641 gaagcgctga caacaaattt accttcaccg tgtgaaactg gaatggcatg aatatcgccg
    17701 acctcaactc ctgccaacca aggtgaattg gtatttgcga tacgagtctc aaccatcttt
    17761 gcaacgtgct ggttagcatc gttatagaag agggttggac ttgtctcacc agcttcctcg
    17821 aagtttccgt atggaagaag acctgattta acaagggctt ggaaaccatt acagataccg
    17881 atgataaggc cacctttttc gatgaagctg tcaatagctg cgcgaacctt ctcgttaagc
    17941 aagatattga cgataaactt agcagagcca tctggttcat ccgctgctga gaaacctcca
    18001 gcaaagaaga tgatatttgc cttagcaata ttagcaacca tcgtatcaac tgactcagca
    18061 atagcagcct cattcaaggt tacaaatggc accaagttga cgctagctcc aacctgttca
    18121 aaggccttag ctgaatcgta ctctgagtta gtaccaggaa agactgggat gtaaactact
    18181 ggtttttcaa taatttcctt agccttgata acaacatctg ataccacagc aggaacttcc
    18241 tcaatagcat ctacctgttc aaactctgtt gggtaaactt cttctaattt accttcgaag
    18301 gctgatagaa ggcttgcacc agctaggtca tttccattga cagtgactgt aaagtctgcc
    18361 tgagtttgac cgatcttcac aacgtcagca atttcctcaa ctgatgtaaa gacaaagcct
    18421 ccgagttgag ctgtcaagct gctgtcaagc tctgcaattt caacagaagc accgatacgg
    18481 ttaccaaaag tcatgagagc aagactttct aggacaccac cgtatttaac ggctgaggca
    18541 gcggtaatct tatgttgagc ttgaatagcc tcaaactggc taaagttagc cttgataagg
    18601 tcaaaatcaa tatcttctga aatagcttga cctggaatgt agtagatatt ctcaccagcc
    18661 gctttaaact caggagagag aaccttgcgg ctgtccgctg ttgtcacacc gaaagctacc
    18721 aaggttggtg gtactgtcaa ttcttcgaaa gtaccagaca tagagtcctt accgccgatt
    18781 gatggcaaac caaattgaat ctgagcctca atagaaccaa gaagagctga tactggctga
    18841 ccaaaacgct ctgcctgttt atccatacgc tcgaaatact cttggtaaga gaaacgtgca
    18901 cgagaccagt cagcacccgt tgctaccaag cgagctgtcg cttcaataac agcataggca
    18961 gcaccgtgat aaggtgacca ctctgcaata taaggattgt aaccttgagc cataacagat
    19021 gctgttgttg tcacaccata ttgaactggc aatttttgaa cagaactttc tgtcggtgtg
    19081 atttggtaac gaccaccgat tgggtggtta acggttgaac gaccaacaga tgagtcaaag
    19141 atagtttgaa gacctttttg actagcgtga ttgaggtcag acaagacctt aagcatatct
    19201 gcttcaagtg tctctgctga tgttgtgcgc gcttctggaa ctgttaagtc cttgtcaacg
    19261 actttggcat caactacgac acgaacaccg ttggtatcaa ggaaacagcg ttccaaatca
    19321 acaattgttt caccattcca agtcatgaca agatttggtt tttcagtaac tgtcgcaaca
    19381 acaactgcat cgatattttc cttgttacag gctgcgatga aggcatccac atcacttgga
    19441 ccaacaacaa ctgacatcct ttcttgagat tctgagatag caatttcagt accattaaga
    19501 ccttggtatt taagtggcac cttgtccaaa tcgatttcaa gaccatcagc taattcaccg
    19561 atggcaacac agacaccacc tgcaccgaag tcatttgatt tcttgattag gcgagtgaca
    19621 ttaccatcac ggaaaaggcg ttgaatctta cgttcttcga tggcattccc tttttgtacc
    19681 tctgcgccag ctgtttccac agattcaacc gtttgaacct tagatgaacc tgtcgcaccg
    19741 ccgacaccgt cacgacctgt tttaccaccg agcaagacaa ccacatcgcc tgcctctggt
    19801 ttttcacgaa ccacattttc cttaggtgca gcaccaacca cagctccaag ctccatgcgt
    19861 ttagctacga agccagggtg gaagtactca cgcacataag ttgtcgcaag cccaatttgg
    19921 ttaccatatg aagaatagcc gtgcgccgca gtcttagaaa taacctgttg tggcaattta
    19981 ccagcacgtg tttccgcaat cggagtcgtg atatctcctg cgcctgaaat acgcatagcc
    20041 tgataaacgt atgaacgtcc tgacaatggg tcacgaatgg caccaccgat acaagtcgcc
    20101 gctccaccaa atggctcaat ttctgttggg tggttgtgag tctcgttctt aaacatgagg
    20161 agccaaggct ctttcacacc atcaacatct acttcaattt caactgagca ggcattgatt
    20221 tcatctgaga cttccatatc gtccagacga ccattggcac gctcataacg accaaagata
    20281 gtcgccatat ccataagtgt ctgcggcttt tcagaacgac caagctcatc acgcatggca
    20341 atgtatttgt cataagtcgc ctgcaattgt ttttggaatt tagaggccga aaaatcaatg
    20401 tttttcaatt cagtttcaaa ggttgtgtga cggcagtggt ctgaccaata cgtatccaaa
    20461 actttcaact cagtctcagt tggcacacgc ccgattgatt tgaaataatc ttggatgaag
    20521 agaaggtcat cgacctccat agccaatccc tgctctgcct tataggctgc aaaatcgtca
    20581 gcctgataag tttcgaagaa atcaagatta gagattgtct tatcagacac agagaaagcc
    20641 tgcacttcaa gtggcaaggt aatatccttg aaacgtgaat caacgggatt caaaagatag
    20701 ttcttaacgg cttcaagctc tgcttctgca atatccttat tgaccaagta aagctgagct
    20761 gtattgacct tgacctgact gtcacttcca agcaatagca aagcttcttg cgaactagca
    20821 gcacgttggt caaattgacc aggaagcgcc tcaatggcaa agaaggcaac cttatcaagc
    20881 tccgcagtga tttcagcttc tgtcaaaaga cggtctgtca cctgctcaga gaaaatatgc
    20941 ttctcagcac gcgccagcaa atcctcagcc aaattgaaga catcatagac ctgcacgata
    21001 cgcaaatcct tcaaagaagc cagttgtaga ttatgcgtca actctttcac aagactagcc
    21061 gatttaatgc caaagtcagc ctttttctca acaaaaatac gtttattcat ataagatacc
    21121 aagtccgaca agggagcgac tcgaaaatag gaggacgtct gaaattgaga tttcagtaag
    21181 cccaatcttt tttctgagtt tcccgaacgg attcaattcc ttcctcttct aattacaatg
    21241 ctatcaactt ctcaagtaca acttgataga catcggtcaa gctaccaaga tccctacgaa
    21301 aaacatcctt gtccatatga ttaccatcag catcccagag acggcagttg tcaggtgaga
    21361 actcatcagc caagatgatt ttgccatcct tgtcaaatcc gaactccaac ttgaagtcaa
    21421 tcaagttaag cccaatctga gcaaaccaat ctttcaagag ttcattaata tggcgagtct
    21481 ctcctttcag ataagcaatc tcctcatcat taacaatacc caaaaacttc acatgctcat
    21541 cattgataaa aggatcattc agattatcat ttttgtagta aaattccacg attggcgttt
    21601 caagaacgtg tccttcttcc actccaaaac gttttgaaaa tgagcctgct gtcacattcc
    21661 gtagcaccac ctcaagtgga ataatatcaa cttttttatt caattgttcg ttttttgaaa
    21721 tttgctcaat ataatgtgtc accacaccag ccatatttaa tttctcaaaa ataagagatg
    21781 aaatctgatt atttagtgct cccttgccat caatagtctc cttacgagct ccattcaaca
    21841 tagtagcttg gtctttatag accgttctaa tcacattttc atcttttgta ctataaatat
    21901 ctttcgcttt tcctgtataa ataagctggt tagtcacttg attattcgcc tttctcttat
    21961 tttaagagtt attctaacac acaacattat ctaatacaac aaaaacttta tttaaaatca
    22021 aaaaaaagaa gaaacgttcg tattttcttc ctttttatta ttacttatgt tcaatgatat
    22081 aatctaatag ttgttggacg cttgacatgt gttcgacagc ctcatctgga atctctagat
    22141 gaaacacatc ttccaaattg ataataaact ctgttaaagc gatagagtca attgctaaat
    22201 catcatgtaa cgatgactct ggtgtgatat ctaactgtgg gtcaccaagt tgctgcctta
    22261 ataattccag catcttttca aaaacttcat ctcttgacat gagttcactc ctattcatct
    22321 gtaaaggcat caactaattg atcaactact tgagtttcca acattgtacg aacttgcttc
    22381 aaagtagaat aaacagcttt agaatcactt gatccatggc attttactat tggagctttt
    22441 aaaccaaaaa gaaccgctcc acccgcgctt gaatagtcca tactatcttt tagctgatat
    22501 aggctatctt ttaatagaag tgctcctaat ttagctttca cgccacctga tttaattgaa
    22561 gattttaggc ttcccataat actcatagct gtaccttcca ttgtctttag aacagcattt
    22621 ccagtaaaac catcagtgac aacaacatct gctacactag acatcaaatc acgtgcttca
    22681 atatttccaa taaaattaat agaaggctct gctgctaaca gttcataagc ttctttatga
    22741 agagaatcgc cttttgtttc ttccgttccg ttatttaaga gacctacacg aggaacttca
    22801 ataccacgga cattttttgc ataaaacgac cctaaaatag catattgatg aagatgagaa
    22861 gctgtgtttt ctgcattagc tcctaaatca agcatatcaa aacccacacc atctaacgta
    22921 ggcatagttg acattaagcc tggtcgatca acacctttta tacgtcctac tacaaacaaa
    22981 ccagcagcta ataaagctcc tgtattccct gcagaaataa aggcctgtgc aacaccatct
    23041 ttgactgcct tagctcctaa gaccatcgaa gactgttttt tacgacgaac agctttagca
    23101 ggctcatcat cagaattaat tttttcttca gtatgaataa tactaactcg ctccgttact
    23161 gttaaatatt tctcaatttt tttctgatcg ccatagagtt gtacttcaat atcactaaaa
    23221 tctgaaatag cttgattaac accctctaca atagctttgg gagcgtaatc tccacccata
    23281 gcatctatag caattttctt catgaatgaa ctcctcataa aaacttagtg gctatattat
    23341 agcacgtttc tttatttttt tcacaagaag gcaataatag ctaatatcta acagatactc
    23401 tcacgctttc caaagaaaag tatgctaaac aacctactaa tatcaaagaa catagtactc
    23461 ccccttcaaa accaaaatcc ccaccattcc aaagagcaaa gctagaattt tcagtaattc
    23521 taaaaagaga aacaggagaa tcccccccac taaccttatt acctaataca catcccatag
    23581 taaaattcca tgtagtatga actcctgtaa caaaaagaat atttttggtc atattaaaaa
    23641 gaatagaaaa aataatacca agtaaaaata aattaaaaag tgctattgct gtgataccat
    23701 agtttctaaa gtggataaaa gaaaacaaca tagaattaca taaaacacct gcaaaactac
    23761 ctttaaattt ggtaactgtt gtcataacat atcctctaaa taaaatttcc tctgtcaagc
    23821 tttggataaa gtatgcaaga aataaaaaga aaaaaggtcc aaaagaaaag gaattgctgg
    23881 aagaaatttc cacaaaacct gataaccagt ggcaaatata aataaaaaac atcagtaaac
    23941 taccagtaat aaatcctgag atcgttgtta aaacattatt atctttagta aatcccaagg
    24001 aagtaaaatc ccttttttga aaatagatta aaaatagtaa aatacttgcc aaaataggaa
    24061 caaatagtaa aagcgcaatg tataaattga atttataatc atcaggtagc aattttacta
    24121 caaggcgtct acttacaatg atgccgataa cttgaattag aactgcacca ataactggca
    24181 caaaccagtt agcaaaaaag agtttattgg taatgtataa tgtttttttc atctcaaata
    24241 tcctaaaaat taattccata ttagtttatc ctaatattaa aaaaa
//
LOCUS       DBJYSH010000014        23102 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.014, whole genome shotgun sequence.
ACCESSION   DBJYSH010000014 DBJYSH010000000
VERSION     DBJYSH010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 23102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23102
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.014"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            complement(28..480)
                     /locus_tag="ACUBV3_000461"
     CDS             complement(28..480)
                     /locus_tag="ACUBV3_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0050896 - response to stimulus [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HHU3846995.1"
                     /translation="MTQKYERILIAIDGSYESELAVEKGINVALRNDAELLLTHVIDA
                     HAYQSEGVFSDYVFDRQEQESADVLAYFEKLAHSKGLTKIKKITEIGNPKTLLAKDIP
                     IREKADLIMVGATGLNTFERLLIGSTSEYILRHSKVDMLVVRDSKKTL"
     gene            complement(498..1700)
                     /locus_tag="ACUBV3_000462"
     CDS             complement(498..1700)
                     /locus_tag="ACUBV3_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HHU3846996.1"
                     /translation="MEDKLFNKHFIGITILNFIVYMVYYLFTVIIAFIATKELGVSTS
                     QAGLATGIYIVGTLIARLIFGKQLEVLGRKLVLRGGAIFYLLTTLAYFYMPSIGVMYL
                     VRFLNGFGYGVVSTATNTIVTAYIPADKRGEGINFYGLSTSLAAAIGPFVGTFMLDNL
                     HINFKMVIVLCSILIAIVVLGAFVFPVKNITLNPEQLAKSKSWTIDSFIEKKAIFITI
                     IAFLMGISYASVLGFQKLYTTEINLMTVGAYFFIVYALVITLTRPSMGRLMDAKGDKW
                     VLYPSYLFLTLGLALLGSAMGSVTYLLSGALIGFGYGTFMSCGQAASIKGVEEHRFNT
                     AMSTYMIGLDLGLGAGPYILGLVKDGFLGAGVQSFRELFWIAAIIPVVCGILYFLKSS
                     RQVETKTI"
     gene            1856..2515
                     /locus_tag="ACUBV3_000463"
     CDS             1856..2515
                     /locus_tag="ACUBV3_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HHU3846997.1"
                     /translation="MNHQILQNYINSHNLPIIEKDYHKYLTFESLEEDYTYILKDGIV
                     KQSVLSKYGTEFNLRYVTGLEITSILNTDYSQHMGEPYNVRIESETAHFYKVRRSTFL
                     KDINNDIELQGYVKDFYHNRLEKSMKKMQCMLTNGRIGAISTQLYDLSKMFGEERDNG
                     DIYINFVITNEELGKFCGISTGSSVSRILKQLKDDHIIRIEKQHIIITNVEKLKDHIV
                     F"
     gene            2539..4824
                     /locus_tag="ACUBV3_000464"
     CDS             2539..4824
                     /locus_tag="ACUBV3_000464"
                     /EC_number="3.4.14.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001270186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidyl-peptidase"
                     /protein_id="HHU3846998.1"
                     /translation="MRYNQFSYIPTKPNEAFEELKGLGFPLNKKNSDKANLEAFLRHS
                     FLNQTDTDYALSLLIVDAKTDALTFFKSNSDLTLENLQWIYLQLLGFVPFVDFKDPKA
                     FLQDINFPVSYDNIFQSLHHLLACRGKSGNTLIDQLVADGLLHADNHYHFFNGKSLAT
                     FNTNQLIREVVYVEISLDTMSSGEHDLVKVNIIRPTTEHTIPTMMTASPYHQGINDPA
                     ADQKTYQMEGALAVKQPKHIQVDTKPFKEEVKHPSKLPISPATESFTHIDSYSLNDYF
                     LSRGFANIYVSGVGTAGSTGFMTSGDYQQIQSFKAVIDWLNGKVTAFTSHKRDKQVKA
                     DWSNGLVATTGKSYLGTMSTGLATTGVEGLKVIIAEAAISTWYDYYRENGLVCSPGGY
                     PGEDLDVLTELTYSRNLLAGDYIKNNDCYQALLNEQSKAIDRQSGDYNQYWHDRNYLT
                     HVNNVKSRVVYTHGLQDWNVKPRHVYKVFNALPQTIKKHLFLHQGQHVYMHNWQSIDF
                     RESMNALLSQELLGIDNHFQLEEVIWQDNTTEQTWQVLDAFGGNHQEQIGLGDSKKLI
                     DNHYDKEAFDTYCKDFNVFKNDLFKGNNKTNQITINLPLKKNYLLNGQCKLHLRVKTS
                     DKKAILSAQILDYGPKKRFKDTPTIKFLNSLDNGKNFAREALRELPFTKDHYRVISKG
                     VLNLQNRTDLLTIEAIEPEQWFDIEFSLQPSIYQLSKGDNLRIILYTTDFEHTIRDNA
                     SYSITVDLSQSYLTIPTNQGN"
     gene            4828..5187
                     /locus_tag="ACUBV3_000465"
     CDS             4828..5187
                     /locus_tag="ACUBV3_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3846999.1"
                     /translation="MKLLTKERFDDSQHFWYQINLLQESNFGAVFDHDNKNIPQVVAT
                     IVDDLQGSGSSNHFWYFGNTTDTSILMIAHLNRKFYIQVNLKDFDFALNLIAINNWKS
                     LLQTQLEALNDTLAIFQ"
     gene            5233..6213
                     /locus_tag="ACUBV3_000466"
     CDS             5233..6213
                     /locus_tag="ACUBV3_000466"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HHU3847000.1"
                     /translation="MSSYWNNYPELKKNIDETNQLIQERIQVRNKDIEAALSQLTAAG
                     GKQLRPAFFYLFSQLGNKENQDTQQLKKIAASLEILHVATLIHDDVIDDSPLRRGNMT
                     IQSKFGKDIAVYTGDLLFTVFFDLILESMADTPFMRINAKSMRKILMGELDQMHLRYN
                     QQQGIHHYLRAISGKTAELFKLASKEGAYFGGAEKEVVRLAGHIGFNIGMTFQILDDI
                     LDYTADKKTFNKPVLEDLTQGVYSLPLLLAIEENPDIFKPILDKKTDMATEDMEKIAY
                     LVVSHRGVDKARHLARKFTEKAISDINKLPQNSAKKQLLQLTNYLLKRKI"
     gene            complement(6298..8046)
                     /gene="cydC"
                     /locus_tag="ACUBV3_000467"
     CDS             complement(6298..8046)
                     /gene="cydC"
                     /locus_tag="ACUBV3_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000472828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0055051 - ATP-binding cassette (ABC)
                     transporter complex, integrated substrate binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033230 - ABC-type cysteine transporter
                     activity [Evidence IEA];
                     GO_function: GO:0050456 - L-cystine reductase (NADH)
                     activity [Evidence IEA];
                     GO_process: GO:0033228 - cysteine export across plasma
                     membrane [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductant ABC exporter subunit CydC"
                     /protein_id="HHU3847001.1"
                     /translation="MFKIPLFKELKTDQWVKPFFKQYKVSLVIALFLGFMTFFSASAL
                     MFNSGYLISKSASLPSNILLVYVPIVLTRAFGIGRPVFRYIERLTSHNWVLRMTSQLR
                     LKLYHSLESNAIFMKRDFRLGDVMGLLAEDINYLQNLYLRTIFPTIIAWLLYSFIIIA
                     TGFFSLWFALMMLLYLAIMIFLFPLWSILANGARQTREKELKNHLYTDLTDNVLGISD
                     WIFSQRGQEYVALHERSESELMAVQKKIRSFNNRRALIVELVFGFLAILVIIWASNQF
                     IGHRGGEANWIAAFVLTVFPLSEAFAGLSAAAQETNKYSDSIHRLNELSETYFETTQN
                     QLPNKPYDFSVKNLSFQYKPQEKWVLHHLDLDIKEGEKIAILGRSGSGKSTLASLLRG
                     DLKASQGKITLGGADVSIVGDCISNYIGVIQQAPYLFNTTLLNNIRIGNQDASEEDVW
                     KVLERVGLKEMVTDLSDGLYTMVDEAGLRFSGGERHRIALARILLKDVPIVILDEPTV
                     GLDPITEQALLRVFMKELEGKTLVWITHHLKGIEHADRILFIENGQLELEGSPQELSQ
                     SSQRYRQLKAADDGDL"
     gene            complement(8039..9757)
                     /gene="cydD"
                     /locus_tag="ACUBV3_000468"
     CDS             complement(8039..9757)
                     /gene="cydD"
                     /locus_tag="ACUBV3_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0055051 - ATP-binding cassette (ABC)
                     transporter complex, integrated substrate binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033230 - ABC-type cysteine transporter
                     activity [Evidence IEA];
                     GO_function: GO:0050456 - L-cystine reductase (NADH)
                     activity [Evidence IEA];
                     GO_process: GO:0033228 - cysteine export across plasma
                     membrane [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductant ABC exporter subunit CydD"
                     /protein_id="HHU3847002.1"
                     /translation="MLDKAVMRLSGIHKLLGLLAGLDVLQAIFIIGQAYYLSLSITGL
                     WEGQKLSSQTVYILLFMVSYLGRHVIDYIKNRKLDDFSTAQSSLLRRQLLDKLFDLGP
                     KVVQEQGTGNVVTMALDGVSLVENYLRLVLNKMINMSIIPWIILAYIFYLDIESGAIL
                     LIVFPLIIIFMIILGYAAQAKADKQYESYQVLSNHFLDSLRGIDTLKYFGLSKRYGKS
                     IYQTSESFRKATMSTLKIGILSTFALDFFTTLSIAIVAVFLGLRLLNEQIYLLPALTI
                     LILSPEYFLPVRDFSSDYHATLDGKNAFQAIQKVLNKTGIKGEQLVIDDWSKESHLDL
                     ENIAIAYDQKRVVEDVTLRFRGHQKVALVGVSGSGKSSLINLLSGFLGPDKGSLKVDG
                     REVVNLDQEDWHKQMIYIPQTPYVFEMSLRDNITFYTPNASDEEVIRAIHMVGLDSLL
                     SELPDGLETRIGNGARPLSGGQAQRIALARAFLDQNRRIMVFDEPTAHLDIETELELK
                     EKMLPLMSDRLVIFATHRLHWLNQMDVIVVMEKGRVVEVGSYQELLAKKGYLYQLKHA
                     MGGNNV"
     gene            complement(9757..10776)
                     /gene="cydB"
                     /locus_tag="ACUBV3_000469"
     CDS             complement(9757..10776)
                     /gene="cydB"
                     /locus_tag="ACUBV3_000469"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001275291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HHU3847003.1"
                     /translation="MSALQFFWFFLIGLLFSGFFFLEGFDFGVGMAVQTLTHNEHEKD
                     QVVETIGPVWDGNEVWLLTGGGAMFASFPYWYASLFSGYYLILLTILFGLIIRGVSFE
                     FRHKVPAEKKQFWNWTLTIGSAIVPFFFGIMFISLIQGMPLDASGNLSAQFSDYFNIF
                     SLVGGVAMVLLAYLHGLNYIALKTEGPIRERARNYAQLLYWVLYLGLALFAVLLYFKT
                     DFFSNHPIVTTIMVLVIVVLAVLAHASTFKGAEMTAFLASGLSLVSVVVLLFQGLFPR
                     VMISSISPKYDLLIQNASSTPYTLKVMSIVAITLVPFVLAYTAWAYYIFRKRITLPVI
                     VTGEK"
     gene            complement(10777..12204)
                     /locus_tag="ACUBV3_000470"
     CDS             complement(10777..12204)
                     /locus_tag="ACUBV3_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HHU3847004.1"
                     /translation="MTIETLARFQFAMTTVFHFFFVPFTIGTCLVVAIMETMYVITKN
                     EEYKKLTKFWGNIMLLSFAVGVVTGIIQEFQFGMNWSDYSRFVGDIFGAPLAIEALLA
                     FFMESTFLGLWMFTWDNKKISKKLHVTFIWLVVFGSLMSAMWILTANSFMQHPVGYEV
                     VNGRAQMTDFLALVKNPQFFYEFTHVIFGAITMGGTVVAGMSAFRLLKSEQLKDTTVE
                     LYKKSVRIGLVVALLGSISVMGVGDLQMKALIHDQPMKFAAMEGDYEDSGDPAAWSVV
                     AWANEAEHKQVFGIKIPYMLSILSYGKPSGSVKGMDTANKELVAKYGKDNYYPMVNLL
                     FYGFRTMAAMGTAIMGVSVLGLFLTRKKKPILYKHKWMLWIVALTTFAPFLANTFGWI
                     VTEQGRYPWTVYGLFKIKDSVSPNVSVASLFVSNTVYFLLFGGLAVMMISLTIRELKK
                     GPEYEDEHGHHGAYTSIDPFEKGAY"
     gene            complement(12307..13515)
                     /locus_tag="ACUBV3_000471"
     CDS             complement(12307..13515)
                     /locus_tag="ACUBV3_000471"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003954 - NADH dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006116 - obsolete NADH oxidation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HHU3847005.1"
                     /translation="MKEILVLGAGYAGLKAVRNLQKQSGDFHITLVDMNDYHYEATEL
                     HEVAAGSQPKEKITFPIKDVINTNKVNFMQDEVLKVDAENKTVTVKNNGELHYDYVVV
                     ALGFVSETFGIKGAMENALQMTNISQAENIHNHIVNTMKLYRETKDENLLKLLVCGAG
                     FTGIELAGAMVDERPKYAALAGVKPEQIEIICVEAATRILPMFDDELAQYGVNLIKDL
                     GINLMLGSMIKEIKPGEVVYGTSKEDEELKSITAGTIIWTTGVSGSPVMGESGFDQRR
                     GRVMVNSDLRDPKYDNVYVIGDVSAFMDTESGRPFPTTAQIATRMGAHVAKNLLHQIK
                     GEATEDFSYSPQGTVASVGNTHGLGVVGKTKIKKYPASVMKKIIMNKSLVDMGGLKEL
                     LAKGRFDLYH"
     gene            complement(13528..14427)
                     /locus_tag="ACUBV3_000472"
     CDS             complement(13528..14427)
                     /locus_tag="ACUBV3_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HHU3847006.1"
                     /translation="MTLPIFLELVEMKAKTASVLPFLIGLCFSAYYYNSINPVYVGLF
                     FVAMFLFNMFVDIWNNYNDYRNAVDLDYKNDTNIIGRENLSLRQIEVIMASLVITSSV
                     IGLVLVSQVGLPLLWMGLFCFGIGVLYSFGPRSLSSLPLGEVFSGLTMGFMISLICVY
                     LNTYQNFSWDILNLSKIFLISLPNTLWIANLMLANNLCDKEEDEKNHRYTLVHYTGIR
                     GGLLLFAISNSIALLAIVFEFLFGLAPVTVLLSLLLIPFIYKQTKLLWQKQVKRETFV
                     CAVRILALGSATQVLTYLIGIIF"
     gene            complement(14835..15134)
                     /locus_tag="ACUBV3_000473"
                     /pseudo
     CDS             complement(14835..15134)
                     /locus_tag="ACUBV3_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037493.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(15321..15758)
                     /locus_tag="ACUBV3_000474"
                     /pseudo
     CDS             complement(15321..15758)
                     /locus_tag="ACUBV3_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037493.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(16363..16518)
                     /locus_tag="ACUBV3_000475"
     CDS             complement(16363..16518)
                     /locus_tag="ACUBV3_000475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3847007.1"
                     /translation="MLVLLSVYTKDNGIGYQEELYMHKRLKWLVAATCLGFILEGRHL
                     ISKKWVK"
     gene            16641..16940
                     /locus_tag="ACUBV3_000476"
     CDS             16641..16940
                     /locus_tag="ACUBV3_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HHU3847008.1"
                     /translation="MMNMQNMMRQAQKLQKQMEQKQADLAASQFTGKSAQELVTVTFT
                     GDKKLISIDYKEAVVDPEDIETLQDMTTQAINDALSQVDDATKKIMGAFAGKMPF"
     gene            17101..18291
                     /locus_tag="ACUBV3_000477"
     CDS             17101..18291
                     /locus_tag="ACUBV3_000477"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HHU3847009.1"
                     /translation="MVMDSLIIKQLIKSTFDIPLQVTYPNGNIETYNGNNPHVKLKLN
                     KNFSVSELSKDPSIVLGEAVMDGDIEIDGSIQELILSAYRCGDSFLRNSKFSKLIPKQ
                     FHDKKHSKSDIQKHYDIGNDFYKLWLDDTMTYSCAYFKHENDSLEQAQLNKVHHILNK
                     LNAQPGGKLLDIGCGWGTLIITAAKEYGLNATGITLSEEQASFITKRIKEEGLENKVT
                     VLIKDYRDIHETYDYITSVGMFEHVGKENLSQYFQTISKRLNINGLALIHGITGQVGD
                     NHGSGTNSWINKYIFPGGYIPRLTENLNHIASAGLQIADLEPLRRHYQKTLELWTKNF
                     HNALPEVQKTHDKRFINMWDLYLQSCAASFESGNIDIFQYLLSKGVSKDTMPMTRDYM
                     YSAN"
     gene            complement(18361..19086)
                     /locus_tag="ACUBV3_000478"
     CDS             complement(18361..19086)
                     /locus_tag="ACUBV3_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001292015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HHU3847010.1"
                     /translation="MSEKYSTGDLAKEAGVTVRTVQYYDKRGILSPSELSEGGRRVYS
                     IADLEKLRQIIYLRDLDFSIDNIKNLFTEDNASQILELFLQVQIRELRLAIDSKKDKL
                     DKAVNLLKTVEKQDSKTLGYLSDIVLMEENKRKWGKLQKSIVLQVVMIIMVYITLIIA
                     AVSLNQRWLMWIAVTVFLIAMNGMVWYFKQQVVYLCPNCHQTFTPTYQAFNLAKHTPK
                     TRKVFCPHCHQKSHCIELAREER"
     gene            complement(19213..19800)
                     /locus_tag="ACUBV3_000479"
     CDS             complement(19213..19800)
                     /locus_tag="ACUBV3_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HHU3847011.1"
                     /translation="MKQLQEYIAFDLEFNTVGEHSHIIQVSAVKYSNHQEIALFDTYV
                     HTKVPLQSFINGLTGITARDIIGAPKIETILTDLQSFVGDTPLIGYNGHKSDLPLLVE
                     NGLDLTSQYQVDLYDEAFVRRSTDLNGIVNLKLTTVADFLGIKGKAHNSLEDARMTAR
                     VYEKFLDLDENKIYLKQQKEVAVDSPFAALGNLFD"
     gene            complement(19844..20380)
                     /locus_tag="ACUBV3_000480"
     CDS             complement(19844..20380)
                     /locus_tag="ACUBV3_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017766787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HHU3847012.1"
                     /translation="MTVEKTVSELAEILGVSRQAMNNRVKTLPEECVEKNSKGVTVVN
                     RDGLIKLEEIYKKTILEEEPIDEEASRRELLEILVDEKNTEITRLYEQLKAKDIQIAS
                     KDEQLHVKDIQIAEKDKQLDQQQQLTLTAMEDTQRLQLELNEAKAEVEEIQEAKEEKI
                     QELEAVKKSFFGRFFNKK"
     gene            20515..21687
                     /locus_tag="ACUBV3_000481"
     CDS             20515..21687
                     /locus_tag="ACUBV3_000481"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HHU3847013.1"
                     /translation="MKHYDTIIIGGGPAGMMAAISSNFYGNKTLLIEKNKRLGKKLAG
                     TGGGRCNVTNNGNLDELLAGIPGNGRFLYSVFSQFDNHDIINFFQDNGVTLKEEDHGR
                     MFPTTDKSRTIINALENKIKELGGQIMTDTEVVSVKKIGDSFYIKTKDTQFASDKLIV
                     TTGGKSYPSTGSTGFGHDIARHFKLEVTDMEAAESPLLTDFPHKKLQGISLDDVTLSF
                     EKHIITHDLLFTHFGLSGPAALRISSFVKGGETIYLDVLPNISVKELETHFQNEREKS
                     LKNALKILLPERLAEFYAEDLPEKVKQVSVKDLETLIQKLKKLPILVTGKMSLAKSFV
                     TKGGVDLKEINPKTLESKKVAGLHFAGEVLDINAHTGGFNITSALCTGWVAGSLHY"
     gene            complement(21731..22513)
                     /locus_tag="ACUBV3_000482"
     CDS             complement(21731..22513)
                     /locus_tag="ACUBV3_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017766788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4931 domain-containing protein"
                     /protein_id="HHU3847014.1"
                     /translation="MCYNNSMNERYIRFNPNIAKQKPSNKDKCPFCDKSQLGKILDVK
                     DDMIWVENKYPTLEETYQTLVIESNDHNGDISVYSESKMRQLLDYLLSKWQLMEESGH
                     YRSVVLYRNFGPLSGGSLRHPHSQIVGLKNVDAYETLNLDWFDGIEVCSETSQQAEIS
                     IANRPMLDFTEFTIKVSKLENAILIADSLPILCHYILNEFLGGRCKSYNLFFYKHDGG
                     LICKIVPRFIVSPYQIGYAIHQVNLPDELEKIKEALRAKYYL"
     gene            22672..22941
                     /gene="rpsN"
                     /locus_tag="ACUBV3_000483"
     CDS             22672..22941
                     /gene="rpsN"
                     /locus_tag="ACUBV3_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001085658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HHU3847015.1"
                     /translation="MAKKSKIAKFQKQQKLVEQYAELRRELKEKGDYEALRKLPKDSN
                     PNRLKNRDLIDGRPHAYMRKFGMSRINFRNLAYKGQIPGIKKASW"
BASE COUNT         7822 a         4430 c         3640 g         7210 t
ORIGIN      
        1 actacctagt actctatttt atttagttta taaggttttc ttactatcac gaacgactag
       61 catatcaact ttcgaatgac gtaaaatgta ttctgaagtt gagccgatca atagtctttc
      121 aaaagtgttt aatcctgttg cgcctaccat aattaaatct gctttttccc ttatggggat
      181 atcctttgcc aaaagagtct taggatttcc gatttcagta atttttttga tctttgtaag
      241 gccttttgag tgtgccaatt tctcaaaata ggctagcaca tctgctgact cttgttcttg
      301 cctatcaaaa acatagtcac taaagactcc ctcactttgg taagcgtgag catctataac
      361 atgcgttagc agtaattcag catcattacg aagagcaaca tttatccctt tttctaccgc
      421 caattctgac tcgtaagacc catcgatagc aataagaata cgctcatatt tttgtgtcat
      481 agcttaattc ctccctttta tatagtttta gtttcaactt gtcgagatga ttttaagaaa
      541 tatagaatac cacaaacaac aggaataatc gctgctatcc agaataattc cctaaaggat
      601 tgcacaccag ctccaagaaa accatcttta acaagtccca aaatgtaagg tccagcacct
      661 aaccctaaat caagacctat catgtaagtt gacatggctg tattgaaacg atgttcctca
      721 acacctttga ttgatgctgc ttggccacaa gacataaagg tgccataacc aaaaccaatc
      781 aaagcacctg atagaaggta ggtaacactt cccatagcac tccctaataa agcaagtccc
      841 aaagttaaga acagataact tggataaagc acccacttat ctcccttagc gtccattaat
      901 cttcccatag atggtctggt taaagtgatg acaagtgcat aaacaataaa gaaataagct
      961 cctactgtca tcaaattaat ttctgttgta tataattttt ggaaacctaa cacggaagca
     1021 taggagatac ccatcaaaaa tgcaataatt gtgataaaga ttgctttttt ctcaatgaaa
     1081 ctatcaatag tccatgattt tgatttagct aactgttctg gatttaaagt aatatttttg
     1141 actgggaaaa caaatgctcc caacactaca atcgcaatta aaatactaca taatacaata
     1201 accattttaa agttaatatg aaggttgtct agcataaatg ttcctacaaa aggaccaata
     1261 gctgcggcta aacttgttga tagaccgtaa aaattaatcc cttcacctct tttatcagct
     1321 ggtatatagg ctgttacaat agtattagtt gctgttgaca cgacaccata accaaaacca
     1381 tttaggaaac gaactaaata cattactccg atacttggca tataaaaata agctaaagtt
     1441 gttagtaagt aaaaaatagc ccctccacgt aaaactaact tacgtcctag aacttctaat
     1501 tgcttaccaa atataagacg agcaatcaaa gtccctacaa tataaatccc cgttgctaat
     1561 cccgcttgac tagtgctaac acctaactct ttagtcgcaa taaaagctat gataacggta
     1621 aacaaataat agaccatata aacaataaag tttaaaatag taatgcctat aaaatgtttg
     1681 ttgaataatt tgtcttccaa tgcaaatggc tccttttttc aatttcattt tcataaacat
     1741 tttacaaaaa tattagaaga tacgatattt caatggtata atataagtgc tagtatcaga
     1801 ctgcattaac aaatgttaag gtggtctatc aaacctaaaa catttggagg aagtcatgaa
     1861 tcatcaaatt ttacaaaatt acattaatag tcataatctt ccaattatcg aaaaagacta
     1921 ccataaatac cttacatttg aaagtttaga agaagactat acttatatcc taaaagatgg
     1981 tattgttaaa caaagtgttc tctctaaata tggtactgag ttcaatctgc gttatgtgac
     2041 aggcttagaa atcacttcta tcctaaatac agactactcc caacacatgg gagaacctta
     2101 caacgttcga atcgaatccg aaactgccca cttttacaaa gtacgtcgct ctaccttcct
     2161 aaaagatatc aataatgata ttgaactgca aggatatgtg aaagatttct atcataaccg
     2221 ccttgaaaaa tctatgaaaa aaatgcaatg tatgctcact aatggtcgaa ttggtgctat
     2281 ctccacacaa ttatatgacc tcagtaagat gtttggtgaa gaacgagata acggtgatat
     2341 ctatattaat tttgttatca cgaacgaaga acttggtaaa ttttgcggta tatccacagg
     2401 aagtagcgtg agccgcattt tgaaacaact aaaagacgat catattattc gtattgaaaa
     2461 acaacatatc attattacaa atgttgagaa attaaaagat catattgtat tttaatataa
     2521 caatagaaag gatatattat gcgttataat caattcagct acattccaac taaacctaac
     2581 gaagcttttg aagagctcaa aggactaggt tttccattaa acaaaaagaa ttctgataaa
     2641 gctaatttgg aagcttttct cagacattct tttttaaatc aaactgatac tgattacgct
     2701 cttagtctcc ttatcgttga tgcaaaaacc gatgctctaa ccttttttaa atcaaatagt
     2761 gacttaacac tagaaaattt acaatggatt tatttacagt tattaggctt tgtccccttt
     2821 gtagacttta aagaccctaa agctttctta caagatatta acttcccagt ctcatatgac
     2881 aatatttttc aaagtctaca tcacttactc gcctgtcgtg gaaaatctgg caatacatta
     2941 atcgaccaat tagttgctga tggtttactt catgcagata atcactacca ttttttcaat
     3001 gggaagtctc tggccacttt caatactaac caattgattc gcgaagttgt ctatgttgaa
     3061 atatccttag atactatgtc tagtggtgaa catgatttag taaaagttaa cattatcaga
     3121 cccactaccg agcatactat ccccacgatg atgacagcta gcccctatca tcaaggtatc
     3181 aatgatcctg ccgcagacca aaaaacatac caaatggagg gtgcgctagc agttaaacag
     3241 cctaaacaca tacaagttga cacaaaacca tttaaagaag aagtaaaaca tccttcaaaa
     3301 ttacccatca gccctgcaac tgaaagcttc acacacattg acagttatag tctcaatgac
     3361 tattttcttt ctcgtggttt tgctaatata tacgtttcag gtgtgggtac tgctggctct
     3421 acgggtttca tgaccagtgg ggattaccaa caaatacaaa gctttaaagc agtcattgat
     3481 tggttaaatg gtaaggttac tgcattcaca agtcataaac gagataaaca agtcaaggct
     3541 gattggtcaa acggccttgt agcaaccaca ggtaaatctt atctcggtac catgtcaact
     3601 ggtttagcaa caactggcgt tgaggggctg aaagtcatta tcgctgaagc cgcaatctcc
     3661 acatggtatg attattatcg agaaaatggg cttgtgtgta gtccaggcgg ctaccccggt
     3721 gaagatttag acgttttaac agaattaaca tactcacgaa acctcttagc tggtgattac
     3781 atcaaaaaca acgattgcta tcaagcattg ttaaatgaac aatcaaaagc aattgaccgt
     3841 caaagtgggg attacaacca atactggcat gaccgtaatt acctaactca cgtcaataat
     3901 gtcaaaagtc gagtagttta cactcatgga ctacaggatt ggaatgttaa gccaagacat
     3961 gtctacaaag ttttcaatgc attgcctcaa accatcaaaa aacacctttt tttacatcaa
     4021 ggtcaacatg tgtatatgca taattggcag tcgattgatt ttcgtgaaag catgaatgcc
     4081 ttactaagcc aagaactact tggcattgac aatcatttcc aattagaaga ggtcatttgg
     4141 caagataata ctactgagca aacttggcaa gttttagatg ctttcggagg aaaccatcaa
     4201 gagcaaattg gtttaggtga tagtaaaaaa cttattgata accattatga caaagaagcc
     4261 tttgatactt attgtaaaga cttcaatgtg ttcaaaaatg atcttttcaa gggaaataat
     4321 aaaaccaatc aaatcactat taatcttcct ctaaagaaaa attatctcct gaatggacag
     4381 tgcaaactcc atctacgtgt taaaactagt gacaaaaagg ccattttatc agcccaaatc
     4441 ttagactatg gtcctaaaaa acgattcaaa gatacaccaa ccatcaaatt cttaaacagc
     4501 cttgataatg gtaaaaattt tgccagagaa gctttacgtg aactcccgtt tactaaagat
     4561 cattatcgtg tcatcagtaa aggtgtcttg aaccttcaaa atcgtacaga cttacttaca
     4621 attgaggcta tcgagccaga acaatggttt gatatcgagt ttagcctcca accaagtata
     4681 tatcaattga gtaaaggtga taatctaagg attatccttt atacaactga ttttgaacat
     4741 accattcgag ataatgctag ttactctata acagtagatt tgagtcaatc ttatttaact
     4801 atcccaacta atcaaggaaa ttaacttatg aaacttctta ctaaagaacg gtttgatgat
     4861 tctcaacact tttggtacca gatcaattta ttacaagaga gtaacttcgg agcagttttt
     4921 gaccatgata ataaaaacat tccacaggtt gttgcaacta ttgttgatga tttacaaggt
     4981 tccggaagtt cgaatcattt ctggtatttt ggcaatacta ctgatacttc catccttatg
     5041 attgctcatt taaatcgaaa attctatatt caggttaatt taaaggactt tgactttgca
     5101 ctcaatttaa tagctataaa taattggaag agtctcctcc aaactcaact tgaagctcta
     5161 aacgataccc tagcaatatt tcaataaata aggtagaatg gagtgacaaa gcaacgcgag
     5221 ggagactgat taatgtcatc ttattggaat aactatcctg aacttaaaaa aaatattgat
     5281 gaaaccaatc aactaattca agaaagaata caggtcagaa ataaagatat tgaagcggcg
     5341 ctaagccaac tcacagctgc gggaggaaaa cagctcagac cagcattctt ttaccttttt
     5401 tctcaacttg gtaataagga gaatcaagat actcagcaac taaagaaaat cgctgcttct
     5461 ttagaaatcc ttcacgttgc tacattaatc catgatgatg tcattgatga ctcaccacta
     5521 agacgtggaa atatgaccat tcaaagcaag tttggcaaag acatcgcagt ttatactggg
     5581 gatttacttt tcacagtctt tttcgatctt attttagaat ctatggctga tacaccattt
     5641 atgaggatta atgcaaaatc tatgcgtaaa attctcatgg gagaattgga ccagatgcac
     5701 cttcgttaca atcaacaaca aggtatccat cactatttac gtgcgatttc aggtaagaca
     5761 gccgaactct ttaaattagc tagcaaagaa ggagcttact ttggtggtgc agagaaggag
     5821 gttgttcgtc tagcaggcca tatcggcttt aacattggta tgacattcca aattttggat
     5881 gatatcctgg attatactgc agataaaaaa acatttaata agcctgtctt agaggattta
     5941 acacaaggcg tttacagcct tcctctactt cttgccattg aagaaaatcc tgatattttc
     6001 aaacctattt tagataaaaa aacagatatg gctactgaag acatggaaaa aattgcttat
     6061 ctcgtcgttt cccatagagg tgttgacaaa gctcgccatc tagctcgtaa atttactgag
     6121 aaagctatta gtgacataaa taagctaccc cagaactctg caaaaaaaca gttgctacaa
     6181 ttaactaatt accttttaaa acgcaaaatt taaataataa aaaaacattc cacaatgcta
     6241 gaaaagcagt tagggaatgt ttttttatta tcatttattt atcgcaccta tcaatcatca
     6301 tagatcacca tcatcagcgg ctttcagctg acggtaacgt tgactacttt gagacaattc
     6361 ttgaggagaa ccttccaact ctaattgccc attttctata aataagatac gatcagcatg
     6421 ttcaatacct tttaagtgat gtgtaatcca aactaaggtc ttaccttcca attctttcat
     6481 aaataccctt agtaaggctt gttcagtaat aggatcaagt ccaacagttg gctcatctaa
     6541 gataacaatt gggacatctt ttagtaagat tctagccaaa gcaattctat gcctttcgcc
     6601 acctgaaaac ctaagtccag cttcatcaac cattgtatag agaccatctg ataaatcagt
     6661 gaccatctct ttcaatccaa ctcgttcaag aactttccat acatcttctt cactagcatc
     6721 ttggtttcca atgcgaatgt tatttagcag ggttgtatta aaaaggtagg gcgcttgttg
     6781 tatcactcca atatagttag aaatgcaatc accaactatt gaaacatcag caccgcctag
     6841 ggtaatcttc ccttgacttg ctttcaagtc gccacgaagt agactagcta aggtactctt
     6901 gccagaacca ctccgcccta aaatagcaat tttttctcct tctttaatat ccaaatctaa
     6961 atgatgcaaa acccatttct cttgtggctt atactggaaa cttaaattct tgacggaaaa
     7021 atcatatggc ttattaggca attgattttg agttgtttca aaatacgttt ctgataattc
     7081 attaagacga tgaattgaat cagaatattt atttgtttct tgagcagcag cagacaaacc
     7141 agcaaatgcc tctgacaagg gaaatactgt tagaacaaat gctgcaatcc agttagcttc
     7201 gccacctctg tgaccaataa actgattact agcccaaata ataaccaaaa tagctagaaa
     7261 gccaaaaact aattccacaa taagagctct acgattatta aaggaacgaa tttttttctg
     7321 aacagccatt agttcacttt ccgaacgttc atgcaaggcg acgtactctt gaccacgttg
     7381 actaaaaatc cagtctgaaa tacctaagac attatccgtc aaatcagtat aaagatgatt
     7441 ttttaattct ttttcacgag tctgacgagc accattagct aaaatcgacc aaagtggaaa
     7501 aagaaaaatc ataatagcta aataaagcaa catcattaaa gcaaaccata gagaaaagaa
     7561 gcctgttgca ataataataa agctatataa taaccacgct ataatcgttg gaaatattgt
     7621 cctaagatac aaattttgca aataattaat atcctccgct aaaagcccca taacatctcc
     7681 taaacgaaaa tcacgcttca taaagatagc attgctttct aaggaatggt aaagcttcaa
     7741 gcgcagttgt gaagtcattc gtaataccca attgtgactc gttaggcgtt ctatataacg
     7801 aaaaacagga cgtccaatac caaaagcacg tgttagtaca attggaacat atactagaag
     7861 aatattactc ggtagtgaag ctgacttact aatcagatag cccgaattaa acataagagc
     7921 actggcagaa aagaaagtca taaaaccaag gaaaagagca attactaagg aaactttata
     7981 ttgtttgaaa aatggtttta cccattgatc tgtctttaat tctttaaaca aagggatttt
     8041 aaacattatt tcctcccata gcatgcttca gttgatagag atatcctttt ttagctaata
     8101 attcctgata agagcctact tctacaactc tccccttctc cataacgact ataacgtcca
     8161 tttggttcaa ccagtgaaga cgatgagttg caaagataac taaacgatct gacatcagtg
     8221 gcaacatttt ctcttttaat tctaactctg tttcaatatc taaatgagca gttggttcat
     8281 caaaaaccat aatacgacga ttttgatcaa gaaaagcacg agccagagct atacgctggg
     8341 cttgtcctcc actaagtgga cgcgctccat taccaatacg cgtttctaaa ccatcgggta
     8401 actcgcttag taagctatct aaacctacca tatgaatcgc tcttatcacc tcttcgtcac
     8461 tagcattggg agtatagaag gtaatattat cacgtaatga catttcaaaa acataaggag
     8521 tttgaggaat atagatcatc tgtttatgcc aatcttcttg atcaaggtta accacttctc
     8581 taccatccac ttttaaagaa cctttatcag gccctaaaaa accactcaga aggttaatta
     8641 agctagactt acctgaacca ctaactccta ctaaagcaac cttttgatgt cctctaaatc
     8701 gtaaggttac atcctcaaca acacgttttt ggtcgtacgc aatagcaatg ttttctaaat
     8761 ccaaatgact ctctttagac caatcatcta tgactaattg ctcaccttta ataccagttt
     8821 tgtttagaac cttttgaatc gcttgaaatg catttttccc gtctaacgtt gcatgataat
     8881 cactagaaaa atctcttacg ggtaaaaagt attcagggga taaaatcaaa attgttaaag
     8941 ccggtaaaag gtaaatctgc tcatttaata atcgtaaacc cagaaaaaca gcaacgatag
     9001 caatggataa agttgtaaaa aagtctaaag caaacgttga taaaatacca attttcaaag
     9061 tactcattgt tgctttacga aaagattcac ttgtttgata aatgctttta ccataacgtt
     9121 tactaagacc aaaatatttg agagtatcaa tgcctcgtaa tgaatctaaa aaatgattag
     9181 aaagcacttg ataggattca tattgtttat ctgctttagc ttgagccgca tagcctaaaa
     9241 taatcataaa gatgataatt aaaggaaaaa cgataagtaa aattgcacct gattcaatat
     9301 ctaaatagaa aatataagct agaataatcc atgggataat agacatgtta atcatcttat
     9361 tcaagactaa acgtaaataa ttttcaacta aagaaacacc atctagtgcc attgtaacaa
     9421 cattaccagt tccttgttct tgaacgactt taggacctag atcaaataac ttatctaata
     9481 attgacgtct caaaagacta gattgtgctg ttgaaaagtc atcaagcttt ctatttttga
     9541 tgtaatcaat aacatgacgg cctaaatatg agaccataaa taacaaaata taaaccgttt
     9601 gagaagaaag tttttgccct tcccacaaac cagttataga aagactcaaa taataagctt
     9661 gtccgataat aaagattgct tgaagaacat caagtcccgc aagcaatcct aacaacttat
     9721 gaatacctga caagcgcata accgctttat ctagcattat ttttctcctg tcacaatcac
     9781 tggcaaggta atacgttttc taaagatata atatgcccat gctgtataag ctagaacaaa
     9841 gggaactaga gtaattgcga caatcgacat tacttttaaa gtatatggcg ttgaggatgc
     9901 attttgaatc agcaaatcat atttagggct aattgatgaa atcataactc gtgggaatag
     9961 accttggaac aacagaacaa ccactgaaac aagactgaga ccactcgcta aaaatgctgt
    10021 catctctgct cctttaaaag ttgaagcatg cgctaataca gctaatacaa caataaccaa
    10081 aaccataatt gtcgtaacta tcggatgatt gctaaagaaa tctgtcttga aatacaagag
    10141 aactgcaaac aaggctaaac caagatagag tacccagtaa agcaactgag cataattacg
    10201 ggcacgctct ctaatcggcc cttctgtctt caatgcaatg tagtttaaac catgaagata
    10261 ggctagcaat accattgcaa ctccacccac caacgagaat atattaaagt aatcactgaa
    10321 ttgagctgat aagttaccac tggcatcaag tggcatacct tgaatcaaac ttataaacat
    10381 aatcccaaag aagaatggaa caatagcgga accaattgtt aaggtccagt tccaaaactg
    10441 ctttttctct gcaggaactt tatgtctaaa ttcgaaggaa acaccacgaa taattaatcc
    10501 aaagagaatc gtaaggagaa tcaaatagta accactaaat agtgaagcat accagtatgg
    10561 aaaagaagca aacattgctc caccaccggt aagaagccaa acttcattac catcccaaac
    10621 tggaccaatg gtttctacta cttgatcttt ttcatgctca ttatgtgtta aagtttgaac
    10681 agccatccca accccaaagt cgaatccttc aaggaagaag aatcctgaaa ataggaggcc
    10741 gattaggaaa aaccaaaaga attgtaaagc actcatttag tatgctcctt tctcaaaggg
    10801 atcaattgaa gtatatgcac catgatgtcc atgttcatct tcatactctg gccctttctt
    10861 caattctctt attgttaggc ttatcatcat caccgctaaa ccaccaaaca ataagaagta
    10921 cacagtattg gaaacaaata atgatgcaac tgaaacgttt ggagagacag agtccttaat
    10981 cttgaaaaga ccatagacag tccaagggta tcgtccttgt tctgtcacaa tccaaccaaa
    11041 tgtattagct aagaatggtg caaaggttgt taaagcaaca atccatagca tccatttgtg
    11101 tttataaaga attggttttt tcttacgagt taaaaagagt cctaaaacag aaacacccat
    11161 aatagctgtt cccatcgccg ccattgtacg gaaaccatag aaaagtaggt taaccattgg
    11221 atagtagtta tctttaccat acttagcaac caattcttta ttagctgtat ccataccttt
    11281 cacagaacca gatggtttcc cgtaagataa aatactcaac atataaggaa tcttgatccc
    11341 aaaaacttgt ttgtgttctg cttcatttgc ccaagcaact acactccaag ctgcaggatc
    11401 acccgaatct tcataatcac cttccatagc agcaaatttc attggttgat cgtgaatcaa
    11461 ggctttcatt tgtaaatccc caactcccat aactgaaatt gaaccaagaa gtgctactac
    11521 taaaccaata cgaactgatt tcttataaag ttcaaccgtt gtatctttca gctgttctga
    11581 ttttaagagg cggaaggctg acataccagc aaccactgta ccacccatcg taatggcacc
    11641 aaaaataaca tgggtaaatt catagaagaa ctgtggattt tttacaagtg caagaaaatc
    11701 agtcatttgc gctctaccgt ttacaacttc ataaccaaca ggatgttgca tgaagctatt
    11761 agcagtaaga atccacatcg ctgacattaa tgagccaaaa accactagcc aaatgaatgt
    11821 cacatgtaat tttttactaa tttttttgtt atcccatgta aacatccata aaccaaggaa
    11881 agtagattcc ataaagaaag ctaataaagc ttcaatagcc aatggagctc caaaaatatc
    11941 acctacgaaa cgtgaatagt ctgaccagtt cattccaaat tggaactctt gaatgatacc
    12001 tgtcacaaca ccaactgcaa aactaaggag catgatatta ccccaaaatt ttgtcaattt
    12061 tttatactct tcattttttg tgataacata cattgtctcc ataatagcaa ccactaggca
    12121 tgtaccaatt gtaaatggta caaagaaaaa gtggaaaact gtagtcatcg caaattgaaa
    12181 acgagctaaa gtttcaattg tcatacaaaa cctcttattt tgttttaatc atttctttta
    12241 tagaaatatc tgcaagagct agtttcctag cttcgtaaca attggtaaat aacttacttt
    12301 aatatcttaa tgatataaat caaaacgtcc cttagctaga agttctttaa gtccacccat
    12361 atcaacaagt gatttgttca tgataatctt cttcataacc gacgcagggt attttttaat
    12421 tttagttttt ccaacaacac caagtccatg cgtatttcct actgaagcaa ctgtgccttg
    12481 cggactatac gaaaagtctt ctgtagcctc acctttgatt tgatgtaaaa ggtttttagc
    12541 aacatgagct cccatacgag ttgcaatttg agctgttgtt gggaatgggc gaccagactc
    12601 agtgtccata aatgcagaca catcaccgat aacataaaca ttatcatatt ttggatcacg
    12661 caaatcactg ttgaccatta cacgtccacg acgttgatca aatcctgatt ctcccataac
    12721 cggacttcca ctaacacctg ttgtccaaat gatagtacct gcggtaatag atttgagttc
    12781 ttcatcctct ttactagtac cataaacaac ttcacccggt ttgatctcct taatcatact
    12841 tccaagcatc aaattaatac ctaaatcctt aattaagttg acaccatatt gcgctaattc
    12901 atcatcaaac attggtagga tgcgagttgc tgcctctaca caaataattt caatttgttc
    12961 aggttttaca cccgctaaag cagcatattt tggtcgctca tcaaccatag ctccggctaa
    13021 ctcaatacct gtaaaaccag caccacatac taagagtttg agaagatttt catcttttgt
    13081 ctcacgatac aacttcattg tattaacaat gtgattatgg atgttttctg cttgcgaaat
    13141 attggtcatc tgcaaagcat tctccatagc acccttgatg ccaaaagttt cagatacaaa
    13201 gcctaaggca actacgacat aatcataatg gagttcaccg ttatttttaa ctgttactgt
    13261 tttattttca gcatcgactt taagcacctc atcttgcatg aagttaacct tattagtgtt
    13321 gataacatcc ttaattggga atgtaatttt ttcttttggc tgagagcctg cagctacttc
    13381 atgtaattct gtagcttcgt agtgataatc attcatgtca acaagggtaa tatgaaaatc
    13441 acctgattgt ttttgtaaat tacgaactgc tttcaaaccg gcataaccag cacctaaaac
    13501 taggatttct ttcatgataa aactcctcta aaaaatgatt ccaattagat acgttaagac
    13561 ttgggttgca gatcccagtg ctagaatacg taccgcacaa acaaatgttt ctctcttaac
    13621 ctgtttttgc cacaatagtt ttgtctgctt ataaataaat ggtattaata acaaactcaa
    13681 gagtactgta acaggcgcaa gtccaaaaag aaattcaaaa acaattgcca ataaagctat
    13741 actattagaa atagcaaaca gcaagagtcc acctcttatc cctgtataat gtacaagagt
    13801 atagcgatga tttttttcat cttcttcttt gtcgcacaga ttattcgcca acattaggtt
    13861 ggcaatccat aaggtgttcg gcaaggaaat caagaaaatt ttgcttaagt tgaggatatc
    13921 ccacgaaaag ttttgataag tgtttagata tacacaaatt aaactaatca taaatcccat
    13981 tgtcaagcca gaaaatactt ctcctaaggg cagactagat aaagatcttg gaccaaagga
    14041 atataaaaca ccaattccaa aacaaaaaag tcccatccat aaaagaggca gaccgacttg
    14101 ggaaactaaa actaaaccaa tcacactaga agtaatgact aaacttgcca taataacttc
    14161 tatttggcgt agggataggt tctcacgacc aataatgtta gtgtcattct tataatccaa
    14221 atccacagca ttccgataat cattgtaatt attccaaatg tcaacgaaca tattgaataa
    14281 aaacatagca acgaagaata aaccgacata tacagggttt attgaattgt agtaataagc
    14341 actaaaacac aaaccaatca aaaatggtaa aacactagct gttttggctt tcatttctac
    14401 caactctaga aagattggta atgtcattat taaagtcctc tctatacata atcttcaaca
    14461 atactattat aatgcaattt ctaccatttt taaaacaata gggcttgatt tttgattaaa
    14521 aatacaaaaa atttttctta aaatgagtta atcacactta gaacactaaa tactattttg
    14581 aggagtccta ttcatcacac tatgccatgt aatattcgga aaaatagtat tgcaaccttc
    14641 aattactcct tatgttggca ccagcctttg aaagtcctct gattgcactg cttagttagt
    14701 attttcatca gctgtatggt ctctaagagc catcagcata ctaaaaaact agtgttacac
    14761 tttgaaaagt gtaacactag ctccaatttg ttttaaatag cttaactatt taatcattaa
    14821 tctactttat atatttaagc attcaaagtt gaatgttggt taaggatacc tgagagagaa
    14881 ccttgaatat aatttggcaa tacttgtttt tgagcacgtt catagatttc tgaatctgtt
    14941 aactgacgtt ctgctttggg gcttgatact tgcggtgtca ctgctgcgtt ttgttgtttg
    15001 taaagtgttg aatgttggtt aaggatacct gagagagaac cttgaatata ttttggcaat
    15061 acttgttttt gggcacgttc atagatttct gaatctgtta actgacgttc tgctttgggg
    15121 cttgatactt gcggtgtcac tgctgcgttt tgttgtttgt aaagtgttga atgctggtta
    15181 aggatacctg agagagaacc ttggatatat tttggtaata cttgtttttg ggcactttca
    15241 taaagttctg aatctgttaa ctgacgttct gctttggggc ttgatacttg cggtgtcact
    15301 gctgcgtttt gttgtttgta aagtgttgaa tgttggttaa ggatacctga aagagaacct
    15361 tggatatatt ttggcaatac ttgtttttgg gcactttcat aaagttctga atctgttaac
    15421 tgacgttctg ctttggggct tgatacttgc ggtgtcactg ctgcgttttg ttgtttgtaa
    15481 agtgttgaat gttggttaag gatacctgag agagaacctt ggatatattt tggtaacact
    15541 tgtttttggg cacgttcata gatttctgaa tctgttaact gacgttctgc ttttggatgt
    15601 gttgcttgcg gtgctgtgaa agttgaagct gaaacacctt gtgtagaacc aagagctacg
    15661 aatgctcctc cagcaaacat tgctgagaca agaccgagtt tcaaatagtt aacagttgag
    15721 acgttgttga cttttttttc tgtaataagt tttttcataa gaattacctc tttcttttgg
    15781 gttatttatt ttatgaacta agtatagcaa caagaaataa agtaaggtta aactaaatat
    15841 aaaataattc ttaagtcatt cttaggaaac cataaaatca tacattaaca aaaaatttgg
    15901 tatctagatc aaaagtaaag gtagaataaa ggcttttact tctgagctag taatgaataa
    15961 attatatttg catctagttt ggaattagcc ctagtgtatt ataatgattc ctacaaatat
    16021 gtgccaaaca ccttcttcat atatacaaga ttgatttaac attcctatac ccttaatggt
    16081 gggcacattc tcttcatgtt ccatatagaa atcaacagat aattccgtat tgtccctatt
    16141 attaaatttt ccagttaatg ataaattata tcctccacct tgaggcgccc tattaccaat
    16201 ctgttcaacc tttacacttg acgaatcgaa ttgaacagat tctatcttat cattctgtga
    16261 ttttagaaca tcaacaattt catattcatg ttctttgagg taagctagtt gttcttcttt
    16321 aacgtgcttt tatctttcgt acttactgat acttgagagc atttatttga cccacttctt
    16381 actaatcaga tgacgtccct ctaagatgaa tcctaaacac gtagctgcca ctaaccattt
    16441 taacctttta tgcatatata attcttcttg atatcctata ccattatctt tagtataaac
    16501 acttaataaa acaagcattt aacaactacg gttaataaaa ttcaataaat ctaatccttt
    16561 tacttacttt agtttatcac ttttttctct catcctatgc tataatagta aggtatcaaa
    16621 atttaggaga atatagtctt atgatgaata tgcaaaatat gatgcgtcaa gcacaaaagc
    16681 ttcaaaaaca aatggaacaa aagcaagctg atttagcagc tagccagttt actggaaaat
    16741 ctgctcaaga gctcgttact gtgacattta caggcgataa aaagctaatt tcaattgatt
    16801 acaaagaagc tgtggtagac cctgaagata ttgaaacact acaagacatg acaactcaag
    16861 ctatcaatga cgctttaagc caagttgacg atgctacaaa aaaaattatg ggagcatttg
    16921 cgggtaaaat gccattctaa aaaataaatc acatttactg aatcacctgt aaatgtgttt
    16981 ttttatttca aatcactttc atttttcatg aaaataagtt ttattttcaa aaaaatattt
    17041 gcaaaaaatc agcagagagg tataataaca ttgaaaatat tagagagaag gtgactaacc
    17101 atggttatgg atagtctaat cataaaacag ttgattaaga gtacatttga tataccctta
    17161 caagtcactt atccaaatgg gaatattgaa acttataatg gtaacaatcc ccacgtcaaa
    17221 cttaaactaa acaaaaattt ctccgtctcg gagctatcaa aagacccttc tattgtattg
    17281 ggtgaagctg ttatggatgg agatattgaa attgatggtt ctatacaaga attaatttta
    17341 tcagcttacc gctgtggaga cagctttcta cgtaatagta aatttagcaa attgattcct
    17401 aaacaatttc atgataaaaa acacagtaaa tccgacattc aaaagcatta tgacatcgga
    17461 aatgatttct ataaactttg gcttgatgat accatgactt attcatgcgc ctattttaaa
    17521 catgaaaatg acagtctaga gcaagctcaa ctaaacaaag tacaccatat tcttaataaa
    17581 ttaaatgctc agccgggagg gaaattacta gatatcggtt gtggctgggg gacacttatc
    17641 attactgctg ctaaagaata tggcttaaat gcaacaggta ttaccttaag tgaagagcag
    17701 gcttccttca ttactaagcg tatcaaggaa gaaggattgg aaaataaagt aacagtcctc
    17761 attaaagatt atcgtgacat tcatgaaaca tacgattata tcactagtgt tggtatgttt
    17821 gaacatgtag gaaaagaaaa tttatctcaa tacttccaaa ctatttctaa aagactcaac
    17881 atcaatggtc tagctcttat tcatggtatt actggacaag tgggtgataa tcatggtagt
    17941 ggaactaatt cttggattaa taagtatatc ttcccaggag gatatatccc tcgtttaact
    18001 gaaaatttaa atcatatagc tagtgctgga cttcaaattg ctgacttaga accgcttcgt
    18061 cgtcactatc aaaagactct cgaattatgg acaaaaaatt tccacaatgc ccttcctgaa
    18121 gttcagaaaa ctcatgataa gcgttttatc aatatgtggg acctctatct tcaatcttgt
    18181 gcggcctctt ttgaatctgg caatattgat atttttcaat acttgctttc aaaaggtgtt
    18241 tcaaaagaca ctatgcctat gacacgcgat tatatgtatt ctgctaattg atatagttaa
    18301 tattatagta ttccaactta ttctaaataa aaagaactct cttgaattca agggagttct
    18361 ttaacgttct tctctggcta actctataca atgtgacttt tggtgacaat gtggacagaa
    18421 tacttttcgc gtcttagggg tatgtttcgc tagattaaat gcttgatatg tcggtgtaaa
    18481 ggtttgatga caatttggac aaagataaac aacctgttgt ttaaaatacc aaaccatacc
    18541 attcatagct attaagaata ctgttactgc tatccacata agccatcttt gatttagaga
    18601 aactgctgct attattaacg taatatagac cataataatc ataacgactt gtagcactat
    18661 gcttttttgt agtttacccc attttctttt gttttcttcc ataagcacaa tgtcagaaag
    18721 atagcctaat gttttactgt cttgtttttc tacggttttt agtaggttga cagctttgtc
    18781 aagtttgtct tttttactat caatagctag ccttaattct cttatctgca cttgtaaaaa
    18841 aagctctaaa atctggctag cattgtcttc agtaaataga tttttgatat tatcaattga
    18901 aaaatctaaa tctcgtaaat aaataatttg tctaagcttt tctaaatcag caatactata
    18961 aaccctccta cctccttcag atagttctga tggggataaa attcctcttt tgtcatagta
    19021 ctgaacagta cgaacagtaa ctcctgcttc tttagctaag tcaccagttg aatatttttc
    19081 tgacatgttt cacacctcct ttaatatagt aaacacttct ttatcagaaa cacctttact
    19141 atatcaaatg acctaaggta ataagcaagt aataacgcta agtattttta actttaaata
    19201 aaactacaac aattaatcaa acaaatttcc taaagccgca aatgggctat caactgctac
    19261 ctctttttgc tgttttaaat agattttatt ttcatccaag tccagaaatt tttcataaac
    19321 acgcgcagtc atgcgggcat cctctaagct attatgtgcc ttacccttta ttcctaaaaa
    19381 gtcagcaaca gtagtcaatt taaggttgac gataccattt aaatctgtac tacgtctcac
    19441 aaaagcttca tcatataagt ccacctgata ttgactagtt agatccaaac cattttcaac
    19501 taaaagaggt aaatccgatt tatgcccatt ataaccaatc aacggtgtgt ctccaacaaa
    19561 actctgcaag tctgtcaaaa ttgtctcaat tttaggagcc cctataatat ctctagctgt
    19621 aatacctgtt aaaccattaa taaagctttg taatggaact tttgtgtgaa cataagtatc
    19681 aaataaagca atctcttgat gattagaata tttaacggcc gacacttgaa taatatggct
    19741 atgctcacca accgtgttaa actctaaatc aaaagctata tattcttgta attgtttcat
    19801 tatttctcct aaaaagcgca aggaaactcc ttacgctttt attctatttt ttattgaaga
    19861 aacgtccaaa gaaacttttt ttaacagctt ctaattcttg aattttttct tctttggctt
    19921 cttgaatctc ttcaacctct gctttagctt catttaactc aagttgcaga cgttgcgtat
    19981 cttccatagc agttaaagtc aattgctgtt gttgatctaa ttgcttatct ttctctgcta
    20041 tttgaatatc ttttacatgt aactgctcgt cttttgaagc aatctgaata tcttttgctt
    20101 ttaactgttc atataaacgt gtgatttcag tattcttctc atcaacgaga atttctaaaa
    20161 gttctcgtct tgatgcttct tcgtcaattg gttcctcttc caaaatagtt tttttataga
    20221 tttcttccaa tttaatcaag ccatcacgat taacaactgt aacgccttta ctattctttt
    20281 ccacacattc ttcagggagt gttttaactc tgttattcat cgcttgacga ctaaccccta
    20341 aaatttcagc taattcacta accgtctttt caactgtcat aacttcctct tttgtactga
    20401 ttttcttata aaagaatatc acaatcctaa tctagtgtca atttactgtc aacttaaagt
    20461 gtcaaacaag acattttgac gtctatttct ctatttatat gctagaataa gattatgaag
    20521 cattatgaca caataattat aggtggtgga cctgctggaa tgatggcagc tatttcctca
    20581 aatttttacg gcaacaaaac acttcttatt gagaaaaata aacgtttagg caaaaaacta
    20641 gctggaacag gtggtggccg ctgtaatgtt accaataatg gaaaccttga tgagctctta
    20701 gctggtatac ctggaaatgg ccgtttccta tatagcgtct tctcacaatt tgataaccat
    20761 gatattatta acttctttca agacaacggc gtcacactta aagaagaaga tcatgggcgt
    20821 atgttcccta ctactgataa atctcgtact atcattaacg cccttgaaaa taaaataaaa
    20881 gaacttggtg gtcaaattat gactgacaca gaagttgtct ctgtcaaaaa gataggagat
    20941 tcattctata tcaaaactaa agatacacag ttcgcatccg ataaacttat cgtaacaact
    21001 ggtggtaaat cctatccgtc tacaggttct actggtttcg gtcatgacat tgctcgtcac
    21061 tttaaattag aagtcacaga tatggaagcc gccgaaagcc ctcttttaac tgattttccc
    21121 cataaaaaac tacaaggtat ctccttagat gatgtcactt tgtcatttga aaagcatatt
    21181 attacacacg atcttctctt tactcatttt ggattatcag gaccagcagc cttgcgaatt
    21241 tccagctttg tcaaaggcgg agaaactata tacctagatg ttttgcccaa tatctctgtt
    21301 aaagaattgg aaacacactt ccaaaatgaa agagaaaaat ctttgaaaaa cgctcttaaa
    21361 atacttcttc ctgagcgttt agctgaattt tacgctgaag acttaccaga aaaagtaaaa
    21421 caagtatctg taaaagactt agaaacgtta attcaaaaac ttaaaaaact tcctatttta
    21481 gtaactggta agatgtctct agcaaaatcc tttgtaacaa aagggggagt tgatcttaaa
    21541 gaaataaacc ccaaaacact tgaaagtaaa aaggtagctg gtttacattt tgctggtgaa
    21601 gtcctagaca tcaatgctca tacgggaggg tttaacatta cttctgcctt atgtacaggt
    21661 tgggtagctg gtagcttaca ctattaatac atgaaagctg aacaattccg tcagcttttt
    21721 tatatagaat ttaaagataa tatttggctc ttaaggcttc tttgattttt tccaattcat
    21781 ctggtaaatt tacctgatga atagcatacc caatctgata aggggatact ataaaacgag
    21841 gaacaatttt acaaataaga cccccatcat gcttatagaa aaagagatta taagatttac
    21901 agcgccctcc caagaattca tttaaaatat aatgacacaa aattggtaaa ctatctgcaa
    21961 tcaaaatagc gttttccaat ttggatactt taattgtgaa ctctgtgaaa tcaagcatag
    22021 gacgattggc aatactaatc tctgcttgtt gactcgtttc cgaacaaacc tcgataccat
    22081 caaaccaatc taagttcaac gtttcataag catctacgtt ttttaagcca acaatttggc
    22141 tatgaggatg tcgtaaactt ccgccagata agggcccaaa atttcgataa agaacgaccg
    22201 aacggtaatg accactttct tccattaatt gccattttga caaaagataa tctaacaatt
    22261 ggcgcatctt tgactcacta taaactgaaa tatccccatt gtgatcattt gattcaatca
    22321 ccaatgtttg ataggtttct tctaaagttg ggtatttatt ttctacccag atcatatcgt
    22381 ctttcacgtc taggattttt cctaattggc ttttatcaca aaatggacat ttgtctttat
    22441 tactgggttt ttgtttcgcg atattagggt taaatcggat atatctttca ttcatactgt
    22501 tattgtaaca cattcatcac cattacaata taatacttat ccagttaaca ttttccttgc
    22561 attcttagct gaaaagagta taatagaatc atgtcttgtt tactggttaa ttaccagtta
    22621 actaggcacc ctgttttggg atagaaaaat ctttttggga aaggagaaat catggctaag
    22681 aaatcaaaaa ttgctaaatt tcaaaaacag caaaaactcg tagaacaata tgctgaacta
    22741 cgtcgtgagt taaaagaaaa gggtgattac gaagcacttc gtaaacttcc taaagattct
    22801 aaccctaacc gacttaaaaa tcgtgattta atcgatggcc gtcctcacgc ttacatgcgt
    22861 aaattcggta tgagtcgtat taacttccgt aacctagctt acaaaggcca aattcctggc
    22921 attaaaaaag caagctggta attcgtgaag acaaatagtt actttttatg actattctat
    22981 tgagtgtgga cgaatagtta atacattgta attatttaac ctgttgagta tttactcagc
    23041 tattacaaac ttcttcaaaa ttttattatc ataggaatgt aggatctcat caatccttaa
    23101 at
//
LOCUS       DBJYSH010000015        22652 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.015, whole genome shotgun sequence.
ACCESSION   DBJYSH010000015 DBJYSH010000000
VERSION     DBJYSH010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 22652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22652
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.015"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            57..731
                     /gene="dltR"
                     /locus_tag="ACUBV3_000484"
     CDS             57..731
                     /gene="dltR"
                     /locus_tag="ACUBV3_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001238594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DltR"
                     /protein_id="HHU3847016.1"
                     /translation="MRLLVVEDEKSIAEAIQALLADKGYSVDLAFDGDDGLEYILTGL
                     YDLVLLDIMLPKRSGLSVLKRVREAGLETPIIFLTAKSQTYDKVNGLDLGADDYITKP
                     FEADELLARIRLRTRQSSLIRANQLRLGNIRLNTDSHELESKESSVKLSNKEFLLMEV
                     FMRNAKQIIPKNQLISKVWGPSDNSEYNQLEVFISFLRKKLRFLKADIEIITTKGFGY
                     SLEERT"
     gene            731..1918
                     /gene="dltS"
                     /locus_tag="ACUBV3_000485"
     CDS             731..1918
                     /gene="dltS"
                     /locus_tag="ACUBV3_000485"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000490537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DltS"
                     /protein_id="HHU3847017.1"
                     /translation="MFSDLRKKFVFLTMSILIVVVLFLFAVSNRYNQYWDEYDAYRIV
                     KLVAKNDYLGIPGDEPIALVTIDNQKMVKIQSNNTDLTNDVIEKSSLKLLEQGKKSRK
                     WKSFIYSIKEYKDKTYTIAIMDLASYEVPYARRFLILVFTIFGFCLLAAVSLYLSRFI
                     VGPVETEMTREKQFVSDASHELKTPIAAIRANVQVLEQQIPGNRYLDHVVSETKRMEF
                     LIEDLLNLSRLDEKRSKVNFKKLNLSVLCQEVLLTYESLAYEEEKCLNDTIEDDVWIV
                     GEESQIKQILIILLDNAIRHSLSKSEIQFSLKQARRKAILTISNPSAIYSKEVMDNLF
                     ERFYQAKDDHADSLSFGLGLSIAKAIVERHKGRIRAYQEKDQLRLEVQLPIDGFWTNT
                     MIN"
     gene            1929..2051
                     /locus_tag="ACUBV3_000486"
     CDS             1929..2051
                     /locus_tag="ACUBV3_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HHU3847018.1"
                     /translation="MKPYLSFIGRTLLYFGILLLLIYFFAYLGRGQGSFIYNEF"
     gene            2064..3599
                     /gene="dltA"
                     /locus_tag="ACUBV3_000487"
     CDS             2064..3599
                     /gene="dltA"
                     /locus_tag="ACUBV3_000487"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047473 - D-alanine [D-alanyl carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HHU3847019.1"
                     /translation="MIHDMIKTIEHFAETQADFPVYDILGEVHTYGQLKVDSDSLAAH
                     IDSLGLVEKSPVLVFGGQEYEMLATFVALTKSGHAYIPVDQHSALDRIQAIMTVAQPS
                     LIISIGEFPLEVDNVPILDVSQVSAIFEEKTPYEVTHSVKGDDNYYIIFTSGTTGLPK
                     GVQISHDNLLSFTNWMISDDEFSVPERPQMLAQPPYSFDLSVMYWAPTLAMGGTLFAL
                     PKTVVNDFKKLFATINELPIQVWTSTPSFADMALLSNDFNSETLPQLTHFYFDGEELT
                     VKTAQKLRQRFPKARIVNAYGPTEATVALSAVAITDEMLETCKRLPIGYTKDDSPTYV
                     IDEEGHKLPNGEQGEIIIAGPAVSKGYLNNPEKTAEAFFQFEGLPAYHTGDLGSMTDE
                     GLLLYGGRMDFQIKFNGYRIELEDVSQNLNKSQYVKSAVAVPRYNKDHKVQNLLAYIV
                     LKEGVRDDFERDLDLTKAIKEDLKDIMMDYMMPSKFIYREDLPLTPNGKIDIKGLMSE
                     VNK"
     gene            3596..4861
                     /gene="dltB"
                     /locus_tag="ACUBV3_000488"
     CDS             3596..4861
                     /gene="dltB"
                     /locus_tag="ACUBV3_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HHU3847020.1"
                     /translation="MIQAFLEKLPHLDVYGNPQYFFYLILAVLPIYIGLFFKKRFALY
                     EIIFSLSFIVMMLTGSTFNQLKSLLAYVVGQSLLVFIYKAYRKRFNHTLVFYVTVCLS
                     IFPLFLVKLIPAISEDGHQSLFGFLGISYLTFRAVAMIIEMRDGVLKEFTLWEFLRFL
                     LFFPTFSSGPIDRFKRFNEDYINIPDRNELLDMLGQAIHYLMLGFLYKFILAYIFGSL
                     IMPPLKELALEQGGVFNWPTLGVMYAFGFDLFFDFAGYTMFALAISNLMGIKSPINFD
                     KPFKSRDLKEFWNRWHMSLSFWFRDFVFMRLVKLLVKNKVFKNRNVTSSVAYIINMLL
                     MGFWHGLTWYYIAYGLFHGIGLVINDAWVRKKKNINKERRLAKKPLLPENKWTYALGV
                     FITFNVVMFSFLIFSGFLDLLWFPQPHNK"
     gene            4876..5115
                     /gene="dltC"
                     /locus_tag="ACUBV3_000489"
     CDS             4876..5115
                     /gene="dltC"
                     /locus_tag="ACUBV3_000489"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005591431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036370 - D-alanyl carrier activity
                     [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HHU3847021.1"
                     /translation="MDIKSEVLAIIDDLFMEDVSSMMDEDLFDAGVLDSMGTVELIVE
                     LESHFNIDIPIAEFGRNDWNTANKIVAGVTELCNA"
     gene            5108..6370
                     /gene="dltD"
                     /locus_tag="ACUBV3_000490"
     CDS             5108..6370
                     /gene="dltD"
                     /locus_tag="ACUBV3_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070394 - lipoteichoic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HHU3847022.1"
                     /translation="MLKRLGKVFGPLVCALLLLVGLYLVFPVSQPHHLGKEKNSAVAL
                     TKAGFKSRVQKVRAFSDPKANFVPFFGSSEWLRFDAMHPSVLAEAYKRPYIPYLLGQK
                     GAASLTQYYGIQQIKGQIKNKKAIYVISPQWFVRKGANKGAFQNYFSNDQTIRFLQNQ
                     TGTTYDRYAARRLLKLYPEASMSDLIEKVADGQKLSNKDKQRLKFNDWVFEKTDAIFS
                     YLPLGKTYNQVIMPHVGKLPKAFSYNHLSRIASQDAKVATRSNQFGIDDRFYQTRIKK
                     HLKKLKGSQRHFNYTKSPEFNDLQLVLNEFSKQNTDVLFVIPPVNKKWTDYTGLDQKM
                     YQKSVEKIKHQLQSQGFNHISDLSRDGGKPYFMQDTIHLGWNGWLELDKHINPFLTEE
                     NSKPNYHINNKFLKRSWAKYTGRPSDYK"
     gene            6545..6937
                     /locus_tag="ACUBV3_000491"
     CDS             6545..6937
                     /locus_tag="ACUBV3_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1310 domain-containing protein"
                     /protein_id="HHU3847023.1"
                     /translation="MKSIKLLSFIVIILLGLGFTVGGLKVTENNKQQELWEIANSKDA
                     KNAYQKWIYAEDKDAFKENAVIKSYDIDKESIKKNPMGGISVRLIINKDPNLYITCNL
                     DKDNQGRLVSQSSHQSPQLTHLLESRGH"
     gene            6937..8229
                     /locus_tag="ACUBV3_000492"
     CDS             6937..8229
                     /locus_tag="ACUBV3_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mbeg1-like protein"
                     /protein_id="HHU3847024.1"
                     /translation="MEKVLTDLDRVNIAKQEYELGSQLDTLVKIMSQDKVLPIGKVAH
                     VQDGGKETGEQIYTITPNGTLDKPEDVKEVTVLFKGSTAPFGGDDWKTDWFKNDIPIA
                     SKLLLKKFGSQSVSHKQGTKQLEQSAHLLKEVMNKYPNAKISVYGHSLGSMNAQYAIA
                     ELDDNQIYRVQGAWIYNGPNLYSILTDKQKEQVDKIKGVVNNYVDSKDKIGIGYPKEG
                     SDNAVGIVKIVDSKAVSGGSWVANFTNQHIWGGYQYNQDGSLKLAEDKFAYKENYSVV
                     HTNVNQRMHAIKAKLSSFKSTGKEVSKNQAIFLDSQQAKVISSGLVTISKEAVEEVKK
                     VEQKAVAKAEELYQSLKVAPTGVTELSPQELEEAYAQSGVSYASIVGEAQTFFSNKIQ
                     AAESISHAYVELENQINKGVEAMLQQDSNLSGEFKQWH"
     gene            8220..8612
                     /locus_tag="ACUBV3_000493"
     CDS             8220..8612
                     /locus_tag="ACUBV3_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3847025.1"
                     /translation="MALIDIIEKQLADTQRKISDLDDAYHHSCCQFEEKLDDLSVRKN
                     KITNMLQETYDAVEYDLRYSNDSSDMMTLNRILDSYHDDLEQAYHKEYYALSAQEEEY
                     RANYIRQRSEHELTFEELQREKKRELMK"
     gene            8622..8903
                     /locus_tag="ACUBV3_000494"
     CDS             8622..8903
                     /locus_tag="ACUBV3_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3847026.1"
                     /translation="MSLMWDVLTGKESVRTLKKQLDKELIHSVYAPRKRSKFHDIITS
                     HHKEIDEVAINTKTAIHKVANQLDEAIKGEFRSKVEESIESNQQKYNHL"
     gene            9085..9867
                     /locus_tag="ACUBV3_000495"
     CDS             9085..9867
                     /locus_tag="ACUBV3_000495"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004536 - DNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HHU3847027.1"
                     /translation="MIKIFDTHTHLNVENFEGKIDEEINLASELGVTKMNVVGFDQDT
                     ISKSLELSSQYAQVYSTIGWHPTEAGSYDDNIESMIISHLENPKVIALGEIGLDYYWM
                     EDPKDIQIEVFKRQIELSKEYNLPFVVHTRDALEDTYEVIKESGVGPFGGIMHSFSGS
                     LEMAQKFIDLGMMISFSGVVTFKKALDVQEAARELPLDKILVETDAPYLAPVPKRGRE
                     NKTAYTRYVVEKIAELRGITVEEVAEATYQNAVRIFRLDEKN"
     gene            9854..10420
                     /gene="rnmV"
                     /locus_tag="ACUBV3_000496"
     CDS             9854..10420
                     /gene="rnmV"
                     /locus_tag="ACUBV3_000496"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HHU3847028.1"
                     /translation="MKKIDIQEVIVVEGKDDTANLRRFYNVDTYETRGSAIDEDDLER
                     IERLHNLRGVIIFTDPDYNGERIRKIIMKAIPTVRHAFLNRDEAKPVSKTKGRSLGVE
                     HASFEDLQKALSKVTQHFDDEDHFDITQADLIRWGFITASDSRKRREYLGSQLRIGYS
                     NGKQLLKRLRLFGVTKAEVEECMEKYEG"
     gene            10424..10873
                     /locus_tag="ACUBV3_000497"
     CDS             10424..10873
                     /locus_tag="ACUBV3_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HHU3847029.1"
                     /translation="MCLICQRIEMIERNENPYFVKEYETGYLVLGDHQYFQDYCLFLS
                     KKHVTELHELPRDWRNQYLSEMADASEIVAKAFRADKMNIESLGNGDAHLHFHLFPRK
                     TGDLRNYGHNGKGPVWWYPFEKMYADSVRATGAEIEKLKEKLLDVLG"
     gene            10900..11772
                     /gene="rsmA"
                     /locus_tag="ACUBV3_000498"
     CDS             10900..11772
                     /gene="rsmA"
                     /locus_tag="ACUBV3_000498"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018030634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HHU3847030.1"
                     /translation="MRIADKTVTRAILERHGFTFKKSFGQNFLTDTNILQKIVDTAEI
                     DKGVNVIEIGPGIGALTEFLAENAAEVMAFEIDDRLIPILADTLARFDNVQVVNQDIL
                     KADLQTQIQAFKNPDLPIKVVANLPYYITTPILMHLIEGKIPFAEFVVMMQREVADRI
                     SAMPNTKAYGSLSIAVQYYMTAKVSFIVPRTVFVPAPNVDSAILKMVRRDQPVVSVQD
                     EDFFFRVSKVAFVHRRKTLWNNLTSHFGKSEDTKAKLEKALEIAKIKPSIRGEALSIP
                     DFASLADALKEVGI"
     gene            11776..12627
                     /locus_tag="ACUBV3_000499"
     CDS             11776..12627
                     /locus_tag="ACUBV3_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative RNA methyltransferase"
                     /protein_id="HHU3847031.1"
                     /translation="MLVDKKWRFEDSASYFACPKCQNPLIKESNSLKCSDNHCFDLSK
                     FGYVNLLGGKKIDEHYDKKSFENRQLVLENGYYNHILEAISKVLENNSQFHSVLDIGC
                     GEGFYSRQLVNKYEKTFLAFDISKDSIQLAAKSDQSRLVKWFVSDLANLPIQDSSIDI
                     ILDIFSPANYKEFRRVLSDDGILVKVVPVAEHVQELREKASQYLKQKDYSNQKILDYF
                     RENFEIISEQKVVQSYNCSQQERQAFIDMTPLLFSVDKTTIDWASISEITVGALIVIG
                     KKRSVSK"
     gene            12783..13655
                     /gene="rsgA"
                     /locus_tag="ACUBV3_000500"
     CDS             12783..13655
                     /gene="rsgA"
                     /locus_tag="ACUBV3_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HHU3847032.1"
                     /translation="MQGRIVKSLAGFYYVESDGVVYQTRARGNFRKKGQIPYVGDWVE
                     FSSQDQSEGYILSIEERKNSLVRPPIVNIDQAVVIMSAKEPDFNANLLDRFLVLLEYK
                     MIQPIIYISKLDLLDDLVVIDDIREHYQNIGYVFCYSQEELLPLLADKVTVFMGQTGV
                     GKSTLLNKIAPELKLETGEISDSLGRGRHTTRAVSFYNVHKGKIADTPGFSSLDYEVD
                     NAEDLNESFPELRRLSHFCKFRSCTHTHEPKCAVKEALTQGQLWQVRYDNYLQFLSEI
                     ENRRETYKKVIKRK"
     gene            13662..14324
                     /gene="rpe"
                     /locus_tag="ACUBV3_000501"
     CDS             13662..14324
                     /gene="rpe"
                     /locus_tag="ACUBV3_000501"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000106179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HHU3847033.1"
                     /translation="MSTNKIAPSILAADYANFANELKRIEETTAEYVHIDIMDGQFVP
                     NISFGADVVSSMRKHSKLVFDCHLMVVDPERYIEDFAQAGADIMTIHVEATKHIHGAL
                     QKIKEAGMKAGVVINPGTPVESLIPILDLVDQILIMTVNPGFGGQAFIPEMMSKVKTV
                     AAWRKEYGHHYDIEVDGGIDNTTIKAAAEAGANVFVAGSYLFKASDLPAQVETLRVAL
                     DD"
     gene            14317..14949
                     /locus_tag="ACUBV3_000502"
     CDS             14317..14949
                     /locus_tag="ACUBV3_000502"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HHU3847034.1"
                     /translation="MTKIALFAGGDLTYFEYDFDYFVGIDRGSLFLLKNGLSLDMAVG
                     DFDSITEDELLYIKHYCSNIVSASAEKNDTDTELALKTIFKEFPEAQVTVFGAFGGRI
                     DHMMSNIFLPSDTDLEPFMSQIRLKDEQNIVTYLPSGKNQVSRIEGMSYVSFMPESES
                     TLQISGAKYELNKSNYFKKKMYSSNEFMTSPIEVELKDGYLIIIYSKDRG"
     gene            14951..16225
                     /gene="rmuC"
                     /locus_tag="ACUBV3_000503"
     CDS             14951..16225
                     /gene="rmuC"
                     /locus_tag="ACUBV3_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000960179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HHU3847035.1"
                     /translation="MLVIILIIVLASLTVTIISYQKMTELTKSVEKQLEDNADNLSDQ
                     LTYQIEAAQKDQILTLTNQLNRMQQEIYQLLTDMRTELNQHLTESRDRSDKRLELINS
                     NLSQSVQKMQDSNEKRLDQMRQTVEEKLEKTLQTRLQTSFETVSRQLESVNQGLGEMK
                     TVAQDVGTLNKVLSNTKTRGILGELQLGQIIEDIMTVSQYEREFPTVSGSSERVEYAI
                     KLPGNGQGDYIYLPIDSKFPLEDYYRLEDAYELGDKVQIELYRKSLLASIRKFAKDIN
                     NKYLNPPETTNFGIMFLPTEGLYSEVVRNATFFDSLRRDENIVVAGPSTLSALLNSLS
                     VGFKTLNIQKNANDISKILGNVKVEFGKFGGMLSKAQKQLNTASKSIDNLLTTRTNAI
                     IRALNTVEEHQDQATTSLLNLPITEEEEINEN"
     gene            16215..17156
                     /locus_tag="ACUBV3_000504"
     CDS             16215..17156
                     /locus_tag="ACUBV3_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM family protein"
                     /protein_id="HHU3847036.1"
                     /translation="MKINQMKKDELFEGFYLIKKAEVRKTRAGKDFIAFTFQDDTGEI
                     SGNMWDAQTYNVEEFVAGKIVHMKGRREVYNGTPQVNQITLRNIKDGEPNDPRDFKEK
                     PPINVDNVREYMEQMLFKIENATWQRVVRALYRKYNKEFFTYPAAKTNHHAFESGLAY
                     HTATMVRLADSIGDIYPELNKSLMFAGIMLHDLAKVIELSGPDNTEYTIRGNLIGHIS
                     LIDEELTKILAELNIDDTKEEVTVLRHVILSHHGQLEYGSPVRPRIMEAEIIHMIDNI
                     DANMMMMTTALNRVNEGEMTNRIFAMDNRSFYKLNIK"
     gene            17253..18065
                     /gene="purR"
                     /locus_tag="ACUBV3_000505"
     CDS             17253..18065
                     /gene="purR"
                     /locus_tag="ACUBV3_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HHU3847037.1"
                     /translation="MKLRRSERMVVISNYLINNPYTLTSLNTFASKYGAAKSSISEDI
                     AIIKKAFEQAQIGDIKTVTGASGGVIFTPTIAEAEAKEIVEELRQRLSENDRILPGGY
                     IYLSDLLSTPKMLQSIGRIVANAYRGQKIDAVMTVATKGVPLANAVANVLDVPFVIVR
                     RDLKITEGSTVSVNYASGSSGRIEKMFLSKRSLKPNSRVLIVDDFLKGGGTVSGMISL
                     LSEFDSTLVGVAVFAENAQEQREKMAYKSLLRVSEIDVKNNRVSVEAGNIFD"
     gene            18284..18697
                     /gene="rpsL"
                     /locus_tag="ACUBV3_000506"
     CDS             18284..18697
                     /gene="rpsL"
                     /locus_tag="ACUBV3_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HHU3847038.1"
                     /translation="MPTINQLVRKPRKSKVEKSDSPALNIGYNSHRKVHTKLSAPQKR
                     GVATRVGTMTPKKPNSALRKFARVRLSNLIEVTAYIPGIGHNLQEHSVVLIRGGRVKD
                     LPGVRYHIVRGALDTAGVADRKQGRSKYGAKRPKG"
     gene            18719..19189
                     /gene="rpsG"
                     /locus_tag="ACUBV3_000507"
     CDS             18719..19189
                     /gene="rpsG"
                     /locus_tag="ACUBV3_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HHU3847039.1"
                     /translation="MSRKNQAPKREVLPDPLYNSKIVTRLINRVMLDGKRGTAATIVY
                     DAFEAIKEATGTDALEVFETAMENIMPVLEVRARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVNASRARGEHTMKDRLAKEIMDAANNTGASVKKREDTHKMAEANRAFAHFRW"
     gene            19344..21422
                     /gene="fusA"
                     /locus_tag="ACUBV3_000508"
     CDS             19344..21422
                     /gene="fusA"
                     /locus_tag="ACUBV3_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HHU3847040.1"
                     /translation="MAREFSLEKTRNIGIMAHVDAGKTTTTERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWDGHRVNIIDTPGHVDFTIEVQRSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATEYGVPRIVFANKMDKIGADFLYSVQSLHDRLQANAH
                     PIQLPIGSEDDFRGIIDLIKMKAEIYTNDLGTDILEEDIPAEYVDQANEYREKLVEAV
                     ADTDEDLMMKYLEGEEITNEELMAAIRRATINVEFYPVLCGSAFKNKGVQLMLDAVID
                     YLPSPLDIPAIKGINPDTDEEETRPASDEEPFAALAFKIMTDPFVGRLTFFRVYSGVL
                     NSGSYVLNTSKGKRERIGRILQMHANSRQEIETVYAGDIAAAVGLKDTTTGDSLTDEK
                     SKVILESIEVPEPVIQLMVEPKSKADQDKMGIALQKLAEEDPTFRVETNVETGETVIS
                     GMGELHLDVLVDRMKREFKVEANVGAPQVSYRETFRASTQARGFFKRQSGGKGQFGDV
                     WIEFTPNEEGKGFEFENAIVGGVVPREFIPAVEKGLVESMANGVLAGYPMVDVKAKLY
                     DGSYHDVDSSETAFKIAASLALKEAAKSAQPAILEPMMLVTITAPEDNLGDVMGHVTA
                     RRGRVDGMEARGNTQVVRAFVPLAEMFGYATVLRSATQGRGTFMMVFDHYEDVPKSVQ
                     EEIIKKNSGE"
     gene            21626..22636
                     /gene="gap"
                     /locus_tag="ACUBV3_000509"
     CDS             21626..22636
                     /gene="gap"
                     /locus_tag="ACUBV3_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HHU3847041.1"
                     /translation="MVVKVGINGFGRIGRLAFRRIQNVEGVEVTRINDLTDPNMLAHL
                     LKYDTTQGRFDGTVEVKEGGFEVNGQFVKVSAEREPANIDWATDGVEIVLEATGFFAS
                     KEKAEQHIHENGAKKVVITAPGGNDVKTVVFNTNHDILDGTETVISGASCTTNCLAPM
                     AKALQDNFGVKQGLMTTIHAYTGDQMILDGPHRGGDLRRARAGAANIVPNSTGAAKAI
                     GLVIPELNGKLDGAAQRVPVPTGSVTELVATLEKDVTVEEVNAAMKAAANDSYGYTED
                     PIVSSDIVGISYGSLFDATQTKVQTVDGNQLVKVVSWYDNEMSYTSQLVRTLEYFAKI
                     AK"
BASE COUNT         7493 a         3682 c         4581 g         6896 t
ORIGIN      
        1 ggttatttca attttgctag tttatactat aatatataag gtaaggtgac tagaaaatga
       61 gacttcttgt agttgaggat gaaaaaagta tagcagaggc tattcaggct ttattggcag
      121 ataaaggtta ttctgttgat ttagcatttg atggagatga tgggttagaa tacattttga
      181 caggtctcta tgatttagtc ctgttggata ttatgttacc caaacgctca ggattatctg
      241 ttttaaaaag agtgagggaa gctggtttag aaaccccaat aatcttttta actgcaaaat
      301 cacagactta tgataaagtt aatggtttag atttaggggc agatgattat atcacaaaac
      361 cctttgaagc agatgaattg ctagctagaa ttaggctgag aaccagacaa tcttctctaa
      421 tcagagcaaa tcaattgcgg cttggaaata taaggcttaa cacagattca catgagctag
      481 aatctaaaga aagtagtgta aaattatcca ataaagaatt tctcttgatg gaagttttta
      541 tgcgaaatgc aaagcagatt attcctaaaa atcagttgat ttctaaggtt tggggacctt
      601 cagacaattc agaatataac cagttagaag tatttatttc ttttttgcga aaaaaattgc
      661 gctttttaaa agcagacatt gaaataataa ccactaaggg ctttggatat tccttggagg
      721 agaggacata atgtttagtg atttacgcaa aaaatttgtc tttttgacca tgtcaatcct
      781 tattgtcgtt gttctctttc tctttgcagt gagtaatcgc tacaatcaat attgggatga
      841 atatgatgca tataggattg ttaaattagt tgccaaaaat gattatttag gaataccagg
      901 cgatgaaccg attgctttgg tgactataga taaccaaaag atggttaaga ttcaaagcaa
      961 taatactgat ttgacaaatg atgttatcga aaaatctagt ttgaagctcc ttgagcaagg
     1021 aaagaagtca cgaaaatgga agtcttttat atattccata aaagaatata aagataaaac
     1081 ctacaccata gctattatgg atttggctag ttatgaagtc ccttatgcaa ggcgttttct
     1141 aattcttgtt tttactattt ttggattttg tttattagca gctgtaagtc tttatctttc
     1201 tcgatttatc gtagggcctg tagagacaga aatgactcgc gaaaaacagt tcgtttcaga
     1261 tgcgagtcat gagctgaaga cccctattgc agctatacga gccaacgttc aggttctgga
     1321 acagcaaatt ccaggaaatc gttatctgga tcatgttgtt tcggagacta agcgaatgga
     1381 gtttttaatt gaagatttgc ttaatttgtc tagacttgat gaaaaacgct ccaaggtaaa
     1441 tttcaaaaag ttaaacttat ctgtcttatg tcaagaagta cttctgacat atgaaagttt
     1501 ggcatatgaa gaagaaaagt gtttaaatga tacgattgaa gatgatgtct ggattgttgg
     1561 agaagagagt cagattaagc aaattttaat aattcttttg gataatgcta ttaggcattc
     1621 tttgtcaaaa tcagaaatcc agtttagtct taaacaggct cgaagaaaag ctattctgac
     1681 aatatcaaat ccaagtgcta tctattccaa agaggttatg gataatctct ttgagcgttt
     1741 ttatcaagct aaagatgatc atgcagattc tctcagtttt ggtctaggct tatctattgc
     1801 taaggcaatc gtggaacgcc ataaggggag aattagagca tatcaagaaa aagaccaatt
     1861 acgtcttgaa gtccagttac caattgatgg attttggacc aataccatga ttaattaaag
     1921 gaagaatgat gaaaccatat ttatctttta ttggtagaac gttattatac ttcggtattt
     1981 tattgttact aatttacttt tttgcatacc ttggtcgcgg acaaggcagt tttatttata
     2041 atgaattcta ggagatggta caaatgatac atgatatgat taaaacaatt gagcattttg
     2101 ctgagacaca agctgatttt ccagtgtatg atattttagg ggaagtccat acttatggac
     2161 aacttaaagt agactctgac tctctagctg ctcatattga tagcctaggt cttgttgaaa
     2221 aatcacctgt cttagtattc ggtggtcaag aatatgaaat gttggcgaca tttgttgctt
     2281 taacaaagtc agggcatgct tatataccgg ttgaccaaca ctctgctttg gatagaatac
     2341 aggctattat gacagttgct caaccaagcc ttatcatttc aattggtgaa tttcctcttg
     2401 aagttgataa tgtcccaatc ctagacgttt ctcaagtttc agctattttt gaagaaaaga
     2461 ctccttatga ggtaacacat tctgttaaag gtgatgataa ttactatatt attttcactt
     2521 cagggactac tggtttacca aaaggtgtgc aaatttcaca tgacaattta ttgagcttta
     2581 caaattggat gatttctgat gatgagtttt cagttcctga aagaccgcaa atgttggctc
     2641 aaccgccata ttcatttgac ttatcagtta tgtattgggc accaacacta gctatgggag
     2701 gcaccctgtt cgccctacca aaaacagtgg ttaatgattt caaaaaacta ttcgctacca
     2761 ttaatgaatt gccaatacag gtttggactt cgacaccatc atttgctgat atggcgctac
     2821 tatctaacga tttcaattca gagaccttgc cacaattaac acatttttat tttgatgggg
     2881 aagagttaac tgtcaagact gcacaaaaac ttcgtcaacg ttttccaaaa gctcgtatcg
     2941 ttaatgcata tgggccaaca gaagcaacag ttgctctatc cgcagtagca attactgatg
     3001 aaatgttaga aacatgcaaa cgccttccaa ttggttacac taaagatgac tcaccaacgt
     3061 atgtgattga tgaagaaggt cataaattac caaacggaga gcaaggtgaa atcattattg
     3121 ctggaccagc agtatcaaaa ggctatctta acaatccaga aaagacagct gaggcatttt
     3181 tccaattcga aggtctacct gcttaccata ccggtgactt aggaagtatg accgatgaag
     3241 gtcttctgct ttacggtggg cgtatggatt tccaaattaa atttaacggc tatcgtattg
     3301 aattagaaga tgtttctcaa aacttaaata aatcgcagta tgtaaaatca gcagtagcag
     3361 tgccacgtta taacaaggat cataaagttc aaaatttatt agcctatatt gtcttaaaag
     3421 aaggtgtaag agatgatttt gaacgtgatt tggatttgac aaaagcaatt aaggaagact
     3481 taaaggacat tatgatggat tacatgatgc catctaaatt tatctatcga gaagatttac
     3541 ctttgacacc aaatggaaaa attgatatca aaggtcttat gagcgaggta aacaagtgat
     3601 acaggcattt ttagaaaaac ttcctcactt ggatgtttat gggaatccac aatatttctt
     3661 ctaccttatc ttagctgttc taccaattta catcggctta ttctttaaga agcgttttgc
     3721 cttatatgag attattttta gtctaagttt tattgtaatg atgttgactg gtagtacttt
     3781 taatcaattg aagtcactat tggcatacgt tgtcggacaa tctctgctag tttttatcta
     3841 taaagcttac cggaaacgat ttaatcatac tttggtcttt tatgtaacgg tttgtttatc
     3901 tatttttccg ctatttttgg taaaattaat tccagctata tctgaggatg ggcatcagtc
     3961 actttttggg tttctaggaa tttcttacct tacttttaga gctgttgcta tgattattga
     4021 aatgagagac ggtgtcttga aagaatttac tttatgggaa ttcttaagat ttttactctt
     4081 ctttccaact ttctcaagtg gaccaattga tcgttttaaa cgattcaatg aggattacat
     4141 taatatccca gatcgaaatg aactcctaga tatgttaggt caagcgattc attatttgat
     4201 gttaggtttt ctctataagt ttattttagc ctatattttt ggaagtctga ttatgcctcc
     4261 tctaaaagaa ttagcactag aacagggtgg tgtgtttaat tggccaacac ttggggttat
     4321 gtatgccttt ggttttgatt tgttctttga ttttgcaggt tacacaatgt ttgcgttggc
     4381 tatttctaac ctaatgggga ttaagtctcc gattaacttt gacaaacctt tcaaatcacg
     4441 cgacctaaaa gaattttgga atagatggca tatgagcctt tctttctggt ttagagactt
     4501 tgttttcatg aggcttgtta agcttttagt taaaaataaa gtttttaaaa accgtaatgt
     4561 tacttcaagt gtagcttata ttatcaatat gcttcttatg ggattctggc atgggttaac
     4621 ttggtactat atagcctatg gtctctttca tgggattggc ctagttatta atgacgcttg
     4681 ggtacgtaag aagaaaaata ttaataaaga aagaagattg gctaaaaaac cacttttacc
     4741 agaaaacaaa tggacttatg ctttgggtgt cttcatcacc tttaatgtag ttatgttttc
     4801 tttcttgatt ttttcaggat ttttagatct tttgtggttc ccacaaccgc ataacaaata
     4861 ataaggagta ttataatgga tattaaatca gaagtattag caattattga tgacctattt
     4921 atggaggatg tttcttctat gatggatgaa gacttatttg acgcaggagt tctagacagc
     4981 atgggtactg ttgaactaat tgttgagttg gaaagccact ttaatattga tattccgatt
     5041 gcggaatttg gtcgtaatga ctggaacaca gctaataaaa ttgttgctgg agtaacggag
     5101 ctctgcaatg cttaaacgtc taggtaaagt atttggacct ctagtttgtg ctttactatt
     5161 gttggtagga ttatatcttg tttttcctgt ttctcagcct catcatttag gtaaggaaaa
     5221 aaacagtgca gtagcgttga caaaggcagg ttttaaaagc agagttcaaa aagttagagc
     5281 tttcagtgat cctaaagcca attttgtccc tttctttggt tcaagtgagt ggttaagatt
     5341 tgatgcaatg catccatcag ttttagcaga ggcttacaaa aggccttata tcccatatct
     5401 tttaggtcaa aaaggggcgg cttctctgac acaatactat ggcattcaac agattaaagg
     5461 acaaatcaaa aataaaaaag ctatctatgt tatttctccg caatggtttg ttcgcaaggg
     5521 agccaacaaa ggtgcttttc aaaactattt cagcaacgat caaaccattc gatttttgca
     5581 aaatcaaaca gggacaacct acgataggta tgctgctcgt cgattgttaa aattatatcc
     5641 tgaagcttct atgtcagatt tgatagaaaa agttgcagat ggccaaaaac tatcaaataa
     5701 agacaaacaa agactaaagt ttaatgattg ggtatttgag aagacagatg ctatttttag
     5761 ctatctacca ctaggaaaaa cttataatca ggtaataatg cctcatgttg gtaaattacc
     5821 gaaagcattc tcatataatc atttatcgcg tattgcatca caagatgcta aagtagcaac
     5881 gagatcaaat caatttggta ttgatgatcg cttttaccaa acaagaatta aaaagcactt
     5941 aaaaaaattg aaagggtcac aacgacattt caattatact aagtcaccag aatttaatga
     6001 tttacagttg gttcttaatg aattctcaaa acaaaataca gatgtccttt ttgtcatacc
     6061 accagtaaat aaaaagtgga cagactacac agggcttgat caaaaaatgt atcaaaaatc
     6121 tgtagaaaaa ataaaacacc aacttcaaag tcaaggtttc aatcatatct ctgacctttc
     6181 tcgagatgga ggtaagccat actttatgca agatacaatc catttaggtt ggaatggttg
     6241 gttagagcta gataagcata tcaatccatt tttaacagag gaaaacagca agccaaatta
     6301 tcacattaat aataaatttt tgaagagatc ttgggcaaaa tatacaggac gtccaagtga
     6361 ttacaagtaa atagtagagt cagatgattt atagcattag agctattaat catctttttt
     6421 aatatctttg tatttggtaa tcctaagata actaatccat gttttatagt ttttcgttta
     6481 taatgagaat aaatttatga taaactagat atcaaatgag tgttatatga taaaaggagg
     6541 agagatgaag tcaataaaat tacttagttt tattgtaata atccttttag ggcttggttt
     6601 cactgtagga ggtttaaaag tgactgaaaa caataaacaa caagaattat gggaaatagc
     6661 taatagtaaa gacgctaaaa atgcttatca aaagtggatt tatgcagaag ataaagacgc
     6721 tttcaaagag aatgctgtta ttaagtcgta tgatattgac aaagaaagta tcaaaaaaaa
     6781 tcctatggga gggatttcgg taaggttaat cattaataaa gatcctaact tatatattac
     6841 ttgtaatttg gataaggata atcaagggcg tttggtcagt caaagtagcc atcaatctcc
     6901 ccagttaact catttattgg aaagtagagg tcattaatgg aaaaagtctt gactgattta
     6961 gatagggtaa atatagcgaa gcaagagtat gaattaggta gccaacttga tacactagtc
     7021 aaaataatga gccaagataa ggtacttccc atcggtaaag ttgctcacgt tcaagatgga
     7081 ggcaaagaaa ctggggaaca aatttatacc atcactccaa atggaacttt agacaagcca
     7141 gaggatgtta aggaagttac ggttttattt aaggggtcaa cagcaccgtt tggaggagat
     7201 gattggaaaa cagactggtt taaaaatgat attccaatag ctagtaaact attattaaag
     7261 aaatttggtt cacaaagtgt atctcataaa caaggtacaa agcagttaga acaatcagct
     7321 catctgctaa aagaagttat gaataaatat cctaatgcaa aaatttcggt ctacggacac
     7381 tcgttagggt caatgaatgc acagtatgct attgcagaat tggatgataa tcaaatttac
     7441 cgagttcaag gagcttggat atataacggt ccaaaccttt atagtatttt gacagataaa
     7501 caaaaagaac aggttgataa aataaaaggg gtagtcaata attacgttga ttccaaggat
     7561 aaaattggaa taggttatcc taaagaagga agcgataatg ctgtcggaat tgttaaaata
     7621 gttgacagta aagctgtttc aggaggtagc tgggttgcta attttaccaa tcagcacatt
     7681 tggggaggct accagtacaa tcaagatggt tctttaaaat tagcagaaga taaatttgct
     7741 tacaaagaaa actattcagt agttcatacg aatgtgaatc aaagaatgca tgctattaaa
     7801 gcgaaactta gtagctttaa aagtacggga aaagaagtta gtaagaatca agctattttc
     7861 ctagatagtc aacaagcaaa agtgatttct agtggtttgg taacgatatc aaaagaagca
     7921 gttgaagaag ttaaaaaagt tgagcaaaaa gcagttgcta aagcagaaga gctttatcaa
     7981 tctttaaaag tagctcctac aggagttact gaactaagtc ctcaagaatt ggaagaagcg
     8041 tatgctcagt caggtgttag ctatgcgagc attgtcggag aagctcaaac ctttttctca
     8101 aataaaattc aagcggcaga aagcatttct cacgcttacg ttgaattaga aaaccaaatt
     8161 aataaagggg ttgaagctat gttgcaacaa gatagtaact tgtcagggga gtttaaacaa
     8221 tggcactgat tgatatcatt gaaaaacaat tagctgatac tcagagaaaa atatcagatt
     8281 tagatgatgc ctatcaccat agttgttgcc agtttgaaga aaaattagac gatctctcag
     8341 taagaaaaaa taagattact aatatgcttc aagaaactta cgacgctgtt gaatatgatt
     8401 tgagatatag taatgatagc tcagatatga tgacgctcaa tcgtatttta gattcttatc
     8461 atgacgactt agagcaagct taccataaag agtactatgc cttgtccgct caagaagaag
     8521 aatatagagc taactatatt cggcaacgtt cagaacatga actaaccttt gaagagttgc
     8581 aaagggaaaa gaaaagagaa ttaatgaagt aggagaaatt attgagttta atgtgggatg
     8641 tcctgactgg taaggagtca gtaaggactt taaaaaaaca attggataaa gaactgatac
     8701 atagcgtgta cgcgccacgc aagagatcta agtttcacga tattattacg tcgcatcata
     8761 aagaaataga cgaagtagcc atcaacacaa agacagctat tcataaagtt gctaatcaat
     8821 tggacgaggc aataaaagga gaatttcgtt caaaggttga ggagtctatt gaaagcaatc
     8881 agcaaaaata taatcattta tagtctagtt tctctaccat tagctagaca cttgtagagg
     8941 atttttacag caagtattca aaagtcagag caaaaatgtt ttgacttttt tgatttactc
     9001 tttctgatca gaatgcttca gtcttatgtt ataataataa agattaatgc ttgtagctac
     9061 gataagcaaa gaaagaacaa tgatatgata aaaatatttg atacccatac ccaccttaac
     9121 gtggaaaatt ttgaaggaaa gatagacgag gaaattaatt tagctagcga actcggtgtt
     9181 acaaaaatga atgttgtcgg ctttgaccaa gataccattt caaaatcttt ggaattatct
     9241 tcccaatatg cacaggtata tagcacaata ggatggcatc caactgaagc tggttcttat
     9301 gatgataata ttgagtcaat gattatcagc catttagaaa atcccaaggt tattgcactt
     9361 ggtgaaattg gtctggatta ttattggatg gaggatccca aagatattca gatcgaggtg
     9421 tttaagcgtc aaattgaact ttcaaaagag tataaccttc cttttgtcgt tcatacacgt
     9481 gatgcgttag aagacactta tgaagtcatt aaggaatctg gagttgggcc ttttggtggt
     9541 ataatgcact ctttttctgg ttctttggaa atggctcaga aatttattga tttagggatg
     9601 atgatttcct tttctggtgt tgtgaccttt aagaaggcat tagatgtaca agaagctgca
     9661 cgagagttgc cattagataa gatattagtg gaaacagatg ctccttattt ggctcctgta
     9721 cctaaaagag gtcgagaaaa caaaactgct tatacgagat atgtcgttga aaaaatagca
     9781 gagctacgag gcataactgt ggaagaggta gcggaggcta cataccaaaa tgcagtaagg
     9841 atatttagac ttgatgaaaa aaattgatat tcaagaagtg attgttgtcg aagggaaaga
     9901 tgatactgct aatctgcgac gcttttacaa tgttgatacc tatgaaacta gaggttcagc
     9961 aattgatgaa gatgatttag agcgtatcga acgtttgcac aatttgcggg gagttatcat
    10021 ctttaccgac ccagattaca atggcgaacg tatccgtaaa atcatcatga aggccattcc
    10081 gacagtccgc catgccttcc tcaatcgaga cgaggcaaag ccagtttcaa agaccaaggg
    10141 acgatctctg ggtgtcgagc atgccagctt tgaggatttg cagaaggcac tctcaaaagt
    10201 gactcagcat ttcgatgatg aagatcattt cgacatcacc caagccgacc tcattcgctg
    10261 ggggttcatt accgctagtg atagccgcaa acgtcgtgag tatctgggta gccaacttcg
    10321 catcggctac tcaaatggta aacaactcct caaacgcctc agactcttcg gagtgaccaa
    10381 ggcagaagtt gaagagtgta tggagaaata cgagggataa aatatgtgtc ttatttgtca
    10441 acgtattgaa atgattgaac gaaatgagaa tccttatttt gtgaaagaat atgaaacggg
    10501 ttatcttgtt ctgggagacc atcaatattt tcaagattat tgcctttttc tctctaaaaa
    10561 acacgtaaca gagttacatg aattgcctag agattggcga aatcagtatc tttccgaaat
    10621 ggcagatgct tctgaaatag ttgctaaggc ttttagagca gataagatga atattgagag
    10681 tttgggaaat ggagacgctc atctacattt tcatcttttt ccaagaaaaa caggtgattt
    10741 aaggaactat gggcataatg gaaaaggtcc agtttggtgg tatccttttg agaagatgta
    10801 tgcagatagt gtcagagcaa ctggtgcaga aatagaaaaa ttaaaagaga aattgttaga
    10861 tgtcttagga tagacttgag acaataagga aagaaacaaa tgagaatcgc agataaaacc
    10921 gttacccgcg ctattttgga gcgtcatggg tttacattta agaaatcatt cggtcagaac
    10981 tttttgactg atactaatat tttacagaag attgttgaca cggcagagat tgacaagggt
    11041 gtcaacgtga ttgagattgg tcctggtatc ggggctttga cagagttttt ggctgagaat
    11101 gcagctgagg tgatggcttt tgagattgat gatcgtctca ttcctatcct tgctgatacg
    11161 cttgcgcgtt ttgacaatgt ccaagtggtc aatcaggaca tcctcaaggc tgatttgcag
    11221 acccagattc aggctttcaa gaatcctgac ctgcctatca aggtagtagc aaacttacct
    11281 tactacatca caacaccaat cctcatgcac ttgattgagg gcaagattcc ttttgcggag
    11341 tttgtagtca tgatgcaaag agaagtggca gatcgtattt cagctatgcc aaatactaaa
    11401 gcatacggtt ctttgtcaat cgcagtgcag tattatatga ctgccaaagt atcatttatc
    11461 gttccgagaa ctgtttttgt tcctgctcca aatgtagatt cagctatttt gaaaatggtc
    11521 cgtagagatc aaccagtagt gagtgttcaa gatgaggatt tctttttcag agtgtcaaaa
    11581 gtagccttcg tacatcgtcg taaaacactt tggaataatc taacgagtca ttttggaaaa
    11641 tcagaagata ctaaagcaaa attggaaaaa gcattagaaa tagcaaaaat taagccatct
    11701 attcgcggag aagccttatc tatccctgat tttgcaagtt tagcagatgc cttgaaagag
    11761 gttgggatat aaactatgtt ggttgacaag aagtggcgtt ttgaagatag tgcaagttat
    11821 ttcgcttgtc ctaaatgtca aaatcccttg ataaaagaaa gtaacagcct caaatgctct
    11881 gataatcatt gttttgattt atccaaattt ggatatgtta atcttcttgg aggaaagaag
    11941 atagatgaac attatgataa aaaatcattt gaaaatcggc agcttgtctt agaaaacggg
    12001 tattataatc atattttaga agctatttca aaggttttgg aaaataattc tcagtttcat
    12061 tcggttttag atattggttg tggagagggc ttttattctc gtcaattagt taacaagtat
    12121 gagaagactt tcttggcttt tgatatttct aaagattcga ttcaattagc tgctaaaagt
    12181 gatcaatcta ggcttgtgaa atggtttgtt tctgatttag ctaaccttcc tattcaagac
    12241 tcatcaattg atatcatttt agacattttt tctcctgcaa attataaaga atttaggcgg
    12301 gtactgagtg atgatggtat ccttgtaaag gttgttcctg tagctgaaca tgtccaagaa
    12361 ttgcgagaaa aagctagtca atacttgaaa cagaaagatt actctaatca aaaaattcta
    12421 gattacttta gagaaaattt tgaaattatt tctgagcaga aagttgttca aagttataac
    12481 tgttctcaac aagaacgtca agcttttatt gatatgacac cgttgttatt cagcgtagat
    12541 aaaacgacta ttgattgggc gagtatatca gaaattacag taggtgcctt aatagtaatt
    12601 ggaaaaaagc gcagtgtatc taaataagat cactgctttt atgttgggaa ttcataaaat
    12661 tgaggttatt tgagagaaat cgaacacctg caaatagttt ctatttagct tatctctgag
    12721 agtagagctt atttctcatt ttatgatata atagtggtat tctaaacaat taaggagaac
    12781 aattgcaagg tagaattgtc aagtcgctag caggttttta ttatgtggaa tctgacggtg
    12841 ttgtttatca gacacgtgcg cgtggaaatt ttagaaaaaa aggtcaaatt ccctacgttg
    12901 gggattgggt ggaattttct tcgcaagatc aatcagaagg atatattcta tcaattgaag
    12961 agcgtaaaaa tagcctcgta cgaccaccta ttgttaatat tgaccaagct gtcgttatca
    13021 tgtctgctaa agaacctgat tttaacgcta acttattaga ccggttctta gtacttctag
    13081 aatataagat gattcagcct atcatttata tttccaagtt ggatttgtta gacgatttgg
    13141 ttgttattga tgatattcgc gaacattatc agaatatagg ctacgttttc tgttattctc
    13201 aagaggaact cttgcctctg ttagcagata aagtgactgt ttttatgggg cagacaggtg
    13261 tcggaaaatc aacgcttctc aacaaaattg ctcctgagtt gaaacttgaa actggtgaaa
    13321 tttcggatag tttaggccgt ggccgacaca ccacaagagc agttagtttc tataatgttc
    13381 ataaaggaaa aattgcagac actcctggtt tttcttcttt ggactatgaa gtggacaatg
    13441 cagaggactt aaacgaatcc tttccagagc tccgtagact tagtcatttt tgtaaattcc
    13501 gctcgtgtac gcatacacat gagcctaaat gtgctgtaaa agaagccttg actcaaggtc
    13561 agttatggca agtccgctat gataactacc tccaattttt aagtgaaatt gagaaccgtc
    13621 gtgaaacata taagaaagtt attaaaagaa agtaggtttc tatgtcaacc aataaaattg
    13681 ccccatcgat attagcagca gactatgcta attttgctaa cgaattgaaa cgtattgaag
    13741 agacaacagc agaatacgtt catattgata tcatggatgg tcaatttgtc cctaatatta
    13801 gttttggtgc ggatgttgta tccagcatgc gtaaacacag taaacttgtt tttgattgtc
    13861 atctgatggt tgttgatcca gaacgttata ttgaggattt tgctcaagct ggtgctgata
    13921 tcatgaccat tcatgttgaa gcaacgaaac acatacatgg tgcgcttcaa aagataaaag
    13981 aggcaggaat gaaagcaggt gtggttatta acccaggaac acccgtagaa tctcttattc
    14041 ctatcttaga tttggttgac cagattttaa ttatgactgt taatcctgga tttggtggac
    14101 aagcctttat tccagaaatg atgtcaaaag taaagacagt tgcagcatgg cgcaaagagt
    14161 acggtcatca ttatgatatt gaagttgatg gcggtataga taatactaca attaaggcag
    14221 cagcagaggc tggtgcaaat gtctttgttg caggctctta tctctttaaa gcaagtgatt
    14281 tacctgcaca agtagaaacc ttaagggtgg cgctcgatga ctaaaattgc tttatttgct
    14341 ggcggtgact taacttattt tgaatatgat tttgactatt ttgtcggaat tgaccgtgga
    14401 agcctatttt tattaaaaaa tgggctttct ttggacatgg cagtgggtga ttttgattct
    14461 ataacagaag atgaactgct ttatatcaaa cactattgta gcaatatcgt ttctgctagt
    14521 gcagaaaaaa atgatacaga tacagaatta gctttaaaaa ctattttcaa agaatttcca
    14581 gaagctcagg tgaccgtatt tggagcattt ggtggcagga ttgaccatat gatgtctaat
    14641 atctttttac caagtgacac agatctagaa ccatttatgt cacaaatcag actcaaagat
    14701 gaacaaaaca tagtgactta cctaccatcc gggaaaaatc aagtctcaag gattgaaggg
    14761 atgtcctatg tctcgttcat gcctgagagt gagtctactt tgcagatttc tggggctaaa
    14821 tatgaattga acaaaagtaa ttattttaag aaaaaaatgt attccagtaa tgaatttatg
    14881 acttcgccaa ttgaagtaga gctcaaagat ggttacttaa tcattattta tagtaaggat
    14941 agaggttaat atgcttgtca tcattttgat cattgtacta gctagtctga cagtgacgat
    15001 aatttcttac caaaaaatga cggaattaac aaagtccgtt gaaaaacaac ttgaagataa
    15061 tgctgataat ctatcagacc aactgacata tcagatagaa gcggcgcaaa aagatcaaat
    15121 tctaacgttg actaatcagc taaaccgtat gcaacaggaa atttatcagt tgcttaccga
    15181 tatgcgtaca gaattaaatc agcatttaac ggagagtcga gatcgatcag acaaacgctt
    15241 ggaattgatt aactccaatt tatctcagtc agttcagaaa atgcaagatt ccaatgaaaa
    15301 acgcttggat caaatgcgcc aaactgttga ggaaaagcta gaaaaaacgc tacaaacgcg
    15361 tttgcaaact tcttttgaaa ctgtatcgcg tcaactagag agtgtcaatc aaggtcttgg
    15421 ggagatgaaa actgttgccc aagatgttgg tacgctgaac aaagtcttgt caaatactaa
    15481 gacaagggga atattagggg agttacaact cggccaaatt atagaagata ttatgacagt
    15541 tagtcaatat gagagagaat ttcctacggt gtctggctct tctgagcgtg ttgaatatgc
    15601 tattaaatta cctggaaatg gtcagggaga ttatatctat ttgcctattg actctaagtt
    15661 tcctctagaa gattattacc gattggaaga tgcttatgaa ttgggtgaca aggttcaaat
    15721 agaactctat cgtaaatctt tactggcaag tattcgtaaa tttgcaaaag atataaacaa
    15781 taagtactta aatcctcctg aaacgacaaa ttttggtatc atgttcttac caactgaagg
    15841 gctctattct gaagtggtaa gaaatgcaac attctttgat agtctaagac gtgacgaaaa
    15901 tattgtagta gctggaccgt caaccttatc tgctttacta aattcattat ctgttggctt
    15961 taaaacctta aatattcaaa agaatgctaa tgatattagt aaaattttag ggaatgtcaa
    16021 ggttgaattt ggaaaatttg gtggcatgtt atcaaaagct caaaaacaat taaatacagc
    16081 aagtaaaagt attgataatt tactcacaac gcggacaaac gctattatca gagccttaaa
    16141 tacagttgaa gaacatcaag atcaagcaac cactagcttg ttaaaccttc ctataacaga
    16201 agaagaggaa attaatgaaa attaaccaaa tgaaaaaaga cgagcttttt gaggggtttt
    16261 accttataaa gaaagcagaa gttcgtaaaa cgcgtgcagg taaagacttt attgccttta
    16321 cctttcaaga tgatactggc gaaatttcag gaaatatgtg ggatgcacaa acctataatg
    16381 tagaagagtt tgtagccggt aaaattgttc atatgaaagg gcgtcgtgaa gtttataatg
    16441 gaactcccca agtcaaccaa attacattaa gaaacatcaa agatggtgaa cctaatgatc
    16501 ctagagattt taaagaaaaa cctccaatca atgttgacaa cgtgagagag tacatggaac
    16561 aaatgctctt taaaattgaa aatgcaacct ggcagcgtgt ggtaagagca ctttatcgta
    16621 aatacaataa ggaatttttt acctatccag ccgccaaaac aaaccaccac gcctttgaat
    16681 caggattggc atatcacacg gcaacgatgg ttcgtttggc agatagtatt ggagatatct
    16741 atccagaact taataaaagt ttgatgtttg ctggtattat gctacatgat ttagccaagg
    16801 tcattgagtt atcgggtcct gataatacag aatatactat tcgaggtaac cttatcggtc
    16861 atatttcact tattgatgag gaattaacaa aaatactagc agagcttaat attgatgata
    16921 caaaagaaga ggttacagtg ttacgccatg ttattctgag tcaccatgga caattagaat
    16981 atggaagccc ggttcgtcct agaatcatgg aggcagaaat tatccatatg atcgataaca
    17041 ttgatgcaaa tatgatgatg atgacaacag ctttaaatcg tgtcaacgaa ggtgaaatga
    17101 caaatcgcat ttttgccatg gataaccgtt ctttttataa actgaacatt aaataattta
    17161 ggtagaataa ttaagaccta aaataaaatg ttcgttttta gcatttgtta tgttatacta
    17221 tatgtaaaga atgctatgaa taaggagaaa atatgaaatt aagacgtagc gaacgcatgg
    17281 ttgttatttc aaattaccta ataaataatc cctatacatt aactagttta aatacatttg
    17341 ctagtaaata cggtgccgcg aaatcttcga tttcagaaga tattgctatt atcaaaaaag
    17401 cttttgaaca agctcaaatc ggagatatta aaactgtgac tggtgcaagt ggaggtgtta
    17461 tctttacacc aactattgct gaagcagaag cgaaagaaat cgttgaagaa ttaagacagc
    17521 gtttatctga aaacgatcgt attcttccgg gaggttacat ttatctttca gatctactca
    17581 gtacaccaaa aatgttacaa agtattggtc gcattgtagc gaacgcttac agaggtcaaa
    17641 aaattgatgc tgtcatgacg gttgcaacaa aaggtgttcc tttagcaaac gcagtagcaa
    17701 atgttttaga tgttcctttt gttattgtga gacgtgattt gaagattacg gaaggttcta
    17761 ctgtgtcagt aaactacgct agtggttcaa gtggccgtat tgaaaaaatg tttctttcta
    17821 agcgtagctt aaaacctaac agtcgagttt taatagttga tgatttcttg aaaggtggag
    17881 gtactgtttc ggggatgatt agtttactct cagaattcga tagcacatta gtaggtgtcg
    17941 ctgtatttgc agaaaatgct caggaacaac gtgagaagat ggcctataaa tctcttctga
    18001 gagtatctga aattgatgta aaaaataata gagtctctgt agaagcgggg aatatatttg
    18061 attaaagagt caaggtgaca tccttgattc tttttgcttg taaaagtaat aaaagcttga
    18121 cacatcatgt gtaaaatgat actatgataa acggtacttt tacttttggt ctctcaaaag
    18181 tgtacagaga cgtgctgaca attgttgcaa aagtacacgc agatataggc tgtcaccaat
    18241 tgctatatca accaaaaata aaaaacttac aggagaaatg tagatgccta caattaacca
    18301 gttggtacgt aaaccacgta aatctaaagt tgaaaaatca gattcacctg ctttgaatat
    18361 tggttacaac agtcacagaa aagttcacac taaattatct gcaccacaaa aacgtggtgt
    18421 tgcaactcgt gttggaacta tgacacctaa aaaacctaac tctgcccttc gtaaatttgc
    18481 tcgtgtacgt ttgagcaacc ttattgaagt tactgcttac attccaggta tcggacacaa
    18541 cttgcaagaa cacagtgttg tgcttatccg tggtggacgt gtaaaagacc ttccaggggt
    18601 acgttaccat atcgttcgtg gtgcacttga tactgcaggt gtagctgatc gtaaacaagg
    18661 ccgttctaaa tacggtgcta aacgtcctaa agggtaatta gaagggggat aagagaaaat
    18721 gagtcgtaaa aatcaagcgc ctaaacgcga agtattacca gatccattgt ataactcaaa
    18781 aatcgttaca cgtcttatca accgtgtaat gcttgatggt aaacgtggta ctgctgcaac
    18841 tatcgtatat gatgcttttg aagcaattaa agaagctact ggaacagatg ctcttgaagt
    18901 attcgaaaca gctatggaaa acatcatgcc tgtacttgag gtacgcgcac gccgtgttgg
    18961 tggatcaaac taccaagttc ctgtcgaagt tcgtccagaa cgtcgtacaa ctttaggact
    19021 tcgttggttg gttaacgcat cacgtgctcg tggtgaacac actatgaaag atcgtcttgc
    19081 aaaagaaatc atggatgctg caaacaacac aggagcatca gttaagaaac gtgaagatac
    19141 tcacaaaatg gctgaagcta accgtgcctt tgcacacttc cgttggtaag attactctct
    19201 ttttgagact tatcatagct actaaacaaa ttgtttggta gatagcaatg ttagcaatta
    19261 gaacgggtag gtcctgccta tccgtttttt ataaatagtg ttataataaa gaagtaaata
    19321 atttaaaata taggagaaac caaatggctc gcgaattttc acttgaaaaa actcgtaata
    19381 tcggtatcat ggctcacgtc gatgctggta aaacaacaac aactgagcgt attctttact
    19441 atactggtaa aatccacaaa atcggtgaaa cacacgaagg tgcttcacaa atggactgga
    19501 tggaacaaga acaagaacgt ggtatcacaa ttacatcagc tgcaacaaca gcacaatggg
    19561 atggtcaccg tgttaacatc atcgatacac caggacacgt ggacttcaca attgaagttc
    19621 aacgttcact tcgcgtactt gatggtgcgg taacagttct tgatgctcaa tcaggtgtag
    19681 aacctcaaac tgaaacagtt tggcgtcaag caacagaata cggagttcca cgtatcgttt
    19741 ttgctaataa aatggataaa atcggtgctg acttcctcta ttcagtacaa tcacttcatg
    19801 accgtcttca agcgaatgca cacccaattc aattgccaat tggttcagaa gacgatttcc
    19861 gtggtattat tgacttaatc aaaatgaaag ctgaaatcta tactaatgac cttggtacag
    19921 atatccttga agaagatatt ccagcagagt atgttgatca agcaaatgaa taccgtgaaa
    19981 aattagtaga agcagttgct gatactgatg aagatttgat gatgaaatac cttgaaggtg
    20041 aagaaatcac taacgaagag ttgatggctg ctattcgtag agcaactatc aatgttgaat
    20101 tctaccctgt attgtgtggt tcagccttca agaataaagg tgttcaattg atgcttgatg
    20161 ctgttattga ttaccttcca agcccacttg atattcctgc aatcaaaggt attaaccctg
    20221 atactgacga agaagaaaca cgtcctgctt cagatgaaga gccatttgca gcgcttgcct
    20281 tcaagatcat gacagatcct ttcgtgggtc gcttaacatt cttccgtgtg tactcaggtg
    20341 ttcttaactc aggttcatac gtgttgaata catctaaagg taaacgtgaa cgtatcggac
    20401 gtatcttaca aatgcacgca aatagtcgtc aagaaattga aactgtttat gctggggata
    20461 tcgcagcagc tgttggtttg aaagatacaa ctacaggtga ttcattgact gatgaaaaat
    20521 caaaagttat tcttgaatca attgaagttc cagaaccagt tatccaattg atggttgagc
    20581 caaaatctaa agctgaccaa gataaaatgg gtattgccct tcaaaaactt gctgaagaag
    20641 acccaacgtt ccgcgttgaa actaacgttg aaactggtga aacagttatc tcaggaatgg
    20701 gtgaacttca ccttgatgtc cttgttgacc gtatgaaacg tgaattcaaa gttgaagcaa
    20761 acgttggtgc acctcaagta tcataccgcg aaacattccg cgcttctaca caagcacgtg
    20821 gattcttcaa acgccaatct ggtggtaaag gtcaatttgg tgatgtttgg attgaattta
    20881 caccaaacga agaaggtaaa ggattcgaat tcgaaaatgc tatcgtcggt ggtgtggttc
    20941 ctcgtgaatt tattccagcc gttgaaaaag gacttgtaga atctatggca aacggtgttc
    21001 ttgctggata tccaatggtt gacgtaaaag ctaaacttta cgatggttca tatcatgatg
    21061 ttgactcatc tgagacagcc ttcaagattg ctgcatcact tgcacttaaa gaagctgcta
    21121 aatcagcgca accagctatc cttgagccaa tgatgcttgt tacaattaca gctccagaag
    21181 acaatcttgg tgacgttatg ggtcacgtga ctgcacgtcg cggtcgtgtt gatggtatgg
    21241 aagcacgtgg taacacacaa gttgttcgtg cctttgttcc acttgctgaa atgtttggtt
    21301 atgcaacagt tcttcgttct gcaacacaag gacgtggtac attcatgatg gtatttgacc
    21361 actacgaaga tgttcctaag tcagttcaag aagaaatcat caagaaaaac tctggtgaat
    21421 aatttctgaa aacttatgaa aggtactcga aagagtatct ttttttcttt ttgaaagtgg
    21481 taaattcatt gcaatttttc tcgaaaaaag atataataat tgtgttgaaa gatttttgca
    21541 catatgtaca agttaatctt ttcacattgg ttagggggat ccctaaaata aaatatttga
    21601 gattttcata aggaggaaat cactaatggt agttaaagtt ggtattaacg gtttcggtcg
    21661 tatcggtcgt cttgcattcc gtcgcatcca aaacgtagaa ggtgttgaag ttactcgtat
    21721 caacgacctt acagatccaa acatgcttgc acacttgttg aaatatgaca caactcaagg
    21781 tcgtttcgac ggtactgttg aagttaaaga aggtggattc gaagttaacg gtcaatttgt
    21841 taaagtttct gctgaacgcg aaccagcaaa cattgactgg gctactgatg gcgtagaaat
    21901 cgttcttgaa gcaactggtt tctttgcatc aaaagaaaaa gctgaacaac acatccatga
    21961 aaatggtgct aaaaaagttg ttatcacagc tcctggtgga aacgacgtta aaacagttgt
    22021 tttcaacact aaccacgata tccttgatgg aactgaaaca gttatctcag gtgcttcatg
    22081 tactacaaac tgtcttgctc caatggctaa agctttacaa gacaactttg gtgttaaaca
    22141 aggtttgatg actactatcc acgcatacac tggtgaccaa atgatccttg acggaccaca
    22201 ccgtggtggt gaccttcgtc gtgctcgtgc aggtgctgca aacatcgttc ctaactcaac
    22261 tggtgctgca aaagctatcg gacttgttat cccagaattg aacggtaaac ttgatggtgc
    22321 tgcacaacgt gttcctgttc caactggatc agtaactgaa ttggttgcaa ctcttgaaaa
    22381 agacgtaact gttgaagaag taaatgcagc tatgaaagca gcagctaacg attcatacgg
    22441 ttatactgaa gatccaatcg tatcatctga tatcgttggt atttcatacg gttcattgtt
    22501 tgatgctact caaactaaag ttcaaactgt tgacggtaac caattggtta aagttgtttc
    22561 atggtacgat aacgaaatgt catacacttc acaacttgtt cgtacacttg aatactttgc
    22621 aaaaattgca aaataatttt taattaatat ga
//
LOCUS       DBJYSH010000016        22441 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.016, whole genome shotgun sequence.
ACCESSION   DBJYSH010000016 DBJYSH010000000
VERSION     DBJYSH010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 22441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22441
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.016"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            48..1070
                     /gene="add"
                     /locus_tag="ACUBV3_000510"
     CDS             48..1070
                     /gene="add"
                     /locus_tag="ACUBV3_000510"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HHU3847042.1"
                     /translation="MTQAVLKELAKAELHCHLDGSLSLPAIRKLANMADIILPSSDKE
                     LRKYVIAPAQTESLVDYLKTFEFIRPLLQTKEALRFAAYDVARQAALENVIYIEIRFA
                     PELSMDKGLTASDTVLAVLEGLADAQKEFNIVARALVCGMRQSSHKTTKDIIKHIVDL
                     APKGLVGFDFAGDEFSYPTDSLVDLIQEVKRSGYPMTLHAGECGCAKHIADSLNLGIK
                     RMGHVTALTGQRDLIKRFVEEDAVAEMCLTSNLQTKAASSIQSFPYQELYDAGGKITI
                     NTDNRTVSDTNLTKEYSLFVTYFGTKIEDFLVFNQNAVKASFTSDSEKDTLLHKLQEN
                     YDSYLK"
     gene            1129..1572
                     /locus_tag="ACUBV3_000511"
     CDS             1129..1572
                     /locus_tag="ACUBV3_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HHU3847043.1"
                     /translation="MALAKIVYASMTGNTEEIADIVADKLRDLGLDVEVEECTMVDAA
                     DFEDADIAIVATYTYGDGDLPDEIVDFYEDLAEVDLSGKVYGVVGSGDTFYDYFCKSV
                     DEFEAQFALTGAQKGADCVKVDLAAEDEDIENLEAFAEEIASKLN"
     gene            1649..1924
                     /locus_tag="ACUBV3_000512"
     CDS             1649..1924
                     /locus_tag="ACUBV3_000512"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HHU3847044.1"
                     /translation="MELEEIRQEIDEIDQQLVSLLETRMGLILEVIAFKKKHRLPVLD
                     NNRENEVLNNVLKKVQNHQFDDVIRATFKDIMTESRVYQKENIVDGD"
     gene            1917..3113
                     /locus_tag="ACUBV3_000513"
     CDS             1917..3113
                     /locus_tag="ACUBV3_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HHU3847045.1"
                     /translation="MIKRFDNYAQYVLVLGFSLLIGGVVGAITAVFGRVLLFLTAFRS
                     DYIAYLLPFLSIVGLFIVFVYQKFGGKSVKGMGLVFEVGHGNEETIPKRLVPLVILTT
                     WLTHLFGGSAGREGVAVQIGATVSHYFQKYCRLQNASQLFLVMGMAAGFAGLFQTPLA
                     ATFFAIEVLVVGRLMVSYILPSLIAALTANFVSHSLGLEKFSHSIATSMALTPDIILK
                     LLVLGLCFGLCGNLFAYLLAKAKLIASSRLLNPYKRIFTLGLLATFLLFIFHFGRYSG
                     LGTNLIEASFTNKNLYDYDWLLKLCLTVITLAAGYQGGEVTPLFAIGASLGVIIAPIL
                     GLPVILVAALGYTSVFGSATNTLLGPILIGGEVFGFANTPYFVIVCLVAYSISHAHTI
                     YGAQSR"
     gene            3697..4044
                     /gene="rplS"
                     /locus_tag="ACUBV3_000514"
     CDS             3697..4044
                     /gene="rplS"
                     /locus_tag="ACUBV3_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HHU3847046.1"
                     /translation="MNPLIQSLTEGQLRSDIPEFRAGDTVRVHAKVVEGTRERIQIFE
                     GVVISRKGQGISEMYTVRKISGGIGVERTFPIHTPRVDKIEVVRYGKVRRAKLYYLRA
                     LQGKAARIKEIRR"
     gene            4155..4226
                     /locus_tag="ACUBV3_000515"
     tRNA            4155..4226
                     /locus_tag="ACUBV3_000515"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4188..4190,aa:Arg,seq:cct)
     gene            complement(4269..5347)
                     /locus_tag="ACUBV3_000516"
                     /pseudo
     CDS             complement(4269..5347)
                     /locus_tag="ACUBV3_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000264737.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(5471..6364)
                     /locus_tag="ACUBV3_000517"
     CDS             complement(5471..6364)
                     /locus_tag="ACUBV3_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P63C domain-containing protein"
                     /protein_id="HHU3847047.1"
                     /translation="MENEVLHATHEGKLVLDNTTIDVAVLNNGQRIITQAGVFKALDR
                     PSRGNTRVIGIPTFMDAKNLQPLISKDLRGVINKIEYRDLNGRIQLGFDANILPLVSD
                     LYLKAREIGAIKSEAQMRTAQKAEMLVRSLAKVGVTALVDEATGYQYERERFELQKIF
                     KAYISEELLKWQKMFPDDFYMEIFRLNGWSYDVQSIKRRPGVVGKWTNELIYKRLPDG
                     VLEELKRRTPKTKKGQYKARFFQTLTPDIGHPDLNAQIYKVLGIMRISSNWKDFKEKF
                     NVMTSREDGIIELDLEFDEDK"
     gene            complement(6536..7093)
                     /locus_tag="ACUBV3_000518"
     CDS             complement(6536..7093)
                     /locus_tag="ACUBV3_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HHU3847048.1"
                     /translation="MLEKVERLISEINRIHLVYSQDYFETGKVEKINLKHTFSKVPVK
                     AILDYRLNLHESINDYLMKADVKDIPYVYRVKTSESILDKIERFSKRQDGYPVNSILN
                     DIFGARIILSSEDISQVMEQLDEWKDKFDLKNWYLRDKDNYTGIHVYFKNKSNHYYPW
                     ELQIWDEKDVDQNIESHKLFKRHFV"
     gene            complement(7095..7835)
                     /locus_tag="ACUBV3_000519"
     CDS             complement(7095..7835)
                     /locus_tag="ACUBV3_000519"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000016871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HHU3847049.1"
                     /translation="MSGSLGNKKIMASNIRRHLEKKNMNPKEFSKVMNFKYSTVLDWV
                     NAKTYPRIDKIELMANYFNVEKSDLVEVYSHDNISALSNIVIELDKGLKEPRHSDWIS
                     YGENLLSEQNTVEDSKNTVVELFSYNYYDHAASAGTGQYLNDVQVETIELPVDYDADF
                     VIPVYGDSMEPDYHSGDYVFVKLSVELVDGDIGVFEYYGDAYIKQLLIHAEGAFLHSL
                     NDKYNDILIDRDSDFRIIGKVVGSYRES"
     gene            8198..8341
                     /locus_tag="ACUBV3_000520"
     CDS             8198..8341
                     /locus_tag="ACUBV3_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3847050.1"
                     /translation="MEESILNSKRYTKLVVETEDGKKIAEVTLTEATPADGYVIRLTP
                     NYD"
     gene            complement(8338..8562)
                     /locus_tag="ACUBV3_000521"
     CDS             complement(8338..8562)
                     /locus_tag="ACUBV3_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001107539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HHU3847051.1"
                     /translation="MAKNQHVVPNPNGGWDIKGAGNSRATKHTTTQAEAVEIATGIAK
                     NNSSEVIIHGKNGRIRERNSYGNDPFPPKG"
     gene            8621..8779
                     /locus_tag="ACUBV3_000522"
     CDS             8621..8779
                     /locus_tag="ACUBV3_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3847052.1"
                     /translation="MNWKKLMFGDLEHTFTSRNGKEKTSIEFEGGVLPALLVLGGITW
                     LIAWFITK"
     gene            complement(8926..9621)
                     /locus_tag="ACUBV3_000523"
     CDS             complement(8926..9621)
                     /locus_tag="ACUBV3_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3847053.1"
                     /translation="MSKKSCFVVSAIGEESSEIRNHSDSVLNYIIKPALIEKYQVTRA
                     DELYHSDRIDDKIFDALSTADLVIVDITGNNPNVFLELGFRKALNLPTIFLRQKTDED
                     IPFDIRTINTIHYDLKNSESKVVLDSVQETIRRIQKTEENIDFSIIHEPNDQSASVQD
                     IIQLKTSINNIYDAIENLSDKIENNSTQKRPMTQEDLIMMAFQEPEKLEKIFELQTKY
                     PNAFNSPSNALND"
     gene            9676..9885
                     /locus_tag="ACUBV3_000524"
     CDS             9676..9885
                     /locus_tag="ACUBV3_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3847054.1"
                     /translation="MRPKKYPYSQKNYPAPKLHNIIETDNHFLIDDKRIHYVIEDSVK
                     TKTLGDGYVEVTLSIIAKSFTKSQG"
     gene            complement(9874..10260)
                     /locus_tag="ACUBV3_000525"
     CDS             complement(9874..10260)
                     /locus_tag="ACUBV3_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3847055.1"
                     /translation="MFDYFKLYTIVLETFVETKPDDAYSLFDNLSLNSEFVKFSKDLD
                     KNIVISGTLEVIDNLLDDCLIKGKRRPTKDITFYFFDGVTTTGYLYLQSLKDNNFSSR
                     LKNILKEEGIPLTPTSITRTIAKLTL"
     gene            10334..10540
                     /locus_tag="ACUBV3_000526"
     CDS             10334..10540
                     /locus_tag="ACUBV3_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006735559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HHU3847056.1"
                     /translation="MTKMTLKMLRVSKNWNQETAAKKLKISVSKLSNWENAKTFPDAI
                     EINKIEKLYDVNYSDIIFYQQNTV"
     gene            10582..11502
                     /locus_tag="ACUBV3_000527"
     CDS             10582..11502
                     /locus_tag="ACUBV3_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001006816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3102 domain-containing protein"
                     /protein_id="HHU3847057.1"
                     /translation="MNEVTLSNNLQQIELDLRQENEQIGKSIWKIGCMLKHVKENDLT
                     HGQFMDWYKNLGYNKNFVSKAITIADKLSNFPTLGNIGTEALYLIATLPDEQKQEQIE
                     WIESGDNPTVRELQEIKRENNRLKSENARLEQQKENLAEQALSAKIVEKEVIKEVIPD
                     DYESTKQLNQTLLGKNKELSKMVDDALQHEEYLKSQLKEFYAKRDEVNHKSAKYDELT
                     EAIKQSEGKLNSYQKKIASYKNITELLKKGDLLLLEMSGLIYADETHYIQRDGLIKQE
                     FDSLVDRGLKLFNDLDMKRKNTEILEGEVL"
     gene            11499..11954
                     /locus_tag="ACUBV3_000528"
     CDS             11499..11954
                     /locus_tag="ACUBV3_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001872799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6C domain-containing protein"
                     /protein_id="HHU3847058.1"
                     /translation="MNELTTTQQLIEMSKMQTVTLEKVDNLEKGLLQAQNDIQEIMDT
                     SYLHPGIINMITKKRRKRVIDCMGGKSAKAYKTFKVDEEGRKHRFSSEVFREMELDFK
                     AEFDLNSYAELSKSKKEEALEYIAMWEPCTNTKRKINLLNKQTELELIG"
     gene            11987..12145
                     /locus_tag="ACUBV3_000529"
     CDS             11987..12145
                     /locus_tag="ACUBV3_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3847059.1"
                     /translation="MEEDIKQLITENILEFLGSDYGKGFLTGIKIAVEIIAKQIPTPS
                     EEDIGHDS"
     gene            12272..12418
                     /locus_tag="ACUBV3_000530"
     CDS             12272..12418
                     /locus_tag="ACUBV3_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001867241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3847060.1"
                     /translation="MITILKEINQTLKEILAELKEPTVVTVDSEKLSNTLTTEQKIRR
                     LSGQ"
     gene            complement(12381..12689)
                     /locus_tag="ACUBV3_000531"
     CDS             complement(12381..12689)
                     /locus_tag="ACUBV3_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001000651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3847061.1"
                     /translation="MNDVLKTNLIADVAIFSEKSNCKLNVITASGIFTGTLLPENPDK
                     AKYAHVLEFLEYRKENKDDNERYMLLVDATLSTSKESTLNLPFVVLFIDQIIGVSSVQ
                     "
     gene            12795..13025
                     /locus_tag="ACUBV3_000532"
     CDS             12795..13025
                     /locus_tag="ACUBV3_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3847062.1"
                     /translation="MLAKLKSGIEVPYEELWLNDNDLSEFIGKSFDQTQRLLRKMYKD
                     RNYRKYIDKVGGRSTKVKKFEEWRKLQNEKII"
     gene            13009..13155
                     /locus_tag="ACUBV3_000533"
     CDS             13009..13155
                     /locus_tag="ACUBV3_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3847063.1"
                     /translation="MKKLFNFIFTKPKKEEPKPKRTIETHGWEVGAKAYDDFHNYMKG
                     LKHG"
     gene            complement(13433..13525)
                     /locus_tag="ACUBV3_000534"
     CDS             complement(13433..13525)
                     /locus_tag="ACUBV3_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HHU3847064.1"
                     /translation="MCEIFFTTIIAPLLVGIVLLLVQKWLDNDD"
     gene            14090..14260
                     /locus_tag="ACUBV3_000535"
     CDS             14090..14260
                     /locus_tag="ACUBV3_000535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3847065.1"
                     /translation="MAKMGRPTSNPRDKYIGVRASKNEVEMLDFCTEKTGKSKTDILM
                     EGLEKVYNELKG"
     gene            14437..14877
                     /locus_tag="ACUBV3_000536"
     CDS             14437..14877
                     /locus_tag="ACUBV3_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3847066.1"
                     /translation="MKTYTLTEEELNELVAERMKQAKEKRTPQGLFKDVGFDDELIPI
                     NNKYPKVLKKLNRERAYKPEKHVFNQTPKVFGVDNEISYSKITTHDVHNHIRLLVLNV
                     FGKSQNKEVLPEEYDQAIELYNQLKEWFVSSYDKRLEGLVLEDD"
     gene            14994..15908
                     /locus_tag="ACUBV3_000537"
     CDS             14994..15908
                     /locus_tag="ACUBV3_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000514063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HHU3847067.1"
                     /translation="MGKEKTKIYFWLKFDKKFFENIFIKRLKKMAGGDTMTVLYIRLM
                     LESLETDCILYYEGYFDNLVEELAIKLEVSEDDINMTLAYFTKCGLIQIDGSGNAQMP
                     QAKAMLESETNWAKYKRETRKIGQIPTDVQPMSNQCPTEIEIDIEKKKDKERELDKEK
                     EYIVEQSPTEYLFPDWLEEKYVEQVKKGNPKNFDYRIPIAYLNQKMNSNYKFVKTNTD
                     LVKARLKDSYTLEDFKAVIDKKCSEWVNSDMEKYLRPSTLFNASKFESYLNQPEVAKS
                     DYYQKQQGQRFSQAELDELKKPDPKYGF"
     gene            15918..16760
                     /locus_tag="ACUBV3_000538"
     CDS             15918..16760
                     /locus_tag="ACUBV3_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HHU3847068.1"
                     /translation="MAFGLMTRESMLENGIIRDTGKTCEKHEMPIYARKMPNHGNRET
                     EFCWQCTTEYIQTKSNAVDIAYNNQSLLAKGYKVFYKESVLSKEIASATLKNYKEHSA
                     VDTKALNYAKRITRDYVKGMEGNSLLQGPPGVGKSHLSMSIAKNINEMFKSYNQSKSV
                     IFVSVPLLSGLVKDTFDYDDKKNSKYSQERMSKLLINCDYLILDDLGKESTTGNTIKS
                     ASGWTYTFLFNILDNRTNTIINTNFSRAELMKIYDAAFVDRIIKGAKNNIFKYPDNAE
                     SKRF"
     gene            16760..16906
                     /locus_tag="ACUBV3_000539"
     CDS             16760..16906
                     /locus_tag="ACUBV3_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3847069.1"
                     /translation="MELTLTTFFSLSEEHAAKIMALDEPSRNKKIEEYRQLRLRRGRI
                     DFGK"
     gene            16896..17171
                     /locus_tag="ACUBV3_000540"
     CDS             16896..17171
                     /locus_tag="ACUBV3_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000431574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3847070.1"
                     /translation="MENRPDLKLVAELENRIKDLKIENEILKSKNIDLSEDVKHLKYK
                     CFEKDSSIVDIMVNNNDLRKENNELREMFDFFKDRLEKFVGSSHGRN"
     gene            17158..17412
                     /locus_tag="ACUBV3_000541"
     CDS             17158..17412
                     /locus_tag="ACUBV3_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008088460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3847071.1"
                     /translation="MVEIRINGELVTFDGNFRDAFIFTIDYLRDSEEPTLRQTYNEFK
                     DYTDEDLMEYIETEFDVKPELIVNRRLDSKWAFKSHILED"
     gene            17415..17576
                     /locus_tag="ACUBV3_000542"
     CDS             17415..17576
                     /locus_tag="ACUBV3_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3847072.1"
                     /translation="MSEELYESTRYWQSRYSDLMSDYLKEAEENIELKKQLKRLKAEN
                     WQLKHRKRK"
     gene            17577..18059
                     /locus_tag="ACUBV3_000543"
     CDS             17577..18059
                     /locus_tag="ACUBV3_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HHU3847073.1"
                     /translation="MAYLYELQGIYAQLSAMDLDDETFQDTLDSIDFQSDLENNIEYF
                     VKMLKNTQADIEMYKNEKETFYKKQKQAEAKAEKYKDTIRLAMDLSQKKKVDAGMFKV
                     SLRKSKKVEVLDETKIPFEYMQEKVEYKPKKDEISKVLKSGIDISGVQLIETESLQVK
                     "
     gene            18060..18734
                     /locus_tag="ACUBV3_000544"
     CDS             18060..18734
                     /locus_tag="ACUBV3_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
                     /protein_id="HHU3847074.1"
                     /translation="MSMTFAELQTKMQITKTTKQGVKYTFRNAEDIFTHFKTLNSGWE
                     LTVSDELVELIGRIFIKATATARLGDEQHQATRCAELDSVPVLNTKDYKTGEPKQIQQ
                     MQVPQWTGAVSSYAGKYALQGLFGIGEEDVDAIVTEDTQRKEQKTSRPTTSKTPKISN
                     IQVETYKSDLNDIAKATNQNVEELTKWLTDTLKVGTLENLHTEHIVSADELINKLKKK
                     AGLKND"
     gene            18727..19143
                     /gene="ssb"
                     /locus_tag="ACUBV3_000545"
     CDS             18727..19143
                     /gene="ssb"
                     /locus_tag="ACUBV3_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HHU3847075.1"
                     /translation="MINNIVLVGRMTKDAELRHTPSQVAVATFTLAVNRRFKEQNGER
                     ETDFINCVIWRQSAENLANWAKKGTLIGITGHIQTRNYENQQGQRIYVTEVVAENFQL
                     LESRNSQQQTNQSGNSSNSHFGNANKMDISDDDLPF"
     gene            19146..19346
                     /locus_tag="ACUBV3_000546"
     CDS             19146..19346
                     /locus_tag="ACUBV3_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3847076.1"
                     /translation="MTKPRKQRIYAIYDDDKFVDVGTKEELSARLGIKKATIEQYMTK
                     SYQARPSSKRIAIFVGVEEIEF"
     gene            19336..19824
                     /locus_tag="ACUBV3_000547"
     CDS             19336..19824
                     /locus_tag="ACUBV3_000547"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001009815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0000400 - four-way junction DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HHU3847077.1"
                     /translation="MNFKTEFEIPVEPKPQTRPKFSKWGTYEDPKMKKWRKQVTGWIE
                     KNYNGPFFDNCVKVDVTFYMRAPQTLIKEPTARSKSKTVQIYQKFINELIWHVKKPDI
                     DNLVKAVFDSISDAGYDKIQKSGIVWSDDNIVCDLRATKKYSPNPRIKVKIEEIDERT
                     NG"
     gene            19805..20149
                     /locus_tag="ACUBV3_000548"
     CDS             19805..20149
                     /locus_tag="ACUBV3_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001004391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3847078.1"
                     /translation="MNELTDKFYSIFDSSILRRVKELNLDDKTSERLRLNISNNKRRN
                     ILPRPYVIEAFKDYFDKDTYVQLYLKSYREYHNPNSHETDIFIKLNKKHRDTKLDHYK
                     KVKRLMYAAMTF"
     gene            20161..20457
                     /locus_tag="ACUBV3_000549"
     CDS             20161..20457
                     /locus_tag="ACUBV3_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide modification associated
                     domain-containing protein"
                     /protein_id="HHU3847079.1"
                     /translation="MADKINAESMQAAYNENYQMFLAKNADYGNSFEKSLNDFGFIAG
                     VVRIGDKYNRLYNLISSDKNVSESLSDTLNDMANYCTMLSIWLEKTENANDTRS"
     gene            20441..20680
                     /locus_tag="ACUBV3_000550"
     CDS             20441..20680
                     /locus_tag="ACUBV3_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000150115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3847080.1"
                     /translation="MTHVVRVYDHIGGRVLPITRRVRRMTKYIEIKDDWKKAVDHLND
                     FIEKYSYKKIVVVGYQVVRNPETNGTCTHVLVKVD"
     gene            20711..20875
                     /locus_tag="ACUBV3_000551"
     CDS             20711..20875
                     /locus_tag="ACUBV3_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3847081.1"
                     /translation="MTKEEVIAFLTEQRDLRLVAYEWGKDNLSVFARWQLEQANMYLD
                     IIEWIEEVTE"
     gene            20872..21141
                     /locus_tag="ACUBV3_000552"
     CDS             20872..21141
                     /locus_tag="ACUBV3_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3847082.1"
                     /translation="MTEQQMIDCLLYELAKKDKLNIRRNNIITFLSIVLMAISILNVA
                     LQDHYKLQITGLRTQLSRTQKQLKRASEQNQRQTKRIAELTGNGG"
     gene            21145..21288
                     /locus_tag="ACUBV3_000553"
     CDS             21145..21288
                     /locus_tag="ACUBV3_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3847083.1"
                     /translation="MIDEILKRLNKEFDNDLDNYEQERYAGYMDAIGVAIEIVEEVKR
                     GRK"
     gene            21285..21797
                     /locus_tag="ACUBV3_000554"
     CDS             21285..21797
                     /locus_tag="ACUBV3_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HHU3847084.1"
                     /translation="MNIEEAKKLIDKQSIGKGGVGDIPVVKTHIVKVLLDQLDKPKLE
                     VPQMIADVIESFDEDVNYLHEHMSYQSDEVREWLTHNEREFYEAWLAYPNITVEKENL
                     YTVEIPNPNERQLSFVLMRQLSGNVSIKVMHRDNLDLLKIDNNLQLTESEIRKDFDWA
                     WQFRKDVENE"
     gene            21818..22117
                     /locus_tag="ACUBV3_000555"
     CDS             21818..22117
                     /locus_tag="ACUBV3_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3847085.1"
                     /translation="MNYDKNKNDAKKNFIIALVLLPFGLVLSGFVIKYGWNNILSTID
                     GVPSINLPQAVGINVLISPFASKKNTDEDFATVIARAFISPLVVLLLLWIVTLFM"
BASE COUNT         7853 a         3545 c         4326 g         6717 t
ORIGIN      
        1 taacttttgt taatatagta aaaatgcttt ttaataggag ataaattatg acacaagcag
       61 tattaaaaga attggctaag gcggagttgc attgtcatct agatggctca ctttctttac
      121 cagctattag aaaattggct aatatggctg atataatact gccgagtagt gataaagaat
      181 taagaaaata tgttatagct ccagctcaaa cagaaagctt agtagattac cttaaaacgt
      241 ttgagtttat cagaccgctt cttcaaacga aggaagcatt acgttttgct gcttatgatg
      301 ttgctagaca ggcagcttta gaaaatgtta tttatattga gattcgtttt gcgccagaac
      361 tctctatgga taaaggttta actgcaagtg atacagtcct tgcagtctta gaaggtttag
      421 cagacgctca gaaagaattt aatatcgttg cacgcgctct tgtttgcggt atgcgccaat
      481 cgtctcataa aacgaccaag gacattatta aacacattgt tgatttggct cccaaaggcc
      541 tggtaggttt tgattttgca ggtgatgaat tttcctatcc aacagatagt ttagtagatc
      601 ttattcagga agtaaaacgc tcaggttacc cgatgacttt gcatgcaggt gagtgtggtt
      661 gtgctaagca tattgcggat agtttaaatt tagggattaa gcgtatggga catgtgaccg
      721 ctttaacagg acagagggat ttaataaagc gatttgtcga agaagatgct gtagcagaga
      781 tgtgtttaac tagcaattta caaacgaaag cagcctcaag tattcaaagt tttccttatc
      841 aagaacttta tgatgctgga ggtaagatta caattaatac agataatcgt actgtttccg
      901 atacaaattt gacgaaagaa tatagtcttt tcgtaactta ttttggaacg aaaatagaag
      961 actttcttgt ttttaatcaa aacgctgtga aagcctcctt tacaagtgat tcagagaaag
     1021 atactttact acataagcta caagaaaact atgacagcta tctaaaataa tcttcacgat
     1081 tctttaaaaa tatgatagaa tagtggtaat ttacaagtgg aggaagatat ggctttagca
     1141 aagattgttt atgctagcat gaccggaaat actgaagaaa ttgctgacat tgttgctgat
     1201 aaattacgtg acttgggtct agatgttgaa gtagaagagt gtacgatggt tgatgctgca
     1261 gattttgaag atgctgacat tgcaattgtt gccacttata catatggtga tggagattta
     1321 ccagatgaaa tcgtggactt ttatgaagat ttagcagaag ttgatttatc tggtaaagtt
     1381 tacggcgtag taggttcagg tgatactttc tatgattact tctgcaaatc tgttgatgag
     1441 ttcgaggctc aattcgcatt aacaggagca caaaaaggtg ctgattgtgt taaagttgat
     1501 ttagcggcag aagacgaaga cattgaaaac ttagaagctt ttgctgaaga aattgcttct
     1561 aaacttaact aagctaccaa aaagacgaac acgagtcgtc tttttaactt ttttaccatc
     1621 ttttgtaact agaatatgag atatagatat ggaattagaa gagattcgtc aagaaataga
     1681 tgaaatagat cagcaattgg taagcctttt agaaactaga atgggtctaa ttttagaggt
     1741 catagcattt aaaaagaaac accgacttcc agttttagat aataatcgtg aaaacgaagt
     1801 gcttaacaat gtattgaaga aagtccaaaa tcatcaattt gacgatgtga tacgagcaac
     1861 tttcaaagac attatgacag aatctcgtgt ttatcaaaag gagaatattg tagacggtga
     1921 ttaaacgatt tgataattat gctcagtacg tacttgtttt agggtttagc ttactaatcg
     1981 gtggtgtggt tggggctatt acagcagtct ttggacgtgt tctattattc ctgacagcct
     2041 ttagaagtga ttatatagct tacctactcc cttttcttag cattgttggt ttatttatag
     2101 tttttgtcta tcaaaaattt gggggtaaga gtgttaaagg gatgggattg gtatttgaag
     2161 taggtcatgg taatgaggag actattccga aacgtcttgt tcctctcgtg atattaacaa
     2221 cttggttaac ccacttattt ggtggtagtg caggtcgtga gggggttgct gtacagattg
     2281 gggctaccgt ttcacactac tttcaaaaat attgccgttt acaaaacgct tctcaacttt
     2341 tcctagtaat gggaatggca gcaggttttg ctggtttatt tcagactcct ttagctgcta
     2401 ctttttttgc gatcgaagtt ttggtagtag gtcgtttaat ggtttcgtat atattaccaa
     2461 gtttaattgc tgctctgaca gcaaatttcg tctcacattc tttaggttta gaaaaatttt
     2521 ctcatagtat agcaacatca atggcgttaa caccagatat tattctaaaa ttattggttt
     2581 taggactatg ttttggtctg tgtggcaatt tatttgctta tttattggct aaagctaaat
     2641 taattgcttc tagtaggtta ctaaacccat ataaacggat tttcacactt ggattgttag
     2701 ctactttttt attgtttatc ttccattttg gacgatattc aggactggga acaaatctaa
     2761 ttgaggctag cttcactaat aagaatcttt atgattatga ttggttgtta aaattgtgtt
     2821 tgacagttat tactttggct gcaggttatc agggaggaga agtaactccc ttatttgcaa
     2881 taggagcaag tttaggagtg attattgcgc ccattttagg tttgccagtt attttagttg
     2941 ctgctttagg ctatactagc gtttttggga gtgcaacgaa tactctgtta ggtcctatct
     3001 taataggtgg cgaagtcttt ggctttgcca acactcctta ttttgttatt gtatgtcttg
     3061 ttgcttactc catatcacat gctcatacga tttatggtgc tcaatctaga tagttagtga
     3121 tgagttttaa aagagtttat cagtttatag aacggaatat tccgttctct tttttattct
     3181 cttaaagttt ataaaaatga agtttgtaaa ttttatgaat gcaaaaatta tgtggctaat
     3241 ataattttct tcaaagaaat tgattttatg attgtcatta aaatactgta aaatccaatt
     3301 gaatgtgtaa gaacttaggt taggataatg tctacaagca tttaagtcgc gatggtgaaa
     3361 tgcattagaa gataaaaagt tgatgtggat ttcaacttta tgggaaagag tcaggtgaca
     3421 cgaaagctaa tatagaaaac gtagtttaaa aaatcatata atattaaatg aattaaactc
     3481 tcaaaaatca gaaaaaagct aacctagaaa cagtaaatca aatttcaata atatacaaat
     3541 aactattcta aaggatgaca ccttgttctt ctagataaaa tagctttaca agtaggttaa
     3601 aatttggtag aattgtgaga gtgtgtaatg gacgcacaaa aaacggctaa tccgctgaga
     3661 caagtactta agattagtaa gagaaggaga ataaaaatga atccattaat tcaaagtttg
     3721 acagaaggtc aacttcgttc tgatatccct gagttccgtg ctggtgatac tgtacgtgtt
     3781 cacgctaaag ttgttgaagg tactcgcgaa cgtattcaga tctttgaagg tgttgttatc
     3841 tcacgtaaag gtcaaggaat ctcagaaatg tacacagtac gtaaaatttc tggtggtatc
     3901 ggtgtagagc gtacattccc aattcacact cctcgtgttg ataaaatcga agttgttcgt
     3961 tatggtaaag tacgtcgtgc taaactttac tacttacgcg cattgcaagg taaagctgca
     4021 cgtattaaag aaatccgtcg ttaattttga tgatcagatt ttaaaaatgc ttggttgttt
     4081 gaggatagta actatgtttt aaaactggac aaccaagacg taaaaaatct gcctgtgggc
     4141 agttttttta ctaggtcccc ttagttcaat ggatataaca actccctcct aaggagtaat
     4201 tgctggttcg attccggcag gggacatgta aataacgtca aaagcctttg tattaaaggc
     4261 tttttgtttt atttcgattt taaaaggggc acaaaagggg cagtttgttt atttataatt
     4321 tctttcatat ttacagttgt gtgactgtaa atagataatg ttgtttttgg atcgctgtga
     4381 ccaactctat ccattatcgc atttagcggt atcccttttt ctgctaaaaa tgatatatgc
     4441 gagtgtctaa ataagtgcgt gtgataatct ccataaattt tcaatcgctt attgatgtac
     4501 gcgtttaaaa tcggtacacc gttcgaattt ggaaagacga atgaacttgt ctttttctgt
     4561 ctattgacga tatctaaaat attatctgat acagatattt tgcgtgttga ttttttggtc
     4621 ttggtcgtag tgatttctcg tgtgttaaaa tcgtaagttg cattaatcag aatttcttta
     4681 ttttcaaaat ctattttgtc gtaagtcaga caagctaatt ctccataccg tacacccgtc
     4741 aaaaacatga ataaaacgat gtctgcgagc gtttgttcgt cattgtctat cattcattgc
     4801 acaggtcgta aacctcgtta gatgttaaat aaagcacctt ttcaggcttg taatcatcct
     4861 ttggtttggg aactaagacg ttctccgtcg gattacttgt catatagtcc atttgtatcg
     4921 cataagaaaa tatagcatgc aatctctttc tacatttatg cgtaacatga tacgaattgt
     4981 gttttaatag tttatcaatg attagtctaa tatcacgttt agtcaacttg ttgataattg
     5041 tatcatctgg taacacggtt gctatatgac tgtcagatac taaataacca cgttttgtgg
     5101 agtctttgac ggtcggtatc cattgcttta gatattcctt tttaagttct ccgtaagtca
     5161 tttctgagtg atttctgata gcgagcctat cttcaatctt ttcctgcaat attaaaccag
     5221 cttttttctg agcttgacta gaatttttat ctagtgtcac agatactttt ttgtacttat
     5281 ttgttagagg gtcagtatat ctttcgatat atttaaattt cccgttggct agctcctcta
     5341 tccacattgt tttatacctc attttctgtt aaaataggta tggtaaaaac ccctcccaaa
     5401 aagcaggttt ttactatact agaatttgcc tcacgctctc cttggccaaa atttgagcgt
     5461 ggggcttttt ttatttgtct tcatcaaatt ctaaatctaa ttcaattata ccgtcttctc
     5521 tagatgtcat aacattaaat ttctctttga aatctttcca gttagatgaa attctcataa
     5581 tcccaagaac tttataaatt tgagcgttta aatcaggatg tcctatatct ggtgttaaag
     5641 tttggaaaaa tctagcttta tattgacctt tctttgtttt tggagttctt ctttttaatt
     5701 cttctaacac tccatcaggc agtcttttat aaatcaattc gtttgtccat ttaccaacaa
     5761 cacctggacg ccttttaatt gattgaacat cgtagctcca cccgttcaat ctaaaaattt
     5821 ccatataaaa atcatctgga aacatctttt gccattttag taattcttca ctaatatatg
     5881 ctttaaatat tttttgaagc tcaaaacgtt ctctctcata ttgataacca gtagcttcat
     5941 ctactaaagc agtaacacca actttcgcaa gtgagcgaac taacatttcc gctttttgtg
     6001 cagttctcat ctgagcttct gatttgatag ctccaatttc tctagctttt aaatataaat
     6061 ctgatactag cggtaaaatg ttagcatcaa aacctaattg aatcctacca tttaaatccc
     6121 tatattcaat tttgttgatc acacccctta aatcctttga tatcaatggt tgaaggtttt
     6181 ttgcatccat aaaagtaggt attccgatca cacgggtatt gcctctacta ggtctgtcca
     6241 atgctttaaa aacaccagct tgggtgatga ttcgctgacc gttatttaaa actgctacat
     6301 caatagttgt attatctaag actaatttac cctcgtgagt tgcatgtaaa acttcatttt
     6361 ccatattttc tcctaaactc attgacaacc atagcttcgt tcgctattgt ttttaatttg
     6421 taaactttca taaatctgta aacgttaaag tctttaacgt cgtctaacgt ggttcagata
     6481 ttacacatag ttattaattt tcctatttac gtttcgactg gggtaaaatg gcacgttata
     6541 caaaatgacg tttaaataat ttatggcttt caatgttctg atcaacatct ttctcatccc
     6601 aaatttgcaa ctcccaagga tagtagtgat tgctcttatt cttgaaataa acgtgtattc
     6661 ctgtgtaatt atctttatct cgtaaatacc agttttttaa gtcaaactta tctttccatt
     6721 catcaagttg ctccatcact tgtgaaatat cctcagaaga taaaatgata cgagcgccaa
     6781 aaatatcatt aagaatagaa ttcacaggat aaccatcttg tcttttggaa aaacgctcaa
     6841 ttttgtctaa gatgctttct gatgttttga cacgatagac gtaagggata tctttgacat
     6901 cggctttcat caaataatca ttgatggatt cgtgtagatt taaacgatag tctaggatag
     6961 ccttgacagg tacttttgaa aaggtatgtt ttagattaat cttttcaact ttcccagttt
     7021 caaaataatc ttgcgaataa acaaggtgta ttctattaat ttccgagatt aagcgttcaa
     7081 ctttttccag catattaact ctccctgtaa ctgccgacaa cttttccgat aatacggaaa
     7141 tcactatctc tatctatgag tatatcgtta tacttgtcgt tcagactatg cagaaatgcc
     7201 ccctctgcgt gtataagtag ctgtttgata taagcgtcac cgtaatactc aaacacgcct
     7261 atatcgccat ctacgagctc tacggatagc tttacgaaga catagtcacc agaatgataa
     7321 tcgggttcca tagaatcacc atagactggg ataacaaaat cagcgtcata atcgactggt
     7381 aattcaattg tttctacttg cacgtcattt agatactgac ctgtaccagc tgaagcagcg
     7441 tggtcgtagt aattgtaaga gaataattct actactgtat tcttactatc ttctactgtg
     7501 ttttgttcag ataaaagatt ttcaccgtaa cttatccaat cactatgacg aggttctttg
     7561 agccctttgt ctaattcgat gacaatattc gaaagagctg aaatattgtc atgagaatat
     7621 acttctacta ggtctgactt ttcgacatta aaatagttag ccatcagttc gattttgtcg
     7681 atccttggat aagttttagc attcacccaa tctaatactg tgctatattt aaagttcatt
     7741 actttcgaaa actctttagg gttcatattc tttttttcta aatgccttct tatattagaa
     7801 gccattatct ttttatttcc gagcgaccca ctcataataa cacctcattt ctagttgtat
     7861 tataacggat aaaccgtgct tttgtcaaaa aataatttaa aaaataaaaa aatagcaatt
     7921 ttgtgttgac aacacggttt aaccgtgtta tactataatc aagcttaagg aattaagcaa
     7981 aacgaaagga ggtacagcta atgaaatcta ggctaaacaa aaagcctaaa cacaaagaag
     8041 tcgagttgga aattcacatt ctttggttta agcttaggat tcactactca atagagtggt
     8101 gacaatacca agagggctaa gaagccctct cccctaacgg ggtaagttta gtttagcaca
     8161 ttggctgtat ctccgcaaga atgaaaggag agtagatgtg gaagaatcta tattgaactc
     8221 taagcgctac acaaaacttg tcgtagagac agaagatggc aaaaaaatag cagaagttac
     8281 tttgacggaa gcaacccctg ctgatggata cgtgattagg ttaaccccta attatgacta
     8341 acctttaggt gggaatggat cgttaccata gctgttacgt tcgcgaattc gtccattttt
     8401 gccatggata ataacttcac tgctattgtt tttagcgata ccagtagcga tttcgacagc
     8461 ttcggcttgg gttgttgtgt gtttggtcgc acgactgttg cctgcacctt ttatatccca
     8521 accgccatta gggttaggta cgacatgttg atttttagcc attagcttat cctcctttcg
     8581 ttaaggataa gttgattata acatttttag gaggtacaaa atgaattgga aaaaactaat
     8641 gtttggcgat ctagaacaca cgtttactag tcgtaatggc aaagaaaaaa caagtattga
     8701 atttgaaggt ggcgtattgc cagcactgtt agtgctaggt ggtatcactt ggctgatcgc
     8761 ttggtttatt acaaaataaa aactcccaag tgggagtgga aaggagaggg gtcagaaaaa
     8821 tgattaatct tgaagaaaaa gttcaaaaaa tagaacagaa cgaagaagaa aatacaacct
     8881 caatctcatt cttaagaggt tatatcaaag gccttgagga gcgaattagt cgtttagagc
     8941 gttcgaaggg ctgttaaagg cattagggta ttttgtttgt aactcaaaaa tcttttctaa
     9001 cttttctggc tcttgaaaag ccatcataat taagtcttcc tgagtcattg gtcttttttg
     9061 agtagaatta ttttctattt tatcagataa attttcaatt gcatcataga tgttgttaat
     9121 tgaagttttt aattggataa tatcttgcac tgaagcactt tggtcgtttg gttcatggat
     9181 tatagaaaaa tcaatgtttt cttctgtttt ttgaattcgt ctaattgttt cttggacaga
     9241 atcaagcaca accttacttt cagagttttt gagatcgtaa tgaatggtat ttatagttct
     9301 aatatcaaaa gggatatctt catcagtctt ttgtctaagg aaaatagtag gtaagttcaa
     9361 cgcttttcta aacccaagtt ctaaaaagac attcgggtta tttcctgtta tatcaacaat
     9421 taccaagtct gctgtggata aagcatcaaa tattttatcg tcaatcctat ctgaatgata
     9481 cagttcgtca gctctggtca cttgatactt ttcgatcaat gcaggtttaa ttatataatt
     9541 taaaacactg tctgagtggt tccgaatttc gctactttcc tcaccgatag cagatactac
     9601 gaaacaagat tttttactca taataatacc tcacaatttt tatttaaatt ataccacaga
     9661 aagaaggtgg aatagatgcg ccccaaaaaa tatccgtata gccaaaaaaa ctatccagcg
     9721 ccgaagcttc ataacatcat tgaaaccgat aatcatttct tgatagatga taaaaggatt
     9781 cattatgtta ttgaagattc tgtaaagaca aaaacccttg gcgatggcta tgttgaagta
     9841 acactttcca taattgccaa gagttttaca aaatcacaag gttagtttgg cgatagtcct
     9901 tgtgatagaa gttggtgtaa gagggatacc ttcttcttta aggatattct ttaaacggct
     9961 cgaaaaatta ttatctttaa gactttgaag atataagtaa ccagttgttg ttacgccatc
    10021 gaaaaaatag aaagtaatat cttttgttgg gcgacgtttt ccttttatca agcaatcatc
    10081 aagtaaatta tcaattactt ctaaagtgcc tgatattaca atgtttttat ctaagtcttt
    10141 agagaatttt acaaattcac tgttaagact tagattatca aataagctat aagcgtcgtc
    10201 tggtttggtt tccacaaagg tttccaagac aattgtataa agtttgaaat agtcaaacat
    10261 cacattcacc tcctttctgc tcacattata gcagattaga ggtactaaaa acagatagaa
    10321 agggggtggg ggaatgacga aaatgacgtt gaaaatgctt agggtttcga agaattggaa
    10381 ccaagaaaca gcagctaaaa aactaaaaat ttcagtttcg aaattaagca attgggaaaa
    10441 tgcaaaaacg tttccagatg ctattgaaat aaataaaata gagaaactat acgatgtcaa
    10501 ctactctgat attatttttt accaacaaaa cacggtttaa ccgtgtaaat caatgggcaa
    10561 cacaacacta gaaaggacaa tatgaatgaa gtaacattat caaataactt gcaacagatt
    10621 gaactagatt taagacaaga aaacgaacag attggaaagt ctatttggaa aataggttgc
    10681 atgctaaaac atgttaaaga aaatgatttg acacatggtc aatttatgga ttggtacaaa
    10741 aatcttgggt acaacaagaa tttcgttagc aaggcaataa cgatagctga caaactttca
    10801 aatttcccga cgttgggaaa tatcggaaca gaagctctct acctcatagc caccctacca
    10861 gacgagcaaa agcaggaaca gattgaatgg attgaaagtg gcgataaccc aactgtcaga
    10921 gagttgcaag aaatcaagcg agaaaacaac cgcttaaaat cagaaaatgc ccgtttagag
    10981 caacaaaaag agaatttagc agagcaagcc ttgagtgcta aaatcgttga aaaagaagtt
    11041 atcaaagaag ttattccaga tgattatgaa agcactaagc aacttaatca gactttgctt
    11101 ggcaagaata aagaactaag taagatggtt gatgatgcct tgcagcacga ggagtattta
    11161 aaaagtcaac ttaaagaatt ctatgctaag cgtgatgagg tcaatcataa atcagctaag
    11221 tatgatgagc taacagaagc gattaaacag tcagaaggta aactcaatag ctatcaaaag
    11281 aagattgcat catacaagaa tatcactgaa ctactcaaaa aaggcgattt gctattactt
    11341 gaaatgagcg gacttatcta tgctgatgaa acgcactata tccagcgtga cgggcttatt
    11401 aagcaagagt tcgatagctt ggtcgataga ggtctaaagc tctttaatga ccttgatatg
    11461 aagcgtaaga acactgaaat tttagaagga gaagtcttat gaatgaacta acaacaactc
    11521 aacaactgat tgaaatgtca aaaatgcaga cagtgacttt agaaaaggtt gataacttag
    11581 aaaaaggatt attgcaagcg cagaatgata ttcaagaaat catggataca tcttatttac
    11641 atcctggaat tattaatatg attactaaaa aacgtcgtaa acgtgtcatt gattgtatgg
    11701 gcggaaaatc tgcaaaagct tataaaacct ttaaagtaga cgaggaaggc agaaagcatc
    11761 gtttttcaag tgaagtattc cgagaaatgg aacttgattt caaagcagaa tttgatctca
    11821 atagctacgc tgaattgtct aaatctaaga aagaagaggc gcttgaatat atcgctatgt
    11881 gggaaccgtg cacgaatact aaacgaaaaa ttaatctgtt gaacaaacag actgaacttg
    11941 aactgattgg gtaagaaagg aacaaaacta acgaagggag aaaagaatgg aagaagacat
    12001 caagcagttg atcactgaaa atatcttaga atttttaggt tctgactatg gaaaaggttt
    12061 cttaaccggc ataaaaattg ccgttgagat tattgccaaa caaataccga caccttcaga
    12121 agaggatatc ggtcatgata gttaattttt gcgctgctgt tcgaaagctt gttttaaatg
    12181 agttgccatc atttgctgaa cagcaagaac gaaatccata ctatggagcg ttcatacaaa
    12241 atcaaagtgt atatcactaa ggagaaagaa catgattact attttaaaag aaataaacca
    12301 aacactaaaa gaaatcctag cagaattaaa agaacctact gtggtaacag tagattctga
    12361 aaagttaagt aatacgctaa ctactgaaca gaagatacgc cgattatctg gtcaataaac
    12421 agaacaacaa atggaaggtt caaagtagat tcttttgatg ttgacaaagt agcatcaaca
    12481 agcaacatat atctttcgtt gtcatcttta ttttctttac gatattccaa gaattcaagg
    12541 acatgagcat acttggcttt atcaggattt tcaggtaata aagttccagt gaatattcca
    12601 ctcgctgtaa tcacattaag cttacaattg cttttttccg aaaaaatagc gacatctgca
    12661 ataagattag tttttaaaac gtcgttcatt ataatcacct cctttcgaga tgattatacc
    12721 actaaaaaag tccgacggga atcggactca aaacaaactt aatttactta attatatcac
    12781 agaaaggaaa caatatgcta gcaaaactta aaagcggtat cgaagtacct tacgaagagc
    12841 tttggcttaa tgataacgac ttatccgaat ttattggaaa gtcatttgac caaacgcagc
    12901 gattactaag aaagatgtac aaagacagaa attatcgcaa atacattgac aaggttggcg
    12961 gtcgttcaac aaaagttaaa aaatttgaag aatggagaaa attacaaaat gaaaaaatta
    13021 tttaacttta tttttacaaa accaaaaaaa gaagaaccaa aaccaaaaag gacaattgaa
    13081 acacacggct gggaagttgg tgcgaaagct tatgatgatt ttcataatta tatgaaaggt
    13141 ttgaaacatg gatgaatgga atccaatttt aattaggaac tagatggttt gcctggtaag
    13201 caggtttggt aggaaataca tagtcctttg acaactgaat atgggtgcgt tggagtaaaa
    13261 tatattttac aaatttccgt tttaatacgg ttaaagtgta ttttttcttg atatttcgtt
    13321 tttttcgtag tatactttag gggaagtgaa gaatatttag cacatttagt gccaaaaaga
    13381 aatcccctag taccgcaaat actaggggat ttttctagtt tcctagaaga cactaatcat
    13441 cgttgtctag ccacttttgg actagcaaca agacgatgcc gaccaataat ggtgcgatga
    13501 ttgttgtgaa gaatatttcg cacatggttc tcacctccaa tctaaggcgg tgtagtagtg
    13561 ccgagtagta ttatagcatt taaagcttct aaaagctacg agatttcaat cctgatagct
    13621 tctgatgaca agaacggtaa aatgtatgtc tagataacag actgtctcag aagtgatttt
    13681 agggctgttt tgagagaaag aaaaatattt aaaaatgttt aaacaaaacc attgacattt
    13741 gtttaaacat aagttataat taaatcatgg ttaaggaatt aactaaatcc aacggaaagg
    13801 aaacggagct atgcgaagtc gaaaaactaa gcaaaaagaa aagctctcgc tgactgataa
    13861 atttgtgatt atcggcataa tcatagaaat aatcagattc atacgagaac ttctctaaaa
    13921 gcaacgaaac cagagggcga aagccctcgg tggtttcgtg tacttcgatt ataacatagc
    13981 tcctacaggc tagcaaatga agcacgaaaa aagaactgaa attttgttag ggattttaat
    14041 tgtcttgcaa ttgttaaatc ttgccttgag ttttcgatag gtgacagatt tggcaaaaat
    14101 ggggcgacct actagcaatc caagggataa gtatatcggt gtcagagctt caaaaaatga
    14161 ggtagaaatg cttgattttt gtaccgaaaa gactggaaaa agcaaaaccg atatcctcat
    14221 ggaagggctc gaaaaagtct ataatgagct aaaagggtaa acaaaaaaac accacgtaac
    14281 tactcaaacg ccaatccgat tagttacgca gtgccaaacg cacccatatt caagaagaat
    14341 acaggatacg cttatattat aacaacgtac ctgtatttgt gcaactcaaa tttataggta
    14401 cgctttttgt gtgcctcaaa ttcgcaggag gactgtatga aaacttacac attaactgaa
    14461 gaagaattga atgaattagt agccgagcgc atgaaacaag cgaaagaaaa acgcacacca
    14521 caggggctat ttaaagatgt cggctttgat gatgagttaa ttccgatcaa caataaatac
    14581 ccaaaagtac tcaagaaatt aaatcgtgaa cgtgcttata aaccagaaaa acacgtcttc
    14641 aatcagacac caaaagtttt tggtgtggac aacgagatta gttatagcaa aattacaaca
    14701 catgacgtgc acaaccatat tcgtttgctt gtcttaaatg tctttggtaa aagtcaaaat
    14761 aaggaagtat tgcctgagga atacgaccaa gcaatagaac tttacaatca attaaaagag
    14821 tggtttgtgt ctagctatga taagcgatta gagggattgg tactagaaga tgattaaaaa
    14881 aatatgcgtt aactacttac taaaacagat tgacaaaagc aaactagaaa caagagataa
    14941 agcgaagttg aactacttta tcacactagt ggactgcaag ttaggaggat aaattgggta
    15001 aagaaaaaac taaaatctac ttttggctaa agttcgacaa gaagtttttt gaaaatattt
    15061 ttatcaaacg tttaaaaaag atggctggcg gagacactat gacagtcttg tatattcgtt
    15121 tgatgctaga aagtttagaa acagactgca ttttatatta cgaaggatac tttgataatt
    15181 tggtagaaga gcttgccatc aagttagagg tgtctgaaga cgatatcaat atgacattag
    15241 cttacttcac aaaatgcggt ctaatccaga tagacggtag cggtaacgca caaatgcccc
    15301 aagctaaagc tatgctggaa agcgagacga actgggcaaa atataaacga gaaactagaa
    15361 aaattggaca aattccaacc gatgtccaac caatgtccaa ccaatgtcca acagagatag
    15421 agatagatat agagaaaaag aaagataaag agagagagtt agataaagag aaagaatata
    15481 ttgtcgagca gagcccgact gaatatctct ttccagactg gttagaagag aaatatgtcg
    15541 aacaagtcaa aaaaggtaat cccaaaaatt ttgattatcg tatcccaata gcttatctca
    15601 accaaaaaat gaactctaac tataagtttg taaaaacaaa cacagattta gtcaaagcga
    15661 gactaaaaga tagttatact ttagaagatt tcaaagctgt catagataaa aaatgcagcg
    15721 agtgggtaaa ttctgacatg gaaaaatatc tcaggccatc aaccttgttt aatgctagca
    15781 agtttgagag ctatctcaat cagccagaag ttgctaaaag tgattattac cagaagcaac
    15841 aaggccaacg attttcgcaa gctgagttag atgagcttaa gaaaccagat ccgaaatatg
    15901 gattttagga ggtatctatg gcttttgggt taatgacaag agagagcatg ctcgagaatg
    15961 gcattattag agatactggg aaaacatgcg aaaagcacga gatgccaatt tatgctagga
    16021 aaatgccaaa tcatggcaat agagaaacag aattttgttg gcaatgtaca acagagtata
    16081 tccaaacgaa aagtaatgcg gttgacattg cgtacaacaa ccagtcgttg ctagctaagg
    16141 gttataaagt gttttataaa gagagcgttt tatcaaagga aattgctagt gctacgttga
    16201 aaaactacaa ggaacatagt gctgtagata caaaagcgct aaactatgcc aaacgaatca
    16261 ccagagatta tgttaaagga atggaaggta actccctctt acaaggacct ccaggggttg
    16321 gcaagagcca cttgtctatg agtattgcta aaaatattaa cgagatgttt aaatcttaca
    16381 atcaatcaaa gagtgtgata tttgtttcgg tacctttgtt gtccggacta gtcaaagata
    16441 cattcgatta tgacgataaa aaaaatagca aatattcgca agaaagaatg tcaaagcttc
    16501 tcatcaattg tgattatctg atacttgatg acttaggcaa ggagtcaacc acaggtaaca
    16561 ccattaaatc tgctagcggt tggacatata cgtttttatt taatattttg gataatcgga
    16621 caaatactat cattaataca aattttagta gagctgagct tatgaaaatc tacgatgctg
    16681 cttttgtcga tcgcataatc aaaggtgcaa aaaacaatat ttttaaatat ccagataatg
    16741 cagaaagtaa gaggttctga tggaactaac attaacaaca tttttcagct tgtcagaaga
    16801 acatgcagca aaaatcatgg ctctagacga acctagtcga aataaaaaaa ttgaagaata
    16861 taggcagtta agactgcgca gagggaggat tgactttgga aaatagacca gatttaaaat
    16921 tagtagctga attagaaaat aggattaaag acttaaaaat tgaaaatgaa atcttaaagt
    16981 ctaaaaacat tgatttgtct gaagatgtta aacatttaaa atataaatgc ttcgaaaaag
    17041 atagttctat agtggatatt atggttaata ataacgacct aagaaaagaa aacaatgagt
    17101 tgcgagaaat gtttgacttt ttcaaagata gactagagaa atttgtaggt agttcgcatg
    17161 gtagaaatta ggattaatgg cgaacttgta acgtttgacg gtaattttag ggatgctttt
    17221 atatttacaa ttgactattt acgagacagc gaagagccta cgctaaggca gacttacaac
    17281 gaatttaaag actatacaga cgaagacttg atggaataca ttgaaacgga atttgatgta
    17341 aaacctgaat taattgtcaa tcggagactt gatagtaaat gggcttttaa atctcacatt
    17401 ttggaagact aaatatgagc gaagagttat acgagtctac tcgttattgg caaagtagat
    17461 acagcgactt gatgtctgat tatcttaaag aagctgaaga aaatatagag ttaaagaaac
    17521 agttgaagag attaaaagcc gaaaattggc aattgaaaca tagaaagagg aaataaatgg
    17581 cttatttata cgagttacag ggtatttatg cgcaattatc agctatggat cttgatgatg
    17641 aaacatttca agacactttg gatagcatcg attttcaatc agatttagag aataatattg
    17701 aatattttgt aaagatgtta aaaaatacac aagctgatat tgaaatgtac aaaaacgaaa
    17761 aagaaacttt ttacaaaaag caaaagcaag cagaagccaa agcggaaaaa tacaaagata
    17821 caattaggct agcaatggac ttaagtcaaa agaaaaaagt tgatgctgga atgtttaaag
    17881 tatctttgcg aaaaagtaag aaggttgagg ttttggacga aacaaaaata ccttttgaat
    17941 acatgcaaga aaaagttgaa tacaaaccaa aaaaagatga aatctcaaaa gttttaaaat
    18001 ctggaattga tatatctgga gttcaactaa tcgaaacaga aagtttacag gtgaagtaga
    18061 tgagcatgac ttttgcagaa ttgcagacaa aaatgcaaat aacaaaaaca acaaaacaag
    18121 gcgttaaata tacatttcgc aatgcagaag atatttttac acactttaaa acactaaata
    18181 gcgggtggga gttaacggta tctgacgaat tggtggaatt gatcggcaga atttttatca
    18241 aagcaacagc aacagctaga cttggtgatg aacaacacca agcgacaaga tgtgctgagt
    18301 tggacagtgt gcctgtttta aatactaaag actataaaac aggagaacct aaacaaatac
    18361 aacaaatgca agttccgcaa tggactggtg cagtgagttc gtacgcaggt aagtatgcct
    18421 tgcaagggct gtttggaatt ggtgaggaag atgtagatgc gattgttaca gaagatacgc
    18481 aacgcaaaga acaaaaaacc tcccgaccga caacctctaa aactcctaaa ataagcaata
    18541 tccaagtcga gacttacaag tctgatttaa atgatattgc gaaagccaca aaccaaaacg
    18601 ttgaagagtt aacaaaatgg ctaaccgata ctttaaaagt ggggacactg gaaaatttgc
    18661 atacggaaca cattgtttcg gcagacgaat taatcaataa actcaaaaag aaagcaggac
    18721 taaaaaatga ttaataatat tgtacttgta ggtcgcatga ccaaggatgc cgaacttcgt
    18781 cacacgccaa gtcaagtagc tgtagctacg tttacacttg cagttaaccg cagattcaaa
    18841 gagcaaaacg gagagcgaga gacggacttc attaattgtg ttatctggcg acaatctgct
    18901 gaaaatctag caaactgggc taaaaaaggg actttaatcg gtatcacagg gcacattcag
    18961 acacgcaatt acgaaaatca gcaagggcaa cgtatctatg taacagaagt tgttgcggaa
    19021 aatttccaat tattagaaag tcgcaatagc caacaacaga ctaatcaaag cggcaatagt
    19081 tctaattctc attttggcaa tgccaacaaa atggatattt cagatgatga cttaccattc
    19141 taaatatgac gaagccgcgg aagcaaagga tatatgcaat atatgacgac gacaagtttg
    19201 tcgacgttgg cacaaaagaa gagttatcag cacggcttgg tatcaaaaaa gcaaccatag
    19261 aacagtacat gactaaatct tatcaagcta gacctagctc aaaaagaatc gctatttttg
    19321 taggagttga agaaattgaa ttttaaaaca gagtttgaga taccagtaga gccaaaacca
    19381 caaacaagac caaaatttag caagtgggga acgtatgaag accctaaaat gaaaaagtgg
    19441 agaaaacaag tcactggttg gattgaaaaa aactataatg ggccattttt tgataactgt
    19501 gtaaaagtag atgtcacgtt ttacatgaga gcgccacaaa cgctaataaa agagcctacg
    19561 gcacgctcaa aaagtaaaac cgtacaaata tatcaaaaat ttataaacga gcttatatgg
    19621 cacgtaaaga agcctgatat tgataaccta gttaaagctg tttttgatag tatttcagac
    19681 gcgggttatg acaaaataca aaaatcgggg attgtctggt cagacgataa tattgtatgt
    19741 gacttaagag caacaaaaaa gtatagtcca aaccctagaa taaaagtaaa aattgaggaa
    19801 atagatgaac gaactaacgg ataaatttta tagtatcttt gatagcagta ttttgagacg
    19861 tgtcaaagag ttaaatctag atgataaaac atcagaacgc ttaagactaa atatctcaaa
    19921 taacaagcgt agaaatatac tgccaaggcc ttacgtaatc gaagcattta aagattattt
    19981 tgataaagac acttatgtgc agctgtatct taaatcatac agagagtatc acaacccaaa
    20041 cagccatgaa actgatattt ttataaagtt aaacaaaaag cacagagata caaagttaga
    20101 ccattacaag aaagttaagc gattgatgta cgcagcaatg actttctgag gaggtatagt
    20161 atggcagata aaataaacgc agagagtatg caagctgcat acaacgaaaa ttatcaaatg
    20221 tttttggcta aaaatgcaga ttatgggaac tcgttcgaaa agtctttgaa tgacttcgga
    20281 tttatcgctg gtgtcgtccg tataggcgat aaatacaaca gactatataa tcttataagc
    20341 agcgacaaaa acgtctcaga aagcctgtca gacacgttaa acgacatggc taattattgc
    20401 acaatgttgt caatctggct agagaaaacg gagaatgcaa atgacacacg tagttagggt
    20461 ttacgatcac attggtggac gagtgttgcc tattactaga agggttagaa ggatgacaaa
    20521 atatattgaa attaaagatg attggaaaaa agcagtagac catttaaatg attttatcga
    20581 aaagtatagc tataagaaga ttgttgtcgt tgggtatcag gttgttagaa accccgagac
    20641 aaacggaaca tgtactcatg tgttagttaa agtggattga tgaaaaaaat atcaaattcg
    20701 aggtaacaga atgacaaaag aagaagtaat tgcatttctg acagaacagc gtgatttgcg
    20761 gcttgttgca tatgagtggg gaaaagacaa tctgtccgtt tttgcgagat ggcaattaga
    20821 acaagcaaat atgtatttag atatcattga gtggatagag gaggtgacgg aatgactgaa
    20881 caacagatga ttgattgctt gctttatgag ttagcaaaaa aagacaaatt gaacattaga
    20941 cgaaacaata tcataacgtt tttatcgatt gtgctgatgg ctatatctat tttaaacgtc
    21001 gcactgcaag accactacaa gctacaaatt acaggattac ggacacagct aagcagaaca
    21061 caaaagcaac ttaaacgtgc tagcgagcaa aatcagagac agactaagcg gattgcggaa
    21121 cttacaggaa atgggggata gggtatgatt gacgaaattt taaaaagact taataaagaa
    21181 tttgacaatg atctggataa ttacgaacaa gaaagatacg ctggttacat ggacgcaata
    21241 ggtgtagcaa ttgaaattgt tgaagaagtt aagcgaggta gaaaatgaat attgaagaag
    21301 cgaagaaatt gatagacaaa cagtctattg gtaaaggtgg tgtcggcgac attccagtag
    21361 tgaaaacaca tattgtaaaa gtattactcg accaactcga caaaccaaaa ctagaagtac
    21421 cacaaatgat agctgatgtt attgaaagct ttgacgaaga tgtgaattat ttgcacgaac
    21481 atatgagtta tcagtctgat gaagttagag agtggctaac tcacaatgaa cgtgagtttt
    21541 atgaagcttg gctagcttat ccaaatatca cagtcgaaaa agaaaatctg tatactgttg
    21601 agatacctaa tccgaatgaa agacagttaa gttttgtgct gatgagacag cttagcggaa
    21661 atgtaagtat caaagttatg catagagata acttagactt actaaagata gataacaatt
    21721 tacaactcac agaatccgaa atccgcaaag attttgactg ggcttggcag tttagaaaag
    21781 atgtagaaaa tgaatgaaag agagttaaat aaaaattatg aattatgata aaaataaaaa
    21841 tgatgctaaa aagaacttta ttatagcttt agttctattg ccgttcggct tagtattatc
    21901 tggatttgtt attaaatacg gttggaataa cattttatca acaattgatg gcgtaccatc
    21961 tatcaattta ccgcaagctg taggaatcaa tgtgttaatt agtccttttg cttctaaaaa
    22021 aaatacagat gaagattttg ctacagtgat tgcaagagcg tttatttctc cgctagtcgt
    22081 attgttattg ctttggatag tgactttgtt tatgtaatat cttgaggtaa cagaatgaat
    22141 gaagaattag gagtgttggt aaatcccaaa cgtgcgtatt atgtaggaaa taatcgggac
    22201 ggattaccag tatacacagt caacaccgaa tatgcacaca aatgcacacg caaagaagca
    22261 gaacagtttc cgcagtttag atgggtgagt ttggaggagt tgagatgaca tatttaaaaa
    22321 aattatttga agccgttaaa tttctcaact ctatggaaaa acagaacaaa aaaataacga
    22381 ttactttcca taataaaaaa gttcattttt acgagttaga agaaattaca gagtcttcga
    22441 g
//
LOCUS       DBJYSH010000017        22428 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.017, whole genome shotgun sequence.
ACCESSION   DBJYSH010000017 DBJYSH010000000
VERSION     DBJYSH010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 22428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22428
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.017"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            complement(47..292)
                     /locus_tag="ACUBV3_000556"
     CDS             complement(47..292)
                     /locus_tag="ACUBV3_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2129 domain-containing protein"
                     /protein_id="HHU3847086.1"
                     /translation="MNKQERLGMSVYLYYNRDARKLYKYGDVHYHSRRLRYLVIYVNK
                     EDIVSVSKEIKHLKFVKDVRLSAIDDIDQDFVGNLYR"
     gene            complement(392..982)
                     /locus_tag="ACUBV3_000557"
     CDS             complement(392..982)
                     /locus_tag="ACUBV3_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HHU3847087.1"
                     /translation="MIPVVIEQTSRGERSYDIYSRLLKDRIIMLTGQVEDNMANSIIA
                     QLLFLDAQDNTKDIYLYVNTPGGSVSAGLAIVDTMNFIKSDVQTIVMGMAASMGTIIA
                     SSGAKGKRFMLPNAEYMIHQPMGGTGGGTQQSDMAIAAEHLLKTRHTLEKILADNSGQ
                     SIEKVHDDAERDRWMSAQETLDYGFIDAIMENNNLQ"
     gene            complement(1107..1736)
                     /gene="upp"
                     /locus_tag="ACUBV3_000558"
     CDS             complement(1107..1736)
                     /gene="upp"
                     /locus_tag="ACUBV3_000558"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002883819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HHU3847088.1"
                     /translation="MGKFQVISHPLIQHKLSILRRTTTSTKDFRELVDEIAMLMGYEV
                     SRDLPLEDVEIQTPVATTVQKQLAGKKLAIVPILRAGIGMVDGFLSLVPAAKVGHIGM
                     YRDEETFQPVEYLVKLPEDIDQRQIFVVDPMLATGGSAILAVDSLKKRGAASIKFVCL
                     VAAPEGVAALQEAHPDVDIYTAALDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(1846..3015)
                     /locus_tag="ACUBV3_000559"
     CDS             complement(1846..3015)
                     /locus_tag="ACUBV3_000559"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HHU3847089.1"
                     /translation="MITYDFTSLPERFSSNAIKWKAVQKDQEILPLWIADMDFPIFPE
                     MSEAIEDFSHQMVFGYDSPKDSLYQAISNWEVQEHGYQFDKKSLLLIDGVIPAISVAI
                     QAFTKEGDAVLINTPVYPPFARTIKYNNRHLVSNSLLNNNQYFEIDFKQLEKDIIENN
                     VKLYIFCSPHNPGGRVWTKGEIQKIGDICKRYNVILVSDEIHQDLVLFDNVHHSFNTV
                     DSSFKEFSVILSSATKTFNIAGTKNSFAIIENEKLRSDFKKRQIANNQQEISSLGLLA
                     TEVAFTKGKKWLKALKMELEGSIEYLYEQLTQKTHIKVMKPEGTYLVWLDFSAYNLTH
                     LEIQEKLRYDAKLILNDGLTFGKEGKKHARINVAAPRSVIEEAVLRLVTNFRKND"
     gene            complement(3151..3699)
                     /locus_tag="ACUBV3_000560"
     CDS             complement(3151..3699)
                     /locus_tag="ACUBV3_000560"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001019793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0141098 - tRNA
                     (cytidine(34)-2'-O)-methyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HHU3847090.1"
                     /translation="MNIETLTQKNHRSDSGRNHIVLFEPQIPANTGNIARTCAATNAP
                     LHIIRPMGFPIDDKKMKRAGLDYWDKLDVSFYDSLEEFMLSCRGKVHLISKFADKVYS
                     DENYNDDQDHYFMFGREDKGLPETFMREHAEKALRIPMNDEHVRSLNVSNTVCMIVYE
                     ALRQQSFPNLELSHTYENDKLK"
     gene            complement(3872..5224)
                     /locus_tag="ACUBV3_000561"
     CDS             complement(3872..5224)
                     /locus_tag="ACUBV3_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HHU3847091.1"
                     /translation="MENHNSIKQTYGLMTTIAMIVGVVIGSGIYFKVDDILKFTGGDV
                     FLGMVILVLGSFSIVFGSLSISELAIRTSESGGIFSYYEKYVSPALAATLGLFASFLY
                     LPTLTAIVSWVAAFYTLGESSSLESQIILAAVYILALSLMNIFAKRIAGGFQSLTTFV
                     KMIPLVLIALIGAFWSDKAPQLPQHLTAIQPSNVGWSWVSGLVPLYFAYDGWTIFVSI
                     APEVKNPKKNLPLAFVIGPALILLSYLAFFYGLTQILGASFIMTTGNDAINYAANIIF
                     GPSVGRLLSFIVILSVLGVANGLLLGTMRLPQAFAERGWIKSERMANINLKYQMSLPA
                     SLTVMAVAIFWLFVHFMVTKFNLLPGSDISEIAVVFNNTSLIILYVLVLSLYLKKDIK
                     NKFTGLVSPILAILGGLILFIGSLLSNFFTVLIFQCFCLLFCLICHYIYQKNNPKTHE
                     "
     gene            5430..6779
                     /gene="trkA"
                     /locus_tag="ACUBV3_000562"
     CDS             5430..6779
                     /gene="trkA"
                     /locus_tag="ACUBV3_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HHU3847092.1"
                     /translation="MRIIVVGGGKVGTALCRSLVAEKHDVVLIEKKENVLKRVTKQHD
                     IMGIVGNGANYKILEQAEVKNCDIFIAITDRDEVNMISAVLAKKMGAKETVVRMRNPE
                     YSNPYFKDKNFLGFSSVVNPELLAAQYIANTIEFPNATSVEHFANGRVMLMEFKILEG
                     NKLCHTSMSQIRKKFGNIVICAIERDGKLIIPDGDATIQVKDKIFVTGNRIEMILFHN
                     YVKNKVVKNLMVIGAGRIAYYLLNILKNTNTHVKLVELNQEQAEYFSQEFPNVPVVHG
                     DGTAKNILLEESVTSFDAVATLTGVDEENIITSMFLESIGIPKNITKVNRTSLLEIID
                     DKQLSSIITPKRIAVDHVMHFVRGRVNAQDSNLEAMHHIANDRIETLQFEIKETSKLA
                     NRSLASLKLKQNILIAAIIRNNKTIFPTGEDVLTVGDRIVVITLLKNITRTSDMLAR"
     gene            6784..8223
                     /locus_tag="ACUBV3_000563"
     CDS             6784..8223
                     /locus_tag="ACUBV3_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HHU3847093.1"
                     /translation="MNKSMIRFLLSKLLLIEAALLAIPLTVGLIYREPQSVMMSIVIT
                     MIILIILGLLGSLFKPKNYHIYTKEGMLIVALCWILWSFFGALPFVISGQIPNIIDAF
                     FEVSSGFTTTGATILDDVSVLSPALLFWRSFTHLIGGMGVLVFALAIMENSKNSHLEV
                     MRAEVPGPVFGKVVSKLKKTAQILYLLYLLMFAVFAVILYFAGMPFFDSIIIAMGTAG
                     TGGFAVYNDSIAHYNSPLITNLVSIGMLIFGVNFNLYYLLLLRKIKAFFGDEELKNYL
                     RIVAIATFMIALNVIGMYDNFRQGLEHIFFEVSAIITTTGFGVTDITRWPLFSQVILL
                     FLMFIGGSAGSTAGGFKVMRSLSLAKIARNQVLSTLYPNRVMSLHINKSVLDKNTQHG
                     VLKYLTIYLAIFMALVLVLTLDTNDFLVVVSAAASCFNNIGPLLGSNETFSFFSPFSK
                     LLLSFAMIAGRLEIYPVLLMFIPKTWSKL"
     gene            complement(8247..8498)
                     /gene="yidD"
                     /locus_tag="ACUBV3_000564"
     CDS             complement(8247..8498)
                     /gene="yidD"
                     /locus_tag="ACUBV3_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HHU3847094.1"
                     /translation="MLKSFLIFLVRFYQKNISPAFPASCRYRPTCSTYMIEAIQKHGL
                     KGVLMGIARILRCHPLAHGGNDPVPDHFSLRRNKTDISD"
     gene            complement(8498..9220)
                     /locus_tag="ACUBV3_000565"
     CDS             complement(8498..9220)
                     /locus_tag="ACUBV3_000565"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001934129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HHU3847095.1"
                     /translation="MRINKYIAHAGIASRRKAEELIKQGMVTINGQVVNELATKVKAG
                     DLVEIEGSPIYNEEKVYYLLNKPRGVISSVSDDKGRKTVIDLLPQVKERIYPVGRLDW
                     DTTGLLILTNDGDFTDKMIHPRNEIDKVYLARVKGIATKENLRPLTRGVVIDGKKTKP
                     ARYNIIKVDHEKNRSVVELTIHEGRNHQVKKMFEQVGLLVDKLSRTQFGTLDLTGLRP
                     GEARRLNKKEISQLHNAAINKS"
     gene            complement(9210..9794)
                     /gene="scpB"
                     /locus_tag="ACUBV3_000566"
     CDS             complement(9210..9794)
                     /gene="scpB"
                     /locus_tag="ACUBV3_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HHU3847096.1"
                     /translation="MTYLGSIEALLFVAGEDGLSLRQMAELLSLTPSALIQQLEKLAK
                     RYEEDDDSSLLLLETAQTYKLVTKDSYMTLLRDYAKAPINQSLSRASLEVLSIIAYKQ
                     PITRIEIDDIRGVNSSGAITRLIAFGLIKEAGKKEVLGRPNLYETTNYFLDYMGINQL
                     DDLIDASSIELVDEEVSLFSMDSINTEDKENNEN"
     gene            complement(9791..10498)
                     /locus_tag="ACUBV3_000567"
     CDS             complement(9791..10498)
                     /locus_tag="ACUBV3_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HHU3847097.1"
                     /translation="MDIKLKDFEGPLDLLLHLVSKYEVDIYDVPIVEVIEQYLAYIAT
                     LQAMRLEVAGEYMLMASQLMLIKSRNLLPKVVESTPIEDDPEMELLSQLEEYRRFKVL
                     SEELANQHQERAKYFSKPKQEVIFEDAILLHDKSVMDLFLTFSQMMSQKQKELSNSQT
                     VIEKEDYRIEDMMIVIERHFNLKKKTTLQEVFADCQTKSEMITLFLAMLELIKLHQIT
                     VEQDSNFSQVILRKEEK"
     gene            complement(10498..11238)
                     /gene="xerD"
                     /locus_tag="ACUBV3_000568"
     CDS             complement(10498..11238)
                     /gene="xerD"
                     /locus_tag="ACUBV3_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017770859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HHU3847098.1"
                     /translation="MINDINNFIESKKLSLNSRKSYHYDLKQFYKIIGGHVNSEKLAL
                     YQQSLSEFKLTARKRKLSAVNQFLFFLYNRGTLKEFYRLQETEKITLTQTKSQIMDLS
                     NFYQDTDYPSGRLIALLILSLGLTPAEIANLKKADFDTTFNILSIEKSQMKRILKLPE
                     NLLPFLLESLEEDGDLVFEHNGKPYSRQWFFNQLTDFLNEKNEQQLTAQLLREQFILK
                     QKENGKTMTELSRLLGLKTPITLERYYR"
     gene            complement(11228..11701)
                     /gene="cbpB"
                     /locus_tag="ACUBV3_000569"
     CDS             complement(11228..11701)
                     /gene="cbpB"
                     /locus_tag="ACUBV3_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0180001 - cyclic-di-AMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP-binding protein CbpB"
                     /protein_id="HHU3847099.1"
                     /translation="MIAKEFESFLLSHLDHYLIPAEDVAIFVDTHNADHVMLLLASNG
                     FSRVPVITKEKKYVGTISISDIMAYQSKGQLTDWEMAQTDIVEMVNTKIEPINEAATL
                     TAIMHKIVDYPFLPVISDQNDFRGIITRKSILKAINSLLHDFTDEYTITPKNNDK"
     gene            complement(11698..12219)
                     /locus_tag="ACUBV3_000570"
     CDS             complement(11698..12219)
                     /locus_tag="ACUBV3_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HHU3847100.1"
                     /translation="MAIRKLVVMSDSHGDRDIVKDIKNHYLGKVDAIFHNGDSELPSS
                     DPIWEGIHVVTGNCDYDSGYPEVLVTKIDNTVIVQTHGHLHQINFTWDKLDLLAQQED
                     ADICLYGHLHRADAWKNGKTIFINPGSVLQPRGPINEKLYAVVTITDSKVLVEYYTRQ
                     HQPYPNLTKEFSR"
     gene            complement(12201..13175)
                     /locus_tag="ACUBV3_000571"
     CDS             complement(12201..13175)
                     /locus_tag="ACUBV3_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphate diphosphatase"
                     /protein_id="HHU3847101.1"
                     /translation="MTKTIFESKTEGNWFLGSFQAFNYFTCFGNDESYEAIQDVFHRL
                     LSTLKVEGLQLHVVQMTSDFQLLAFLVDMINQEYSRHIKVTQHKGAILVSEDDQLFLV
                     HLPKEGTSLEKFFDLKNDNNFGDTILIATHNEGKTKEFRELFGKLGLKVENLNDYPDL
                     PEVEETGMTFEENARLKAETISKLTGKMVISDDSGLKVDALGGLPGVWSARFSGPDAT
                     DARNNAKLLHELAMVFDKERRSAQFHTTLVVSAPNKESLVVEAEWPGYIGTEPKGENG
                     FGYDPLFIVGEGSRTAAELSAQEKNNLSHRGQAVRKLMEVFPKWQLEN"
     gene            complement(13172..13966)
                     /gene="racE"
                     /locus_tag="ACUBV3_000572"
     CDS             complement(13172..13966)
                     /gene="racE"
                     /locus_tag="ACUBV3_000572"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000378350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HHU3847102.1"
                     /translation="MDSRPIGFLDSGVGGLTVVKEMFRQLPEEEVIFIGDQARAPYGP
                     RPAQQIREFTWQMVNFLLTKNVKMIVIACNTATAVAWQEIKEKLDIPVLGVILPGASA
                     AIKSTNSGKVGIIGTPMTVKSDAYRQKIQALSPNTAVVSLACPKFVPIVESNQMSSSL
                     AKKVVYETLSPLVGKLDTLILGCTHYPLLRPIIQNVMGAEVKLIDSGAETVRDISVLL
                     NYFEINHNWQNKHGGHHFYTTASPKGFKEIAEQWLSQEINVERIVL"
     gene            complement(14141..14380)
                     /locus_tag="ACUBV3_000573"
     CDS             complement(14141..14380)
                     /locus_tag="ACUBV3_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HHU3847103.1"
                     /translation="MSTTIWILLIIVALFGGLVGGIFIARKQIEKEIGEHPRLTPDAI
                     REMMSQMGQKPSEAKVQQTYRNIVKHAKTAIKTKK"
     gene            complement(14538..15080)
                     /locus_tag="ACUBV3_000574"
     CDS             complement(14538..15080)
                     /locus_tag="ACUBV3_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3847104.1"
                     /translation="MSKKTTQMVSYTSILVAFAIMIPIIMPAKIIIGPASFTLASHVP
                     LFLSIFISVPVAILVALGTGLGFLLAGFPIVIVLRALSHIGFALIAAFLIKSKPSLLM
                     SKWQTLLFAVAINIIHGLLEFITVYIITMTSNSSSTYLWSLFSLIGLGSLLHGLVDFY
                     IALFIWKWMTQKLGKTLRIR"
     gene            complement(15142..15663)
                     /locus_tag="ACUBV3_000575"
     CDS             complement(15142..15663)
                     /locus_tag="ACUBV3_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HHU3847105.1"
                     /translation="MKAQERRENILTTLKGTKEAISASTLAKIFSVSRQVIVGDIALL
                     RAQQCDIISTPKGYLMSSALSTHQFTARLVCQHGIEQTEEELKIILRYQGIIMNVEVE
                     HPIYGMLTAPLNIQSQKDIDNFTAKLKVSNAELLSSLTDGLHTHMISCQDQSVFDQIC
                     EALKKAGILYIDK"
     gene            complement(15731..16420)
                     /locus_tag="ACUBV3_000576"
     CDS             complement(15731..16420)
                     /locus_tag="ACUBV3_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HHU3847106.1"
                     /translation="MNDNVIYTQSDSGLNQFFAKIYGLVGIGVGLSAAVSAIMLYMFP
                     QNMIAIMQKMPGLYFGAIILELVLVFVASGAARRNTPAALPLFLIYSALNGFTLSFII
                     ARYTQTTVLQAFITSAAVFFAMALIGAKTKKDLSGMRKALMAALIGILIASLVNLFIG
                     SGGMSYIISIVCVIIFSGLIAYDNQMIKYVYNSQGGQVADGWAVSMALSLYLDFINLF
                     LNILRLFARND"
     gene            complement(16443..16946)
                     /locus_tag="ACUBV3_000577"
     CDS             complement(16443..16946)
                     /locus_tag="ACUBV3_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003087049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HHU3847107.1"
                     /translation="MKKYQDDKDFMDLVGHLIDHPRFQKLEAIVQHHHSTRLEHSINV
                     SYTSYKIAKKFGWDASSTARGGLLHDFFYYDWRVTKFNKSHAWVHPRIAVRNARKLTD
                     LNAREEDIILKHMWGATIAPPRYKESYIVTMVDKYWAVREASRPLKRIFKKPIRFSRK
                     FLGSHNH"
     gene            complement(16984..17727)
                     /locus_tag="ACUBV3_000578"
     CDS             complement(16984..17727)
                     /locus_tag="ACUBV3_000578"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000207503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HHU3847108.1"
                     /translation="MTTIITSKSNNLIKKTKKLLQKKYRKSSYLIEGWHLFEEAEKYG
                     AQFLNIFVTETAIDRLRKPEEAIVVTDDVLKELTDSQTPQGIVAEIAFQETRWTDIKK
                     GRFLVLEDVQDPGNLGTMVRTADAANFDAVFLSQKSADLYNQKTLRSMQGSHFHLPVF
                     RVEIEQFVNFCKAEGITMIATTLSEQSVNYKNLPKYDHFALIMGNEGQGISKTMTEEA
                     DVLAHIEMPGQAESLNVAVAAGVVIFSLI"
     gene            17759..18037
                     /locus_tag="ACUBV3_000579"
     CDS             17759..18037
                     /locus_tag="ACUBV3_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HHU3847109.1"
                     /translation="MKKVHLIVSGRVQGVGFRYATYSLALEIGDIYGRVWNNDDGTVE
                     ILAQSTDSNKMTQFIQKIRKGPSKWSKVTYVDIKLDNFDDFNDFKMRN"
     gene            18122..19054
                     /gene="yidC"
                     /locus_tag="ACUBV3_000580"
     CDS             18122..19054
                     /gene="yidC"
                     /locus_tag="ACUBV3_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HHU3847110.1"
                     /translation="MKKTLKRILFSSLSLSILLLLTGCVSVDKAGKPYGVIWNTLGVP
                     MANLITYFAQHQGLGFGVAIIIVTIIVRVIILPLGLYQSWKASYQAEKMAYFKPLFEP
                     INERLRNAKTQEEKLAAQTELMTAQRENGLSMFGGIGCLPLLIQMPFFSAIFFAARYT
                     PGVSSATFLGLNLGQKSLTLTVIIAILYFVQSWLSMQGVPDEQRQQMKTMMYVMPIAM
                     VFMSISLPASVALYWFIGGIFSIIQQLVTTYVLKPKLRRKVEEEYTKNPPKAYKSNNA
                     RKDVTSSTKTTESNQAIITSKKTNRNAGKQKRRG"
     gene            complement(19253..19918)
                     /locus_tag="ACUBV3_000581"
     CDS             complement(19253..19918)
                     /locus_tag="ACUBV3_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000611496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HHU3847111.1"
                     /translation="MINWDAIFNLELAVKAFPSVIQGLPYTIGLSLVGFILGAIVGFF
                     VALMKMSHLRLLRYLANIHISLMRGIPLMVLLFLIYFGLPFIGIQLDAVTASIVGFTM
                     MSSAYISEIIRAALLAVDHGQWEAARALGLKTPTIYRGIIIPQATRIALPSLSNVLLD
                     MVKGSSLTAMITVPDIFNNAKIVGGTYSDYMTAYILVALIYWVICTLYAIIQDWWEKR
                     LLY"
     gene            complement(19942..20799)
                     /locus_tag="ACUBV3_000582"
     CDS             complement(19942..20799)
                     /locus_tag="ACUBV3_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HHU3847112.1"
                     /translation="MKKTILLGLVGLSAMTLAACSNGQSSKETTWDNIKKDGVLKVAT
                     PATLYPTSYYDDHKKLTGYEIDMMKAIAKKLKIKVKFVEVGVAESFTSVDSGKVDVAV
                     NNFDTTPERLKKYNFSQPYKYSVGGMIVRADGSSKITAKDLSDWKGKKAGGGAGTQYM
                     KIAKQQGAEPVIYDNVTNDVYLRDVSTGRTDFIPNDYYTQVIAVKYVTKQYPDIKVKM
                     GDVKYNPTEQGIVMSKKDKSLKTKIDAAIKDMKKDGSLKKISEKYYAGQDLTKEPAGT
                     DKIPVIKVK"
     gene            complement(20960..22420)
                     /locus_tag="ACUBV3_000583"
     CDS             complement(20960..22420)
                     /locus_tag="ACUBV3_000583"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HHU3847113.1"
                     /translation="MVFKDATAMAQAIKQHKVSSQELVEQAIYKIEEQNVSVNAVVSK
                     QYNEARQAAKYANASNAPFAGVPILLKDLGQNQKGQLSTSGSQLFKHYYAKQTDYLVQ
                     SFEKLGFIILGRTNTPEFGFKNISDSQLHGNVNLPFDHSRNAGGSSGGAAAAVSSGMV
                     PIAGASDGGGSIRIPASFNGLIGLKPSRGRIPVGPSSYRGWQGASSHFALTKSVRDTK
                     RLLYYLQSYQVESPFPLKKLSKESLFEFSVSKPLKIAVLMDSPLKTKVSSEAKAAIKE
                     AADFLSQKGNHLELVEQPLDGIHSMKTYCMMNSVETAAMFDGIEKSLGRSMEFSDMEL
                     MTWAMYQSGQRVLAKDYSKLLDSWDQFAATMARFHENYDLILTAATNQPAPFHGQFDL
                     DETLQKQLRHMREFSVSEQQDLIWKMFEDSMAWTPFTHQPNLTGQPSLAIPTHLTKEG
                     LPLGVQLTAAKGREDLLLAVAELFEKEKQFKGPVCH"
BASE COUNT         7225 a         4370 c         3504 g         7329 t
ORIGIN      
        1 agaaggacat ccgaactcct tctttctaat aactactata agtatcttag cgatataagt
       61 ttccaacaaa atcctgatca atatcatcaa ttgctgataa ccgaacatcc ttaacaaatt
      121 ttaaatgttt tatctcttta ctaacgctaa caatatcctc tttattaaca taaatgacta
      181 gatatcttaa tctacgagaa tgataatgaa catctccgta tttatataat ttacgagcgt
      241 cacgattata gtaaagataa acgctcattc ctaaacgctc ttgtttattc aataattttt
      301 tcctttctgc gtcatatctg taattactaa gattataaca taattccaac aaataaaaaa
      361 agagttgata aactcaactc ttttaaatct attattgtag attattattt tccataatag
      421 catcaataaa gccataatca agtgtttctt gagcactcat ccaacgatca cgctctgcat
      481 catcatggac tttttcaata gattgaccag aattatcagc taagattttt tctaaagtat
      541 gacgcgtttt taaaagatgc tcagcagcga tagccatatc agattgctgt gtgcctccgc
      601 ctgttccacc cattggttgg tggatcatat attctgcatt cggtaacata aaacgttttc
      661 ctttagcacc actcgaagca ataatggttc ccatcgaagc agccatcccc ataacaatcg
      721 tctgtacgtc cgatttaata aagttcatgg tatccacaat agcaagtcca gccgatactg
      781 aaccacctgg tgtattgaca taaaggtaaa tatcctttgt attatcttgt gcatcgagaa
      841 acaataactg tgcaatgata ctattggcca tattatcctc aacttggcct gtcaacataa
      901 taatacgatc ttttaaaaga cgtgagtaaa tatcataaga gcgttcacca cgacttgttt
      961 gttcaataac tactggaatc atatctttct ccatttcttt ttatcactgt cactattata
     1021 atcatttggt caacaaaggt caaataataa cttttttaag aaaaggcttt ctgagttctt
     1081 cagaaaagcc ttattcatct caaatcttat tttgtaccaa ataagcgatc cccagcatct
     1141 ccgagtccag gaacaatgta gccatgttcg ttgagttttt catccaaagc agcggtataa
     1201 atatctacat ctggatgtgc ttcttgaagt gctgccactc cttctggagc tgctactaaa
     1261 caaacgaatt tgatactagc tgcgccacgt ttttttagag aatctactgc taaaatagca
     1321 gacccacctg tagcaagcat tggatcaact acaaaaatct gacgttgatc aatatcttca
     1381 ggtaacttaa ctaaatattc gactggttgg aaagtctctt catcacgata cataccgata
     1441 tgaccaactt tagcagctgg cacaaggctt aagaaaccat caaccatacc aatacctgca
     1501 cgtaaaattg gaacaattgc taactttttc ccagctaatt gtttttgtac agttgtcgct
     1561 actggtgttt ggatttcaac atcttctaaa ggcaaatcac gagacacttc atagcccatt
     1621 aacatcgcaa tctcatcaac taactcacga aaatctttag tagatgttgt agtgcgacgt
     1681 aagattgaca gtttatgttg aataagtgga tgtgaaatga cttgaaattt tcccattata
     1741 atgaccgctt tctatttaat tttgagcatt aaaaacattc taacgcttgt ttacattcaa
     1801 agtattataa cataaaaacc ctttctatgc gacttttttt atagtctagt cattttttct
     1861 aaagtttgtc actaatcgca aaactgcctc ctcaataaca gacctaggag ctgcgacatt
     1921 gattctagca tgttttttcc cttcttttcc aaaagttaaa ccatcattta atattaattt
     1981 ggcatcatat cgaagttttt cttgaatttc taaatgcgtt aaattataag cagaaaaatc
     2041 taaccaaaca agataagttc cctctggttt catcactttg atatgagttt tttgcgttaa
     2101 ttgctcataa aggtattcta ttgatccttc taactccatt tttagggcct ttaaccattt
     2161 tttccctttt gtaaaggcaa cttctgtagc taataacccc aatgaggaaa tttcttgttg
     2221 attgttagct atttgcctct tcttaaaatc agagcgtaac ttttcatttt caattattgc
     2281 aaaactattt ttagtccctg caatattaaa cgttttagtt gcactagata aaataacact
     2341 aaattcttta aaactagaat caacagtatt gaagctatgg tgtacattat caaataatac
     2401 caaatcttga tgtatttcgt cagaaacgag gataacatta taccgtttac agatatctcc
     2461 tattttttga atttctcctt ttgtccagac acgtccacca ggattatgtg gactacaaaa
     2521 aatataaagc tttacattat tttcaatgat atctttttct aattgtttaa agtcaatctc
     2581 aaaatactga ttattattaa gtaaactatt tgaaactaaa tgacgattat tgtattttat
     2641 agttctagca aaaggcggat agactggggt attaattaga acagcatctc cctcctttgt
     2701 gaaggcttgt atggcaactg aaatcgctgg tataacccca tcaattaata ataatgattt
     2761 tttatcaaat tgataaccat gttcttgcac ttcccaatta gagatagctt ggtataaact
     2821 gtctttagga ctatcatagc caaaaaccat ttggtgtgaa aaatcttcaa tagcctccga
     2881 catttctgga aagataggaa aatccatatc cgcaatccag aggggaagga tttcttgatc
     2941 tttttgaacc gccttccatt ttatggcatt acttgaaaaa cgttctggta aacttgtgaa
     3001 atcatatgta atcataatta ctcctgacta ttctacagct aaaaatatcc cttgtcgcct
     3061 tggtgactat tttaccattt gtttttgata ctatatacta attaacattc taaagataaa
     3121 agtgaccatt ttaaacgatc actttcttta ttattttaat ttatcatttt cataagtatg
     3181 gcttaattct aaattaggaa agctttgctg cctaagggct tcataaacaa tcatacaaac
     3241 agtgtttgaa acattcaagc tacgcacatg ctcatcgttc atgggaattc gtaaggcctt
     3301 ttcggcatgc tcgcgcataa aagtttctgg caatccttta tcttcacgac caaacataaa
     3361 ataatggtct tgatcatcat tataattttc atctgagtaa accttgtcag caaatttgct
     3421 aattaggtga actttccctc gacatgacaa cataaattct tccaaactat catagaaact
     3481 aacatctagc ttatcccagt aatccagtcc cgctcgtttc atttttttat catcaatagg
     3541 aaatcccatt ggtcgtatga tgtgcaaagg agcatttgtt gccgcacaag ttctagcaat
     3601 attaccagta tttgctggaa tctgtggttc aaataaaaca atatgatttc gtccgctatc
     3661 ggaacgatga tttttctgcg ttagtgtttc aatattcatc tcatcatcac tttctagaac
     3721 tcattctgac tcgcaaaaaa accacactgc tcggagtcaa tctcagcaaa caatgtggtt
     3781 tatggtggtt tgttaactta aatcataaca ggtttgattt aaactaacaa atcgttgaca
     3841 ctattatagc acttcagtct acaaagtcaa cctattcatg agtttttggg ttattttttt
     3901 gatagatata atgacaaatc aaacagaata ataagcaaaa acattggaaa attaaaaccg
     3961 taaagaaatt acttaacaaa ctcccgataa agaggatcaa accgcctaaa attgctagaa
     4021 tcggtgaaac taagccggta aatttatttt taatatcttt tttgagataa aggctgagta
     4081 ctaaaacata aagaatgatt aaactagtat tattaaacac cacagcaatt tcactaatat
     4141 cactacccgg caataaatta aattttgtaa ccataaagtg aacaaaaagc caaaaaatag
     4201 ctacagccat caccgtcaaa ctagctggca atgacatttg atacttcaaa tttatgttag
     4261 ccatcctctc acttttgatc catccgcgtt ccgcaaaagc ttgaggtaga cgcatcgtcc
     4321 caagtaaaag tccattggca acacctaata cagataaaat aacaatgaaa gataataaac
     4381 gcccaacact aggaccgaaa ataatgttag ctgcataatt aatagcatca ttaccggttg
     4441 tcataataaa gctagcacct aaaatttgtg tcaaaccata gaaaaatgcc aaatatgata
     4501 ataagataag cgctggacca atgacaaagg caagtggtaa attcttcttg ggattcttga
     4561 cttctggtgc aatactaaca aaaattgtcc aaccatcata tgcaaaatac aatggtacca
     4621 gacctgacac ccaactccat ccaacatttg acggctgaat agctgttaaa tgttgtggaa
     4681 gttgtggagc tttatcagac caaaaagcac caattaaagc aattaggact aaaggaatca
     4741 tttttacaaa ggtcgttaaa gattggaatc cccctgcaat acgcttagca aagatattca
     4801 tgagactaag tgctaaaata taaacagcag ctaaaataat ctgtgattcc aaacttgatg
     4861 attcacctaa agtataaaat gcagctaccc atgaaacgat tgctgttaaa gttggaagat
     4921 acaaaaaaga cgcaaacaag cctaaagttg ctgctaaagc tgggctaaca tatttttcgt
     4981 agtaagaaaa aataccacca ctttcactcg ttctaatagc tagctcagaa attgacaaac
     5041 ttccaaaaac aatcgaaaaa gaacctagta ctaaaattac catgcctaaa aagacatctc
     5101 caccagtaaa tttaaggatg tcatcaactt taaaataaat gcctgaacca atcactacac
     5161 ctacaatcat agcaattgta gtcattaatc cataggtctg tttaatagaa ttatgattct
     5221 ccataatatc tccatttcta ctgtacgctt aaaggttaat tataacctaa tttagggctt
     5281 gtcacaagtt tatctgtgtt atttttcacc agattctgat aagagaatca tattactctt
     5341 atcagaaaga tgaaaacgtt ttttaaccat aattctcgtg catttttact aaaaaaaagg
     5401 tatcatagag tttattattg gaggtaaaaa tgagaataat tgttgtcggc ggaggtaaag
     5461 tcggaaccgc actttgtcgc tcacttgttg ctgaaaagca cgatgttgtt ttaattgaga
     5521 aaaaagaaaa cgtccttaaa cgtgtcacta aacagcatga tattatggga attgttggga
     5581 atggggcaaa ctataaaatt ttagaacaag ccgaagtcaa aaattgtgat atttttatcg
     5641 ctatcactga tcgagatgaa gttaatatga tttcagcagt tcttgccaaa aaaatggggg
     5701 caaaagagac tgtcgttcgc atgcgtaatc ctgagtattc taatccttac ttcaaagata
     5761 aaaactttct tggcttctct tcagttgtca accctgaatt gttagcagca caatatatcg
     5821 ctaataccat agaatttcca aatgcaactt cagttgaaca tttcgccaac gggcgtgtga
     5881 tgttaatgga atttaaaatt ttagaaggaa ataaactctg tcatacttca atgagtcaaa
     5941 ttcgtaaaaa atttggtaat attgttattt gcgcaattga acgcgatggc aaattgatta
     6001 ttcctgatgg cgatgcaact atccaagtca aagataaaat ctttgtaact ggaaaccgta
     6061 ttgaaatgat acttttccat aattacgtta aaaataaagt tgtcaaaaac ttgatggtta
     6121 tcggtgcagg acgcatcgcc tactacctac tcaatatcct taaaaatacc aatacacacg
     6181 tcaaactagt tgaactcaac caagaacaag ctgaatattt tagtcaagaa tttcccaatg
     6241 ttcctgtcgt tcatggggat ggtactgcta aaaatatttt gttagaagaa agcgttacaa
     6301 gttttgacgc agttgctact ttaactggtg ttgatgaaga aaatatcatt acctcaatgt
     6361 tcttagaaag tatcggtatt cctaaaaaca ttaccaaagt taatcgaact agcctactag
     6421 aaatcattga cgataaacaa ctatcaagca ttatcacacc caagcgtatt gccgtagacc
     6481 atgtgatgca ctttgtacgc gggcgtgtaa atgctcagga ttccaaccta gaagctatgc
     6541 atcatattgc taacgatcgc attgaaacct tacaatttga aataaaagaa actagcaaat
     6601 tagcaaatcg tagccttgca tctttaaaat taaagcaaaa tattttaatt gcagcgatta
     6661 ttcgcaataa taaaacgatt ttcccaactg gtgaggatgt tttaacagtg ggtgatcgta
     6721 tcgttgttat caccttatta aaaaatatta ctagaactag tgatatgtta gcgaggtagg
     6781 aatatgaata aaagtatgat tcgtttcctt ctctcaaaac ttttgctgat tgaagctgct
     6841 cttctagcga ttcctctaac tgttggactt atttaccgtg aaccgcaaag tgtcatgatg
     6901 agtatagtca ttaccatgat tattctaatc atccttggat tactgggttc tctctttaaa
     6961 cctaaaaatt atcatatcta cactaaagaa ggcatgttaa ttgttgcact ctgctggata
     7021 ctttggtcct tctttggcgc ccttccattt gttatttcag ggcaaatccc taatattatc
     7081 gatgctttct ttgaagtatc ttctggtttt acgacaacag gagcaactat tcttgatgac
     7141 gtcagtgtct tatcccccgc gctcttattt tggcgtagct ttacccatct tatcggtgga
     7201 atgggggttt tagtatttgc ccttgccatc atggaaaatt ctaaaaatag ccatttagag
     7261 gttatgagag ctgaagttcc tggacctgta tttggtaaag ttgtctcgaa acttaaaaaa
     7321 actgcacaaa ttttgtattt gttatacctt cttatgtttg cagtatttgc agtaatatta
     7381 tattttgcag gtatgccttt ctttgatagt attatcatag caatgggaac tgcaggtaca
     7441 ggtggttttg cagtttacaa tgatagcatt gcccattata atagtcctct gataactaac
     7501 ttagtttcta ttggtatgct tatcttcggg gttaacttta atctttacta tcttctttta
     7561 ctccgaaaaa ttaaagcatt ctttggtgat gaggaactaa aaaactatct ccgcatcgtg
     7621 gcaatagcaa ctttcatgat tgctctaaac gtcatcggta tgtatgataa ttttcgacaa
     7681 ggcctcgaac atattttctt tgaagtatca gctattatta cgacaactgg ttttggcgtg
     7741 actgatatca ctcgttggcc tttgttttca caggttattt tgctattctt gatgtttatc
     7801 ggtggttctg ctggctctac agctgggggc tttaaagtca tgcgtagctt aagcctagca
     7861 aagattgcac gtaaccaagt attgtctaca ctctatccta atagagtcat gtcactacac
     7921 attaataaaa gcgttctaga taaaaataca caacatggcg ttttaaaata tctaactatc
     7981 tatttagcta tcttcatggc actcgttctt gttttaacgc ttgatactaa tgattttttg
     8041 gttgttgtca gtgctgcagc ctcatgtttt aacaacatag gcccactctt aggtagcaat
     8101 gaaactttct cattctttag tccattctca aaactccttc tttcatttgc tatgatagca
     8161 ggacgtttag aaatataccc tgttttattg atgtttattc ctaaaacatg gtcaaaacta
     8221 taattacaaa taataaaacg gatatatcaa tctgatatat ccgttttatt acgtcttaag
     8281 ctaaaatgat cagggacagg atcatttcct ccgtgggcta agggatgaca tcgcaaaata
     8341 cgtgcaatcc ccatcaacac accttttaga ccatgttttt gaatagcttc tatcatatac
     8401 gtagagcaag ttggacgata acgacagcta gctgggaaag ctggagaaat atttttttgg
     8461 taaaagcgaa ccaagaaaat caaaaaagat ttaagcatta agatttatta atagccgcat
     8521 tatgcaactg gctaatttct tttttattta atcgtcgtgc ttccccagga cggagacccg
     8581 ttaaatctaa ggtaccgaat tgtgtacgag atagcttgtc cactaataac ccaacttgtt
     8641 caaacatttt cttaacttga tgattacgac cttcatgaat agttaactca actactgaac
     8701 gatttttttc atgatccact tttatgatat tatagcgagc aggttttgtt ttcttaccat
     8761 caataacaac gccacgcgtt aatggtcgaa ggttttcttt agtagctatt cctttgacac
     8821 gtgctaaata aactttatca atttcattac gaggatgaat cattttatca gtaaaatcac
     8881 catcattagt caaaattaaa agtccagttg tatcccaatc caagcgacca acaggatata
     8941 tccgttcttt gacttgtggc aacaagtcaa taactgtctt acgtccttta tcatctgaga
     9001 cacttgaaat aacaccacgt ggtttattta agagataata taccttctct tcgttgtaaa
     9061 ttggacttcc ttcaatttca accagatcac cagcctttac tttggtcgcc aattcattaa
     9121 ccacttgacc attaatcgtg accattcctt gcttgattaa ttcctcagct tttctacgac
     9181 ttgcaatccc tgcatgcgca atatatttat taattctcat tgttttcctt atcttctgta
     9241 ttaattgaat ccattgaaaa taacgatact tcttcgtcaa ctaattcaat tgaagaggcg
     9301 tcaattaagt catctaactg attaattccc atataatcta agaaataatt agttgtctca
     9361 taaaggttag gacgtcccaa cacttctttc tttccagcct ctttaattaa accaaaagct
     9421 atcagtctcg ttatagctcc actggaattc acgccacgaa tatcatcaat ttcaatccta
     9481 gtaataggtt gtttataagc aataatagat agaacttcca aacttgcccg agatagactc
     9541 tgattaatcg gagccttcgc ataatctctc aacaaagtca tataactatc ctttgttacc
     9601 aatttataag tttgtgctgt ctcaagcaat aatagactag aatcgtcatc ttcttcatat
     9661 cgtttagcca acttttctag ttgctgtatt aatgctgatg gtgtcaaaga caataattct
     9721 gccatttgac gcaaactcag tccatcttca cctgcaacaa aaagaagagc ttctattgag
     9781 cctaaataag tcatttctct tcctttctta agataacttg actaaaatta ctgtcttgtt
     9841 ctactgtgat ttgatgtaat tttatcaact ctaacattgc caaaaataaa gttatcattt
     9901 cagattttgt ttggcaatcc gcaaacactt cttgtaatgt cgttttcttt ttaagattaa
     9961 aatgtctttc aataacgatc atcatatctt caatacggta gtcttctttc tcaattaccg
    10021 tttgactatt tgacaattct ttttgttttt gggacatcat ttgagaaaaa gtcaaaaaaa
    10081 ggtccatcac cgatttatcg tgtagaagaa ttgcatcttc aaagataact tcttgttttg
    10141 gttttgaaaa atatttggca cgttcctgat gctgattagc taactcttca cttagcactt
    10201 taaaacgtct atattcctct aattggctca acaattccat ttctggatca tcttcaatag
    10261 gagttgactc cactacttta ggtaataagt tacggctttt tatcaacatc aactgacttg
    10321 ccataagcat atactcacca gctacttcta gacgcatcgc ctgtaatgtt gctatataag
    10381 caaggtactg ctcaataact tccacaatcg gtacatcgta tatatcaacc tcatatttag
    10441 atacgaggtg taagagcaag tctaggggcc cctcgaaatc ttttaactta atatccatta
    10501 tctataatat ctctctaaag tgataggagt ttttaagcct agaaggcggc tgagctccgt
    10561 cattgttttg ccattttctt tttgctttag aataaattgt tctcgcagaa gttgtgcagt
    10621 tagttgttgc tcattcttct catttaagaa atctgttaat tgattaaaaa accattgtct
    10681 agaataaggc ttaccgttat gctcaaagac taagtcacca tcttcctcta aagattccaa
    10741 aagaaaagga agcaagtttt ctggaagttt taaaatacgc ttcatctgcg atttttcaat
    10801 acttaagata ttaaaagttg tatcaaaatc agccttcttc aaattagcaa tttcagccgg
    10861 agttaaacct aaagataaaa tcaacaaagc aatcaaacga ccactcggat aatctgtatc
    10921 ttgatagaaa tttgataaat ccattatttg agactttgtc tgagtcaaag tgattttttc
    10981 agtctcttgt agtctgtaaa attccttcag cgttcctcta ttatacaaaa agaaaagaaa
    11041 ttgattgact gcagataact ttcgcttacg agctgttaac ttaaattcac ttagagactg
    11101 ttgatacaat gccagttttt cagaatttac atgtccacca attattttat aaaattgctt
    11161 taaatcatag tgataggact tacgcgaatt gagagataat ttcttactct caataaaatt
    11221 attaatatca tttatcattg tttttaggag tgatagtata ttcgtctgta aaatcatgta
    11281 ggaggctatt aatagctttt aaaatcgatt tacgggttat aatgcctcta aaatcatttt
    11341 gatcactgat aacaggtaaa aatggataat ctacaatctt atgcataatt gctgtcagag
    11401 tagctgcctc attaatcggc tcgatttttg tattaaccat ctcaacaata tcagtctgtg
    11461 ccatctccca atctgttaat tgacctttag attggtaagc cataatgtct gagatactaa
    11521 tagtacctac atattttttt tctttagtga taactggtac acgtgaaaaa ccattacttg
    11581 ctaatagtaa cataacgtgg tctgcattgt gcgtatccac aaaaatagca acgtcttcag
    11641 ctggaattag atagtggtcc aaatgactta gcaaaaacga ctcaaattct ttagcaatca
    11701 tcttgaaaat tcctttgtca agttaggata gggttggtgt tggcgagtat aatactctac
    11761 caaaacctta ctatctgtta tagttacaac agcgtaaagc ttctcattta ttggtcctct
    11821 tggctgcaaa acactacctg gattgataaa aatagtttta ccatttttcc aagcatctgc
    11881 acgatgaaga tgaccgtata aacaaatatc cgcgtcttct tgctgagcta ataaatccaa
    11941 tttatcccaa gtaaaattaa tttgatggag atgaccatgg gtttgaacaa taacagtatt
    12001 atctattttt gtaaccaaaa cttccggata acctgaatca taatcacagt ttcctgtgac
    12061 cacatgaatt ccttcccata taggatctga gctaggaagt tcggaatccc cattgtgaaa
    12121 aatagcatcc acttttccca aataatgatt ctttatatct tttacaatgt cacgatctcc
    12181 gtgagaatca ctcattacaa ctaattttct aattgccatt ttggaaatac ctccatcaat
    12241 ttacggacag cttgcccacg atgtgataga ttattttttt cttgtgcgct caattcagct
    12301 gctgtcctac taccttctcc gacaataaag agcggatcat aaccaaagcc attttccccc
    12361 ttaggttccg taccaatata acctggccac tctgcttcaa ctaccaaact ttctttattt
    12421 ggagctgaca cgactaaggt tgtatgaaac tgtgctgaac gtctttcttt gtcaaatacc
    12481 atggctaatt catgcaataa tttggcatta ttacgagcat ctgtcgcgtc aggacctgaa
    12541 aaacgagcag accatacccc tggcaaacca cctaaagcat ctacttttaa tccggaatca
    12601 tctgaaatca ccattttccc agttaatttt gaaatagtct ctgctttaag tcgagcattt
    12661 tcttcaaagg tcataccagt ctcttcaact tctggtaaat ctgggtaatc atttaaattt
    12721 tcaactttga gtcctaattt cccaaataat tctctaaatt ccttagtttt tccttcattg
    12781 tgagtcgcaa ttaatatggt atccccaaaa ttattatcat tctttaaatc aaagaatttt
    12841 tctaaagaag tgccctcttt aggtaagtga actaagaaca actgatcatc ttcactaact
    12901 aagatggccc ctttatgttg agtcactttg atgtgccttg aatactcttg attaatcata
    12961 tcaacgagga atgctaataa ttgaaaatcc gaagtcatct gcacaacgtg aagctgtaga
    13021 ccttcaactt tcaaagttga taatagacga tgaaaaacat cttggatagc ttcgtaagat
    13081 tcatcattac caaaacaagt aaaataatta aaagcttgga aactacctaa aaaccaattg
    13141 ccttcagttt tactttcaaa aattgttttt gtcataatac tatacgctcc acatttattt
    13201 cttgactaag ccattgttct gcaatttctt taaaaccttt tgggctggcg gttgtgtaaa
    13261 agtgatgacc accgtgttta ttttgccaat tatggtttat ctcaaaatag ttcaataaaa
    13321 cagaaatatc acgaacggtt tctgcgccac tatcaattaa tttaacctca gcccccataa
    13381 cattttgaat gatgggacgt aataagggat aatgcgtgca acctaaaatt aaagtatcta
    13441 atttaccaac taatggggac agcgtttcat aaaccacctt tttggctaaa ctagaagaca
    13501 tctgatttga ttccacaatt ggaacaaatt tcggacaagc aagggatacc acagcagtat
    13561 ttggagacaa agcttgaatt ttttgacgat aagcatctga tttaacagtc atgggagtac
    13621 ctataatacc aactttccct gaattagttg atttgatagc tgcgctagct cctggtaaaa
    13681 taacgcctaa aacaggtatg tctagttttt ctttaatttc ttgccaggca actgcagttg
    13741 ctgtattaca agctataaca atcatcttaa catttttagt caataagaaa ttaaccatct
    13801 gccaggtaaa ctctctaatc tgttgagcag gtctaggacc atacggagct ctagcctgat
    13861 ctccaatgaa gattacttcc tcttctggaa gttgacggaa catttcctta acaaccgtta
    13921 aaccacctac accagaatct agaaagccaa taggcctact atccattttt attcctccaa
    13981 tattagtgac taaaagaggc tggacaacag tcctagcctc tctatacaat aaccaatcag
    14041 tcaaatctac acttgaccaa gtttgcccaa atcttgtcta gaaatctgat ggactatcac
    14101 tcactatttc atcactaaac gcttcaatgc taaaacactc ttatttttta gttttgatag
    14161 ctgtttttgc gtgttttaca atattacggt atgtttgttg aactttagct tcactaggtt
    14221 tttgtcccat ttgactcatc atttcacgaa tagcatccgg tgttaaacga gggtgctctc
    14281 caatctcttt ttcaatttgt ttacgagcaa taaaaatgcc tcccacgaga ccaccaaaca
    14341 aagcgacgat aattaataaa atccaaatag ttgttgacat ctattcattc tcctcattta
    14401 tttacatgac tcattatagc aaaaaaagaa agagtttaca aatatatgta aacaaaatca
    14461 aagcctctat cgtccttgtc acgtcaatac actattaaat tttcactaga gcatgattaa
    14521 gcacatagcc gattccttta tcttatccta agtgttttcc ccaacttttg tgtcatccat
    14581 ttccaaatga agagagctat ataaaaatct accaatccat gtagcagaga gcctagccct
    14641 atcaaagaaa atagtgacca taaataagtt gaactactgt tagaagtcat tgtgattata
    14701 taaacagtaa taaattcgag cagaccatga attatgttaa tagctacagc aaataacaat
    14761 gtttgccatt tactcatcaa taaagagggc tttgacttaa tcaagaaagc cgcaattaat
    14821 gcaaatccga tatgtgataa agctctaaga acgattacaa tcggaaaacc agctagtaga
    14881 aatccaagtc ctgtccctaa agctaccaat attgcaactg gaacagatat aaaaatactt
    14941 aaaaataaag gtacatggct tgctaaggtg aaagaagcag gcccaataat tattttagca
    15001 ggcatgatta taggaatcat gattgcaaaa gctactaaaa tagaagtata agaaaccatt
    15061 tgtgttgttt tttttgacat ttttatctcc tttgaagcta ttatacaaaa taatcttaca
    15121 ggtgtaaaga cacctgtaag attatttgtc tatgtataaa attcctgctt tttttagagc
    15181 ctcacatatt tggtcaaaaa ctgactgatc ttgacaagaa atcatatgtg tatgtaaacc
    15241 atctgtcaaa gaggatagca attctgcatt ggaaactttt aatttagcag taaaattatc
    15301 aatgtctttt tggctttgaa tgtttaaggg agcagtcagc atcccataaa tagggtgctc
    15361 tacctccaca ttcataatga ttccttgata ccgcaaaata attttcagtt cttcttctgt
    15421 ctgttctatg ccatgctgac aaaccaatct agctgtgaat tgatgagtac ttagagcact
    15481 ggacataagg tatccttttg gggtagaaat aatatcacat tgttgtgcac gcaataaggc
    15541 aatatctcca acaatcacct gcctactaac actaaaaatc tttgctaatg tgcttgccga
    15601 tatagcttct tttgttcctt ttaaagtcgt taaaatattt tctcgacgct cctgtgcctt
    15661 catataacaa attccttctt tggtgttatt tcacaattaa aaactggcta gaatatctaa
    15721 ccagttagcc tcaatcattt cgtgcaaaaa gacgaagaat atttaagaat agattaataa
    15781 aatcaagata aaggcttagt gccatcgaaa ctgcccaacc atctgcgact tggccgcctt
    15841 ggctattgta gacatattta atcatttggt tatcataagc aatcaatcca gaaaagatga
    15901 tgacacatac gatactaatg atataactca tacccccact accgataaat aaattaacaa
    15961 gacttgcaat taaaatccca attaaagcag ccattaacgc tttacgcata cctgataaat
    16021 cttttttagt cttagctcca atcaaagcca ttgcaaagaa aacagctgca gatgttataa
    16081 aagcttgaag aactgttgtt tgcgtgtacc ttgcgataat gaaacttaaa gtaaacccat
    16141 tcaaagctga ataaatcaaa aataatggta atgcagctgg tgtgtttcga cgcgccgctc
    16201 cactagcaac aaagactaaa accaattcta agatgattgc tccaaaatac aaccctggca
    16261 ttttttgcat gatggcaatc atattttgtg gaaacatata cagcataatc gcagaaacag
    16321 cagctgacaa acctactcct atccctacaa gaccgtaaat cttagcaaaa aattgattca
    16381 gccctgaatc agattgtgtg taaatgacgt tatcattcat atgtattttc ctattctaaa
    16441 ttttaatgat tgtgactacc taagaatttg cgactaaaac ggataggttt cttaaaaata
    16501 cgtttgagtg gacgagaagc ctctcgtact gcccaatact tatcaaccat agtcacaata
    16561 taactttctt tataacgtgg tggtgcaata gtcgctcccc acatgtgttt caaaataata
    16621 tcttcttctc tagcgtttaa atcagttaat tttcgcgcat tacgaaccgc tatccttgga
    16681 tgaacccaag catggctctt gttaaatttc gttacacgcc aatcgtaata aaagaaatca
    16741 tgtagtaaac cacctcgagc agtactactc gcatcccaac caaacttctt agcaatttta
    16801 taacttgtat aactaacatt tatagaatgc tctaagcgag ttgagtgatg atgttggaca
    16861 atagcttcga gtttttggaa acgtggatgg tcaataaggt gaccgaccaa atccatgaag
    16921 tccttatcgt cttgatactt tttcattata atcacccctc tggggatatt ataacatatg
    16981 aacttaaatt aagctgaaaa tgactactcc cgcagctact gctacattaa gactttctgc
    17041 ttgtccaggc atttcaatat gtgctagaac atctgcttct tctgtcatag ttttggaaat
    17101 tccctgtccc tcatttccca tgattaaagc aaaatgatca tatttaggga gatttttata
    17161 atttaccgat tgttcagata aagttgtcgc aatcatagtt atcccttctg ctttacaaaa
    17221 gttaacaaac tgctctattt ctaccctaaa tactggtaaa tgaaagtgac tgccttgcat
    17281 agaacgaagt gttttttggt tatacaaatc agcagatttc tgagataaaa aaacagcatc
    17341 aaaattcgct gcatctgcag ttcgaaccat tgtccctagg tttcctggat cctgcacatc
    17401 ttccaataca agaaaacgcc cttttttaat atctgtccat cttgtctctt gaaaggcaat
    17461 ctcagctaca atcccttgag gagtctgtga gtcagttaat tctttcaaaa catcatctgt
    17521 aacaacaata gcttcctcgg gttttctcaa acgatctatt gccgtttctg tcacaaaaat
    17581 atttaaaaat tgtgcaccat atttttcagc ttcctcaaat aaatgccagc cttcaataag
    17641 atatgaagat ttccgatatt ttttttgtaa caactttttc gtttttttaa tgagattatt
    17701 tgattttgaa gttataatag tagtcataaa cctattatat cagaaaagag gtcaagttat
    17761 gaaaaaagtt catttaattg tctcagggcg cgttcaagga gtcggatttc gctatgcaac
    17821 ttattcctta gctttagaaa taggtgatat ttacggtcgc gtttggaata acgatgatgg
    17881 aactgttgaa attttagcac aatccacaga ttcaaataaa atgacacaat ttattcagaa
    17941 aattcgaaaa ggaccttcaa aatggtctaa agtaacttat gttgatatta aactagataa
    18001 cttcgatgat tttaacgact ttaaaatgag gaattaaaaa tcattctgca gaagtattgt
    18061 ttaattctta aaaaaaaagt aaaatagata ggtatattaa aaattagagg aactaaaaac
    18121 attgaaaaaa acattgaaac gtatcttatt ctctagctta tctttgtcta tattattatt
    18181 attgacagga tgtgttagcg ttgataaggc tggaaaaccc tatggagtta tctggaatac
    18241 tcttggagtg cctatggcca acctcattac ttatttcgct caacaccaag ggcttggttt
    18301 tggtgtcgct atcattattg taaccatcat cgtacgtgtc attatcttac cacttggact
    18361 ttatcagtct tggaaagcta gctatcaagc tgaaaaaatg gcgtacttta agcctttatt
    18421 cgaacctatc aatgaacgac tacgcaatgc taaaacacaa gaagaaaaat tagcagctca
    18481 gacagaatta atgacagctc aacgtgagaa tggccttagc atgtttggcg gtattggctg
    18541 cttgccatta ctcattcaga tgccattttt ctcagctatt ttctttgctg cacgctatac
    18601 tcctggagtc tctagtgcaa ccttcttggg acttaactta ggtcaaaaga gtctgacact
    18661 tactgtcatt atcgccattc tttattttgt acaatcttgg ttgtcaatgc aaggtgttcc
    18721 agatgaacaa cgtcagcaaa tgaaaacaat gatgtatgtg atgccaatag cgatggtatt
    18781 catgtcaatt agtttacctg ctagtgttgc gctttattgg tttattggtg gtattttcag
    18841 tattatccag cagctagtta ccacttatgt acttaagcct aaactacgcc gaaaagtaga
    18901 agaagaatat actaaaaatc ctcctaaagc ctacaaatct aataacgcac gaaaagatgt
    18961 tacaagttca actaaaacca ctgaatctaa tcaagcaatt attacatcta aaaaaacaaa
    19021 tcgtaacgcc ggaaaacaaa aaagacgggg ataaaacaag catactaaat aatggtgtgt
    19081 tgtcctaaac agttaaagat tattatgtaa ttcaagtaaa aaagacttag ctatgtagaa
    19141 catagactaa gtctttttag ttttgcctaa cttttcatat aaaaagcacc ctaaaagtca
    19201 gataaaaatc tagcttttaa agtgcaagta atctagctaa cagactacct tattaataaa
    19261 gtaatcgttt ttcccaccag tcttgaataa ttgcataaag cgtacaaatt acccaataga
    19321 taagggctac taagatatag gcggtcatat agtcggagta ggttccccca actattttag
    19381 cattattaaa gatatcaggt accgtaatca tcgcagtcaa tgatgaaccc ttgaccatat
    19441 ctagcaaaac attacttaaa gaagggagtg caattcttgt tgcttgcgga ataataatgc
    19501 ctctataaat cgtcggtgtt tttaacccta aagctctagc agcttcccac tgaccatgat
    19561 caacagctaa aagagctgct ctgataattt ctgatatata tgcactagac atcatggtaa
    19621 aacctacgat agaagcagtc acagcatcta actgtatccc tataaaaggc aaaccaaagt
    19681 agataagaaa gagtagaacc attaaaggaa tcccacgcat taaggaaata tgaatattag
    19741 ctagatacct cagtaagcgg agatgagaca tcttcattag agctacaaag aatcctacga
    19801 tagctcctaa aataaaacca accaaggata atccaatggt atatggtaac ccctgaataa
    19861 cacttggaaa agccttaaca gctagttcca aattaaaaat ggcatcccag tttatcactt
    19921 atctactcct ctcgaaatat tttatttaac tttaattact ggaattttat ctgttccagc
    19981 aggttctttt gtgagatctt gaccagcata atatttttca gagatttttt tcaaagaacc
    20041 gtctttcttc atatccttga tagctgcatc aatttttgtt ttgaggctct tatctttttt
    20101 actcatcaca ataccttgct cagtcggatt atatttcaca tcacccattt ttactttgat
    20161 atctggatat tgttttgtta catattttac agcaatgact tgtgtgtagt aatcattcgg
    20221 aataaaatct gtacgtccag ttgatacatc tcgaaggtaa acgtcattag ttacgttgtc
    20281 ataaataaca ggttcagcgc cttgctgttt tgcaattttc atatattgcg ttccagcgcc
    20341 accaccagct tttttacctt tccagtcaga taaatctttt gctgtgatct ttgaagagcc
    20401 atctgcacgt acaatcatac ctccaacaga atatttataa ggttgagaga aattgtattt
    20461 tttcaaacgc tcgggagttg tgtcgaagtt attgacagca acatccacct tgccactatc
    20521 tacagaagta aatgattcag caactccaac ttcaacaaac ttaactttta tcttaagttt
    20581 tttagcgatc gctttcatca tatcaatctc ataaccagtc aattttttat ggtcatcata
    20641 ataacttgtt ggataaagcg ttgcaggggt agcaacctta aggacaccat cttttttaat
    20701 attgtcccat gttgtttctt tgctagattg accatttgag caagctgcta atgtcattgc
    20761 agataaaccg actaaaccaa gtaaaattgt ctttttcata agtactcctg tagattaaat
    20821 tatattaaaa tggtttggcg tactctatcg aaaattaaat ttaatgaaaa ttaatacata
    20881 aatttacgat aataatacaa ccattgttaa gagtaccata ttgtatccgt tttcgcaaaa
    20941 atattgttta cttttcaaat taatggcaaa caggcccttt gaattgcttc tctttttcaa
    21001 acaactctgc aacagcaagc aataaatctt ctctcccttt agccgctgtc aattgtacac
    21061 ctaaaggtag cccctcttta gttagatgag ttggtatggc taaagaaggt tgcccagtca
    21121 agtttggctg atgtgtaaaa ggtgtccagg ccattgagtc ttcaaacatt ttccaaatta
    21181 aatcctgttg ttcacttact gagaattctc tcatatgcct caattgtttc tggagtgtct
    21241 cgtctaaatc aaattgtcca tgaaaaggag ctggttgatt agttgctgct gttaaaatta
    21301 aatcataatt ttcatgaaac ctcgccatgg tggctgcaaa ttgatcccat gaatctaaga
    21361 gtttactgta atctttagcc aatacccttt gtccactctg atacatagcc caggtcatta
    21421 attccatatc gctaaattcc attgaacgcc ctaaactctt ttcgatgcca tcaaacattg
    21481 ctgctgtttc aacgctattc atcatacaat aagttttcat agaatgaata ccatctaaag
    21541 gttgttctac taattctaaa tgattccctt tttgagacag aaaatctgcg gcttctttaa
    21601 tagctgcttt agcctcactt gatacctttg tttttaaggg agaatccatc aatactgcta
    21661 tttttagggg tttactaacg ctaaactcga aaagactctc tttgctgagt tttttcaggg
    21721 gaaatggact ttctacttga tatgattgta aataatataa tagtcgtttt gtatctctaa
    21781 ctgactttgt taaagcaaaa tgactagaag ccccttgcca acctctatag gatgaaggtc
    21841 caacaggtat acgtcctcga ctcggtttta aaccaatcaa accattaaaa cttgctggaa
    21901 ttctgattga ccctcctcca tcactagctc ctgcaattgg aaccattcct gaactaacag
    21961 ctgcggctgc accaccacta gagcctccag catttcttga atggtcaaat ggaagattaa
    22021 cattcccgtg aagttgacta tcactaatat tcttaaaacc aaattcaggg gtatttgttc
    22081 ttcctaaaat aataaaccct aatttttcaa aggactgcac taaatagtct gtttgtttag
    22141 cataatagtg tttaaataat tgtgaacctg aggttgagag ctgtcctttt tgattctgtc
    22201 ccaaatcttt cagaagaata ggaactcctg caaaaggagc atttgatgca tttgcgtatt
    22261 tggctgcttg acgcgcctca ttatattgct tgctgacaac agcattaacg cttacattct
    22321 gttcctcgat cttgtagatt gcttgctcta ctaattcttg cgacgaaacc ttatgctgtt
    22381 ttatcgcttg tgccatagcg gtagcatctt taaaaaccat ataaacac
//
LOCUS       DBJYSH010000018        21445 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.018, whole genome shotgun sequence.
ACCESSION   DBJYSH010000018 DBJYSH010000000
VERSION     DBJYSH010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 21445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21445
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.018"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            114..559
                     /locus_tag="ACUBV3_000584"
                     /pseudo
     CDS             114..559
                     /locus_tag="ACUBV3_000584"
                     /inference="COORDINATES: protein motif:HMM:NF012798.6"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(695..765)
                     /locus_tag="ACUBV3_000585"
     tRNA            complement(695..765)
                     /locus_tag="ACUBV3_000585"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(731..733),aa:Thr,seq:ggt)
     gene            complement(836..1141)
                     /locus_tag="ACUBV3_000586"
     CDS             complement(836..1141)
                     /locus_tag="ACUBV3_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HHU3847114.1"
                     /translation="MFPNLLSLISYHEQQIASRRLSHHLGLEQSIVLYAMAKEETISN
                     ERCPHPKLINVLEGKLEIILGDQKHILEKDSLISLSPQEIHHLRAIENSKFLQIELD"
     gene            complement(1143..1481)
                     /locus_tag="ACUBV3_000587"
     CDS             complement(1143..1481)
                     /locus_tag="ACUBV3_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HHU3847115.1"
                     /translation="MSYIEKIEANKVLDLKQIVPIEEEQMLSKTLVQRKDLGMTVFSL
                     DKGQEIGRHSSPGDAMVNILSGLAQITIDQDKFDVAAGECIVMPANIPHSLYAKEAFQ
                     MLLVVVKPEV"
     gene            complement(1502..2257)
                     /locus_tag="ACUBV3_000588"
     CDS             complement(1502..2257)
                     /locus_tag="ACUBV3_000588"
                     /EC_number="2.1.-.-"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000018433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HHU3847116.1"
                     /translation="MSHKEIGHQFLARLGKKRLRPGGVQATNWLIKKGHFSADSRVLE
                     VACNQCTSSIDIAKAFSCHITALDLDSKVIEKAKENVKKAQLEEFIEVIQGNALKLPF
                     PDNSFDIVINEAMLTMLSNSAKEKAIKEYLRVLKPGGRLLTHDVSYQDEDTAKLIDQL
                     RHTINVNVAPLKIDDWQNLFLQNGFSSSEYQFGKMSLMSPLGMIKDEGLFNTIKIISR
                     GLKKENRSQFLKMKHFFTKTGKNLCYIAVASTK"
     gene            2457..4517
                     /locus_tag="ACUBV3_000589"
     CDS             2457..4517
                     /locus_tag="ACUBV3_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HHU3847117.1"
                     /translation="MNKRTFLLLQQLAQSRLATLEQLATQFKTSTRTIRKDIDMINDC
                     LIKAGADLIYFGNEGEVCSDKAKNHLNDIPKMISLTDYRLSREERLILESYFLLVSLT
                     PITIKGLSRKMYLSRNTVVNDLPSLRSFLTQNELILKSDKQGLIIEGSESQKRLLLMH
                     HYISLSDNDFLREYLFPSEDDPSLLISVVRKAEKQNQVVFRDYSFQYVQTYLNVLCSR
                     SANNLMIETEGMFHFSAFSLIISQSILEDLSQKVRFPLSLKEVEFLAFLIGNLTFVKK
                     SSPIDDSFKIQFLTRQFIKQISGDIAYDLTQDISLLENLSKHMETIFYNQPLQAPDSS
                     LLTDIIESNKSIVTAIKKHLSIFHILKSKVFEPNDIYYIAIHICGAVERQRLEIAHFK
                     VLLACHAGIGTSMLLKQRLKEHFNFDIIDVVTIHEAEEEQHRADFIITTVPLQKTNNE
                     TIVVSASLTETDIIRINTLMRKISKEGNVEENPETSQALFKDLLPILKKIGCEQKDLV
                     MIRQVINRHCQKSMSPFEESMAHQVIQLSDLLTPNYIDLQGKASSWEESIRQSARLLR
                     EESLIEESYVEAMIANVLSYGPYILVAKGVAVAHAGIDDGVNGLGMSLYRLDKGVNFG
                     NPELDPIRYIICLCVTDYGKHVTAFNTLLNLFQDSSMREKLDRVDTAKDCYCLIKKYE
                     EKEN"
     gene            4632..5081
                     /locus_tag="ACUBV3_000590"
     CDS             4632..5081
                     /locus_tag="ACUBV3_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000570642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HHU3847118.1"
                     /translation="MIEKLIYNDLISLNHRYRDQTNLLEVLSQYLLQKGYVKTEFSKA
                     ILQREKDYPTGLQLENMAVAIPHTYSEYVLKPFIYINKLKEPISFIQMGTEDEIVMAR
                     YVIVLGISNPKDQAGLLAELMTLFSNPKIVQQLEMAQTKEALKNIFN"
     gene            5100..5378
                     /locus_tag="ACUBV3_000591"
     CDS             5100..5378
                     /locus_tag="ACUBV3_000591"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000734008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HHU3847119.1"
                     /translation="MKKLIVACGSGVATSQTVASKVDRLLKEAGISCDIEAVDMKSLE
                     QHLKTADAYISIVKAKKDYGVPVFNGVAFLTGMGQKQELEKIISFVKG"
     gene            5390..6727
                     /locus_tag="ACUBV3_000592"
     CDS             5390..6727
                     /locus_tag="ACUBV3_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HHU3847120.1"
                     /translation="MDVLQTVINYILDLGAAVFVPFLMLIIGLCMKMKFRDAFTSALI
                     LGIAFTGMGILVNYIMTSMGAAANDLTKHTGLSLPAVDIGWPGAASISWAWPYAFLVF
                     PLQLAINFVMLLLNQTKTLNVDLWNVWNKIFTAVIVTYFTNNVIFGFIAAAIIIILEL
                     KLGDVFAPEVERLTGVPGVTVPHFICLIAVILHPIDELLKKIPVLNKDFDADTLKEKI
                     GVFGENSVMGAIIGFVLGIASGNGIKFALTLAVQAATALTLFPMVSKLFSQALSPISE
                     AVSDFMREKFEDREVYIGLDWPILGGRNELWVAVIFTIPFLLIGSIILPGNIVLPFAG
                     IINLSFVVGALLLTNGNVIRMIIHGLISTPLFLYGATYFTPYMTRLAKETGTLKQKGL
                     ISWATFEGPDIRYLLATVFQGKVLSVILLIIWLALYVWLLKERKKYNTNLIVE"
     gene            6750..7580
                     /gene="rhaD"
                     /locus_tag="ACUBV3_000593"
     CDS             6750..7580
                     /gene="rhaD"
                     /locus_tag="ACUBV3_000593"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HHU3847121.1"
                     /translation="MTYPDYINELLEVTYDMWQHGWDEYNGGNVSYRLTKEEIGTLRH
                     DLEDTLFTYNDSQEEVDVLSVPDNMQEEYLLITASGSHFRTLKNQPEIDTGIIRITST
                     GYQVIAGFNSGKKPTSEIFMHILAHSARVSQNPNHRVVIHNHATNIVIYSLLEEITSR
                     RLTIDLWSVLTESIVVFPDGIAVLPWEVPGTYQIGLDTAHELKKHRLVVWAKHGVLST
                     GVSYQDCFGLIETADKAANIALKLRSTSGKSIQDNNILTTDDLEKVCQKLQVKGRYLL
                     "
     gene            7715..8179
                     /locus_tag="ACUBV3_000594"
     CDS             7715..8179
                     /locus_tag="ACUBV3_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000888633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HHU3847122.1"
                     /translation="MLEDKVIVFQKSHLDSSEEALDYVSHLLLQSGAVKESYPNAIQE
                     REKAFPTGLMFSDYGVAIPHTDIEHVNYSQMAIVSLKNPVVFQQMGNGEDVEVNIIFM
                     LALKEAHQQVDLLSQLIELFQNKVLLKTLLALGNERSAIDQALGLLKAQGIE"
     gene            8192..9523
                     /locus_tag="ACUBV3_000595"
     CDS             8192..9523
                     /locus_tag="ACUBV3_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HHU3847123.1"
                     /translation="MLDILQKFVNLGAIVVLPILIFIFGILLGTKPRKAFISGVTVGI
                     GFVGLNMVVDLLGNSLGPAAQAMVKRFGLHLTTIDVGWPAASAISYGTLLGSLAIPIG
                     VGINLLLLFLGMTKTLMVDMWNFWHAAFVASLVYAVTHNFALGLYATVVYMVMIYLLG
                     DIIAPMIKTFYGFPNITFPHGTSAPGVIFAIPLNWLFDRIPGFNKIDAKPETIQKRFG
                     VFGDTTVMGLIIGIVMGLLANYNVTNTLQLGIKTAAVMVLMPRMVALLMEGLTPISES
                     ANTFVQKRFPGREVNIGMDSALSVGHPAVLSSSLILIPITILLSVILPGNTTIPFGDL
                     ATIPFAVCLMAAVFGGNIIRTVVGGTIYMAVIMYITSWVAPLVTLSAKAANFDLSGHS
                     RITALVEGGLWPTWLFVFAAKTLPWLILTLILIISLSVMYYLNKVKKEGEI"
     gene            9525..9809
                     /locus_tag="ACUBV3_000596"
     CDS             9525..9809
                     /locus_tag="ACUBV3_000596"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000734775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HHU3847124.1"
                     /translation="MKKLLIMCGSGIATSTVVVGKVKTWLEENHYQDQVRIYQSKIAE
                     EINHIDDYDIVISTTVVPDNVQDKVIMGLPLLTGMGVDAVWSELEAQIKD"
     gene            complement(10094..10924)
                     /locus_tag="ACUBV3_000597"
     CDS             complement(10094..10924)
                     /locus_tag="ACUBV3_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HHU3847125.1"
                     /translation="MNHKELHLFNNLSYIDLYPIQFGKEACAPLHSFGPSLKEHYLFH
                     YIVSGRGTFYNTEEETAHKLEAGQGFLIPPHLVCSYEADKADPWTYIWIEFDGLKSDY
                     FLKQAGLDHKHLIFSQEKNSFDSPVYTEAINILASHNQRSAKVIAHLYLFIDALISQS
                     INHKVIPHNETKDFYIREAINFIERNFQFPITVEEIAKSCNLNRHYFCRLFREQTNSS
                     PQQFLIQYRLSKACALLKNRNLNLSDIAEQIGYSNQFNFSAAFKRQYGISPRQWRKTL
                     "
     gene            11163..12440
                     /locus_tag="ACUBV3_000598"
     CDS             11163..12440
                     /locus_tag="ACUBV3_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HHU3847126.1"
                     /translation="MKKMGYYMGLAAACLALLAGCSNNSNKESKASDNKSGTTEISYA
                     IWDSAQEPGIRKIADKFEEKNPKIKVKIQVVSWDAYWTMLEAGATGGSLPDTFWMHSN
                     EIYRYGANKMLLSLDDYIKKSKEVKLSNYPKGLTKIYNIKGEQYAIPKDYDTIGLWYN
                     KKMFDAAGVSYPDETWDWNKLKEAAKKLTKPDGSQYGFLAPLHNQEGYYNFVYQNGGT
                     IITKDKKSGYDDPKTVEALKYYLSFVKENLSPKVTEDKKRAEVMQNGQAAMGYFGSWN
                     LNSFASNDYMKENFDVAVLPKANDGKRSTIFNGLGNAISAKTKHPQAAWKWVEYLSSK
                     EAQDMQAKLGVAISAYKGSDKVWVDSNKTFAIKHFVEMIDYAQIRPYSNATTKWEDKV
                     YEELKPAYLGKESLEKAVKKITKTMNRALEREK"
     gene            12525..13415
                     /locus_tag="ACUBV3_000599"
     CDS             12525..13415
                     /locus_tag="ACUBV3_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HHU3847127.1"
                     /translation="MKNKKKWNEYLWGWALVAPTIIGLMVLNIIPIFQTLKMSFFKSG
                     DFGQNDIFVGLDNYKRLIEDEQVWQATWNTFKYTLLVVPLTVILALVLATLLNSKIKG
                     KHVYRTIFFLPMVAAPAAVTMVWKWLYNTDFGLINFLLRKIGLNGLNWIEDPNIALYS
                     IALIGIWSTVGYSMILILAGLQEIPKDFYESATIDGASAVKQFFSITLPLISPTLFFV
                     VVTSIIQAMQVFDAIYLMESVTSPAYNKTVSLVYLFYNNSFKYADQGYGSTIVMLLLV
                     IILIITLIQMKVQKKWVYYS"
     gene            13429..14262
                     /locus_tag="ACUBV3_000600"
     CDS             13429..14262
                     /locus_tag="ACUBV3_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HHU3847128.1"
                     /translation="MYKTKKKFPYLWAHVFLIFVALGMIIPFIWMVLTSFKTITESTQ
                     LNPFHFFPSKWQVSNYTEVIRTNNFPILYFNTIMMMLWRIVSSVLFSAMAAYAFARLK
                     FPGRNLLFGLVLFQMMVPPQLFVIPQYLMVNQLNMRNTIFALVFPGLVSAFGTFLLRQ
                     FFIGLPDELEEAAKLDGCNIGQTFFNIMLPLAKPGLIALGIFTALFAFKDLLWPLIIN
                     SESDAATLSSALSKIQGAYAVNYPQLMAASVLAIWPMVVLYIIFQKQFIQGISTSGGK
                     L"
     gene            14272..16473
                     /locus_tag="ACUBV3_000601"
     CDS             14272..16473
                     /locus_tag="ACUBV3_000601"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004557 - alpha-galactosidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HHU3847129.1"
                     /translation="MPINFREKEKEFHLYNDSLSYVICVLPNGHIGNLYYGKRLSENM
                     SYAYLLENKYRPLTAYVYEDDDCFSLQHTRQEYASYGSGDMILPAFEISHEDGSHLSH
                     FVFYDYAISKGKPQLSGLPNTYVESDEEATSLELYLKDHNSETELCLTYTIYESRAVI
                     TRNAKFTQRGEKELCLKRALSLCLDLPDKDYEWLHLDGAWGRERYLQSSLLHQGSQSI
                     YSLKGASSAEHNPFIALKRPDANEHEGEVMGFSLVYSGNFLAQVDVTPFHQTRVSMGI
                     HPLGFEWYLKSGESFQTPEVVMVYSHLGLNGMSQIYHDLFSQRLARGEWRDRERPILL
                     NNWEAMTFNFNENSILSLAEVAAKLGVELFVMDDGWFGQRNNEKAGLGDWRVNQKKLP
                     NGLLGISQRIHQLGMLFGLWIEPEMVNKDSDLYREHPDWILHHPKHSQSQGRHQYTLD
                     LSREEVYQNIYQQLHDLLANHGIDYIKWDMNRYMTEAYSYHATKEHQGAIFHRYILNL
                     YRLYETLTSEFPHILFESCSSGGARFDPGMLYYAPQTWTSDDTDAYERLKIQYGTSLV
                     YPLITMGNHVSEVPNQQLLRTTPLSTRANVAYFGSFGYEIDLMLLEEEELELIKKEID
                     FYKNYRRVFQFGNFTRLINPFETDDAAWQVISEDGHHVFVGLYLGLLKLNGPSYHRLH
                     LKGLEAEASYRLNNQVYLGSALMNAGLVINEKDFTEGYKDFASAIYHLEKM"
     gene            16563..17735
                     /locus_tag="ACUBV3_000602"
     CDS             16563..17735
                     /locus_tag="ACUBV3_000602"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018369289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HHU3847130.1"
                     /translation="MTEKEIQTAFANVFHNEAESFFFSPGRINLIGEHTDYNGGHVFP
                     AAITLGTYGAARKRNDQLLRFYSSNFEDIGVIEVNLNNLVYNKADNWANYAKGVLKFL
                     QEAGHTIDTGMDIYVSGNIPNGSGLSSSASLELLIGIMAEEIFNLKLDRLDLVKIGKQ
                     TENKFIGVNSGIMDQFAIGMGADKQAIYLDTNSLAYDLVPLELGDNIVVIMNTNKRRE
                     LADSKYNERRAECEKAVEELQIALDIKTLGDLDEATFDEYSYLIKDANRLKRARHAVS
                     ENQRTLKAKEALISGDLLRFGRLINASHVSLEHDYEVTGIELDTLVHTAWEQDGVLGA
                     RMTGAGFGGCAIAIVAKDNVKQFTANVGKIYSEIIGYSPSFYIAEIAGGSHILTCK"
     gene            17745..19229
                     /gene="galT"
                     /locus_tag="ACUBV3_000603"
     CDS             17745..19229
                     /gene="galT"
                     /locus_tag="ACUBV3_000603"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0017103 - UTP:galactose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019388 - galactose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HHU3847131.1"
                     /translation="MMKTLLDKFVDQLIEIGTFEELDRIYLNNRIMALVGEEGLDQQT
                     DAESLIDIKDKLVDIAVKNGACQELLSKKDMLGAQLMDFITPLPSQVNAAFWKTYKEN
                     PKQAIADFYQLSKANDYIKVKANAKNIYYQVTTDYGNLEITINLSKPEKDPKAIAEAK
                     LSKECHYPKCQLCLENEGYQGRIDYPARTNHRVIRYEINKEIWGFQYSPYAYFNEHCI
                     FFYGKHQPMKISSKTFERLLDIVDRFPGYFAGSNADLPIVGGSILTHEHYQGGRHTFP
                     MEVAEIEEDLTFKGFKKVRAGIVKWPISVIRLRSENQQQLIALATKILENWQNYSDES
                     VHILSESSGIPHHTITPIARKKGNSFELDLALRDNQTSTEFPDGMYHPHLDVQHIKKE
                     NIGLIEVMGLAILPPRLKEELGLVERYLLGEVNEVASYHQKWADNLKLVSEITSGNVE
                     QIVKDAVGKVFSRVLEDAGVFKRTPEGKAAFRRFVESVGVIKGE"
     gene            19231..20226
                     /gene="galE"
                     /locus_tag="ACUBV3_000604"
     CDS             19231..20226
                     /gene="galE"
                     /locus_tag="ACUBV3_000604"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HHU3847132.1"
                     /translation="MAILVLGGAGYIGSHMVDYLLTKTDETVVVVDNLVTGHREAIHL
                     EAIFYKGDLADKTFMRQVFVENPSIDTVIHFAAYSLVAESMEQPLKYFNNNTAGMINL
                     LEVMTEFAIKFIVFSSTAATYGIPNDIPIKEITPQRPINPYGESKLMMETIMKWADAA
                     YGVKFVPLRYFNVAGAKPDGSIGEDHKPETHLLPIILQVAQGKREKVMIYGDDYQTSD
                     GTNIRDYVHPFDIAAAHLLAVDYLRKGNPSTAFNLGSSIGFSNFQILEVARRVTGKDI
                     PVEVTKRRIGDPDILIADSSKARDILGWQPEFDDIEKIISSAWAWHSSHPDGYKS"
     gene            20352..21353
                     /locus_tag="ACUBV3_000605"
     CDS             20352..21353
                     /locus_tag="ACUBV3_000605"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HHU3847133.1"
                     /translation="MTIIKKNFGEKLAFLYELKNKNGMKVTLTNFGARIVDIFIPIDE
                     HLRNISLSATSVEDYYQKDIYVGATVAPVAGRISGAKTHIKGKCYHFTENEPNRTLHS
                     GVDTSVEQYWSTEIEDKENKIIFSLFLPDGKNGFPGNVTVIATYQLTEENELKVNYQA
                     QSDCDTIFNPTNHVYFNLTGDFQKSVASHQLKISSQEFVPLGKDHLPTGEILTVEGTD
                     FDFREFALFSKGLNRFSFQTRLVNGFDHPWLLEVGNNAVEVVSPDNKISLIMKTNQPA
                     VVIYTYNHGPTALAACHGAFSLECQSLPNACNLENLGSILLEKGKVFDSHFSYRFVF"
BASE COUNT         6910 a         3310 c         4188 g         7037 t
ORIGIN      
        1 gaagattatc aaaaattatt agcatattta aaaagaatag ctaattattc agactcaata
       61 atcccttttt tcttttcaca atgttacaaa ctggttttag gtttggtgaa gcaatggcaa
      121 taacttggga tgatattgat tttgaaagaa atagtattta tacatatcga agatattctt
      181 caataaaaaa ggactttaca aagcctaaaa caaggacttc aataaggaga gttccagtaa
      241 gtcatgattt aaaagagtta ttgcttgaac taaaatttca acaagaaaga atgctgaaag
      301 ctttgcatat aaaaaataca gatcaattga ttttttatga ctataggttt ggtatgatat
      361 caaataatgc tgttaacaaa ttcttggcaa gctcattgga aaattagaaa tagaatctaa
      421 gatgtcatca acaggtgcta gacatactta tagaagttat ttgttggcta acggagtaga
      481 tatttgggca gttgcaaaat taatggggca taaagatatc aaacaattaa tagaaacata
      541 tggtcatttg cttctgtaag tggaagaaaa ggaaaatgat cagcttttaa aaattcttaa
      601 taataaaggt agtttttaaa atgactatca agaacaaatt aagaacaaaa ataaaagaaa
      661 ccctattata ataaggtttc actcatatat tttctgccac caccgagaat tgaactcgga
      721 actgagcatt accattgctc tattatacca tttaactata gcggctactt ttctatgata
      781 ctataagatt ttgacgaaat caagtatttc tttacttaga aaaatagtcg tttttttagt
      841 ctaattctat ttggagaaat ttactatttt caatagctct taaatggtgt atttcttgtg
      901 gggagaggct aataaggcta tctttttcaa ggatatgttt ctgatcacca aggataattt
      961 caagttttcc ttccaaaaca tttattaatt ttggatgagg acatcgttca ttgctgatag
     1021 tctcttcttt agccatagcg tacaaaacga tactttgttc cagacctagg tgatgactta
     1081 atcgacgact agcgatttgt tgctcgtgat aagaaataag tgatagtaaa tttggaaaca
     1141 tcttacacct caggcttcac aacgacaaga agcatttgaa aggcttcttt agcatagaga
     1201 ctgtgaggaa tgttagcagg cattacaata cattcaccag ctgctacgtc aaatttgtct
     1261 tgatcaatcg tgatctgcgc aagtccacta aggatattta ccatagcgtc tccgggagat
     1321 gagtggcgtc caatttcttg ccccttgtct aatgaaaaga ccgtcattcc taaatctttc
     1381 cgttgaacta aagttttact aagcatttgc tcttcttcaa taggtacgat ttgttttaaa
     1441 tcaagaactt tattggcttc aattttttcg atataagaca taataactcc tttttaatag
     1501 tctattttgt actagcgaca gcaatatagc agagattttt accagtttta gtaaagaaat
     1561 gtttcatttt taaaaattgg gaacggtttt cctttttcag tcctcggctg atgattttaa
     1621 tggtattgaa taaaccttca tctttaatca ttcctaatgg ggacataaga ctcatttttc
     1681 caaattgata ttctgaactt gaaaaaccat tttgtaagaa taaattttgc caatcatcta
     1741 tttttagagg agcaacgtta acatttatag tatgtctaag ttgatcgatc agcttggcag
     1801 tgtcttcatc ttgataagaa acatcatgtg tgagtaaacg tccaccaggt tttaggacac
     1861 gtaagtactc ttttatggct ttctccttag ctgaattact aagcatcgtt agcattgctt
     1921 cgttgataac gatatcaaaa ctattatcag gaaagggcag tttgagtgca tttccctgaa
     1981 taacttcaat aaattcttct aattgagcct ttttgacatt ttctttggct ttttctatta
     2041 ctttactatc tagatcaagc gctgtaatgt gacaggaaaa tgccttagcg atatcgatgg
     2101 aactggtaca ttgattacaa gctacttcta gaacacgact gtcagcacta aagtgtcctt
     2161 ttttgataag ccaattcgtc gcttggacac ctcctggtct taagcgtttt tttcctagtc
     2221 gtgctaagaa ttgatgtcca atttctttat gtgacaataa gatcacctct ttcttttaat
     2281 agtaatatca ttttttctta actataacac atagatgcta agataacaag gagtaatagt
     2341 gattgaatgg tataaatgat gaattttaga aaacgttttc tttgtaatta ggaatgtggt
     2401 atcatgaaaa tgagtggcgc tacgctagac agcatttctt taagcacgag gttactatga
     2461 ataagagaac gtttttgtta ttacaacaac tagcacaatc aaggctagca actttagagc
     2521 aattagcaac tcaatttaaa acttcaacac gtacgattcg aaaagatatt gatatgatta
     2581 acgattgttt gattaaagca ggagctgatt taatttattt tggtaatgag ggtgaagttt
     2641 gctctgacaa agccaaaaat catttgaacg atattcctaa aatgatttct ctcacagact
     2701 ataggttatc tagggaagaa aggttaattt tagaatctta ttttctatta gttagcttga
     2761 ctcctataac catcaagggc ttaagtcgaa aaatgtattt gagtcgtaat actgtggtta
     2821 atgatttacc ttcattacgc tcttttttga cgcaaaacga attaattttg aaatcagata
     2881 aacaaggttt aatcattgaa ggttctgaat cgcagaagcg tttattgttg atgcatcatt
     2941 atatttcact aagtgataac gattttctca gagaatatct atttcctagt gaagatgacc
     3001 catcgttatt aatttcagta gttcgtaaag ctgagaaaca gaatcaggtt gtttttcgcg
     3061 attactcatt tcaatatgtt caaacttatt tgaatgtttt atgttctcgt tctgctaaca
     3121 acttgatgat tgaaacggaa ggaatgtttc atttttctgc attttccttg attattagtc
     3181 aatcaatatt agaggattta tctcaaaaag ttagatttcc cttatcgctt aaggaggttg
     3241 agtttttagc ttttctaatt ggaaatttga cttttgtgaa gaaatcttct ccgattgatg
     3301 atagttttaa aatccaattt ttaactagac aatttatcaa gcaaatatca ggagatattg
     3361 catatgattt gactcaggac attagtttgc tagaaaattt gtctaagcat atggagacga
     3421 ttttttataa ccaaccttta caagctcctg attcgtcatt gctaaccgat atcattgaat
     3481 ccaataaaag cattgtcaca gccattaaaa agcatttgtc tatcttccat attctcaaat
     3541 ctaaggtatt tgaacccaat gatatctatt acattgctat tcatatttgt ggtgctgtgg
     3601 agcgtcagcg tttggaaata gcacatttta aagttttact ggcttgtcat gcgggtatag
     3661 ggacatcaat gttattgaaa caacgtttaa aagagcactt taactttgat attatagatg
     3721 ttgttaccat tcatgaagca gaggaagagc agcatcgagc tgattttatt ataacgacgg
     3781 ttccactcca aaagacaaat aatgaaacga ttgttgtctc tgcttcttta acagaaacag
     3841 atattatcag aatcaataca ttaatgagaa aaatcagtaa ggaaggcaac gtagaagaaa
     3901 atccggaaac cagtcaggct ttatttaaag atttgttgcc aatactaaaa aaaataggtt
     3961 gtgagcaaaa agatttggtt atgattcggc aagttataaa tcgtcactgc cagaaatcta
     4021 tgagcccatt tgaggagtca atggctcatc aggttattca attatcagat ttattaacac
     4081 ctaattatat tgacttgcaa gggaaagcgt cctcgtggga ggagtctata aggcagtcgg
     4141 ctaggctcct acgggaagag tcattaattg aggaatcata tgtagaagct atgattgcaa
     4201 atgtactttc ttatggtcca tatatattgg ttgcaaaggg agtagctgtt gctcatgcag
     4261 gcattgatga cggtgttaat ggtttgggaa tgtcgcttta tcgcttagat aagggagtaa
     4321 attttggcaa tccagaattg gatcccatcc gttatatcat ttgtttatgt gtaacagatt
     4381 acggtaagca tgtgactgct tttaatactc tgcttaattt atttcaggat agtagtatga
     4441 gagaaaagtt ggatagggtt gatactgcta aagattgtta ttgtctgatt aaaaaatacg
     4501 aagaaaagga aaattagtaa gaccaaaata cttcataaaa actgaagtat tttggtcttt
     4561 ttgtgtgaac attttaaatt tataataaag acagtaaaag ctatcttgca gaaggaggag
     4621 aactattgtc tatgattgaa aagttaatct acaatgattt aatttcttta aatcaccgtt
     4681 atcgagatca aacaaattta ctagaagttt tgtctcaata cttattgcaa aaaggctatg
     4741 ttaaaacaga gttttcaaaa gctattttac agcgagaaaa agattatcca acaggactac
     4801 agttagaaaa tatggctgtt gctattcctc acacctattc agagtatgtc cttaagccct
     4861 ttatttatat caataaatta aaagaaccta ttagtttcat acaaatggga acagaggacg
     4921 aaatagtgat ggcacgttac gtcatcgttt tagggatttc aaatcctaaa gatcaagcag
     4981 gtttactagc tgaattaatg acactctttt ctaatcctaa aatagttcaa caactcgaaa
     5041 tggcacaaac caaagaagcc ttaaaaaata tttttaacta attgatggag gtatttatta
     5101 tgaaaaaatt aattgtagct tgtggttcgg gagttgcgac ctcacaaact gttgcatcta
     5161 aagtagaccg tttgttaaaa gaagcaggaa ttagttgcga cattgaggca gtagatatga
     5221 aatctttaga gcaacattta aaaacagctg atgcttatat ttcgattgtt aaagccaaaa
     5281 aagattatgg tgttcctgtc tttaacggcg ttgctttctt aacaggaatg ggtcaaaagc
     5341 aagaattgga aaaaattatt tcattcgtta aaggttgagg agtagcgtta tggatgttct
     5401 acaaactgtt attaattata ttttagattt aggtgctgct gtttttgttc cttttttaat
     5461 gcttattatc ggtttatgca tgaaaatgaa attccgtgat gctttcacat ctgcacttat
     5521 tctcggaata gcttttacag ggatgggaat cctggttaat tacattatga ctagtatggg
     5581 tgccgcagcg aatgatttaa cgaaacatac cggtttaagt ttaccagcag ttgatattgg
     5641 atggcctgga gcagcctcta tcagttgggc ttggccttat gcttttcttg ttttcccatt
     5701 gcagctggct attaattttg tcatgttact cttgaatcag accaaaacac ttaatgtgga
     5761 tttatggaac gtttggaata agatttttac tgctgtaatt gtaacttatt ttaccaataa
     5821 tgttatcttt ggttttattg cagcagctat tatcattatt ttagaattaa aattaggaga
     5881 tgtctttgct ccagaagttg agcgcttaac aggtgttcca ggtgtcacag tcccacattt
     5941 tatttgttta attgctgtaa ttttgcatcc tattgatgaa ctattgaaaa aaattcctgt
     6001 tttaaataaa gattttgatg cagatacact aaaggaaaaa ataggagtgt ttggagaaaa
     6061 ttcagttatg ggagcgatta tagggtttgt cttagggatt gcttctggta atgggataaa
     6121 atttgcgttg accttagctg ttcaagctgc aacagccttg actttatttc ctatggtttc
     6181 aaaattattt tctcaagcgc tttccccaat ttctgaggca gtgtcagatt ttatgagaga
     6241 aaaatttgaa gatcgggaag tttatattgg tttggattgg ccaatactag gtgggcgaaa
     6301 tgaactttgg gtggctgtta tttttaccat tccattctta ttaataggtt caattattct
     6361 accagggaat attgttttac catttgcagg gattatcaat ctttcatttg ttgtaggagc
     6421 tctattgctc acaaatggca atgtaatccg aatgattatc catggactta tttcgactcc
     6481 tttattctta tatggagcaa cttatttcac accatatatg actagacttg ctaaggaaac
     6541 tggtactcta aagcaaaagg gtctaatttc ttgggcgact tttgagggcc cagacattcg
     6601 ataccttttg gctactgttt ttcaaggtaa agtattatct gttattttac ttatcatttg
     6661 gttggcattg tatgtttggc tattgaaaga acgcaaaaaa tataacacga atttgattgt
     6721 ggaataaaag agaagacaga gggaaaaata tgacatatcc agactatatt aatgaacttt
     6781 tggaagtcac gtatgatatg tggcaacatg gttgggacga atataatggc ggtaatgtga
     6841 gttaccgatt aaccaaagaa gaaattggta ctttaagaca tgatttagaa gataccttat
     6901 ttacttataa tgacagtcaa gaagaagtag atgttctatc tgtaccagat aatatgcaag
     6961 aggagtacct tcttatcact gcttctggta gtcattttag aacattgaaa aatcaaccag
     7021 agattgatac aggcatcata aggataactt ctactggcta tcaagttata gcaggcttta
     7081 atagtggaaa gaaaccaaca agcgaaattt ttatgcatat actagcgcat tctgcgaggg
     7141 taagtcaaaa ccctaaccac cgtgttgtta ttcataatca cgctactaat attgtcattt
     7201 attccctatt agaagaaata actagtcgac gtttaacgat agatctttgg tcagttctaa
     7261 cagaatcaat agttgttttt cctgatggca tagcagtttt accatgggaa gttccaggaa
     7321 cttatcaaat agggttagat acagcacacg aattaaagaa acatcggtta gtcgtttggg
     7381 caaagcacgg tgtcctttca acaggagtta gttatcaaga ttgttttggg ttgattgaaa
     7441 cagcagacaa ggcagctaat attgcactta aacttagaag taccagcggt aagtcaattc
     7501 aagataataa tatattgacc acagatgatt tggaaaaagt atgccaaaag ttgcaagtaa
     7561 aagggcgtta cttattataa ttagctcatg aatgatggat aatagtaaca ggagaccaaa
     7621 atgcttcact ttgagtgaag tattttggtc tttttatgaa atcgattcca tattataata
     7681 aaaccaagaa aacctaagta aaggagaggc gctgatgctc gaagataaag tcattgtatt
     7741 tcaaaaatca catttagatt cttcagaaga agcattggat tatgtatccc atttattgct
     7801 acaatctggt gctgttaaag aaagttaccc caacgctatt caggagcgtg aaaaagcatt
     7861 tcccaccggt ctaatgttct cagactatgg tgttgcgatt cctcatactg atattgagca
     7921 tgttaattat tcacagatgg caatagttag tcttaaaaat cctgtagttt ttcagcaaat
     7981 gggtaatgga gaagatgttg aagttaatat tatctttatg ttagctttaa aggaggctca
     8041 tcaacaggtt gatttattgt cgcagttaat tgaacttttt caaaataaag tattacttaa
     8101 gacattatta gctctaggta atgaaagaag tgctattgac caggctcttg gtcttttaaa
     8161 agctcaagga attgagtagg aggtcttttt aatgttggat attttgcaga aattcgttaa
     8221 tttaggagca atagtcgttt tgccaatact gatatttatc tttggtattt tactgggaac
     8281 gaaacctaga aaagcattta tttctggggt gactgttggt attggttttg taggattaaa
     8341 tatggttgtt gatttactag gtaatagttt aggtcccgca gcacaagcta tggttaaacg
     8401 atttggccta catttaacaa cgattgatgt cggatggcca gcagcctcag caatttctta
     8461 tggaaccctt ttaggaagct tagccattcc gattggtgtt ggtattaacc ttttactcct
     8521 atttttagga atgactaaaa cattaatggt agatatgtgg aatttttggc atgcagcttt
     8581 tgtcgcctcg cttgtttacg cagtaactca taattttgct ctaggtttat atgccacagt
     8641 tgtttatatg gttatgattt atcttctagg tgatattatt gcgcctatga tcaaaacatt
     8701 ttatggcttc cctaacatta cttttccgca tggaacctcg gctccagggg taatatttgc
     8761 tattcctttg aactggttgt ttgaccgcat cccaggtttc aataaaatag atgccaaacc
     8821 tgagactatt cagaagcggt ttggagtttt tggtgataca acagtaatgg ggttaatcat
     8881 tggtattgtt atgggacttc tggctaatta taatgtcact aatactcttc agctaggtat
     8941 taagacagca gcagtcatgg ttttaatgcc acgaatggtt gcccttttaa tggaaggatt
     9001 aacgcctatt tcagagtcag caaatacttt tgttcaaaaa cgttttcctg gtcgtgaggt
     9061 caatattgga atggattcag cgttatcagt tggacatcct gctgttttat ctagttcttt
     9121 aattttgatt ccgataacta ttttattatc agtcatttta cctggtaata cgaccattcc
     9181 ttttggcgat ttagcaacca tcccatttgc tgtttgtttg atggcagcag tatttggtgg
     9241 aaatattata cgtacggtcg taggaggtac tatctacatg gcagtgatta tgtatatcac
     9301 ttcatgggtt gctcctttag tgacattatc tgcaaaagca gctaattttg atcttagtgg
     9361 tcattcacga atcacagctc tagtagaagg tggtttatgg ccaacgtggt tatttgtatt
     9421 tgcagccaag acactaccgt ggctgatact aactcttatt ttgatcatta gtttatctgt
     9481 tatgtattat ctcaataagg ttaaaaaaga aggagaaatt taagatgaaa aaattattga
     9541 ttatgtgtgg ctcagggatt gcaacgtcaa cagttgtggt tggaaaagta aaaacatggc
     9601 tcgaagaaaa tcactatcag gatcaagtgc gtatttacca atcaaaaatc gctgaagaaa
     9661 tcaaccatat tgatgattat gatatcgtta tcagtacgac agttgttcca gataatgtcc
     9721 aagataaagt tattatgggt ttaccgctat taactggtat gggggtcgat gctgtttggt
     9781 cagaacttga agcacaaatt aaggattaat aaaagaagag taatgttttt tgtaagtaag
     9841 gctgatacat gattttagcc ttactttttt cttttgagat aacaattgca tcaattagtt
     9901 aaaagaggcc atgtctgagg gaaataagaa gactctttta ctggcaactt ggttgtgcga
     9961 tgcaaaagag tcgattgtta ttaattactt tgattattct attttttgat aggttccttg
    10021 tttattaacc attataatat ggtgatatta tagggttagt gtagtaaatc ttagctgata
    10081 tttaacgttt agtctaaaga gttttacgcc actgtctagg agatattcca tactgacgtt
    10141 tgaaagcagc agagaaatta aattggttag agtagccgat ctgttcggct atatcactga
    10201 ggttcagatt acggtttttt agcaaagcgc atgccttact caagcgatac tgaattaaaa
    10261 attgttgagg cgagctattg gtttgttctc ggaagagacg acaaaagtaa tgcctattta
    10321 aattacagga tttggctatt tcctcaacag taataggaaa ttgaaaatta cgttcaatga
    10381 agttaatggc ttcacggata tagaaatcct ttgtttcatt atgaggaata actttatggt
    10441 taatagactg actaattagg gcatctataa aaagatagag atgcgcaatg acttttgcac
    10501 ttcgctggtt atgacttgct aagatattaa tagcttctgt gtagactgga ctatcaaaag
    10561 aattcttttc ttgcgagaag atcaagtgtt tatggtctaa accagcttgc tttagaaaat
    10621 aatcactttt tagaccatca aattcaatcc agatataggt ccaggggtca gccttgtcag
    10681 cctcgtaact acaaactaag tgaggaggaa tgagaaaacc ttgccccgcc tccaatttgt
    10741 gagcggtttc ttcttcggta ttataaaagg tacctcgacc tgatacgata tagtggaaaa
    10801 gataatgctc ttttaatgag ggtccaaaag aatggagagg agcacatgcc tctttcccaa
    10861 attgaatagg atataaatca atataagaga gattattgaa taaatggagc tctttgtgat
    10921 tcattattaa tttcctcaaa gtttaataca aattaaagcg tttacttaag cgtattataa
    10981 catagtttat atgagaatgt gattatttat atgattaagt gctaatatga ccataaattc
    11041 atataaataa agtaaaacat tagcattcaa atgtatgcgc ttacatgata aaataaactt
    11101 gtttcaatga gttagaaaat aagcttttct aaccaactat attaaataat aggagtgtag
    11161 ctatgaaaaa aatgggttac tatatgggat tagcagcggc ttgccttgca ctattagctg
    11221 gatgttctaa taacagtaat aaagaatcca aagcgtctga taataaatca ggaacaacag
    11281 agatatcata tgctatttgg gattctgctc aagagcctgg aattagaaag atagctgata
    11341 aatttgaaga aaaaaatcct aaaattaagg tgaaaattca agtggtttct tgggatgctt
    11401 attggacaat gcttgaggca ggagcaacag gtggatcatt gccagatact ttctggatgc
    11461 actctaatga aatttatcgt tatggtgcaa ataagatgct tttgtcactt gatgattaca
    11521 ttaaaaagag taaagaagtt aagctcagca attatccaaa agggttaact aaaatctata
    11581 atatcaaagg tgagcaatac gcaataccaa aagattacga taccattggt ttgtggtata
    11641 acaaaaaaat gtttgatgca gcgggggttt catatccaga tgagacatgg gattggaaca
    11701 aattaaaaga agctgctaaa aaactaacta agcctgatgg tagtcaatac ggctttttag
    11761 caccgcttca caatcaagaa ggctactata attttgttta tcaaaatggt ggtaccatta
    11821 ttacaaagga caaaaaatca ggatacgatg accctaaaac agtcgaagcc ttgaaatact
    11881 atttaagttt tgttaaagag aatttatcac caaaggtgac agaggataaa aaacgtgctg
    11941 aagtgatgca aaatggacaa gcagccatgg gctacttcgg ctcatggaac ctcaattctt
    12001 ttgcttcaaa tgattatatg aaagaaaact tcgatgttgc agttctacca aaagcaaatg
    12061 atggtaaacg ttcaacgatt ttcaatggtt tgggtaatgc tatttctgcc aaaacaaaac
    12121 atccacaagc cgcttggaaa tgggttgaat acttaagttc aaaagaagcc caagatatgc
    12181 aagccaaact tggtgtggca atctctgctt ataagggttc agacaaggtt tgggtggatt
    12241 ctaataagac ttttgctata aaacatttcg ttgaaatgat tgattatgca caaattcgac
    12301 catattcaaa tgctacaact aagtgggaag ataaagtcta tgaagaatta aaacctgcat
    12361 acttaggcaa agaatcacta gaaaaagccg ttaagaaaat tactaaaaca atgaatcgag
    12421 cgcttgagcg ggaaaaataa gatactgaaa aaaactctgg agatgggatt taagggaaaa
    12481 tatttccaat ctcgtctcca gtttttaact aatgaggtac aaagatgaaa aataagaaaa
    12541 aatggaatga gtatttgtgg gggtgggcat tggttgctcc tacgattatt ggtttaatgg
    12601 tcttaaacat tattcctatt tttcaaacgt taaagatgag tttttttaag agtggtgatt
    12661 ttggacagaa tgatattttt gtaggacttg ataattataa gaggttaatt gaggatgaac
    12721 aagtttggca agccacatgg aatactttta aatatactct cttagtagtt cccttaactg
    12781 ttattttggc gctagtttta gcaacgcttc tcaactcaaa gattaaaggt aaacatgttt
    12841 atcgtacaat ctttttccta cctatggttg ctgctccagc agcggtcact atggtttgga
    12901 aatggcttta taatacagat tttggcttaa taaactttct tttacgtaaa ataggattaa
    12961 atggtttgaa ttggattgag gaccctaata ttgccctata ctccatcgct ttaattggta
    13021 tttggagcac tgtaggttat agtatgattt tgatcttagc tggcttacaa gaaattccaa
    13081 aagattttta cgaatccgct acgattgatg gtgccagcgc tgttaaacaa tttttctcta
    13141 taacgctacc tttaatctcc cctactctat tctttgtggt agttactagt attattcaag
    13201 caatgcaagt ttttgatgct atttacctaa tggagtctgt tacaagtccg gcctataata
    13261 agactgtttc gttggtctac ctcttttaca acaattcttt taaatatgcc gaccaaggtt
    13321 acggatccac tatcgtaatg ttattacttg ttattatttt gattattacc ttaatccaaa
    13381 tgaaggtaca gaaaaaatgg gtatattata gttagaaagg gtatcaatat gtacaaaaca
    13441 aaaaagaagt ttccttattt gtgggcacat gtgtttttga tttttgtagc tctaggaatg
    13501 atcattcctt ttatttggat ggtcctaact tcctttaaga ccattacaga atcaacacaa
    13561 ctaaatccat ttcatttttt tccaagtaaa tggcaagtgt ccaattacac tgaagtaatc
    13621 agaacaaaca attttcccat tctttatttc aatactatta tgatgatgtt atggcgtatc
    13681 gttagttcag tattatttag tgctatggca gcatatgcat ttgctcgtct taaatttccc
    13741 ggaagaaacc ttttatttgg attagtccta tttcaaatga tggttcctcc acaattattt
    13801 gttatcccac aatatctaat ggttaatcaa ctaaatatgc gaaatacgat tttcgcgttg
    13861 gtgtttccgg ggttggttag tgcctttggt accttcttac ttcgtcagtt ctttatcggg
    13921 ctaccagatg agttagaaga ggctgctaaa ctagatggtt gtaatatcgg gcagactttc
    13981 tttaacatta tgcttcctct agcaaaacca ggtttaatcg ctttaggaat tttcacagct
    14041 ctatttgcct ttaaagacct tttgtggcct ttaattatca attctgaatc agatgcagct
    14101 actctttcaa gtgcattatc aaaaatacaa ggagcttatg ctgttaatta tccacaacta
    14161 atggcagcaa gtgtacttgc tatatggcca atggtagttc tttatattat atttcagaaa
    14221 cagtttatcc aaggaatttc aacttccggt gggaagttat aggagagaag tatgccaatt
    14281 aattttcgtg aaaaagaaaa agaatttcat ctctataatg acagcctcag ttatgttatt
    14341 tgtgtcttgc caaatgggca tattggaaat ctttattatg ggaaacgatt atcagaaaat
    14401 atgtcctatg cttatttatt agaaaataaa tatcgaccac ttacagccta tgtctatgaa
    14461 gatgatgatt gtttttcgtt acaacatacg cgtcaagaat atgcttctta tggcagtgga
    14521 gatatgattt taccagcttt tgaaattagt catgaagatg gtagtcattt atcacatttt
    14581 gttttttatg attatgctat ttcaaagggg aagcctcaat tatctggttt acctaatacc
    14641 tatgtagaat ctgatgagga agcaactagt ctggagcttt atttaaagga tcataatagt
    14701 gaaacagaac tttgtttaac ttataccatc tatgagagta gagctgttat cacaagaaat
    14761 gctaaattta cccaaagagg cgaaaaagag ctttgtttaa aaagagccct tagcctttgc
    14821 ctagacttac ctgataagga ttatgaatgg cttcatttag atggtgcttg gggaagggaa
    14881 cgttacctac aaagttcgct attacatcaa gggagccaat caatttacag tttaaagggt
    14941 gctagtagtg ccgaacataa tccttttata gctttaaaaa gacccgatgc aaatgagcat
    15001 gagggagaag taatgggctt ttccttagta tatagtggaa attttttagc acaggttgat
    15061 gtgacaccat ttcatcaaac tagagttagt atggggattc atccgttagg atttgaatgg
    15121 tatttaaagt caggagaaag tttccaaacc cctgaggttg ttatggttta cagtcattta
    15181 ggattgaatg gcatgagtca aatctatcac gacttattta gccaacgttt ggctagagga
    15241 gaatggcgtg atagagaacg gcctattctt cttaataatt gggaagctat gacatttaat
    15301 tttaatgaaa attccatttt aagcttagct gaagtagctg ctaagcttgg tgtagaactt
    15361 tttgttatgg atgatggctg gtttggtcaa aggaataatg agaaggctgg ccttggagat
    15421 tggcgtgtga atcaaaaaaa acttcccaat ggtttacttg gtattagtca gagaatccat
    15481 cagttaggga tgttatttgg tttgtggatt gaaccagaga tggtcaacaa agatagcgat
    15541 ctctaccgag aacatcctga ttggatttta catcatccta agcattcaca atctcaagga
    15601 cgccaccagt atacactaga tttgtctcgg gaagaggtat atcaaaatat ttatcaacag
    15661 ttacatgatc tgttagctaa tcatggtatt gactatataa aatgggacat gaatcgttat
    15721 atgacggagg cttacagcta tcatgcgact aaagagcatc aaggagcaat ctttcatcgg
    15781 tatatcctaa acctttaccg actatatgag acgttaacca gtgagtttcc acatattcta
    15841 tttgagtcat gctctagtgg tggagcaagg tttgatccag gaatgcttta ttatgctccg
    15901 caaacatgga ctagcgatga tacggatgct tatgaacgtc taaaaattca gtatggtaca
    15961 agtctggttt atcctcttat aactatggga aatcatgttt cggaggttcc aaatcaacaa
    16021 ctcttacgta ccacaccgct ttctacaaga gctaatgtag cttattttgg aagttttgga
    16081 tatgagattg acttaatgct tcttgaagaa gaggaattag agctaatcaa aaaagagatt
    16141 gatttttaca aaaattatcg gagagtcttc caatttggta attttacgcg tctaattaat
    16201 ccgtttgaaa ctgatgatgc tgcttggcaa gtcatctcag aagatggaca tcatgttttt
    16261 gtaggtctct atttaggttt gctaaaacta aatggtcctt cttatcatcg tttacactta
    16321 aaaggtttag aagcagaagc aagttatcgt ttaaataatc aagtctatct aggaagtgcc
    16381 ttgatgaatg ctggcttagt tatcaacgaa aaagacttca cagagggcta taaagatttt
    16441 gcatcagcta tataccattt agaaaagatg taaaaagctc gaactaactt tcacaacaca
    16501 cttgttagtg ctttgttagt agctaacaat agtaaatgta atagaaggag tgaaaaataa
    16561 ttatgactga aaaagaaata cagacagctt ttgcaaatgt ttttcataat gaggcagaga
    16621 gttttttctt ctcaccagga cgtatcaatt taattggaga acatactgat tataatggag
    16681 ggcatgtctt tccagctgct ataacattag gaacatacgg agctgccagg aaaagaaatg
    16741 atcaattatt acgcttttac tcaagtaact ttgaagacat tggtgtgatt gaagtcaatt
    16801 tgaataatct tgtttacaat aaagctgata attgggctaa ttatgctaaa ggtgttctta
    16861 agtttttaca agaagcagga catactatcg ataccgggat ggatatttac gtttctggca
    16921 atatccctaa cggttcaggt ttatcttcat ctgcttcgct tgaattattg attggcatta
    16981 tggcagaaga gatttttaat cttaaattgg atcgtcttga tttagtaaaa atcggcaagc
    17041 agacagaaaa taaatttatt ggtgttaatt ctggtatcat ggatcagttt gctattggaa
    17101 tgggagctga taaacaggcc atctatcttg ataccaacag tttagcatac gatttagttc
    17161 cgcttgaact gggagacaat attgtagtta tcatgaatac caataaacgt agagaattag
    17221 cagattctaa gtataatgag cgccgagcag aatgtgaaaa agcagtggaa gaattacaga
    17281 ttgctcttga cattaaaact ttgggagact tagatgaagc gacatttgat gagtattctt
    17341 atttgataaa agatgctaat cgtctcaaac gagcccggca tgcggtatct gaaaatcaac
    17401 gcactttaaa ggcaaaagaa gctcttatat caggagattt attacgattt ggacgactaa
    17461 tcaatgcttc tcatgtttcg ttagaacatg actatgaagt gactggaata gagttggaca
    17521 cactagttca tactgcgtgg gagcaagatg gcgtgcttgg agctcgaatg actggagcag
    17581 gttttggcgg ttgtgctatt gctatcgtag ctaaagataa tgtaaaacaa tttacagcaa
    17641 atgtcggtaa aatctatagt gaaattattg gttattcacc tagtttttat attgcagaaa
    17701 ttgccggagg tagccatatt cttacttgta aataaggaaa taatatgatg aaaacattat
    17761 tggataaatt tgtagatcag cttattgaaa tagggacctt tgaagaattg gataggattt
    17821 acctgaacaa tcgtattatg gctttagtag gtgaagaagg cttggatcag caaacagatg
    17881 cagagagttt gattgacatc aaagataaac ttgtagatat agccgtgaaa aatggtgctt
    17941 gtcaggaact attatctaag aaagacatgc tcggtgcaca gttgatggat tttataactc
    18001 cattaccaag tcaagttaat gctgcttttt ggaagaccta taaagaaaac cctaagcagg
    18061 ctattgctga tttttatcag ttgagtaaag caaatgatta tatcaaagtt aaagctaatg
    18121 ctaaaaacat ctattatcaa gtgactacag attatgggaa tttagaaatt accatcaacc
    18181 tctcgaagcc tgaaaaagac cctaaagcta ttgcagaagc taagctttct aaagaatgtc
    18241 attacccaaa gtgtcaactt tgtttagaga atgaaggtta tcaaggtcgt attgattatc
    18301 ctgcacgtac taaccatcgt gttattcgat atgagataaa taaagaaatc tggggattcc
    18361 aatattcacc ttacgcttac tttaatgagc actgtatttt cttttatgga aaacaccagc
    18421 ctatgaagat tagttctaag acatttgagc gtctcttaga tattgtcgat aggtttcctg
    18481 gatattttgc aggatcaaat gctgaccttc ctattgtcgg tggctctatt ttaacccatg
    18541 agcattatca aggaggtcgc cacacatttc caatggaagt agcagagata gaggaagacc
    18601 ttactttcaa aggttttaaa aaagtaagag caggtattgt aaagtggcca atatcagtta
    18661 tacgcttgcg aagtgaaaat cagcaacaat taatcgcttt agcaacaaag attctagaga
    18721 attggcagaa ttattcagat gagtctgttc atattttatc tgagagttca ggtattccac
    18781 accatacaat tacacctatt gctcgaaaaa aaggtaatag tttcgagtta gatttggcct
    18841 tacgtgataa tcaaacatca acagaatttc cagatggtat gtatcatcca caccttgatg
    18901 ttcaacatat taagaaagaa aatattggct taatagaagt catgggatta gcgattttgc
    18961 cgccgcgtct caaggaagaa cttggtttag tcgaaaggta tcttttaggg gaagttaacg
    19021 aggttgcctc ctatcaccaa aagtgggcag ataatttaaa attagtaagt gagattacct
    19081 caggaaatgt tgaacagatt gtcaaagatg ctgtaggtaa agtgttttcc cgtgtacttg
    19141 aggatgcagg agtattcaaa cgaactccag aaggtaaagc ggcttttagg agatttgtgg
    19201 agagcgtagg agtcataaag ggagaataaa atggcaattt tagtcttagg tggtgcaggt
    19261 tatatcgggt ctcatatggt agattactta ctgacaaaga cggatgagac agttgttgtt
    19321 gtagataatt tagtgaccgg acatagagaa gcaatacact tagaagcgat tttctacaag
    19381 ggagatttag cggataagac ttttatgaga caggtatttg tagaaaatcc tagtattgac
    19441 actgtaattc actttgcagc ctattctctg gtagcagaat ctatggagca gcctttaaaa
    19501 tattttaata ataatacagc aggcatgatt aatctcttag aagttatgac tgaatttgct
    19561 ataaaattta tcgtattttc ttcaactgca gcaacttatg gtattccaaa cgacatacca
    19621 attaaagaaa taacgcctca acgtcccatt aacccatatg gtgaaagtaa gttgatgatg
    19681 gaaactatta tgaagtgggc tgatgctgct tatggggtta aatttgttcc tttgcgctat
    19741 tttaatgttg caggagcaaa accggatggt tcaattggtg aagatcataa acctgagact
    19801 caccttttac caatcattct tcaagtagca caaggaaaac gcgagaaggt tatgatttac
    19861 ggtgatgatt atcaaacatc agatggaacc aatattcgtg actacgttca tccatttgat
    19921 atagccgctg ctcacttact agcggttgat tatcttcgta aaggcaatcc ttcaactgcg
    19981 tttaatttag gttcctctat aggattctct aatttccaaa ttttagaagt agctcgcaga
    20041 gtgactggta aagacattcc tgttgaagtg acaaaacgtc gtattgggga tccagatatc
    20101 cttatagccg attctagcaa agcccgtgac attttgggtt ggcagccaga gtttgatgat
    20161 attgaaaaga taatctcttc agcatgggct tggcattcga gtcacccaga tggctataaa
    20221 tcctaaagaa taaagcttta accatatgat aagaaattta cttcaagcag attagtattt
    20281 ttggattaga aagagtctta ttagcataat gaatttatca ggtatcatta gtttgaaaga
    20341 agaaaaaata tatgactatt attaagaaaa acttcggaga gaagctggca ttcctctacg
    20401 aattaaaaaa taaaaatgga atgaaggtca ctttgacgaa ttttggtgca cgtatagttg
    20461 atatcttcat tccgattgat gaacacttgc gtaatattag tttatcggct acttctgttg
    20521 aagattatta tcaaaaagat atttatgtgg gggcaacggt ggcccctgtg gcaggtagaa
    20581 tttctggagc taaaacccat atcaaaggaa aatgttatca ttttactgaa aatgaaccta
    20641 ataggacatt acatagtggt gttgatacat ctgtagaaca gtattggtct acagaaatag
    20701 aagataaaga gaataaaatt atcttttctc tctttcttcc ggatggtaaa aatggctttc
    20761 ctgggaatgt gactgtgata gctacttatc agttgacaga agaaaatgaa ctcaaggtca
    20821 actatcaagc acaatctgat tgtgatacca tttttaaccc aacaaatcat gtatatttta
    20881 atttaacagg ggattttcaa aaatccgttg ctagccatca gttaaagatt tcaagccaag
    20941 agtttgtgcc actagggaaa gaccatttac ctactggtga aatactgact gtggaaggta
    21001 cagactttga ttttagagaa tttgcccttt tttcaaaagg tctaaatcgc ttttctttcc
    21061 aaacgagatt agtaaacgga tttgatcatc cgtggctttt agaagtaggc aataatgccg
    21121 ttgaggtagt tagtcctgat aataaaataa gcttaataat gaaaaccaat caaccagctg
    21181 ttgttattta cacctataat catggtccaa cagcactagc agcttgtcat ggagctttta
    21241 gtttagaatg ccaatctctt cctaatgctt gtaatctaga aaacttagga tcaattttgc
    21301 tcgaaaaagg gaaggtattt gatagccact tttcttatcg ttttgtgttt taatatgaca
    21361 tctaaaagat gtgatgccaa aactagaatg gcatagagaa ccatacattt caataggtct
    21421 tccttagata aaactagttg ttttt
//
LOCUS       DBJYSH010000019        21346 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.019, whole genome shotgun sequence.
ACCESSION   DBJYSH010000019 DBJYSH010000000
VERSION     DBJYSH010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 21346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21346
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.019"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            42..1676
                     /locus_tag="ACUBV3_000606"
     CDS             42..1676
                     /locus_tag="ACUBV3_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HHU3847134.1"
                     /translation="MMKKGQVNDTKQSYSLRKYKFGLASVILGSFIMVTSPVFADQTT
                     SVQVNNQTGTSVDANNSSNETSASSVITSNNDSVQASDKVVNSQNTATKDITTPLVET
                     KPMVEKTLPEQGNYVYSKETEVKNTPSKSAPVAFYAKKGDKVFYDQVFNKDNVKWISY
                     KSFCGVRRYAAIESLDPSGGSETKAPTPVTNSGSNNQEKIATQGNYTFSHKVEVKNEA
                     KVASPTQFTLDKGDRIFYDQILTIEGNQWLSYKSFNGVRRFVLLGKASSVEKTEDKEK
                     VSPQPQARITKTGRLTISNETTTGFDILITNIKDDNGIAAVKVPVWTEQGGQDDIKWY
                     TAVTTGDGNYKVAVSFADHKNEKGLYNIHLYYQEASGTLVGVTGTKVTVAGTNSSQEP
                     IENGLAKTGVYNIIGSTEVKNEAKISSQTQFTLEKGDKINYDQVLTADGYQWISYKSY
                     SGVRRYIPVKKLTTSSEKAKDEATKPTSYPNLPKTGTYTFTKTVDVKSQPKVSSPVEF
                     NFQKGEKIHYDQVLVVDGHQWISYKSYSGIRRYIEI"
     gene            1820..3055
                     /locus_tag="ACUBV3_000607"
                     /pseudo
     CDS             1820..3055
                     /locus_tag="ACUBV3_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175197.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyltransferase"
     gene            3186..3929
                     /locus_tag="ACUBV3_000608"
     CDS             3186..3929
                     /locus_tag="ACUBV3_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HHU3847135.1"
                     /translation="MLKSKRKEIILSRLEQNKSVTLDELTSILETSESTVRRDLDELE
                     SEGFLKRVHGGAELPYSLGQELSNQEKSIKNVQKKLDIARQTAKLIAKQDVIFIDAGT
                     TTELLIDFLPHEQLTVVTNSIHHAAKLVDRGIKTIIIGGAVKHSTDASIGQVAINQIR
                     QITVDKAFLGMNGIDEVYLTTPDLEEAAIKEAIINNSQQTFILMDSSKIGQVTFAKVK
                     EINDINLVTNKTDSELMTIIKEKMKVIQV"
     gene            3926..4837
                     /gene="pfkB"
                     /locus_tag="ACUBV3_000609"
     CDS             3926..4837
                     /gene="pfkB"
                     /locus_tag="ACUBV3_000609"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017284863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HHU3847136.1"
                     /translation="MIYTVTLNPSIDFIVRLDTLLLGSVNRMTSDDKYVGGKGINVSR
                     ILKRLKIDNTATGFIGGFTGHFVEDGLVSEGIKTDFVSVNEDTRINVKVKAKIETEIN
                     GGGPRITNEQLHRLEKLLSRLTPEDTVVFAGSAPASLGNKVYNTLIPIAKKTGAEVVC
                     DFEGQTLLDALAYQPLLVKPNNHELADIFGVELEGLPDIEKYAHKILDKGAKNVIVSM
                     AGDGALLVTPEASYFAKPIKGEVKNSVGAGDSMVAGFTGEFVKSKNPVEALKWGVACG
                     TATTFSDDLATAEFIQDIYNKVEVEKL"
     gene            4834..6798
                     /locus_tag="ACUBV3_000610"
     CDS             4834..6798
                     /locus_tag="ACUBV3_000610"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIABC"
                     /protein_id="HHU3847137.1"
                     /translation="MKIQDLLKKEVMIMDLKATSKEAAIDEMITKLVDTGVVTNFAIF
                     KDGIMKREAQTSTGLGDGIAMPHSKNAAVKEATVLFAKSASGVDYEALDGQPTDLFFM
                     IAAPDGANDTHLAALAELSKYLLKEGFADQLRQAKTPDDIIATFDSNSISQETVAPQT
                     VQSTSKGSDYIVAVTACTTGIAHTYMAEEALKKKAAEMGVGIKVETNGASGVGNKLTS
                     SDIARAKGVIIAADKAVEMDRFDGKPLVSRPVADGIKKSEDLINIILDNKAQTYHAKN
                     QNDKQSGESDGKSGLGSAFYKHLMGGVSQMLPFVIGGGIMIAIAFLFDNILGVPKDQL
                     SNLGSYHEIAALFKNIGGAAFAFMLPVLAGYIAYSIAEKPGLVAGFVAGSIASSGLAF
                     GKVPFAEGGKATLALAGVPSGFLGALVGGFLAGGVILLLRKLLSGLPKSLEGIKSILL
                     YPLLGVLITGFLMLLVNIPMAAINTALNTFLQGLSGSSAVLMGLLVGGMMAVDMGGPV
                     NKAAYVFGTGTLAATVANGGSVVMAAVMAGGMVPPLAVFVATLLFKDKFNNEERQSGL
                     TNIVMGLSFITEGAIPFGAADPARAIPSFIVGSALTGALVGLAGIKLMAPHGGIFVIA
                     LTSNPLLYILFILIGAVVSGVLFGLFRKTK"
     gene            6987..7481
                     /locus_tag="ACUBV3_000611"
     CDS             6987..7481
                     /locus_tag="ACUBV3_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2127 domain-containing protein"
                     /protein_id="HHU3847138.1"
                     /translation="MIKWNEKETKLVDTSFSIMLYLKTLFAVGQVVAGVLLYYFKPTQ
                     IKHLLFLLTEGEVHGGIHGFISAHFLKISSYINDSASHLAAIYLLIHGGIKLVALILL
                     HKEKLFAYPLSILVFVGFIIFQLREYFSSHSILMIFLCVFDVIMIILTWLEYQKMKLN
                     FSKR"
     gene            7532..8065
                     /locus_tag="ACUBV3_000612"
     CDS             7532..8065
                     /locus_tag="ACUBV3_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3847139.1"
                     /translation="MTMKTIEEIYKNYPNIPYISPERDLAEINFSKVVPRKNMEETSE
                     GLLPGDIILLWRIQFGTFTTETSFSKYFEYIYGINGKEHLEFLIKNGFVRMESPLDSL
                     DHLSAPLLKLFLKEKNVKGLSKMKRSDLDQAIAIAFTEEELGKLFVVRGLALTEKGLA
                     ALSNNQEVIDRHPKKKF"
     gene            8137..8517
                     /locus_tag="ACUBV3_000613"
     CDS             8137..8517
                     /locus_tag="ACUBV3_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000452064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HHU3847140.1"
                     /translation="MEVIREQEFVNQYHYDARNLEWEEENGTPKTNFEVTFQLANRDE
                     AAKVTSIVAVLQFVIVRDEFVISGVISQMAHIQGRLINEPSEFSQDEVENLAAPLLEI
                     VKRLTYEVTEIALDRPGVTLEFNS"
     gene            8517..9365
                     /locus_tag="ACUBV3_000614"
     CDS             8517..9365
                     /locus_tag="ACUBV3_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HHU3847141.1"
                     /translation="MKLALITDTSAYLPEAIENHEDVYVLDIPIIIDGKTYIEGQNLT
                     LDQYYDKLAASKELPKTSQPSLAELDDLLCQLEKEGYTHVLGLFIAAGISGFWQNIQF
                     LIEEHPNLTIAFPDTKITSAPQGNLVRNALMCSREGMDFDVIVNKIQSQIEKIEGFIV
                     VNDLNHLVKGGRLSNGSAIIGNLLSIKPVLHFNEEGKIVVYEKVRTEKKALKRLAEIV
                     KEMTADGEYDIAIIHSRAQDKAEQLYNLLAKAGLKDDLEIVSFGGVIATHLGEGAVAF
                     GITPKN"
     gene            9550..10758
                     /locus_tag="ACUBV3_000615"
     CDS             9550..10758
                     /locus_tag="ACUBV3_000615"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HHU3847142.1"
                     /translation="MRLNYLPSEFQKALPILKKIKKAGYEAYFVGGSVRDVLLDRPIH
                     DVDIATSSYPEETKQIFKRTVDVGIEHGTVLVLEKGGEYEITTFRTEEVYVDYRRPSQ
                     VNFVRSLEEDLKRRDFTVNAFALNEDGEVIDLFHGLDDLDNHLLRAVGLASERFNEDA
                     LRIMRGLRFSASLNFDIETTTFEAMKKHASLLEKISVERSFIEFDKLLLAPYWRKGML
                     ALIDSHAFNYLPCLKNRELQLSAFLSQLDKDFLFETSEQAWASLILSMEVEHTKTFLK
                     KWKTSTHFQKDVEHIVDVYRIREQMGLTKEHLYRYGKTIIKQAEGIRKARGLMVDFEK
                     IEQLDSELAIHDRHEIVVNGGTLIKKLGIKPGPQMGDIISQIELAIVLGQLINEEEAI
                     LHFVKQYLMD"
     gene            10769..12637
                     /locus_tag="ACUBV3_000616"
     CDS             10769..12637
                     /locus_tag="ACUBV3_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HHU3847143.1"
                     /translation="MSDFLVDGLTKSVGDKTVFSNVSFIIHSLDRIGIIGVNGTGKTT
                     LLDVISGELGFDGDRSPFSSANDYKIAYLKQEPDFDDSQTILDTVLSSDLREMALIKE
                     YELLLNHYEESKQSRLEKVMAEMDSLDAWSIESEVKTVLSKLGITDLQLSVGELSGGL
                     RRRVQLAQVLLNDADLLLLDEPTNHLDIDTIAWLTNFLKNSKKTVLFITHDRYFLDNV
                     ATRIFELDKAQITEYQGNYQDYVRLRAEQDERDAASLHKKKQLYKQELAWMRTQPQAR
                     ATKQQARINRFQNLKNDLHQTSDTSDLEMTFETSRIGKKVINFENVSFSYPDKSILKD
                     FNLLIQNKDRIGIVGDNGVGKSTLLNLIVQDLQPDSGNVSIGETIRVGYFSQQLHNMD
                     GSKRVINYLQEVADEVKTSVGTTSVTELLEQFLFPRSTHGTQIAKLSGGEKKRLYLLK
                     ILIEKPNVLLLDEPTNDLDIATLTVLENFLQGFGGPVITVSHDRYFLDKVANKIIAFE
                     DNDIREFFGNYTDYLDEKAFNEQNNEVISKKESTKTSREKQSRKRMSYFEKQEWATIE
                     DDIMILENTITRIENDMQTCGSDFTRLSDLQKELDAKNEALLEKYDRYEYLSELDT"
     gene            12634..13107
                     /locus_tag="ACUBV3_000617"
     CDS             12634..13107
                     /locus_tag="ACUBV3_000617"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HHU3847144.1"
                     /translation="MIIRPIIKNDDQAVAQLIRQSLRAYDLDKPDTAYSDPHLDHLTS
                     YYEKIEKSGFFVIEERDEIIGCGGFGPLKNLIAEIQKVYIAERFRGKGLATDLVKMIE
                     VEARKIGYRQLYLETASILSRATAVYKHMGYCALSQPIANDQGHTAMDIWMIKDL"
     gene            13174..14913
                     /locus_tag="ACUBV3_000618"
     CDS             13174..14913
                     /locus_tag="ACUBV3_000618"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HHU3847145.1"
                     /translation="MAYIWSYLKRYPNWLWLDLLGAMLFVTVILGMPTALAGMIDNGV
                     TKGDRTGVYLWTFIMFIFVVLGIIGRITMTYASSRLTTTMIRDMRNDMYAKLQEYSHH
                     EYEQIGVSSLVTRMTSDTFVLMQFAEMSLRLGLVTPMVMIFSVVMILITSPSLAWLVA
                     VAMPLLVGVILYVAIKTKPLSERQQTMLDKINQYVRENLTGLRVVRAFARENFQSQKF
                     QVANQRYTDTSTGLFKLTGLTEPLFVQIIIAMIVAIVWFALDPLQRGAIKIGDLVAFI
                     EYSFHALFSFLLFANLFTMYPRMVVSSHRIREVMDMPISINPNTEGVTDTKLKGHLEF
                     DNVTFAYPGETESPVLHDISFKAKPGETIAFIGSTGSGKSSLVNLIPRFYDVTLGKIL
                     VDGVDVRDYNLKSLRQKIGFIPQKALLFTGTIGENLKYGKADATIDDLRQAVDISQAK
                     EFIESHQEAFETHLAEGGSNLSGGQKQRLSIARAVVKDPDLYIFDDSFSALDYKTDAT
                     LRARLKEVTGDSTVLIVAQRVGTIMDADQIIVLDEGEIVGRGTHAQLIENNAIYREIA
                     ESQLKNQNLSEGE"
     gene            14918..16687
                     /locus_tag="ACUBV3_000619"
     CDS             14918..16687
                     /locus_tag="ACUBV3_000619"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HHU3847146.1"
                     /translation="MRKKSIFLRLWSYLTRYKATLFLAIFLKVLSSFMSILEPFILGL
                     AITELTANLVDMAKGVSGAELNVPYIAGILIIYFFRGVFYELGSYGSNYFMTTVVQKS
                     IRDIRHDLNRKINKVPVSYFDKHQFGDMLGRFTSDVETVSNALQQSFLQIINAFLSII
                     LVVVMVLYLNVPLAMIIIACIPVTYFSAQAILKRSQPYFKEQAKILGELNGFVQEKLT
                     GFNIIKLYGREEASSQEFRDITDNLRHVGFKASFISGIMMPVLNSISDFIYLIIAFVG
                     GLQVIAGTLTIGNMQAFVQYVWQISQPVQTITQLAGVLQSAKSSLERIFEVLDEEEEA
                     NQVTEKLSHDLTGQVSFHGVDFHYSPDKPLIRDFNLDVEPGQMIAIVGPTGAGKTTLI
                     NLLMRFYDVSEGAITVDGHDIRHLSRQDFRQQFGMVLQDAWLYEGTIKENLRFGNLEA
                     SDEDIVAAAKAANVDHFIRTLPGGYNMVMNQESSNISLGQKQLLTIARALLADPKILI
                     LDEATSSVDTRLELLIQKAMKKLMEGRTSFVIAHRLSTIQEADNILVLKDGQIIEQGN
                     HQKLLADKGFYYELYNSQFSNSK"
     gene            complement(16728..17237)
                     /locus_tag="ACUBV3_000620"
     CDS             complement(16728..17237)
                     /locus_tag="ACUBV3_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009268 - response to pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HHU3847147.1"
                     /translation="MKWFPLVGGIIALLAGFYLFINPVTVVATLGWFIALFIFIYGIS
                     NLVRYLQADSYHRSPWYLVQSILAIIFGIILLSSSAMSLSNTVMTILAYWLLVSGILR
                     LLGGFQMQKINFPGAKSYKRSAYIALILGIILLVQPTLSAAIIGRFVAFILIAIGASA
                     IYTFTRINY"
     gene            17405..18754
                     /gene="gdhA"
                     /locus_tag="ACUBV3_000621"
     CDS             17405..18754
                     /gene="gdhA"
                     /locus_tag="ACUBV3_000621"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HHU3847148.1"
                     /translation="MTSGKTYVASVLEKVKKQNEHEEEFLQAVEEVFESLVPVFDKYP
                     QYIEENLLERLVEPERVISFRVPWVDDKGQVQVNRGYRVQFSSAIGPYKGGLRFHPTV
                     TQSIVKFLGFEQIFKNSLTGLPIGGGKGGSNFDPKGKSDNEVMRFTQSFMTELQKHIG
                     PDLDVPAGDIGVGGREIGYLYGQYKRLNGYQNGVLTGKGLTYGGSLARTEATGYGAVY
                     FAKEMLAARGQDLTGKVALVSGSGNVAIYATEKLQELGATVVAVSDSSGYVYDPDGID
                     LETLKQIKEVERARIVKYTEKHPKANFTPADQGSIWSIKADLAFPCATQNELDEEDAK
                     LLVENGVLAVTEGANMPSTLGAIKVFQKAGVAFGPAKAANAGGVAVSALEMAQNSSRR
                     AWTFEEVDQELQRIMKTIFVNASEAADEFGDSGNLVLGANIAGFLKVAQAMSAQGIV"
     gene            18824..19234
                     /locus_tag="ACUBV3_000622"
     CDS             18824..19234
                     /locus_tag="ACUBV3_000622"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HHU3847149.1"
                     /translation="MIKPIVRDTFFLQQKSQMASRADVSLAKDLQETLHANQNYCVGM
                     AANMIGSLKRVIIINVGITNLVMFNPVVVAKSDPYETEESCLSLVGCRSTQRYCHITI
                     SYRDINWKEQQIKLTDFPAQICQHELDHLEGILI"
     gene            complement(19271..21343)
                     /locus_tag="ACUBV3_000623"
     CDS             complement(19271..21343)
                     /locus_tag="ACUBV3_000623"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-anchored 5'-nucleotidase"
                     /protein_id="HHU3847150.1"
                     /translation="MKKKIILKSSVLGLVAGTSIMFSSVFADQVGVQVIGVNDFHGAL
                     DNTGTANMPDGKVANAGTAAQLDAYMDDAQKDFKQTNPNGESIRVQAGDMVGASPANS
                     GLLQDEPTVKNFNAMNVEYGTLGNHEFDEGLAEYNRIVTGKAPAPDSNINNITKSYPH
                     EAAKQEIVVANVIDKVNKQIPYNWKPYAIKNIPVNNKSVNVGFIGIVTKDIPNLVLRK
                     NYEQYEFLDEAETIVKYAKELQAKNVKAIVVLAHVPATSKNDIAEGEAAEMMKKVNQL
                     FPENSVDIVFAGHNHQYTNGLVGKTRIVQALSQGKAYADVRGVLDTDTQDFIETPSAK
                     VIAVAPGKKTGSADIQAIVDQANTIVKQVTEAKIGTAEVSVMITRSVDQDNVSPVGSL
                     ITEAQLAIARKSWPDIDFAMTNNGGIRADLLIKPDGTITWGAAQAVQPFGNILQVVEI
                     TGRDLYKALNEQYDQKQNFFLQIAGLRYTYTDNKEGGEETPFKVVKAYKSNGEEINPD
                     AKYKLVINDFLFGGGDGFASFRNAKLLGAINPDTEVFMAYITDLEKAGKKVSVPNNKP
                     KIYVTMKMVNETITQNDGTHSIIKKLYLDRQGNIVAQEIVSDTLNQTKSKSTKINPVT
                     TIHKKQLHQFTAINPMRNYGKPSNSTTVKSKQLPKTNSEYGQSFLMSVFGVGLIGIAL
                     NTKKKHMK"
BASE COUNT         7012 a         3241 c         4270 g         6823 t
ORIGIN      
        1 tattataata gtagtgtaaa taaataagga agtaggtgtt tatgatgaaa aaaggacaag
       61 taaatgatac taagcaatct tactctctac gtaaatataa atttggttta gcatcagtaa
      121 ttttagggtc attcataatg gtcacaagtc ctgtttttgc ggatcaaact acatcggttc
      181 aagttaataa tcagacaggc actagtgtgg atgctaataa ttcttccaat gagacaagtg
      241 cgtcaagtgt gattacttcc aataatgata gtgttcaagc gtctgataaa gttgtaaata
      301 gtcaaaatac ggcaacaaag gacattacta ctcctttagt agagacaaag ccaatggtgg
      361 aaaaaacatt acctgaacaa gggaattatg tttatagcaa agaaaccgag gtgaaaaata
      421 caccttcaaa atcagcccca gtagctttct atgcaaagaa aggtgataaa gttttctatg
      481 accaagtatt taataaagat aatgtgaaat ggatttcata taagtctttt tgtggcgtac
      541 gtcgatacgc agctattgag tcactagatc catcaggagg ttcagagact aaagcaccta
      601 ctcctgtaac aaattcagga agcaataatc aagagaaaat agcaacgcaa ggaaattata
      661 cattttcaca taaagtagaa gtaaaaaatg aagctaaggt agcgagtcca actcaattta
      721 cattggacaa aggagacaga attttttacg accaaatact aactattgaa ggaaatcagt
      781 ggttatctta taaatcattc aatggtgttc gtcgttttgt tttgctaggt aaagcatctt
      841 cagtagaaaa aactgaagat aaagaaaaag tgtctcctca accacaagcc cgtattacta
      901 aaactggtag actgactatt tctaacgaaa caactacagg ttttgatatt ttaattacga
      961 atattaaaga tgataacggt atcgctgctg ttaaggtacc ggtttggact gaacaaggag
     1021 ggcaagatga tattaaatgg tatacagctg taactactgg ggatggcaac tacaaagtag
     1081 ctgtatcatt tgctgaccat aagaatgaga agggtcttta taatattcat ttatactacc
     1141 aagaagctag tgggacactt gtaggtgtaa caggaactaa agtgacagta gctggaacta
     1201 attcttctca agaacctatt gaaaatggtt tagcaaagac tggtgtttat aatattatcg
     1261 gaagtactga agtaaaaaat gaagctaaaa tatcaagtca gacccaattt actttagaaa
     1321 aaggtgacaa aataaattat gatcaagtat tgacagcaga tggttaccag tggatttctt
     1381 acaaatctta tagtggtgtt cgtcgctata ttcctgtgaa aaagctaact acaagtagtg
     1441 aaaaagcgaa agatgaggcg actaaaccga ctagttatcc caacttacct aaaacaggta
     1501 cctatacatt tactaaaact gtagatgtga agagtcaacc taaagtatca agtccagtgg
     1561 aatttaattt tcaaaagggt gaaaaaatac attatgatca agtgttagta gtagatggtc
     1621 atcagtggat ttcatacaag agttattccg gtattcgtcg ctatattgaa atttaaagct
     1681 aaatcttttt agatttagct ttactaatat tatgtgtgtt ggaaaaaaca gtggagattt
     1741 acgtcaaaat tatattattt ggatagtatc gaatagatag aatgtggaga aaagtaggaa
     1801 atagttaaga aaggtattta tgacgcttcg agaattaaca atagaagaat ttaaagaaca
     1861 ttcaggaaat tatgattcac aatcattttt acaaacacct gagatggcta aacttttaga
     1921 aaaacgcggc tatgatgtta ggtatttggg atatcaagta gaaaataaac tagagataat
     1981 cagtttatct tatattatgc cagtcactgg tggttttcaa atgaaaattg attcaggacc
     2041 agttcattca aattctaagt atctaaaaca attttataaa gcattgcaag gctatgccaa
     2101 atccaacggt gttctagaat taatagttga gccttatgat gattaccaat tattcactag
     2161 ttcgggagtt cctagtaatc agggaaatga taatctgatt gaagatttta ccagttcagg
     2221 ttatcaccat gatggtttaa caactggttt tactggtaaa tatttatctt ggcactatgt
     2281 taaaaattta gaaggtgtca cttctgaaac gttactatct tcattctcta agacaggacg
     2341 agctttggtt aagaaagcaa tgtcttttgg aatcaaggtt cgcgttctta aacgtgatga
     2401 gctacattta tttaaagaga taacaacttc tacgtcaaat agacgtgatt atatggataa
     2461 gtccttagat tattatcaag atttttacga tagctttgaa ggcaaggctg aatttgtgat
     2521 tgccacttta aattttagag aatacgacca taacttgcaa ataaaagctg aagcattgga
     2581 aaataagctt aaattgcttg atgaacgttt tagggaaaat gcagactctc ctaagtatca
     2641 taggcaacgc tctgaaataa ttaatcaact ggctagtttt gaaacacgac gacaagaagt
     2701 tcagtcattt attcaaaagt atgataacca agacgttgtt ttagctggta gtctatttgt
     2761 ttatagtctt aaggagacag tttatttctt ttcaggatcc tacacagaat ttaataaatt
     2821 ttatgcacca gcagttcttc aggaatatgt tatgcaagag gctctcaaaa gaggttctac
     2881 tttctataac ctcctaggta tttagggaac ttttgatggt tcagatagta ttcttcgctt
     2941 taaacagaat tttaatggtt atatcattcg taaaatggga acttttaact attatccaag
     3001 tccttttaaa tacaaaggaa ttcagcttct taaaaaggtt ttgaagagaa attaaagtat
     3061 ttgtaaaagc taatctttag attaggcttt tttagtttat tgatagtttt tggaagaaaa
     3121 tgcttgacat tgaaagaaaa agagatataa ttaaattgca aacgatttca attcaaggag
     3181 ggactatgct aaagtcaaag agaaaagaaa ttatcttatc acgacttgag caaaataaga
     3241 gtgttacttt agatgaatta acgtctatct tagagacttc tgaatctaca gttcgcaggg
     3301 atttagatga acttgaatct gaaggttttt taaaaagggt gcatggtggt gctgaattac
     3361 catactcact tggccaggaa ttaagtaatc aagaaaaatc tatcaaaaac gttcaaaaaa
     3421 aattggatat tgctagacaa actgcaaagc tgatagcaaa gcaagatgtt atatttattg
     3481 atgccggaac tacgacagaa ttattaattg attttctccc tcacgagcaa ttaacggtgg
     3541 tgacgaattc tattcatcat gctgcaaagt tagttgatag agggataaaa acaatcatta
     3601 tcggaggagc ggttaaacat tcaacagatg ctagtattgg ccaggtagcc attaatcaga
     3661 ttaggcagat aacggttgac aaagcttttc tggggatgaa tggtatagat gaagtttatt
     3721 taactacacc agatttagaa gaagctgcta ttaaagaagc tattattaat aatagtcaac
     3781 aaacctttat tctaatggat tcatctaaaa ttggacaagt tacatttgct aaagtgaaag
     3841 agataaatga cattaattta gtaactaata agactgattc agaacttatg acaataataa
     3901 aagagaaaat gaaggtgatt caagtatgat ttatacagta acattaaatc catcgattga
     3961 ttttattgtt cgtctagata cactattgtt gggtagtgtt aaccgtatga ctagcgatga
     4021 taaatatgta ggtggtaaag gaattaatgt tagtcgcata ttaaaacgct taaaaattga
     4081 caatactgct acaggtttta tcggtggatt tacaggacat tttgttgaag atggacttgt
     4141 atcagaaggt atcaaaactg actttgtttc tgttaatgaa gatacacgta tcaacgttaa
     4201 agtaaaagca aaaattgaaa ctgaaatcaa tggcgggggt cctaggataa caaatgaaca
     4261 acttcatcgt ctagagaaac ttctttcgag actaacacct gaggacacag tagttttcgc
     4321 aggttcagca ccagctagcc ttggaaataa ggtctataat acattgattc caattgctaa
     4381 aaagacagga gcggaagttg tttgcgattt tgaaggtcag acacttttag acgctcttgc
     4441 ttatcagcct cttttagtaa aaccgaataa ccatgaatta gcagatattt ttggcgtaga
     4501 gctagaaggt ctgcctgata tcgaaaaata tgcacataag attctagata aaggagctaa
     4561 gaatgttatc gtttctatgg caggggatgg tgcattgtta gtaactccag aagcttcata
     4621 ttttgcaaaa ccgattaagg gtgaagtgaa aaattccgtt ggggctggtg attcaatggt
     4681 agctggcttt acaggagaat ttgttaagtc aaaaaatcca gtagaagcac taaaatgggg
     4741 cgtagcttgt ggtacagcaa ccactttctc agatgattta gcaacagctg agtttatcca
     4801 agatatttat aataaagttg aggtagaaaa attatgaaaa tccaagacct actaaaaaaa
     4861 gaagttatga ttatggattt aaaagcaacc agtaaagagg ctgcaattga tgaaatgatt
     4921 acaaaattag ttgacacagg tgttgtgaca aactttgcta tttttaaaga tggtatcatg
     4981 aagcgtgagg cacaaacatc aactggatta ggcgatggta tcgctatgcc acatagtaaa
     5041 aatgctgccg ttaaagaagc tactgtcctt tttgcaaaat ctgcatcagg tgttgattac
     5101 gaagcacttg atggacagcc aactgattta ttctttatga ttgctgcgcc agatggtgct
     5161 aatgatacgc atttagctgc cttggcagaa ctatctaaat atcttttaaa agaagggttc
     5221 gctgatcaat tacgtcaagc aaaaacgcca gatgacatta ttgctacttt tgatagtaat
     5281 agcatttctc aagaaacagt agctccacaa acagtacaat caacaagtaa aggttcagat
     5341 tatattgttg cagtaacagc ttgcactacg ggaattgccc acacgtatat ggcagaagaa
     5401 gctttaaaga aaaaagcagc tgaaatgggt gtaggtatta aagttgaaac aaatggcgct
     5461 tctggtgtag gaaataagtt gacttcaagt gatattgcaa gggctaaggg tgttattatt
     5521 gcagcagata aggctgtaga gatggatcgt tttgatggca aacctttggt atcacgtccg
     5581 gtagctgatg gtattaaaaa aagtgaagac ttaattaaca ttattctcga taataaggca
     5641 caaacctatc atgcaaaaaa tcaaaatgat aaacaatcag gtgaatctga tggaaaatca
     5701 ggcttaggct ctgcttttta taaacacctt atgggtggtg tatcacaaat gttaccattc
     5761 gttattggtg gtggtattat gattgcgatt gctttccttt ttgataacat acttggtgta
     5821 ccaaaagacc aattaagtaa tttaggttca taccatgaaa ttgcagcctt attcaaaaat
     5881 atcggtggcg cagcatttgc atttatgtta ccagtattgg caggttatat cgcttattca
     5941 atagctgaaa aaccaggact agtagctggc ttcgtagctg gttcaattgc atcaagtgga
     6001 ctggcattcg gtaaagtacc ttttgcagaa ggtgggaaag caaccttagc attagcaggt
     6061 gtgccatcag gtttcttagg ggctttagta ggtggtttct tagctggtgg agttatccta
     6121 ctacttagaa aattactttc aggtctccca aaatcgcttg aaggtattaa atcaattctt
     6181 ctatacccat tgttaggtgt tttgattaca ggtttcttga tgttgctcgt aaacattcca
     6241 atggcagcta tcaacacagc tcttaacact ttcttacaag gtttatctgg tagttcagca
     6301 gtcttgatgg gcttactagt tggtggtatg atggctgtag atatgggtgg gcctgttaat
     6361 aaagcagctt atgtttttgg tacaggtact cttgcagcaa ctgttgcaaa tggcggctct
     6421 gttgttatgg cagctgtaat ggctggagga atggttccac cgttagcagt atttgtggca
     6481 actttattat ttaaagataa atttaataat gaagaacgtc aatctggtct aacaaatatc
     6541 gttatggggt tatcttttat cactgaaggt gctattccgt ttggtgctgc tgacccagct
     6601 cgtgcaatcc ctagctttat tgtaggttca gccttaactg gagcacttgt tggtttagct
     6661 ggtatcaagt tgatggctcc tcatggaggg atttttgtca tcgcattaac aagtaatcct
     6721 ttactttata ttctatttat tctaattgga gcggtagtct caggtgtact ctttggcttg
     6781 ttccgtaaaa caaaatagtt aatctcaact tatccaaatt tacattgaat aaaaggtgaa
     6841 taaactatcg ttattgggtc tattcgcctt tttatgttgt aaaagaatgg tagattaatc
     6901 attaataatt gctaatcttt tttcaatagg atatgattta tttaagcaaa aaaatggata
     6961 atatgattga ggaaaggagg taagcaatga ttaaatggaa tgaaaaagaa acaaaactag
     7021 ttgatacaag tttttcaatt atgttgtatc taaaaacgct atttgcagtc ggacaagtag
     7081 tagctggcgt tttattatat tattttaaac ctacacaaat aaaacatctc ctatttcttt
     7141 taactgaagg tgaagttcac ggtgggattc atggttttat ttctgctcat ttcttgaaaa
     7201 taagcagtta tattaatgat tcggctagtc atttagccgc tatctatcta ttaattcatg
     7261 gcggtatcaa attagtagca cttattttat tacataaaga gaaattattt gcttaccctt
     7321 tatcaatttt agttttcgtt gggtttatca ttttccaact aagggaatat ttttctagtc
     7381 atagcatttt aatgattttt ctatgtgtat ttgatgttat catgataatt ttaacatggt
     7441 tagaatatca gaaaatgaaa ttgaattttt caaaaaggtg acttccccca tgccttagcg
     7501 cttgttttta taagcgcttt tttgctacaa tatgactatg aaaactattg aagaaattta
     7561 caaaaattat cctaatatcc cctatatctc acctgaaaga gacttggctg aaattaattt
     7621 tagtaaagtt gttcctagga aaaatatgga agaaactagt gaaggactct tgcctggaga
     7681 tattatctta ttatggcgta tacagtttgg aactttcaca actgaaacaa gtttttcaaa
     7741 atattttgaa tacatttatg gtataaatgg aaaagaacac ctagaatttt taataaaaaa
     7801 tggatttgta agaatggagt caccgcttga ttctttagat cacttatctg ctccactttt
     7861 aaaactattc ttaaaagaaa aaaacgtaaa aggcttgtca aagatgaaac gctctgattt
     7921 agatcaggca atagctattg cttttacaga agaggaatta gggaagttgt ttgttgtcag
     7981 agggttggca cttacagaaa aaggtttagc agccttgagt aacaatcaag aagtgattga
     8041 tagacatcct aagaagaagt tctagattcc cacctgcttt aagaatctac aaatttatgc
     8101 tataataata catattgtct atcaaggaga aaatacatgg aagtcattcg tgaacaagaa
     8161 tttgtcaatc aataccacta tgatgcgcgc aatttagaat gggaagagga aaatggtacc
     8221 ccaaaaacaa attttgaagt tactttccaa ttagctaata gagatgaagc agcaaaagta
     8281 acgagcattg tagcagtgtt acaatttgtt attgtacgtg atgagtttgt tatttctggt
     8341 gtcatttcac aaatggctca tatccaaggg cgccttatca atgagcctag tgagtttagt
     8401 caagatgaag ttgagaatct agcagctcct cttttagaaa tcgttaaacg tctgacttat
     8461 gaagttacag aaattgcttt agatagacca ggagtaacat tggagtttaa tagttaatga
     8521 aattagcact tattacggat acctcagctt acttgcctga ggctatcgaa aatcatgaag
     8581 atgtctatgt tttagatatt ccaattatca ttgatggtaa aacttatatt gagggacaaa
     8641 atctgacatt agaccaatat tatgataagc tagcggcatc aaaagaatta cctaaaacaa
     8701 gtcagcctag cttagctgaa ctagatgatc tcttatgtca attggagaaa gaagggtata
     8761 cacacgtcct tggtttattc attgcagcgg ggatatcagg tttctggcag aatattcagt
     8821 ttcttattga agaacatcca aatttgacaa ttgcttttcc ggatactaaa ataacttcag
     8881 caccacaggg caatttagtt agaaatgctt tgatgtgttc acgtgaaggg atggattttg
     8941 atgtcatagt taacaagata caatcacaaa ttgaaaaaat tgaaggattt attgttgtta
     9001 atgatttgaa tcatctagta aaaggtggta gattgtcaaa cggttcagct atcattggta
     9061 accttttaag tatcaagccg gtcttacatt ttaatgaaga aggtaaaatc gttgtctatg
     9121 agaaagtgcg tacagagaaa aaagccttaa aacgcttggc tgagattgtc aaagaaatga
     9181 cagcagatgg tgaatatgat attgctatta ttcactctag agcacaagat aaggcagaac
     9241 aattatataa tttgttagct aaagctggtc ttaaagacga tttagaaatt gtatcatttg
     9301 gtggcgttat tgcaactcat cttggtgaag gtgcggttgc ctttggtatt acccccaaaa
     9361 attgatgtgt gataaaaaga gttgcttgga ggttgatcct tattgcagat atcaaagaag
     9421 aagttaggag attctttttt gataatttga gtaggtaggg agtgtccctt aggcagttct
     9481 ttttgtttct gaatccattt ataagaaata ataatgcgac tttagttcgt ttagatttgg
     9541 aatagtatga tgagattaaa ttatttgcct tctgagtttc agaaggcttt accaatatta
     9601 aaaaagataa aaaaagccgg ttatgaagca tactttgtag gtggtagtgt tcgggatgtt
     9661 ttacttgacc gtccaattca tgatgttgat atagcaacgt catcttatcc agaagaaaca
     9721 aagcagattt ttaaaaggac agttgatgtt ggtattgaac atggaacagt cttagtattg
     9781 gaaaaaggtg gtgaatatga gattaccact tttaggactg aggaggttta tgttgattat
     9841 aggcgtccga gtcaggtcaa ttttgttagg tcgctagaag aagatctaaa acgtcgagat
     9901 ttcacagtta atgcctttgc tttaaatgaa gatggagaag ttattgatct ctttcatgga
     9961 ttggatgatt tagataatca ccttcttaga gcagtaggac tcgccagtga gcgttttaat
    10021 gaagatgctc ttcgaattat gcgaggtttg cgtttttctg ctagtctaaa ttttgatatc
    10081 gaaacaacaa cttttgaggc aatgaaaaag cacgcgtcat tattggagaa aatatctgtt
    10141 gagcgttctt ttattgaatt tgataaactt ctattagcac cttattggcg taaaggaatg
    10201 ctggcactaa tagatagtca tgcttttaat tatctaccat gcttaaaaaa tagggaatta
    10261 caattaagcg cctttttgtc ccagttagat aaagattttt tatttgagac atcagaacaa
    10321 gcttgggcat cactcatctt gagtatggaa gttgaacaca caaagacttt tttaaaaaaa
    10381 tggaagacat caactcactt tcaaaaagat gttgagcata tagtggatgt ttatcgtatt
    10441 cgtgaacaaa tgggattgac taaagaacat ctttatcgtt atggaaaaac tataataaaa
    10501 caagcggaag gtattcgcaa agcaagaggc ttgatggttg atttcgaaaa aatagaacaa
    10561 ctagatagtg agttagcaat ccatgatagg catgagatag ttgtcaatgg tggcacctta
    10621 atcaagaaat taggaataaa acctggtcca cagatgggag atattatctc tcaaattgaa
    10681 ttagccattg ttttaggaca actgattaat gaagaagagg ctattttaca ttttgttaag
    10741 cagtacttga tggattagag aggattatat gagcgatttt ttagtagatg gattgactaa
    10801 gtcggttggt gataagacgg tctttagtaa tgtttcattt atcatccata gtttagaccg
    10861 tattgggatt attggtgtca atggaactgg aaagacaaca ctattagatg ttatttcggg
    10921 tgaattaggt tttgatggtg atcgttcccc tttttcatca gctaatgatt ataagattgc
    10981 ttatttaaaa caagaaccag actttgatga ttctcagaca attttggaca ccgtactttc
    11041 ttctgactta agagagatgg ctttaattaa agaatatgaa ttattgctta atcactacga
    11101 agaaagtaag caatcacgtc tagagaaagt aatggcagaa atggattctt tagatgcttg
    11161 gtctattgag agcgaagtca aaacagtatt atccaaatta ggtattactg atttgcagtt
    11221 gtcggttggt gaattatcag gaggattacg aagacgtgtt caattagcgc aagtattatt
    11281 aaatgatgca gatttattgc tcttagacga acctactaac cacttagata ttgacactat
    11341 tgcatggtta acgaattttt tgaaaaatag taaaaagaca gtgcttttta taactcatga
    11401 tcgttatttt ctagacaatg ttgcaacacg tatttttgaa ttagataagg cacagattac
    11461 agaatatcaa ggcaattatc aggattatgt ccgacttcgt gcagaacaag acgagcgtga
    11521 tgctgctagt ttacataaaa agaaacagct ttataaacag gaactagctt ggatgcgtac
    11581 tcagccacaa gctcgtgcaa cgaaacaaca ggctcgtatt aatcgttttc aaaatctaaa
    11641 aaacgattta caccaaacaa gcgatacaag cgatttggaa atgacatttg aaacaagtcg
    11701 aattgggaaa aaggttatta attttgaaaa tgtctctttt tcttacccag ataaatctat
    11761 cttgaaagac tttaatttgt taattcaaaa taaagaccgt attggcatcg ttggagataa
    11821 tggtgttgga aagtcaacct tacttaattt aattgttcaa gatttacagc cggattcggg
    11881 taatgtctct attggtgaaa cgatacgtgt aggttacttt tcacaacaac ttcataatat
    11941 ggatggctca aaacgtgtta ttaattattt gcaagaggtt gcagatgagg ttaaaactag
    12001 tgtcggtaca acaagtgtga cagaactatt ggaacaattt ctctttccac gttcgacaca
    12061 tggaacacaa attgcaaaat tatcaggtgg tgagaaaaaa agactttacc ttttaaaaat
    12121 cctgattgaa aagcctaatg tgttactact tgatgagccg acaaatgact tagatattgc
    12181 tacattaact gttcttgaaa attttttaca aggctttggt ggtcctgtga ttacagttag
    12241 tcacgatcgt tactttttag ataaagtggc taataaaatt attgcgtttg aagataacga
    12301 tatccgtgaa ttttttggta attatactga ttatttagat gaaaaagcat ttaatgagca
    12361 aaataatgaa gttatcagta aaaaagagag taccaagaca agtcgtgaaa agcaaagtcg
    12421 taaaagaatg tcttactttg aaaaacaaga atgggcgaca attgaagacg atattatgat
    12481 attggaaaat actatcactc gtatagaaaa tgatatgcaa acatgtggta gtgattttac
    12541 aaggttatct gatttacaaa aggaattaga tgcaaaaaat gaagcacttc tagaaaagta
    12601 tgaccgttat gagtacctta gtgagttaga cacatgatta tccgtccgat tattaaaaat
    12661 gatgaccaag cagttgcaca attaattcga caaagtttac gcgcctatga tttagataaa
    12721 cctgatacag catattcaga ccctcactta gatcatttga cctcatacta cgaaaaaata
    12781 gagaagtcag gattctttgt cattgaggag agagatgaga ttattggctg tggcggcttt
    12841 ggtccgctga aaaatctaat tgcagagata cagaaggtgt acattgcaga acgtttccgt
    12901 ggtaaggggc ttgctactga tttagtgaaa atgattgaag tagaagctcg aaaaattggg
    12961 tatagacaac tttatttaga gacagccagt attttgagta gggcaactgc ggtttataag
    13021 catatgggat attgtgcctt atcgcaacca atagcaaatg atcaaggtca tacagctatg
    13081 gatatttgga tgattaaaga tttataagtt gaaagtggat tagtgaacat ggattaatta
    13141 ttttgagata agaggaaaga aaaggagaca tatatggcat atatttggtc ttatttgaaa
    13201 aggtacccaa attggttatg gcttgattta ctaggagcta tgctttttgt gacggttatc
    13261 ctaggaatgc ccacagcctt agcgggtatg attgataatg gcgttacaaa aggtgatcgg
    13321 actggagttt atctgtggac gttcatcatg tttatatttg ttgtactagg tattattggg
    13381 cgtattacga tgacttacgc atctagtcgc ttaacgacaa caatgattag agatatgcgt
    13441 aatgatatgt atgctaagct tcaagaatac tcccatcatg aatatgaaca gataggtgta
    13501 tcttcactag tgacacgtat gacaagcgat acttttgttt tgatgcaatt tgctgaaatg
    13561 tctttacgtt taggcctagt aactcctatg gtaatgattt ttagcgtggt tatgatacta
    13621 attacgagtc catctttagc ttggcttgta gcggttgcga tgcctctttt ggtaggagtt
    13681 attttatatg tagctataaa aacaaaacct ttatctgaaa gacaacagac tatgcttgat
    13741 aaaatcaatc aatatgttcg tgaaaattta acagggttac gcgttgttag ggcctttgca
    13801 agagagaatt ttcaatcaca aaaatttcaa gtcgctaacc aacgttacac agatacttca
    13861 actggtcttt ttaaattaac agggctaaca gaaccacttt tcgttcaaat tattattgca
    13921 atgattgtgg ctatcgtttg gtttgctttg gatcccttac aaagaggtgc tattaaaata
    13981 ggggatttag ttgcttttat cgaatatagc ttccatgctc tcttttcatt tttgctattt
    14041 gccaatcttt ttactatgta tcctcgtatg gtggtatcta gccatcgtat tagagaggta
    14101 atggatatgc cgatttctat taatcctaat accgaaggtg ttacggatac gaaacttaaa
    14161 gggcatttag aatttgataa tgtaacattc gcttatccag gagaaacaga gagtcccgtt
    14221 ttgcatgata tttcttttaa agctaagcct ggagaaacaa ttgcttttat tggttcaaca
    14281 ggttcaggaa aatcttctct tgttaatttg attccacgtt tttatgatgt gacacttgga
    14341 aaaatcttag tagatggagt tgatgtaaga gattataacc ttaaatcact tcgccaaaag
    14401 attggattta tcccccaaaa agctctttta tttacaggga caataggaga gaatttaaaa
    14461 tatggaaaag ctgatgctac tattgatgat cttagacaag cggttgatat ttctcaagct
    14521 aaagagttta ttgagagtca ccaagaagcc tttgaaacgc atttagctga aggtgggagc
    14581 aatctttctg ggggtcaaaa acaacggtta tccattgcta gggctgttgt taaagatcca
    14641 gatttatata tttttgatga ttcattttct gctctcgatt ataagacaga cgctacttta
    14701 agagcgcgtc taaaagaagt aaccggtgat tctacggttt tgatagttgc tcaaagggtg
    14761 ggtacgatta tggatgctga tcagattatt gtccttgatg aaggcgaaat tgtcggtcgt
    14821 ggtacccacg ctcaattaat agaaaataat gctatttatc gtgaaatcgc tgagtcacaa
    14881 ctgaagaacc aaaacttatc agaaggagag tgattgtatg agaaaaaaat ctattttttt
    14941 gagattatgg tcttacctaa ctcgctacaa agctactctt ttcttagcga tttttttgaa
    15001 agttttatct agttttatga gtattctgga gccttttatt ttagggttag cgataacaga
    15061 gttgactgct aaccttgttg atatggctaa gggagtttct ggggcagaat tgaacgttcc
    15121 ttatattgct ggtattctga ttatttattt tttcagaggt gttttctatg aattaggttc
    15181 ttatggctca aattatttta tgactactgt cgttcaaaaa agtatccgtg atatccgtca
    15241 tgatttgaat cgtaaaatca ataaagttcc tgtatcgtat tttgataagc accaatttgg
    15301 agatatgttg ggacgtttta caagtgatgt tgaaacggtc tcaaatgctt tacaacagag
    15361 ctttttacag attatcaatg cttttttaag tattattcta gtagtcgtta tggtacttta
    15421 tctaaatgta ccattagcca tgattatcat tgcttgtatt cctgtaactt attttagtgc
    15481 acaagcgata ctaaaaagat cccaacctta ctttaaagaa caggcaaaga ttctgggaga
    15541 actgaatggt tttgtacaag agaaattaac tggtttcaat atcataaaat tatatggtag
    15601 agaagaggcg tctagtcaag aatttcgtga tataacggat aatctacgtc atgtaggttt
    15661 caaagctagc ttcatttcag gaattatgat gccagtattg aatagtatct ctgattttat
    15721 ttatcttatc attgcatttg ttggcggttt acaagttatt gctggtacct taacaattgg
    15781 taacatgcag gcatttgtgc agtatgtttg gcaaattagt caacccgttc aaaccattac
    15841 acaattagca ggtgttttgc aaagtgcaaa atcttcctta gaacgtattt ttgaagtctt
    15901 agatgaagaa gaggaagcca atcaagtcac tgaaaaattg agccatgact taaccggtca
    15961 agtcagcttt catggtgtcg attttcacta ttctccggat aagccactta tccgcgactt
    16021 taacttagat gtagaaccag ggcaaatgat tgcaattgta gggccaacag gtgcaggtaa
    16081 gacaacactt ataaacttac tcatgagatt ctacgatgtt agtgaaggtg ctatcaccgt
    16141 agatggtcac gatattagac acctatcacg ccaagatttc cggcaacaat ttggcatggt
    16201 gttgcaagat gcttggttat atgaaggtac aataaaagaa aatcttagat tcggtaatct
    16261 tgaagccagt gatgaggata tagttgctgc tgccaaagct gcgaatgttg accactttat
    16321 tcgaacgtta cctggtggct acaatatggt gatgaatcaa gaatcaagca atatctcctt
    16381 aggacaaaag caactgttga ctattgcacg tgctttatta gcagatccta aaattttaat
    16441 tttagatgaa gcgacttcat ctgttgatac aaggttggaa ttgcttattc aaaaagctat
    16501 gaaaaaattg atggaaggaa gaactagttt tgtcattgca caccgtctat caacgattca
    16561 agaggcagac aacattttag ttctaaagga tggtcaaatc atcgaacaag gtaatcacca
    16621 gaaactccta gcagataaag gattttatta cgaattatat aatagccaat tttcaaatag
    16681 taaatgacgt catcaataga taaaaaaagg acacgaaaca tgtcctttta ataattaata
    16741 cgagtaaaag tgtaaatagc cgaagctcca attgctatta atataaaggc tacaaatctt
    16801 ccaatgatgg ctgctgataa ggtaggttga accagtaaaa taatacctaa aatgagtgca
    16861 atataagccg aacgtttata ggatttggca cctgggaaat taattttttg catttggaaa
    16921 ccacccaaaa gtcgtaaaat accagatacc agtagccagt atgctaaaat agtcatgaca
    16981 gtatttgaaa gtgacattgc tgagctggat agtaaaatga taccaaaaat aatagcaagg
    17041 atactctgaa ccagatacca agggctacgg tgataagaat cagcttgcaa gtagcgtact
    17101 aaatttgaaa tgccataaat aaagataaat aaagcaatga accaacccag agttgcaaca
    17161 accgttacag gatttataaa gagatagaag ccagctaata aggcaattat acctccaaca
    17221 agtgggaacc atttcatata gatactccaa tcaaatattt gtctcattat agaacgaaag
    17281 tcagtaaaag tcaaataatt acctttaaaa ttgctcctaa taatgatacc atagcaaata
    17341 gttgacaaga aaattctgac aatttataat gctaactata ttaaaacaat acggaggaaa
    17401 ataaatgaca agcggaaaga catatgtagc aagtgtttta gagaaagtga agaagcaaaa
    17461 tgagcatgag gaagaatttc tacaagcggt tgaagaggtt tttgagtcac tagtaccagt
    17521 ttttgataaa taccctcagt acattgaaga aaatctttta gaacgtttgg ttgagcctga
    17581 gcgcgtgatt tcttttagag ttccatgggt agatgataaa ggccaggttc aagttaaccg
    17641 aggatatcgt gtacaatttt catcagctat tggtccttat aaaggtggtt tacgttttca
    17701 ccctacagta acacaatcta ttgtcaaatt tttaggattt gaacaaattt ttaagaattc
    17761 actgacaggt ctaccaattg gtggtggcaa aggagggtca aactttgatc caaaagggaa
    17821 atcagataat gaagtgatgc gtttcacaca aagttttatg actgaacttc aaaaacacat
    17881 tggaccagat ttagatgtgc cagctggcga tattggagta ggtggacgtg aaattggtta
    17941 tctttatggt caatataagc gtttaaatgg ttatcaaaat ggggttttaa ccggaaaagg
    18001 actgacttat ggtggctctt tggctcgtac agaagcaact ggttatgggg cagtctattt
    18061 tgctaaagaa atgctagcag cgcgtggtca agatttaact ggcaaagttg cactggtttc
    18121 gggatctggt aacgtggcta tttatgctac tgaaaaatta caagagttgg gtgcaactgt
    18181 tgtagctgta tcagattctt caggttatgt ttatgatcca gacggtattg accttgaaac
    18241 attgaaacag ataaaagagg ttgaaagagc acgtattgtt aaatatacgg agaaacatcc
    18301 aaaagctaat ttcacaccag cagatcaagg tagtatctgg tctattaaag ctgatttagc
    18361 tttcccgtgt gctactcaaa acgaattaga cgaagaagac gctaagttat tagttgaaaa
    18421 tggtgtcttg gcagtaacag aaggagcaaa tatgccatct accttgggtg ctattaaagt
    18481 tttccaaaag gcaggggttg cttttggtcc tgctaaagct gccaatgcag ggggagtagc
    18541 tgtttcagcg cttgaaatgg cacaaaattc tagtcgtagg gcatggactt ttgaagaagt
    18601 tgatcaagaa ttgcaacgta ttatgaaaac tatttttgtg aatgcttccg aagcggctga
    18661 tgaatttggc gatagtggta acctggttct aggtgctaac attgcaggct ttttaaaagt
    18721 tgctcaagct atgtctgcac aaggtattgt ttaatctctt atcatagtcc ttagctaacc
    18781 tatggtatac tagtattatc ataatagagt taggttaaaa taaatgataa aaccaattgt
    18841 tagagatact ttctttttac aacaaaaatc acaaatggct agtagagcag atgtgagctt
    18901 agcaaaagat ttacaggaga ctttgcatgc taatcaaaat tattgtgtag gtatggcagc
    18961 aaatatgatt ggtagtctga agcgtgtcat tatcatcaat gtgggtatta caaatttagt
    19021 aatgtttaac cctgtagtag ttgcaaaatc ggatccatat gagacagaag aatcttgcct
    19081 ttctctcgta ggttgcagaa gtacacaacg atattgtcac attacgattt cttatcgtga
    19141 tatcaattgg aaggaacagc aaatcaagtt gacagatttt ccagctcaaa tttgtcaaca
    19201 cgagttagat catttggaag gcatattaat ataaaaatag gtttggatat tttatccaaa
    19261 cctattttta ttatttcata tgttttttct ttgtatttaa agcaattcct ataagtccaa
    19321 caccaaagac agacataagg aatgattgtc catattcaga gtttgttttt ggtaattgtt
    19381 ttgattttac agtagtggag tttgatggtt tgccataatt tctcataggg ttaatagctg
    19441 taaattggtg taattgtttt ttgtgaattg tagttacagg gttgattttt gtagattttg
    19501 attttgtttg gtttaaagtg tctgatacaa tctcttgtgc tacaatattt ccttgtcgat
    19561 ctaaataaag tttcttaata atgctatgtg taccatcatt ttgtgtaata gtttcattaa
    19621 ccatcttcat agtgacatag attttaggtt tattatttgg aacgctcact tttttaccag
    19681 ctttttctaa atcagtgata taggccataa atacctctgt atcggggtta atggctccta
    19741 gaagtttggc atttctgaag cttgcaaagc catcaccacc accgaataaa aagtcattga
    19801 taactaattt gtattttgca tcaggattga tttcctcacc atttgattta taagctttta
    19861 caactttaaa tggtgtttct tccccgccct ctttattatc tgtgtaagtg tatcgcagac
    19921 cagctatttg aaggaagaaa ttttgttttt ggtcgtattg ttcgttgagt gctttataaa
    19981 gatctctacc agtaatttcg acgacttgta agatattacc aaaaggttga actgcttgtg
    20041 cagctcccca ggtgattgtt ccatctggtt tgatgagtaa gtcagcacga atgccaccat
    20101 tatttgtcat ggcaaaatcg atatctggcc agctttttcg agcaattgct agttgagcct
    20161 ctgtgatgag gctgcctacc ggactaacat tatcttgatc aacagaacgc gtaatcatga
    20221 cacttacctc ggcagtacca attttagctt ctgttacttg tttaacgata gtattagctt
    20281 ggtcaacaat ggcttgaata tcggcactac ctgttttttt accaggagca actgcaatta
    20341 ctttagctga aggggtctca atgaaatctt gtgtatcagt atctaagaca ccacgtacat
    20401 cagcataggc ttttccttga gagagcgctt gtacaatacg agttttacca acaagaccat
    20461 ttgtatattg atgattgtgt ccagcaaaga caatatctac gctattttca gggaagagtt
    20521 gattgacttt tttcatcatt tctgctgctt caccttcagc aatatcattt ttacttgttg
    20581 caggtacatg tgcgagaact acaatagctt tgacattttt agcttgtaat tctttggcgt
    20641 atttaacgat tgtttcagct tcatctaaaa attcatattg ttcataattt ttacgtaaga
    20701 caaggtttgg gatgtctttg gtgacaatcc cgataaagcc aacgttcaca cttttgttat
    20761 ttacaggaat atttttaata gcgtaaggct tccaattgta aggaatttgt ttgttaactt
    20821 tatcaataac atttgccact acaatttctt gttttgcagc ttcatgtggg tatgatttcg
    20881 taatattatt aatattagaa tctggagcag gggctttacc agtaacgata cgattatatt
    20941 ctgccaaccc ttcatcaaat tcatggttac ccaatgtgcc atactcaaca ttcattgcat
    21001 taaaattttt gacagttggt tcatcttgaa gaagcccaga gttggctgga cttgctccaa
    21061 ccatatcgcc tgcttgaacc ctaatgcttt caccattagg gttagtttgt ttgaaatctt
    21121 tttgagcgtc atccatataa gcatctaatt gagcagcagt accagcatta gcaacttttc
    21181 catcaggcat atttgctgtt ccagtattgt caagtgcacc atgaaagtca ttgacgccta
    21241 taacttggac accgacttgg tccgcgaaca cgcttgagaa cataatagaa gtcccagcga
    21301 ctaaaccaag aacactactt ttcaaaataa ttttcttttt cattta
//
LOCUS       DBJYSH010000020        21154 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.020, whole genome shotgun sequence.
ACCESSION   DBJYSH010000020 DBJYSH010000000
VERSION     DBJYSH010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 21154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21154
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.020"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            complement(<1..638)
                     /locus_tag="ACUBV3_000624"
     CDS             complement(<1..638)
                     /locus_tag="ACUBV3_000624"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019770913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - metabolic compound salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HHU3847151.1"
                     /translation="MSQFDRIHLVVLDSVGIGAAPDANDFVNAGVPDGASDTLGHISK
                     TVGLAVPNMAKIGLGNIPRPQALKTVPAEENPSGYATKLQEVSLGKDTMTGHWEIMGL
                     NITEPFDTFWNGFPEDIITKIEDFSGRKVIREANKPYSGTAVIDDFGPRQMETGELII
                     YTSADPVLQIAAHEDIIPLEELYRICEYARSITMERPALLGRIIARPYVGEPG"
     gene            complement(705..1376)
                     /gene="deoC"
                     /locus_tag="ACUBV3_000625"
     CDS             complement(705..1376)
                     /gene="deoC"
                     /locus_tag="ACUBV3_000625"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HHU3847152.1"
                     /translation="MEVKDILKTVDHTLLATTATWPEIQTILDDAMAYETASACIPAS
                     YVKKAAEYVSGKLAICTVIGFPNGYSTTAAKVFECQDAIKNGADEIDMVINLTDVKNG
                     DFDTVEEEIRQIKAACQDHILKVIVETCQLTKEELIELCGVVTRSGADFIKTSTGFST
                     AGATFEDVEVMAKYVGEGVKIKAAGGISSLEDAEKFIALGASRLGTSRIIKIVKNQKV
                     EEGTY"
     gene            complement(1406..2608)
                     /locus_tag="ACUBV3_000626"
     CDS             complement(1406..2608)
                     /locus_tag="ACUBV3_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HHU3847153.1"
                     /translation="MQFIYSIIGILLVLGIVYAISFNRKSVSLSLIGKALIVQFIIAL
                     ILVRIPLGQQVVSVVSTGVTKVINCGQAGLNFVFGSLADSGAKTGFIFAIQTLGNIVF
                     LSALVSLLYYVGILGFVVKWIGKGVGKIMKSSEVESFVAVANMFLGQTDSPILVSKYL
                     GRMTDSEIMVVLVSGMGSMSVSILGGYIALGIPMEYLLIASTMVPIGSILIAKILLPQ
                     TEPVQKIDDIKMDNKGNNANVIDAIAEGASTGAQMAFSIGASLIAFVGLVSLINMMLS
                     GLGIRLEQIFSYVFAPFGFLMGFDHKNILLEGNLLGSKLILNEFVSFQQLGDLIKSLD
                     YRTALVATISLCGFANLSSLGICVSGIAVLCPEKRGTLARLVFRAMIGGIAVSMLSAF
                     IVGIVTLF"
     gene            complement(2629..3408)
                     /gene="udp"
                     /locus_tag="ACUBV3_000627"
     CDS             complement(2629..3408)
                     /gene="udp"
                     /locus_tag="ACUBV3_000627"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HHU3847154.1"
                     /translation="MQNYSGEVGLQYHLQIRPGDVGRYVIMPGDPKRCAKIAEHFDNA
                     VLVADSREYVTYTGTLNGEKVSVTSTGIGGPSASIAMEELKLCGADTFIRVGTCGGID
                     LDVKGGDIVIATGAIRMEGTSKEYAPIEFPAVADLEVTNALVNAAKKLGYTSHAGVVQ
                     CKDAFYGQHEPERMPVSYELLNKWEAWKRLGTKASEMESAALFVAASHLGVRCGSDFL
                     VVGNQERNALGMDNPMAHDTEAAIQVAVEALRTLIENDKSQ"
     gene            3566..4303
                     /locus_tag="ACUBV3_000628"
     CDS             3566..4303
                     /locus_tag="ACUBV3_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HHU3847155.1"
                     /translation="MPKNELNNKLNKLKHVQVYNKIFNMIQDGTYSPGMQLPSEPELA
                     GQLNVSRATLRKSLALLQEDHLVKNIRGKGNFIRENSSNLSENGYENRQHPIKTCLTS
                     KITEVELEFRVEVPAEAITASLKQETPVVVIADRWYHTDDGPLAYTLSFIPIELISDA
                     EISLHDTKQLLNFIEEGIYQEGISSHSQSHLGYATSGNFSATKYTLSDHGQFILIQET
                     IFKQEKILMCNKHYVPIEHFELSITSH"
     gene            4316..4612
                     /locus_tag="ACUBV3_000629"
     CDS             4316..4612
                     /locus_tag="ACUBV3_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017766806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3847156.1"
                     /translation="MQNRLLKVAFFITFTELIMGLIVGAYFKLSYFLAPPKEPSMGAG
                     YGMLFSLLFILISYLILLAIIQFLAYQSQSKIIAIIVLLLFSSHYWQILDGLSL"
     gene            complement(4712..6334)
                     /gene="groL"
                     /locus_tag="ACUBV3_000630"
     CDS             complement(4712..6334)
                     /gene="groL"
                     /locus_tag="ACUBV3_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HHU3847157.1"
                     /translation="MAKDIKFSADARSAMVRGVDILADTVKVTLGPKGRNVVLEKAFG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPIGIRRGIETAVSAAVEELKEIAQPVSGKEAIAQVAAVSSRSEKVGEYI
                     SEAMERVGNDGVITIEESRGMETELEVVEGMQFDRGYLSQYMVTDNEKMVSELENPYI
                     LITDKKISNIQEILPLLEEVLKTNRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVVTEDLGLDLKDATMQVLGQSAKVTVDKDSTVIVEGA
                     GDSSAIANRVAIIKSQMEATTSDFDREKLQERLAKLAGGVAVIKVGAATETELKEMKL
                     RIEDALNATRAAVEEGIVSGGGTALVNVIEKVAALKLNGDEETGRNIVLRALEEPVRQ
                     IAYNAGYEGSVIIERLKQSEIGTGFNAANGEWVDMVTTGIIDPVKVTRSALQNAASVA
                     SLILTTEAVVANKPEPEAPTAPAMDPSMMGGF"
     gene            complement(6430..6714)
                     /gene="groES"
                     /locus_tag="ACUBV3_000631"
     CDS             complement(6430..6714)
                     /gene="groES"
                     /locus_tag="ACUBV3_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HHU3847158.1"
                     /translation="MLKPLGDRVIISFVETEEKSVGGFVLAGASHDATKTAKVLAVGD
                     GIRTLTGELVAPSVAEGDTVLVENGAGLEVKDGNEKVTVVRESDIVAVVK"
     gene            complement(6889..7692)
                     /locus_tag="ACUBV3_000632"
     CDS             complement(6889..7692)
                     /locus_tag="ACUBV3_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000054433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HHU3847159.1"
                     /translation="MSLLELVNLHKTFEKGTVNENHVLRGLDLTIEDGDFISVIGGNG
                     AGKSTLLNCIAGLIPIDQGAITLDNQSITKDSVEKRSKDISRVFQDPRMGTATNLTIE
                     ENMAIAHKRGNKRHIFRQSVTDDDRQLFKKSLSQLGLGLENRMKTDAAFLSGGQRQAL
                     TLAMATLVRPKLLLLDEHTAALDPKTSDMVMELTQKVIEEQRLTALMITHNMEHAIAY
                     GNRLVMLYHGKIVVDVKGEAKRNLTVAELMELFHKNSGQQLIDDALVLG"
     gene            complement(7697..8593)
                     /locus_tag="ACUBV3_000633"
     CDS             complement(7697..8593)
                     /locus_tag="ACUBV3_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HHU3847160.1"
                     /translation="MLDIILSGISQGLLWSIMAIGVFITFRILDIADLSAEGAFPMGA
                     AVCALCIVNDINPIVATIAGMLGGMLAGLVSGFLHTKMKIPALLTGIITLTGLYSINL
                     LVLGRSNVSFALKNTLVTMVTRLGLNKLSAVLLIGIVCVGLVILILYLFLNTQLGLAL
                     RATGDNEAMGQANSIKVDRMKMLGYMIGNGLIALSGALLAQNNGYADLNMGVGTIVIG
                     LASIILAEVMIKYLPLGKRLWSIVLGSVLYRMIIVFILTTDIDAQMIKLVSAILLALI
                     LYVPELRAKLGVKTSKSLTKEG"
     gene            complement(8609..9571)
                     /locus_tag="ACUBV3_000634"
     CDS             complement(8609..9571)
                     /locus_tag="ACUBV3_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HHU3847161.1"
                     /translation="MKKITKMVISSVLATVLMTACSQSSQNGQSDVKHIGILQYVEHP
                     SLTATRKGFIKELAKEGYKDGKNIKIEYKNAQGDQSNIQSISEKLIKDNKLVLGIATP
                     AAQSLTTVSTETPILFTAVTDPVSAELVKSMKKPEGLATGTSDMSPIKKQVSLLRKVM
                     PKVKRVGIMYTTSERNSEVQVKQAKKIFQEAGIKTSVKGISSTNDVQDTAKSLMSKTE
                     VIFVPTDNIIASSVTLLGNLSKELKVPVVGGSADMVPSGLLFSYGADYEALGRQTARQ
                     AVKILKGKDVAKVPSEYPQNLKVVVNEDMAKELGIDVSSIKNNK"
     gene            complement(10036..10833)
                     /locus_tag="ACUBV3_000635"
     CDS             complement(10036..10833)
                     /locus_tag="ACUBV3_000635"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HHU3847162.1"
                     /translation="MVRLIFSDIDGTLINSNFKVTPKTRQGIKQIVAQGATFVPISAR
                     MPEAITPIMEQIGIDSYIISYNGALIQDMQQKTIASHTMDGQVALQVCSYVSKHYSKI
                     AWNVYRYHEWYSCDKENEWVQKEEEIVGLQSKEMSLMELEKQDRIHKLLLMGEPSLMG
                     ELENTLKAQYPHLSIAQSAPYFIEIMAPGIEKGKSAKTLADYLDISLADSIAFGDNYN
                     DLNLLEIVGKGFVMGNAPKDLQERIGNVTQDNDNDGIYYALVEYMEN"
     gene            11019..11879
                     /locus_tag="ACUBV3_000636"
     CDS             11019..11879
                     /locus_tag="ACUBV3_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HHU3847163.1"
                     /translation="MSYPYKANHSIESITLKVNDLENLVNFYSDIIGLTVIDKSSTRA
                     LLGVNQKIPLIILEKTELEKHSTYGLYHTAILVPDEYHLSLALNHLLSQHIPLEGGAD
                     HGYSNAIYLSDPEGNGIEIYNDKDISMWDIRESGQIIGITERLDIDNLLDSLVNVPNN
                     YKLSEKTSIGHIHLSVKDAKISSKLYQNVFGLDEKFAIPTASWIASGNYHHHLAFNNW
                     AGPNLSKNQEDRPGISLLTIAYNDDNLFRDSLKKAQLYQLTFLEKQDHYYIIEDFDGI
                     RIKVVLKPEN"
     gene            11922..12653
                     /gene="yaaA"
                     /locus_tag="ACUBV3_000637"
     CDS             11922..12653
                     /gene="yaaA"
                     /locus_tag="ACUBV3_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HHU3847164.1"
                     /translation="MIKILIPTAKEMKVCQNIAWPKLSAQTKIIIDYFSTLTVSDLED
                     IYRINTSAARCEAQRWQDFKAKQLTLNPAIKLFNSLMYRNIKRHNLSTSEAQFMENSV
                     FITSALYGIIPAMTLISPHRLDFNTKIKINNNSLKVFWRENYDTFMQSDDIMVSLLSN
                     EFETVFSPKERQKLIHLNFIEDRDGQLKTHSTISKKARGKCLTAMMENNCQTLEHLKQ
                     LRFDGFCYDNELSDSKQLTFVKKQT"
     gene            complement(13010..13627)
                     /gene="nrdG"
                     /locus_tag="ACUBV3_000638"
     CDS             complement(13010..13627)
                     /gene="nrdG"
                     /locus_tag="ACUBV3_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HHU3847165.1"
                     /translation="MSEEASWNTPKPGEWKSEELSHGHIIDYKAFNFVDGEGVRNSLY
                     VAGCMFHCKGCYNTATWSFRAGIPYTKELEDQIMTDLEQPYVQGLTLLGGEPFLNTGI
                     LLPLLQRIRRELPEKDIWSWTGYTWEEMMLETQDKLEMLSLIDILVDGRFDQSKRNLM
                     LQFRGSSNQRIIDVQKSLKEGEVVIWEGLNDGDNSYEQVKRDDLL"
     gene            complement(13700..14191)
                     /locus_tag="ACUBV3_000639"
     CDS             complement(13700..14191)
                     /locus_tag="ACUBV3_000639"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HHU3847166.1"
                     /translation="MKLRRPVLEDKEEILAMYKEFQKESSSVDGGFYEPTMHFEDWLD
                     HNLNMELGVGVPDNFVPYIQFVSFDNDNNAIGFLNLRLRLNDTLLEKGGHIGYSIRPR
                     QRGKGYAKEQLKLGIEQAHLKNINEILVTCHVDNDASKSVILANGGVLEDCLHQTERY
                     WIT"
     gene            complement(14200..15132)
                     /locus_tag="ACUBV3_000640"
     CDS             complement(14200..15132)
                     /locus_tag="ACUBV3_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HHU3847167.1"
                     /translation="MLQLGIVGLGGISQKAYLPYMRQVTGVHWHLFTRQKQILEEVNM
                     LFGSSTAYDSLDSLAEHPLDGVFIHVATSAHFDIAKLFLKKGIPVFMDKPLTEDYTST
                     KALYDLAKDHKTFLMAGFNRRFAPRIMEMKKVEDKNHIRTFKNAVNAPADFQYKLFDM
                     FIHPLDTALFLTNNVVKRGYFVTKRDGNKILQVSVTLETDSEIIEASMNLQSGSRREI
                     IEIESPEVTYSLDDLSNLSVIDGFDRRAIGFGSWASTLEKRGFEPMIDAFIQAITTGV
                     NPISPKSSLLSHFICDQINKANAPFGMLNLKIME"
     gene            complement(15145..15288)
                     /locus_tag="ACUBV3_000641"
     CDS             complement(15145..15288)
                     /locus_tag="ACUBV3_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3847168.1"
                     /translation="MGKYQLDYKGQAQVQKFHEKHSTGENANQKSRLKDLRKQFLEKA
                     KKK"
     gene            complement(15363..17561)
                     /gene="nrdD"
                     /locus_tag="ACUBV3_000642"
     CDS             complement(15363..17561)
                     /gene="nrdD"
                     /locus_tag="ACUBV3_000642"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HHU3847169.1"
                     /translation="MMVLERERFMTESDIKVIKRDGRLVSFDKYKIYTALLKASNKVI
                     KMSPLVEAKLEMIADHVIAEIYNRFKDNIKIYEIQNIVEHKLLEANEYAIAQEYINYR
                     TQRDFERSQATDINFSIGKLINKDQTVVNENANKDSDVFNTQRDLTAGIVGKSIGLKM
                     LPSHVANAHQKGDIHYHDLDYSPYTPMTNCCLIDFKGMLANGFKIGNAEVESPKSIQT
                     ATAQISQIIANVASSQYGGCTADRIDEFLAPYAQLNYQKHLKDAKEWVIEDKQEDYAR
                     AKTQKDIYDAMQSLEYEINTLFTSNGQTPFTSLGFGLGTNWFEREIQKAILKIRIQGL
                     GSEHRTAIFPKLIFTLKKGLNLEEDSPNYDIKQLALECATKRMYPDVLSYDKIIDLTG
                     SFKAPMGCRSFLQGWRDANGQDVTSGRMNLGVVTVNLPRVAMESNGDMDKFWEIFNER
                     MSIARDALVYRVERVKEAIPANAPILYQYGAFGERLGKYDNVDRLFNHRRATVSLGYI
                     GLYEVASVFYGGDWEDNHQAKAFTVDIVRKMKQLCADWSDEYDYHFSVYSTPSESLTD
                     RFCRLDTEKFGIVTDITDKEYYTNSFHYDVRKNPTPFEKLDFEKIYPETGASGGFIHY
                     CEYPVLQQNPKALEAVWDYAYDRVGYLGTNTPIDKCYQCQFEGDFTPTDRGFTCPNCG
                     NSDPKTVDVVKRTCGYLGNPQARPMVNGRHKEISARVKHMNGSSIKNQGN"
     gene            complement(17660..19201)
                     /locus_tag="ACUBV3_000643"
     CDS             complement(17660..19201)
                     /locus_tag="ACUBV3_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3847170.1"
                     /translation="MVENLEKPIGVSYKNSPSMSKRTAIRMKKSSRFSILLYSVLSTL
                     LAIANPLLTYFANGLQTQNLYTGLMMTKGQIPYSDVFATGGFLYYVTIALSYLLGSSI
                     WLLIVQFIAYYVSGIYFYKLVYYVAQSEIVSIGMTLIFYIMNIVLGFGGMYPIQWALP
                     FMLISLWFLIKFCVDNIVDEAFIFYGILAAFSLFIDPQTLIFWLCSFVLLTATNIKQK
                     QSLRGFYQFLCVVFGMILIAYTVGYFMFNLQIISSYIDKAIFYPFTYFARTNHSFLLS
                     LAIQIVVLLGSGCLFGLWDFIQNRKKASYQIGLNFIACIFIIYAIMAIFSRDFNLYHF
                     LPALPFGLLLTSNKITILYQKVIDRRSHRRQYFSGKSLIVDLFVKKTYYLPLLLVSLS
                     IGLLVYNTYQNVTLSKERRDISHYLTTKIDRDGKIYVWDKVASIYSQTRLKSASQFVL
                     PHINTAQKNNEKILKDELLQHGAKYFILNKNEKLPNELKSDIKKHYQEVPLSNITHFV
                     LYRFK"
     gene            complement(19247..19369)
                     /locus_tag="ACUBV3_000644"
     CDS             complement(19247..19369)
                     /locus_tag="ACUBV3_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3847171.1"
                     /translation="MSDTQLTLEEQEFLKQYMSRKYHYFRDIIRYGQVLAKLTK"
     gene            complement(19451..19768)
                     /locus_tag="ACUBV3_000645"
     CDS             complement(19451..19768)
                     /locus_tag="ACUBV3_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HHU3847172.1"
                     /translation="MAHNHNHDHNHEHQHEVITLVDENGNETLFEILLTIDGREEFGK
                     NYVLLVPAGAEEDEQGEIEIQAYSFTENADGTEGDLQPIPEDSDAEWDMIEEVFNSFL
                     DEE"
     gene            complement(19794..20213)
                     /gene="ruvX"
                     /locus_tag="ACUBV3_000646"
     CDS             complement(19794..20213)
                     /gene="ruvX"
                     /locus_tag="ACUBV3_000646"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003090938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HHU3847173.1"
                     /translation="MRIMGLDVGSKTVGVAISDPLGFTAQGLEIIKIDEESGNFGFDR
                     LAELVKEYKVDKFVVGLPKNMNNTSGPRVEASQAYGDKITELFNLPVEYQDERLTTVQ
                     AERMLVEQADISRGKRKKVIDKLAAQLILQNYLDRMF"
     gene            complement(20213..20479)
                     /locus_tag="ACUBV3_000647"
     CDS             complement(20213..20479)
                     /locus_tag="ACUBV3_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HHU3847174.1"
                     /translation="MGFTDETVRFRLDDSNKVEISETLTAVYRSLEEKGYNPINQIVG
                     YVLSGDPAYVPRYNDARNQIRKYERDEIVEELVRYYLQGNGIDL"
     gene            complement(20681..21079)
                     /gene="spx"
                     /locus_tag="ACUBV3_000648"
     CDS             complement(20681..21079)
                     /gene="spx"
                     /locus_tag="ACUBV3_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HHU3847175.1"
                     /translation="MIKIYTISSCTSCKKAKTWLNAHQLPYKEQNLGKESLTRDEILE
                     ILTKTESGIESIVSSKNRYAKALNCNIEELSVNEVIDLIQENPRILKSPILIDDKRLQ
                     VGYKEDDIRAFLPRSIRNVENAEARLRAAL"
BASE COUNT         6764 a         4306 c         3230 g         6854 t
ORIGIN      
        1 cctggttcac ctacatatgg acgagcgata atacgaccaa ggagagctgg acgctccatt
       61 gtgatactac gagcatattc acaaatccgg tagagttctt ctagagggat gatatcctca
      121 tgagctgcaa tttgtaggac tgggtcagct gaggtataga taatgagctc accagtttcc
      181 atttggcgtg gtccaaagtc atcaataaca gctgtccctg aataaggttt attcgcttca
      241 cgaataactt tacgtcctga aaaatcctca atctttgtga taatatcttc tggaaaacca
      301 ttccagaaag tatcaaatgg ctctgtaata tttaagccca tgatctccca atgccctgtc
      361 attgtatctt taccaagtga tacttcttgt aacttcgtcg cgtagccact tggattttct
      421 tctgcaggga cggtcttaag cgcttgggga cgaggaatat taccaagtcc aattttagcc
      481 atgtttggca ccgcaagtcc aactgttttt gaaatgtggc caagcgtgtc tgaagcacca
      541 tcaggaactc ctgcattgac aaaatcattg gcatctggtg ccgctccaat acccactgaa
      601 tctaagacaa ctaagtgaat gcgatcaaat tgtgacataa cttctccttt atcaattgaa
      661 aataggaagt cagaccagca tccttcctat ttcctttata ttatttaata agtgccttct
      721 tcaacttttt ggttcttaac aatcttgatg atacggctag tacccaagcg tgaagctcct
      781 aaagcaataa atttttcagc atcttccaat gatgagattc cacctgctgc cttaatttta
      841 acaccttcgc cgacatattt tgccatcact tcaacatctt caaatgtagc acctgctgtc
      901 gaaaaaccag tagaggtttt aataaagtct gcacctgaac gtgtgacaac cccacaaagt
      961 tcgataagtt cttctttagt taattgacat gtctcaacga taacttttaa gatatggtct
     1021 tgacatgcag ccttgatttg acgaatttct tcttcaacag tatcaaaatc cccattttta
     1081 acatctgtca aattaatgac catgtcaatt tcatcagcac catttttaat agcatcttga
     1141 cattcaaaaa ccttcgctgc agttgtacta tagccatttg ggaacccaat aacagtacaa
     1201 atagctaatt tacctgaaac gtactctgct gcttttttga cgtaagaggc tggaatacat
     1261 gctgaagctg tttcataagc cattgcatca tctaaaattg tttggatttc tggccatgtt
     1321 gctgttgttg ctagcaaagt atggtctacc gtttttaaaa tatcttttac ttccacgtta
     1381 ctatccttct ttcttttgtc aacttttaga atagagttac aataccgacg ataaaggcgc
     1441 taagcatact tacagcaata ccaccaatca ttgcacggaa aacaagtcga gctagggtgc
     1501 cacgtttctc tggacaaaga acagcaatac ctgaaacaca aatacctaaa cttgataaat
     1561 tagcaaaacc acaaagtgaa atagttgcta ccaatgctgt acgataatct aaagatttga
     1621 ttaggtcacc caattgttgg aacgaaacaa actcatttaa aatcaactta cttccaagaa
     1681 ggtttccttc tagaagaatg tttttgtggt caaatcccat aagaaaacca aatggagcaa
     1741 aaacatatga gaagatttgt tctaagcgga ttcccaatcc acttaacatc atattaatca
     1801 aagaaactaa accaacaaag gcaatcaaac tagcaccaat tgagaaagcc atttgtgcac
     1861 ctgtgcttgc accctcagcg attgcatcaa tcacattggc gttattacct ttattatcca
     1921 tcttaatgtc atcaattttt tgaacaggtt ctgtttgagg caataagatt ttagcaatga
     1981 gaatactgcc aataggaacc attgttgaag caatcaagag atattccatt ggaatgccta
     2041 atgcaatgta gccaccaaga atagaaactg acatacttcc catacctgat accaacacaa
     2101 ccattatctc actatcagtc atacgaccta ggtatttgct aaccaaaatt ggactgtctg
     2161 tttgaccaag aaacatatta gctacggcaa caaaactctc aacctctgag gatttcataa
     2221 ttttaccaac gcccttacct atccatttta ctacaaatcc aaggattcct acataataaa
     2281 gtagactaac tagggcagat aagaaaacaa tattgccaag cgtttgaatg gcgaaaataa
     2341 aaccagtttt tgctccacta tctgctaatg aaccaaacac aaaatttaat ccagcttgac
     2401 cacagttgat tactttagta actccagttg aaacaacact aacaacttgt tgacctagtg
     2461 ggatacgtac taagattagc gcaataatga attgaacgat aagagctttt ccaattaaac
     2521 ttagagaaac actcttacga ttgaaagaaa ttgcatacac aattcctaat accaataaaa
     2581 taccaataat actataaata aattgcatga tgacttccaa aacctcgctt attgtgattt
     2641 atcattttca ataagtgtgc ggagtgcttc aacagcaact tgaatagctg cttctgtgtc
     2701 atgagccatt gggttatcca tacccaaagc gttgcgttct tgattaccaa caacaaggaa
     2761 atctgatcca caacgaactc ctagatgact tgctgctaca aaaagagctg cagattccat
     2821 ttcagacgct tttgttccca gacgtttcca tgcttcccat ttatttaata attcataact
     2881 aactggcata cgttctggct catgttgtcc ataaaatgca tctttacatt gaactactcc
     2941 tgcgtgactt gtgtaaccaa gctttttagc tgcattaact agagcattcg ttacctccaa
     3001 gtcagctaca gcaggaaatt caatcggagc atattcttta cttgtacctt ccatacggat
     3061 agctccagtt gcaataacga tgtctccgcc tttaacatca aggtcaatac caccacatgt
     3121 tccaacacgg ataaaggtat cagcgccaca gagtttcaat tcttccatcg caatagacgc
     3181 tgatggacca ccaataccag ttgaagtaac actgactttt tcaccgttta atgtacctgt
     3241 ataagtaaca tattcacggc tgtctgctac aagaactgca ttatcaaaat gttctgcaat
     3301 ttttgcacaa cgttttggat cacctggcat gataacataa cgacctacat cacctggacg
     3361 aatctgtagg tgatattgca atccgacttc acctgaataa ttctgcatga taaagacctc
     3421 cttaatgtct tgtctatttt tttacacttg tataataaca tacaagacaa cctttgtaaa
     3481 cgcttttatc gaaagaaaac gttttatttt ttaacttgat ttttctaatt ttttcaatta
     3541 aactaacttg tatacggagg ttacaatgcc taaaaatgaa ctaaataata aactaaataa
     3601 actaaagcat gtacaagtct acaataagat ttttaacatg attcaagacg gtacttattc
     3661 tcctggaatg caattacctt ctgaacccga attagcaggg caacttaatg ttagtcgagc
     3721 tactttacga aagtcattag cactattaca agaagaccat ttggttaaaa atatcagagg
     3781 aaaaggtaat ttcattagag aaaattctag caacttgtct gaaaacggtt acgaaaatag
     3841 acaacaccct attaaaactt gtctaacttc caaaattaca gaagtagaat tagaatttcg
     3901 tgtagaggtt cctgcagaag ctatcacagc ttcacttaaa caagaaaccc ctgtagttgt
     3961 tattgctgat cgttggtacc acaccgatga tggaccatta gcttacacac tttcttttat
     4021 ccctatcgaa cttatttcag atgctgagat ttctttacat gatactaaac agttgttaaa
     4081 cttcattgaa gaaggtatct atcaagaagg catatcaagc cactcacaaa gccatttggg
     4141 atatgcgaca tctggcaatt tttcagcaac taaatatacc ctatccgatc atggacaatt
     4201 catattaata caagaaacaa tttttaagca agagaaaata ctaatgtgta ataagcatta
     4261 cgttcccatt gagcactttg aattgtccat tacctctcat taaggaggca agcctatgca
     4321 aaatagacta ttaaaagttg cttttttcat aacttttacc gaattaatta tgggcctaat
     4381 agttggcgct tattttaagt taagttattt tctagcccct ccaaaggaac cgtcaatggg
     4441 tgctgggtac ggaatgctct tttctctgtt atttatactt attagttact taattctatt
     4501 agctataatt caattcttag cttatcaatc tcaatctaag ataatagcaa tcattgtttt
     4561 gctacttttt tcctcccact actggcaaat attagatggc ctatccttat gatcttctta
     4621 ccgcaagtgg tagtgactat catatctcta ttatcacaac ataaaactcc cctttgttaa
     4681 aggggagttt tatgttgtga taagttagtg attagaagcc acccatcata gatggatcca
     4741 ttgcaggagc tgtaggagct tctggttcag gtttatttgc tactactgct tctgtagtca
     4801 agataagact tgctacagat gccgcatttt gaagtgcaga acgtgttact ttgacagggt
     4861 caatgatacc tgtggtaacc atatctaccc attctccatt ggccgcatta aatcctgtac
     4921 caatttcaga ctgttttaaa cgttcaataa taactgaacc ttcatatcca gcattgtaag
     4981 caatttgacg aacaggctct tcgagagcac gaagaacaat attacgtcca gtctcctcat
     5041 caccattaag tttcagtgcc gctacttttt caataacgtt cacaagagcc gtacctccac
     5101 ctgaaacaat accttcttca actgcagcac gcgttgcatt taacgcatct tcgatgcgaa
     5161 gtttcatctc ttttaattct gtttcagtcg ctgcaccaac tttaattact gctacaccac
     5221 cggctaactt agcaagtcgt tcttgtaatt tttcacgatc aaaatcagaa gttgtagcct
     5281 ccatctgtga cttaatgata gctacgcgat tagcaattgc tgatgagtca ccggcacctt
     5341 cgacaataac agtagaatct ttatctactg ttactttagc agactgtcct aaaacttgca
     5401 tagtagcatc ttttaagtct aaaccaaggt cttcagtaac gacagttcct cctgttagga
     5461 tagcaatatc ttccagcatg gctttacgac gatcaccaaa tccaggcgct ttaacagcta
     5521 cgacattgaa agttccacga attttgttaa gaacaagcgt tgggagagct tctccatcaa
     5581 catcatcagc gatgattagc aacggacgat ttgttttaag aacctcttct aataatggta
     5641 aaatttcttg gatatttgaa attttcttat ctgtaataag gatatacgga ttctcaagtt
     5701 cagagaccat tttctcgtta tcagttacca tatactgtga caagtaccca cggtcaaact
     5761 gcattccttc cacaacttca agctctgttt ccatacctcg cgattcttca atagtgataa
     5821 caccatcatt acccacgcgc tccatagctt cagaaatgta ttccccaact ttttcagaac
     5881 gtgaagacac agccgcaact tgagcaatag cttctttgcc tgaaactggt tgtgcaatct
     5941 cttttagctc ttcaactgct gctgaaacag ctgtttcaat accacgacga atgccaatcg
     6001 gatttgcccc tgcagttaca tttttaagac cttcccgtac aatagcttgg gtcaaaacag
     6061 tagcagttgt agtgccatcc cctgcaatat cattagtttt tgaagccact tctgacacaa
     6121 gtttagctcc catattttca aagtgatctt ctagctcaat ttctttagca attgtcacac
     6181 catcatttgt aattaaagga gaaccaaatg ctttttcaag aacaacatta cggcctttag
     6241 gacctaatgt tactttgact gtatcagcta aaatatcaac accacgcacc attgctgagc
     6301 gggcatctgc tgaaaattta atatcttttg ccataattta atacccaggc ctaaaattta
     6361 acaaagtcta ctttctttac aaagcaactt ttctctaata ggcctatatc ctttcttgct
     6421 taataaaacc tatttaacga cagcaacaat gtcactttcg cgaacaactg tcactttttc
     6481 gttaccgtct ttaacttcca gaccagcacc attttcgact aaaacagtat caccctctgc
     6541 aacgctcggt gcaactaatt cccctgttaa ggtacgaata ccgtcaccca cagcaagaac
     6601 tttggctgtc tttgtcgcat catggcttgc tcctgcaaga acaaaaccac cgacagattt
     6661 ctcttcagtt tcaacaaacg aaatgatcac acgatcacct aatggtttta acataataaa
     6721 cgaacctcca tacgagatat attttgattt tttagcactc ctatacagag agtgctaact
     6781 acaaaaacta ttctatcact tggtcagaat tagtcaagag aaaaggcacg tttcaaacaa
     6841 gaaaaatttc cctagttatc ctgtccattc actagagaaa tttatatttt aacctaagac
     6901 aagggcatca tctatcaact gttgacctga gtttttatgg aaaagttcca ttaattctgc
     6961 aactgttaag tttctctttg cttctccttt aacatcaaca acaattttcc cgtggtagag
     7021 catcacgaga cgattaccat aagcaatggc atgctccata ttgtgcgtaa tcattaaggc
     7081 tgtcaatcgt tgttcttcta tcactttctg tgttaattcc ataaccatat cgctagtttt
     7141 gggatctaga gccgctgtat gttcatccag taataataat ttgggacgaa caagagttgc
     7201 catagctaac gtgagggctt gacgctgacc gccagataaa aatgcagcat cagtcttcat
     7261 tcgattttct aagcctagac ctaattgtga tagagacttt ttaaatagtt gacggtcatc
     7321 gtcagtcact gactgtctaa aaatgtgacg cttattgcct cttttatgtg caattgccat
     7381 attttcttca atcgtcaaat ttgttgcagt tcccattcta ggatcctgga agactcgact
     7441 aatatcctta gatcgttttt ctacagagtc tttagtaatt gattggttgt ctaatgtgat
     7501 tgccccctga tcaataggga tcagaccagc gatacagtta aggagggttg attttcccgc
     7561 accgttacct ccaattactg aaataaaatc accgtcttca atcgttaaat ctaaaccacg
     7621 taaaacatgg ttctcattga cagttccttt ttcaaaagtt ttgtgcagat tgactaattc
     7681 aagtaatgac atgttcttat ccttcctttg taagtgattt agaggttttt actcctagtt
     7741 tagctcgtaa ttctgggaca tacaaaatga gagccaacaa aattgctgaa accaacttta
     7801 tcatctgtgc atcaatatcg gttgttaata tgaaaacaat aatcatacgg tataaaacag
     7861 accctaatac aattgaccat aaccttttgc caagaggtaa gtattttatc attacttctg
     7921 ccaagataat tgaagctaat ccaataacga ttgtacccac tcccatattc aaatctgcat
     7981 agccattatt ttgcgctaaa agtgcaccag ataaagcaat caggccattc ccaatcatat
     8041 atcctagcat tttcatccga tctactttaa tactattggc ttgtcccata gcctcattat
     8101 cacctgtcgc tcttaaagct aatcctagtt gggtatttaa aaagagatac aaaattaaga
     8161 taaccaagcc tacacaaaca attccaatta ataaaactgc tgaaagttta tttaaaccta
     8221 atcttgttac cattgtaacc aaagtatttt tgagagcaaa agagacgttt gatcttccta
     8281 aaacaagtaa attaatagag taaagtcctg tcaacgtaat aatacctgtg aggagggctg
     8341 gtattttcat tttagtatga agaaaacccg atactagtcc tgccaacata ccacctaaca
     8401 tgcccgctat ggttgccaca attggattaa tatcattaac gatacataaa gcgcaaactg
     8461 cagcccccat agggaaagca ccctctgcag agagatcggc tatgtctaag atacgaaaag
     8521 tgataaacac gccaattgcc ataattgacc aaagtaatcc ttgcgaaatt ccggataaga
     8581 taatatctaa catacaatac ctttctgatt atttattatt tttaatactt gaaacatcaa
     8641 ttccaagctc ttttgccata tcttcattaa ctactacttt tagattttga ggatattctg
     8701 aaggaacttt agcgacgtct ttacctttga ggattttaac agcttgtctt gccgtttgac
     8761 gacctaaagc ctcataatca gccccataag aaaagagcaa accacttggt accatatctg
     8821 ctgaacctcc cacaacaggc acttttaatt ctttcgatag attccctaac agcgttactg
     8881 agctggctat aatattatct gttggtacaa aaataacctc tgttttagac attaaactct
     8941 tagcagtatc ttgtacatca ttagtggaag aaataccttt aacacttgtt ttaatccccg
     9001 cctcttggaa gatttttttt gcttgtttta cctgaacttc tgagttacgc tcgctagtgg
     9061 tatacataat accaacacgt ttaaccttag gcattacttt tctcaaaagg gatacttgct
     9121 ttttaattgg agacatgtca cttgtaccag tcgctaaacc ttcaggtttt ttcattgatt
     9181 ttactaattc ggctgatacc ggatctgtca ctgctgtaaa taaaataggt gtttctgttg
     9241 acaccgttgt caacgattga gccgccggag tcgcaattcc caatactaat ttattatctt
     9301 taatgagttt ttcagaaatt gattggatat ttgactgatc cccttgagca ttcttatact
     9361 caattttgat atttttgcca tctttatagc cttctttagc taattctttt ataaatcctt
     9421 tcctagtggc agttagagag ggatgttcta catattgtaa aataccaata tgttttacat
     9481 cagattgacc attttgagaa gattgtgaac aagctgtcat cagaacagtt gctaaaacgc
     9541 tgctaattac catctttgtt atttttttca ttactatctc cttgtaatac catcaaattt
     9601 tccataaaac aaaaaaccaa ctagacccta catctagttg gttaattatt tctctcaatt
     9661 cctctgtgca cgggagggcg aaaaaatatt gccttaacca actagattat tgaaatgatc
     9721 taatcggctc tgctttcatc ataaaatgct ttagcttcat agatacaaac tttgcgtgac
     9781 gtttgtatcc ttgaggctac aaacgtctat ctaaagaaac taaagtaata ataagtattt
     9841 gtttgtttaa taatagacga tttgattaac atattattct ctccttgttt atggttgcct
     9901 ataagtttac gtcatttaaa aataattgtc aatagttaaa tttgattttt ttgaaaattc
     9961 ttgttagata gataatttaa ataatagtca ttcgttgata taacacgctt ttaaggaaat
    10021 ttagatttgc tatccttaat tttccatata ctctactaaa gcataataaa taccatcgtt
    10081 atcattatcc tgagttacat ttccaattct ctcttgtaaa tctttaggag cattccccat
    10141 gacaaaacct ttacccacaa tttcaagcag atttaaatca ttatagttat caccaaacgc
    10201 aatagagtct gcaagagaaa tatctaaata gtctgctaaa gttttagcag attttccttt
    10261 ttcaatacct ggagccatta tttcaataaa gtaaggagca gattgtgcga ttgataaatg
    10321 tggatattga gctttcaaag tgttctccaa ttctcccatc agagatggtt ctcccatcaa
    10381 gagaagttta tgaatcctat cttgcttctc taactccatc aaactcatct ctttagattg
    10441 taaaccaaca atctcctcct ctttttggac ccactcattt tctttatcac aggaatacca
    10501 ttcatgataa cgatagacat tccatgcaat tttcgaataa tgcttgctta cataagaaca
    10561 aacttgtaag gcaacttgac catccattgt gtgggaagca attgtttttt gttgcatatc
    10621 ttgtattaaa gccccattat aggaaataat ataagaatct attccaattt gttccataat
    10681 cggagtaatt gcctcaggca tcctagctga tatgggaacg aaagtagccc cttgagctac
    10741 tatttgttta attccctgcc gtgtcttagg tgttacttta aaattactgt taattaaagt
    10801 accatcaata tcactaaata ttagccttac catattatcc tccaaaatat aattgtacac
    10861 gcttacttta gtctaacact taaatttttg tttcgcaaat aaacttcgtc tggtctaatg
    10921 agactactaa tataagctat atttcaaaac aaattagttt atttctctca atgaacggtg
    10981 taatatgtta ctatatagta ataataggag gattaactat gtcgtatcca tacaaagcca
    11041 atcattccat tgaatcaatc accttaaaag taaatgacct cgaaaacctc gtaaacttct
    11101 atagcgatat cattgggtta actgttattg acaaatcaag cactagagcc ttactagggg
    11161 taaatcaaaa aatccctctt attatccttg aaaaaactga acttgaaaag catagtacct
    11221 atggccttta tcacactgct attttagtac cagatgaata ccatctcagt ctagctctaa
    11281 atcatttatt atctcagcat atccctctag agggtggagc tgaccatggc tatagcaatg
    11341 ctatctatct aagtgatcct gaaggtaatg gaattgaaat ttataatgat aaggatatct
    11401 ctatgtggga tatcagagag agtggacaaa ttattggtat tactgaaaga ctagatattg
    11461 acaatttact tgacagcctt gtcaacgttc ctaataatta taagctttct gaaaaaacta
    11521 gtattggtca tattcatttg agtgttaaag atgctaagat tagctcaaaa ctctatcaaa
    11581 atgtttttgg ccttgatgag aaatttgcaa tcccgacagc tagttggatt gcatccggca
    11641 actaccatca tcatctagcc tttaataatt gggctggtcc aaatctttct aaaaaccaag
    11701 aagatagacc cggaatttcc ttattgacaa ttgcttacaa tgatgacaac ctttttagag
    11761 atagcttaaa aaaagcgcaa ctttaccaac tgactttcct tgaaaaacaa gatcattatt
    11821 atattattga agattttgat ggcatccgaa ttaaagttgt tctaaaacca gagaattaac
    11881 catcacttga cttttgcctc ttcattttac ataatgtcaa tatgataaaa attttgatcc
    11941 caactgcaaa agaaatgaaa gtgtgtcaaa acattgcatg gcctaaactg agtgctcaaa
    12001 cgaaaattat aattgattat ttttctactt taactgtttc agacttagag gatatctaca
    12061 gaattaatac aagtgcagct cgttgtgagg ctcaaagatg gcaagatttt aaagctaaac
    12121 aacttactct gaatcctgct attaagttat tcaacagtct tatgtaccgc aatatcaaaa
    12181 ggcacaactt atccacaagt gaagcacaat ttatggaaaa ttctgttttt attactagtg
    12241 ctctttatgg catcattcct gctatgacac taatatcccc tcatcgttta gattttaaca
    12301 ctaaaatcaa aatcaacaat aactctctta aagttttttg gcgtgaaaat tacgatactt
    12361 ttatgcagtc agatgacatt atggtttctt tgctttctaa tgaatttgaa actgtctttt
    12421 ctcctaaaga gcgtcaaaaa ctaatacatc ttaactttat tgaagatcga gatggccaat
    12481 taaaaaccca ctctaccata tcaaaaaaag caagaggaaa gtgtttaact gctatgatgg
    12541 aaaataattg ccaaactcta gaacacttaa agcaattacg ctttgatggc ttctgttatg
    12601 ataatgaact ctcagactct aagcaattaa cttttgttaa aaaacagact taagtaacta
    12661 tttatccaca atttattcac acaaaaagca agagataatc gtatctcttg ctttttgaca
    12721 aacttgtaag gtctaaaaac ctctctatat caaggtttca aacaatatta acaaaaaata
    12781 attgttatta acaacttgtg gataactatg gggatagaca gaggataatc tgtttatata
    12841 aagttgatta cttaacacta atgtcattgt atataccttt agatttcaaa aatacttgtt
    12901 aaattaacaa taataaacgt caacatcttg tcacagactg tcaaatactt ataaacaagt
    12961 tgtggataaa cataactttt aatttttatt taaaagttaa tttttgacac tagagtaaat
    13021 catctcgttt gacttgttca taactattgt caccatcgtt taatccttcc caaatcacga
    13081 cttcaccttc ttttaaagat ttttgtacat caatgattct ttgatttgaa gatcctctaa
    13141 attgtagcat taaatttcgc ttactttgat caaacctacc atccactaaa atatcaatca
    13201 aagataacat ctccaattta tcttgagtct ccagcatcat ttcttcccaa gtataccccg
    13261 tccatgacca aatatctttt tctggcaatt cacgtcggat acgctgaaga agtggcaaca
    13321 aaatacctgt atttaagaac ggctctccac ctaataacgt taaaccttgg acgtaaggtt
    13381 gttctaaatc tgtcataatt tgatcttcaa gctcttttgt ataagggatt ccggctcgaa
    13441 aggaccatgt agcagtatta taacaccctt tacagtggaa catacatcca gctacgtaga
    13501 gtgagtttct aacaccttca ccatctacaa aattaaaagc tttataatca atgatatggc
    13561 catgacttaa ttcttcgctt ttccactccc caggctttgg agtattccaa cttgcttctt
    13621 ctgacatttc taacctctct attgatatga aaagaaccag aactaactgg tctttttatt
    13681 attatcttaa agtactattt taggttatcc aataacgctc tgtctgatga agacaatctt
    13741 ctaaaacacc accgttagct aatattactg acttgctggc atcattgtct acatggcatg
    13801 tcacaagtat ttcattaata ttttttagat gtgcttgctc aattcctagc ttcaactgtt
    13861 ctttagcata acctttgcca cgctggcgag gtcgtatgga ataaccaata tgtccacctt
    13921 tttctaaaag agtatcattt agtcttaaac gtaaatttaa gaaacctata gcattattgt
    13981 cattgtcaaa agacacaaac tgaatatatg gaacaaaatt atcaggaacg cctaccccta
    14041 actccatatt taggttatga tctagccagt cttcaaaatg catagttggt tcataaaaac
    14101 caccgtccac tgaactactt tctttttgaa actctttata catggctaaa atttcttctt
    14161 tatcttctaa cactgggcgc cgtaatttca tattgtttcc tattccatta tcttcaagtt
    14221 aagcattcca aaaggagcat tcgctttatt aatctggtca caaataaaat gactcaataa
    14281 actagacttt ggtgatatag gattgacacc tgtagtgata gcttgaataa aagcatcaat
    14341 cattggttca aaaccacgtt tctctagagt gcttgcccaa gaaccaaaac cgatagcacg
    14401 acggtcaaag ccatcaataa cagaaagatt tgacaaatca tctaatgaat aggttacttc
    14461 tggcgactct atttcaatta tttctcgacg agaccccgac tgcaggttca tgcttgcttc
    14521 tattatctca ctgtctgttt ctagcgttac tgatacttgt aatatcttat tcccatcacg
    14581 tttagtcaca aagtatcctc ttttaacaac attatttgtc aaaaatagcg ccgtatctaa
    14641 aggatggata aacatatcaa aaagcttata ttgaaaatcc gctggtgcat tgacagcatt
    14701 tttaaaggtg cgaatatggt ttttatcttc tacttttttc atttccataa tacgtggtgc
    14761 aaagcgacga ttaaaacctg ccattaaaaa tgttttatga tccttcgcca aatcatacaa
    14821 tgctttagtt gaagtataat cttctgtcaa aggcttatcc ataaaaactg gaataccctt
    14881 ttttaaaaaa agctttgcta tatcaaagtg agcactagtc gcgacatgaa tgaagactcc
    14941 atccaaagga tgctcggcta gactatctaa actgtcataa gctgtagaag aaccaaaaag
    15001 catattaact tcttctaaaa tctgtttttg gcgtgtaaaa agatgccagt gcacacctgt
    15061 cacttgacgc atataaggta gataagcttt ctgtgaaata cctcctaaac cgacaattcc
    15121 aagttgcaac atagcatcct ccttctattt tttctttgcc ttttccaaaa attgtttcct
    15181 taaatctttt agacgtgatt tttgattagc attctctcca gtagagtgct tctcatggaa
    15241 cttctgtact tgtgcttgcc ctttgtaatc taattggtat ttccccatat cttaataact
    15301 ccttctattt tatcttctta ttttaacata taatagacca gaggttttaa tagaggtttt
    15361 aattaattac cttgattttt aatacttgat ccgttcatat gttttacacg agcagagatt
    15421 tccttatgac gaccattaac catcggacga gcctgtggat tacctaaata accacatgta
    15481 cgtttgacaa catctactgt cttagggtcg ctgttaccac aatttggaca agtaaaacct
    15541 cgatctgtcg gtgtaaaatc cccttcaaat tgacattggt aacacttatc aatcggtgta
    15601 ttagtaccta gataacctac ccgatcataa gcatagtccc aaactgcttc taaagctttt
    15661 ggattctgtt gcaaaacagg gtattcacag tagtgaataa atcctcctga tgcacccgtt
    15721 tcaggataga ttttctcaaa atctagtttt tcaaaaggtg ttggattctt acgcacatca
    15781 taatggaatg aattagtgta gtattcctta tcagtaatat cagttactat accaaatttt
    15841 tcagtatcta aacgacaaaa ccggtcagtc aagctctccg atggtgtcga ataaactgaa
    15901 aaatgataat catactcatc agaccaatct gcacataact gcttcatctt acgaacgatg
    15961 tctacagtaa atgctttagc ttgatggtta tcttcccagt caccaccata aaaaacactc
    16021 gccacctcat ataaaccaat ataaccaaga gacaccgttg cacggcggtg attaaagagt
    16081 cgatcaacat tgtcatattt tcctaaacgt tctccaaatg ctccatactg gtaaagaatg
    16141 ggcgcatttg ctgggatagc ctctttgaca cgttccacac gataaaccaa ggcatctcta
    16201 gcaatactca tacgttcatt aaatatctcc cagaatttat ccatatcacc gttagactcc
    16261 atggcaacac gcggtagatt aactgttaca actccaagat tcatacgacc agatgttaca
    16321 tcctgaccat ttgcatctcg ccacccttgt aggaaagaac gacagcccat aggggcttta
    16381 aaagagcccg ttaagtcaat aattttatca taagaaagga catctggata catccgtttt
    16441 gtcgcacact ctaatgctaa ttgtttaata tcataattgg ggctatcttc ttctaaatta
    16501 agaccttttt taagcgtaaa aattaatttt ggaaaaatag cagtacgatg ctcgcttcct
    16561 aaaccttgaa tccgaatttt caaaatcgct ttttgaattt ctcgttcaaa ccaattagtt
    16621 cctaatccaa aaccaagcga agtaaatggc gtttgcccat ttgaagtaaa taaagtatta
    16681 atttcatact ccaaagattg catggcatca tatatatctt tttgtgtttt agcgcgcgca
    16741 tagtcttctt gtttatcttc aataacccat tcttttgcat cttttaaatg tttttgataa
    16801 tttaactgtg cataaggagc taaaaactca tcaatgcgat ctgctgtaca gccaccatat
    16861 tgacttgaag caacgttcgc aataatctgt gaaatctgag cagtcgctgt ctgaatagat
    16921 tttggactct caacttctgc attaccaatc ttaaaaccat ttgcaagcat tcccttaaaa
    16981 tcgattaaac agcaatttgt catgggagtg tatgggctgt aatctaaatc atgataatgg
    17041 atatcaccct tttgatgtgc atttgctaca tgagaaggta acattttcag accaattgat
    17101 ttccctacaa tacctgctgt taaatcacgt tgggtattaa acacatcaga gtctttatta
    17161 gcattctcat taacaacagt ttgatcctta ttaattaatt ttccaataga aaaattgata
    17221 tctgtagcct gtgaacgctc aaaatcgcgt tgggtacgat aattgatata ttcttgtgca
    17281 atagcgtact cattagcttc caataattta tgttcaacga tattttgtat ttcatatatc
    17341 ttgatgttat ctttaaaacg attgtagatc tcagcgatga catgatctgc aatcatctca
    17401 agtttagcct caactaaagg agacattttg ataactttat tactagcttt taatagagcc
    17461 gtgtagattt tatacttatc aaagctgacc aaacgcccgt cacgtttaat cactttgata
    17521 tcagattctg tcataaatcg ttctctttcc aataccatca tgtgaaatat cctccagtga
    17581 caattatagc atgcaaagaa aaaaaatcaa tatattgtgt caagtttttt gctcagacac
    17641 aatatattga ttttttaatc tatttgaaac gatatagaac aaaatgagtg atatttgata
    17701 gaggaacttc ttggtagtgc ttcttaatat cagatttcag ttcatttggt aacttctcat
    17761 ttttatttaa gataaagtat ttagccccat gctgaagcag ctcgtccttc aaaattttct
    17821 cattattttt ttgcgctgta ttaatatgtg gcaaaacaaa ttgagaagca gattttaacc
    17881 gagtctggct ataaatgcta gcaactttgt cccaaacata aatcttacca tctctatcaa
    17941 tttttgtagt caaataatgt gaaatgtcac gcctttcttt actaagagta acattttgat
    18001 aagtattata aactagtaac cctatcgata aggaaactag taagaggggc aaataatatg
    18061 ttttctttac aaaaaggtct acgataagag atttaccaga gaaatactga cgtctatgtg
    18121 aacgtctgtc aatcactttt tggtaaagaa tagtaatttt attactcgtt agcaaaagac
    18181 caaatggtaa agcaggtaaa aagtggtaaa ggttaaaatc tcttgaaaaa atggccatta
    18241 tagcgtaaat aataaaaata caggcaataa agttcaagcc gatttgataa gatgcttttt
    18301 tacgattttg aataaaatcc catagtccaa agagacaacc tgaccctaga aggacaacaa
    18361 tttgaattgc taaactaaga aggaatgagt ggtttgtcct agcgaaatac gtaaaggggt
    18421 aaaaaatagc tttgtcaata taagaagaga taatctgtag attaaacata aagtaaccaa
    18481 cagtataagc aattaaaatc ataccaaaaa caacgcataa aaactgataa aatcctcgta
    18541 gtgactgctt ttgcttaata ttggttgctg ttaagagaac aaaagagcaa agccaaaaaa
    18601 tcagtgtttg gggatctata aatagtgaga atgctgctaa aatgccataa aatataaatg
    18661 cttcatcaac gatattatcg acacaaaatt taattaaaaa ccatagcgaa atgagcataa
    18721 aaggtaatgc ccactgtatt gggtacatac caccgaatcc taagacaata ttcattatat
    18781 agaaaatcaa cgtcatgcct atcgagacaa tttcactttg tgccacataa taaactaatt
    18841 tataaaaata aattccagat acatagtaag caataaactg tacaataagt aaccagatac
    18901 tagaccctaa aaggtaactt agagcaatcg ttacatagta taaaaatcct cctgtagcaa
    18961 aaacatcact ataagggatc tgtcctttgg tcatcatcaa acctgtatag agattctgag
    19021 tctgcaaacc atttgcaaaa taagtcaata aaggatttgc aattgctaag agagttgaca
    19081 aaacactata tagtaaaatt gaaaatcgac tagatttctt cattctgata gccgttcgtt
    19141 tactcattga tgggctattt ttatagctca ctccaattgg tttttctagg ttttctacca
    19201 ctatttttct cctcatattt acatactacc agtatatcag aaaaccttat ttagtcaatt
    19261 tagccaacac ttgaccgtaa cgaataatat ccctaaagta gtggtatttt ctagacatat
    19321 attgtttgag aaattcttgt tcttctagtg ttagctgagt atctgacatt aacatcacct
    19381 ccttttacta catcatcact attattattc gaaaaaaaaa cgactgaaca attgctcaat
    19441 cgcttcgata ttattcttca tctagaaagc tgttgaaaac ttcctcaatc atgtcccatt
    19501 ctgcatctga atcttctggt ataggctgaa ggtctccttc agttccatcg gcattttctg
    19561 taaatgagta agcttgaatc tcaatttcac cttgttcgtc ttcttccgca cctgcaggaa
    19621 cgaggagaac atagtttttg ccaaactctt cacgcccatc aatagttaat agaatttcaa
    19681 aaagtgtttc gttcccattc tcatctacaa gagtgattac ttcatgttga tgttcatggt
    19741 tatgatcgtg gttatgatta tgtgccattg tttctccttt ttatgccgaa tgcttaaaac
    19801 atgcggtcta aataattttg taagattagt tgagctgcta atttatcgat aactttctta
    19861 cgcttaccac gactaatatc agcttgctca actagcatac gctctgcttg aacagtcgtt
    19921 aaacgttcat cttgatattc aactggaaga ttgaataatt cagtgatttt atcgccatat
    19981 gcctgactag cctctacacg aggaccactt gtattattca tatttttagg tagaccaact
    20041 acaaatttat caactttgta ctcttttact aactctgcta agcggtcaaa accaaaattt
    20101 ccagattctt catcgatctt aatgatttca agaccttgtg cagtgaaacc gagtggatca
    20161 ctaattgcaa cgccgacagt tttcgagccg acgtcaagtc ccattattct cattacaaat
    20221 ctattccatt tccttgtaaa taataacgaa cgagctcttc tacaatctca tcacgttcat
    20281 atttacgaat ttgattgcga gcatcattgt aacgaggtac ataagcaggg tccccactca
    20341 atacgtaacc aacaatttga ttgatgggat tgtaaccttt ttcttctaat gaacgataaa
    20401 cagctgttaa tgtttcacta atttctactt tgttactatc atctaatcta aaacgaacag
    20461 tttcatctgt aaatcccata cctacacctt ctttccttta attctttcac ttattataac
    20521 ttaaataaag tatttgtaca agaaattgat aaacactata ttagaaaatc aaaaagggag
    20581 cacaagaaac caagaagtga ttctttgatt tctttactcc ctaccttata ggtgattttt
    20641 acaggttatc gtgtgactct cacttatgct tatctaaagc tcaaagtgct gcacgaagac
    20701 gagcttctgc attttcaacg ttacgaatag agcgaggtag aaaagcacga atatcatctt
    20761 ccttataacc tacttgaaga cgtttgtcat caattagaat tggactcttt aaaatacgtg
    20821 gattttcttg aataagatca ataacttcat ttacactcaa ctcctcaata ttacaattga
    20881 gcgccttagc ataacgattt ttagaagaaa cgatactttc aataccactt tcagtttttg
    20941 ttaatatttc taaaatttca tcacgtgtta gtgattcttt acctaggttt tgttctttat
    21001 atggaagttg atgagcattt agccaagttt ttgctttttt acaacttgtg caacttgaaa
    21061 tagtataaat cttaatcatg tgccttctca ctttctatcc catgatagtt atagtttaac
    21121 atagtcacaa aaatcaaaca tgggacttaa gacc
//
LOCUS       DBJYSH010000021        21029 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.021, whole genome shotgun sequence.
ACCESSION   DBJYSH010000021 DBJYSH010000000
VERSION     DBJYSH010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 21029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21029
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.021"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            <1..755
                     /locus_tag="ACUBV3_000649"
     CDS             <1..755
                     /locus_tag="ACUBV3_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HHU3847176.1"
                     /translation="GSALPLKQLKLGNFAPWNQLFEVSNSTYILIVLFTLFLSAPLFS
                     GDRAKKINPILLTTRYGRGQLTWAKLASTMTIGISTFILAYALILTVFADYFGFSGWE
                     TSVQLNLYWTSPLANIMGFPVPMTISTAFVHIIVLQFVGLLFIASLNFFISSITNSPL
                     TSFAVSTLAFFAPSFLMAMFNKGVINKLLTIFSVSTTDTSASLLKLSRNGSHGFFFDS
                     FSANEICLISLRMLIAVLLIAYTYRLMRRRSL"
     gene            984..2018
                     /locus_tag="ACUBV3_000650"
     CDS             984..2018
                     /locus_tag="ACUBV3_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HHU3847177.1"
                     /translation="MDNKQHNTSMISLLQYLFSILVILVHSGRLFSQDVIHFTFKSFL
                     GRMAVPYFLICTAFFLRGRIQQGLCNHSYFRKLIKKYSMWTIIYLPYGYFFFESLNIA
                     KIYLLPGFIVAFLYLGMSHTLWYIPAVILGWVIIQGLLKYVGTRGTFITVVVLYCIGA
                     VETYSVFIQSTKFYPLMSTYMSIFQTTRNGLFYTPVYLLAGYLLYDYFNTDLFTKSRG
                     LKYILFLLLLALENVLIYFNQGLDKNFFLLAPLCAVFLFNWSIRTSLFKEYRLSPLKQ
                     LSVYYFFLPPLFIGIVSYCLKSTSLVAHHQGKVIFVVTLALTHACALLIIKVVNKIPY
                     QKIIPLSLSK"
     gene            2265..2690
                     /locus_tag="ACUBV3_000651"
     CDS             2265..2690
                     /locus_tag="ACUBV3_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HHU3847178.1"
                     /translation="MKRKFLIGSHGKLASGLQSSIDILTGKGQEIQTIDAYIDDSDYT
                     KSIVEFIDEIAPDEQGLIFTDLLGGSVNQKMATAVMNSGKNNIFLITNSNLATLLSLL
                     FLKPEEELTKEEIVTVINESQVQLVDLSFKAGSEDDFFD"
     gene            2707..3198
                     /locus_tag="ACUBV3_000652"
     CDS             2707..3198
                     /locus_tag="ACUBV3_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HHU3847179.1"
                     /translation="MITQIRVDDRLIHGQVAVVWTKELNAPLLVVANDEAAKNEITQM
                     TLKMAVPNGMKLLIRSVEESIALFKDPRATDKRIFVIVNSVKDACTIAKNITDLEAVN
                     VANVGRFDKSDPATKVKLTSSLLLNTEELEAAKELASLPDLDVFNQVLPSNTKVNLSQ
                     LVK"
     gene            3214..4023
                     /locus_tag="ACUBV3_000653"
     CDS             3214..4023
                     /locus_tag="ACUBV3_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HHU3847180.1"
                     /translation="MIMPATMAALAVLICFGGNYLTGQSMMERPLVVGLVTGMLLGDI
                     KVGILMGASLEALFLGNVNIGGVIAAEPVTATAMATTFTIISNIDQKAAMTLAVPIGM
                     LAAFVVMFLKNVFMNIFAPMVDKAAAANHQGKLVMLHYGTWIIYYLIIASISFIGILV
                     GSGPVNSFVHHIPQNLMNGLSAAGGLLPAVGFAMLMKLLWTNKLAVFYLLGFVLTAYL
                     KLPAVAVAALGAVICVISSQRDIELDAITRGAISKQTTFDSKESEEEDFFA"
     gene            4020..4847
                     /locus_tag="ACUBV3_000654"
     CDS             4020..4847
                     /locus_tag="ACUBV3_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HHU3847181.1"
                     /translation="MKSQDNLTKEDRKMLRSVFWRSWTMNASRTGATQYHAVGVIYTL
                     LPVINRFYKTDKDKAEALVRHTTWFNATMHINNFIMGLVASMEKKNSEDPDFDASAIT
                     AVKASLMGPISGVGDSFFWGILRVIAAGIGISLASTGSAMGAVVFLLLYNIPAFLIHY
                     YSLYGGYSVGAGFIKKLYESGGIKIVTKTSSMLGLMMVGSMTASNVKFKTILTVAAKG
                     AKEAASIQSYLDQLFVGVVPLLVTILAFWLLRKKVNINWIMFGIMVLGIVLGLLGIC"
     gene            4985..6634
                     /locus_tag="ACUBV3_000655"
     CDS             4985..6634
                     /locus_tag="ACUBV3_000655"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HHU3847182.1"
                     /translation="MRGYRMEERFKKRLQDDISKHFSRQSLILSLLLIALFVLFSLAP
                     QQIGLYKDVNSVSYSYKQLIQKHDTLLDDLGKNSLKPFVSGHLGSADLSKQYYHLRNH
                     LQSQTELLVFSPNQELLFASNSHLGNFFSKSIYISEVLDKAKINQRLLKIIVDSEGGH
                     YLALIKPIIVNKKVSGYAFLLMNGKDFLLPTKAINSDLIIADQLNNSFTFTNRDFISS
                     SLDKVDSQFLTRYFSFHDHRAFVVRKVALQDNILLYMYRPLIPVTLVVLFSLVSSVII
                     FVILRQKSRVLADRIAVKNSSAINQMVLDMDAISRQEKSSIELDSQDEFQYLSVQINQ
                     MVSQLKDLHEKTLDLETQKLLFEKRMLEAQFNPHFLYNTLETILITSHYDSQLTERIV
                     IQLTKLLRYSLSGSTEAAVLKDDLAIIESYLLINQVRFEELTYTISVSPELEHMRVPK
                     LFLLPLIENAIKYGLKERHDVAINIDIWQDSDGIWFTVSNNGSGISLARQQAIRTMLR
                     STHSHHGLINSYRRLQYQFSTVLLEFTKTDDAFRVSYIVKE"
     gene            6638..7411
                     /locus_tag="ACUBV3_000656"
     CDS             6638..7411
                     /locus_tag="ACUBV3_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HHU3847183.1"
                     /translation="MYRLLIVEDEHLIRKWLRYAIDYQSLNILVVGEAKDGKEGAQLI
                     QEEQPDIVLSDINMPIMTAFDMFEATKGQSYAKIILSGYADFPNAQSAIHYGVLEFLT
                     KPLEKQALIDCLKTIMARIEEHKEKHLQEHTELYLPLPQANDQVPEVIKDMLAWIHSH
                     FHGKIVISQLAHDLGYSESYLYTVTKKHLHITLSDYINQYRINQAIQLMFREPDLMVY
                     QIAEAVGIYDYRYFDRVFKKYLGQTVKAFKEEHIFKQMD"
     gene            7423..8460
                     /locus_tag="ACUBV3_000657"
     CDS             7423..8460
                     /locus_tag="ACUBV3_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HHU3847184.1"
                     /translation="MKEKQSKRLIYILLVVSIIFISVFTYSISQPSKLLPPKELVILS
                     PNSQAILTGTIPAFEEKYGIKVKLIQGGTGQLIDRLSKEGKQLKADIFFGGNYTQFES
                     HKALFESYVSKNVHTVIPDYIHPSDTATPYTINGSVLIVNNELAKGLTIKSYEDLLQP
                     SLKGKIAFADPNTSSSAFSQLTNILLAKGGYTNPKAWNYVKKLQHNINAIKSSSSSEV
                     YQSVAEGKMIVGLTYEDPSVNLQKSGANVSIVYPTEGTVFVPSSVAIIKNAPSMKEAK
                     LFINFMLSLDVQNAFGQSTSNRPIRKDAQTSNGMKALKDIATLKEDYRYVTKHKGQIL
                     KTYNRIRRNAD"
     gene            complement(8682..9179)
                     /locus_tag="ACUBV3_000658"
     CDS             complement(8682..9179)
                     /locus_tag="ACUBV3_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HHU3847185.1"
                     /translation="MVATLIIIRGNSASGKSTIAKQLQAELGENTLLLSQDYLRREML
                     GTKDGENTTTIPLLINLLNYGYHNCSYIILEGILRSDWYTPVWKHILKHNPNNTYAYY
                     YDLSFQETVKRHSTRLKSLEFGEDSLARWWLEKDFLKEIPEKILTKAMSLEDVKKLIM
                     TDVLP"
     gene            complement(9179..10216)
                     /locus_tag="ACUBV3_000659"
     CDS             complement(9179..10216)
                     /locus_tag="ACUBV3_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HHU3847186.1"
                     /translation="METTLNYIKTLTSIPSPTGFTQTIMTYIIKELEAFGYSPIRTNK
                     GGVMVSLKGKNDTKHRMITAHLDTLGAMVRAIKPDGRLKIDLIGGYTYNAIEGENCTI
                     HLSKNGKEISGTALIHQTSVHVYKDAGTAERNQTNMEIRLDEKVTTADETRALGIQVG
                     DFISFDPRTIITDSGFIKSRYLDDKVSAGILMELLSVYKKEDIQLPYTTHFYFSAFEE
                     LGHGANSSIPNETVEYLAVDMGAMGDDQETDEYTVSICVKDASGPYHYELRQHLVSLA
                     ENNNIPYKLDIYPYYGSDASAAMRAGAEVKHALLGAGIESSHSYERTHIDSIQATELL
                     VDAYLKSNMVD"
     gene            complement(10227..10682)
                     /gene="nrdI"
                     /locus_tag="ACUBV3_000660"
     CDS             complement(10227..10682)
                     /gene="nrdI"
                     /locus_tag="ACUBV3_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HHU3847187.1"
                     /translation="MPKLTLVYISLSGNTQSFVKRLSEQLSTWHNCQVSTINIKEQNH
                     QTFPVDQPFVALLPTYLEGGNGIDNGDQEILTIPLGDFIAAHNNVKNCIGIVGSGNRN
                     FNNQYCLTAKQYSERFGFPMLGDFELRGTSSDVERIANIIVETLSNFKA"
     gene            complement(10839..13241)
                     /locus_tag="ACUBV3_000661"
     CDS             complement(10839..13241)
                     /locus_tag="ACUBV3_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase/3'-nucleotidase"
                     /protein_id="HHU3847188.1"
                     /translation="MSKHYFSKSVFALTVLTATATSGLAVQAEDIVTTPSSTSTKVES
                     TTPTSTIAEEKSNVTSTPTAITDASTATSTNTTTNNQNPQPVATEATTSDLKPIEGEK
                     VDLRIMSTTDLHTNLVNYDYYQDKESQKIGLAKTAVLIEEAKKENPNTVLVDNGDVIQ
                     GTPLGTYKAIVKPVAENEEHPMYQAMNALGYDASTLGNHEFNYGLDYLKKIIATANLP
                     ILNANVLDFKTHQPVFKTYDIITKTFKDSTGRAVALNIGITGIVPPQILNWDKANLEG
                     KVIVKDSVKAIEEIVPTMRAKGADVILVLSHSGIGDDRYEEGEENVGYQIASIKGVDA
                     VVTGHSHAEFPSGNGTGFYEKYTGVDGINGKINGTPVTMAGKYGDHLGIIDLGLSYTN
                     GKWQVSESSAKIRKIDMNSTTADERIIALAKEAHDGTINYVRQQVGTTTAPITSYFAL
                     VKDDPSVQIVNNAQRWYVANELKGTPEANLPLLSAAAPFKAGTRGDATAYTDIPAGPV
                     AIKNVADLYLYDNVTALLKVTGADLREWLEMSAGQFNQIDPNNKAPQNIINTEYRTYN
                     FDVIDGLTYKFDITQPNKYNKDGKVVNSQASRVRDLMYNGKPVADKQEFMIVTNNYRA
                     SGTFPGAKNATMNRLLNLENRQTIINYIISEKTINPTADNNWGFTESIKDLDLRFQTA
                     DKAKNLVTNSKDIQYIASSTKDEGFGDYRFVYTEQEKVDLTEPSHQEKLSQIEKQKIH
                     HMVATAYTKAKASPNITGTVYTQQKEKTLPKTGQEKQSYLAFFGVAILGLVGSLLKKK
                     QI"
     gene            13501..15717
                     /locus_tag="ACUBV3_000662"
     CDS             13501..15717
                     /locus_tag="ACUBV3_000662"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019316080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HHU3847189.1"
                     /translation="MVKEINLTGEEVVAITSQYMSETDVAFVKFALNYATAAHYYQAR
                     KSGEPYIIHPIQVAGILADLHLDAVTVACGFLHDVVEDTEITLDEIETDFGKDVRDII
                     DGVTKLGKVEYKSHEEQLAENHRKMLMAMSKDIRVILVKLADRLHNMRTLKHLRKDKQ
                     ERISRETMEIYAPLAHRLGISRIKWELEDLSFRYLNETEFYKISHMMSEKRREREELV
                     DIIVDKIRSYTEEQGLYGDIYGRPKHIYSIYRKMRDKKKRFDQIYDLIAIRCIMETAS
                     DVYAMVGYIHELWRPMPGRFKDYIAAPKANGYQSIHTTVYGPKGPIEIQIRTKEMHQV
                     AEFGVAAHWAYKKGITSKVNQAEQSVGMGWIQELVELQDESKDAKDFVDSVKEDIFTE
                     RIYVFTPNGAVQELPRESGPIDFAYAIHTQVGEKATGAKVNGRMVPLTAKLKTGDVVE
                     IITNPNSFGPSRDWIKIVKTNKARNKIRQFFKNQDKETSINKGRELLVDYFQEQGYVP
                     NKYLDKKHIEEILPRVSVKSEEALYAAVGFGDLSPISIFNKLTEKERREEERAKAKAE
                     ADELINGGEIKTDKRDVLKVKSENGVIIQGASGLLMRIAKCCNPVPGDLIEGYITKGR
                     GVAIHRSDCQNLKSQENYEQRLIDVEWDDDGSKKEYMAEIDIYGLNRSGLLNDVLQTL
                     SNATKLVSTVNAQPTKDMKFANIHVSFGISNLAQLTTVVDKIKIIPDVYSVKRTNG"
     gene            15727..16170
                     /gene="dtd"
                     /locus_tag="ACUBV3_000663"
     CDS             15727..16170
                     /gene="dtd"
                     /locus_tag="ACUBV3_000663"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - peptidyl-tRNA hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HHU3847190.1"
                     /translation="MKIIIQRVNQASVSIEDDVVGSIEKGLVLLVGIAPEDTTEDIAY
                     AVRKITSMRIFSDDEGKMNLSIQDIKGSVLSISQFTLFADTKKGNRPAFTGAADPVKA
                     NQFYDIFNQELANHVSVETGQFGADMQVSLINDGPVTIVLDTKNK"
     gene            16425..17348
                     /locus_tag="ACUBV3_000664"
     CDS             16425..17348
                     /locus_tag="ACUBV3_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter solute-binding protein,
                     Zn/Mn family"
                     /protein_id="HHU3847191.1"
                     /translation="MKRIRKSLIFVLGVVTLICLCACTKQSQQKNGLSVVTSFYPVYS
                     ITKAVSGDLNDIKMIRSQSGIHGFEPSSSDVAAIYDADLFLYHSHTLEAWARRLEPSL
                     HHSKVSVIEASKGMTLDKVHGLEDVEAEKGVDESTLYDPHTWNDPVKVSEEAQLIATQ
                     LAKKDPKNAKVYQKNADQFSDKAMAIAEKYKPKFKAAKSKYFVTSHTAFSYLAKRYGL
                     TQLGIAGVSTEQEPSAKKLAEIQEFVKTYKVKTIFVEEGVSPKLAQAVASATRVKIAS
                     LSPLEAVPKNNKDYLENLETNLKVLVKSLNQ"
     gene            17375..19956
                     /locus_tag="ACUBV3_000665"
                     /pseudo
     CDS             17375..19956
                     /locus_tag="ACUBV3_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080375.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
     gene            complement(20238..21014)
                     /locus_tag="ACUBV3_000666"
     CDS             complement(20238..21014)
                     /locus_tag="ACUBV3_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HHU3847192.1"
                     /translation="MLKRERLQKIIEKVNINGIVTVNEIMEELDVSDMTVRRDLDELD
                     KAGLLIRIHGGAQKVNASPTPQNYEKSNTEKYDIQTNEKLEIAQFAKQFINDGETIFI
                     GPGTTLEKLATQLLDFKIRVVTNSLPVFNILNQSSTLDLILVGGEYREITGAFVGSVT
                     INSIKSLNFSKAFVSSNGVFEKSIATYDEGEGEIQRIALNNSFEKFLLVDSQKFGKYD
                     FYTFYQLDDIDFVLTDHNIDNVVKEQYSSFTKILTNNNYM"
BASE COUNT         6719 a         3440 c         4021 g         6849 t
ORIGIN      
        1 tgggttctgc gctaccacta aaacaactaa agctaggtaa ctttgctcct tggaatcaac
       61 ttttcgaagt aagtaatagt acttatattt taattgtctt atttactcta ttcttatcag
      121 ctcctctttt ttcaggagat agggctaaga agattaatcc tatactactg acgactcggt
      181 atggacgtgg tcagttgacc tgggctaagt tagctagtac aatgaccatc ggtatttcca
      241 catttatcct agcatatgcc ctcattctga cggtatttgc agattatttt ggttttagtg
      301 gttgggagac aagcgttcaa ctgaatttat actggacatc acctttggct aatattatgg
      361 gatttcctgt acctatgaca atatcaactg cttttgttca tattattgtc ttacaatttg
      421 ttggactcct ttttatagcc agtcttaatt tctttatatc aagtatcaca aattcgccat
      481 taacatcttt tgctgtttca acgcttgcct tttttgctcc tagcttttta atggctatgt
      541 ttaataaagg tgtgataaac aagttattaa ctattttctc agtatcaacg acagacactt
      601 cagcttcact tttaaaatta tcacgtaatg gtagtcatgg ctttttcttt gatagttttt
      661 ctgctaatga aatctgtctg attagtctac gtatgttaat tgcagtgtta ttgatagcct
      721 atacctatcg tttaatgcga cgaagaagtt tatagttccc actggttatc tgaacaagaa
      781 attattctaa gctgattttc ttttgcgaaa tcagcttttt tatttcctaa tatggttgat
      841 aaggtattaa caataattat cagataccat ttaaggcgtt tcaatgactt tttgggataa
      901 ttgagatatt cttctgaaga gattggtatc atagtaatga taacttatag aaagtaatgt
      961 taaggatgaa gagaagtcag tgcgtggata ataagcagca taatacatca atgattagtt
     1021 tgctacaata ccttttttcc atactggtca ttttagttca tagtgggagg ttatttagtc
     1081 aggatgtcat tcattttacc tttaaaagtt tcctaggtcg gatggctgtt ccttattttt
     1141 tgatttgtac tgctttcttt ttgagaggtc gtatacagca aggtttgtgt aatcactcct
     1201 actttcgtaa actgattaaa aagtatagca tgtggactat catttatttg ccttatggtt
     1261 atttcttttt tgaatctcta aatatagcga agatctattt gttgccgggg tttattgtgg
     1321 catttttgta tttaggtatg tcacataccc tttggtacat ccctgctgtt attctaggtt
     1381 gggtcattat tcaaggatta ttgaagtatg taggaactag ggggacattt atcacagttg
     1441 ttgtactcta ttgtattgga gcagtagaga cctattctgt ctttatacag tcaacaaaat
     1501 tttatcccct gatgtccact tatatgtcaa tttttcagac aacacgaaac ggattatttt
     1561 acactccagt ttatcttcta gcaggatatt tactctatga ttattttaat actgacctat
     1621 ttaccaaatc gcgtggtctt aagtatattc tcttcctttt acttctagct ttagagaatg
     1681 ttcttattta ttttaatcaa gggttggata aaaatttctt cttattagct cctttatgtg
     1741 cagtattctt atttaactgg agtatacgta catcactgtt taaggaatat cgtttaagtc
     1801 ctttaaaaca gttgagtgtc tattatttct tccttccccc tctttttatt ggcatagtgt
     1861 cttactgttt aaaatcaact tccttagttg cacaccatca aggaaaagtg atttttgtgg
     1921 tgaccttggc attaacacat gcttgtgctc tactcataat aaaggttgtt aataagatac
     1981 cctatcaaaa aattattcca ttatctcttt cgaaatagtg atgcatataa gagaaagttt
     2041 aaagttatag tgatgtaaag gttgtgtaaa ctattaggat acatgacctt tttattgttc
     2101 tgtttttaaa tattaattat gtagaatagt ccacatatag agtagaatag tccattttta
     2161 aaccgctttc atttgttaga atgatagtaa aggttatctt attcaaagta ttgataaaac
     2221 tttcagaact aatttagggt aatgtaataa ataggaggag aaagatgaag cggaaattcc
     2281 taatcggcag tcacggaaaa cttgcgagtg ggttgcagag ttctattgat attctgacag
     2341 gcaagggaca agagattcag acaatagatg cttacattga tgatagtgac tatactaagt
     2401 ctattgtaga atttattgat gagattgcac cagatgaaca ggggcttatt tttacagatt
     2461 tgttaggtgg cagcgttaat cagaaaatgg cgactgctgt aatgaatagt gggaaaaata
     2521 atattttttt aataacgaac agcaatttag cgaccttgtt gtcattacta tttttgaaac
     2581 ctgaagagga attaactaaa gaagaaattg taaccgttat caatgaatct caagtgcagc
     2641 tggtagatct tagctttaaa gcgggttcag aagatgactt tttcgattaa taattaggag
     2701 gaaaaaatga ttactcaaat tcgtgttgac gaccgtttga ttcacgggca agtggcagtt
     2761 gtatggacta aagaattaaa tgcaccactt ttagtggtgg ctaatgatga agctgcaaaa
     2821 aatgaaatca cacagatgac tcttaaaatg gctgtgccta atggcatgaa attattaatc
     2881 cgttcagttg aagaatcaat agctctattt aaagatccac gtgcaactga caagcgcatt
     2941 tttgtgattg tcaattctgt taaagatgcc tgtacaattg ctaaaaatat aaccgattta
     3001 gaagcagtaa acgtcgctaa tgtaggacgc tttgataagt ctgatccagc tacaaaagtc
     3061 aaactaacat caagtctctt attaaataca gaagagttgg aagccgcaaa agaattagcc
     3121 agcttaccag atttagacgt ctttaaccaa gtattgcctt caaatactaa ggttaacttg
     3181 agtcagttag tcaaataaag tgaggtatga catatgataa tgccagcgac gatggcagct
     3241 ttagctgtat tgatttgttt tgggggaaat tacttaacag gtcaaagtat gatggagaga
     3301 ccacttgtag ttggtcttgt aacaggaatg ctactaggtg atattaaggt tggtatccta
     3361 atgggagcct ccctagaagc gctatttttg ggaaacgtta atattggggg tgttattgcc
     3421 gcagagccag taacagcaac agcgatggca acaactttta ccattatttc aaacattgat
     3481 caaaaagcag caatgactct agctgttccg attggaatgt tggcagcatt tgttgttatg
     3541 tttttgaaaa acgtcttcat gaatatcttt gcaccgatgg tggataaagc tgcagccgca
     3601 aaccatcaag gtaaacttgt gatgcttcac tatggtacat ggattattta ctatctcatt
     3661 atcgcttcta tttctttcat tggtatttta gtaggtagtg ggcctgttaa tagttttgtc
     3721 catcatattc ctcaaaacct gatgaatggt ttgagtgcgg cgggaggcct ccttccagct
     3781 gttggttttg ccatgctgat gaaattatta tggacaaata aattagctgt tttttacttg
     3841 cttggttttg ttttaacagc ttatttaaaa ttaccagcgg ttgcagttgc tgccctagga
     3901 gcagttattt gcgtcattag ttcacaacgt gatatagaat tagacgcaat tactagaggt
     3961 gcgatcagta aacagacaac ctttgactct aaagaatcag aagaggagga cttcttcgca
     4021 tgaaatcaca ggataatttg actaaagaag accgtaaaat gcttcgctca gtcttctggc
     4081 gctcatggac gatgaatgcc agtcgtactg gggcaacaca ataccacgca gtaggagtta
     4141 tttatacttt actaccagtc atcaatcgtt tttataagac tgataaggat aaagcagagg
     4201 cactggtccg tcatacaaca tggtttaatg ccaccatgca cattaataat tttattatgg
     4261 gtcttgttgc atctatggaa aagaaaaaca gtgaagatcc tgattttgac gctagtgcta
     4321 tcaccgcagt taaggcttct ttgatgggac ctatatctgg tgttggtgat tcattctttt
     4381 ggggaattct aagggttatt gcggcaggta tcggtatctc cttagcaagt acaggctcag
     4441 ccatgggagc tgttgtcttt ctactcttgt ataacattcc agcttttctg attcattatt
     4501 atagtctcta tggaggttac tctgtaggtg cagggtttat caaaaaattg tatgaatcag
     4561 gtggtattaa aattgttact aagacttcaa gtatgcttgg tttaatgatg gtaggctcaa
     4621 tgactgcttc aaatgttaaa ttcaagacaa tcttaacggt agcagctaaa ggagctaagg
     4681 aagcagcttc catacaaagt taccttgacc aattatttgt tggcgtagtg ccactgttgg
     4741 taacgatatt agccttctgg cttctacgta aaaaagtcaa tatcaactgg atcatgtttg
     4801 gtattatggt gttaggtatt gtacttggtc tcttaggtat ttgctaataa tctcctagca
     4861 ttaaaggtaa gtggaacaag acgatacttg tttagcttat ctttttttct tttgaaagtt
     4921 attgtatgaa aggagttgac tatctttatt ggatagctat ttgctataat aaaaggagat
     4981 ggaaatgaga ggatatagaa tggaagaacg ctttaagaaa cgactacaag atgatatctc
     5041 taaacatttt tcccgccagt ctttaatttt atccttgtta ttgattgcac ttttcgtcct
     5101 tttttcttta gcgcctcaac agataggtct ttataaagat gttaattcag tttcctatag
     5161 ttataagcaa ttgattcaaa aacatgatac cttattagat gacttaggta aaaatagttt
     5221 gaaaccattt gtctcaggac acttaggaag tgctgatttg agtaagcaat attatcattt
     5281 aaggaatcat ttacaaagtc aaacagaact tttagtattc tcacctaatc aagaactttt
     5341 gtttgctagt aattcacatt taggtaattt ttttagtaag tctatttaca ttagtgaagt
     5401 tttagataag gctaaaataa atcaaaggct actaaaaatt attgtggata gtgagggagg
     5461 gcattacctt gcactcatta agccaattat agttaataaa aaagtttcag gttatgcctt
     5521 tttattgatg aatgggaaag actttttact tccaacgaag gctattaact cagacttgat
     5581 tattgccgat cagctaaata atagctttac ttttactaat cgtgatttta tctcctctag
     5641 tttagataag gtagatagtc aattcttaac tagatatttt agttttcacg atcatcgagc
     5701 ttttgttgtt aggaaagtag ctctgcaaga caatatccta ctttacatgt atcgtcccct
     5761 gataccagta actctggtcg tccttttttc tctagtatca tcagttatta tttttgtgat
     5821 tttacgacaa aaatctagag ttctagccga tcgcatcgca gtaaaaaatt ctagcgccat
     5881 aaatcaaatg gtgctagata tggatgccat ttcccgccaa gaaaaatcta gtattgagct
     5941 tgatagtcaa gatgaatttc aatatctatc tgtgcaaatc aatcagatgg tttcacagtt
     6001 aaaagattta catgagaaaa ctcttgattt ggaaacacaa aagttgttgt ttgaaaagag
     6061 gatgttagaa gctcagttta atcctcactt cctttataat acccttgaga caattttaat
     6121 aaccagtcac tatgattcac aattaacaga aagaatcgtt atacaactaa caaaattatt
     6181 gcgctatagc cttagtggta gcacagaagc agctgtgctt aaggatgatt tagcaatcat
     6241 tgaatcctac cttttgatta atcaagtgag atttgaagaa ttaacttaca ctatttcagt
     6301 atctcctgaa cttgaacaca tgcgtgtacc aaaacttttc ttgttaccgc taattgagaa
     6361 tgctattaaa tatggcttaa aagaacgtca tgatgtagca ataaacatag atatttggca
     6421 agatagtgat ggaatttggt ttactgtttc aaataatgga tcaggtatta gtttggctag
     6481 gcaacaagcc atacgtacaa tgttaagatc aactcactcg catcatgggc ttattaattc
     6541 ttatagacga ttacaatatc aattttcaac agtattgtta gagtttacga agacagatga
     6601 tgcttttcga gttagctata tagtaaagga gtgagttatg tatagattac ttatcgttga
     6661 ggatgagcac cttattcgta agtggttacg ctatgctatt gattatcaat cattgaatat
     6721 tttagtggtt ggtgaagcaa aagatggtaa agaaggagct caattaattc aggaagagca
     6781 gccagacatt gttttaagtg atattaatat gccaattatg acagcattcg atatgtttga
     6841 ggcaaccaaa ggacaatctt atgccaaaat tattttatct ggctatgctg attttccaaa
     6901 tgcgcaatca gctattcact atggcgttct tgaattttta acaaaaccac tagaaaagca
     6961 agctttgatt gattgtttaa aaacaattat ggctagaatt gaggaacata aggagaagca
     7021 cctacaagag catacagaat tgtatttacc tctaccacaa gctaatgacc aagttcctga
     7081 agttattaaa gatatgctag catggattca tagccatttt catgggaaaa ttgtgattag
     7141 ccagttagct cacgatttag gttacagtga aagttatctt tacacagtca ctaaaaagca
     7201 ccttcatata actttgagtg attatataaa tcaatatcgt attaatcaag ccattcagtt
     7261 gatgtttaga gaaccagacc taatggttta ccaaattgca gaagctgttg gtatttatga
     7321 ttatcgttat tttgataggg tttttaaaaa gtacttgggt cagacagtaa aagcgtttaa
     7381 ggaagaacat atttttaaac aaatggatta ggtaggtgaa agatgaaaga aaaacagtcg
     7441 aaaaggctta tttatatact actggttgtt tccattattt ttataagtgt ttttacatac
     7501 agtattagcc agccttctaa actacttcca ccaaaagaat tagttattct aagtccaaat
     7561 agtcaagcca ttttaacagg aacgattcca gcttttgagg aaaaatacgg tataaaagtt
     7621 aagcttattc aaggtgggac agggcaacta atagatagat taagtaagga gggtaagcag
     7681 ttgaaggcgg atattttctt tggaggaaat tatacgcaat ttgaaagtca taaggcattg
     7741 tttgagtctt acgtatcaaa gaatgttcat actgttattc cagactatat ccatccaagt
     7801 gatacggcga caccttatac tataaatggg agtgtcttga ttgtaaataa cgaattagct
     7861 aagggactta ccatcaagag ttatgaagat ttattacagc cttccttaaa aggtaaaatt
     7921 gcctttgcag atccgaatac ttcctctagt gctttctcac aactcactaa tatactcttg
     7981 gccaagggtg gttacaccaa tccaaaagcg tggaactatg ttaaaaagct acaacataat
     8041 attaatgcta tcaaatcttc tagctcttca gaagtttatc aatcagttgc agaaggaaaa
     8101 atgattgtgg ggctgactta cgaagaccct agtgtcaatt tgcaaaaaag tggtgccaat
     8161 gtttctattg tatatccgac agaagggaca gtttttgtcc catcttcggt tgcaattata
     8221 aagaatgctc cttctatgaa agaagcaaag ttatttatta attttatgct ttctttagat
     8281 gttcaaaatg cctttgggca gtcaacgagt aaccgaccta ttcgtaaaga tgcccaaacg
     8341 agtaatggca tgaaagcttt aaaggatatt gctactctta aagaagatta tcgctatgtc
     8401 actaagcata agggccaaat ccttaaaacc tataatcgta ttcgtagaaa tgctgattaa
     8461 atgagaatat agaaaaactc cttggaatca ttgattccga ggagtttaat cgtcgtcttc
     8521 gtcgctttcg aaatcttttt tccaataacg ataaagacta atacatgcca gaaatccggc
     8581 tataatggga acgataataa ataagaggtc aatcactgta tccatgggca atatactcca
     8641 atgataagaa gtcattattg tttatagtgc gtacaataat gttaaggtaa aacgtcggtc
     8701 attataagtt ttttgacatc ctctaaactc atagcttttg ttaatatttt ttcaggtatc
     8761 tcttttaaga aatctttctc aagccaccat cttgcaagag aatcttctcc aaattctaaa
     8821 gatttaagtc gagttgaatg tcttttaaca gtttcttgaa agcttaaatc atagtaataa
     8881 gcataagtat tgttggggtt atgttttaaa atatgtttcc aaacaggcgt ataccaatca
     8941 gaacgaagta tgccttcaag gatgatgtag gaacagttat ggtaaccata atttagtaaa
     9001 ttgatcaaaa ggggaatggt agtagtattt tcaccatcct tagttcctaa catctctcta
     9061 cgaaggtaat cctgagataa gagaagggta ttttccccta attcggcttg aagttgctta
     9121 gctattgtag actttccgct agctgaattt cccctgataa taatgagcgt ggctaccatt
     9181 agtccaccat attgcttttg agataggcat ccactaagag ttcagttgct tgaatagaat
     9241 cgatatgggt acgttcataa gaatgactag attcaatacc tgcaccaagt agcgcgtgtt
     9301 taacttccgc accagcacgc atggcagcgg aggcgtcact accataataa ggataaatat
     9361 caagtttata aggaatatta ttgttttcag ctagagaaac aagatgttga cgtaattcat
     9421 aatgataagg accagaagca tctttaacac agatagagac agtatattcg tcagtttctt
     9481 gatcgtctcc catagctccc atatcaactg ctagatattc tacagtttca tttgggatgc
     9541 ttgaatttgc tccatgtcct agctcttcaa aggcactaaa gtagaaatga gtagtataag
     9601 gaagttgaat gtcttctttc ttgtaaacag aaagaagttc cattaggata ccagcggata
     9661 ccttgtcatc taggtaacgt gatttaataa agccgctgtc tgttatgatt gtacgcggat
     9721 caaatgaaat gaaatcaccg acctggatgc ctaaagcacg tgtttcgtca gcagttgtta
     9781 ctttctcatc taaacgaatt tccatatttg tttgattacg ttcagcagtt ccagcgtctt
     9841 tgtaaacatg gacactagtt tgatgaataa gagcagttcc agaaatttct ttaccatttt
     9901 ttgagaggtg tatagtacag ttttctcctt caatggcatt gtatgtatat ccaccaataa
     9961 ggtcaatttt taaccgacca tctggtttga tggccctaac catagcacca agtgtatcaa
    10021 ggtgagcagt tatcatgcga tgtttagtat cattttttcc ttttagagaa accatgacgc
    10081 ctcccttgtt tgtgcgaatt ggtgagtagc caaacgcttc caattctttg atgatatagg
    10141 tcatgattgt ttgggtaaac cctgttggtg aagggattga tgtcagggtt ttaatgtagt
    10201 ttaatgttgt ttccatacta tttcctttag gctttaaagt tcgataatgt ttcaacgata
    10261 atatttgcga tacgttcaac gtcgctagaa gttcctcgca attcaaagtc tcctaacata
    10321 ggaaatccaa accgttcaga gtattgtttt gcagttaaac agtactgatt gttaaaattt
    10381 ctatttccag agccaacaat tccgatacaa tttttgacat tattgtgtgc agctataaaa
    10441 tcaccaagtg gaatggtaag aatttcttga tcgccattgt caatgccatt tcctccctca
    10501 aggtaagtgg gtaatagtgc tacgaaaggt tggtcaacag gaaaggtttg atgattttgt
    10561 tccttgatat taatagtcga aacctgacag ttatgccaag ttgacagttg ttcactaaga
    10621 cgtttgacaa agctttgagt attgccgcta agactgatat agactagggt taatttaggc
    10681 atttagtact ccttaaatct aaatttcttg ttatcagttt agcaaaatca tgtcagataa
    10741 acaagctaga gtggtatctc aatatctgac tatttagaga gaaaatctat tgttgagact
    10801 aaaaactgag gttgattggc ctcagttttt agtgatgact atatttgttt ctttttaagt
    10861 aaacttccca cgagtccaag tattgcgaca ccaaagaaag ccagataaga ttgtttttct
    10921 tgtccagttt taggtaatgt tttttctttc tgttgtgtat agacagtgcc tgtaatattt
    10981 ggagaagctt ttgcttttgt ataagcagtt gcaaccatgt ggtgaatttt ttgtttttct
    11041 atttggctta acttttcttg atgtgatggc tcagtaaggt ctactttttc ttgttcagta
    11101 tagacaaatc gataatcacc aaatccttca tctttggtag aactagcgat atactgaata
    11161 tccttgctat tagtaacaag atttttagct ttatcggctg tttggaatcg taaatcaagg
    11221 tctttaatag actctgtgaa gccccagtta ttatcagctg taggattgat ggttttctct
    11281 gaaataatat agttgataat agtttggcga ttttctaagt tcagcaaacg gttcattgtg
    11341 gcatttttag ccccagggaa ggtaccacta gcacggtagt tattggtcac aatcataaac
    11401 tcttgtttgt ctgcaacagg cttaccattg tacattaaat cgcgtactcg actagcttga
    11461 ctattaacaa cttttccatc tttattgtat ttattaggtt gtgtaatatc aaatttataa
    11521 gtcaagccgt caataacgtc aaaattatag gtacgatatt cggtattgat aatattttgt
    11581 ggtgctttat tgttagggtc aatttggtta aattgaccag cggacatttc taaccattca
    11641 cgtaagtctg ctccggtcac ttttaaaagt gcagtaacat tatcataaag gtaaagatca
    11701 gctacgtttt taattgcaac aggtccagct ggaatatcag tatatgcggt agcatcgccg
    11761 cgtgttccag ctttaaatgg tgctgcagca gatagaagtg gtaaatttgc ctcaggtgtt
    11821 cctttcaatt catttgcaac gtaccaacgt tgagcgttgt taacaatttg aactgaaggg
    11881 tcatctttaa ctagtgcaaa gtaacttgta attggcgcag ttgttgtacc tacttgttgg
    11941 cgaacatagt tgatagtgcc atcgtgtgct tccttagcca atgcaatgat acgctcgtca
    12001 gcagttgttg agttcatatc aattttacgg attttagcac tactttcgga gacttgccat
    12061 tttccattag tataactaag tcctaaatca ataataccaa gatgatcccc gtacttgcct
    12121 gccattgtaa caggtgttcc atttattttt ccattgatac catcaactcc agtgtatttt
    12181 tcatagaagc cagtaccgtt acctgatgga aattcagcgt gtgagtgtcc cgtaacaacg
    12241 gcatccactc ccttgatgct ggcaatttga tagccaacgt tttcttcacc ttcttcatat
    12301 cgatcatctc caatgccaga gtgtgataaa acgagaatga catctgctcc tttagcacgc
    12361 atagtaggaa cgatttcttc gattgcttta actgaatctt tgacaataac ctttccctct
    12421 aggttagcct tgtcccagtt taagatttga ggaggtacaa ttcctgtaat cccaatattt
    12481 agtgcaacag ctcttcctgt tgaatctttg aaggttttag taatgatgtc ataagtttta
    12541 aaaactggtt gatgtgtttt gaaatccaaa acatttgcgt tgaggattgg aaggttggct
    12601 gtagcaatga tttttttgag gtaatctaaa ccgtaattaa actcatgatt acctagtgtt
    12661 gatgcatcat aaccaagagc attcatggct tggtacatag gatgttcttc attttctgcg
    12721 acaggtttaa cgattgcttt ataagttcct aaaggtgtcc cttgaatgac atctccgtta
    12781 tcaactaaga cggtattagg attttctttt tttgcttctt caataaggac agcagttttt
    12841 gctaacccta ttttttgaga ttctttatct tgataataat catagttaac taaattggtg
    12901 tgtaggtctg tggttgacat gatgcgaagg tcaacttttt caccttcaat aggtttgaga
    12961 tcactagttg tggcttctgt ggcaacaggc tgtggatttt ggttattggt agtggtattt
    13021 gtgcttgtgg ctgttgaagc atcagttatt gcagttggag tagaagtaac gtttgacttt
    13081 tcctctgcaa ttgtgctagt gggggtagtt gattcaacct ttgtagaggt tgaacttggt
    13141 gttgtaacaa tgtcttctgc ttgaacagct aaaccacttg ttgctgtagc tgttaagaca
    13201 gttaaagcga atacgctttt tgaaaaataa tgttttgaca taaatctcct ccattaaaac
    13261 attacatgta tattatccta atctaaaaac tattatagct tattgaaaac gtttaatcaa
    13321 tctcatttac gaattatttt tagtgattac accaaaaaca atcgtttatt tataaaggat
    13381 aacttagtta gacacattta cttggtattt aattcttttt gagcctctaa cttcttttat
    13441 ttttaggtta aaaagtgata aaataggtac aatctatcac tatgattaaa gaggataact
    13501 atggtcaaag aaattaattt aacaggagaa gaggttgttg caatcacatc ccagtacatg
    13561 agtgagacgg atgttgcttt tgtaaaattt gccttaaatt acgcaaccgc agctcattat
    13621 tatcaagcaa gaaagtcggg cgagccctac attattcatc caattcaagt agcaggtatt
    13681 cttgcagatt tacaccttga tgccgttacg gtagcttgtg gttttttaca tgatgttgta
    13741 gaagatacgg aaattaccct tgatgagatt gaaactgatt ttggcaagga tgttcgcgat
    13801 attattgacg gcgtaacaaa attgggtaaa gtggagtaca aatctcatga agaacagtta
    13861 gcagaaaatc accgtaaaat gttaatggct atgtctaagg atatccgtgt tattttggtt
    13921 aagctcgctg accgcttaca taatatgaga acccttaaac acctcagaaa agataaacaa
    13981 gagcgtattt cacgtgagac tatggaaata tacgctcctt tagcgcatcg tttggggatt
    14041 agtcgtatca agtgggaatt agaagattta tcttttcgtt acttgaatga gacggaattt
    14101 tataagattt ctcatatgat gagtgaaaaa cgtcgtgaac gcgaagagtt ggttgatata
    14161 attgtcgata aaatcagatc ctatactgag gaacagggtt tatatggtga tatttatggt
    14221 agaccaaaac acatttattc tatttacaga aaaatgcgtg ataaaaagaa acgctttgat
    14281 cagatttatg atttaattgc gatacgctgt atcatggaaa ctgctagtga tgtttatgcc
    14341 atggtggggt atatccatga gttatggcgt cctatgccag gaaggtttaa agattatatt
    14401 gcggcaccaa aggccaatgg ttatcaatct attcacacga cggtttatgg acctaaaggg
    14461 ccaattgaaa ttcagattcg aactaaagaa atgcatcaag ttgccgagtt tggtgttgca
    14521 gcccattggg cctataaaaa aggaatcacc agtaaggtta atcaagcaga gcaatcggtt
    14581 ggtatgggat ggattcaaga attagttgag cttcaagatg aatcaaaaga cgccaaggat
    14641 tttgttgact ctgttaagga agatatcttt acagaacgca tctatgtttt taccccaaat
    14701 ggtgctgttc aggaattgcc aagggaatct ggaccaattg attttgctta tgctattcac
    14761 acgcaagttg gagaaaaagc tactggcgct aaagtaaatg gacgtatggt tccattgact
    14821 gctaagttaa aaacaggaga tgttgttgag attatcacaa accctaattc atttggacca
    14881 agtcgtgact ggattaagat tgttaagacc aataaagctc gcaataaaat tcgtcagttc
    14941 ttcaaaaacc aagataaaga aacttctatt aataaaggta gagaattact ggttgattat
    15001 ttccaagaac aaggttatgt gcctaacaaa tatttagata agaaacatat tgaagaaatc
    15061 cttccacgtg tcagtgttaa aagtgaggaa gctttgtatg cagccgtagg ttttggtgat
    15121 ttgagtccga ttagtatttt taataaactg acggaaaaag aacgccgtga ggaggaacgt
    15181 gctaaggcta aagcggaagc agacgaactt attaacggtg gtgaaatcaa aaccgataag
    15241 cgtgatgttc tcaaagttaa gagtgaaaat ggtgttatca ttcaaggagc ttcaggttta
    15301 ttaatgcgta ttgcaaaatg ttgtaatccc gttcctggag atctcattga aggctatata
    15361 actaaaggta gaggcgtggc tatccatcgt tcagattgtc aaaatttaaa gagtcaggag
    15421 aactacgaac aacgtttgat tgatgttgag tgggatgatg atggatctaa aaaagagtac
    15481 atggctgaaa tcgatattta tggtttgaat cgtagtggcc tccttaatga tgttcttcaa
    15541 accttatcaa atgcaacaaa gctagtatcg acagtaaatg cacaaccaac aaaagatatg
    15601 aaatttgcta atattcatgt tagctttgga atttcaaatt tagcacaatt gacaactgtg
    15661 gttgataaaa tcaaaattat cccagatgtt tattctgtta aacgtacgaa tggttaggag
    15721 agcaaaatga aaatcattat tcaacgtgtt aatcaagcaa gtgtttctat tgaagatgat
    15781 gtagtcggat ctattgagaa gggtttagta cttctagttg ggattgctcc agaagataca
    15841 acagaagata ttgcttatgc agttcgtaaa attactagca tgcgtatttt ttctgatgat
    15901 gaggggaaaa tgaacctttc aattcaagac attaaaggaa gcgtcttatc tatctcacaa
    15961 tttacactct ttgcagatac caaaaaagga aatcgtccag cttttactgg ggcagctgat
    16021 cctgttaaag cgaatcagtt ttatgatatc tttaatcaag aattagcaaa tcatgtttcg
    16081 gtagaaacag gtcaatttgg agctgatatg caagtcagtc tgattaatga tggaccagta
    16141 actatcgttt tagatactaa aaataaataa aaaaccaaag agttgggcag aatgcttggc
    16201 tctttttatt gtggttctaa ttatactcct taacccattc atgtttatga aaagatttac
    16261 tgtagcttgg aaaggcatga ataaaggttg ttgatatgaa attaagtgca cttaacaata
    16321 ttaaaaatag aaaaatatta gatttatggt ttttcgttga cactaaaata agaatactct
    16381 aaaatgttaa ctggttaagt attttttatt ttaaaggagg tttaatgaaa aggatacgga
    16441 aaagccttat ttttgttctc ggagtagtta ccctaatttg cttatgtgct tgtactaaac
    16501 aaagccagca aaaaaatggc ttgtcagtag tgactagctt ttatccagta tattccatta
    16561 caaaagcagt ttctggtgat ttgaatgata ttaaaatgat tcgatcacag tcaggtattc
    16621 atggttttga accctcatca agtgatgttg ctgccattta tgatgctgat ctatttcttt
    16681 atcattcgca cacactagaa gcttgggcga gacgtttgga acctagtttg catcactcta
    16741 aagtatctgt aattgaagct tcaaaaggta tgactttgga taaagttcat ggcttagaag
    16801 atgtagaggc agaaaaagga gtagatgagt caaccttgta tgaccctcac acttggaatg
    16861 accctgtaaa agtatctgag gaagcacaac tcatcgctac acaattagct aaaaaggatc
    16921 ctaaaaacgc taaggtttat caaaaaaatg ctgatcaatt tagtgacaag gcaatggcta
    16981 ttgcagagaa gtataagcca aaatttaaag ctgcaaagtc taaatacttt gtgacttcac
    17041 atacagcatt ctcatactta gctaagcgat acggattgac tcagttaggt attgcaggtg
    17101 tctcaaccga gcaagaacct agtgctaaaa aattagccga aattcaggag tttgtgaaaa
    17161 catataaggt taagactatt tttgttgaag aaggagtctc acctaaatta gctcaagcag
    17221 tagcttcagc tactcgagtt aaaattgcaa gtttaagtcc tttagaagca gttcccaaaa
    17281 acaataaaga ttacttagaa aatttggaaa ctaatcttaa ggtacttgtc aaatcgttaa
    17341 atcaataggt agttatataa agaaaggacg caggatgaat cgtaaaaaaa cagttattat
    17401 tagtgcttta tcagtagctc tatttggtac tggtgtaggt gcttaccaat taggtagcta
    17461 taatgcccaa aaatcagaca atagtgtttc ttatgtgaaa acagataaat ctgatagcaa
    17521 agcacaagca acagctgtaa ataaaacgcc cgaccaaatc agtaaagaag aaggcatatc
    17581 tgcagaacaa atcgttgtta aaattactga cgacggttat gtgacttctc atggagacca
    17641 ttaccattat tacaatggga aagtgccata tgatgccatc attagtgaag aattgattat
    17701 gaaagaccct agctatgtct ttaataaagc tgatgtcatt aatgaagtca aagatggtta
    17761 tattatcaaa gttaacggta agtattacct ctatcttaaa gaaggtagca aacgaacaaa
    17821 tgttcgtaca aaggaacaaa tccaaaagca acgcgaagaa tggtctaagg gtggttctaa
    17881 gggagaatca ggaaagcata gtagtgcaaa aactcaagct ttatcagcca gcgtacgtga
    17941 agcgaaagct agcggtcgct atactacaga tgatggttat gtttttagtc caacagatgt
    18001 cattgatgac atgggagatg cctttttagt accacatggc gatcatttcc attatatccc
    18061 gaaagctgat ttatctccat cagagttatc agccgcacag gcatattgga accgcaagac
    18121 tggccgatca ggtaatagct caaaaccatc aaattcaagt tcatacattc atgcaagtgc
    18181 accaagcggt aatgtatcaa caggaagaca tgctaatgcc cctatttcta ttccaagagt
    18241 cactcacgca aatcactggt caaaaccagc tggtaatcat gctacggcac caaaacacca
    18301 tgcacctaca accaaaccaa tcaacaaaga ttctgcttta gacaaaatgt tgaaacgttt
    18361 atatgctcaa ccgttatatg ctcgtcacgt agaaagtgat ggtttggttt atgacccagc
    18421 acaagttaat gctttcacag caatcggtgt ttctattccg cacggtaatc atttccactt
    18481 tattcactat aaggatatgt ctccattaga gttagaagca acaaggatgg tggcagagca
    18541 tagaggacat catattgatg cattagggaa aaaagattct acagagaaac caaagcatat
    18601 ttctcatgaa cctaataagg aacctcacac agaggaagaa caccatgcag taacaccgaa
    18661 agaccaacgt aaaggcaaac caaatagcca gattgtctac agtgctcaag aaattgaaga
    18721 ggcaaaaaag ctggtaaata cacaacatct gatggttaca tttttgatgc caaagatatt
    18781 aaaaaagata caggtacagg ttatgtcatt ccacatatga cacatgagca ttgggtacca
    18841 aagaaagatt tatcagagtc ggaattaaaa gcagctcaag aatttctttc aggaaaatct
    18901 gaagcaaatc aagacaaacc aaaaacaggt aaaacagctc aagaaatcta tgaggcaatt
    18961 gaaccaaaag caattgttaa atctgaagat ttattatttg gaattgcaca agcgacagac
    19021 tataagaatg gtacatttgt aattcctcat aaagatcatt accattatgt ggaattaaaa
    19081 tggtttgatg aagaaaaaga tcttttagct gattcagata agacatattc tttagaagac
    19141 tatttagcta cggctaaata ttacatgatg cacccagaaa aacgtcctaa agttgaagga
    19201 tggggtaaag atgctgaaat ttataaggaa aaggactcta ataaagcaga taaaccaagt
    19261 cctgcaccaa ctgataataa atcaacatca aattctagtg acaaaaactt aagtgctgca
    19321 gaagtattca aacaagcaaa accagaaaaa attgtaccgc ttgataaaat tgctgctcac
    19381 atggcatatg cagttggatt tgaagatgat caattgattg ttcctcatca tgatcattat
    19441 cataatgttc ctatggcatg gtttgacaag ggtggtttat ggaaagcacc agaaggctat
    19501 acattacaac aactcttctc aacaattaaa tactacatgg aacatcctaa tgaattacca
    19561 aaagaaaaag gttggggaca cgacagtgat cataacaaag gctcaaataa agacaataaa
    19621 gccaaaaatt atgctccaga tgaagaacct gaagattcag ggaaagtaac tcacaactat
    19681 ggtttttatg atgttaataa aggttcagac gaagaagaac cagaaaaaca agaagatgaa
    19741 tcagagctag atgaatatga actaggaatg gcacaaaacg ctaagaaata tggtatggat
    19801 agacaatctt ttgaaaagca actcatccaa ttatcaaata aatatagtgt aagttttgaa
    19861 agctttaatt atattaatgg tagtcaagtt caagttacta aaaaagatgg ctctaaagtg
    19921 cttgttgata ttaaaacact aacagaagtg aaataataaa agagttgagt aaacttagct
    19981 gacaactaaa aacaaacaag tcaccgtact tgtttgtttt ttagttatga tttctagcgt
    20041 gcgaagcttt taatgatata tctaattctt cttttgaaca accattgttt tacgcttaaa
    20101 aagagagtta atatattttt gtttgaggaa gttttcttgt tttgactttt ataaaacgac
    20161 taaagttata atcactaaat aaagagccac tactaaaagc tgtataacag gtttctagta
    20221 gtggctcact tgttttatta catgtaattg ttattagtta aaattttggt gaagctactg
    20281 tattgctcct taacaacatt atcaatatta tgatctgtta atacaaaatc aatatcgtct
    20341 aattgataaa aagtataaaa atcatattta ccaaattttt ggctatcaac taaaaggaat
    20401 ttttcaaagg aattattaag agcaatgcgc tgaatttcac cttctccctc gtcgtaagta
    20461 gcgatagact tttcaaaaac accattacta ctaacgaaag cttttgaaaa attaagtgat
    20521 ttaatagagt taatagtaac agaaccgaca aaagcaccag taatttctct gtactctccc
    20581 cctacaagga taaggtcaag tgttgaactt tgattcagaa tattgaaaac aggaaggcta
    20641 ttggttacta ccctaatttt aaaatcaagt aattgcgtgg ctaatttctc taaggttgtt
    20701 ccaggcccaa taaagattgt ttcaccatca ttgatgaatt gttttgcaaa ttgggcaatt
    20761 tctaattttt catttgtttg aatatcgtat ttttcagtgt ttgatttttc ataattttgt
    20821 ggagttggtg atgcatttac tttttgtgca ccaccatgga tgcgtattaa cagtcctgct
    20881 ttatctaatt catccaagtc acgcctaaca gtcatgtctg atacatcaag ttcttccatg
    20941 atttcattaa cagtgacaat gccatttata ttgacttttt ctattatttt ttgtaatctt
    21001 tctcgcttta acataatgac ctctcctac
//
LOCUS       DBJYSH010000022        20881 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.022, whole genome shotgun sequence.
ACCESSION   DBJYSH010000022 DBJYSH010000000
VERSION     DBJYSH010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 20881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20881
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.022"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            53..1603
                     /locus_tag="ACUBV3_000667"
     CDS             53..1603
                     /locus_tag="ACUBV3_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HHU3847193.1"
                     /translation="MEKTMDMEQREEVVSYNRAKVWQLVMFAMNNASTNIYLFTFMFV
                     TYFSTGVLGSAAIFVSQIMGYIRIFDGFIDPAIGIMIDKTDTKFGKYRPILIIGNVIT
                     ALSLIFLLALRGVDENIRFPLFILVLIIHKIGYSMQQTITKAGQTALTNDPKQRPIFN
                     IVDAVMTTSLMTGGQFVVSVFLVPKFGNFTPQFFNVLIFGTILISAILAIVAIIGIWA
                     KDRKEFFGLGENTQKTALKDYWKVLKGNKPLQILSIAAALVKFAIQFFGDSVVMVLLF
                     GILFGNYALSGQFSLLFIVPGVIINILFSTIARKKGLRFSYVRAIQIGMIGLLAFGAV
                     LYVGKPGDLSLTSLNLYTILFIVTNIIARYASQAPASLVLTMGADISDYETSESGRYV
                     SGMIGTIFSLTDSIASSFAPMVVGFVLAGIGFSKSFPTIETPLPPDLKMAAISILVAI
                     PFIALSIALLLMKFYKLDKEEMVRIQEKIQVMKAATTKERAKDIARNVPLTDMDYVDV
                     TKYPIDKD"
     gene            1670..2695
                     /locus_tag="ACUBV3_000668"
     CDS             1670..2695
                     /locus_tag="ACUBV3_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HHU3847194.1"
                     /translation="MAKIISLGEVLLRLSPPQYHTLMQANHLKCQFGGSELNVLASLA
                     QLGYHVGLVSALPDNDLGKMASQFILSQQISPAAIIKKEGRLGIYYYEQGFSVRTNKV
                     IYDRNYSSFWESTLSDYDFTSIFKGVDWFHVSGITPALTKDLYEVTRFLMTKAKEGGV
                     KVSIDLNFRESLWSSFQEAREQLSPLLGLLDVCFGLEPIYLAGESEDLKDELGLSRPY
                     LDIELLEKITQKIVQEYGLDYIAFTQREMEYTNQYMLKSYLYHNNMLYQTDKTGVEVL
                     DRVGTGDAFAAGLIHALLEKETPQRALEIAMATFKYKHTIQGDINIMTRDDIAYLIEK
                     ETNDIKR"
     gene            2712..4511
                     /gene="uidA"
                     /locus_tag="ACUBV3_000669"
     CDS             2712..4511
                     /gene="uidA"
                     /locus_tag="ACUBV3_000669"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HHU3847195.1"
                     /translation="MLYPLLTKTRNTYDLGGIWNFKLGEHNPNELLPSDEVMVIPTSF
                     NDLMVSKEKRDYIGDFWYEKVIEVPKVSEDEEMVLRFGSVTHQAKIYVDGVLVGEHKG
                     GFTPFEVLVPECKYNNEKIKVSICANNVLDYTTLPVGNYSEIIQEDGSIKKKVRENFD
                     FFNYAGVHRPLKLMIRPKNHIFDITITSRLSDDLQSADLHFLVETNQKVDEVRISVFD
                     EDNKLVGETKDSRLFLSDVHLWEVLNAYLYTARVEIFVDNQLQDVYEENFGLREIEVT
                     NGQFLLNRKPIYFKGFGKHEDTFINGRGLNEAANLMDLNLLKDMGANSFRTSHYPYSE
                     EMMRLADRMGVLVIDEVPAVGLFQNFNASLDLSPKDNGTWNLMQTKAAHEQAIQELVK
                     RDKNHPSVVMWVVANEPASHEAGAHDYFEPLVKLYKDLDPQKRPVTLVNILMATPDRD
                     QVMDLVDVVCLNRYYGWYVDHGDLTNAEVGIRKELLEWQDKFPDKPIIITEYGADTLP
                     GLHSTWNIPYTEEFQCDFYEMSHRVFDGIPNLVGEQVWNFADFETNLMILRVQGNHKG
                     LFSRNRQPKQVVKEFKKRWMTIPHYHNKKNSVK"
     gene            4540..5211
                     /locus_tag="ACUBV3_000670"
     CDS             4540..5211
                     /locus_tag="ACUBV3_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HHU3847196.1"
                     /translation="MARPLVEQTADRLLHLILEREYPVGAKLPNEYELAEDLDVGRST
                     IREAVRSLATRNILEVRQGSGTYISSKKGVSEDPLGFSLIKDTDRLTSDLFELRLLLE
                     PRIAELVAYRITDDQLQLLEKLVGDIEDAVHAGDPKHLLLDVEFHSMLAKYSGNIAMD
                     SLLPVINQSIHLINANYTNRQMKSDSLEAHREIIKAIREKNPVAAHDAMLMHIMSVRR
                     SALKA"
     gene            5292..5945
                     /locus_tag="ACUBV3_000671"
     CDS             5292..5945
                     /locus_tag="ACUBV3_000671"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008675 -
                     2-dehydro-3-deoxy-phosphogluconate aldolase activity
                     [Evidence IEA];
                     GO_function: GO:0008700 - (R,S)-4-hydroxy-2-oxoglutarate
                     aldolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HHU3847197.1"
                     /translation="MVKDNGYILGGIMLNQLKNNYFFAVIRGKSSEDALEIAKHAILG
                     GIRNIEVTFSTPEASKVIKQLSDDFKNNKEIIVGAGTVMTTELAKEAIDAGAKFLVSP
                     HFDSDIANLANENKVYYFPGCATATEIVVARKYKCQIIKLFPGGVVGPGFIKDIHGPI
                     PDVDLMPSGGVSVSNVVEWRKAGAVAVGVGSALSSKVATEGYDSVTKIAKQFVSALD"
     gene            5962..7362
                     /gene="uxaC"
                     /locus_tag="ACUBV3_000672"
     CDS             5962..7362
                     /gene="uxaC"
                     /locus_tag="ACUBV3_000672"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HHU3847198.1"
                     /translation="MAFNTETFMLKNQAAIQLYEEVKRQPIFDYHCHLDPKDIFEDHI
                     FDNIVDLWLGGDHYKWRLMRANGISEAEITGPASNLEKFKAFARTLERAYGNPVYHWS
                     AMELKNIFGVNEILTESNAEEIYHRLNHFLKEHKISPRRLIADSKVMFIGTTDHPLDT
                     LEWHKKLAADESFKTVVAPTFRPDEAFIEHRHFVDFITKLGDITQKEITDFSTFIAAM
                     EERIAYFAQNGCRASDISFTEIVFEQTDELELNDLFNKVCEGYIPNQSEISKWQTAVF
                     MELCRLYKKYGFVTQVHFGALRNNHSTIFEKLGADVGVDSLGDQVALTVNMNRLLDSL
                     VKKDSLPKMIWYNLNPAYNIAVANTLANFQANELGVRSYLQFGAGWWFADTKLGMISQ
                     MNALAEQGMLANFIGMLTDSRSFLSYQRHDYFRRILCTYLGEWIEEGEVPEDYQALGS
                     MAKDIAYQNAVNYFKN"
     gene            7380..8426
                     /gene="uxuA"
                     /locus_tag="ACUBV3_000673"
     CDS             7380..8426
                     /gene="uxuA"
                     /locus_tag="ACUBV3_000673"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HHU3847199.1"
                     /translation="MEMSFRWYGEDDPVTLENIGQIPTMKGIVTAIYDVPVGEVWSRE
                     RIQQLKEKVEAAGLKISVIESVPVHEDIKLGRPTRDLLIDNYIQTVKNLAAEGIDTIC
                     YNFMPVFDWTRTDLAYQYPDGSTALIFDETVSKKMDPVNGELSLPGWDASYSKEEMKA
                     IMDAYAEIDEEKLWENLTYFIKRIIPEAEAVGVKMAIHPDDPPYSIFGLPRIITGLEA
                     IERFVKLYDSKSNGITLCVGSYASDPQNDVLEISRRAFELDRVNFVHARNIKLGDGKS
                     FKESAHPSEYGSIDMYEVIKLCHEFGFEGAIRPDHGRMIWGETGRPGYGLYDRALGAT
                     YVSGLYEAVIKGSK"
     gene            8550..9389
                     /locus_tag="ACUBV3_000674"
                     /pseudo
     CDS             8550..9389
                     /locus_tag="ACUBV3_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000047575.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            9542..10354
                     /locus_tag="ACUBV3_000675"
     CDS             9542..10354
                     /locus_tag="ACUBV3_000675"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HHU3847200.1"
                     /translation="MVDTNHKIAFCIDSDGCAMDTMTYKHQLFFGPIAADIFGISEQE
                     RTSFLKEWDRVNLYSRTRGVNRFVGLVMGLEFAGVEHIDKLKEWVETTDSLSNNSLEL
                     ALANDFSRDLEKALEWSNEVNRQIKNYEGDSLAFSGVLESLERLSQFGNVYVVSSANK
                     EAVWQEWEEQGLLKYVDDIYCQDRGKKEDVIATLIASGYCRETILMVGDSLGELAAAE
                     QNGIAFYPVLVGKEVKSWEILREDIGEAFAKGQFEQQRQKESINTFWANLDN"
     gene            10370..12160
                     /locus_tag="ACUBV3_000676"
     CDS             10370..12160
                     /locus_tag="ACUBV3_000676"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000149450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HHU3847201.1"
                     /translation="MTHLVDLTKKPFNLNQEAIEWIEKTINEMTLDEKIGQLFFNMGA
                     SRSEEYLTDVLDRYHIAAVRYNRGSSSEIYDQNLILQTKSKLPMLIAANTEAGGDGAV
                     TDGTKVGDEIKVAATNDPKYAYEMGRIAGMEASAVGCNASFSPIVDLTRNWRNPIIAS
                     RNWGANVDQIISLSKEYMKGIMQYNIVPFAKHFPGDGIDERDHHLSFASNPMSKEEWM
                     STFGRIYGELADAGLPGVMAGHIHLPNVEKEMHPERDLDDMLPASLNKTLLDELLRGE
                     LGYNGAIVTDASHMVGMTASMPRRDLLPTAIEAGCDLFLFFNDPDEDIQWMKEGYEKG
                     ILTEERLHDALRRTLGLKAKLGLHNYEGRRQELFMPKDKAMALINTLESQKIADEVAD
                     KAVTLVKDKQKDIFPVNPERYRHILLVNVEGYKGGFGAMIAGNKQRASDYMKELLEAR
                     GHEVTVWESTEERIMKLPQEERAAAIANVYAQKQPIANLTEHYDLIINLVDVNAGGTT
                     QRIIWPAAKGTPDQPFYVHEIPSIVISVQHAFALADMPQVGTYINAYDGLPSTISAVV
                     AKLAGESEFTGVSPVDAYCGLIDTHLWRQC"
     gene            complement(12218..13303)
                     /locus_tag="ACUBV3_000677"
     CDS             complement(12218..13303)
                     /locus_tag="ACUBV3_000677"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HHU3847202.1"
                     /translation="MSKLNRIRHHLHSVQAELAVFSDPVTVNYLTGFFCDPHERQMFL
                     FVYEDRDPILFVPALEVSRAKQSVPFPVFGYIDSENPWQKIASNLPSFSVSKVLAEFD
                     NLNVTKFQGLQTVFDGHFENLTPYIQNMRLIKSRDEIEKMLVAGEFADKAVQVGFDNI
                     SLNNTETDIIAQIEFEMKKQGINKMSFDTMVLTGNNAANPHGIPGTNKIENNALLLFD
                     LGVETLGYTSDMTRTVAVGKPDQFKKDIYHLCLEAHQAAIDFIKPGVLASEVDAAARN
                     VIEKAGYGQYFNHRLGHGLGMDVHEFPSIMAGNDMEIQEGMCFSVEPGIYIPDKVGVR
                     IEDCGYVTKTGFEVFTKTPKELLYFEG"
     gene            13513..14517
                     /gene="ccpA"
                     /locus_tag="ACUBV3_000678"
     CDS             13513..14517
                     /gene="ccpA"
                     /locus_tag="ACUBV3_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HHU3847203.1"
                     /translation="MNTDDTITIYDVAREAGVSMATVSRVVNGNKNVKENTRKKVLEV
                     IDRLDYRPNAVARGLASKKTTTVGVVIPNIANSYFSILARGIDDIAAMYKYNIVLASS
                     DEDDDKEVNVVNTLFAKQVDGIIFMGHHLTEKIRAEFSRSRTPIVLAGTVDLEHQLPS
                     VNIDYKAAAVDVIDILAGNHKDIAFVSGPLIDDINGKVRLAGYKEGLKKNGLNFKEGL
                     VFEANYRYAEGFALAQRVINAGATAAYVAEDELAAGLLNGLFEAGKRVPEDFEIITSN
                     DSPIAQYTRPNLTSISQPVYDLGAVSMRMLTKIMHKEELEEKEIVLNHGIVKRGTTNN
                     "
     gene            14649..16115
                     /locus_tag="ACUBV3_000679"
     CDS             14649..16115
                     /locus_tag="ACUBV3_000679"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HHU3847204.1"
                     /translation="MTNELIMQAFEWYLPSDGNHWKKLEESISDLKKLGISKIWLPPA
                     FKGTSSDDVGYGVYDLFDLGEFDQNGTIRTKYGRKEEYLKLIKSLKANGIKPFADIVL
                     NHKANGDHKEKFQVIKVNPENRQEALSEPYEIEGWTGFDFPGRQGEYNDFKWHWYHFT
                     GLDYDAKNNETDIFMIVGDNKGWADDDLIDDENGNFDYLMYNDIDFKHPEVIKNLQDW
                     AKWFIETTGIEGFRLDAVKHIDSYFIQTFINDIRTKIKPDLEVFGEYWKSDQTSMKDY
                     LEATQFQFSLVDVTLHMNFFDASHQNRDFDMRTIFDDSLVIDNPEYAVTFVENHDTQS
                     GQALESRVEDWFKPLAYGLILLRQQGTPCLFYGDYYGIQGEFGQPSFKEVIDKMAELR
                     QNYVFGEQVDYFTHSNCIGWTCLGDEEHNSCLAVVLTNGDQGWKHMEVGEIYAGKTFV
                     DYLGNCEQEVVIGDDGWGDFLVASASISAWVPKIEEAN"
     gene            16160..17158
                     /locus_tag="ACUBV3_000680"
     CDS             16160..17158
                     /locus_tag="ACUBV3_000680"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HHU3847205.1"
                     /translation="MKVLLYLEAEEYLKKSGIGRAIKHQEKALQIAGIDYTTNPTDDF
                     DLVHMNTYGIRSWLLMSKAKKTGKKVIMHGHSTEEDFRNSFIGSNLVSPLFKWYLCRF
                     YQKADAIITPTDYSKQLIKAYGIKKPIFVLSNGIDLSRYQRSEKKEAAFRHYFHLSKD
                     DKVVMGAGLYFMRKGIDQFVEVAAKMPDIRFIWFGETNKWVIPRKVRQIVTKQHPSNV
                     TFAGYIKGDVYEGAMSASDAFFFPSREETEGIVVLEALASHQHVVLRDIPVYHGWVTE
                     DSVELATDVDGFVEKLDKVLSGKSDKIKEGYRVAESRSIERIAHELASVYQKVMEL"
     gene            17160..18494
                     /locus_tag="ACUBV3_000681"
     CDS             17160..18494
                     /locus_tag="ACUBV3_000681"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HHU3847206.1"
                     /translation="MRIGLFTDTYFPQVSGVSTSIRTLKEGLEKEGHEVYIFTTTDRN
                     VKRFEDPTIIRLPSVPFISFTDRRVVYRGLISAYRIAKDYELDIIHTQTEFSLGLLGK
                     LVAKALRIPVVHTYHTQYEDYVGYIAKGKLIKPSMVKYIMRTYLSDLDGVICPSRIVL
                     NLLDGYGVKIPKQVIPTGIPVENYRREDISEETIKNLRTELGLADNDTMLLSLSRVSF
                     EKNIQAILMHLSAVVDENPHVKLVIVGDGPYLSDLKELVHSLELENSVIFTGMVEHSQ
                     VAIYYKACDFFISASTSETQGLTYIESLASGRPIIAQSNPYLDDVISDKMFGTLYKKE
                     SVLADAILDAIAETPKMTQEAYEQKLYEISAENFSKSVYAFYLDFLISQKASVKEKVS
                     LTIGNKDSHSTLRFVRKAVYLPKKVFTFTGRASKKVVKAPKRRISSIRDFLD"
     gene            18951..>20881
                     /gene="thrS"
                     /locus_tag="ACUBV3_000682"
     CDS             18951..>20881
                     /gene="thrS"
                     /locus_tag="ACUBV3_000682"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HHU3847207.1"
                     /translation="MIKITFPDGAIREFESGITTFEIAQSISNSLAKKALAGKFNGQL
                     IDTTRAIEEDGSIEIVTPDHEDALGVLRHSAAHLFAQAAKRLFPNLCLGVGPAIHDGF
                     YYDTDNKSGQISNDDLPRIEEEMKKIVKENHPCIREEISKEEALELFKDDPYKVELIS
                     EHAEDGLTVYRQGEFVDLCRGPHVPSTGRIQVFHLLNVAGAYWRGNSDNAMMQRVYGT
                     AWFDKKDLKAYLKRREEAKERDHRKLGKELDLFMVNPEVGQGLPFWLPNGATIRRELE
                     RYIVDKEIASGYQHVYTPPMASVEFYKTSGHWDHYREDMFPTMDMGDGEEFVLRPMNC
                     PHHIEVYKHHVHSYRELPIRIAELGMMHRYEKSGALTGLQRVREMTLNDAHIFVTPEQ
                     IKDEFLKALNLIAEIYEDFNLTDYRFRLSYRDPEDKHKYYDNDEMWENAQAMLKEAMD
                     DFGLDYFEAEGEAAFYGPKLDIQVKTALGNEETLSTIQLDFLLPERFDLKYIGADGEE
                     HRPIMIHRGGISTMERFTAILIETYKGAFPTWLAPQQVSVIPISNEAHIDYAWEVARA
                     LKDRGIRAEVDDRNEKMQYKIRAAQTQKIPYQLIVGDKEMEEKAVNVRRYGSKATETK
                     SIEEFVESILADIARKSRP"
BASE COUNT         6703 a         3218 c         4379 g         6581 t
ORIGIN      
        1 atagtattat ttaatcataa aagcgtttac aaaatacaag gagggttctc tcatggagaa
       61 aacaatggat atggaacaac gtgaagaagt tgtatcttat aaccgagcga aagtatggca
      121 gttagttatg tttgccatga acaatgcttc tacaaatata tacttattta cgtttatgtt
      181 tgttacctat ttctcaactg gcgttcttgg ctcggcagcc atttttgtca gtcaaattat
      241 gggttatatc cgtatttttg atggttttat tgatccagct attggtatta tgattgataa
      301 aactgataca aaatttggta agtatcgtcc tattttgatt attgggaacg ttattacggc
      361 tttgtcattg atttttcttc ttgcattgag aggtgtcgat gaaaatattc gtttcccatt
      421 atttatcctt gttctaatta tccataaaat tggatactca atgcaacaga caataactaa
      481 agcagggcaa acagcactga caaatgaccc taagcaacgt cctattttta atatcgttga
      541 tgcagttatg acaacatcat taatgacagg cggtcagttt gtcgtttcag ttttcttagt
      601 accaaaattt ggtaatttta cacctcaatt cttcaatgtc cttatttttg gaacaattct
      661 tatttcagct attttagcaa tcgttgcaat tattgggatt tgggcaaaag acagaaaaga
      721 attctttggt ttaggagaga atacccaaaa gactgctcta aaagattact ggaaggtgtt
      781 gaaaggaaat aaaccgcttc agatattatc cattgctgca gctttggtaa aatttgccat
      841 ccaattcttt ggcgattccg ttgttatggt gctattgttt ggtattttat ttggtaatta
      901 tgctttatca ggacaattct cacttctttt cattgttcct ggtgttatta ttaatatttt
      961 gttctcaaca attgctcgta aaaaaggtct tcgtttttca tatgtcagag caattcagat
     1021 cggtatgatt ggtttacttg cttttggtgc agttctttac gttggtaagc ctggtgacct
     1081 tagtttaaca agcctaaatc tttatactat tttatttatc gttactaata ttattgcgag
     1141 atacgcttca caagcacctg ctagtttagt attgactatg ggagcagata tttctgacta
     1201 tgaaacttct gaatccggtc gttatgtatc aggaatgatt ggaactatct tctccttaac
     1261 agattcgatt gcttcttcat ttgctcctat ggtagtaggt tttgtgttag caggaattgg
     1321 attttcaaaa tcatttccaa ctatcgaaac accactgccc cctgatttaa aaatggcagc
     1381 tattagtatc cttgtggcca ttccatttat agctttgtct atagctcttc ttttaatgaa
     1441 attttacaaa ctagataaag aagaaatggt tcgtattcaa gagaagattc aagttatgaa
     1501 agcagcaact actaaggaac gtgctaaaga tattgctcgt aatgttcctt tgacagatat
     1561 ggattatgtt gatgtaacaa aatatcctat tgataaagat taaaaagttt agctgctctt
     1621 caatagagca gctattttgt taaataagag ctaccattgg aggattctta tggcaaaaat
     1681 tatttcatta ggtgaggttc ttttgcgatt atctccacct caatatcaca cactgatgca
     1741 ggctaatcac ctaaaatgcc aattcggtgg ttcagaactt aatgtcttag caagccttgc
     1801 tcaactaggt taccatgtag gattggtctc agctttgcct gataatgatt tgggcaagat
     1861 ggcaagtcaa tttatattgt cacagcaaat tagtccagct gctataatta aaaaagaagg
     1921 acgactagga atttattact atgaacaagg attttcggta agaacaaata aggttatcta
     1981 cgatcgtaac tattcatctt tttgggaaag taccttgagt gattatgatt ttacttctat
     2041 ttttaaggga gtggattggt ttcatgtttc tggaattact ccagcactaa ccaaggactt
     2101 atatgaggtg actcggttcc ttatgacaaa agctaaagaa ggaggtgtaa aagtatcgat
     2161 tgatctcaat tttcgtgaga gtttatggtc atcttttcaa gaggcacgtg agcaattatc
     2221 gccactatta ggattattag atgtttgctt tggcttagag ccgatttatt tagctggcga
     2281 aagtgaagat ttaaaagatg agttgggctt atccagacct taccttgata ttgaattact
     2341 tgaaaagata acccaaaaga ttgtccaaga atatgggtta gactatattg cctttactca
     2401 aagggaaatg gaatatacta atcagtatat gttaaaatca tatttgtatc ataataatat
     2461 gttatatcaa actgataaaa ctggcgttga agtattagat cgtgtgggga caggagatgc
     2521 ctttgctgca ggtttaattc atgcgcttct ggaaaaagaa actcctcaaa gggccttaga
     2581 aatagctatg gctacattta aatacaaaca taccattcaa ggagatatca atatcatgac
     2641 acgagatgat attgcttacc tcattgaaaa agaaacaaat gatattaaac gatagtgtaa
     2701 aaggagagtg tatgttatat ccattattga caaaaacaag aaatacttat gatttaggcg
     2761 gtatttggaa ctttaaatta ggagaacata atccaaatga attactacct tcagatgaag
     2821 tcatggttat cccgacttca tttaatgatt tgatggtaag taaagaaaaa cgtgattata
     2881 taggtgattt ttggtatgaa aaagtcattg aagtacctaa agtatcagag gatgaagaga
     2941 tggtgctgcg ttttggctca gtgacacatc aagctaaaat ttatgttgac ggtgtattgg
     3001 taggagagca taagggaggt tttactcctt ttgaagttct tgttcctgaa tgtaagtata
     3061 ataatgagaa aattaaggta tcaatttgtg ctaataacgt tttagactat accacgcttc
     3121 cggtcggaaa ttatagtgaa atcattcaag aagatggtag cattaagaag aaagtacgtg
     3181 agaattttga tttcttcaat tatgcaggtg tgcatcgtcc tcttaagttg atgatacgtc
     3241 ctaaaaatca tatttttgat attacaatta caagtcgtct atcagatgat ttacagtcag
     3301 cagatttaca ttttttagtg gagactaatc aaaaggttga cgaagttaga attagcgttt
     3361 ttgatgaaga taacaaacta gtgggtgaaa cgaaggatag cagattattt cttagtgatg
     3421 tccatctttg ggaagtttta aatgcctatc tgtacacagc gcgtgttgaa atttttgttg
     3481 ataatcaact ccaagatgtc tatgaggaaa attttggtct tagagaaata gaagtgacaa
     3541 atggtcaatt cctattgaat cgcaaaccta tttattttaa aggatttggt aaacatgaag
     3601 atacgttcat taatggcaga ggtttgaatg aagctgctaa cttaatggat ttgaatcttt
     3661 tgaaggatat gggggcaaac tcttttagaa catcccatta tccttattct gaagaaatga
     3721 tgcgtttagc agatcgaatg ggagtgttag tgatagatga ggttccagca gtagggttat
     3781 ttcaaaattt taacgcttcc ttagatttgt caccaaaaga taatggtacg tggaatttga
     3841 tgcaaacaaa agcggcgcat gaacaagcta tccaagaatt agtgaagcgt gataaaaatc
     3901 atcctagcgt cgtgatgtgg gtagtcgcta acgaaccggc tagtcatgaa gcgggagcac
     3961 atgattattt tgagccatta gtaaaacttt ataaagattt agaccctcaa aaacgtcctg
     4021 tcaccttggt taatatttta atggcaaccc cagatagaga ccaagtgatg gacctggttg
     4081 atgttgtctg ccttaatcgt tactatggtt ggtacgttga ccacggtgat ttaacaaatg
     4141 cagaagtagg tataagaaaa gagttattag aatggcaaga taaatttcct gacaaaccaa
     4201 ttatcataac ggagtatggc gctgatacgt taccgggatt acattctact tggaatattc
     4261 catatacaga agaatttcaa tgtgattttt atgaaatgag tcatcgtgtt tttgatggta
     4321 ttcctaattt agttggtgag caagtctgga attttgcaga ctttgaaact aatctgatga
     4381 tacttcgtgt acaggggaat cacaaaggtc tcttttcaag gaatcgccag ccgaaacaag
     4441 tcgtcaaaga atttaaaaaa cgctggatga ctattcctca ttaccataat aaaaaaaata
     4501 gtgtaaaata ggggtagtta ttttagaaga ggtgatgata tggctagacc cttagtagaa
     4561 caaacagcag atcgtctatt gcatttaatt ttagaaagag aataccctgt tggtgctaag
     4621 ctccccaatg aatatgaatt agcagaagat ctagatgttg gtcgcagcac aatccgagaa
     4681 gcagtcagaa gtttagcaac gcgtaatatt ttagaagtca gacagggctc tggaacatat
     4741 attagttcta aaaagggtgt ttctgaggat ccattagggt tctctctaat aaaggacaca
     4801 gataggttga catcagatct ctttgaacta cgccttttat tggaacctcg aatagcagag
     4861 ctggtagctt atagaattac agatgatcag ttacaattac tcgaaaagct tgttggagat
     4921 attgaagatg ctgttcacgc aggcgatccg aaacatttac ttttagacgt ggaatttcat
     4981 tccatgctag ctaaatacag tggtaatatt gcaatggata gccttttgcc agtcatcaac
     5041 cagtcaattc atctgataaa tgcgaattat acaaatcgtc agatgaagtc ggatagttta
     5101 gaagcacata gagaaattat caaagcaatc cgagaaaaaa atccagtagc tgcacatgat
     5161 gctatgctta tgcatatcat gagtgttaga cgttcagcgt tgaaagctta atataagaag
     5221 aaaaatagct attcttgaat agctattttt ttagtattct tcttgacaat tcatcagatg
     5281 acttatataa tatagttaaa gataacggtt acattttggg aggtattatg ttaaatcaat
     5341 tgaaaaacaa ctatttcttt gctgttattc gagggaaatc atctgaggat gcgctcgaaa
     5401 tcgcaaagca tgctattttg ggtggtattc ggaatattga agtaactttt tcgactcctg
     5461 aagcaagcaa agtcatcaaa caattatcag atgactttaa aaataataaa gaaattattg
     5521 tgggggccgg aacggtcatg acaactgaac ttgctaaaga ggctattgat gctggagcta
     5581 aatttttggt tagcccacac tttgattcag atattgctaa tttagctaat gaaaacaagg
     5641 tttattattt cccaggctgt gcaacggcaa cagaaatcgt ggtagctaga aagtataaat
     5701 gtcagattat aaagcttttt ccaggtggag ttgtgggtcc aggttttatc aaagatattc
     5761 atggtcctat tccagatgtt gatttaatgc catcaggtgg cgtctctgtt tcaaatgtag
     5821 ttgaatggag aaaagcagga gcagttgcgg ttggtgttgg ctcagcatta tctagtaaag
     5881 tagccacaga aggctatgat agtgttacta aaatagctaa acagtttgtt tctgcacttg
     5941 attagtgagt aagagaagaa tatggcgttt aatactgaaa catttatgtt aaaaaatcaa
     6001 gcagctatac agctgtatga agaggtcaag agacaaccta tttttgatta tcattgccac
     6061 ttagatccaa aggacatttt tgaagatcat atttttgata atattgtgga tttgtggctt
     6121 ggtggtgacc actataaatg gcgtcttatg agggctaatg gtatttctga agcagaaatt
     6181 acaggtccag catccaacct tgagaaattt aaagcatttg ctagaacact agaaagagca
     6241 tacggtaatc ctgtttatca ttggtctgct atggaattga aaaatatctt tggtgttaat
     6301 gagattttga cagaatcaaa tgctgaggaa atttatcatc gtttaaatca tttcttgaaa
     6361 gagcataaaa taagtcctag acgtttaatt gcagatagca aagttatgtt tattggtact
     6421 acagaccacc ctcttgatac tctcgagtgg cataaaaagt tggctgctga tgagagtttt
     6481 aaaacagttg ttgctccgac atttaggcca gatgaagctt ttattgaaca tcgacatttt
     6541 gttgatttta ttactaaact gggtgacatt actcaaaaag agattacaga tttttcaaca
     6601 tttatagcag caatggaaga acgcattgcg tattttgctc aaaatggttg tagggctagc
     6661 gatatcagtt ttacggaaat cgtctttgaa caaactgatg aattggagtt gaacgattta
     6721 tttaataaag tttgtgaagg ctatatccca aatcaatcag aaatcagcaa atggcaaaca
     6781 gctgttttta tggaactttg ccgattatat aaaaaatatg gttttgtaac acaggtgcat
     6841 tttggagctt tgagaaataa tcattctact atttttgaaa aattgggagc tgacgtgggt
     6901 gtggattcac ttggtgacca agtggcttta actgttaata tgaaccgttt attggattcc
     6961 ttggttaaaa aagatagtct gcctaaaatg atttggtata atctcaatcc agcttacaat
     7021 atagcagtag ctaacacttt ggcaaatttt caagctaacg aacttggagt gagatcttac
     7081 cttcaatttg gtgcaggttg gtggtttgca gatactaaat tagggatgat tagtcaaatg
     7141 aatgccttag cagagcaagg catgttggcg aattttattg gtatgttaac agattctaga
     7201 agttttctat cttatcaacg tcatgactat tttcgacgta ttttatgtac ctacttagga
     7261 gaatggattg aagaaggaga agttccagaa gattatcaag ctttaggaag tatggctaaa
     7321 gacatcgctt atcaaaatgc agttaattat tttaagaact aaaaaggaag agtgaaatca
     7381 tggaaatgtc atttaggtgg tatggtgaag acgaccccgt tactttagaa aatattggtc
     7441 aaattccaac aatgaaaggg attgttacag cgatatacga tgtaccagta ggagaagttt
     7501 ggtcacgtga gcgcattcaa cagctgaaag aaaaggtaga ggcagcaggg cttaaaattt
     7561 cggttatcga atcagtacct gttcatgaag atatcaaatt gggacgtcca acacgtgatc
     7621 tcttgattga taattatatt caaaccgtca aaaatttggc tgcggaaggt attgatacta
     7681 tttgttataa ttttatgcca gtatttgact ggacacgcac tgatttagca tatcaatatc
     7741 cagatggttc cacagctttg atatttgatg agacggtttc taaaaaaatg gatccagtaa
     7801 acggtgaatt atctttgcca ggatgggatg ccagctatag taaagaagaa atgaaggcta
     7861 tcatggatgc ctatgctgaa attgatgagg aaaaactttg ggaaaatctc acttatttta
     7921 ttaaacgtat tattcctgaa gcggaagccg taggcgttaa aatggccatt catccggatg
     7981 acccaccata ttctattttt ggtttacctc gcattattac tgggttagag gctattgagc
     8041 gttttgtgaa attgtacgat agtaaaagta atggcatcac tttatgtgtg ggttcttacg
     8101 cttctgatcc ccagaatgat gttttagaga tttcgcgacg tgcttttgag ttagatcgtg
     8161 taaactttgt tcatgctcgc aatattaaat taggtgatgg aaagtcattt aaagaatcgg
     8221 ctcatccatc agaatacggg tcaatagaca tgtatgaagt tattaagtta tgccatgaat
     8281 ttgggtttga gggtgctatt cgacctgacc acgggcgtat gatttgggga gaaacaggtc
     8341 gtccaggata tggcctatac gatagagctt taggagccac ttacgtttct ggtttatacg
     8401 aagctgtgat taaaggttca aaataaggcg atgcttccaa atttctcctg ttccaatctc
     8461 ctgaaagaga aatttggaaa gttattatca taatatgatt gtacttagct aagagtagaa
     8521 tacttataga agttaatgag aggaataaga tgtcaaaaac aattacattt accaataaag
     8581 ttgtcgtcat aacaggtgca ggcggtgttc tctgtggtta tatggctaaa gaatttgcga
     8641 aagcaggcgc taaagtggca ttattagatt tgaatcaaga agctgctcaa acatttgctg
     8701 atgaaattgt agaagaaggt ggtatcgcta aggcttataa agctaacgtt ttgtctaaag
     8761 aaaaccttga agaagtgcat caagccgttt tagaggatct tggaccaact gatattttag
     8821 ttaatggtgc aggaggcaac aatcctaaag ctacaactga taatgaattt cacgaattag
     8881 acttaccttc agaaacaaaa accttctttg agttagatga agctggtatt agctttgttt
     8941 ttaacttaaa ttatttagga acattattgc caacgcaagt atttgcccaa gatatggttg
     9001 gacgtgaagg agcgaatatt attaatatct cttcaatgaa tgctttcacc cctctgacca
     9061 aaataccagc ttattctgga gctaaagcag ctattagtaa cttcacacaa tagctagctg
     9121 ttcatttctc aaaagttggt atccgttgta atgctattgc acctggtttc ttggtaacaa
     9181 atcagaaccg aagcttgtta tttactgagg acggtcagcc aactgcgcgt gctgagaaaa
     9241 ttcttaataa tacaccaatg ggaagatttg gagaagcaag tgagctgatt ggcggattgt
     9301 tctttttagc agatgaaaaa tcatcaagtt ttgttaatgg cgttgtcctt ccgattgatg
     9361 gtggatttgc tgcttactct ggtgtttaat ttacaagata atagccactt aacacataag
     9421 agattattga gttgagtggt tttttaagga aatttgttta atgatcttac cactaaatga
     9481 tgggtaggtt gctaattgga aaatcctcat aagttagata aatttagaaa ctggagataa
     9541 tatggtagat acaaatcaca aaatcgcttt ttgcatagat tctgatggtt gcgcaatgga
     9601 tacaatgaca tataagcatc aactcttttt tggtccaatt gctgcagata tatttgggat
     9661 atcagaacaa gaaagaacaa gttttttaaa agagtgggat agagtcaatc tttattcaag
     9721 aactcgtgga gttaatcggt ttgtgggatt ggttatgggc cttgagtttg ccggagtaga
     9781 acatattgat aaattaaaag aatgggtcga aacaacagac tcattgtcca ataattcctt
     9841 agagttagcg cttgccaacg attttagtag agatttagag aaggctttag aatggtccaa
     9901 tgaagtgaat cgccaaatca aaaattatga gggagattct ttagcgtttt caggtgtgtt
     9961 agaaagttta gaacgattaa gtcaatttgg taatgtttat gtcgtgagtt ctgctaacaa
    10021 ggaagctgtt tggcaagagt gggaagaaca gggattgcta aagtatgttg atgatatcta
    10081 ttgtcaagat agaggtaaaa aagaagatgt tatcgccact ttaattgcaa gtggttactg
    10141 ccgtgaaact attttgatgg taggagattc cctgggtgaa ctggctgcag cagaacaaaa
    10201 tggtattgca ttttacccag tacttgttgg taaggaagtc aaatcatggg aaattcttag
    10261 agaagatatt ggtgaagctt ttgcaaaagg tcaattcgaa cagcaaaggc aaaaagaatc
    10321 tattaatacc ttttgggcta atttagataa ctaatagaaa ggataggcta tgacacattt
    10381 agttgactta acaaagaaac ccttcaatct aaatcaagaa gcaattgagt ggattgaaaa
    10441 aacaattaat gagatgacat tggatgagaa aattgggcaa ctttttttca atatgggagc
    10501 tagtcgttca gaagaatatt taacagatgt tttggatcgg tatcatattg ctgctgttcg
    10561 ctataataga ggtagttcaa gtgaaatcta tgatcagaat ttaattttac agacaaagtc
    10621 gaaattgccg atgttgattg ctgcaaatac agaagctggt ggagacggtg ctgtcacgga
    10681 tggaactaaa gttggcgatg aaataaaagt ggctgctaca aatgatccga aatatgctta
    10741 tgaaatggga agaatcgcag ggatggaggc atctgctgtg ggttgtaatg cttcattttc
    10801 tccaattgta gatttgacac gtaattggcg taatcctatc atagctagtc gaaattgggg
    10861 tgctaacgtt gatcaaatta tttcactttc aaaagagtat atgaaaggaa tcatgcaata
    10921 taatattgta cctttcgcta aacattttcc aggggatggc attgatgagc gtgatcatca
    10981 tttgtcattt gcttccaatc ctatgagtaa agaggagtgg atgtcgacat ttggccgcat
    11041 ttatggtgaa ttggctgacg caggtttacc aggagtcatg gcaggtcaca tacatttacc
    11101 aaacgttgaa aaagaaatgc atccagagcg agaccttgat gatatgttac cagcttcatt
    11161 gaataaaaca cttttagatg agttattgag aggagagtta ggttataatg gagctattgt
    11221 aacggacgct tctcatatgg ttggaatgac tgcttcaatg ccaaggagag atttgttgcc
    11281 aacggcgatt gaagcaggtt gtgatttatt cttattcttt aatgaccctg atgaagacat
    11341 tcaatggatg aaagaaggtt acgaaaaagg tattctaaca gaggaacgtc tccatgacgc
    11401 tttacgtcgt actctcggtc taaaagcgaa attaggcttg cataactatg agggacgtcg
    11461 ccaagaactc tttatgccta aagataaggc tatggcttta atcaacacat tagagtcgca
    11521 aaaaatagct gatgaagttg ctgataaagc agtaactctg gtaaaagata aacaaaaaga
    11581 tatctttcca gtaaatccag aacgttatcg tcatatctta cttgttaacg ttgaaggata
    11641 taagggaggt tttggtgcga tgattgctgg aaacaagcaa cgtgcatcgg attacatgaa
    11701 agagttactt gaggcacgtg gccatgaagt aactgtctgg gagtctaccg aagagcgtat
    11761 tatgaaattg ccacaagagg agcgggcagc agctatcgct aatgtttacg cacaaaaaca
    11821 gccaatcgca aatctaacag aacactatga cttgattatt aacttggtag atgtcaatgc
    11881 tggaggaaca acacaacgta ttatttggcc agcagctaaa ggcacccctg atcagccttt
    11941 ttacgttcat gaaataccat ctattgtgat ttcagtacaa catgcatttg ctttagcaga
    12001 tatgccacag gtagggactt atattaatgc ttatgatggg cttcctagca cgataagtgc
    12061 agtagttgcg aaattggctg gagaatcaga atttacagga gtttcaccag tagatgctta
    12121 ctgtggttta attgatacac atttatggcg ccaatgctaa tagaaacaag atgatgtact
    12181 tttatgtaac ataaaaaaag agcttaaagc tcttttttta tccttcaaaa tagagtagtt
    12241 ccttgggtgt tttggtaaat acttcaaagc cggttttagt cacataaccg cagtcttcaa
    12301 ttcgcacacc aactttatct ggtatataaa tacctggttc aacagagaag cacatgcctt
    12361 cttgaatctc catatcgtta ccagccataa tagatggaaa ttcatggaca tccataccta
    12421 atccatgacc aaggcggtgg ttaaagtatt gtccataacc agccttttca ataacatttc
    12481 ttgctgctgc gtcaacttca gaagcaagga ctcctggctt aataaaatca atagccgctt
    12541 gatgtgcttc aagacataaa tggtaaatat cttttttgaa ttgatcaggt ttccccactg
    12601 ctactgtacg ggtcatatct gacgtataac ctagtgtttc tacacctaaa tcaaataata
    12661 acaaagcatt attttcgatt ttattagttc ctgggatacc atgtggattt gctgcattat
    12721 tgccagtcaa aaccatagtg tcgaaactca ttttattgat accttgtttt ttcatttcaa
    12781 attcgatttg agcgataata tcggtttcag tgttgtttag cgagatattg tcaaatccaa
    12841 cttgaacagc tttatcagca aattctccag cgactaacat tttctcaatt tcatctcgtg
    12901 atttaattag gcgcatgttt tgaatataag gtgttaggtt ttcaaaatga ccatcaaaaa
    12961 cggtttggag accttggaat ttagtaacat taagattatc aaattcggct agaactttag
    13021 aaacagaaaa ggatggtaag ttgctagcta ttttttgcca aggattttct gaatctatgt
    13081 aaccaaagac gggaaatggt acactttgtt ttgcacgcga tacttctagt gctggtacaa
    13141 ataaaatagg atcgcgatct tcatagacaa ataaaaacat ttgtctttca tggggatcgc
    13201 aaaagaaacc tgttaaatag ttgactgtca caggatcaga aaagacagca agttcagctt
    13261 ggactgaatg taagtgatgg cgtatacgat taagttttga cataataacc cttcttccta
    13321 aaaggaactt agtctagtta ggctagtttc ttatgcctat tatttttcca aaaaaatagg
    13381 aaaaaatcaa gtttagttag tattttgaaa gtttttcata aaaaagcttt ataaaagctt
    13441 gaaagtgttt ccaaaaggtg ataaaatatc agtgaaagcg tcattttcaa atagaaagga
    13501 agttaaatca atatgaatac agatgatacg attacgattt atgatgttgc ccgtgaagca
    13561 ggtgtttcga tggcaactgt tagtcgtgtg gtaaatggaa ataaaaatgt caaggaaaat
    13621 acacgtaaga aagtccttga agttattgat cgtcttgact atcgtccaaa tgctgtggcg
    13681 cgtggtttag caagtaaaaa aacgacaact gttggtgttg tcattcctaa tatcgcaaat
    13741 agctactttt caattttagc gcgtggtatt gatgatattg cagcaatgta caaatataac
    13801 attgtccttg cttcaagtga tgaagacgat gataaagagg ttaatgttgt taatactttg
    13861 tttgctaagc aagtagacgg tattattttt atgggacacc acttaactga aaagattcgt
    13921 gcagagttct ctcgttcacg cacaccgatt gttttagcag gtactgtgga ccttgagcat
    13981 caattaccaa gtgttaatat cgattacaaa gcagcagcag ttgatgttat tgatatttta
    14041 gctggcaatc ataaagacat tgcttttgta tcaggaccac ttattgacga tattaatggc
    14101 aaggttcgtt tggcaggata caaagaaggt ctcaagaaaa atggcttaaa ctttaaagag
    14161 gggttggttt ttgaagccaa ctaccgttat gctgaaggct ttgcattagc tcaacgtgtg
    14221 attaatgctg gagcaacagc agcatatgtt gctgaggatg agttagcggc aggtttgtta
    14281 aatggtcttt ttgaggcagg taaacgtgtt cctgaagatt ttgaaattat cacaagcaat
    14341 gactcgccaa tcgcacaata tacacgtcct aacttaactt ctatcagtca accggtctat
    14401 gatttgggtg ctgttagcat gcgtatgcta acaaaaatta tgcacaaaga agagttggaa
    14461 gaaaaagaaa tagtgcttaa tcacgggatt gttaaacgtg gcacaacaaa taattagtgt
    14521 tagagtatgt tatttgaaaa atagtggtag aatggtagta gcttagcgct taactttctg
    14581 atacttaaca tgatatattg agaggattag ggtagcttag cttcctaatc tttttttaga
    14641 ggtgtaatat gacaaatgaa ttgataatgc aagcttttga gtggtattta cctagtgatg
    14701 ggaatcactg gaagaaatta gaggagtcta tatcagacct taaaaaactt ggaattagta
    14761 aaatctggtt accaccagca tttaagggaa ctagcagtga tgatgtagga tatggtgttt
    14821 atgatctctt tgatttagga gaatttgacc agaatggaac aattagaaca aaatatggta
    14881 ggaaagaaga gtatctaaag cttattaagt cgttaaaggc aaatggcatt aaaccgtttg
    14941 cagatatcgt tcttaaccat aaagccaatg gtgatcataa agaaaaattt caagtcatca
    15001 aagtcaatcc tgaaaatcgt caagaagcat taagtgaacc ctatgagatt gaaggatgga
    15061 cgggatttga tttcccaggt agacagggtg agtacaatga ttttaaatgg cactggtacc
    15121 actttactgg tttagattac gatgctaaaa ataatgaaac agacatcttt atgattgtag
    15181 gcgataataa aggttgggca gacgatgatt tgattgatga tgaaaatggt aatttcgatt
    15241 atctcatgta caatgacatt gattttaagc atcctgaagt tataaaaaac ttacaagatt
    15301 gggctaagtg gtttattgaa actacaggca ttgaaggttt tcgtttggat gctgtgaaac
    15361 atatcgattc ttactttatt caaactttca ttaatgatat tcgtacaaaa ataaaacccg
    15421 atttagaggt ctttggcgaa tattggaaat ctgaccaaac atcaatgaag gactatttag
    15481 aggcaacaca atttcaattt tctcttgttg atgtcacctt gcatatgaat ttttttgatg
    15541 ccagtcacca aaaccgtgat tttgatatgc gaacaatttt tgatgatagt cttgttattg
    15601 ataatcctga gtatgctgtt acattcgtag aaaatcacga tacgcaaagt ggacaagctc
    15661 tagaaagtag agtggaagat tggtttaaac ctttagctta tggcttgata cttttacgcc
    15721 aacaagggac tccatgtctt ttttatggtg attactatgg cattcaagga gaatttggcc
    15781 aaccatcctt taaagaggtt attgataaaa tggctgagct acgccaaaac tatgtatttg
    15841 gcgaacaagt cgattacttt acgcattcaa attgtattgg ttggacttgt cttggggatg
    15901 aggaacataa ttcttgttta gctgtggtcc taacaaatgg tgatcaggga tggaaacata
    15961 tggaagtagg ggaaatttat gcaggcaaaa cttttgttga ttatttaggc aattgtgaac
    16021 aagaggttgt tataggagat gatggttggg gtgatttttt agttgcatct gcgtctatct
    16081 cggcttgggt ccctaaaata gaggaagcta actgataaat caggtgaaaa aaaaggtaaa
    16141 atgagatata ataagaccta tgaaagtttt actgtattta gaggctgaag aatacttgaa
    16201 aaagtcaggt atcggtcgtg ctatcaaaca tcaagagaaa gctcttcaaa ttgctgggat
    16261 agattatacc actaatccta cagatgactt tgaccttgtt catatgaata cctatggtat
    16321 tagaagttgg cttttgatga gtaaagctaa aaaaactggg aaaaaagtaa ttatgcatgg
    16381 ccattccact gaagaggact ttagaaattc ttttattggc tcaaatttag tatccccgct
    16441 ttttaaatgg tacctttgcc gtttttatca aaaagcagat gccattataa ccccaactga
    16501 ttattctaaa caactaataa aagcatatgg catcaagaaa ccaatttttg ttttatctaa
    16561 cgggattgat ctaagtcgct atcagaggtc agaaaagaaa gaagctgcat ttcgtcacta
    16621 ttttcactta agtaaggatg ataaagttgt tatgggggcc ggtctttatt tcatgcgtaa
    16681 gggaattgac caatttgttg aagtagctgc aaaaatgcct gatattcgtt ttatttggtt
    16741 tggagaaaca aataaatggg ttattcctcg aaaggttagg caaatcgtta ccaagcaaca
    16801 tccctcaaat gttacttttg ctggttacat aaaaggtgat gtttatgaag gtgccatgag
    16861 tgctagcgat gctttcttct ttccgagtcg tgaagagaca gaagggatag tagttttaga
    16921 ggctttagct agtcatcagc atgtggtttt aagagatatc cctgtgtatc acggttgggt
    16981 gaccgaagac agtgttgaat tagcgactga tgttgatggt tttgttgaaa agttggataa
    17041 agtcttatct ggtaagagtg ataagattaa agaaggttat cgtgtggctg agagtcgaag
    17101 tatcgaacga attgcccatg aattggcaag tgtttatcaa aaagtaatgg agttgtaaga
    17161 tgcgtatagg tctatttaca gatacatact ttccccaagt ttcgggagtt tcaacaagta
    17221 tccgaacttt aaaggaaggg ttggagaagg aaggacacga agtttatatt ttcacaacta
    17281 ccgaccgtaa tgtcaaacgt tttgaagacc ctacaattat tcgtttacca agtgtccctt
    17341 ttatttcttt tacagatcgt cgagtagttt atagaggttt aatttctgct tataggatag
    17401 ctaaagacta cgaacttgat attattcata ctcaaacaga attcagttta ggacttttag
    17461 gaaaattagt tgcaaaagct ttaagaatac ctgttgtgca tacttatcat actcaatatg
    17521 aggattacgt tggttatatc gcaaaaggca aattgattaa gccaagtatg gtcaaatata
    17581 tcatgcgtac ctacctaagt gatttggatg gtgtcatatg tccttctcga attgttctca
    17641 atcttctaga tggttacggt gtcaaaattc caaaacaagt tattccgaca ggtattcctg
    17701 ttgagaacta tcgtcgagag gatatctctg aagaaacaat caagaatcta cgaacggagc
    17761 ttggccttgc ggataatgat acaatgctat tgagtttatc acgggtatct tttgaaaaaa
    17821 atattcaagc aatcttgatg catttatcag ctgtcgttga tgaaaatcct cacgtaaaat
    17881 tagtgatagt tggagatggt ccttatcttt cagatctaaa agaacttgtt cactctttag
    17941 aacttgaaaa ttcagttatt tttacaggta tggttgagca tagccaagtg gctatctact
    18001 ataaagcatg tgatttcttt attagtgctt caacaagtga aacgcaagga ttaacttaca
    18061 ttgagagtct ggcgagtgga cgtccaatca ttgcgcaaag caatccttat ctagatgatg
    18121 ttatttcaga taaaatgttt ggaactctgt acaagaagga aagtgttttg gcagatgcta
    18181 tcctagatgc aatagcagag acacccaaaa tgactcaaga agcttatgaa cagaaattat
    18241 atgagatttc tgctgagaat ttcagcaagt ctgtatatgc tttttaccta gactttctga
    18301 tttcacaaaa agctagtgtg aaagaaaaag tctctctgac gataggaaat aaggatagcc
    18361 attctactct tcgttttgtc agaaaagcgg tatatttacc taagaaagtt tttaccttta
    18421 caggaagagc ctctaaaaaa gtggtaaaag cacctaaacg tcgtatttca tctattcgag
    18481 attttttaga ttgagacgaa taaaattagt aaatgtcttg aaattataaa aaatattgtt
    18541 ataataaaaa atgaagacat gttaggttct aatagatttc aagagcgagc actggttggt
    18601 ggaaagagtg caattagaga acttagtact acttccttga ttttatgtaa acataaaagg
    18661 gtggttccct tatcgccact agaggtctga cacaagtata ctcgagtttt tgtgttggat
    18721 aaattgaggt ggaaccacgt agtctttacg tccttttgga tgtaaaggct ctttttttat
    18781 aaaagaagtg gttgcataaa agaggcagat tgcttttcta atgttatact tattatccct
    18841 atctaggata atgtggaatt caatcaaagg tcaatccctt tgtttattag tgaattaatg
    18901 tgtttaggaa ttggtatcac cctaacagtt attaagaaaa ggagaacaag atgattaaaa
    18961 tcactttccc agatggtgca attcgtgaat ttgaatctgg catcacaaca tttgaaattg
    19021 cacaatcaat ttctaactct ttagctaaaa aagctttagc tggtaaattt aatggtcaac
    19081 ttattgatac aacacgtgct atcgaagaag atggtagtat tgagattgta acacctgacc
    19141 acgaagatgc tttaggagtt cttcgtcact cagcagcgca tttatttgct caagctgcaa
    19201 aacgcctttt tccaaactta tgtttaggtg taggtccagc tattcacgat ggtttttact
    19261 acgatactga taataaatca ggtcaaattt caaatgatga tcttcctcgt attgaagaag
    19321 aaatgaaaaa aattgttaag gaaaatcacc cttgtattcg tgaagaaatc agtaaagaag
    19381 aagcgttaga actttttaaa gatgaccctt ataaagttga attaattagt gaacatgctg
    19441 aagatggttt aactgtttat cgtcaaggtg aatttgtaga cctttgtcgt ggacctcatg
    19501 tcccatcaac aggacgtatt caagtctttc atcttctaaa tgtcgctggg gcttactggc
    19561 gtggtaacag tgataatgct atgatgcagc gtgtctatgg tactgcttgg tttgataaaa
    19621 aagatttgaa agcatatctt aaacgccgtg aagaagctaa ggaacgtgac caccgcaaat
    19681 taggtaaaga acttgattta tttatggtta atcctgaagt aggtcaagga ttgccattct
    19741 ggttgccaaa tggagcaact attcgccgcg aattggaacg ttacattgtt gataaggaga
    19801 ttgcttcagg ttatcaacac gtttacacac ctccgatggc atctgttgaa ttttacaaga
    19861 cttctgggca ctgggaccac tatcgtgaag atatgttccc aacaatggat atgggagatg
    19921 gggaggaatt cgttcttcgt ccaatgaact gcccacacca tattgaagtt tataaacatc
    19981 atgttcactc atatcgtgaa ttgccaattc gtatcgctga acttggtatg atgcaccgtt
    20041 atgaaaaatc aggggccctc acaggattgc aacgtgtacg cgaaatgaca ctcaacgatg
    20101 cccatatttt cgttacacct gagcaaatta aagatgaatt tcttaaagct cttaatctca
    20161 tagcagaaat ttatgaagat tttaacttaa cagattaccg tttccgctta tcttatcgtg
    20221 acccagaaga taaacataag tattatgata acgatgagat gtgggaaaat gcacaagcta
    20281 tgctaaaaga ggctatggat gattttggat tagattattt tgaagcagaa ggcgaagcag
    20341 ccttttacgg tcctaaattg gatatccagg tgaaaactgc tcttggtaat gaagaaacat
    20401 tatctactat ccaactagat ttccttcttc cagaacgctt tgatcttaaa tacatcggtg
    20461 cagatggtga agaacatcgt ccaattatga ttcaccgcgg tggtatttca acaatggagc
    20521 gatttacagc tattttaatt gaaacttaca aaggtgcctt tccaacttgg cttgctccac
    20581 aacaagtttc tgttattcca atttcaaatg aagcccacat tgattatgct tgggaagtgg
    20641 ctcgtgccct taaagatcgt ggtattcgtg ctgaagtgga tgaccgtaac gaaaagatgc
    20701 aatataagat tcgtgcggcg caaacccaaa aaattccata tcaattgatt gttggtgata
    20761 aggaaatgga agaaaaagcc gtaaatgtgc gtcgctatgg aagtaaagcg actgaaacaa
    20821 aatctataga ggaatttgta gaaagtattt tagctgatat tgcaagaaaa tctagacctg
    20881 a
//
LOCUS       DBJYSH010000023        20259 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.023, whole genome shotgun sequence.
ACCESSION   DBJYSH010000023 DBJYSH010000000
VERSION     DBJYSH010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 20259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20259
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.023"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            169..1098
                     /locus_tag="ACUBV3_000683"
     CDS             169..1098
                     /locus_tag="ACUBV3_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001086432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HHU3847208.1"
                     /translation="MNSLDNLLSHNLEDNRHLLPYEHMHTEVRNGYPDILFHWHPELE
                     ISYVHEGTARYHIDYDFFNSQSGDIILIRPNGMHSIHPIENKEHITDSIKFHLDLIGY
                     SIVDQVSLRYLQPLQTSSFKFIQCIKPSMTGYNDIKNCLFDIFNISKEENRHFELLLK
                     AKLNELLYLLYYHQYVIKKHTDDTYRKNERIRDLIDYINNNYQQNLTIEFLADYMGYS
                     KTHFMTVFKQHTGTSCTEFIIQVRLNKASEHLINSTTAIIDIANSVGFNNLSNFNRQF
                     KRYYHTTPRQYRKQFTNKKDGSTLVYRNINTKK"
     gene            complement(1155..2687)
                     /locus_tag="ACUBV3_000684"
     CDS             complement(1155..2687)
                     /locus_tag="ACUBV3_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HHU3847209.1"
                     /translation="MENHKNEFQFSLESILGFVWRGIVVGLIAGFVVSIFRLAIEKIF
                     LVVMELYKSAHYQPIILLSITVTSIIAAVIIGFFIKSDPDIKGSGIPHVEGELKGMLS
                     PDWFSIVWKKFIAGILAISSGLMLGREGPSIQLGAMTGKGIAQYLNASRMEKRVLIAS
                     GAAAGLSAAFNAPIAGLLFVVEEIYHHFSRLVWITALVASLVANFVSLNIFGLTPVLA
                     LPSELPSLNLNFYWIFLLMGLFLGILGFIYEWVILRFHVIYDYLGKLFHLPSHLYGIL
                     AVIFILPIGYYFPQLLGGGNGLIVSLPRSNLSLMMLGLFFLIRFLWSMLSYSSGLPGG
                     IFLPILALGSLAGAFFAVGMQYFGIISHQQISLFVVLGMAGYFGAISKAPLTAMILVT
                     EMVGDLKQLMAIGIVTMVSYIVMDLLKGEPIYEAMLAKMTFNPKDKVMTPTLIELTVS
                     DKISGKYVRDLELPENVLITTQIHHKTSAVVSGNTILNAGDTIFLVVNESEIKEVREQ
                     LM"
     gene            complement(2796..3869)
                     /locus_tag="ACUBV3_000685"
     CDS             complement(2796..3869)
                     /locus_tag="ACUBV3_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HHU3847210.1"
                     /translation="MRRVYSFLGGIILVILILFGLTTYLEKKSSSTPNSDKLVIYNWG
                     DYIDPALLKKFTKETGIEVQYETFDSNEAMHTKIKQGGTTYDIAVPSDYMIDKMIKEN
                     LLVKLDHSKIANWDAIGARFKNLSFDPKNKYSIPYFWGTVGIVYNDQLVKTPPKHWDD
                     LWRPEFRNKIMLVDSAREVIGVGLNSLGYGLNTKNISELKAASKKLDALTPNVKAIVA
                     DEMKGYMIQGDAAIGVTFSGEAREMLDGNKHLHYVVPSEGSNLWFDNIVIPKTVKHRK
                     EAYAFINFMMEPKNAAQNAEYIGYATPNLKAKALLPADIKNDKAFYPPDKTIDHLEVY
                     NNLGQKWLGIYNDLYLQFKMYRK"
     gene            complement(3862..4638)
                     /locus_tag="ACUBV3_000686"
     CDS             complement(3862..4638)
                     /locus_tag="ACUBV3_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HHU3847211.1"
                     /translation="MKKFANIYLAFVFIILYIPIIYLIFYSFNKGGDMNSFTGFTFSH
                     YGELFQDSRLMLILVQTFFLAFLSALLATIIGTFGAIWIYQVRRRHQTSILSLNNILL
                     VAPDVMIGASFLLVFTVIGLQLGFTSVLLSHVAFSIPIVVLMVLPRLKEMNDDMINAS
                     YDLGASTWQMLKEVMLPYLSSGIISGFFMAFTYSLDDFAVTFFVTGNGFSTLSVEIYS
                     RARRGISLEINALSTIVFLFSILLVIGYYYISKEKGEKNA"
     gene            complement(4635..5429)
                     /locus_tag="ACUBV3_000687"
     CDS             complement(4635..5429)
                     /locus_tag="ACUBV3_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HHU3847212.1"
                     /translation="MKKTSSLFSIPYMAWLFLFVLAPVALIAWNSFFDINGHFTLANY
                     QTFFSSGTYLKMSFNSVLYAGIVSFITLLISYPAAYLLTKLKHKQLWLMLIILPTWIN
                     LLLKAYAFMGIFGQQGGINAFLTFIGIGPKQILFTDFSFLFVAAYIELPFMLLPIFNA
                     LDDIDQNLIYASDDLGANAWQTFQKVIFPLSLNGVRAGVQSVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLITQNKGMGSTIGVILILVMVAIMWLTKERKA"
     gene            complement(5413..6567)
                     /locus_tag="ACUBV3_000688"
     CDS             complement(5413..6567)
                     /locus_tag="ACUBV3_000688"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HHU3847213.1"
                     /translation="MTNPIIAFKNVSKVFEDSNTVVLKDINFELEEGKFYTLLGASGS
                     GKSTILNIIAGLLEASTGDIYLDGKRINDVPTNKRDVHTVFQNYALFPHMTVFENVAF
                     PLKLKKMDKKEIQKRVQETLKMVRLEGFEKRAIQKLSGGQRQRVAIARAIINQPKVVL
                     LDEPLSALDLKLRTEMQYELRELQQRLGITFVFVTHDQEEALAMSDWIFVMNEGEIVQ
                     SGTPVDIYDEPINHFVATFIGESNILSGKMIEDYLVEFNGKRFEAVDGGMRPNESVQV
                     VIRPEDLQITLPDEGKLQVKVDTQLFRGVHYEIIAYDDLGNEWMIHSTRKAIEGEVIG
                     LDFTPEDIHIMRLNETEEEFDARIEEYVDTDDHEDGLINAIEEERNEENL"
     gene            complement(6616..7518)
                     /gene="murB"
                     /locus_tag="ACUBV3_000689"
     CDS             complement(6616..7518)
                     /gene="murB"
                     /locus_tag="ACUBV3_000689"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HHU3847214.1"
                     /translation="MIKTIQKELEGLDIRFDEPLKKYTYTKVGGPADYLAFPRNRLEL
                     SRIVKFANSQNIPWMVLGNASNIIVRDGGIRGFVIMFDKLSTVTVNGYVIEAEAGANL
                     IETTRIARYHSLTGFEFACGIPGSVGGAVFMNAGAYGGEIAHILLSAQVLTPQGELKT
                     IEARNMQFGYRHSVIQESGDIVISAKFALKPGDHLMITQEMDRLTYLRELKQPLEYPS
                     CGSVFKRPPGHFAGQLISEAHLKGQRIGGVEVSQKHAGFMVNIAEGSAQDYENLIEHV
                     INTVESTSGVHLEPEVRIIGESLL"
     gene            complement(7662..8150)
                     /gene="folK"
                     /locus_tag="ACUBV3_000690"
     CDS             complement(7662..8150)
                     /gene="folK"
                     /locus_tag="ACUBV3_000690"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HHU3847215.1"
                     /translation="MTTVYLSLGSNIGDRETFLKQALFSIDHLQKTKVAQISAIYETA
                     AWGNTNQEDFFNICCQVETDLAPFELLDYCQEIEKRLKRVRHEHWGPRTIDIDILLFG
                     NQVINQEDLVVPHPYMTKRAFVLVPLLEIAPQLSLPNGSKLEDYLEKLNLGEVHYLKP
                     VE"
     gene            complement(8147..8509)
                     /gene="folB"
                     /locus_tag="ACUBV3_000691"
     CDS             complement(8147..8509)
                     /gene="folB"
                     /locus_tag="ACUBV3_000691"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HHU3847216.1"
                     /translation="MDKIYLNKCRFYGYHGAFSEEQTLGQVFQVDAVLSLDLAKASQT
                     DDLIDTVHYGEVFDCIKNHVENEQYQLIEKLAGVIVEDIFLQFHPVQAITLKITKDNP
                     PINGHYESVGIELERKRV"
     gene            complement(8511..9314)
                     /gene="folP"
                     /locus_tag="ACUBV3_000692"
     CDS             complement(8511..9314)
                     /gene="folP"
                     /locus_tag="ACUBV3_000692"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HHU3847217.1"
                     /translation="MKIGQYDITGKACIMGILNVTPDSFSDGGSYTTIDSALNQVGEM
                     LEQGVAIVDIGGESTRPGAVFVTAEEEIKRVVPMIKAIREVYPDLLLSIDTYKTEVAQ
                     AALDAGVHILNDVWSGLYDGKMLSLAAERNVPIILMHNQEEAVYQDIKKEVCEFLLER
                     AERALEAGVSKDNIWIDPGFGFAKTEEQNLELLKGLEQVCDLGYPVLFGISRKRTVNY
                     LLGGNREVTERDMGTAALSAWAIAKGCQIVRVHNVEVNKDIVTVISQLV"
     gene            complement(9318..9881)
                     /gene="folE"
                     /locus_tag="ACUBV3_000693"
     CDS             complement(9318..9881)
                     /gene="folE"
                     /locus_tag="ACUBV3_000693"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001133588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HHU3847218.1"
                     /translation="MPNQEKMEKAIYQFLEALGENPDREGLKDTPKRVAKMYIEMFSG
                     LNQDPKEQFTAVFSENHEEVVIVKDIPFYSMCEHHLVPFYGKAHIAYLPNDGRVTGLS
                     KLARAVEVASKRPQLQERLTAQVAQALEDALAPKGIFVMIEAEHMCMTMRGIKKPGSK
                     TITTVARGLYKDDRYERQEILSLIQKG"
     gene            complement(9900..11162)
                     /locus_tag="ACUBV3_000694"
     CDS             complement(9900..11162)
                     /locus_tag="ACUBV3_000694"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HHU3847219.1"
                     /translation="MTYQEALEWIHSKLAFGIKPGLERMRWMLEQLGNPQNNLSAIHV
                     VGTNGKGSTTSYLQHIFTNSGYQVGTFTSPYIVDFRERISIDGQMIPESDFIKLVETV
                     RPVVERLHLETNLEPATEFEVITVLMFYYFGNSCPVDIVIIEAGMGGYYDSTNMFKAL
                     AVTCPSIGLDHQEVLGRTYVDIAEQKVGVLKKGVPFVYANDRQDVEEVFQIKAKETHS
                     QTYRLHNDFYIKEEENYFNYIGPQANIDHIQLQMPGHHQVSNASIAITTSLLLRDKYP
                     KLTLQTIKDGLEMTKWVGRTELIFPNVMIDGAHNNESVDALVQVIKKYQQKNVHILFA
                     AINTKPIESMLESLSSIAPVSVTSFDYPKSINLDKYPKAYTRVSDWKKWLHDINLTSD
                     KDFYVITGSLYFISQVRQELLLIKTTTT"
     gene            complement(11164..12051)
                     /gene="rarD"
                     /locus_tag="ACUBV3_000695"
     CDS             complement(11164..12051)
                     /gene="rarD"
                     /locus_tag="ACUBV3_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HHU3847220.1"
                     /translation="MRKDNLGIILGLSAYVLWGLLSLYWKLLSGIEAYSTFAYRIIFT
                     VLTMLIYMLVSGRKTVYLKDLKGLVNNKKSFWTMFVASILISINWLVYIFAVTHGHAT
                     EASLGYYMMPIISILLSVLVLREHLARVVSLAILIAIMGVGILVYQTGHFPLISLTLA
                     LSFGFYGLLKKSISLSSDFSMLVESSFIAPFALIYIVFFAKDFLTDYNILQLVLLSLS
                     GIITAVPLLLFAEAIKRAPLNIIGFIQYINPTIQLLLALFIFKETIVSGEVIGFIFIW
                     LAILVFSIGQVHTMLKKGK"
     gene            complement(12038..12904)
                     /gene="thrB"
                     /locus_tag="ACUBV3_000696"
     CDS             complement(12038..12904)
                     /gene="thrB"
                     /locus_tag="ACUBV3_000696"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HHU3847221.1"
                     /translation="MRIIVPATSANIGPGFDSIGVALSKYLSIEVLEESTEWLVEHNL
                     VNIPKDHTNLLIQTALHVKSDLAPHRLKMFSDIPLARGLGSSSSVIVAGIELANQLGN
                     LALSQKEKLEIATRLEGHPDNVAPAIFGDLVISSIVKNDIKSLEVMFPDSSFIAFIPN
                     YELKTSDSRNVLPQKLSYEDAVASSSVANVMVASLLKGDLVTAGWAIERDLFHERYRQ
                     PLVKEFGVIKQISTQNGAYATYLSGAGPTVMVLCSKEKEQPIVTELSKLCLDGQIQVL
                     NIERKGVRVEKR"
     gene            complement(12906..14189)
                     /locus_tag="ACUBV3_000697"
     CDS             complement(12906..14189)
                     /locus_tag="ACUBV3_000697"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HHU3847222.1"
                     /translation="MTIKIALLGFGTVAKGIPYLLKENQHKLLSLEGEDIVIDKVLVR
                     DNESRQRFINQGFTYNFVTEINTILQDPQIDIVVELMGGIEPAKTYLSQALGFGKHIV
                     TANKDLIALHGKELMDLADARGLALFYEGAVAGGIPILRTLSHSFASDKMTRLLGILN
                     GTSNFMLTKMFEEGWSYEQALKKAQELGYAESDPTNDVEGIDTAYKATILSQFGFGMP
                     IDFDDVNYKGISSIRSEDVEVAQEMGFAIKLVTDLRETPTGISVDVSPTLISQEHPLA
                     AVNHVMNAVFIESIGIGQSLFYGPGAGQNPTATSVLADIIDISRSIRSQIKIKPMNTY
                     HCPCRLSMQSDIFNEYYLAISLRNAEDSDTLGRYFEQENIGLKNVIEKALDDKQQEIY
                     VLTDEVSQEKITQFIEEFPESGVIQLINVFKVIGG"
     gene            14366..15253
                     /locus_tag="ACUBV3_000698"
                     /pseudo
     CDS             14366..15253
                     /locus_tag="ACUBV3_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645955.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
     gene            complement(15276..16823)
                     /locus_tag="ACUBV3_000699"
                     /pseudo
     CDS             complement(15276..16823)
                     /locus_tag="ACUBV3_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037479.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
     gene            complement(16966..18342)
                     /locus_tag="ACUBV3_000700"
     CDS             complement(16966..18342)
                     /locus_tag="ACUBV3_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HHU3847223.1"
                     /translation="MAYKTIYPYTNEVLHEFDNISDSDLEQSLDIAHALYKTWRKEDN
                     VEERQNQLHKVADLLRKDRDKYAEVMTKDMGKLFTEAQGEVDLCADIADYYADNGQKF
                     LKPVPLESPNGEAYYLKQAVGVLLAVEPWNFPFYQIMRVFAPNFIVGNTMLLKHASIC
                     PASAQAFEDLVREAGAPEGAFKNIFASYDQVSNLISDPRVAGVCLTGSERGGASIAAE
                     AGKNLKKSSMELGGNDAFLILDDADFDLLSKTIFFARLYNAGQVCTSSKRFIVMADKY
                     DEFVNMVVETFKSAKWGDPMDSETTLAPLSSAGAKDDVLKQIKLAVDHGAEVVFGNDT
                     IDHPGNFVMPTVLTNITKANPIYNQEIFGPVASIYKVDTEEEAIALANDSSYGLGSTV
                     FSSDPEHAKKVAAQIETGMTFINSGWTSLPELPFGGIKNSGYGRELSQLGFDTFVNEH
                     LVFTPNSD"
     gene            complement(18416..19423)
                     /locus_tag="ACUBV3_000701"
     CDS             complement(18416..19423)
                     /locus_tag="ACUBV3_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HHU3847224.1"
                     /translation="MLFKEKIPGLILCFIIAIPSWLLGLYLPLIGAPVFAILIGIIVG
                     SFYQNRQLFNKGIAFTSKYILQTAVVLLGFGLNLMQVMKVGISSLPIIIMTISISLII
                     AYVLQKLFKLDKTIATLIGVGSSICGGSAIAATAPVINAKDDEVAQAISVIFLFNILA
                     ALIFPTLGNFIGLSDHGFALFAGTAVNDTSSVTATATAWDAINHSNTLGGATIVKLTR
                     TLAIIPITIVLSIYHMKQTQKEQSVSVTKIFPKFVLYFILASLLTTIVASLGFSLRIF
                     EPLKVLSKFFIVMAMGAIGINTNVSKLIKTGGKSILLGAACWLGIIIVSLTMQAILGT
                     W"
     gene            complement(19531..20217)
                     /locus_tag="ACUBV3_000702"
     CDS             complement(19531..20217)
                     /locus_tag="ACUBV3_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3847225.1"
                     /translation="MKKFLKVFLTVLVLILIVGAGGLYFWNNRQSLEGKWRTVSLEKQ
                     VEKEIEQQLGSQAADMGISAADLVKGANMHMNVKNDEAKITVTAQIDEVKFHQAIKTF
                     IDKALEKQLKDQGLTYNDLSEAGKKIFDETKITDKQIDQQIDRSFQSAAQAVGGKYNT
                     NTGEMTLPVMDGKVHRLTSVIKVSHINKKANAFYGNIVKNGEKTAYKKEGSKLILGNE
                     KSYPFMKVTK"
BASE COUNT         6875 a         4065 c         3022 g         6297 t
ORIGIN      
        1 actctaagca aaaattttaa agcgttttct ctatgactta tattatatgt catgttccta
       61 tacaaaactt acactatcct ttcaaaaaat aatactatcc tacgaaaccc attttttgat
      121 agttttctgt tataataata ctatcctatg attttagaaa gaaaaacgat gaactcactt
      181 gataatttac tatcacataa tttagaagat aaccggcatt tattacccta tgaacacatg
      241 catactgagg ttagaaatgg ttaccctgac attttatttc actggcatcc ggaacttgaa
      301 atttcatatg tccacgaggg gaccgcacgt tatcacatcg attatgattt ttttaatagc
      361 caatctggtg atattatctt gatccgtcca aatggtatgc attctattca tccgattgaa
      421 aataaagagc atatcacaga tagcattaaa tttcacttag atttaatagg atattctatt
      481 gtagaccaag ttagccttcg ttatttacaa ccgttgcaga ctagctcttt taagtttatt
      541 caatgtatca aacctagtat gacaggatat aatgatatca aaaattgttt gtttgatatt
      601 tttaatattt caaaagaaga aaatcgacat tttgagctac tcctaaaagc taaattaaac
      661 gaactcttgt atttactcta ttatcatcaa tatgtcatta aaaaacatac tgatgatacc
      721 tacagaaaaa acgagagaat tcgtgatctg attgattata ttaataataa ttaccagcaa
      781 aatcttacca ttgaattttt agctgattat atggggtata gtaagacaca ttttatgacc
      841 gtatttaaac agcatactgg aacatcctgt acagaattta tcatacaagt tcgtcttaat
      901 aaagctagtg agcatcttat taactccact acggccatta ttgatattgc caacagcgtt
      961 ggttttaata atctatcaaa ttttaatcgc caatttaaac gctattatca tacaactcct
     1021 cgccagtacc gcaaacaatt taccaacaaa aaagacggta gtacgcttgt ctatagaaat
     1081 ataaatacaa aaaaatagaa actagttaaa tccatgacta gtttctattt tttattattt
     1141 aagcgttaat tagtttacat cagctgctca cgtacttctt taatttccga ttcattcaca
     1201 actaaaaaaa tggtgtcgcc agcattcaag atagtatttc ctgatactac agccgatgtc
     1261 ttatgatgaa tttgagtcgt tattagcaca ttttctggta attctaaatc tcggacatac
     1321 tttcctgata ttttatctga aacagttaat tcaatcaatg taggtgtcat tactttatct
     1381 tttggattga aggtcatttt agccaacatc gcttcataaa taggttctcc ctttaataaa
     1441 tccatgacaa tataagatac cattgttaca attccaatag ccattaattg ttttaaatcg
     1501 ccaaccattt ctgtaaccaa aatcatagct gttagtggtg cttttgagat tgcaccaaaa
     1561 tagcccgcca ttcctagaac aacaaataaa gatatctgct gatgagatat tataccaaag
     1621 tactgcatac ctactgcaaa aaaagcacct gctaaagatc ctaaagctag aattggtaaa
     1681 aaaattcctc ctggcaagcc actactgtaa ctcaacatac tccataaaaa acgaatgagg
     1741 aaaaataatc ctaacatcat cagactaaga ttactcctag gcagtgatac aattaagcca
     1801 tttccacccc ccaataattg tgggaaatag taaccaatag gaagaataaa aattaccgct
     1861 aatataccat ataggtgaga tggtagatga aatagtttac ctaaataatc atagatcaca
     1921 tgaaatctca aaattaccca ctcatagata aagcctaaga tacccaaaaa gagacccatg
     1981 agtaaaaaaa tccaataaaa gttgagattt aaagatggta attctgatgg taaagctaaa
     2041 actggggtta aaccaaagat attaagtgag acaaagtttg ccactaaact tgctaccaaa
     2101 gctgttatcc aaactaagcg agaaaaatga tggtaaattt cttcaactac aaataaaagg
     2161 cctgcaatag gagcattgaa ggctgctgaa agacctgctg ctgcaccact agcaattaaa
     2221 acacgttttt ccattcgact agcattgaga tactgtgcaa ttcccttacc tgtcatagct
     2281 cctaactgaa tactgggacc ttcgcgacca agcataagtc ccgaagaaat agctaaaata
     2341 cccgcaatga atttcttcca tacaatacta aaccagtctg gcgatagcat tcctttaagt
     2401 tcaccttcaa catgcgggat acctgaacct ttaatatcag gatcagattt gataaaaaaa
     2461 cctattatca ctgcagcgat aatactagta accgtaatgc tcaataaaat aattggttga
     2521 taatgggcac ttttataaag ttccataaca actaaaaaaa ttttttcaat cgctaatcga
     2581 aatatgctga caacaaatcc tgctattaag cctactacta ttcctctcca aacaaatccc
     2641 agaattgatt caagtgaaaa ctgaaattca tttttatgat tttccatatt ttcctccttt
     2701 tcttacaatt attttaacaa aaatatcaca aactttctag gaactcttgg taactaaaaa
     2761 tacctctcat cagagaagta cttttagtta atagattact tacgatacat tttaaattgt
     2821 aagtacaaat cattataaat accaagccat ttttgaccca agttattata aacttctaaa
     2881 tgatcaattg ttttatcagg aggataaaat gctttatcat ttttgatatc tgctggtaac
     2941 agcgcttttg ctttgagatt aggggtcgca taaccaatat attctgcatt ttgtgccgca
     3001 tttttaggtt ccatcatgaa attaataaag gcataagctt ctttgcgatg ctttactgtt
     3061 ttggggatta caatattatc aaaccaaagg ttcgatcctt cactaggaac aacgtaatgc
     3121 aaatgcttgt tcccatcaag catttctcgt gcttctccag agaatgtaac accaatcgct
     3181 gcatctccct gaatcatata gcctttcatc tcatcagcaa caatagcctt aacgtttggt
     3241 gttagagcat ctaacttttt agaagcggct tttaattctg atatgttctt tgtattcaat
     3301 ccatatccca aggaatttaa accaactcca ataacctcac gtgcactatc aactagcatg
     3361 attttatttc gaaactctgg acgccataaa tcatcccaat gtttaggagg agttttaact
     3421 aattgatcat tataaacaat cccaacagtt ccccaaaaat aaggaatcga atatttattt
     3481 ttaggatcga aactcaaatt tttaaaccta gctccaatag catcccaatt agctatcttt
     3541 gaatgatcaa gtttaaccag aaggttttct tttatcattt tgtcaatcat ataatcacta
     3601 ggtacagcaa tatcatatgt cgtcccaccc tgcttaattt tggtgtgcat agcctcatta
     3661 gagtcaaacg tttcgtattg tacttcaatt ccagtttctt ttgtaaattt ttttaataag
     3721 gctggatcaa tatagtctcc ccagttataa ataactaact tatctgagtt aggtgttgaa
     3781 ctactcttct tttctaagta agtggttaaa ccaaagagga tcaggataac aagaataata
     3841 ccgcctaaaa aggaataaac tctacgcatt cttttctccc ttctctttcg aaatatagta
     3901 ataaccaatt accaataaaa tactaaataa aaagacaata gttgacaagg cattaatttc
     3961 caatgatata ccgcgacgtg cgcgtgaata gatttctact gaaagcgttg aaaagccatt
     4021 acctgtaaca aagaaagtaa ccgcaaaatc atctagcgag taagtgaaag ccataaagaa
     4081 acctgaaata atacccgacg acaaataggg taacataact tctttaagca tttgccaagt
     4141 tgaagctcct aaatcatagg aagcattaat catatcatca ttcatttctt ttaaacgagg
     4201 taagaccatt aaaacaacaa taggaataga gaatgcaaca tggctcaata atacagaagt
     4261 gaaacctaac tgtaaaccaa taacagtaaa tactaagaga aaacttgcac caatcataac
     4321 atctggtgct actaaaagga tattattaag cgataaaata gaggtttgat gtctacgtct
     4381 tacctgataa atccaaatag ctccaaaggt accaataatt gtagctaata aggcgctaag
     4441 aaaagccaaa aagaatgttt gcactaaaat tagcattaag cggctatcct gaaacagttc
     4501 tccatagtga ctaaaggtga atccagtaaa agaattcata tccccacctt tattgaaaga
     4561 ataaaaaatc aaataaataa taggaatata aaggataata aaaacaaatg ctaaatagat
     4621 atttgcgaat tttttcatgc tttcctctcc tttgttaacc acataatagc aaccatcact
     4681 aaaattaaaa taacccctat ggttgagccc atacctttat tttgtgtgat taaaaaatgt
     4741 tgttctatgg ctgtccctaa agtaataact cgattaccac caataagacg cgtaagcata
     4801 aagagactta aactaggaat aaagacagat tgtacacctg ctcttacacc attcagagat
     4861 agaggaaata taaccttctg aaaagtttgc catgcatttg cacccaagtc atcacttgca
     4921 taaatgagat tttgatcaat atcatccaag gcattaaaaa tgggtaataa cataaacggt
     4981 aattcaatgt aagctgctac aaaaagaaat gaaaaatctg taaatagaat ctgttttggg
     5041 ccgataccta taaaggttaa gaaggcgtta attccccctt gttgaccaaa tatccccata
     5101 aaagcatagg cttttaacaa aagattaatc catgttggca aaataattag cattagccat
     5161 aattgcttgt gttttaattt tgtaagcaaa taagcagctg gatagctaat caaaagcgtt
     5221 atgaaactaa cgatgcctgc atataggacc gagttaaagc tcattttaag ataggttcct
     5281 gagctaaaaa acgtctggta attagctaaa gtaaagtgcc cattaatatc aaagaacgag
     5341 ttccaagcaa tcaaagcaac aggtgctaat acaaataaga ataaccaagc catataagga
     5401 attgaaaata gactagaggt tttcttcatt tctctcctcc tcgatagcat tgattaaacc
     5461 atcttcatga tcgtcagtgt ctacatattc ttcaatgcgc gcatcaaatt cttcttctgt
     5521 ttcattaaga cgcataatat gtatatcttc tggagtgaaa tctaacccaa taacttcacc
     5581 ctcaatagct ttacgagttg aatgaatcat ccattcatta cctaaatcat cataagcaat
     5641 aatttcatag tggacacctc gaaaaagttg tgtgtccact ttaacttgaa gttttccttc
     5701 atcgggtagg gtaatttgaa gatcttctgg acgaataact acttggactg attcattagg
     5761 gcgcataccg ccatcaacag cctcaaagcg cttaccgtta aattcaacca aataatcctc
     5821 aatcatcttg ccagataaga tatttgattc acctataaaa gttgctacaa aatgattgat
     5881 aggttcatcg tagatatcta ctggtgttcc tgattgaaca atttcacctt cattcatgac
     5941 gaaaatccaa tcactcattg ccaacgcttc ttcttgatca tgtgtgacaa aaacaaaggt
     6001 aattcctaaa cgttgttgca gttcgcgcaa ttcatattgc atctctgtac gcaatttcaa
     6061 atccaatgct gataagggtt catctagtaa gactacttta ggttgattga tgatagcacg
     6121 cgctatcgca acacgctggc gttgtcctcc tgataatttt tgaatagctc gcttttcaaa
     6181 cccttctaaa cgaaccattt ttagagtttc ttgaacacgt ttttgaattt ctttcttatc
     6241 catttttttc aactttaatg gaaaagcaac gttttcaaaa accgtcatat gtggaaacaa
     6301 agcatagttt tggaaaacgg tatgaacatc ccgtttatta gtgggaacat cattgatgcg
     6361 tttcccatct aagtaaatat cgcctgtact cgcctctagc aaacctgcaa taatatttaa
     6421 gatagttgat ttaccacttc ctgaggcccc taataaggtg taaaatttac cttcttcaag
     6481 ttcaaaatta atatctttta agacaaccgt gttgctgtct tcgaatacct ttgatacatt
     6541 tttaaatgca atgattgggt tagtcaattc tcataaatcc tccatttttc tgactaaagt
     6601 aagtactaaa ctactttata atagcgactc accgataata cgaacttctg gttccaaatg
     6661 gacacctgaa gttgattcaa cggtgttaat aacatgttca ataagatttt cataatcttg
     6721 agctgaacct tctgcaatgt taaccataaa accagcatgt ttttgactaa cctctactcc
     6781 accaatacgt tgacctttaa gatgtgcttc gctaatgagt tgccctgcaa aatgtcctgg
     6841 aggacgtttg aacacagaac cacaagaagg gtattctaaa ggttgtttca gctcacggag
     6901 ataggttaaa cgatccattt cctgagtaat catgagatga tctcctggtt ttaaggcaaa
     6961 cttagctgat ataacaatat ctccactctc ttgaatgaca gaatgacgat aaccaaattg
     7021 catattccta gcttcaatcg ttttgagttc accctgtggt gtcaaaactt gtgcagacaa
     7081 taaaatatga gcaatttcac caccgtaagc gccagcattc ataaagactg ctccacctac
     7141 acttccagga attccgcaag caaactcaaa gccagttaaa ctatgataac gtgcaatacg
     7201 agttgtttca atgagattag ctcctgcttc tgcttcaata acataaccgt tgactgtaac
     7261 agttgacagt ttatcaaaca tgataacaaa accacggata ccaccatctc taacgataat
     7321 attactcgca tttcctaaaa ccatccaagg aatattttga ctgttagcaa acttaacaat
     7381 acgcgatagt tcaaggcgat tccttgggaa tgcaagataa tcagcaggtc ctcctacttt
     7441 agtataagta tatttcttta aaggctcatc aaagcgaata tcgagacctt ctaattcttt
     7501 ttgtattgtt ttaatcataa cctttatctc aattctgttt tattgtgttt tatagacaca
     7561 aaaaatcggc ataaaaccga tttctattat accaaaaata taggttgttg acgatacatt
     7621 atcacaattt ctgtgtattt taattttaaa atcctaactg actattcgac tggttttaaa
     7681 taatgaactt ctcctaaatt caatttttcg agatagtctt ctagcttaga accattaggt
     7741 aaacttagtt gaggtgctat ttcgagtaac ggaactagaa caaatgctcg cttcgtcata
     7801 tacggatggg gtactactaa atcttcttgg ttaatcactt gattaccaaa aagtaagata
     7861 tcaatatcta ttgtccttgg accccagtgc tcatgtctca cgcgctttaa gcgtttttca
     7921 atttcttgac aataatccaa taattcaaaa ggtgctaagt ctgtctctac ctgacaacat
     7981 atattaaaaa aatcttcttg gttagtattc ccccaagcag ctgtttcata aatagcggaa
     8041 atttgcgcga cttttgtctt ttgcaaatga tcaattgaaa agagagcctg cttcagaaaa
     8101 gtttctctat caccaatatt actacctaaa ctcaagtaaa cggttgtcat actcgcttgc
     8161 gctccaattc aattccaact gattcatagt gcccattaat tggtggattg tcttttgtaa
     8221 tctttaatgt aatagcttgt actggatgaa actgtaagaa aatatcttca acaataacac
     8281 ctgctaattt ctctattaat tgatactgtt cgttttcaac atgattttta atacagtcaa
     8341 aaacttcacc ataatgaacc gtatcaatta aatcatcagt ttgtgatgct tttgctaaat
     8401 caagtgatag aacagcatca acttgaaaaa cttgccccaa tgtttgttct tccgaaaaag
     8461 caccatgata accataaaac cgacatttat ttaaataaat cttatccata ttacaccaac
     8521 tgactaatta ctgtcacaat gtccttattt acttcaacat tgtgaacacg tacaatctga
     8581 cacccctttg ctattgccca tgccgataaa gcagcagtcc ccatatcacg ttcagttact
     8641 tccctattac cacctagaag ataattaaca gtacgttttc gagagatccc aaataacaca
     8701 ggataaccta aatcacacac ttgttccaaa ccttttaata gctctaaatt ttgctcctct
     8761 gttttagcaa aaccaaagcc aggatctatc caaatattat cctttgaaac acctgcttct
     8821 aaagcacgct ctgctctttc aagtaagaat tcacaaactt cttttttgat atcttgataa
     8881 acagcttctt cctggttatg catcaaaata attggaacat ttcgttctgc tgctaaagat
     8941 agcattttac catcatataa acctgaccaa acgtcattta aaatatgtac acctgcatca
     9001 agggctgctt gagctacctc tgttttataa gtatctattg ataacaaaag atcagggtag
     9061 acctctctaa tagctttaat cattggaact acacgtttaa tttcttcttc agcagtcaca
     9121 aatactgcac ctggccgcgt tgattctccc ccaatatcta caattgccac cccttgttca
     9181 agcatctcac caacttgatt taaagctgaa tcaattgtgg tataagaacc accatctgag
     9241 aaggaatcag gcgttacatt taaaatcccc atgatacaag ctttacctgt aatatcatat
     9301 tgtcctattt tcatctttta tcctttttgt attagcgata gaatttcttg tctttcatat
     9361 cgatcgtcct tgtataaacc acgagctacc gttgtaatcg tcttacttcc tggtttttta
     9421 atgccacgca ttgtcatgca catatgctct gcttctatca taacaaaaat acctttaggc
     9481 gctaaagcat cctctaaagc ttgtgcaact tgtgctgtta aacgttcctg aagttgagga
     9541 cgcttactcg ccacttcaac tgcacgtgca agcttactaa gtccagtaac acgtccatcg
     9601 tttggtaaat aagcaatatg agctttacca taaaaaggaa ccaaatgatg ttcacacatg
     9661 gaatagaatg gaatatcctt gacaatcaca acctcttcat gattttcaga aaagactgca
     9721 gtgaattgct cctttggatc ttgattaagt cctgagaaca tttctatata catttttgcc
     9781 acacgcttcg gtgtatcttt caacccttct cgatcaggat tttctcctaa agcctctaaa
     9841 aattgataaa tagctttttc cattttttct tgattgggca ttaatttatc ctatcctttt
     9901 taagttgttg tagttttgat taatagtagt tcctgacgaa cttgagaaat aaaataaagg
     9961 gagccagtaa tcacatagaa atctttatct gatgtcagat ttatatcatg tagccatttc
    10021 ttccaatcac taactctagt gtacgcttta ggatatttgt ctaagtttat tgatttaggg
    10081 taatcaaagc tagtaaccga aaccggagcg atactagaca atgattctaa catactctct
    10141 atcggtttag tattaattgc agcaaataga atatgcacgt ttttctgctg atattttttt
    10201 ataacttgaa cgagagcatc gacactttca ttattatgag cgccatcaat cattacattt
    10261 ggaaagatta attctgtacg gcctacccat tttgtcattt caagcccatc tttaatggtc
    10321 tgtagggtta actttggata tttgtctctt aataataaac tggtagttat agctatgcta
    10381 gcattggaaa cttggtgatg tcccggcatt tgcaactgaa tatgatcaat atttgcttga
    10441 ggacctatat agttaaaata attttcctct tcttttatat aaaagtcatt gtgtaaacga
    10501 taagtctggc tgtgggtttc cttggctttt atttgaaaga cttcttcaac atcctgacga
    10561 tcattagcat atacgaatgg aactcctttt tttaaaacac ctactttttg ttcagctata
    10621 tctacataag ttcgtcctaa aacctcctga tgatctaatc caattgacgg acaagtaacc
    10681 gctaaagcct tgaacatgtt ggtagaatca taataaccgc ccattccagc ctcaataatg
    10741 acgatatcaa ctggacagct attgccaaaa taatagaaca tcaaaactgt tataacttca
    10801 aattctgtag ctggttctag gttggtctca aggtgcaacc gttcaacaac tggacgaaca
    10861 gtctcaacaa gtttgataaa atcagactct ggaatcattt gaccatcaat gctaatacgc
    10921 tctctaaagt caacaatata tggtgatgta aatgttccaa cttgataacc agaatttgta
    10981 aaaatatgtt gtaaataact agttgttgat cctttaccat tcgttccaac aacatgaatg
    11041 gcagataggt tgttttgagg gtttcctaac tgctctagca tccatctcat acgttccaaa
    11101 cctggtttaa taccaaaagc taatttgcta tgaatccatt ctagcgcttc ttgataagtc
    11161 atcttattta cctttcttta acatcgtatg tacttgtcca atactaaaaa ctaaaatagc
    11221 aagccagata aaaataaaac ctattacctc cccactaacg atagtttctt taaaaataaa
    11281 caatgctagc aataattgga tggttggatt gatgtattgg ataaaaccga taatatttag
    11341 aggagccctc ttaatggctt ctgcaaataa taaaagagga actgctgtaa tgattcctga
    11401 aagtgagagt aaaactaatt gtaatatatt ataatcagtt aaaaaatctt tggcgaaaaa
    11461 cactatataa atgagtgcaa acggagcaat aaaactactt tctaccagca tcgaaaaatc
    11521 gctagataaa gaaattgatt tttttaataa cccataaaaa ccaaaagaaa gcgctagagt
    11581 tagtgaaatt aaaggaaaat gaccagtttg ataaactaaa attccaactc ccatgattgc
    11641 aatcaaaatt gcaaggctaa ctactcttgc caggtgttca cgtaaaacaa ggaccgacaa
    11701 caaaattgaa ataatcggca tcatataata accaagactt gcctcagtag cgtgaccatg
    11761 cgtgactgca aaaatataga ccaaccaatt aattgaaatt aaaatactgg ctacaaacat
    11821 tgtccaaaat gactttttat tattaactaa gccttttaag tcttttaaat agactgtttt
    11881 tctacccgaa acgagcatat aaataagcat agttaaaaca gtgaatataa tcctataagc
    11941 aaacgtacta tatgcttcaa ttccagataa taatttccaa taaagagata aaagccccca
    12001 taaaacatag gctgacaagc caaggataat tcctaaatta tcttttctca actctcactc
    12061 cttttcgctc aatattaagt acttgaatct gtccatctaa acataattta cttaactctg
    12121 tgacaattgg ttgttctttt tctttcgaac acagaaccat taccgtcggt cctgctccag
    12181 acaaataagt agcatacgca ccgttttgag tgctgatttg ttttataact ccaaattcct
    12241 tcaccaaagg ttgacgatat ctttcatgaa agaggtccct ttctatagcc catccagcag
    12301 ttaccaaatc acctttcaac aacgacgcaa ccatcacatt agcgacgctg cttgaagcaa
    12361 ctgcatcctc ataactcaat ttttgaggta gtacatttcg actatcactt gtttttaatt
    12421 cataatttgg aataaaagcg ataaaggaac tatctggaaa catcacttcc aaacttttaa
    12481 tatcattttt cactattgat gaaataacta aatcaccaaa aatggccggt gctacattat
    12541 caggatgtcc ttctaatcgg gtagctatct ctaatttttc tttttgagaa agagccaaat
    12601 ttcctaattg attggctaat tctattcctg caacaattac agaggaagaa gaacctaaac
    12661 ctctggccaa aggaatatct gaaaacattt ttaagcggtg tggtgccaaa tccgacttaa
    12721 catgcaaagc tgtttgaata aggaggttag tatgatcttt tggaatattt actaaattgt
    12781 gttcaactaa ccactctgta ctttcttcta atacttcaat gcttaaatac tttgataacg
    12841 caactcctat tgaatcaaag cctggaccaa tattcgcact agttgcaggt acaattatcc
    12901 tcatttcacc ctcctattac tttgaaaaca ttgattaact gaatgacacc actctcagga
    12961 aactcctcaa taaattgagt tattttctct tggctaactt catctgttaa tacatagatt
    13021 tcttgttgtt tatcatccaa tgctttttca ataacatttt tcaaacctat attttcttgc
    13081 tcaaagtacc ttccaagtgt atcactatct tcagcatttc tcaaagaaat agctagatag
    13141 tactcattga agatatcaga ctgcattgac aacctacacg gacaatgata agtattcata
    13201 ggcttaattt ttatctgtga tcgaatacta cgactaatat cgatgatatc ggctaaaaca
    13261 gaggttgctg ttggattttg tcccgcacct ggtccataaa aaagagactg accaatccct
    13321 attgattcaa tgaatactgc attcatcaca tgattaactg cagctaaggg atgctcttga
    13381 gaaattagtg tcggagaaac gtctacactt ataccagttg gagtttcacg aagatcagtt
    13441 accaacttaa tggcaaagcc catctcctga gctacttcaa cgtcctctga gcgaatacta
    13501 gaaatcccct tataattaac atcatcaaaa tcaataggca taccaaatcc aaattgactt
    13561 aagatagtgg ctttgtaggc agtatcaata ccttcaacat catttgtagg atcactttcg
    13621 gcataaccta actcttgtgc cttttttaga gcttgttcat aagaccatcc ctcttcaaac
    13681 atttttgtta acatgaagtt ggaggtaccg ttgagaattc ctaataaacg tgtcatttta
    13741 tctgaggcaa acgaatgcga tagggtcctt aaaataggaa tgcctccagc aactgctccc
    13801 tcatagaata aagctagacc tctagcgtct gctaaatcca tcaactcttt tccgtgtaaa
    13861 gcaatgagat ctttattggc tgtcacaata tgtttaccaa atcctaatgc ttgactcaaa
    13921 taagttttag ctggctcaat cccccccatt aattccacta caatatcaat ttgtggatct
    13981 tgaagaatag tatttatctc tgtcacaaag ttataagtaa atccctgatt gatgaaacgc
    14041 tggcggcttt cattatctct tactaatact ttatcaatta caatatcttc gccttctaaa
    14101 gaaagtagct tatgttgatt ttcttttagc aaatatggaa tacccttagc aaccgttcca
    14161 aaacctaata aagctatttt aatagtcatg aatttctcct tagaaattgt ctctaacttc
    14221 aagacaaaca ttggctatat tataacaaat taccaatatt ttttataggg taaacgcgtc
    14281 ttgtcctatg aaagttctta aaaagagaat attatttcag aaaagatgat acaataaaag
    14341 tactttaaaa gttaaggaga aataaatggc acacacacct actagtcatc gtaaaccacg
    14401 aaaacgttcc ccatggttag ctattgcttc tgtctttttt ttgttaatag ctttaatcgg
    14461 catatttcta ttcttcaaca atcgttcaaa gcaagaaata aaaacaaaaa cgaacgcctc
    14521 tagtcatcga aaaattgtca catctatcaa aaaaaagaaa tgggttaagc aaaagactcc
    14581 tgttaaaata cccattttaa tgtaccatgc tgttcatgtt atggaccctt ctgaagccgc
    14641 cagtgctaac ttaattgtgg cacctgacat ttttgaaagt catataaaac gtctcaaaaa
    14701 ggaaggatat tacttcttag cccctaacga ggcatatcgt gcactaaacg aaaatgcttt
    14761 acctgaaaaa aaagtcattt ggattacttt tgatgatgga aatgccgatt tctacactaa
    14821 ggcataccct atactcaaaa agtacaaggt taaagccact aataatatca tcactggctt
    14881 tgttcaataa ggacgagaat ctaacctcaa cgttcaacaa atgctagaaa tgaagcaaaa
    14941 cggcatgtca ttccaaggac atacggttac tcaccccaat ctttctctct taactccaga
    15001 actacaaacc caagaaatga cactatcaaa actgttttta gatcaaaaac tctctcaaga
    15061 tactcttgcg attgcttatc catcaggacg ctacaatcct acaactttgg atattgctag
    15121 tcaatattat aaactagggc ttacaacaaa cgaaggagtt gcaacaaaag ataacggttt
    15181 gctttctctt aatcgagttc gtatcttacc aactactagc gatgatgatt tgatcaaaac
    15241 gattaaccaa taaaaagaag ccttctggct tctttttatt cttcataaga tttaaatggt
    15301 tcatttttac gtggataacg atctggagaa atcgataagc ccatttcttt tttctcatgg
    15361 aaaagctcca ctaatagcgc tagcatcatc aaaatcatta cgaatgaaaa tggcagagcg
    15421 acaataatta aaacgttctg cagcgctaac agaccaccag acaagagtaa gacaatagca
    15481 atcactgcca acactagccc ccaaattact ttagttcgat ttttgggatt taaattacca
    15541 tcttctgtca acatagccaa aacgtacgtt gcagaatctg ctgatgtaat aaaaaatgaa
    15601 aaaattaaaa tgatagcaat agtggataaa agccaaccta aagtgtagtg attaaaagta
    15661 gcaaacaata cttcctctgt tgcaaacttt gtcaaattaa tacccagttg ttgtacctga
    15721 gttgataatg tgccaaatgc tgcaaaccat acaaaactca ataatgtcgg tattaaaaga
    15781 actaccgtta aaaactcccg aatgctacgc cctttagaaa tacgagcgat gaaaactcca
    15841 acaaaaggag accaggaaat ccaccacgcc caataaaaaa tcgtccaatt atcaatccaa
    15901 gaacgtttag tattgtcaaa agcagctgca cgaaagctca ttccaaagaa attttgtaaa
    15961 taagagccta aagactctgt tagggtatca aaaatacgaa ccgttggtcc caatacaata
    16021 actaaagcta agagggctac cgctaaaatc aaatttaagt tcgataaaat tttaacacct
    16081 tttcctaaac ctgataaagc tgacataaca aataaagctg ttgtaataag gataataaca
    16141 atttgaacaa atgcattatt gggaacaccc aataaaaaac ttaaccctcc gttgatttga
    16201 gcagctccaa atccaagtgt cgtagcaact ccaataactg tagcaacaac tgtggtgata
    16261 tcaacaattt ttcctagtca accatctgtc ttatcaccga aaagaggctt taaagtgaca
    16321 gacaagaggt atttctcttg ctttcgaaat ccaaaataag ctagagccaa agcaaccaat
    16381 gcatatactg cccaagcatg tatcccccag tgaaaaaatg taaaacgaaa tgcatcagct
    16441 aatgctgttt gcgattcctt aggtgcgcca ggtgtcgaaa tagcaaaatg cgataagggg
    16501 tcagctgctc cgtagaagac caaaccaatc cccatacctg ctgaaaacat cattgctatc
    16561 catgatacag ttgaatattc aggacgtgaa tgaggtcgcc ctaaacgtat ttgtccaaga
    16621 ggactcataa taaaaaagac acacaaagca agaattgaag taactaacaa caagtacaac
    16681 caaccgaaat tggtagatac ctgttcacgc aagatttgtg tccaagattg aaaaccacga
    16741 ggaacaataa ttcctaataa gactaaaata agcgatacaa ttagtgaacc tgtaaacaca
    16801 ggcgtaatat gttttttact caaaatttcc tcctatacta aaatagtata gcatacttta
    16861 ataaatactc ctctcatttt aatcacctgt atcaagacct ctccttgtct cctcaacaaa
    16921 aaagaaccag ataacattat ctggttcttg tgtttgactt agtgattaat cactatttgg
    16981 tgtaaatacc aaatgttcgt tgacaaaggt atcaaatcca agttggctca attcacgacc
    17041 atatcctgaa tttttaatac ctccaaacgg taattccggt aatgatgtcc accctgaatt
    17101 aataaatgtc atccctgttt caatctgagc cgccactttt ttagcatgtt ctggatcaga
    17161 agagaaaaca gtacttccta aaccataact agaatcatta gctaaagcaa tagcttcttc
    17221 ttcagtatcc actttataga tagaggctac ggggccgaaa atttcttgat tatagattgg
    17281 atttgcttta gtgatattcg ttaaaacagt tggcatgacg aagtttcctg gatgatctat
    17341 agtgtcattt ccaaaaacta cctcagcacc gtggtctacc gccaatttaa tttgttttaa
    17401 aacatcatcc tttgcacctg cagatgacag tggagccaag gttgtttccg aatccattgg
    17461 gtcaccccat ttagcagact taaaggtctc aactaccata tttacaaatt cgtcatactt
    17521 atcagccata acaatgaaac gttttgaaga tgtacatact tgacctgcat tatataaacg
    17581 cgcaaagaat attgttttac taagtaaatc aaaatcagca tcatctaaaa taagaaaagc
    17641 atcgttccca cctaattcca tagacgattt tttcaaattt ttaccagcct cagctgctat
    17701 tgatgcccca ccacgctcag aaccagttaa acacacacct gcaacacgag gatctgatat
    17761 aaggtttgaa acttgatcat acgaagcaaa aatattttta aacgctcctt ctggagctcc
    17821 tgcttctcgg actaaatcct caaatgcttg agctgatgca ggacaaatag aagcatgttt
    17881 taaaagcata gtattaccca ctataaagtt aggggcaaat acacgcataa tctgataaaa
    17941 tgggaaattc catggctcga cagcaagtaa aacaccgaca gcttgtttca aatagtaagc
    18001 ctctccattt ggactttcta acggaacagg ttttaaaaac ttttgcccat tatcagcata
    18061 atagtcagct atgtctgcac acaagtccac ttctccttgt gcttcagtga aaagttttcc
    18121 catatcttta gtcataactt cagcgtactt atcacggtcc ttacgtaata aatctgctac
    18181 tttatgcaat tgattttggc gttcttcaac attatcttct ttgcgccatg tcttgtagag
    18241 ggcatgcgct atgtctaatg actgttcgag atcactgtca ctgatattat cgaattcatg
    18301 caatacttcg ttagtgtacg gataaattgt tttatatgcc ataatgttcc ctccttttga
    18361 tatcctcatt ataacattta tgaaatcgat tacaaatatt tagctttaga caagatcacc
    18421 aagttccaag gatagcttgc attgttaaac taactataat aatgcctaac caacacgctg
    18481 cacctaaaag aattgatttt cctccagttt taattagttt gctgacatta gtatttatac
    18541 caattgcccc cattgccata acaataaaaa atttagataa aaccttaagt ggctcaaaga
    18601 tacgtaacga aaaacctaat gaagcaacta tcgttgttag caaagaagct aagataaaat
    18661 aaagcacaaa cttcggaaat atcttagtaa cacttacaga ttgttctttc tgcgtttgtt
    18721 tcatatgata aatagataat acaatcgtaa ttggaatgat ggctaatgtt cgtgtcaatt
    18781 taacaatagt agctccgccc aaagtattgc tgtgattgat agcatcccat gcagtagcag
    18841 ttgctgtaac tgacgaggta tcattgacag cagtccctgc aaataatgca aaaccgtgat
    18901 cagagagacc aataaaattc cccagcgttg gaaaaattaa agccgctaag atattgaata
    18961 aaaaaataac cgaaatagct tgtgctactt catcatcttt agcattaata actggtgcag
    19021 tcgctgcaat tgcggatcca ccacaaatag atgaaccaac acctattaaa gtagcaatcg
    19081 ttttatctaa cttaaataac ttttgcaaaa cataagcaat aatcaatgaa atggaaatcg
    19141 tcataataat gatgggtaac gatgatatac caactttcat cacttgcatg aggtttagtc
    19201 caaaacccaa taagacaact gctgtttgta aaatatattt agacgtaaaa gcaataccct
    19261 tattaaacaa ttgtcgattt tgataaaagg atccaacaat tattccaatc aagatagcaa
    19321 agactggtgc tcctattaaa gggagataaa gcccaagcaa ccaagatggt atagcaataa
    19381 taaagcataa tattagtcca ggaatttttt ccttaaataa catgatagtc ctcctcacat
    19441 tgactattat atcacaagct ttaaaacgtg gcaggaaaac cgaaagagga ccagaatatg
    19501 ctctgatcct ctttcattac taattcacaa ttatttagtt actttcataa atggatatga
    19561 tttttcgtta cctaaaatca attttgagcc ctctttttta taggctgttt tttcaccatt
    19621 tttcacaata ttaccataga acgcatttgc ctttttattg atatgcgata ctttaataac
    19681 acttgttaag cgatgtactt taccatccat aactggcaat gtcatttcac cagtattagt
    19741 attatatttt cctccgacag cttgtgcagc actttggaaa cttctatcaa tttgttggtc
    19801 aatttgtttg tcagtgattt ttgtttcatc aaaaatcttt ttacctgctt cacttaaatc
    19861 attgtaagtc aaaccttgat cttttaattg tttttcaaga gctttatcaa taaaagtttt
    19921 tatagcttga tggaatttta cctcatcaat ttgagcagtg actgttatct ttgcctcatc
    19981 gtttttgacg ttcatatgca tattagcgcc cttaaccaaa tcagctgcac taatccccat
    20041 atccgctgcc tgacttccaa gttgttgttc tatttctttc tcaacttgct tctctaaaga
    20101 aactgtacgc catttcccct ctagtgactg acgattattc cagaaataga gaccgcctgc
    20161 acctacaatt agaataagta ctagtacagt taaaaaaact tttaaaaact ttttcatgat
    20221 aaaaactcct tatgataaac tacattaatc ataacataa
//
LOCUS       DBJYSH010000024        19883 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.024, whole genome shotgun sequence.
ACCESSION   DBJYSH010000024 DBJYSH010000000
VERSION     DBJYSH010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 19883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19883
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.024"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            <1..625
                     /locus_tag="ACUBV3_000703"
     CDS             <1..625
                     /locus_tag="ACUBV3_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011074751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HHU3847226.1"
                     /translation="LNQRQERIIDIIKQFQPITGELIAEKLGVTRAALRSDLAVLSML
                     GIIDAKPKVGYFYLGQYHASIGTSHFEKMTVSEIMGIPLTVHQKDSVYDVIVHIFMED
                     AGCAFILDDDDFLCGVVSRKDLLKTSIGGGDLSKMPIGMVMTRMPHVTTVLENESLFA
                     AADKLVSRKVDSLPVVRHDKQYPEKFKVIGKLSKTILASLFLEIRDY"
     gene            636..1466
                     /locus_tag="ACUBV3_000704"
     CDS             636..1466
                     /locus_tag="ACUBV3_000704"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HHU3847227.1"
                     /translation="MEDQLTIFIISDSLGETAKAIAKACLSQFPGHDDWHFQCFSYIN
                     SQERLEQVFEEASQKTVFMMFSLVDVALASYAQKRCESEHYAYVDLLTNVIQGISRIS
                     GIDPLGEPGILRRLDNDYFKRVESIEFAVKYDDGRDPRGILQADLVIIGISRTSKTPL
                     SMFLADKNIKVINIPLVPEVPVPKELRMIDSRRIIGLTNSVDHLNQVRKVRLKSLGLS
                     STANYASLERILEETRYAEEVMKNLGCPIINVSDKAIEETATIILEILKTNGQVAKNL
                     "
     gene            1479..4124
                     /gene="ppdK"
                     /locus_tag="ACUBV3_000705"
     CDS             1479..4124
                     /gene="ppdK"
                     /locus_tag="ACUBV3_000705"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HHU3847228.1"
                     /translation="METKFVYHFDEGCKEMKELLGGKGANLAEMTSIGLPVPQGFTIT
                     TQACNDYYDNACHIRESILSQIDQAMAQLEVEQNKQLGSVDDPLLVSVRSGAVFSMPG
                     MMDTVLNLGLNDRSVQGLVKKTEDERFAYDSYRRFIQMFADVVTGIPKYKFDTILDRL
                     KTDKCYQDDTELTGSDLQRLVEFYKELYQKEAGEKFPQDPKRQLLLAIEAVFKSWNNP
                     RAKIYRKLNDISETLGTAVNIQAMVFGNMGNNSGTGVAFTRNPSTGAANLFGEYLINA
                     QGEDVVAGIRTPQSISKLAEQMPIIYQEFVSVTQKLEAHYRDMQDMEFTIENGNLYML
                     QTRSGKRTAKAAIKIAVDQVNEGLISKEEAILRIEPKQLDQLLHPSFDLESLKKAILL
                     TTGLPASPGAAYGKVYFHAEDVVQEMKKGNPVLLVRQETSPEDIEGMVSANGIITARG
                     GMTSHAAVVARGMGKPCVAGCSQLLVDEVRREISIGHQTIKEGEMLSIDGATGNVYIG
                     QVPMAETSVDCDFEIFMKWVDENRDMMVCSNADNPRDAQKALDFGAEGIGLCRTEHMF
                     FDDERIPVVREMILADDVLSRRKALERLLSFQRDDFYQIFKVLKGKTCTIRLLDPPLH
                     EFLPHDKESIESMARQMGISTLAIEKRIQTLEEFNPMLGHRGCRLAITYPEIYQMQMR
                     ALVQGAILAMKEGYEAKPEIMIPLVSAHEEISIIRDLIEETIIEESKSKKINLSFPIG
                     TMIETPRACMIADDIAKFADFFSFGTNDLTQMSFGFSRDDAGKFLGEYVDKGLLKKDP
                     FQALDQKGIGRFIEQAVRLGKEVKPNLKIGICGEHGGEPSSIEFCYQQGLHYVSCSPF
                     RIPIAKLAAAQAKIKQSRNDITIDK"
     gene            4262..4564
                     /gene="gatC"
                     /locus_tag="ACUBV3_000706"
     CDS             4262..4564
                     /gene="gatC"
                     /locus_tag="ACUBV3_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HHU3847229.1"
                     /translation="MKISEEEVRHVANLSKLRFSDQETKEFASSLSKIVDMIELLNEV
                     DTEGVPVTTTMADRKTVMREDIAQPGNNRDDLFKNVPQHQDYYIKVPAILEDGGDA"
     gene            4564..6030
                     /gene="gatA"
                     /locus_tag="ACUBV3_000707"
     CDS             4564..6030
                     /gene="gatA"
                     /locus_tag="ACUBV3_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HHU3847230.1"
                     /translation="MSFNNQSIDQLHDLLVKKEISATELTKATLEDIHAREQAVGSFI
                     TISDDMAIAQAKEIDDKGIDADNVMSGIPLAVKDNISTKGILTTAASKMLYNYEPIFD
                     ATAVEKLYAKDMIVIGKANMDEFAMGGSTETSYFKKTNNAWDHSKVPGGSSGGSAAAV
                     ASGQVRLSLGSDTGGSIRQPASFNGIVGMKPTYGRVSRFGLFAFGSSLDQIGPMSQTV
                     KENAQLLTVISGHDVRDSTSSERTVGDFTAKIGQDIQGMKIALPKEYLGEGIAPGVKE
                     TIIKAAKHLEKLGAVIEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRTENY
                     KNLDDIYVNTRSEGFGDEVKRRIMLGTFSLSSGYYDAYYKKAGQVRSLIIQDFEKVFA
                     DYDLILGPTAPTTAFDLDSLNHDPVAMYLADILTIPVNLAGLPGISIPAGFDKGLPVG
                     MQLIGPKFSEETIYQVAAAFEATTDYHKQQPKIFGGEN"
     gene            6030..7472
                     /gene="gatB"
                     /locus_tag="ACUBV3_000708"
     CDS             6030..7472
                     /gene="gatB"
                     /locus_tag="ACUBV3_000708"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HHU3847231.1"
                     /translation="MNFETVIGLEVHVELNTNSKIFSPSSAHFGQEQNANTNVIDWSF
                     PGVLPVMNKGVVDAGIKAALALNMDIHQNMHFDRKNYFYPDNPKAYQISQFDEPIGYN
                     GWIEIELEDGTRKKIRIERAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMR
                     SPEEAYAYLTALKEIIQYTGISDVKMEEGSMRVDANISLRPYGQEEFGTKAELKNLNS
                     FNNVRKGLIHEEKRQAQVLRSGGQIQQETRRFDETTGETILMRVKEGSSDYRYFPEPD
                     LPLFDISDEWIDQVRLELPEFPQERRAKYVSSFGLSSYDASQLTATKATSDFFEKAVA
                     IGGDAKQVSNWLQGEVAQFLNSESKSIEEIGLTPENLVEMISLIADGTISSKIAKKVF
                     VHLAKNGGSAEEFVKKAGLVQISDPEVLIPIIHQVFADNEAAVIDFKSGKRNADKAFT
                     GYLMKATKGQANPQVALKLLAQELAKLKEE"
     gene            7602..8502
                     /locus_tag="ACUBV3_000709"
                     /pseudo
     CDS             7602..8502
                     /locus_tag="ACUBV3_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161968.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
     gene            8619..9146
                     /locus_tag="ACUBV3_000710"
     CDS             8619..9146
                     /locus_tag="ACUBV3_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HHU3847232.1"
                     /translation="MSIDDYRPTFVVEAVYDLRAEDLLRHGIRAVLVDLDNTLIAWNN
                     PDGTAEVRAWLDEMTTADISVVVVSNNNHARVERAVSRFGVDFVSRAMKPFTRGINMA
                     IERYGFDRDEVIMVGDQLMTDIRASHRAGIKSVLVKPIVKSDAWNTKFNRLRERRVWK
                     KIEENYGKIVYQKGI"
     gene            9146..10264
                     /gene="yqeH"
                     /locus_tag="ACUBV3_000711"
     CDS             9146..10264
                     /gene="yqeH"
                     /locus_tag="ACUBV3_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HHU3847233.1"
                     /translation="MEELFCIGCGARIQTENKDAAGYTPRAALEKGLETGELYCQRCF
                     RLRHYNEITDVHITDDEFLKLLHEVGDSDALVVNVIDIFDFNGSIIPGLSRFVAGNDV
                     LLVGNKKDILPKSVKDGKVTQWLTERAHEEGLRPVDVILTSAQNHHAIKDLIDTIEKY
                     RHGRDVYVVGVTNVGKSTLINAIIREITGSRDVITTSRFPGTTLDKIEIPLDDGSYIF
                     DTPGIIHRHQMAHYLTAKNLKYVSPKKEIKPKTYQLNSEQTLFLAGLARFDFISGQKQ
                     GFTAYFDNNLNLHRTKLVGADEFYTKHVGKLLTPPTGKEVSDFPKLVRHEFTIKNKMD
                     IVYSGLGWIRVKSEAENPVVVAAWAPEGVAVVLRKALI"
     gene            10357..10674
                     /gene="yhbY"
                     /locus_tag="ACUBV3_000712"
     CDS             10357..10674
                     /gene="yhbY"
                     /locus_tag="ACUBV3_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HHU3847234.1"
                     /translation="MLTSKQRAFLKSEAHSMKPIIQIGKNGLNDQIKTSVRNALDARE
                     LIKVTLLQNTDEDIHDVAEVLEDEIGCDTVLKIGRILILYKESARKENRKISVKVKAV
                     KSN"
     gene            10804..11436
                     /locus_tag="ACUBV3_000713"
     CDS             10804..11436
                     /locus_tag="ACUBV3_000713"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HHU3847235.1"
                     /translation="MALELLTPFTKVELEEKKRDTNRKQIGIMGGNFNPVHNAHLVVA
                     DQVRQQLCLDQVLLMPEFQPPHIDKKETIDEQHRLKMLELAIEGIDGLSIEPIEIERK
                     GISYTYDTMKLLIEKNPDVDYYFIIGADMVEYLPKWHRIDELVKMVQFVGVQRPKYKA
                     GTSYPVIWVDLPLMDISSSMIRQFIKSNRQPNYLLPKEVLDYIRKEGLYK"
     gene            11433..12020
                     /gene="yqeK"
                     /locus_tag="ACUBV3_000714"
     CDS             11433..12020
                     /gene="yqeK"
                     /locus_tag="ACUBV3_000714"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HHU3847236.1"
                     /translation="MTYKDYTGLDRTELLSKVRHMMSDKRFNHVLGVERAAIELAERY
                     GYDKEKAGLAALLHDYAKELSDDEFLRLIDKYQLDPDLKKWGNNIWHGLVGIYKIQED
                     LAIKDQDILAAIAKHTVGSAQMSTLDKIVYVADYIEHNRDFPGVEEARELAKVDLNKA
                     VAYETARTVSFLASKAQPIYPKTIETYNAYIPYLD"
     gene            12084..12605
                     /locus_tag="ACUBV3_000715"
     CDS             12084..12605
                     /locus_tag="ACUBV3_000715"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000128172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HHU3847237.1"
                     /translation="MTCALLLIDIQQGIMDKKPKHLTNFAELLDDLLLSAKGSNCEVI
                     WIRHHDEELPQGSPQWEIWERRHLVTHHKIIDKTYNSCFKDTHLHDYLQSKHISQLIM
                     MGLQTEYCFDTSVKVAFEYGYDIFIPQGGHLTFDTPTLSGDSIKKHYENIWHHRFATM
                     VAKDNLLKLINEE"
     gene            12607..12963
                     /gene="rsfS"
                     /locus_tag="ACUBV3_000716"
     CDS             12607..12963
                     /gene="rsfS"
                     /locus_tag="ACUBV3_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HHU3847238.1"
                     /translation="MTEKDLLQLVVKAADEKRAEDIVILDLQPVTSVADYFVIMSASN
                     SRQLEAIADNIREQVKGNGGDASHLEGDSKAGWVLLDLNSVVVHIFSEDERQHYNLEK
                     LWHEAPLLDAEDFMTE"
     gene            13029..13763
                     /locus_tag="ACUBV3_000717"
     CDS             13029..13763
                     /locus_tag="ACUBV3_000717"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HHU3847239.1"
                     /translation="MATYETFAAVYDAVMDDTLYDKWTDFSLRHFPKGKKKLLELACG
                     TGIQSVRFSQAGYAVTGLDLSGDMLKLAKKRATSAHQSIQFIEGNMLDLSNVGKYDLI
                     TCYSDSICYMQDEVEVGDVFIEVYKALEENGVFIFDVHSTYQTDKVFPGYSYHENADD
                     FAMVWDTYEDDAPHSIVHELTFFVQEEDGRFTRHDEVHEERTYDILTYDILLEQAGFK
                     DVKVYADFEDKKPTATSARWFFVAHK"
     gene            13786..14037
                     /locus_tag="ACUBV3_000718"
     CDS             13786..14037
                     /locus_tag="ACUBV3_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000583227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3847240.1"
                     /translation="MIIFLYACLMIFGILYIIERRVSQKKSKVILGLLMNPKLHLDLL
                     SALKPFEKETDRLIYLRKTYHLDIIDAVTLNNQLKEKEV"
     gene            14034..15143
                     /locus_tag="ACUBV3_000719"
     CDS             14034..15143
                     /locus_tag="ACUBV3_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HHU3847241.1"
                     /translation="MTVTGIVAEFNPFHNGHKYLLEQAQGIKVIAMSGNFMQRGEPAI
                     VDKWTRAQMALENGADLVIELPFLVSVQSADYFASGAVSILARLGVDNLCFGTEEMLD
                     YDRIGDIYVNKKEEMEAFLKKQSDSLSYPQKTQAMWQEFAGIAFSGQTPNHILGLAYT
                     KAASQNGIRLNPIQRQGAGYHSSEKTEIFASATSLRKHQSDRFFVEKGMPNSDLFLNS
                     PQVVWQDYFSLLKYQIMTHSDLTQIYQVNEEIANRIKSQIRYVETVDELVDKVATKRY
                     TKARIRRLLTYILINAVESPIPNAIHVLGFTQKGQQHLKSVKKSVDIVTRIGSQTWDS
                     LTQRADSVYQMGNANIAEQTWGRIPFHQSVSQSDL"
     gene            15236..15949
                     /locus_tag="ACUBV3_000720"
     CDS             15236..15949
                     /locus_tag="ACUBV3_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HHU3847242.1"
                     /translation="MKTVKEISHISGISVRTLHYYDEIDLLPPSFVGENGYRYYDDES
                     LIKLQEILLFKELEFPLKKIKEIMDSPNYDRNQALLDQIRWLELKKQRLEEVIEHAKS
                     IQRGKNMSDFTAYNQEELEAFQEEARTRWGDTDSYKEFENSYSKNDFSMISQAMSQIF
                     KDFGQLKELSPTDEKVQKQVQILQDYITAQFYNCTNDLLASLGIMYIQDERFQRSIDN
                     WGGQGTALFVSKAIDSYCQ"
     gene            16088..16492
                     /locus_tag="ACUBV3_000721"
     CDS             16088..16492
                     /locus_tag="ACUBV3_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000675692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HHU3847243.1"
                     /translation="MKGFHGNIEKLTLGNTNFRQVLYTAEHCQLVLMTLPVGGEIGSE
                     IHAENDQFFRFEAGHGKVVIDGNEYEVADGDAIIVPAGAEHNVINTSETEMLKLYTIY
                     SPAHHKDGIIRATREEAEENEEDFDGVTSETK"
     gene            complement(16758..17471)
                     /locus_tag="ACUBV3_000722"
     CDS             complement(16758..17471)
                     /locus_tag="ACUBV3_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HHU3847244.1"
                     /translation="MTQFLGEFLGTFILVLLGDGVVAGNVLSKTKEEGTGWTAIVFGW
                     GIACTVAVYVSGLFSPAHLNPAVTLAMASIGAISWGQVIPFIIAQMLGAMVAATILWL
                     HYYPHWKETKDSGLILASFSTGPAIRHTPSNLLGEIIGTAILVITIMAIGPSKVAAGL
                     GPIIVGIVIFAVGFSLGPTTGYAINPARDLGPRLMHAILPIENKGDSDWSYAWIPVVG
                     PIIGGVLGAILYNIALTLI"
     gene            complement(17481..17855)
                     /gene="dhaM"
                     /locus_tag="ACUBV3_000723"
     CDS             complement(17481..17855)
                     /gene="dhaM"
                     /locus_tag="ACUBV3_000723"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002960428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HHU3847245.1"
                     /translation="MSDIGIIVVSHSKNIAQGVVDLISEVAKDVSITYVGGTEDGEIG
                     TSFDQVQQIVEQNDKKTLLAFFDLGSAKMNLELVADFSEKNIIINSVPVVEGAYTAAA
                     LLQAGADLDSIQSQLAELTINK"
     gene            complement(17855..18433)
                     /gene="dhaL"
                     /locus_tag="ACUBV3_000724"
     CDS             complement(17855..18433)
                     /gene="dhaL"
                     /locus_tag="ACUBV3_000724"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HHU3847246.1"
                     /translation="MEVKTAIEWMHTFNQKIQSNKDYLSELDTPIGDGDHGGNMARGM
                     TAVIENLDNNEFSSAADVFKTVSMQLLSKVGGASGPLYGSAFMGITKAEQSESTISEA
                     LGAGLEMIQKRGKAELNEKTMVDVWHGVIEAIEKNELTEDRIDSLVDATKGMKATKGR
                     ASYVGERSLGHIDPGSFSSGLLFKALLEVGGV"
     gene            complement(18471..19460)
                     /gene="dhaK"
                     /locus_tag="ACUBV3_000725"
     CDS             complement(18471..19460)
                     /gene="dhaK"
                     /locus_tag="ACUBV3_000725"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HHU3847247.1"
                     /translation="MKKILNQPTDVVTEMLDGLAYVHNDLVHRVEGFDIIARNEEKSG
                     KVALISGGGSGHEPSHAGFVGEGMLSAAVCGAVFTSPTPDQVLEAIKEADEGAGVFMV
                     IKNYSGDIMNFEMAQDMAEMEGIDVASVVVDDDIAVEDSLYTQGKRGVAGTILVHKIL
                     GHAARHGKSLQEIKAIADELVPNIHTVGLALSGATVPEVGKPGFVLAEDEIEFGIGIH
                     GEPGYRKEKMQPSKALATELVDKLIESFDAKSGEKYGVLINGMGATPLMEQYVFANDV
                     AKLLEDKGIEVNYKKLGNYMTSIDMAGLSLTLIKLENQEWLEALNSDVTTIAW"
     gene            19634..>19883
                     /gene="dhaS"
                     /locus_tag="ACUBV3_000726"
     CDS             19634..>19883
                     /gene="dhaS"
                     /locus_tag="ACUBV3_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase transcriptional
                     activator DhaS"
                     /protein_id="HHU3847248.1"
                     /translation="MASSLITKKKIAKSFKRLFISQAFDKISVSDIMEDAGIRRQTFY
                     NHFVDKYALLEWIFQTELSEQVTDNLDYISGFQLLSELL"
BASE COUNT         6547 a         3173 c         4016 g         6147 t
ORIGIN      
        1 attaaatcaa aggcaagaaa gaattattga tattatcaaa cagtttcagc ccattactgg
       61 tgagctaata gctgagaaat taggagtaac aagagcagca ctaaggtctg atttggcggt
      121 tttaagtatg ctaggtatca tagatgcaaa acctaaggtt ggttattttt atttaggaca
      181 gtatcatgct tcaataggga caagtcattt tgaaaagatg acagtttcag aaattatggg
      241 gatccctctg acagttcatc aaaaagattc agtttatgat gttattgtac atatttttat
      301 ggaagatgct ggttgtgctt ttatcttgga tgatgatgat tttctctgtg gagtcgtgtc
      361 acgtaaagat ttattaaaga ccagtattgg cggaggagat ctttctaaaa tgccaatagg
      421 aatggtgatg acacgtatgc cacacgtgac aactgtttta gaaaatgaaa gtctttttgc
      481 ggcagctgat aaattagtga gcagaaaagt ggatagtctc cctgtcgttc gtcatgataa
      541 gcaatatccc gaaaaattta aagtgatcgg gaaattatct aaaacaatcc tcgctagttt
      601 gtttttagaa ataagagatt attaggagga aactgatgga agatcaactc actatattta
      661 ttatttcaga ttctttaggg gaaacagcca aagctattgc aaaagcgtgt ttgtcacaat
      721 ttccaggaca tgatgattgg cattttcaat gtttttccta tattaacagt caggaacgct
      781 tagaacaagt ttttgaagag gctagccaga agacagtatt tatgatgttt agtttagttg
      841 atgttgcttt ggcttcctat gcacagaaac gatgtgaaag tgaacattat gcttatgttg
      901 acttattaac aaatgttatt caaggaatat caaggatatc gggtatagat cctttgggag
      961 aaccagggat tttacgacgc ttagacaatg attatttcaa aagagtagaa tcaatagaat
     1021 ttgcagtaaa atatgatgat gggagagatc cgcgaggtat tttacaagca gatttagtta
     1081 ttataggaat ttcaagaact tctaaaacgc cattaagtat gtttttagca gataaaaata
     1141 ttaaagttat taatattcct ttggttccag aggttcctgt tcctaaagaa ttacgtatga
     1201 ttgattctag gagaattatc ggactaacta attcggttga tcatcttaat caagtccgaa
     1261 aagttcgatt aaagtcctta ggtctttcta gtacggcaaa ttatgccagt ttagaacgga
     1321 ttttggagga gacgaggtat gctgaagaag ttatgaaaaa tttaggttgc ccaatcatta
     1381 atgtatctga taaagcaatc gaagaaacag caacgattat tttagaaatc ttaaaaacaa
     1441 atggacaagt agctaaaaat ctgtaaaagg agacgctgat ggaaactaag tttgtgtatc
     1501 attttgatga aggttgtaag gaaatgaagg aactgctagg tggtaaggga gctaatctag
     1561 ctgaaatgac gagtattggg ttaccggtac cacaaggttt tactattaca acacaggctt
     1621 gtaatgatta ttatgataac gcttgccata tccgagagag tattcttagt caaattgatc
     1681 aagcaatggc acaattggag gttgaacaga acaagcaatt aggaagtgtt gatgatcctt
     1741 tgttggtgtc agttcggtca ggagcagttt tttcgatgcc tggtatgatg gatacagttt
     1801 taaatttagg gttaaatgat cgtagtgtcc aaggtttagt gaaaaagaca gaagacgagc
     1861 gttttgctta tgacagttat cgtcgtttta tccaaatgtt tgcagatgtg gttacaggca
     1921 ttccaaaata taaatttgat accattttag atcgacttaa gacagataaa tgttatcaag
     1981 atgacacaga actaactggt tcagatttac agagacttgt tgaattttac aaagaactat
     2041 atcaaaaaga agcaggagaa aaatttccgc aagatcctaa aaggcagctt ttgttagcaa
     2101 ttgaggcagt ttttaaatca tggaataatc caagagctaa gatttatcgt aaattaaatg
     2161 atatttcgga gacgcttgga acagccgtta atattcaagc tatggttttt gggaatatgg
     2221 gaaataacag cggaacggga gttgctttta cacgaaaccc atcaactgga gcagcaaatt
     2281 tatttggtga gtatttgatt aatgcacaag gagaagatgt tgttgcaggt atcagaacac
     2341 cacaatccat atcaaagttg gcagaacaaa tgcctattat ttaccaagag tttgtaagcg
     2401 ttacccaaaa gttagaagct cattatcgcg atatgcaaga tatggagttt acaattgaaa
     2461 atggtaatct ttatatgctt cagacgagaa gcggtaagcg tacagcgaag gcagctatca
     2521 aaatagcagt agatcaagta aatgaaggcc taattagtaa agaagaagct atactacgta
     2581 tcgagcctaa acaattagat cagttattac acccttcttt tgacttggaa agtttaaaga
     2641 aagctattct tttgacgaca ggacttccag cctctcctgg agctgcttat gggaaagttt
     2701 atttccatgc agaagatgtt gttcaagaga tgaaaaaggg aaatccagtg ctattggtca
     2761 gacaagagac atcacctgaa gatatcgagg ggatggttag tgcaaacggg attataacag
     2821 caagaggcgg aatgacctcc catgcggcag tagtagccag aggaatgggg aaaccatgcg
     2881 ttgcaggctg tagtcaatta ttggttgatg aagtaagacg agaaatttct attgggcatc
     2941 aaacgataaa agaaggtgag atgttatcaa tcgatggtgc tacaggaaat gtttatatag
     3001 gacaagttcc gatggcagag actagcgtag attgtgattt tgagatattt atgaaatggg
     3061 tagatgaaaa tcgagatatg atggtgtgtt caaatgcaga taatccgaga gatgctcaaa
     3121 aggccttgga ctttggagca gaaggaatag gtctttgtcg cacggaacat atgttttttg
     3181 acgatgaacg tattccagtc gttagagaaa tgattttagc agatgatgta ttgagtcgaa
     3241 ggaaggctct agaaaggctt ttatctttcc aaagagacga tttttatcaa atttttaagg
     3301 tattgaaagg taaaacttgt acgattcgtt tgcttgaccc tcctttacat gaattcttac
     3361 cccatgataa agagtctatc gaatctatgg caagacaaat ggggatatct actttagcta
     3421 ttgaaaaacg gattcaaaca ttagaagaat tcaaccctat gttaggtcat cgtggttgta
     3481 gacttgcgat tacctatccg gaaatttacc aaatgcagat gagagctctt gtacaaggcg
     3541 ctatacttgc aatgaaagag ggatatgagg caaagccaga aattatgatt cctttagtgt
     3601 cagcgcatga agaaatctct ataatacgtg atctgattga agagacaatt attgaggaga
     3661 gtaaatctaa aaaaattaac ttatcatttc ctattgggac aatgattgaa acaccaagag
     3721 cttgtatgat agcggatgat attgcaaagt ttgcggattt ctttagtttt gggaccaatg
     3781 atttaacaca aatgagcttt ggattttcac gagatgatgc tggaaaattc ttaggagaat
     3841 acgttgataa aggacttcta aaaaaagacc ctttccaagc tttagatcaa aaaggaatag
     3901 gaaggtttat tgagcaggca gtgaggttag gaaaagaagt taagccaaac cttaaaatag
     3961 gtatctgtgg agagcatggt ggagaaccat catctattga gttctgttac caacaggggt
     4021 tacattatgt atcgtgttcg ccgtttagaa ttcctattgc taaattagcg gctgcacagg
     4081 ctaaaatcaa acaaagtaga aatgatataa caatagataa ataaacagta aacgcttctt
     4141 atcataggta agaagcgttt attatgaaaa gctgggataa tgtgataaca ttgagagttt
     4201 aatatgctat aatagtagag actatcttaa aacgaaaatg atacagatat aaggagaact
     4261 catgaaaatt tctgaagaag aagtgcgtca tgttgccaat ctgtcaaaat tgcgcttttc
     4321 tgatcaagag actaaagaat ttgcgtcaag tttgtcaaag attgttgata tgattgaact
     4381 cttaaatgaa gttgacacag aaggcgtacc agtaacaacg accatggcag atcgcaaaac
     4441 cgtgatgcgt gaagatattg ctcaaccagg caataatcgt gatgacttat ttaaaaatgt
     4501 accacagcat caagattatt atattaaagt acctgcaatt cttgaggatg gaggagatgc
     4561 ctaatgtcgt ttaataatca atcaattgat cagttgcatg atcttctcgt taaaaaagaa
     4621 atttcagcaa ctgaattaac aaaagctaca ttggaagata ttcatgcgcg tgaacaagcg
     4681 gttggatctt ttattactat ttctgatgat atggcaatag ctcaagccaa agaaattgat
     4741 gataaaggaa ttgatgctga taatgttatg tcaggtattc ccttagcagt taaagataat
     4801 atttcaacaa aggggattct tacaacagct gcttctaaaa tgctctataa ctatgagcca
     4861 atctttgatg ctacggctgt tgaaaagtta tatgctaaag atatgattgt gattggtaaa
     4921 gcaaacatgg atgaatttgc catgggaggc tctacagaaa catcttattt taaaaagaca
     4981 aataacgctt gggaccatag taaagttccg ggaggatcat caggtggttc tgcagcagca
     5041 gtagcttcag gacaagtacg tttgtcattg ggctctgata caggtggttc aattcgccag
     5101 ccggcttcat ttaatggaat cgttggtatg aagccaactt acggacgtgt atcacgcttt
     5161 ggtttgtttg ctttcggtag ttcgcttgac cagattggac cgatgtctca aacagtaaaa
     5221 gagaatgcac agcttttgac agttatttct gggcatgatg taagagattc aacttcttct
     5281 gagcgtactg taggcgattt tactgctaaa attggtcaag atattcaagg aatgaaaatt
     5341 gctttaccaa aagaatattt gggagaagga atagcgccag gagttaaaga aacgattatt
     5401 aaggccgcaa aacatctgga aaaactaggt gcagttattg aggaagttag tttaccgcat
     5461 tctaaatacg gtgttgctgt ttattatatt attgcttcat cggaagcttc ttcaaaccta
     5521 caacgttttg atggtattcg ttatggatac cgtacagaaa attataaaaa cttggatgat
     5581 atctatgtca atacacgatc tgaaggtttt ggagacgaag ttaagcgtcg cattatgctg
     5641 ggaacgttca gtttatcttc tgggtattac gatgcttatt ataaaaaagc aggtcaagtt
     5701 cgtagtttga ttattcaaga ttttgaaaaa gttttcgcag attatgactt gattttaggt
     5761 ccaacagctc caacaacagc ctttgactta gattctttaa atcacgatcc agttgcaatg
     5821 tacctagctg atattctaac aataccagtt aatttggctg gtttaccagg tatttcaatc
     5881 cctgctggtt ttgataaagg tctaccggtt ggtatgcagt tgattggtcc gaaattcagt
     5941 gaagaaacaa tatatcaggt tgctgctgct ttcgaagcaa cgacagatta tcataaacaa
     6001 caacctaaaa tttttggagg tgagaactaa tgaattttga aacagtcatt ggacttgaag
     6061 tccatgttga attaaatacg aactctaaaa ttttctcacc ttcttccgct cattttggtc
     6121 aggaacaaaa tgctaataca aatgtaattg actggtcttt cccaggtgtc cttccagtca
     6181 tgaataaagg agttgttgat gcaggaatca aggcagcact tgccttgaat atggatatcc
     6241 atcagaacat gcattttgac cgtaaaaatt atttctatcc agataatcct aaagcttatc
     6301 aaatttcaca gtttgatgag cctattggtt acaatggttg gattgaaatc gaattagaag
     6361 atggtacacg aaaaaaaatt cgtattgagc gtgcccacct cgaagaagat gcaggaaaaa
     6421 atacacatgg gacagatggt tattcctatg ttgaccttaa tagacaaggt gttccactga
     6481 ttgaaattgt atcagaagct gatatgcgtt ctcctgagga agcttatgct tatttgacag
     6541 ctctcaaaga gattatccaa tatactggaa tttctgacgt taaaatggaa gaaggttcta
     6601 tgcgtgtaga tgccaatatt tcacttcgtc cttatggtca agaagagttt ggtactaaag
     6661 cagaacttaa gaaccttaac tcattcaata atgtacgtaa aggtttgata catgaagaaa
     6721 aacgtcaagc acaagtttta cgatctggtg gtcaaattca gcaagagact cgtcgttttg
     6781 atgaaacaac aggtgagact attttgatgc gtgtcaagga aggctcatca gattaccgct
     6841 atttccctga accagatcta ccactatttg atatctcgga tgaatggatt gatcaagttc
     6901 gtttagaatt accagaattt ccacaggaac gtcgtgcgaa atatgtatcg tcttttggtc
     6961 tatcatctta tgatgctagc caattaacag ctacaaaagc aacttctgat ttctttgaaa
     7021 aagctgttgc tattgggggt gatgcaaaac aagtttccaa ttggttacaa ggagaagtgg
     7081 cgcaatttct taacagtgaa tcaaaatcaa tcgaagaaat tggtctgact cctgagaatt
     7141 tggttgagat gattagcttg attgctgacg gtacaatttc atccaaaatt gctaaaaaag
     7201 tttttgtgca tttagctaaa aatggtggtt cagctgaaga attcgttaaa aaggctggtt
     7261 tagttcaaat ttcagatcct gaagtattaa tcccaattat tcatcaagtc tttgcagata
     7321 atgaagctgc tgtgattgac tttaagtcag gaaaacgcaa tgctgataag gccttcacag
     7381 gttatctaat gaaagcaaca aaaggacaag ctaatccaca agttgctctg aagctgttag
     7441 cgcaagaatt agcaaaatta aaagaagaat agaaatcagc cttttggctg gtttttattc
     7501 gctaatttag cacgaacgcc tagggataaa taattaagaa gctaagatat ttatcagatg
     7561 actctaacta cagtgtttaa gaaataaaaa tgaggggaaa aatgactaaa aaagaaaaag
     7621 gaacaatgat gaccttagct gccggactag catggggcat ttctggaatt agtggtcaat
     7681 acttgatgtc gcacggagta cacgttaacc ttttgacttc tttacgattg ttgataacag
     7741 gtatctttct cttgtcttta gctagatcaa aacaaaagga gcatttagta gcagcgtgga
     7801 aacagccaaa atttttaaag caggttttac ttttttctat atttggttta gttttaaatc
     7861 aatatgcttt tctcagagcg attcatttaa caaatgctgg aactgcaacg gttttgcaat
     7921 atatggcacc aatacttatt ttgagtattg tttgtatcct aaacagacaa agaccaacat
     7981 cttttgaaat aatagcaatt gctatggcaa ttttaggaac ctatatgatt gcaacgcatg
     8041 ggaaattagg aagtttagct atcacaccga aaggattaat gtgggggctt ggttcagcca
     8101 ttacttattc tatttatatt ttattacctg taaaacttat tcatgaatgg ggaagtacga
     8161 ttgttattgg ttcaggaatg tttataggtg ggattttatt tagtttggtg acaaaagcgt
     8221 ggcaataccc cttacaaatt aatgtgatga gtatacttgc ttatattggc attattggta
     8281 ttgggaccat ttttgcttaa aggggttagt attgtaggtg ctgtcaaggg tagcttatta
     8341 gcatcggtag agccagtttc ttcggtgttt ttgacagtac ttgttttagg agaaattttt
     8401 tatccaatag atttacttgg gatgttgttt attttccttg ctgtaactct aatctcttac
     8461 aaagatttaa ttgcattgaa gaaaatgaaa cgagtaaaat agtatctttt ggggttgaaa
     8521 ccctaattta aaggtcttta aagacctttt ttctttactc gctatctctt gagaattatg
     8581 gtagaatgaa tagtaaaata tattttttgg agatgaaatt gagtatagat gattataggc
     8641 cgaccttcgt ggttgaggca gtttatgatt taagagcaga agatttatta cgtcatggca
     8701 ttcgtgcggt tttagttgat ttagataata cactcattgc ttggaataat ccagatggta
     8761 ctgctgaggt gcgtgcatgg ttagatgaga tgacaacagc ggatatttca gttgtagtag
     8821 tgtctaacaa caatcatgct cgcgtagaac gtgcagtatc acgttttggt gtggattttg
     8881 taagtcgtgc gatgaaacca tttactcgtg gtataaacat ggcaattgag cgttatggct
     8941 ttgatcgtga cgaggtgatt atggttggag atcaattgat gacagatatt cgtgctagtc
     9001 atcgcgcagg aataaagtcc gtattggtaa aaccaattgt taaatctgat gcttggaata
     9061 ccaagttcaa tcgcttacgt gaacgtcgcg tttggaaaaa aatagaagag aattacggta
     9121 aaatcgtata tcaaaaagga atttaatgga agagttattt tgtattggct gcggagccag
     9181 gattcaaact gaaaataaag atgctgctgg ctatacacct cgtgcagcac ttgaaaaagg
     9241 tttagagaca ggagaattat actgtcagcg ttgtttcaga ttgcgccact ataatgagat
     9301 tactgacgtt catatcactg atgatgaatt tttgaaatta ctacatgagg ttggagatag
     9361 tgatgccctt gtggtgaatg tcattgatat ttttgatttc aatggttcaa ttattcctgg
     9421 tcttagtcgt ttcgttgcag gaaacgatgt tcttttagta ggaaataaaa aagatattct
     9481 tcccaaatcg gttaaagatg gaaaagtaac acaatggtta actgagagag ctcatgaaga
     9541 ggggcttcgt cctgttgatg tcatcttgac aagtgcccaa aatcatcatg ctattaagga
     9601 tttgattgat acgattgaaa agtatcgtca tggtcgagat gtttatgttg ttggggttac
     9661 taatgttgga aagtcaactc ttattaacgc tattattcgt gaaatcactg gtagtcgtga
     9721 tgttattacg acctcacgtt tcccaggtac aacattagat aaaatagaaa tacctcttga
     9781 tgatggtagc tatatttttg ataccccggg tattattcat cgtcatcaaa tggctcatta
     9841 tttgacagca aaaaatctta aatatgttag tcctaaaaaa gaaataaaac ccaaaactta
     9901 tcagcttaat tcagaacaaa cattattctt ggctggccta gcacgttttg attttatttc
     9961 tggtcaaaaa caaggtttta cggcatattt tgataataac ctgaatttac accgcactaa
    10021 actagttgga gcagatgaat tttacactaa acatgttgga aaattattaa caccaccaac
    10081 aggtaaggaa gtttcagatt ttcctaaatt agttcgtcat gaatttacaa tcaagaataa
    10141 aatggatatt gtttattcgg gccttggttg gatacgtgtg aaatcagaag cagaaaatcc
    10201 agtagtagta gctgcttggg ctccagaagg cgtggcagtt gttttacgca aagctttaat
    10261 ttaataacta aattaaccag caacatatga cgatttttca tctaatttaa ctgatgatac
    10321 agtcataatc ctataataga taaaaaggaa actaatatgt taacatctaa acaacgtgct
    10381 ttcttaaaat cagaagcaca ttctatgaaa cccattattc aaattgggaa aaatggttta
    10441 aatgatcaaa tcaaaacaag cgtacgtaat gcactcgatg cacgtgagct aatcaaagta
    10501 acccttttac aaaataccga tgaagatatc cacgatgtcg ctgaagttct ggaagatgaa
    10561 attggctgcg atactgtcct taaaatagga cgtatcttga ttctttataa agagtcagcg
    10621 cgcaaagaaa atcgtaaaat ttcagttaaa gtaaaagcag taaaatcaaa ctaaactaca
    10681 atagattaag aaatagagta tagaactaaa aaacgattta cctaagttac acttccgtat
    10741 gatagctgat catgataatc agtgtttgtt tttttagaga tactacttgt tcatggagga
    10801 attatggcac tcgaattgct aacaccattt acaaaagttg aattagaaga gaaaaagcga
    10861 gatactaaca gaaaacaaat tggaattatg ggaggaaatt ttaaccctgt tcataacgcc
    10921 caccttgttg ttgcagatca agtacgtcag caattgtgtt tagaccaagt tctgttgatg
    10981 cctgaatttc agccaccaca tattgataaa aaagagacaa ttgatgaaca gcatcgtctc
    11041 aagatgcttg aattagcaat agaaggcatt gatggtttat ctattgaacc gattgaaatt
    11101 gaacgtaaag gtatcagcta tacatacgat acgatgaagc ttctaattga aaaaaatcca
    11161 gatgttgatt attatttcat tattggggca gatatggtgg aatatctacc taagtggcat
    11221 cgtattgatg aacttgttaa aatggtgcag tttgttggtg ttcaacgtcc aaaatataaa
    11281 gctggtacat catatcccgt tatttgggtg gatctgccat tgatggatat ctcatcaagt
    11341 atgattcgtc aatttattaa aagtaatcgt cagcccaact atttactgcc taaagaggta
    11401 ttagattata tcaggaaaga aggtctttac aaatgaccta caaagattac acaggtttag
    11461 atcggactga acttttgagt aaagtgcgtc atatgatgtc cgacaagcgc tttaatcatg
    11521 tgttaggagt agagcgtgct gcaatagaat tagcagaacg gtatggttat gataaggaaa
    11581 aagcgggcct agctgcttta ttacatgatt atgctaaaga actatctgat gatgaatttc
    11641 ttagattaat tgataaatac caactagatc ccgacctaaa aaaatggggt aataatattt
    11701 ggcatggtct tgttggcatt tataaaatac aagaggatct agctatcaaa gatcaagata
    11761 ttttagctgc tattgcaaaa catactgttg gttcagccca aatgtccaca ttggataaaa
    11821 tcgtttatgt agcagattac attgaacaca atcgagattt tccaggagtt gaagaagctc
    11881 gtgagttagc gaaagttgac cttaataaag cagtagctta tgagactgct agaacagttt
    11941 cgtttttagc aagtaaggct cagcctatct atcctaaaac gattgaaacc tataatgctt
    12001 atatacctta tctcgactaa ttcttttatt gaaataatgg tcatgatagg tttggcgatg
    12061 cagtggtcat tttggaggaa agtatgactt gtgcactttt actaattgat atccaacaag
    12121 gtattatgga taagaaacca aagcatttaa caaattttgc agagctttta gatgatttac
    12181 ttttaagtgc caaaggttct aattgtgaag tgatatggat aaggcatcat gatgaggaat
    12241 tgcctcaagg ttctccacag tgggaaattt gggaacggcg acatcttgtg acgcatcaca
    12301 aaataattga taaaacttac aatagttgtt ttaaagatac tcatttacat gattacttgc
    12361 aaagtaagca tattagtcaa ttgatcatga tgggacttca aacagaatac tgttttgata
    12421 catcagtaaa agttgctttt gaatatggtt atgatatctt tattccacaa ggtggacacc
    12481 taacatttga tacaccaact ttaagtgggg attctattaa aaaacattac gagaatattt
    12541 ggcatcatcg ttttgcgaca atggtagcaa aagataactt attgaaatta ataaacgagg
    12601 aataaaatga cagaaaaaga cttactacag ctagtcgtta aggctgcaga tgaaaaacga
    12661 gcagaagata ttgtaatttt agatttacag cctgtaacga gtgttgcaga ttactttgtg
    12721 attatgagtg caagtaatag taggcagtta gaagcaatcg cagacaatat ccgtgaacaa
    12781 gtaaaaggaa atggtgggga tgctagccac cttgaaggcg atagtaaagc tggttgggta
    12841 cttcttgacc tcaatagcgt cgtggttcat atcttctctg aagatgagag acagcactat
    12901 aaccttgaaa aactttggca tgaagcacca ttgcttgatg ctgaagactt tatgacagaa
    12961 taaagctaag cagtcttggg actgcttttt gagagatgta atatcaagaa agatttgaga
    13021 actattatat ggcaacgtat gaaacttttg cagctgttta tgatgctgtc atggatgata
    13081 ctttatatga taaatggact gatttttcac ttcgtcattt tccaaaaggt aagaaaaaac
    13141 ttttggaatt agcatgtggt acgggaattc agtctgttcg attttcacaa gctggttatg
    13201 ctgttacagg acttgatctt agtggcgata tgctgaaact tgccaaaaaa cgtgcaactt
    13261 ctgctcacca aagtatccag tttattgaag gaaatatgtt agatttatct aatgttggta
    13321 aatatgatct catcacgtgc tattcagact ctatttgtta tatgcaagat gaagtcgagg
    13381 ttggtgatgt ttttatcgaa gtctataagg ccttagaaga aaatggcgtc tttatttttg
    13441 atgttcattc gacttatcaa acagataaag tatttcctgg ttattcttat catgaaaatg
    13501 cagatgactt tgctatggtg tgggatactt atgaagatga tgcacctcat tctattgtcc
    13561 atgaattaac attttttgtt caagaagaag atggtcgatt tacacgtcat gatgaagttc
    13621 atgaagagcg tacttatgat attttgactt atgatatttt acttgaacag gcaggtttta
    13681 aagatgttaa ggtgtatgca gattttgaag ataaaaaacc aactgcaact tctgctcgct
    13741 ggttttttgt agctcataaa taagtaataa tttggaggta aaaagatgat aatattctta
    13801 tacgcttgtc tgatgatatt tgggatactt tatattattg aacgacgggt aagtcagaaa
    13861 aagtctaaag ttattttggg attattgatg aaccctaaac ttcatttgga tttattatcg
    13921 gcattaaaac catttgaaaa agaaacagat cgtctcatat accttcgtaa aacttatcac
    13981 ctagatatta ttgatgctgt tactttaaat aatcaattga aagagaaaga agtatgacag
    14041 ttacaggaat tgttgctgaa tttaatcctt ttcataatgg ccataagtac cttttagaac
    14101 aagctcaagg aataaaagta atagccatgt cgggtaattt tatgcaacgt ggtgagccgg
    14161 cgattgtaga taaatggacc cgtgcccaga tggcactaga aaatggtgct gatttagtga
    14221 ttgaactccc ttttttggtt tcggtacagt cagcggatta tttcgctagt ggagctgtgt
    14281 ctatcctggc acgattaggt gtggataact tatgcttcgg taccgaagaa atgcttgatt
    14341 atgaccgtat tggggatatt tatgtgaata aaaaagagga gatggaagct tttttgaaaa
    14401 aacaatcgga ttctctttcc tatccacaaa aaacgcaagc tatgtggcaa gaatttgcag
    14461 ggattgcatt ttcgggacaa accccaaatc acattttagg cttagcctac accaaagcag
    14521 ctagccaaaa tggtattcga ttaaatccga tacaacgtca aggagctggt tatcattcat
    14581 cagaaaaaac agagatattt gcttccgcaa caagtcttcg taaacatcaa tctgatcgtt
    14641 tctttgtaga aaaaggtatg cctaacagtg acttgttttt gaatagtcca caagtagttt
    14701 ggcaagatta ttttagttta ctcaaatatc agataatgac gcattctgat ttgacacaaa
    14761 tttatcaagt gaatgaagag atagccaatc gtataaaaag tcaaattcgt tatgtggaga
    14821 ctgtggatga gttagttgat aaagtcgcaa ctaaacggta taccaaagca agaattcgac
    14881 gtttattaac ctatatcttg ataaatgctg tggaaagtcc tataccaaat gctattcatg
    14941 tcttaggttt tacacaaaaa ggtcaacagc atttaaagtc tgtgaagaag tctgtggata
    15001 tcgtgacgag gattggttca caaacgtggg atagtttgac gcaacgagca gattctgttt
    15061 atcagatggg aaatgctaat atagcagagc aaacatgggg acgtatccct tttcatcaat
    15121 ctgttagtca aagcgacctt tgatttaagc tttataaatt aatcatcaca aaataatatt
    15181 tttcccttga cccttacgta acgttataca ttatactctt tttagagagg ttattatgaa
    15241 aacagttaaa gaaataagtc acatttctgg tattagtgtg cgcacacttc attattatga
    15301 tgaaatagat ttgttacctc cttcatttgt aggagagaat ggttatcgtt actatgatga
    15361 tgaatcactg attaagttac aagaaatttt gttatttaag gaactggaat ttcctcttaa
    15421 aaaaataaaa gagataatgg atagtccaaa ttatgatcgt aatcaagcac ttcttgatca
    15481 aattaggtgg ctggaactaa agaaacagcg actagaagaa gtaattgaac atgctaagtc
    15541 tatccagagg ggtaaaaata tgtcagattt tacagcttat aatcaagaag aattagaagc
    15601 atttcaagag gaagctagaa cacgctgggg ggatacagat agttacaaag aatttgaaaa
    15661 tagctatagt aagaatgatt tttcaatgat ttcacaagct atgagtcaga tttttaaaga
    15721 ctttggacag ttgaaagaac tttctccgac agatgagaaa gttcaaaaac aagttcagat
    15781 tttacaagat tatatcacag ctcagtttta caactgcact aacgatttat tagcaagtct
    15841 tggaataatg tacatccaag atgagcgttt ccaaaggtct attgataatt ggggaggcca
    15901 gggaacagca ttatttgttt ctaaagctat agatagctat tgtcagtagg ctatatttta
    15961 ggtttgcaat gaaatgatag gaatcaaggg ggagtagata ttattaaaaa tgtcagactc
    16021 ttatctaaat ttttgacagt aagtggcaaa aagagtagac tgtaggcata gaaaggtagg
    16081 taccgttatg aaaggatttc atgggaatat cgaaaaattg acactaggaa atacaaattt
    16141 ccgccaagtc ctctataccg cagaacattg tcaactcgtt ttaatgacac tgcctgttgg
    16201 tggagaaatt ggtagcgaga ttcatgcaga aaatgatcag tttttccgtt ttgaagctgg
    16261 tcatggtaag gttgtcattg atggtaatga atatgaagtt gcagatggag acgctataat
    16321 agttccggca ggtgctgaac ataacgtaat taacacttct gaaaccgaga tgttaaaact
    16381 ctatacaatt tacagtccag ctcatcacaa agatggtatt atccgtgcca caagagaaga
    16441 agctgaagaa aatgaagaag attttgatgg agttacttct gaaactaaat aatactagac
    16501 taaaaagcgc tatgtcaata gcgcttttta gcttaacagt agtcatttct tggagaatat
    16561 aaatagtaat ttaggaagta atgttaaata gccgagcttt taaggaaacg ttaagtaagg
    16621 gcggttatac tataaacatc ctaaactttt ttcataaaat ctccagaaag aacactaagt
    16681 attttggtgt tttttctatt ttgaaagcat actgcaacta cgaaaaacca gaaagttaat
    16741 actttctggt tttttagtta aattaaagtt aaagcaatat tgtataagat agcgccaagg
    16801 acaccgccga taattggacc tacaactgga atccaagcgt atgaccaatc tgagtcgccc
    16861 ttattttcaa taggtaagat agcatgcata agacgaggcc ctaggtcacg tgcagggtta
    16921 atagcataac cagttgttgg accaagagag aatcctactg cgaaaataac tataccaacg
    16981 ataattggac caagacctgc tgcaacttta ctaggaccaa ttgccataat tgtaatcaca
    17041 agaatagcag taccgataat ttcaccaagg agattcgatg gtgtatgacg gatagcaggt
    17101 ccggttgaga agctggcaag gataagtcca ctatcttttg tttctttcca atgaggataa
    17161 tagtgtaacc ataaaatcgt tgccgcaacc attgctccta acatctgtgc aatgataaag
    17221 gggataactt gtccccaaga aattgctcca atagaagcca ttgctaatgt gactgctgga
    17281 tttagatgag caggactaaa taatccagaa acataaacag caacggtaca agcaattccc
    17341 caaccaaaaa caatagcagt ccatccagtt ccttcttctt tggttttact gagcacattt
    17401 cctgcaacta cgccatcccc aagaagaacc aaaataaatg ttcctaaaaa ttccccaaga
    17461 aattgagtca taatatctcc ttatttattg atagttaatt cagctaattg tgattgaata
    17521 ctatccaggt cagcacctgc ctgtagaaga gcagcagcgg tgtaagctcc ctccactact
    17581 gggacagagt taatgataat atttttttct gaaaaatcag ctactaactc taaattcatt
    17641 tttgctgaac ctaaatcaaa gaaagctaat aacgtctttt tatcattttg ctctacaatc
    17701 tgttgaactt gatcaaaaga agtaccaatc tcaccatctt ctgttccacc cacgtaagtt
    17761 attgagacat cctttgctac ctctgaaata agatcaacaa ctccttgagc aatattttta
    17821 gagtgagaaa caacaataat accaatatca gacattagac acctccaact tctagtaatg
    17881 ctttaaataa taatcctgaa gaaaatgacc ctggatcaat gtgtcctaat gagcgttctc
    17941 caacataaga agcgcgtcct tttgttgctt tcatcccttt tgtagcatct actaaggaat
    18001 caatacggtc ttcagtcaat tcattttttt caatcgcttc aataacaccg tgccaaacat
    18061 caaccattgt tttttcatta agttcagctt taccacgttt ttgaatcatt tcaagtcctg
    18121 cgccaagtgc ctctgaaatg gtactttcac tctgttcagc ttttgtaatt cccataaatg
    18181 cagaaccata gagtggtcca gatgctccgc ctactttgct taaaagttgc atggatacgg
    18241 ttttgaaaac atcggcagca ctactaaatt cattgttatc aaggttttca ataacagcag
    18301 tcataccacg cgccatattg ccaccatggt cgccatcacc tattggagta tctagttcgg
    18361 ataaatagtc cttgttggat tgaatttttt ggttaaaagt gtgcatccat tctattgcag
    18421 tttttacttc cataagagtt cctttctttt gttctaatta acaggaatga ttaccaagca
    18481 atggttgtaa cgtcactatt aagtgcttct aaccactctt gattttccag tttaatcaag
    18541 gttagtgata aaccagccat atcaattgaa gtcatatagt taccaagttt tttgtaattc
    18601 acttcaatgc ctttatcttc aaggagtttg gcaacatcgt tagcaaagac atattgttcc
    18661 ataagtgggg ttgcacccat accgttaata aggacaccat atttttcgcc agatttagca
    18721 tcgaacgatt caattaactt atcaactaat tctgtcgcta aagcctttga aggttgcatt
    18781 ttttctttac gataacctgg ttcaccatga ataccgatac caaattcaat ttcatcttct
    18841 gcaagaacaa atcctggctt gcctacttca ggaacagtag ccccgcttag tgcaagtcca
    18901 acagtgtgaa tgtttggtac taattcatca gcaatagctt tgatttcctg aagtgatttg
    18961 ccatgacgag cagcatgtcc tagaatttta tggacaagta tcgtaccagc tacaccacgt
    19021 tttccttgtg tgtaaagact gtcttcgact gcaatatcat catcaacgac cacacttgca
    19081 acgtcaatgc cttccatttc agccatatct tgtgccattt caaagttcat gatatctcct
    19141 gaataatttt tgataaccat gaaaactcca gctccctcat cagcttcttt aattgcttca
    19201 agaacttggt caggagttgg agaagtaaat actgcaccac acacagctgc agataacatt
    19261 ccttcgccta cgaaaccagc atgtgaaggc tcatgtccac ttcctccgcc agaaatgaga
    19321 gccacttttc ctgacttctc ttcattgcga gcaatgatat caaaaccttc tacgcgatgt
    19381 actaaatcat tatgaacata tgctaatcca tctagcattt cagtaacaac atcagttggt
    19441 tgattgagaa tttttttcat ttggaaactc cttttatgtt taattctctt ttattataaa
    19501 atttgtaagc cttttccgga atggacagat tttcttgttt gtccgctttg ttagggtttt
    19561 caataatcat aaatcatgtt acactaaacc ataataaatc atcagcaatt caatataaaa
    19621 taaaggagaa attatggctt cttcgcttat tactaagaaa aaaatagcca agtcatttaa
    19681 acgtttattt atttcgcaag cttttgacaa aatatcggtg agtgatatta tggaagatgc
    19741 aggtattaga cgtcaaacat tttataatca ctttgttgat aaatatgctt tattagaatg
    19801 gattttccaa acagaattaa gcgaacaagt tacggataat ttagactata tctcaggttt
    19861 tcagttgtta tcagagttgt tga
//
LOCUS       DBJYSH010000025        19828 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.025, whole genome shotgun sequence.
ACCESSION   DBJYSH010000025 DBJYSH010000000
VERSION     DBJYSH010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 19828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19828
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.025"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            complement(46..861)
                     /locus_tag="ACUBV3_000727"
     CDS             complement(46..861)
                     /locus_tag="ACUBV3_000727"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HHU3847249.1"
                     /translation="MAKCLTLNTHSWMEVNALKKLFDLAEHIFREKYDIICLQEVNQS
                     ISSPLAKSSPNYHPIEGTPALHQDNFALQLVHYLNLQGLHYHWTWAYNHIGYSKYHEG
                     VAILSLKPLKPEDILVSAVDDETDYHTRRALVAETTLNDKVVTVVSLHLSWFEKGFAE
                     EWKRLETTLLEVETPLLLMGDFNNPTGNQGYELVLNSPLALKDSHQIANHVFGDHTIM
                     ADIDGWEGNKKALKVDHIFTSEDLSISSSQVVFEGGEAPVVSDHYGLEITMDF"
     gene            complement(915..3098)
                     /locus_tag="ACUBV3_000728"
     CDS             complement(915..3098)
                     /locus_tag="ACUBV3_000728"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIBC"
                     /protein_id="HHU3847250.1"
                     /translation="MKNNVKQLFSFEFWQKFGKALMVVIAVMPAAGLMVSIGNSISLL
                     DPSNVLLGRIANVIAQIGWGVIGNLHILFALAIGGSWAKERAGGAFAAGLSFILINLI
                     TGNFFGVKTDMLADSKATVQTVFGATIRVSDYFVNVLGQPALNMGVFVGIISGFVGAT
                     AFNKYYNYRKLPDALTFFNGKRFVPFVVIYRSVIVALILSVFWPVVQSGINGFGKWIA
                     SSQDSAPILAPFVYGTLERLLLPFGLHHMLTIPMNYTQLGGTYTVLTGATKGAQVLGQ
                     DPLWLAWVGDLINLKGSNSSQYHHLLTSVTPARFKVGQMIGASGILMGLSYAMYRNVD
                     KDKKLKYKSMFISAAAATFLTGVTEPIEYMFMFAAMPLYLVYAVVQGCAFAMADIVNL
                     RVHSFGNIEFLTRVPMGIKAGLGGDIFNFVWVTLLFAVLMYFIANFMIKKFNLATAGR
                     NGNYDNEEVDNAPSTASGSADANSQVVQVINLLGGRDNIEDVDACMTRLRVTVKDGNS
                     VGSEAAWKKAGAMGLVLKGNGVQAIYGPKADVLKSDIQDLLDSGTVIPIVDLETGQPV
                     AAAPVTTYKGITEEIVSVANGQVEALDVVKDPVFSQKMMGDGFAVEPTDGNIYVPVSG
                     TVTSVFPTKHAFGLLTESGLEVLVHIGLDTVALDGQPFEVKISSGQKVVAGDLAVVAD
                     LEAIKAAGKETSVIIVFTNVSDIKTVKLEKSGPQIAKTVVAKVEL"
     gene            complement(3256..4911)
                     /locus_tag="ACUBV3_000729"
     CDS             complement(3256..4911)
                     /locus_tag="ACUBV3_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HHU3847251.1"
                     /translation="MTKKIFRTTLSASLGIVLVTILMIMGFLYNYFNHIQREQLRTQT
                     ALASQGISFEGKDYFENLKTSNVRITWVDNKGQVLYDTQSDAKHMKNHANRQEIKEAI
                     KSGYGESTRWSATLTEKSIYAAQRLNNGTIVRLSVAQQTIFYLLLGMISPLAIIILLA
                     IILSVLIARYIAKKVSEPLNNIDLDHPLSNDSYEEITPLLRRLDSHQAKIQHQKLLLQ
                     KRQKEFDTIISKIKEGMILLDDQARIVSINAEALKLFQINDDWHGRFMMEVSRDLTLK
                     DLIDQGLKGKKKEANIGIENNHYRVLVRPTTDNNRVTGLVVLLFDVTDQLQMEQLQRE
                     FTANVSHELKTPLHVISGYSELLANQMVPNEEVPQFAAKIHKESERLVKLVEDIINLS
                     HLDEQEKLPQETVNLYDLTQKVLEGLQAKADKKHIQINFNGEEAILRGNPVLLNSLVY
                     NLCDNAITYNHEKGQVNVTLKNSPDTITLEVSDTGLGIAEKDKKRIFERFYRVDKSRS
                     KIVGGTGLGLSIVKSALDFHNGSIKVDSHLGQGTTMTVLLHKQ"
     gene            complement(4904..5581)
                     /locus_tag="ACUBV3_000730"
     CDS             complement(4904..5581)
                     /locus_tag="ACUBV3_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001931721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HHU3847252.1"
                     /translation="MIYCVEDDADIREMMLYTLQMAGFKAQGFSSSELFWEAIQEKVP
                     DLILLDIMLPGDDGLTILERLRRKHQTEMIPVIMTTAKGSEYDKVKGLDLGADDYLVK
                     PFGMMEMISRIKAVLRRSRQVDSKAHIIIGNLEIDPTNYWVKRGTEKIHLTLKEFELL
                     VLFFRNPNRVFTRQELLDKVWGEQFLGETRTVDVHIGTLRTKLGEDGYLIATVRGVGY
                     RLEERHD"
     gene            complement(5581..6237)
                     /gene="phoU"
                     /locus_tag="ACUBV3_000731"
     CDS             complement(5581..6237)
                     /gene="phoU"
                     /locus_tag="ACUBV3_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000621378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HHU3847253.1"
                     /translation="MIRSRFASQLNDLNKEIIFMGALCEDIIGKSLGALTNSNDVYLD
                     DISETYHKIEQMERDIEERCLKLLLRQQPVAKDLRRISSALKMVYDMKRIGAQAYEIA
                     EIVSLGHIIQGAGSERDQLNSMSNNVISMLTKSIDAFIYDNEEQAHQVIEQDRTVNQE
                     FDTIKKQLVLYFSVQDVDGEYPIDVLMIAKYLERIGDHTVNIAKWVLFSITGNLDGEA
                     "
     gene            complement(6234..6983)
                     /gene="pstB"
                     /locus_tag="ACUBV3_000732"
     CDS             complement(6234..6983)
                     /gene="pstB"
                     /locus_tag="ACUBV3_000732"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HHU3847254.1"
                     /translation="MSKLVINNLDLYYGEFHALKDVNLDIEEKEITAFIGPSGCGKST
                     LLKSINRMNDLVKNCKITGDITLEGEDVYRQLDINQLRKKVGMVFQKPNPFPMSIYDN
                     VAFGPRTHGIHSKAELDDIVERSLKQAALWDEVKDRLHKSALGMSGGQQQRLCIARAL
                     AIEPDVLLMDEPTSALDPISTAKIEELVIQLKKNYTIVIVTHNMQQAVRISDKTAFFL
                     MGEVVEYNKTSQLFSLPQDERTENYITGRFG"
     gene            complement(6976..7854)
                     /gene="pstA"
                     /locus_tag="ACUBV3_000733"
     CDS             complement(6976..7854)
                     /gene="pstA"
                     /locus_tag="ACUBV3_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HHU3847255.1"
                     /translation="MENINQRTSKQYLASLKKDPLSFFLFAIVYLGAILSFATIAFVV
                     IYILVKGLPHVNTGLFAWTYNTQNVSLLPAFINTIFIIALTLLFAVPLGIGGSIYLTE
                     YARRDNPYLKIIRVATETLAGIPSIIYGLFGALFFVKYTHLGLSLISGSLTLSIMILP
                     LIMRTTEEALLSVPDSYREGAFALGAGKLRTIFKIVLPSAMSGIFAGIILAVGRIIGE
                     SAALIFTAGTVAKVAHSVFSSSRTLAVHMYAISGEGLYVDQTYATAVILLLLVIIVNF
                     VSGLVAKRLGSESNSE"
     gene            complement(7856..8701)
                     /gene="pstC"
                     /locus_tag="ACUBV3_000734"
     CDS             complement(7856..8701)
                     /gene="pstC"
                     /locus_tag="ACUBV3_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HHU3847256.1"
                     /translation="MKQTKEGLMQAVFFITACVSVISAILICLFLFSSGLPAITKIGW
                     GNFIFGKVWHPSNNIFGIFPMIVGSLYVTAGALLLGGPIGILTAVFMAYFCPENIYKP
                     LKSAINLMAGIPSVVYGFFGLVVIVPMIRQYIGGFGMGVLAASILLGIMILPTIVSIS
                     ESSLRAVPESYYEGGIALGASHERSVFFAVLPAAKRGILASVVLGIGRAIGETMAVIM
                     VAGNQAVLPQSLTSGVRTLTTNIVMEMGYSSGLHRQALIGTAVVLFIFILMINISFSA
                     LQKEE"
     gene            complement(8716..9597)
                     /locus_tag="ACUBV3_000735"
     CDS             complement(8716..9597)
                     /locus_tag="ACUBV3_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000716337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HHU3847257.1"
                     /translation="MKKHKMLSLLAVSGLMGIGILAGCSNDSSSSSKGTINIVSREEG
                     SGTRGAFIELFGIESKNKKGEKVDHTSDAATVTNSTSVMLTTVSKDPSAIGYSSLGSL
                     NSSVKVLKIDGKNATVKDIKSGSYKISRPFNIVTKEGKEKEATKDFIDYILSKDGQAV
                     VEKNGYIPLDNAKAYQAKVSSGKVVIAGSSSVTPVMEKIKEAYHKVNAKVDVEIQQSD
                     SSTGITSAIDGSADIGMASRELDKTESSKGVKATVIATDGIAVVVNKKNKVNDLSTKQ
                     VKDIFTGKTTSWSDLSK"
     gene            complement(9798..10385)
                     /locus_tag="ACUBV3_000736"
     CDS             complement(9798..10385)
                     /locus_tag="ACUBV3_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HHU3847258.1"
                     /translation="MIIAIQGGMAVGKTTALKIIENVYPKIAISFEDISPVVDKINKH
                     QLDNNKLDDYCEIQQLFIQHERDRYELVRDNEIVLMDYGAEEIAFYTQYFPKVAGYSW
                     TLPSNLLLEVNRLKECFADYIIFLDADETVLYQRQKNDFNRQRHFFKWYTHHLLPAKK
                     HYLDTFSNVIHINTNHLNAEEVAEKIIDIIRKLTL"
     gene            complement(10382..11122)
                     /locus_tag="ACUBV3_000737"
     CDS             complement(10382..11122)
                     /locus_tag="ACUBV3_000737"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001188249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HHU3847259.1"
                     /translation="MQQYFVNGEAGAYVTIEDKDTIKHMFNVMRLTEDDQVVLVFDDA
                     IKRLAKVVDSSAHRFQILEELDNNIEMPVQVTIASGFPKGDKLDFVTQKATELGAAAI
                     WGFPADWSVVKWDGKKLAKKEDKLAKIALGAAEQSKRNRLPQVRLFEKKADFQAELAG
                     FDKIFIAYEESAKEGELSALAQNLQTVKAGDKLLFIFGPEGGISPKEIAAFEEVGAIK
                     VGLGPRIMRTETAPLYALSVISYSAELL"
     gene            complement(11122..12075)
                     /gene="prmA"
                     /locus_tag="ACUBV3_000738"
     CDS             complement(11122..12075)
                     /gene="prmA"
                     /locus_tag="ACUBV3_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011225343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HHU3847260.1"
                     /translation="MNTWNELTVHVNREAEEAVSNLLIETGSQGVAISDSADYLGQED
                     RFGELYPEVEQSDMIAITAYYPDTLDIEAVKADLADRLANFEGFGLATGSVNLDSQEL
                     VEEDWADNWKKYYEPARITHDLTIVPSWTDYEAKAGEKIIKMDPGMAFGTGTHPTTKM
                     SLFALEQVLRGGETVIDVGTGSGVLSIASSLLGAKDIYAYDLDDVAVRVAQENIDMNP
                     GTENIHVAAGDLLKGVQQEVDVIVANILADILIHLTDDAYRLVKDEGYLIMSGIISEK
                     WDMVRESAEKAGFFLETHMVQGEWNACVFKKTDDISGVIGG"
     gene            complement(12072..12536)
                     /locus_tag="ACUBV3_000739"
     CDS             complement(12072..12536)
                     /locus_tag="ACUBV3_000739"
                     /inference="COORDINATES: protein motif:HMM:NF020451.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MepB family protein"
                     /protein_id="HHU3847261.1"
                     /translation="MVIEIVELLREWYSSFEVKEEKWNEKYEGILIRIEGDGNYKRCR
                     LAHKTASKMGYFTVFWQKDGNGKNIPFYEQDEVEDLIIVIKDGRWKGLFIIPKEVAVS
                     KGILSSANSQGKMAMRFYPPWCSDLNRTALVTQRWQLNYFIDLSRNNEGVTT"
     gene            12654..13370
                     /locus_tag="ACUBV3_000740"
     CDS             12654..13370
                     /locus_tag="ACUBV3_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HHU3847262.1"
                     /translation="MKTVKEVSILSGVSVRTLHHYDKIGLFPPTALSEAGYRLYDDEA
                     LIRLQEILLFRELEFPLKDIKYLLEQAKEERQDLLAQQIKLLEWKRSHLEQVITHAKR
                     LQEKGDDYMNFDVYNKTELEQLQAEAKEKWGQTAAYKEFAQKHASDDFAQISQEMAKI
                     MVQFGQLKTQNVSDESVQMCVKRLQDYISQHFYTCTNEILAGLGQMYQSDDRFSQSID
                     KAGGAGTSEFVSQAIAYYCQ"
     gene            complement(13409..13879)
                     /locus_tag="ACUBV3_000741"
     CDS             complement(13409..13879)
                     /locus_tag="ACUBV3_000741"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HHU3847263.1"
                     /translation="MITLQKAEASDLEKIIAIQRASFKAVYEKYHDQYDPYVEEVEQI
                     RWKLVERPDCFYHFVLVDETIVGFLRLVIKDEEKRAWLGTAAILPQYQGQGYGSAVMA
                     LLEKTYPKLTKWDLCTIAQEKLMVSFYEKCGYHSTHTEPEQEGMDMVYMTKKIE"
     gene            complement(13851..14309)
                     /locus_tag="ACUBV3_000742"
     CDS             complement(13851..14309)
                     /locus_tag="ACUBV3_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HHU3847264.1"
                     /translation="MDIFDELFDFSGAKIALFCEGKILTSLRDDFPDLPYAGFWDLPG
                     GGREDNETPLECLFREVDEELSLTLTRNHIDWVKTYRGMLKPDKLSVFMVGHISQKEY
                     DSIVLGDEGQDYKLMSIDEFLSHKKVIPQLQERLRDYLEVEDDYFTKGGS"
     gene            complement(14296..14439)
                     /locus_tag="ACUBV3_000743"
                     /pseudo
     CDS             complement(14296..14439)
                     /locus_tag="ACUBV3_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001868493.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
     gene            complement(14445..14915)
                     /locus_tag="ACUBV3_000744"
     CDS             complement(14445..14915)
                     /locus_tag="ACUBV3_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HHU3847265.1"
                     /translation="MAKFGFLSVLEEELDKHLQYDFAMDWDKKNHTVEVTFILEAQNS
                     SAIETVDDQGETSSEDIVFEDYVLFYNPVKSRFDAEDYLVTIPYEPKKGLSREFLAYF
                     AETLNEVATEGLSDLMDFLTDDSIEEFGLSWDTDAFENGRAELKETEFYPYPRY"
     gene            15242..15733
                     /locus_tag="ACUBV3_000745"
     CDS             15242..15733
                     /locus_tag="ACUBV3_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001879696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HHU3847266.1"
                     /translation="MIIRPMQDCDREAVLTLQNTGWTALTSPVYDRKWTESDLEKNLA
                     NGMSFFVAEVDDKIAGVLDFGPYYPFPAGKHVATFGILIAEPYQGQGLGKALLKALLT
                     EAKAQGYIKIAMHVMGNNSRAISLYQKYGFTEEARITKAFFIENHYVDALIFAKDLEN
                     NHG"
     gene            15726..16994
                     /locus_tag="ACUBV3_000746"
     CDS             15726..16994
                     /locus_tag="ACUBV3_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HHU3847267.1"
                     /translation="MADNLALRMRPRNINEVIGQQHLVGNGKIIDRMVAANMLSSMIL
                     YGPPGIGKTSIASAIAGTTKYAFRTFNATVDSKKRLQEIAEEAKFSGGLVLLLDEIHR
                     LDKTKQDFLLPLLENGNIIMIGATTENPFFSVTPAIRSRVQIFELEPLSNEDIKKAIQ
                     LAISDKERGFPFLVTIDDEALDFIVTATNGDLRSAYNSLDLAVMSTSPNEDGSRHISL
                     ETMENSLQRSYITMDKNGDGHYDILSALQKSIRGSDVNASLHYAARLVEAGDLPSLAR
                     RLTIIAYEDIGLANPEAQIHTVTALEAAQRIGFPEARILIANIVVDLALSPKSNSAYL
                     AMDAALADLRRSGNLPIPRHLRDGHYSGSKTLGNARDYKYPHAYPEKWVKQQYLPDKL
                     VGHNYFEANETGKYERALGSNKERIDKLSD"
     gene            17083..17275
                     /gene="ssrS"
                     /locus_tag="ACUBV3_000747"
     ncRNA           17083..17275
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACUBV3_000747"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            17276..17348
                     /locus_tag="ACUBV3_000748"
     tRNA            17276..17348
                     /locus_tag="ACUBV3_000748"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17309..17311,aa:Lys,seq:ctt)
     gene            complement(17449..18570)
                     /locus_tag="ACUBV3_000749"
     CDS             complement(17449..18570)
                     /locus_tag="ACUBV3_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018366757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HHU3847268.1"
                     /translation="MATYRQRGKKKLWDYRIFNEKSELVASGSGFKTKREAMNEAMRI
                     EQQKLLVNSISSDITLYDLWFEWYSLIIKPSNLAETTKNKYFTRGSVIRKLFGNQKVN
                     KIKHSAYQRKLNMYAEKYTKNHVRRLNSDIKKAIQFAKRDGVLLSDFTDGVVIAGRKF
                     VKDADDKYLHSISDYKKVISYLENNLDYSNSIVYYLLLVLFKTGLRVGEALALTWDDV
                     NFEDLEIKTYRRFSGDKGTFSPPKTKTSIRTIPISQSLALTLRRLKDDQQVMLKNLKI
                     VNINNQIFYDYRYGVSSNSAINKSLRNVLHLLNIDSKMTAIGARHTYGSYLLAKGVDI
                     WVVARLMGHKDITQLLETYGHVLTEVINKEYETVRSLVS"
     gene            complement(18620..18946)
                     /locus_tag="ACUBV3_000750"
     CDS             complement(18620..18946)
                     /locus_tag="ACUBV3_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001188032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HHU3847269.1"
                     /translation="MQQVFNVSVPVPDDVVIISKEEYLNLLSDNEQGKWWDIDNLQEL
                     LGIGRSKLINDILLNPDIKTEVDLSINPNGFIVYPKGKGSRYKILATKARKYFEDNFG
                     SILLNS"
     gene            complement(19139..19771)
                     /locus_tag="ACUBV3_000751"
     CDS             complement(19139..19771)
                     /locus_tag="ACUBV3_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000578746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3847270.1"
                     /translation="MIGDYSVMDWATLGGVVGIIFTNVGVLISNSRDNKAIQRELELL
                     EEGQEKLVDEFSKISTNQYDKYVLIQSNLSNNIEKNKQELVQKNSYVKEDTKYIRDEM
                     LIEKKSREEVYNHVKNGDKLIEKMAFANELILKFGEVSRENQMLGLKVNSLEEKIVDL
                     SNQPKNDEISKLRKSISSFERELSRFEDGGYSEAEEIKSTLRRILNDLEG"
BASE COUNT         6208 a         4212 c         3125 g         6283 t
ORIGIN      
        1 atacagactt tatctgaacc taagggtatc ttttattata agatattaga aatccatggt
       61 aatttccaag ccgtaatgat cactaacgac aggtgcttca ccaccttcaa aaactacttg
      121 acttgaactg attgaaaggt cttcgctagt aaaaatatgg tcaactttaa gagccttttt
      181 attcccttcc caaccatcaa tatcagccat aatagtatgg tccccaaata catgattggc
      241 tatttggtgg ctatccttta atgctaatgg actattaaga acaagttcat aaccctgatt
      301 tccagtagga ttattaaagt cacccatgag taaaagtgga gtttctacct caagtagggt
      361 agtttctagt cgtttccact cctccgcaaa tcctttctca aaccatgata aatggaggct
      421 aactactgta acgactttgt cattcagagt ggtctcagct accagagcac gacgagtatg
      481 ataatcagtc tcatcgtcta cagctgaaac taaaatatcc tctggcttta atggtttcag
      541 agaaagtatc gctacccctt catggtactt actataacca atatgattat aagcccatgt
      601 ccaatgatag tgtagacctt gaagatttag gtaatgcact aattgcaagg caaagttatc
      661 ttgatgaaga gctggtgtac cttcaatcgg atgataattt ggactactct tagcaagagg
      721 ggaagatatg ctctggttca cctcttgtag gcagatgata tcatattttt cacggaagat
      781 atgctctgct aaatcaaata atttctttag agcatttact tccatccatg aatgagtatt
      841 taacgttaag cattttgcca tagagtatcc tttcatcaga aagaagctga gacttgccca
      901 gctcctatcg tttgttacag ttcgactttt gccacaactg ttttagctat ttgtggacct
      961 gatttttcaa gtttaacagt ttttatatct gaaacatttg taaagacgat aatcacactt
     1021 gtctctttac cagctgcttt gatagcttcc aaatctgcta ctacagcaag atcgcctgct
     1081 accacttttt gaccactaga aattttaacc tcaaatggtt gaccatcaag agctactgta
     1141 tctagaccga tatgtactaa aacttctagg ccactttctg ttaataaacc aaaagcatgt
     1201 tttgttggaa agacactagt aactgtacct gaaactggaa cataaatatt gccatcagtt
     1261 ggttccacag caaagccatc acccatcatt ttttgagaaa agacagggtc tttaacaaca
     1321 tccaatgctt ctacttgacc attagccacc gaaacaattt cttctgttat tcctttataa
     1381 gttgtaactg gggctgctgc tacaggttga cctgtttcta aatcaactat tggaataact
     1441 gtacctgaat ctagcaaatc ttgaatgtct gatttaagga catctgcttt aggtccgtaa
     1501 atagcttgaa cgccattacc ttttaacacc aatcccattg caccagcttt tttccaagct
     1561 gcttcagaac ccacgctatt tccatcttta actgtgacac gaagacgagt catacatgca
     1621 tctacatctt caatgttatc acgaccacca agtaaattaa taacttgtac aacttgagaa
     1681 tttgcatcag cagaaccaga agctgttgaa ggagcattat caacctcttc gttatcataa
     1741 ttaccattac gtcctgctgt tgctaaattg aattttttaa tcataaagtt tgcaataaag
     1801 tacatcaata ctgcaaaaag aagtgtcacc caaacaaagt tgaaaatatc accaccaagg
     1861 cccgctttta tgcccatagg aacacgtgtt aaaaattcta tattaccaaa tgaatgaacg
     1921 cggaggttaa caatatctgc catagcaaac gcacatcctt gaacgacagc atatacaagg
     1981 tagagtggca ttgctgcaaa catgaacatg tactcaattg gctcagtaac acctgtcaag
     2041 aaagtagccg ctgctgctga aataaacatt gacttgtatt tcaatttttt atctttatca
     2101 acgttacgat acatagcata agataatccc atcaaaatac ctgaagcacc aatcatttgc
     2161 cccactttaa aacgtgctgg agtgacactt gtcaataagt gatggtattg acttgaattt
     2221 gagcctttta agttaattaa atcaccaacc catgctaacc acagtggatc ttgtcccaac
     2281 acctgtgctc ctttagttgc acctgtaagg acagtgtatg tgccacctag ttgcgtgtag
     2341 ttcattggaa tagttaacat atggtgtaaa ccaaatggga gcaataaacg ctctaacgta
     2401 ccgtagacaa atggtgctaa tattggagca ctgtcttgtg aacttgcaat ccatttacca
     2461 aagccattga tacctgattg aacaaccggc caaaatactg ataaaattaa tgcaacaatg
     2521 actgaacgat aaataacaac aaatggaaca aaacgttttc cattgaaaaa agttaaagca
     2581 tctggtaatt tacgatagtt ataatattta ttaaaggcag ttgcaccaac gaaacctgaa
     2641 ataataccga caaatacacc catattaaga gcaggttgcc caagaacgtt aacaaagtaa
     2701 tctgaaactc tgatggttgc accaaatacc gtttgaacag ttgctttact gtctgctaac
     2761 atatctgttt taacaccaaa aaagttccct gtaataaggt taataagaat aaaggataag
     2821 ccagccgcaa aagcaccacc ggcacgttct ttagcccagc taccaccaat agccaatgca
     2881 aataagatgt gtaagttacc aataactccc caaccaattt gagcaataac attagctatg
     2941 cgccctaata aaacatttga tggatccaat agactaatgg agttaccaat actaaccatc
     3001 aatcctgctg ctggcataac agcaataaca accatcaaag ccttcccaaa cttctgccaa
     3061 aattcaaaac taaagagctg tttgacatta tttttcatct tcaatctcct tttatgcact
     3121 taatagtgca atcgtttgcg ttattatggt tttattatag cacttatttg ttttttgtaa
     3181 acccttactt atatattttt tctgaattac taaaaaagtc tagataatat catctagact
     3241 tttatttgtg agtttttatt gtttgtgaag taaaacggtc attgttgttc cctgccccaa
     3301 atggctgtcc acttttatac taccattgtg aaaatctaaa gcactcttta caatagacaa
     3361 acctaaacca gtccccccaa ctatttttga gcgactttta tcgacgcgat agaaacgttc
     3421 gaaaatacgt tttttgtcct tctctgcaat gcctagtccc gtatcactaa cttctaaggt
     3481 aattgtatca ggtgaatttt tcaaagtcac atttacttga cctttctcat ggttataggt
     3541 gatagcattg tcacacaagt tgtacaccaa ggaattcaat aaaactggat tccctcgcaa
     3601 aatagcttct tctccattaa aattgatttg tatgtgcttc ttatctgctt tagcttgtaa
     3661 accttctaaa actttttgtg ttaagtcata cagatttact gtctcttgcg gtaatttttc
     3721 ttgttcatca aggtgagaaa ggttgataat atcctcaacc aatttaacta atcgttcaga
     3781 ttctttatga attttagctg caaattgtgg gacttcttcg tttggaacca tttgattagc
     3841 taacagttct gagtaaccag aaataacatg aagcggggtt ttcaattcat gagagacatt
     3901 ggcagtaaat tcacgttgaa gttgttccat ttggagttga tcagtcacat caaacagtag
     3961 aacaaccaat cctgttactc ggttgttatc tgtagttggt cttaccaaga cgcggtaatg
     4021 attattttca atgccaatat tagcctcttt ctttttccct tttagtcctt gatcgattaa
     4081 gtctttgaga gttaaatctc ggctaacttc catcataaag cgcccatgcc aatcatcatt
     4141 aatttggaat aactttaaag cttctgcgtt gatactaacg attcttgctt gatcatctaa
     4201 aagaatcatt ccttccttaa ttttagaaat aatggtatca aattcttttt gacgcttttg
     4261 taataacagt ttttgatgtt gaatcttagc ctgatggcta tcaagacgcc ttaataatgg
     4321 tgttatctcc tcgtaactat catttgataa aggatgatct aagtcaatat tgtttaaagg
     4381 ctctgatact ttttttgcta tatatcgtgc aattaataca gataaaataa tagcaagtaa
     4441 gatgataatt gctaagggac ttatcatacc aaggagaaga taaaaaatag tttgttgcgc
     4501 aactgataaa cgaactatcg ttccattatt taaacgttgc gcagcataga tagacttctc
     4561 agtaagtgtc gctgaccacc tcgtgctctc accataacca ctcttgatag cttctttaat
     4621 ttcttgacga ttggcgtgat ttttcatatg ttttgcatct gattgggtat cataaagcac
     4681 ttgtccctta ttatctaccc aagtaatacg cacatttgaa gttttaagat tttcaaaata
     4741 atctttacct tcaaaggaaa ttccttgtga cgccagtgcc gtttgtgttc tcaactgttc
     4801 tcgctgaatg tgattgaagt aattatagag aaatcccata atcatcaaaa ttgtaactaa
     4861 aacaattccc aaactagcag ataaagttgt acggaaaatt tttttagtca tgtctttcct
     4921 ccaaacggta tccaactccc cttactgtag caatcaaata gccatcctca ccaagttttg
     4981 ttcgcagtgt cccaatgtga acatccacag ttctagtttc tcctaaaaat tgttctcccc
     5041 aaaccttatc cagaagttct tgccttgtaa agactctatt gggattacgg aagaataata
     5101 ctaataactc aaattccttt agggttaagt ggattttttc cgttcccctt ttgacccaat
     5161 aattagtcgg atcaatctct aaatttccaa tgataatatg agcttttgaa tctacttggc
     5221 ggctacgtct caagactgct tttattcgtg aaatcatttc catcatccca aaaggtttga
     5281 cgagataatc atctgctcca agatctaacc ctttaacttt atcatactca ctacctttag
     5341 cagttgtcat gattactggt atcatttccg tttgatgttt tctccttaaa cgctctaaaa
     5401 tagtcaaacc atcatctccc ggcaacataa tatcaagcaa aatcaagtct ggaactttct
     5461 cctgaatagc ttcccaaaaa agctctgaac tcgaaaatcc ttgagcttta aaacctgcca
     5521 tttgcaaagt atagagcatc atttcgcgaa tatcagcatc atcttcaaca caatatatca
     5581 ttacgcttct ccatctaggt tacctgttat tgagaaaagc acccacttag caatatttac
     5641 tgtatgatca ccaatacgct ctaggtactt ggcgatcatt aaaacatcta tgggatattc
     5701 accatcaaca tcctgtaccg aaaaatataa cactaactgt ttctttattg tatcaaattc
     5761 ctggttaact gtcctatctt gttcaattac ttgatgcgct tgttcttcat tgtcataaat
     5821 aaaagcatct atactcttag tcagcatact aatgacatta ttagacatgc tgttaagttg
     5881 atccctttca gaacctgcac cttggataat atgacctaaa ctaacaattt ccgcaatttc
     5941 ataagcttga gcaccaatac gtttcatatc ataaaccatt tttaatgcag aagaaatacg
     6001 gcgtaaatcc ttggcaactg gttgctgacg gagtaaaagt tttaaacatc tctcctcaat
     6061 atcccgttcc atttgttcaa tcttgtgata agtttcagaa atatcgtcaa ggtaaacgtc
     6121 attactattt gttagagcac ctaaggactt cccaatgatg tcctcacaca atgcccccat
     6181 aaaaataatt tctttattaa ggtcattaag ttgacttgca aatcgtgaac gaatcatccg
     6241 aatctccctg taatgtagtt ttctgtacgc tcatcttgtg gcaatgaaaa gagttggctc
     6301 gttttgttat attcaaccac ttctcccatc agaaagaatg ctgttttatc agaaatacga
     6361 acagcttgtt gcatattatg tgtcactata acaatagtgt agttcttctt cagttggata
     6421 accaattctt caatttttgc tgttgaaata ggatccaagg cacttgtggg ttcatccata
     6481 agtaaaacat caggctcaat agcaagagca cgcgcaatac acaaacgttg ctgctgtccg
     6541 ccagacatac ccagagcaga tttgtgaaga cgatctttaa cctcatccca tagagccgcc
     6601 tgtttaaggg aacgttcaac gatatcatct aactctgctt tggaatgaat accgtgcgtt
     6661 cttggaccaa aagcaacatt atcataaatg ctcattggaa acggattagg tttttgaaac
     6721 accattccaa cctttttacg caattggtta atatccaatt gtctatagac atcctcgcct
     6781 tctaaagtaa tatcccctgt gattttgcaa tttttgacca aatcattcat acggttaata
     6841 cttttcaaga gagtagactt tccgcaccca gaaggtccaa taaaggctgt gatttctttt
     6901 tcttcaatgt ctaaattaac atccttcaga gcgtgaaatt ctccataata caaatctaaa
     6961 ttattaatta ctaatttact cactgttcga ctcacttcct aatcgtttgg ctactagtcc
     7021 agatacaaaa ttgacaataa taactaaaag caataagatg accgcagtcg cataagtttg
     7081 gtcaacatat aagccctcac ccgaaatagc atacatatgt actgctaaag tacgagatga
     7141 actaaatacc gaatgtgcta cttttgcaac tgttccagct gtgaaaataa gtgctgctga
     7201 ctcaccaatg atacgtccta ccgctaaaat aataccagca aaaataccag acatagcact
     7261 aggtaatacg attttaaata tggttctcaa tttaccagca ccaagcgcaa aggctccttc
     7321 tcgataagaa tccggaacag atagtaaagc ttcttcagtt gtcctcataa tcaaaggtaa
     7381 aatcatgatg ctcaaagtca gagaacccga tatcaaagat aagcctaagt gtgtgtattt
     7441 tacaaaaaat aatgcaccaa acaaaccata aataattgaa ggtatccctg ccaatgtctc
     7501 ggttgcaaca cggataattt tgagataagg attatcacgt ctagcatatt ccgttagata
     7561 aattgatcca ccaataccaa gtggaactgc aaataataaa gttaaggcga tgataaagat
     7621 agtgttaatg aaagctggta atagtgaaac gttctgagta ttataagtcc atgcaaacaa
     7681 gccagtattc acatgaggaa gccccttaac taaaatataa ataacaacaa atgcaattgt
     7741 agcaaaactt agaattgctc ccaaatagac aatagcaaaa agaaagaagg aaaggggatc
     7801 tttttttaaa gatgctaaat attgttttga cgttctttgg ttaatatttt ccatcttatt
     7861 cctctttctg taatgctgaa aaactgatat taatcattaa tataaagata aagagtacca
     7921 cagctgtacc aattaaggct tgacgatgca aaccagaaga atagcccatt tccataacaa
     7981 tgttagtggt tagagttcta acgcctgatg tcagcgattg aggcaagaca gcttgattac
     8041 ccgctaccat gatgacagcc attgtttcac caatagcacg tccaattccc aaaacaaccg
     8101 atgctaaaat gccacgttta gcagctggta aaacagcaaa gaagacactt cgttcatgag
     8161 aggcaccaag ggcaataccg ccttcataat agctctctgg aacagctctt aaagaagatt
     8221 ctgaaatgct aacaattgtt ggtaaaatca taattcctag caaaattgaa gctgctaaga
     8281 ctcccatacc aaagccacca atatattgcc ttatcatagg gacaataaca acgagtccaa
     8341 agaaaccata aacaactgaa gggattcccg ccattaaatt gatagctgac tttaaaggct
     8401 tataaatatt ttctgggcag aaataagcca taaaaacagc agtaaggata cctatcggtc
     8461 ctcctaaaag taaagctccc gctgtaacat ataaggaacc tacaatcatt ggaaagatgc
     8521 caaaaatatt attactagga tgccagactt taccaaatat aaaatttccc cagccgattt
     8581 tcgtaatagc tggaagacca ctagagaaga gaaagaggca aattaaaata gctgatataa
     8641 cactgacgca agccgtgatg aaaaacacag cttgcataag accttctttt gtttgtttca
     8701 ttgtttactc ttaccttact ttgataagtc agaccaagac gttgtctttc cagtaaaaat
     8761 gtcttttact tgttttgttg atagatcatt aactttattt ttcttattaa caactactgc
     8821 aataccatct gttgcaataa cagtagcttt gacccctttg gatgactctg tcttatccaa
     8881 ttcacgtgat gccataccta tatctgctga tccatcaatt gcgcttgtaa tcccagttga
     8941 agaatcactt tgttgaattt caacatctac ttttgcattt actttatgat aggcttcttt
     9001 aatcttttcc ataaccggcg ttactgaact tgaacctgct ataacaactt taccagatga
     9061 cacttttgct tgatatgctt tagcattgtc aagtggaata tagccatttt tctcaacgac
     9121 tgcttgccca tctttgctta agatataatc aataaaatct tttgttgctt ctttttcctt
     9181 accttctttt gttacaatat tgaaaggacg agaaatctta tagcttcctg atttaatatc
     9241 ttttacagtc gcatttttgc catcaatttt aagcacttta acgctactat ttaatgaacc
     9301 tagtgaagaa taaccaatag ctgacgggtc tttagagaca gtggttagca taactgaagt
     9361 tgaattggta acagttgctg catcagaagt atggtctact ttttcgccct ttttattttt
     9421 tgactcgatc ccaaataact ctatgaaagc tccacgagtt ccagaccctt cctctctaga
     9481 aacaatgtta attgtccctt tagatgaaga tgatgaatca tttgagcatc ctgctaggat
     9541 gccaattccc ataagaccag ataccgccaa aagagatagc attttgtgtt ttttcatgat
     9601 gaactcctaa atattttttg atttgatttt attatagtta agaaatatca aatctctttg
     9661 aggatttttg taaaatcaat gtaaaattaa aaagagttgg aaaattatcc aaactccttt
     9721 tttctattat ggtaattcag ccagtaagtc ggattgaaac agttgttaca tcacataatt
     9781 ggagtataac tatcccatta tagagttaac ttacgaatga tgtcaatgat tttttcagcc
     9841 acttcctcag catttaaatg atttgtatta atatgaataa cattggaaaa tgtgtctaaa
     9901 taatgttttt tagcaggcag caggtgatga gtataccatt tgaaaaaatg acgctgtcta
     9961 ttaaaatcat ttttttgacg ttggtatagg accgtctcat ctgcatctag aaaaataata
    10021 taatcagcaa aacattcctt tagcctattt acctctagta acaaattaga aggtaaagtc
    10081 caactataac ccgccacctt tggaaagtat tgtgtataaa aagcaatttc ctcagctcca
    10141 taatccatta acactatttc attatctcta acaagttcgt atctatctcg ctcatgctga
    10201 ataaatagtt gttgaatttc acaataatcg tctaatttat tgttatcaag ctgatgttta
    10261 ttaatcttat caactactgg agaaatatcc tcaaaactaa tagcgatttt ggggtaaaca
    10321 ttctcaatga ttttcaaagc tgtggttttc cctacagcca taccaccttg aatagcaata
    10381 atcataataa ctccgctgag taactaataa cacttagagc atagagcggc gctgtttctg
    10441 tgcgcatgat gcgtggcccc aagcccacct tgatagctcc aacctcttca aaagctgcga
    10501 tttcttttgg agaaatgccc ccttctggtc caaagatgaa gaggagcttg tcacctgctt
    10561 ttacagtctg caagttctgg gctagcgctg acaattcacc ctccttggca gactcttcat
    10621 aggcgataaa aatcttatca aagcctgcaa gctccgcttg aaaatcagcc ttcttctcaa
    10681 ataaacgaac ctgaggaaga cgattacgct tgctctgctc cgctgcgccc agagcaattt
    10741 ttgccagctt atcttctttt ttagctagtt tcttgccgtc ccacttgacg actgaccagt
    10801 cagctgggaa tccccagata gccgcagcac ccaattctgt tgccttttgc gtcacaaaat
    10861 ccaacttgtc tcccttggga aaacctgagg caatggtcac ttgaactggc atttcaatat
    10921 tgttgtctag ctcttctaga atttggaagc gatgagcaga gctgtccacc acctttgcca
    10981 gacgcttgat ggcatcatca aaaaccaaga ccacttggtc atcttctgtc aatcgcatga
    11041 cattgaacat gtgcttgatg gtatccttgt cctcgatggt cacataggca cctgcttcgc
    11101 catttacaaa atactgttgc attagccgcc aatcactcct gaaatatcat ctgtcttttt
    11161 gaaaacgcag gcattccact caccttgaac catgtgagtc tcaaggaaga aaccagcttt
    11221 ttcagcactc tcacgcacca tgtcccactt ctcagaaatg ataccagaca tgatgagata
    11281 gccctcatcc ttgaccaaac ggtaggcgtc gtctgtcaga tggatcaaaa tatccgccaa
    11341 aatattggca acaataacat ctacctcttg ctggacacct ttgagcagat caccagcagc
    11401 cacatgaata ttttcagtgc cagggttcat atcgatattt tcctgagcca cgcgcactgc
    11461 aacatcatcc aaatcatagg catagatgtc cttggcaccc aagagagagc tagcaataga
    11521 caaaacgcca gaaccagtcc ccacatcgat aaccgtctca ccaccacgaa gcacttgctc
    11581 caaggcaaaa agactcatct tagtcgttgg gtgagtccca gtcccaaaag ccataccagg
    11641 atccatcttg ataatcttct caccagcctt agcctcgtag tccgtccatg acggcacgat
    11701 ggtcaaatca tgggtaatgc gagctggctc atagtacttc ttccagttat cagcccagtc
    11761 ctcttcgacc aattcctgac tgtcaaggtt gacgctaccc gtcgcaagac caaagccttc
    11821 aaaattagct aaacgatcag ccaaatcagc cttaaccgcc tcaatatcaa gcgtatcagg
    11881 ataataagca gtaatagcaa tcatatcaga ctgctccacc tcaggataaa gctcaccgaa
    11941 acgatcctcc tgtccaaggt agtcagccga atcgctaatg gcaaccccct gactgccagt
    12001 ttcaatcaat aaattcgaga cagcttcctc tgcctcacga ttcacgtgaa ccgttagttc
    12061 attccatgtg ttcatgtagt aactccttca ttatttctac ttaaatcaat aaaatagttc
    12121 aactgccatc gctgtgtcac caaagctgtt ctatttaaat cgctacacca aggagggtaa
    12181 aatcgcatag ccatctttcc ttgagagtta gcagatgaaa gaataccctt actcactgca
    12241 acctctttgg gaatgatgaa caaacctttc cacctaccat ctttaatgac aataatcaaa
    12301 tcttccactt catcttgttc ataaaaagga atatttttac catttccatc tttctgccaa
    12361 aatactgtaa aataccccat cttgctagct gtcttatgag ctaaacgaca ccttttatag
    12421 tttccatcgc cttcaattct tatgaggata ccttcatatt tttcattcca cttttcttct
    12481 ttaacttcaa aggaagaata ccattcccta agtagctcaa ctatttctat aactatccct
    12541 cctttatctt caaatcaata tgattaacca tacttttcaa aataatttat tttttgcttg
    12601 accctgacct taggttatcc tttatactat gtaagtgaga ggaggttatt accatgaaaa
    12661 cagttaaaga agtcagtatc ttaagcggtg taagtgtgcg caccttgcat cactatgata
    12721 aaatcggttt attcccacct accgccctat ccgaagcagg ctatcgactt tatgacgatg
    12781 aagcattgat tcgcctgcaa gaaattttgc tatttcgtga gttggaattc cctctcaaag
    12841 atatcaaata cctgcttgag caagcaaaag aggaaaggca agacttatta gcccaacaaa
    12901 tcaaattgct tgaatggaaa agatcgcacc ttgagcaggt tatcacccat gcgaaacgat
    12961 tacaagaaaa aggagatgat tatatgaatt tcgatgttta taataaaaca gaattagaac
    13021 aattacaagc tgaagcaaaa gaaaagtggg gacaaacagc agcctacaaa gaatttgctc
    13081 aaaaacatgc ttcagatgac tttgcacaaa ttagccaaga aatggcaaaa ataatggtac
    13141 aatttgggca attaaagaca caaaatgtat ctgatgaatc tgttcaaatg tgtgtgaaac
    13201 ggctacaaga ctatatctct cagcattttt acacctgtac caatgaaatc cttgctggac
    13261 taggacaaat gtatcaatca gatgaccggt ttagtcaatc aattgacaaa gctggtggag
    13321 caggaacaag tgaatttgtc agccaagcta ttgcttacta ttgccaataa actatcagaa
    13381 aactagacaa tcatctagtt ttcttttttt attctatctt ctttgtcata tagaccatat
    13441 ccattccttc ttgctcaggt tcggtatggg ttgaatgata accacatttt tcataaaaac
    13501 tgaccattag tttttcttgc gcaatcgtac ataaatccca ctttgtaagc ttaggatagg
    13561 ttttctctaa taaagccatc actgctgagc cgtagccctg cccttgatat tggggaagaa
    13621 tagccgccgt accaagccaa gcacgcttct cctcgtcttt gataaccaat ctaagaaaac
    13681 caacaattgt ctcatcaaca aggacaaaat gatagaaaca atctggtcgc tccactagtt
    13741 tccatcgaat ctgctcaact tcttccacat agggatcgta ttgatcgtgg tatttctcat
    13801 agactgcctt aaagctagct ctctgaatgg caataatctt ctctaaatca ctagcttccg
    13861 ccttttgtaa agtaatcatc ttccacctcc aaataatctc tcaacctctc ctgcaactga
    13921 ggaatgacct ttttatggct taaaaattcg tcaatggaca tgagtttgta gtcttgtcct
    13981 tcatcgccta gaacaatgct atcgtactct ttctgactga tatgtccaac cataaaaact
    14041 gataatttat caggcttcaa cataccccta taagtcttga cccaatcaat atggtttcga
    14101 gttaaggtca gacttaattc ttcatcaact tcacgaaaaa gacattccaa aggtgtttca
    14161 ttgtcctcac gaccaccgcc tggtaagtcc caaaagcctg catagggcaa atctggaaaa
    14221 tcatctctta ggctggtcaa aatctttccc tcgcagaaaa gagcgatctt tgcacctgag
    14281 aaatcaaata actcatcaaa aatatccatg caataacctc aattctattc ctaaagctcc
    14341 atattttctg atttgttcag gggcataata acgttccata tcctctggtc tggctgtttc
    14401 tatatcatcc aatgtatatc cacattttag caaatccatc aagtctaata tctcggataa
    14461 gggtaaaact ctgtctcttt cagttctgct ctgccatttt caaaagcatc cgtatcccaa
    14521 gaaagaccga actcttcgat actgtcatcg gtgaggaagt ccatgaggtc gctcaaccct
    14581 tcagtggcaa cctcgttgag agtctccgca aaataagcca gaaactcacg cgacaagccc
    14641 ttcttaggct cataggggat agtcaccaga tagtcctcag catcaaagcg agacttgaca
    14701 gggttgtaaa agagtacata gtcctcaaaa acgatgtcct cgctactcgt ctccccctgg
    14761 tcgtccacgg tttcaatagc agagctgttt tgtgcttcta atataaaagt gacttctaca
    14821 gtatgatttt tcttatccca atccatggca aagtcatact gtaaatgctt atccaattct
    14881 tcttctagta ccgataaaaa tccaaattta gccatcttat gtccttattt tctatttatt
    14941 ctttcttatt ataacatgaa atagaagaaa aacgttacag actcaggtta atcaaaaggt
    15001 agttcctact tatttttatg ttacaatagt ctcaaagact agccgttttt gactgacttc
    15061 atactatgtt aaaagcgtca gccaagagaa aaactctata tagaaaatag aaaggatttg
    15121 aatacgggac taattttcta gaagaaaaga tgaacttcct aatgtctgac gacacttcgt
    15181 cgttccctat ttctttacga aaattttcgg taaaccgaac cgtccctttg tatcatcaat
    15241 tatgattatc agacctatgc aggactgcga ccgtgaggca gttctgacct tacaaaatac
    15301 tggctggact gctcttacct ctcctgtcta tgaccgcaag tggactgaga gtgatttgga
    15361 gaagaatctg gcaaatggca tgtccttctt tgtcgctgag gtcgatgata agatcgctgg
    15421 cgtcttggac tttggtccct actatccttt cccagctggc aagcatgttg ccactttcgg
    15481 cattctcatc gctgaaccct accaaggtca gggactgggt aaagctctct tgaaagctct
    15541 gctaactgag gctaaagctc aaggttacat caagattgcc atgcatgtca tgggcaataa
    15601 cagcagagcc atttccctct atcaaaaata cgggtttacc gaagaggctc gaatcactaa
    15661 agcctttttc attgaaaatc attatgttga tgctttaatt ttcgcaaaag atttggagaa
    15721 taatcatggc tgacaatctt gctctacgta tgcgtcctcg caatattaat gaagttatcg
    15781 ggcagcaaca tctcgttggt aatggcaaaa ttattgatcg aatggttgca gctaatatgt
    15841 tatcttccat gatcctatat ggcccaccag gtattgggaa gacttctatt gcttctgcta
    15901 ttgctggtac tactaaatat gcctttcgta cttttaatgc cactgttgat agtaaaaaac
    15961 gtcttcaaga aattgcagaa gaagctaaat tttctggtgg cttagtttta ttacttgacg
    16021 agattcatag acttgacaag acaaaacaag attttctttt acctctactt gagaatggca
    16081 atattattat gattggagca acaactgaaa atcctttctt ttcagtaact cctgccatta
    16141 gaagtagggt acaaattttt gagttagaac ctctttctaa cgaggatatt aaaaaagcca
    16201 ttcagctagc aatttccgat aaagaacggg gatttccttt tttagtgaca attgatgatg
    16261 aggcgctaga ttttattgtt accgccacaa atggagacct acgatctgct tataattcct
    16321 tggatctagc agtcatgtct acttctccta atgaagatgg tagccgtcat attagtctag
    16381 agacaatgga aaatagtctg cagcgtagtt atattacaat ggataaaaac ggagatgggc
    16441 attatgatat cctatcagcc ttacaaaaat caattagagg ttctgatgtc aatgccagcc
    16501 tgcactatgc cgctcgttta gtagaagccg gagatttacc tagtttagca agacgtttga
    16561 ccattattgc ttatgaagat atcggcttag ctaaccccga agcacaaata catactgtga
    16621 ctgcattgga agctgcccaa cgcattggtt ttccagaagc tcgtattctt attgctaaca
    16681 ttgttgtgga tttagctcta tcacctaaat caaattcagc ctatttggct atggatgcag
    16741 ctttagctga tttacgtcgt tcaggaaatt tacctattcc acgtcaccta agagatggac
    16801 attacagcgg tagtaaaacc ttgggcaatg caagggatta caagtaccct cacgcctatc
    16861 ctgaaaaatg ggttaaacaa caatacttac cagataaact agtaggacat aattactttg
    16921 aagccaatga aactggtaaa tacgaacgcg cacttggttc aaataaagag cgcattgata
    16981 agctctcaga ctgattgcta gttttaaaaa tcgcaatcga tttcaaaaat cgataatttt
    17041 gcttgaaaat tttataaaaa gtggtatcat aaagacatag aaacattgct gtggcatacg
    17101 aatccacatc taagtgttga ccgactattt ctttgtattt cgggaaacga agtcatctct
    17161 agtgcgcaag ccgttaacac gcggaagcca cttaaagagg tgcgagttac ccacctgctt
    17221 attttgtgcg ggatcaatac aaaaagtgga tcatcggtgc caatacagct tcctagcctc
    17281 cttagctcag ttggtagagc agtagactct taatctatgg gtcacaggtt cgagccctgt
    17341 agggggcact aataatatag ttgaaaacct tgattttaaa ggtttttttc tttgtttcag
    17401 cagatttgtt ctaaatttgt tctagaagat attctttaaa aattagcttt aagatactaa
    17461 acttctgacc gtttcatatt ccttatttat tacttctgtc aagacgtgac cataagtttc
    17521 aagtaactgt gtaatatcct tatgacccat taatcttgca actacccaaa tatcaacacc
    17581 cttagccaat aaataacttc cataggtatg tctcgcacct attgcagtca tttttgaatc
    17641 aatatttaat aaatgtaata catttctcaa acttttatta atggcactat ttgacgacac
    17701 accgtaccta tagtcataaa atatttgatt attaatattt acaattttta agtttttcaa
    17761 cataacttgc tgatcatcct tcaaacgtct taaggttaat gccaaagatt gactaatagg
    17821 aatagtacga attgaggttt ttgttttagg tggactaaaa gttcccttat ccccactaaa
    17881 tcttctatac gtcttgattt ctagatcttc aaaattaaca tcatcccaag ttaaagctaa
    17941 agcttccccc actcgtaaac ctgttttaaa tagcaccagt aataaataat aaacaatact
    18001 atttgaataa tccaaattgt tttctaaata agatatcact ttcttgtaat cagaaatact
    18061 atgtagatat ttatcatctg catccttaac aaatttccta ccagctatga caactccatc
    18121 agtaaaatca gataataaga caccatctct tttagcaaat tggatagctt tttttatatc
    18181 tgaatttaac cttctaacat ggtttttagt gtatttttca gcgtacatat ttaatttcct
    18241 ctgatacgca ctatgcttta ttttatttac tttctgattt ccaaaaagtt tacgaataac
    18301 agaacctctt gtaaaatatt tgttctttgt agtctctgct agattgctag gctttataat
    18361 taaactgtac cattcaaacc acaaatcata caatgttata tcagaagata tagaatttac
    18421 taataacttt tgttgctcta tcctcatagc ttcattcata gcttcacgct ttgtcttaaa
    18481 acctgaacca gaagcaacta actcactttt ttcattgaat attctataat cccataattt
    18541 ctttttacct ctttgtctat atgttgccat tttactcctt tcttagacat caaggaattt
    18601 atcctcaata ttataccatt taactattta ataaaataga accaaaatta tcttcaaaat
    18661 attttctagc ttttgttgct aatattttat atcgactacc tttacctttc ggataaacaa
    18721 taaaaccatt tggattaatt gataaatcta cttctgtttt tatatctgga tttaacaaaa
    18781 tatcattaat caattttgac cttccaattc ccaataactc ttgtaagtta tcaatatccc
    18841 accacttgcc ttgttcatta tcacttaaca aatttaaata ttcttctttt gaaataataa
    18901 caacatcatc tggtacagga acactcacat taaaaacctg ttgcatactc atcctcctta
    18961 taacttgctt aatccgaacg ttatttgttc caggtaatta ggttttccac tttaaaaccg
    19021 aactattttt caattgcagt tttaatttaa aatccatatc ccccgaacat tacgctttaa
    19081 taaaaaaaca aacaaaaaaa gccatgaaat taatcacaac tttatttcta tcaacttctt
    19141 atccttcaag atcatttaat attcttctta aagtactttt tatttcttct gcttcgctat
    19201 aaccgccatc ctcaaaccta gataattccc tttcaaaact tgaaattgat ttacgtaatt
    19261 ttgatatttc atcattttta ggttgatttg ataaatcgac tattttttcc tctaaagaat
    19321 taacttttaa gcctaacatt tgattttctc tgcttacttc accaaacttc aaaattaatt
    19381 cattagcaaa agccattttt tcaataagtt tatctccatt tttcacatga ttataaactt
    19441 cttcccttga ctttttctca ataagcattt catcacggat atatttagta tcttctttaa
    19501 catatgagtt tttttgaact aattcttgtt tattcttttc aatattgttt gataaattgc
    19561 tttgtatcag aacatattta tcatattgat ttgtcgaaat tttggaaaat tcgtctacca
    19621 atttctcttg accttcttct aaaagctcaa gttctcgttg aatagcttta ttatccctag
    19681 agtttgaaat caaaactccc acatttgtaa aaataattcc tacaacacca cctaaagtag
    19741 cccaatccat tactgaataa tcccctatca ttttttctcc tattctatca cattttattc
    19801 cattctgtat tatttaatta aaaaagcc
//
LOCUS       DBJYSH010000026        19545 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.026, whole genome shotgun sequence.
ACCESSION   DBJYSH010000026 DBJYSH010000000
VERSION     DBJYSH010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 19545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19545
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.026"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            183..794
                     /gene="rpsD"
                     /locus_tag="ACUBV3_000752"
     CDS             183..794
                     /gene="rpsD"
                     /locus_tag="ACUBV3_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HHU3847271.1"
                     /translation="MSRYTGPSWKQSRRLGLSLTGTGKELARRNYVPGQHGPNNRSKL
                     SEYGLQLAEKQKLRFSYGLGEKQFRNLFVQATKAKEGTLGFNFMVLLERRLDNVVYRL
                     GLATTRRQARQFVNHGHILVDGKRVDIPSYRVTPGQVISVREKSMKVPAILEAVEATL
                     GRPAFVSFDAEKLEGSLTRLPERDEINPEINEALVVEFYNKML"
     gene            1038..1334
                     /locus_tag="ACUBV3_000753"
     CDS             1038..1334
                     /locus_tag="ACUBV3_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4298 domain-containing protein"
                     /protein_id="HHU3847272.1"
                     /translation="MNLNDRLKIEEMEEKYDSFKPRINALVEAIDDFQKHYEDYVKLR
                     EFYGSEDWFRLSEQTENNLKCGVLSEDQLFDFIGEHNELVGQFLDMSSQMYRHL"
     gene            complement(1420..1959)
                     /locus_tag="ACUBV3_000754"
     CDS             complement(1420..1959)
                     /locus_tag="ACUBV3_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HHU3847273.1"
                     /translation="MVNDTRREKTKRAIEAAMITLLKDQSFDEISTINLTKTAGISRS
                     SFYTHYKDKYEMIDQYQQSLFNKVEYIFDRNQFKKEDALLEIFQFLDRESLFAALLTQ
                     NGTKEIQTYILNKLQLMLSKELPVVNPDATKSDINRLYYSVYLSHAIFGVYQMWITRG
                     KKESPQQITQVLLSLLPQT"
     gene            2098..4416
                     /locus_tag="ACUBV3_000755"
     CDS             2098..4416
                     /locus_tag="ACUBV3_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000883428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HHU3847274.1"
                     /translation="MLDELKAIIKSPKLWITMAGVALIPTLYNVIFLSSMWDPYGNTK
                     NLPVAVVNQDKSAKLNGKTISIGKDMEDNLSKNDSLDFHFTTAKRAEKELEKGHYYMV
                     ITFPKDLSRKATTLMTEKPERLNITYKTTKGRSFVASKMSETAANKLKDEVAESITGT
                     YTESVFKNMGSMKTGINKAADGSQELLNGSNKLQDGSQTLTSNLDVLASSSQTFSGGA
                     NKLNSGINLYTDGVGTLSNGLETLSDGVTAYTTGVHKLSEGSQKLDDKSQALVEGSEK
                     LTDGLQQLSQATQLKPEQERTLQNLSDGLKNLNQIITNLQSTATTDSDTNSKLFNFLS
                     TIESSTKALMNTAAADKQKQMTAVQSTSAFKSLTPEQQSQITSAVTGTPTSAETIAAN
                     ISSNIENMKTVLSEASSSAPSNNGSQNLQTLSGTANNLVLKAISDLDKIQKLPTATKQ
                     LYQGSQTLTKGITDYTNAVGQLRKGAVTLDSKSNQLISGTQKASQGAQTLDSKSDQLR
                     DGAGQLASGSDRIADGSNKLAGGGHQLTDGLTELSGGVSQLSSSLGKAGDQLSMVSVN
                     KDNANAVSSPVTIKHEDYDSVDTNGVGMAPYMISVALMVVALSANVIFAKALSGKEPA
                     NRFSWAKNKLLINGFIATLAATILFFAVQFIGLKPDYPGKTYFIILLTAWTLMALVTA
                     LVGWDNRYGSFLSLLILLFQLGSSAGTYPIELSPKFFQTIQPFLPMTYSVSGLRETIS
                     LTGDVNHQWRMLVIFLVSSMILALLIYRKQED"
     gene            4483..4839
                     /locus_tag="ACUBV3_000756"
     CDS             4483..4839
                     /locus_tag="ACUBV3_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HHU3847275.1"
                     /translation="MSTLTIIIATLTALEHFYIMYLETLATQSNMTGKIFSMSKEELS
                     YLPVIKLFKNQGVYNGLIGLFLLYGLYISQNQEIVAVFLINVLLVAIYGALTVDKKIL
                     LKQGGLPILALLTFLF"
     gene            4973..5716
                     /locus_tag="ACUBV3_000757"
     CDS             4973..5716
                     /locus_tag="ACUBV3_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HHU3847276.1"
                     /translation="MTENWLHTKDGSDIYYRVVGQGQPIVFLHGNSLSSRYFDKQIAY
                     FSKYYQVIVMDSRGHGKSHAKLNTISFRQIAVDLKDILVHLEIDKVILVGHSDGANLA
                     LVFQTMFPGMVRGLLLNSGNLTIHGQRWWDILLVRIAYKFLHYLGKLFPYMRQKAQVI
                     SLMLEDLKISPADLQHVSTPVMVLVGNKDIIKLNHSKKLASYFPRGEFYSLVGFGHHI
                     IKQDSHVFNIIAKKFINDTLKGEIVEKAN"
     gene            5700..8240
                     /gene="mprF"
                     /locus_tag="ACUBV3_000758"
     CDS             5700..8240
                     /gene="mprF"
                     /locus_tag="ACUBV3_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HHU3847277.1"
                     /translation="MKKLIEKVKSLTSVIKIVFFISVLVLIIVEMIHLKRTISVEQLK
                     SVFGQLSPMNLFLIILVGVIAVLPTTGYDFVLNGLLRTDKSKRYILQTSWCINTFNNL
                     SGFGGLIDIGLRMAFYGKKGQEKSDLREVTRFLPYLISGLSFISVIALIMSHIFHAKA
                     SVDYYYLVLIGASMYFPVIYWISGHKGSHYFGDMPSSTRIKLGVVSFFEWGCAAAAFI
                     IIGYLMGIHLPVYKILPLFCIGCAVGIVSLIPGGLGSFELVLFTGFAAEGLPKETVVA
                     WLLLYRLAYYIIPFFAGIYFFIHYLGSQINQRYENVPKELVSTVLQTMVSHLMRILGA
                     FLIFSTAFFENITYIMWLQKLGLDPLQEQMLWQFPGLLLGVCFILLARTIDQKVKNAF
                     PIAIIWITLTLFYLNLGHISWRLSFWFILLLLGLLVIKPTLYKKQFIYSWEERIKDGI
                     IIVSLMGVLFYIAGLLFPIRAHITGGSIERLHYIIAWEPIALATLILTLVYLCLVKIL
                     QGKSCQIGDVFNVDRYKKLLQAYGGSSDSGLAFLNDKRLYWYQKNGEDCVAFQFVIVN
                     NKCLIMGEPAGDDTYIREAIESFIDDADKLDYDLVFYSIGQKLTLLLHEYGFDFMKVG
                     EDALVNLETFTLKGNKYKPFRNALNRVEKDGFYFEVVQSPHSQELLNSLEEISNTWLE
                     GRPEKGFSLGYFNKDYFQQAPIALVKNAEHEVVAFANIMPNYEKSIISIDLMRHDKQK
                     IPNGVMDFLFLSLFSYYQEKGYHYFDLGMAPLSGVGRVETSFAKERMAYLVYHFGSHF
                     YSFNGLHKYKKKFTPLWSERYISCSRSSWLICAICALLMEDSKIKIVK"
     gene            complement(8319..9833)
                     /locus_tag="ACUBV3_000759"
     CDS             complement(8319..9833)
                     /locus_tag="ACUBV3_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019319700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HHU3847278.1"
                     /translation="MTTLITTFQERFGDWTQSLIEHLQLSLLTLILATLIAIPLGIII
                     SHYKKISHVVLQITGIFQTIPSLALLGLFIPFMGIGTVPAVVALIIYALFPILQNTVT
                     VLMQIDANLIEAATAFGMTRWERLKKFELALSMPVIISGIRTASVMIIGTATLASLIG
                     AGGLGSFILLGIDRNNPSLILIGAISSAVLAIIFSGLIGLLEKARLRTIAVSGILLLA
                     GLGLSYAPKWMPGTNTATITVAGKLGTEPDILINMYKELIEDQTDIKVKLKPNFGKTT
                     FLYQALKSGDIDLYPEFTGTITSSLLKNPPKVSNNPKQVYNLAKNGILKQDKLSLLSP
                     MAYQNTYAVAVKKDYAEANQLKNISDLKKLDKLKAGFTLEFKDREDGSIGLQKHYGLN
                     LDISTLEPALRYQAINSKDVNIIDAYSTDSELIQYQLQILKDDKHLFPPYQGAPLLRQ
                     DTIKKYPQVKKALNKLAGHITEKEMQEMNYQVAVKHKSAATVAKQYLKAHHIIK"
     gene            complement(9836..10558)
                     /locus_tag="ACUBV3_000760"
     CDS             complement(9836..10558)
                     /locus_tag="ACUBV3_000760"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HHU3847279.1"
                     /translation="MISFENVSKSYGDHTIIDNISCHIQRGEFFVLVGASGSGKTTIL
                     KMINRLIEPSQGAITLDGENITSLDLRQLRLETGYVLQQIALFPNLTVGENIELIPEM
                     KGWSKGDQKKAASDLLDKVGLPAKDYFNRYPHELSGGEQQRIGILRAIVAKPKVLLMD
                     EPFSALDPISRRQLQDITKQLQSELGITLVFVTHDMKEAMRLADRICVIKEGKIVQLD
                     RPEIIQNNPSDQFVRTLFEEEN"
     gene            complement(10780..11613)
                     /locus_tag="ACUBV3_000761"
     CDS             complement(10780..11613)
                     /locus_tag="ACUBV3_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000675243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HHU3847280.1"
                     /translation="MKFYIIDDDPTITMILQDIIEEDFNNTVVRVNNVSSKAYNELLI
                     ADVDIVLIDLLMPILDGVTLVQKIYKQRSDLKFIMISQVKDNDLRQEAYKAGIEFFIN
                     KPINIIEVKSVVKRVTDTIEMQKKLNTIQNLLENTPSYQKPITTSNLTKIRSILSYLG
                     ITSETAYTDILNICELLLKQELNFAQFDFQKELSIDEHQQKIILQRIRRAVKKAMINM
                     AHLYIDDFENELTLQYANALFGFQNIHNEAQLIQGKSMYGGKISLKHFFDELILQSKT
                     F"
     gene            complement(11610..12905)
                     /locus_tag="ACUBV3_000762"
     CDS             complement(11610..12905)
                     /locus_tag="ACUBV3_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HHU3847281.1"
                     /translation="MLDILSEKHLERRQRIIISAIAIALAAQINISILADGFIMTLSL
                     FILPVFLYFNDDINPILLCLGITFASPIFRGIILSIAGEAEIHQIIEFVLTDMAFYIC
                     YGITFYTIYWHRSYRNKGTFFFSIIICDYFANLVEISFLIKFNNYTITIFATLFAIAL
                     LRAFISCAVAYTYSYLSLLLQKDDHERRYYYFMWSTSAVKSEVYFMQKNIIEIENIMK
                     NAYLLDKELSKYHLPKEYQHLSLDISRDVHEVKKDYQNIIKGLGTYFSVKNESTMALK
                     DIFQIVLSYTRSIIQFRHQDIIILENNKCNLIISNYYYLLTIISNIVLNAVEAIDKQK
                     KGTISVHTEELEDFIKIEISDNGPGIPDKMKHMIFKPGFSTKFDANGDIYRGIGLSHV
                     RILMEEQYQGTITVCPNQPNGTTFTLLFRKNRFKLGDNT"
     gene            13008..14006
                     /gene="argF"
                     /locus_tag="ACUBV3_000763"
     CDS             13008..14006
                     /gene="argF"
                     /locus_tag="ACUBV3_000763"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003045350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - L-arginine biosynthetic process
                     via ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HHU3847282.1"
                     /translation="MKNLRNRSFLTLLDFSTAEVEFLLKLSEDLKRAKYAGIEQQKLV
                     GKNIALIFEKDSTRTRCAFEVAAHDQGAHVTYLGPTGSQMGKKETSKDTARVLGGMYD
                     GIEYRGFSQETVETLAEFSGVPVWNGLTDADHPTQVLADFLTAKECLHKPYKDIRFTY
                     VGDGRNNVANALMIGASIVGMTYHLVCPKELEPDPELLSKCQEIAKTTGASIEITADI
                     AEGVRDSDVLYTDVWVSMGEPDEVWKERIALLEPYRITQEMLNMTENPNVIFEHCLPS
                     FHNIDTKVGYDIYEKYGLKEMEVSDEVFEGPHSVVFQEAENRMHTIKAVMVATLGD"
     gene            14018..14944
                     /gene="arcC"
                     /locus_tag="ACUBV3_000764"
     CDS             14018..14944
                     /gene="arcC"
                     /locus_tag="ACUBV3_000764"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HHU3847283.1"
                     /translation="MAKIVVALGGNALGNSPEEQLRLVKHTAKSLVALIKKGHEIVVS
                     HGNGPQVGAINLGMNFAAESGQGTNFPFPECGAMSQGYIGYHLQQSLLNELRQEGINK
                     EVATIITQIEVDESDQAFSAPTKPIGTFYDKETSEKIAIEKGYTFVEDAGRGYRRVVA
                     SPEPKKIIEINSIKTLIENDTLVIAGGGGGIPVINKGGYEGIAAVIDKDKSSALLAGE
                     LAADQLIILTAVDYVYTQFGKENQKALTEVNENQMIDYVNQGEFAKGSMLPKVIACMS
                     FLDHNPKGTALITSLNGLEDALDGKLGTRITK"
     gene            15038..16591
                     /locus_tag="ACUBV3_000765"
     CDS             15038..16591
                     /locus_tag="ACUBV3_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HHU3847284.1"
                     /translation="MPEKMTNSENKRSKGFRMPGAFTILFILTIFSVLATWWIPAGSY
                     SKLQFDTASSKLVVTDPNGKTVHVPATQTQLDKMNVKIKIKEFTSGAISKPVSVPNTY
                     KRLKQNPAGIGSVTTSMVNGTIEAVDIMVFIMVLGGMIGVVRKSGAFESGLLALTKKT
                     KGREFLLIFLVSLLMVLGGTLCGIEEEAVAFYPILVPIFLAMGYDSIICVGAIFLASS
                     VGTSFSTINPFSSVIASNAAGISFTEGLSWRTAGCIAGAIFVVVYLHWYAKKIKANPE
                     FSYSYEDRVEFNAKWGMTTNHTPSLFTIRQKIILSLFVISFPLMVWGVMSQGWWFPTM
                     ASSFLAITIIIMFLTATGANGIGERDVVDEFVNGASSLVGVSLIIGLARGINIILSQG
                     YISDTMLYTASKLASHVSGSVFIIVMMFIYFVLGFVVPSSSGLAVLSMPILAPLADTV
                     GIPRSVVVMAYQFGQYAMLFLAPTGLVMATLQMLDMKYSHWLKFVWPVVLFLLIFGGG
                     LLVLQVLIL"
     gene            complement(16701..18065)
                     /locus_tag="ACUBV3_000766"
     CDS             complement(16701..18065)
                     /locus_tag="ACUBV3_000766"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000898851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HHU3847285.1"
                     /translation="MLFQSKINYPKKSSIITQITLWYSSFIFILVIGVLIGSFFISKS
                     IAENKSKKNLEAKAVQMSQALAKGHRYEAFEDGIFYSVYDQNGKVIYSGFPKGFKRDL
                     DHQHKHKKKLSLFSMENRTFQYVDIPISGKNQWLRAIRTVDRLDKQLTELLFSLGIVL
                     PLMLIIITVGGYLILKRTFRPIQEITETAQFITQNEDYTKRIITKNNENELTELAAVI
                     NTMLASIESSFVREKQFNNDVSHELRTPVTVILSESEYGKNYAENLSEAKESFEVIHR
                     QSLSMKKLVEQLLELTKAENPLSIQLEPLNFSIMMKQLVSDSSRLLDNTPIHLDSQIE
                     DDLWIIGQQTLLKRLFDNLFSNAIKFTNNHISISLRQSDNQIVFSIKDNGLGISVDDQ
                     SKIWNRFYQVDSARTKDSQSGIGLGLSLVKQIATIHRAKIWVDSKPDDGSQFTLTFPK
                     LKKD"
     gene            complement(18049..18720)
                     /locus_tag="ACUBV3_000767"
     CDS             complement(18049..18720)
                     /locus_tag="ACUBV3_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HHU3847286.1"
                     /translation="MKILVVEDEFDLNRSIVKLLKKQHYSVDSASNGEEALQFVSVAE
                     YDVIILDVMMPKMDGFTFLKLLRNKGSQVSILMLTARDAVEDRIAGLDFGADDYLVKP
                     FEFGELMARIRAMLRRANRQVSSDDIQIQDITINLSTKQVWRNDNLIDLTAKEYEVLE
                     YLARHRDQVLSRHQIREHVWDYDYYGESNIIDVLIKNLRRKLDNNRDGSLIKTKRGLG
                     YVIPK"
     gene            complement(18805..19476)
                     /locus_tag="ACUBV3_000768"
     CDS             complement(18805..19476)
                     /locus_tag="ACUBV3_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HHU3847287.1"
                     /translation="MTNYKNNFKKFMSNKLYVGLASITLLGIGGAIGVSGAALAHNHH
                     SEHMSFEHKHEGHDKHHDHREKYSLKTLKNISTKIDYKTALETAKKEARSGFISQIEL
                     NTKGDTPFYEIKVIDNKIAKKYKIDANSGKILSTTSDNQLNSKDAQLKQPSKDIQTAL
                     QAIQAKYKNAKIKEISLANNKSNKLVYKVKLLDGNTPKIAQFDSDKASFIKANKLNTT
                     HKNHD"
BASE COUNT         6170 a         3031 c         3643 g         6701 t
ORIGIN      
        1 tgattcaata ttattttcat gatataatat agcttgtgtg aaataacagc agaaaaatat
       61 gaagctcgtc aacaggtggt taactaatct agtacccttt gctgtttagg cgaaaatccc
      121 ctgcacgaat caggattttt taaatcgtta tttcctcaat tattttagag gaggacattt
      181 agatgtcacg ttatactggt ccatcatgga aacaatcacg tcgccttggt ttatcactta
      241 caggcacagg taaagaattg gcacgtcgta actacgtacc tggtcaacac ggtcctaata
      301 accgtagcaa gctttcagaa tatggcttgc agttagctga gaaacaaaaa cttcgttttt
      361 catacggact tggtgaaaaa caattccgta acttgttcgt acaagctact aaagcaaaag
      421 aaggaactct tggtttcaac tttatggttc ttttagagcg tcgtcttgat aacgttgttt
      481 atcgtttagg acttgcaaca actcgtcgcc aagctcgtca atttgttaac catggtcata
      541 tccttgttga tggtaaacgt gttgatatcc catcataccg cgttactcca ggacaagtta
      601 tttcagttcg tgaaaaatca atgaaagtac ctgcaatcct tgaagctgtt gaagctactt
      661 taggacgtcc agctttcgta tcatttgatg ctgaaaaact tgaaggttca ttgactcgcc
      721 ttccagaacg cgatgaaatt aacccagaaa tcaatgaagc acttgtcgtt gaattctaca
      781 acaaaatgct ttaattatta cattacatac tttaaaagcc tattttatag gctttttttg
      841 ctatatactt tttagtgagt cattatattt aatgaaccaa aaagatttta aactaacatt
      901 agaataaaaa tcaacactgt cagtttgtaa tgagtaattt tttactttgt aaaaaggata
      961 tagtagagtt aacgttacaa atacttgaga atgataactt atctcgtcga atcttatata
     1021 actaaggaga ttttattatg aatctaaatg atagattaaa aatagaagaa atggaagaga
     1081 agtatgatag cttcaaacct agaatcaatg cattagtaga agctatagat gattttcaaa
     1141 agcactatga agattacgtg aaattaagag aattttatgg tagcgaagac tggtttagac
     1201 tatcagaaca aacagaaaat aatctcaaat gcggtgtact gagtgaggat cagctctttg
     1261 attttatagg agaacataac gagcttgtag gccaattttt agatatgtct tctcaaatgt
     1321 atcgtcattt ataatgtcag tccttattag tgttgttatc ctataatgag gataaaataa
     1381 acctaactaa aaatgaccag cctataagga aggctggttt taagtttgag gtaataaact
     1441 gagtagcact tgtgtaattt gttgagggct ttcttttttt cctcgtgtta tccacatttg
     1501 atagactcca aaaatagcat gactgagata gacactataa tacaaacggt tgatatcaga
     1561 ctttgttgca tctggattga ccacaggtaa ttctttagaa agcattaatt gtaatttatt
     1621 aaggatgtag gtctgaattt cttttgttcc attttgggtt aagagagcag caaataatga
     1681 ttctctatct aagaattgaa aaatttcgag caaggcatct tcttttttaa attgattgcg
     1741 atcaaaaata tattcgactt tattgaagag tgactgttgg tattgatcaa tcatttcata
     1801 tttatcttta taatgagtat aaaaactaga acgactgatt ccagctgttt tagttagatt
     1861 aatcgttgaa atttcatcga agctctgatc ttttaataac gtaatcatag ctgcttcaat
     1921 agctcttttt gttttttctc tcctcgtatc gtttaccata ttaattaccc cttaatattt
     1981 ttgtacattt ttaaacacag tgtccaaaaa cgagattttt atcttgaaat tatctatcat
     2041 cactgtataa tatattttat caaaaaaatg aacaatatgt ccaaaatagg agaaaaaatg
     2101 ttagacgaat taaaagcaat cataaaaagc ccaaaattat ggattacgat ggctggggtt
     2161 gcactgattc caacgctata taatgttatc tttctaagct caatgtggga tccttatggg
     2221 aatactaaaa atttacctgt ggcagtcgtt aaccaagata aatcggctaa attaaatgga
     2281 aaaactattt caattggcaa agatatggag gacaatttgt ctaaaaatga tagcttagat
     2341 ttccatttta caacagctaa aagagcagaa aaagaattag aaaagggtca ctattatatg
     2401 gttattacct ttcctaaaga tctttctaga aaagcgacaa ctctcatgac agaaaaacca
     2461 gaaaggttaa atattactta taaaactact aaggggcgta gttttgtagc ttcaaaaatg
     2521 agtgagacag cagctaataa actaaaagat gaggtagcag aatctattac agggacttat
     2581 actgaatcag tttttaaaaa tatgggttct atgaaaacag gtatcaataa agctgctgat
     2641 gggagtcaag aattattaaa tggttctaat aagctacagg atgggagcca aaccttgaca
     2701 agtaatttag atgttttagc aagctctagt caaacttttt ctggaggagc taataaacta
     2761 aatagtggaa taaaccttta tacagatggt gtggggaccc tatctaacgg gttagaaaca
     2821 ttatcagatg gtgttactgc ttacacaaca ggtgtgcata agttgagtga aggtagtcaa
     2881 aaattagatg ataagtctca agcattggtg gaaggatcag aaaagttgac ggatggttta
     2941 caacaattgt cacaagcaac tcaactaaaa ccagagcaag aacgtacttt gcagaacctt
     3001 tcagatggat taaagaatct aaatcagatt attacaaatt tacaatcgac agcaactact
     3061 gatagcgata ctaatagtaa gttatttaat tttttatcaa ctattgaatc aagtacaaag
     3121 gctttgatga atacggctgc tgctgataaa caaaagcaaa tgactgcggt acaatcaacg
     3181 tcagctttta aaagtttaac ccctgaacaa caatctcaaa tcacctcagc ggttactgga
     3241 acacctactt cagctgagac aattgcagca aatatttctt caaatataga aaatatgaaa
     3301 actgtgctta gtgaagcatc tagttcagcc ccttctaata atggatcaca aaatcttcaa
     3361 actttgtcag gaacagctaa caatttagta ttaaaagcta tctcagattt agataaaatt
     3421 caaaaattgc caacggctac taagcagtta tatcaaggta gtcaaacctt gacaaaagga
     3481 atcacggatt atacgaatgc tgtagggcaa ttacgcaaag gtgcagtaac tttagatagt
     3541 aaatccaatc aattgatttc aggaacacaa aaagcaagtc agggagctca aactttagat
     3601 agtaaatcgg atcaattgag agacggagcg gggcaattag ctagtggttc tgatagaatt
     3661 gcagatggtt ctaataagct tgctggggga ggacatcagt tgacagatgg gttgacagag
     3721 ttgtcaggtg gtgtttctca attatcaagt tctttgggga aagctgggga ccagttgtcc
     3781 atggtttctg taaataaaga taatgccaat gcagtatcta gcccagtcac tatcaagcat
     3841 gaagattatg atagcgttga tacgaatggt gttggaatgg caccatacat gatttcagtt
     3901 gcgttgatgg tggtagcttt atcagccaat gttatttttg cgaaagcttt atctggcaaa
     3961 gagccagcta atcgttttag ttgggctaaa aataaattat taatcaatgg attcattgca
     4021 actctagcag caactatctt attttttgca gttcaattca taggtcttaa accagattac
     4081 cctggaaaaa cctactttat tatcctattg acagcatgga ctttgatggc attagtaact
     4141 gctttagtgg gatgggataa taggtatggt tccttcttgt cgttattaat attattattc
     4201 cagcttggtt caagcgcagg aacttaccca atagaattga gtcctaagtt ctttcaaaca
     4261 attcaaccat ttttaccgat gacttactct gtttcaggat taagagagac catctcgttg
     4321 acgggagacg ttaaccatca atggagaatg ctagtaatct ttttagtatc atcgatgata
     4381 cttgctcttc ttatttatcg taaacaagaa gattaataga aagtatctag tgatagacta
     4441 acagtatgat atggtatgtc aaagtattta ggaggagaag atatgtctac tttaacaata
     4501 attattgcaa cattaactgc tttggaacat ttttatatta tgtatttgga gacgttagcc
     4561 acccagtcaa atatgactgg gaagattttt agtatgtcta aagaagagtt gtcatattta
     4621 cccgttatta aactttttaa gaatcaaggt gtatacaacg gcttgattgg cctattcctc
     4681 ctttatgggt tatatatttc acagaatcaa gaaattgtag ctgttttttt aatcaatgta
     4741 ttgctagttg ctatttatgg tgctttgaca gttgataaaa aaatcttatt aaaacagggt
     4801 ggtttaccta tattagctct tttaacattc ttattttaat actacttagc cgttcgattt
     4861 agttgaacgg cttttagtaa tcattttttt ctcataatac aggtagttta agtaatttgt
     4921 ctttaaaaat agtataatat aactacgaat tcaaagagag gtgactttga ttatgactga
     4981 gaactggtta catactaaag atggttcaga tatttattat cgtgtcgttg gtcaaggtca
     5041 accgattgtt tttttacatg gcaatagctt aagtagtcgc tattttgata agcaaatagc
     5101 atatttttct aagtattacc aagttattgt tatggatagt agagggcatg gcaaaagtca
     5161 tgcaaagcta aataccatta gtttcaggca aatagcagtt gacttaaagg atatcttagt
     5221 tcatttagag attgataaag ttatattggt aggccatagc gatggtgcca atttagcttt
     5281 agtttttcaa acgatgtttc caggtatggt tagagggctt ttgcttaatt cagggaacct
     5341 gactattcat ggtcagcgat ggtgggatat tcttttagta aggattgcct ataaattcct
     5401 tcactattta gggaaactct ttccgtatat gaggcaaaaa gctcaagtta tttcgcttat
     5461 gttggaggat ttgaagatta gtccagctga tttacagcat gtgtcaactc ctgtaatggt
     5521 tttggttgga aataaggaca taattaagtt aaatcattct aagaaacttg cttcttattt
     5581 tccaaggggg gagttttatt ctttagttgg ctttgggcat cacattatta agcaagattc
     5641 ccatgttttt aatattattg caaaaaagtt tatcaacgat acgttgaaag gagaaattgt
     5701 tgaaaaagct aattgaaaaa gtcaaatcac tgacttctgt gattaaaatt gtatttttta
     5761 tatctgtttt agtgcttatt attgttgaaa tgattcattt gaaacgaact atttctgttg
     5821 agcaactaaa gagtgttttt gggcaattat ctccaatgaa tcttttctta attatccttg
     5881 tgggggttat cgctgtctta ccgacaaccg gatatgactt tgtactgaat ggacttttac
     5941 gtacagataa aagcaaaagg tatattttac agactagttg gtgtatcaac acttttaata
     6001 acttgtcagg attcggtggc ttaatcgata ttgggttgcg catggctttt tatggtaaaa
     6061 aaggtcaaga gaagagtgac ctaagagaag tgactcgttt tttaccctat cttatttctg
     6121 gtctgtcatt tattagtgtg attgccttaa tcatgagcca tatttttcat gccaaagcta
     6181 gtgttgatta ctattatttg gtattaattg gtgctagtat gtattttcct gttatttatt
     6241 ggatttctgg tcataaagga agccattatt tcggagatat gccatctagt actcgtataa
     6301 aattaggtgt tgtttctttt tttgaatggg gatgtgcggc cgcagcattt ataattatcg
     6361 gttatttaat gggcattcat ctaccagttt ataaaatttt accactattt tgtattggtt
     6421 gtgccgtcgg gattgtatcc cttattcccg gtggattagg aagttttgaa ttagttctat
     6481 ttacagggtt tgctgccgag ggactaccta aagaaactgt ggttgcatgg ttattacttt
     6541 atcgtttagc ctactatatt attccattct ttgcaggtat ctatttcttt atccattatt
     6601 taggtagtca aataaatcaa cgttatgaaa atgtcccgaa agagttagta tcaactgttc
     6661 tacaaaccat ggtgagccat ttgatgcgta ttttaggtgc attcttaata ttttcaacag
     6721 cattttttga aaatattact tatattatgt ggttgcagaa gctaggcttg gacccattac
     6781 aagaacaaat gttatggcag tttccaggtt tattgctggg ggtttgtttt attctcttag
     6841 ctagaactat tgatcaaaaa gtgaaaaatg cttttccaat tgctattatc tggattactt
     6901 tgacattgtt ttatcttaat ttaggtcata ttagttggcg actatctttc tggtttattt
     6961 tactattgtt aggcttatta gtcattaagc caactctcta taaaaaacaa tttatttata
     7021 gctgggaaga gcgtattaag gatggaatca ttatcgttag tttaatggga gttctatttt
     7081 atattgcagg actactattc cctatcaggg ctcatattac aggtggtagt attgaacgcc
     7141 tgcattatat catagcatgg gagccgatag cattggctac gttgattctt actctcgttt
     7201 atttatgttt ggttaagatt ttacaaggaa aatcttgtca gattggtgat gtgttcaatg
     7261 tggatcgtta taaaaaacta cttcaagctt acggtggttc ttcggatagc ggtttagcct
     7321 ttttaaatga taaaaggctc tactggtacc aaaaaaatgg agaagattgc gttgcgttcc
     7381 aatttgtaat tgtcaataat aaatgtctta ttatggggga accagccggt gatgacactt
     7441 atattcgtga agctattgaa tcgtttattg atgatgctga taagctagac tatgaccttg
     7501 ttttttacag tattggacag aagttgacac tacttttaca tgagtatggt tttgacttta
     7561 tgaaagttgg tgaggatgct ttagttaatt tagaaacgtt tactcttaaa gggaataagt
     7621 acaaaccttt cagaaatgcc ctaaatagag ttgaaaagga tggtttctat ttcgaagttg
     7681 tacaatcgcc acatagtcaa gagctactaa atagtttgga agagatttct aatacttggt
     7741 tagaaggacg tcctgaaaaa ggtttctcac taggatattt taataaagat tatttccaac
     7801 aagccccaat agctttggta aaaaatgctg aacacgaagt tgttgctttt gctaatatta
     7861 tgccaaacta tgaaaagagt attatctcta ttgatttaat gcgtcacgat aaacagaaaa
     7921 ttccgaatgg cgttatggat ttcctctttt tatcattatt ctcttattat caagagaagg
     7981 gataccacta ttttgatttg gggatggcac ctttatcagg agttggtcgc gttgaaacaa
     8041 gttttgctaa agagagaatg gcgtatcttg tctatcattt cggtagtcat ttctactcat
     8101 ttaatggttt acacaagtat aagaagaagt ttacaccatt gtggtcggaa cgttatattt
     8161 cttgttctcg ttcgtcctgg ttaatttgtg ctatttgtgc cctattaatg gaagatagta
     8221 aaattaagat tgttaaataa gctttatttg gcaattaaaa agagcatgtc atgcgacatg
     8281 ctctttttaa atcatttaat accattgatt gcttgaatct actttataat atgatgtgct
     8341 tttaaatatt gtttagctac tgtagctgct gatttatgct ttacagctac ttggtagttc
     8401 atttcttgca tttctttttc agtgatatga ccagcaagtt tattgagagc tttttttact
     8461 tgagggtatt ttttaatcgt atcttgcctt agtagcggtg ctccttgata tggcggaaat
     8521 agatgcttat cgtctttgag aatttgaagt tgatactgga ttaattcact atcggtagag
     8581 taagcgtcaa taatattgac atctttacta ttgatagctt ggtatcgtaa agcaggttct
     8641 aaggttgaga tatcaaggtt taaaccataa tgtttctgta aaccaatact cccatcctct
     8701 ctatccttaa attctaaagt gaaaccagct tttagtttat ctaatttttt taggtcgctg
     8761 atatttttga gttgattagc ttcagcgtaa tcttttttaa cagctacagc gtaggtgttt
     8821 tgataagcca taggtgacaa aagtgacaac ttatcttgtt ttaaaatacc gtttttggct
     8881 aagttgtaaa cctgtttagg gttattactt actttaggag gatttttaag taaacttgat
     8941 gtaatagtac ctgtaaactc tgggtaaaga tcaatatcac cagattttag ggcttgatat
     9001 aagaaagttg tttttccgaa attaggttta agcttaactt tgatatctgt ctgatcctca
     9061 ataagttctt tgtacatatt aattaaaata tctggttcag ttcctaactt ccctgcaaca
     9121 gttatcgttg cagtatttgt tccaggcatc cattttggag cataggatag tcctaagcct
     9181 gctagtaaaa gtataccaga aacggctatg gttcgtaatc tagccttttc tagtaatcca
     9241 attaaaccac taaatataat agctaagaca gcagaagaga ttgcccctat gagaattaga
     9301 gaaggattgt tgcgatcaat ccctaacaaa ataaatgatc caaggccacc agctccgata
     9361 agactagcta atgtagctgt accaataatc ataacagagg cagttctaat gcctgagatg
     9421 ataacaggca tacttaaagc taactcaaac tttttaagcc tttcccaacg tgtcattcca
     9481 aaagcggttg ctgcctcaat gagattagca tcaatttgca tcagtactgt tacagtattt
     9541 tgtaaaattg gaaatagagc gtaaataatt agtgcaacga cagctggtac agttccaatt
     9601 cccataaaag gtataaataa accaagcaag gctaaagaag gaattgtttg aaatatccca
     9661 gttatctgaa ggacaacatg actgattttt ttataatgac taatgatgat accgagtgga
     9721 atagcaatta aagttgctaa aataagtgtt agtagtgata gctggaggtg ttcaataaga
     9781 ctttgagtcc aatctccaaa acgctcttga aaggtagtga ttagtgttgt catactcagt
     9841 tctcctcttc aaatagggtt ctaacaaatt ggtcagaggg attattttga attatttctg
     9901 gtctatccaa ttgcactatc ttaccttcct taataacaca aattctatct gcaagacgca
     9961 tagcttcctt catgtcatgg gtgacaaaca ctaatgtaat acccaattca gactgtaatt
    10021 gtttggtgat atcttgtaat tgacgtcttg agatagggtc taaagcagaa aatggctcat
    10081 ccataagtag taccttaggt ttagcaacga ttgctcgtag gatacctatg cgttgttgtt
    10141 ccccaccaga gagctcatga ggatagcgat taaagtagtc cttagcaggt aaacccacct
    10201 tatcaagaag gtcgctagcg gctttttttt ggtcaccttt tgaccagccc ttcatttcag
    10261 ggataagttc tatattttct ccaactgtta aattagggaa aagtgcaatt tgttgcaaga
    10321 cataaccagt ttctaagcgt aattgtcgca aatcaagtga tgtaatgttt tccccatcta
    10381 aagtaatagc gccttggctt ggttcgatga gacgattaat catcttgagg atggtagttt
    10441 taccactgcc actggccccc acaaggacaa agaattctcc tctttgaata tggcaactaa
    10501 tattatcaat gatggtatga tcaccatatg atttactaac attttcaaat gaaatcatgt
    10561 aatttacttt ctagtgcgta acgagtgtag gtcgtatgat attagggtta tattagcata
    10621 aagtagacat tcaaacaact atctgacatt taaattgtca gaaacgataa actagaagca
    10681 aaaacaaaca ccttgcggta gacaaggtgt ttgttggaga tttatgaaaa gaaaagtttt
    10741 aggatggtta tagtatataa ggccatgctt aaacaagtct taaaacgttt tactctgtaa
    10801 aataagttca tcgaaaaaat gtttaagtga aattttacca ccatacatgc tttttccttg
    10861 aataagttgt gcctcattat ggatattctg aaatccaaac aatgcattag catattgcag
    10921 tgttaattca ttttcaaaat catcaatgta cagatgagcc atatttatca tagctttttt
    10981 aacggcacga cgaatacgtt gtaaaatgat tttttgttgg tgttcatcga tagaaagttc
    11041 tttttgaaaa tcaaattggg caaaatttaa ttcttgtttt aataaaagtt cacagatgtt
    11101 aagaatatct gtgtaggctg tctctgaagt aatacctaaa taactgagta ttgaacggat
    11161 tttagtgagg ttactagtcg ttataggctt ttgatatgat ggcgtatttt ctaggagatt
    11221 ttggattgta tttaattttt tttgcatttc gatagtatca gtgacacgtt tcacgacgct
    11281 ttttacttca ataatattaa tgggtttatt aataaaaaat tcgattcctg ctttatatgc
    11341 ttcttgtcgt aaatcattat ctttgacttg ggaaatcata ataaatttta aatcggaacg
    11401 ctgtttatag attttttgga ctaatgttac accgtctaat attggcatta ataaatcaat
    11461 caatacgata tcaacatctg caatcagcaa ttcgttgtat gctttactgg agacattatt
    11521 cactctgact actgtattat tgaaatcttc ttcaataata tcttgtaaaa tcatagtgat
    11581 agtggggtca tcatcaataa tatagaattt cacgtattat ctcctaactt aaatcgattt
    11641 ttacggaata acagtgtaaa tgttgttcca tttggctgat taggacaaac agttatcgtt
    11701 ccttgatatt gctcttccat taaaatacgc acgtgggata gcccgattcc tcgatagata
    11761 tcaccattag catcaaattt agtcgaaaat ccaggtttga aaatcatgtg tttcatttta
    11821 tcaggtattc ctggtccgtt atcactaatc tcaattttaa taaaatcttc taattcttct
    11881 gtgtgaacac tgattgtacc ctttttttgt ttatcaatag cttctactgc atttaaaacg
    11941 atattggaaa taatggttag taaatagtaa tagtttgata ttatcaaatt acatttatta
    12001 ttttcgagaa taatgatatc ttgatgccga aattgaataa tactcctagt ataagataaa
    12061 acaatttgaa agatatcttt caatgccata gttgattcat tttttacact aaaataggtt
    12121 cctaagcctt taataatatt ttggtaatct ttcttaactt cgtgaacgtc ccgcgaaata
    12181 tctagagaga ggtgttggta ttctttaggt agatgatatt tggataactc tttatctaat
    12241 aaataagcat ttttcatgat gttttcaatt tcaatgatat ttttttgcat aaaataaacc
    12301 tcgcttttaa cggctgaagt tgaccacata aaataatagt atcgacgttc gtgatcgtct
    12361 ttttgtaaga gaagtgatag ataactataa gtataagcca cagcgcaact aataaaagct
    12421 cgcaaaagtg ctatggcaaa taaagtagcg aatatagtta tagtataatt gttaaacttt
    12481 atgagaaaac taatttctac aaggttggca aaataatcac aaataatgat agaaaagaaa
    12541 aaagtacctt tattacggta ggaacgatgc caataaatag tataaaaggt gatgccataa
    12601 caaatataaa acgccatatc agttaaaacg aattcaatta tttgatgaat ctcagcctcg
    12661 ccagcgatac ttaatatgat tcctctgaag ataggagaag caaaagtaat tcctagacag
    12721 agaagaattg gattaatgtc gtcattaaaa tataaaaaga caggcaaaat gaacaaagat
    12781 aaggtcataa taaacccatc agcaagtatt gagatattga tttgagcagc taaggcgata
    12841 gctattgcag agataataat acgctgcctt ctttctagat gtttttctga taatatatct
    12901 agcatataaa tgtccttaaa gtttttttga agatttttga tgaattttga ttatttttga
    12961 aagcgatatc tacaataaga gtataaaaaa aaaggaggca ataagcaatg aaaaatttac
    13021 gtaaccgtag ttttcttact ttattggatt ttagtacagc agaagttgaa tttcttttaa
    13081 aactttcaga agatttaaaa cgtgctaaat acgcaggaat tgaacaacaa aaacttgttg
    13141 gtaaaaatat cgctttgatt tttgaaaaag attctacacg gacacgttgc gcttttgaag
    13201 ttgctgctca cgatcaagga gctcatgtta cttatttagg tccaacaggc agtcagatgg
    13261 gtaaaaaaga gacctcaaaa gacactgcgc gtgtgctagg tggtatgtat gatggtattg
    13321 agtatcgtgg attttctcaa gaaacagttg agactttagc cgaattttca ggagttcctg
    13381 tgtggaatgg tttaaccgat gctgatcatc caacgcaagt actagccgat tttctgaccg
    13441 ctaaggaatg cttacataag ccctataaag atattcgctt tacgtatgtg ggtgatggac
    13501 gtaacaatgt cgctaatgcc ttgatgatcg gtgcttctat tgtaggcatg acttatcatt
    13561 tggtttgtcc taaagaatta gagccagatc ctgaactact ctctaaatgt caagaaattg
    13621 cgaaaactac tggtgcttct attgaaatca ctgctgatat tgcagaaggc gtcagagatt
    13681 cagacgtcct atatacagat gtttgggttt caatgggaga accggatgaa gtttggaaag
    13741 aaagaatagc tcttttagaa ccctatcgta ttacccaaga aatgcttaat atgacagaaa
    13801 atccaaacgt tattttcgag cattgcttgc cctcatttca taatatagat actaaagttg
    13861 gatacgacat ttatgaaaaa tatggtttga aggaaatgga agtatcagat gaagtttttg
    13921 aaggccctca ctcagttgtt ttccaagaag ctgaaaatcg tatgcatact attaaagcag
    13981 ttatggtagc aactttaggt gattaggagg aattttcatg gcaaaaattg ttgttgcact
    14041 aggaggaaat gccttaggaa attctccaga agaacaatta agattagtaa aacatactgc
    14101 aaaatcattg gtggccttga taaaaaaggg acacgaaatt gttgtaagtc atggtaatgg
    14161 accacaagtt ggtgccatta atttaggaat gaattttgcg gctgagagtg gtcaaggaac
    14221 taattttcct tttccagaat gtggagcaat gagccaagga tacataggct atcatctcca
    14281 acagagcctt ttaaatgaat tacgtcaaga gggaataaat aaggaagttg ctacaattat
    14341 tacccaaatt gaggtggatg aatcagacca agcatttagc gcacctacca agcctatagg
    14401 caccttttat gataaggaga catcagaaaa gattgctata gagaaagggt atactttcgt
    14461 agaagacgct ggacgagggt atcgtcgtgt tgttgcttca cctgaaccta aaaaaatcat
    14521 cgaaattaat agcattaaaa cacttattga aaatgatacc ctagttatcg caggtggagg
    14581 tggaggcatc cctgtcatta ataagggagg ttatgaaggt attgcagctg ttattgataa
    14641 agacaaatct agtgcactgt tagcaggaga actagctgcg gatcaattga ttattctcac
    14701 tgcagttgat tatgtttata ctcaatttgg taaagaaaat caaaaagctt taacagaggt
    14761 taatgagaat caaatgattg attacgttaa ccaaggtgag tttgcaaaag gaagtatgtt
    14821 acctaaagta atagcctgta tgagtttctt agatcacaat ccgaaaggaa cagccttaat
    14881 cacctcatta aatggtttag aagatgcatt agatgggaaa ttaggaacac gtataactaa
    14941 gtaatctcag ataagtgcaa agaaatgatt atttaacctt catctttgca ctttttctga
    15001 tgaagtcaat ctatttttat tatagaggag gcgtgtcatg ccagaaaaaa tgacaaacag
    15061 tgaaaacaaa aggagtaaag gttttcgaat gccaggtgcc tttacaatat tattcatttt
    15121 aacaatcttt tcggttttgg ctacatggtg gatacctgct gggtcctatt caaaattaca
    15181 atttgataca gcatcatcca aactagtagt aacagatcca aatggtaaga cagttcatgt
    15241 tccagcaacg caaacacagt tggataagat gaatgtcaaa attaaaatta aagaatttac
    15301 ttctggtgct attagtaagc cagtttcagt tccaaatact tacaaacgtt tgaagcaaaa
    15361 tccagcaggc attggctccg tgacgactag tatggtaaat ggaaccattg aagctgttga
    15421 tattatggta tttatcatgg tacttggagg catgatagga gtagttagaa agagtggcgc
    15481 ttttgagtct ggtcttctag ctttaacaaa gaaaaccaaa ggtagagagt ttttattgat
    15541 atttttagtt tccttattga tggttttagg cggtactcta tgtggtattg aagaagaagc
    15601 agttgctttt tatcctattt tagttccaat ctttttagcc atgggttatg attctatcat
    15661 ttgtgtaggt gctattttct tagcaagttc tgttgggaca tcattttcaa caattaatcc
    15721 gttttcttct gttattgctt caaatgcagc tggtattagt ttcacagaag gcttatcatg
    15781 gcgtacagca gggtgtattg caggtgctat ttttgttgtg gtgtatctac attggtatgc
    15841 taaaaaaata aaagcgaatc cagagttttc atattcctat gaagatagag tagaatttaa
    15901 tgctaaatgg ggcatgacaa ctaaccatac accctcactc tttactatta gacaaaaaat
    15961 tattttaagt ttatttgtta tttcatttcc tttgatggtt tggggagtaa tgtcacaggg
    16021 atggtggttt ccaaccatgg cttcttcctt tttagctatt acaattatta ttatgttttt
    16081 aacagcaact ggggcaaatg gtattggaga aagggatgtt gttgatgagt ttgttaatgg
    16141 tgcatcaagc cttgttgggg tttcattaat cattggttta gcgcgtggta tcaatattat
    16201 tttgagccaa ggttatatat cagatacgat gctttacaca gcttcaaaat tagcttcaca
    16261 tgttagtggc tctgtcttta tcattgttat gatgtttatc tactttgtgt taggatttgt
    16321 tgttccatcc tcgtcgggtt tagcagtact ttcaatgcca attctagcac ctcttgcaga
    16381 tactgttggg attcctcgtt cagtagtagt aatggcttat caatttgggc agtatgcaat
    16441 gttgttttta gcacctactg gactagttat ggcaaccttg cagatgttag atatgaagta
    16501 ctcccattgg ctaaaatttg tgtggccggt tgttttattc ctcctaatat ttggtggcgg
    16561 tttgttagtt ctacaagttt taatacttta aagatttcag ctattagtct caaaaatatc
    16621 tgaagagata taataaattg agtttaagtt aaataaaaaa aacgataggt gtaatctatc
    16681 gtttttttta tttcctttct ctagtctttc ttcaattttg gaaaggtgag cgtaaactga
    16741 ctgccatcat caggtttaga atcaacccat attttagcac ggtgtattgt agcgatttgt
    16801 tttactaaag atagccctaa accaattcca gactgactat ccttggttcg agcagagtcc
    16861 acttgataga aacggttcca aattttactt tggtcatcaa ctgagatacc tagaccatta
    16921 tctttaatag agaagacaat ttgattatcg gactgtctta gactaataga aatatgattg
    16981 tttgtaaatt taatagcatt tgaaaataga ttatcaaaaa gccgtttgag tagtgtttgt
    17041 tgacctataa tccaaaggtc atcttcgatt tgagagtcaa ggtgtatagg ggtattatct
    17101 aataatctac tcgaatcgga tactagttgc ttcatcataa ttgagaaatt aagaggttct
    17161 aactgaattg ataatggatt ttctgcctta gtcaattcta gaagttgctc aactaatttt
    17221 ttcattgata gtgactgacg gtgtataacc tcaaaggatt cttttgcttc acttaaattt
    17281 tctgcgtagt tttttccgta ttcactttca gaaagtataa ctgttacagg agtccgtagt
    17341 tcatgagaaa catcattgtt aaattgtttt tcccgaacaa atgatgattc aatagaagcc
    17401 aacatagtat tgataacagc tgctagctca gttaattcat tttcgttatt tttagtgata
    17461 attcgcttag tatagtcttc attttgggta ataaattgag cggtttcagt aatttcttga
    17521 atcggtctaa aagtgcgctt taaaattaaa tagccaccaa cagttattat aatcaacata
    17581 agaggaagaa cgatacccaa actaaataac aattcagtta attgcttatc cagtctgtca
    17641 acagtacgga tagcacgtaa ccactgattt ttcccagaaa ttggaatatc aacatactga
    17701 aaggtacgat tctccatgga aaatagagat aatttctttt tatgtttatg ttgatggtct
    17761 aaatcccttt taaatccttt tggaaaacca gaatagataa cttttccatt ttggtcatag
    17821 actgaataaa atattccatc ttcaaaagcc tcataccgat gacctttagc taacgcttga
    17881 ctcatttgca cagctttagc ttctaaattt tttttagatt tgttttcagc aatgcttttt
    17941 gaaataaaaa atgaaccaat taatacaccg ataacaagga taaaaataaa agagctgtac
    18001 cagagggtaa tttgagtgat gatgctagac tttttagggt aatttatttt actttggaat
    18061 aacatatcct aaacctcgtt tagtttttat tagtgatcca tctcgattgt tatctagttt
    18121 tcgacgaagg tttttgataa ggacatcaat aatattggac tctccataat aatcataatc
    18181 ccaaacgtgt tcacgaattt gatgacgaga gaggacttgg tctctgtgac gtgccaaata
    18241 ctcaaggact tcgtattcct tagctgttaa atcaatcaaa ttgtcgtttc tccaaacttg
    18301 ctttgtagat aaattgattg ttatatcttg aatttgaata tcatcagaag atacttgtct
    18361 atttgcacgt cgtaacatag ctcgaattcg ggccatgagt tctccaaatt caaatggttt
    18421 tacaaggtaa tcatccgcac caaaatctag acctgcgata cgatcttcaa cggcatctcg
    18481 agcagtcaac ataagaatag atacttgact tcctttgttg cgaagcagtt ttaaaaaggt
    18541 gaagccgtcc atttttggca tcattacatc taagataatc acatcatatt ctgccactga
    18601 aacaaattgt aaggcttctt caccatttga agcactgtca acactgtagt gttgcttttt
    18661 taaaagtttt acaatactac ggttcaagtc aaactcatct tcaacaacaa ggattttcat
    18721 tttttggcct ccgtatggct tttttccatt atatcacaaa agggaatgga gtactgaggt
    18781 cctattccct ttaatacact agtattagtc gtgattttta tgagttgtat taagcttatt
    18841 tgcctttata aatgatgctt tatcactatc aaattgggca atcttaggag tatttccatc
    18901 aagtagtttt actttataaa ctagcttatt tgatttgtta ttcgcaagag aaatttcttt
    18961 tatttttgca tttttatatt ttgcttgaat agcttgtaat gcagtctgaa tatctttact
    19021 tggttgtttt aattgggcat cttttgaatt gagttgatta tctgatgttg ttgaaaggat
    19081 cttcccagaa ttagcatcaa ttttatattt tttagctatt ttattatcaa taactttgat
    19141 ttcataaaaa ggtgtatcac ctttggtatt taattcaatt tgtgaaataa agcctgagcg
    19201 agcttctttt ttggcagttt ctaaggcagt cttataatcg atttttgtcg agatattctt
    19261 taatgttttt agtgagtatt tttctctatg atcgtgatgt ttatcgtgtc cttcatgctt
    19321 atgttcaaaa gacatgtgtt ctgagtgatg gttatgcgct aatgcagctc cacttactcc
    19381 tatagctccc ccaataccaa gtagcgtaat ggaagctaac cctacgtata atttattgct
    19441 catgaatttt ttaaagttgt ttttatagtt tgtcatactg acttccttct ttcttttttc
    19501 aaaccttgga ttaggtttgt tacttctgag tatatagtcg taaaa
//
LOCUS       DBJYSH010000027        19538 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.027, whole genome shotgun sequence.
ACCESSION   DBJYSH010000027 DBJYSH010000000
VERSION     DBJYSH010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 19538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19538
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.027"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            <1..900
                     /gene="fbsB"
                     /locus_tag="ACUBV3_000769"
     CDS             <1..900
                     /gene="fbsB"
                     /locus_tag="ACUBV3_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding surface protein FbsB"
                     /protein_id="HHU3847288.1"
                     /translation="KLILREEDKYSFEDDEEEFGNELLSYNKLKNEVLPVNITTSTIL
                     KPFEQKKIVEDFNPYSNLDNLEIKKIRLNGSQKQKVEQEKTKSPTPQKEAVKEQTEQK
                     ISGKTQNVEKKSETVATPQQSSTAQTSVQQPAPVQPVVQESKASQEEINAAHDAISAY
                     KSTVNIANTAGVTTAEMTSLINTQTSNLSDVEKALGNNKVNNGAVNVLREDTSRLENM
                     IWNRAYQAIEEFNVARNTYNNQMKSETVPVDNDIEAILAGSQAKISHLDNRIGARHMD
                     QAFVASLLEVTEMSKSISSRIKE"
     gene            967..1512
                     /locus_tag="ACUBV3_000770"
     CDS             967..1512
                     /locus_tag="ACUBV3_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GA module-containing protein"
                     /protein_id="HHU3847289.1"
                     /translation="MKKITTLILASSLLLVATTSVKADDNFEVPTRYVKMSEKSKAFY
                     QRLQEKQRKTHTTVKTFNNSEIRHQLPLKQEKARNDIYNLGILISQESKGFIQRIDNA
                     YSLENVSDIVNEAQALYKRNYDLFEKIKSTRDKVQVLLASHQGNTDLKNFYAELDDMY
                     EHVYLNESRVEAINRNIQKYN"
     regulatory      1663..1752
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            1908..2474
                     /gene="thiW"
                     /locus_tag="ACUBV3_000771"
     CDS             1908..2474
                     /gene="thiW"
                     /locus_tag="ACUBV3_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HHU3847290.1"
                     /translation="MTNHKTKKLTVLAIMIALDVFLTPIFRIEGMAFMSSVVNIIAGV
                     IMGPIYALVMALVTAFIRILTQAGVASVAPLAILGAGPGAFLAGWFYRFYAKDWMSWV
                     GEFIGTGIIGALLSAPIMVWFWTWTADGNHEVLAKASAAQSIFLFMPRFIGATIIGGV
                     IALLLLQGLKRVPVFMTIQALFKGEKVK"
     gene            2471..3025
                     /locus_tag="ACUBV3_000772"
     CDS             2471..3025
                     /locus_tag="ACUBV3_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HHU3847291.1"
                     /translation="MKLGLKDVLLIALLAVVLGVVYFGAGYISNAFVPFVGPIAHEVI
                     YGIWFVAGPMALYILRKPGTAIVAELLAALIEVLIGSIYGPSVLVIGTLQGLGSELGF
                     TLFRYHNYKLPAFILSAILTSIFSFAWSFYANGLSAFSFSYNILMLIVRTVSSIIFFL
                     LTKNICDQLHRSGVLNAYGIHEGK"
     gene            3029..4315
                     /locus_tag="ACUBV3_000773"
     CDS             3029..4315
                     /locus_tag="ACUBV3_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HHU3847292.1"
                     /translation="MLSVEKLAYTHGDSHYLFDEVTFSLNPGERILISGYSGCGKSTL
                     ALLLSGLKESGKGQVLLNGSLIEPSDVGFLFQNPDLQFCMDTVAHELYFILENLQIEP
                     EQMQDRSEFVLAQVGLKGFQNRLIYTLSQGEKQRLALATIFLKSPKLIILDEAFANLD
                     QESASQLLQLVLNYQANNQSMLIVIDHLITYYQDIMDHYFWLEKWLIRVDFDYMLNRL
                     NVFELEKKSHNTGDKLLSIKDFQVKLSKNKFISYLDFDLASGERLCLDGPSGVGKSSF
                     FMGLLGLYRTKGKKQFTHRKQIPISFLFQNPLDQFIFSTVYDEIFQVCKDSNKARDIL
                     ETINLWDKKQFSPFQLSQGQQRRLAIGSILASDSKLLLLDEPTYGQDAYHANMITTLL
                     LSYCHKNHCGVIFTSHDPHLKECFATRFLEVSSCSI"
     gene            4303..4971
                     /locus_tag="ACUBV3_000774"
     CDS             4303..4971
                     /locus_tag="ACUBV3_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T family protein"
                     /protein_id="HHU3847293.1"
                     /translation="MFNLKNYHPAITFLCLLIPTFSFSFTLRLRTSLLFLIIVVTLQC
                     FVKVSLKTWAKVNLISFVMGLSLFLGTYFWGKLPHQFVLASLVACRPLIFMNVGLLFH
                     ASHSNYDFIESLYQTFKVPSHFAYGIFAVFNLLPLIKLQYQRNRLAFRLKNQVTWALS
                     PRLILSVLLKTIYWVEQLELAMLSKGFEVGKERTHASTYPVRFRDYSLLGMSILLAIG
                     MIFK"
     gene            5055..5735
                     /gene="tenA"
                     /locus_tag="ACUBV3_000775"
     CDS             5055..5735
                     /gene="tenA"
                     /locus_tag="ACUBV3_000775"
                     /EC_number="3.5.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050334 - thiaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HHU3847294.1"
                     /translation="MLKDEMIAKGTKYIQSIYQDPFIQGIVKGRLDHDVICHYLQADN
                     IYLGKFADIYALCLAKSDNLRDKQFFLEQVDFTLNRELADGEGPHQALAAYTNRSYQD
                     IIEKAVWYPSADHYIKHMYFHFYENGIAGALAAMSPCPWIYHQLAKKIIEENQFLNGN
                     PFNNWITFYANDTVEELMENYFRMMDYYAQNLSKEKQADLVDAFVKSCQHERRFFQMA
                     INQEKWED"
     gene            5737..6534
                     /gene="thiD"
                     /locus_tag="ACUBV3_000776"
     CDS             5737..6534
                     /gene="thiD"
                     /locus_tag="ACUBV3_000776"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HHU3847295.1"
                     /translation="MTYLAPVLTIAGTDPSGGAGIMADLKTFQARRTYGMAVVTSVVA
                     QNTCGVRGVQHIETAIIDQQLACVYDDIKPKAVKTGMLAERETISLVASYLKKYPQPY
                     VLDPVMVATSGHRLIDSDAVEALKEDLLPLATIITPNLPEAEVLVGYDLSDEVSIIKA
                     GYDIQKQYSVRNVLIKGGHLDGLAKDYLFLEKEGLITLSNQRINTIHTHGTGCTFAAV
                     VAAELAKGQSILNAVSTAKSFITSAIETAPELGLGNGPINHTSYQGD"
     gene            6536..7306
                     /gene="thiM"
                     /locus_tag="ACUBV3_000777"
     CDS             6536..7306
                     /gene="thiM"
                     /locus_tag="ACUBV3_000777"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HHU3847296.1"
                     /translation="MYLEQLKEVNPLTICITNNVVKNFTANGLLALGASPAMSECIED
                     LEDLLKVADALLINIGTLTKESWQLYQEAIKIANKNQVPVVLDPVAAGASRFRLEVSL
                     DLLKNYPISLLRGNGSEIAALVGEKQASKGADGGKVADLESIAVKANQVFDVPVVVTG
                     ETDAIAVRGEVRLLQNGSPLMPLVTGTGCLLGAVLAAFIGSSDRSDDLACLTEAMTVY
                     NVAGEIAEKVAKGKGVGSFQVAFLDALSQMKSEMIMDK"
     gene            7320..7991
                     /gene="thiE"
                     /locus_tag="ACUBV3_000778"
     CDS             7320..7991
                     /gene="thiE"
                     /locus_tag="ACUBV3_000778"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001904425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HHU3847297.1"
                     /translation="MKDTLKLYFVCGTVDCSRKNILTVVEEALQAGITLFQFREKGFT
                     ALQGKEKIAMAKQLQILCKQYQVPFIIDDDIDLVELIDADGLHIGQNDLPVDEARRRL
                     PDKIIGLSVSTMAEYQKSQLSVVDYIGIGPFNPTQSKADAKPAVGNRTTKAVREINQD
                     IPIVAIGGITSDFVHDIIESGADGIAVISAISKANHIVDATRQLRYEVEKALVNRQKH
                     SDVIK"
     gene            8118..9377
                     /locus_tag="ACUBV3_000779"
     CDS             8118..9377
                     /locus_tag="ACUBV3_000779"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HHU3847298.1"
                     /translation="MRKIIINGGKQLTGEVAVSGAKNSVVALIPATILADDVVVLDGV
                     PAISDVDSLVDIMETMGAKIKRYGETLEIDPRGVKDIPMPYGKINSLRASYYFYGSLL
                     GRYGQATLGLPGGCDLGPRPIDLHLKAFEAMGASVSYEGDSMRLATNGKPLQGANIYM
                     DTVSVGATINTMIAAAKANGRTVIENAAREPEIIDVATLLNNMGAHIRGAGTDVITIE
                     GVKSLHGTRHQVIPDRIEAGTYIAMAAAIGRGIKVTNVLYEHLESFIAKLDEMGVRMT
                     VEEDSIFVEEQEKLKAVSIKTSPYPGFATDLQQPLTPLLLTAEGNGSLLDTIYEKRVN
                     HVPELARMGANISTLGGKIVYSGPNQLSGAPVKATDLRAGAALVIAGLMAEGRTEITN
                     IEFILRGYSNIIEKLTSLGADIQLVEE"
     gene            9479..10033
                     /locus_tag="ACUBV3_000780"
     CDS             9479..10033
                     /locus_tag="ACUBV3_000780"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HHU3847299.1"
                     /translation="MDIWTNLGRFAFIETEHVNLRPVAYTDREAFWRIASKRTNLQFI
                     FPVQTSKKESDFLLVHSFMKEPLGVWAIEDKVSHKMFGVIRFENIDLSKKTAEIGYFL
                     KESSWGQGIMTECLKTLSFFAFQEFGMDKLIIVTHKENIASQKVALKAHFKQSRSFKG
                     SDRYTRRIRDYIEFQLTRGDYRYE"
     gene            10026..11309
                     /gene="spxR"
                     /locus_tag="ACUBV3_000781"
     CDS             10026..11309
                     /gene="spxR"
                     /locus_tag="ACUBV3_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS-HotDog domain-containing transcription
                     factor SpxR"
                     /protein_id="HHU3847300.1"
                     /translation="MSKHQEILEYLENLAVGKRVSVRSISNHLKVSDGTAYRAIKEAE
                     NRGIVETRPRSGTVRVAQKAKVNIEKLTYAEIARISDSQVVAGTEGLSKEFSKFSIGA
                     MTHRNIEKYLVQGGLLIVGDRDEIQHLALQHQNAILVTGGFNVSPSVCRLADKLQIPV
                     MVTHYDTFTVSTMINHTLSNAKIRTDLKTVEQVYQSQMDYGFLAQDDTVKEFNLLVKQ
                     TKNVRFPIVNQANVVVGVVSVQDILGKDKEVKLATVMSKNIIVAKPRMSLANISQKMI
                     FEDLNMMPVVSDDFELLGVITRRQAVENLSMSQGTALYTYSDQILSNLQIEDGHFSFL
                     VEPAMIDHTGSLTQGVLTEFLKEICIRVLTRKHQRSIVVKQMTLYFLQPVQIDEIIMV
                     TPTIISEKRREATLDLELKLENKIIAKAMIAVKIN"
     gene            11332..12192
                     /locus_tag="ACUBV3_000782"
     CDS             11332..12192
                     /locus_tag="ACUBV3_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HHU3847301.1"
                     /translation="MITLKSAREIEAMDRAGDFLASIHIGLRDIIKPGVDMWEVEEYV
                     RRRCKEENVLPLQIGVDGAVMDYPYATCCGLNDEVAHAFPRHYTLKQGDLLKVDMVLS
                     EPLDKSIVDVSSLNFDNVAQMKKYTETYSGGLADSCWAYAVGEVSQEVKDLMSVTREA
                     MYIGIEKAVIGNRIGDIGAAIQDYAESRGYGVVRDLVGHGVGPTMHEEPMVPNYGTAG
                     RGLRLREGMVLTIEPMINTGTWEIDTDMKTGWAHKTLDGGLSCQYEHQFVITKDGPVI
                     LTSQGEERTY"
     gene            12194..13114
                     /locus_tag="ACUBV3_000783"
     CDS             12194..13114
                     /locus_tag="ACUBV3_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HHU3847302.1"
                     /translation="MKLKKFFEDLLAKLEYRPIQVFMRHFQSAEMDLSAIAVAYYLLV
                     TAFPLLVIAANIFPYFHINVSDLLSLMQKNLPKNIYEPASRLAVDAFSKPSTGILGFA
                     SLTAFWTMSKSLTSLQKAINKAYGVDQHRDFVISRLVGVGTGLIILFLLTFVLIFSTF
                     SKPVLQIIVNMYDLGDTLTAWLLNLAQPVTFLTIFLGIGILYFILPNARIRKVRYVIP
                     GTLFSTFVIGFFSNLISQYVLNRVEKMVDIKTFGSVVIFILMLWFIFLAHIMILGAIL
                     NASVQEIATGKIESRRGDIMSLIQKSKEEK"
     gene            complement(13131..13586)
                     /locus_tag="ACUBV3_000784"
     CDS             complement(13131..13586)
                     /locus_tag="ACUBV3_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HHU3847303.1"
                     /translation="MVKLKSLLKKSIQNEVSLYLLFGLLTSLLYLVIRQGIFNFSQDA
                     PFSAIVANIIAILFAFFTNDRFVFKQTKIGQFQRLQTFVIARLGTLGLDLILAVIFVD
                     QFPSIIGQFVQHNLNKINTIESLVSQILIILLNYILSKFVIFKDKKRQL"
     regulatory      13657..13770
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF01054"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class II; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF01054"
     gene            13768..14277
                     /locus_tag="ACUBV3_000785"
     CDS             13768..14277
                     /locus_tag="ACUBV3_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HHU3847304.1"
                     /translation="MNTFTTRDYAHMAIVTAIYIVLTITPPFNAIAYGAYQFRVSEML
                     NFLAFYHRKYLFAVTLGCMISNLYSFGMIDVFVGGGSTLLFVYLGTILFKQYQKDYLF
                     NGLINKAFFFFSFFFAASMITVAVELKIVAGLPLLLTWLTTAVGELASLLVGAVLVDK
                     LSRHVDFTK"
     gene            14417..16375
                     /gene="ligA"
                     /locus_tag="ACUBV3_000786"
     CDS             14417..16375
                     /gene="ligA"
                     /locus_tag="ACUBV3_000786"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001873525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HHU3847305.1"
                     /translation="MENRMNELVSLLNQYAKEYYTQDNPTVSDSQYDQLYRELVELEE
                     QHPENILPNSPTHRVGGLVLEGFEKYQHEYPLYSLQDAFSKEELIAFDKRVKAEFPTA
                     SYMAELKIDGLSVSLTYVNGILQVGATRGDGNIGENITENLKRVHDIPLHLDQSLDIT
                     VRGECYLPKESFEAINIEKRANGEQEFANPRNAAAGTLRQLNTGIVAKRKLATFLYQE
                     ASPTQKETQDDVLKELESYGFSVNHHRLISSSMEKIWDFIQTIEKDRISLPYDIDGIV
                     IKVNSLAMQEELGFTVKAPRWAIAYKFPAEEKEAEILSVDWTVGRTGVVTPTANLTPV
                     QLAGTTVSRATLHNVDYIAEKDIRIGDTVVVYKAGDIIPAVLNVVMSKRNQQEVMLIP
                     KLCPSCGSELVHFEDEVALRCINPLCPNQIKERLAHFASRDAMNITGFGPSLVEKLFD
                     AHLIADVADIYRLSIEDLLTLDGIKEKSATKIYHAIQSSKENSAEKLLFGLGIRHVGS
                     KASRLLLEEFGNLRQLSQASQESIASIDGLGGVIAKSLHTFFEKEEVDKLLEELTSYN
                     VNFNYLGKRVSTDAQLSGLTVVLTGKLEKMTRNEAKEKLQNLGAKVTGSVSKKTDLIV
                     AGSDAGSKLTKAQDLGITIQDEDWLLNL"
     gene            16387..17406
                     /locus_tag="ACUBV3_000787"
     CDS             16387..17406
                     /locus_tag="ACUBV3_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HHU3847306.1"
                     /translation="MKKQKKARLIYNPTSGQEIMKKNVAEVLDILEGFGYETSAFQTT
                     PTKNSARDEAARAAQAGFDLIVAAGGDGTINEVVNGIAPLKRRPKMAIIPTGTTNDFA
                     RALKIPRGNPIEATKLIGKNQIVKMDIGQAQEDNYFINIAAAGSLTELTYSVPSQLKT
                     TFGYLAYLAKGVELLPRVRKVPVKITHDKGEFIGDASMIFVAITNSVGGFEQIAPDAK
                     LDDGKFTLILVKTANLIEIMHLIRLVLAGGKHINDKRVEYIKTSYLTIEPLSDERMMI
                     NLDGEYGGDAPITLANLKNHIRFFANTDEISDDALVLDKDELAIEAIAQKFANEVNDL
                     EGLGD"
     gene            17410..>19538
                     /gene="pulA"
                     /locus_tag="ACUBV3_000788"
     CDS             17410..>19538
                     /gene="pulA"
                     /locus_tag="ACUBV3_000788"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051060 - pullulanase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HHU3847307.1"
                     /translation="MDNTVIVHYHSRKKNYFNLSLWQWRDGSEGQDAHFSRFDSFGAV
                     AILKYPLPYFLSHYYVIVKDKNWHHKTIDYRIDCTKGGARKEVWIVDGDDNLYYSRQA
                     AVSSHHYSRRQPHAYDMAINSRAFDKKWGFDGWLGFEYSETKTAFRLWAPTAERVELI
                     LYHSTDETASVSKVLSMKRGTAVNYKNHKENTHGVWFTELEGNYNYQAYTYRVYYRRR
                     TFKITRDPYSIATTANGKRSIVIAPEALTPEGFKISHGKEAKWRLENPNQAVIYEMHV
                     RDFSISETSGVKTDYQGKFKGLHQKDTVNQHGDKTTFDYVQDLGVNYIQLQPIFDHHQ
                     TFDDDGHYAYNWGYDPENYNVPEASFSSNPHEPATRILELKSAIQAYHDAGIGVIMDV
                     VYNHTFSSTDSAFQLTVPDYYYRMNHNGTFQNGSGCGNETASEKEMCRKYIIDSVLYW
                     VKEYNIDGFRFDLMGLHDVETMNIIRNELNKIDPRILVYGEGWDMGAGLTPQNKAKKD
                     NAYQMPGIGFFNDDVRDAVKGAEIYGEFKKGLVSGNSTEDIVAKGILGSDELVSYIDP
                     SQVLNYVEAHDNYNLNDLLWELHPNDNEKQHIYRVEVASAMNLLMQGMAFMQLGQEFL
                     RTKCYPTGDKGQLTQADKERAMNSYNAPDQVNQVNWDNVTFHKSTINFIRKIITLKTN
                     SPYFSYSSFEEIRKHVFVESAQYHSGF"
BASE COUNT         6353 a         2948 c         3981 g         6256 t
ORIGIN      
        1 aaattaattt taagggaaga agataaatat agttttgaag atgatgaaga agagtttgga
       61 aatgaacttc taagttacaa taagcttaag aatgaagttt tacctgttaa tattacaact
      121 tctactatat taaaaccgtt tgaacagaag aaaattgtgg aagattttaa tccttattct
      181 aatttagaca atttagaaat aaaaaaaata aggttgaatg gctcccaaaa acaaaaagta
      241 gaacaggaaa aaactaaatc gccaactcct caaaaagagg ctgtaaaaga acaaactgag
      301 caaaaaatat ctgggaagac tcaaaacgta gaaaagaaat ctgaaactgt ggcaactcca
      361 caacaaagtt caactgcgca aacttctgtc caacagccag ctccggttca accagttgtt
      421 caagaatcta aagcttctca agaggagatt aatgcagcac atgatgccat ttcagcgtat
      481 aaatcaacag tcaatattgc taatacagcc ggtgtaacaa ctgcggaaat gacctcgctc
      541 attaatactc aaacttctaa tctttctgat gttgagaaag ctttagggaa taataaggtt
      601 aataatggtg cagtcaatgt attgagagaa gatacatctc gtcttgagaa tatgatttgg
      661 aatcgtgctt accaagctat tgaagaattc aacgtcgctc gtaatactta taataaccaa
      721 atgaagtcag aaacagttcc agttgataat gatattgaag ctattttagc aggttctcaa
      781 gctaaaatta gccatttgga caatcgtatc ggagcgcgcc acatggatca agcttttgta
      841 gctagtttat tagaagttac tgagatgagt aaatcaatct catcgcgtat aaaagaatag
      901 acactgctat caaggcgatc ttaaactttt gtattgagct aacctaaaag atagaaagag
      961 actaatatga aaaaaataac aactttaatc ttagctagta gcttattact agttgcaacg
     1021 acatcggtta aagctgatga taactttgaa gtaccaacgc gttatgttaa aatgagtgaa
     1081 aaatcaaaag cattttatca aagattacaa gaaaaacaac gtaaaacaca tactactgtg
     1141 aagactttta ataattcaga aataaggcat caactacctc ttaaacaaga aaaggctagg
     1201 aatgatatct acaatttagg cattcttatt tctcaggagt ctaaagggtt catccaacgt
     1261 attgataatg cctattcttt ggaaaatgtc tcagatattg ttaatgaagc tcaggctttg
     1321 tataaacgca actatgattt atttgaaaaa atcaaatcta cacgtgataa ggttcaagtc
     1381 ttacttgcat cgcatcaagg taatacagac ttaaaaaact tttatgctga gttggacgat
     1441 atgtatgaac atgtttatct caacgaaagt agagtggagg cgataaacag aaatatccaa
     1501 aaatataatt agtttctaaa ctaacaaaca ttcctaaata taagacatta aaccctactt
     1561 attgattagt gagtagggtt ttactgtttt aaatagcttt ctgctcagaa tgtaagcctt
     1621 gtcatttcaa aggaactatg ttattattct taagtaaatt aaataggaca tttggggtgc
     1681 gtaacagctg agattatacc cattgaactt gatacagtta gtactgtcga agggaaatgt
     1741 gtgccattac aagttttttg taatggaaaa gggcgtagtg tcttcttagg agacagctag
     1801 ctctagttaa tcagataata aactccgttt gcttgcagat ggagtttttt gtcgtcaaaa
     1861 ttttttatag gttgttgccc cttatctgtc atagagaagg agttatgatg actaatcata
     1921 aaacaaaaaa attaacggtt ttagcaatta tgattgcttt agatgttttt ctaacgccca
     1981 tttttagaat agaaggcatg gcctttatgt ctagtgtggt taatattatc gctggagtta
     2041 taatgggacc aatctatgca ttagttatgg cacttgtaac ggcttttatt cgtattttaa
     2101 ctcaagcagg agtagcatct gttgcacctt tagctattct aggagctggt ccaggagctt
     2161 tcttagcggg ttggttttat cggttttatg cgaaagactg gatgtcttgg gtgggtgagt
     2221 ttataggaac agggataatt ggagcacttt tatcagctcc aattatggtc tggttttgga
     2281 cttggacagc ggatggaaat cacgaagtgt tagctaaagc atctgcagct caatccattt
     2341 ttctttttat gccaaggttt ataggggcga ctataattgg cggtgttatt gctttgttgc
     2401 tacttcaggg attaaagcgg gttcctgtat ttatgacaat acaagcatta tttaaaggag
     2461 aaaaagtaaa atgaaattgg gtttaaaaga tgtattattg attgctttat tagcggttgt
     2521 tctgggcgtt gtttactttg gagcaggcta tatttcaaat gcttttgtac cttttgtagg
     2581 tcctatagcg catgaagtta tttatggtat ttggtttgtg gcaggtccaa tggctcttta
     2641 tatattgagg aaaccaggaa ccgctattgt agcagaattg ttagctgctt tgatagaggt
     2701 tttaattgga tcaatttatg gaccaagtgt actcgttatt ggtacccttc aaggtttagg
     2761 gtcagaatta ggctttacgc tgtttaggta tcataattac aagttgccag cttttatatt
     2821 gtctgctatt ttgacatcaa tttttagttt tgcatggagt ttttatgcca atggacttag
     2881 tgcattttca ttttcatata acattctgat gcttatagta agaactgttt catctattat
     2941 tttcttctta ttaacaaaga atatatgtga ccaattgcat agaagtggtg tactgaatgc
     3001 ttatgggatt catgaaggga agtagcctat gctatctgtt gaaaaattag cttacaccca
     3061 tggcgatagt cattatttat ttgatgaagt gacttttagt cttaacccag gagaacgaat
     3121 tttaattagt ggctattcag ggtgtggtaa gagtacattg gctcttttac tatcagggtt
     3181 aaaagaaagt gggaaaggtc aagttttatt aaacggctca ttaatagaac caagcgatgt
     3241 gggttttctt tttcaaaatc ctgatttgca attttgtatg gatactgtgg cgcatgagtt
     3301 gtattttatt ttagaaaatt tacaaataga accggagcaa atgcaagata gaagtgagtt
     3361 tgtattggca caagttggtt taaaaggatt tcagaatcgt ttgatttata ctttatctca
     3421 aggtgaaaaa cagcgattag cattggcgac tattttttta aaatctccta aacttattat
     3481 tttagatgaa gcttttgcga atttggatca agagagtgcc agtcaactat tacaattagt
     3541 tcttaattat caagcaaaca atcaatcaat gctgattgta atcgatcatt tgattactta
     3601 ttatcaagat ataatggatc actatttttg gttggaaaaa tggttgataa gggtcgattt
     3661 tgattatatg ctcaatcgtt tgaatgtctt cgaattagaa aagaaaagtc ataatacagg
     3721 tgacaaactg ttatcaatta aagatttcca agttaaactt tcaaaaaaca aattcatctc
     3781 ttaccttgat tttgatttag caagtgggga gaggttgtgc ttagatggac caagtggcgt
     3841 ggggaagtct agtttcttta tgggattgtt agggctctat aggactaaag gaaagaaaca
     3901 atttactcat agaaagcaaa ttcctataag ctttttattt caaaatccct tagatcaatt
     3961 tattttttca acagtttatg acgaaatttt tcaggtttgc aaggatagta acaaagcgag
     4021 ggatattttg gaaaccataa atctttggga taaaaaacag ttttcaccat ttcaactaag
     4081 tcaaggccaa cagaggcgat tagccattgg cagtatttta gctagtgata gtaaacttct
     4141 attattagat gaaccaactt atggacaaga tgcctatcac gctaatatga ttacgactct
     4201 tcttttgagt tattgtcaca aaaatcattg tggggttatt tttactagcc atgatcctca
     4261 cttaaaagag tgttttgcaa cacgtttctt ggaggtgtca tcttgttcaa tttgaaaaac
     4321 tatcatccag ctatcacctt cctatgttta ttaataccaa ccttttcgtt tagtttcaca
     4381 ttgaggctaa gaacgtcttt gttatttcta attatagtag taacactgca atgcttcgta
     4441 aaggtttctt taaaaacttg ggcgaaggtc aatttgatct catttgtgat gggactctct
     4501 ttatttttag ggacgtattt ttgggggaaa ttaccccatc aattcgtctt agctagtcta
     4561 gtggcctgtc gtccgcttat ttttatgaac gtgggtttgt tatttcatgc atcacattct
     4621 aattatgatt ttatagaatc tctttatcaa actttcaaag tgcctagtca ttttgcatat
     4681 ggtatttttg cggtctttaa tcttttacca ttaattaaat tacaatacca acgtaatcga
     4741 ctcgcattcc gactgaagaa tcaggtaact tgggcattat cgcctagact aatattgtca
     4801 gttttattaa agacgatata ttgggtagaa cagttagagt tagcaatgct ttctaaaggt
     4861 tttgaagtgg gtaaagagcg aacacatgct tcaacttatc ccgttcgttt tcgagattat
     4921 agtttacttg gtatgagtat tttattggct ataggaatga tttttaaata agtacaatta
     4981 gtttagtaat acaattgtgc atttatatag agtgagcatg ttctgctcat gagatcataa
     5041 caaaggagat tactatgtta aaagatgaga tgattgctaa aggaacaaaa tatatacaat
     5101 ccatctatca agatccattt attcaaggaa ttgtaaaagg gcgtttagac catgacgtaa
     5161 tctgccatta tttacaggca gataatatct accttgggaa atttgctgat atttatgccc
     5221 tgtgcttggc aaaatctgat aatttacgag ataaacaatt tttccttgaa caagttgact
     5281 ttacattaaa tcgggagtta gcagatggtg aaggtccaca tcaagccttg gcagcttata
     5341 ctaatcgatc ttatcaggat attatcgaaa aggctgtatg gtacccgtca gcagatcatt
     5401 acattaaaca tatgtatttt catttttacg aaaatggtat tgctggtgct ttagctgcaa
     5461 tgagcccatg tccatggatt taccatcaat tggctaaaaa aataattgag gagaaccagt
     5521 ttttgaatgg taaccctttt aataattgga taacttttta tgctaacgat acagttgaag
     5581 agttaatgga aaactatttt cgtatgatgg attattatgc gcagaatttg tcaaaagaga
     5641 agcaagctga tttggtagat gcttttgtga aatcttgtca gcatgagaga cgtttttttc
     5701 aaatggcaat aaatcaagaa aaatgggagg attaatatga cttatttagc accagtttta
     5761 actattgcag gaactgatcc tagcggtgga gcagggatta tggcagattt aaagacattt
     5821 caagctcgtc ggacttacgg gatggctgtt gtgactagtg ttgttgctca gaacacttgt
     5881 ggggttagag gcgttcagca tatagaaaca gctatcattg atcaacaact agcttgtgtt
     5941 tatgatgata ttaagccaaa ggcggtcaaa acaggaatgt tagcggagag ggaaacgatt
     6001 agtttagtcg cttcatattt aaaaaaatat ccgcaacctt atgtattaga tcctgttatg
     6061 gtggcaacta gcggacatcg tttaattgat tcagatgccg tagaagcatt aaaagaagac
     6121 ttattgccat tagcaactat tatcacacca aatttaccag aagctgaggt tttagtggga
     6181 tatgacttat cagatgaagt aagtattatt aaagcaggtt acgatattca aaaacaatat
     6241 tctgttagaa atgttcttat aaagggagga catctagatg gtttggctaa agattattta
     6301 tttttggaaa aagaagggtt gataactctt agtaatcaac gtatcaatac tattcatact
     6361 catgggactg ggtgtacatt tgcagcagtc gttgcagcgg agttagctaa agggcaatca
     6421 attctaaacg cggtaagtac cgctaaatca tttattactt cagctatcga aacagcgcct
     6481 gagttgggtc ttggaaacgg tcctatcaat catactagct atcaaggaga ctaaaatgta
     6541 tttagaacaa ctaaaagagg taaatccttt aaccatttgt atcactaata atgtggtcaa
     6601 aaactttaca gccaatggtt tattagcttt aggagcttct ccggctatga gcgagtgcat
     6661 agaggactta gaagacttgt taaaagttgc agatgcatta ttaattaata taggaacact
     6721 aacaaaagaa agttggcaac tttaccaaga agcaatcaaa atagcaaata aaaaccaagt
     6781 tccagttgta ttagatcctg tagcggcagg agcaagtcgt tttcgattag aagtgtcact
     6841 tgatttattg aagaattatc ctattagttt acttagagga aatggtagtg aaatcgctgc
     6901 tttggttggt gaaaaacaag cgtctaaagg ggcagatggg ggaaaagtgg ctgatttaga
     6961 atcaattgct gtaaaagcca atcaagtttt tgatgtgcca gtggttgtca caggagaaac
     7021 tgatgctatc gctgttagag gagaagttcg cttattacaa aatggtagtc ctttaatgcc
     7081 acttgttaca ggaactggat gtctattagg ggcagtattg gctgctttta ttggtagttc
     7141 tgacagaagc gatgatcttg cttgtctgac agaggccatg actgtttata atgttgctgg
     7201 agaaattgca gagaaagtgg caaaaggtaa gggagtggga agtttccaag ttgcattttt
     7261 agatgccctt tctcagatga aatcagaaat gattatggac aaataggcgg tgtcagtaga
     7321 tgaaagatac attgaaatta tattttgttt gtgggactgt ggattgctct aggaagaata
     7381 tattgactgt tgttgaagaa gccttacaag caggtataac tctatttcaa tttagagaaa
     7441 aaggttttac agcattacaa ggtaaggaaa aaattgctat ggctaagcaa ctccaaatac
     7501 tttgtaagca atatcaagtt ccttttatta ttgatgatga cattgactta gttgagttaa
     7561 ttgatgctga tggtcttcat attggacaaa atgatttacc tgttgatgag gcacggagac
     7621 gtttaccaga taaaatcatc ggtttatctg tgagtacaat ggctgaatat cagaaatcac
     7681 aattatctgt tgttgattac atcggaattg gtccttttaa tccgacacaa tcaaaagcag
     7741 atgctaagcc tgctgttggc aataggacaa caaaggcagt tagagaaatt aatcaagata
     7801 ttccaatcgt tgcaataggc ggaatcacta gtgattttgt tcatgatatt atagaatcag
     7861 gcgcagatgg gattgctgtt atatcggcta tttcaaaagc aaatcatatt gtagatgcaa
     7921 ccagacaatt gcgctatgaa gttgaaaaag ctctggttaa taggcaaaaa cacagtgatg
     7981 taatcaaata aggctacatc gttaattata agaattttta agatattata tcatcacaca
     8041 atactggtat ttgaaaaaga atttaaacta tgataatata gtagagttga attctagtta
     8101 aagaaagatt tgaaagtatg cgtaaaatta ttattaacgg tggcaagcaa ctgacgggag
     8161 aagttgctgt atcgggtgcc aaaaatagtg tagttgcctt gattcctgcc actattcttg
     8221 ctgatgatgt tgtggtctta gatggagttc cggctatttc tgatgttgac agtttagttg
     8281 acattatgga aactatggga gctaagatta agcgttatgg tgagacgctt gaaattgatc
     8341 cacgtggcgt taaagatatt ccaatgcctt atggcaaaat caatagtctt cgtgcatcct
     8401 attatttcta tggaagctta ttggggcgtt atggtcaagc aacattaggt ttacctggtg
     8461 gttgtgattt aggcccccgt ccgattgatt tacatttaaa agcttttgaa gcgatgggag
     8521 ctagtgtttc ttatgaaggt gattcaatgc gtctagcaac aaacggaaag cctttgcaag
     8581 gagcaaatat ttatatggat actgtcagtg ttggtgcgac aattaacact atgatcgcag
     8641 cagctaaggc aaatggtcgt acggttattg aaaatgctgc gcgtgaacca gaaattattg
     8701 acgtggctac tcttttaaat aatatgggag ctcatattcg tggtgctggg actgatgtta
     8761 ttacaattga aggtgtgaaa tcgctccatg gcacgcgtca tcaagtaatt ccagatcgta
     8821 ttgaagcagg aacttatatt gctatggcag cagctatcgg acgaggaata aaagtgacca
     8881 atgtgttata tgaacacctt gagagtttca ttgctaagtt agatgagatg ggagttcgaa
     8941 tgacagttga agaggattca atttttgtcg aagaacaaga aaagttaaaa gctgtatcca
     9001 taaaaacctc accttaccca ggatttgcta cagatttaca acaacctcta acccctttat
     9061 tactaacagc agaaggaaac gggagtttat tagatactat ctatgagaaa cgtgttaatc
     9121 atgttcccga attagcacgt atgggagcta atatttctac cctagggggc aaaatagttt
     9181 atagtggtcc taatcaatta tctggtgcac ctgtaaaagc gacagatttg cgtgcaggcg
     9241 ctgctttagt tatcgctggc ttaatggcag agggacgaac tgaaattacg aatatcgagt
     9301 ttattttacg tggatattct aatatcatcg agaaactaac atcattgggt gcggatatcc
     9361 aattggttga ggaatagtat atataacaaa gagttgagaa tatttcttag ctcttttctt
     9421 cttgttttag attaagtcac ttataggtat ttgaaatctc ttgtggtata atattatcat
     9481 ggatatatgg acaaatttag gacggtttgc attcattgaa acagaacatg ttaacctacg
     9541 tcctgttgcc tacactgata gggaagcttt ttggcgaata gcttcaaaaa ggacaaattt
     9601 acaatttatt tttcctgttc aaaccagtaa gaaggaaagt gattttcttt tagttcattc
     9661 ttttatgaaa gagccgttgg gagtttgggc gattgaagat aaagttagtc ataaaatgtt
     9721 tggagttatc cgttttgaaa acattgattt atctaaaaag acagctgaaa ttggctattt
     9781 tttgaaagaa agttcttggg gtcaaggcat tatgactgaa tgtctaaaga cactttcttt
     9841 ttttgctttt caagagtttg ggatggacaa attgatcata gtaacacata aagagaatat
     9901 tgctagtcag aaggttgctt tgaaggcgca tttcaaacaa tctaggagtt ttaaaggaag
     9961 cgaccgttat acacgccgta taagagacta tattgagttt caattaacta ggggtgatta
    10021 tcgttatgag taaacatcaa gaaattttgg agtacctaga aaatttagct gttggtaaga
    10081 gggttagtgt acgcagtatt tcaaatcatt taaaggtgag tgatggcact gcataccgcg
    10141 ctataaaaga ggcagaaaat aggggtattg ttgagactag accgcgtagt gggacagtac
    10201 gtgttgctca aaaagcaaag gttaatattg aaaaactgac ttatgctgaa attgcaagga
    10261 ttagtgactc acaagttgtt gcaggaacag aggggctcag caaggagttt agtaaatttt
    10321 ctataggtgc aatgactcac agaaatattg aaaaatattt ggtgcaagga gggttattga
    10381 ttgttggcga ccgtgatgaa atacaacatt tagccttaca acatcaaaac gctatcttag
    10441 ttacaggtgg ctttaatgtt tccccatctg tttgtcgttt agcagataaa cttcaaattc
    10501 ccgtaatggt aacacactat gatacattca cagtatcaac gatgattaat cacactttgt
    10561 caaatgccaa aattcgtact gatttaaaga ccgttgaaca agtctatcag tcgcaaatgg
    10621 attacggttt cttagcgcag gatgataccg taaaagaatt taatcttctt gttaaacaaa
    10681 ctaaaaatgt tcgttttcca atagtcaacc aagctaatgt tgtcgttggt gttgttagtg
    10741 tgcaagatat tcttggaaaa gataaagaag taaaattggc aacagtcatg tcaaaaaata
    10801 tcattgttgc taaacctcga atgagtctag ctaatatcag tcaaaaaatg atttttgaag
    10861 atttgaatat gatgccagta gtatcggatg actttgaatt attaggcgtt attacaagac
    10921 gacaagctgt agaaaattta tcgatgtcgc aagggacagc tttatacacc tatagtgatc
    10981 aaatcttgtc taatttacaa attgaagatg gtcacttttc gtttttagta gaacccgcta
    11041 tgattgacca tacaggtagc ttaacacaag gagttttgac ggagttctta aaagaaattt
    11101 gtattcgtgt cttaactcgt aagcatcaac gcagtattgt tgttaaacaa atgacacttt
    11161 atttcttgca acctgttcaa attgatgaaa taataatggt aacgccaaca attatttcag
    11221 aaaaacggcg cgaagctaca ctcgatttag aattaaaatt agaaaataaa attattgcta
    11281 aagctatgat agcggtaaaa attaattaga tataaggaga ataaatagaa aatgattaca
    11341 ttaaagtcag cacgcgaaat tgaagcaatg gatagagcag gtgatttttt agccagcatc
    11401 cacattggac ttcgtgatat tatcaaacca ggagttgata tgtgggaagt tgaagaatac
    11461 gttcgtagac gttgtaaaga agaaaacgta ttgccattgc aaatcggggt tgatggtgca
    11521 gttatggatt acccatatgc tacttgttgt gggcttaatg atgaagtggc tcatgccttt
    11581 cctcgtcatt ataccctaaa acaaggtgac cttttaaaag tagatatggt tttaagcgaa
    11641 cctttagata aatcaattgt tgatgtttca tcgttaaact ttgacaacgt ggctcaaatg
    11701 aaaaagtaca cggaaacata tagtggtggt ttggcagact catgttgggc ttatgctgta
    11761 ggtgaagttt cacaagaggt gaaagactta atgtctgtga caagagaagc aatgtatatt
    11821 gggattgaaa aagcagttat cggaaaccgt attggtgata ttggagctgc tattcaggat
    11881 tatgcagaga gtcgtggtta tggagttgtt cgtgatttgg ttggtcacgg tgttggtcca
    11941 acaatgcatg aagagcctat ggtgcctaac tatggtacag caggtcgagg tctccgtctt
    12001 cgtgagggga tggttttaac catcgaacca atgattaata ctggtacttg ggaaattgat
    12061 acagacatga aaactggttg ggcacataaa accttagatg gtggtctttc ttgtcaatat
    12121 gaacatcaat ttgttatcac taaagatggt cctgttattt taacgagtca aggagaagaa
    12181 agaacttact aaaatgaaat taaaaaaatt ctttgaggat ttgctggcta aactagaata
    12241 tagacccatt caagttttta tgcgtcattt tcaaagtgca gagatggact tatcagccat
    12301 agcagttgct tattatctat tagtgacagc ttttccatta ctagttattg ctgctaatat
    12361 ttttccttat tttcatatta atgtgtcgga tttattgtca cttatgcaaa aaaatcttcc
    12421 gaaaaatatt tatgaacctg cctcacgctt agcagtagat gctttttcga aaccgtctac
    12481 cggcattttg ggattcgcgt cattaacagc tttttggaca atgtctaaaa gtttgacctc
    12541 tcttcaaaaa gccattaata aagcatacgg tgttgaccaa cacagagact ttgtgattag
    12601 tcgtttagtg ggtgtgggga ctgggctgat catcctattt ttactgactt ttgttttaat
    12661 tttttcaacc ttttcaaaac cagttttaca gataattgta aacatgtatg atttgggaga
    12721 tactctaacg gcgtggcttt taaatcttgc acaaccagtt acatttttga ctatattttt
    12781 aggtattgga attttatact ttattttacc taacgcacga attagaaaag tacgttatgt
    12841 cattccaggg acattatttt caacgtttgt tattggtttt tttagtaatt taattagcca
    12901 atacgtctta aatagggttg aaaaaatggt agatattaaa acgtttggtt cggtcgttat
    12961 ctttatctta atgctatggt ttattttctt agcccacatt atgattttag gtgcaatact
    13021 gaatgccagc gttcaagaaa ttgcgacagg aaaaattgaa tcaaggcgtg gagatattat
    13081 gtctcttatc caaaaatcca aagaagaaaa ataagaaaag acagctcttg ttagagttgc
    13141 ctttttttgt ctttaaaaat aacaaatttg ctcaagatgt agttgagtaa aataattaat
    13201 atttgagata caaggctttc aatggtgttg attttattta aattgtgctg aacgaattgt
    13261 ccgataatag aaggaaattg atcaacaaaa ataacagcta atatcaaatc taatcctaat
    13321 gtgcctaatc tagcgatgac aaatgtttgt aagcgttgaa actgtccaat tttagtttgt
    13381 ttaaaaacaa atcgatcatt tgtaaaaaaa gcaaaaagta tagctattat gttagcaact
    13441 attgcagaga atggagcgtc ctgcgagaaa ttaaaaatcc cttgtcgaat aactaaataa
    13501 agaagggatg ttaatagtcc gaacagtaaa tataagctga cttcattttg aatacttttt
    13561 ttcagtaacg attttaattt aaccatattt ttattatatc acggatgctt caaagtgatg
    13621 aggaaatctc ccataagctt ggctttactt tttgtttgtg atatacttag taagttgttt
    13681 tacacaaccc ttgatgctta gttccctttc atcaagcata ttacaaacgt tagaagtgtt
    13741 aactgtctaa acgttaaagg agaaattatg aacacattta caactcgcga ttacgctcac
    13801 atggctattg taacagctat ctatatcgtt ttaaccataa cgccaccttt taatgctatt
    13861 gcttatggtg cttatcaatt ccgtgtttca gaaatgctaa atttcttggc cttttatcac
    13921 cgaaaatacc tttttgcggt aacgttagga tgtatgattt ctaatcttta tagttttgga
    13981 atgattgatg tctttgtagg tggtggttca actttacttt ttgtttatct tggaaccatt
    14041 ttattcaagc aatatcaaaa agattatcta tttaatggcc ttattaacaa agcattcttt
    14101 ttcttttctt tcttctttgc tgcttcaatg attacggtag cagttgaatt gaaaattgta
    14161 gcaggattac ctttattatt aacatggtta acgacagctg ttggtgaatt ggcttcactt
    14221 ttggttggag cagtccttgt ggataagttg agtagacacg tcgattttac aaaataaata
    14281 tattataaag cgcgagacat tgatctcggc ttttttgttt ggttttatag ttagcatagc
    14341 tagaaaatgc tgcaactttt atgagaaatg ctccaatttt tattaatatc cttagtcttt
    14401 gtgataaaat agaaatatgg aaaatcgtat gaatgaatta gtatctttgt tgaatcagta
    14461 cgcaaaagag tattatacac aagataatcc cacagtttct gacagtcaat atgaccagtt
    14521 atatcgagag ttagtagaat tagaagagca acatccagaa aacattttgc caaatagtcc
    14581 cacacatcga gtgggtggtt tagttttaga aggctttgaa aaataccaac atgaatatcc
    14641 tttgtattca ttgcaggatg ctttctcaaa agaagaattg atagcctttg ataaacgtgt
    14701 taaagcagag tttccaactg cttcttatat ggcagagctg aaaattgatg gtctatctgt
    14761 ctcgttgact tatgtaaatg gtattttaca ggttggggca acacgtggtg atggcaatat
    14821 tggagaaaat attactgaaa atttaaagcg tgttcatgat attcctctac atttagacca
    14881 aagtttagat attactgttc gcggagagtg ctaccttccc aaagaatctt ttgaagctat
    14941 taacatagaa aaacgtgcaa atggtgagca ggaatttgct aatcctagga atgcagcagc
    15001 aggaaccttg cgccaattaa acacagggat tgttgcgaaa cgtaaattag caacttttct
    15061 ttatcaagaa gctagcccta cgcaaaaaga aacacaagat gatgttttaa aagagctaga
    15121 aagctatggc ttttcagtca atcatcatcg tttaattagc tcttctatgg agaaaatttg
    15181 ggattttatc caaactatag aaaaagatag aatttccctc ccttatgata tcgatggtat
    15241 tgttattaag gttaatagct tagcaatgca agaagagttg ggcttcactg taaaggcacc
    15301 tcgttgggcg attgcatata aattccctgc tgaagaaaag gaagcagaaa tattatctgt
    15361 tgattggaca gttgggcgaa caggagttgt aacaccaaca gctaacctaa ctccagttca
    15421 actagcaggg acaaccgtta gtagggccac tctgcataat gtagattata ttgccgaaaa
    15481 agatatccgt attggagata ctgttgttgt atacaaagca ggggatatca tccctgcagt
    15541 cttaaatgtc gttatgagta agcgaaacca gcaagaagtc atgcttattc ctaaactatg
    15601 tccttcttgt ggtagtgaat tagttcattt tgaagatgag gtggctttac gttgtattaa
    15661 tccgctgtgc cccaaccaaa tcaaagaacg tttagctcat tttgctagcc gggatgctat
    15721 gaatattaca ggttttggtc catccttagt cgaaaaatta tttgatgctc atttaatagc
    15781 agatgttgca gatatttatc gtttaagtat agaggattta ttaactttag atggtattaa
    15841 agagaaatct gcaactaaga tttatcatgc aatccaatct tcaaaagaaa attctgctga
    15901 aaaacttctt tttggcctag gtattcgtca tgtgggaagc aaggcgagcc gccttttatt
    15961 ggaggaattt ggtaatttga ggcaactaag tcaagcaagt caagaaagta ttgcctcaat
    16021 tgatggacta ggaggggtta ttgccaaatc acttcatacg ttctttgaaa aagaagaggt
    16081 agataaacta ctagaggaat taacttccta taacgttaat tttaattatc taggaaaacg
    16141 agttagtaca gatgcacaac tttcaggttt aacagtcgtt ttaaccggaa aacttgaaaa
    16201 gatgactcgt aatgaagcaa aagagaaatt acaaaacctt ggtgctaagg tgacaggtag
    16261 cgtttctaaa aagactgatt taattgttgc aggaagcgat gcaggaagta aactaaccaa
    16321 ggctcaagat ttagggatta caatacaaga tgaagactgg ttactcaacc tttaagtgag
    16381 gaagaaatga agaaacaaaa aaaagcgcgt ttaatttata acccaacttc tggtcaagaa
    16441 attatgaaaa aaaatgtagc ggaagttctt gatatacttg aaggatttgg gtatgaaaca
    16501 tcagcctttc aaacaacgcc aactaaaaat tctgccagag atgaagctgc tagagctgct
    16561 caagctggtt ttgatttgat tgttgcagca ggtggagatg gtactattaa tgaagttgtt
    16621 aatggtattg ctcctttgaa acgtcgtcct aagatggcaa ttattccaac tggaacaact
    16681 aatgattttg ctcgtgcctt aaaaataccg cgaggtaatc ctattgaagc aactaaatta
    16741 attggtaaaa atcaaatcgt taagatggac attggtcaag ctcaagagga caattatttt
    16801 attaatattg cagctgcagg tagtttgaca gagttaactt atagtgtacc aagtcaattg
    16861 aaaacaacgt ttggctatct tgcttatcta gcaaaaggtg ttgaattatt acctcgagtt
    16921 cgaaaagtac cagtcaaaat tactcacgat aagggtgaat ttatcggtga tgcttcaatg
    16981 atttttgttg ccatcacaaa ttctgtagga gggtttgaac aaattgcacc tgatgccaaa
    17041 ttggatgatg ggaaatttac ccttatttta gtaaaaactg caaatcttat tgagattatg
    17101 catctcattc gtttggtgct agcaggtgga aaacatatta atgataaacg agtagagtat
    17161 attaaaacgt cttatctaac tatcgaaccc ctatcagacg agcgtatgat gattaactta
    17221 gatggagaat atggaggaga tgctcccatt actctagcaa accttaaaaa tcatattagg
    17281 ttctttgcta atactgatga aatttcggat gatgctttag ttttagataa agatgaatta
    17341 gcgatagaag ctattgcaca gaagtttgct aatgaggtaa atgatttaga aggattagga
    17401 gattgatgaa tggataatac tgtcattgta cattaccata gccgaaagaa aaattacttt
    17461 aatttaagtt tatggcagtg gcgcgatggc tcagagggtc aagatgctca tttttcacgg
    17521 tttgacagtt tcggtgctgt tgcgatacta aaatatccct tgccatattt tttaagccat
    17581 tattatgtga ttgttaagga taaaaattgg catcataaaa caatagatta tcgcattgat
    17641 tgtactaaag gtggtgcacg taaagaagtt tggattgtag atggggatga caatctgtat
    17701 tattctcgtc aagcagcagt tagtagccat cattatagta gacgccaacc tcatgcctac
    17761 gatatggcaa tcaatagtag agcgtttgat aaaaaatggg gatttgatgg ttggttaggt
    17821 tttgaatatt cagaaactaa aacggctttt agattgtggg cccctactgc ggaacgtgta
    17881 gaactgattt tatatcattc gacagatgaa acagcaagtg tatcaaaagt tctttctatg
    17941 aaaagaggga cagccgtcaa ttataaaaat cataaagaaa acacccatgg cgtctggttt
    18001 actgaacttg aaggaaacta taattaccaa gcatatactt atcgagtcta ctatagacga
    18061 cgtaccttta aaattacgag agacccttat tccattgcaa cgactgcaaa tggtaaaagg
    18121 tctattgtta ttgctccaga agcattaact ccggaagggt ttaaaataag tcatggaaaa
    18181 gaagctaagt ggcggctcga aaaccccaat caagcagtca tttacgaaat gcatgtgcgt
    18241 gatttttcga tatctgagac ttcaggagtt aagacagatt atcaaggaaa atttaaggga
    18301 ttgcatcaaa aagatacagt taatcagcat ggtgataaaa cgacctttga ttatgtgcaa
    18361 gacttaggag ttaactatat ccaacttcaa ccaattttcg atcatcatca gacgtttgat
    18421 gatgatggtc actatgctta taattgggga tatgatcctg aaaactacaa cgttccagaa
    18481 gcaagcttct ctagtaatcc acatgaacct gcaacgcgta ttttagaatt aaaatctgct
    18541 atccaagcgt accatgatgc aggcattggt gttattatgg atgttgtcta taatcatact
    18601 ttttcatcaa ctgattctgc atttcaacta acggttccag attattatta tcgaatgaat
    18661 cacaatggaa catttcaaaa tggttcaggc tgtggtaatg aaacagctag tgaaaaagaa
    18721 atgtgtcgta agtatattat tgattcagtt ttgtattggg tgaaagaata taatatcgat
    18781 ggttttcgat ttgatttgat gggactacat gatgtagaaa cgatgaacat tataagaaat
    18841 gagctaaata aaatcgaccc tcgtattttg gtttatggag aaggatggga catgggagct
    18901 ggtctcacgc ctcaaaataa agctaaaaaa gataatgctt atcagatgcc tggaattggc
    18961 tttttcaatg atgatgtaag agacgctgtc aaaggtgcgg aaatttacgg agaatttaaa
    19021 aaaggcttag tttctggaaa cagtacagaa gatattgttg caaaaggcat tttaggaagt
    19081 gatgagttag tatcatacat tgatcctagt caagttttaa attatgttga agcacatgac
    19141 aactacaact taaatgattt gctatgggaa ttacatccta atgacaacga gaaacaacat
    19201 atttaccgag ttgaagtagc tagtgctatg aatctattga tgcaagggat ggctttcatg
    19261 caattaggtc aggagtttct tcgaacaaag tgttatccta caggtgataa aggtcaactt
    19321 actcaagcag ataaagaaag agctatgaat tcttacaatg ccccagacca agttaatcag
    19381 gtcaactggg ataatgttac ctttcataag agtactatta attttattag gaaaataatt
    19441 actttgaaga caaactctcc ttacttttct tactctagct ttgaagagat tcgcaaacat
    19501 gtttttgtag agtctgctca ataccattca ggtttcat
//
LOCUS       DBJYSH010000028        19424 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.028, whole genome shotgun sequence.
ACCESSION   DBJYSH010000028 DBJYSH010000000
VERSION     DBJYSH010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 19424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19424
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.028"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            9..1010
                     /locus_tag="ACUBV3_000789"
     CDS             9..1010
                     /locus_tag="ACUBV3_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HHU3847308.1"
                     /translation="MSKKMTINDIAQLSKTSKTTVSFFLNQKFEKMSDETRQRIQEVI
                     DETGYRPSTIARSLNSKKTKLLGVLIGDITNTFSNQIVKGIEHITKQKGYQIIVGNSN
                     YDAKSEEDYIENMLNLGVDGFIIQPTSNFRKYSRILKEKKKPMVFFDSQLYEHKTSWV
                     KANNYDAVYDMTQECLNRGYKKFIMITGDTSLLSTRIERASGFMDALKDNGFGYDTLV
                     IEDDDHSKSDIEDFLKAVVPDKEETLVFAPNCWALPMVFTAMKNLNFDMPRVGLVGFD
                     NIEWTDFSSPKVSTIVQPAYEEGEQVAQILINRIEGDDSVDNQQIVDCQMFWKESTF"
     gene            complement(1081..1695)
                     /gene="def"
                     /locus_tag="ACUBV3_000790"
     CDS             complement(1081..1695)
                     /gene="def"
                     /locus_tag="ACUBV3_000790"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HHU3847309.1"
                     /translation="MSAIDKLVKASHLIDMNDIIREGNPTLRKVAEEVTFPLSEKEEI
                     LGEKMMQFLKHSQDPIMAEKLGLRGGVGLAAPQLDISKRIIAVLVPNVEDAQGNPPKE
                     AYSLQEVMYNPKVVSHSVQDAALSDGEGCLSVDREVPGYVVRHARVTIEYFDKTGEKH
                     RLKLKGYNSIVVQHEIDHIDGIMFYDRINEKNPFAVKEGLLILE"
     gene            complement(1761..2405)
                     /locus_tag="ACUBV3_000791"
     CDS             complement(1761..2405)
                     /locus_tag="ACUBV3_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HHU3847310.1"
                     /translation="MDIENYCQDYCLETIFPKHYFKDLQVITLTAGQSVCKQGEQLEY
                     LHYIVKGRFKIVRRLFNGKEHILDIKTKPTLIGDIELLTNRQIVSSVIALEDLTVIQL
                     SLKGRKEKLLTDATFLLKLSQELAQAFHDQNIKASTNLGYTVKELLASHILAIEEQGY
                     FQLELSSLADSFGVSYRHLLRVIHDMVKEGLIQKEKPKYFIKNRFALESLNIQP"
     gene            2517..3680
                     /locus_tag="ACUBV3_000792"
     CDS             2517..3680
                     /locus_tag="ACUBV3_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HHU3847311.1"
                     /translation="MKIFMEKLSLLSLSLILLSTFSTSPALPQMISYYRDKGLPSPQV
                     ELLFSIPSMAIIFILLITPWLSKKLSEKHMIIFGLLLTALGGGLPVVSQNYLLVFVSR
                     LLLGSGIGFINARAISVISEYYQGKERRKLLGLRGSFEVLGNAGLTALVGLLLTFGWS
                     KSFMIYFLALPILVLYLVFAPKKVVKDTNDKIKTKGQKIPKADLTYIVALAILAGFVI
                     TINTGINLRIPLLVVEFGLGTPAQASLVLSAMMLMGIIAGMSFGQLIAMFHKQLIPIC
                     LVLFSLTLLGVGLPSNLMVLTISAMASGFLYSLMVTAVFSLVADRVEYSLVGSATTLV
                     LVFCNIGGASAAILLSCFDHLLGQINAVFYVYAILSLAVGMIYFFKRLFFKKM"
     gene            complement(3726..4709)
                     /locus_tag="ACUBV3_000793"
     CDS             complement(3726..4709)
                     /locus_tag="ACUBV3_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HHU3847312.1"
                     /translation="MIEKYYNWATLGTGVIANELAQALEARGQKLYSVANRTYDKGLE
                     FATKYGIQKVYDHIDQVFEDPEVDIIYISTPHNTHISFLRKALANGKHVLCEKSITLN
                     STELKEAIDLAETNHVVLAEAMTIFHMPIYRQLKTLVDSGKLGPLKMIQMNFGSYKEY
                     DMTNRFFSRDLAGGALLDIGVYALSCIRWFMSEAPHNITSQVTFAPTGVDEQVGILLT
                     NPANEMATVSLSLHAKQPKRATIAYDKGYIELFEYPRGQKAVITYTEDGHQDILEAGK
                     TENALQYEVADMEEAVSGKTNHMYLNYTKDVMDIMTQLRQEWGFTYPEEEK"
     gene            4857..6752
                     /locus_tag="ACUBV3_000794"
     CDS             4857..6752
                     /locus_tag="ACUBV3_000794"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000599042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HHU3847313.1"
                     /translation="MIKYQDDFYQAVNGEWAKTAVIPDDKPRTGGFSDLADDIEALML
                     STTDKWLADENKPSDTILNHFIAFHKMTADYQKREEVGVSPVLPLIEEYKGLQSFSEF
                     ASKVAEYELEGKPNEFPFGVAPDFMNAQLNVLWAEAPGIILPDTTYYSEDNEKGKELL
                     AFWRKSQEDLLPLFGLSEQEIKDILDKVLALDAKLAQYVLSREESSEYVKLYHPYNWE
                     DFTKLAPELPLDAIFQKILGQKPDKVIVPEERFWTEFASDYYSESNWELLKADLILSA
                     ANAYNAYLTDDIRIKSGVYSRALSGTPQAMDKKKAAYYLASGPYNQALGLWYAGEKFS
                     PEAKADVEHKIATMIDVYKSRLEKADWLAQSTREKAIMKLNVITPHIGYPEKLPETYT
                     KKIIDPKLSLVENATNLDKISIAYGWSKWNKPVDRSEWHMPAHMVNAYYDPQQNQIVF
                     PAAILQEPFYALEQSSSANYGGIGAVIAHEISHAFDTNGASFDEHGSLNNWWTDEDFE
                     AFKKLTDKVVEQFDGLESYGAKVNGKLTVSENVADLGGVACALEAAQRESDFSARDFF
                     INFATIWRMKARDEYMQMLASVDVHAPAQWRTNITVTNFEEFHKEFDVKDGDNMWRPV
                     EKRVIIW"
     gene            complement(6802..7755)
                     /locus_tag="ACUBV3_000795"
     CDS             complement(6802..7755)
                     /locus_tag="ACUBV3_000795"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S66 family peptidase"
                     /protein_id="HHU3847314.1"
                     /translation="MKKLTKGSHIRVLSPSSSIERLGGFEANLLAKEVLEKLGFQVSF
                     SKHYLENDILYSASIASRVEDLHEAFADPSVDAILATIGGFNSNELLPYLDYDLISKN
                     PKIICGYSDSTAFLNAIFAKAKIQTYMGPAYSSFKMKEGQPYQTQAWLTAMTENHYEL
                     WPSEEWSSDPWYDPSKPRQFFPTEWKIYNHGKASGTIIGGNLSTFGLLRGTPYAPKIE
                     RYVLLIEEAEESNFYEFDRNLAAILQAYPHPQAILMGRFPKECGMTPQVFEYILSKHA
                     IFKEIPVIYDMDFAHTQPLLTVTIGAELSVDTTTLSLSIKE"
     gene            7922..8158
                     /locus_tag="ACUBV3_000796"
     CDS             7922..8158
                     /locus_tag="ACUBV3_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3847315.1"
                     /translation="MSQLSLEEQLNRLKDKYLSSKNQEFSNVHLSKVKKKKKKLIILE
                     KERCRCRLEREDTKKIDDKIEKLKKELIDVMKEE"
     gene            8160..10229
                     /locus_tag="ACUBV3_000797"
     CDS             8160..10229
                     /locus_tag="ACUBV3_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HHU3847316.1"
                     /translation="MEETILIVSFLLFLILSNVINRIFPKLPLPFIQLVFGILSGLVF
                     HKSQVHIDPELFLAFVIAPLNFREGQESDIGSFIKYRAIILYLILPTVFLTAIVVGYV
                     AGHLLPVSLPLAACFALGAALGPTDAVAFISIAKRFQFPKRAENILKLEGLLNDASGL
                     VSFQFALTALVTGYFSLAKASLKLALAIMGGFLIGLLFAFLMRLCLTVLEKFDAADVT
                     GALLLELTLPFVAYFVADLLGFSAIIAVVVAGVMQANRLKKVTLFDAQVDRVTSVIWE
                     TLNFILNGLVFLIFGRELTRIIGPLLTSNAYSNFDLISIVVLVTCTLFLVRFLAVSCF
                     YAWRSFKYHKSFKKYWREIQLLTFSGVKGSVSIATILLLPKHSVIGELGYSLILFTVG
                     AVTLMSFLTGLLVLPKLAPPLQVKDDYLIRLSILTKVLSVLEEDGKSSENQASFYAVI
                     DNYNSRIRHLILEQESSDIKKDLAELQLMMLSIESDGLEAAYRYGNISIKEYRIYQRY
                     LKYLERQVNRSFVSNITYSLTILMRVIRGIVHEFLTFGQGIRNRKKHFKNRKRLLAEV
                     NRQHLTDLYLSNNDLILETLEDMEGYYNATLIDFLQNERIRDAELIKSGLFVERVIAR
                     ITPDILNETLRGYYLERRTISEFERDGLISSKESQILRDNVNELESYSLRDSQSSIPY
                     QLLDLKK"
     gene            complement(10301..10371)
                     /locus_tag="ACUBV3_000798"
     tRNA            complement(10301..10371)
                     /locus_tag="ACUBV3_000798"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10337..10339),aa:Cys,seq:gca)
     gene            complement(10506..12038)
                     /gene="ahpF"
                     /locus_tag="ACUBV3_000799"
     CDS             complement(10506..12038)
                     /gene="ahpF"
                     /locus_tag="ACUBV3_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HHU3847317.1"
                     /translation="MVLDKEIKAQLAQYLDLLESDIVLQADLGDNDNSQKVKDFLDEI
                     VAMSDRISLESTHLKRQPSFGIAKKGHESRVIFSGLPMGHEFTSFILALLQVSGRAPK
                     VDEDIIKRIKGIEKTINLETYVSLTCHNCPDVVQAFNIMAVLNPNITHTMIEGGMYQD
                     EVKSKGIMSVPTVYKDQEEFTSGRATIEQLLEQLDGPLDAEAFADKGVYDVLVIGGGP
                     AGNSAAIYAARKGLKTGILAETFGGQVIETVGIENMIGTLYTEGPKLMAQIEEHTKSY
                     DIDIIKSQLATGIEKKELVEVTLANGAILQAKTAILALGAKWRNINVPGEEEFRNKGV
                     TYCPHCDGPLFEGKDVAVIGGGNSGMEAALDLAGVTKHVTVLEFLPELKADQVLQERA
                     AKTDNLTILKNVATKDIVGEDHVTGLNYTDRDTNEEKHIDLEGVFVQIGLVPSTSWLK
                     DSGIELNERQEIVVDKFGSTNIPGIFAAGDCTDAAYKQIIISMGSGATAAIGAFDYLI
                     RQ"
     gene            complement(12056..12616)
                     /gene="ahpC"
                     /locus_tag="ACUBV3_000800"
     CDS             complement(12056..12616)
                     /gene="ahpC"
                     /locus_tag="ACUBV3_000800"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002273221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HHU3847318.1"
                     /translation="MSLVGKEIIEFSAQAYHDEKFITVTNEDVKGKWAVFCFYPADFS
                     FVCPTELGDLQEQYETLKSLDVEVYSVSTDTHFVHKAWHDDSDVVGTITYPMIGDPSH
                     LISQGFDVLGQDGLAQRGTFIIDPDGVIQMMEINADGIGRDASTLIDKVRAAQYIRQH
                     TGEVCPAKWKEGAETLTPSLDLVGKI"
     gene            12893..13663
                     /gene="rpsB"
                     /locus_tag="ACUBV3_000801"
     CDS             12893..13663
                     /gene="rpsB"
                     /locus_tag="ACUBV3_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HHU3847319.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIHVIDLQ
                     QTVKLADQAYEFVRDAAANDAVILFVGTKKQAAEAVAEEAKRAGQYFINHRWLGGTLT
                     NWGTIQKRIARLKEIKRMEEEGTFELLPKKEVALLNKQRARLEKFLGGIEDMPRIPDV
                     MYVVDPHKEQIAVKEAKKLGIPVVAMVDTNADPDDIDVIIPANDDAIRAVKLITSKLA
                     DAVIEGRQGEDADVDFAQEAQADSIEEIVEVVEGSNND"
     gene            13757..14797
                     /gene="tsf"
                     /locus_tag="ACUBV3_000802"
     CDS             13757..14797
                     /gene="tsf"
                     /locus_tag="ACUBV3_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HHU3847320.1"
                     /translation="MAEITAKLVKELREKSGAGVMDAKKALVETDGDLDKAIELLREK
                     GMAKAAKKADRVAAEGLTGVYVDGNVAAVIEVNAETDFVAKNDQFVTLVNETAKVIAE
                     GRPSNNEEALALTMPSGETLEQAFVTATATIGEKISFRRFALVEKTDEQHFGAYQHNG
                     GRIGVITVVEGGDDALAKQVSMHVAAMKPTVLSYTELDAQFVHDELAQLNHKIEQDNE
                     SRAMVNKPALPFLKYGSKAQLTDEVIAQAEEDIKAELAAEGKPEKIWDKIVPGKMDRF
                     MLDNTKVDQEYTLLAQVYIMDDSKTVEAYLESVNAKAVAFVRFEVGEGIEKASNDFEA
                     EVAATMAAALEK"
     gene            14854..15659
                     /locus_tag="ACUBV3_000803"
     CDS             join(14854..15228,15231..15659)
                     /locus_tag="ACUBV3_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element IS1381A family transposase"
                     /protein_id="HHU3847321.1"
                     /translation="MNYEASKQLTDVRFKRLVGVQRTTFEEMLAVLKTAYQRKHAKGG
                     RTPKLSLEDLLMATLQYMREYRTYEQIAADFGIHESNLIRRSQWVESTLIQSGFTISK
                     THLSAEDTVIVDATEVKINRPKKQLANYSGKKKCHAMKAQAIVTSQGRIVSLDIAVNY
                     CHDMKLFKMSRRNIGQAAKILADSGYQGIMKMYSQAQTPRKSSKLKPLTLEDKTYNHT
                     LSKERIKVENIFAKVKTFKIFSTTYRNRRKRFGLRMNLIAGMINRELGF"
     gene            complement(15817..16278)
                     /locus_tag="ACUBV3_000804"
     CDS             complement(15817..16278)
                     /locus_tag="ACUBV3_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COG2426 family protein"
                     /protein_id="HHU3847322.1"
                     /translation="MNYIITFLISMIPLVELRGAVPFAIANGIPLWEALAIGVVGNML
                     PVPIIFFFARKVLEWGADKPYTGKFFTWCLKKGHSGGQKLEKVAGEKGLFIALLLFVG
                     IPLPGTGAWTGTLAASLLDWEFKHSVIAVMLGVILAGCIMGTLSIIGFNLF"
     gene            16451..16915
                     /locus_tag="ACUBV3_000805"
     CDS             16451..16915
                     /locus_tag="ACUBV3_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HHU3847323.1"
                     /translation="MAIKNTSDNIEEYIKSLLEQSGIAEIKRSNLADTFQVVPSQINY
                     VIKTRFTESRGYVVESKRGGGGYIRIAKVHFSDQHQLFGNMLSTIGERISEQVFDDLI
                     QLLFDEEIITEREGNLILATSGDDVLGEQASVIRARMLRKLLQRLDRKGFHL"
     gene            16912..19359
                     /locus_tag="ACUBV3_000806"
     CDS             16912..19359
                     /locus_tag="ACUBV3_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HHU3847324.1"
                     /translation="MSHYSIKLQEVFRLAQFQAARYESHYLESWHLLLAMVLVHDSVA
                     GLTFAEYESEVAIEEYEAATILALGRAPKEEITNYQFLEQSPALKKILKLAENISIVV
                     GAEDVGTEHVLLAMLVNKDLLATRILELVGFRGQDDGESVRMVDLRKALERHAGFTKD
                     DIKAIYELRNPKKAKSGASFSDMMKPPSTAGDLADFTRDLSQMAVDGEIEPVIGRDKE
                     ISRMVQVLSRKTKNNPVLVGDAGVGKTALAYGLAQRIANGNIPYELRDMRVLELDMMS
                     VVAGTRFRGDFEERMNQIIADIEEDGHIILFIDELHTIMGSGSGIDSTLDAANILKPA
                     LARGTLRTVGATTQEEYQKHIEKDAALSRRFAKVLVEEPNLEDAYEILLGLKPAYEAF
                     HNVTISDEAVMTAVKVAHRYLTSKNLPDSAIDLLDEASATVQMMIKKNAPSLLTEVDQ
                     AILDDDMKSASKALKASYKGKKRKPIAVTEDHIMATLSRLSGIPVEKLTQADSKKYLN
                     LEKELHKRVIGQDDAVTAISRAIRRNQSGIRTGKRPIGSFMFLGPTGVGKTELAKALA
                     EVLFDDESALIRFDMSEYMEKFAASHLNGAPPGYVGYDEGGELTEKVRNKPYSVLLFD
                     EVEKAHPDIFNVLLQVLDDGVLTDSRGRKVDFSNTIIIMTSNLGATALRDDKTVGFGA
                     KDISHDYTAMQKRIMEELKKAYRPEFINRIDEKVVFHSLSQDNMREVVKIMVKPLILA
                     LKDKGMDLKFQPSALKHLAEDGYDIEMGARPLRRTIQTQVEDHLSELLLANQVKEGQV
                     IKIGVSKGKLKFDIAKS"
BASE COUNT         6182 a         3248 c         3810 g         6184 t
ORIGIN      
        1 ggtttgttat gtctaaaaaa atgaccatta atgacatcgc tcaattatca aaaacttcca
       61 aaacaacggt ttcttttttc ttgaatcaaa aatttgaaaa aatgtcggat gagacgaggc
      121 aacgtattca agaggtgatt gatgaaacag gttatagacc aagtacgata gcaagaagtc
      181 taaattctaa aaaaacaaaa ctactaggtg tcttaattgg agatataaca aacacatttt
      241 caaatcaaat tgtaaaagga attgagcaca tcaccaaaca aaaaggctat caaattattg
      301 tgggtaatag taattacgat gccaaaagtg aagaagatta tattgaaaat atgttaaatt
      361 taggtgttga tggtttcatt attcagccta catcaaattt tcgaaagtat tctcgtattt
      421 taaaagaaaa aaagaaacca atggtatttt ttgatagtca actttatgaa cataagacga
      481 gttgggttaa ggctaataac tatgatgctg tttatgatat gacacaggag tgtctaaatc
      541 gagggtataa aaagtttatt atgattactg gtgatacgag cttgcttagt acacgtattg
      601 agagagcttc gggattcatg gatgctttaa aggacaatgg tttcggttat gacactttgg
      661 tgattgaaga tgatgatcat tcaaaaagtg atatagaaga ctttctaaaa gcggtagttc
      721 cagataagga ggagacatta gtatttgcac caaattgttg ggctttacca atggttttta
      781 ctgccatgaa gaatctcaat tttgatatgc cacgagttgg tttagttggg tttgacaata
      841 ttgaatggac agacttttca tctcctaaag tgtcaacaat tgtccagccg gcttatgaag
      901 aaggtgaaca agtagcacag attttgatta atcgtattga aggagatgac tccgtcgata
      961 accaacagat tgtagattgc cagatgtttt ggaaagagtc gactttttaa aaaagatttc
     1021 ttattttatt tgctattaaa aaataaaacc tagagaaaat tatctctagg ttatttgtcc
     1081 ttattctaaa ataagtaagc cttctttgac agcgaaagga tttttctcat tgatacggtc
     1141 gtaaaacata ataccatcaa tgtggtcaat ttcatgttga acaacaatag agttgtaacc
     1201 ttttaatttg agtcgatgtt tttcaccagt tttgtcgaaa tattcaatag ttacacgcgc
     1261 gtggcggacg acatagccag gtacttcacg atctactgat aggcatccct ccccgtcaga
     1321 taaagcagca tcttgaacag aatgcgatac aactttaggg ttgtacatta cttcttggag
     1381 gctataagct tctttaggtg gatttccttg agcatcctca acgtttggaa caaggacggc
     1441 aatgatgcgt tttgagatgt ctaattgtgg tgcggctaaa ccaacaccac cacgtaagcc
     1501 tagcttctca gccataattg gatcttgaga gtgttttaaa aattgcatca ttttttctcc
     1561 gagaatttct tctttctcag aaagtgggaa tgtgacttct tcagccactt tgcgaagggt
     1621 tggattgcct tcacggataa tgtcattcat gtcaattaaa tgactagctt ttactaattt
     1681 atcaatagca gacatatttt ctcctaactg tattctctga tttagtttag cataaaataa
     1741 ttaaatgata aagctacgct tcatggctga atatttaatg attctagtgc gaaacgattt
     1801 ttgataaagt attttggttt ttctttttgg ataaggcctt ctttgaccat atcgtggata
     1861 actcttaata aatgacgata actgacgcca aacgaatcag ctaaactact taactcaagt
     1921 tgaaagtaac cttgttcttc aattgctaaa atatgacttg caagtaactc tttgacggta
     1981 taccctagat tagtagacgc tttgatgttt tgatcatgaa aagcctgagc tagctcttga
     2041 ctaagtttta ggaggaaagt tgcgtctgtt aacagtttct ctttccgtcc tttaagtgat
     2101 aattgaatga ctgtaagatc ctctaaggca ataacagatg atacaatttg tctattagtc
     2161 aaaagttcaa tatcaccaat cagtgttggt tttgttttaa tatctaaaat atgttctttg
     2221 ccgttaaaga gtcgacgtac aattttaaaa cgtcccttga cgatgtagtg caagtattct
     2281 aattgttctc cctgtttaca gacggattgg ccggcagtta gggtgattac ttgtaaatcc
     2341 ttgaaataat gttttggaaa gatagtctcc aaacagtagt cttggcagta gttttctata
     2401 tccatgtatt gactccctat ttgagatagt tttaggacat atgtcatata tttctagttt
     2461 aatattgatt atactttatt tatctaaaaa aagacatcag aaagaggtat tgtcacttga
     2521 aaatctttat ggagaagcta agtttactct ctttatcact tatcttgcta tcaacttttt
     2581 cgacatcacc agccctacca caaatgatat cttattatag agataagggc ttaccatcac
     2641 cccaagttga attattgttt tcaattcctt cgatggctat tatcttcatc ttgcttatca
     2701 caccttggtt aagtaaaaaa ctttctgaga aacatatgat tatctttggg ctattgctaa
     2761 cagcattagg gggaggattg ccggttgtca gtcagaacta ccttttagta tttgtatcgc
     2821 gtcttttatt ggggagtggt attggtttta ttaatgctag ggccattagt gtgattagtg
     2881 aatattatca aggcaaagaa agaagaaaac tcttaggatt aagggggtcc tttgaagttt
     2941 taggaaatgc tggtttgaca gctttagttg ggctgttgtt gacttttggt tggtccaaat
     3001 catttatgat ttatttttta gctttaccaa tcttagttct atatcttgtt tttgcgccta
     3061 agaaagtggt gaaagataca aatgacaaga ttaaaactaa aggtcaaaaa atccctaaag
     3121 ctgatttaac ttatattgtc gccttagcta ttttagcagg atttgtcata actataaata
     3181 ctgggattaa tcttaggata cctctgctag tagtagaatt tggtttgggt acgccagctc
     3241 aagctagtct tgttcttagt gcgatgatgc ttatgggaat aatagcaggt atgagttttg
     3301 gtcagttgat agctatgttt cataaacaat taattccaat ctgcctcgtc ttattttctt
     3361 taacattgct tggagttgga ttgccttcta atctaatggt actaacaatt agtgcgatgg
     3421 catcaggttt cctttacagc ctgatggtca ctgctgtgtt tagcctagta gcagaccgtg
     3481 ttgaatattc gttggttggt tctgcaacga cattagtttt agtattttgt aatattggtg
     3541 gagctagtgc ggctattctt ctgtcttgtt ttgatcattt actaggacaa ataaatgcag
     3601 ttttctatgt ttatgctatt ttaagtttag cagttggtat gatttatttc tttaaacgcc
     3661 tattttttaa gaaaatgtag tgtatattaa ctaaaaaggg cttgacaatc attgtcaagc
     3721 tctcttcatt tttcttcttc tgggtaggta aatccccatt cttgacgtag ctgtgtcatg
     3781 atatccataa cgtctttggt atagtttaag tacatgtggt tagtttttcc tgaaacggct
     3841 tcttccatat cagctacctc atattggagg gcattttcag ttttgccagc ttcgagaata
     3901 tcttgatgcc catcctcagt ataagtaata actgcctttt gtcctcgcgg atattcaaaa
     3961 agttcaatgt agcctttatc gtaagcgata gttgctcgtt taggttgttt tgcatgtaaa
     4021 ctaaggctga cagtcgccat ctcatttgca ggattggtta gtaggatacc aacttgttca
     4081 tcaactcctg ttggtgcaaa tgtaacttga gaggtaatgt tgtgaggtgc ttctgacata
     4141 aaccagcgaa tacaagaaag tgcataaaca ccaatgtcca gcaaagcacc gcctgctagg
     4201 tcacgactga aaaaacggtt agtcatatca tattctttat aacttccgaa attcatttga
     4261 atcattttta acggtcctaa ttttccacta tcaactaatg tttttaattg gcgataaatt
     4321 ggcatatgaa aaatagtcat ggcttcagct aagacaacat ggttagtttc ggctaaatct
     4381 atggcttctt taagttcagt gctgtttaaa gtaatagatt tttcgcaaag aacgtgctta
     4441 ccatttgcta aagcctttcg taaaaatgag atgtgagtat tgtggggagt agagatataa
     4501 atgatatcca cttcagggtc ttcaaatact tgatctatgt gatcataaac tttttggata
     4561 ccatatttgg tagcaaattc aagtcctttg tcgtaagttc tattagctac agaatataat
     4621 ttttgtccac gtgcttctag tgcttgggct aattcgttgg caataacgcc ggttccaagc
     4681 gttgcccaat tataatattt ttcaatcata acaacctcct tgttggttta tagtatagca
     4741 aaaataacaa aatgatattt agagaaagca ccaatatgag agtattttta atagtcaaac
     4801 tctgaatttt ctgttagact gtaagagatt acatatgaga tattaaggag aaaataatga
     4861 taaaatatca agatgatttt taccaagcgg ttaatgggga gtgggctaag acagctgtta
     4921 ttcctgatga caaaccaagg acaggtggtt tttctgatct tgctgatgac attgaagcat
     4981 taatgctgtc aacgacagat aagtggctgg ctgatgaaaa taaaccaagt gatacgattt
     5041 taaatcattt tattgctttt cataaaatga cagctgatta tcaaaaacgt gaagaggtag
     5101 gagtatcacc agtattacca ttaattgaag aatataaagg cctacaatct ttctctgagt
     5161 ttgcatcaaa agttgcagaa tatgaactag agggtaaacc aaatgaattt ccatttggtg
     5221 tggctcctga ctttatgaat gctcaattga atgtactttg ggctgaagcg cctgggatta
     5281 ttttaccaga tacaacttat tatagtgaag ataatgaaaa aggaaaggaa ctccttgctt
     5341 tttggcgtaa aagtcaagaa gacctactgc ctttgtttgg cctttcagaa caggaaatta
     5401 aagatatctt ggataaggtt ttagcattgg atgcaaaatt ggcacaatat gtcttatctc
     5461 gcgaagagag ttcggaatat gttaaacttt accatcctta taactgggaa gatttcacaa
     5521 aattagcacc tgaacttcca ttagatgcta ttttccaaaa gatattagga caaaaaccag
     5581 ataaagttat tgttcctgaa gaacgtttct ggacagaatt tgcatctgac tattactcag
     5641 aatctaattg ggaattatta aaggcagatc ttattttatc agctgcgaat gcctataatg
     5701 cttacttaac tgatgatatt cgtatcaaat cgggagttta cagtcgagct ttatcaggca
     5761 caccacaagc aatggataag aaaaaagcag cttactacct tgcttcagga ccttacaatc
     5821 aagctctagg tttgtggtat gcaggagaaa aattctctcc agaagctaaa gctgatgttg
     5881 agcataagat tgcaacgatg attgatgtct ataagtcacg cttagaaaag gcggattggc
     5941 ttgcacagtc aacgcgagaa aaagctatta tgaaacttaa tgttatcaca ccacatattg
     6001 gataccctga gaaactacca gagacttata ctaaaaaaat cattgatcct aaactatcgc
     6061 ttgtcgaaaa tgctactaat ttagacaaaa tttcaatagc ctatggttgg agtaaatgga
     6121 ataaaccagt tgatagaagt gaatggcata tgcctgccca catggtcaat gcgtattatg
     6181 atccacaaca aaaccaaatt gtcttcccag cagccatttt acaagaacca ttttatgctt
     6241 tagagcaaag ttcatcagca aattatgggg gaattggtgc agtgattgca catgaaatat
     6301 cacacgcttt tgacactaat ggagcttcat ttgatgaaca tggaagtttg aataattggt
     6361 ggactgatga agattttgaa gctttcaaaa aacttacaga taaagtagtt gagcagtttg
     6421 atggactgga atcttatggc gcaaaagtca atggtaaact aacagtatca gaaaatgttg
     6481 cagatttagg tggagttgct tgtgctttag aagctgcgca gcgtgagtca gatttttcag
     6541 cgagggattt ctttattaac tttgcgacaa tttggcgtat gaaggcacgt gatgagtata
     6601 tgcaaatgtt agctagtgta gatgttcacg ccccagcaca atggcgaact aatattacag
     6661 taacgaattt tgaagaattc cataaagagt ttgatgttaa agatggtgat aacatgtggc
     6721 gtccagttga aaaacgtgtt attatttggt aactctaaat gataaactcc cgttactcag
     6781 atgagtagca ggagtatttt tttattcttt tatagataga gataatgtag tagtgtcaac
     6841 gcttagctca gctccaatag tcactgtcaa aagaggttgg gtgtgcgcaa aatccatatc
     6901 ataaataaca ggaatttcct tgaaaatagc atgttttgaa aggatatatt caaagacttg
     6961 tggagtcatg ccgcattctt taggaaagcg ccccataaga atagcttgtg gatgaggata
     7021 agcttgtaaa attgcagcaa ggttgcggtc aaattcataa aaattacttt cctcagcctc
     7081 ttcaatcaag aggacatagc gttcaatttt cggtgcataa ggagtacctc gaagtaggcc
     7141 aaacgtagag agattacctc cgataatcgt accggaagct tttccgtgat tataaatttt
     7201 ccattctgtt gggaaaaatt ggcgtggttt gctgggatca taccagggat cactagacca
     7261 ttcttcacta ggccaaagtt catagtggtt ttctgtcatg gcagtaagcc aagcttgtgt
     7321 ttggtaaggt tgaccttcct tcattttgaa agaagaataa gcagggccca tgtaagtttg
     7381 gatttttgct ttggcaaaaa tagcattaag gaaggccgtt gaatctgagt aaccacaaat
     7441 tattttggga ttcttagaga ttaaatcata atctaaatat ggtaaaagct cgttggaatt
     7501 gaatccgcca atggttgcta atatggcatc aacagaaggg tcggcaaatg cttcatgtaa
     7561 atcttctaca cggctagcaa tcgaagcaga atagaggata tcattttcta gatagtgctt
     7621 agagaatgac acttgaaatc ccagcttttc tagtacttct ttggctaata ggttagcctc
     7681 aaaaccaccc agacgctcaa tggaagaaga gggactaaga acacgaatgt ggctgccttt
     7741 tgttaatttt ttcattggtt aacctcctat ttaaattgtt gttagtctac cacatatgga
     7801 gataatgagc aacgatattc ttaaaatcta tcaatttaag aaatgcgtgt tatactagag
     7861 atagccgatg aaagaaaaaa tacttattat taataatttg gtcattaaat aggaggtcat
     7921 gatgtctcaa ttatctttgg aagaacaatt gaatcgacta aaagataaat acctttcttc
     7981 taaaaatcaa gaattttcaa atgttcactt aagtaaagta aagaagaaaa agaagaaatt
     8041 aataatactt gaaaaagaaa gatgtcgctg tcgattggag agagaagata ctaaaaaaat
     8101 cgatgataaa attgagaaat tgaagaaaga attgatagat gttatgaagg aggaataata
     8161 tggaagaaac tattctaata gtcagctttc tcctgttttt gattttatct aatgttatca
     8221 ataggatatt tcctaaatta ccattaccat ttatccagtt ggtctttggg attctctctg
     8281 gcttagtttt tcataagtca caagtccata tcgatcctga gttattcctt gcctttgtta
     8341 ttgctccttt gaactttagg gaaggtcaag aaagtgatat tggtagtttt ataaagtatc
     8401 gggctattat tctttactta atcttaccaa cggtattttt aacagctatt gtcgttggtt
     8461 atgtagcagg gcaccttttg ccagttagtt taccacttgc ggcatgcttt gcgcttggag
     8521 cagccttagg cccaacagat gccgttgcct ttatatcgat tgctaagagg tttcaatttc
     8581 ctaaacgggc agaaaatatc ctcaaattgg aaggtctttt aaatgacgct agtggtctag
     8641 tttccttcca gtttgcttta acagcccttg taacaggtta tttctcctta gcaaaagcta
     8701 gtctcaagtt agcattggcc ataatgggtg gttttttaat tggcttgctt tttgcttttc
     8761 taatgaggtt atgtctcact gtcctagaga aatttgatgc tgctgatgtt acaggggctc
     8821 tacttttgga attaacctta ccttttgttg cttatttcgt tgctgatttg ttgggatttt
     8881 cagctattat tgcggtggta gtggcaggag taatgcaagc aaatcgtttg aaaaaagtga
     8941 ctctctttga tgctcaagta gatcgcgtta cctctgttat ttgggaaacc ttaaatttta
     9001 ttttaaatgg tttagttttc ttaatatttg gaagagaact gacacgtatc attggtcctc
     9061 ttttaacaag taacgcttat agtaattttg atcttattag tattgttgtt ttagttactt
     9121 gcacactttt tctagttcgc tttctagcag tttcttgctt ttatgcttgg agatctttca
     9181 aataccataa atcatttaaa aagtattgga gagagattca actgttgact ttttcaggag
     9241 ttaaaggaag tgtttccata gcaacaatac tcttattacc caagcactca gtcataggag
     9301 agttggggta tagtttgatt cttttcactg ttggtgcagt aacgctaatg agttttttaa
     9361 cgggactttt agtattacca aagttagcgc ctcccttaca ggtgaaagat gattatttaa
     9421 tacgtctatc gattttaaca aaagtattat ctgtcttaga agaagatggt aagagttctg
     9481 aaaatcaagc ttcattttat gcagttatag ataattataa tagccgtatt cgtcatctca
     9541 ttttagaaca agaatctagt gacattaaga aggacttagc tgaattgcaa ctgatgatgc
     9601 ttagtataga aagcgatgga cttgaagctg cttatcgtta tggcaatatt tctatcaaag
     9661 aatatcgtat ttaccaacgt tatttaaaat acttagaacg ccaagttaat cgtagctttg
     9721 tgtcaaatat cacttatagt ttaacgattc ttatgcgtgt tattcgtggt attgttcatg
     9781 agtttttaac atttggacaa ggaatacgaa atcggaaaaa acatttcaaa aacaggaaac
     9841 ggttgttagc agaagtcaat cgtcagcact taacagattt gtacttatca aataatgacc
     9901 tcattttaga aacgctagaa gatatggaag gttattataa tgcaactttg atagatttct
     9961 tacaaaatga acgcattcga gacgcagaat taattaaatc aggtttattt gtagaacgtg
    10021 tcattgcacg cataacgcca gatattttaa atgaaaccct aagaggatat tacttggaac
    10081 gcagaacaat ctctgaattt gaaagggacg gtttaattag ttctaaagag tctcaaattt
    10141 taagggataa tgtcaatgaa ttagaaagct attcattaag agatagtcaa tcttctatac
    10201 cttatcaatt attggattta aaaaaataat tctgataaca aaaaaatcaa cgaacaatcg
    10261 ttgatttttt gttatctttg gataaaatct tttatattta aggcggtaga cggatttgaa
    10321 ccgacgatca agcttttgca gagccgtgcc ttaccacttg gctataccgc cataacagat
    10381 aatattctat cgaaaaaaaa aagattcgtc aagcctcttt cgtaaaatag ccaattttag
    10441 cccttaaact tcctcatatt acccaaaaaa agagattgat acccattgta tcaatctctt
    10501 ttagattatt gtctgattaa ataatcaaat gcgccaatgg cagctgtggc accagacccc
    10561 atggaaataa tgatttgctt gtaggcagca tctgtacagt cacctgcagc aaaaatacca
    10621 gggatatttg ttgagccaaa tttatcaacg acaatttctt gacgttcatt gagttcaatg
    10681 ccgctatcct tgagccaaga tgtacttggt acaagaccaa tttgtacaaa aacaccttct
    10741 aaatcaatgt gtttttcttc gtttgtatca cgatcggtat agtttaaacc agtgacatga
    10801 tcttcaccaa cgatatcttt agtagcaaca ttcttaagaa tagttaaatt gtcagtttta
    10861 gctgctcgtt cttgcagtac ttggtctgct ttgagctcag gtaagaattc taggactgta
    10921 acatgtttag tgacaccggc tagatctaaa gcagcttcca tacctgaatt accaccacct
    10981 ataacagcaa catcttttcc ttcgaaaaga gggccatcac aatgtggaca gtaagtaaca
    11041 cctttattgc ggaattcttc ctcgccagga acgttgatat ttcgccactt agcacccaga
    11101 gctaggatag cagttttagc ttgtaaaata gctccgttag ccagtgttac ctcaactaat
    11161 tcttttttct caatgccagt tgctaattga ctcttgataa tatcaatatc gtaagatttg
    11221 gtatgctctt caatttgagc cattaattta gggccttctg tgtacagtgt accaatcatg
    11281 ttctcaatac caactgtttc gataacttgt ccaccaaaag tttctgctaa aatacctgtt
    11341 tttaaccctt tacgggcggc ataaattgca gcactgttac ctgcaggacc accaccaata
    11401 actagaacat catagacacc tttgtcagca aatgcttcag catcaagagg tccatctagt
    11461 tgttctaaaa gctgttcaat tgtagcacgg ccagaagtaa attcttcttg gtccttataa
    11521 acagtaggaa cagacatgat accttttgat ttgacttcgt cttgatacat gcctccttca
    11581 atcatagtat gagtgatatt cggattcaaa acagccatta tattaaaggc ttgaacgaca
    11641 tctggacaat tatggcaagt taatgaaaca taagtttcta gattgatagt tttctcaatc
    11701 cccttaattc gtttgataat atcttcatca acttttggtg cgcgaccaga gacttgcaac
    11761 aaagctaaaa tgaaagatgt gaattcatgc cccattggca agcctgaaaa aataacgcga
    11821 ctttcatgcc ctttttttgc aattccaaaa ctaggttgac gttttaaatg tgttgattca
    11881 aggctgatgc gatcagacat agcgacaatt tcatctagaa aatcctttac tttttgagaa
    11941 ttatcgttat ctcctaaatc agcttgtaat acaatatcag attctagcaa atctaaatat
    12001 tgcgctaatt gagccttgat ttctttatct aagaccatgg attacctcct ggttattaaa
    12061 ttttacctac aaggtctaaa ctaggagtta aagtttcagc tccttctttc catttagctg
    12121 gacaaacttc acctgtatgt tggcggatgt attgagcagc acggacttta tcaatgagag
    12181 tacttgcatc acgtccgatg ccatcagcat taatttccat catttggatg acaccgtctg
    12241 gatcaatgat aaatgtacca cgttgtgcta gaccatcttg tccaaggaca tcaaaacctt
    12301 gtgaaataag gtgtgaaggg tcaccaatca taggatatgt gatagtacca acgacatctg
    12361 agtcatcatg ccatgcttta tgaacaaaat gagtatcagt agagacagaa taaacttcta
    12421 catcaagaga tttaagtgtt tcgtattgct cttgaaggtc accaagttca gttgggcaaa
    12481 caaatgaaaa gtctgctggg tagaaacaaa aaactgccca ttttccttta acgtcttcat
    12541 ttgtaacagt gatgaatttt tcatcgtgat aagcttgagc tgaaaattca ataatttctt
    12601 ttccgactaa tgacataata atgtcctcct ttatttttta atagcctaat tataaacgaa
    12661 atacttttaa atctccaata aaaacgttta catttttcta agaaaagtat gatttttcta
    12721 atagagtaga agattttata gcttgtcaaa tctaactttt tttgataaac tattatagtt
    12781 gcttaaagtg acaaatactc atcttagatc aattgtggtc ttgttgccga aaggttaggc
    12841 tgcaagtcga agtctgagag aggaaaaaac atttttaaag gagaacttac tcatggcagt
    12901 aatttcaatg aaacaacttc ttgaggctgg tgttcacttt ggtcaccaaa ctcgtcgctg
    12961 gaaccctaag atggctaagt acatcttcac agaacgtaat ggtatccacg ttatcgacct
    13021 tcaacaaact gtaaaattag ctgaccaagc ttacgaattt gttcgtgatg ctgcagcaaa
    13081 cgatgcagtt atcttgtttg ttggtactaa gaaacaagca gctgaagctg ttgctgaaga
    13141 agctaaacgt gctggtcaat acttcattaa ccaccgttgg ttaggtggaa ctcttactaa
    13201 ctggggaact atccaaaaac gtattgctcg tttgaaagaa atcaaacgta tggaagaaga
    13261 aggaactttt gaacttcttc ctaagaaaga agttgcattg ttaaacaaac aacgtgcacg
    13321 tcttgaaaaa ttcttgggtg gtatcgaaga tatgccacgc atcccagatg taatgtatgt
    13381 tgttgaccca cataaagagc aaatcgctgt taaagaagct aaaaaacttg gtattccagt
    13441 tgtagctatg gttgacacta acgctgatcc agatgatatc gatgttatca tcccagcaaa
    13501 cgatgacgct attcgcgccg ttaaattaat tacttctaaa ttagctgatg cagttattga
    13561 aggtcgtcaa ggtgaagatg ctgacgttga cttcgctcaa gaagcacaag cagattcaat
    13621 tgaagaaatc gttgaagtag ttgaaggatc aaacaacgac taatatttaa tttattgtaa
    13681 atcctaaaac ggggcagatg caaaccgctc tgtctcgttt ttttaatcat tatttgaaat
    13741 taagaggaga acgaaaatgg cagaaatcac agctaaactt gtaaaagaat tgcgtgaaaa
    13801 atcaggtgca ggcgttatgg acgctaaaaa agcattagta gaaactgatg gtgaccttga
    13861 taaagcgatt gaattacttc gcgaaaaagg tatggctaaa gcagctaaaa aagcagaccg
    13921 tgttgctgct gaaggtttaa caggtgttta tgttgatggt aacgttgcag cagttattga
    13981 agttaacgct gagactgact ttgttgctaa aaacgaccaa tttgtaacat tagttaatga
    14041 aactgctaaa gttattgcag aaggacgtcc ttcaaacaat gaagaagcac ttgctttaac
    14101 aatgccttca ggtgaaactt tagaacaagc atttgtaact gcaactgcta caattggtga
    14161 aaaaatttca ttccgtcgct tcgctttagt tgaaaagact gatgagcaac attttggtgc
    14221 ataccaacat aatggtggac gtatcggagt aattactgtt gttgaaggtg gcgacgatgc
    14281 acttgctaaa caagtatcaa tgcacgttgc agctatgaag ccaactgttc tttcatatac
    14341 tgaacttgat gcacaatttg ttcatgatga attagcacag ttgaaccaca aaattgagca
    14401 agacaacgag agtcgtgcta tggttaacaa accagctctt ccattcctta aatatggatc
    14461 aaaagctcaa ttgactgatg aagttattgc ccaagcagaa gaagacatca aagcagaatt
    14521 agctgcagaa ggtaaaccag aaaaaatctg ggataaaatc gttccaggta aaatggatcg
    14581 tttcatgctt gacaacacta aagttgacca agagtacaca ctccttgctc aagtttacat
    14641 catggatgat agcaaaacag ttgaagctta tcttgagtca gtaaatgcta aagcagttgc
    14701 tttcgtacgt tttgaagttg gagaaggtat tgagaaagct tctaacgact ttgaagcaga
    14761 agttgcagct acaatggcag cagcacttga aaaataatta acactcgctt tatattagac
    14821 ttcctgcgaa acaaaatatg gtacaatagt tctatgaatt atgaagcaag caaacaatta
    14881 actgatgtac gatttaagcg ccttgttggt gttcagcgca ctacttttga agagatgtta
    14941 gctgtgttaa aaacagctta tcaacgtaaa cacgcaaaag gtggccgaac ccctaagtta
    15001 agcttagaag atctcctcat ggctactctt caatacatgc gagaataccg cacttatgaa
    15061 caaattgcgg ctgattttgg cattcacgaa agcaacttaa tccgtcgaag tcaatgggtt
    15121 gaatcaactc ttattcaaag tggttttacg atttcaaaaa ctcatcttag tgctgaggat
    15181 acggtgattg tggatgcaac agaggtaaaa atcaatcgtc ctaaaaaaat caactagcga
    15241 attattctgg taaaaagaaa tgtcatgcta tgaaggctca ggcgattgtc acaagccaag
    15301 ggagaattgt ttctttggat attgcagtga actattgcca cgatatgaag ttgttcaaaa
    15361 tgagtcgcag aaacatcgga caagctgcta aaatcttggc agacagtggt tatcaaggga
    15421 tcatgaagat gtattcacaa gcgcaaactc cgaggaaatc aagcaaactt aagccactaa
    15481 ctcttgaaga taaaacctat aaccatacgc tatccaaaga gagaatcaag gttgagaata
    15541 tttttgccaa agtaaaaacg tttaaaatat tttcaacaac ctatcgaaat cgacgcaaac
    15601 ggtttggatt acgaatgaat ttgattgctg gaatgatcaa ccgtgaacta ggattttagt
    15661 ttcgcaggaa gtctattata atttgacagc aaaatgttgt cagtaaatat attttaaaat
    15721 aagacacttg gcttaagtca agtgtcttat tttgctatcg aattggtgga ttggcaaata
    15781 ttaaaaagga gagacagtca tctcacctta acttttctag aataggttga agccgataat
    15841 ggataatgtt cccatgatgc atccagctag tataacacct agcataacag caataacact
    15901 atgtttaaat tcccaatcta ggaggctagc tgcaagcgtg ccagtccagg ctcctgttcc
    15961 gggtaaggga atgcctacaa acaatagcaa ggcaataaac aatccttttt ctccagctac
    16021 tttttcaagt ttttgaccac cgctatgtcc ttttttaagg caccatgtga aaaacttacc
    16081 agtataaggt ttatctgctc cccattctag aactttgcga gcaaagaaga agatgatagg
    16141 tacaggtagc atgtttccaa caacaccgat agctagcgct tcccacaaag gtattccatt
    16201 agcgattgca aatggcacag ctcctcgtag ttccactaag ggaatcattg aaatcaaaaa
    16261 tgtaataatg taattcatta aataaaacct ttctatggta ttattccatt ttatcttatc
    16321 tttctagtaa tgacaactaa aatccgcggt cattctagtt ttactgtcct tagatagatt
    16381 tcatttccgt atcttttaaa aatttgatat aataatagtc agttatagtc aaagaggaag
    16441 gagtgaattt gtggccataa aaaatacttc cgataatatt gaagaatata taaaatcatt
    16501 attagaacaa tcaggaattg ctgaaataaa gcgttccaat ttagctgata cttttcaagt
    16561 tgttcctagt cagattaatt atgtcattaa gacacggttt actgaaagcc gtggttatgt
    16621 ggttgagagt aaacgaggtg gtggaggtta catccgcatt gctaaggttc atttttctga
    16681 tcaacaccaa ttatttggaa atatgttatc aacgattggc gagcgtatca gtgaacaagt
    16741 ttttgatgat ttgattcaat tattattcga tgaagagata attacagaac gtgaaggaaa
    16801 tttgattctg gcgacatcag gtgatgacgt cttaggagag caagcctctg ttattagagc
    16861 tcgtatgctc cgtaaattat tacaacgatt agatagaaaa ggattccatt tatgagtcat
    16921 tattccataa aattacaaga agtattcaga ttggcgcaat ttcaagcagc acgctatgaa
    16981 agtcactatt tagaaagctg gcatcttctt ttagcaatgg ttttagttca tgattcagta
    17041 gctggtttga catttgctga atatgagtct gaagttgcaa ttgaagagta cgaagcagca
    17101 acaattttag ctttaggtag agcaccgaaa gaagagatta ccaattatca atttttagag
    17161 caatcaccag ctttgaaaaa gatcttaaag ttagcagaaa atattagtat cgtagtagga
    17221 gctgaagatg tgggtactga acatgtcttg cttgctatgt tggttaataa agatttatta
    17281 gccacacgta tcttagagtt agttggcttt agaggtcaag acgatggtga atcagttcgt
    17341 atggttgatt tacgtaaggc tctagaacgt catgccggtt ttacaaaaga tgatatcaaa
    17401 gcaatttatg aattacgtaa tcctaaaaaa gcaaagtcgg gtgcttcttt ttctgatatg
    17461 atgaaaccac cgagtactgc tggtgattta gctgatttca cacgtgattt atcacagatg
    17521 gcggttgatg gggaaattga acctgttatt gggcgtgata aagaaatttc acgtatggtc
    17581 caagtcttaa gtcgtaaaac aaagaataat cctgtcttag ttggagatgc gggtgtcggt
    17641 aaaacagctt tggcgtacgg tctagcacaa cgtattgcta atggaaatat tccttatgag
    17701 cttagagata tgcgtgttct agagttagat atgatgagcg ttgttgctgg aactcgtttt
    17761 cgtggtgatt ttgaggaacg tatgaatcaa atcattgcag atattgaaga agacggtcat
    17821 attattcttt tcattgatga actgcatact attatgggtt ctggctctgg tattgatagc
    17881 acacttgatg ctgctaatat tttaaaacca gctttggccc gaggcacttt acgcactgtt
    17941 ggagcaacaa ctcaagagga atatcaaaag catattgaaa aagatgctgc tttatctcgt
    18001 cgctttgcaa aagttttagt agaagaacca aatcttgaag acgcatatga aattctactt
    18061 ggattgaagc cagcgtatga agcctttcat aatgttacta tttcagatga agcagttatg
    18121 actgctgtaa aagtggctca tcgttatttg acaagtaaga atttaccaga ctcagctata
    18181 gacttacttg atgaagcttc tgccactgtg caaatgatga ttaagaaaaa tgctccttcg
    18241 cttttaacag aagttgacca agctatttta gatgatgata tgaaatctgc ttctaaagct
    18301 ctaaaagcaa gttataaggg taaaaagcgt aagcctattg ccgtaacaga agatcatatt
    18361 atggcaactc tcagtcgtct atctggtatt cctgttgaaa aattgacaca agctgatagt
    18421 aaaaaatacc tcaatttaga aaaagaattg cataaacgtg ttatcggtca agatgatgca
    18481 gttactgcca ttagtcgtgc tatccgtcgc aatcaatctg gcattcgtac tggtaagcgt
    18541 cctattggtt cctttatgtt cttgggacca actggtgttg gtaagacaga gttagcaaaa
    18601 gccttggcag aagtgttgtt tgatgatgaa tcagccctaa ttcgttttga tatgtcggaa
    18661 tatatggaaa aattcgcagc ttctcacctg aatggggcac ctccaggtta tgttggttat
    18721 gatgaaggag gagagttgac tgaaaaagta cgcaataaac catattcagt tttacttttt
    18781 gatgaagttg aaaaagctca tccagatatt tttaatgtat tattacaagt gcttgatgat
    18841 ggcgttttaa cagatagtcg aggacgaaaa gttgattttt caaatactat aatcattatg
    18901 acttctaatt taggagcaac tgcattacgt gacgataaaa cagttggttt tggagctaag
    18961 gatatcagcc atgattatac tgctatgcaa aaacgtatta tggaggagct taaaaaagct
    19021 tatcgaccag agtttatcaa tcgtattgat gagaaagttg tattccatag tctcagtcaa
    19081 gataatatgc gtgaagttgt gaaaattatg gttaaacctt taattttagc gcttaaagat
    19141 aaaggtatgg atctgaaatt ccagccttca gccttgaaac acttggcaga ggatggctat
    19201 gatatagaaa tgggtgctcg tccactgcgt agaactattc aaacgcaagt agaggatcat
    19261 ttatcagaac tactattggc aaatcaagtg aaagaggggc aagtaattaa gattggcgta
    19321 tccaaaggca aattgaaatt tgatattgca aaatcataaa acattccagt accgatgggt
    19381 actggaattt taatataaaa agaaaacgtc caaaatattt tgga
//
LOCUS       DBJYSH010000029        19276 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.029, whole genome shotgun sequence.
ACCESSION   DBJYSH010000029 DBJYSH010000000
VERSION     DBJYSH010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 19276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19276
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.029"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            complement(39..818)
                     /locus_tag="ACUBV3_000807"
     CDS             complement(39..818)
                     /locus_tag="ACUBV3_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HHU3847325.1"
                     /translation="MSKVKVLSLITVSGLFLMAGNLSASADVVTSGGDTIMLSGVDAG
                     VSDSITPPPSNINPVTDTIEPSAPTPSTDPITDTIEPSAPTPSTDPITDTTAPSAPTP
                     STDQTTGTTDSSTSAPSTDQTTDTTDSSTSAPSTEAEKPAPSKEPDAPKPITKPIVDV
                     PISTVTGDTVIGTENGKVIVQSASGTILKDAKEVGGEVQKDGTVAIKKSDGKIEVLPN
                     TGEGKTIFTIVGLLLIAGAVWIGFKDNIKKYLTFFKKKSEK"
     gene            complement(835..1383)
                     /locus_tag="ACUBV3_000808"
     CDS             complement(835..1383)
                     /locus_tag="ACUBV3_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3847326.1"
                     /translation="MKRRKIVLFLMVLVMAFIVYKVIQKDNPVIKTSGNHTKVERVEP
                     KHKVKSYDSTKETKSTLEASDRQVSDKEKEKVKRSITVPIDYLVKIPTQSDIKGDYQN
                     KLSITNPTVAQTVKTMLMAGYHFDLESLQVYESDASNVYQFTINLVTDKRDKLSLAGN
                     YVLGTDQFEIASVHGTPVNVVF"
     gene            complement(1383..2756)
                     /locus_tag="ACUBV3_000809"
     CDS             complement(1383..2756)
                     /locus_tag="ACUBV3_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006740735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tip lysozyme"
                     /protein_id="HHU3847327.1"
                     /translation="MGILIVFPFLLVLLLTASSSSQQDCQITPDVSSQVETSSENASN
                     SDWTKKGTVANQTAEKVFNAWVSKGLSGASASGIVGWVNSEGGFAMIGRAEGHYGNDL
                     KTNSIAFNVRPTGLSYYTTEAGGGIYQITPFTKYAPLGDPKWEDADAMNAFVMKSIKS
                     GDWNKAMDLSGGNHSFEEMAQMTDPKQATLVWNAYERGNTAYINKAQKTADGQKAYEI
                     FNGSKYKFDAEKFSKSFGGGSKTSSSGKSDIISDSTVLSLCDSASNAVQGLFGKDKSG
                     KVKYTSYNAWKPDQLPSDLKKYAIDPKSVGLFYRTSSGWNAIASTGGQCTDLSASLMY
                     GLWLKGGMHPTQRMGNGNMVVSNWVSAFGGRSENTPSSGAVFSSSGTSSAGHTGVVSH
                     VFENGDMLIVEQNYSTYSGDNRGFGPYSWNYRYVSTLELKKEHYSFYNPSKAGYKLSD
                     KVKTVGS"
     gene            2988..3062
                     /locus_tag="ACUBV3_000810"
     CDS             2988..3062
                     /locus_tag="ACUBV3_000810"
                     /inference="COORDINATES: protein motif:HMM:NF028245.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3847328.1"
                     /translation="MFIAGNFVIALVKLVLELVKSTKK"
     gene            complement(3252..5252)
                     /locus_tag="ACUBV3_000811"
     CDS             complement(3252..5252)
                     /locus_tag="ACUBV3_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3847329.1"
                     /translation="MDKIDDGLIASTFAKVKDVDIFALKAYMEITHGAETGAQSILLD
                     VFVNFPFFLLNLIVGLFSVILRFFENFSLYDTYKQTVYHSSQKLWENLSGNGSYTSSL
                     LYLLVAISAFSIFISYLFSKGDFSKRLIHLFVVIILGMGYFGTIQSTSGGIYILDTVH
                     QLAGSFSDAVTNLSLDNPSGGKTKITQKSSVADNYVMKTSYTAYLFVNTGQLNGKFHN
                     NQTGKEEKFDNEQVLGKYDKSGKFITPKQKDILNYTDNLGDKATEGEEKNRWLSAVND
                     YLWIKSGYVILKIFEAVILAVPLILIQLIAFMADVLVIILMFIFPLALLVSFLPRMQD
                     IIFNVLKVMFGAVSFPALAGFLTLIIFYTQTLIATFVKKKFTDGSLLSGSNFKGQAIL
                     FMLLITVFVQGCVFWGIWKYKETFLRLIIGSRASQVINQSADRINEKANDLGITPKSI
                     YERAHDMSSLAMMGAGYGVGTMMNAQDNWNAFKERQQANLDDGQSKTNDADKYDEANA
                     DDTVISKEAELTNEGEYQSELPKEASKRIEQLGKESSYELSFISEGNSTEEILKNVKS
                     DNHTFQEGDGDTSLTNQDMITNDIENHSNNYTSPLKQRKLNKLEGELSQFNSDVSMTK
                     NHGKNAFEKGFNASKTKEVRKQHNLERQSKVLEELEKLREGK"
     gene            complement(5268..7769)
                     /locus_tag="ACUBV3_000812"
     CDS             complement(5268..7769)
                     /locus_tag="ACUBV3_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HHU3847330.1"
                     /translation="MVKKLEYPIDRIHQNLALRKDKVVVAYYRIPNTPITITDSDKKD
                     SHKAKVAQVIKKLAKYKNFDISLIPKDYLLGEKMSDFEADLADDVKELGLETLDFTVD
                     TLTHEMEIPYQFDWLIGVDLGKGQYNANIKEFIYNQFESIASNFASLAGYEVEVDEDW
                     YKEHSEEELLVYSLLSTLKAKRLTDVDLFYYQRMQFLRYVPHTKSEVIANRNMLNVTD
                     TLIKSLEGGFLKLESAYGSSFVSVLPVGRFSTIFNGFHLGELVQRMSFPVELRFQAEF
                     IDKTKLGGTMGRSNTRYDQIMKEAYNTNTVQQDDILMGAYSLKDLMKKVGNKEEIIEY
                     GCYLVVAGSSLNQLKQRRYAILSYFDDMKVNVYEASHDTPYLFQALLYGQDLQKTTRK
                     WNHLVTARGFSELMLFTNTQSGNRIGWYIGRVDNRLTAWDSIDEAIMGSKNLVLFNAT
                     VANKEDVAGKVTKNPHVIITGATGQGKSYLAQMIFLHTAQQNVRVLYIDPKRELRQHY
                     LKVVSDPEYARKFPLRKKQIEETNFVTLDSSVKENHGVLDPIVILDKEGASSTAKNML
                     LYLLKNATEIKLDQTTALTEAISQVIAKREAGEVVGFNQVIEALIDSESDEVQSVGRY
                     FKAIIQNSILELAFSDGDVAGLSYEERVTVLEVADLSLPKDGSDHISDHESNSIALMF
                     ALGAFCKHFGERSDDETVEIFDEAWVLMQSSEGKAVIKSMRRVGRSKYNVLMLVSQSV
                     HDAENDDDTTGFGTIFSFYEKSEREDILKHVGLEVTPKNLEWIDNMISGQCLYYDVYG
                     NLNMISIHNIHPDIDPLLKPMKKTVSSHLENKYAS"
     gene            complement(7790..8200)
                     /locus_tag="ACUBV3_000813"
     CDS             complement(7790..8200)
                     /locus_tag="ACUBV3_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HHU3847331.1"
                     /translation="MEEEKYFDYSRGLNAPYWIQEVKTAKGKVIWYFSTPMQLSFFIV
                     FFIILVLMLTVCSPLMQVLNALTHSVSMLLYWFVPSKLAKFYVEYEPQGKKMHIFLWD
                     YLVYLKDFGLNKKGIYQGERVDIVEEIVFEKTKI"
     gene            complement(8204..8470)
                     /locus_tag="ACUBV3_000814"
     CDS             complement(8204..8470)
                     /locus_tag="ACUBV3_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3847332.1"
                     /translation="MNLVKVNSVILAGGPNVSGLKQFFQGDGIYLISVIAVFIIIKNW
                     KEANWLKVFSTLAIYAIIVSMLKGQQILSFLGGMLRWFGVETGL"
     gene            complement(8491..9444)
                     /locus_tag="ACUBV3_000815"
     CDS             complement(8491..9444)
                     /locus_tag="ACUBV3_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HHU3847333.1"
                     /translation="MIDIQKIKKFLLRYTKKEEGGKQPKTKEVKQRTANFIVYGILGL
                     LFIVGFFGSLRAIGLSNQVQHLKETVIAVEKKSKHKKTDDSLDISRIQYYMNNFVYYY
                     INYSQDTADQRKTELENYYSFSTASMTDDVRKSRTLQTQRLISVEKEKDYYIALMRIG
                     YEVDKKSYQMNLAVPFQMQRGLLAIVSQPYTVAEDLYLGKSKAFEKKTLDQVKELSKE
                     QVSSIQKFLPVFFNKYALSNKTDLKLLMKTPELMGKGFKVSELDLNNAIYYQEKKHQV
                     VQLSVTFEDLVTGGTRSENFTLYLSKADNGWYVEEMYHYFK"
     gene            complement(9497..9751)
                     /locus_tag="ACUBV3_000816"
     CDS             complement(9497..9751)
                     /locus_tag="ACUBV3_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002279547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HHU3847334.1"
                     /translation="MFNFTEEAWKDYVSWQQEDKKILKRINRLIEDIKRDPFEGIGKP
                     EPLKYHYSGAWSRRITEEHRLIYMIEDGEIYFLSFRDHYK"
     gene            complement(9744..10010)
                     /locus_tag="ACUBV3_000817"
     CDS             complement(9744..10010)
                     /locus_tag="ACUBV3_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HHU3847335.1"
                     /translation="MEAIVYSHFRNNLKDYMKKVNDEFEPLIVVNKNPDENIVVLSQD
                     SWESLQETIRLMENDYLSHKVINGISQVKEKQVTKHGLIEVEDV"
     gene            complement(10155..10628)
                     /locus_tag="ACUBV3_000818"
     CDS             complement(10155..10628)
                     /locus_tag="ACUBV3_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3847336.1"
                     /translation="MIAIIRTREKGRSQFLCELDIMQFTENQVRNRMIEKGIKDDAFF
                     ICGFSDWNVDRIMSLTEVYLLKRCIEGLYDGDDYIVQYLLKKGLSVHSIVTKYYIFLS
                     NNENKVMKYILRKVEFDSLIDFWQRSVTWVNALNAYIEEGIVLNTSKGFYVLKTE"
     gene            complement(10636..11118)
                     /locus_tag="ACUBV3_000819"
     CDS             complement(10636..11118)
                     /locus_tag="ACUBV3_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000383283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HHU3847337.1"
                     /translation="MDVSAIVRDIKTGRATLVCFPVEMSELKLALGLREEDDLEYIIA
                     DSDCQLLKEHDSIEMINQFVELVENVDSELVKAVHQVIGYTASDFVDYDFNFGDCCLL
                     SDVTTRRELGEYYFDELGVQGVGKEALEMYFDHEAYGRDIDLESQGGFSDYGYVEISG
                     "
     gene            complement(11171..11443)
                     /locus_tag="ACUBV3_000820"
     CDS             complement(11171..11443)
                     /locus_tag="ACUBV3_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3847338.1"
                     /translation="MSNTRSEADKKLLVVTQELSELLVSHQYEQSWEKAGELNSLLKK
                     REELTLPGYMVDMIQQHLKSYYYQNNMINKAHKSMSAIGHKLQEFH"
     gene            complement(11575..11928)
                     /locus_tag="ACUBV3_000821"
     CDS             complement(11575..11928)
                     /locus_tag="ACUBV3_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HHU3847339.1"
                     /translation="MIENFALNLIRLRKQKGLSQKELAKKIGMLPQTISNIENQKGYP
                     TFSNLDKIARYFNVSATELFGTPQQIELERVMFQTDEYSRKAEKILSAVSVFENFVKK
                     YDYQKIIEFGLSLKE"
     gene            complement(11921..12151)
                     /locus_tag="ACUBV3_000822"
     CDS             complement(11921..12151)
                     /locus_tag="ACUBV3_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HHU3847340.1"
                     /translation="MNVTDFKSNFIFPKRLKSLRQESGLKQQEIAPLLGIKQNTYSDW
                     ETGKSEPNLENVVKLAKLFKTTTDFLLGVTDD"
     gene            complement(12138..12515)
                     /locus_tag="ACUBV3_000823"
     CDS             complement(12138..12515)
                     /locus_tag="ACUBV3_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000287184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3847341.1"
                     /translation="MYSNREFAYFVNRRNNLDKMIDLLVFMIPDREFYYPEIQTGELR
                     DYQIDIYDLIKIGYVGVYEIQKDYEDKLRELADFKRKLLKFGLLMQPLDRQKEIVMNL
                     ASQYRLHKRLLKLRENFRGDERD"
     gene            complement(12531..13763)
                     /locus_tag="ACUBV3_000824"
     CDS             complement(12531..13763)
                     /locus_tag="ACUBV3_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HHU3847342.1"
                     /translation="MDAIYLKKFRKKTGLKQKEFALSSGLTLKSLRNYEQGKRKLTLE
                     KYQEIKSHFGYLVENDSSRLQVMIDYVRITLKDVRDLEFFCRNFLHCAFKEFQPFESK
                     LMNYNHLWKRGDIWIFDFADKHETGNFQITVQLSGRGCRQLELLMETEKFTWHDWLSY
                     LRNSYRDDMNVTRFDIAIDELYLGKDRENEQFHLSDMISKYYRHELDFESLRTWNYIG
                     GGSLNFSDMEEIEQNRQGISLYFGSRQSEMYFNFYEKRYEIAKQEGITVEEALEIFEL
                     WNRYEIRLSQSKANAAVDEFISGVPIGEISRGLIVSKIDVYDGKNEYGSFQADRKWQL
                     MFGGVEPLKFVTKPEAYSIERTLRWLSDSVSPSLAMIREYDMIVDGDYLQTILNSGEV
                     NERGEKILDSIKASLGIL"
     gene            complement(14051..15769)
                     /locus_tag="ACUBV3_000825"
     CDS             complement(14051..15769)
                     /locus_tag="ACUBV3_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HHU3847343.1"
                     /translation="MARLIPQYRGIKVKPYMRYMSYYLFSFLFILFLTPVGVYSYYYL
                     DLLKMMDKMSMISVGTGLFLAFFVSWYLTWFLQEANPLFNKLDRLKRMSKFLYENGYV
                     YEKRKKSNKKTKTKYRFPKVYVKQGKYDLSVSFEMAGGKFQKKFKDIGGELEDTFFMD
                     FMEKTDDPRFKIYKLAYSAFLSRITVKDVIWDKDKGIKLMDGYYWDFINDPHLLVAGG
                     TGGGKTVLLRSILRCLAEIGVCDICDPKRADFVTMSDLSAFEGRIAFEKADIIEKFEN
                     AVTIMFARYDFVRNEMKRLGHKDMKKFYDYGLEPYFFVCDEYNALMSSLSYQEREIVD
                     NAFTQYILLGRQVGCNAIIAMQKPSADDLPTKIRSNMMHHISVGRLDDGGYVMMFGDE
                     NRNKEFRFIKYLAGRRVYGRGYSAVFGEVAREFYSPLLPKNFSFYDAFEKINRHENPF
                     DPTENQEVSKAILKDESLREFVEKTSENELLKGSVGFDFDDEMEEMENMSIGDLAKKT
                     GFSFGKIKNLIDMIEKDNYMTFSREDEKVVLNDSDAVVLESLLTQKDEFEGSWKELLL
                     NYFKGE"
     gene            complement(15789..16250)
                     /locus_tag="ACUBV3_000826"
     CDS             complement(15789..16250)
                     /locus_tag="ACUBV3_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001062569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3847344.1"
                     /translation="MAKIGLNFGEKLQIADKEYRTFNDGLDVNVLLGDLTWRRYEEPV
                     QFTEDDTTQPPDRFGRYPQKPTGEVKWNDIAVFSTAQNETIFVSITDMPSYAIEDLGL
                     KLGDPIELSDVVVTWSNVSGGMFKIFASSLKKKDVANNGNASKNEASKVNK"
     gene            complement(16272..16568)
                     /locus_tag="ACUBV3_000827"
     CDS             complement(16272..16568)
                     /locus_tag="ACUBV3_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3847345.1"
                     /translation="MKQETLVLLPNFIGDAIYEGFEAGPAQLNGKKGSYTRHILFNEK
                     HGKFYVYTPASSRVIGNGKMVTVEKPLLFIDNTINGRNIEPAKNVWAEKLIEVK"
     gene            complement(16628..19219)
                     /locus_tag="ACUBV3_000828"
     CDS             complement(16628..19219)
                     /locus_tag="ACUBV3_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0018262 - isopeptide cross-linking
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SspB-related isopeptide-forming adhesin"
                     /protein_id="HHU3847346.1"
                     /translation="MYKNQNTKGHGFFRKSKAYGLVCGIALAGAFTLATSQVSADQVT
                     TQATTQTVTQNQAETVTSTQLDKAVATAKKAAVAVTTTAAVNHATTTDAQADLANQTQ
                     TVKDVTAKAQANTQAIKDATAENAKIDAENKAESQRVSQLNAQTKAKIDAENKDAQAK
                     ADATNAQLQKDYQAKLAKIKSVEAYNAGVRQRNKDAQAKADATNAQLQKDYQAKLALY
                     NQALKAKAEADKQSINNVAFDIKAQAKGVDNAEYGNSIMTAKTKPDGSFEFNHDMIDG
                     VKTIGYGKLTGKVNHHYVANKDGSVTAFVDSITLYKYEYRNVAQNAAVNQNIAFRVLT
                     KDGQIIFEKKHNGNKTFAETLNKTLQLNLKYELKPHGSSGSVEVFKIHDDWVHDTHGS
                     ALVSYVNNNDVVPNVVIPKQPTPPSTEKVTPEAEKPVPEKPVEPKFVTPTLKTYTPAQ
                     PKVKPHVSIPEKINYSVSVHPVLVPAANPSKAVIDEAGQSVNGKTVLPNAELNYVAKQ
                     DFSQYKGMTASQGKIAKNFVFIDDYKDDALDGKSMKVNSIKASDGTDVSQLLEMRHVL
                     STDTLDEKLQTLIKEAGISPVGEFYMWTAKDPQAFYKAYVQKGLDVTYNLSFKVKKEF
                     TKGQIQNGVAQIDFGNGYTGNIVVNDLTTPEIHKDVLDKEDGKSINNGTVKLGDEVTY
                     KLEGWVVPTGRSYDLFEYKFVDQLQRTHDLYLRDKVVAKVDVTLKDGTVIKKGTNLGE
                     YTETVYNKKTGLYELVFKKDFLEKVARSSEFGADDFVVVKRIKAGDVYNTADFFINGN
                     KVKTETVVTHTPEKPKPVEPQKATPKAPAKGLPQTGEASVAPLTALGAIILSAIGLAG
                     FKKRKEN"
BASE COUNT         6125 a         3880 c         2523 g         6748 t
ORIGIN      
        1 aacaaaacta aagccatgaa attaatcatg gcattatctt atttttcact tttctttttg
       61 aaaaaggtta aatacttttt tatattatct ttaaaaccaa tccaaactgc tccagcaatt
      121 aacaagagac ctactattgt aaagatagtc ttaccctcac cagtgtttgg caatacttct
      181 atttttccat ctgatttttt aattgctaca gtaccgtctt tttgtacctc accaccaacc
      241 tctttggcat cctttagtat tgttccagat gcactttgaa caatcacttt tccattttca
      301 gtgccaatta ctgtatctcc tgtaactgtt gaaattggaa catcaacaat aggttttgta
      361 ataggcttag gcgcatctgg ttctttacta ggtgctggtt tttctgcttc tgtacttggt
      421 gctgacgtac ttgaatctgt cgtatctgtt gtttgatctg tacttggtgc tgacgtactt
      481 gaatctgtcg tacctgttgt ttgatctgta cttggtgttg gtgcacttgg tgctgtggta
      541 tctgttattg gatctgtact tggtgttggc gcacttggtt ctatagtatc tgttattgga
      601 tctgtacttg gtgttggcgc acttggttct atagtatctg ttactggatt tatatttgat
      661 ggtggtggcg ttatagaatc agatacacct gcatctaccc ctgatagcat tatagtatca
      721 cctccactag ttacaacatc tgcacttgct gataaattac cagccattaa aaatagtcct
      781 gaaacagtaa ttaaacttaa aacttttact tttgacataa attttccttc tttcttaaaa
      841 tactacattt acaggtgtac catgtacact cgcaatctca aattggtctg tacctaatac
      901 ataattacca gctaaactta atttgtctct tttatctgtt actaaattaa tagtaaactg
      961 ataaacatta ctcgcatctg actcatatac ttgtaaactt tccaaatcaa aatgataacc
     1021 agccatcaac atggttttta cagtttgggc aactgtagga tttgtaatgg ataatttatt
     1081 ttgataatca cccttaatat ctgattgtgt tgggattttt acaaggtaat caataggtac
     1141 agttatagat cttttaacct tctccttttc cttatcagat acttgacgat cactagcttc
     1201 caaagtagat tttgtttctt tagttgaatc ataagactta accttatgtt tcggttcaac
     1261 tcgttcaact tttgtatgat taccacttgt tttaatcact ggattatctt tttgaataac
     1321 tttataaact ataaaagcca tcactaacac cattaaaaat aaaactattt ttctacgttt
     1381 cattaactac ctaccgtttt cactttatca gacaatttat aaccagcttt agagggatta
     1441 taaaaactat aatgctcttt ttttaattct aaagtagaaa cataacgata attccatgaa
     1501 tatggtccaa atcctctatt atctccagaa taagttgaat aattttgctc tactattaac
     1561 atatcgccat tttcaaatac atgactaacc actccagtat gcccagcact agaagtacct
     1621 gaacttgaaa aaacagctcc agaagatgga gtattttcag agcgtccacc gaatgcagat
     1681 acccaattag aaacaaccat attaccattt cccattcttt gcgttggatg cattccgcct
     1741 ttaagccata aaccatacat caaacttgct gaaagatctg tacattgacc accagtagaa
     1801 gctatcgcat tccatccact agacgttcga taaaataaac caactgactt aggatctatt
     1861 gcatactttt tcaaatcact tggtaattga tctggtttcc atgcattata agaagtgtat
     1921 ttaactttcc ctgacttatc tttaccaaat aagccctgta ccgcattact agctgaatca
     1981 caaagagata aaactgtaga atcagaaatt atatcgctct taccagacga ggacgttttt
     2041 gaaccaccac caaaactttt agaaaatttt tctgcatcaa atttatattt tgaaccatta
     2101 aatatttcat aggctttttg cccatccgca gtcttttgag ctttattaat atatgcagta
     2161 tttcctcgtt cgtaagcatt ccaaactaaa gtggcctgct tgggatcagt catctgtgcc
     2221 atctcttcaa aagaatgatt tccacctgat aaatccattg ctttattcca gtctcctgat
     2281 ttaatggatt tcataacaaa ggcattcatg gcatctgcat cttcccattt gggatcgcct
     2341 aatggggcat actttgtgaa aggtgtgatt tgataaatac cacctccagc ttcagtcgta
     2401 tagtaagata acccagtagg tctcacatta aaggcaattg aatttgtttt taaatcatta
     2461 ccataatgcc cttctgctcg acctatcata gcaaatccac cttctgaatt aacccaacca
     2521 acaatccctg atgcactcgc accagataag cctttactta cccaagcatt gaataccttc
     2581 tctgctgttt gatttgcaac tgttcccttt tttgtccaat cactattgga agcattctca
     2641 cttgaggttt caacttgtga agatacatct ggtgtaattt gacaatcttg ttgagaactt
     2701 gacgaagcag tcaacaataa aaccaataaa aaaggaaata caatcagtat tcccaaacag
     2761 cctaattttt tcttatgttt tactttagtt ccctcctttc aaacaaaata aaaaagcacc
     2821 aacaaaaatt gatactttaa accactatga aatatgatat aataatgaag cagaaaagtt
     2881 aagacggtgg ctcttatttc tgaaaggtag gtggtcccat gggcaattca tcaaaatcca
     2941 acagaaagga ggagctagat ttgtctacct ttgaggtctt aacactcatg tttatagcag
     3001 gtaacttcgt tatcgctctc gtgaagttag tccttgaact ggtaaaatcg acaaaaaaat
     3061 aaccgtctca actttggcga gttaacggtt attttttaac ggttattttc tgccaccgtc
     3121 ttaaacggtt ctgctaagca gttgttagaa gcaactgctt tttatttaca attatattat
     3181 acataatcta aaaaatagtg tcaattaata tatagtttac taaacctctc aatgtcataa
     3241 aactccttgt actacttacc ttctctcagc ttttctagtt cttccaaaac ttttgactgt
     3301 cgttcaagat tatgttgctt tctaacttcc ttagttttac tggcattaaa tcccttttca
     3361 aaagcatttt taccatgatt ttttgtcatg gatacatcac tattaaattg acttaattca
     3421 ccctctagct tattaagctt tcgctgtttt aatggtgaag tatagttatt tgaatgattt
     3481 tctatatcat ttgttatcat atcctgattt gttaatgaag tatctccatc tccttcttga
     3541 aacgtgtgat tatccgactt aacatttttt aaaatttcct ctgttgaatt accttctgat
     3601 ataaacgata gctcataaga agattccttt cccaattgtt caatcctttt acttgcttct
     3661 ttaggtaact ctgattgata ttcaccttca ttagtcagct cagcctcttt agaaataaca
     3721 gtatcatccg cattagcttc atcatactta tcagcatcat tagttttaga ctgaccatca
     3781 tctaaattag cttgttgtct ttctttgaaa gcattccaat tatcttgtgc attcatcatt
     3841 gtaccaacac catagccagc tcccatcatg gcaaggcttg acatatcgtg tgccctctca
     3901 taaatggatt ttggagtaat acccaaatca tttgcttttt catttattct atctgcagat
     3961 tgattaataa cttgagaagc tctagaccct ataatcagtc ttaagaaagt ttctttatat
     4021 ttccaaattc cccaaaacac acaaccttgg acgaaaactg ttattagcaa cataaacagt
     4081 attgcctgac ctttaaaatt actaccacta agcagggaac catctgtaaa ttttttctta
     4141 acaaaggtgg ctattaaagt ttgagtatag aagattatca aagttaaaaa accagctaag
     4201 gcaggaaagg aaacagcccc aaacatgact ttcaaaacat tgaaaatgat atcttgcatt
     4261 ctaggtaaaa atgaaactaa taaagctaat ggaaaaataa acattaatat aatcactaaa
     4321 acatctgcca taaaagcaat taactgaatg agtattaatg gaactgcgag tataaccgct
     4381 tcaaaaatct ttaagataac atagcctgat tttatccaaa gataatcatt tactgcagaa
     4441 agccatctat ttttttcctc cccttcggtt gccttatctc ccaaattatc agtataattc
     4501 aatatatcct tttgttttgg agtgataaat ttacctgatt tatcgtactt acctaaaact
     4561 tgttcgttat caaacttttc ttctttacct gtttgattat tgtgaaactt accatttaac
     4621 tgaccagtat tgacaaacaa ataagccgta taagaagttt tcatcacata attatcagct
     4681 actgatgatt tttgtgtgat ctttgtttta ccacctgatg gattatccag tgataaatta
     4741 gttaccgcat ctgaaaacga accagctagt tgatgaacgg tatctaaaat ataaatacca
     4801 cctgatgttg attgaatcgt accaaagtaa cccataccta aaatgataac cacgaacaag
     4861 tggatcaaac gtttagaaaa atctcctttt gaaaaaagat atgaaataaa tattgaaaaa
     4921 gctgaaatcg caaccaataa ataaagcaag gagcttgtat aagacccatt acctgataga
     4981 ttttcccata atttttgaga cgaatgataa actgtttgtt tataggtatc atacaaactg
     5041 aaattttcaa aaaagcgtag aataacagaa aataaaccaa caatcaagtt aagtaggaaa
     5101 aagggaaaat taacaaaaac atctaacaaa atgctttgag ctccagtctc agctccgtga
     5161 gttatctcca tataagcctt taacgcaaat atatccacat cttttacctt cgcaaaggtc
     5221 gaagcaatca aaccatcatc tattttgtcc atctgattac ctcccttcta cgaagcatat
     5281 ttattttcaa ggtggctaga aactgttttc ttcattggtt ttaacaatgg atcaatatct
     5341 ggatgaatgt tatgtatcga aatcatattc aaattgccat aaacatcata gtagagacat
     5401 tgcccagaaa tcatattatc aatccactct aaattcttag gtgtcacctc taaccctaca
     5461 tgttttaaga tatcttcacg ttccgatttt tcataaaatg aaaaaattgt cccaaagccg
     5521 gtcgtatcat catcgttttc tgcatcatga acagattgag acactagcat caacacatta
     5581 tacttagagc gaccaacacg gcgcatagat ttaataacag cttttccttc tgatgactgc
     5641 atgagaaccc aagcctcatc aaaaatttca actgtttcat catcactacg ctcaccaaaa
     5701 tgcttacaaa acgcacctaa agcaaacatt aaagcaatcg aattactttc atgatcagat
     5761 atatgatctg aaccgtcctt aggcagtgat aaatccgcaa cttctaaaac tgtcacacgt
     5821 tcttcgtaag aaagaccagc aacatcacca tctgaaaatg ccaactctaa aatagaattc
     5881 tgaataatag ctttaaaata acgaccaact gactgaactt catcactttc agaatcaata
     5941 agtgcctcta tcacctgatt aaaaccaacg acttcaccag cttcacgttt tgcaatgact
     6001 tgactaattg cttctgttaa agctgttgtt tgatctaatt taatttctgt cgcatttttc
     6061 aacaaataaa gcaacatatt tttagccgtt gatgaagcac cttccttatc aagaataaca
     6121 ataggatcta atacaccatg attttctttg acagaactat caagagtgac aaaattcgtt
     6181 tcctcaattt gcttcttacg tagaggaaac ttacgtgcat actctggatc agaaacaact
     6241 tttaaatagt gttgccgtag ttctcgttta gggtcaatat aaaggacacg aacattttgt
     6301 tgagcagtat gtaaaaagat catctgtgct aagtatgatt tcccttgacc tgtcgcccca
     6361 gttataatca cgtgaggatt tttagtaacc ttaccagcaa catcttcttt attcgcgact
     6421 gtcgcattaa ataagacaag atttttagat cccataatag cttcgtcaat agaatcccaa
     6481 gcagttaaac gattatctac tcgtccaata taccaaccaa tcctgttacc tgattgggta
     6541 tttgtaaaaa gcatcaactc cgaaaaacct cgagcagtta ctaaatgatt ccacttacga
     6601 gttgtttttt gtaaatcttg accataaagt aacgcttgaa ataaatatgg tgtatcatga
     6661 ctggcttcat aaacattgac tttcatatca tcaaagtaac ttaaaatggc atatctacgt
     6721 tgttttagtt gatttaaact agaaccagca actaccaaat aacaaccata ctctatgatt
     6781 tcttccttat ttccaacttt tttcataaga tccttaagcg aatatgctcc catcaagata
     6841 tcatcttgtt gaaccgtatt tgtgttataa gcttctttca tgatttgatc atagcgtgta
     6901 tttgagcgcc ccatagtgcc acccaatttt gtcttatcta taaattccgc ttgaaatctt
     6961 aattcaaccg gaaaactcat tctttgaact agttcaccaa gatgaaagcc attaaatatg
     7021 gtagagaacc gacctactgg aagaacggat acaaatgaac tcccataagc actctccaat
     7081 tttaaaaaac caccttctag tgatttaatc aacgtatctg tcacatttaa catattccga
     7141 ttagctataa cctcactttt agtatgagga acatatctta aaaattgcat ccgttgataa
     7201 taaaataaat caacatccgt taatcttttg gctttcaggg tagacaataa actatagaca
     7261 agtaattctt cttctgaatg ttctttatac caatcttcat ctacttctac ctcataacca
     7321 gctaaagaag cgaagtttga tgcgatagat tcaaactgat tataaataaa ttcttttata
     7381 ttagcattgt attgcccttt accaagatca actccaatta accaatcaaa ctgatacggt
     7441 atctccattt catgtgttaa cgtatcaact gtaaaatcga gtgtttctaa ccctaattct
     7501 ttaacatcat ctgctagatc cgcttcaaaa tccgacattt tttcaccaag aagataatct
     7561 tttggtatta atgaaatatc aaaattttta tatttagcta attttttgat aacctgtgct
     7621 accttcgctt tatgactatc ttttttatca ctatctgtaa tagtgattgg tgtatttgga
     7681 atgcgatagt atgcgacaac aactttatct tttcgtaaag ctaaattttg atgtattcta
     7741 tcaatcggat attccaattt ttttaccatc tgatctcctt tcttagtctt tatattttcg
     7801 tcttctcaaa aactatttcc tctacaatgt caacacgttc tccttgataa atcccttttt
     7861 tatttaaccc aaaatctttg agataaacca gataatccca caaaaagata tgcatctttt
     7921 ttccttgcgg ttcatattct acataaaatt tcgctaactt gcttggaaca aaccaataca
     7981 agagcattga aacggaatgc gttaaagcat ttaacacctg cattaatgga gaacataccg
     8041 tcagcatcaa cactaagatg ataaagaaaa caataaaaaa tgataactgc ataggtgtag
     8101 aaaaatacca aatcactttg ccttttgccg ttttaacttc ctgaatccag taaggagcat
     8161 tcaagcccct gctgtaatca aaatattttt cttcttccat aacttataac cccgtctcaa
     8221 caccaaacca gcgaagcatt ccacccaaaa aagatagaat ctgttgacct ttaagcattg
     8281 atacaataat cgcataaata gctaaggttg agaaaacttt taaccaatta gcttctttcc
     8341 aattcttaat aattataaag acagcaatta cactaatgag gtaaatccca tcaccttgaa
     8401 aaaattgctt tagtcctgaa acgttgggac cacctgctaa aataacacta ttcactttta
     8461 ctaaattcat tttttccctc tcttacacct ctatttaaaa taatgataca tctcttcaac
     8521 ataccaacca ttgtctgctt tagataaata taatgtaaag ttttctgatc gtgtgccacc
     8581 tgttacaaga tcttcaaaag ttactgataa ttgcacgact tgatgctttt tttcttgata
     8641 ataaatcgca ttattaagat ctagctcact aactttaaat cctttcccca tcagttctgg
     8701 agttttcatt aacagtttca agtctgtctt gttacttaag gcatatttat taaagaaaac
     8761 tggtaaaaac ttttgaatag aacttacttg ttctttcgat agttccttga cttgatctaa
     8821 tgttttcttc tcaaatgctt tagatttacc taaatacaaa tcttctgcca cagtataagg
     8881 ttgactaacg attgcaagca atcctctttg catctgaaaa ggaacagcca agttcatctg
     8941 atatgatttt ttatcaactt cataaccaat acgcatcaaa gctatatagt aatctttttc
     9001 tttttcaaca cttattaaac gttgtgtctg caaagtccta gactttctaa catcatctgt
     9061 catactggca gttgagaaag aataataatt ttctaattct gtttttcttt gatctgcagt
     9121 atcttgtgaa tagttgatat agtagtagac aaaattattc atgtaatact gaattctaga
     9181 tatatctaat gaatcgtcag ttttcttatg ttttgacttt ttttctacag caataactgt
     9241 ttcttttaaa tgttgtacct gattagataa gccaattgcc ctcaatgatc caaaaaatcc
     9301 tacaataaat aacaaaccta agataccata gacaataaaa ttggctgtcc tttgctttac
     9361 ttcttttgtc ttaggctgtt tacctccctc ttctttcttt gtatatctca atagaaactt
     9421 ttttattttt tgtatatcaa tcatttaacc cctttctata aaataaaaaa gcagtcactt
     9481 tttttagcga ctacttctac ttataatgat ctctaaaaga taaaaaatag atttctccat
     9541 cttctatcat ataaatcaac ctatgttctt ctgtaattct tcgagaccaa gcccctgaat
     9601 aatggtattt taaaggttct ggttttccaa tgccttcaaa tgggtctctt ttaatatctt
     9661 ctatcaaacg attaattcgt ttcaagattt ttttatcttc ctgttgccaa gaaacataat
     9721 ctttccatgc ttcttcagta aaattaaaca tcttctacct caattaaacc atgtttagtg
     9781 acttgcttct ctttaacttg tgatatacca ttaataacct tatgtgatag ataatcattt
     9841 tccatcaaac gaatcgtttc ttgtaaactt tcccagctat cttgagataa aaccactata
     9901 ttttcatcag gatttttatt aactacaatc aaaggctcaa attcatcatt gaccttcttc
     9961 atataatctt ttaaattatt tctaaaatgt gaataaacaa tagcctccat attaatcacc
    10021 tcaactttac tagatatgtt gttattatat catttgacaa catgttgtac aagttttggt
    10081 tttgataatc actaaataaa taacttacaa atgataacaa tacataaatt atcgttaagc
    10141 ttccaattac ctcctcattc tgtttttaac acgtaaaaac cttttgaggt gtttaatact
    10201 attccttctt caatataagc atttaaagca tttacccaag ttactgaccg ttgccaaaaa
    10261 tcaattaaag aatcaaattc aactttcctt aagatatatt tcattacttt attttcatta
    10321 tttgacaaaa atatataata ttttgtcact attgaatgaa ctgataaacc ttttttcaaa
    10381 agatattgaa caatatagtc atcaccatca taaagtcctt caatacatct ttttaaaaga
    10441 tagacttctg taagtgacat aatcctatca acattccaat cagaaaaacc acaaataaaa
    10501 aaggcatcat ccttaatgcc tttttctatc atacggtttc ttacttgatt ttccgtaaac
    10561 tgcatgatgt ctaactcaca taaaaattga cttctacctt tttcacgagt ccttataatt
    10621 gcaatcatta tctacttacc ctgatatttc gacatatccg tagtctgaaa aaccaccttg
    10681 gctttctaag tcaatatctc gaccataagc ttcatgatca aaatacatct ccagagcctc
    10741 tttaccaact ccttgtacgc ctaattcatc gaaataatat tcacctagtt ctcgtctagt
    10801 tgtgacatca gacagtaaac aacaatcacc aaaattaaaa tcataatcca caaagtctga
    10861 ggcagtataa cctattacct gatgcactgc tttaacaagt tcactatcaa cattctctac
    10921 cagttcaaca aattgattaa tcatctcaat agaatcatgt tcttttagta actgacaatc
    10981 cgaatctgca ataatatact ctaaatcatc ttcttcacga agccccaaag ctaacttaag
    11041 ctcactcatt tccacaggaa aacaaactaa cgtagctcta ccagttttta tatctctaac
    11101 aattgcacta acatccattt ttgacactcc ttaattaaaa aaggagcaag ccattgactt
    11161 gctctcaccc ttaatgaaat tcttgtagtt tatgaccaat cgctgacata gacttatgag
    11221 ccttattaat catattattt tggtagtagt atgatttcaa atgttgttga atcatgtcga
    11281 ccatataacc tggtaaagtc aattcttcac gctttttaag taagctattt aactctccag
    11341 ctttttccca tgactgttca tactgatggc tgaccaacag ctctgaaagc tcctgagtaa
    11401 ccactaacaa ctttttatct gcttctgaac gtgtatttga catttttctt ttcctctttt
    11461 tctattgaat attaataatt tacaaattgt ttctaacttt agttggactt ctaataaaca
    11521 taatttcctc ccaaaataaa aagagtaggg gtagcctact tacttttgta ttttttattc
    11581 ttttaaactc aaaccaaatt ctataatctt ttgataatca tatttcttta caaaattttc
    11641 aaaaaccgaa acagcagaca aaatcttttc tgctttacgg ctatattcat cagtttgaaa
    11701 cattactctt tctaattcaa tttgttgtgg tgttccaaaa agttccgttg ccgagacatt
    11761 gaaatatcga gcaattttat ccaagtttga aaatgtagga tagccttttt gattttcaat
    11821 atttgaaatg gtttgcggta acattcctat ttttttggca agttcttttt gagatagacc
    11881 tttttgtttg cgtaaacgaa ttaaattaag tgcaaaattt tcaatcatct gttaccccca
    11941 ataaaaaatc tgtagttgtt ttaaataatt ttgctagctt gacgacattt tctaaattcg
    12001 gttcactttt ccctgtttcc caatcactat aagtattttg ttttatccct agtagtggag
    12061 ctatctcttg ctgttttaat ccactttctt gccttaaact tttaagtctc tttggaaata
    12121 taaaattaga tttgaagtca gtcacgttca tcacctctaa aattttctct tagctttaat
    12181 aatcttttat gtaaacgata ctgactagct aaattcataa caatttcttt ttgcctatct
    12241 agtggttgca tgagtaaccc aaactttaaa agtttccgtt taaaatcagc aagttccctt
    12301 aacttatctt cgtagtcttt ttgaatttca taaacaccaa cataaccaat cttaatcaaa
    12361 tcataaatgt ctatctgata atctctaagc tcacctgttt gaatctcagg ataataaaac
    12421 tcacgatcag gaatcataaa aactagtaaa tcaatcattt tatctaaatt atttcgtcta
    12481 ttaacaaaat aagcaaattc tcggttacta tacaaaacaa aacttacctc ctacaaaata
    12541 cctaagctag ctttgatact gtcaagtatc ttctccccac gttcattgac ttccccagaa
    12601 ttaagaatgg tttgtaaata gtcaccatcc acaatcatgt catactctct tatcatagct
    12661 aaagacggag aaacagaatc acttaaccaa cgtaaggttc gttcaatact ataagcctct
    12721 ggctttgtaa caaattttaa aggctcaaca cctccaaaca ttaattgcca ctttctatct
    12781 gcttgaaacg acccatattc atttttccca tcatacacgt caatcttact aacaattaac
    12841 cctctggata tttcaccaat cggcactcca gaaataaatt catcaactgc cgcattagct
    12901 ttactttgag ataaccgtat ctcataacga ttccacaatt caaaaatttc taaagcttct
    12961 tcaacagtaa ttccttcctg ctttgcaatt tcataacgtt tttcataaaa gttaaaatac
    13021 atttctgatt gacgactacc aaaataaagc gatattcctt gtctattctg ctcaatctct
    13081 tccatatccg aaaaatttaa agaaccgcca cctatataat tccaagttct caaactttca
    13141 aaatctaact catgacgata atattttgaa atcatatctg acaaatgaaa ctgttcattt
    13201 tctctatcct tacccagata caattcatca atagcaatat caaaacgtgt cacattcata
    13261 tcatcacgat atgaatttct tagataactt aaccaatcat gccatgtaaa tttttcagtt
    13321 tccatcaaca attccaattg tcggcatcca cgaccagaca attgaacagt tatttgaaaa
    13381 ttacctgtct catgtttatc cgcaaaatca aatatccaaa tatctccacg tttccaaaga
    13441 tgattgtaat tcatcaactt cgattcaaaa ggctgaaatt ctttaaatgc acagtgcaaa
    13501 aagttacgac agaaaaattc aagatcccta acatccttta acgtaattct gacatagtca
    13561 atcatcactt gtaatcgtga actatcattt tctactagat atccaaaatg tgattttatc
    13621 tcttgatact tttcaagtgt caacttccgc tttccttgct catagtttct aagtgatttc
    13681 aatgtcaaac cagaacttaa agcaaattcc ttttgtttta gacctgtttt ctttcgaaat
    13741 ttttttaaat agatagcatc cacaataaac aaactccttc ttaggattag tgacccactt
    13801 ttttcactaa aatgacccac ctttaaaaat ctgaaaaccc ttgatacata taggtttttg
    13861 ggggttaaaa ttcgaaattt ttcatttctt acccccctat tagaaaacgg gggttttaaa
    13921 attgataaat ccctctgtaa agccctagtg tgtgcgctgt gtccggcgtg tccgttttcg
    13981 ctacgctaca ctacgcctac agctacacta gggctttaca gaaggatatt taaaaaatac
    14041 ccttcatcta ctattcaccc ttaaaataat tcaatagtaa ctctttccag ctaccctcaa
    14101 attcatcttt ttgtgtcagt aaactttcta agacgactgc atcagaatca tttaatacaa
    14161 ccttttcatc ttcacgacta aaagtcatat aattatcttt ttcaatcata tctattaaat
    14221 tcttaatctt accaaatgaa aaacctgttt tcttagctaa atcaccaata ctcatatttt
    14281 ccatttcctc catttcatca tcaaaatcaa accctactga acccttaagt aactcatttt
    14341 cagacgtttt ttccacaaac tctctcaaag attcgtcttt aagaatcgcc ttagaaactt
    14401 cctgattttc tgtaggatca aaaggattct catgcctatt tattttttca aaagcatcat
    14461 aaaaagagaa atttttaggt aataaaggag aataaaattc tcttgctact tcaccaaaaa
    14521 cagctgaata acctcgacca taaacacgac gacctgcaag atatttaata aacctaaatt
    14581 ccttatttct attttcatca ccaaacatca tcacataacc accatcatcg agacgaccaa
    14641 ccgagatatg gtgcatcata ttagatctaa ttttagtggg aagatcatct gcagacggct
    14701 tttgcattgc tataatcgca ttacatccaa cctgacgtcc taacaatata tattgagtaa
    14761 atgcattatc tacaatctca cgttcctgat aagacaaaga actcattaaa gcattatatt
    14821 catcacatac aaaaaaataa ggttccaatc cataatcata aaactttttc atgtctttat
    14881 gacctaaacg cttcatttca tttcgaacaa aatcataacg tgcaaacatt atcgtaacag
    14941 cattttcaaa cttctcaata atatcagcct tttcaaaggc aattctacct tcaaaagctg
    15001 ataaatctga catagtaaca aaatcggctc ttttagggtc acatatatca caaactccaa
    15061 tttccgcaag acaacgaagg attgacctta gtaatactgt tttacctcca ccagtaccac
    15121 cagcaactaa taaatgagga tcattgatga aatcccaata atagccatcc attaacttaa
    15181 ttcccttatc tttatcccat atcacatctt taaccgtaat acgtgataag aaagccgaat
    15241 aagctaattt gtaaatttta aagcgtggat catcagtttt ttccataaaa tccatgaaaa
    15301 aagtatcttc taattctcca ccaatatcct taaatttctt ttgaaatttt cctccagcca
    15361 tttcaaatga aacacttaaa tcatacttac cttgtttcac ataaactttt ggaaaacgat
    15421 attttgtctt cgttttctta tttgattttt tacgtttttc ataaacatag ccattctcat
    15481 ataaaaactt agacattctt tttaatcgat ctagtttatt aaataatgga ttagcttctt
    15541 gcaaaaacca agtcaaatac caagaaacaa aaaatgctaa aaacaaacct gtacctacac
    15601 tgatcataga cattttatcc atcattttta acaaatctaa atagtaataa ctatacacgc
    15661 ctacaggtgt caaaaacaaa ataaatagaa aagaaaaaag gtaataactc atatacctca
    15721 tatatggttt taccttaatc cctctatatt gtggtattaa acgagccaaa ttacccctcc
    15781 tttaccgttt atttattaac tttagatgct tcattttttg atgcgtttcc attatttgca
    15841 acatcctttt tcttaagtga tgatgcaaaa attttaaaca taccacctga aacgttagac
    15901 catgtcacaa caacatcact taattcaata gggtcaccca atttaagacc taaatcctcg
    15961 attgcataag aaggcatatc agtaattgaa acaaaaattg tttcattttg agcagtacta
    16021 aatactgcaa tatcattcca cttcacttca cctgttggtt tttgtggata acgaccaaat
    16081 cgatctggtg gttgtgtcgt atcatcttcc gtaaattgaa ctggttcttc atatcgtctc
    16141 caagttaaat ctcctaacaa aacatttaca tccaatccat cattaaaggt acgatattcc
    16201 ttatctgcaa tttgtaattt ttctccaaag tttaaaccaa tttttgccat atttctaatc
    16261 ctctactttc cttatttcac ttcaataagc ttttcagccc atacattttt tgcaggttca
    16321 atatttctac cattaattgt attgtcaata aacaataacg gtttttcaac agttaccatt
    16381 tttccatttc caatcacacg tgaagatgct ggtgtataaa cataaaactt accatgtttt
    16441 tcattaaaca aaatgtgtct tgtataagac ccttttttac catttaattg agcaggtcca
    16501 gcttcaaatc cttcataaat tgcatctcca ataaaatttg gaagtaacac taatgtttct
    16561 tgtttcataa tttcctcttt tctaaaaata agaagcactt tttcaagtgc ttctgaatat
    16621 tgacttttta attttctttg cgttttttaa atcctgctaa accgatagct gacaagatga
    16681 ttgctccaag tgctgtaaga ggggcaacgc ttgcttcacc agtttgaggt aatcctttag
    16741 caggagcttt tggagttgct ttttgtggtt caactggttt tggtttctca ggagtatgtg
    16801 ttaccacagt ttcagtttta accttattcc cattgataaa aaagtctgcg gtattgtaaa
    16861 catcacctgc tttgatacgt ttaaccacca caaaatcatc tgccccaaat tcactagaac
    16921 gtgcaacttt ttcaaggaag tctttcttga agactaattc atagagacct gtctttttgt
    16981 tgtaaactgt ttctgtgtac tctcctaaat tagtcccttt tttaatgact gtaccatctt
    17041 ttaatgtcac atcaacttta gcgaccactt tatcacgtaa gtaaagatca tgtgtgcgtt
    17101 gtaattgatc cacaaatttg tattcaaata gatcataaga acgacctgtt ggaactaccc
    17161 aaccctctaa tttgtaagta acttcatcac ctaatttgac agtaccattg ttgatagact
    17221 tgccatcttc tttgtctaac acatctttat gtatttctgg agttgtcaaa tcattgacta
    17281 caatattacc tgtatatcca ttgccaaagt cgatttgtgc aacaccattt tgaatttgac
    17341 ctttagtaaa ctctttttta actttaaatg ataagttata agtaacatct agaccttttt
    17401 gaacataagc tttataaaaa gcttgtggat cttttgcagt ccacatgtaa aactcaccaa
    17461 caggactgat tcctgcttct ttaataagcg tttgaagttt ctcatctaaa gtatccgtag
    17521 aaagcacatg tcgcatttct aagagttgtg acacatctgt gccatcagat gctttgattg
    17581 agttcacttt catagactta ccatctagtg cgtcatcttt ataatcatca ataaagacaa
    17641 agtttttagc tattttacct tgtgatgcag tcattccttt atattggcta aagtcttgtt
    17701 tagcaacgta atttaattca gcatttggta atacggtttt accattaaca gattgacctg
    17761 cttcatcaat gacagctttt gaaggattag cagctggaac taaaacagga tgaacactaa
    17821 ctgagtagtt aatcttttca ggaatagaca catgtggttt cacttttggt tgagctggag
    17881 tgtaagtttt taatgtaggt gttacaaatt taggctcaac tggtttttca ggtactggtt
    17941 tttctgcttc aggagtcact ttttctgttg acggtggtgt tggctgtttt ggtataacaa
    18001 cgttaggaac aacatcatta ttattaacat aagacactaa agcagaccca tgtgtgtcat
    18061 gtacccagtc atcatgaatc ttaaagactt caacgcttcc actagaacca tgtggtttaa
    18121 gctcatattt aagattgagt tgtaaagttt tgtttaaagt ttctgcaaaa gttttgttac
    18181 cattatgttt cttctcaaaa atgatttgtc cgtctttagt taagactctg aaagcaatat
    18241 tttgattaac agcagcattt tgagcaacat tacgatactc gtacttgtaa agagtgatag
    18301 agtcaacaaa tgctgtcaca gagccatctt tgttagcaac ataatgatga ttaactttac
    18361 ctgtcaattt cccatagccg attgtcttca caccatcgat catatcatgg ttaaactcga
    18421 aacttccgtc aggtttagtt tttgcagtca tgattgagtt tccatattca gcgttatcaa
    18481 cacctttagc ttgagctttg atatcaaaag caacattatt aatagactgt ttatctgctt
    18541 ctgctttagc ttttagagct tgattataaa gtgctaattt agcttgatag tctttttgta
    18601 actgtgcgtt agtcgcatct gctttagctt gtgcatcttt attacgttga cgtacacctg
    18661 cattataagc ttcaactgat ttgattttcg ctaatttagc ttgataatct ttttgtaatt
    18721 gtgcattagt cgcatctgct ttagcttgtg catctttgtt ttcagcatca atttttgctt
    18781 tagtttgtgc atttaactga cttacacgtt gagactctgc tttgttttct gcgtcaattt
    18841 ttgcattttc agcagtagca tctttaatag cttgtgtatt agcttgtgct tttgcagtaa
    18901 catctttaac tgtttgtgtt tgattagcta aatcagcttg tgcatcagta gttgtcgcat
    18961 gattaactgc agctgtggtt gtaacagcta cagctgcttt tttagctgta gctactgctt
    19021 tatcaagttg agttgatgtt actgtttctg cttgattttg cgttactgtt tgagttgttg
    19081 cttgagttgt aacttgatca gcagacactt gactagtagc taatgtaaat gcacctgcaa
    19141 gtgcaatccc acatactaaa ccatatgctt ttgattttct aaaaaatccg tgaccttttg
    19201 tgttttgatt tttatacata ttttctcctt tgattttaat tttttatata aaaaaaagaa
    19261 ggcgatcttg ccctca
//
LOCUS       DBJYSH010000030        19154 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.030, whole genome shotgun sequence.
ACCESSION   DBJYSH010000030 DBJYSH010000000
VERSION     DBJYSH010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 19154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19154
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.030"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            92..2725
                     /locus_tag="ACUBV3_000829"
     CDS             92..2725
                     /locus_tag="ACUBV3_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HHU3847347.1"
                     /translation="MKGQKIIALAGLVLSCHFALTACHTQEHKNSYHIKTKQVAKKKA
                     NKKKVSVKESHKKRKGVAGVDFPTDDGFLLTKDSKILSHTDSGIVVAHGNHSHFIFYS
                     DLKGSKFSYLIPNANAKTNKNQAVRNFKAGAVAVNTLNDGYVFNPADIVSEDANGYVV
                     RHGDHFHYIPKASLSQQKQVQASRAVSRLGNQSNSHYRVNSSKIAGLHYPTSDGFLFN
                     GRGIKGTTPTGILVEHHNHLHFISFADLRKGGWGSIADRYQPQKKADSKKQSPSSKKP
                     RNENTLPKDIKDKLAYLARELHLDISRIRVLKTLNGEIGFEYPHDDHTHVIMAKDIDL
                     SKPIPNPHHDDEDHHKGHHHDESDHKHEEHEHTKSNKLSDENQKKLIYLAEKLGLNSN
                     QIEVLTSEDGSIIFKYPHDDHSHTIASKDIEIGKPIPDGHHDHSHAKDKVGMATLKQI
                     GFDDEIIQDILHADAPTPFPSNETNPEKMRQWLATVTKINIGQRTNPFQRFGLSLMPN
                     IEVLGIGFTPINDMTPVLQFKKLKQLWMTNTGITDYSFLDKMPLLEGLDISQNGIKDL
                     SFLTKYKQLSLIAAANNGITSLKPLAELPNLQFLVLSHNNISDLTPLSNLTKLQELHL
                     DHNNVKNLSALSGKKDLKVLDLSNNKSADLSTLKTTSLETLLLNETNTSNLSFLKQNP
                     KVSNLTINNAKLSSLDGIEESDEIVKVEAEGNQIKSLVLKNKQGSLKFLNVTNNQLTS
                     LEGVNNYTSLETLSVSRNKLKSLDIKTPNKTVTNLDFSHNNVPTSQLKLNEKNIPEAV
                     AKNFPAVVEGSMVGNGSLAEKAAMASKEDKQVSDNTNHQKNTEKSAQANADSKKENPK
                     THDEHHDHEETDHAHVGHHHH"
     gene            2825..3484
                     /locus_tag="ACUBV3_000830"
     CDS             2825..3484
                     /locus_tag="ACUBV3_000830"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HHU3847348.1"
                     /translation="MTMVKREDVILNATENMVYHKLKNDPSGHDWFHIVRVRNLAVEL
                     AHKEGANTFICQMAALLHDIIDDKICQDSKQASYELTQWLYSQDLAIAEVEHILDILE
                     NISFKAGTGLTMKTLEGQIVQDADRLDAMGAIGIARTMAYSGSKGRLIHDPNLKPREN
                     LTLEEYRNGQDTAIMHFYEKLLKLKDLMNTKQGKMLAQKRHDFLELYLAEFYAEWNGK
                     G"
     gene            3493..3957
                     /locus_tag="ACUBV3_000831"
     CDS             3493..3957
                     /locus_tag="ACUBV3_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000621138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HHU3847349.1"
                     /translation="MIRRAKEKDLPAIAELLKQILVLHHGVRPDIFHTRGSKFSKEQL
                     KEMLMDESRPIFVYESDEGKVVAHLFLQLQEKRDLPRKSFKTLYIDDLCIDEEVRGQQ
                     IGQKLMDFARQYAKKHGCYNITLNVWNDNQRAVSFYEKLGFKPQQTQMEEIL"
     gene            3957..4394
                     /gene="msrB"
                     /locus_tag="ACUBV3_000832"
     CDS             3957..4394
                     /gene="msrB"
                     /locus_tag="ACUBV3_000832"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HHU3847350.1"
                     /translation="MKETQEELRQRIGHTAYQVTQNSATEHAFTGKYDDFFEEGIYVD
                     IVSGEVLFSSLDKFQSGCGWPAFSKPIENRMVTNHQDHSHGMHRIEVRSRQADSHLGH
                     VFNDGPVDAGGLRYCINSAALDFIPYDQMAKRGYGDYLSLFDE"
     gene            4543..7335
                     /locus_tag="ACUBV3_000833"
     CDS             4543..7335
                     /locus_tag="ACUBV3_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HHU3847351.1"
                     /translation="MASTNNVSDTNTRFAKEELEEVFEELGTTQGGLSDEEVAVRQKK
                     YGLNLLSEVKQESIILLFLKNFTSLMAILLWVGGFVAIVSNSLELGLAIWMVNVINGI
                     FSFIQEYRASQATQALKKMLPSYSRVLRKSSEEKVLSEQLVPGDIVLIEEGDCISADG
                     RLIKTTDLQVNQSALTGESNPIYKDSNVENYQSKTLIECDNMVFAGTTVSSGSATMVV
                     TAIGMQTQFGQIADLTQGMKSEKSPLQRELDRLTKQISIISITVGIIFFLAATFFVKE
                     PVSKSFIFALGMIVAFIPEGLLPTVTLSLAMAVQRMAKEHALVKKLSSVETLGATSVI
                     CSDKTGTLTQNEMTVNHLWQNGKSYQVTGLGYAPEGQILFEGDNICFGNSDRGDLEKL
                     IRFAHLCSNAQVLPPNDDRSTYTVLGDPTEACLNVLLEKSGINIQENRKFAPRLKELP
                     FDSVRKRMTTIHSLGGDEKDKKISITKGAPKEILDLSDYVLSDGKVIPLNKEERNKIQ
                     LANDTFAKDGLRVLAVSYCDIEGFSKEQWTQENLEQHMVFIGLIAMSDPPREGVREAI
                     DKCHAASIRIIMVTGDYGLTALSIAKNIGIIRNDDAKVISGLELSEMTDSQLKKELSG
                     EVVFARVAPEQKYRVVTILQEMGEVVAVTGDGVNDAPALKKSDIGVAMGVTGTDVAKE
                     SADMILTDDHFASIVHAVEEGRAVYQNIKKFLTYIFNSNTPEAVPSAFFLFSKGFIPL
                     PLTVMQILAVDLGTDMLPALGLGVEPPETDVMNRPPRRLTDRLLDKGLLIKSFLWYGT
                     IESVLAMGGFFWAHYLRYGNFTFFVANGIPYREATTMTLGAIIFSQIGMVMNSRTSYQ
                     SIKTLSIFGNKLINFGIIMEILAFLVLVYVPLFHNLFNTASLGLSHWLYLISCPFIMI
                     GLDEVRKLFSSRKNKR"
     gene            7335..8438
                     /locus_tag="ACUBV3_000834"
     CDS             7335..8438
                     /locus_tag="ACUBV3_000834"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HHU3847352.1"
                     /translation="MSNIITYLKNNSNLTFDELALNDVDILCLNEFGYISFEKLINTT
                     EMKSVLVCELYHEYLQTMAKSYSFMFTSQRHDLCQLMMTSKRFKNLTLSYYQAEISLE
                     FEKQFAAMVFTIPNIDYHQVVFRGTDANLIGWKEDFKLTYMREISAHRSAIKYLNTIL
                     PYFDKVVLSGHSKGGNLALYAAMFTKPDLKAKIDLIWLIDSPGLQKTLLPTTEYKTTK
                     QKCIRLLPEESIVGMMLYSDIEPLIISSNARGILQHDVTTWEIQEPAILKTGAGLSLK
                     SICFEKTFQQWMAELKSQERKLFFDLLFDSFLSSGVSSLDDFNLASRAKMMKAFHSFR
                     ELDDDKKRLFNKSLKLLVTIFWGAYHDNSRETK"
     gene            complement(8556..9194)
                     /locus_tag="ACUBV3_000835"
     CDS             complement(8556..9194)
                     /locus_tag="ACUBV3_000835"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HHU3847353.1"
                     /translation="MASILKKHLEKRLELKNDPMRTDMSTGASYPKYGFSVGKYTYGY
                     QQFFYEGVNLKEIGAFCSIAQNVTITGMNHPTDHITTNPFIYYKSRGFINEDRADLID
                     EKKNGKVIIGNDVWIGTNVTILPSVTIGNGAIIEAGSVITKDIPDYAVVAGTPAKIIK
                     YRFSEEEITLLNASQWWNWSDEAIKEHISEFSDKREFFNTLKSISENKNHKL"
     gene            complement(9463..9812)
                     /gene="ssrA"
                     /locus_tag="ACUBV3_000836"
     tmRNA           complement(9463..9812)
                     /gene="ssrA"
                     /locus_tag="ACUBV3_000836"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            9905..10516
                     /locus_tag="ACUBV3_000837"
     CDS             9905..10516
                     /locus_tag="ACUBV3_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001056395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HHU3847354.1"
                     /translation="MNMKLSKRYRFWQKVIKALGVLALIATLVLVVYLYKLGILNDSN
                     ELKDLVHKYEFWGPMIFIVAQIVQIVFPVIPGGVTTVAGFLIFGPTLGFIYNYIGIII
                     GSVILFWLVKFYGRKFVLLFMDQKTFDKYESKLETSGYEKFFIFCMASPISPADIMVM
                     ITGLSNMSIKRFVTIIMITKPISIIGYSYLWIYGGDILKNFLN"
     gene            complement(10553..11335)
                     /locus_tag="ACUBV3_000838"
     CDS             complement(10553..11335)
                     /locus_tag="ACUBV3_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter (permease)"
                     /protein_id="HHU3847355.1"
                     /translation="MFGKLLKYELKSVGKWYLTLNAAVLLVSIILGLVLKALGGNFST
                     DTNSTSAQIFTIILVLLLAMVISGSLLSTLAIIIKRFYSNIFGRQGYLTLTLPVTTNQ
                     IICSKLLASLLWSIFNIFIVIIGIILVILPLVGIGQFVVAFPEIYKIISSSNAPLFIA
                     YFFLSYVAGTLLIYLSIAVGQLFTNKRVLMGIVSYFGISLLITFLTLIIDSIFHIDLF
                     NSHANATFSQPVLLYNILVSIVEIAIFYMLTHSIIKYKLNIQ"
     gene            complement(11347..12045)
                     /locus_tag="ACUBV3_000839"
     CDS             complement(11347..12045)
                     /locus_tag="ACUBV3_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HHU3847356.1"
                     /translation="MTQLLQLHHVTKKYHKHIAVNDVTVSIPTGKIIGLLGPNGSGKT
                     TIIKMINGLLQPDKGDIVIDGYRPSVETKKIISYLPDTSYLQENMKIKDVVTLFEDFY
                     NDFNSKVAYQLFEDLNLNPKERLKNLSKGNKEKVQLILVMSRKARLYILDEPIGGVDP
                     AARDYILKTIISNYSNDASVLISTHLISDIEPILDEVIFLKEGEIDLQGNADDLREEH
                     NCSIDALFRERFKK"
     gene            complement(12046..12414)
                     /locus_tag="ACUBV3_000840"
     CDS             complement(12046..12414)
                     /locus_tag="ACUBV3_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HHU3847357.1"
                     /translation="MAWEFNEKSPIYSQIAEHIKMQIVSQEIKSGDQLPTVRELAQEA
                     GVNPNTMQRAFTELEREGMVFSQRTSGRFVTEDNLLIGKIRQQVAKAELATFVNNMKK
                     IGYKLDEITVALDHFIKEIN"
     gene            12559..15663
                     /locus_tag="ACUBV3_000841"
     CDS             12559..15663
                     /locus_tag="ACUBV3_000841"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HHU3847358.1"
                     /translation="MFAQLDTRTVYSFMDSLVDLKTYVSKSKSLGYQTIGILDHSNLY
                     AAYHFIQEAQKANLRPIVGFSFDIVVENRSIEVYCIAINTVGYKNLLKLSTAQMSEKM
                     SLNLLTEHLEGVQLILPYQDALDQLNLPFDYVIGVNLTSPQIPYTKPIIAIDTVRYFQ
                     KNDIETLQMLHAIRDNVLLKDAQYASKNQELKPCQEMTLAFKKRFPEALANLESLVEN
                     VVYHFDSDFKLPIFNREIPAGEELRTLTQNNLKSKGLWSDAYQERLEKELTIIHKMGF
                     DDYFLIVWDLLRFGRSKGYYMGMGRGSAAGSLVSYALNITGIDPVKHNLIFERFLNEE
                     RYSMPDIDIDLPDIYRGEFLRYVRNRYGSMHSAQIVTFSTFGAKQAIRDVFKRFGASE
                     YELTNITKKIHFRDNLTSVYNRNLAFRQIIDSKIEYQKAYDIAKRIEGNPRQTSIHAA
                     GVVMSDDLLTDHIPLKNGEDMMITQYDASSVEANGLLKMDFLGLRNLTFVQKMKEKVD
                     KDYGISIQLEAIDLEDKETLKLFAAGQTKGIFQFEQSGAINLLRRIRPECFEDVVATT
                     SLNRPGASDYTENFINRRFGKEKIDLVDPVIAPILQPTYGIMLYQEQVMQIAQTYAGF
                     TLGKSDLLRRAMSKKNSKEMQKMSQSFLEGAVSKGHRQEDARLIFERMAKFAGYGFNR
                     SHAFAYSALAFQLAYFKAHYSDVFYDIMINYSNSDYLIDAIDFGFVIEKPSINTISYR
                     DRIYKKKIYLGLKNIKAVPNDLAYWISKNQPFQSIEDFLMRLPQQFQKSGFISPLIAI
                     GAFDEFDNNRRKITSNLDSLFTFVNELGSLFADTSYHWLEVEDFSNSEKYEMEQDILG
                     VGISPHPLVGISQKASRPFIPISQVQENSEATILVQLKQVKIIRTKSSGQQMAFLTVM
                     DINSKMDITVFPETFNIVRDDLQEGKYYYLHGKIQKRDERLQMVLNGVQEATEERFWI
                     LLKNHDNDKKISEILSKYKGHIPVYLHYETTKETIQSKVHLVRKDSGLALDLSEFVVK
                     TVYQ"
     gene            15744..16766
                     /gene="pfkA"
                     /locus_tag="ACUBV3_000842"
     CDS             15744..16766
                     /gene="pfkA"
                     /locus_tag="ACUBV3_000842"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HHU3847359.1"
                     /translation="MKRIAVLTSGGDAPGMNAAIRAVVRKAISEGMEVYGINQGYYGM
                     VTGDIFPLDANSVGDTINRGGTFLRSARYPEFAELEGQLKGIEQLKKHGIEGVVVIGG
                     DGSYHGAMRLTEHGFPAVGLPGTIDNDIVGTDYTIGFDTAVATAVENLDRLRDTSASH
                     NRTFVVEVMGRNAGDIALWSGIAAGADQIIVPEEEFNIDEVVSNVRAGYAAGKHHQII
                     VLAEGVMSGDEFAKTMKAAGDDSDLRVTNLGHLLRGGSPTARDRVLASRMGAYAVQLL
                     KEGRGGLAVGVHNEEMVESPILGLAEEGALFSLTDEGKIVVNNPHEADLRLAALNRDL
                     ANQSSK"
     gene            16815..18317
                     /gene="pyk"
                     /locus_tag="ACUBV3_000843"
     CDS             16815..18317
                     /gene="pyk"
                     /locus_tag="ACUBV3_000843"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018366040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HHU3847360.1"
                     /translation="MNKRVKIVATLGPAVEFRGGKKFGESGYWGESLDVEASAEKIAQ
                     LIKEGANVFRFNFSHGDHAEQGARMATVRKAEEIAGQKVGFLLDTKGPEIRTELFEDG
                     ADFHSYTTGTKLRVATKQGIKSTPEVIALNVAGGLDIFDDVEVGKQILVDDGKLGLTV
                     FAKDKDTREFEVVVENDGLIGKQKGVNIPYTKIPFPALAERDNADIRFGLEQGLNFIA
                     ISFVRTAKDVNEVRAICEETGNGHVKLFAKIENQQGIDNIDEIIEAADGIMIARGDMG
                     IEVPFEMVPVYQKMIITKVNAAGKAVITATNMLETMTDKPRATRSEVSDVFNAVIDGT
                     DATMLSGESANGKYPVESVRTMATIDKNAQTLLNEYGRLDSSAFPRNNKTDVIASAVK
                     DATHSMDIKLVVTITETGNTARAISKFRPDADILAVTFDEKVQRSLMINWGVIPVLAD
                     KPASTDDMFEVAERVALEAGLVESGDNIVIVAGVPVGTGGTNTMRVRTVK"
     gene            18488..19045
                     /gene="lepB"
                     /locus_tag="ACUBV3_000844"
     CDS             18488..19045
                     /gene="lepB"
                     /locus_tag="ACUBV3_000844"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001931829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HHU3847361.1"
                     /translation="MVKRDFIRNIILALIAVLILILLRYFVFATFKVHKDATNSYFSN
                     GDVVVVNRNRTPKYKDFIVYKVGKIFYISRVIGEPNQKVRVMDDILYLNDVFKDEPYI
                     EKMKNAYSEKKDGQMPFTSDFSVETLTRNKESRVPKGSYLVLNDNRQNKNDSRKFGLI
                     KEKDIRGVITFKVYPLSEFGFTASE"
BASE COUNT         6405 a         2989 c         3638 g         6122 t
ORIGIN      
        1 aataaaaaga tggcaaatag tattgcaaaa agaaaaacat ctgatataat taactagtta
       61 actaaaacga aaaaacattt atggaggact catgaaaggt caaaaaatta ttgctctagc
      121 tggtcttgtc ctgtcatgcc attttgctct tacggcatgt catactcaag aacacaaaaa
      181 ttcttatcat attaaaacaa agcaggttgc taagaaaaaa gctaataaaa agaaagtctc
      241 tgttaaggaa agtcataaaa aacgcaaggg tgttgcaggg gttgactttc caactgatga
      301 tggcttttta ctgactaaag attcgaagat tttatctcat acagattctg gaatcgttgt
      361 agcacatgga aatcattctc attttatttt ttactctgat ttaaaaggtt caaaatttag
      421 ctatttaatc cctaatgcta atgcaaaaac taataaaaat caagcggtaa gaaactttaa
      481 agcaggggcg gtagctgtta atacattaaa tgatggctat gtttttaatc ccgcagatat
      541 tgtttcagaa gatgctaatg gctacgtggt cagacatggt gaccatttcc actatattcc
      601 aaaagcaagt ttatctcagc aaaagcaagt acaagcaagt agagctgttt cacgtttagg
      661 aaatcaaagt aatagccatt acagggttaa tagttctaaa attgcagggc ttcactaccc
      721 aacaagtgat ggtttcttat ttaatggacg aggaataaaa ggcactaccc caacaggtat
      781 tttagtagaa catcataatc atctgcattt tattagtttt gctgatttaa gaaaaggggg
      841 gtggggatca attgctgacc gttaccaacc acaaaagaaa gctgattcta aaaaacagtc
      901 accatcaagt aaaaagccaa gaaatgaaaa tactttacct aaggacatca aagataagct
      961 agcttacctt gctcgtgaat tacatttaga tatttcacgt atcagagtcc ttaaaacact
     1021 aaatggtgaa attggatttg aatacccaca tgatgatcat acacatgtta ttatggccaa
     1081 agatattgat ctaagtaagc caattccaaa tccacatcat gatgacgaag atcatcacaa
     1141 aggtcatcac catgatgagt ccgaccataa gcatgaagaa cacgagcaca ctaaatcaaa
     1201 taaattatct gatgaaaatc aaaagaaatt aatttattta gcagagaagc ttggtttaaa
     1261 ttctaatcaa attgaagtat taacgtcaga ggatggaagt atcatcttta agtatccaca
     1321 tgatgaccac tcacacacta tagcaagtaa ggatatcgaa attgggaagc ctatcccaga
     1381 tggacaccac gatcactctc atgcaaaaga taaagttggt atggcaactc taaaacaaat
     1441 tggttttgat gatgagatta ttcaggatat tctgcatgca gatgctccaa caccattccc
     1501 atcaaatgaa actaatcctg aaaaaatgcg tcagtggctt gcaactgtta ctaagattaa
     1561 cattggacaa agaaccaatc cattccagcg ttttggtcta tcattgatgc ctaatattga
     1621 agttctagga attgggttta ctccgattaa tgatatgacg ccagttttgc aattcaaaaa
     1681 acttaaacag ctttggatga caaatactgg tattacagat tattctttcc ttgataagat
     1741 gccactatta gaaggattgg atatttcaca aaacggtatt aaggatttat ctttccttac
     1801 aaaatataaa caattaagtc ttattgctgc agctaataat ggcattactt cgctaaaacc
     1861 attggctgaa ttaccaaacc ttcaattctt agttttgagt cataacaata tctctgactt
     1921 gacaccgtta tcaaacttga caaaactcca agaattgcac cttgatcata ataatgtgaa
     1981 gaatttaagt gcactttctg gtaaaaaaga tttgaaagtt ctagatttat caaacaataa
     2041 atccgcagac ttatcgactc taaaaacaac tagtcttgag acgcttcttt taaatgagac
     2101 gaatacaagc aatctaagtt tcttaaagca aaatcctaag gtatctaatt taacaattaa
     2161 taatgcgaaa ttatcatcat tagatggtat tgaagagagc gatgaaattg ttaaagtaga
     2221 agctgaaggg aaccaaatta agtcactagt attgaaaaat aaacaaggtt cgttaaaatt
     2281 cttgaatgtg actaataatc agttaacatc acttgaaggt gttaataatt atacttcact
     2341 tgagacctta agtgtttcta gaaataaact taaatcttta gatattaaaa cacctaacaa
     2401 aacagttaca aatcttgatt ttagccataa caatgttcca acatcacaat tgaaattgaa
     2461 tgaaaaaaat attcctgaag cagtagctaa gaattttcca gcagttgtag aaggttcaat
     2521 ggttggaaat ggtagtcttg ctgaaaaagc agctatggct tctaaagagg acaaacaagt
     2581 ttcagataat actaatcacc aaaaaaacac tgaaaaatct gctcaagcga atgctgatag
     2641 taaaaaagaa aaccctaaaa cacatgatga acatcatgac catgaagaaa cagatcatgc
     2701 acatgtaggt catcatcacc attaatcttt tacggagcta atatataaaa tccttcaaga
     2761 atttcttgga ggattttgca tttatatgct aaatcattta tttttttaga aaattagcta
     2821 taatatgact atggttaaaa gagaagatgt gattttaaat gcaactgaaa atatggttta
     2881 ccataaatta aaaaatgatc caagtggcca tgactggttt catattgtta gagtaagaaa
     2941 tcttgcagta gaattagcac acaaagaagg tgctaatacc tttatttgtc aaatggctgc
     3001 acttttgcat gatattattg atgacaaaat ctgccaagat agtaagcaag cgtcgtatga
     3061 attgactcaa tggttatata gtcaagatct tgccatagca gaagtagaac atatccttga
     3121 tattcttgaa aatatttcat tcaaagcagg aactggattg actatgaaaa cattggaagg
     3181 tcaaattgtt caagatgcag atcgcctaga tgctatgggg gctattggta ttgcaagaac
     3241 gatggcttat tcagggagta aaggacgttt aattcatgac cctaacttaa aaccacgaga
     3301 aaacctcact ttagaagagt atcgtaatgg tcaggataca gctatcatgc atttttacga
     3361 aaaattatta aagttaaaag accttatgaa tacaaaacaa gggaaaatgc ttgctcaaaa
     3421 acgtcatgat tttttagaac tatatttagc cgaattttat gctgaatgga atggtaaagg
     3481 ttaggagtag atatgattcg tagagcgaaa gaaaaagatt tgcctgctat tgcagagcta
     3541 ttgaaacaaa ttttagtgtt acatcatggg gttcgcccag atatttttca tacaagagga
     3601 agtaagtttt ctaaagaaca attgaaagaa atgctgatgg acgaatcaag gcctatcttt
     3661 gtttatgaat cagacgaggg taaagttgtt gcccacttgt ttcttcaatt acaagaaaaa
     3721 cgtgacttac ctcgaaagtc ctttaaaact ctctatatag atgatttgtg cattgatgag
     3781 gaagtaagag gtcagcaaat tggacaaaag ttgatggatt ttgcaagaca atatgctaag
     3841 aaacatgggt gttataatat aactttaaat gtttggaatg ataatcagag agcggtctct
     3901 ttttatgaaa aattaggttt taaacctcaa caaactcaaa tggaggagat actataatga
     3961 aagaaacaca agaagaatta cgtcagcgta tcggacatac agcttatcaa gtaacccaaa
     4021 attcagctac ggaacatgct tttactggga agtatgatga tttttttgaa gaggggatat
     4081 atgtagatat cgtcagtgga gaggttttat tctcttcttt agataaattt caatctggtt
     4141 gtggttggcc agcattttca aaaccaattg agaatcgaat ggtgacaaat catcaagatc
     4201 actctcatgg aatgcatcgt attgaggtta ggagtcgtca agctgactct catttagggc
     4261 atgtatttaa tgatggaccg gttgatgcag gtggcttacg ctattgtatc aattcagctg
     4321 ccttagattt tattccttat gaccagatgg caaagagagg ctatggggac tacttatcac
     4381 tttttgatga ataagagacg acttaagggt atatcttaag tcgtttttta tgtatggtaa
     4441 aaagaattgg tggcaggtgt attcttcatc agtttatttt ctgaaaaaag agtataatat
     4501 aggtatcaaa aatgaaaacg ttttagagaa ggagactaga atatggcttc tacaaataat
     4561 gtttcagata caaacacacg ttttgctaaa gaggaattag aagaggtttt cgaagaatta
     4621 gggacaactc aaggtggttt atcagatgaa gaggttgctg tacgtcagaa aaaatatggt
     4681 ttaaacctgt tatctgaagt aaagcaagaa tcaattattt tattgtttct gaaaaatttt
     4741 actagtctta tggcaatact attatgggtt ggaggatttg tggctattgt ttctaatagc
     4801 ttagagctag gtcttgccat ttggatggtt aatgtgatta atggtatttt tagtttcatt
     4861 caggaatacc gtgcaagcca agcaacacaa gctctcaaga aaatgttacc atcttattca
     4921 cgtgttctta gaaaaagctc agaagaaaag gtattatcag agcaactagt cccaggagat
     4981 attgttctta ttgaagaagg agattgtatt tcagcagatg gtcgcttgat taaaacaaca
     5041 gatttgcaag taaaccaatc agctttgaca ggggaatcaa atcctattta taaagatagc
     5101 aatgttgaaa actaccagtc taaaacattg attgaatgtg ataatatggt tttcgcagga
     5161 acgacagtct ccagtggtag cgcaacaatg gttgttacgg caattggaat gcaaacccaa
     5221 tttggtcaaa tcgctgattt aacacaaggg atgaaatcag aaaaaagccc tcttcaaaga
     5281 gaacttgatc ggttgaccaa acagatttct atcatttcaa ttactgtagg aattatcttt
     5341 ttcttagcag cgactttttt tgttaaggag ccagtgtcaa aatcatttat ctttgccttg
     5401 gggatgatcg ttgcatttat cccagaaggt ttactaccta ccgttacact ttctctagct
     5461 atggcagtcc aacgaatggc taaagaacac gcacttgtca aaaaactttc ttcagttgag
     5521 actcttggag caacctcagt tatctgttct gataagactg gtacattaac gcaaaatgaa
     5581 atgaccgtca atcatttgtg gcaaaatgga aagagttacc aagtgacagg actgggttat
     5641 gctccagaag gacagattct ttttgaaggt gacaatattt gctttggtaa tagtgataga
     5701 ggagacttag aaaaacttat tcgttttgct catctctgta gcaacgctca agtattacca
     5761 ccaaatgatg atcgttcaac ttatactgta cttggagatc cgacagaagc ttgtttgaat
     5821 gttttattgg aaaaatcagg gataaacata caagaaaatc gtaagtttgc accacgtttg
     5881 aaagagttac cttttgattc ggttagaaaa cgcatgacaa ctattcatag cttaggaggc
     5941 gatgaaaagg ataaaaaaat ttcaatcaca aaaggtgcgc cgaaagaaat acttgattta
     6001 tcagattatg tcttatctga tggcaaagtt atccccttaa ataaggaaga acgtaataaa
     6061 atacagttgg ctaatgatac atttgctaaa gatggacttc gcgtcttagc tgtttcatac
     6121 tgcgatattg aaggcttttc aaaagaacaa tggacgcaag aaaatttgga acaacacatg
     6181 gtgtttatag ggttgatagc aatgagtgat ccacctcgtg aaggcgttcg tgaagctatt
     6241 gataaatgtc atgcagcaag cattcgtatt attatggtga ctggagatta cggattaacg
     6301 gcactgagta tcgctaaaaa tattggtatt attcgtaacg atgatgctaa agtaatttca
     6361 ggtttagagt tatcagaaat gacggatagc caattgaaaa aagaattgtc aggtgaagtc
     6421 gtttttgctc gtgtagcacc agaacaaaaa tatcgagtag taacgatatt acaggagatg
     6481 ggtgaagtag tagcagttac aggagacggg gtcaatgatg cgcctgcgct aaaaaaatcc
     6541 gatattggtg tagccatggg tgttaccggt actgatgttg ctaaagaatc agctgatatg
     6601 attctgacag atgatcattt tgcttctatt gtacatgccg tggaagaagg gcgggctgtt
     6661 tatcaaaaca ttaagaaatt cttgacgtat atttttaata gtaatactcc tgaggcggtt
     6721 ccgtcggcat tcttcctttt ttctaaaggt tttattcctt taccattgac tgtaatgcaa
     6781 atacttgctg ttgatttggg aaccgatatg cttcccgctt taggtctagg cgtggaacca
     6841 ccagaaacag atgttatgaa tcgtcctcca agacggctaa cagatcgctt attggacaaa
     6901 gggctactta taaaatcttt cctttggtac ggcactattg aatccgtatt agctatgggt
     6961 ggttttttct gggctcatta cttacgttat ggtaatttta cttttttcgt cgcaaacggc
     7021 ataccgtaca gagaagcgac aactatgaca cttggagcta ttatctttag ccagataggt
     7081 atggttatga atagcagaac gagctatcaa tctattaaaa cactatctat ttttggtaat
     7141 aaattaatta attttggtat aataatggag attttagcat ttttagtctt agtatacgtg
     7201 ccactattcc ataacttatt caacacagca agcctaggtt tatcacattg gctctattta
     7261 ataagctgcc cgtttatcat gataggttta gatgaagttc ggaaattatt cagcagtaga
     7321 aagaataaac ggtgatgtca aatataataa cttatttaaa aaataattca aacttaactt
     7381 ttgatgaatt agcattaaac gatgttgaca ttctttgttt aaatgaattt ggctatattt
     7441 cttttgaaaa acttataaac actacagaaa tgaagtcagt tttagtctgt gagttatatc
     7501 atgagtacct tcaaactatg gctaaatcat atagctttat gttcacgtca caacgacatg
     7561 atttatgtca gttaatgatg acctcgaaac gttttaaaaa cttaacttta tcttattatc
     7621 aagctgagat tagtttagaa tttgaaaaac aatttgcagc tatggttttt actattccta
     7681 atattgatta tcatcaggtt gtatttcgtg gtactgatgc caaccttatt ggttggaaag
     7741 aagattttaa attgacgtat atgagagaaa tatcggctca tcgttcagcg attaaatact
     7801 taaatacaat actgccttat tttgataaag tagttctctc aggtcattct aagggtggta
     7861 atttagcctt gtacgctgca atgtttacaa agccagattt aaaagcaaaa attgatttga
     7921 tttggcttat tgactcccca ggactacaaa aaactttgtt acctactaca gagtataaaa
     7981 ctactaaaca aaagtgcatt cgcttactac cagaagaatc tattgttggt atgatgcttt
     8041 atagtgatat agagccgcta attattagta gcaatgcgcg tggaattctg caacacgatg
     8101 ttacaacttg ggaaattcaa gaaccagcta ttttaaagac tggagcagga ctatcgctaa
     8161 aaagtatttg ttttgaaaag acatttcaac agtggatggc tgaattaaaa agtcaagaaa
     8221 gaaaattatt ttttgattta ttatttgata gcttcctatc aagtggcgta tctagtttgg
     8281 atgacttcaa cttggcttcc agagcaaaaa tgatgaaagc ttttcatagc tttagagaac
     8341 tagatgatga taaaaaaagg ttatttaaca aatctttaaa attattagta accatatttt
     8401 ggggagctta tcatgataat agcagagaga ctaagtagct ttcctagaat tgcattagtc
     8461 taaacatgtg ctaaataaac tagcaaaata gcatactaaa atactactca tattatgctg
     8521 agtagtattt tagtgtgcta tttgatgaag tcattctaga gtttatgatt tttgttttca
     8581 gaaatacttt ttagagtatt gaagaattcc ctcttatctg aaaattcgct aatatgttcc
     8641 ttaatagctt catctgacca attccaccac tgagatgcat ttaataatgt tatttcttcc
     8701 tctgaaaagc gatatttgat aattttagca ggtgttccag cgacaacagc atagtcagga
     8761 atgtctttgg taataactga acctgcttca ataattgctc cattacctat tgtaacagat
     8821 ggaagtatag tgacattagt tcctatccag acatcgttgc caataataac ttttccattt
     8881 ttcttttcat ctattaagtc cgccctatcc tcatttataa atccccttga cttatagtaa
     8941 ataaaagggt tagtggtaat gtgatcggtt ggatgattca taccagtaat agtaacgttc
     9001 tgggctattg agcaaaatgc tccaatttct tttaagttaa cgccttcgta aaagaattgt
     9061 tgatatccat atgtgtactt tcctaccgag aatccatatt tagggtaact ggcaccagta
     9121 ctcatatctg tcctcatagg atcattttta agctctaaac gtttctctaa atgttttttt
     9181 aaaatactag ccataactag cctccaatcg taggctaaat agattataac attttttcct
     9241 ctaaaaacct tatctttttt aataaattct ataaattaga atgataaaga acttattctc
     9301 ggatattgac aaagagtgag ctaatgaggt ggcctaattc aatgttagag tctgttccaa
     9361 gtattttttt cctaagatag gggcaatgta tgagcaaaga aacaaaataa aaaccttgta
     9421 ataacaaggt tttaagtatg tatagtaagt tatgataaaa tgatatggag ccggtgggag
     9481 tcgaacccac gtccaaacat ctgctaatat atttgtctac aactataggt tatgtattga
     9541 tttaactagg tgatgacaca taactcaagc cctcacatag cgagtctata aatctctttt
     9601 taactaacta gacagtagcc aaacgtagtt tgctgataaa taagaccttc tagcaaaacg
     9661 caaacaatct tgtgaagatc acgcaggctg ttttttaggc agctaatgcg taagaatttg
     9721 tattttttgc agttatattt aactggcatt ttacatctgc cgatgagtcg caaaatatac
     9781 ctcataatgc ctgtcgaatc cgtaacgacc ccaaaagtag aataaagcat aaaaacttac
     9841 caatatagta taacaaatac tatttaaaat agcaaaaatt taacagttgt gttatgatta
     9901 aagcatgaat atgaaattat caaaacgtta ccgcttttgg cagaaggtca ttaaggcttt
     9961 gggtgtctta gcgttaatag cgacccttgt cttagttgtt tatttgtaca aacttggtat
    10021 tttgaatgat agcaatgaat taaaagacct agtacacaag tatgaatttt ggggtccaat
    10081 gatttttatt gtggctcaaa ttgtacagat tgtttttcca gtaattcctg gaggtgttac
    10141 aacagtagca ggtttcttaa tttttggtcc taccttaggc tttatttata actatattgg
    10201 gattatcatc ggaagtgtca tccttttttg gttagtcaaa ttttatggac gaaaattcgt
    10261 tttgctattt atggatcaga agacatttga taaatatgag tctaagctag aaacatccgg
    10321 atatgaaaag ttttttatct tttgtatggc atctcccatt tcaccagcgg acatcatggt
    10381 gatgattacc ggtctatcta atatgtctat taagcgcttt gtgacaatca ttatgataac
    10441 gaaacctatt tctatcatcg gttatagcta tttgtggatt tatggcggtg atattttgaa
    10501 aaattttttg aattaaaaat aaaaaaaggt tggagatata tctccaatct ttttattgta
    10561 tattaagttt gtacttaata atagagtgtg tcaacatata gaatatagca atttcaacaa
    10621 tgctaacaag tatattgtag agcaatacag gttgagagaa tgttgcattt gcatgagagt
    10681 tgaataaatc aatatgaaaa atactatcta ttattaatgt taagaaagta attaagagtg
    10741 atattccaaa ataagaaaca attcccatca atacacgttt gtttgtaaat aattgcccta
    10801 cagcgataga gagataaatc agtaatgtcc ctgcaacgta cgatagaaag aaataggcga
    10861 taaaaagtgg agcgttcgaa gatgaaataa ttttatagat ttctggaaaa gcgacaacga
    10921 actgaccaat acctactaaa ggtaatatga ccaatatgat tccaataatg acaataaaaa
    10981 tattaaaaat actccaaaga agcgaagcta gtagctttga acaaataatc tgattcgtag
    11041 ttactgggag cgttaaggtt agatatcctt gacggccgaa gatattgcta taaaaacgtt
    11101 tgataataat agcaagggtg gataacagag atcctgaaat aaccatcgct aacaataaaa
    11161 cgagtattat agtgaatatt tgggcgctag tactattagt atcagtagaa aaattgccgc
    11221 caagtgcctt taataccagt cctaaaataa tagatactaa taaaaccgca gcgttgagtg
    11281 ttagatacca cttaccaact gattttaatt catattttaa taattttcca aacatatcaa
    11341 tctcctttat ttcttgaatc tttcacgaaa gagagcatca atcgaacagt tgtgctcttc
    11401 tcttaagtca tcagcattgc cttgtaggtc aatttctcct tctttaagga agataacttc
    11461 atcaaggata ggttcaatgt cagaaattaa gtgagtcgaa attaaaacag aagcatcatt
    11521 agagtagttg ctaatgattg tttttaaaat gtagtcacgc gctgcagggt ctacaccacc
    11581 aataggttca tcaaggatgt agagcctagc tttacgactc ataacaagta ttagttgaac
    11641 tttctccttg ttcccttttg ataggttttt tagacgttcc ttaggattta ggttaagatc
    11701 ctcaaatagt tgataagcta cctttgaatt aaaatcgtta tagaaatctt caaataatgt
    11761 aacgacgtct ttgattttca tattttcttg taaatatgaa gtatcaggga gatatgaaat
    11821 aatttttttg gtttcaacag acggtctgta accatcgata acaatatctc ccttatctgg
    11881 ctgaagtaat ccatttatca ttttgatgat tgttgttttt ccgctaccat ttggacctaa
    11941 taaacctata atctttcctg ttggaatgct tacagtcaca tcattaacag caatatgttt
    12001 gtgatatttc tttgtaacat gatgaagttg taataactgt gtcatttaat taatctcctt
    12061 tataaaatga tcgagagcaa ccgtaatttc atctaattta tatcctattt ttttcatgtt
    12121 gttgacaaaa gttgctaact ctgcttttgc aacttgttga cgaattttac caattagtaa
    12181 gttgtcttct gtaacaaagc gtccggaagt acgttgtgag aataccattc cttcacgttc
    12241 caattcagtg aaggcacgtt gcatggtatt tgggttaaca ccagcttctt gagctaactc
    12301 tctcacggta ggcaattggt caccactttt tatttcttga ctaacaattt gcatctttat
    12361 atgttcagca atttgtgaat aaataggtga tttttcattg aattcccaag ccatagataa
    12421 ctcctttcat aatcttgtac tttttgtact ataaaaatag tacaaaaaaa tatttgaaat
    12481 gtcaaggaat tatcattgag actttaatcg tttattgttt catagtataa tagtaaaaaa
    12541 tcgtaaaaag gggctactat gtttgcacaa cttgatacaa gaaccgttta ttcttttatg
    12601 gatagcttgg ttgatttaaa gacatatgtc tctaaatcga aatcattagg atatcaaact
    12661 attggtatct tggaccacag taatctttat gcagcatatc attttattca agaggcgcaa
    12721 aaggcaaacc taagaccaat agttggattt agctttgata tagtggttga aaatagatca
    12781 atagaagttt actgtattgc gataaataca gttggttata agaatctctt gaagttgtca
    12841 acggctcaaa tgtctgagaa gatgtctcta aatttattga cagaacatct tgaaggggtg
    12901 caattgattc ttccctatca agatgctctt gatcagctca atttaccctt tgattatgtt
    12961 attggtgtta atttgacctc tccgcagatt ccatatacca aacctattat agccatagat
    13021 actgttcgat attttcaaaa aaatgatatt gagacattac agatgcttca tgctataaga
    13081 gataatgtcc tgttaaaaga tgcacaatat gcgagcaaaa atcaagaatt aaaaccttgt
    13141 caagaaatga ctctagcatt caagaaacgt tttcctgaag ctttagctaa cctagaatct
    13201 ttggtagaga atgttgttta tcactttgat tctgacttta agttacctat ttttaataga
    13261 gaaattcctg ctggagaaga attaagaacc ttaactcaaa ataacttaaa aagtaaaggt
    13321 ttatggagcg atgcttatca agagcgtctc gaaaaagaat taacaatcat tcacaaaatg
    13381 ggctttgatg actatttcct tattgtgtgg gatttattaa gatttggtcg tagtaagggc
    13441 tactatatgg gaatgggacg tggttcagca gcgggaagtt tagtctcata tgctttaaat
    13501 attaccggta tcgatcctgt caaacataat ttaatttttg aacgtttttt aaatgaagag
    13561 cgttattcga tgccagatat tgatattgat cttccggata tttatcgtgg agaattcttg
    13621 cgctatgtac gtaatcgata tggttcaatg cattctgcac agattgtaac cttctcaact
    13681 tttggtgcga aacaggctat tcgagatgtt tttaaacgct ttggtgcatc agagtatgag
    13741 ttaacaaaca taactaaaaa aattcatttc agagataatt tgaccagtgt ttataaccgc
    13801 aacttagctt tcagacaaat aatagatagt aaaattgaat accaaaaagc ctatgatatt
    13861 gctaagcgta ttgaaggaaa tcctagacag acttctattc atgccgctgg ggtagtaatg
    13921 agtgacgatt tgttaacaga tcatattcct ttaaaaaacg gtgaggatat gatgataacg
    13981 caatatgatg ctagttctgt tgaggctaat ggtcttctaa aaatggactt tctaggtctt
    14041 cgaaatttga catttgttca gaaaatgaag gagaaagtcg ataaggacta cggtatctct
    14101 atacaattag aagctattga tttagaagat aaagagactc taaaactatt tgcggctggt
    14161 caaacaaagg ggatttttca atttgagcaa agtggagcta ttaatttgtt gcgacgtatt
    14221 aggccagagt gttttgaaga tgtggtagct actaccagct tgaataggcc tggtgcaagt
    14281 gattatactg agaattttat aaaccgtcgt tttggtaaag aaaaaatcga tttagtagat
    14341 cctgtcatag ctccaatttt acaacctacc tatggcataa tgctctatca agaacaggtt
    14401 atgcagattg cccagaccta cgcaggtttt acgctaggca agtctgattt gctcagacgt
    14461 gctatgtcta agaaaaattc taaggagatg caaaagatgt ctcaatcctt cttagagggt
    14521 gcagtatcta agggacatcg tcaagaagat gctcgactta tatttgaacg catggctaaa
    14581 tttgcaggtt atgggtttaa tcgcagccat gcctttgctt attcggcttt agcttttcaa
    14641 ttagcctatt tcaaagccca ttattccgat gtcttttatg acatcatgat aaattattca
    14701 aatagtgatt atctaatcga tgctattgat tttggttttg ttattgaaaa accttcaatt
    14761 aatactattt catacagaga ccgcatctat aaaaagaaaa tttatttagg tttaaaaaat
    14821 attaaggctg ttcctaacga cttggcttat tggatttcta aaaatcaacc atttcagagt
    14881 attgaagatt ttctaatgag actgccacag cagtttcaaa aaagtggttt tatttctccg
    14941 ctaattgcta ttggagcatt tgacgaattt gataacaata gacggaaaat cacctctaat
    15001 cttgattctt tatttacatt tgtaaatgaa ctaggtagtt tatttgcaga tacctcatat
    15061 cattggttag aagtagaaga tttctctaat tctgagaaat atgaaatgga acaagatatc
    15121 ttaggagtag gaatcagtcc gcacccttta gtggggattt cacaaaaagc tagccgtccg
    15181 tttataccaa ttagtcaagt ccaagaaaat agcgaagcta ctattttagt tcagctaaag
    15241 caggtcaaaa tcattaggac aaaaagttct ggacaacaaa tggctttctt gactgttatg
    15301 gatattaatt caaaaatgga tattactgtt tttcctgaaa ctttcaatat tgtaagagat
    15361 gatttacaag aagggaaata ttattacttg catggaaaaa ttcagaagcg agatgagcga
    15421 ttacagatgg ttttaaacgg tgttcaagaa gcaacggagg aacgtttttg gatacttcta
    15481 aaaaatcatg ataacgacaa aaaaatatcg gaaatattgt ccaaatataa agggcatatt
    15541 cctgtctatt tgcattatga aacaactaaa gaaactatcc aaagcaaagt acatttggtt
    15601 agaaaagata gtggcttagc ccttgattta tctgaatttg ttgtgaaaac ggtttatcaa
    15661 taaaattgca tataaagtgc agaaatttac aaattaatat gctataatta aatcgttaaa
    15721 ttaataaaag gagtaccaat taaatgaaac gtattgctgt tttaactagt ggtggtgacg
    15781 cccctggtat gaacgctgct atccgtgcag ttgttcgtaa agcaatttct gaaggtatgg
    15841 aagtttacgg catcaaccaa ggttactatg gtatggtgac aggggatatt ttccctttgg
    15901 atgctaattc tgttggggat actatcaacc gtggaggaac gtttttacgt tcagcccgtt
    15961 atcctgaatt tgctgaactt gaaggtcagc ttaaagggat tgaacagctt aaaaaacacg
    16021 gtattgaagg tgtagtagtt atcggtggtg atggttctta tcatggtgct atgcgtctaa
    16081 ctgagcacgg tttcccagct gttggtttgc cgggtacaat tgataacgat atcgttggca
    16141 ctgactatac tattggtttt gacacagcag ttgcgacagc agttgagaat cttgaccgtc
    16201 ttcgtgatac atcagcaagt cataaccgta cttttgttgt tgaggttatg ggaagaaatg
    16261 caggagatat cgctctttgg tcaggtatcg ctgcaggtgc agatcaaatt attgttcctg
    16321 aagaagagtt caatattgat gaagttgtct caaatgttag agctggctat gcagctggta
    16381 aacatcacca aatcatcgtt cttgcagaag gtgttatgag tggtgatgag tttgcaaaaa
    16441 caatgaaagc agcaggagac gatagcgatc ttcgtgtgac gaatttagga catctgctcc
    16501 gtggtggtag tccgacggct cgtgatcgtg tcttagcatc tcgtatggga gcgtacgctg
    16561 ttcaattgtt gaaagaaggt cgtggtggtt tagccgttgg tgtccacaac gaagaaatgg
    16621 ttgaaagtcc aattttaggt ttagcagaag aaggtgcttt gttcagcttg actgatgaag
    16681 gaaaaatcgt tgttaataat ccgcatgaag cggatcttcg cttggcagca cttaatcgtg
    16741 accttgccaa ccaaagtagt aaataagaat ttgtcaattt gacactttaa ataaaaagga
    16801 gctcataaat tattatgaat aaacgcgtaa aaatcgttgc aacacttggt cctgcggtag
    16861 aattccgtgg tggtaagaag tttggtgagt ctggatactg gggtgaaagc cttgacgtag
    16921 aagcttcagc agaaaaaatt gctcaattga ttaaagaagg tgctaacgtt ttccgtttca
    16981 acttctcaca tggagatcat gctgagcaag gagctcgtat ggctactgtt cgtaaagcag
    17041 aagagattgc aggacaaaaa gttggcttcc tccttgatac taaaggacct gaaattcgta
    17101 cagaactttt tgaagatggt gcagatttcc attcatatac aacaggtaca aaattacgtg
    17161 ttgctactaa gcaaggtatc aaatcaactc cagaagtgat tgcattgaat gttgctggtg
    17221 gacttgacat ctttgatgac gttgaagttg gtaagcaaat ccttgttgat gatggtaaac
    17281 taggtcttac tgtgtttgca aaagataaag acactcgtga atttgaagta gttgttgaga
    17341 atgatggcct tattggtaaa caaaaaggtg taaacatccc ttatactaaa attcctttcc
    17401 cagcacttgc agaacgcgat aatgctgata tccgttttgg acttgagcaa ggacttaact
    17461 ttattgctat ctcatttgta cgtactgcta aagatgttaa tgaagttcgt gctatttgtg
    17521 aagaaactgg caatggacac gttaagttgt ttgctaaaat tgaaaatcaa caaggtatcg
    17581 ataatattga tgagattatc gaagcagcag atggtattat gattgctcgt ggtgatatgg
    17641 gtatcgaagt tccatttgaa atggttccag tttaccaaaa aatgatcatt actaaagtta
    17701 atgcagctgg taaagcagtt attacagcaa caaatatgct tgaaacaatg actgataaac
    17761 cacgtgcgac tcgttcagaa gtatctgatg tcttcaatgc tgttattgat ggtactgatg
    17821 ctacaatgct ttcaggtgag tcagctaatg gtaaataccc agttgagtca gttcgtacaa
    17881 tggctactat tgataaaaat gctcaaacat tactcaatga gtatggtcgc ttagactcat
    17941 ctgcattccc acgtaataac aaaactgatg ttattgcatc tgcggttaaa gatgcaacac
    18001 actcaatgga tatcaaactt gttgtaacaa ttactgaaac aggtaataca gctcgtgcca
    18061 tttctaaatt ccgtccagat gcagacattt tggctgttac atttgatgaa aaagtacaac
    18121 gttcattgat gattaactgg ggtgttatcc ctgtccttgc agacaaacca gcatctacag
    18181 atgatatgtt tgaggttgca gaacgtgtag cacttgaagc aggacttgtt gaatcaggcg
    18241 ataatatcgt tatcgttgca ggtgttcctg taggtacagg tggaactaac acaatgcgtg
    18301 ttcgtactgt taaataattt ttgaattcaa acctattatt taagaatacc ttaaataatg
    18361 ggttttttat atgttttgct ttttattatt cgattattgt gactagaata ggatatgata
    18421 ttaataatta ccctcctttt tttatctatg aaaacatgat ataatggaaa aaagattagg
    18481 agtagttatg gttaaacgcg attttattag aaatattatt ttagcattga ttgctgtatt
    18541 gattttgatt ctcttgagat attttgtttt tgcaaccttt aaagtacata aggatgctac
    18601 aaattcttat tttagcaacg gagatgttgt tgttgttaat cgtaatagga cgcctaagta
    18661 caaagacttt attgtctata aggttggaaa gattttctat attagtcgtg ttattggtga
    18721 gccaaatcaa aaagtgagag taatggatga tattttatat ttgaatgatg ttttcaagga
    18781 tgagccttac attgaaaaaa tgaaaaatgc ttattctgaa aagaaagacg gtcaaatgcc
    18841 ctttacttcc gacttctcag ttgaaacatt aacacgaaat aaagaaagca gagttcctaa
    18901 gggttcttat cttgtgttaa atgataatcg acaaaataaa aatgatagtc gaaaatttgg
    18961 attaatcaaa gagaaagata ttcgtggtgt tattaccttt aaagtatacc ctctaagtga
    19021 atttggtttt acagcatcag agtagtaaaa gaatggtatt atttgatacc attcttttta
    19081 tgtaataaaa tggctaagaa atatattttt ttaagataga tagaattttt gatagcgttt
    19141 atctcgttat caca
//
LOCUS       DBJYSH010000031        18912 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.031, whole genome shotgun sequence.
ACCESSION   DBJYSH010000031 DBJYSH010000000
VERSION     DBJYSH010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 18912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18912
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.031"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            complement(<1..402)
                     /locus_tag="ACUBV3_000845"
     CDS             complement(<1..402)
                     /locus_tag="ACUBV3_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3847362.1"
                     /translation="MLIKGLLSPNSKVSPKDYKVYMRVLSFVFSVCSLIMIVSSFLHT
                     FESNFTKGFSFGSGIALFAGSFYFLVITFSEKRFQHYYTKVFDERNQFIANMSCRLLF
                     LLSVLVLVVLCLINVWFGITFSYAKFLLLILS"
     gene            complement(443..580)
                     /locus_tag="ACUBV3_000846"
     CDS             complement(443..580)
                     /locus_tag="ACUBV3_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001867090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3847363.1"
                     /translation="MSRMWTIDFCPPNVYQQYVFIITISISIAMSIFAIYRYASLKKT
                     I"
     gene            complement(639..845)
                     /locus_tag="ACUBV3_000847"
     CDS             complement(639..845)
                     /locus_tag="ACUBV3_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HHU3847364.1"
                     /translation="MKNSLQKLRKSRKLSQAELAVALGVTRQTIISLEKEKYTASLEL
                     AFKIARYFDKQIEEVFIYTESERG"
     gene            complement(996..2189)
                     /locus_tag="ACUBV3_000848"
     CDS             complement(996..2189)
                     /locus_tag="ACUBV3_000848"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000047536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HHU3847365.1"
                     /translation="MSKTIAINAGSSSLKWQLYEMPEEKVVAKGIIERIGLKDSISTV
                     KFDDKKDEQILDIVDHTQAVKILLEDLTKHGIIKDFNEITGVGHRVVAGGEYFKESAL
                     VDDKVVEQVEELSALAPLHNPAAAAGIRAFREILPDITSVCVFDTAFHTTMQPHTYLY
                     PIPQKYYTDYKVRKYGAHGTSHQYVAQEAAKQLGRPLEELKLITAHVGNGVSITANYH
                     GQSIDTSMGFTPLAGPMMGTRSGDIDPAIIPYLVANDSELEDAAAVVNMLNKQSGLLG
                     VSGTSSDMRDIEAGLQSKDPNAVLAYNVFIDRIKKFIGQYLAVLNGADAIIFTAGMGE
                     NAPLMRQDVIAGLSWFGIELDPEKNVFGYFGDITKPDSKVKVLVIPTDEELMIARDVE
                     RLKAK"
     gene            complement(2221..3195)
                     /locus_tag="ACUBV3_000849"
     CDS             complement(2221..3195)
                     /locus_tag="ACUBV3_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HHU3847366.1"
                     /translation="MNFEKIETAYELILENIQTIENQLKTHIYDALIEQNSYYLGSSC
                     DLDIVVVNNQKLRQLDLSQEEWRRTFQFIFIKSAQTEQLQANHQFTPDSIGFILLFLL
                     EELTSQETVDVLEIGSGTGNLAQTLLNNSSKELNYMGIEVDDLLIDLSASIAEIIGSS
                     AQFIQEDAVRPQILKESDVIISDLPIGYYPNDDIAKRYAVSSSKEHTYAHHLLMEQSL
                     KYLKKDGIAIFLAPENLLTSPQSDLLKEWLKGYADVIAVLTLPETIFGSRQNAKSIFV
                     LKKQAEQKPETFVYPLTDLQNRENMANFIENFQKWSRENSHYSKNMIE"
     gene            complement(3310..3681)
                     /gene="comGG"
                     /locus_tag="ACUBV3_000850"
     CDS             complement(3310..3681)
                     /gene="comGG"
                     /locus_tag="ACUBV3_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGG"
                     /protein_id="HHU3847367.1"
                     /translation="MILKKKLKAGILLQAIVLAAVFTLVLQFYLARILATERQYHSQI
                     EASKAYLTAQLAYKTIEGDSISGKCYFTGGYASYLQEGNYLQVKVTLDKGGNYNHKFY
                     RKTDTLQVPLKSNDNKASHKH"
     gene            complement(3659..4120)
                     /gene="comGF"
                     /locus_tag="ACUBV3_000851"
     CDS             complement(3659..4120)
                     /gene="comGF"
                     /locus_tag="ACUBV3_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001880689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGF"
                     /protein_id="HHU3847368.1"
                     /translation="MKVIRKFCTYQKLRVKAFTLLECLVALVTITGALLVYQGLTKLL
                     AQQIVVMSSSSQSEWVLLTQQLNAEFEGAHLEYLRQNKLYLRKQDKIVTFGKSNKDDF
                     RKTGYDGRGYQPMVYGLDNCQMSQTKSMVKLVFYFKDGLKRTFYYDFKEET"
     gene            complement(4074..4373)
                     /gene="comGE"
                     /locus_tag="ACUBV3_000852"
     CDS             complement(4074..4373)
                     /gene="comGE"
                     /locus_tag="ACUBV3_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGE"
                     /protein_id="HHU3847369.1"
                     /translation="MVIIVRKKIRAYILLESLVGLAILVTITSLVISEINRQQSHIAR
                     RQHEQEVLILARMAVQTDQKILEANGVNIRIEESKNKIVLFEGNKEILHVSKVKG"
     gene            complement(4345..4773)
                     /gene="comGD"
                     /locus_tag="ACUBV3_000853"
     CDS             complement(4345..4773)
                     /gene="comGD"
                     /locus_tag="ACUBV3_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGD"
                     /protein_id="HHU3847370.1"
                     /translation="MKNLLLKCKDKKVKAFTLLESLIVLSVVAFMTLVFSTSFNNIFR
                     QVEETIFFISFEHLYRDTQKLSAFGQKKQTLTISHNYLENTYERLYLPKTVKVVKSDT
                     LAFDANGGNSSLAKIQFECYRKTVTYQLYIGSGNYRKKEN"
     gene            complement(4770..5813)
                     /gene="comGB"
                     /locus_tag="ACUBV3_000854"
     CDS             complement(4770..5813)
                     /gene="comGB"
                     /locus_tag="ACUBV3_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011074699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus assembly protein ComGB"
                     /protein_id="HHU3847371.1"
                     /translation="MDKWISWLKKDISVRNRHKSKKLSLKKQRKVVQLFNNLFASGFS
                     LTDMVTFLKRSKLLSDYYTDRMNKALLEGKDLSKMLGELGFSDTVITQVALADLHGNI
                     SRSLLKIESYLANLLLVRKKVIEVATYPLILLTFLVLIMIGLRHYLMPQLGENNFATS
                     LITNVPNIFLLLLAVVLIFSLIFYIVQKRLSRIKVACFLTTIPLVGSYVKLYLTAYYA
                     REWGNLLSQGVELDQIVKVMQNQKSKLFREIGYDMEEGFLSGKAFHQKVLDYPFFLTE
                     LSLMIEYGQVKAKLGTELDIYADEKWEDFFTKLARATQLIQPVIFIFVALIIVMIYAA
                     MLLPMYQNMEILS"
     gene            complement(5707..6678)
                     /gene="comGA"
                     /locus_tag="ACUBV3_000855"
     CDS             complement(5707..6678)
                     /gene="comGA"
                     /locus_tag="ACUBV3_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000250804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus ATPase ComGA"
                     /protein_id="HHU3847372.1"
                     /translation="MVQSLAKQVIHQAVEVNAQDIYIIPKGDCYELYMRIDDERRFID
                     VFEFNRMASLISHFKFVAGMNVGEKRRSQLGSCDYELSEGRLVSLRLSSVGDYRGQES
                     LVIRILYSGHQDLKYWFDNIKQMKEVLGTRGLYLFSGPVGSGKTTLMYQLASEVFKNK
                     QIITIEDPVEIKNDKMLQLQLNEDIGMTYDALIKLSLRHRPDILIIGEIRDQATARAV
                     IRASLTGVMVFSTIHAKSISGVYDRLIELGVNYQELENSLKLIAYQRLIGGGSLIDFE
                     TGNFKKHSSDKWNRQVDILAEEGHISKKQAQVEKIIPQETTESSPTF"
     gene            complement(6851..7216)
                     /locus_tag="ACUBV3_000856"
     CDS             complement(6851..7216)
                     /locus_tag="ACUBV3_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HHU3847373.1"
                     /translation="MYQVVKMFGDWEPWWFIEGWEEDITEIAEYDTLSEALLYFQEEW
                     DRGQEKWPYFQSKSSLLATFWSIKEKRWCEECDEYLQQYHSLMLLKEWQEIPKEESIE
                     RFEVFNKIAELPSACSLNL"
     gene            complement(7330..10980)
                     /gene="rpoC"
                     /locus_tag="ACUBV3_000857"
     CDS             complement(7330..10980)
                     /gene="rpoC"
                     /locus_tag="ACUBV3_000857"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HHU3847374.1"
                     /translation="MVDVNRFKSMQITLASPSKVRSWSYGEVKKPETINYRTLKPERE
                     GLFDEVIFGPTKDWECACGKYKRIRYKGIICDRCGVEVTRAKVRRERMGHIELKAPVS
                     HIWYFKGIPSRMGLTLDMSPRALEEVIYFAAYVVIDPMDTPLEPKSLLTEREYREKLQ
                     EYGYGSFVAKMGAEAIQDLLKRVDLDAEIAVLKEELKSATGQKRVKAVRRLDVLDAFK
                     KSGNKPEWMVLNILPVIPPDLRPMVQLDGGRFAASDLNDLYRRVINRNNRLARLLELN
                     APGIIVQNEKRMLQEAVDALIDNGRRGRPITGPGSRPLKSLSHMLKGKQGRFRQNLLG
                     KRVDFSGRSVIAVGPTLKMYQCGVPREMAIELFKPFVMREIVARDLAGNVKAAKRMVE
                     RGDERIWDILEEVIKEHPVLLNRAPTLHRLGIQAFEPVLIDGKALRLHPLVCEAYNAD
                     FDGDQMAIHVPLSEEAQAEARLLMLAAEHILNPKDGKPVVTPSQDMVLGNYYLTMEDA
                     GREGEGMIFKDHDEAVMAYQNGYVHLHTRVGIAVDSMPNKPWTEEQKHKIMVTTVGKI
                     LFNDIMPEDLPYLIEPNNANLTEKTPDKYFLEPGQDIQAVIDNLEINIPFKKKNLGNI
                     IAETFKRFRTTETSAFLDRLKDLGYYHSTLAGLTVGIADIPVIDNKAEIIDAAHHRVE
                     DINKAFRRGLMTEEDRYVAVTTTWREAKEALEKRLIETQDPKNPIVMMMDSGARGNIS
                     NFSQLAGMRGLMAAPNGRIMELPILSNFREGLSVLEMFFSTHGARKGMTDTALKTADS
                     GYLTRRLVDVAQDVIIREDDCGTDRGLTITAITDGKEVTETLEERLIGRYTKKSIKHP
                     ETGEILVGADTLITEDMAAKVVKAGVEEVTIRSVFTCNTRHGVCRHCYGINLATGDAV
                     EVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVASNTDITQGLPRIQEIFEARNPKGEAV
                     ITEVKGEVVAIEEDSSTRTKKVFVKGQTGEGEYVVPFTARMKVEVGDEVARGAALTEG
                     SIQPKRLLEVRDTLSVETYLLAEVQKVYRSQGVEIGDKHVEVMVRQMLRKVRVMDPGD
                     TDLLPGTLMDISDFTDANKDIVISGGIPATSRPVLMGITKASLETNSFLSAASFQETT
                     RVLTDAAIRGKKDHLLGLKENVIIGKIIPAGTGMARYRNIEPLAVNEVEIIEGTPVDA
                     EVTEVSTPTTED"
     gene            complement(11097..14672)
                     /gene="rpoB"
                     /locus_tag="ACUBV3_000858"
     CDS             complement(11097..14672)
                     /gene="rpoB"
                     /locus_tag="ACUBV3_000858"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HHU3847375.1"
                     /translation="MAGHEVQYGKHRTRRSFSRIKEVLDLPNLIEIQTDSFQDFLDAG
                     LKEVFEDVLPISNFTDTMDLEFVGYELKEPKYTLEEARIHDASYSAPIFVTFRLVNKE
                     TGEIKTQEVFFGDFPIMTEMGTFIINGGERIIVSQLVRSPGVYFNDKVDKNGKVGYGS
                     TVIPNRGAWLELETDAKDIAYTRIDRTRKIPFTTLVRALGFSGDDEIVDIFGDSELVR
                     NTIEKDIHKNPSDSRTDEALKEIYERLRPGEPKTADSSRSLLVARFFDPRRYDLAAVG
                     RYKINKKLNLKTRLLNQTIAENLVDGETGEILVEAGTVMTRDVIDSIAEHIDGDLNKF
                     VYTPNDYAVVTEPVILQKFKVVAPTDPDRVVTIVGNSNPEDKVRALTPADILAEMSYF
                     LNLAEGIGKVDDIDHLGNRRIRAVGELLANQFRIGLARMERNVRERMSVQDNEVLTPQ
                     QIINIRPVTAAVKEFFGSSQLSQFMDQHNPLSELSHKRRLSALGPGGLTRDRAGYEVR
                     DVHYTHYGRMCPIETPEGPNIGLINNLSSFGHLNKYGFIQTPYRKVDRSTGAVTNEIV
                     WLTADEEDEFTVAQANSKLNEDGTFAEEIVMGRHQGNNQEFPSSIVDFVDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLIDPKAPYVGTGMEYQAAHDSGAAVIAKH
                     DGRVIFSDAEKVEVRREDGSLDVYHVQKFRRSNSGTAYNQRTLVKVGDLVEKGDFIAD
                     GPSMENGEMALGQNPVVAYMTWEGYNFEDAVIMSERLVKEDVYTSVHLEEFESETRDT
                     KLGPEEITREIPNVGEDSLRDLDEMGIIRIGAEVKEGDILVGKVTPKGEKDLSAEERL
                     LHAIFGDKSREVRDTSLRVPHGGDGVVRDVKIFTRANGDELQSGVNMLVRVYIAQKRK
                     IKVGDKMAGRHGNKGVVSRIVPVEDMPYLPDGTPVDIMLNPLGVPSRMNIGQVMELHL
                     GMAARNLGIHIATPVFDGASSEDLWETVQEAGMDSDAKTVLYDGRTGEPFDNRVSVGV
                     MYMIKLHHMVDDKLHARSVGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGASNVL
                     QEILTYKSDDVTGRLKAYEAITKGKPIPKPGVPESFRVLVKELQSLGLDMRVLDEDDN
                     EVELRDLDEGEDDDVMHVDDLEKARVKQETEEKQAEQVSEVVQED"
     gene            complement(15196..17493)
                     /gene="pbp1b"
                     /locus_tag="ACUBV3_000859"
     CDS             complement(15196..17493)
                     /gene="pbp1b"
                     /locus_tag="ACUBV3_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein PBP1B"
                     /protein_id="HHU3847376.1"
                     /translation="MFKGNKKLNSSKLGDYTPLEFGSIFLRIVKLLSDFIYVIILLFV
                     MLGVGLAVGYLASQVDSVKVPSKNSLVTQVNTLTRVSRLTYSDKSQISEIDTDLQRTP
                     VAKDAISDNIKKAIIATEDENFNDHKGVVPKAVLRAAAGSVLGFGESSGGSTLTQQLL
                     KQQILGDDPSFKRKSKEIIYALALERYMDKDSILSDYLNVSPFGRNNKGQNIAGIEEA
                     AQGIFGVSAKDLTIPQAAFLAGLPQSPIVYSPYTADAQLKSDKDLSFGIKRQKNVLYN
                     MYRTRALTKDEYKSYKDYDIKKDFIKPAVATTNHHDYLYYSALSEAQKVMYNYLIKKD
                     NVSEHDLKNDETRATYRHRAIEEIQQGGYTIKTTINKSVYQAMQDAAAQYGGLLDDGT
                     GKVQMGNVLTDNSSGAIIGFIGGRNYSENQNNHAFDTARSPGSSIKPILPYGIAIDQG
                     MLGSGSVLSNYPTTYSSGEKIMHADEEGTAMVNLQESLDISWNIPAFWTYKMLRDRGV
                     DVKNYMEKLDYPIENFGIESLPLGGGIDTSVAQQTNLYQMIANGGVYHKQYMIESIED
                     SNGKVIYNHESKPVRVFSKATATILQQLLHGPINSGKTTTFKNRLQGLNSGLAGVDWI
                     GKTGTTNSTSDVWLMLSTPKVTLGGWAGHDNNASLAKLTGYNNNANYMAHLVNAINNA
                     DGNTFGKSERFRLDDSVIKAKVLKSTGLQPGVVTVNGRRITVGGESTTSYWAKNGPGT
                     MTYRFAIGGTDSDYQKAWSTLGGKR"
     gene            17604..18863
                     /gene="tyrS"
                     /locus_tag="ACUBV3_000860"
     CDS             17604..18863
                     /gene="tyrS"
                     /locus_tag="ACUBV3_000860"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HHU3847377.1"
                     /translation="MNIFDELKERGLVFQTTDEDALRKALEEGSVSYYTGYDPTADSL
                     HLGHLVAILTSRRLQLAGHKPYALVGGATGLIGDPSFKDVERSLQTKETVVSWGNKIR
                     GQLSNFLEFETGDNKAVLVNNYDWFSNISFIDFLRDVGKYFTVNYMMSKESVKKRIET
                     GISYTEFAYQIMQGYDFYELNKNYNVTLQIGGSDQWGNMTAGTELIRRKSNGVSHVMT
                     VPLITDSTGKKFGKSEGNAVWLDADKTSPYEMYQFWLNVMDADAVRFLKIFTFLSLKE
                     IEDIRIQFEEAPHQRLAQKTLAREVVTLVHGEKAYKEAVNITEQLFAGNIKGLSVKEL
                     KQGLRGVPNYHVQTEDNLNIIDLLVTSGVVNSKRQAREDVSNGAIYINGDRIQDLEYT
                     ISENDKLENEITVIRRGKKKYFVLNFK"
BASE COUNT         5925 a         3846 c         3075 g         6066 t
ORIGIN      
        1 agagagtatc aatagcaaga actttgcata ggaaaaagtt ataccaaacc agacattaat
       61 cagacacaat accactaaaa caaggacaga taaaaggaaa aggagtcgac aagacatatt
      121 agcaataaat tgattacgct catcaaaaac tttggtatag taatgttgaa atcgcttttc
      181 actaaatgtt atcaccaaaa aataaaaact tcctgcaaat aaggcaatac ctgaaccaaa
      241 ggaaaaacct ttagtaaaat tagattcaaa ggtatgtaga aatgatgata ctatcataat
      301 caaactacat acactaaaaa caaaagacaa aactctcata tatactttgt aatctttcgg
      361 agatacttta ctattgggtg acaataatcc ttttattaac atagaatact actttctaaa
      421 acttcaattt aatcatcagc tattatatcg ttttcttcaa tgaagcatag cgataaatag
      481 caaaaataga catcgcaata ctaatactaa tagtaatgat aaaaacatat tgctggtaaa
      541 cgtttggtgg acaaaaatca atagtccaca tccttgacat attacagatt aacttttaac
      601 cttttgtctt ttgtggactg tatttttcca ttaaaatcct atcctctttc actttctgta
      661 taaataaaaa cttcctcaat ttgtttatca aaataacgcg ctatcttaaa tgctagctcc
      721 aaagatgccg tgtatttctc tttttctaga gaaataatag tttgcctagt aactccaagt
      781 gcaactgcaa gttctgcttg acttaatttc ctagattttc gtaacttttg tagagaattt
      841 ttcatatctt acaccttttt gtatagttaa ctttacattt atagtatagt taactataca
      901 ttttttgtca agtacatttt acatacctgt tcaaaaaaca aaaaagttga tagttaaact
      961 atcaactttt ctggtcttat caattttttg ataacttatt tagctttaag gcgctcaacg
     1021 tcacgcgcaa tcattaattc ttcatcagta ggaataacta aaaccttcac ttttgaatct
     1081 ggttttgtga tatctccaaa ataaccaaaa acattttttt ctggatcaag ttcaatgcca
     1141 aaccatgata atcctgcgat aacatcttga cgcattaatg gcgcattttc tcccatacct
     1201 gcagtaaaaa taatagcatc cgcgccattt aatactgcca agtattgtcc aataaatttc
     1261 ttaatacggt caataaagac attataggct aacactgcat ttggatcttt actttgaaga
     1321 cctgcttcaa tatctcgcat atcacttgaa gttcctgata ctccaagcaa tcctgattgt
     1381 ttgttaagca tattaacaac agctgctgca tcttccaatt ctgaatcatt agctacaaga
     1441 taagggataa tcgcaggatc aatgtctcct gaacgtgttc ccatcattgg tcctgcaagt
     1501 ggtgtgaatc ccattgatgt atcaatagac tgtccatgat aatttgctgt aatagaaaca
     1561 ccattaccca catgtgctgt gattaatttt aattcttcta aaggacgtcc taattgctta
     1621 gctgcttctt gtgcaacgta ttggtggctt gtcccgtgtg caccgtattt acgtacttta
     1681 taatcagtat agtacttttg tgggattgga tataagtagg tatgaggttg cattgttgta
     1741 tggaaggctg tatcaaatac acatacactc gtgatatctg gtaagatttc tctaaacgca
     1801 cgaatccctg ctgcagctgc tgggttgtgg agtggagcta aagctgataa ttcttcaacc
     1861 tgttctacaa ctttatcatc tactaaagca gactctttaa aatattcacc accagcaacg
     1921 acacgatgac caacacctgt aatttcatta aagtctttga taataccatg ttttgttaga
     1981 tcttctaaaa gaattttaac agcttgggta tgatctacga tatctaaaat ttgttcatct
     2041 tttttatcgt caaattttac agtcgaaata gaatctttca aaccaatacg ttcgataatc
     2101 cctttggcaa ctactttctc ctctggcatt tcatataatt gccatttcaa acttgaactt
     2161 ccagcattaa ttgctattgt ttttgacata atttacctct ctaaagcgtt ttcacaatct
     2221 ctattctatc atattttttg agtaatgact attttctctg ctccattttt gaaaattttc
     2281 aatgaagttt gccatattct cacgattttg caaatctgtc agcggatata caaaggtttc
     2341 tggtttttgt tctgcttgct tcttgagaac aaatatagat ttcgcatttt gacgacttcc
     2401 aaaaatagtt tctggtagag ttaaaacggc aatgacatct gcatatcctt ttaaccactc
     2461 cttcagcaaa tcactttgtg gacttgttaa aaggttttcg ggtgctaaaa atatagcgat
     2521 tccatctttt ttcaaatatt taagagattg ctccatcaat agatggtgag cataggtgtg
     2581 ctctttagaa cttgatacag catatcgttt agcaatatca tcattaggat aatagccaat
     2641 tggtaaatca ctaatgatta catcgctttc tttcaaaatt tgtggtctaa cggcatcctc
     2701 ttggataaat tgggcactag aacctataat ttcagcaatg cttgctgata gatcaatcaa
     2761 aagatcatca acttcaatgc ccatataatt taactctttc gagctgttat tgaggagagt
     2821 ctgagctaaa ttcccagttc cacttccaat ttccaagaca tccactgtct cttgactcgt
     2881 taattcttcc aaaagaaata acaagataaa accaatacta tctggcgtaa actgatgatt
     2941 agcttgtaat tgctctgttt gcgcagattt gataaaaatg aactggaaag tgcgacgcca
     3001 ttcttcttga cttaagtcaa gttgacgtaa tttttggtta ttcaccacaa caatatctaa
     3061 atcacaactt gaaccaaggt aataagagtt ctgttcaatt aaggcatcat aaatatgagt
     3121 ttttaattgg ttctcaatcg tttggatatt ttctaaaatc agctcatagg ctgtctcaat
     3181 tttttcaaaa ttcatgattt cctccagaat ttatcataac aaaaaagaca gcatctgaaa
     3241 agagaagcaa acaaaaatcc tgtttttaag caaaagcttg tcttactaat atttcatcac
     3301 tgtatatttt taatgtttat ggcttgcttt attatcatta gacttgagag gtacttggag
     3361 agtgtctgtt ttacgataaa acttatggtt atagtttcct cccttatcca atgtaacctt
     3421 aacttgtaaa tagttccctt cttggaggta tgaagcatat cctcctgtaa aataacattt
     3481 tcctgatatg gagtcacctt ctattgtttt ataagctaac tgcgctgtta aataagcttt
     3541 acttgcttca atttggctat gatactgtct ttctgttgcc aatattcttg ctagataaaa
     3601 ctgtaagacg agtgtaaata cagcagccaa tacaatagct tgcaaaagga tgccagcttt
     3661 aagtttcttc tttaaaatca tagtaaaatg tcctttttaa cccgtcctta aaataaaaaa
     3721 caagttttac catacttttg gtttgactca tttgacaatt gtctaaccca taaaccattg
     3781 gttgataacc tcgaccatca taacctgtct tacggaaatc atctttatta gatttgccaa
     3841 aggttacaat cttatcttgc ttacgtaaat aaagtttgtt ctgtcttaaa tattccaagt
     3901 gagcgccttc gaattctgca tttagttgct gagttaataa cacccattca gactgactgg
     3961 aagaagacat cactactatc tgttgagcca acaattttgt cagtccttga taaactagta
     4021 aagctcctgt gattgtaacc aatgctacca aacattctaa aagcgtaaat gctttaaccc
     4081 ttaacttttg atacgtgcaa aatttcctta ttaccttcaa agagcacaat tttattttta
     4141 ctttcttcaa tgcgaatatt aacaccattt gcttctaata ttttttgatc agtttgcaca
     4201 gccattctag ctaatatcaa tacttcttgc tcatgttgcc ttcttgcaat atgagattgt
     4261 tgacgattga tttcacttat tactaaactt gtaatagtaa ctaaaatagc taatcccact
     4321 aggctttcca gtaaaatata ggctctaatt ttctttctta cgataattac cacttcctat
     4381 atataattga tacgtaacag tttttctata acattcaaat tgaatttttg ccaagcttga
     4441 attccctcca ttagcgtcaa atgcaagtgt gtcacttttg actactttta cagttttagg
     4501 taaataaagt ctctcataag tattttcgag ataattatga gagattgtaa gggtttgttt
     4561 cttctgacca aatgcactca atttctgagt atctctataa agatgttcaa aggatatgaa
     4621 gaaaattgtt tcttcaacct gcctaaaaat attattaaat gatgttgaaa atactaacgt
     4681 cataaatgcc actactgata atacaataag gctctctaaa agtgtaaatg ctttaacctt
     4741 cttatcctta cattttaaca ataaattttt catgataata tctccatatt ttgatacatt
     4801 ggtaacagca ttgctgcata aatcataaca atgataagag ctacaaaaat aaaaataaca
     4861 ggttggatta actgggtcgc tctagctaat tttgtaaaaa aatcctccca cttctcatca
     4921 gcatatatat ctaactctgt tcctaatttc gccttaactt ggccatattc aatcattaaa
     4981 ctaagctcag ttaagaaaaa cggatagtct aatacttttt ggtgaaatgc tttacctgat
     5041 agaaaacctt cttccatgtc atatcctatt tccctaaaaa gtttggattt ctgattttgc
     5101 attactttaa caatttggtc caattcaaca ccttgactta ataaatttcc ccactcacgg
     5161 gcatagtaag cagttaaata aagcttaaca tatgatccaa ctaagggaat tgttgttaaa
     5221 aaacaagcta ctttaatacg cgacaatcgc ttttgaacaa tataaaatat taaactaaaa
     5281 ataagtacaa ctgctaaaag taataagaaa atattcggca catttgtaat caggctagtt
     5341 gcaaaattat tttctcctaa ttggggcatt aaataatgcc taagaccaat cataattagc
     5401 accagaaaag tcaataatat caatgggtaa gtagctactt ctattacttt ttttctaact
     5461 aacaaaagat tagctaaata agactcaatc tttagtaggc tccttgaaat gttaccatgc
     5521 aaatcagcta atgcaacctg tgtgataaca gtgtcagaaa aacctaactc tcctaacatt
     5581 tttgataaat cttttccctc taataatgcc ttattcatcc tatctgtata ataatcagac
     5641 aataacttac tcctctttaa gaaggtgacc atatcagtta aagaaaaacc actagcaaaa
     5701 agattattaa aaagttggac tactttccgt tgtttcttga gggataattt tttcgacttg
     5761 tgcctgtttc ttactgatat gtccttcttc agccaagata tccacttgtc tattccactt
     5821 gtctgatgag tgttttttaa agttacctgt ctcaaagtca attaggcttc ctcctccaat
     5881 taaacgttga tatgctatta attttagact attttctaac tcttgatagt taacccctaa
     5941 ttctataagc ctatcataga ctccggaaat acttttagca tgaatagtag aaaaaaccat
     6001 cactcccgtt aaacttgcac gaataacagc acgggccgtc gcttgatctc taatctctcc
     6061 gataattaaa atatctggac gatgccgtaa agacagtttg attaaagcat cataagtcat
     6121 tccaatatcc tcattcaatt ggagttgtaa catcttgtca ttcttgattt ctaccggatc
     6181 ttcaatcgtg ataatttgct tatttttaaa tacttctgaa gctaattgat acatgagagt
     6241 tgttttacca ctccccacag ggccggaaaa aagatatagc cctcttgtac ccagtacttc
     6301 cttcatttgc tttatattat caaaccaata ttttaagtcc tgatgacctg aatacaaaat
     6361 acgaataact aaagattctt gaccacgata atctcccaca ctcgatagtc gtaatgaaac
     6421 cagtcttccc tctgacagtt catagtcaca agaacctaat tgacttcgtc ttttttctcc
     6481 aacgttcatg cctgccacaa atttaaagtg actaataaga ctagccatcc tattaaactc
     6541 aaaaacatca ataaaccgcc tttcatcatc aatacgcata tagagttcat aacaatcacc
     6601 tttgggaatg atataaatat cttgagcatt tacttctact gcctgatgaa tgacttgctt
     6661 tgctaatgat tgaaccatcc cgtacctcct taaccatata ttcgaaaata aaataaaaaa
     6721 atctcaccaa ttggtgagat tttttcaatg atttcttgag aggtagtaac ataaatatta
     6781 tgaagcagag gctgaacaaa cctattataa ccccataaaa actatttaac tttttttaaa
     6841 ctacttttaa ttacaaattt aaagaacatg ccgatggcaa ctcagctatc ttattaaata
     6901 cttcaaatcg ctcaatagat tcttccttag gaatttcttg ccattctttc aataacatta
     6961 aggaatgata ctgttggagg tactcatcac attcttcaca ccaacgtttt tcttttatac
     7021 tccaaaacgt tgccaacaaa ctagacttac tttgaaaata aggccacttt tcctgtcccc
     7081 tatcccactc ctcttgaaaa tagagcaaag cttctgataa ggtatcatac tcagcaattt
     7141 cagtaatatc ttcctcccaa ccttcaataa accaccatgg ttcccaatca ccaaacattt
     7201 ttacaacttg atacataata cacttccttt agagagtatt atataaaaaa taagaataaa
     7261 ataaaaaata tctgctcatt ccaaattctt gtaaaaaaag aacctgagtt aaactcaagt
     7321 tctttttgat taatcttctg ttgtaggtgt tgatacttcc gtaacttctg catcaacagg
     7381 agttccttca ataatctcaa cttcattaac agcaagtggt tcaatattac ggtaacgagc
     7441 cataccagta cccgctggaa tgattttacc gataataaca ttttctttaa gaccaagtaa
     7501 gtgatctttc ttaccacgga tagcagcatc tgtaaggaca cgtgttgttt cttggaagga
     7561 tgcagctgat aagaatgaat tagtttcaag ggaagcctta gtaataccca taaggacagg
     7621 acggcttgtc gcagggattc caccagaaat aacaatatct ttgttagcat ctgtaaagtc
     7681 tgaaatatcc ataagtgtac ctggaagaag atctgtatct cctggatcca tgacacgaac
     7741 tttacggagc atttgacgaa ccattacctc aacgtgttta tctccgattt ctaccccttg
     7801 gctacggtaa actttttgta cttcagcaag taggtacgtt tcaacagata aagtatcacg
     7861 aacttcaagg agacgttttg gttggataga tccttcagtt aaggccgcac cacgagctac
     7921 ttcatcacca acttcaactt tcatacgtgc tgtaaatgga acaacgtatt ctccctcacc
     7981 tgtctgacct ttaacgaata ctttcttagt acgtgttgaa gagtcttctt caattgcaac
     8041 gacttcaccc ttaacttcag taataactgc ctcaccttta gggttacgtg cttcaaagat
     8101 ttcttggata cgtgggagac cttgtgtaat atcagtattt gaagcaacac caccggtatg
     8161 gaaggtacgc attgtaagct gtgtccctgg ttcaccgata gattgagctg caatagtacc
     8221 aactgcttca ccaacctcaa cagcatcacc tgttgccaag ttaataccgt aacagtgacg
     8281 acaaacacca tggcgagtgt tacatgtaaa tactgatcgg atagttactt cctcaacgcc
     8341 cgctttaaca actttagcag ccatatcttc agtaattaag gtatctgcac caacgagaat
     8401 ttcacctgtc tcaggatgtt tgattgactt cttagtataa cgtcctatca aacgctcttc
     8461 aagtgtctca gtaacctctt taccatctgt aattgctgtg attgtcaaac cgcggtctgt
     8521 tccacagtca tcttcacgaa taataacatc ttgggcaacg tcaaccaaac gacgagtaag
     8581 gtaacctgag tcggctgtct taagggccgt atcagtcata cctttacgcg caccgtgagt
     8641 tgagaagaac atttccaaaa cactaagacc ttcacggaag tttgataaga ttggcaattc
     8701 catgatacgt ccattaggag cagccatcaa accacgcata ccggcaagtt gtgagaagtt
     8761 tgagatgtta ccacgagctc ctgagtccat catcataacg atagggttct ttggatcttg
     8821 tgtctcaata agacgttttt caagtgcttc tttagcttca cgccatgttg ttgtaacagc
     8881 aacataacga tcttcttcag tcatcaaacc tcgacggaag gctttattga tatcttcaac
     8941 tctgtggtga gctgcatcaa taatctcagc tttattgtca ataactggga tatcagcaat
     9001 acccacagtc aaaccagcaa gtgttgaatg ataatatcca aggtccttca aacggtcaag
     9061 gaaagcagaa gtttctgttg tacggaaacg tttaaaagtt tctgcgataa tattaccaag
     9121 atttttcttc ttaaagggaa tattaatctc taagttatca ataactgctt gaatatcctg
     9181 acctggctct aagaagtatt tatctggagt tttttctgtc aagtttgcat tattaggttc
     9241 aattaagtac ggtaaatctt caggcatgat atcgttgaag agaatcttac caactgttgt
     9301 aaccatgatt ttatgttttt gctcttctgt ccaaggctta ttcggcattg aatcaacagc
     9361 gataccaaca cgtgtatgta aatgaacata accattttga tatgccataa ctgcttcgtc
     9421 atgatctttg aaaatcatgc cttcaccttc acgaccagca tcttccatag tcaggtagta
     9481 atttcctaaa accatgtcct gagatggtgt tacgactggc ttaccatctt tagggttaag
     9541 gatgtgctct gcagcaagca tcaaaagacg agcttctgct tgggcttctt cagacaatgg
     9601 aacgtggatg gccatttggt ctccatcaaa gtcagcattg taggcctcac atacaagtgg
     9661 gtgaagacga agcgccttac catcaataag gacaggttca aacgcttgga tacctaatct
     9721 gtgaagagta ggtgcacggt ttagaagtac tgggtgctct ttaataactt cttctaagat
     9781 gtcccaaata cgctcgtccc cacgttcaac catacgttta gctgctttaa cgttccctgc
     9841 gaggtcacga gcaacgattt cacgcattac aaaaggtttg aagagctcaa tagccatttc
     9901 acgtggtaca ccacattggt acattttaag tgttggacca acagcaataa cggaacgtcc
     9961 agagaagtca acacgtttac caagtaagtt ttggcggaaa cgaccttgtt tacctttaag
    10021 catatggctt aatgatttca aaggacgact acctggtccg gtaattggac gaccacgacg
    10081 accattgtcg ataagcgcat caacagcttc ttgtaacata cgtttttcat tttgaacgat
    10141 gatgccagga gcatttaatt ccaacaaacg tgccaaacga ttgttacggt taataacacg
    10201 gcggtaaagg tcattcaagt ctgatgccgc aaaacgacca ccatctaatt gaaccatcgg
    10261 acgaagatcc ggtgggataa ccggtaaaat gttaagaacc atccattctg gtttatttcc
    10321 agatttttta aaggcatcta acacatccag acgacgaact gctttcacac gtttttgacc
    10381 tgtcgctgat ttcaactcct ctttaagaac tgcgatttct gcatctaaat caacgcgttt
    10441 aagaagatct tgaatggctt cagcacccat tttagcaaca aacgaaccgt aaccgtactc
    10501 ttgaagtttt tcacggtatt cacgctcagt aaggagtgac ttcggttcta atggtgtgtc
    10561 cattggatcg ataacaacat aagccgcaaa ataaataact tcttcaaggg cacggggact
    10621 catatccaac gtgagtccca tacgcgatgg gatacctttg aaataccaga tatgagatac
    10681 tggtgctttt aactcgatat gtcccatacg ttcgcgacga acttttgcac gagttacttc
    10741 aacgccacaa cggtcacaaa tgattccttt gtaacgaata cgtttgtatt ttccacacgc
    10801 acattcccaa tcttttgttg gaccaaaaat aacttcatca aaaagtcctt cacgttctgg
    10861 tttcaatgtg cggtagttga ttgtttcagg ttttttaact tcaccataag accatgaacg
    10921 gaccttactt ggtgatgcta atgtgatttg catactttta aaacgattta cgtcaaccac
    10981 tagttttacc tttcttcgat ttatcatcat actagcctaa ctaggatttc aataaatcct
    11041 aggactagca ttgcatatca attctatatt cgctcctagt tataaactag ataaatttaa
    11101 tcttcttgaa cgacttcaga tacttgttca gcttgttttt cttcagtttc ttgtttcact
    11161 cgcgcttttt caaggtcatc aacgtgcatc acatcatcat cttcaccttc atcaagatca
    11221 cggagttcaa cttcgttgtc atcttcatca aggacacgca tatcaagacc aagtgattgc
    11281 aattctttta caagaacacg gaatgattct ggaacacctg gttttggaat tggcttacct
    11341 tttgtgatag cttcataagc tttaagacgt cctgtaacat catcagattt gtatgttaaa
    11401 atttcttgaa gaacatttga agcaccataa gcttcaagcg cccaaacctc catctcacca
    11461 aaacgttgtc caccaaattg agctttacct ccaagtggtt gttgagtaac aagtgagtat
    11521 ggtcctactg aacgggcatg aagtttatca tcaaccatgt ggtgaagttt gatcatgtac
    11581 atgacaccaa ctgacacacg gttatcgaat ggttcaccag tacgaccatc ataaagaaca
    11641 gtcttagcat ctgaatccat acctgcttct tgaacagttt cccaaaggtc ttctgaagaa
    11701 gccccatcga agactggcgt tgcaatatga atacctaaat tacgagcagc catacctaaa
    11761 tgaagctcca taacttgtcc gatattcata cgtgatggca ccccaagtgg gttcaacatg
    11821 atatcaactg gcgttccatc tggtaaataa ggcatatctt caacaggaac aatacgtgag
    11881 acgacacctt tgtttccgtg acgaccggcc atcttatcac cgactttgat tttacgtttt
    11941 tgagcgatat aaacgcggac aagcatatta acacctgatt gcaattcatc accatttgca
    12001 cgagtaaaga ttttaacgtc acgaactact ccatcgccac cgtgaggtac acgtagcgaa
    12061 gtatcacgaa cttcacgtga tttatcacca aaaatggcat gcaagagacg ttcttcagca
    12121 gataagtcct tttcaccctt aggcgttacc ttaccaacaa gaatgtcacc ttcttttacc
    12181 tcagcaccaa tgcggataat tcccatttca tcgagatcac gtagtgaatc ttcaccaaca
    12241 tttggaattt cacgagtaat ttcttcaggc ccaagctttg tatcacgcgt ttcagattcg
    12301 aattcttcca agtgaacaga tgtatagaca tcttctttca caaggcgctc actcatgata
    12361 acggcatcct cgaagttata accttcccaa gtcatataag caacaactgg gttttgtcca
    12421 agagccattt caccattttc catagatggt ccgtcggcga taaaatcacc tttttcaacg
    12481 agatcaccaa ctttaactaa ggtacgttgg ttataagcag tacctgagtt tgaacggcgg
    12541 aatttttgaa catgataaac atcaagagaa ccatcttcgc gacgcacttc aactttttca
    12601 gcatctgaaa aaataacacg accgtcatgt ttagcaatca cagctgcacc tgaatcgtgg
    12661 gctgcttgat actccatacc agtaccaaca tatggtgctt ttggatcaat caatggcaca
    12721 gcctgacgtt gcatgttggc acccatcaag gcacggttag agtcatcatt ttccaagaaa
    12781 ggaatacatg ctgttgcgac agcaactact tgcttaggtg acacgtcaac aaagtcaaca
    12841 atgcttgaag gaaactcttg gttattacct tgatgacgac ccataacgat ttcttctgca
    12901 aatgtaccgt cttcgttaag cttagagttg gcttgtgcaa ctgtaaattc atcttcttca
    12961 tcagcagtca accaaacaat ttcgttcgtt accgcaccag tagaacgatc tactttacga
    13021 tatggtgttt gaataaatcc atatttattg aggtgtccaa atgaagataa gttattaatc
    13081 aaaccaatgt ttggaccttc aggtgtttca atcggacaca tgcgcccata gtgggtatag
    13141 tgcacatcac gaacttcata tccagcacgg tcacgtgtca aaccaccagg tcctaaggca
    13201 gagagacggc gtttgtgcga caattctgat agagggttgt gttggtccat gaattgtgac
    13261 agctgtgatg aaccaaagaa ttctttaaca gcggcagtca ctggacgaat gttaatgatt
    13321 tgttgaggtg tcaagacttc attgtcttgt acagacatac gttcgcggac attgcgttcc
    13381 atacgagcaa gtccaatacg gaattggttc gcaagcaatt caccaactgc acgaatacga
    13441 cggttaccta aatggtcaat gtcatctacc ttaccgatac cctctgcaag gtttaagaag
    13501 tatgacatct ctgctaaaat atctgcagga gttaaggcac gaactttatc ttcaggattt
    13561 gagtttccaa caatagtaac aacacggtct ggatcagttg gagcaacaac tttgaatttt
    13621 tgaagaataa ctggttcagt aactacagca taatcatttg gtgtgtagac aaacttgttt
    13681 aagtctccat cgatatgctc tgcaattgaa tcaatgacat ctcgtgtcat aacagtgcca
    13741 gcttcaacaa gaatctctcc tgtttcacca tcaactaaat tttcagcgat tgtttggttc
    13801 aataggcgtg ttttaagatt cagtttttta ttaatcttat aacgaccaac agccgctaag
    13861 tcataacgac gtggatcaaa gaaacgtgca actaaaagac tacgtgagct gtctgcagtt
    13921 tttggttcac ctggacgaag acgttcatag atttctttaa gtgcttcatc tgtacgagaa
    13981 tcacttggat ttttatgaat gtctttttca atagtattgc gaacaagttc actgtcacca
    14041 aagatgtcaa caatttcatc gtctccagag aaaccaagtg cacggactag tgttgtgaat
    14101 ggaattttac gtgtgcggtc gatacgtgta taagcaatat cttttgcgtc tgtctcaagt
    14161 tccaaccaag ctccacggtt agggataaca gttgacccat aaccaacttt tccattttta
    14221 tctaccttat cattaaagta aacaccagga gaacggacta attgtgaaac aatgatacgt
    14281 tcaccaccat tgatgatgaa agtacccatt tcagtcataa ttggaaaatc accaaagaaa
    14341 acttcttgag ttttaatttc accagtttct ttattaacta aacggaaagt aacaaaaata
    14401 ggtgctgagt agctagcatc atggatacga gcttcttcca aagtatattt aggttctttt
    14461 aattcataac caacaaattc aagatccata gtatctgtaa agtttgaaat tggaagtaca
    14521 tcctcaaata cttctttcaa gccagcatca agaaaatctt ggaatgaatc agtttgaatt
    14581 tcaattaagt ttggtaaatc aagaacttcc ttaattcttg aaaagctacg acgtgtacgg
    14641 tgttttccgt actgaacttc atgtcctgcc aagtttttag ctccttttta aagatagagt
    14701 gactaagttt ttatctcata aaacttagtg agtttatttc atttgctcgc ctaacaagga
    14761 caagcttata gatgtcgttt gcggtaattt tcagaaagtt taaaaatgta ttacaataac
    14821 cttttttctt aaaattaggc acaaaaagag cagctaaatc tcactttatc aagttgattt
    14881 aactgctgta tatctaggtc ggacaatatt tcaacctaga cagacgacaa atagttacat
    14941 acattataac ttatttttac caataaatca agttctattc tcttgacttc aaggaagctt
    15001 ctaaaatttc aatttgttca tcaacaccct tgagttttag cataacacga tgatttttaa
    15061 acatagcata ctggtgtttt tctaattctt tgaaagaaga ctatacaaga attaaatcat
    15121 attatcttgg aaaaaataaa aggagaacag atcactctat tctcctcaaa taaaactatc
    15181 agtatcgtaa acctgttatc gttttccacc caaagtagac caagcttttt gataatcact
    15241 atcagttccc ccaatagcaa aacgataggt catcgttcca ggcccatttt ttgcccaata
    15301 acttgttgtg ctttcacctc caacagtgat tcgacgaccg tttactgtta ctacacctgg
    15361 ctgtaaacct gtagacttaa gaactttagc ttttataacg ctatcatcaa gtctaaacct
    15421 ttctgactta ccaaaagtat tgccatctgc attattaata gcattaacca agtgagccat
    15481 ataattagca ttattattgt aacctgtcaa tttcgctaaa gaggcattat tatcatgtcc
    15541 tgcccatcca cctaaagtca ctttaggtgt agaaagcatc aaccaaacat cagaagttga
    15601 gttagtagta cccgtttttc caatccaatc taccccagct aaaccactat ttaaaccttg
    15661 taagcgattt ttaaaggttg tagtctttcc ggagttaatt ggtccatgta atagctgctg
    15721 taagatagta gctgttgctt tcgaaaagac acggacaggt ttactttcgt gattataaat
    15781 cactttccca ttactatcct ctatactttc tatcatatac tgcttatgat aaacaccacc
    15841 atttgcaatc atttgataaa ggtttgtttg ctgagcaact gacgtatcaa taccaccacc
    15901 aagtggtaaa ctttctatac caaagttttc aattggatag tctaatttct ccatatagtt
    15961 ttttacatca acgcctcgat ctcgtagcat cttgtaagtc cagaaagctg gaatgttcca
    16021 agaaatgtcc aatgactctt gaaggttaac catagcagta ccttcttcat ccgcatgcat
    16081 aatcttttcg ccacttgaat atgtcgttgg ataatttgaa agaacactac cgctgcctaa
    16141 catcccttga tcaatagcaa ttccgtaagg taatatcggt ttaatacttg accctggaga
    16201 gcgtgccgta tcaaaagcat ggttattttg attctcacta tagttacgac caccaataaa
    16261 tcctataata gcaccactag aattgtccgt tagtacattt cccatttgaa ccttgccagt
    16321 gccatcatct aacaaccctc cgtattgagc tgcagcatcc tgcatggctt gataaacaga
    16381 tttattgata gtagttttaa tagtataccc tccctgttga atctcttcaa tagcgcgatg
    16441 tctgtacgtc gctctagtct cgtcattttt taaatcatgc tctgaaacat tatccttttt
    16501 aatgaggtaa ttgtacatca ctttttgagc ttcagataat gccgagtagt agagataatc
    16561 atgatgattt gttgtcgcca ctgcaggttt gataaaatct tttttgatgt cataatcttt
    16621 gtaggatttg tactcatctt tggttaaagc tctcgttcta tacatattgt ataacacatt
    16681 tttttggcgc tttataccaa aactaagatc tttatctgat ttcaactggg catctgcagt
    16741 ataaggagaa tatacgatag gactttgcgg taaacctgca aggaaagctg cctgaggaat
    16801 tgttaaatct tttgcggaaa ctccaaaaat cccttgtgct gcttcttcaa tacctgcaat
    16861 attctgtcct ttgttattgc gaccaaacgg tgatacattt aaataatctg ataaaatcga
    16921 atccttatcc atgtaacgct ctaatgctag agcataaata atttcttttg atttgcgttt
    16981 aaatgacgga tcatctccca aaatttgctg ctttaataat tgttgtgtca gcgttgaacc
    17041 accgctactc tctccaaacc ccagaacaga tcctgctgca gcacgtagta cggctttggg
    17101 aactacacct ttatggtcat tgaaattttc gtcctctgtt gcaatgatag cctttttaat
    17161 attatcagat atagcatcct ttgccactgg tgtacgttgt aaatcagtgt caatctctga
    17221 aatttgtgat ttatcagaat aagttaacct agacacccta gtaagtgtat taacttgtgt
    17281 cactaaacta tttttactag gtactttaac agagtcaact tgactagcaa gataccctac
    17341 tgcaagtcct actccgagca taacaaatag taaaataata acatagatga aatctgataa
    17401 aagctttaca attcttaaaa aaatagaacc aaattcaagt ggtgtgtaat cacctaattt
    17461 agaactattc aacttcttat tacctttaaa catgaataat acctcatcct acattatatc
    17521 agatttaata taacttgcat atcctaaaga gttttgatat agttgacatt gatacacaat
    17581 aagttaataa ttaggagaaa aagatgaata tttttgatga actcaaagaa cgtggactcg
    17641 tttttcagac aactgacgaa gatgctttac gcaaagctct tgaagaaggt tctgtctctt
    17701 actatactgg ctacgatcct acggctgata gccttcatct tggacattta gttgctattt
    17761 taacctctcg tcgcttacaa cttgctggtc acaagcctta tgcacttgtg ggaggcgcaa
    17821 ctggacttat tggggatcct tcatttaaag atgttgagcg cagtttacaa acaaaggaaa
    17881 ctgtagttag ttggggtaat aaaatccgcg gtcaattatc taattttcta gagtttgaaa
    17941 caggtgataa caaagctgta ttggtaaata attatgattg gttcagcaat attagtttca
    18001 ttgatttctt acgagatgtt ggtaaatatt ttactgtcaa ttatatgatg agtaaagaat
    18061 ctgttaaaaa acgtatcgaa acagggattt cctatactga atttgcttat caaattatgc
    18121 aaggatatga cttttacgaa ttaaataaga actataatgt cactttgcaa attggtggtt
    18181 ctgaccaatg gggcaacatg actgctggaa ccgaacttat tagacgaaaa tcaaatggtg
    18241 tatctcatgt tatgactgtt ccattgataa cagattctac aggaaaaaaa ttcggaaaat
    18301 ctgaaggaaa tgctgtttgg cttgatgctg acaaaacctc tccttatgaa atgtaccaat
    18361 tttggctcaa tgttatggat gctgatgccg ttcgcttcct taagattttt acattcctaa
    18421 gccttaaaga aatagaagat atacgtattc aattcgagga agcacctcac caacgcttag
    18481 cacaaaaaac actagcacgt gaagttgtaa ctcttgttca tggtgaaaaa gcttataagg
    18541 aggccgttaa tatcacggaa cagctatttg ctggcaatat aaaaggattg tctgttaaag
    18601 agctcaaaca aggtttacgt ggagtaccaa attatcacgt tcagacggaa gataacctca
    18661 atatcataga ccttctagtc acttcaggag ttgtcaattc taaacgtcaa gctcgagaag
    18721 atgtctccaa tggagctatt tatattaatg gagatcgtat tcaagattta gaatatacta
    18781 tctcagaaaa tgataagctt gaaaatgaaa ttacagtcat tcgtcgtgga aagaaaaaat
    18841 actttgtatt aaactttaaa tagacttgaa gtgtaatcga tactctgata ataatatttc
    18901 aagacaataa aa
//
LOCUS       DBJYSH010000032        18904 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.032, whole genome shotgun sequence.
ACCESSION   DBJYSH010000032 DBJYSH010000000
VERSION     DBJYSH010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 18904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18904
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.032"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            103..1257
                     /gene="mutY"
                     /locus_tag="ACUBV3_000861"
     CDS             103..1257
                     /gene="mutY"
                     /locus_tag="ACUBV3_000861"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HHU3847378.1"
                     /translation="MIDLKDYGIEMWPEDRIASFRRTLLGWYDQEKRDLPWRRTTNPY
                     YIWVSEIMLQQTQVNTVIPYYKRFLEWFPQIKDLADAPEEQLLKAWEGLGYYSRVRNM
                     QKAAQQVMVDFGGIFPHTYDDIASLKGIGPYTAGAIASISFNLPEPAVDGNVMRVMAR
                     LFEVNYDIGDPKNRKIFQAIMEILIDPDRPGDFNQALMDLGTDIESAKTPRPDESPIR
                     FFNAAYLNGTYSKYPIKNPKKKPKPMRIQAFVIRNQNGQYLLEKNTKGRLLGGFWSFP
                     IIETSPLSQQLDLFDDNQSNPIIWQTQNETFQREYQLKPQWTDNHFPNIKHTFSHQKW
                     TIELIEGVVKATDLPNAPHLKWAAIEDFSLYPFATPQKKMLETYLKQKNA"
     gene            1434..2027
                     /locus_tag="ACUBV3_000862"
     CDS             1434..2027
                     /locus_tag="ACUBV3_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HHU3847379.1"
                     /translation="MEIGQQIIRYRKQQALSQEELAEKVYVSRQSISNWENDKTYPDI
                     YSLLLLSQIFQVSLDQLIKGDIEKMKYTITQVDKKNFERDTKVMVTLMILLMISSYPL
                     VYFLEWLGLGIFVLLSIITMTYANRVERFKKKYDVQTYKEILAVSSGKLLDEIEKREE
                     RAKLPYQKPLIVTVFFLITVATFFASRFIFTWLFHLS"
     gene            complement(2073..2405)
                     /gene="trxA"
                     /locus_tag="ACUBV3_000863"
     CDS             complement(2073..2405)
                     /gene="trxA"
                     /locus_tag="ACUBV3_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001162959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HHU3847380.1"
                     /translation="MKWRKKMALEVTDATFVEETKEGLVLIDFWATWCGPCRMQAPIL
                     EQLSQEIDEDELKILKMDVDENPETARQFGIMSIPTLMFKKDGEVVKQVAGVHTKDQL
                     KAIIAELS"
     gene            complement(2468..2968)
                     /locus_tag="ACUBV3_000864"
     CDS             complement(2468..2968)
                     /locus_tag="ACUBV3_000864"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HHU3847381.1"
                     /translation="MESYEQFYAKLSQPFRKSPQLIILLNFLLKIVTGMMYILYPSFL
                     IFTLWQGMTFQLWLRLLIIPAVGFIALSYIRKRLDFPRPYEKWNIKPLIYKDTEGRSM
                     PSRHVFSATMISMCLLRYYVYFGIVCLILSVLLAICRVIAGIHYPKDVIVGYLIGLIL
                     GLCLFI"
     gene            complement(2972..5311)
                     /locus_tag="ACUBV3_000865"
     CDS             complement(2972..5311)
                     /locus_tag="ACUBV3_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0045910 - negative regulation of DNA
                     recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HHU3847382.1"
                     /translation="MNNKILEQLEFNKVKELILPYLKTEQSQEELSELEPMTEAPKIE
                     KSFNEISDMEQIFVEHHSFGIVSLSSISESLKRLELSADLNIQELLVIKKVLQSSSDM
                     IHFYSDLDNVSFQSLDRLFENLEQFPNLQGSFQAINDGGFLEHFASPELERIRRQLTN
                     SERRVRQILQDMLKEKAELLSENLIASRSGRSVLPVKNTYRNRISGVVHDISSSGSTV
                     YIEPRAVVTLNEEITQLRADERHEESRILHAFSDLLRPHVATIRNNAWILGHLDFVRA
                     KYLFMSDNKATIPEISNDSTLALINVRHPLLSNPVANDLHFDQDLTAIVITGPNTGGK
                     TIMLKTLGLAQLMGQSGLPVLADKGSKIAVFNNIFADIGDEQSIEQSLSTFSSHMTHI
                     VSILNEADHNSLVLFDELGAGTDPQEGASLAMAILEHLRLSNIKTMATTHYPELKAYG
                     IETNFVENASMEFDAETLSPTYRFMQGVPGRSNAFEIASRLGLAPFIVKQAKQMTDSD
                     SDVNRIIEQLEAQTLETRRRLDHIKEIEQENLKFNRAVKKLYNEFSHERDKELEKIYQ
                     EAQEIVDMALNESDTILKKLNDKSQLKPHEIIDAKAQIKKLAPQVDLSKNKVLNKAKK
                     IKAARAPRIGDDIIVTSYGQRGTLTSQLKDGRWEAQVGIIKMTLTQDEFTLVRVQEEQ
                     KVKSKQINVVKKADSSGPRARLDLRGKRYEEAMQELDNFIDQALLNNMGQVDIIHGIG
                     TGVIREGVTKYLRRNKHVKHFAYAPQNAGGSGATIVTLG"
     gene            complement(5396..5938)
                     /locus_tag="ACUBV3_000866"
     CDS             complement(5396..5938)
                     /locus_tag="ACUBV3_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HHU3847383.1"
                     /translation="MLSLLLLIIVIWHFYIGYSRGIFLQVFYVLMSMVSLMIASQFYQ
                     ELASQITLWVPYSNPVQGVEVYFFKDISKFQLSHVYYAGVAFVFIYSLSYLVGRLLGV
                     LLHLAPVEHFDSLQNNIISGFLAVLVCLLFMSMCLTILATVPMSFVQEKLWNSLFVRF
                     LINDLPFFSQFLVRTWAVGL"
     gene            complement(5941..6252)
                     /gene="zapA"
                     /locus_tag="ACUBV3_000867"
     CDS             complement(5941..6252)
                     /gene="zapA"
                     /locus_tag="ACUBV3_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HHU3847384.1"
                     /translation="METKINNSQKNRYKFVFGDKPLTLTTDKDNLFMEEIERVATEKY
                     EAIKEKLPNADNETIAILMAINALSVQLSREIDIEKMEDELNKLRSKTISDIKEKVSE
                     D"
     gene            6363..7256
                     /gene="rnhC"
                     /locus_tag="ACUBV3_000868"
     CDS             6363..7256
                     /gene="rnhC"
                     /locus_tag="ACUBV3_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HHU3847385.1"
                     /translation="MNTIVMQADKKLQEKIRTDLAQHHISNNNPYVVFSAKISGTTVL
                     LYTSGKLVFQGSNASHIAQKYGYIEQKESCSSETQDIPIIGTDEVGNGSYFGGLAVVA
                     SFVTPKDHAYLKKLGVGDSKTLTDQKIKQIAPLLEKAIPHKALLLSPQKYNQVVGPNN
                     KHNAVSVKVALHNQAIFLLLQDGFEPEKIVIDAFTSSKNYQNYLKNEKNQFKQTITLE
                     EKAENKYLAVAVSSIIARNLFLENLNKLSDDVGYKLPSGAGHQSDKVASQLLKAYGIS
                     SLEHCAKLHFANTKKAQALLK"
     gene            7272..7865
                     /gene="lepB"
                     /locus_tag="ACUBV3_000869"
     CDS             7272..7865
                     /gene="lepB"
                     /locus_tag="ACUBV3_000869"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HHU3847386.1"
                     /translation="MKEFIKEWGVFILILSLFLLSRIFLWQFVKVDGHSMDPTLADKE
                     QLVVLKQTKINRFDIVVANEEEGGQKKKIVKRVIGMPGDVIKYKNDTLTINNKKTEEP
                     YLKEYTKLFKKDKLQEKYSYNPLFQDLAQSSTAFTTDSNGSSEFTTVVPKGHYYLVGD
                     DRIVSKDSRAVGPFKKSTIVGEVKFRFWPIRRFGTIN"
     gene            7994..10196
                     /gene="recD2"
                     /locus_tag="ACUBV3_000870"
                     /pseudo
     CDS             7994..10196
                     /gene="recD2"
                     /locus_tag="ACUBV3_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019804593.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SF1B family DNA helicase RecD2"
     gene            10528..11010
                     /locus_tag="ACUBV3_000871"
     CDS             10528..11010
                     /locus_tag="ACUBV3_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000255479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3847387.1"
                     /translation="MVSYEKVRRSLRTATITIIVLNSLSLVFRLFTGISVQLAKTEIN
                     KGNTGNLPKEHIEAVLSATTPFMLFVTALIVLVNIAIVIFCIKNLRAIKRNQMVNYLP
                     YYLGFAITVGLVILGFLTTKAPWAIAINIVFQAIFGLLYFHAYQKAQKLNERDLEETN
                     "
     gene            complement(11081..12175)
                     /gene="dinB"
                     /locus_tag="ACUBV3_000872"
     CDS             complement(11081..12175)
                     /gene="dinB"
                     /locus_tag="ACUBV3_000872"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HHU3847388.1"
                     /translation="MLIFPLINDTSRKIIHIDMDAFFASVEERDNPSLKGKPVIIGSD
                     PRKTGGRGVVSTCNYEARKFGVHSAMSSKEAYERCPQAIFISGNYQKYRQVGMEVRDI
                     FKKYTDLVEPMSIDEAYLDVTENKMGIKSAVKLAKMIQYDIWNDVHLTCSAGISYNKF
                     LAKLASDFEKPKGLTLILPDQAQDFLKPLPIEKFHGVGKRSVEKLHALGVYTGEDLLS
                     LSEISLIDMFGRFGYDLYRKARGINASPVKPDRVRKSIGSEKTYGKLLYNEADIKAEI
                     SKNVQRVVASLEKNKKVGKTIVLKVRYADFETLTKRMTLEEYTQDFQIIDQVAKAIFD
                     TLEESVFGIRLLGVTVTTLENEHEAIYLDF"
     gene            12360..14672
                     /gene="pflB"
                     /locus_tag="ACUBV3_000873"
     CDS             12360..14672
                     /gene="pflB"
                     /locus_tag="ACUBV3_000873"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HHU3847389.1"
                     /translation="MATVKTNTDIFEQAWEGFKGVDWKEKASIARFVQANYAPYDGDE
                     SFLAGATERSLHIKKVIEETKAHYEETRFPMDTRVASISELPAGFIDKDSELIFGIQN
                     DELFKLNFMPKGGIRMAETTLKENGYEPDPAVHEIFTKYATTVNDGIFRAYTSNIRRA
                     RHAHTVTGLPDAYSRGRIIGVYARLAVYGADYLMQEKVNDWNALNDIDEESIRLREEI
                     NLQYQALGEVVKLGDLYGVDVRKPAMNTKEAIQWVNIAFMAVCRVINGAATSLGRVPI
                     VLDIFAERDLARGTFTESEIQEFVDDFVLKLRTVKFARTKAYDALYSGDPTFITTSMA
                     GMGADGRHRVTKMDYRFLNTLDNIGNSPEPNLTVLWSDQLPYAFRRYCMSMSHKHSSI
                     QYEGVSTMAKEGYGEMSCISCCVSPLDPENEDKRHNLQYFGARVNVMKALLTGLNGGY
                     DDVHKDYKVFDIDPIRDEVLNFDTVKANFEKSLDWLTDTYVDAMNIIHYMTDKYNYEA
                     VQMAFLPSHVRANMGFGICGFANTVDSLSAIKYATVKPIRDEDGYIYDYETVGDFPRY
                     GEDDDRVDSIAEWLLEAFHGRLAKHKLYKDAEATVSLLTITSNVAYSKQTGNSPVHKG
                     VYLNEDGSVNLSKVEFFSPGANPSNKAKGGWLQNLNSLSKLDFAHANDGISLTTQVSP
                     RALGKTFDEQVDNLVTVLDGYFENGGQHVNLNVMDLKDVYDKIMNGEDVIVRISGYCV
                     NTKYLTPEQKTELTQRVFHEVLSMDDALTN"
     gene            14773..15147
                     /locus_tag="ACUBV3_000874"
     CDS             14773..15147
                     /locus_tag="ACUBV3_000874"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000931877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HHU3847390.1"
                     /translation="MLNHKFLQVLKYEGVVSITSWTELAPHVTNTWNSYLTITDDQRI
                     LAPAAGMTHLENDLNNNSKIIMTLGSREVEGRDGYQGTGFRIEGTAKLLEAGSDFEIV
                     KKKYPFLRKVLEVTPINVIQLL"
     gene            complement(15182..16111)
                     /locus_tag="ACUBV3_000875"
     CDS             complement(15182..16111)
                     /locus_tag="ACUBV3_000875"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HHU3847391.1"
                     /translation="MIKTNTVLELIKEQIKDNLYHGASLAIYENGEWHEHYLGTIDGN
                     EKVKAGLVYDLASVSKVVGVGTLLAKLVYQGTIDIDKPLRYYYPTFHHQTLTVRQLAT
                     HSSGIDPFIPNRDQLNATQLKDAINHIKVLEDKSFKYTDINFLLLGFMLEEVLGDSLD
                     KLFKRYIFTPFQMKETSFGPRVEAVPTVAGINDGIVHDPKAKVLGKHTGSAGLFSTID
                     DLQRFSIHYLKDDFAKPLWNNYSLSKSRSLAWDIDKDWINHTGYTGPFIALNYQKQAA
                     AIFLTNRTFSYDDRPLWIKKRRHVQEAITSYFE"
     gene            complement(16108..16863)
                     /locus_tag="ACUBV3_000876"
     CDS             complement(16108..16863)
                     /locus_tag="ACUBV3_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CppA family protein"
                     /protein_id="HHU3847392.1"
                     /translation="MTLFHSLTFKHPVLRVNNRDLNIAFYQESLGFKLISEENAIAVF
                     SAWQNKEASFIIEESPTYRTRAVNGTKKLAKIIVKSQDAKDIEKLLANGAQTTQVYQG
                     QNGYAYETVSPEGDLFLLHAEDDLSQLVAIERPELEKNDDTTGLSNFVFQSISLNVPD
                     AVKAEAFYDKVFAGKFPINLSFKEAQGQDLQIAPNETWDIEILECCVNEDTNLNDLKS
                     TFESLGLDVYLDSKEKILVISDTSNIEIWISKE"
     gene            16988..17884
                     /locus_tag="ACUBV3_000877"
     CDS             16988..17884
                     /locus_tag="ACUBV3_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HHU3847393.1"
                     /translation="MNETLLLHGIQLILIIAMIITFYQIVRHIRSQKINPFKRFFTGL
                     WIGFVTDALDTLGIGSFATTTTFFKLTKLVEDDRKIPATMTAAHVLPVLLQSLCFIFV
                     VKVEALTLITMAGAAFIGAFVGAKMTKNWHAPTVQRILGTLLITAAIIMLYRMITNPG
                     AGISDSVHGLHGIWLFVGIVFNFIIGVLMTMGLGNYAPELIFFSLMGLSPAVAMPVMM
                     LDAAMIMTASSTQFIKSGRVNWNGFAGLVTGGILGVIVAVLFLTNLDLNSLKTLVVGI
                     VLFTGAMLIRSSFKKTMPKSHH"
     gene            complement(17963..18811)
                     /gene="gla"
                     /locus_tag="ACUBV3_000878"
     CDS             complement(17963..18811)
                     /gene="gla"
                     /locus_tag="ACUBV3_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000570237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaglyceroporin Gla"
                     /protein_id="HHU3847394.1"
                     /translation="MIEITWTVKYITEFIATAFLIILGNGAVANVDLKGTKGNNSGWI
                     IIAIGYGLGVMMPALMFGNVSGNHINPAFTLGLAFSGLFPWAHVGQYILAQILGAMFG
                     QLVVVMVYQPYFVKTENPNHVLGSFSTISALDDGQKSSRKAAYINGFLNEFVGSFVLF
                     FGALALTKNYFGVELVGKLVQAGYDQTTAATRISPYVTGSLAVAHLGIGFLVMTLVAS
                     LGGPTGPALNPARDLGPRIVHRLLPKQILGQAKEDSKWWYAWVPVLAPIVASILAVAL
                     FKLLYL"
BASE COUNT         6226 a         3594 c         3123 g         5961 t
ORIGIN      
        1 agtcaagaaa aaaatattga tgcaacattt ttcttcatca ttaattacga tctagtcaac
       61 atacctcttt gttaaatcct ctttctttga tacaattaat ctatgataga tttaaaagac
      121 tatggaattg agatgtggcc tgaagataga atcgcttctt ttcgtcgcac acttttaggg
      181 tggtatgacc aagaaaaacg tgatctccct tggcgaagaa ctactaatcc atactatatt
      241 tgggtttctg aaatcatgtt gcaacaaaca caagttaata cagtaatccc ttattataaa
      301 cgttttctag agtggtttcc tcaaataaaa gatttggcag acgctcctga agaacaatta
      361 ctaaaggcat gggagggact aggttattat tctcgggtgc gtaatatgca aaaggctgca
      421 cagcaagtga tggttgattt tggtggcata tttcctcaca cttatgatga tattgcttca
      481 ttaaaaggga ttggtcctta tactgcaggt gctatcgcca gcatctcatt caacttacca
      541 gagcctgccg ttgatggcaa tgtgatgcgt gtcatggctc gtttgtttga agttaattat
      601 gacatcggtg accctaaaaa tcgtaaaatt tttcaagcta ttatggaaat actaattgac
      661 cctgacagac ccggtgattt taaccaagca cttatggatc tgggtacaga catcgaatcc
      721 gctaagacac ctagacctga tgaaagccct attcgtttct ttaatgctgc ttatttgaat
      781 ggtacttata gtaaatatcc cataaaaaat cctaagaaaa agcctaaacc aatgcgaatt
      841 caggcttttg tcattcgtaa ccaaaatggc caatatctac tggagaaaaa tactaaaggg
      901 agacttctag ggggtttttg gtcatttcct attatcgaaa cgtcacctct ttctcaacaa
      961 ttagatttat ttgatgacaa tcaatccaac cccataatct ggcaaaccca aaatgaaact
     1021 ttccaaagag aatatcaatt aaagccccaa tggactgata atcactttcc caatattaag
     1081 cacactttta gtcaccagaa gtggacaata gaactcatag agggggtggt caaagcaaca
     1141 gatttaccta atgctcctca cctaaagtgg gcagcaattg aggacttttc tctgtatccc
     1201 tttgcaacgc cacaaaagaa aatgctggag acctacttaa aacagaaaaa tgcttaatcg
     1261 caagtgaact atgacggttc actttttgtt tacaattcct ttacgccgct gtaaagtttt
     1321 cctcagatta tcttttaata ccctataaat agtggtctat ccctaatgtc aaatacattt
     1381 gatagacccg ttagcttatc attcttatac taatattatg aaaggagata tttatggaaa
     1441 ttggtcagca aattattcgc taccgtaaac aacaagcgct ctctcaagaa gaattagccg
     1501 agaaagtcta tgtaagccgt cagagcatat ctaactggga aaatgacaaa acctacccag
     1561 acatctatag cctcttactc ctaagtcaaa tttttcaagt gagcttagac caactaatta
     1621 aaggagacat tgagaaaatg aaatatacca tcactcaggt tgataaaaag aattttgaac
     1681 gagataccaa agtaatggta acattgatga tactattaat gatttcaagt tatccattag
     1741 tctatttctt agagtggcta ggacttggca tttttgtttt attatcgata attactatga
     1801 cttatgctaa tcgtgtggaa cgttttaaga aaaaatatga tgtgcaaact tataaggaaa
     1861 tcctagctgt ttcaagtgga aaattattag acgaaatcga aaaacgggaa gaaagagcaa
     1921 aacttcctta tcagaaaccc cttattgtca ctgtattttt cctcattact gttgccacct
     1981 tttttgcaag tcgttttatt tttacatggt tgttccattt aagttagtag ataataaaat
     2041 aaagctcagc gtgacgctgg gctttatttt atttaactca attcagcaat aatagctttc
     2101 agttggtctt tagtatgaac acctgcaact tgtttaacca cttctccgtc ttttttaaac
     2161 atcaatgttg gaattgacat aataccaaac tgacgggcag tttccggatt ttcatcaaca
     2221 tccattttca aaattttgag ttcatcttca tcgatttctt gagataactg ttctaagatt
     2281 ggtgcttgca tacgacatgg accacaccaa gttgcccaaa aatcaatgag tactaaacct
     2341 tctttagttt cttctacaaa cgtagcatct gttacttcta atgccatctt ttttctccat
     2401 ttcatcttaa tgataagcct attgtacaag aatttagttg tttttgaaac tatttgctac
     2461 gaactcgcta aataaataaa caaagcccta aaatcaagcc aataaggtaa cctacaataa
     2521 catccttagg atagtgaata cctgctatca cacggcaaat tgccaataga accgataata
     2581 ttaaacaaac tattccaaag taaacataat atcgtaacaa acacatacta atcatcgtag
     2641 ctgaaaaaac atgtctgcta ggcattgacc ttccctcagt atccttataa attaaaggtt
     2701 taatattcca tttttcatat ggtcgtggaa aatcaaggcg tttcctaatg taagacaaag
     2761 ctataaagcc aacagcaggg ataattaata gccttaacca taattgaaat gtcatccctt
     2821 gccaaagagt aaatatcaga aaagatggat agagtatgta catcattcct gtgactattt
     2881 ttagtaaaaa atttaaaaga attattaact gtggggattt tctaaaaggt tgagatagtt
     2941 tggcataaaa ttgttcatat gattccataa tttaccctaa cgttacaatt gtggcgccag
     3001 atccccctgc attttgtggg gcataagcaa aatgcttaac gtgcttatta cgacgaagat
     3061 attttgtcac tccctcacgg ataacgcctg taccaatacc atgaatgata tcaacttgtc
     3121 ccatgttgtt aagcaatgct tgatcaataa aattatctaa ctcttgcata gcttcttcgt
     3181 atcttttacc tctaagatca agtcgagctc ttggtccaga actatcagct tttttaacca
     3241 cattaatctg tttacttttg actttctgtt cttcttggac tctaacaagg gtaaattcat
     3301 cttgtgttaa tgtcattttg ataattccca cttgtgcttc ccaacgtcca tcttttaatt
     3361 gactagttaa ggtacctcgc tgtccatagc tagtcactat aatatcatca ccaattctag
     3421 gagcacgagc tgctttgatt tttttagcct tatttaagac tttatttttt gataaatcaa
     3481 cttgaggtgc taattttttt atttgtgcct tagcatctat aatttcgtga ggttttaatt
     3541 ggctcttatc attgagtttt tttaagatag tatcactctc attcaaagcc atatctacaa
     3601 tttcttgagc ttcttgatag attttttcta actctttatc gcgctcatgc gaaaattcat
     3661 tatagagttt cttaaccgca cgattgaatt tgaggttttc ttgttcaatt tctttaatat
     3721 gatccagtct tctacgtgtc tcaagtgtct gtgcctctaa ctgttcaata atacggttaa
     3781 catctgagtc agaatctgtc atctgcttag cttgtttaac aataaatgga gctaaaccaa
     3841 ggcgagaagc aatttcaaat gcatttgatc gtccaggaac tccttgcata aagcgatacg
     3901 tagggctaag cgtttcggca tcaaattcca tgctcgcatt ctctacaaaa tttgtctcaa
     3961 tcccataagc ttttaattct ggatagtgcg tggtcgccat cgttttgata ttacttaacc
     4021 taaggtgttc taaaatagcc atagccaaac tagcaccttc ttgaggatcc gttcctgctc
     4081 ccagttcatc aaagagaact aaactattgt ggtcagcctc gtttaaaata ctaactatgt
     4141 gcgtcatatg actagaaaag gttgatagac tttgttcaat agattgctca tcgccaatat
     4201 ctgcaaagat attgttaaat actgcaattt tactaccttt atccgctaat actggcaaac
     4261 cagactgtcc cattaattgt gctaaaccga gtgtttttag cataatcgtc ttaccaccag
     4321 tattgggacc agtgatgaca attgcagtta aatcttgatc aaaatgtaag tcattagcca
     4381 cagggttact taacagagga tgacgaacat tgattaatgc taacgtgctg tcattagaaa
     4441 tctcaggtat cgtcgcctta ttatcagaca taaaaagata tttagccctt acaaaatcaa
     4501 gatgcccaag aatccatgca ttatttctaa tagtggcgac atggggtctt aacaagtctg
     4561 aaaatgcgtg taaaatacga ctttcttcat gacgttcgtc agctctaagc tgcgttatct
     4621 cttcgtttag tgtaactaca gcacgaggct caatataaac agtacttcct gaagaagaga
     4681 tgtcatgaac cacaccagaa atacgattcc gataagtatt ttttactggt aggacacttc
     4741 gtccactacg actagcgatt agattctctg ataaaagctc tgctttttcc ttaagcatat
     4801 cctgtaaaat ctgacgaacc cgtcgttcac tgtttgttaa ttgacgacgg atacgctcta
     4861 attctggact cgcaaaatgt tctaaaaaac caccatcatt gatagcttga aaagaccctt
     4921 gcagattagg gaattgttcc aaattttcaa acaaacgatc caaagattgg aaagaaacat
     4981 tatccaaatc agaataaaag tgaatcatat ccgaagaact ctgtaaaact tttttgataa
     5041 ccaaaagttc ttgaatatta agatcagctg aaagctctaa gcgttttaaa ctctcagaga
     5101 ttgaacttag gctgactatg ccaaatgagt gatgttcaac aaaaatctgt tccatgtcag
     5161 aaatttcatt aaaacttttt tctattttag gagcctccgt catcggctcc agctctgata
     5221 attcttcttg tgattgttct gtcttgagat aaggtaatat caattcctta actttgttaa
     5281 attctaactg ttctaaaatc ttgttattca tactcttcat tatacctaaa tttatcacaa
     5341 aatgacaacg ttaaaaaggt tggaactctt actccaacct tactaaacat tctattcaaa
     5401 gacctaccgc ccaagtcctt accaaaaact gagagaagaa tggtaagtcg ttaattaaaa
     5461 atctaacaaa cagactattc cataactttt cttgaacaaa tgacataggg actgttgcca
     5521 gaatagtcaa acacatactc ataaataaga gacaaaccag aacggctaaa aaaccactaa
     5581 taatattatt ttgtaagcta tcaaaatgtt caactggtgc taaatgtagt aaaacaccta
     5641 gcaagcgacc tactaagtaa gataaactgt agataaaaac aaaggcaaca cctgcataat
     5701 aaacatgact caattgaaat ttgctaatat ctttaaaaaa gtaaacttct actccttgta
     5761 caggattaga atacggtacc cataacgtaa tctgactagc taattcttgg taaaattgac
     5821 tagcaatcat caaagatacc attgacatta acacgtaaaa gacttgtaaa aaaatacctc
     5881 tactgtagcc aatataaaaa tgccaaataa caataatcag caaaagcaat gatagcatga
     5941 ttaatcctca ctcacttttt ctttaatgtc cgaaatcgtt ttggagcgta acttattgag
     6001 ctcatcttcc attttttcaa tgtcaatttc tcgacttagt tgcactgata aagcattaat
     6061 tgccatgaga atagcaatcg tttcattatc agcattaggt aatttttctt taatagcttc
     6121 atacttttct gtagctacac gctcaatctc ttccataaaa aggttgtctt tatctgtcgt
     6181 caatgtgaga ggcttatctc caaagacaaa tttataacga tttttctgac tattatttat
     6241 ttttgtttcc atactatacc tctgctagca ttataccgta aaatagggct ttcgtaaaat
     6301 tctacccctt atcattactt gtctattccc ttattcctct tttttatgat agaatattgt
     6361 ctatgaacac aatcgtcatg caagcagata aaaaattgca agaaaagata agaaccgatt
     6421 tagctcaaca tcatataagt aataataatc cttatgtggt attttcagct aaaatatcag
     6481 gaactactgt tcttttatat acttctggaa aacttgtctt ccagggttct aacgcttcac
     6541 atatcgccca aaagtatggt tatattgagc aaaaagaaag ttgttcctct gagactcaag
     6601 atattcccat aattggtact gatgaagtgg gaaatggctc ttactttggt ggactggcag
     6661 tggtagctag ttttgtaact cctaaggacc atgcttacct caaaaaatta ggtgttggtg
     6721 attcaaaaac acttactgat caaaaaatta agcaaatcgc acctcttttg gaaaaagcaa
     6781 ttccacacaa agcattattg ttatcgcctc aaaaatataa tcaagtggtt ggtccaaata
     6841 acaaacataa tgctgtctcc gtaaaagttg ctttgcacaa ccaagctatc ttccttcttc
     6901 ttcaagatgg ttttgaacca gaaaaaatcg ttattgatgc ttttactagc tccaaaaact
     6961 atcaaaacta tttgaaaaat gaaaagaatc aatttaaaca aacgattact ctggaagaaa
     7021 aagctgaaaa taaatatcta gctgtagcgg tcagttctat tattgcacgt aatctattct
     7081 tagaaaatct taacaaatta agcgacgacg taggatataa acttccaagt ggagctggtc
     7141 accaatctga taaggtcgct agccaattac tcaaagctta tggtatctct tcgcttgaac
     7201 actgcgcaaa acttcatttt gccaacacca aaaaagcaca agcattatta aaataaatag
     7261 cagggaaaat tatgaaagaa tttattaaag aatggggtgt ctttatcctc atcctctcac
     7321 tttttttact atcgcgtatc tttttatggc aattcgttaa agttgacgga cactccatgg
     7381 atccaacttt agctgacaag gaacagctag tagttctcaa acaaacaaaa atcaatcgat
     7441 tcgatattgt agtggctaac gaagaagaag gcggccaaaa gaaaaaaatt gttaaacgtg
     7501 tcattggtat gccaggtgat gtcatcaaat ataaaaatga caccttaact attaacaata
     7561 aaaaaacaga agaaccttac ctcaaggaat atactaaatt atttaaaaag gataaattac
     7621 aggaaaaata ttcgtataac ccacttttcc aagacctagc acaaagctct accgctttca
     7681 ctactgacag caatggcagc agcgaattta ctactgtcgt gcctaaaggc cactattatc
     7741 ttgttggtga tgaccgaatt gtctctaaag atagtcgtgc cgtcggtccc ttcaaaaaat
     7801 caacaattgt gggagaggtt aaattccgct tctggccaat tcgtcgtttt ggaactatca
     7861 actaaaggaa attaaaaatc cacctgacaa aagttgaaat aacatattat atttaaaata
     7921 gggagtgaca aatccattta aaatagggat ttacaatcac tccctttaac tgtttattaa
     7981 aaaggaaggt acaatggaag tattttttac aggaaccatt gaacgcatta tatttgaaaa
     8041 tgccagcaat ttttttaaaa tattacttct ggaaatcgaa gatacggata gcgattttga
     8101 tgatgtagaa gtaattatta ctggtactat ggccgacgtt atagagggag aagaatatac
     8161 tttttgggga acactaaccc aacatccaaa atacggcgag caactacaaa gtgtacgcta
     8221 tgaaagagcc aagcctactt caggtggcct tgtgaaatac ttttctagcg aacaatttaa
     8281 aggaattggt aaaaagactg cccaacgcat cgtagaactc tatggagata ataccattga
     8341 taaaatcctt gaatcccctg aacagttaag tacaatctca ggactatcaa aaattaatag
     8401 agaagcattc atcgctaaac tgaaactcaa ttatggaaca gagcaagttt tagctaaact
     8461 cgctgaatat gggttaagca atcgtgctgc tattcaaata tttgatcact ataaagaaga
     8521 atcacttgaa gtgataaacg aaaatcctta tcaacttgtt gaggatatcc aaggtatcgg
     8581 ttttaaaata gctgaccaac ttgctgaaca agtcggtatt gaaagtgatt ctcctaaacg
     8641 ctttcgtgct gctattattc atactttagt tgagtcttct atggagcaag gagataccta
     8701 tattgaggct cgtactctct tggaaaaaac cattactctc cttgaagaag ttcgtcaaat
     8761 cgaactagat ccttcaattg ttgctaagga attgacaaat ttaattgcag aagataaagt
     8821 gcaacatatt ggaactaaaa tttttagtaa tactctattt tttgccgaag aaggcatcaa
     8881 aaaaaactta caacgaatac taaatcagcc tcttgataaa caactaagtc acaaagatat
     8941 tgaccgtgaa atcagagata ttcaaaaatc tttaaatatc cactacgata atatccaaga
     9001 aaaagctatt agagaagctt tactaagtaa ggtatttatc ttgactggtg gcccaggaac
     9061 aggtaaaaca acagttataa atggtattat tgaagcctat tccgagttac atcatattga
     9121 tttaaacaaa aatgatattc ctattgtact tgctgctcct acaggtcgtg ctgctagacg
     9181 catgaatgaa cttaccggtt taccgagtgc tactattcat aggcatttgg gattaaacgg
     9241 agatagtgac tatcaaagtc tagatgacta tctagattgt tccttaatta tcattgatga
     9301 attttcaatg gttgacactt ggcttgctaa tcaattattt gacgctttgg actcgcacac
     9361 acaagttatt attgtcggag atagtgatca attaccgtct gtaggtccag gacaagttct
     9421 agcagatctt ttaaatataa atgcacttcc acacattaaa cttgaaaaga tttttagaca
     9481 atcagaagaa tcaactattg tgactttagc taatcaaatg agacagggct ttttgccaga
     9541 ggactttaca gctaaaaaag ccgatcgctc atactttgaa gcatctgcta atatcatccc
     9601 aaatatgatt tctaaaattg ttcaatctgc acttaagagc ggtattgaag ctcatgaaat
     9661 tcaaatttta gctcctatgt atcgcggcca agcaggtatt aataatctta accttatcat
     9721 gcaaaatctc ctcaatccat taaaagataa taaccaattt acttttaatg atataaattt
     9781 ccgaatagga gataaagtgt tacatttagt caacgataca gagcttaatg tttttaatgg
     9841 ggatattggt tacataacgg accttatacc agctaaatat actgagagca agcaagatga
     9901 gatttatatg acttttgatg gtcaagaagt tatctaccaa cgcaaagaat ggcttaaaat
     9961 aacactagcc tatgctatga gtattcataa atcacagggt tctgaatttc aagttgttat
    10021 tctgccaata acgagacaga gtggtcgaat gttacagcga aaccttattt atacagctat
    10081 tacgagatct aaaagtaaac taatactttt aggagaaatt ggtgcatttg atttcgctgt
    10141 taaaaacgag ggggcaaagc gcaatactta tctgatagaa agatttgaaa acaagcaaga
    10201 aatagctaat tctcaaaaaa ttgaagatag ctcaatagat caaaagattg acaacacaat
    10261 aattaatacg tccattccaa aaacagcaac accaattgag cagactaatc tttcaaaaat
    10321 aacttatcgc ctaactgaag aaaattattt aacaatcgat ccaatgattg gtattaatca
    10381 acaagatata agtgcaattt ttgatagtaa ataaattttt tcataaaatc ttgaaaactc
    10441 ttccaaatat gttataatat cattgtatta agaacgtagt tcaaatttaa tacttttatt
    10501 aaggtttatt tagaaaaagg agatattatg gtttcatatg aaaaagtccg ccgctctctg
    10561 cgcacagcta caatcactat cattgtatta aatagtttgt ctttagtatt cagattgttt
    10621 acaggaattt cagtgcagct agccaaaact gaaattaata aagggaacac aggtaatctt
    10681 cctaaagaac atatcgaagc agtcttgagc gccacaacac catttatgct atttgtcact
    10741 gctttaatag tattagtcaa cattgccatt gttattttct gtattaaaaa tttaagagca
    10801 attaaaagaa atcaaatggt taattacctt ccttattatc ttggcttcgc cattactgta
    10861 ggacttgtta ttttgggttt tctaactact aaagcaccat gggcaatagc tataaatatc
    10921 gtcttccaag ctattttcgg tcttctttat ttccatgctt atcaaaaagc acaaaaactc
    10981 aacgaacgtg atcttgaaga gactaactaa aagtaaaagc actagaatct agtgcttttt
    11041 tcttttcaaa gagagttaaa tatagtcatc gtcccaactt ttaaaaatct aaatagatag
    11101 cttcatgttc attttccagg gtcgttactg taacacctaa gagtcgtatg ccaaaaactg
    11161 actcttctaa ggtatcaaat atcgctttag caacttgatc aatgatttga aaatcttgag
    11221 tatactcttc taatgtcata cgtttcgtca atgtttcaaa atccgcatat ctaactttta
    11281 aaacgattgt cttaccaact ttcttatttt tctccagaga agcaacaact ctctgaacgt
    11341 tctttgaaat ctcagctttg atatctgctt cgttatatag taatttccca taggttttct
    11401 cacttcctat tgatttacga actctatcag gttttaccgg ggaagcatta atccctcttg
    11461 ccttacgata aaggtcatac ccgaaacgac caaacatatc aattaaagaa atttcagata
    11521 aggaaaggag gtcctcacca gtataaaccc ctaaagcatg taatttttcg acggagcgtt
    11581 taccaacacc atgaaatttc tctattggta agggcttcaa gaaatcttga gcttgatctg
    11641 gtaggatgag ggtaagtcct tttggtttct caaagtcaga agccaatttt gccaaaaatt
    11701 tattgtagga aatacccgct gaacaggtca aatggacatc attccaaata tcgtattgta
    11761 tcattttagc aagtttaaca gcagatttta ttcccatttt attttcagtt acatctaaat
    11821 aagcttcgtc tatactcatc ggttcaacca aatccgtata ctttttaaat atatctcgta
    11881 cttccattcc tacttggcga tatttttggt aatttcccga aataaagata gcttgagggc
    11941 atcgttcata cgcttctttt gaagacatag ctgaatgaac tccaaacttt cgagcctcat
    12001 aattacaagt agaaacaacc cctctgccgc cggtttttct gggatcagaa ccaataataa
    12061 caggctttcc tttaagagag ggattgtcac gctcttccac agatgcaaaa aaagcatcca
    12121 tatcaatgtg gataatttta cgcgacgtat catttattaa aggaaaaatt aacattgttc
    12181 tctcccccat tctctctact ccattataac agattacaac ttaatgatac agattaaaaa
    12241 aactgtatta taaaagaatc ataattttgt caagaaatat gcttgcgaaa acgtttactt
    12301 tttcctaaaa tggatatgta aacaaaatta aatctgtcac aatgtttaag gagaaaacta
    12361 tggcaacagt taaaacaaac acagatatct ttgaacaagc ttgggaaggc tttaaaggtg
    12421 ttgattggaa agaaaaagca agtattgctc gcttcgttca agcaaactat gcaccttacg
    12481 atggagatga aagcttctta gctggagcaa ctgaacgttc acttcacatc aaaaaagtga
    12541 ttgaagaaac aaaagctcac tatgaagaaa ctcgcttccc aatggatact cgtgttgctt
    12601 caatttctga attaccagct ggttttatcg acaaagatag cgaactcatc tttggtattc
    12661 aaaacgacga gcttttcaaa ttgaacttca tgcctaaagg tggtatccgt atggcggaaa
    12721 ctacacttaa agaaaatggt tatgaacctg atccagctgt tcacgaaatc ttcactaagt
    12781 acgctacaac agtaaatgat ggtatcttcc gcgcttatac ttctaacatt cgtcgtgctc
    12841 gccatgcaca cactgttact ggtcttccag acgcttattc acgcggacgt atcattggtg
    12901 tatatgctcg tctagctgtt tacggtgctg actacttgat gcaagaaaaa gttaatgact
    12961 ggaatgcatt gaatgatatt gatgaagagt caattcgtct tcgtgaagaa atcaaccttc
    13021 aataccaagc tcttggtgaa gttgtaaaac ttggtgacct ttacggcgta gatgttcgta
    13081 aaccagccat gaacactaaa gaagccattc aatgggtgaa cattgctttc atggctgtat
    13141 gtcgtgttat caatggtgct gcaacatcac ttggacgtgt tcctattgtc cttgatatct
    13201 ttgcagaacg tgacctcgct cgcggtacat tcacagaatc agaaatccaa gaatttgttg
    13261 atgattttgt ccttaaactc cgtactgtaa aatttgctcg tactaaagct tatgatgccc
    13321 tttactcagg tgacccaacc tttatcacaa cttcaatggc aggtatggga gctgatggac
    13381 gtcatcgtgt taccaaaatg gactatcgtt tcttaaatac acttgacaat atcggtaact
    13441 caccagaacc aaacttaact gttctttggt cagaccaatt accatatgct ttccgtcgct
    13501 actgtatgtc aatgagtcat aaacactctt caatccaata tgaaggtgta tcaacaatgg
    13561 ctaaagaagg ctacggtgaa atgtcatgta tttcatgttg tgtatctcca cttgatccag
    13621 aaaacgaaga caaacgtcac aaccttcaat attttggtgc tcgtgttaac gttatgaaag
    13681 cacttcttac tggtcttaac ggtggttatg atgatgttca taaagattac aaagtatttg
    13741 acatcgaccc aattcgtgac gaagtactca actttgacac tgtaaaagct aactttgaaa
    13801 aatctcttga ctggttgact gatacttatg ttgatgcgat gaatatcatc cactacatga
    13861 ctgataaata caactatgaa gctgtacaaa tggcattctt accttcacat gtacgtgcta
    13921 atatgggatt tggtatctgt ggttttgcaa atactgtcga ttcattatct gcgattaaat
    13981 acgctactgt taaaccaatc cgtgacgaag atggttacat ctacgactac gaaacagttg
    14041 gtgacttccc acgttatggt gaagatgatg atcgtgttga ctcaatcgct gaatggttac
    14101 ttgaagcctt ccatggacgc cttgctaaac acaaacttta taaagatgct gaagcaactg
    14161 tttctcttct tacaatcaca tcaaacgttg cttactctaa acaaactggt aactcaccgg
    14221 tacataaagg agtatatctc aatgaagatg gtagtgtaaa cctttctaaa gtagaattct
    14281 tctcaccagg cgctaaccct tctaacaaag ctaaaggtgg ctggttacaa aacttgaatt
    14341 cactttctaa acttgatttc gctcatgcaa atgatggtat ttcattgaca actcaagttt
    14401 ctccacgcgc tcttggtaaa acatttgatg aacaagttga taacttggta acagtccttg
    14461 atggttactt tgaaaacggt ggtcaacacg ttaacttgaa tgtcatggac cttaaagatg
    14521 tctatgacaa gattatgaat ggtgaagatg ttatcgttcg tatctcaggt tactgtgtca
    14581 atactaaata tctaactcct gagcaaaaaa ctgagttaac acaacgtgtc ttccatgaag
    14641 tactttcaat ggacgatgca ttaactaact agtttgtatt cgatgaagta ggtttgatta
    14701 tatcaaacct acttttatct atattatcaa tagtactaat tacttcttta tataacctca
    14761 aggaggggac taatgttaaa tcacaaattt cttcaagttc taaagtatga gggtgtcgtt
    14821 tcgataacaa gctggactga attagctcct cacgttacaa acacttggaa tagttacctg
    14881 actattacag atgaccagcg tatcttagct cctgcagcag gaatgactca tcttgaaaat
    14941 gatctcaata ataattctaa aattattatg actctgggca gccgtgaagt cgagggtcgt
    15001 gatggttatc aaggtacagg ctttagaatt gaaggaactg ctaagctatt agaagcagga
    15061 agcgattttg aaattgttaa aaaaaaatac ccttttctgc gtaaagtatt ggaagtaact
    15121 cccataaatg ttatccaact tttataaaaa acttctccta gaattttcta ggagaagttt
    15181 tttattcaaa gtacgatgtt atcgcctctt gtacatgtcg tcgtttctta atccaaagtg
    15241 gacggtcatc ataggaaaag gttctgtttg tcaaaaaaat agctgctgcc tgcttttgat
    15301 aatttagtgc aataaaagga ccagtataac cagtatggtt tatccaatcc ttatctatat
    15361 cccaagctaa tgatcttgat ttactcaaac tataattatt ccacaaaggt ttcgcaaaat
    15421 catcttttaa ataatgaata gaaaatcttt gtaaatcatc aatagttgaa aataaacctg
    15481 cagaaccagt atgctttcct aataccttag ctttaggatc atgtacaata ccatcgttaa
    15541 taccagctac agttggaaca gcttctactc taggaccaaa agaggtttct ttcatctgaa
    15601 aaggagtaaa aatataacgt ttgaaaagtt tatccaaact atcccctaat acctcttcta
    15661 acataaaacc aagtaaaaga aaattaatat cagtgtattt aaacgattta tcttccaaaa
    15721 ccttgatatg attaatggca tctttcagct gagtagcatt caactgatca cgatttggaa
    15781 taaaaggatc aatcccactg ctatgtgtag ctagttgcct tacagttaac gtctgatgat
    15841 gaaacgtagg gtaataatac cttaatggct tatcaatatc aatagtccct tgataaacta
    15901 actttgctag taaagttccc acgccgacaa cttttgatac actggctaga tcatagacca
    15961 atcccgcttt aactttctca ttgccatcaa ttgtccccaa gtaatgttcg tgccactctc
    16021 cattttcata aatagctaag cttgcaccat gataaagatt atctttgatt tgctctttaa
    16081 tgagttctaa aacggtatta gttttaatca ttcttttgaa atccaaatct caatattact
    16141 agtgtcagag atgactaaaa tcttttcttt agaatcaagg taaacatcaa gtcccaatga
    16201 ttcaaaagtt gatttcaagt cgtttaaatt agtatcttca ttaacacagc attctaatat
    16261 ctcaatatcc caagtttcat ttggagcaat ttgcaaatct tgtccttgtg cttctttgaa
    16321 agataaatta attgggaact tcccagcaaa tactttatcg taaaaagctt cagctttcac
    16381 ggcatcaggt acattcaaac taatagattg gaaaacaaag tttgatagac ctgtagtgtc
    16441 atcatttttt tctaactctg gacgctcaat tgctactaat tgtgacaaat catcttcagc
    16501 atgaagtaag aaaaggtctc cctcgggcga aactgtttca taggcataac cattttgacc
    16561 ttgatatact tgagtggttt gcgcaccatt tgccaaaagc ttttcaatat cttttgcatc
    16621 ttgagactta acaataatct ttgctaactt cttagtacca ttcaccgcac gagtacgata
    16681 agttggggat tcctcaataa taaagctagc ctctttgttc tgccatgctg agaaaactgc
    16741 gattgcattt tcttcactta tcaatttaaa tccaagagac tcctgataaa atgcaatatt
    16801 caagtctcta ttattaacac gtaatactgg gtgtttaaat gtcaatgaat gaaataacgt
    16861 cataaattcc tccttgttca gtctagtcta ttttagataa ttttagataa tttagcaaga
    16921 atctttgtgc tctcaatacc ataaagtcaa attgacagtt tcactatttt tttgtagact
    16981 ggatattatg aatgaaacac tactattaca tgggattcag cttattctga ttatcgccat
    17041 gattatcact ttttatcaga ttgttagaca tattaggtct caaaaaataa atcctttcaa
    17101 acgatttttc actggtttgt ggatcggttt tgtaactgat gctctcgata ctcttggtat
    17161 cggctctttt gcaacaacga ccacattttt taaactaact aagcttgttg aagacgatcg
    17221 taagataccc gcaacaatga cagctgcaca cgtacttcca gttttgttgc agtcactctg
    17281 ttttattttt gttgtaaaag tagaggcttt aacactaata acaatggctg gtgcagcttt
    17341 tattggggct tttgttggag ctaaaatgac taaaaattgg cacgctccta ctgttcaacg
    17401 tattctagga acactgctca tcacagctgc tattattatg ctctatcgta tgatcactaa
    17461 ccctggcgct ggtatttcag attcagtgca tggtctacac ggcatttggt tatttgttgg
    17521 tatcgtcttc aattttatta ttggtgtatt aatgactatg ggactaggta attatgctcc
    17581 tgaacttatc ttcttctccc taatgggact gagtcctgca gttgctatgc ctgtcatgat
    17641 gctagatgca gctatgataa tgacagcttc tagtacacaa tttatcaaga gtggtcgtgt
    17701 taattggaac ggctttgccg gtctggttac tgggggaata ttaggcgtta ttgtcgctgt
    17761 tctcttctta actaaccttg accttaacag cttgaaaaca ctggttgtag gtattgtttt
    17821 atttactgga gcaatgttaa tacgttcttc ctttaaaaaa actatgccca aaagtcatca
    17881 ttaaacaaaa tcagctagca agactggctg atttttatat ttaaaacaaa aaaagaccgg
    17941 taaaccgatc tttccttaac tattaaagat ataatagttt gaacaaggct acagcgagta
    18001 tacttgctac gattggtgct aaaactggca cccatgcata ccaccatttt gaatcttctt
    18061 tagcttgtcc aagaatttgt tttggtaata agcggtgaac aatacgtgga ccaaggtcac
    18121 gcgcagggtt caatgcaggt ccagtcggtc cacctaaaga agcaactaga gtcattacca
    18181 agaaaccaat acctaagtga gctacagcaa gtgaaccagt tacgtatggt gaaatccttg
    18241 tcgctgctgt cgtttggtca taaccagctt gaacaagttt accaacaagt tctacaccga
    18301 aatagttctt cgttaaagcc aaagctccaa agaaaagaac aaaagaacca acgaattcat
    18361 ttaagaaacc attaatgtaa gccgctttac gacttgattt ctgtccatca tctaatgcag
    18421 aaatagttga aaatgatcca agaacatgat ttggattttc tgtcttaaca aagtaaggtt
    18481 gataaaccat aacgacaact aactgtccaa acattgctcc caaaatttgt gcaagaatgt
    18541 attgtccaac gtgtgcccaa gggaaaagac ctgaaaatgc caatccaagt gtgaaagctg
    18601 ggttaatatg gtttccagag acattaccaa acataagcgc tggcatcata acacctaaac
    18661 cataaccaat tgcaataata atccaacctg aattatttcc ttttgtgcct tttaagtcaa
    18721 cgttagctac agccccattt cctaagatga taagaaaagc ggttgcgata aattctgtga
    18781 tatacttcac agtccaagtg atttcgatca atccttgttt cctcccaaaa aaatatttac
    18841 aacattaacc attctaccaa gtaagcttaa tattgtaaag cttatcacaa aataaaatta
    18901 ataa
//
LOCUS       DBJYSH010000033        18837 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.033, whole genome shotgun sequence.
ACCESSION   DBJYSH010000033 DBJYSH010000000
VERSION     DBJYSH010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 18837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18837
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.033"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            37..762
                     /locus_tag="ACUBV3_000879"
     CDS             37..762
                     /locus_tag="ACUBV3_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HHU3847395.1"
                     /translation="MLKIENVTGGYVNIPVLKNISFEVNDGELVGLIGLNGAGKSTTI
                     NEIIGILRPYQGDITIDGISLEANQELYRKKIGFIPETPSLYEELTLREHLETVAMAY
                     DIATDEVMARAQKLLEMFRLTDKLDWFPMHFSKGMKQKVMIICAFVVSPSLFIVDEPF
                     LGLDPLAISDLINLLAEEKAKGKSILMSTHVLDSAEKMCDRFVILHKGEIRAVGTLEE
                     LRAIFGDSNANLNDIYIALTKEV"
     gene            764..1798
                     /locus_tag="ACUBV3_000880"
     CDS             764..1798
                     /locus_tag="ACUBV3_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HHU3847396.1"
                     /translation="MKKLFNKRRSLFLTQNSKYLRYVFNDHFVLVLMFLSGFLLYQYS
                     QLLKDFPKTHWPIIVIVSIIILMLLAMGGIASYLEPADKQFLLIKEEAIKEIINSAKK
                     RTYIFWLVIQTLFLVLISPILIKLGLSVFMITLLIFGLGIIKWLVITYKVKVFYNNQN
                     LNWDAAINHEQERKQSILKFFSLFTNVKGISTSVKRRSFLDVILKLISKTPSRLWTNL
                     FVRAFLRSSDYLGLTIRLVTLNILSVIFVNETYLALALAFVFNYLLLFQLLALGHHFD
                     YQYMNQLYPVRLNAKASQLKGFLRVLSYAVTVIDIILIRELEPVILLIVLMLIVTEYY
                     IPYKIKKMID"
     gene            1856..2656
                     /gene="ccrZ"
                     /locus_tag="ACUBV3_000881"
     CDS             1856..2656
                     /gene="ccrZ"
                     /locus_tag="ACUBV3_000881"
                     /EC_number="2.7.1.15"
                     /EC_number="2.7.1.229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000155919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0051726 - regulation of cell cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell cycle regulator CcrZ"
                     /protein_id="HHU3847397.1"
                     /translation="MTISNQELTLTPLRGKSGKAYIGTYPNGERVFVKYNTTPILPAL
                     AKEQIAPQLLWARRTSNGDMMSAQEWLDGRTLTKEDMGSKQIIHILLRLHKSRPLVNQ
                     LLQLGYKIENPYDLLMDWEKQTPIQIRENTYLQSIVTELKRSLPEFRTEVATIVHGDI
                     KHSNWVITTSGLIYLVDWDSVRLTDRMYDVAYILSHYIPQKHWKDWLSYYGYKDNEKV
                     WSKIIWYGQFSYLSQIIKCFDKRDMEHVNQEIYELRKFRELIKKHNAS"
     gene            2646..3281
                     /gene="trmB"
                     /locus_tag="ACUBV3_000882"
     CDS             2646..3281
                     /gene="trmB"
                     /locus_tag="ACUBV3_000882"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HHU3847398.1"
                     /translation="MRVRKRKGAEEHLENNPHYVISNPEEAKGRWHEIFGNNNPIHIE
                     VGSGKGAFITGMAEQNPDINYIGIDIQLSVLSYALDKVLDSGAKNIKLLLVDGSSLSN
                     YFDTGEVDLMYLNFSDPWPKKKHEKRRLTYKTFLDTYKDILPEQGEIHFKTDNRGLFE
                     YSLASFSQYGMTLKQVWLDLHASDYQQNIMTEYERKFSNKGQVIYRVEARF"
     gene            3325..3411
                     /locus_tag="ACUBV3_000883"
     tRNA            3325..3411
                     /locus_tag="ACUBV3_000883"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3359..3361,aa:Ser,seq:gga)
     gene            3770..4246
                     /gene="rimP"
                     /locus_tag="ACUBV3_000884"
     CDS             3770..4246
                     /gene="rimP"
                     /locus_tag="ACUBV3_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018366632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HHU3847399.1"
                     /translation="MANQTIVDIVTQVVTPAIQAPFELVDVEYEKMGGDYVLSILIDK
                     PGGITVEDTAQLTDVVSPLLDTIQPDPFPEQYMLEVSSPGLERPLKTAEALSNAVGSY
                     INVSLYKSIDKVKIFEGDLLSFDGETLTIDYMDKTRHKTVDIPYQTVAKARLAVKL"
     gene            4282..5433
                     /gene="nusA"
                     /locus_tag="ACUBV3_000885"
     CDS             4282..5433
                     /gene="nusA"
                     /locus_tag="ACUBV3_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HHU3847400.1"
                     /translation="MSKEMLEAFRILEEEKHINKEDIIDAVTESLKSAYKRRYGQSES
                     CVIEFNEKKADFTVYTVREVVDEVFDSRLEISLKDALAISSAYELGDKIRFEESVTEF
                     GRVAAQSAKQTIMEKMRRQMREVTFNEYKQHEGEIMTGTVERFDQRFIYVNLGSLEAQ
                     LSHQDQIPGESFKSHDMIDVYVYKVENNPKGVNVFVSRSHPEFIKRIMEREIPEVFDG
                     TVEIMSVSREAGDRTKVAVRSHNSNVDAIGTIVGRGGSNIKKVISNFHPKRVDAKTGL
                     EIPVEENIDVIQWVEDPAEFIYNAIAPAEVDMVLFDDEDTKRATVVVPDSKLSLAIGR
                     RGQNVRLAAHLTGYRIDIKSASEYEKMEAQELQTEEVAQESEVISDKAE"
     gene            5455..5751
                     /gene="rnpM"
                     /locus_tag="ACUBV3_000886"
                     /gene_synonym="ylxR"
     CDS             5455..5751
                     /gene="rnpM"
                     /locus_tag="ACUBV3_000886"
                     /gene_synonym="ylxR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006531117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase P modulator RnpM"
                     /protein_id="HHU3847401.1"
                     /translation="MAKTKKIPLRKSVVSGEVIDKRDLLRIVKNKEGQVFIDPTGKQN
                     GRGAYIKLDNDEAILAKKKRVFDRSFSMEVSDEFYDELLAYVDHKVKRRELGLE"
     gene            5744..6046
                     /locus_tag="ACUBV3_000887"
     CDS             5744..6046
                     /locus_tag="ACUBV3_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HHU3847402.1"
                     /translation="MNNSEKVLNLIGLAQRAGRLITGEELVIKAIQNQQVSLIFLAND
                     AGPNLTKKVTDKSNYYKTEVSTVFSTLELSDALGKPRKVVAVADAGFSKKMRTLMA"
     gene            6066..6222
                     /locus_tag="ACUBV3_000888"
                     /pseudo
     CDS             6066..6222
                     /locus_tag="ACUBV3_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376979.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 N-terminal
                     domain-containing protein"
     gene            6348..8849
                     /gene="infB"
                     /locus_tag="ACUBV3_000889"
                     /pseudo
     CDS             6348..8849
                     /gene="infB"
                     /locus_tag="ACUBV3_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018381344.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
     gene            8958..9308
                     /gene="rbfA"
                     /locus_tag="ACUBV3_000890"
     CDS             8958..9308
                     /gene="rbfA"
                     /locus_tag="ACUBV3_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HHU3847403.1"
                     /translation="MANHRIDRVGMEIKREVNEILRFRVNDPRVQDVTITDVQMLGDL
                     SMAKVFYTIHSTLASDNQKAQIGLEKATGTIKRELGKNLTMYKIPDLQFVKDESIEYG
                     NKIDEMLRNLDKKD"
     gene            complement(9392..10396)
                     /locus_tag="ACUBV3_000891"
     CDS             complement(9392..10396)
                     /locus_tag="ACUBV3_000891"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HHU3847404.1"
                     /translation="MKPSFKKLLLLFSIITILSIACTPHAKASGRSWKSWFIEQYFWL
                     KRDKSYYKVQDESSFQKYLNASREQSDKGYYLDPNSVNGGLVQERLFDMQVYSWNDNG
                     KANQKTIFYLAGGSYLNNPTPYHISMLKTLSTSLDAKIILPIYPKTPRYTYDYAIPRL
                     VNPYRHFHEKNANLTLMGDSAGGGLALGLAHALSHQSGQEAIPQPKNIILLSPWLDVT
                     MKHPEIPKYEDTDPILSAWGLARVGEIWANGSNNTNHTYVSPKNAPATKLAPITLFTG
                     TREIFFPDIRDYAAQLQAANHPVNYIAQEGMNHVYPIYPIEEAKTAQYQMIDIINKTP
                     "
     gene            10559..10975
                     /locus_tag="ACUBV3_000892"
     CDS             10559..10975
                     /locus_tag="ACUBV3_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HHU3847405.1"
                     /translation="MTISNAEWEIMRVVWAQQNTTSNEILAVLLEKYDWTPSTVKTLL
                     RRLLDKGYVSREKMGKGFSYSPLIDEDLAMMSEVDSVLQKVCQTKHVAIVRHLLESIP
                     MTEKDRLNLQASLEAKKGKTLERVACNCIPGQCQCH"
     gene            10988..13222
                     /locus_tag="ACUBV3_000893"
     CDS             10988..13222
                     /locus_tag="ACUBV3_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HHU3847406.1"
                     /translation="MANRKETFLIDGMTCASCALTIEKAVNKLDHVDSAVVNLATEKM
                     TVTFDDTTLSPNVIEECVSETGYEASLFKEETSKSQSERHQLAIEKMWHRFWMSAVAT
                     IPLLYISMGPMINLWLPSFLMPDKGPLNYGVIQLLLTLPVMYFGRIFYQNGFKALFKR
                     HPNMDSLVAIATTAAFIYSLYGLYEILQGDIHYAHQLYFESVAVILTLITLGKYFEIL
                     SKGRTSASIEKLLTLSAKEARVIKDGEDYMVPLDKVKIGETILVKPGEKIPLDGHVVA
                     GESSIDESMLTGESIPVEKKVGSKVYGASINGQGSLTIFVEKEAGGSLLSQIINLVEV
                     AQASKAPIANLADKVSGVFVPFVIVIAILSGLSWYLILGQSFAFSLKIMIAVLVIACP
                     CALGLATPTAIMVASGKAAENGILFKGGEVLEKAHHIDTIVFDKTGTLTKGKPEVVAI
                     KTYGVDKEEFLGQVASVEKLSNHPLSQTIVNKAKEKELPLREVMAFKNILGYGLSAII
                     NGKTMLVGNANLMTKNDVNLDLAKADIEIAQEEAQTVVYVSENGVLSGLITLTDQLKT
                     DSQETVKQLQRLGFNLVLLTGDNKASADAIAQKLGITTVVSEVLPDQKANVILELKEK
                     GGQIAMVGDGINDAPALASSDVGISMSSGTDIAIESADIVLMKPELTDLLKAMTISKQ
                     TIQIIKENLFWAFFYNVLAIPVAMGVLHLFGGPLLNPMLAGLAMAFSSVSVVLNALRL
                     KVLK"
     gene            13263..13469
                     /locus_tag="ACUBV3_000894"
     CDS             13263..13469
                     /locus_tag="ACUBV3_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HHU3847407.1"
                     /translation="MKHTYRVSGMKCDGCAKTVSDKLSSVMGVDEVNVDLTKNQVVVS
                     GKTFKCLLKRSLKDTKYSLEEDIN"
     gene            13579..14193
                     /locus_tag="ACUBV3_000895"
     CDS             13579..14193
                     /locus_tag="ACUBV3_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000020717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HHU3847408.1"
                     /translation="MSIDIWNILSLVGTVAFASSGAIVAIEEEFDVLGLFILGFVTAF
                     GGGAIRNVLIGLPIETLWSQGIAFYAAAAAILFIMIFPNLLSGKGRDAEVVSDAIGLA
                     AFSVQGALYATQSHQPLSAVIVAAVLTGAGGGIVRDVLAGRKPGVLRSEIYAGWSILV
                     GIILYFKIAKTTTDYYLLVLVVTSLRMLGYKKQWHLPVVRPRKK"
     gene            14208..15020
                     /locus_tag="ACUBV3_000896"
     CDS             14208..15020
                     /locus_tag="ACUBV3_000896"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HHU3847409.1"
                     /translation="MIKLIAIDMDGTLLNDEKKIPKENIQAIKEATQAGIKIVLCTGR
                     PMSGILPYFNELGLTKEEYIIMNNGCSTYSTKDWQLIDSATLTHDELIFLEEVVKEFP
                     NVCLTLTAENTFYAVGEEVPEIVAYDADLVFTKAKSISLDALRNQEEIVFQAMYMGLD
                     ADVTAFQEAVEEALISKFSGVRSQDYIYEIMPQGVTKARGLKSLIAKLGLDINQVMAI
                     GDAPNDIELLDLVPNSVAMGNASDEIKSRCKYITVDNNQAGVAKAIYDYALR"
     gene            15133..17775
                     /gene="polA"
                     /locus_tag="ACUBV3_000897"
     CDS             15133..17775
                     /gene="polA"
                     /locus_tag="ACUBV3_000897"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019314525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HHU3847410.1"
                     /translation="MTNKNKLLLIDGSSVAFRAFFALYNQIDRFKNNSGLHTNAIYGF
                     HLMLNHILGRVQPSHILVAFDAGKTTFRTEMYADYKGGRAKTPDEFREQFPYIRQQLD
                     VLGIKHYELEHYEADDIIGTLAKQAEASKEHFDITVVSGDKDLIQLTDTNTVVEISKK
                     GVAEFEEFTPAYLMEKMGITPSQFIDLKALMGDKSDNIPGVTKIGEKTGLKLLSEYGS
                     LEGIYENIEAMKQSKMKENLINDKEQAFLSKTLATINIASPITIGLEDILYSGPQDIK
                     ALSQFYDEMDFKQFKAALGEETSQEDFEVDFTEVEQLKPEMFSDNDFYYFEMLGDNYH
                     VEDLIGIAWGNSDTIYATSDVSLLQEALFKKALSKPIKTYDFKRSKVLLNRFNIDLPE
                     PAFDTRLAKYLLSTTEDNLVSTIARLYTNLPLDTDDAVYGKGAKRAIPEKTRFLEHLA
                     KKVKVLVDSEANIMQQLKANEQEELLFEMEQPLANVLAKMEIRGIKVKKNTLNEMAIE
                     NQKVIETLTQEIYELAGQEFNINSPKQLGKLLFETLGLPVEMTKKTKTGYSTAVDVLE
                     RLAPISPLVTKILEYRQITKLQSTYIIGLQDYILEDGKIHTRYVQDLTQTGRLSSSDP
                     NLQNIPVRLEQGRLIRKAFVPSEDNAVLLSSDYSQIELRVLAHISKDEHLIAAFKEGA
                     DIHTSTAMRVFGIEKPENVTPNDRRNAKAVNFGIVYGISDFGLSHNLGIPRKLAKQYI
                     DTYFERYPGIKNYMETVVREAKDKGYVETLFHRRRSLPDINSRNFNIRQFAERTAINS
                     PIQGSAADILKIAMINLDRVLYKGGYKSKMLLQVHDEIVLEVPNEEIGAIKELVTKTM
                     ESAISLSVPLIADENAGETWYEAK"
     gene            17805..18245
                     /locus_tag="ACUBV3_000898"
     CDS             17805..18245
                     /locus_tag="ACUBV3_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HHU3847411.1"
                     /translation="MVYHFQNPSDFMLKNYLTKAKTIAVVGLSDRQETAAYQVSKIMQ
                     EAGYQIIPVNPKNAGQKILGQMTYASLKDVTEHIDIVNIFRRSEYLPDIAREFLEVDA
                     DIFWAQLGLESQEAETILKQAGHKQIVMNKCLKVEYQKLHSETN"
     gene            18327..18806
                     /gene="perR"
                     /locus_tag="ACUBV3_000899"
     CDS             18327..18806
                     /gene="perR"
                     /locus_tag="ACUBV3_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide-responsive transcriptional repressor
                     PerR"
                     /protein_id="HHU3847412.1"
                     /translation="MDSHSHGHRPLDAYENVLEHLREKRIRITETRKAIISYMVNSRE
                     HPSAEKIYNDLLPEYPNMSLATVYNNLKVLVDEGFVTELKLCNYSTTYYDFMGHQHLN
                     IACEDCGKIVDFVDVDLLDISREAHQQTGFEVTRVQLVAYGICPECQRKRKDKYKKD"
BASE COUNT         6328 a         2872 c         3889 g         5748 t
ORIGIN      
        1 agttaataaa gtatatcaaa gatagagaga atgacaatgt taaaaattga aaatgttaca
       61 ggggggtatg ttaatatccc agttttaaaa aatatttctt ttgaagtaaa tgatggagag
      121 ttagttggat taattgggct taatggtgca ggaaaatcaa ccactattaa cgagattatt
      181 ggtattttga gaccctatca aggagatatt accattgatg gcattagtct tgaagccaac
      241 caagaacttt atagaaagaa aataggcttt attcctgaga caccaagtct ctatgaggaa
      301 ctaactctca gggaacattt agaaacggta gctatggcgt atgatattgc caccgatgaa
      361 gttatggcac gtgcgcaaaa actattagag atgtttcgtc taacagataa attagactgg
      421 tttccaatgc atttttcaaa gggaatgaaa caaaaggtta tgattatttg tgcttttgtt
      481 gtatctccaa gtttatttat tgttgatgaa ccatttttag gtttggatcc actagctatt
      541 tcagatttga ttaatcttct agcagaagaa aaagctaaag ggaaatctat tttgatgtca
      601 acacacgtat tagattctgc cgaaaaaatg tgtgatcgtt ttgttatttt acataaagga
      661 gaaatacggg cagttggaac cctagaggaa ttacgggcta tttttggaga cagtaatgct
      721 aatttaaatg acatctacat agctctgaca aaagaggtgt gatatgaaaa agttatttaa
      781 taaacgtcga agtctctttt taactcaaaa tagtaaatat ttgagatacg tctttaatga
      841 tcattttgta ttagtattaa tgtttttgtc aggttttttg ttatatcagt atagccaatt
      901 actaaaagac tttccaaaaa cacattggcc aattatcgtg attgtgtcaa tcattatctt
      961 aatgttacta gcgatggggg gaatagccag ctatttagaa ccggctgata aacaattttt
     1021 acttataaaa gaagaagcaa taaaggaaat tataaattct gctaaaaaac ggacttatat
     1081 cttttggttg gttattcaaa ccttattttt agtattaata tcaccaattt tgataaagtt
     1141 aggcttgtca gtttttatga ttacattgtt aatttttggt ctaggaataa tcaaatggtt
     1201 ggtgataact tacaaggtaa aggtctttta taacaatcaa aatttgaact gggatgcagc
     1261 tataaatcat gagcaggaaa gaaaacagag tatattaaaa tttttctctt tgtttacaaa
     1321 tgttaaggga atttcgacaa gtgtaaaacg tcgttcattt ttagatgtta ttttaaaact
     1381 tatttctaaa acgccatcgc gtttatggac taacttattt gtgagggcat ttctaagaag
     1441 ctcagattat cttggtttaa caataaggtt ggtaacttta aatatcttat cagtcatctt
     1501 tgttaacgaa acctacctcg cccttgctct ggcatttgta tttaactact tattattgtt
     1561 ccaattatta gctttaggtc accattttga ttatcaatac atgaatcagc tctatccagt
     1621 tagattaaat gctaaagcaa gtcagctaaa aggttttctc agagtattat catatgctgt
     1681 gacagtaatt gatatcatac ttataagaga attggaacct gttatattat tgatagtttt
     1741 gatgttaatt gtgacggaat attacatccc ttacaaaata aagaagatga ttgactaaag
     1801 cctataaaac aagtaaaata agaggaaacg actaaatgaa aaggagggac tgctggtgac
     1861 aatatcaaat caagaattga cgcttactcc cttaagagga aaaagtggca aagcctatat
     1921 tggtacttat ccaaatggtg aacgtgtctt tgtcaaatat aatacgacac ctattttgcc
     1981 agccttagct aaggaacaga ttgcgccaca actactgtgg gcacgccgta caagtaatgg
     2041 cgatatgatg agtgctcaag aatggttaga tggtcgaaca ttgactaaag aagatatggg
     2101 tagtaaacag attattcata ttttattgcg cctgcacaag tctcgtccct tagtaaatca
     2161 actgttacag ttagggtata aaattgaaaa cccttatgat ttgttgatgg attgggaaaa
     2221 acaaactcct attcaaatcc gagaaaacac ttatttgcaa tctattgtga ctgagttaaa
     2281 gcgtagttta cctgagtttc gaacagaggt tgcaacaatc gttcacggag atatcaaaca
     2341 tagcaactgg gttattacaa caagtggttt aatttattta gttgattggg attctgttcg
     2401 tttaacagat cgcatgtatg acgttgcata tattttaagt cattatattc cacaaaaaca
     2461 ttggaaagat tggctgtctt actacggtta taaagataat gagaaagtct ggagcaaaat
     2521 tatttggtat gggcaatttt catatttatc acagataatc aaatgttttg ataaacgaga
     2581 tatggagcat gtcaatcaag aaatttatga attacgaaag tttagagagt taattaaaaa
     2641 gcataatgcg agttagaaaa cgtaagggtg cagaggaaca cttagaaaat aatcctcact
     2701 atgttatttc aaatccagaa gaagccaaag ggcgttggca tgagattttt ggaaataata
     2761 accctattca catagaagtt ggttccggaa aaggggcttt tatcactggt atggcagagc
     2821 aaaatccaga tatcaattat attggaattg atattcaatt gtcagtgctt tcctatgctc
     2881 tggataaagt tctagatagc ggtgctaaga atatcaaatt actattagtt gatggttcta
     2941 gtctatcaaa ctactttgat actggcgaag ttgatttgat gtatcttaac tttagtgatc
     3001 catggcctaa gaaaaagcat gagaaacgtc gattgaccta taagactttc ttggatacct
     3061 ataaagacat acttcctgag cagggggaaa ttcattttaa aacagataat agaggtttat
     3121 ttgaatatag tcttgctagc ttttctcaat acggtatgac tttaaaacaa gtttggttag
     3181 atttacatgc aagtgattac cagcaaaata tcatgactga gtacgaacgc aaattttcaa
     3241 ataaaggaca ggttatctat cgtgtagaag cacgatttta gaataagtaa atagtaagtg
     3301 ggtagttatt tgctactcct ttatggagag gtcgcatagt ggctgagtgc gcacgcttgg
     3361 aaagcgtgta gtccttaatg gggctcgggg gttcgaatcc cctcctctcc tttaatcttg
     3421 ttaatgtatc agtgtttatt aagtgttcac ctttttcact cgtcaaaagt ccaagtttcg
     3481 aaaaatttct tttattttat catcgttttt ctttgagctt gaagtattag cataaagttt
     3541 tctaagctct tacaaagaac tgaggacaaa attttaattt tttgatataa gattagtgta
     3601 cggtgatgct aatttaaagt aaatgattgc aataatcgta ttttgtgcta taatagtatt
     3661 aaataataca gagaggggcg gagttttact tcgcctctta tgtgtgtgat tcatagaatt
     3721 tatagtcatt ataagttcta aagcaagtta gaaagaagag gtagctgtta tcgcaaatca
     3781 aaccattgtt gatattgtta ctcaagtcgt aactcctgct attcaagcac cgtttgaatt
     3841 agtagatgta gaatacgaaa aaatgggtgg cgactatgtg ctcagtattt taattgataa
     3901 acccggaggc attacggttg aagatacagc acaattgaca gacgttgtaa gtcccttact
     3961 tgacacaatt caaccagatc catttccaga acagtatatg cttgaagtct caagtcctgg
     4021 gttggaaagg ccacttaaaa cagcagaagc attatcaaat gctgttgggt cttatatcaa
     4081 tgtaagtctg tataaatcca ttgataaagt taaaatattt gaaggtgact tattatcatt
     4141 tgatggtgaa accttgacaa ttgattatat ggacaaaaca cgtcataaaa ctgtagatat
     4201 tccctatcag acggttgcaa aagcccgtct tgcggttaaa ctttaagaaa ggatgattct
     4261 tgcgaagcaa gaatggacac tatgagcaaa gaaatgctag aagccttccg tattttggaa
     4321 gaagaaaaac acattaataa agaagatatt attgatgcag ttactgagtc actaaaatct
     4381 gcctataagc gtcgttatgg tcaatcagaa tcatgcgtta ttgaatttaa cgagaagaaa
     4441 gcagacttta ccgtctatac tgttcgtgaa gttgttgatg aagtttttga tagccgtttg
     4501 gaaattagcc taaaagacgc acttgctatt agctcagcgt atgaacttgg tgataagatt
     4561 cgctttgagg aatctgttac agaatttgga cgcgtggcag ctcagtcagc aaaacaaact
     4621 attatggaaa aaatgcgtcg tcaaatgcgt gaagtaacct tcaatgaata caaacaacac
     4681 gaaggtgaaa ttatgactgg tactgttgaa cgatttgacc aacgttttat ctatgtcaat
     4741 ctcggttcac ttgaggcgca attatcgcat caagatcaga ttcctggaga gagctttaaa
     4801 tctcatgata tgattgacgt ttatgtctat aaagtggaga ataatcctaa aggagtcaac
     4861 gtttttgtta gccgtagtca tccagagttt atcaaacgca ttatggagcg agaaattcca
     4921 gaagtatttg atggaactgt tgagattatg agcgtctctc gtgaggctgg agatcgtact
     4981 aaagttgctg tacgcagtca taattcaaat gttgatgcga ttggaacaat tgttggacgc
     5041 ggtggaagta acattaaaaa agttatcagt aatttccatc ctaaacgcgt tgatgctaaa
     5101 acaggattgg agatcccggt tgaagaaaac attgatgtta ttcaatgggt tgaagatcca
     5161 gcggaattca tttacaatgc tattgcacca gccgaagttg atatggtact ttttgatgat
     5221 gaagatacaa aacgtgctac tgtagttgtt ccagatagca agttatcgct agctattggt
     5281 cgccgaggac aaaatgtgcg tttagcagct catttgactg gctatcgtat tgatattaaa
     5341 tctgcaagtg aatacgaaaa aatggaagcg caagaacttc aaactgagga agtagctcaa
     5401 gaatctgaag ttatttcaga taaggcagaa taatataaat gagagaggtg ttttatggct
     5461 aaaacaaaaa aaataccttt aagaaaatca gttgtttctg gtgaagtgat cgataagcga
     5521 gatcttttac gaattgttaa aaataaagaa ggacaggttt ttatagatcc taccggaaag
     5581 caaaatggtc gaggtgccta cataaaattg gacaatgatg aagctatcct agcgaaaaag
     5641 aaacgagtgt ttgatcgcag tttttcaatg gaagtttctg atgaattcta tgatgaacta
     5701 ttggcatatg tcgaccataa ggtcaaaaga agagagttag gccttgaata attcagaaaa
     5761 agtattaaat ctcattgggt tagcacagcg tgcggggcgc cttattacag gtgaagaatt
     5821 ggttattaaa gcaattcaaa atcaacaagt atcgctaatc tttctagcaa atgatgctgg
     5881 cccaaatttg acgaaaaaag taactgataa aagtaattac tacaaaacag aagtctccac
     5941 agtgttttca acactggaat taagtgatgc gttaggcaaa ccaagaaaag tggttgctgt
     6001 cgcagacgct ggattttcaa agaaaatgag gactcttatg gcataacaga ataggaggac
     6061 atcaattgtc aaagaaaaga ttacacgaaa tcgctaagga aattggaaaa acgagtaagg
     6121 aagtggttga gcaagctcaa tcgcttggct tacctgtaaa aagtcatgct tctagcgttg
     6181 aagaaaatga tgctacacgt attgttgaga gttttagctc ttctaaaact aaagctccaa
     6241 caaattctgt tcaaactaat caaggtgtga aaacagaatc taaaactgtt gaaaccaaac
     6301 aaggcttgtc cgatgataaa ccttcaactc aacctgtagc taaaccgaaa ccacaaagtc
     6361 gtaactttaa ggcggagcgc gaggctcgtg ctaaagctga agcggagaag cgtcaacata
     6421 atggggatca ccgtaagaat aatcgccata atgatacacg ctctgacgac agacgccatc
     6481 aaggtcaaaa gcgttcaaat ggtaatcgta atgataaccg acaaggtcag caaaataacc
     6541 gtaataaaaa tgatgggcgt tatgctgatc ataaacagaa accccaaacg cgtcctcagc
     6601 aacctgcagg caatcgcatt gactttaaag ctcgagcagc agctttgaaa gctgagcaaa
     6661 atgctgaata ctcacgtcat agtgagcagc gtttccgtga agagcaagaa gccaaacgtc
     6721 aagcagctaa ggagcaagaa ctagctaaag cagcagcgtt aaaagcgcaa gaagaagctc
     6781 aaaaagctaa ggaaaaactt gcttctaaac ctgttgctaa ggtaaaagaa attgtcaata
     6841 aagtagccgc tacgccatca cagacagcag atagtcgtcg taaaaaacaa actcgttctg
     6901 ataagtctcg tcaattttca aatgaaaatg aagatggtca aaagcaaaca aaaaataaga
     6961 aaaactggaa taatcaaaac caagtgagaa atcaaagaaa tagtaactgg aatcataata
     7021 agaaaaataa aaaaggtaag actaatggag ctccaaaacc agtaacagag cgtaaattcc
     7081 atgaattacc aaaagaattt gaatatactg agggcatgac cgttgcggaa attgcaaaac
     7141 gtattaaacg cgaaccagca gaaattgtta aaaaactctt tatgatggga gttatggcaa
     7201 cgcagaatca atcattagat ggagacacta ttgagctatt gatggttgac tacgggattg
     7261 aagcacatgc taaggttgaa gttgatgaag ctgatattga acgcttcttt gcagatgagg
     7321 attaccttaa tcctgataac ttaacggaac gtccgcctgt tgttactatc atgggacacg
     7381 ttgatcatgg taaaacaact cttcttgata ctctacgtaa ttcacgtgtc gctactggcg
     7441 aagctggtgg aattactcaa catattggtg cttatcagat tgaagaagct ggcaagaaaa
     7501 ttactttctt ggatacacca gggcatgcgg cctttacttc tatgcgtgcg cgtggtgcat
     7561 cagtaactga tattactatc cttatcgttg ccgctgatga tggtgttatg ccgcagacag
     7621 ttgaggcaat taatcactct aaagctgcgg gtgtcccaat tattgttgct atcaacaaaa
     7681 ttgataaacc tggtgcaaat ccagaacgcg ttatcagtga attagctgag catggtgtca
     7741 tctctactgc atggggtggt gagtctgaat ttgttgaaat ttcagctaag tttggtaaaa
     7801 atattcaaga attacttgag acagttcttt tagttgctga aatggaagaa ttaaaagcag
     7861 atgcagatgt tcgtgctatt ggtactgtta ttgaagcccg tcttgataaa ggtaagggag
     7921 ccgttgcaac gcttcttgtc caacaaggta ctttaaatgt acaagatcca atcgttgttg
     7981 gtaatacatt tggacgtgtt cgtgcgatga ctaatgatct tgggcgtcgt gtcaaagttg
     8041 cgggtccatc tacaccagtt tctattactg gtttgaacga agctccaatg gctggtgatc
     8101 attttgctgt ttatgcagat gagaaagctg cgcgtgctgc aggtgaagaa cgtgcaaaac
     8161 gtgctctctt aaaacaacgc caaaacacgc aacgtgttag cctcgagaac ttatttgata
     8221 cccttaaagc tggtgaagtt aagtcagtta atgttattat caaagctgat gtgcaaggtt
     8281 ctgtagaagc acttgtagct tctctcctta agattgatgt tgaaggagta aaagttaatg
     8341 ttgttcactc agcagttggt gctattaatg agtctgatgt aactcttgct gaagcatcaa
     8401 atgctgttat tataggattt aacgtacgcc ctactccaca agctcgtcaa caagccgatg
     8461 ctgatgatgt cgaaattcgt caacatagca ttatctataa agttatcgaa gaagttgaag
     8521 aagcgatgaa aggtaaactt gatcctgaat accaagaaaa aatccttggt gaagccatta
     8581 ttcgtgagac ctttaaagtt tcaaaagtgg gaactattgg tggatttatg gttatcaatg
     8641 gtaaagttac tcgtgattca agtgttcgtg ttatccgtga tggtgttgtc atttttgatg
     8701 gtaaattagc aagtcttaaa cactataaag atgatgttaa agaagtcggc aatgctcaag
     8761 aaggtggtct aatgattgag aattataacg atctcaaaga agatgacact attgaagcat
     8821 acattatgga agaaattaaa cgtaaataac ccctatgaga aacccaactt gtatctttag
     8881 ttggtaggga aatacaaaat tatggctagg ctcgtcctag ctttcaagtt atgagcacaa
     8941 tgaagaaagg attaattatg gctaatcatc gtattgatcg cgttggaatg gaaattaaac
     9001 gtgaagtcaa tgagattttg cgtttccgag ttaatgatcc acgtgtacaa gatgtcacaa
     9061 ttactgatgt tcagatgttg ggtgaccttt caatggctaa agtattttat actattcatt
     9121 cgacacttgc ttctgataat caaaaagcac aaattggttt agaaaaagca actggtacta
     9181 ttaagcgtga acttggcaaa aaccttacta tgtataaaat accagattta caatttgtta
     9241 aagatgaatc tattgaatac ggaaataaga ttgatgaaat gttgcgcaat ctagataaaa
     9301 aagattagaa tcaagattaa gaataataaa aaataaacca tgtcaaaatc atggtttatt
     9361 ttttatagct ttaaagttga gatagctagt attaaggagt tttattgatg atatcaatca
     9421 tttgatactg tgctgtttta gcttcttcaa tagggtaaat tggataaaca tgattcattc
     9481 cctcttgtgc aatatagtta acggggtgat tagctgcttg gagttgtgca gcataatcgc
     9541 gaatatctgg gaagaatatt tctcgtgttc cagtaaataa cgtaatagga gcgagtttag
     9601 tagcaggggc atttttagga ctaacatatg tatggttagt attattagaa ccatttgccc
     9661 aaatttcacc tacacgtgcg agaccccaag cagataggat aggatcggta tcttcgtatt
     9721 tgggaatttc tggatgtttc attgtcacat ctagccatgg agataagagg ataatatttt
     9781 taggttgagg aatagcctct tgccctgatt ggtgagacaa tgcatgcgct aatcctagtg
     9841 ctaatcctcc gccagctgaa tcacccatca aagtgagatt agcgtttttt tcatgaaagt
     9901 gtctataagg gttaaccaat ctagggatag cataatcata ggtataacga ggagtttttg
     9961 gataaattgg taaaataatt tttgcatcaa gactagttga gagcgtctta agcatgctga
    10021 tatggtaagg cgttggatta tttaagtaag agccaccagc taaatagaaa atggtctttt
    10081 gattagcttt accgttgtca ttccaagaat agacttgcat atcaaaaagt ctctcttgaa
    10141 caagccctcc attaacagag tttgggtcta agtaataacc cttatcagat tgttcacggc
    10201 tagcatttaa gtacttttga aatgagcttt catcttgaac tttataataa cttttatcgc
    10261 gctttagcca aaagtattgt tcgataaacc aacttttcca tgaacgccca cttgcttttg
    10321 catgtggtgt acaggcgatg gataaaatgg tgataatact aaatagtagc aatagttttt
    10381 tgaaggacgg tttcaaggta ttctccttaa tataatgtta tttttgtgta atattattat
    10441 aataaaacca acatctttgt caatagaata aagaaagatg agaaatgttt ttcggtttac
    10501 aaatgtagat ttatttgcta aaattggttt acaaacgtaa atcgaaagag gtgagaaaat
    10561 gactatttca aacgcagaat gggaaattat gagagtggtt tgggcacagc agaatacaac
    10621 cagtaatgag atactagcag ttttactaga gaagtatgat tggactcctt caactgtcaa
    10681 aaccttatta agaaggctct tagataaagg ctacgtgagc agagaaaaaa tgggaaaagg
    10741 atttagctat tctcctttaa ttgatgaaga tttagctatg atgtcagagg tagatagtgt
    10801 attgcagaaa gtttgccaga ccaaacatgt tgctattgtt agacacttat tggaaagtat
    10861 accaatgaca gagaaggatc gattaaattt gcaagcatca ctagaagcta agaaaggcaa
    10921 aacacttgaa cgtgtagctt gtaattgcat tcctggtcag tgtcaatgcc attagaaagg
    10981 agaaaatatg gctaatagaa aagaaacatt tttgattgat gggatgactt gtgcatcttg
    11041 tgctttaacg atagaaaaag ctgtcaacaa gttagaccat gtggatagtg cagtagtaaa
    11101 tttagcaaca gagaaaatga cagttacgtt tgatgataca accttatcgc caaacgtgat
    11161 tgaagagtgt gtttctgaga ctggatacga agcatcgctt ttcaaagagg aaacttctaa
    11221 aagccaatct gaacggcacc aattagcaat tgaaaagatg tggcatcgct tttggatgtc
    11281 agcagttgca actatacctc ttctctatat ttccatgggg ccaatgatta acttatggtt
    11341 accatcattt ttgatgccag ataagggacc tctaaactat ggtgtgatac aattattgtt
    11401 aacactaccg gtgatgtatt ttgggcgtat cttttaccaa aatggtttta aagcactttt
    11461 taaaaggcat cccaatatgg attcattagt tgctatagcc acaacagcgg ctttcattta
    11521 tagcttatat ggattgtacg aaatattaca gggtgacata cactacgctc accagcttta
    11581 tttcgaatct gttgcagtta ttttaacact tattactcta ggaaaatatt ttgaaatatt
    11641 atccaaaggg cgtacttcgg cctcaattga aaaattatta actttatcag caaaagaagc
    11701 aagagttatc aaagatggtg aagactatat ggttcctttg gataaagtta aaataggtga
    11761 aacaattctt gtcaaacctg gagaaaaaat tcctcttgat ggccatgttg tagcaggaga
    11821 atcaagtatt gatgaatcca tgttaacagg tgaaagtatt ccagttgaaa aaaaggttgg
    11881 tagtaaggtt tacggtgcca gcattaatgg gcaaggtagc ttaacgattt tcgttgaaaa
    11941 agaagctggc ggttcccttc tatcacaaat tattaactta gtagaagttg cacaggcaag
    12001 taaggctcca attgctaact tagcagacaa agtatcagga gtctttgtcc cttttgttat
    12061 tgtcattgca attttatcag gtttgagttg gtacttaatt ctggggcaaa gttttgcctt
    12121 ctctctgaag attatgattg cagtcttagt gattgcttgt ccatgtgctt tagggttagc
    12181 aactcctaca gctattatgg tggctagtgg taaagcagct gagaatggta ttttatttaa
    12241 aggtggtgaa gtgttggaaa aggctcacca cattgataca atagtttttg ataaaacagg
    12301 aaccttgacc aaagggaaac cagaagtagt tgctatcaaa acatatgggg tagataaaga
    12361 ggagttttta ggtcaagtag catcagtaga aaaactttca aatcatccac ttagccaaac
    12421 aattgttaac aaagcaaaag agaaagaact tcccttaagg gaagtcatgg cttttaaaaa
    12481 tattttgggt tacggtttga gtgcaattat taatggtaaa actatgcttg taggtaatgc
    12541 taacttaatg actaagaacg atgttaattt agatttagca aaggctgata tagaaatagc
    12601 tcaagaagaa gcacaaactg tggtttacgt ttccgaaaat ggagtgttga gcggtttaat
    12661 aactttaacg gaccagctaa agacagatag ccaagaaaca gttaagcagt tacaaagatt
    12721 aggatttaat ttagttttac ttactggtga taataaggct agtgcagatg ccatagcaca
    12781 aaaattgggt atcacgacag ttgtttcaga agttttacct gatcagaaag caaacgttat
    12841 tttagaactt aaagaaaaag gcggacagat tgcgatggtt ggtgatggca tcaatgatgc
    12901 tcctgcgcta gctagcagtg atgtaggtat ctctatgagt tctggaacag atattgctat
    12961 tgaatcagca gatatcgtgt tgatgaaacc tgagttaaca gatctcttaa aagcaatgac
    13021 tatcagtaaa caaacgattc aaattattaa ggaaaattta ttttgggctt tcttttataa
    13081 cgttttagcg attccagtgg caatgggtgt tttacatctt tttggtggtc ccttactcaa
    13141 tcctatgttg gcaggcttag ctatggcgtt tagttcagtt tcagtagttt taaacgcact
    13201 tcgcttaaaa gtcttaaaat aataaagata ataagactga atttttatga agggagaaga
    13261 atatgaaaca tacctatcga gttagtggca tgaaatgtga tggttgtgcc aaaacagttt
    13321 ctgataagtt atcaagtgtg atgggtgttg acgaggttaa tgttgatttg actaaaaatc
    13381 aagttgtcgt aagtggaaaa acattcaaat gtcttttaaa acgttcttta aaagacacaa
    13441 agtattcact agaagaggac ataaattaat aactaaagaa gtggttacag cttttaattt
    13501 cttaatttga gcagtttctc acttcttttt tattctaaaa atgataaact agaatagaat
    13561 tagttgtgag gagtgcagat gtcaatcgat atttggaaca ttttaagttt ggttggaacc
    13621 gtagcttttg caagttcggg agctattgtg gcgattgagg aagaatttga tgttctaggg
    13681 ctttttatct taggttttgt aacggcattt ggtggtggag ctattagaaa tgtactaatt
    13741 ggcttgccta ttgaaacact ttggagtcaa ggaattgctt tttatgcagc agcagcagct
    13801 attttgttta ttatgatttt ccctaacttg ttgagtggta agggacggga tgccgaagtt
    13861 gtatcagatg ctattgggtt agcagcgttt agtgttcaag gagcactata tgcgactcaa
    13921 tctcatcaac ctcttagtgc tgtcattgtc gcagcagtac taactggtgc aggtggtggt
    13981 attgtcagag atgttctggc tggccgaaaa cctggggtac taagaagtga aatatatgca
    14041 ggttggtcaa tattagtggg gatcatttta tattttaaaa ttgctaaaac aacaactgac
    14101 tattatttgt tagtgttagt tgtaactagc ctaaggatgt tggggtataa gaaacaatgg
    14161 cacttgccag ttgtcagacc acgtaagaaa tgatagagga gaaagagatg ataaaattaa
    14221 ttgctattga tatggatgga acgcttttaa atgatgaaaa gaaaattcca aaggaaaata
    14281 tccaagctat taaagaagca actcaagcag gtattaaaat tgtactgtgt actgggcgac
    14341 cgatgtcagg tatacttcct tattttaatg aactaggttt aacaaaagag gagtatatta
    14401 tcatgaataa tggttgtagc acttatagca cgaaagattg gcaattgatt gattctgcaa
    14461 ctttaacaca tgatgaactc atctttcttg aggaggttgt taaagaattt cccaacgttt
    14521 gtttaacgtt aacagctgaa aatacatttt acgctgttgg tgaggaagtt cctgaaattg
    14581 ttgcttatga tgctgattta gtttttacaa aagcaaagtc aataagttta gatgctcttc
    14641 gcaatcaaga agaaattgtc tttcaagcaa tgtacatggg attagatgca gatgtaactg
    14701 cttttcaaga agctgttgaa gaagccttaa tctcgaaatt tagtggagtt cgaagccaag
    14761 actatattta tgagattatg cctcaaggag taacaaaagc aagaggctta aaatccttga
    14821 tagcaaaatt aggactggat attaatcaag ttatggctat tggtgatgct cctaacgata
    14881 ttgaattatt agatttagtt cccaatagcg ttgctatggg gaatgcatca gatgagatta
    14941 aatctagatg taaatatata acggtggata ataaccaagc cggggttgct aaagctattt
    15001 atgactatgc tttacgttaa aatagatata gaataataaa aggaacttta aacactgttt
    15061 gtttagggtt cttttgttta ataaactaac aaaattcaga agtttacaga tagcttatgc
    15121 tataatagac ctatgacaaa caagaataaa ttacttttaa ttgatggctc atctgtagcc
    15181 tttcgtgctt tttttgctct ttataatcaa attgaccgct ttaaaaacaa ttctggactt
    15241 catactaatg ccatatatgg tttccatttg atgttgaatc atattttagg tcgtgtccag
    15301 cctagtcata ttttagtggc ttttgatgct ggaaaaacta ctttccgtac ggaaatgtat
    15361 gcagattata aaggtgggcg tgctaaaact ccggatgaat ttcgtgaaca atttccttat
    15421 attcgtcaac aattagatgt cttaggcatc aaacattatg aattggaaca ttatgaagcc
    15481 gatgatatca ttggtactct tgcaaaacaa gccgaagcta gtaaagagca ttttgatata
    15541 acggtggttt ctggggataa agatttaatt caattaacag acactaacac ggtagtagaa
    15601 atctctaaaa agggagttgc tgagtttgaa gaattcacgc cagcttacct tatggaaaaa
    15661 atgggaatta cgccgagtca atttattgat ttaaaagctt tgatgggaga taaatcagat
    15721 aatattcctg gggttactaa aatcggtgaa aaaactggct tgaagttgct atcagaatat
    15781 ggttccttag aagggatcta tgaaaatatt gaggctatga aacagtctaa gatgaaagaa
    15841 aaccttatca atgataagga gcaagctttc ttatcaaaga ccctagctac aatcaacata
    15901 gcatcaccta taacaatcgg cttagaagat atcctttata gtggaccgca agatataaaa
    15961 gccttgtctc aattttatga tgagatggat tttaaacagt ttaaagcagc tctgggtgag
    16021 gagacaagtc aggaagattt tgaggttgac tttactgaag tggagcaatt aaaaccagaa
    16081 atgttctccg ataatgattt ttattacttt gagatgttag gtgataacta ccacgtagaa
    16141 gatttaattg ggatagcttg gggaaatagc gatacaatct acgctactag tgatgtatcg
    16201 ttattgcaag aagctttatt taaaaaagct ttatcaaaac ctatcaaaac ttatgatttt
    16261 aaaagaagta aggtcctttt aaatcgtttt aatattgatt taccagagcc agcctttgat
    16321 actagattgg ccaagtacct tttgtctact actgaggata atctagtatc aacaattgct
    16381 aggttatata caaatctgcc actggatact gatgatgctg tttatgggaa aggtgctaaa
    16441 cgcgctatac cagaaaaaac acgatttctt gagcatctag ctaaaaaagt aaaagtttta
    16501 gtggattctg aggctaatat tatgcaacag cttaaagcta atgagcaaga agaattgctg
    16561 tttgaaatgg agcaaccttt agcgaatgtt ttagctaaaa tggaaattcg aggtattaag
    16621 gttaagaaaa ataccttgaa tgaaatggct attgaaaatc aaaaagtcat cgaaacctta
    16681 acacaagaga tctatgaatt ggctgggcaa gagtttaata ttaattctcc aaaacagtta
    16741 ggaaagttgt tgtttgaaac gttaggtctt cctgtagaaa tgactaaaaa aacaaaaaca
    16801 ggttattcaa ctgcagtaga tgtattagag cgcttagctc cgatttctcc tcttgtaact
    16861 aaaattttag agtaccgtca aatcactaaa ttgcaatcaa cctatattat tggtttacaa
    16921 gattatattt tagaagatgg taaaattcat acacgttatg ttcaagattt gactcagaca
    16981 ggtcgtcttt caagttctga cccaaacctt caaaatatac cagttagact agagcaagga
    17041 cggcttatcc gaaaggcttt tgttccatca gaagacaatg ctgttttatt gagttcagat
    17101 tattctcaaa tagaattgcg cgtgttagca catatttcaa aagacgaaca tttgatcgct
    17161 gcctttaaag agggagcaga tattcatact tcaacagcta tgcgtgtttt tggtatcgaa
    17221 aaacctgaga atgtcactcc caatgatcgt cgaaatgcta aggctgttaa ctttggaatt
    17281 gtttatggta tttcagattt tggtctatcg cataatttag gaatacctcg taaactagcg
    17341 aagcaatata ttgacaccta ttttgagcgt tatcctggaa taaaaaacta tatggaaaca
    17401 gttgttcgtg aagcaaaaga taagggttac gttgaaacac ttttccatcg tcgtcgttca
    17461 ctaccagata ttaattctcg taattttaac attcgccaat ttgcagagcg cacagcaatc
    17521 aattcaccaa tccaaggaag tgcagctgat attcttaaaa ttgcaatgat aaatttagat
    17581 agggtacttt ataaaggtgg ttataaatca aaaatgctgt tgcaagttca tgatgaaatt
    17641 gtacttgaag tccctaacga ggagatagga gcaatcaagg agttggttac aaaaacaatg
    17701 gagtcagcaa ttagtttatc ggtacctttg attgcagatg agaacgcagg tgagacttgg
    17761 tatgaagcga agtagtggac aacatacgta aaggagtcat taatatggtt tatcattttc
    17821 aaaatccaag tgattttatg ttaaaaaatt atttaacaaa agcaaagacc atcgcagttg
    17881 taggattgtc agaccgtcaa gaaactgcag cttatcaagt atcaaaaata atgcaagaag
    17941 caggttatca aattattcca gttaatccta aaaatgcagg acagaaaata cttgggcaaa
    18001 tgacatatgc ctcacttaag gatgtcactg aacatattga tattgttaat atttttcgac
    18061 gtagcgagta tttacctgat attgcaagag aatttttaga agtagacgct gatattttct
    18121 gggctcagct tggacttgaa agtcaagaag cagagacaat tctaaagcaa gcaggacaca
    18181 aacagattgt catgaataaa tgtctgaaag ttgaatacca aaaattacac tcagaaacta
    18241 actaattacc gtgtttaata ggcaaataat ataaattatg ctataataac catgctaaat
    18301 taaagttcgt gctggaaagg agtcctatgg acagtcattc acatggtcat agacctctcg
    18361 atgcttatga aaacgtccta gagcatctcc gtgaaaaacg tattcgaatt actgagactc
    18421 gaaaagctat tatttcctat atggttaata gtcgtgagca tccgagtgca gaaaagattt
    18481 acaatgattt attaccagaa tatcctaaca tgagcttggc aacagtttac aataacctta
    18541 aggtccttgt ggatgagggg tttgtgacag agctaaaact ttgcaattat agtacaactt
    18601 attatgattt tatggggcat cagcacctaa acattgcctg tgaagattgt gggaaaatcg
    18661 ttgattttgt cgatgttgac ttattggata tttctagaga agcacaccaa caaacaggtt
    18721 ttgaagtgac tagagttcag ttagtagctt atggtatctg tccggagtgt cagaggaagc
    18781 gtaaggataa atataaaaaa gattgaaacg taattgttca atcttttttt gtttcta
//
LOCUS       DBJYSH010000034        18711 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.034, whole genome shotgun sequence.
ACCESSION   DBJYSH010000034 DBJYSH010000000
VERSION     DBJYSH010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 18711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18711
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.034"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            49..1857
                     /gene="dnaG"
                     /locus_tag="ACUBV3_000900"
     CDS             49..1857
                     /gene="dnaG"
                     /locus_tag="ACUBV3_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HHU3847413.1"
                     /translation="MGYFCGGHDLAIDKEKISEIKNSVNIVDVIGEVVGLTKTGRNHL
                     GLCPFHKEKTPSFNVIEDRQFFHCFGCGRSGDVFKFVEDYQHISFLDSVQVLAERSGI
                     PLDTNFKGQVPKKPKANQSLLDIHRVASGFYHAYLMTTNDGERARQYLAERGVTEDLI
                     KHFQIGLSPGGQDFLYRRLAKEFDEKTLMSSGLFNYSENSNQFYDSFNNRIMFPLTND
                     IGEVIAFSGRVWTQEDIDRKQAKYKNSRATPIFNKSYELYHLDKARAVINKAHEVYLM
                     EGFMDVIAAYRAGIENVVASMGTALTNEHVRHLKRFTKKVVLTYDGDRAGQNAIDKSL
                     ELLSDMTVDIVRIPNKMDPDEFLQANSAEDFKQLLENGRISNTEFYIHYLKPENTDNL
                     QSEIAYVEKIAKLIAKSPSITAQNSYITKVAELLPDFDYFQVEQSVNNERLHHRSQQQ
                     ASSSVQTSATVQLPQTGKLSAITKTEMQLFHRLLNHPYLLNEFRNRDNFYFDTTEIQV
                     LYELLKESGEITSYDLSQESDKVNRTYYIILEEQLPVEVSIGEIEAVEKARDRLLKER
                     DLRKQSQLIRQSSNQGDEEGALAALENLIAQKRNME"
     gene            1865..2974
                     /gene="rpoD"
                     /locus_tag="ACUBV3_000901"
     CDS             1865..2974
                     /gene="rpoD"
                     /locus_tag="ACUBV3_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019787874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HHU3847414.1"
                     /translation="MAEKKGNTTFNVQVADFIRNHKKQGTAIDDEVTEKLVIPFVLDA
                     DQIDDLLERLTDGGISITDKEGNPSTKYVVEGPKPEELTDEELIGSNSAKVNDPVRMY
                     LKEIGVVPLLTNEEEKELAVAVAEGDLMAKQRLAEANLRLVVSIAKRYVGRGMQFLDL
                     IQEGNMGLMKAVDKFDYSKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINK
                     LVREQRNLLQELGQDPTPEQIAERMDMTPDKVREILKIAQEPVSLETPIGEEDDSHLG
                     DFIEDEVIENPVDYTTRVVLREQLDEVLDTLTDREENVLRLRFGLDDGKMRTLEDVGK
                     VFNVTRERIRQIEAKALRKLRHPSRSKQLKDFMED"
     gene            3083..3424
                     /locus_tag="ACUBV3_000902"
     CDS             3083..3424
                     /locus_tag="ACUBV3_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017766829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HHU3847415.1"
                     /translation="MSEVKQYSEEEVGKIKDRILEALEMVIDPELGIDIVNLGLIYEI
                     RFEDNGRTEIDMTLTTMGCPLADLLTDQIHDVMKTVPEVTETEVKLVWYPAWSVDKMS
                     RYARIALGIRS"
     gene            3514..4368
                     /gene="rfbD"
                     /locus_tag="ACUBV3_000903"
     CDS             3514..4368
                     /gene="rfbD"
                     /locus_tag="ACUBV3_000903"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HHU3847416.1"
                     /translation="MILITGANGQLGSELRHLLDERTQEYVAVDVAEMDITNAEMVDK
                     VFEEVKPSLVYHCAAYTAVDAAEDEGKELDFAINVTGTENVAKAAAKHDATLVYISTD
                     YVFDGEKPVGQEWEVDDLPDPKTEYGRTKRMGEELVEKYTSKFYTIRTAWVFGNYGKN
                     FVFTMQNLAKTHKTLTVVNDQHGRPTWTRTLAEFMTYLAENQKDFGYYHLSNDAKEDT
                     TWYDFAVEILKDTDVEVKPVDSSQFPAKAKRPLNSTMSLEKAKATGFVIPTWQDALKE
                     FYKQEVKK"
     gene            4485..5639
                     /gene="cps2T"
                     /locus_tag="ACUBV3_000904"
                     /gene_synonym="wchF"
     CDS             4485..5639
                     /gene="cps2T"
                     /locus_tag="ACUBV3_000904"
                     /gene_synonym="wchF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001154391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta 1-4 rhamnosyltransferase Cps2T"
                     /protein_id="HHU3847417.1"
                     /translation="MQDVFIIGSRGLPARYGGFETFVSELINHQKSSDIKYHVACLSD
                     KEHHTHFNFADADCFTINPPQLGPARVIAYDIMAINYALDLVKTHDLKEPIFYILGNT
                     IGAFIWHFANKIHKVGGLLYVNPDGLEWKRSKWSRPTQRYLKYAEKCMTKNADLIISD
                     NIGIENYIQSTYSNVKTRFIAYGTEINSRKLSSDDPRVKQLFKKWNIKSKGYYLIVGR
                     FVPENNYETAIREFMASDTKRDLVIICNHQNNPYFEKLSLKTNLQQDKRVKFVGTLYE
                     KDLLDYVRQQAFAYIHGHEVGGTNPGLLEALANTDLNLVLDVDFNKSVAGLSSFYWTK
                     KEGDLAKLINDSDQQQDLSTYGDRAKAIIQENYTWKKIVEEYEDLFLNES"
     gene            5629..6570
                     /locus_tag="ACUBV3_000905"
     CDS             5629..6570
                     /locus_tag="ACUBV3_000905"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HHU3847418.1"
                     /translation="MKVNILMATYNGEKFLAQQIESIQKQTFKEWNLLIRDDGSSDKT
                     CDIIRNFTAKDSRIRFINENEHHNLGVIKSFFTLVNYEVADFYFFSDQDDVWLPEKLS
                     VSLEAAKHKASDVPLLVYTDLKVVNQELNILQDSMIRAQSHHANTTLLPELTENTVTG
                     GTMMINHALAEKWFTPNDILMHDWFLALLAASLGEIIYLDLPTQLYRQHDNNVLGART
                     MDKRFKILREGPKSIFTRYWKLIHDSQKQASLIVDKYGDIMTANDLELIKCFIKIDKQ
                     PFMTRLRWLWKYGYSKNQFKHQVVFKWLIATNYYNKR"
     gene            6572..7303
                     /locus_tag="ACUBV3_000906"
     CDS             6572..7303
                     /locus_tag="ACUBV3_000906"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HHU3847419.1"
                     /translation="MMKKILLVIPAYNEEGSIAKTVQTIVDFKASRSLPFELDYIVIN
                     DGSTDGTPELLDRLGLNHIDLVQNLGIGGCVQTGYLYANRNHYDVAVQFDGDGQHDIR
                     SIEDVVMPILNDEADFVIGSRFVDKKHQNFQSTAMRRLGINLISAAIKLTTGHKVYDT
                     TSGYRAANAALIAYLSCHYPVQYPEPESTARILKKGYRLKEVTANMFEREAGTSSISS
                     LKSIFYMTDVLTSIIIAGFIKEDDK"
     gene            7300..7653
                     /locus_tag="ACUBV3_000907"
     CDS             7300..7653
                     /locus_tag="ACUBV3_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000050393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2304 domain-containing protein"
                     /protein_id="HHU3847420.1"
                     /translation="MSLALQIEMFILAIVLLYMIIRMVNKNTFSVKRSLPWLLVGLIL
                     VFISVFPGVVSFIAHHLGFALTMNFLLFGAILFLFCLELFDTSSNTKRDEQIKRLIQE
                     VSLLKKEIEDSTLDE"
     gene            7646..9379
                     /locus_tag="ACUBV3_000908"
     CDS             7646..9379
                     /locus_tag="ACUBV3_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HHU3847421.1"
                     /translation="MSKKHIFICLFFMLFLVGCQNNKKVKTDKKVDITAYYYHNNTPQ
                     YSVQINAIHLQTALDKVRTGEFESKDFTHYTFDSIKRKYQVSGKKVLMDNNYRFGSGI
                     KNTRQDAIAIVRLLLEKNKDYLIYMNGLEEPSVLYNPENKRYKFTNNKGKAIGNIPVG
                     LTAFENSAETQEYVLKNIQKNETIYLGNTRVDNPRVTVNNKKRDTIGVEYGKRITYRI
                     PIYSKQLTVRVSPNFVVDSTNYNYRLSQAPIIAEEGKEGRLYPIDSSISVDGDKIVLN
                     SSGDETEQKELLQNSLYKLHSIDKLDFDLTNADIKELEITGHVASKRDYNLSVAQEKK
                     GVIETKRSLISVDNQKQDQGIFVEDSKGYIKTPSLSSYGINFAMYDDNQNRLLQGSEY
                     ILGRLNADKKVSLYQPNGFWINTQKTIKSLNKEYLNQNAYHIRGNNIIGIDGYSNDID
                     LNPRLWAYNYKKQVKSNQSLFKLRGLSNKYKYFLIQTKSPKGYLQSNKPFYFTVNKDS
                     VSKAQFGNYNINGYIMDMQYNKQEYNALANTPKGQKTKKTFKPMTLVILFAVALFIFY
                     ITAIRFVFKRM"
     gene            9404..10228
                     /locus_tag="ACUBV3_000909"
     CDS             9404..10228
                     /locus_tag="ACUBV3_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LicD family protein"
                     /protein_id="HHU3847422.1"
                     /translation="MKEMTVSEIREVQLEMLAYIDKVARDNKIEYSLGGGSLLGAMRH
                     KGFIPWDDDIDLMLERSQYERLMKALADANNSDFKLLHHSVEKNLWPFAKLYHTKSMY
                     LSKTDRIHPWTGIFIDIFPLDRLPESAEERQRFFKKVHSAAANLMCTTYPNFASGSRK
                     LYANARLILGLPRFIAYHGQAKKRAEIVDQVMETYNNQEVPYMGYTDSRYRLKEYFPR
                     EIFSEYEDVMFENIKTRKIKNEHAYLNQLYGGSYMELPPESKRESHSYYTWYWKEG"
     gene            10228..10950
                     /locus_tag="ACUBV3_000910"
     CDS             10228..10950
                     /locus_tag="ACUBV3_000910"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IspD/TarI family cytidylyltransferase"
                     /protein_id="HHU3847423.1"
                     /translation="MMNIGVIFAGGVGRRMNTKGKPKQFLEVHGKPIIVHTIDIFQNT
                     EAIDAVVVVCVSDWLDYMNNLVERFNLTKVKAVVAGGETGQMSIFKGLEAAEQLATDD
                     AVVLIHDGVRPLINEEVINANIQSVKETGSAVTSVRAKETVVLVNDSSKISEVVDRTR
                     SFIAKAPQSFYLSDILSVERDAISKGITDAIDSSTLMGMYNRELTIVEGPYENIKITT
                     PDDFYMFKALYDARENEQIYGM"
     gene            10950..12008
                     /locus_tag="ACUBV3_000911"
     CDS             10950..12008
                     /locus_tag="ACUBV3_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HHU3847424.1"
                     /translation="MMIKFSNKIVQEDLALISNQNYINWERIANKGVLISGSNSMLAS
                     YMVFLLAYLNETRNYQTQIIATARNIEKARDKFSDLVGKDYFTLIPYDVEERLEYDGK
                     VDYIIHAASNASPTAILSNPVSIIKANTIGTLNLLDFAKEKTIENFLFLSTREVYGTS
                     IKEVIDEEAYGGFDILATRACYPESKRMAETLLQSYYDQYKVPFTIARIAHSFGPGME
                     LGNDGRIMNDLLSNVIDGKDIVLKSSGTAERAFCYLADAVSGLFTILLNGEVGQAYNV
                     ANEDQPIMIKDLAQKLVDLFSDKNISVVFDIPKTMSAGYSKMGRTRLTMAKLEALGWK
                     REVSLESGILKTVQAFEE"
     gene            12010..12945
                     /locus_tag="ACUBV3_000912"
     CDS             12010..12945
                     /locus_tag="ACUBV3_000912"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HHU3847425.1"
                     /translation="MVKTAVLMATYNGEKFISEQLDSIRQQTLKPDYVLLRDDCSTDE
                     TVNVVNNYIAKHELEGWKIVKNDKNLGWRLNFRQLLIDVLAYEVDYVFFSDQDDIWYL
                     DKNERQFAIMSDNPQIEVLSADVDIKTMSTEASVPHFLTFSSSDRISQYPKVYDYQTF
                     RPGWTIAMKRDFAQAIADNWTFGANISHDNLIETTASIIGNGYNLNESVGVHIRYGGN
                     ASGNSKFVLNWKSPKTQHITGLKTFVDFYETMQKVVNKYQNDVALNQRLAFEKERLEA
                     SKLSLIHNSAFVIKNWRLYNSNSGRMRDLLFAFKK"
     gene            12954..13901
                     /locus_tag="ACUBV3_000913"
     CDS             12954..13901
                     /locus_tag="ACUBV3_000913"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HHU3847426.1"
                     /translation="MVKTAVLMATYNGQGFIHDQLDSIRNQTLRPDYVLMRDDGSTDD
                     TVKVVEDYIKEHRLDGWSITSNDKNLGWRLNFRQLLIDVLAYEVDYVFFSDQDDTWYH
                     HKNKMQVDIMEERQDINLLSADIDIKKLSEEATIPNNFKFNGEETISKYPLDFSYHNY
                     RQGWTFCLRKSFVDQVMTFYSDNLILSHDNLFAGVSGVIGSGYNLNRSVGEHKRHAEN
                     ASGNILNMSSSYERHLQELYFVLSYYIILVPFLEKSSSSQLREAKAYLDFNQQRYKNA
                     QNKDFVAILKQMMTKKRFYDSFSNRIRDLVFLLKDIKRS"
     gene            13903..15267
                     /locus_tag="ACUBV3_000914"
     CDS             13903..15267
                     /locus_tag="ACUBV3_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2142 domain-containing protein"
                     /protein_id="HHU3847427.1"
                     /translation="MNLKKVRLFEKFFLDKNLHKTYFILGLTITIVLSLLMPFFNEPD
                     GQYHLAVSGRISNNIIDTSYYGEYNIDSGMRRQSTSYRDGTRFEKYYLNKANFVDGNH
                     LPREINYSKHNFVFWGHLVPAVGLFLGKFVYPSMGVMITVARLFSSFVSITLVALIIR
                     YVKKGKLIYFAIFLSPVVLNSFASLSYDSTGYIVFGAFLSLLINLLVDKSLTKNRSIA
                     LIGLILLVIVACKQNYWLFLLLVIPVLLQVNNPKLMGYNQKLSHLFQGLKSKKLFWLC
                     LTFLSLAIVYVLTARHGGVLAVLRRYVMTFGYNYAGINILSNDITSWLASPYPSQNFI
                     PTWVSSVWYLMVFLVWFSEEKFVKSKNMGFLFLASFFVGILGVFFIMLEYRGAPTSYI
                     EGVQGRYFTVTLLLVQVFASSIPARLNEAGRKVVPYALTGLVIVSNALLLFDTVISLI
                     MRRN"
     gene            15267..16691
                     /locus_tag="ACUBV3_000915"
     CDS             15267..16691
                     /locus_tag="ACUBV3_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HHU3847428.1"
                     /translation="MKLLKNMFYNTSYQLLTLLLPLVTVPYVSRVLSPQGIGINAYTS
                     SIVMYFTLFGALGISLYGNREIAFVQSNKYKRSKIFWELVVLKLASVSIATLLFFGFV
                     LLTNEWQLFYLIQGINLLATATDISWYFIGVEDFKIIVIRNTIVKLITVVLTFLVVKT
                     PDDLALYMFLIAFASLLGNLTVWHHLKHEIIKIPFSRLDILIHLRPTLMLFLPQITMQ
                     IYLSLNKSMLGAMDSVVSAGYFDQSDKIIRILFTIVSAIGGVFLPRLSSLFSSGKEKQ
                     AKALLLKLVDLSNAISMLMIAGVVGVSSTFAVFFFGKGYEAVGPLMAVESLMIICISY
                     GNALGTQYLLASRRTKAYTMSAVIGLVANVVLNILLIPILGAMGAIISTVITEFIVSL
                     YQAISLRDVFTFKELTRGMLRYLIAATLSGAVLYYINTQMSVSLVNYVIQSLVAVTIY
                     VGIVFITKAPVIQLFRDFRKEHRT"
     gene            16688..17536
                     /locus_tag="ACUBV3_000916"
     CDS             16688..17536
                     /locus_tag="ACUBV3_000916"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HHU3847429.1"
                     /translation="MKYLAGIVTFNPNIERLDQNIRAIYPQVSHIYIVDNGSKNKEEI
                     SQLVADYNEEGHLTVDYLTENKGIAYALNCIGQFAVAQEFDWFLTLDQDSVVLGDLID
                     NYENYLLLPKVGMLSCLYQDMNREDLVMQEFDYKEIEECITSAALMKTSVFEETSGFA
                     EEMFIDFVDSEMNYRLSEMGYKTYQVNFIGLLHEIGHSSRVKKFGHVFHVLNHSPFRK
                     YYMIRNAIYIIKKYGKKKRHKYLVFMRNEFVRVLVAEEQKSKKLVAMIKGLKDGLLMK
                     VDKYGK"
     gene            17526..18674
                     /locus_tag="ACUBV3_000917"
     CDS             17526..18674
                     /locus_tag="ACUBV3_000917"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HHU3847430.1"
                     /translation="MENKVKTVAVFSGYYLPFLGGIERYTDKMTADLVKRGYRVVIVT
                     TNHDDLPIIDEDKGRKIYRLPTKNIVKQRYPIINKNREYNTLMKHVSDENIDFVICNT
                     RFQLTTLEGLSFAKKHRLPSIVLDHGSSHFSVNNRFLDFFGAIYEHLLTARVKHYRPD
                     FYAVSKRSVEWLKHFNIEAKGVIYNSVSESLGSDFADTAYLEKSADDIFITYAGRIIK
                     EKGIELLLEAFSMSQYSENVYLQIAGDGPELAHLKEKYQSKQINFLGKLNFEQTMSLM
                     AQTDIFVYPSMYPEGLPTSILEAGLLSSAIIATDRGGTVEVIDSPELGIIMEENTQSL
                     HESLDLLVKDKALREKLQQNIAKRIKEHFTWEKTVEKLDYIIQKELNL"
BASE COUNT         6252 a         2737 c         3652 g         6070 t
ORIGIN      
        1 aaatttacac agaaaagaaa aaatgatata atcaaatgta aaactataat gggttatttc
       61 tgtggaggtc atgacttggc aattgataaa gaaaaaattt ctgaaatcaa gaacagtgtt
      121 aacattgttg atgtcatcgg tgaagttgtc ggtttgacaa aaacaggacg caatcatctt
      181 ggtttatgcc catttcataa agaaaaaacg ccatctttta atgttatcga agataggcag
      241 ttttttcatt gttttggttg tggtcgctct ggtgatgtct ttaaatttgt ggaagattac
      301 caacatatta gttttttaga tagtgtacaa gttctagctg aacgttcagg aatacctcta
      361 gatactaatt ttaaggggca agttcctaaa aaacctaaag ctaatcagtc gcttttagat
      421 attcatagag ttgcttcggg attttaccat gcctatttaa tgacaactaa tgatggtgaa
      481 cgcgctagac aataccttgc cgagcgcgga gtgacagagg acttaattaa acattttcaa
      541 ataggattat cgccgggagg gcaggatttt ctatatcgtc gtctggcaaa agagtttgat
      601 gaaaaaactt tgatgtcttc tggtttgttc aattattcag aaaatagcaa tcagttttac
      661 gattcattta ataatagaat tatgtttccg ttaactaatg atattggtga agtcattgcg
      721 ttttcaggac gagtttggac tcaagaggac attgatagaa aacaagctaa atataaaaat
      781 tctcgtgcca cccctatttt taataagtct tacgagcttt accatttaga taaagcgcgt
      841 gctgtaatta ataaagcaca cgaagtttac ctaatggaag gctttatgga tgttattgca
      901 gcttatcgtg caggaattga gaatgttgtt gcttctatgg ggaccgcttt aaccaatgaa
      961 cacgttagac accttaaaag atttactaag aaagttgttc tgacttatga tggtgataga
     1021 gctggtcaaa acgcaattga caagtcttta gaactgctat cagatatgac tgttgatatc
     1081 gttaggatac ctaataaaat ggaccctgat gaatttctac aagcaaattc agcagaagat
     1141 tttaaacaac tcttagaaaa tgggcgcatc agtaatacag agttttatat ccactactta
     1201 aagcctgaaa acaccgataa tttacaatct gaaattgctt atgttgaaaa aatagcgaaa
     1261 ttaattgcta aatctccatc gataacagca caaaattcat atattacaaa agtggctgaa
     1321 ctcttgccag attttgatta ttttcaagta gaacaatcgg ttaataacga acgattgcat
     1381 catagaagtc aacagcaagc ttcatcttca gtacaaacaa gtgctacagt gcaattacca
     1441 cagacaggta agttatcagc tatcactaaa acagaaatgc aattgtttca tcgattattg
     1501 aatcatccct acctgttaaa tgaattccgt aatagggata atttttattt tgatacaaca
     1561 gaaatacaag ttttatatga gttgctaaaa gaaagtggtg agatcacgtc ttatgattta
     1621 tcacaagaaa gtgacaaagt taatcgtact tattatataa ttttagagga acaattacct
     1681 gtagaagtat ctattggaga gattgaagcc gtcgaaaagg cacgtgaccg tttattgaaa
     1741 gaacgtgatt taagaaaaca aagccagtta attagacaat ctagtaatca aggtgatgaa
     1801 gaaggagcct tggctgcatt agaaaattta attgctcaaa aaagaaatat ggaatgagga
     1861 acctatggca gagaaaaaag gaaatacaac ttttaatgtt caagttgcgg attttattcg
     1921 caatcataaa aaacaaggta ctgcaattga tgatgaggta acagaaaaac ttgttattcc
     1981 ttttgtttta gatgcagatc aaattgatga tttgttagaa cgattgacag acggtggcat
     2041 ttcaatcaca gataaagaag gcaatccttc aacaaagtat gtggtagaag ggccaaaacc
     2101 tgaagaattg actgatgaag agctcatagg aagtaattct gctaaagtca atgacccagt
     2161 acgtatgtac ttgaaggaaa ttggtgttgt tcctctttta acgaatgaag aagaaaaaga
     2221 attggctgtt gcggttgctg agggagattt aatggctaaa caacgtttag ctgaagcaaa
     2281 ccttcgtctt gttgtctcaa ttgcaaaacg ttatgttgga cgtggaatgc aatttcttga
     2341 tttaattcaa gagggtaaca tggggctcat gaaagctgtt gataaatttg attattcaaa
     2401 gggattcaaa ttttcaactt atgcaacatg gtggattcgt caagctataa cacgtgcaat
     2461 tgcagatcaa gcacgtacga ttcgtattcc tgttcatatg gttgaaacga ttaataaatt
     2521 ggtccgtgaa caacgtaatc ttcttcaaga acttggtcaa gatccaactc ctgagcaaat
     2581 cgcagaacgc atggatatga caccagataa agtacgtgaa atcctaaaaa ttgctcaaga
     2641 gccggtatcg ctagaaacgc caatcggtga ggaagatgat agccatttgg gtgatttcat
     2701 tgaagacgaa gttattgaaa atccagtaga ttatactaca cgtgttgttc ttcgtgagca
     2761 attagatgag gttttagata ctcttactga ccgtgaagag aatgttcttc gtttacgctt
     2821 tggtcttgac gatggtaaaa tgcgtacgtt agaggacgta ggtaaagtat ttaatgttac
     2881 tcgtgagcgc attcgtcaga ttgaagctaa agcccttaga aaacttcgcc atcctagtcg
     2941 tagcaagcaa cttaaagatt tcatggaaga ttgattgctt ctcgataatc gtttttagaa
     3001 taggaagttt agtttctaat aaaaatgatt tgcaaaggag ttatcttaaa gacggtatta
     3061 atattttcag agaggtatta taatgtcaga agtaaagcaa tatagcgaag aagaagttgg
     3121 aaaaataaaa gatcgtatcc tagaagcttt ggagatggtt atcgatcctg aattgggaat
     3181 agatattgtt aaccttgggt taatttatga gattcgcttt gaagataacg gtcgaactga
     3241 aattgatatg actttaacga ctatgggatg cccgttagca gatttattga cagatcaaat
     3301 tcatgatgtt atgaaaacag tcccagaggt aactgaaacc gaggtgaaat tggtatggta
     3361 cccagcctgg tctgttgata agatgagtcg ttatgctcgt atcgctttgg gaattcgttc
     3421 ataagataaa gagtaaaggg atgaaatatt ccctttttta gtgtacaata aacgaagaat
     3481 taaaagttaa acttattttt aaaggagcaa ggaatgattt taattacagg ggcaaacggg
     3541 caattaggta gtgaattacg tcacctttta gacgaacgaa ctcaagagta tgttgctgtt
     3601 gatgtagctg aaatggatat tacaaatgcc gagatggtgg acaaagtctt tgaagaggtg
     3661 aagccatctt tagtttacca ttgtgcagct tacacagctg ttgatgcggc agaagatgaa
     3721 ggaaaagagc tagactttgc aatcaatgtc actggtacag agaatgtcgc taaggctgct
     3781 gctaaacatg atgcaacact tgtttacatt tcgacggact acgtgtttga tggtgaaaaa
     3841 ccagtggggc aagaatggga agtagatgat ctgcctgatc ccaaaacaga gtacggtcgt
     3901 acaaaacgta tgggtgaaga gttagttgaa aaatatactt caaaattcta taccattcgc
     3961 acagcgtggg tatttggtaa ctatggtaaa aactttgtct ttacgatgca aaaccttgca
     4021 aaaacacaca aaactctaac tgttgttaac gaccaacatg gacgaccaac atggacacgt
     4081 actttggctg agtttatgac ttatttagct gaaaatcaaa aagattttgg ttactatcat
     4141 ttatcaaatg atgctaaaga agatacaaca tggtatgatt ttgcggttga aatcttaaaa
     4201 gacacagatg tggaggttaa gcctgttgat tctagtcaat tcccagcaaa agcaaaacgt
     4261 cctttaaatt caactatgag tttggaaaaa gcgaaagcaa caggatttgt gattcctact
     4321 tggcaagatg ctttaaaaga gttctacaag caggaagtta aaaaataaga tcaaaacaaa
     4381 gagtagtaag gctagagaat ccttttagct tactgctctt tttagctaac ttttctaatt
     4441 tatggtataa ttgtatggat tgtttagcta gaatggagaa gatgatgcaa gatgttttca
     4501 ttataggaag tagagggttg ccagctcgtt acggtggttt tgaaactttt gtttcagaat
     4561 tgattaatca tcaaaaaagt tccgacataa aataccatgt tgcatgcctt agtgataaag
     4621 aacatcatac tcattttaac tttgctgacg ctgattgttt tactataaat cctccccaat
     4681 tagggccagc acgtgtgatt gcttatgata ttatggccat taattatgcc cttgacttgg
     4741 ttaagacaca tgatttaaaa gagcctattt tttatatttt aggaaataca attggtgcct
     4801 ttatttggca ttttgccaat aaaatacata aagtcggtgg cttattgtat gttaatccgg
     4861 atggtttaga gtggaagcga tcaaagtggt ctcgtcccac acagcgttat ttaaaatacg
     4921 ccgaaaaatg tatgactaaa aatgcagacc taattatttc tgataatatt ggtattgaaa
     4981 attacattca atctacctac tctaatgtga agacaaggtt cattgcttac ggtacagaga
     5041 ttaattctag gaaattatcg tcagatgatc cacgtgtcaa acagttgttt aaaaaatgga
     5101 atattaagtc taagggttac tatctaatcg ttggtcgatt tgtccctgaa aacaattatg
     5161 aaacggctat tagggagttc atggcttcag atactaagcg tgatttagtt attatctgta
     5221 accatcaaaa taacccctac tttgaaaagt tgtccttaaa gacaaacctt caacaagata
     5281 aaagagttaa gtttgtaggt acgctctatg aaaaagatct gctggattat gttcgtcaac
     5341 aagcctttgc ttatattcat gggcatgaag ttggcggtac taatccagga ctgcttgagg
     5401 ctttagctaa tactgatttg aatcttgttc tagatgttga tttcaacaaa tcagtagcag
     5461 gtctctcaag tttttactgg actaaaaaag agggggattt agctaagctt attaacgata
     5521 gtgatcaaca acaggattta tctacttacg gtgatcgagc aaaagccatt attcaagaaa
     5581 actatacttg gaaaaaaatt gttgaagaat atgaggattt gtttttaaat gaaagttaat
     5641 attttaatgg ccacctacaa tggagagaag tttctggcgc agcaaatcga aagtatccaa
     5701 aagcaaacct ttaaagagtg gaacctccta atcagagatg atggttcaag tgacaagaca
     5761 tgtgatatta ttagaaactt tactgctaaa gattctcgaa ttcgtttcat taatgaaaat
     5821 gagcaccata atttaggagt tattaaatca ttttttacgc tagttaatta cgaagttgca
     5881 gatttctatt tttttagtga tcaagatgat gtatggttgc cagaaaagtt atctgtaagt
     5941 ttagaggcag caaagcataa agcatctgat gtaccattac ttgtttatac tgatttgaaa
     6001 gttgttaatc aagaattaaa tattttacaa gacagcatga ttcgggctca atcgcaccat
     6061 gccaatacga cattattacc agaactaact gagaatactg ttactggtgg gactatgatg
     6121 ataaatcatg cattagctga gaagtggttt actcctaatg atatcttaat gcatgattgg
     6181 tttttagcac tcttagcagc aagtttagga gaaatcattt atttggattt accaactcaa
     6241 ctataccgtc aacatgacaa taatgttttg ggggcgagga caatggataa gagatttaag
     6301 atacttcgtg aaggtcctaa atctatattt acaaggtatt ggaaacttat ccatgactca
     6361 caaaagcaag cttccttaat tgttgataag tatggtgata ttatgacagc taatgatctt
     6421 gagctcatta aatgttttat aaaaattgac aaacagcctt ttatgacaag attaaggtgg
     6481 ttatggaaat atggttatag caaaaatcaa tttaagcatc aagttgtgtt taaatggttg
     6541 attgcaacaa attattacaa taagagataa gatgatgaaa aaaattttac tagtgatacc
     6601 tgcttataat gaagaaggta gtattgcaaa gacagtccag actattgtag attttaaggc
     6661 gtctcgctca ctaccttttg agctggatta tattgttatt aacgatgggt caaccgatgg
     6721 tactccagag ttattagata gattgggttt aaatcatatt gaccttgttc aaaatctagg
     6781 tattggtggc tgtgtgcaga caggatactt atacgctaat cgtaatcatt acgatgttgc
     6841 cgttcaattt gatggtgatg gtcaacatga tattagaagc attgaagatg ttgttatgcc
     6901 aatcttaaat gacgaagcag attttgttat tggttctcgc tttgttgata aaaaacatca
     6961 aaattttcaa tcgacagcaa tgaggcgtct tggcataaat ttaatatctg ctgctataaa
     7021 attaacgacg ggacacaaag tctatgatac aacaagtggt tatagggcag ccaacgcggc
     7081 gctaatagct tatttatcgt gtcattatcc agtacaatac ccagagcctg aatcaactgc
     7141 tcggattttg aaaaaaggct atcgtttgaa ggaagtcaca gctaatatgt ttgaacgtga
     7201 ggcaggtact tcaagtataa gttccctaaa atcgattttt tatatgacgg atgttttaac
     7261 atcaattatt attgcagggt ttattaagga ggatgataaa tgagtttagc attgcaaatt
     7321 gaaatgttta ttttagccat tgtcctactt tatatgatta taagaatggt taataaaaac
     7381 acattttctg ttaaacgttc tttaccttgg ctattagttg gtttaattct agtctttatt
     7441 agtgtatttc ctggggtggt ctcatttatc gctcatcacc ttggattcgc tttaacgatg
     7501 aatttcttgt tatttggtgc tattttattt ctgttttgtt tagaattatt tgatactagt
     7561 agcaatacca aaagagatga gcaaattaaa cgcctgattc aagaggtttc tttgttgaaa
     7621 aaagagatag aggatagcac tttagatgag taaaaaacat atttttattt gtctgttttt
     7681 catgcttttt ttagtaggtt gccaaaataa taaaaaagta aaaacagata aaaaagtaga
     7741 tattacagct tattattatc ataataatac cccgcaatac agtgttcaga ttaatgctat
     7801 tcatttacaa acagcattgg ataaagtgag gacgggggag tttgagagca aagactttac
     7861 acactatact tttgatagta tcaagcggaa gtaccaagtt agtggtaaaa aagtccttat
     7921 ggataataac tatagatttg gttctggtat aaaaaatact agacaggatg ctattgctat
     7981 tgtaaggtta ctgcttgaaa agaataaaga ttatttaatt tacatgaatg gcttagaaga
     8041 gccaagtgtt ttatataatc cagaaaataa acgttataag ttcacaaata acaaaggaaa
     8101 agcaattggt aatattccag ttggtttaac tgcttttgaa aacagcgcag aaactcaaga
     8161 atatgtgttg aaaaatattc agaaaaacga aacgatttat cttggtaata cccgagtaga
     8221 taatcctcga gtaactgtta ataacaaaaa aagagatact ataggagttg aatacggtaa
     8281 acgtattact tataggattc caatttattc aaagcagctg actgtcagag ttagccctaa
     8341 ttttgtagta gatagtacta attataatta ccgactttct caagcgccta ttattgcgga
     8401 agaaggtaaa gagggacgtt tgtatccaat agatagcagt attagtgtcg atggtgataa
     8461 aattgtttta aatagtagtg gtgatgaaac agagcaaaaa gaattattac aaaatagcct
     8521 gtataaactt cactctattg ataagttaga ctttgatttg acaaatgctg atattaaaga
     8581 gttagagatt acaggacatg ttgcttcaaa aagagattat aatttgtctg ttgctcagga
     8641 aaagaaaggc gtcatagaga ctaaacggag tctaatttct gttgataatc aaaaacaaga
     8701 tcaagggatt tttgtcgagg attctaaagg ctatataaag acacctagtt tgagcagtta
     8761 tggtataaat tttgccatgt atgacgacaa tcaaaataga ttattgcaag gttcagagta
     8821 tattttagga agattgaatg cagataaaaa agtatctcta tatcaaccta atggcttttg
     8881 gataaatact caaaaaacga ttaagagttt aaataaagaa tatcttaacc aaaatgctta
     8941 ccatattaga ggaaataaca ttataggtat tgatggctat tcaaatgaca tagacttgaa
     9001 tcctaggtta tgggcttata actataaaaa acaagtgaaa agtaaccaat cattatttaa
     9061 attacgaggt ttgtctaata agtataagta tttcttaata cagacaaaat ctccaaaagg
     9121 ctacttacaa agcaataaac cattttattt tacagtaaat aaagatagtg tttctaaagc
     9181 acaatttggt aactataata taaatggtta tataatggac atgcagtaca acaaacaaga
     9241 atacaatgca cttgcaaaca ctccaaaagg tcaaaaaaca aagaaaactt tcaaacccat
     9301 gaccctagtg attctttttg cagtagcttt atttattttc tatattacag ctataagatt
     9361 tgtatttaaa agaatgtgag ttaactgtta gacagaggta attatgaaag aaatgacggt
     9421 ctcagaaata agagaagttc aacttgaaat gttagcttat attgataaag ttgcgcgtga
     9481 taataaaatt gaatattctt taggcggagg cagcctactg ggagcaatga gacataaagg
     9541 attcattcca tgggatgacg atattgattt gatgctagaa agaagccagt atgagcgttt
     9601 gatgaaagca ttagctgatg ctaataatag cgattttaaa ttgctacatc attctgttga
     9661 aaaaaatcta tggccatttg ccaagctgta tcacacaaag tcaatgtact taagtaaaac
     9721 agatagaatt catccttgga cagggatttt tattgatatc ttcccacttg accgattacc
     9781 agagagtgca gaagaacgtc aaagattctt taaaaaagtt cactctgctg ctgcaaattt
     9841 gatgtgtacg acttacccca attttgctag cggttcccga aagctttatg caaatgcaag
     9901 gctaattttg gggctacccc gattcatcgc ttatcacggc caagctaaaa aacgtgctga
     9961 aattgttgat caagtgatgg aaacatataa taatcaagag gttccttaca tgggttatac
    10021 cgattcaaga tatcgcttga aagaatattt tccaagagaa attttttctg agtatgaaga
    10081 tgttatgttt gaaaacatta aaacccgaaa aattaaaaat gagcatgctt atctgaatca
    10141 attatatggg ggtagttata tggaattacc tccagaaagt aagagagaaa gtcatagtta
    10201 ttacacttgg tactggaaag aaggttaatg atgaatattg gagttatttt tgcaggtggt
    10261 gttggtcgtc gcatgaatac caaaggtaaa ccaaagcaat tcctagaagt gcatggtaaa
    10321 cccattattg tacacacaat tgatattttc caaaatacag aagctattga tgctgtagta
    10381 gttgtctgtg tatcagactg gctagattat atgaataatc tggtagagcg tttcaacctt
    10441 acgaaagtaa aagcagttgt tgcaggtgga gaaactggcc aaatgtcaat ttttaagggg
    10501 cttgaagccg ctgaacagct ggctacagat gatgccgttg ttttaattca tgacggagta
    10561 cgtccattga ttaatgaaga agttattaat gctaatatac agtcagtaaa agaaacagga
    10621 agtgcggtaa cttcagttcg tgctaaagag acagttgttc tagtcaatga ttcttctaaa
    10681 atatctgaag ttgttgatcg tactcgttcc ttcattgcta aagcgccaca aagtttttat
    10741 ctatcagata tcctttctgt tgaacgtgat gccattagca aagggattac tgatgcaatt
    10801 gattctagta ctttgatggg tatgtacaat cgtgaactca ctattgttga gggaccatat
    10861 gaaaatatca agataacaac accagatgat ttttatatgt ttaaagcttt gtatgatgct
    10921 agagaaaatg aacaaattta tgggatgtaa tgatgattaa atttagtaat aaaattgtac
    10981 aagaagattt agcacttatt agtaaccaaa attatattaa ttgggaaaga attgctaata
    11041 aaggcgtttt aataagtggt tctaatagta tgttagcgag ttatatggta tttttactag
    11101 cctatctcaa tgagacaaga aattaccaaa cgcaaattat tgcgacagct agaaacattg
    11161 aaaaagcccg tgataaattt tcagatttag ttgggaaaga ttatttcacc ttaattcctt
    11221 atgatgtaga agaacgccta gagtatgatg gaaaagtgga ttacattatc catgcagcaa
    11281 gcaatgctag tccaactgct attttgagta atcctgttag catcataaaa gctaatacaa
    11341 ttggtacatt gaatttatta gactttgcaa aggaaaaaac aattgaaaac ttcttgttct
    11401 tatcaacgcg tgaagtatat ggaacatcta ttaaagaagt gattgatgaa gaagcttatg
    11461 gtggatttga tattttagct actagagctt gttacccaga aagcaaacgt atggctgaaa
    11521 ctctactgca aagttactat gatcaatata aggtaccctt tactattgct cgtattgcac
    11581 attcatttgg tcctggtatg gagcttggta acgatggtcg tattatgaat gatcttttat
    11641 cgaatgtcat tgatggcaaa gatatcgttt taaaaagtag tggtacagca gagcgtgctt
    11701 tctgttattt agcagatgct gtatcagggt tatttactat tttattaaat ggtgaagttg
    11761 gtcaagctta caatgtagca aatgaagatc aacccattat gattaaggat ttggcacaaa
    11821 agttagttga tctcttttct gataaaaata tttcagtagt attcgatatt cctaaaacta
    11881 tgagtgcagg ttatagcaaa atggggagaa cacgtctcac tatggccaaa ttagaagcgt
    11941 tgggatggaa gcgagaagtt tctcttgaat ctggaatatt aaaaacagta caagcatttg
    12001 aggagtaata tggtaaagac agcagtttta atggcgacat acaatggcga aaaatttata
    12061 tctgaacaac ttgattcaat tcgccaacag acattaaaac cagattatgt attattgagg
    12121 gatgattgtt caacggatga aacagtcaat gtcgtcaata actatatcgc aaaacatgag
    12181 ttagaaggct ggaaaattgt taaaaacgac aaaaacttag gctggcgttt aaattttcgt
    12241 caattactta ttgatgtgtt agcctatgag gttgactatg tcttttttag tgatcaagat
    12301 gatatttggt atcttgataa aaacgaacga cagtttgcca ttatgtcaga taaccctcaa
    12361 attgaggttt tgagtgcaga cgttgatatc aaaacgatgt ctacagaagc cagtgttcca
    12421 cattttctaa ctttttcttc tagtgataga atcagtcagt atcctaaagt atatgattat
    12481 caaacattcc gtcccggatg gaccattgct atgaagagag attttgcgca agctatcgct
    12541 gataattgga cttttggagc taatatttcg cacgataatc ttatcgagac aactgccagt
    12601 attattggaa atggttataa ccttaacgaa agcgtaggag ttcatattcg atatggagga
    12661 aacgcaagtg gtaatagcaa atttgttttg aattggaagt cgcctaaaac acaacatatc
    12721 acaggattaa aaacatttgt tgatttttac gaaaccatgc agaaggttgt caacaaatat
    12781 caaaatgatg tcgctttaaa tcaacgctta gcctttgaaa aagaaagatt ggaagcttct
    12841 aaattgtcat taattcataa tagtgctttt gtgattaaaa attggcgact ctataactct
    12901 aatagtggac gtatgagaga tttattattt gcgtttaaaa agtaaggaat aatatggtaa
    12961 aaacagcagt tttgatggca acatataatg gtcaaggttt catccacgat cagctagatt
    13021 caattagaaa tcaaacgttg agaccagact atgtcttaat gagggacgat ggctctactg
    13081 atgatactgt aaaggtggtt gaagactata taaaagagca ccgtttagat ggttggtcaa
    13141 taactagtaa tgataaaaat ttaggttggc gtttaaattt tcgtcaatta cttattgatg
    13201 tgttagccta tgaggttgac tatgtctttt ttagtgatca agatgacaca tggtatcatc
    13261 ataaaaataa gatgcaagtt gatattatgg aagaacgcca agatataaac cttcttagcg
    13321 ccgatattga tatcaaaaag ttatcagaag aggctactat tcccaataat tttaagttca
    13381 atggtgagga aacaatttct aagtatccat tagacttttc atatcataac tatcgtcaag
    13441 gttggacttt ttgtttacgc aagtcgtttg ttgatcaagt aatgactttt tacagtgata
    13501 atttaatttt gtcacatgat aatctttttg caggggtatc tggtgtcatt ggttcaggtt
    13561 acaatttaaa cagaagtgtt ggtgaacata agcgacatgc tgagaatgca agtgggaata
    13621 tcttgaatat gagctcaagt tacgaaaggc atttacaaga actgtatttt gttttgagtt
    13681 actatatcat ccttgtacca tttttagaaa aaagtagctc tagtcaatta agggaagcta
    13741 aagcttatct ggattttaat cagcaacgtt ataaaaatgc tcaaaataaa gattttgtag
    13801 caatacttaa acaaatgatg actaaaaaac ggttctatga tagcttttca aatcgcattc
    13861 gtgacttggt gtttttatta aaagatataa aaaggagtta atatgaacct caaaaaagtt
    13921 cgtctatttg aaaagttttt tctagacaag aacttgcata aaacatattt tatattaggc
    13981 ttaacgatta ctattgtatt gagcctatta atgccatttt tcaatgaacc tgatggacaa
    14041 taccatttag ctgtgtctgg tcgtatttca aataacatta ttgatacttc ttactatgga
    14101 gaatataata ttgattcagg tatgagacga caatcgacct cctatagaga tggtactaga
    14161 tttgaaaaat actatttaaa taaagccaat tttgttgatg gtaatcattt gcctcgtgag
    14221 ataaactatt ctaaacataa ctttgttttt tggggtcatc ttgtgccagc agttggctta
    14281 tttttaggca aatttgtgta tccttctatg ggggtaatga ttacagtagc aaggttattt
    14341 agttcctttg tctcaatcac cttggttgcc ctcattatcc ggtatgtgaa aaaaggaaag
    14401 ttaatctact ttgctatttt cctcagtccc gtagttctaa attcatttgc tagtctatct
    14461 tatgactcga ctggatatat tgtatttgga gcatttttat cgcttttgat aaatctttta
    14521 gttgataaat cgttgactaa aaatcgtagt attgctctta taggattgat tcttttagtc
    14581 attgttgcat gtaagcagaa ttactggctg tttttattgc tagtaatacc ggtattattg
    14641 caggttaaca atcctaaatt aatgggctat aatcagaagc tatcacattt atttcaaggt
    14701 cttaaatcta aaaagctatt ttggctatgt ctaacctttt tgtcacttgc tatagtttat
    14761 gttctgaccg caagacatgg tggtgtcctt gctgttttaa gacgttatgt aatgacattt
    14821 ggctataatt atgctggtat caatatttta agtaatgaca ttacttcctg gcttgcctct
    14881 ccatatccaa gtcagaattt tattcccact tgggtatcat cagtatggta tctcatggtc
    14941 tttctagtat ggttttctga agaaaaattt gttaagtcaa aaaatatggg atttcttttc
    15001 ttagctagtt tctttgtggg aattcttggt gtctttttta tcatgttaga atatcgtgga
    15061 gcgccaacaa gctacattga aggagttcaa ggacgttact ttactgttac attgcttttg
    15121 gtacaagttt ttgcatcaag tattccagcg cgtctaaatg aggcaggaag gaaagtggtg
    15181 ccgtatgcgt taactggctt ggtaattgta tcaaatgcct tattgttatt tgatactgtt
    15241 attagtttaa ttatgaggag aaactaatga aacttttgaa aaatatgttt tataatacct
    15301 cataccagtt attgactttg ttgttacctt tagtaacagt tccttatgtt tcccgtgtac
    15361 tatctcctca aggcatcggt attaatgcct atacgagctc cattgtcatg tactttactt
    15421 tatttggtgc tttagggata tctttgtacg gtaatcgtga gatagctttt gtgcagtcaa
    15481 ataagtataa gagaagtaag atattttggg aattagttgt attaaaactt gcctctgtat
    15541 ctatagcaac gttattattc tttggatttg tcttacttac aaacgaatgg caattattct
    15601 atttaattca aggtattaat ttattagcga ctgcaactga tatttcgtgg tattttattg
    15661 gagttgaaga ctttaagata attgtcattc gtaataccat tgttaagctt attacagttg
    15721 ttttgacctt cttagttgtg aagacgccag atgacctagc actttatatg tttttaattg
    15781 catttgcatc attactcgga aatttaacag tctggcatca tttaaagcat gaaatcatca
    15841 agataccatt tagtagatta gatatcttaa tacatctaag accaacttta atgttatttt
    15901 taccccaaat caccatgcag atttatctct ctcttaacaa gagtatgctg ggagcaatgg
    15961 atagtgtggt cagtgctgga tattttgatc aatctgataa aattattcgt attttattta
    16021 ccattgtttc agcaatcgga ggagttttct taccaaggct ctccagtcta ttttcttcag
    16081 gaaaagaaaa gcaagctaag gctttacttt tgaaacttgt tgatttaagc aatgctatct
    16141 caatgttaat gattgcggga gtggtaggag tttcgtcgac atttgccgtc ttcttttttg
    16201 ggaaagggta tgaagctgtc ggtccgctaa tggctgttga gtctttgatg atcatttgca
    16261 tttcttatgg taacgcttta gggacacagt atctactggc ttcaagacgt acaaaagctt
    16321 acacaatgtc tgcagttatt ggacttgtgg caaatgttgt gcttaatata cttttaatac
    16381 caattttagg tgctatggga gctattatct ctacagttat tactgaattt attgtctccc
    16441 tctatcaagc tatctcttta agagatgtat ttacttttaa agaattaaca agaggaatgc
    16501 tgcgctattt aattgctgct actcttagtg gagctgtact ttattatatc aatactcaaa
    16561 tgtcagtatc attggtaaat tatgtcattc aaagtttagt agcagtaact atctatgtcg
    16621 gtatcgtttt tataaccaaa gcaccagtca ttcagttatt tagagatttt agaaaagagc
    16681 atagaacatg aaatatttag cgggtattgt cacctttaac cccaatattg aacgcttaga
    16741 tcagaacatt cgagcaattt atcctcaagt aagccatatc tacatcgtag ataatggctc
    16801 gaaaaataag gaagaaattt ctcaattagt ggccgattat aatgaagagg ggcacctaac
    16861 tgttgattat ttaacagaaa ataaaggaat tgcatatgct ttgaactgta tcggtcaatt
    16921 tgcagtagca caagaatttg attggttctt gactttagat caagattcgg ttgtcctagg
    16981 tgatttgata gataattatg aaaattacct tcttttacct aaggtcggta tgttatcttg
    17041 tttgtatcaa gatatgaacc gtgaagattt agttatgcaa gaatttgact ataaagaaat
    17101 tgaagagtgt attacttcgg ctgctttaat gaaaacgagc gtttttgaag aaacatctgg
    17161 ctttgcagaa gagatgttta ttgattttgt tgactctgaa atgaattatc gtctgtcaga
    17221 gatgggatat aagacttatc aggttaattt tattggtctt ttacacgaaa ttggacactc
    17281 ttcacgagtt aaaaaatttg gccatgtttt tcatgtattg aatcattcac catttcgaaa
    17341 atattacatg atacgcaatg cgatttatat tataaaaaaa tatggtaaga agaagcggca
    17401 caaatattta gtatttatgc gaaatgaatt tgttcgagtg ttggtagcag aagagcaaaa
    17461 gagcaaaaaa ttagttgcta tgattaaagg cttaaaagat ggcttattga tgaaagtgga
    17521 taaatatgga aaataaggta aaaacagttg ctgttttctc aggatattat ctacccttcc
    17581 tcggaggtat tgaacgttat acagataaaa tgacagcaga tttggttaag cgtggctacc
    17641 gtgttgttat tgtaacaact aatcatgatg atttaccgat tatagatgaa gacaagggta
    17701 gaaaaattta ccgtttaccg accaaaaata ttgtcaagca gcgctatcct attattaaca
    17761 aaaatcgaga atacaacacg ctgatgaaac atgtttcaga tgaaaatatt gattttgtga
    17821 tttgtaatac acgttttcag ttaacaacgc tagaagggct atcttttgca aagaagcacc
    17881 gtttacctag tattgtttta gaccatgggt caagtcattt ttctgtcaat aatcgttttt
    17941 tagatttctt tggagctatc tatgaacatc ttctcacagc gcgtgttaag cactatcgtc
    18001 cagattttta tgcggtatct aagagaagcg ttgagtggtt aaaacatttt aatatagaag
    18061 ctaaaggagt tatttataat tcggtatctg aaagtctagg ttctgatttt gctgacacgg
    18121 cttaccttga aaaatctgct gatgatattt tcatcactta tgctggtaga attattaaag
    18181 aaaaaggcat tgaattgctc ttagaagcat tttcgatgtc acagtactct gaaaatgttt
    18241 atttacagat tgcaggagac ggacctgaat tagcacattt gaaagagaaa tatcagtcca
    18301 aacagattaa ctttttgggg aaattaaact ttgaacagac tatgtcttta atggcacaga
    18361 cagatatctt tgtttaccca tcaatgtatc cagagggatt accaacctct attttagaag
    18421 ctggtctact ttcgagtgct attattgcaa cagatcgcgg tggaacagtt gaagttattg
    18481 atagccctga gttaggaatc atcatggaag aaaatactca gtcactccat gaatctcttg
    18541 atttattagt aaaagataag gcattaagag agaaactaca acagaatatt gctaaaagaa
    18601 ttaaagaaca tttcacatgg gaaaaaacag ttgaaaagtt ggattatatt attcaaaaag
    18661 aacttaatct gtaatagatt atgtcactaa attgacgcta ttgtcctatt g
//
LOCUS       DBJYSH010000035        18317 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.035, whole genome shotgun sequence.
ACCESSION   DBJYSH010000035 DBJYSH010000000
VERSION     DBJYSH010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 18317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18317
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.035"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            complement(80..874)
                     /locus_tag="ACUBV3_000918"
     CDS             complement(80..874)
                     /locus_tag="ACUBV3_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T family protein"
                     /protein_id="HHU3847431.1"
                     /translation="MDKLILGRYIPGNSLIHKLDPRSKLLAMLLFIIIVFWANNVVTN
                     VIVFIFTLVIVGLSQIKFSYFFNGIKPMVGIILFTTLFQMLFAQGGQVIFSFWIFSIT
                     SLGLQQAALIFMRFVLIIFFSTLLTLTTTPLSLADAVESLLKPLEVLRVPAHEIGLML
                     SLSLRFVPTLMDDTTRIMNAQRARGVDFGEGNLIHKVKSIIPILIPLFASSFKRADAL
                     AIAMEARGYQGGANRSKYRLLKWTVRDTFSILLMLLLGLSLFLLKN"
     gene            complement(867..1709)
                     /locus_tag="ACUBV3_000919"
     CDS             complement(867..1709)
                     /locus_tag="ACUBV3_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000510607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HHU3847432.1"
                     /translation="MGIEFKNVSYTYQAGTPFEGRALFDVNLKIEDASYTAFIGHTGS
                     GKSTIMQLLNGLHIPTKGEVIVDDFSIKAGDKNKEIKFIRQKVGLVFQFPESQLFEET
                     VLKDVAFGPQNFGISQIEAERLAEEKLRLVGISEDLFDKNPFELSGGQMRRVAIAGIL
                     AMEPKVLVLDEPTAGLDPKGRKELMTLFKNLHKKGMTIVLVTHLMDDVADYADYVYVL
                     EAGKVTLSGQPKQIFQEVELLESKQLGVPKITKFAQRLSHKGLNLPSLPITINEFVEA
                     IKHG"
     gene            complement(1685..2524)
                     /locus_tag="ACUBV3_000920"
     CDS             complement(1685..2524)
                     /locus_tag="ACUBV3_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HHU3847433.1"
                     /translation="MTNIITVNNLFFKYDSNQTHYQLENVSFHVKQGEWLSIIGHNGS
                     GKSTTVRLIDGLLEAESGQIIIDGQELTEDNVWELRHKIGMVFQNPDNQFVGATVEDD
                     VAFGLENKGIPLKDMKERVDQALDLVGMSEFKMREPARLSGGQKQRVAIAGAVAMRPQ
                     VIILDEATSMLDPEGRLELIRTIRAIRQKYNLTVISITHDLDEVALSDRVIVMKNGKV
                     ESTSTPKALFGRGNRLISLGLDVPFTSRLMAELAANGLDIGTEYLTEKELEEQLWELN
                     LKM"
     gene            complement(2524..3066)
                     /gene="pgsA"
                     /locus_tag="ACUBV3_000921"
     CDS             complement(2524..3066)
                     /gene="pgsA"
                     /locus_tag="ACUBV3_000921"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003090075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HHU3847434.1"
                     /translation="MMKKENIPNLLTVVRILMIPLFIVLTSVTTSTTWHIVAAIVFAI
                     ASLTDYLDGYLARKWQVVTNFGKFADPLADKMLVMSAFIMLVGLDLAPAWVSAIIICR
                     ELAVTGLRLLLVETGGTVLAAAMPGKIKTATQMFAVIFLLVHWMTLGNIMLYIALFFT
                     LYSGYDYFKGAGFLFKDTFK"
     gene            complement(3189..4472)
                     /gene="yfmH"
                     /locus_tag="ACUBV3_000922"
     CDS             complement(3189..4472)
                     /gene="yfmH"
                     /locus_tag="ACUBV3_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000217763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P 5-aminopentanol modification-associated
                     protein YfmH"
                     /protein_id="HHU3847435.1"
                     /translation="MTVNKITYQNLQEEVYKLTLESGLNVYLIPKPSFKETVGVLTAN
                     FGSLHTKYTRNGCVEHYPAGIAHFLEHKLFELDKGQDAATQFTKYGAESNAFTTFDKT
                     SFYFSTISHITNCLDILLDFVLTTNFTEESITKEKDIIKQEIEMYQDDPEYRLYQGVL
                     SNLYPNSPLAFDIAGDYQSISQITLTDLQENHKDFYQLSNMNLVLVGQFSPQEIITYL
                     QKNSHFTSYSQNIDRDSISLEPVIKNNSCHMTVTKPKLAIGYRKSNHMIHGSYLKEKI
                     GLQLFFAMLLGWTSTINQDWYESGQIDDSFDIEIEVHPDFECVIISLDTTEPIAFSTQ
                     LRLLLKNALQSSDLTESHLKNVKRELYGDFLRSLDSIENLAMQFVTYLYDGKTMYLDL
                     PSIVEELDLEDVITIGKDFLYNADTSDFVIFPKSS"
     gene            complement(4474..5718)
                     /gene="yfmF"
                     /locus_tag="ACUBV3_000923"
     CDS             complement(4474..5718)
                     /gene="yfmF"
                     /locus_tag="ACUBV3_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P 5-aminopentanol modification-associated
                     protein YfmF"
                     /protein_id="HHU3847436.1"
                     /translation="MKIVEGVSLHLIKNQQFKTNHLTFRFSGDFNNKTVARRSLVAQM
                     LVTANAKYPKVQEFREKLASLYGASLSTKISTKGLVHIVDIDIVFVKNTFTLEQENIV
                     EQIITFLEDMLFSPLISLEQYQTSIFDTEKKNLIQYLEADIEDNFYSSDLALKSLFYN
                     NKTLRLPKYGTASLVESENSFTAYQEFQKMLKEDQLDIFVVGDFDDYRMIQAFNRMAF
                     EPRHKVLAFDYTQTYENITRSQVEDKDVNQSIMQLAYHLPITYKDEDYFALIVFNGLF
                     GAFAHSLLFTEIREKQGLAYTIGSQFDSFTGLFTIYAGIDKENRERFLKLINKQFNNI
                     KMGRFSSTLLKQTKDILKMNYVLASDNPKVIVDHIYHEHYLDQFHTSALFIDKVDDVT
                     KSDIVSVATKLKLQAFYFLEGN"
     gene            5957..6310
                     /gene="yaaA"
                     /locus_tag="ACUBV3_000924"
     CDS             5957..6310
                     /gene="yaaA"
                     /locus_tag="ACUBV3_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000384871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HHU3847437.1"
                     /translation="MDYKLFDEYITLQSLLKEIGIIQSGGAIKKFLADNRVLFNGDLE
                     NRRGKKLRLGDIITIPDQNIEIIIRKPSDQEIEERNIEIAEKQRVSAIVKEMNKNTNK
                     GKSKTSKKPVRFPGT"
     gene            6313..7422
                     /gene="recF"
                     /locus_tag="ACUBV3_000925"
     CDS             6313..7422
                     /gene="recF"
                     /locus_tag="ACUBV3_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632366.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HHU3847438.1"
                     /translation="MWIKNISLKHYRNYEEAQVDFSPNLNIFIGRNAQGKTNFLEAIY
                     FLALTRSHRTRSDKELVHFKHHDVQITGEVIRKSGHLNLDIQLSEKGRITKVNHLKQA
                     KLSDYIGAMTVVLFAPEDLQLVKGAPSLRRKFLDIDIGQIKPTYLAELSNYNHVLKQR
                     NTYLKTTNNVDKTFLTVLDEQLADYGSRVIEHRFDFIQALNDEADKHHYIISTELEHL
                     SIHYKSSIEFTDKSSIREHFLNQLSKSHSRDIFKKNTSIGPHRDDITFFINDINATFA
                     SQGQQRSLILSLKLAEIELIKTVTNDYPILLLDDVMSELDNHRQLKLLEGIKENVQTF
                     ITTTSLEHLSALPDQLKIFNVSDGTISINEKKATD"
     gene            complement(7434..8300)
                     /locus_tag="ACUBV3_000926"
     CDS             complement(7434..8300)
                     /locus_tag="ACUBV3_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HHU3847439.1"
                     /translation="MEGLLIALIPMFAWGSIGFVSNKIGGRPNQQTFGMTLGALLFAI
                     IVWLFKQPEMTASLWIFGILGGILWSVGQNGQFQAMKYMGVSVANPLSSGAQLVGGSL
                     VGALVFHEWTKPIQFILGLTALTLLVIGFYFSSKRDVSEQALATHQEFSKGFATIAYS
                     TVGYISYAVLFNNIMKFDAMAVILPMAVGMCLGAICFMKFRVNFEAVVVKNMITGLMW
                     GVGNVFMLLAAAKAGLAIAFSFSQLGVIISIIGGILFLGETKTKKEQKWVVMGILCFV
                     MGAILLGIVKSY"
     gene            complement(8356..9018)
                     /locus_tag="ACUBV3_000927"
     CDS             complement(8356..9018)
                     /locus_tag="ACUBV3_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001868421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HHU3847440.1"
                     /translation="MKNYGKILKELREDKGISLSSLAKSAQLSKSTLSRFENGETQIG
                     IDKFIKALQTLEVGVTINEVSILDSKVKAGTSNTDLEQLTLLESYRDNEDIMRIFSFQ
                     KQQSCDRIESNVLKILAKLFISNLGLNMRLPQDEINLVVTYLNGVTQYNDFYFKVICY
                     FQDILPEDVILNKISNMTKEQLPYSKSLVNLLIKQVIIALEKDSVDKAIVFADLANKV
                     SQ"
     gene            complement(9093..10574)
                     /gene="guaB"
                     /locus_tag="ACUBV3_000928"
     CDS             complement(9093..10574)
                     /gene="guaB"
                     /locus_tag="ACUBV3_000928"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003085743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HHU3847441.1"
                     /translation="MSNWDTKFLKKGFTFDDVLLIPAESHVLPNEVDMKTKLADNLTL
                     NIPIITAAMDTVTDSKMAIAIARAGGLGIIHKNMSIVDQAEEVRKVKRSENGVIIDPF
                     FLTPDNTVSEAEELMQNYRISGVPIVETLENRKLVGIITNRDMRFISDYKQLISEHMT
                     SQNLVTAPIGTDLETAERILHEHRIEKLPLVDDERRLSGLITIKDIEKVIEFPKAAKD
                     EFGRLLVAGAVGVTSDTFERAEALFEAGADAIVIDTAHGHSAGVLRKIAEIRAHFPNR
                     TLIAGNIATAEGARALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQITAIYDAAAVAR
                     EYGKTIIADGGIKYSGDIVKALAAGGNAVMLGSMFAGTDEAPGETEIFQGRKFKTYRG
                     MGSIAAMKKGSSDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIVFQMLGGIRSGMGY
                     VGAANIKELHDNAQFVEMSGAGLKESHPHDVQITNEAPNYSVH"
     gene            complement(10731..11216)
                     /locus_tag="ACUBV3_000929"
     CDS             complement(10731..11216)
                     /locus_tag="ACUBV3_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HHU3847442.1"
                     /translation="MNKKETRHQLIRSLVSETKVRTQHELRELLEKNGVSVTQATLSR
                     DMKELNLIKVNESSDNATETYYEIHSISQKRWEERLRFYMEDALVMLFPVQNQIVLKT
                     LPGLAQSFGSILDSILLPEILATVCGDDTCLIICNNSEEAQKCFEKLSQYTPPFFFSK
                     K"
     gene            complement(11226..11906)
                     /locus_tag="ACUBV3_000930"
     CDS             complement(11226..11906)
                     /locus_tag="ACUBV3_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HHU3847443.1"
                     /translation="MITKEQYFYFRQLDDFKHFTIEQFDHIVSHIKHRTALKNHTLFF
                     EGDYREKLFLIQSGHVKIEQSDASGSFIYTDYVRQGTVFPYGGLFLDDDYHFSAVAIT
                     DIEYFSLPMALYEEYSLQNINQMKHLCRKYSKLLRVHEIRLRNMVTSSASMRVIQSLA
                     TLLLQVPTERGHLPFPITTIEIANMSGTTRETVSHVLKELRQKDIVEMKGKKLLYNNK
                     NYFKKFIE"
     gene            12114..12818
                     /locus_tag="ACUBV3_000931"
     CDS             12114..12818
                     /locus_tag="ACUBV3_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="B3/B4 domain-containing protein"
                     /protein_id="HHU3847444.1"
                     /translation="MTKFIVDSSYWNLFPTSKIGVILIKDYHMDRNLETELKQLLSDS
                     HSLAKKYLQEKEFSQNRVIQTYRKAYQTFKTKKGARSSIEALLKRVNSGNEITSINPL
                     VDIYNAASLRFGLPIGAEDSDTFRGDLKLTITNGGDEFYLIGEDFNRPTLSGELAYVD
                     DVGAVCRCFNWRDGKRTMITDNTQNAFLVIELIDNGREIIFKEALDFIATNTNRFLKA
                     KTQTIILDKEHSEITL"
     gene            13085..14317
                     /gene="arcA"
                     /locus_tag="ACUBV3_000932"
     CDS             13085..14317
                     /gene="arcA"
                     /locus_tag="ACUBV3_000932"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HHU3847445.1"
                     /translation="MTQTHPIHVFSEIGKLKKVMLHRPGKEIENLMPDYLERLLFDDI
                     PFLEDAQKEHDAFAQALRNEGVEVLYLENLAAESLTNQEIREQFIDEYIGEANVRGRA
                     TKKAIRELLLNIKDNKELIEKTMAGIQKSELPEIPSSEKGLTDLVESNYPFAIDPMPN
                     LYFTRDPFATIGNGVSLNHMFSETRNRETLYGKYIFTHHPEYGGKVPMVYEREETTRI
                     EGGDELVLSKDVLAVGISQRTDAASIEKLLVNIFKQNLGFKKVLAFEFANNRKFMHLD
                     TVFTMVDYDKFTIHPEIEGDLRVYSVTYENQDLHIEEEKGDLADLLAKNLGVEKVELI
                     RCGGDNLVAAGREQWNDGSNTLTIAPGVVIVYNRNTITNAILESKGLKLIKINGSELV
                     RGRGGPRCMSMPFEREDL"
     gene            14323..14754
                     /locus_tag="ACUBV3_000933"
     CDS             14323..14754
                     /locus_tag="ACUBV3_000933"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HHU3847446.1"
                     /translation="MKLLVNHLPYQRAASLYIRFSVFVIERNIKMEEEFDDNDEQDTI
                     YAVLYDGKQPVSTGRFLPETQTEARLTRIATLKGYRGNGYGTKIIIALENYAKENGYH
                     YLTIHAELTAKDFYQTLGYQATGNIYMEDGEACQTLEKYLI"
     gene            14770..15783
                     /gene="argF"
                     /locus_tag="ACUBV3_000934"
     CDS             14770..15783
                     /gene="argF"
                     /locus_tag="ACUBV3_000934"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000195397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - L-arginine biosynthetic process
                     via ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HHU3847447.1"
                     /translation="MTQVFQGRSFLAEKDFSREEFEYLIDFSAHLKDLKKRGVPHHYL
                     EGKNIALLFEKTSTRTRAAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRMF
                     DGIEFRGFSQRMVEELAEFSGVPVWNGLTDEWHPTQMLADYLTIKENFGKLEGITLVY
                     CGDGRNNVANSLLVAGTLMGVNVHIFSPKELFPAEEIVKLAEEYAKESGAHVLVTDNV
                     DEAVKGADVFYTDVWVSMGEEDKFKERVELLQPYQVNMELIKKANNDNLIFLHCLPAF
                     HDTNTVYGKDVAEKFGVKEMEVTDEVFRSKYARHFDQAENRMHTIKAVMAATLGNLFI
                     PKV"
     gene            15846..17273
                     /gene="arcD"
                     /locus_tag="ACUBV3_000935"
     CDS             15846..17273
                     /gene="arcD"
                     /locus_tag="ACUBV3_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043858 - arginine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HHU3847448.1"
                     /translation="MEKEKKLGLLPLTMLVIGSLIGGGIFDLMQNMSSRAGLVPMLIA
                     WVITAIGMGTFVLSFQNLSEKRPDLTAGIFSYAKEGFGNFMGFNSAWGYWLSAWLGNV
                     AYAALLFSSLGYFFKFFGNGNNIISIIGASIVIWVVHFLILRGVNTAAFINTIVTFAK
                     LVPVIIFLISALLAFKFNIFSLDIWGNGLHQSIFNQVNSTMKTAVWVFIGIEGAVVFS
                     GRAKKHSDIGKASILALFTMISLYVLISVLSLGIMSRPELANLKTPAMAYVLEKAVGH
                     WGAILVNLGVIISVFGAILAWTLFAAELPYQAAKEGAFPKFFAKENKNKAPINSLLVT
                     NLCVQAFLITFLFTQSAYRFGFALASSAILIPYAFTALYQLQFTLREDKSTPGHQKNL
                     IIGILATIYAVYLIYAGGFDYLLLTMIAYTLGMILYIKMRKDDKLPIFVGYEKISAIV
                     ILALCLLCIIEIMTGQIDIAAILAG"
     gene            17294..18250
                     /gene="arcC"
                     /locus_tag="ACUBV3_000936"
     CDS             17294..18250
                     /gene="arcC"
                     /locus_tag="ACUBV3_000936"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HHU3847449.1"
                     /translation="MVKEHQKIVVALGGNAILSTDASAKAQQEALINTSKSLVKLIKE
                     GHDVIVTHGNGPQVGNLLLQQAASDSEKNPAMPLDTCVAMTEGSIGFWLQNALNNELQ
                     EQGIDKEVATVVTQVIVDEKDQAFTNPTKPIGPFLSEEDAKKQAQETGSKFKEDAGRG
                     WRKVVPSPKPVGIKEASVIRRLVDSGVVVISAGGGGVPVIEDANTKALKGVEAVIDKD
                     FASQTLSELVDADLFIVLTGVDNVFVNFNKPNQEKLEEVTVSQMKQYITENQFAPGSM
                     LPKVEAAIAFVENKPESRAIITSLENIDNVLAQNAGTQIVAN"
BASE COUNT         6150 a         3342 c         2967 g         5858 t
ORIGIN      
        1 agttttcttt aagtaaagtt gttgagcttt tcacattata taaatgaaaa ggctaagacc
       61 ataaactgat cttaaccttt taatttttta atagaaacaa actcaaaccc aataataaca
      121 taagtaatat actaaaagta tcccgaacag tccacttcaa taaacggtac ttactacgat
      181 tggctccgcc ttgataacct cttgcttcca tagcaattgc caacgcatct gctcttttaa
      241 aactagaagc aaataatgga atcaaaatag ggataataga cttaacctta tgaattaggt
      301 ttccctctcc aaaatcaact ccacgagctc tttgagcatt catgatacgt gttgtatcat
      361 ccattaaagt aggaacgaaa cgtaagctaa gggataacat caaaccaatc tcgtgtgctg
      421 gtactctcag tacttccaat ggttttaaca aagattcaac agcatctgct aaacttaacg
      481 gagtagtagt taaggtaagt aatgtcgaaa agaagataat taaaacaaat ctcataaaaa
      541 taagtgctgc ttgttgtaac ccaaggctag tgatgctaaa aatccaaaac gagaagataa
      601 cctgcccgcc ttgtgcaaac agcatctgga agagggttgt aaataaaata ataccaacca
      661 tcggtttaat accattgaaa aaataggaaa attttatctg agataaaccc acaataacca
      721 acgtaaaaat gaatacgatc acatttgtca ctacattatt tgcccaaaaa acaataatga
      781 taaaaagcaa catggctagt aacttacttc gaggatctaa tttatgaatg agagaattac
      841 ctggtatgta acgtcccaaa atcaatttat ccatgcttaa tagcctccac aaattcgtta
      901 atagtaattg gtaaactagg taaatttaat cccttatgag atagtctttg agcaaacttg
      961 gtgattttgg gaactcctaa ttgtttactt tctaaaagtt ctacttcttg aaaaatctgt
     1021 tttggttgtc ctgataaggt tactttccct gcttctaaaa catacacata gtcagcataa
     1081 tccgctacat cgtccattaa gtgagtcact aagacgatag tcattccttt tttatgaaga
     1141 tttttaaaaa gagtcattaa ttcttttctt cccttaggat caagtccagc tgttggctca
     1201 tccagtacta gtactttggg ttccatcgct aaaataccag ctatagcaac ccgcctcatc
     1261 tgccctccag aaagttcaaa tggattttta tcgaataaat cctcactgat accaactaac
     1321 cttaattttt cttcagccag cctttcagct tcaatctgag aaataccaaa attttgtggt
     1381 ccaaaagcaa catcctttaa aactgtctct tcaaaaagct gactttctgg aaattgaaaa
     1441 actaaaccaa ctttttgcct tataaatttg atttctttgt tcttgtcccc tgctttaata
     1501 gaaaaatcat cgacaattac ctcacctttt gtaggaatat gtaaaccatt caaaagttgc
     1561 ataatagttg attttccaga acctgtgtgc ccaatgaacg cggtatagga agcatcttca
     1621 attttcagat tgacgtcaaa aagggcacgc ccttcaaaag gagtgccggc ttgataggta
     1681 taacttacat ttttaaattc aattcccata attgttcttc taattccttc tctgtaagat
     1741 actctgtccc tatatcaaga ccattagcag ccagttctgc cattaaccta cttgtaaagg
     1801 gaacatctaa acctaaacta atcaaacgat ttccacgacc aaataacgct tttggagtgg
     1861 atgtcgactc aactttacca tttttcataa cgatgacacg atcactcaat gcaacttcat
     1921 ctaagtcgtg tgtaattgaa ataacagtaa gattatattt ttgacgaata gctcgtatcg
     1981 ttcttatcag ttccaatcgt ccctcaggat caagcatact agtcgcctca tccaagatga
     2041 taacttgtgg acgcatagct acagctcctg caatggctac acgctgcttt tgtcctccag
     2101 ataaacgtgc aggctccctc attttaaatt cagacattcc taccaaatca agtgcttggt
     2161 ccactctttc tttcatgtct ttcaaaggaa tacctttatt ttccaaacca aaggcgacgt
     2221 catcttcaac agttgcccct acaaattggt tatcaggatt ttgaaatacc atgccaatct
     2281 tgtgacgtaa ctcccagaca ttatcttcgg ttaactcttg tccatcaatg ataatctggc
     2341 cagactccgc ctctaaaagt ccatctatta aacgaacagt agtggatttc ccagaaccat
     2401 tatgaccaat aatagatagc cattctcctt gtttcacatg aaacgataca ttctccaatt
     2461 gataatgtgt ttgattacta tcatatttaa aaaagagatt attaacggta ataatatttg
     2521 tcattattta aaagtatcct taaataagaa gcctgcacct ttaaaatagt cgtagcctga
     2581 atacaaggta aagaatagag caatgtataa catgatgtta ccaagtgtca tccaatgtac
     2641 taacaaaaaa ataactgcaa acatttgagt agccgtcttt attttccctg gcatagctgc
     2701 agcaagtact gttccacctg tttcaactaa aagtaaacgc aaacctgtaa ctgctaactc
     2761 tctacaaatg ataatagctg aaacccaagc tggtgctaag tctagtccaa ctaacatgat
     2821 aaaagcactc ataactagca ttttatcagc taatgggtct gcaaatttcc caaaatttgt
     2881 tactacttgc catttacgtg ctagataacc atctaggtaa tcggtcaaac ttgcaatagc
     2941 aaatacaata gctgcaacaa tatgccaagt tgtacttgtt gttactgaag ttaatactat
     3001 aaataacgga atcattaaaa ttctgactac agttaataga ttcggaatat tttctttctt
     3061 catcataaat tctccttttg gaaacatagt attcctatat tatgttattt caaaactagt
     3121 aaaagacttt tgacatacca actttataga tgtctaagct tccttattat atcaaaattt
     3181 cagattattt acgaagattt tggaaaaata acgaagtcag atgtgtcagc attatataag
     3241 aaatccttac caatcgtaat gacatcttcc aaatctaatt cttcaacgat agatggcaaa
     3301 tccaaataca tcgtttttcc atcatataaa taggtgacga attgcattgc gagattttca
     3361 atagaatcta agcttcttaa aaaatcgcca tagagctctc tcttaacgtt ttttaagtgg
     3421 ctttcggtaa gatcagatga ttgcaaagca ttttttagca ataacctcaa ctgagttgaa
     3481 aaagcaattg gctctgtcgt atctaaagaa ataataacgc actcaaaatc tggatgaact
     3541 tcaatttcta tatcaaaaga atcatcaatt tgaccagatt cgtaccagtc ttggttaatt
     3601 gtagaagtcc atcctaaaag cattgcaaaa aatagctgta atccgatttt ctcttttagg
     3661 taagaaccat gtatcatatg atttgatttt cgataaccaa tagctaattt aggttttgtc
     3721 actgtcatat ggcaagagtt gttttttata acaggctcaa ggctaataga atctcggtca
     3781 atattctgtg agtaacttgt gaaatgagaa tttttttgaa ggtaagttat aatttcttgt
     3841 ggactaaatt gtccaacaag aactaaattc atattagaca actgataaaa gtctttgtga
     3901 ttttcttgta aatcggttaa ggttatttga gagattgatt ggtaatctcc agcgatatca
     3961 aatgctaatg gagagttggg gtaaaggttc gataacactc cttgataaag cctatattct
     4021 gggtcatctt gatacatttc aatttcttgt ttgataatat ctttttcttt agtaattgac
     4081 tcttctgtaa agtttgttgt caatacaaag tcaagtagta tatctaagca gtttgtaatg
     4141 tgacttatag ttgaaaaata aaaactagtt ttatcaaaag ttgtaaaggc gttactctcg
     4201 gcgccatatt tagtaaattg agttgcagcg tcttgtcctt tatccagttc aaataattta
     4261 tgttctaaga agtgagcaat tcctgccgga taatgttcta cacaaccatt cctcgtatat
     4321 tttgtatgga gtgatccaaa atttgctgtc aatactccaa cagtttcctt aaatgatggc
     4381 tttggaataa ggtatacatt tagtccgctc tctaatgtta acttatatac ttcctcctga
     4441 aggttctgat aagttatttt attaactgtc atcctagttt ccttccaaaa aataaaaagc
     4501 ttgtaatttt aattttgttg ctacgctgac aatatctgat tttgttacat cgtctacttt
     4561 gtcaatgaat agtgctgaag tatgaaactg atctaaataa tgttcatgat aaatgtggtc
     4621 aactatgacc ttaggattat cagaagctaa aacatagttc attttcaata tatcttttgt
     4681 ttgtttcaat aatgtagaag aaaacctgcc catcttaata ttattgaatt gcttattaat
     4741 aagcttcaaa aaacgctcac gattctcttt atcaatacct gcatatatcg taaagagacc
     4801 tgtaaagctg tcaaattgac tacctatagt ataggctaaa ccttgctttt ctcgaatttc
     4861 tgtaaacaac aaggagtggg caaaagcacc aaataaaccg ttaaatacta ttaaagcaaa
     4921 ataatcttca tctttataag ttattggaag atgataagcc aattgcataa tagattgatt
     4981 aacgtcctta tcttctactt gacttctcgt tatattttca taagtctgcg tataatcaaa
     5041 agctaatact ttgtgacgtg gctcaaatgc catacgatta aaagcctgaa tcattcgata
     5101 gtcatcaaaa tcccctacga caaaaatatc taattgatct tccttaagca tcttctgaaa
     5161 ttcttgataa gctgtaaatg aattttctga ttccactaaa gatgctgtac catacttagg
     5221 taaacgaagt gtcttattat tgtagaataa agattttagt gctaagtcac tgctataaaa
     5281 attatcttca atatcagcct ctaaatattg aataaggttt ttcttctcag tatcaaaaat
     5341 agatgtttga tactgttcta aagaaattaa aggagaaaac agcatatcct ccaagaatgt
     5401 aataatttgt tcaacgatat tttcttgttc taaagtaaag gtatttttaa caaatacaat
     5461 atcaatatca acaatgtgaa caagaccctt agtagaaatc ttagttgata aactagcgcc
     5521 atataaggaa gctaactttt ctcgaaattc ttgtacctta ggatacttag catttgcagt
     5581 aactaacatt tgagcaacga gcgatctacg tgctactgtt ttattattaa aatcacctga
     5641 aaatcgaaaa gtaagatgat tagtcttaaa ttgttggttt ttaattaaat gtagagaaac
     5701 gccttcaact attttcattc aatatccttt gtgttctgta ttgttagcta agattatatc
     5761 atttttaaag gcgttaaaaa agattaaaca tccaagatat gattaattct aaatttcaat
     5821 cttcttcaaa attgacaaag tcgtcaacca atatttactc ccatttttta taccttgtct
     5881 ccatatcata agttcaactc aaaaaataat tttatgctat aatgatgtga tatctaaaat
     5941 tggagattaa aaagtcatgg attataaatt atttgatgaa tatattactt tacaatcact
     6001 tctaaaagag attggtatca ttcaaagtgg cggagccatt aaaaaatttt tagcggataa
     6061 tcgagtattg tttaacggtg accttgaaaa taggcgtgga aagaaactcc gcctggggga
     6121 tattattact attcctgatc aaaatatcga gatcattata aggaagccat cagatcaaga
     6181 aattgaagaa cgtaatatag aaatagctga aaaacagcgt gtttcagcaa tcgttaaaga
     6241 aatgaataaa aacaccaaca aaggtaaatc aaaaactagc aaaaaaccag ttcgctttcc
     6301 agggacataa aaatgtggat aaaaaatatt tctttaaaac attatcgtaa ttatgaagaa
     6361 gcacaagtag atttttcacc caacctcaat attttcattg gccgcaatgc tcaaggaaag
     6421 acaaattttt tagaagcaat ctacttcttg gctttgactc gaagtcaccg tacccgttct
     6481 gataaagaac ttgtacattt taaacaccat gatgtacaaa ttacaggtga ggtaatccgc
     6541 aaaagtggcc atcttaactt agatattcaa ttatctgaaa aaggaagaat cactaaagtt
     6601 aaccatctta aacaggcaaa attatctgat tatattgggg ctatgactgt cgtacttttt
     6661 gcccctgaag atcttcaatt agttaaaggt gcacctagtc taagacgaaa atttttagat
     6721 atagatattg gacaaattaa gccaacgtac ttagctgaac tttcaaatta caatcatgta
     6781 ttaaaacaaa gaaacactta tctaaaaact actaataacg tcgacaaaac ttttttaaca
     6841 gtccttgatg aacaattagc tgattatgga agtcgtgtca ttgaacaccg ttttgatttt
     6901 atccaagccc taaatgatga agcggacaaa catcactaca ttatcagtac tgagttagaa
     6961 catttatcta ttcactacaa atcgtcaata gagtttacag ataagtcaag tattagggaa
     7021 cattttttaa accaattatc taaaagccac tcaagagaca tctttaagaa aaacacaagc
     7081 atcggacctc atcgtgatga tattactttc tttattaatg atatcaatgc tacttttgct
     7141 agccaagggc aacaaagaag ccttatttta tcattgaaat tagcagaaat tgagctaata
     7201 aaaaccgtta ccaacgatta tccgattctc ttacttgacg acgtcatgag tgaacttgac
     7261 aaccatcgtc aactaaaact cttagaggga atcaaagaga atgttcaaac atttattact
     7321 acaacaagtt tagaacattt aagtgcttta cctgatcaac tcaaaatctt taacgttagt
     7381 gatggtacta ttagtattaa tgaaaaaaaa gcaacagatt aatctgttgc tttttaataa
     7441 gatttaacaa taccaagtaa tatagcaccc ataacaaaac aaaggatacc catgacaacc
     7501 cacttctgct ctttcttcgt ttttgtctca cctaaaaata aaataccacc aataatagag
     7561 ataattactc caagttgaga aaaactaaaa gcaattgcta gccctgcttt agctgctgcc
     7621 aataacatga agacattacc aacgccccac atgagacctg taatcatatt tttaacaaca
     7681 acagcctcaa agttaacacg aaacttcatg aaacaaattg cacctagaca cattccaaca
     7741 gccatgggta aaataacggc catagcgtcg aacttcataa tgttattaaa taaaactgcg
     7801 tatgagatgt aacctacagt tgaataagca attgtagcaa atccttttga aaactcttga
     7861 tgtgttgcca aagcttgttc tgagacatca cgtttacttg agaaatagaa gccgataact
     7921 aataatgtca acgctgtcaa tcctaaaata aattggattg gcttagtcca ttcatgaaag
     7981 actaaagcac caactaggct tccacctact aattgtgcac cacttgacag tggattagca
     8041 acagagactc ccatatattt cattgcttgg aattgaccat tttggccgac tgaccatagg
     8101 ataccaccta agataccaaa aatccacaat gaggcagtca tctctggctg tttaaataac
     8161 catacgataa tcgcaaatag caatgctcct aaagtcattc caaatgtttg ttgatttgga
     8221 cgtcctccaa ttttattact aacaaatcca atacttcccc acgcaaacat gggaatcaat
     8281 gcaataagta aaccttccaa ttctttctcc ttacaatgga atattaagta ttacctattt
     8341 tcaatcctag agaatctatt gactaacttt attagcaaga tcagcaaata ctatagcttt
     8401 atcaacacta tctttttcca aagctataat gacttgtttt atcaataaat tgaccagtga
     8461 tttagaataa ggtaactgtt ctttagtcat attactaatc ttatttaaaa taacatcttc
     8521 aggtaaaata tcttgaaaat agcagatgac tttgaaataa aaatcattgt actgtgtcac
     8581 accattcaga taagtaacta ccaggttaat ctcatcttga ggcagcctca tatttaaccc
     8641 taaatttgaa ataaaaagtt tagccaatat tttgagaaca ttgctttcta ttctatcaca
     8701 ggattgttgt ttttggaaac tgaaaattct catgatatct tcattatctc gataagattc
     8761 cagtaaagtt aattgctcta aatcagtatt agaagttcca gccttaactt ttgaatctag
     8821 aatggatact tcattgatag ttaccccaac ctccaacgtt tgtaaagctt taataaattt
     8881 atcaatacct atttgtgtct caccattttc aaaacgtgat agagttgatt ttgataattg
     8941 agcacttttg gctagagaac ttagggatat acctttgtcc tctctcaact cttttaaaat
     9001 ttttccataa tttttcatac aaaaacttta ctatatttgt ttcttttatg caacaaaaag
     9061 catcctcata ggggatgctt tttgccctta ttttagtgta ctgaataatt tggtgcctca
     9121 tttgtaattt gaacatcatg gggatggctt tctttcaagc cagctcctga catttcaaca
     9181 aattgagcat tatcatgaag ttcttttatg ttcgctgcac ctacatatcc cataccagag
     9241 cgaatgccac caagcatttg gaaaacaata tcggctactg aacctttgta tgctacacga
     9301 ccttcaatac cttctggaac caatttgtta gcctcgttta cagaaccttg gaaataacga
     9361 tcgcttgaac cttttttcat tgctgcaatt gagcccatac cacggtatgt cttgaactta
     9421 cgcccttgga aaatttctgt ttctcctggc gcttcatctg ttcctgcaaa cattgaacct
     9481 agcataactg catttccacc tgctgcaagt gcttttacaa tatcacctga atatttaata
     9541 ccaccatcag caatgatagt cttaccgtac tcacgcgcaa cagccgctgc atcgtaaata
     9601 gctgtaatct gaggaacacc taccccagca acgacacgag tcgtacaaat tgaacctgga
     9661 ccaataccaa ctttaacgac atccacacct gcatcataaa gtgcacgcgc tccttctgct
     9721 gtagcaatat ttcccgcaat caatgtgcga ttagggaagt gagcacgaat ttcagcaatt
     9781 ttacggagca caccagctga atgtccatgt gctgtatcaa tgacaatagc atctgcacct
     9841 gcttcaaaca aagcttctgc acgttcaaaa gtatcagagg ttacacctac tgcacctgca
     9901 actaagagac gtccaaattc atcttttgct gccttaggaa attcaataac tttttcgata
     9961 tccttaatag taataagtcc tgaaagacgt ctctcatcat ctactaaagg tagcttttca
    10021 atacgatgct catgaagaat acgctctgca gtttcaagat ctgtaccaat aggagctgtt
    10081 acaagatttt gacttgtcat gtgctctgag attagttgtt tataatcaga aatgaaacgc
    10141 atatcacggt tagtaataat tccaactaat ttacgattct cgagtgtctc tacaatagga
    10201 actccactaa tgcgatagtt ttgcatcaat tcctcagctt cagagacagt attatctggt
    10261 gtcaagaaga atgggtcaat aataacacca ttttctgagc gtttaacttt acgaacttct
    10321 tctgcttgat caactattga catattttta tgaatgatac caagcccacc agcacgtgca
    10381 atagcaatag ccatcttact atctgtaact gtatccattg cagctgtaat gattggaatg
    10441 tttaatgtca aattatcagc caactttgtc ttcatatcaa cttcatttgg taaaacatgg
    10501 ctttcagcag gaataagcag aacatcatca aaagtaaaac cttttttcaa aaacttagta
    10561 tcccaatttg acatcaaaaa atcctctttt cttagatagt agatataaaa aatcccatta
    10621 aaattatatt gttactatca tatcacgcta aaatcatttg tcaatataaa aagagcatag
    10681 aattaatagc ttctagctct ttaaagtaat gaaaaaatct tcttaatttt ctattttttt
    10741 gagaagaaga aaggcggcgt atactgactt aatttctcaa aacacttttg agcttcttca
    10801 ctattattac aaataatcaa acaagtatca tcaccacaga cagttgctaa gatctcaggt
    10861 aataaaatcg aatctaatat ggagccaaaa gattgagcta agccaggtag tgtcttcaaa
    10921 acgatttggt tttgaactgg aaacagcatg actagggcat cttccatgta aaaacgtaac
    10981 cgttcttccc aacgcttctg agatatacta tgaatctcat aataagtttc tgtagcatta
    11041 tcactgcttt cgttaacctt aattaaatta agttccttca tatctcgaga taatgttgct
    11101 tgcgtcactg aaacgccatt tttttctagt aattccctta actcatgttg tgtacgcacc
    11161 ttagtctcag agactaagga acgaattaat tggtgtcttg tttctttttt attcatataa
    11221 actccttatt caatgaattt cttgaaataa tttttattat tatagagaag cttctttcct
    11281 ttcatttcaa cgatatcttt ttggcgtaat tcctttaaaa catgactaac tgtctcgcga
    11341 gtagttccgc tcatattggc aatttcaatc gttgtaatag gaaaaggtaa atgtcccctt
    11401 tctgtaggaa cttgcaaaag tagagttgca agcgattgga taactctcat actggcactt
    11461 gaagtcacca tattacgaag tcgtatttca tgaactcgta atagcttaga atatttccga
    11521 caaagatgct tcatttgatt gatattttga agagagtatt cttcgtataa agccattggc
    11581 agagaaaaat actcaatatc cgtaatagcc actgctgaaa aatggtaatc atcatccaaa
    11641 aagagacctc cataaggaaa aaccgtacct tgtctaacat aatctgtata aatgaaagaa
    11701 ccagaagcgt ctgattgttc aatttttaca tgtcctgact ggattaaaaa cagtttttca
    11761 cggtaatcac cttcaaaaaa taaggtatga ttttttagag ctgtacgatg tttaatgtgg
    11821 ctcacaatat ggtcaaattg ttcaatagta aagtgtttaa aatcatctag ttgtctgaaa
    11881 taaaaatatt gttcttttgt aatcacctga ttttctagac ctttctaatc attagatgcg
    11941 aacatttttt gtataatgaa aattttttaa tgtataaaat tttcttgtca cgataaatcc
    12001 tccttcatta tagcaaaaaa attcgtagaa agaacgtttt tcgttgaatt ttgaaaacgc
    12061 ttttgagaaa atgtaattgt tctgatatct tttaaaaaaa ttgagaggcc ttcatgacta
    12121 aatttattgt tgattcatcg tactggaatt tgtttccaac ttctaaaatt ggagtaatct
    12181 taatcaaaga ttatcacatg gacagaaacc ttgaaacaga acttaaacag ttactgtctg
    12241 atagtcattc tttagcaaaa aaatatttgc aagagaaaga attcagtcaa aacagagtta
    12301 tccaaactta tcgcaaagca tatcaaacct ttaaaactaa aaaaggtgca cgttcaagta
    12361 tcgaagcatt attgaagcga gttaattcag gaaacgaaat tactagtata aatcctctag
    12421 ttgatatcta taatgccgct agccttaggt ttggattacc tattggtgct gaggatagtg
    12481 atacatttag aggtgattta aaactaacta ttaccaatgg tggtgatgaa ttttacttga
    12541 ttggcgaaga ttttaacaga ccaactcttt caggtgagct tgcctatgtt gatgatgttg
    12601 gagcagtttg ccgctgtttt aattggcgtg atggaaaacg tactatgatt accgataata
    12661 ctcagaatgc ttttttagtt atcgaattaa ttgataatgg aagagaaatt atttttaaag
    12721 aagcgttgga ctttatcgcc accaatacaa atcgttttct aaaagctaaa acacagacca
    12781 tcatattaga taaagaacac agtgaaataa cgctataaca ttaaaacgaa aagtaaaaat
    12841 tttacttttc gttatttttt taggttaata atttctcact ttccaacgat aaaagaaaga
    12901 aattgtatag aaatcgcttt catttctata aatttaaatt tttttggata taatcgtgac
    12961 attcttaaca cttttaatgc gaataacttc cctattttat attcaaaaga aagtagaatg
    13021 taaatgtaga tatagagata aatcctgttt caatacaaat ctctaactaa gaaaggagta
    13081 aaatatgact caaacacatc caattcatgt tttctcagaa attggaaaac ttaaaaaagt
    13141 tatgctacac cgtccaggta aagaaataga aaatctgatg cctgattatt tagagagact
    13201 cttgtttgat gatatcccat ttttggaaga tgcacaaaaa gaacacgatg cgtttgcaca
    13261 agcacttcgc aacgaaggag tcgaagtact ttaccttgaa aatcttgctg ccgagtcgtt
    13321 aacaaaccaa gaaattcgtg aacagtttat cgatgagtac attggcgaag caaatgttcg
    13381 cggtcgtgct actaaaaaag ctattcgaga attattactc aatatcaaag ataataaaga
    13441 acttatcgaa aaaactatgg caggtatcca aaagtctgag ctacctgaaa tcccatcttc
    13501 tgaaaaaggt ctaactgatc ttgtcgaatc taattatcca tttgccatag atccgatgcc
    13561 aaatctttac tttacacgtg atccatttgc aactattggg aatggagttt cattaaatca
    13621 tatgttctct gaaacacgta atcgcgaaac tttatacggt aaatatatct ttacacatca
    13681 cccagagtac ggtggaaaag tccctatggt ctatgaacgt gaagaaacaa ctcgtattga
    13741 aggtggagat gagcttgtcc tttctaaaga tgtccttgcc gttggtatct ctcaacgtac
    13801 tgatgctgcc tctattgaaa aactcttagt caatattttt aaacaaaatt taggttttaa
    13861 aaaggtttta gcatttgaat ttgcaaataa tcgtaaattt atgcaccttg acacagtctt
    13921 tacaatggtt gactatgaca agtttacaat tcacccagaa attgaaggcg atcttcgcgt
    13981 ttactctgta acttacgaaa accaagatct tcatattgaa gaagaaaaag gtgatttggc
    14041 agatcttcta gcgaaaaacc taggagttga gaaagtggaa ttgattcgtt gtgggggtga
    14101 caacttagtc gctgctggac gtgaacaatg gaatgatggt tcaaatacat taactatagc
    14161 accaggtgtc gttatcgttt acaacagaaa tacaatcaca aatgcaatct tagagtccaa
    14221 aggattgaaa ttaattaaga ttaatggaag tgaattagtt cgtgggcgtg gtggaccacg
    14281 ctgcatgtca atgccatttg aacgtgaaga cctttaaaat ttatgaagct attagtcaat
    14341 cacttgcctt accaaagagc tgcaagccta tatattcgct tttctgtttt cgttatcgaa
    14401 agaaatataa aaatggaaga agaattcgat gataatgacg agcaagatac tatttatgct
    14461 gttctttatg atgggaaaca gccagtttca acaggacgct ttcttcctga aactcaaaca
    14521 gaagctcgtt tgactcgaat tgcgacttta aaaggttatc gtggtaacgg ctatggtacc
    14581 aaaattatta tagcactaga aaattatgca aaagaaaatg gttatcacta tctaactata
    14641 catgctgaat tgacagcaaa agatttctac cagacacttg ggtatcaagc tactgggaat
    14701 atttatatgg aagacgggga agcctgtcaa acactcgaaa aatatttaat ttaaaggaga
    14761 cattaacata tgacacaagt atttcaagga cgtagtttct tagcagaaaa agatttttct
    14821 cgtgaggaat ttgaatatct tattgatttt tcagctcatt taaaagacct taaaaaacgt
    14881 ggtgttcctc atcattatct tgaaggtaaa aatattgctc tcttatttga aaaaacatct
    14941 actcgtactc gcgcagcctt tacaactgca gcaattgacc taggcgctca tccggaatac
    15001 cttggtgcaa atgatattca acttggtaaa aaagaatcaa cagaagatac tgctaaggtt
    15061 ttaggacgta tgtttgatgg tattgaattc cgtggtttta gccaaagaat ggttgaagag
    15121 cttgctgaat tttctggagt acctgtctgg aatggtttaa cagatgaatg gcatccaaca
    15181 caaatgctag ctgactacct tactatcaaa gaaaacttcg gtaaacttga aggtattact
    15241 cttgtttact gtggtgacgg acgtaacaat gttgccaact cgcttttagt ggctgggact
    15301 ttgatggggg tcaatgtaca catcttttct ccaaaagaac ttttccctgc tgaagagatt
    15361 gttaaattgg ctgaagaata tgccaaagag tccggggctc acgttctcgt tactgataat
    15421 gtagacgaag ctgtaaaggg agcagatgtc ttttacactg atgtctgggt atcgatggga
    15481 gaagaagata agttcaaaga acgcgttgaa cttcttcaac catatcaagt aaacatggaa
    15541 ctgattaaaa aagctaataa tgataatctt atcttcttac actgcttacc tgcattccat
    15601 gatacaaata ccgtttacgg caaagacgtc gctgaaaaat ttggcgtcaa ggaaatggaa
    15661 gttactgatg aagtcttccg tagcaaatat gctcgtcatt tcgaccaagc tgaaaatcgt
    15721 atgcacacta ttaaagctgt aatggctgca acccttggaa atcttttcat tccaaaagtt
    15781 taaattaaac gactataggg agttgacata tagtcaattc cctattttag gaaatgaggt
    15841 ataaaatgga aaaagaaaaa aaattaggtc ttttaccact aacaatgctt gtcattggct
    15901 ctcttatcgg tggcggaatc tttgatttaa tgcaaaatat gagttccaga gccggtttgg
    15961 taccaatgct tattgcttgg gtaattactg ctatcgggat ggggactttc gttttaagtt
    16021 ttcaaaattt atctgaaaaa aggccggacc taacagctgg aatctttagt tacgctaaag
    16081 aggggtttgg aaactttatg ggatttaact ctgcatgggg ttattggtta tcagcttggc
    16141 ttggaaatgt tgcctacgct gcactcttat tcagttcact cggttatttc tttaaattct
    16201 ttggtaatgg aaataatatc atctcaatta ttggagcaag tatagttatt tgggttgtcc
    16261 atttcttaat tttaagaggt gttaatacag ctgcatttat taataccata gttacctttg
    16321 caaaattagt acctgttatt attttcttaa tttcagcgtt attagctttc aaatttaaca
    16381 tttttagtct tgatatctgg ggaaatggat tacatcaatc aattttcaac caagtcaatt
    16441 caactatgaa aaccgctgtt tgggtattta ttggtattga gggcgccgtt gtcttctcag
    16501 gtcgtgctaa aaaacactct gatattggta aagcaagtat cctagcatta ttcactatga
    16561 tttcacttta tgtattgatt tctgttttat cacttggtat catgtcacgt ccagaacttg
    16621 caaacttaaa aacaccagct atggcttacg ttctagaaaa ggctgttggt cactggggcg
    16681 ctatcttagt taaccttggt gttatcattt cagtatttgg cgctattctt gcttggactt
    16741 tatttgcagc agaattacca tatcaagctg ctaaagaagg tgcttttcct aaattctttg
    16801 caaaagaaaa taaaaacaaa gctccaatca attcactctt agtcactaat ctttgtgtac
    16861 aagcattctt aatcacgttc ttattcacac aaagtgctta tcgttttggt ttcgcattag
    16921 catcatctgc tatcttaatt ccttatgctt ttacagcact atatcaatta caattcacac
    16981 tccgtgagga taagtcaact ccaggacatc aaaagaattt aattatcggt atcctcgcta
    17041 caatctatgc tgtttacctt atctacgctg gtggttttga ttacttactt ttgacaatga
    17101 ttgcttacac tctaggtatg attctctata ttaaaatgag aaaagatgac aagcttccta
    17161 tctttgtggg atatgaaaaa attagtgcca ttgtcattct agcgctttgc ttattatgta
    17221 ttattgaaat tatgactgga caaattgaca ttgctgcaat cctagcaggt tagtcattag
    17281 aaagagggat aaattggtaa aagaacatca aaaaatcgta gttgccttag gtggcaacgc
    17341 tattctatca acagatgctt cagcaaaagc acaacaagaa gcattaatca acacatcaaa
    17401 atcactggtc aaactgatta aagaaggcca tgatgtcatt gttactcatg gaaatggtcc
    17461 ccaagttggt aacttattgc tacagcaagc agcttcagat tctgaaaaga atccagcaat
    17521 gcctttggat acttgtgttg ccatgacaga aggaagtatt ggtttttggt tacaaaatgc
    17581 tcttaacaat gaattacaag aacaaggcat tgacaaagag gttgcaacag tggtaactca
    17641 agttattgta gatgaaaaag atcaagcttt cactaatcca acaaaaccta ttggcccatt
    17701 cttatctgaa gaagatgcca agaaacaagc ccaagaaaca ggttcaaaat ttaaagaaga
    17761 tgctggccgt ggatggcgta aagtcgttcc ttcaccaaaa ccggttggta tcaaagaagc
    17821 ttctgttatt agacggttag tggactctgg tgtggttgtt attagcgcag gtggcggagg
    17881 tgtaccagtt attgaagatg ctaatacaaa ggcattaaaa ggggttgaag ctgttattga
    17941 caaagacttt gccagtcaaa ctttatcaga acttgttgat gctgacttat tcatcgtctt
    18001 aactggcgtt gacaacgttt ttgttaattt taataaacct aatcaagaaa aattagaaga
    18061 agttactgtt tcacaaatga aacaatatat cactgaaaat caatttgccc ctggaagtat
    18121 gctaccaaag gtcgaagctg cgattgcttt tgttgaaaat aaacctgaat cacgagctat
    18181 tattacttca cttgaaaata ttgataatgt tttagcccaa aatgcaggga cacaaattgt
    18241 tgcaaattaa acaatgtaga ttaccattac atacactcct tattaatgtc actccttagg
    18301 cattcctata ataaaag
//
LOCUS       DBJYSH010000036        17804 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.036, whole genome shotgun sequence.
ACCESSION   DBJYSH010000036 DBJYSH010000000
VERSION     DBJYSH010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 17804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17804
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.036"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            67..276
                     /locus_tag="ACUBV3_000937"
     CDS             67..276
                     /locus_tag="ACUBV3_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HHU3847450.1"
                     /translation="MKVLFDVQNLLKKFGIYVYIGKRLYDIEVMKIELQRLYDNGLIS
                     RDDYLKAELILRREHRLELEKENKK"
     gene            273..1241
                     /locus_tag="ACUBV3_000938"
     CDS             273..1241
                     /locus_tag="ACUBV3_000938"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HHU3847451.1"
                     /translation="MSKKLLGIDLGGTTIKFGILTLEGEVQEKWAIETNTLENGRHIV
                     SDIVESLKHRLSLYGLTKDDFLGIGMGSPGAVDRTSKTVTGAFNLNWADTQEVGSVIE
                     KEVGIPFFIDNDANVAALGERWVGAGANNPDVVFVTLGTGVGGGVIADGNLIHGVAGA
                     GGEIGHMIVDPENGFMCTCGNKGCLETVASATGVVRVARQLAEQYEGSSAIKAAIDNG
                     DTVTSKDIFIAAEDGDKFANSVVERVSRYLGLAAANISNILNPDSVVIGGGVSAAGEF
                     LRSRVEKYFVTFAFPQVKKSTKIKIAELGNDAGIIGAASLANQQAS"
     gene            1253..1633
                     /locus_tag="ACUBV3_000939"
     CDS             1253..1633
                     /locus_tag="ACUBV3_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HHU3847452.1"
                     /translation="MDMSVILIIVILLAFVAWASWNYWRVRRAAKFLDNESFQKEMSR
                     GQLIDIREAGAFHRKHILGARNIPASQFKVALSALRKDKPVLLYDASRGQSIPRIVLL
                     LRKEGFNQLYVLKDGFNYWTGRVK"
     gene            1865..3706
                     /gene="typA"
                     /locus_tag="ACUBV3_000940"
     CDS             1865..3706
                     /gene="typA"
                     /locus_tag="ACUBV3_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018381299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HHU3847453.1"
                     /translation="MTNLRTDIRNVAIIAHVDHGKTTLVDELLKQSHTLDERKELEER
                     AMDSNDIEKERGITILAKNTAVAYNDVRINIMDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQNLIPIVVVNKIDKPSARPSEVVDEVLELFIELGADDDQ
                     LDFPVVYASAINGTSSMSDDPSDQEKTMAPIFDTIIDHIPAPVDNSEEPLQFQVSLLD
                     YNDFVGRIGIGRVFRGTVKVGDQVTLSKLDGTTKNFRVTKLFGFFGLERKEIQEAKAG
                     DLIAVSGMEDIFVGETVTPTDAIEPLPVLRIDEPTLQMTFLVNNSPFAGREGKWITSR
                     KVEERLLAELQTDVSLRVDPTDSPDKWTVSGRGELHLSILIETMRREGYELQVSRPEV
                     IIKEIDGVQCEPFERVQIDTPEEYQGAIIQSLSERKGDMLDMQMVGNGQTRLIFLIPA
                     RGLIGYSTEFLSMTRGYGIMNHTFDQYLPVVQGEIGGRHRGALVSIENGKATTYSIMR
                     IEERGTIFVNPGTEVYEGMIVGENSRDNDLGVNITTAKQMTNVRSATKDQTAVIKTPR
                     ILTLEESLEFLADDEYMEVTPESIRLRKQILNKAARDKANKKKKSAE"
     gene            3751..3996
                     /locus_tag="ACUBV3_000941"
     CDS             3751..3996
                     /locus_tag="ACUBV3_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3165 family protein"
                     /protein_id="HHU3847454.1"
                     /translation="MFYLIVALLIALYYFFMAPKTVRNTLNAIGLVGLVALLLVLAVM
                     SFIKILQLPGELYIGLIMIPLGYTAFKEILNLSEKKK"
     gene            4126..5481
                     /gene="murD"
                     /locus_tag="ACUBV3_000942"
     CDS             4126..5481
                     /gene="murD"
                     /locus_tag="ACUBV3_000942"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HHU3847455.1"
                     /translation="MKTITTFENKKVLVLGLARSGEAAARLLAKLGAIVTVNDGKPFD
                     ENPTAQSLLEEGIKVVCGSHPLELLDEDFCYMIKNPGIPYNNPMVKKALEKQIPVLTE
                     VELAYLVSESQLIGITGSNGKTTTTTMIAEVLNAGGQRGLLAGNIGFPASEVVQAADD
                     KDILVMELSSFQLMGVKEFRPHIAVITNLMPTHLDYHGSFEDYVAAKWNIQNQMSSSD
                     FLVLNFNQGISKELAKTTKATIVPFSTTEKVDGAYVQDKQLFYKGENIMLVDDIGVPG
                     SHNVENALATIAVAKLAGISNQVIRETLSNFGGVKHRLQSLGKVHGISFYNDSKSTNI
                     LATQKALSGFDNTKVILIAGGLDRGNEFDELIPDITGLKYMVVLGESASRVKRAAQKA
                     GVTYSDALDVRDAVHKAYEVAQQGDVILLSPANASWDMYKNFEVRGDEFIDTFESLRG
                     E"
     gene            5484..6560
                     /locus_tag="ACUBV3_000943"
     CDS             5484..6560
                     /locus_tag="ACUBV3_000943"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0051991 -
                     UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-
                     glutamyl-meso-2,
                     6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol
                     4-beta-N-acetylglucosaminlytransferase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HHU3847456.1"
                     /translation="MGKKIVFTGGGTVGHVTLNLILIPKFIKDGWEVHYIGDKNGIEH
                     EQINQSGLDITFHSIATGKLRRYFSWQNMLDVFKVGVGVLQSIAIIAKLRPQALFSKG
                     GFVSVPPVVAARLLKVPVFVHESDLSMGLANKIAYKFATIMYTTFEQSKDLIKTKHIG
                     AVTKVMDCKKSFENTDLTSIKEAFDPNLKTLLFIGGSAGAKVFNDFITQTPELEEKYN
                     VINISGDSSLNRLKKNLYRVDYVTDLYQPLMNLADVVVTRGGSNTIFELVAMKKLHLI
                     IPLGREASRGDQLENAAYFEEKGYALQLPESELNINTLEKQINLLISNSESYEKNMSQ
                     SSEIKSQDEFYQLLIDDMAKVTKG"
     gene            6564..7700
                     /locus_tag="ACUBV3_000944"
     CDS             6564..7700
                     /locus_tag="ACUBV3_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001124859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HHU3847457.1"
                     /translation="MPKKKSDTPEKEEVVLTEWQKRNLEFLKKRKEDEEEQKRINEKL
                     RLDKRSKLNISSPEEPQNTTKIKKLHFPKISKPKIEKKQKKEKIVNSLAKTNRIRTAP
                     IFVVAFLVILVSVFLLTPFSKQKTITVSGNQHTPDDILIEKTNIQKNDYFFSLIFKHK
                     AIEQRLAAEDVWVKTAQMTYQFPNKFHIQVQENKIIAYAHTKQGYQPVLETGKKADPV
                     NSSELPKHFLTINLDKEDSIKLLIKDLKALDPDLISEIQVISLADSKTTPDLLLLDMH
                     DGNSIRIPLSKFKERLPFYKQIKKNLKEPSIVDMEVGVYTTTSTIESTPVKAEDTKNK
                     STDKTQTQNGQVAENSQGQTNNSNTNQQGQQIATEQAPNPQNVN"
     gene            7973..9166
                     /gene="ftsA"
                     /locus_tag="ACUBV3_000945"
                     /pseudo
     CDS             7973..9166
                     /gene="ftsA"
                     /locus_tag="ACUBV3_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983860.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
     gene            9140..9346
                     /locus_tag="ACUBV3_000946"
     CDS             9140..9346
                     /locus_tag="ACUBV3_000946"
                     /inference="COORDINATES: protein motif:HMM:NF023409.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA C-terminal
                     domain-containing protein"
                     /protein_id="HHU3847458.1"
                     /translation="MLRHKPVDFDYKEKTNTMSTMPYSEPLTSSMEDSNLEPIRAREN
                     AQEPTEPKANIGERIRGIFGSMFD"
     gene            9368..10648
                     /gene="ftsZ"
                     /locus_tag="ACUBV3_000947"
     CDS             9368..10648
                     /gene="ftsZ"
                     /locus_tag="ACUBV3_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HHU3847459.1"
                     /translation="MVFSFDTASVQGAVIKVIGVGGGGGNAINRMIDEGVAGVEFIAA
                     NTDIQALSSSKAETVIQLGPKLTRGLGAGGQPEVGRKAAEESEEVLTEALTGADMVFI
                     TAGMGGGSGTGAAPVIARIAKSLGALTVAVITRPFGFEGNKRSNFAIEGIQELREQVD
                     TLLIISNNNLLEIVDKKTPLLEALSEADNVLRQGVQGITDLITNPGLINLDFADVKTV
                     MANKGNALMGIGIGSGEERITEAARKAIYSPLLETTIDGAEDVIVNVTGGMDMTLTEA
                     EEASEIVSQAAGKGVNIWLGTSIDMDMKDEIRVTVVATGVRKDKTNQVSGFTTSAPTN
                     QAPSERQSTSNSNFDRRGNFDMTESREMPTQQNQPHTQNQQQSSAFGNWDLRRDNISR
                     PTEGELDSKLSMSTFSENDDMDDELETPPFFKNR"
     gene            10654..11328
                     /locus_tag="ACUBV3_000948"
     CDS             10654..11328
                     /locus_tag="ACUBV3_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000981484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HHU3847460.1"
                     /translation="MMNLQENKTAIFDNVSKLALKAGRAPESVHVVAVTKYVNCQTTE
                     ALIRTGVNHIGENRVDKFLEKYQALKDEKLTWHLIGSLQRRKVKDVINYVDYFHALDS
                     VKLAAEIQKRAQKPVKCFLQVNISREDSKHGFTIEQIDDALNLISRYDKIELIGIMTM
                     APLKATKEEISSIFEETESLRKRLQARNIERMPFTELSMGMSRDYDIAIQNGSTFVRI
                     GTSFFK"
     gene            11340..11945
                     /locus_tag="ACUBV3_000949"
     CDS             11340..11945
                     /locus_tag="ACUBV3_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA];
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HHU3847461.1"
                     /translation="MALKDRFDKIISYFDTDDVSENEVHEVQERTSVQRDSRAATAQE
                     ASQRSHMTNSAEEEMIGSRPRTSTYNPNRQERQRVQRDNAYQQATPRIQNKDSVRQQR
                     EQVTIALKYPRKYEDAQEIVDLLIVNECVLIDFQYMLDAQARRCLDYIDGASRVLYGS
                     LQKVGSSMFLLTPANVMVDIEEMNIPKTGQETSFDFDMKRR"
     gene            11948..12202
                     /locus_tag="ACUBV3_000950"
     CDS             11948..12202
                     /locus_tag="ACUBV3_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HHU3847462.1"
                     /translation="MILVLLILLKTIRVYSYILLAYALLSWFPGAYDSWIGRLVVQIV
                     DPIIRPLRRFNLQFLGLDFTVIVAFFLLNMLSRFLILLFT"
     gene            12204..12992
                     /locus_tag="ACUBV3_000951"
     CDS             12204..12992
                     /locus_tag="ACUBV3_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HHU3847463.1"
                     /translation="MTLDDIYQHFRPEEYAFINKIDHLAQYVENTYSFITTEFLNPRE
                     FKILESVLERRGSHYYTSGQYFQTEYVKVIIAPEYYQLDMVDFNLSLIEIKYNAKFNH
                     LTHAKIMGTLLNYLGVKRSILGDILVEEGCAQVLVDSQMTNHLVHSVTKIGTASVQLA
                     EVPLSKLLTPKQDIQKLTVIASSLRLDKILATILKISRTQSTKLIEADKVKVNYAIVN
                     RVSEQLVEGDLISVRGYGRFTLNHNLGLTKNQKYKLEVDKMIHN"
     gene            13002..13772
                     /locus_tag="ACUBV3_000952"
     CDS             13002..13772
                     /locus_tag="ACUBV3_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HHU3847464.1"
                     /translation="MPLTALEIKDKTFSSKFRGYSEEEVNEFLEIVVDDYEDLIRRNR
                     EQEQYIKDLEEKIAYFNEMKESLSQSVILAQETAERVKISAQDEASNLMGKATFDAQH
                     LIDEAKLKANQILRDATDDAKRVAIETEDLKRQSRVFHQRLLSELEGQLKLANSSAWE
                     ELLKPTAIYLQNSDASFKEVVEKVLDEDDALPVVDDTESFDATRQFSPDEMEELQRRV
                     EESNKQLEESGLLDTNNFQMEEPINLGETQTFKLNIED"
     gene            14057..16849
                     /gene="ileS"
                     /locus_tag="ACUBV3_000953"
     CDS             14057..16849
                     /gene="ileS"
                     /locus_tag="ACUBV3_000953"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HHU3847465.1"
                     /translation="MKLKETLNLGQTAFPMRAGLPNKEPQWQEAWDQADIYKKRQALN
                     EGKPAFHLHDGPPYANGNIHVGHALNKISKDIIVRSKSMSGFRAPYVPGWDTHGLPIE
                     QVLAKKGVKRKEMDLAEYLEMCRDYALSQVDKQRDDFKRLGVSADWENPYITLTPDYE
                     ADQVRVFGAMADKGYIYRGAKPVYWSWSSESALAEAEIEYHDIDSTSLYYANKVKDGK
                     GILDTDTYIVVWTTTPFTVTASRGLTVGPDMEYVVVAPVGSERKYLLAEVLVDSLAAK
                     FGWENFEIVTHHTGKELNHIVTEHPWDTEVEELVILGDHVTTDSGTGIVHTAPGFGED
                     DYNVGIANGLDVVVTVDSRGLMMENAGPDFEGQFYDKVTPLVKEKLGDLLLASEVINH
                     SYPFDWRTKKPIIWRAVPQWFASVSKFRQEILDEIEKTNFQPEWGKKRLYNMIRDRGD
                     WVISRQRAWGVPLPIFYAEDGTAIMTKEVTDHVADLFAEYGSIVWWQRDAKDLLPAGY
                     THPGSPNGLFEKETDIMDVWFDSGSSWNGVMNARENLSYPADLYLEGSDQYRGWFNSS
                     LITSVAVNGHAPYKAVLSQGFVLDGKGEKMSKSLGNTILPSDVEKQFGAEILRLWVTS
                     VDSSNDVRISMDILKQTSETYRKIRNTLRFLIANTSDFNPKQDAVAYENLGAVDRYMT
                     IKFNQVVDTINKAYAAYDFMAIYKAVVNFVTVDLSAFYLDFAKDVVYIEAANSPERRR
                     MQTVFYDILVKLTKLLTPILPHTAEEIWSYLEHEEEEFVQLAEMPVAQTFSGQEEILE
                     EWSAFMTLRTQAQKALEEARNAKVIGKSLEAHLTIYASQEVKTLLTALNSDIALLMIV
                     SQLTIADEADKPADSVSFEGVAFTVEHAEGEVCERSRRIDPTTKMRSYGVAVCDASAA
                     IIEQYYPEAVAQGFEA"
     gene            complement(16966..17268)
                     /locus_tag="ACUBV3_000954"
     CDS             complement(16966..17268)
                     /locus_tag="ACUBV3_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HHU3847466.1"
                     /translation="MRLINTTSSHPELVRNQLQNTDAKLVEVYSAGNTDVVFTKAPKH
                     YELLISNKYRAIKDEELEAIREFFLKRKIDQSIIIQEQMKSLHTAKLIEISYPTTA"
     gene            complement(17332..17787)
                     /locus_tag="ACUBV3_000955"
     CDS             complement(17332..17787)
                     /locus_tag="ACUBV3_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HHU3847467.1"
                     /translation="MTNPTFGEKIDNVDYRSRFGVYAIIPNPTHDKIILVQAPNGAWF
                     LPGGEIEENENHLEALTRELIEELGYSATIGHYYGQADEYFYSRHRDTYYYNPAYIYE
                     VTAYHKEQAPLEDFNHLAWFPIQEAKEKLKRGSHRWGVQAWEKNHHSRK"
BASE COUNT         5920 a         2826 c         3604 g         5454 t
ORIGIN      
        1 aatgacaatt agcaagcggt tgcggatttt cttgaaaatt gaatggtttt ctgttaaaat
       61 tcatatatga aagttttatt cgatgtgcag aatcttttga aaaaatttgg tatttatgtt
      121 tatattggta aacgtcttta tgatatcgaa gttatgaaga ttgaattgca acgtctctac
      181 gataatggtc tgataagtag agatgattat ttaaaagcag aactgatttt aagaagagag
      241 cacagactag aattggagaa ggaaaataaa aaatgagtaa gaaattattg ggaattgacc
      301 tcggaggaac gaccattaaa tttggtatct tgacgcttga gggagaagta caagaaaaat
      361 gggcaattga gaccaatact ttagaaaacg gaagacatat cgtttctgat atcgttgaat
      421 ctctcaaaca tcgtttgagc ctctatggat taacaaaaga tgactttctc ggtatcggta
      481 tggggtctcc aggagctgtt gatagaacta gtaaaacagt aacaggtgct tttaatctaa
      541 attgggctga tactcaagaa gtaggttcag ttattgaaaa agaagttgga attccatttt
      601 ttattgataa cgatgctaat gttgcagcac ttggtgaacg ctgggtaggt gctggtgcca
      661 ataatcccga cgttgttttc gtaaccctcg gaacaggagt aggtggaggt gttatcgcag
      721 atggtaacct catccatggt gttgcaggag caggtggaga aattgggcat atgattgttg
      781 atccagaaaa tggatttatg tgcacatgtg gtaacaaagg ctgtcttgag acagttgcat
      841 cagcgacagg tgttgttaga gtagcacgtc aactcgcaga acagtatgaa ggttcgtctg
      901 ccattaaagc agcgattgac aacggtgata ctgttacaag taaagatatt tttatagcag
      961 cagaagatgg ggataaattt gctaattctg ttgttgaacg tgtatcacgt taccttggac
     1021 tggcagcagc taatatttca aatattttaa accctgattc tgtggttatt ggtggcggtg
     1081 tctcagcagc aggtgaattt ttacgtagtc gcgttgagaa atactttgtc acatttgctt
     1141 tcccacaagt taaaaagtca actaaaatta agattgctga actaggtaat gatgctggta
     1201 ttattggtgc agcaagctta gccaatcaac aagcaagtta aggagataag atatggatat
     1261 gtctgttatt ttaattatcg ttattcttct ggcttttgtt gcttgggcat cttggaatta
     1321 ttggagggtt cgtagggcag ctaaattttt agataacgag tcttttcaaa aagaaatgtc
     1381 acgaggacag ttaattgata tccgagaagc tggcgctttt catagaaaac atattctcgg
     1441 agcacgtaac attccagcaa gccaatttaa agttgcctta tcggctttac gcaaagataa
     1501 acctgtcctc ttatatgacg caagccgtgg tcaatctatt ccaagaattg ttttgttact
     1561 cagaaaagaa ggttttaatc aactttacgt attaaaggac ggtttcaatt actggacggg
     1621 tagagttaag taatatacta aggagagagt tactattaat aactctctcc tctttttgta
     1681 gaaatgaatc aaattgaaaa aagtagaaaa taaatgacat aaataatgaa atgatgaaaa
     1741 gttttcttat cacaaacagg cagttgatat gaaaacattt acacttgtgt aaattctgtt
     1801 ttttaagaaa aattgtgtta taattcataa gttaacagaa ttacattata aaatagagga
     1861 aaacatgaca aatttaagaa cagatatccg taacgttgcg atcattgccc acgttgacca
     1921 cggtaaaaca actctcgttg atgaattatt aaaacaatca catactcttg atgagcgtaa
     1981 agagcttgaa gaacgtgcaa tggattcaaa tgatatcgaa aaagaacgtg gtatcaccat
     2041 tcttgcaaaa aatacagccg tagcatacaa cgatgttcgt atcaatatta tggacacacc
     2101 tggtcacgcg gactttggtg gtgaagttga gcgtattatg aaaatggttg atggtgttgt
     2161 tttagtcgtt gatgcctacg aaggaacaat gccacaaaca cgttttgttt tgaagaaagc
     2221 tcttgaacaa aacttaattc caatcgttgt tgtaaataaa attgataagc cgtcagctcg
     2281 tccatcagag gttgttgatg aagttcttga actctttatt gagctcggtg ctgatgatga
     2341 tcaactagat ttccctgttg tttatgcttc agctatcaat ggaacatctt caatgtcaga
     2401 tgatccttca gatcaagaaa aaacaatggc accgattttt gatactatca ttgatcacat
     2461 tccagcccca gttgacaact cggaagaacc acttcaattc caagtttctc ttcttgacta
     2521 caatgatttt gtaggacgta ttggtattgg acgtgttttc cgcgggactg tcaaagttgg
     2581 agatcaagtt actctttcaa aacttgatgg tacaactaaa aacttccgcg taacaaaact
     2641 ttttggtttc tttggacttg aacgtaaaga aatccaagag gctaaagcgg gtgatttaat
     2701 cgctgtttct ggtatggaag atatcttcgt tggtgagaca gtaactccga cagatgctat
     2761 tgaaccactt ccagttttac gtattgacga gccaacactt caaatgactt tcttggtgaa
     2821 taattcacca tttgcaggtc gcgaaggtaa atggattacg tcacgtaagg ttgaagaacg
     2881 tcttttagca gaattacaaa cagacgtttc tttgcgtgtt gacccaacag attcgccaga
     2941 taaatggacg gtttcagggc gtggagaatt acatttatct atccttattg aaacaatgcg
     3001 tcgtgaggga tatgaacttc aagtatcacg tccagaagtt atcatcaaag aaattgatgg
     3061 tgttcaatgc gagccgtttg agcgtgttca aattgatact ccagaagaat atcagggtgc
     3121 tattatccaa agtttgtcag agcgtaaagg tgatatgctt gatatgcaga tggttggtaa
     3181 tggtcaaacg cgtttgattt tcttgattcc tgcacgtggt ttgattggtt attcaacaga
     3241 gtttctttca atgacacgtg gatatggtat catgaatcat acttttgacc agtatctacc
     3301 ggttgttcaa ggagaaattg gtggccgtca tcgtggtgcc ttggtttcta ttgaaaatgg
     3361 taaagcaact acatattcaa ttatgcgtat tgaagagcgt ggaactatct ttgtaaatcc
     3421 aggtacagaa gtttatgaag gaatgattgt tggtgagaat tctcgtgata atgacctcgg
     3481 agtcaatatt acaactgcta aacaaatgac aaatgtccgt tcagcaacta aagatcaaac
     3541 tgcagtcatc aagacgcctc gtatccttac tttagaagag tcacttgaat ttttagcaga
     3601 tgatgagtac atggaagtaa ctcctgagtc tattcgtctt cgtaaacaaa ttttaaataa
     3661 agcagcacgt gacaaagcta ataaaaagaa aaaatcagct gaataatatc caaagtattt
     3721 cttatttatg attaataaga aaggagttct atgttttact taattgttgc ccttttgatt
     3781 gccttatatt acttttttat ggcaccaaaa acagtacgta atactttgaa cgctattggt
     3841 ttagtaggtt tggtagcctt attacttgtc ttggcagtta tgagttttat taaaatcctt
     3901 caattgccag gtgaattgta tattgggctt ataatgattc ctctaggata tacagcattt
     3961 aaagaaatat tgaatttgtc agaaaagaag aaatagctta tgaacttagg attttgtctc
     4021 ctaagttctt ttgttttgag aaattagaga catatccttt accttgaaaa agccactgat
     4081 taaaggtaaa ataataggga taataatgat agaatgggac gagtaatgaa aacaataaca
     4141 acatttgaaa ataaaaaagt tttagtcctt ggtttagcac gatctggaga agccgctgca
     4201 cgtttgttag ctaagttagg agcaatagtg acagttaatg atggcaaacc atttgatgaa
     4261 aatccaacag cacagtcttt gttggaagag ggtattaaag tggtttgtgg tagtcatcct
     4321 ttagaattgt tagatgagga tttttgttac atgattaaaa atccaggaat accttataac
     4381 aatcctatgg tcaaaaaagc attagaaaaa caaatccctg ttttgactga agtggaatta
     4441 gcatacttag tttcagaatc tcagctaata ggtattacag gctctaacgg gaaaacgaca
     4501 acgacaacga tgattgcaga agtcttaaat gctggaggtc agagaggttt gttagctggg
     4561 aatatcggct ttcctgctag tgaagttgtt caggctgcgg atgataaaga tattctagtt
     4621 atggaattat caagttttca gctaatggga gttaaggaat ttcgtcctca tattgcagta
     4681 attactaatt taatgccaac tcatttagat tatcatgggt cttttgaaga ttatgttgct
     4741 gcaaaatgga atatccaaaa tcaaatgtct tcatctgatt ttttggtact taattttaat
     4801 caaggtattt ctaaagagtt agctaaaact actaaagcaa caatcgttcc tttctctact
     4861 acggaaaaag ttgatggtgc ttacgtacaa gacaagcaac ttttctataa aggggagaat
     4921 attatgttag tagatgacat tggtgtccca ggaagccata acgtagagaa tgctctagca
     4981 actattgcgg ttgctaaact agctggtatc agtaatcaag ttattagaga aactttaagc
     5041 aattttggag gtgttaaaca ccgcttgcaa tcactcggta aggttcatgg tattagtttc
     5101 tataacgaca gcaagtcaac taatatattg gcaactcaaa aagcattatc tggctttgat
     5161 aatactaaag ttatcctaat tgcaggaggt cttgatcgcg gtaatgagtt tgatgaattg
     5221 ataccagata tcactggact taaatatatg gttgttttag gggaatcggc atctcgagta
     5281 aaacgtgctg cacaaaaagc aggagtaact tatagcgatg ctttagatgt tagagatgcg
     5341 gtacataaag cttatgaggt ggcacaacag ggcgatgtta tcttgctaag tcctgcaaat
     5401 gcatcatggg acatgtataa gaatttcgaa gtccgtggtg atgaattcat tgatactttc
     5461 gaaagtctta gaggagagta actatgggga aaaaaattgt ttttacaggt ggtggtacgg
     5521 taggtcatgt aacactaaac ctaatattaa tccccaaatt tatcaaagat ggttgggaag
     5581 tacattatat tggtgataaa aatggcatag aacatgaaca aattaatcag tctggacttg
     5641 atatcacatt tcactctata gctactggga aactacgtcg ctatttttca tggcaaaata
     5701 tgttagatgt ctttaaagta ggggttggcg ttcttcaatc gattgctatt attgctaaac
     5761 ttagacccca agccttattt tcaaaagggg gatttgtttc agtacctcct gtagtagctg
     5821 cacgcctatt aaaagttcct gtttttgttc atgaatcaga tttatcaatg gggttagcaa
     5881 ataagattgc ttataaattt gcgactatta tgtatacgac ttttgaacaa tctaaagatt
     5941 taatcaaaac taagcatatt ggtgctgtaa caaaagtcat ggattgtaaa aagtcgtttg
     6001 aaaatactga cttaactagt ataaaagaag ccttcgatcc aaacctaaag accttactat
     6061 ttattggagg atcagcaggt gcaaaagtat tcaatgattt tattactcaa acacctgagc
     6121 tggaagaaaa atataatgtt atcaatattt caggcgattc ttctctcaat cgattgaaga
     6181 agaaccttta tcgcgtggat tatgttacag acctttatca gcctttaatg aatttagcag
     6241 atgtggttgt tacacgaggt ggctctaata ctatttttga attagtagct atgaaaaaac
     6301 ttcatcttat tatccctctt ggacgtgaag cgagccgagg tgaccaatta gagaatgctg
     6361 cttattttga agaaaagggc tacgcattgc agttaccaga atctgaatta aacataaata
     6421 cacttgagaa acagataaat ttattaattt ctaacagtga gagttacgaa aaaaacatgt
     6481 cacaatcatc tgaaataaaa tctcaagatg aattttatca attattaatt gatgatatgg
     6541 caaaagtaac aaaaggataa aacatgccta agaagaaatc agatacccca gaaaaagaag
     6601 aagttgtctt aacggaatgg caaaagcgta accttgaatt tttaaaaaaa cgcaaagaag
     6661 atgaagaaga acaaaaacgt attaacgaaa aattacgctt agataaaaga agtaaattaa
     6721 atatttcttc tcctgaagaa cctcaaaata ctactaaaat taagaagctt cattttccaa
     6781 agatttcaaa acctaagatt gaaaagaaac agaaaaaaga aaaaatagtc aacagcttag
     6841 ccaaaactaa tcgcattaga actgcaccta tatttgtagt agcattccta gtcattttag
     6901 tttccgtttt cctactaact ccttttagta agcaaaaaac aataacagtt agtggaaatc
     6961 agcatacacc tgatgatatt ttgatagaaa aaacgaatat tcaaaaaaac gattatttct
     7021 tttctttaat ttttaaacat aaagctattg aacaacgttt agctgcagaa gatgtatggg
     7081 taaaaacagc tcagatgact tatcaatttc ccaataagtt tcatattcaa gttcaagaaa
     7141 ataagattat tgcatatgca catacaaagc aaggatatca gcctgtcttg gaaactggaa
     7201 aaaaggctga tcctgtaaat agttcagagc taccaaagca cttcttaaca attaaccttg
     7261 ataaggaaga tagtattaag ctattaatta aagatttaaa ggctttagac cctgatttaa
     7321 taagtgagat tcaggtgata agtttagctg attctaaaac gacacctgac ctcctgctgt
     7381 tagatatgca tgatggaaat agtattagaa taccattatc taaatttaaa gaaagacttc
     7441 ctttttacaa acaaattaag aagaacctta aggaaccttc tattgttgat atggaagtgg
     7501 gagtttacac aacaacaagt actattgaat caacccctgt gaaagcggaa gatacaaaaa
     7561 ataaatcaac tgataaaaca caaacacaaa atggtcaggt tgcggaaaat agtcaaggac
     7621 aaacaaataa ctcaaatact aatcaacaag gacaacagat agcaacagag caggcaccca
     7681 accctcaaaa tgttaattag cgataaaaaa agactggatt tatcagtctt ttttatacat
     7741 atcaagtcag tcatagattg ctagtaaaaa ttgatttctt gaaaaaaatt taagtttgtt
     7801 ataagttgcg aatggtttaa ataagttaga aatatttcaa tttgatgttg cttaataaaa
     7861 aaacgtaatt tggtaacaat tctgcttttt ttatggtata atattttctg aataattctg
     7921 tttttggcag atgtagaata gaaaagaatg gaaagatagc gaggtcgagt gaatggctag
     7981 aaatggcttt tttactggtt tggatatagg aacaagctcg attaaagttt tagttgcaga
     8041 atttattgca aatgaaatga atgtaattgg ggttagcaac gtccctagtt ccggtgtaaa
     8101 agatggtata attattgata ttgaggcagc agcaactgca atcaaagagg cggtaaaaca
     8161 agctgaggag aaagcaggca tcaccattga caaaatcaat gttggattac cagcaaatct
     8221 tctccaaatt gaaccaactc aaggaatgat tcctgttccc aatgaatcaa aagaaattaa
     8281 ggatgaagat gtcgaaagtg ttgttaaatc agcattgact aaaagcatta ctcctgaaag
     8341 agaagttatt tcattgattc cgcttgaatt cattgtagat ggtttccaag ggattagaga
     8401 ccctagaggg atgatgggga ttcgccttga gatgcgtggt cttatctata ctggaccaac
     8461 aactatcctt cataatcttc gtaaaactgt tgaacgagca ggtataaaag tagagcatgt
     8521 tgtgattgcc cctctagcct tagcaaaatc agttctaaac gaaggtgaac gtgagtttgg
     8581 tgcaactgtg attgatatgg gaggcggaca aacaacagtc gcttctatgc gtaatcaaga
     8641 attgcaatat actaatattt attctgaagg tagcgattat gtcaccaaag atatttctaa
     8701 agtgttacgt acaacagtag aaatcgccga agcattgaaa ttcaactttg gacaagctaa
     8761 tgttgaagaa gcaagtactt ctgatacagt tcaagttaat gttgttggta acgaggaacc
     8821 tgttgaaatt acagaaagct atctttcgca aattatttca ggacgtattc gtcaaatttt
     8881 agaacatgtc aaacaagact tgggtagagg tcgtttactt gatttacctg gtggtattat
     8941 tcttgttgga ggtggtgcaa ttatgccagg agttgtcgag gttgctcaac aaatttttgg
     9001 aactagagtt aaattgcacg ttccaaacca agtaggtatt cgcaatccaa tgttcgctaa
     9061 tgttattagt atcgttgatt atgtaggaat gatgtcagaa gttgacatta tcgcacaaca
     9121 tgctgtaact ggcgatgaaa tgctaagaca taaaccagtc gactttgatt ataaagaaaa
     9181 aacaaataca atgtcgacaa tgccttattc tgagccgtta acctcttcta tggaagattc
     9241 taatttagaa ccaatccgag caagagaaaa cgctcaagag cctactgagc ctaaagctaa
     9301 tattggtgag cgcatcagag gaatttttgg aagtatgttt gactaaaaca ataagtgagg
     9361 aataaaaatg gtattttcat ttgatacagc atcagtacaa ggtgcagtta ttaaagttat
     9421 tggtgttgga ggcggtggcg gtaacgctat taaccgcatg attgacgaag gtgtcgctgg
     9481 cgttgagttt attgcagcaa atactgatat tcaagcctta agtagctcaa aagctgaaac
     9541 tgtcattcaa cttggtccaa aattaactcg cggacttggt gcaggaggac aacctgaagt
     9601 tggtcgtaaa gcagctgaag aaagtgaaga agttttaaca gaagcactta ctggcgctga
     9661 tatggtattt atcacagcag gtatgggggg cggatctggt acaggtgcgg ctccagttat
     9721 tgcacgtatt gcaaagagtt taggagcact tacagtagcg gttatcacac gtccttttgg
     9781 ctttgagggt aataaacgtt ctaacttcgc tatcgaaggt attcaagaat taagagaaca
     9841 agttgataca ctgcttatca tttcaaataa taatcttctt gaaattgtag ataagaagac
     9901 accattgctt gaagctctta gtgaagcaga taatgtttta cgtcaaggtg ttcaaggtat
     9961 tacagatttg attactaacc caggtttaat taaccttgac tttgcagatg ttaaaacagt
    10021 tatggcaaat aaaggtaatg cactgatggg aattggtatc ggttctggtg aggaacgtat
    10081 tactgaagct gctcgaaaag caatttattc accacttctt gaaacaacga ttgatggagc
    10141 agaagacgtt attgtcaatg ttaccggcgg tatggatatg acacttactg aagcagagga
    10201 agcatcagag attgttagcc aagcagcagg taaaggtgtc aatatttggt taggcacttc
    10261 aattgacatg gatatgaaag acgaaatccg cgtaacagtt gttgcaacag gtgtgcgtaa
    10321 agataaaact aatcaagtat ctggttttac aacatcagct ccaactaatc aagcaccttc
    10381 agaacgtcaa agtacttcaa attcaaactt tgatcgtcgt ggcaactttg atatgacaga
    10441 gtctcgcgaa atgccaactc aacaaaatca gcctcataca caaaatcagc aacaaagttc
    10501 tgcttttggt aattgggacc ttcgtcgtga taatatttca cgtccgacag aaggtgaact
    10561 tgatagcaag ttatctatgt caacgttttc agaaaatgat gatatggatg atgaacttga
    10621 aacacctcca ttctttaaaa accgttaatt aaaatgatga atttacaaga aaataaaaca
    10681 gcgatttttg acaatgttag taaattggca ctaaaagcag gtcgcgctcc tgaatcagtt
    10741 catgtcgtag ctgtaacaaa atatgttaac tgtcaaacaa cagaagcgct tattagaaca
    10801 ggtgttaatc atatcggtga aaatcgtgtt gataaatttc ttgaaaaata tcaagcatta
    10861 aaagatgaaa agcttacatg gcatctcatt ggtagtttac aacgtcgaaa agttaaagat
    10921 gtcattaatt atgttgatta ttttcatgct ttagattctg ttaagttagc agctgagatt
    10981 caaaaacgtg ctcaaaaacc agttaaatgt tttctacaag ttaatatatc acgagaagac
    11041 agtaagcatg gttttactat tgagcaaata gatgatgcac tgaacttaat ttctcggtac
    11101 gataaaattg aacttatcgg catcatgact atggctccct taaaagcaac taaagaggaa
    11161 atatcatcaa tttttgaaga gactgagagt cttaggaaaa ggcttcaagc taggaacata
    11221 gaacgaatgc cgtttacaga attaagcatg ggcatgagta gagattatga tattgctatt
    11281 caaaatggat caacatttgt aaggatagga acttcattct ttaaataatg gaggggaata
    11341 tggcattaaa agatagattt gacaaaatta tttcttattt tgacactgac gatgtaagtg
    11401 aaaatgaagt acacgaagta caagagagaa cttcagtgca aagagattcc agagcagcta
    11461 cagcacagga agcttctcag cgtagtcata tgactaactc cgcagaggaa gagatgattg
    11521 gtagtcgtcc gagaacttct acctataatc ctaatcgtca ggagagacaa agggtacaga
    11581 gagacaatgc ttatcagcaa gctactccta ggattcaaaa taaagactca gttcgacaac
    11641 agagagaaca agtaacaatt gctctgaagt atccccgtaa gtatgaggat gcacaagaaa
    11701 ttgttgattt acttatcgtt aatgaatgtg tcttgattga ttttcaatat atgcttgatg
    11761 cgcaagcacg acgttgcttg gattatattg atggtgcaag tagagtatta tatggttcat
    11821 tacaaaaggt tgggagttca atgtttctat taacaccagc caatgttatg gtcgatattg
    11881 aggagatgaa tattccaaag actggtcaag agacgagttt tgattttgat atgaagagac
    11941 gataaatatg atattagtac tattaatatt attaaaaaca attagggtct attcttatat
    12001 cttactagcc tatgccttgt tatcttggtt tccgggagca tacgattcat ggattggtag
    12061 actagtggta cagattgttg atccgattat tcgcccactc agaagattta atctacaatt
    12121 tttaggtcta gattttacag taatagtagc attcttttta ctaaatatgt tgagtagatt
    12181 tttaatcttg ctttttacat aagatgacgt tagatgatat ttatcagcat tttcgaccag
    12241 aagagtatgc gtttatcaat aaaatagacc atttagctca atatgtcgaa aacacctatt
    12301 cgtttattac cactgaattt ctaaatccta gggaatttaa aatactcgaa agtgttctag
    12361 agaggcgagg tagtcattat tacacttctg gtcagtattt tcaaacagaa tatgttaaag
    12421 taataatagc accggaatac tatcagttag atatggttga ttttaacctt agcctaattg
    12481 aaataaagta taatgctaaa tttaatcacc ttacccatgc taaaattatg ggaactttac
    12541 tgaattactt aggtgtaaaa cgatcaattt taggggatat cctagttgaa gaaggctgtg
    12601 ctcaggtttt ggtcgatagt caaatgacaa atcaccttgt tcattcagtt acaaaaattg
    12661 gaactgctag tgtacaatta gctgaagttc ccttgtccaa acttctaact cccaaacaag
    12721 atattcaaaa actaactgtt attgcttcca gtttacgtct tgataaaatt ttagcaacga
    12781 ttttaaagat ttcacgaaca cagtcgacga aattgattga ggcagataaa gtcaaggtca
    12841 attatgcaat cgtcaatcga gtttctgaac aattagtaga gggggatttg attagtgtta
    12901 gaggatatgg tcgatttacg ttgaaccata acctagggtt aactaaaaat caaaaatata
    12961 agttagaagt agacaaaatg atacataact aaggagaaaa catgccactt acagcacttg
    13021 aaattaaaga taaaacattt tcatcaaaat ttcgcggtta tagcgaagaa gaagttaatg
    13081 aatttttaga gattgttgtt gacgattacg aagacttgat tagacgtaat cgtgagcaag
    13141 agcaatacat taaagattta gaagagaaaa tcgcttactt caacgaaatg aaggaatcgt
    13201 taagtcaatc agttatttta gctcaagaaa ctgctgaacg tgtgaaaatt tcagcacagg
    13261 atgaagcatc taacctaatg gggaaagcta catttgatgc tcaacattta attgatgagg
    13321 ctaaattaaa agcaaatcaa atccttcgtg atgcgacaga tgatgctaag cgtgtcgcta
    13381 tagaaacaga agatttgaaa cgtcaatcac gtgttttcca ccaacgctta ctttctgaat
    13441 tagaaggtca gttaaaatta gctaactcat ctgcttggga agaactgttg aaaccaacag
    13501 ctatttatct tcaaaattct gatgcttctt tcaaagaagt tgttgaaaaa gttcttgatg
    13561 aagatgatgc tttaccagtg gtggatgaca ctgagtcatt tgatgctact cgtcaattct
    13621 caccagatga aatggaagaa ttacagcgtc gtgttgaaga aagtaacaaa caacttgaag
    13681 agtcaggttt attagatact aataatttcc aaatggaaga accgatcaat ttaggtgaaa
    13741 ctcaaacttt taaattaaat attgaagatt aagaaaaaca aggttttaat attatattat
    13801 cagcgagtag gagatggtga gagccctaca atccctaata ttgaaattat cattttcaat
    13861 cattctacct gaactattat ttaagtaagg tagaaggctt aacccacctt acgggttgtt
    13921 gagggagtaa gaaatagcta ttttctgctc tgaattaagg tggtaccacg agctttcgtc
    13981 ctttatgtga tgaaagcttt tttatttagc aattttaaaa attgtgataa ctattactat
    14041 gagataagga gttagtatga aattaaaaga aaccttgaat ttaggacaaa cagcttttcc
    14101 aatgcgtgca gggcttccaa ataaggaacc tcaatggcaa gaagcatggg atcaagctga
    14161 catttataaa aaacgtcaag cattgaatga aggaaaacca gcctttcacc ttcatgacgg
    14221 acctccctat gctaatggga atattcatgt aggacatgca ctaaataaaa tttctaaaga
    14281 cattattgta cgttcaaaat caatgtcggg ttttcgagct ccttatgtgc ctggttggga
    14341 tactcatgga ctcccaattg agcaagtatt agctaaaaaa ggggttaaac gtaaagaaat
    14401 ggacttggct gaataccttg aaatgtgtcg tgattatgct ctcagccaag ttgacaaaca
    14461 acgtgatgat tttaaacgtc tgggcgtttc tgccgattgg gaaaatccct atattacatt
    14521 gacaccagat tatgaagcag atcaagtacg tgttttcggt gctatggcag ataaagggta
    14581 tatctatcgt ggtgctaaac cagtgtattg gtcatggtca tcagagtctg cccttgctga
    14641 ggctgaaatc gaatatcatg atattgattc gacatcactc tactatgcca ataaagttaa
    14701 agatggtaag ggaattcttg atacagatac ctatatcgtc gtttggacga caacaccatt
    14761 tactgtaaca gcttcacgcg gtttaacagt aggaccagat atggagtatg ttgtagttgc
    14821 accagtaggt agtgagcgta aataccttct tgcagaggtt cttgtagata gtctcgctgc
    14881 taagtttggc tgggaaaact ttgaaattgt gactcatcac actggtaaag aacttaatca
    14941 cattgttaca gaacatccat gggatacaga agtagaagag ttggttatcc ttggagacca
    15001 tgttacaaca gattctggta caggtattgt ccacacggct cctggttttg gtgaagacga
    15061 ctataacgtt ggtattgcta atggacttga tgttgtagtt accgtagata gtcgtggttt
    15121 gatgatggaa aatgctggtc ctgattttga aggtcaattc tatgacaaag tgacaccact
    15181 tgtaaaagaa aaattgggag atcttctttt agccagcgaa gttatcaacc actcatatcc
    15241 atttgactgg cgtacgaaaa agccaatcat ttggcgtgcg gttccacaat ggtttgcctc
    15301 tgtttcgaaa ttccgtcaag aaatcctcga tgaaattgaa aagacaaatt tccaacctga
    15361 gtggggtaaa aaacgtcttt ataacatgat ccgtgaccgt ggcgactggg taatctcacg
    15421 tcaacgtgct tggggtgttc cacttccaat tttttatgca gaagatggta cagctatcat
    15481 gacaaaagaa gtgactgatc acgttgcaga tttatttgct gaatacggct caatcgtttg
    15541 gtggcaacgt gatgctaagg accttctacc agcaggttat actcatccag gttcacctaa
    15601 tggtctcttt gagaaagaaa cagacatcat ggatgtttgg tttgattctg gttcatcatg
    15661 gaatggagta atgaatgctc gtgaaaacct ttcctatcca gcagatcttt atcttgaagg
    15721 ttctgatcaa taccgtggtt ggttcaactc atcactaatt acatcagttg ctgttaatgg
    15781 tcacgctcca tacaaagcag tcttatctca aggttttgta cttgatggta aaggtgagaa
    15841 aatgtctaaa tctctaggaa atacaattct tccgagtgat gttgaaaaac aatttggtgc
    15901 ggaaatcttg cgtctgtggg taacttctgt tgactcaagt aatgatgttc gtatttcaat
    15961 ggatatctta aaacaaacat cagaaactta tcgtaagatc agaaatacat tgcgtttctt
    16021 gattgcaaat acttccgatt ttaatcctaa acaagatgca gtagcctatg aaaatttagg
    16081 tgccgttgat cgttatatga ctattaaatt taatcaagtt gtggatacaa tcaacaaagc
    16141 ttatgctgct tatgatttta tggcaattta taaagctgtc gttaacttcg taacagttga
    16201 tctatcagca ttttaccttg attttgcaaa agatgtggtt tacattgaag cggctaacag
    16261 tccagaacga cgtcgtatgc aaactgtttt ctatgatatt ttagttaaac ttacaaaact
    16321 tttgactccg attctaccgc atactgctga agaaatttgg tcataccttg aacatgagga
    16381 ggaggagttt gttcaattag cagaaatgcc tgttgcacag acattctctg gtcaagaaga
    16441 aattcttgaa gagtggtcag cattcatgac gttacgaaca caagctcaaa aagctctcga
    16501 agaagcacgt aacgctaaag ttattggtaa atctctagaa gcacatttaa caatttatgc
    16561 tagtcaagaa gtgaaaacgt tattaacagc gttaaatagc gatattgcac tcttgatgat
    16621 tgtctcacaa ttaacaattg ctgatgaagc agacaaacca gctgactcag tatcatttga
    16681 aggtgttgca tttactgttg aacacgctga aggtgaagtc tgcgaacgct cacgccgtat
    16741 tgatccaacc acaaagatgc gttcgtatgg tgtagctgtt tgtgatgcta gtgcagcaat
    16801 tattgagcaa tattatcctg aagcagtagc tcaaggtttt gaagcttaat aaaaattatt
    16861 taacgctata ttttgacaaa tatcttgcca gttttaatgg aagaggttgg taagacattt
    16921 gtttagtagt catcaaaaaa gaagccttga ttaggcttct tttaattatg ctgttgttgg
    16981 atatgaaatt tcaattaact tggcagtatg gagtgatttc atctgctctt gaataataat
    17041 agattgatct attttacgct ttaagaaaaa ctcacgaata gcttctaatt cttcatcttt
    17101 gatagcacga tatttgtttg aaattaataa ttcataatgt ttaggtgctt ttgtaaaaac
    17161 gacatcggtg ttgccagctg aatagacttc cactaatttt gcatctgtat tttggagttg
    17221 atttcgaact agttcaggat gactactggt tgtattaatt agtctcataa ctattttccc
    17281 cctgattctt attaggttta ttataacact atttaggaag aaaggtatga attatttcct
    17341 agaatggtga tttttttccc aagcttggac accccatcta tggcttcctc gttttagctt
    17401 ttctttagct tcttggatag gaaaccaggc tagatggttg aaatcttcta gtggcgcttg
    17461 ctccttatga taggcagtaa cttcataaat ataggcggga ttatagtagt aagtatcacg
    17521 gtgtctagag taaaaatatt catcagcttg accatagtaa tggccaattg ttgcagaata
    17581 acctaattct tcaattaatt cacgggttaa agcttctagg tgattttcat tttcttcaat
    17641 ttccccacct ggaagaaacc aagcaccatt aggtgcctgt actaaaataa ttttatcatg
    17701 agttgggttt ggaataatag catagacacc aaatcgtgat ctatagtcta cattatctat
    17761 tttttcaccg aaagtaggat tagtcatagt tctctctcct gatt
//
LOCUS       DBJYSH010000037        17573 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.037, whole genome shotgun sequence.
ACCESSION   DBJYSH010000037 DBJYSH010000000
VERSION     DBJYSH010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 17573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17573
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.037"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            complement(185..2464)
                     /gene="pcrA"
                     /locus_tag="ACUBV3_000956"
     CDS             complement(185..2464)
                     /gene="pcrA"
                     /locus_tag="ACUBV3_000956"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HHU3847468.1"
                     /translation="MNPLIIGMNDKQAEAVQTTDGPLLIMAGAGSGKTRVLTHRIAYL
                     IDEKYVNPWNILAITFTNKAAREMRERAIALNPATQDTLIATFHSMCVRILRREADYI
                     GYNRNFTIVDPGEQRTLMKRIIKQLNLDTKKWNERSILGTISNAKNDLLDEIAYEKQA
                     GDMYTQVIAKCYKAYQEELRRSEAMDFDDLIMMTLRLFDQNKDVLAYYQQRYQYIHVD
                     EYQDTNHAQYQLVKLLASRFKNICVVGDADQSIYGWRGADMQNILDFEKDYPQAKVVL
                     LEENYRSTKKILQAANNVINHNKNRRPKKLWTQNDEGEQIVYHRANNEQEEAVFVAST
                     IDNIVREQGKNFKDFAVLYRTNAQSRTIEEALLKSNIPYTMVGGTKFYSRKEIRDVIA
                     YLNILANTSDNISFERIVNEPKRGVGPGTLEKIRSFAYEQNMSLLDSSSNVMISPLKG
                     KAAQAVWDLANLILTLRSKLDSLTVTEITENLLDKTGYLEALQVQNTLESQARIENIE
                     EFLSVTKNFDDNPEITVEGETGLDRLSRFLNDLALIADTDDIATETAEVTLMTLHAAK
                     GLEFPVVFLIGMEEGVFPLSRAIEDADELEEERRLAYVGITRAEQILFLTNANTRTLF
                     GKTSYNRPTRFIREIDDELIQHQGLARPVNSSFGVKYSKEQPTQFGQGMSLQQALQAR
                     KSNSQPQVTAQLQALNANNSHETSWEIGDVATHKKWGDGTVLEVSGSGKTQELKINFP
                     GIGLKKLLASVAPISKKEN"
     gene            complement(2570..2956)
                     /locus_tag="ACUBV3_000957"
     CDS             complement(2570..2956)
                     /locus_tag="ACUBV3_000957"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0047617 - fatty acyl-CoA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HHU3847469.1"
                     /translation="MKFNLEQVKVFENYEIENWEEGQVTLTTKVVDSSLNYYGNAHGG
                     YLFTLCDQVSGLVAISTGYEAVTLQSNINYLRAGRLDDLLTVIGTCVHNGRTTKVVDV
                     HIKNQKDEMVTTAIFTMFVTGERKEK"
     gene            complement(3089..4414)
                     /locus_tag="ACUBV3_000958"
     CDS             complement(3089..4414)
                     /locus_tag="ACUBV3_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020480284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015851 - nucleobase transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-xanthine permease family protein"
                     /protein_id="HHU3847470.1"
                     /translation="MTLNAKGESNQILVDKEVHSTNLLLDIDEKPELFQGLLLSFQHV
                     FAMFGATILVPLILGMPVSVALFASGCGTLIYQVATKFKVPVYLGSSFAYITAMALAM
                     KQMHGDISAAQTGILFVGLIYVVVATVIKFVGNSWVDKILPPIIIGPMIIVIGLGLAN
                     SAVTNAGFVAKGDWRKMLVAVVTFLIAAFINTKGKGFIKIIPFLFAIIGGYILSIILG
                     LVDLSPVEKAAWFELPKFYLPFKTGLFHSYKLYFGPEMLAILPISIVTIAENIGDHTV
                     LGQICGRNFLKKPGLNRLLIGDGLATAFSALIGGPAETTYGENTGVIGMTRIASVTVI
                     RNAAFIAIAFSFFGKFTALISTIPSAVLGGMAILLYGVIASNGLKVLIENRVNFAEVR
                     NLIIASSMLVLGLGGAVLDLGALTLSGTALSAIVGIILNLILPKESMKS"
     regulatory      4722..4854
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            5011..6357
                     /locus_tag="ACUBV3_000959"
     CDS             5011..6357
                     /locus_tag="ACUBV3_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HHU3847471.1"
                     /translation="MLTLFTHINSFVWGPPLLALLVGTGIYLSFRLGFVQLRQLSRAF
                     KLIFREDNGQGDISSYAALATALAATVGTGNIVGVATAIKSGGPGALFWMWVAAFFGM
                     ATKYAEGLLAIKYRTKDTNGEISGGPMYYIVNGMGQKWKPLAVFFSAAGILVALLGIG
                     TFTQVNAIASSLEHTFKISTRFTSLILAVIVLFIIFGGIKSISKVSEKIVPFMATSYI
                     LAALIIIAVNYNKIPHTFQLIFSSAFSGTAAIGGFSGAIVKEAIQKGIARGVFSNESG
                     LGSAPIAAAAAKTKEPVEQGLISMTGTFIDTIVICTLTGIAILVTGKWLEFDLQGAPL
                     TQASFNTVFGSLGSFALTFCLVLFAFTTILGWSYYGERCFEYLFGTKFINAYRIIFVI
                     MVGLGGFLQLDLIWVIADIVNGLMALPNLIALLVLSPIIVKETQKYFSETKNITKP"
     gene            6421..7656
                     /locus_tag="ACUBV3_000960"
     CDS             6421..7656
                     /locus_tag="ACUBV3_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HHU3847472.1"
                     /translation="MATSTENLQLAKRGPIISIIAYITLAVAKLAAGYWFDATSLVAD
                     GFNNLSDILGNVALLIGLHLASQPADSNHRFGHWKIEDLASLITSFIMFVVGIQVFIQ
                     TITKIINNTDTNIDPLGAIVGAISALVMLGVYFYNKQLSQRVKSSALVAASKDNLSDA
                     VTSIGTSIAIIAASLNFPIIDRLAAIIITYFILKTAYDIFIESAFSLSDGFDDYQLKQ
                     YEKAILTIPKISAVKSQRGRTYGSNIYLDIVLEMNPDLSVFESHAITERVEKLLSDKF
                     SVYDIDIHVEPASIPEDEIFDNVYQKLYKNEKIILAKIPGYETFISPDFYMINEKGNI
                     ITSDMLTNATNHSLASNFKYFNVKSISQKTKLVSYELEGKRHTSIWRRNEKWFLIYHQ
                     ITAKSSPYKTRRYQITSLD"
     gene            7815..8207
                     /locus_tag="ACUBV3_000961"
     CDS             7815..8207
                     /locus_tag="ACUBV3_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HHU3847473.1"
                     /translation="MKLYVQLMVILTFSFAGEVLSTIFNLPVPGSIIGLILLFLALKY
                     KIIRLRHIDAVGNFLLANMTILFLPPAVGLMEHFQDIKPYLFGIASIILGALFLNILT
                     IGLVSQWIKKRYEGDYPEIGGKNGNFNE"
     gene            8188..8883
                     /locus_tag="ACUBV3_000962"
     CDS             8188..8883
                     /locus_tag="ACUBV3_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HHU3847474.1"
                     /translation="MATLTNNPIFGIMLTVWAYYIGIRIFRKYPSPATTPLLLATILL
                     IAFLKLTHISYKDYYNGGSFLTMLITPSTVVLAIPLYRTFHLMKHHIKSISISIILAS
                     VINTVFTAIVAKFFGMKYFLAISLFPKSVTTAMAVGITSKAGGLATITLVVVVITGIL
                     TSVLGPIFLKLLRIEDPVAIGLALGGTGHAIGTGQALKYGQVQGAMAGLAIGITGICY
                     VIVSPLVAGLILK"
     gene            8952..9575
                     /locus_tag="ACUBV3_000963"
     CDS             8952..9575
                     /locus_tag="ACUBV3_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000612522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3862 domain-containing protein"
                     /protein_id="HHU3847475.1"
                     /translation="MIPLKKITVGTIACLSFLGLTACSSSNTQQTSTSKSNVSQHKNI
                     KADHEELRLKFNKVKLGVKANNFRGGTSLAELKQLFGGEPNEKFDTPAGNVTLKGYRW
                     NVDDISITIQLLNDSSIVRSISNFKFIRDANITTKDYNSLKNGMSYNKVKELLGEPDD
                     ISQAVSSDKEELQAAWISGIQSSDSDPGINLTFENDKLTNKQQHGLK"
     gene            complement(9836..9907)
                     /locus_tag="ACUBV3_000964"
     tRNA            complement(9836..9907)
                     /locus_tag="ACUBV3_000964"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9872..9874),aa:Arg,seq:ccg)
     gene            complement(9980..11182)
                     /gene="rpsA"
                     /locus_tag="ACUBV3_000965"
     CDS             complement(9980..11182)
                     /gene="rpsA"
                     /locus_tag="ACUBV3_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002997242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HHU3847476.1"
                     /translation="MNEFEDLLNSVSEVNPGDVVTAEVLTVDNGQANVVIDGTGVEGV
                     LTLRELTNDRDADINDFVKAGDTVEVLVLRQVVGKDTDTVTFLVSKKRLEARKAWDKL
                     VGREGEVVTVKGTRAVKGGLSVEFEGLRGFIPASMIDTRFVRNTEKFVGQEFDAKIKE
                     VDAAENRFILSRREVVEESAAAARKEVFSNIEVGSVVTGKVARLTSFGAFIDLGGVDG
                     LVHVTELSHERNVSPKSVVTVGEEVEVKVLSIDEEAGRVSLSLKATTPGPWDGVEQKL
                     AAGDVIEGKVKRLTDFGAFVEVLPGIDGLVHISQISHKRVENPKDVLSAGQEVTVKVL
                     EVNSDAERVSLSMKALEERPAQAEGEKEEKRQSRPRRPRRQEKRDYELPETQTGFSMA
                     DLFGDIEL"
     gene            complement(11308..11379)
                     /locus_tag="ACUBV3_000966"
     tRNA            complement(11308..11379)
                     /locus_tag="ACUBV3_000966"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11345..11347),aa:Gln,seq:ttg)
     gene            complement(11385..11465)
                     /locus_tag="ACUBV3_000967"
     tRNA            complement(11385..11465)
                     /locus_tag="ACUBV3_000967"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11429..11431),aa:Tyr,seq:gta)
     gene            complement(11526..11756)
                     /locus_tag="ACUBV3_000968"
     CDS             complement(11526..11756)
                     /locus_tag="ACUBV3_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001870505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HHU3847477.1"
                     /translation="MSKKDKKIEIQISDAQVTVNGTKVDGYQLVIGKKIVGQIAELDS
                     KFAVIKGEQVSGFYKNLDQAMGNIIEEYNLNR"
     gene            complement(11840..12862)
                     /locus_tag="ACUBV3_000969"
     CDS             complement(11840..12862)
                     /locus_tag="ACUBV3_000969"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012961903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HHU3847478.1"
                     /translation="MTVNLDWDNLGFAYRKLPFRYISHFKDGKWDDGKLTDDATLHIS
                     ESSPALHYGQQAFEGLKAYRTKDGSIQLFRPDQNAERLQRTADRLLMPHVPTDKFIAA
                     VKSVVRANEEFVPPYGTGATLYIRPLLIGVGDIIGVKPAEEYIFTVFAMPVGSYFKGG
                     LTPTNFIVSKEYDRAAPNGTGAAKVGGNYAASLLPGKYAHEKQFSDVIYLDPATHTKI
                     EEVGAANFFGITKDNQFITPLSPSILPSITKYSLLYLAKERFGMEAIEGDVFVDELDK
                     FTEAGACGTAAVISPIGGIQNGDDFHVFYSETEVGPVTRKLYDELVGIQFGDVEAPEG
                     WIYKVN"
     gene            complement(12976..15435)
                     /gene="parC"
                     /locus_tag="ACUBV3_000970"
     CDS             complement(12976..15435)
                     /gene="parC"
                     /locus_tag="ACUBV3_000970"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HHU3847479.1"
                     /translation="MSNIQNMSLEDIMGERFGRYSKYIIQERALPDIRDGLKPVQRRI
                     LYSMNKDGNTFEKGFRKSAKSVGNVMGNFHPHGDSSIYDAMVRMSQDWKNRETLIEMH
                     GNNGSMDGDPAAAMRYTEARLSEIAGYLLQDIDKNTVPFAWNFDDTEKEPTVLPAAFP
                     NLLVNGATGISAGYATDIPPHNLAEVIDAVVYMIDHPKAKLDKLMEFLPGPDFPTGAI
                     IQGKDEIRKAYETGKGRVAVRSRTAIETLKGGKKQIIVTEIPYEVNKSVLVKRIDDVR
                     VNNKVPGIAEVRDESDRDGLRIAIELKKEADETIVLNYLFKYTDLQVNYNFNMVAIDD
                     YTPKQVGLSRILTSYIAHRREIIIARSKFDKEKAEKRLHIVEGLIRVLSILDEVIALI
                     RASENKADAKENLKVSYEFSEAQAEAIVTLQLYRLTNTDIVTLREEEEELRQQITMLK
                     AIISDERTMYNVMKRELREVKKKFANTRRSELQELAETIEIDTASLIIEEDTYVSVTR
                     GGYVKRTSPRSFNASTVDELGKREDDELIFVSNAKTTQHLLMFTNLGNLAYRPVHELA
                     DIRWKDVGEHLSQNLVNFASNEEIIYAELVDDFTKETYFAVTSLGQIKRFERQEISPW
                     RTYKSKTAKYAKLKSVEDYVVTVAPIQLEDVILVTYNGYALRFSINDVPVVGSKAAGV
                     KAMNLKDRDHIVSAFIANTTSLYLLTHRGSLKRMAIDVIPTTSRANRGLQVLRELKSK
                     PHRVFKAGPVYLEDSSFEFDLFSSVSNHEGDTFVLEIMSKTGKVYDVDLSQWSFSERT
                     SNGSFVSDKISDEEVFSVKIK"
     gene            complement(15569..17518)
                     /gene="parE"
                     /locus_tag="ACUBV3_000971"
     CDS             complement(15569..17518)
                     /gene="parE"
                     /locus_tag="ACUBV3_000971"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HHU3847480.1"
                     /translation="MAKQDITVTNYGDDAIQVLEGLDAVRKRPGMYIGSTDGTGLHHL
                     VWEIVDNAVDEALSGFGNRIDVIINKDGSITVTDHGRGMPTGMHAMGKPTVEVIFTVL
                     HAGGKFGQGGYKTSGGLHGVGSSVVNALSSWLEVEIIRDGAIYRQRFENGGKPVTTLK
                     KIGTAPKSKSGTSVSFMPDQSVFSTIDFKFNTIAERLKESAFLLKNVTLTLTDNRSEE
                     AEYLEFHYENGVQDFVEYLNEDKETLTPIMFFEGEEQEFHIEVALQYNDGFSDNILSF
                     VNNVRTKDGGTHETGLKSAITKSMNDYARKTGLLKEKDKNLEGSDYREGLSAILSILV
                     PEEHLQFEGQTKDKLGSPLARPIVDGIVSEKLTYFLMENGDLASNLIRKAIKARDARE
                     AARKARDESRNGKKSKKDKGLLSGKLTPAQSKNAKKNELYLVEGDSAGGSAKQGRDRK
                     FQAILPLRGKVLNTAKAKMADIIKNEEINTMIHTIGAGVGPDFNLDDINYDKVIIMTD
                     ADTDGAHIQTLLLTFFYRYMRPLVEEGHVYIALPPLYKMSKGKGKKEIVEYAWTDIEL
                     EELRQKFGKGSLLQRYKGLGEMNADQLWETTMNPETRTLIRVTIEDLARAERRVNVLM
                     GDKVPPRRQWIEDNVKFTLEENTVF"
BASE COUNT         5530 a         3456 c         2814 g         5773 t
ORIGIN      
        1 aaagaatgca agagaaacgc ttctttcttt aaaatagtat ggtcactttt ggaaaacgct
       61 ttaatatcaa cctttttagt aagtaaaaac caaatgataa cttattttgt ctattcaaat
      121 gatgcaaaaa gactaagaat acaattctta gtctctgatt atttaagcat ataatagaag
      181 aaagttagtt ttccttttta ctaataggag cgacactcgc taataatttc tttaacccaa
      241 tacctggaaa atttattttt aactcttgag tctttccgct acctgaaact tcaagcaccg
      301 taccgtcccc ccatttttta tgagttgcaa catcaccaat ctcccatgat gtctcatggc
      361 tattgttcgc gttaagagcc tggagttggg cagttacttg aggttgtgaa ttacttttac
      421 gtgcctgaag cgcttgttga agactcattc cttgaccaaa ttgtgtaggc tgttcttttg
      481 aatatttcac accaaatgaa gaattaactg gtcgagccaa cccttgatgt tgtatcagct
      541 catcatcaat ctcacgtata aaacgggttg gtctattata actagtttta ccaaataaag
      601 tacgggtatt agcattggta aggaaaagta tttgttctgc tctcgtaatc ccgacataag
      661 ctaaacgacg ctcttcttct aattcatcag catcctctat tgcacgcgat aaaggaaata
      721 ctccttcttc cataccgatt aagaaaacaa ctggaaattc tagacccttg gcagcatgta
      781 atgtcattaa agtgacttca gccgtttcag tggcgatatc atcagtatct gctattaaag
      841 ctaaatcatt taaaaaacgc gatagacgat ctaaaccagt ttcaccctct actgtaatct
      901 ctgggttatc gtcaaaattt ttagtcactg ataagaattc ttcaatattt tcaatacgcg
      961 cttgactttc taatgtattt tgaacttgaa gtgcttctag atatcctgtc ttatccagta
     1021 ggttttctgt aatctccgtt acagttaaac tatctagctt actacgtaga gtcaaaatca
     1081 gattagctaa atcccaaaca gcttgagcag ccttcccttt taagggcgat atcatgacat
     1141 ttgaagagga atcaagaaga gacatgttct gttcataggc aaatgaccgt attttttcta
     1201 aagttcctgg ccccacccct cttttaggtt catttacaat ccgctcaaaa gaaatattat
     1261 cagaagtatt tgcaaggata ttgagataag caataacatc tcgaatttct ttacgactat
     1321 agaattttgt tccaccaacc attgtatatg gaatattgga ttttaaaagt gcttcctcaa
     1381 tagtacgaga ttgcgcattc gtacgataaa gaacggcaaa atctttgaaa ttttttcctt
     1441 gttctcgaac gatgttatca atagttgacg ctacaaaaac ggcttcttct tgctcgttgt
     1501 tagctctatg atatacaatt tgctcacctt catcattttg agtccataat tttttaggac
     1561 ggcgattttt attatgattg atcacattat tagcagcttg aagtattttc ttagttgatc
     1621 gataattttc ttctaataat acaactttgg cttgcggata gtccttttca aaatcaagaa
     1681 tattttgcat atcagctcca cgccatccat aaatggattg atcggcatca ccaacaacac
     1741 aaatattttt gaaacgcgaa gctaataact taactaattg gtattgagcg tggttagtat
     1801 cttgatactc gtctacatgg atatattgat acctctgttg gtaataggcc aaaacatctt
     1861 tattctgatc aaataatcga agtgtcatca taatcaagtc atcgaaatcc atggcctcac
     1921 ttctacgtaa ttcctcttga taagctttat agcatttagc aattacctgt gtatacatat
     1981 ctccggcttg cttctcatat gcaatttcat caagaaggtc atttttagca ttagagattg
     2041 tacctaaaat tgaacgttca ttccactttt ttgtgtccaa atttaattgc ttaatgattc
     2101 gcttcattaa agtacgctgc tcacctggat caacgattgt aaagttacga ttatatccaa
     2161 tataatcagc ttcacgacgt aaaatacgaa cacacatact atgaaaagtt gcaatcaaag
     2221 tatcttgggt agctggattc aaggcgatag cacgttcacg catttcacgc gctgctttat
     2281 tagtaaaagt aatcgctaaa atattccaag gattaacata tttttcatct attaaataag
     2341 caatacgatg agtcagaaca cgtgtttttc cagagccagc tcctgccata atcaaaagcg
     2401 gtccgtctgt cgtttgtacc gcttctgctt gtttatcatt cattccaatt attaaaggat
     2461 tcatttctac tcctaatttt gtcaaaattg cctctctagt atatcatttt ttcatctttt
     2521 tcccaataga aaaaggagtt aaacagacgc tgtctaactc ctaccttttt tacttctctt
     2581 ttctctctcc agttacaaac attgtaaaaa tagcagttgt aaccatttcg tctttctgat
     2641 ttttaatgtg aacatcaacg acttttgtag tacgaccatt atgaacacaa gtaccgatga
     2701 cagtaagcag atcatctaat cggcctgccc tcaaatagtt gatatttgat tgtagagtta
     2761 ccgcttcata tcctgttgaa atagcaacta aaccactgac ttgatcacac agtgtaaaca
     2821 aataaccacc atgagcatta ccataatagt tgagcgaaga gtcaacgacc ttcgttgtta
     2881 aggtaacttg accttcttcc cagttttcaa tttcgtaatt ctcaaatact tttacttgtt
     2941 ctaaattgaa tttcattcta ctataacctc actttttcta tgaccattat agcagaaaat
     3001 aaggaaacca taactgtaaa cgtttttatt ttaaagtttt taactgatct atcataacaa
     3061 aagagctcag atactctgag ctcttttgtt atgatttcat gctttctttt ggcaatatta
     3121 aatttaatat aatacccact atagcactta atgctgttcc tgataaagtt agagcaccta
     3181 gatcaaggac tgctccaccg aggcctaaaa ctaacatgga actggcaata atcagattac
     3241 gaacctcagc aaaattgaca cgattttcaa tcaacacttt taatccatta cttgcaataa
     3301 caccataaag taatatagcc attccaccaa gtactgcact aggaattgta gagatcaaag
     3361 ctgtaaattt accaaagaat gaaaaagcga ttgcaataaa agctgcatta cgaataaccg
     3421 ttacagaagc tatacgcgtc atgccaataa cacctgtatt ttcaccataa gtagtttcag
     3481 ctggccctcc tataagagca gaaaatgcgg ttgctaaacc atctccaatt aaaagacgat
     3541 ttaaacctgg tttttttaga aagttacgcc cacaaatctg acctaaaact gtatgatcac
     3601 cgatattttc agcaatcgtt acaattgaga ttggaagaat tgcaagcatt tctggtccaa
     3661 agtaaagttt ataactgtga aataaacctg tcttaaacgg taaatagaat tttggtaatt
     3721 caaaccaggc agctttttca actggagata aatctaccaa gccaagaatg attgataaaa
     3781 tataacctcc aataattgca aataaaaatg gaataatctt gataaaacct ttccctttag
     3841 tattgataaa agctgcaatc aagaatgtaa caacagcaac tagcatttta cgccaatcac
     3901 ctttagcgac aaaacctgca ttagttacag cagagtttgc caaacctaaa ccaataacaa
     3961 taatcatagg tccaatgatt attggtggca aaatcttatc aacccatgaa tttcctacga
     4021 acttaataac cgtcgccaca acgacataga ttagacctac aaaaagaata cctgtttgag
     4081 cagccgaaat atcgccatgc atttgtttca tggctaatgc catagctgta atataagcaa
     4141 atgatgagcc taggtaaaca ggaactttaa attttgtcgc gacttgataa atgagcgttc
     4201 cgcaaccaga agcaaataaa gccactgata caggcatccc taaaataagc ggaactaata
     4261 tcgtagctcc aaacatggca aaaacatgtt gaaaacttaa cagcaaacct tgaaataatt
     4321 ctggtttttc atcgatatct agtaataagt tagtagaatg tacttcctta tctactagaa
     4381 tttgattact ttcaccttta gcatttaaag tcatatctaa actagtcctc ttgggcatac
     4441 aaaaaagccc actttctata gcaatatagt cataggcttt atttttttcc tgatgttttt
     4501 ctcacctcac gggatgagtt taaaaaccta cgctgaaaga attatagcat aagacatttt
     4561 agaaaacaag acttttttaa aactatattg acaaaactat gctttttcat tatgatagtt
     4621 aacataataa aatataccga atgacgtcat tcaggagaag aaagttagac tttcgccgaa
     4681 ggaattacac tctcaggtgt cttaagacag gactgattga cagacggact tctggagaga
     4741 cctataagta gcaacatctt tgtattgaca ccaagatgtg ctctaggcgc cgaaggggca
     4801 agaagagtaa aacaactcct ccaatctctc aggcaaaagg acagaagcta aaagccaata
     4861 ttaataatga gtagtaagct tattaagttt actactacct ttatttgtgc gctttttagc
     4921 tagcatcttt cagaagttat ctcttttaga gataactttt ttcgtttcat tacagaatcc
     4981 ataggtatgt catgtatcaa aggagaacat atgctaacac tttttactca tatcaatagc
     5041 ttcgtttggg gtccaccttt acttgcttta ttagtcggaa caggtattta cctatcattt
     5101 cgcttaggtt ttgttcaatt gagacaactt tctagagctt tcaaattgat tttccgagaa
     5161 gataacggac aaggggatat ttcaagttat gctgctcttg caactgctct tgctgcaacg
     5221 gtagggacag gtaatatcgt tggtgtggct acggctatta aatctggagg accaggagct
     5281 ttgttttgga tgtgggtagc cgcctttttt ggaatggcaa caaaatacgc agaaggatta
     5341 ttagctatca aatatcgcac aaaagatact aatggagaaa tttctggagg accaatgtac
     5401 tacattgtca atggaatggg acaaaaatgg aaaccattag cagtcttttt ttctgctgca
     5461 ggtatcttag tagctctttt aggaataggg acattcactc aagtcaatgc aattgcctct
     5521 tctcttgagc atactttcaa aatatcaact agatttacca gcctcattct tgctgttata
     5581 gtattattta ttatatttgg aggtataaaa tctatttcaa aagtttcaga aaaaatagtg
     5641 ccttttatgg ctacaagcta tattttagca gctctgatta ttattgcagt taattacaat
     5701 aagattccac atactttcca actcatcttt agcagtgctt tttctgggac agcagccatt
     5761 ggtggttttt ctggagctat cgttaaagaa gcaattcaaa agggaattgc tagaggagtt
     5821 ttttcaaatg aatctggatt gggttctgct ccaatagccg cagcagcagc taaaacaaaa
     5881 gaacctgttg aacaaggcct tatttcaatg acaggaacct tcattgacac tattgttatt
     5941 tgtactttga cagggattgc aattttagtt acaggaaaat ggctagaatt tgatttacag
     6001 ggtgctccgt taacacaagc atcttttaat accgtatttg gctcactagg tagttttgcc
     6061 ctaacctttt gccttgtctt atttgctttt actactattt taggatggag ctattacgga
     6121 gagcgttgct ttgaatatct atttggcact aaatttatta acgcttatcg tattattttt
     6181 gtcattatgg ttggcttagg tggtttctta cagctcgatc ttatttgggt tatcgctgat
     6241 atcgttaatg gtttaatggc tttaccaaat ctaatagctc tcctagtttt gtcaccaatt
     6301 attgttaaag aaacacagaa atatttttca gagactaaaa atatcactaa gccttgaact
     6361 tactagtttt atagtctgct cctaagtgat ataatgtgta aatgaaagga aattaggaaa
     6421 atggcaactt ctactgaaaa cttacaactt gcaaaaagag ggcctattat tagtatcatt
     6481 gcctatataa cactggcagt cgctaaatta gctgcaggtt attggtttga tgctacttca
     6541 cttgtagcag atggatttaa taacttatct gatatcttag gaaatgtagc actcttaatt
     6601 ggtttacact tggctagtca accagccgac tcaaaccatc gttttggtca ctggaaaatt
     6661 gaagatttag caagcctgat tacctctttt atcatgtttg tcgtaggtat tcaagttttt
     6721 attcaaacta tcactaagat tattaataat actgatacta acattgatcc tctaggtgct
     6781 atagtaggtg ctatatctgc tcttgttatg cttggcgttt atttctataa taaacaatta
     6841 tctcaaagag taaagtctag cgctctggtt gcagcttcta aagataacct ttctgatgct
     6901 gtgacatcta tcggcacatc tattgctatc attgcggctt ctctaaattt tcctattatt
     6961 gatcgtttag ctgctatcat tattacttat tttattctta aaacagctta tgatattttt
     7021 atagaatctg ctttcagctt atcggatggt ttcgatgatt atcaattaaa acaatacgaa
     7081 aaagctatat taacaattcc taaaatatcc gctgtaaaat ctcaacgtgg acgtacttac
     7141 ggtagtaata tttaccttga tattgttctc gaaatgaacc ctgatctctc agtctttgaa
     7201 agtcatgcca ttaccgaaag agtggaaaaa ctactcagtg ataaattttc tgtatatgac
     7261 atagatattc atgtagaacc cgcttctatc ccagaagacg aaattttcga taatgtttac
     7321 caaaaattat acaaaaacga aaaaatcatc ctagcaaaaa ttcccggtta tgagaccttt
     7381 attagccctg acttttatat gattaatgaa aagggaaata ttatcacttc tgacatgctc
     7441 actaatgcta caaatcattc attggcaagc aactttaaat atttcaatgt aaaatcaatt
     7501 agtcaaaaaa caaaacttgt gtcttatgaa ttggaaggta aacgacatac tagtatttgg
     7561 cgccgtaatg aaaaatggtt cttaatctac catcaaataa ctgccaagtc aagtccgtat
     7621 aaaacgagac gatatcaaat cacttccctt gattaatata ctatttaatc agttactctt
     7681 gataaaaata gcttacaaaa ataaattaaa aagattgaga agaatctcgg tctttttaat
     7741 ttgtttttat gtaaacgttt catagccttg aaaattactt tcagagtagt agactataat
     7801 ggaaggagtt tttcatgaaa ttatacgttc aattaatggt tattttaacc ttttcattcg
     7861 ctggtgaggt tctatctact atattcaact tacctgtacc aggtagtatt attggattaa
     7921 tattactatt cctagcgtta aaatacaaaa tcatcaggct caggcatatt gatgccgtgg
     7981 ggaattttct attagcaaat atgaccattt tatttttacc accagcagtt ggactgatgg
     8041 agcactttca agacataaag ccctatcttt ttgggatagc tagcattatc ttaggagcgc
     8101 tatttctcaa tattctaact attggactgg tatcccaatg gatcaaaaag agatatgaag
     8161 gagattatcc agaaattgga ggtaaaaatg gcaactttaa cgaataaccc tatctttggt
     8221 attatgttaa ccgtatgggc gtattacatt ggcataagga tttttagaaa atatccttcc
     8281 cctgcaacaa ctccacttct tttggcgact attttattaa ttgctttttt aaaattaaca
     8341 catatctcct ataaagatta ctacaatggc ggttcatttc taacgatgtt aattacgcct
     8401 tcaacggttg tattggctat tcctctctat agaacctttc atttaatgaa gcatcacatt
     8461 aaaagtatta gcattagcat catcttagct tctgttatca acacagtttt taccgcaata
     8521 gttgctaagt tttttggtat gaaatatttc ttagcaatct cacttttccc aaagtccgta
     8581 actacagcta tggccgttgg aattacaagt aaagcaggtg gattagctac tatcactcta
     8641 gtcgtagttg ttattacagg catcttaaca agcgttttgg gtcctatttt cctaaaatta
     8701 ttaaggatag aagatcctgt tgctataggg ctcgcgctag gaggaactgg gcatgctata
     8761 ggaactggac aagccttaaa atatggccaa gttcaaggcg ccatggctgg actagcaatt
     8821 ggtatcacag gaatttgcta tgttattgtc agtcctttag ttgcaggctt aatattgaaa
     8881 tgaggataag gaaagtaaac cccaaataat tatgttataa tatttaaaaa tagaatttag
     8941 gataaataat tatgataccg ttaaaaaaaa taactgttgg aacgattgcc tgtctctcat
     9001 ttttgggttt aacagcatgc tcttcttcaa atactcagca aacctctact tcaaaatcaa
     9061 atgtatcaca acataaaaat atcaaagctg accacgaaga attacgtttg aaatttaata
     9121 aagtaaagtt aggggttaaa gcaaataact ttagaggagg cactagtctc gctgaattaa
     9181 aacaattatt tggtggcgaa ccaaatgaaa aatttgatac accagcggga aatgtcactc
     9241 ttaaaggcta tcgttggaat gtcgatgata tctccattac tatacaatta ttaaacgata
     9301 gtagcattgt tcgttcaatt agtaatttta agtttattcg tgatgccaac atcacaacaa
     9361 aagattataa tagtttaaaa aatggcatgt catacaataa agtaaaagaa cttctaggag
     9421 aacctgatga tatttctcaa gcagtttctt cagataaaga ggaactacag gctgcatgga
     9481 ttagtggcat tcagtcgtca gatagtgatc caggaattaa ccttacattt gaaaatgata
     9541 aactcactaa caaacaacag catggtttaa aataaaaagg cattaacgcc tttttattaa
     9601 taccttaatt acttattaga aacatttcat tacacaatgg tttaatctta gtaccttaac
     9661 ttagcccttt aatggtcata ctaactaatt atgatactaa cttacacaga tttcaaacta
     9721 attcttcctt atttgtcaac cctagtttta cttatcagaa atgaggagaa tcagtttgtg
     9781 aggcaaggtt ggagataaac taatattaat ataaagcttt atggagcatc tattatccat
     9841 cttaagggaa tcgaacccct atcccaagaa ccggaatctt gtgtgatatc cattacacta
     9901 aagatggtag tacatgttac attatagcat actttatcaa caaaaacaaa aagacttgct
     9961 aaagcaagtc ttttaattat tataattcaa tatcaccgaa taagtcagcc atagagaatc
    10021 cagtttgtgt ttctggaagc tcgtagtcgc gtttttcttg acgacgtgga cgacgtggac
    10081 gtgattgacg tttttcttct ttttcacctt ctgcttgcgc tggacgctct tcaagagctt
    10141 tcattgaaag tgatacacgt tctgcatctg aattaacttc aagaacttta acagtaactt
    10201 cttgtcctgc agataatacg tctttaggat tttcaacacg tttgtgtgaa atttgtgaaa
    10261 tatgtacaag tccatcaatg ccaggtaaca cttcaacaaa agcaccaaag tcagtcaagc
    10321 gttttacttt accttcaata acatcaccag cagcaagttt ttgttcaacg ccatcccatg
    10381 ggccaggtgt agttgcttta agtgaaagtg atacacgacc agcttcttca tcaattgaaa
    10441 gtactttaac ttcaacttct tcaccaacag ttacaactga tttaggtgaa acattgcgct
    10501 catgtgataa ttcagttacg tgtacaagtc catcaacacc accaaggtca atgaaagcac
    10561 caaagcttgt taaacgagca actttaccag ttacaactga acctacttca atgtttgaga
    10621 aaacttcttt acgtgcagca gctgctgatt cttcaacaac ttcacgacgt gaaaggatga
    10681 agcgattttc tgctgcatca acttctttga ttttagcatc aaattcttgt ccaacaaatt
    10741 tttcagtgtt acgtacaaaa cgagtatcaa tcattgaagc tgggatgaaa ccacgaagtc
    10801 cttcaaattc aactgaaagt ccgcctttaa cagcacgtgt acctttaaca gtaacaactt
    10861 caccttcacg accaacaagt ttgtcccatg ctttacgagc ttccaagcgt tttttagata
    10921 caaggaaagt tactgtatca gtatctttac ctacaacttg acgaagaaca agtacttcaa
    10981 ctgtgtcgcc agctttaaca aaatcattga tatcagcatc acgatcgtta gtcaattcac
    11041 gaagtgtcaa gacaccttca acaccagtac catcgataac aacgtttgct tgaccgttat
    11101 caactgttaa aacttccgca gtgacgacgt caccagggtt cacttcgcta acactgttta
    11161 gcaaatcttc aaattcattc attctaaaaa atcctccaac aaaagctagc cgagctagct
    11221 tttgataaca atatattttc tcaaggtaac ccgcaaggac aacaaatatc tctttgacgg
    11281 tgtcaaacaa aagtttgata cctttgactg gggtagctgg attcgaacca acgcatgagg
    11341 gagtcaaagt cccttgcctt accgcttggc tataccccaa ataatggaaa gagagggatt
    11401 cgaacccccg aacccgaagg agcggattta cagtccgccg cgtttagcct cttcgctatc
    11461 tttccgtaac aacactgact attctaccac agatgttgtt agcacgcaag ctttttaaat
    11521 aaatattagc gattaagatt atattcttca ataatatttc ccatagcttg atctaaattt
    11581 ttatagaatc cactcacttg ctcaccttta atcacagcaa atttagaatc tagctctgca
    11641 atttgaccta caattttttt tccaataacg agttggtatc catcaacctt agttccatta
    11701 actgttactt gagcgtctga aatttgaatt tcaattttct tatctttttt actcactatt
    11761 aactccaata tctcttttta ttgtttttta ttgtacacaa aatactaaaa gcttgcaaga
    11821 gcaagctttt agtatagact taatttactt tataaatcca cccttctggt gcttcaacat
    11881 caccaaactg gatccctact aactcatcat atagtttacg tgtcactgga ccaacttctg
    11941 tctcactgta gaagacatgg aagtcatctc cgttttgaat tcctccaatt ggtgaaataa
    12001 ctgcggctgt accacatgcg ccagcttctg tgaatttatc taattcgtca acaaagacat
    12061 ctccttcaat cgcttccata ccaaaccttt ctttagcaag gtaaagcaat gaatatttag
    12121 taattgaagg taaaattgaa gggctcaaag gggtgataaa ctgattatct ttagtaattc
    12181 caaagaaatt agctgctcca acctcttcaa ttttagtatg tgtcgctgga tcaagataga
    12241 taacatctga aaattgtttc tcatgagcgt atttcccagg aagaagacta gcagcatagt
    12301 taccaccaac ttttgcagct cctgtaccat tcggtgctgc tctatcatat tctttggaaa
    12361 caataaagtt agttggtgtt aaaccacctt taaaataact acctactggc attgcaaata
    12421 ccgtaaaaat atattcttca gcaggtttaa ctccaataat atctcccaca ccaattaata
    12481 agggacgaat gtataatgtt gcccctgtac catacggtgg aacaaactcc tcattagcgc
    12541 gtacaacaga tttaaccgct gcaataaatt tgtctgtcgg aacatgtggc attaaaagac
    12601 gatctgctgt tctttgaagt cgttctgcat tttgatctgg acgaaataac tgaatagagc
    12661 catctttagt tctataagct ttcaatcctt caaaagcttg ttgcccataa tggagagcgg
    12721 gtgaactttc tgaaatatgt agcgttgcat catctgtcaa cttgccatca tcccatttcc
    12781 catctttaaa atgcgaaatg tatcggaatg gtaattttct gtaagcaaaa cctaaattat
    12841 cccaatctaa atttactgtc ataaggtacc tctttctttg ggtttctaac tattgtacgc
    12901 tattatcatt attttttcaa tattatttgt aaaattcaga taaattacca aactagtaaa
    12961 aacacaaatg tgtttttact taattttaac tgagaatact tcttcgtcag aaatcttatc
    13021 agatacaaaa gagccattac ttgtacgttc agaaaaactc cattgactta aatcaacatc
    13081 gtaaactttt cctgttttag acataatctc aagtacgaaa gtatctcctt catgattcga
    13141 tactgaacta aataaatcaa attcaaaaga tgaatcctct aaatatacag gtcctgcttt
    13201 aaaaacgcga tgcggtttgg atttcaattc acgaagaact tgaagtcctc gattagcacg
    13261 actagttgtg gggataacat caatggccat tcgtttaaga cttcctctat gagttaataa
    13321 atataaactt gtggtgttag ctataaatgc agatacgatg tgatcacgat cttttaaatt
    13381 catcgctttt acacctgcgg ctttactacc gacgactggt acatcattta tactaaatct
    13441 caacgcataa ccattataag tgacaagtat aacatcctct aattggatag gggcaacagt
    13501 cacaacataa tcctctacac tttttaactt agcatattta gctgtttttg acttatatgt
    13561 ccgccatgga ctgatttctt gacgttcaaa acgctttatt tgccctaagc tagtgacagc
    13621 aaaataagtt tctttagtaa aatcatctac taattcagca taaataattt cttcatttga
    13681 agcaaagtta acaagatttt gtgataaatg ttcaccaacg tctttccatc gaatatcagc
    13741 taattcatga actggacgat aagcaagatt accaaggtta gtaaacatca ataaatgttg
    13801 ggtagtcttt gcattgctca caaatatcaa ttcgtcatct tctcgcttac caagttcatc
    13861 aacagttgat gcattgaaag agcgagggct ggtgcgtttt acatatccgc ctctagtaac
    13921 actgacgtat gtgtcctctt cgataattaa acttgcagta tcgatttcaa tagtttcagc
    13981 taattcttga agttcagaac gtctagtatt ggcaaatttc tttttgacct cacgtaactc
    14041 acgtttcatt acgttataca tcgtacgttc atcactaata attgccttaa gcattgtgat
    14101 ttgttgacga agttcttctt cttcttctct caacgtcaca atatcagtat ttgtgagacg
    14161 atataattgc aatgtgacga tagcctctgc ttgagcttct gaaaactcat agctgacctt
    14221 caaattctct ttcgcatcag ctttattctc agaagcacga attaatgcaa tcacttcatc
    14281 caaaatagaa agaaccctaa tcagtccctc aacaatatgg agacgttttt cggctttttc
    14341 tttatcaaat tttgaacgag caataataat ttctcttcgg tgagcgatat agctagttaa
    14401 tattcttgat agacccacct gcttaggggt gtagtcatca atagcaacca tattgaaatt
    14461 ataattaact tgtaggtctg tatatttaaa aagataattt aaaacaattg tttcatcggc
    14521 ttcttttttg agctcaatag cgatacgaag accatctcta tcagactcat cacgcacttc
    14581 tgctattcct ggaactttat tattaacacg aacatcatcg attcgtttta ctaaaactga
    14641 cttattaact tcataaggaa tttcagtaac aataatttgt ttcttaccac cttttaaggt
    14701 ttcaatagca gttcgcgagc gaaccgctac tctcccctta ccagtctcat atgccttacg
    14761 aatttcatct tttccttgaa tgatagcgcc agttggaaaa tctggaccag gtagaaattc
    14821 cattaattta tctaatttag ctttagggtg atcaatcatg tacacgacag catcaatgac
    14881 ttctgctaga ttatgtggtg gaatatctgt cgcatagccc gcagatattc ctgttgctcc
    14941 gttgactaaa agattgggaa aggcagctgg taaaacagtt ggttctttct ctgtatcatc
    15001 aaaattccac gcaaagggta cggtattctt atcaatatct tgaagaagat aaccagcaat
    15061 ttctgataga cgggcttccg tataacgcat agccgccgca ggatctccat ccatagaacc
    15121 attatttcca tgcatttcaa tcaatgtctc acgatttttc caatcttgag acatacgaac
    15181 catcgcgtca tagatagagg aatccccatg aggatggaaa ttccccataa cattaccgac
    15241 tgattttgca gatttgcgaa atcccttttc aaaagtatta ccatctttat tcattgaata
    15301 aaggattcga cgttgaacag gttttaagcc atcacgaata tccggcaacg cccgttcttg
    15361 aatgatatac ttagaatagc gcccaaaacg ctctcccata atgtcctcaa gggacatatt
    15421 ttgaatatta gacatatttt taattttctc ctagttggag tggcttagta ttatttttcc
    15481 acttatttga aatgaatgat tggctctctt gtctaacgca tacaatatta cttgtacact
    15541 ataaaacatg aataaccaag gctctttatt aaaatactgt attctcttct aaagtaaact
    15601 tgacattatc ttcaatccat tggcgtcttg gaggaacttt atcacccatc aagacattaa
    15661 ctcgacgttc tgctcttgct aaatcttcaa ttgttacacg aattaatgtc cttgtctcag
    15721 ggttcatagt ggtttcccac aattgatcgg cattcatttc cccaagtcct ttatatcgtt
    15781 gtaatagtga gcctttacca aatttttgtc gtaattcttc taactctata tccgtccaag
    15841 cgtactcaac aatttctttc ttaccttttc cttttgacat tttatacaaa ggtggtaaag
    15901 caatatagac atgtccctct tcaacaagag gtctcatata gcgataaaag aaagttaata
    15961 atagtgtctg aatatgagct ccgtcagtat cagcatcggt cataatgata actttatcat
    16021 aattaatatc gtcaagatta aaatcaggac caacacctgc accaatggta tggatcattg
    16081 tattaatttc ttcgtttttt ataatatcag ccatctttgc cttagctgta ttcaatactt
    16141 ttcctcgcaa cggtaaaata gcttgaaact ttctatcacg accttgctta gcagaaccac
    16201 ctgcagagtc tccttcgact aaatagagtt catttttctt agcatttttt gattgtgcag
    16261 gggttaattt tccagaaagt agacctttat cttttttcga ctttttccca ttcctacttt
    16321 catcacgtgc tttacgtgca gcttcacgtg catcacgtgc ttttattgcc ttacggatta
    16381 aattagatgc taaatctcca ttttccataa gaaagtaagt taacttttca gaaacaatac
    16441 catcaacaat tggtcgtgct aagggacttc ctagtttatc tttagtttga ccttcaaatt
    16501 gcaagtgctc ctcaggtaca agaatagata aaatggctga tagcccctca cgataatcag
    16561 aaccttcaag gtttttatct ttttctttaa gaagtcctgt tttacgtgca tagtcattca
    16621 tagactttgt aatagctgat ttcaagccag tttcatgtgt tccaccatct ttagtacgaa
    16681 cattattgac aaaagaaaga atattatctg aaaaaccatc attatactgc aaagcaactt
    16741 cgatatgaaa ttcttgttct tcaccttcga aaaacatgat tggtgtcaac gtttctttat
    16801 cttcatttaa atactctaca aaatcttgaa caccgttttc ataatgaaat tctagatact
    16861 ctgcttcttc tgaacgatta tctgttaagg ttaaagtaac attttttaag agaaaggcag
    16921 attcctttaa gcgctctgca attgtattaa acttaaaatc tatcgttgaa aatactgatt
    16981 gatctggcat aaagctgaca gatgttccag attttgattt tggtgccgtg ccgatttttt
    17041 tcagggttgt aacgggctta ccaccatttt caaaacgttg tctgtaaata gctccgtcgc
    17101 gaatgatttc aacttctaac caagaagaca aagcattaac tacagatgat ccaacaccat
    17161 ggagaccacc tgatgtttta taaccacctt gtccaaattt tccacctgca tgaagcaccg
    17221 tgaagatgac ttcaacagta ggttttccca tagcgtgcat tccagttggc ataccgcgtc
    17281 catggtctgt aacggttata ctcccatctt tgttaataat aacatcaatg cgatttccaa
    17341 aacctgatag agcttcatca actgcattat ctacaatttc ccacactaaa tgatgtaaac
    17401 cagttccatc tgtggaaccg atatacatcc ctggacgctt acgaacagca tctaatcctt
    17461 ctaagacttg aatagcatcg tcgccataat tcgtcactgt tatatcttgt ttagccaaag
    17521 tgacctccaa attttttcat cttttttata ttacaagttt tttgctgttt ttg
//
LOCUS       DBJYSH010000038        17080 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.038, whole genome shotgun sequence.
ACCESSION   DBJYSH010000038 DBJYSH010000000
VERSION     DBJYSH010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 17080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17080
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.038"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            complement(41..1105)
                     /locus_tag="ACUBV3_000972"
     CDS             complement(41..1105)
                     /locus_tag="ACUBV3_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HHU3847481.1"
                     /translation="MKQTKKEFILGLTIIITLTLLIMLIFLVMFNKSSNSKLKQTKDR
                     RILSTSTTRTSSSSHDQKIVEKSLEKKKMVMEQDMELMNAYRLYYDYAHLSLKEVVLA
                     YMSEYGIEKNSVAFSYKNLKTGQRDAMNDTQAMTAGSTYKLPLNMLVVDAVVKGKLSM
                     EERFDITETDYEYIGEHNNYVAAFSGAMSIDDMQKYSLVYSENTPAYALAERIGGMDS
                     AYSKFGRYGQSKGDIKNIQKNGNKVTTDYYIQVLDYLWKHRKKYNSLITYLEEAFPTD
                     YYRALIPSDVVVAQKPGYVREALNVGAIVKEEVPYIVAIYTAGLGGSTQEDSEINGVG
                     LYQLEQLCFVINQWHRVNMN"
     gene            complement(1206..2462)
                     /locus_tag="ACUBV3_000973"
     CDS             complement(1206..2462)
                     /locus_tag="ACUBV3_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HHU3847482.1"
                     /translation="MEATFTTTGALIGLALAILLIIKKVHPAYSLILGALVGGLIGGG
                     DLVTIVNTMVLGAQGMMSSILRILTSGILAGALIKTGSAEKIAESIIKKLGQQRAITA
                     LAIATMIICAVGVFIDIAVITVAPIALAIGKKANLSKSSILLAMIGGGKAGNIISPNP
                     NTIAASEAFKVDLTSLMVQNIIPAIAALVVTIILAKIVSKKNNDISYDSEEQVGSDLP
                     AFLPAISGPLVVICLLALRPLFGITIDPLIALPLGGLISILATGYLKETVPFVEYGLS
                     KVVGVSILLIGTGTLSGIIKASNLQFDMIHLLEFLNMPTFILAPLSGIFMGAATASTT
                     SGTTIASQTFAETLIKSGVPAVSGAAMIHAGATVLDSLPHGSFFHATGGAVNMAIKER
                     MKLISYEALIGLTSTIVAVVYYCFFG"
     gene            complement(2487..3629)
                     /locus_tag="ACUBV3_000974"
     CDS             complement(2487..3629)
                     /locus_tag="ACUBV3_000974"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000869902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HHU3847483.1"
                     /translation="MKVVVAIDSLKGSLSSLEAGNAIKESINEVIPGADVEVHPLADG
                     GEGTVEALTLGMGGTIEIIPVKGPLGEKVHASYGIIPQRQLAIIEMAAAAGITLIATE
                     ERNPLHTTTYGVGEMINDAISKGCRHFIIGIGGSATNDGGAGMLQALGYALLDKDNQE
                     ISLGAQGLADLKSISTDKVIEELKECDFKIACDVTNPLCGAQGCSSIFGPQKGADEDM
                     ITKMDTWLSNYATLATSVSEKADATIEGTGAAGGLGFAFLAFTNATLEPGIDIILSEI
                     NIEKAISEADLVVTGEGRLDGQTVMGKAPIGVAKLAKKYGKKVVAFSGSVTEDAILCN
                     QHGIDAFFPIVRRLISLDEAMSKEVAYKNMKETATQVFRLINLYNH"
     gene            3795..4889
                     /locus_tag="ACUBV3_000975"
     CDS             3795..4889
                     /locus_tag="ACUBV3_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family transcriptional regulator"
                     /protein_id="HHU3847484.1"
                     /translation="MKLRKQLAQQIVTSIKDVCQQDINFINTKGIIFASTNPKRVGEF
                     HEIGLKVAQTGQMIEVTDQESYFGTQAGINIPFYYNCELLATIGISGNPNQVGKYALL
                     AQKMTRLILKEHELDYLDFGRKNEASIVLHHLVEGRELDYYYLNQFLNQYHLSEKTDY
                     RLLTFEINSQKQKLLLSQSEMSLLNFFDKLDTAIYTFNYPNQYWLLLSDQMFDYYYPN
                     ILSKFECEKGLYKVGIGQKSSLSLLKRSYETSILALKALKGQQKVNLVDDLDLELLLT
                     SIDSNIKQYVLNKALVNLSENDKILLNSYFKHNLSLKECSQELFIHKNTVQYRLNKIY
                     ESTQLNPRNFKDATLLYLALKSEDIYLHKL"
     gene            complement(4958..6385)
                     /locus_tag="ACUBV3_000976"
     CDS             complement(4958..6385)
                     /locus_tag="ACUBV3_000976"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HHU3847485.1"
                     /translation="MTIFPKHFLWGGAVAANQVEGAFRTDGKGLSVQDVLPNGGLGDF
                     TAKPTPDNLKLEAIDFYHNYKNDIKLFAEMGFKVFRTSIAWSRIFPNGDDSAPNEAGL
                     QFYDNLFDELLKYNIEPLVTLSHYETPLHLAKTYNGWADRRLIAFFEKFAQTVMERYK
                     DKVKYWLTFNEVNSILHMPFTSGAIMADKSQLSPQELYQAIHHELVASARVTKLGRSI
                     NPNFKIGCMILAMPAYPMTSDPRDVLAARQFEQHNLLFSDIHVRGKYPTYIQSYFKNN
                     GIEIKFEEGDEEVLAQNTVDFLSFSYYMSVTQAYDFENYQSGQGNILGGLTNPYLTTS
                     EWVWQIDPIGLRLVLNQYYERYQIPLFIVENGLGAKDQLIETLDGDYTVEDDYRIDYM
                     NQHLVQVAKAIEDGVEIMGYTSWGCIDCVSMSTAQLSKRYGLIYVDRNDDGTGSLQRY
                     KKKSFGWYQKVIKTNGQSLFEHHNR"
     gene            complement(6402..8270)
                     /locus_tag="ACUBV3_000977"
     CDS             complement(6402..8270)
                     /locus_tag="ACUBV3_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HHU3847486.1"
                     /translation="MTKYQETAKAILAAVGGEKNIQHVTHCVTRLRLVLDNDEIVNDQ
                     VIKTIPNVIGVMRKNDQYQIILGNDVNNYYNAFLALGHFENTTREFSSQKKSSIFEKL
                     IETIAGVIKPLIPALLGGGMLKVIGILLPMLGITSSSSQTVAFINFFGDAAYYFMPIM
                     IAYSAASRFKVTPVLAATVGGILLHPAFVTMVAEGKPLSFFGAPVTLASYGSSVIPIL
                     IMVFLMQYIERWINKIVPSVMKSFLQPTLIILISGFLALVVVGPLGVIIGKGLSSAML
                     SIYHVAPWLALAILGAIMPLVVMTGMHWAFAPIFLAASVTTPDVLILPAMLASNLAQG
                     AASLAVAVKAKQKQTRQVAFAAGLSALLAGITEPALYGVTLKFKKPLYAAMISGGLVG
                     AYIGLVNIASYTFVVPSIIGLPQYINPQGGNNFSNAVIVAIATIILTFIITWFLGIDE
                     GENEKSSINAQEYTHIRSGLSKKETLYSPMVGNVLPLSKVPDETFSSKLLGEGLAITP
                     SVGEVYAPFDGEIISLFPTKHAIALKDDKGVEVLIHIGIDTVELNGEGFEQLVKVGDF
                     VKRGQLLLRMDIDFISSKGYSLISPVVVTNSIDQLEIIVKDEETMVTNEDDLLVIL"
     gene            complement(8263..9114)
                     /gene="licT"
                     /locus_tag="ACUBV3_000978"
     CDS             complement(8263..9114)
                     /gene="licT"
                     /locus_tag="ACUBV3_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HHU3847487.1"
                     /translation="MIIKRVLNHNAVISVTHQGLDVLLMGKGIAFKKRIGDRINSDAI
                     EKSFVLKNSDNMNRFTELFITVPEEVVACSERIINLGKIKLGKNLDEILYINLTDHIH
                     SAIERHEQRMVIQNPLRLEIQRYYPDEYSIGMKALELIKDELGICLTIDESAFIAMHF
                     VNAGLDNPFNEAHKITEIVSYIEQKVKIDFRTELDESSIDYYRFMTHTKLFAQRVLSG
                     MKYEDDDADLLLVVKKKYPREYKCVKEIGNNMAIQYQYQLNSSELLYLTVHVKRLVKN
                     LKEISDD"
     gene            complement(9452..10060)
                     /gene="sodA"
                     /locus_tag="ACUBV3_000979"
     CDS             complement(9452..10060)
                     /gene="sodA"
                     /locus_tag="ACUBV3_000979"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase SodA"
                     /protein_id="HHU3847488.1"
                     /translation="MAIILPDLPYAYDALEPHIDAETMTLHHDKHHATYVANANAALE
                     KHPEIGEDLEALLADVSQIPEDIRQAVINNGGGHLNHALFWELMSPEETQISQELSED
                     IDATFGSFEDFKAAFTAAATGRFGSGWAWLVVNAEGKLEVLSTANQDTPIMEGKKPIL
                     GLDVWEHAYYLNYRNVRPNYIKAFFEIINWNKVNELYQAAKA"
     gene            complement(10140..11177)
                     /gene="holA"
                     /locus_tag="ACUBV3_000980"
     CDS             complement(10140..11177)
                     /gene="holA"
                     /locus_tag="ACUBV3_000980"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HHU3847489.1"
                     /translation="MIAIEEIGRITPDNLGLVTVLAGEDLGQYAQMKEKLFQVIGFNK
                     DDLAYSYFDLSEEEYQNAELDLESLPFLSDYKVVIFDQFQDITTDKKTYLDEQAMKRL
                     EAYLQNPVDTTRLVICAPGKLDGKRRLVKLLKRDARVLEANTLKESDLKTYFQKYAHQ
                     EGLVFEAGVFDELLIKSNYDFSDTLTNIAFLKSYKTDGHISSNDVREAIPKSLQDNIF
                     DLTQDVLLGRIDLARDLVRDLRLQGEDEIKLIAIMLGQFRMFLQVKILASKGKSESQI
                     VSELSHYIGRKINPYQVKFAVRDSRNLPLAFLKEAIRILIETDYAIKRGTYDKDYLFD
                     LALLKIAHKKP"
     gene            complement(11231..11959)
                     /locus_tag="ACUBV3_000981"
     CDS             complement(11231..11959)
                     /locus_tag="ACUBV3_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000468969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HHU3847490.1"
                     /translation="MFHREDFSKALLKKALQPGMRVMDIGCGSGELTRLAADIVGKEG
                     DVVGIDINEQLLWQARHDNGYANVSYQNADLAALSSFETKSFDAIIGRRVLMYQKNPK
                     KVIESLTPLLKQNGFYVFQESDAMCLELAKPSFVNMNKIQSLIWQTVEKEGGHIHIGS
                     QLEQLMLEAGLIVDHFVSENVIQTSQTGTDLSWLLNIMKERIQKYSDLQGLEDVNLQK
                     ELEENNDHFIRDCQFGIISHRVDE"
     gene            12135..13127
                     /locus_tag="ACUBV3_000982"
     CDS             12135..13127
                     /locus_tag="ACUBV3_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HHU3847491.1"
                     /translation="MKNYRKLIVLLLLIFFAIFMGAYAYTHIVEKRSLTSNTIEKTLP
                     VVNQIKPQTIKEYQNYLTKVAKRNVLPVDIPQALNNEKVEITATDGMQTFTWNDKNNP
                     KQKVIFYVHGGSYIHQASELQYIFVNKLAKKLDAKVVFPIYPKAPTYNYSDAIPKIKK
                     LYQNTLASVTSHKQIILVGESAGGGLALGIADNLVTEHIKQPKEIILISPWLDIATNN
                     PKIEKVQKKDPLLKAWQLQQVAPYWANGKKNFKNPQVSPLYSSQFNKMAPISFFIGTH
                     DIFYPDNQLLHQKLAKENIKHHYIVGQKMNHVYPVLPIPEAETAFKQMLKIINH"
     gene            complement(13186..14130)
                     /locus_tag="ACUBV3_000983"
     CDS             complement(13186..14130)
                     /locus_tag="ACUBV3_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HHU3847492.1"
                     /translation="MTSISDIAKKAGVAKSTVSRVINHHPHVSDETRQKVMALITELD
                     YIPNQLARDLSRGKTQKIGVVIPHTRHPYFTQLINGLLDAAKTTDYQLVVMPSDYNQE
                     LELSYLKQLKMEAIDALIFTSRAISLDIIETYAKYGRIVVCEKLQEYNHLSSAYLDRY
                     SSFLEAFSDMKLRGLEHLVLLFSRNNESSATYQSALLAYQEVYGQLSSPYMVVGNVHD
                     FNDGLNLSYQLVKEVSIDGILATSDEVAAGLIKGYEESRKKCPYIIGQECLLVGQLLK
                     LPTIDHKSYYLGKLAFKQALAEKISQEVLFSEFLSQDN"
     gene            complement(14141..14950)
                     /locus_tag="ACUBV3_000984"
     CDS             complement(14141..14950)
                     /locus_tag="ACUBV3_000984"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HHU3847493.1"
                     /translation="MTIKRIFCDMDGTLLNSEGQVSKSNATLIREAAIPVTLVSARAP
                     MEMKDAVDALQLGGVQVAFNGGLIYRIGDNNQVLPIHTQIIKKSTVKQLLRGIRFHFP
                     QVSLSYYDLNNWYCDKIDEGIRYEHSLTQQCPTFIHNEDQFLEGHTNTFKIMMITFDE
                     ANMLELEKYLQSLELPEITIQRSGKAYLEITHLLAKKSKGIAYILQKEQLAREETAAF
                     GDGHNDLPMLEMVGYPIVMDNAFDDIKAIAYQLTKSNDEDGVGYGIQKFLK"
     gene            complement(15076..>17080)
                     /locus_tag="ACUBV3_000985"
     CDS             complement(15076..>17080)
                     /locus_tag="ACUBV3_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HHU3847494.1"
                     /translation="QRVPNHISQIKVRIDTISINGDVLSFQADASGNTYQAFYTLKNK
                     SEKDYFQNLDNNIMIIADIKLEEAEERRHFNGFDYRQYLKRHGIYRIAKVTKIKQIRL
                     FQHRSFFDLMFKWRRSAIVISQTFPNPMRHYMSGLLFGYLDKTFDDMSDLYSSLGIIH
                     LFALSGMQVGFFLGIFRYICLRIGLRLDHVWLLQIPFSLIYAGLTGFSISVVRALIQS
                     LLSHSGVKKDENFALCLLICLISLPHSLLTTGGVLSFAYAFILTMTSFDHFSSIKKVA
                     IESLTVSVGILPILTYYFSGFQPISIILTALLSFAFDIIFLPLLTVIFVLSPFVKLSC
                     INSLFEILEVLLKWTGQLFPRPLIFGKPSLFLLIVMIIILGLLYDYYHSKCFRYCSLL
                     IIFTLFFITKNPITNEVAILDVGQGDSILVRDWLGKTILIDTGGRVRFEQPEEWKQKV
                     NQSNAERTLIPYLKSRGISKIDNLVITHTDTDHMGDMEVISKHFKVARLITSSGSLTN
                     SQYVKRLSKIGVAVKSIEAGDKLSVMGSYLQVLYPWHKGDGKNNDSIVLYGHLLGKGF
                     LFTGDLEEEGEKQLLEAYPNLSVDILKAGHHGSKGSSSLSFLKKLSPSVVLVSAGKNN
                     RYQHPHQETLQRFQKIKSKIFRTDQSGTIRLTGWWKWHIQTVR"
BASE COUNT         5703 a         3322 c         2520 g         5535 t
ORIGIN      
        1 gttaatctaa gaggttttta ttattttgat agatttattt ctaattcata ttaacacgat
       61 gccattgatt aataacaaaa cataattgtt ctagctgata tagtcccact ccattaattt
      121 ctgaatcttc ctgagtagag ccacctaaac ctgcagtata aatagcaacg atataaggca
      181 cttcttcttt gacaattgct ccaacattca acgcctcacg gacataccct ggcttttgag
      241 caacaacaac atcagatggt atcaaagcac gataataatc cgttggaaaa gcttcttcta
      301 aataagtaat taaactatta tactttttac gatgtttcca gagatagtct agcacttgaa
      361 tatagtaatc tgttgtcact ttattaccgt ttttttgtat atttttgatg tctcctttag
      421 attggccata acgaccaaac tttgaataag cactatccat gccaccaata cgctcagcta
      481 aagcataggc gggtgtattt tctgaataaa ctaaagagta tttttgcata tcatcaatag
      541 acattgcccc cgagaaagca gcaacataat tattatgttc accaatatat tcatagtcag
      601 tctcggtaat atcaaagcgt tcttccatgg acaacttgcc cttgaccact gcatctacta
      661 ccaacatgtt aagaggtaac ttataggtgc tccctgctgt catagcctga gtatcattca
      721 tagcgtccct ttgtcctgtt tttaaatttt tataagaaaa agcaacacta tttttttcaa
      781 tgccatattc agacatatag gccagaacaa cctctttcaa ggacaaatgg gcataatcat
      841 aatagagcct ataggcattc atcaattcca tatcttgttc catgaccatt ttcttttttt
      901 caagagactt ttctacaatt ttttgatcat gtgagctact ggatgtcctt gtggttgaag
      961 tagataatat tcttctatct tttgtctgct tcaacttaga gttactggac ttattaaaca
     1021 ttactaaaaa aatcagcatg attaataaag taagggtaat aataatggtt aatcctaaaa
     1081 taaattcttt ctttgtttgt ttcacaacac ctctcctact aatttattat agtaagaaaa
     1141 ttagaaaaaa tcaatgagaa ttctattcaa acagtgataa taaacatcac atattcatcc
     1201 aaattttacc caaagaaaca atagtaaacg acagcaacta ttgtgctagt taatccaatc
     1261 aaagcttcat agctaattag tttcatacgt tcttttatag ccatattgac agctccgcct
     1321 gtcgcatgga agaaggatcc atgaggtagc gaatccagaa cagtcgcacc tgcgtgaatc
     1381 atagctgcac ccgaaaccgc cggtacccct gatttaatca aggtttcagc aaacgtttgt
     1441 gaagcaatag ttgttccaga agtcgttgat gctgttgctg ctcccataaa aatacctgag
     1501 agtggggcaa ggataaaggt cggcatattt aaaaattcaa ggagatgaat catatcaaat
     1561 tgtagattag atgctttaat aatacctgaa agtgtacctg ttccaatcaa aagaattgaa
     1621 acgccaacaa cttttgataa accgtattca acaaacggta ccgtttcttt taggtaacct
     1681 gttgctaaaa tactaattag accacccaac ggcagtgcaa tcaagggatc aatagtgatt
     1741 ccaaataaag gacgaagagc taataagcag ataaccacaa gtggaccaga aatagccggt
     1801 aagaatgctg gcagatcaga gcccacttgc tcctcactat cataagaaat atcgttattt
     1861 ttcttggaaa caatctttgc taatataatc gtaactacta aagctgcaat tgcaggaata
     1921 atattttgta ccattaagct tgttaaatca accttaaatg cttctgaagc agcaattgta
     1981 ttaggattgg gtgaaataat attcccagcc ttaccaccac caatcattgc taacagtatg
     2041 ctagatttag aaagattagc ttttttacct attgctaatg caatcggagc aactgtaata
     2101 actgcaatat caataaagac acctactgca caaataatca ttgttgcgat ggctaaagct
     2161 gtgatggctc gctgttgtcc taatttttta atgatacttt ctgcaatttt ttcagctgat
     2221 ccggttttaa ttaaagcacc cgctaaaatc cctgacgtta aaatgcgtaa tatggatgac
     2281 atcatccctt gcgcacctaa taccatagta ttaacaattg taactaaatc accgccacca
     2341 atcaaaccac caactaatgc tccaagaatg agactatagg caggatgaac ttttttgata
     2401 atcagtaaaa tagcaagcgc taatcctatt aaagcccctg ttgttgtaaa tgtagcttcc
     2461 ataatttatc agaattcctt tcaatattaa tgattataca aattgattaa tctaaagact
     2521 tgagtagcag tttctttcat atttttataa gctacctctt tagacatagc ctcatctaag
     2581 ctgataaggc gtcgtacgat tggaaaaaaa gcatcaatac catgttgatt acaaagtata
     2641 gcatcttcgg tgacagatcc agagaatgcc actactttct taccatattt tttagctaat
     2701 tttgctacgc caattggagc ttttcccatc accgtttgcc catccaaacg accttcacca
     2761 gtaactacta aatcagcttc agaaatcgct ttttctatgt taatttctga tagaataata
     2821 tcaataccag gttctaaggt ggcatttgta aaggctaaaa aggcaaatcc aagcccaccc
     2881 gctgcacctg ttccttcaat agttgcatct gctttttcac taactgaagt agctaaagta
     2941 gcataattac ttaaccaagt atccattttt gtaatcatat cttcatcagc acctttttgc
     3001 ggtccaaaaa tcgaactaca accttgagca ccacataaag gatttgttac atcacatgca
     3061 attttaaaat cgcattcttt taattcctca ataaccttat cagtagagat agattttaaa
     3121 tctgccaatc cttgtgctcc caaactgatt tcttgattat ctttatctaa taatgcatat
     3181 cccaaagctt gtagcatacc tgccccacca tcattagtag cactacctcc aataccaata
     3241 ataaagtgcc tacagccttt acttatagca tcatttatca tttctccaac tccataagtg
     3301 gttgtgtgga gaggatttct ctcttcagta gcaattaggg taataccagc agctgcagcc
     3361 atttcaataa tagctaactg acgctgtgga ataataccat aactcgcatg tactttttct
     3421 ccaagaggcc ccttaacagg gattatttcg atcgtccccc ccatacctag cgtcaaagct
     3481 tcaacagttc cttctccccc gtctgctaat ggatgtactt ccacatccgc ccctgggatt
     3541 acttcattaa ttgactcctt tatagcattg ccagcttcta aagaagaaag actccctttc
     3601 aaagaatcaa tagctactac taccttcatt tgaaaacctc cttgaaaata ttttgtgaat
     3661 ctttgttatt ttttcactat tttatatcac tcaattatag taacaaaatc aaataacaaa
     3721 aaatatagac tatagaacaa atgttagcgt ttttatgata tgatatttgt tatatataac
     3781 aaaaggtaag ctgaatgaaa ttaagaaaac aactcgcaca acaaatcgtt acctctatca
     3841 aagatgtttg tcaacaagat attaatttta ttaataccaa aggaatcatt ttcgctagta
     3901 ccaatccaaa acgtgttgga gaattccatg aaattggttt aaaagtagca cagacaggac
     3961 aaatgattga agtaacagat caagaatctt attttgggac acaagcaggg atcaatattc
     4021 ctttctatta taattgtgaa ttattagcta ctattgggat tagtggaaac cccaaccaag
     4081 taggaaaata tgctttactt gctcaaaaaa tgactcgact catcttaaaa gagcatgaat
     4141 tagattatct ggatttcggg cgaaaaaacg aagcttctat tgtcttacac catcttgttg
     4201 aaggcagaga gttagactac tattacctca atcaattttt aaaccaatac cacctatcag
     4261 aaaaaactga ctatcgtcta ctcacttttg aaatcaatag tcaaaaacaa aaattactct
     4321 taagtcaatc tgaaatgtca ctcttgaact tttttgataa acttgacact gctatttata
     4381 cctttaatta tcccaatcaa tactggcttt tactttctga ccagatgttt gactattatt
     4441 atccaaatat cctatcaaag tttgaatgcg aaaagggtct ctataaagtt ggtatcggac
     4501 aaaaatcatc tttatcactt ttaaaaagat catatgaaac gtctatctta gccctgaaag
     4561 ctttaaaagg tcaacaaaag gtcaacctcg tcgacgacct tgatcttgaa ctgttattaa
     4621 caagtattga ttctaatatt aaacaatatg tgctaaataa agcgcttgta aacttatctg
     4681 aaaatgataa gatcttgctt aactcatatt ttaagcataa tctatcatta aaagaatgta
     4741 gtcaggaact ttttatccat aaaaatactg ttcaatatag gttaaataag atttatgaat
     4801 caacgcaact caatccaaga aactttaagg atgcaacttt attgtatcta gcacttaaat
     4861 cagaagatat ttaccttcat aaattataat aaaaaggagt aacctatgga tactcctttt
     4921 tatattaaat tgaaacagag actagttgtt taacatgtta tctgttatga tgttcaaaca
     4981 atgattggcc atttgtctta atcacttttt ggtaccaacc aaatgacttc ttcttgtaac
     5041 gttgtagact ccctgttcca tcatcatttc gatcaacata tattagacca taacgtttac
     5101 ttagttgagc tgtagacatg gatacacaat caatgcatcc ccaagatgta tatcccatta
     5161 tttcaacacc atcttcaata gctttagcaa cttgaactaa atgttgattc atataatcaa
     5221 tacgataatc atcttcgaca gtataatcac cgtctagtgt ttctatcaat tgatcttttg
     5281 ctcccaatcc attttctaca ataaacaacg gaatctgata acgttcataa tactgattta
     5341 agactaggcg tagccctatg ggatcgattt gccataccca ttctgaagtt gttaaataag
     5401 gattagtcag gcctccaagg atatttccct gaccactttg ataattttca aagtcataag
     5461 cttgtgtcac gctcatatag taactaaacg ataggaaatc tactgtattt tgtgccaata
     5521 cttcctcatc tccttcttca aatttaattt caataccatt atttttgaaa taggactgaa
     5581 tataagtggg atatttacct ctgacatgta tatctgaaaa tagcagatta tgttgttcaa
     5641 attgtcttgc agccagaaca tctcttggat cagatgtcat aggataagct ggcatagcca
     5701 gaatcataca gccaatttta aaattagggt tgatagaacg ccctaatttt gtcactctag
     5761 ccgatgctac taattcatga tgaatagctt gataaagctc ctgaggactt agttgtgatt
     5821 tgtcggccat aatagcacca ctggtgaaag gcatatgtaa aatagaattc acttcattaa
     5881 aagttaacca atattttacc ttatctttat aacgctccat gactgtttga gcaaatttct
     5941 caaaaaatgc tatcaaacgt ctatcagccc aaccattata ggtctttgca agatgaagcg
     6001 gtgtttcata atgagataag gtaaccaaag gctcgatatt atatttaagc aactcgtcaa
     6061 ataaattatc ataaaattgt aagccagctt cgtttggagc actatcatca ccatttggaa
     6121 aaattcgaga ccaggcaata gaggttctaa aaactttaaa gcccatctct gcaaacagtt
     6181 ttatatcatt tttatagtta tgataaaaat caattgcttc tagcttcaaa ttgtctggag
     6241 taggtttggc ggtaaaatcg cctagaccac catttggaag tacatcttgt acagataaac
     6301 ctttgccatc tgttctaaat gcaccctcta cctgattggc agcaactgca cctccccata
     6361 gaaaatgttt aggaaaaatc gtcatattat catccttcct tttaaagaat tacaagtaaa
     6421 tcatcttcat ttgtaaccat tgtctcttcg tctttaacga taatttcaag ttggtcaatt
     6481 gaatttgtaa ctacaactgg actaataaga gaatacccct ttgaactaat aaaatcaata
     6541 tccattcgca aaagtaactg tcctcgttta acaaaatcgc caactttaac caattgttca
     6601 aaaccttctc catttagttc tacagtatct attccaatat ggattaacac ttcaactccc
     6661 ttatcatcct ttaaggcaat ggcatgtttt gtaggaaata aacttataat ttcaccatca
     6721 aatggtgcat aaacttcacc cacactagga gtgattgcta aaccttcacc tagtaattta
     6781 gatgaaaatg tttcatcggg tactttagat aaaggtaata cattgcctac cataggtgaa
     6841 tacaatgtct ctttttttga taaaccactt ctaatatgtg tgtactcttg agcattaata
     6901 ctactttttt cgttttctcc ttcatcaatt cccaagaacc atgtaataat gaaagtcaaa
     6961 ataatagtag ctattgctac aataacagca ttactgaaat tattgccccc ctgtggattg
     7021 atgtattgtg gcaaaccaat aatagatggc actacaaatg tataggaagc aatattaact
     7081 aatccaatat aagctcctac taaaccacct gatatcattg cagcgtataa aggcttcttg
     7141 aatttcagtg tgacaccata aagtgcaggt tctgtgatac cagctagtag tgctgataaa
     7201 cctgctgcaa aggccacctg acgtgtctgt ttttgtttag ccttaactgc aacagcaagg
     7261 gaagctgcac cttgagctaa attagaagct aacattgctg gtaaaatcaa aacatctggt
     7321 gtcgtaacag aagcagctaa aaagattgga gcaaatgccc agtgcattcc tgtcataaca
     7381 accaatggca taatagcacc aagaatagct aaagctaacc atggtgccac atggtaaatc
     7441 gaaagcattg cactagacaa tcctttacca ataattactc ccagtggccc cacgaccact
     7501 aaggctaaaa agccagaaat gagaataatc aaagtcggtt gtaaaaagct cttcattaca
     7561 ctaggaacaa ttttattaat ccatctctca atgtattgca taagaaaaac cataattaag
     7621 attggaataa cagaagaacc ataactagcc agtgtaaccg gagctccaaa gaaagataat
     7681 ggttttcctt cagctaccat tgtcacaaaa gcagggtgta agagaatccc tcctactgta
     7741 gcagctaata caggtgtaac tttaaatcgt gacgcagctg aataagcaat cattattggc
     7801 ataaaataat aagcggcatc accaaaaaaa ttgataaaag ctactgtttg agaactagag
     7861 cttgttatac cgagcatagg gagcaaaata cctataacct tgagcatacc tcctcctaga
     7921 agagcaggta taagcggctt aatgactcct gcaatcgttt caattaattt ctcaaatatt
     7981 gaactctttt tttgtgatga aaattctctg gtagtatttt caaaatgacc taaggccaaa
     8041 aaagcattat aataattatt gacatcattt cccaaaataa tttggtattg atcattttta
     8101 cgcataacac cgataacatt cggaatagtt ttaataacct gatcgttgac aatttcatca
     8161 ttgtctaaaa ctaatcgtaa tcgtgtcaca cagtgggtta cgtgttgaat atttttttct
     8221 ccccctactg ctgctaaaat agctttagca gtctcttggt atttagtcat ctgatatctc
     8281 ctttaaattt ttaactaatc gtttaacatg caccgtcaga tataggagtt cacttgaatt
     8341 aagttgatat tggtattgga tagccatatt gttgccgatc tctttaacac atttatattc
     8401 tctcggatat tttttcttaa ctaccaaaag taaatcagca tcatcatctt cgtatttcat
     8461 tcctgataaa acgcgttgag caaataattt ggtatgtgtc atgaaacgat aataatcaat
     8521 tgaactttca tctagttctg ttcgaaaatc tatttttact ttctgttcaa tgtaagatac
     8581 aatctcagtg attttatgcg cttcattaaa cggattatcc aatccagcat ttacaaagtg
     8641 catagcaata aaagctgact catcaattgt taaacaaata cccaattcat cctttataag
     8701 ttccagagcc ttcatcccaa tggagtattc atcaggatag taacgctgga tttccaacct
     8761 tagtggattt tggataacca ttctttgttc atgtctttca atagccgaat gaatatgatc
     8821 tgtcaaattg atataaagaa tttcatctaa gttcttaccc aatttgattt tacctaggtt
     8881 gataattcgt tctgaacatg caactacttc ttctggaaca gtaataaata actccgtaaa
     8941 acggttcatg ttatcactat tttttaaaac aaatgacttc tcaatagcat cgctattaat
     9001 cctatcacct attcttttct taaatgcaat tcctttcccc atcagtaaca catctaatcc
     9061 ttgatgtgtt actgatatga ctgcattatg atttaatacg cgttttataa tcattaaagt
     9121 cctagtcctt cactcgcaca ataaaaaatg acccatccaa gacacaacaa aaagtcgtcg
     9181 ttgaataggt ccctgctcac aaaatgtgat aacatccttt attatgcaat tttatctgtt
     9241 atcagcttgc ttaccttaaa taataacacc tgaaaacgtc ttaattttat catttcttac
     9301 taatcttgtc aagcgctttc aataaactta ttcgcttaat tgaagataat aggactgttc
     9361 tagttctatc tgagtttgga tttctagact tttaaaaaca ttatcaactt ttagaataat
     9421 aaaaaaacga gcctatcgac tcgttttttt attaagcttt agcagcttgg taaagttcat
     9481 ttactttatt ccagttaata atttcaaaga aagctttgat gtagttagga cgaacattac
     9541 gatagtttaa gtaataagca tgctcccata catcaagtcc taaaataggt ttcttacctt
     9601 ccataattgg agtatcttga ttggcagttg aaagcacttc aagtttgcct tcagcattaa
     9661 caacaagcca agcccaacct gaaccaaaac gtcctgttgc tgctgctgtg aaagcagctt
     9721 taaagtcttc aaatgaacca aaagttgcat caatgtcttc agataactct tgtgaaattt
     9781 gagtttcttc tggtgacatc aattcccaga aaagagcgtg gttaagatgt ccaccaccgt
     9841 tattgatgac tgcctgacga atatcttctg gaatttgaga aacatcagct aagagcgcct
     9901 ctaagtcttc tccaatttca ggatgtttct caagagcagc atttgcatta gcaacataag
     9961 ttgcatggtg cttatcatga tgtagtgtca ttgtctcagc atcaatatgt ggctcaagcg
    10021 catcatatgc atagggtaaa tctggtaaaa taattgccat gtcgaaatcc tcttttcatc
    10081 tattgttacc cttattctat aggaatttgg aacagctttc aactcttttg tttctttaat
    10141 tagggctttt tatgagcaat ttttaataag gctaaatcaa agagataatc cttatcataa
    10201 gtccctcttt taatagcata gtcagtctcg attaaaatcc taatcgcttc ttttaaaaaa
    10261 gcaaggggaa ggtttctaga atctctgaca gcaaatttta cttgataagg gttgatttta
    10321 cgtcctatgt aatgggatag ttcagaaaca atttgactct ctgactttcc tttactagcc
    10381 aatattttaa cttgcaaaaa catccgaaac tgtcctaaca tgattgcaat caatttgatt
    10441 tcgtcttcgc cttgtagacg taaatctctt actaaatcac gcgctaaatc tatcctacca
    10501 agtaagacat cttgagtcaa atcaaatata ttatcttgca ggcttttagg gatagcctct
    10561 ctaacatcat tagacgaaat atgaccgtct gttttatatg actttaagaa agcgatatta
    10621 gttaaagtat cactaaaatc atagttagac ttaatcaata actcatcaaa aacacctgct
    10681 tcaaaaacta atccttcttg gtgagcatat ttttgaaaat acgtctttaa atctgattct
    10741 tttaaagtat tagcctctaa aacacgagca tcacgtttca ataactttac aagtcgacgt
    10801 tttccatcaa gttttcctgg agcacaaata acaagtctcg tagtatctac aggattctgc
    10861 aagtaggctt ccaagcgctt cattgcttgt tcatctaaat aagttttttt atcagttgta
    10921 atatcttgaa actgatcaaa aataacaacc ttgtagtctg ataaaaaagg taaactttct
    10981 aagtctaatt ccgcattttg atattcttct tcacttaaat caaaataaga ataggctaaa
    11041 tcgtctttat tgaatccaat tacctgaaat agcttctctt tcatctgagc atattgccct
    11101 aaatcttctc ctgctaaaac agttactaac cctaaattat ctggggtaat tctcccaatt
    11161 tcttcaatcg caatcatatt ccttattata ccataaggaa acttttcaag gtgtcaaata
    11221 atacttaaaa tcattcatca actctatgag aaataattcc aaattgacaa tctcggataa
    11281 aatggtcatt attctcttct aattcttttt gtaaattgac atcttctaac ccctgaaggt
    11341 cactatattt ttgtattctt tctttcatga tatttagtag ccaagataga tctgtaccag
    11401 tttgtgacgt ttgaatgaca ttttctgata caaaatggtc aacgattaag cctgcttcta
    11461 acattaattg ctcaagttga cttccaatat gaatgtgtcc tccttccttc tcaacagttt
    11521 gccagattag tgattgtatt ttattcatat ttacaaagct aggcttagct agttctaaac
    11581 acattgcatc actttcttga aaaacataaa aaccattttg ctttaacaaa ggtgttaaac
    11641 tttctattac tttctttgga tttttttgat acattaatac acgacgtcca ataatagcat
    11701 caaaactttt tgtttcaaag gaggaaagtg ctgctaaatc tgcattttga taactaacat
    11761 tagcatagcc attatcatgt ctagcttgcc ataaaagttg ctcattaata tcaattccta
    11821 caacatcacc ttctttacca acgatatcag cagccaaacg cgttaattcc ccactaccac
    11881 aaccaatatc cataactctc ataccgggct gcaaagcttt ttttaataat gcttttgaaa
    11941 aatcttctct gtgaaacata ataacactct cctctcttct attttagcaa attaaaggtt
    12001 catacgtact atcagcaaaa aattataata ggttgaaatt ttatagcatt attttcttgt
    12061 tttcgtatct actttattat agaataacaa ctaatcattt ttagcatgac gagctcaatt
    12121 acaaaaggat aatcatgaaa aactatcgaa aacttattgt actactactt ctaatctttt
    12181 ttgccatttt tatgggagca tatgcttaca cgcatattgt tgaaaaaaga tccctaacta
    12241 gcaatactat tgaaaaaact ctacctgtgg taaatcagat taagcctcaa accattaaag
    12301 aataccaaaa ttacttaact aaggtagcta aacgtaatgt tcttcctgta gacattcctc
    12361 aggcattaaa taatgaaaag gtagaaatta ctgctactga tggcatgcaa acattcactt
    12421 ggaatgataa aaataatcct aagcaaaagg ttatcttcta tgttcatgga ggatcatata
    12481 tccatcaagc ttccgaatta caatatattt ttgtcaataa actagctaaa aaattagatg
    12541 caaaagttgt ctttcctatt taccctaaag ctcctacata taattatagt gatgctatcc
    12601 ccaaaattaa aaaattatac caaaatacat tagctagcgt cacatctcac aaacagatta
    12661 tcctagtagg tgaaagtgca ggcggaggcc ttgctttagg tattgctgat aaccttgtca
    12721 cggagcatat caaacaacca aaagaaatta ttttaatatc accatggtta gatattgcta
    12781 ctaataatcc aaagattgaa aaagttcaaa aaaaagatcc ccttcttaaa gcttggcaat
    12841 tacaacaagt agctccttac tgggcaaatg gaaaaaagaa ttttaaaaat cctcaagtta
    12901 gtcctcttta cagtagccaa tttaacaaga tggctccaat atcatttttt ataggaacgc
    12961 acgatatttt ctaccccgat aatcaattat tacatcaaaa attagcaaaa gaaaacatta
    13021 aacaccacta tatcgttggt caaaaaatga accacgttta tccagtctta ccgattcctg
    13081 aagcagagac tgctttcaaa caaatgctta aaattatcaa tcattaaata aaaaggtttc
    13141 caatcaagtt tagttggaaa ccttttttct attacttttg aatgactaat tatcttgaga
    13201 taaaaactct gagaataaaa cttcttgact aatcttttca gctaaagcct gtttaaaagc
    13261 taatttccct aaataatagg atttgtggtc aattgttggg agtttcaaaa gttgcccaac
    13321 taataaacat tcctgaccaa taatataagg acattttttc ctactttctt cataaccttt
    13381 tattagccct gccgcaactt catcacttgt agctaatata ccatctatac taacttcttt
    13441 taccaattga taagataaat ttagtccatc attaaagtca tgaacattcc caactaccat
    13501 atatggagag gatagttgcc catagacctc ttgataagct aataatgcgg attgataggt
    13561 agcagaggat tcattattcc ttgaaaataa taaaactaag tgctccaaac ctcgtaactt
    13621 catatcagaa aatgcctcta gaaatgaaga atagcgatca aggtaagcag aggatagatg
    13681 attatattcc tgcaattttt cacaaactac tattcgtcca tattttgcat aagtttcaat
    13741 aatgtcgagg ctaattgctc ttgaagtgaa tattaaagca tcaatagctt ccatcttgag
    13801 ctgtttcaag taggaaagtt ctaattcttg gttgtaatcc gaaggcatca caacaagttg
    13861 atagtcggta gttttagcag catctaatag gccatttatt aattgtgtaa aataaggatg
    13921 gcgcgtgtgg ggaatgacga cacctatttt ttgtgttttg cctcgactta aatcccgcgc
    13981 tagttgattt ggtatataat ccaattcggt gataagagcc attacttttt gtcgcgtctc
    14041 atcagaaaca tgtgggtgat gattgataac acgggaaacc gttgatttag caacacctgc
    14101 ctttttagct atatcactaa tacttgtcat tataaggctc ctactttaaa aatttttgaa
    14161 tcccatatcc cactccgtct tcatcattag acttagttag ttgatatgct atcgctttga
    14221 tatcatcaaa agcattatcc ataacaatag ggtaacctac catttcaagc ataggcaaat
    14281 cattatgtcc atctccaaat gcagccgttt cttctctagc taactgctct ttttgaagga
    14341 tgtaagcaat tcctttagat tttttggcta agagatgtgt aatttcaaga tacgctttac
    14401 ctgaacgttg aatagtgatt tctggtaact ccaaactttg aagatatttt tctaattcta
    14461 gcatatttgc ttcatcgaaa gtaatcatca taattttaaa agtatttgtg tgaccttcta
    14521 aaaactgatc ctcattatga ataaacgttg ggcattgttg agttaaacta tgttcgtatc
    14581 ggattccttc atctatttta tcacaatacc agttattaag atcgtaataa gataaactaa
    14641 cttgaggaaa atggaaccgt attcctctta gcaattgttt tactgtgctt tttttaatga
    14701 tttgtgtatg aataggtaga acttgattat tatctcctat tctataaatc aatcctccat
    14761 taaaagcaac ttggactcct cctaattgaa gtgcatctac ggcatccttc atttccatag
    14821 gtgccctagc agacactaaa gttactggaa tcgctgcctc acgtattaag gtagcgttac
    14881 ttttactcac ttgtccttca ctatttaata gtgtaccatc catgtcacaa aaaatacgtt
    14941 tgatagtcac aaaaaatacc tctcttcttc tgaaatcagt ataaatgctg gaacccgttc
    15001 catgtcaagc ttaaaattac tgaatccttg cgaaaaatag attgtattta acagatacgc
    15061 aatcatttta ataaatcaac gaactgtctg aatatgccac ttccaccatc ctgttagcct
    15121 aattgtacct gattgatccg ttcggaaaat cttgctttta atcttttgga acctttgtaa
    15181 agtctcttga tgaggatgct ggtaacgatt atttttacca gctgacacta gaaccacact
    15241 aggagacaac tttttcagaa aggatagact tgatgagccc ttagaaccat gatgtcctgc
    15301 tttaaggata tctactgata aattaggata agcttctaat aactgctttt ctccctcttc
    15361 ctccaaatca ccggtaaata agaagccttt tcctaataaa tgtccatata aaacaattga
    15421 atcattattt tttccatcac ccttgtgcca tgggtaaagt acttgtaaat aacttcccat
    15481 gacagaaagt ttatcaccag cttctataga ttttacagct actcctatct ttgataaacg
    15541 cttaacgtac tgcgaattcg ttaaagaacc tgaacttgta atcaaacgtg caactttaaa
    15601 atgctttgag ataacttcca tatcccccat atgatctgta tcggtatgag ttatcactaa
    15661 attatctatc ttgctaatac ctctgctttt caagtaagga atgagcgttc tctcagcatt
    15721 agactggttt actttttgtt tccattcttc aggctgttca aatctcaccc ttcccccagt
    15781 atcaattaaa attgtttttc ctaaccaatc cctcactaaa atactatctc cttgtccaac
    15841 atctaaaatc gcaacctcgt tagtaattgg attcttagtg ataaaaaaca aggtaaagat
    15901 aataagaagg gagcaataac gaaaacattt agaatgataa taatcataaa gtaatcccaa
    15961 aattataatc atgactatta aaagaaaaag gctgggcttt ccaaaaataa gtggtcttgg
    16021 aaacagttgc ccagtccatt ttaataatac ttctaggatt tcaaacaaac tattaataca
    16081 acttaattta acgaacggtg ataagacaaa tataacagtt aataaaggca aaaatataat
    16141 atcaaatgca aaagataaaa gtgctgttaa tataattgat attggttgaa aacccgaaaa
    16201 atagtaggtt agtatgggaa gaattcctac agagactgtc aaagattcga tagctacttt
    16261 ttttatactc gaaaaatgat caaaggaggt catcgtaagt ataaaagcat aagcaaagct
    16321 aagaactcct cccgtagtca aaagtgagtg ggggagagag ataagacaaa ttaacaagca
    16381 gagagcaaag ttctcatctt tcttgacacc gctatgtgat aataaagatt gaataagtgc
    16441 cctaacgact gagatactaa agcctgttaa accagcataa attagcgaga atggtatttg
    16501 aagtaaccaa acatggtcta gacgtaagcc aatacgtaga cagatataac gaaaaatacc
    16561 gagaaaaaaa cctacttgca tacctgacaa agcaaataaa tgtataatac ctagactact
    16621 atataaatcg gacatgtcat caaaggtctt atctagatat ccaaacaaaa gccctgacat
    16681 atagtgacgc ataggatttg gaaaagtttg actaataaca attgcacttc tacgccactt
    16741 aaacataaga tcaaagaaag acctatgttg aaataagcgt atctgtttta tctttgtcac
    16801 tttggcgata cgataaattc catgtctttt taaatactga cgataatcaa agccattaaa
    16861 atgccttctc tcctctgctt cttcaagttt gatatctgca attatcatta tattattatc
    16921 aagattttga aaataatctt tctcactttt attttttaat gtgtaaaaag cttgataagt
    16981 gttacctgaa gcatctgcct ggaatgataa aacatcacca ttgatagaaa tagtatcaat
    17041 acgcacttta atctggctaa tatgattcgg tactctttga
//
LOCUS       DBJYSH010000039        16891 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.039, whole genome shotgun sequence.
ACCESSION   DBJYSH010000039 DBJYSH010000000
VERSION     DBJYSH010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 16891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16891
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.039"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            5..78
                     /locus_tag="ACUBV3_000986"
     tRNA            5..78
                     /locus_tag="ACUBV3_000986"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Asn,seq:gtt)
     gene            complement(294..1646)
                     /locus_tag="ACUBV3_000987"
     CDS             complement(294..1646)
                     /locus_tag="ACUBV3_000987"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HHU3847495.1"
                     /translation="MKVVKFGGSSLASSQQLYKVLNIIKSDYTRRFVVVSAPGKRYEE
                     DLKMTDALIQYYQNYINGKDIVKDQTWIINRYQEIISDLSLGSTIAEEITRSIEQLAS
                     LPIENNQFLYDCFLAAGEDNNAKLVATFFNQNDIPARYVHPNEAGIIVTKEPCNARII
                     PGSYDKIENLCLYNEVLVIPGFFGVTEDNQICTFSRGGSDITGSLIAAGIKADLYENF
                     TDVDGIFAAHPGVVKNPHAIPELTYKEMRELAYAGFSVLHDEALLPAYRGRIPLVIKN
                     TNNPQQPGTKIVLKHTRSNIAVTGIASDSRFASINVSKYLMNREVGFGRKVLQILEDL
                     NISFEHMPTGIDDLSIVLREKELTPIKEQEILNYLTRKLEVDYVDIQHNLSTIVIVGE
                     NMKSQIGVTATATQALSREKINITMISQGSSEVSIMFVIDSKDEKRAIKALYETFFQK
                     "
     gene            1740..2390
                     /locus_tag="ACUBV3_000988"
     CDS             1740..2390
                     /locus_tag="ACUBV3_000988"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001900118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HHU3847496.1"
                     /translation="MCKYKAIIFDMDGVLFDTELFYYKRRERFLKQHGITIDHLPMNF
                     FIGGNMKQVWKSVLGDQYDTWDIDKLQQDYSRYKEDNPLPYKDLIFQDCKRVIEKLHH
                     KGYLLGLASSSTRHDIMLALESFNLDTYFKVILSGEEFSESKPNPAIYNRAAELLDIP
                     KQQILIVEDSEKGITAGIASGIDVWAIEDKYFGMNQSQANVLVSDLSQFFVKLDNY"
     gene            2527..3318
                     /locus_tag="ACUBV3_000989"
     CDS             2527..3318
                     /locus_tag="ACUBV3_000989"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000667362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HHU3847497.1"
                     /translation="MKFENIIYGIDGNVATIMLNRPDISNGFNIPMCQEIIDAIRLVS
                     ENKDVMFLVIEAQGPIFSIGGDLKVMKAAVESDDISSLTKIAELVNQISYDLLQLEKP
                     VVMCVDGAVAGAAANIALAADFVIASKKSKFIQAFVGVGLAPDAGGLLLLSKSIGITR
                     AVQLALTGESLSAEKAEALGIVYKLCESDKIGKIKDQLLKRLSRHSINSYQAIKSLAW
                     EAAFKDWEQYKKLELQLQESLAFKQDFKEGVRAHADRRRPNFLGK"
     gene            3414..3848
                     /locus_tag="ACUBV3_000990"
     CDS             3414..3848
                     /locus_tag="ACUBV3_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HHU3847498.1"
                     /translation="MEYDQINSYLVDIFNRIMIIEEMSLKTSQFSDVSLKEMHTIEII
                     GKHSEVTPSDVARELMLTLGTVTTSLNKLEKKGYIERKRSSIDRRVVHLSLTKRGRLL
                     DRLHSKFHKSMVSHIIEDLGEEDIKMLTSALGNLHKFLEDLV"
     gene            3848..4819
                     /locus_tag="ACUBV3_000991"
     CDS             3848..4819
                     /locus_tag="ACUBV3_000991"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HHU3847499.1"
                     /translation="MVFAKISQLAHYAPSQIIKNEDLSLIMDTSDDWISSRTGIKQRH
                     ISKNETTADLANKVAEQLIEKSGYSASQIDFIIVATMTPDSMMPSTAARVQAHIGASN
                     AFAFDLSAACSGFVFALSTAEKLISSGSYQKGLVIGAETVSKVLDWTDRGTAVLFGDG
                     AGGVLLEASKEKHFLAESLNTDGSRQGLQSSQVGLNSPFSDEVLDDKFLKMDGRAIFD
                     FAIKEVSKSINHLIETSHLEKEDIDYLFLHQANRRILDKMSRKIDIARDKFPENMMDY
                     GNTSAASIPILLSESYENGLLKLDGNQTILLSGFGGGLTWGSLIVKI"
     gene            4877..5101
                     /locus_tag="ACUBV3_000992"
     CDS             4877..5101
                     /locus_tag="ACUBV3_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HHU3847500.1"
                     /translation="MAVFEKVQEIIVEELGKDAEEVTLNTTFDDLDADSLDVFQVISE
                     IEDAFDIQIETEEGLNTVGDLVAYVEEKVK"
     gene            5255..6214
                     /gene="fabK"
                     /locus_tag="ACUBV3_000993"
     CDS             5255..6214
                     /gene="fabK"
                     /locus_tag="ACUBV3_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HHU3847501.1"
                     /translation="MKTRITELLNIKYPIFQGGMAWVADGDLAGAVSKAGGLGIIGGG
                     NAPKEVVKANIDKIKSMTDKPFGVNIMLLSPFVDDIVDLVIEEGVKVVTTGAGNPGKY
                     MERFHEAGITVIPVVPSVALAKRMEKLGADAIITEGMEAGGHIGKLTTMTLVRQVVDA
                     VTIPVIAAGGIADGRGAAAGFMLGADAVQIGTRFVVAKESNAHPNYKAKILKAKDIDT
                     AVSAQVVGHPVRALKNKLVTTYSQAEKDYLAGRISINEIEELGAGALRNAVVDGDVIN
                     GSVMAGQIAGLIKSEETCQEILEDIYSGARQVILSEANRWSDL"
     gene            6234..7160
                     /gene="fabD"
                     /locus_tag="ACUBV3_000994"
     CDS             6234..7160
                     /gene="fabD"
                     /locus_tag="ACUBV3_000994"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HHU3847502.1"
                     /translation="MNKVAFLFAGQGAQKLGMARDLYETFPIVKETFDKASHVLGYDL
                     RELIDKDLDKLNQTKYTQPAILTTSTAIYRLILKEIGLRPDMVAGLSLGEYSALVASG
                     AIRFEDAVVLVARRGQLMEAAAPAGSGKMVAVLNADRQIIEDACKKASQFGIVSPANY
                     NTPKQIVIGGESIAVNAAVEELKQQGVKRLIPLNVSGPFHTALLKPASQKLSDVLDKV
                     HFSVSEIPVIGNTEAQIMKKDDIKSLLARQVMEPVRFDESIETMKKMGMTQVVEIGPG
                     KVLSGFLKKIDSSLSVYSVEDKIGFNNLKELN"
     gene            7169..7903
                     /gene="fabG"
                     /locus_tag="ACUBV3_000995"
     CDS             7169..7903
                     /gene="fabG"
                     /locus_tag="ACUBV3_000995"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HHU3847503.1"
                     /translation="MQLKDKNIFITGSSRGIGLAIAHQFAQLGANIVLNGRSEISEDL
                     IAEFADYGVKVIAISGDVSSFEDANRMIKEAIASLGSVDVLVNNAGITNDKLMLKMTV
                     EDFESVLKINLTGAFNMTQSVLKPMTKARQGAIINISSVVGLTGNVGQANYAASKAGL
                     IGFTKSVAREVAARGIRVNAIAPGFIESDMTDVIPEKMQEAILAQIPMKRIGKGKEVA
                     QVASFLAEQEYLTGQVIAIDGGMTMQ"
     gene            7919..9151
                     /gene="fabF"
                     /locus_tag="ACUBV3_000996"
     CDS             7919..9151
                     /gene="fabF"
                     /locus_tag="ACUBV3_000996"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HHU3847504.1"
                     /translation="MTLQRVVVTGYGVTSPIGNTPEEFWNSLKEGNVGIGPITKFDSS
                     DFMVKNAAEIHDFPFDKYFVKKDLNRFDMYSLYALYASSEAIQHANLNLDEIDADRFG
                     VIVASGIGGIQEIEEQVIRLHEKGPKRVKPMTLPKALPNMAAGNVAMRLGAHGVCKSI
                     NTACASSNDAIGDAFRNIKFGIQDIMVVGGAEAAITKFAIAGFQSLTALSTTEDPSRA
                     SIPFDKDRNGFIMGEGSGMLVLESLGHAEKRGATILAEVVGYGNTCDAYHMTSPHPEG
                     LGATKAIQLALAEANIKPEEVNYVNAHGTSTPANEKGESQAVVAALGTDVPVSSTKSF
                     TGHLLGAAGAVEAIATIEAIRHSYVPMTAGTTELSEDITANVIFGQGQDADIRYAISN
                     TFGFGGHNAVLAFKRWED"
     gene            9153..9653
                     /gene="accB"
                     /locus_tag="ACUBV3_000997"
     CDS             9153..9653
                     /gene="accB"
                     /locus_tag="ACUBV3_000997"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HHU3847505.1"
                     /translation="MDIQEIKDLMTQFDESSLREFSFKTSDGELSFSKNEGKAPLVPT
                     MSHMSHQPEATPTIATPVSNEAGEQTKQATEVVSEIPESTVTVAEGDVVESPLVGVAY
                     LASGPDKPNFVSVGDSVKKGQTLMIIEAMKVMNEVPAPHDGVVTEILVANEEVIEFGK
                     GLVRIK"
     gene            9650..10072
                     /gene="fabZ"
                     /locus_tag="ACUBV3_000998"
     CDS             9650..10072
                     /gene="fabZ"
                     /locus_tag="ACUBV3_000998"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0019171 -
                     (3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HHU3847506.1"
                     /translation="MIDIKEIREALPHRYPMLLVDRVLEVSEDEIVAIKNVSINEPFF
                     NGHFPEYPVMPGVLIMEALAQTAGVLELSKEENKGKLVFYAGMDKVKFKKQVVPGDQL
                     VMTAKFVKRRGTIAVVEAVAEVDGKLAASGTLTFAIGN"
     gene            10110..11480
                     /gene="accC"
                     /locus_tag="ACUBV3_000999"
     CDS             10110..11480
                     /gene="accC"
                     /locus_tag="ACUBV3_000999"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HHU3847507.1"
                     /translation="MFKKILIANRGEIAVRIIRAAREMGISTVAIYSEADKESLHTIL
                     ADEAICVGPAKSAESYLNVNAILSAAIVTGAEAVHPGFGFLSENSKFATMCEEMNLKF
                     IGPSGEVMDKMGDKINARAEMIKADVPVIPGSDGQVTSVEEAVSIAEEIGYPLMLKAS
                     AGGGGKGIRKVKSADELKPAFESASQEALAAFGNGAMYIEKVIYPARHIEVQILGDSF
                     GKIVHLGERDCSLQRNNQKVLEESPSVAIGNTLRQQIGEAAVRAAEAVSYENAGTIEF
                     LLDENSGQFYFMEMNTRVQVEHPVTEFVTGVDIVKEQIRIAAGIPLSVSQNDIKLTGH
                     AIECRINAENPQFNFAPCPGTINGLHLPAGGMGLRVDSAVYTGYTIPPYYDSMIAKVI
                     VHGENRFDALMKMQRALYELEIDGIVTNTEFQMDLISDKKVLAGDYDTSFLMEDFLPR
                     YYGNNQ"
     gene            11489..12364
                     /gene="accD"
                     /locus_tag="ACUBV3_001000"
     CDS             11489..12364
                     /gene="accD"
                     /locus_tag="ACUBV3_001000"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HHU3847508.1"
                     /translation="MALFSKKDKYIRISPNKALGSSDKRSLPEVPDELFAKCPSCKHM
                     IYQKDLGLAKICPACSYNFRISAQERLLLTVDEDSFEELFTGIETKDPLNFPNYREKL
                     AATRQKTNLDEAVVTGLAKIKGQTTALAIMDSHFIMASMGTVVGEKLTRLFELATEKK
                     LPIVIFTASGGARMQEGIMSLMQMAKVSAAVKRHSNQGLFYLTILTDPTTGGVTASFA
                     MEGDIILAEPQALVGFAGRRVIETTVREDLPEDFQKAEFLLEHGFVDAIINRTELRDC
                     IAQLIAFHGNEVGHE"
     gene            12357..13130
                     /locus_tag="ACUBV3_001001"
     CDS             12357..13130
                     /locus_tag="ACUBV3_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001058273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HHU3847509.1"
                     /translation="MNNDVTRILKDARDQGRLTALDYAELIFDNFMELHGDRQFADDK
                     SIIGGLGYLAGRPVTIVGIQKGKNLQDNLDRHFGQPHPEGYRKALRLMKQAEKFGRPV
                     ITFINTAGAYPGVGAEERGQGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALAVAD
                     KVWMLEHTVYSILSPEGFASILWKDGTRTTEAAQLMKMTAGELYHMEVVDKVIPEHGY
                     FSSEIVDMIKTSLISELEMLSQLSLEDLLEQRYQRFRKY"
     gene            13600..14232
                     /locus_tag="ACUBV3_001002"
     CDS             13600..14232
                     /locus_tag="ACUBV3_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000284161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI_0552 family protein"
                     /protein_id="HHU3847510.1"
                     /translation="MYQELNDYLEFQSMKYIMPEKAGPLKDDMQTFKAKGQLARNSFT
                     ELSRALEQRMDGFKMQRVSNWANQAQVGRPHFWVYYRKDTDQLDDVAVALRVYGVKDS
                     FGVSLEVSFVERQKSDKTLEKQARVLSIPIASPLYFMVQRQGETHREEGNEENRQRLM
                     QEIKSGKVRKVLVKYDVLLTENQSLENILQRLLEGFEKVLPYYQVCQTIS"
     gene            complement(14278..15555)
                     /gene="serS"
                     /locus_tag="ACUBV3_001003"
     CDS             complement(14278..15555)
                     /gene="serS"
                     /locus_tag="ACUBV3_001003"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HHU3847511.1"
                     /translation="MLDLKRIRTDFDAVAKKLAMRGVDQETLTTLKELDIKRRELLIK
                     AEEAKAQRNVASAAIAQAKRNKENADEQIAAMQTLSADIKAIDAELADVDANLQSMVT
                     VLPNTPADDVPLGADEDENVEVRRWGTPREFDFETKAHWDLGESLGILDWERGAKVTG
                     SRFLFYKGLGARLERAIYSFMLDEHAKEGYTEVIPPYMVNHDSMFGTGQYPKFKEDTF
                     ELADSPFVLIPTAEVPLTNYYRDEIIDGKELPIYFTAMSPSFRSEAGSAGRDTRGLIR
                     LHQFHKVEMVKFAKPEESYQELEKMTANAENILQKLNLPYRVITLCTGDMGFSAAKTY
                     DLEVWIPAQNTYREISSCSNTEDFQARRAQIRYRDEVDGKVRLLHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTIPEVLRPYMGNIDIIKPN"
     gene            15854..>16891
                     /locus_tag="ACUBV3_001004"
     CDS             15854..>16891
                     /locus_tag="ACUBV3_001004"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016413 - O-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HHU3847512.1"
                     /translation="MEKYTSIKQSGRHYGLDLLRIISMFMIVITHILGKGGLRSSVEG
                     HADSYFIVTWIIQVLVYGAVNCYALISGYVGINSRYRYSKLLSIWAQVFFYTFTITAL
                     FEITGHEVTLLNWRDAFFPIVSGQYWYITAYFGLLVFMPVINNGLNALTDKQLKQLVL
                     LMFIIFSILPAVLNNRVPEFSLSKGFEMTWLLILYIIGAYLKRIDLNIFKTSYLLIVY
                     LLSLVATYVMKFSVGDIWYWYVSPTLTLGAVSLFILFARASIKPSGFLKKIIVVLAPS
                     TLGVYLCHLHPLIVKYFVRDFAETFVYESIYLYPFLILGAGILIYLLAFIVERIRLRI
                     FKAIHFSNFLRW"
BASE COUNT         5354 a         2585 c         3534 g         5418 t
ORIGIN      
        1 ataatccgcc atagctcagt tggtagtagc gcatgactgt taatcatgat gtcgtaggtt
       61 cgagtcctac tggcggagta gataaaggag actaactttg gttagccttt ttttattgga
      121 attattattc cttaaggttg gctagcatct taattgtccg aatttgtcag tgataccaag
      181 tagttacgat taaaattgaa aaaactatcc tatgttctaa ataaaatcgt cctaagtaaa
      241 cttacttagg acgatttgtt atctatctaa tagtgtaagt agtgtaatag gtactatttt
      301 tggaagaatg tttcatatag tgctttaata gctctttttt catccttact gtctataacg
      361 aacataatgg agacttcgct tgaaccttgt gatatcatgg tgatattgat tttttctctt
      421 gataaggctt gtgtcgctgt tgcagtgact ccaatctgac ttttcatatt ttcacctaca
      481 attacgattg tagataaatt gtgttggatg tcaacgtaat ctacttctag tttacgagtt
      541 aggtaattta agatttcttg ttctttgatt ggtgtcaatt ctttttcacg taagacaatg
      601 gatagatcat ctatgccagt tggcatatgt tcaaaactaa tatttaaatc ctctaaaatt
      661 tgtagtactt ttcggccgaa acctacttct ctattcatta agtatttaga tacgtttatg
      721 ctagcaaaac ggctatcaga agcgatccca gttactgcta tgttactacg agtatgcttt
      781 aaaactattt ttgtaccagg ctgttgggga ttatttgtat ttttaataac aagagggatt
      841 ctgccacgat aggcaggaag taaagcttca tcatgtaaaa ccgaaaaacc cgcataggct
      901 aattcacgca tttctttata agtaagctca gggatagcgt gagggttctt aactacacct
      961 ggatgtgctg caaatatacc atcaacatct gtgaagttct cataaaggtc tgcttttatg
     1021 cctgctgcaa ttagggatcc agtaatgtca gatccccctc ttgaaaaggt acaaatttgg
     1081 ttatcttctg tgactccaaa aaatccaggg ataacaagaa cctcattgta tagacataag
     1141 ttctcaatct tatcataact tcctggaata attcgtgcat tacatggttc tttagttaca
     1201 ataattcctg cttcgtttgg atgaacatat cttgcaggaa tgtcattttg attaaaaaac
     1261 gttgcaacta actttgcgtt gttatcttca ccggctgcta aaaaacaatc atataaaaac
     1321 tgattatttt ctatgggaag tgaagctagc tgctctatag agcgagtaat ttcttctgca
     1381 atagtgcttc ctaaagacaa atcactaata atttcttgat aacgattgat aatccaagtt
     1441 tggtctttaa caatatcttt accgttaata taattttgat aatattgtat caaagcatct
     1501 gtcattttta agtcttcttc gtagcgtttc cctggagctg aaacgacaac aaaccgtcta
     1561 gtgtagtctg atttgataat atttagtact ttgtataatt gttgggaaga tgctaaagaa
     1621 cttcctccaa atttgactac tttcataggt tacctcaaga atgaatttta gataaattat
     1681 aacaaaagtc agaaaatttg tcattacctc taaatttaaa agaatgatat aatagttaca
     1741 tgtgtaaata taaagcaata atatttgata tggatggtgt tctatttgac actgaattat
     1801 tttattataa aaggcgagag cgttttttaa aacaacatgg aattacaatt gaccatttac
     1861 caatgaattt ttttattgga ggaaatatga agcaggtttg gaaatctgtt ttaggtgatc
     1921 aatatgatac ctgggatatt gataagctcc aacaagatta tagtcgctat aaagaggata
     1981 atcccttgcc atacaaagat ttaatttttc aggattgtaa aagagtcatt gaaaagcttc
     2041 atcacaaagg ctatcttttg ggtctagctt caagttctac tagacatgat atcatgttag
     2101 cattagaaag ttttaatctg gatacatatt ttaaggtaat tctgtctggt gaggaatttt
     2161 cagaaagtaa acctaatcca gctatttata atagagcagc agaactttta gatattccta
     2221 aacagcaaat tcttattgta gaagacagtg agaagggtat tacagctggt attgcatccg
     2281 gtattgatgt gtgggctatt gaagataaat actttggaat gaatcagtcg caggctaatg
     2341 tattagtgag cgatctgagt caattttttg tgaaattaga taactattaa aaacgggaag
     2401 tttaattccg tttttttatt ttttatcttg ccatgaaagc taggtttatg ataaaataca
     2461 aagcatgata atttgataat caaattattt tttatttttc tttcactctt aaagagagga
     2521 gacattttga agttcgaaaa tataatatat ggtattgatg gcaacgtggc cactattatg
     2581 cttaatcgac cagatatttc aaatggtttt aacatcccaa tgtgtcaaga aattattgat
     2641 gcaataaggc ttgtgtctga aaataaagat gtcatgtttt tagtgattga agcacaagga
     2701 ccaatctttt caatcggagg agatttaaaa gtgatgaaag ctgctgttga gagcgatgat
     2761 attagctcat taactaaaat tgctgagtta gttaatcaga tatcgtatga tttactacag
     2821 ctagagaaac ctgttgttat gtgtgtagat ggggctgtag ctggtgccgc agctaatatt
     2881 gcattagcag ctgattttgt tatcgcaagc aagaagagta aatttatcca ggcttttgtg
     2941 ggtgtcggtt tagctccaga tgcaggaggt ttattgttac ttagtaaatc aatcggtata
     3001 actcgtgcag tgcaactagc tttaactgga gaaagcttat ctgctgaaaa agctgaagct
     3061 ttgggaattg tttataaact ttgtgaatca gataaaatag gtaaaattaa ggatcaacta
     3121 ttaaagcgtt taagtagaca ttctataaac tcatatcaag ccataaaatc attagcttgg
     3181 gaagctgctt ttaaagattg ggaacaatat aaaaagcttg aattacagtt acaagaatca
     3241 ttagccttta agcaagattt taaagaaggt gttcgggcac acgctgatag aaggcgtcct
     3301 aattttttag gtaaatagtt tgaaaatcaa attatttaag attaaagtct tgcaaataag
     3361 tttgaacttt gatataattt gattatcaaa gtttaatgtg aggtggtgat atcttggaat
     3421 atgatcagat taatagttat ttagttgaca tttttaatag aatcatgatt attgaggaaa
     3481 tgagtttaaa gactagccaa tttagcgatg tatctttaaa agaaatgcat actattgaaa
     3541 ttattgggaa acattctgag gtaactccta gcgatgtcgc tcgagaatta atgttaactt
     3601 tgggaactgt aacaaccagc cttaataagt tagaaaaaaa aggctacatt gagcgtaaac
     3661 ggtcaagtat tgatcgtcga gttgttcact tgagtcttac taagagaggt aggttattag
     3721 atcgattgca tagtaaattt cataaatcta tggtatctca tattattgaa gacctaggtg
     3781 aagaagacat aaaaatgttg acgagcgctt tagggaatct tcataaattt ttggaggact
     3841 tagtctagtg gtttttgcta aaattagtca acttgcacat tatgcacctt ctcaaattat
     3901 taaaaatgag gatttatcat taatcatgga taccagtgat gactggattt cgtcacgtac
     3961 tggtataaaa caacgtcata ttagtaaaaa tgaaacaaca gctgatttgg caaataaggt
     4021 cgcagaacaa cttattgaaa aatccggtta ttccgctagt caaattgact ttattattgt
     4081 tgcaacgatg acaccagact ctatgatgcc ttctactgcc gcaagagtgc aagctcatat
     4141 tggagcgagt aatgcttttg cctttgactt gagtgcagca tgtagtggtt ttgtttttgc
     4201 cttgtctacc gccgaaaaat tgatatcatc aggtagctat caaaaaggtt tagtaatagg
     4261 agcagaaact gtttctaaag tattagactg gactgataga gggacagcag ttttatttgg
     4321 agatggagct ggaggtgtct tgctagaagc ttctaaggaa aaacattttc ttgcagagtc
     4381 tctcaataca gatggtagtc gtcagggact tcaatctagt caagtcgggt taaattcacc
     4441 tttctcagat gaagtacttg atgataaatt tttgaaaatg gatggtagag caatctttga
     4501 ttttgctatc aaagaagttt caaaaagtat caatcatctt atagaaacat cccatttgga
     4561 aaaagaagat attgattatc ttttcttaca ccaagcgaat cgaaggattt tagataagat
     4621 gtctcgaaaa attgatatag cacgtgataa atttccagag aatatgatgg attatggtaa
     4681 tactagtgca gctagtatcc ctattttact ttcagaatct tatgagaacg gtttgttgaa
     4741 actagatggt aaccaaacaa tcctcttatc gggtttcggt ggaggtttga catggggaag
     4801 tctaattgtt aaaatttagt tatatatagt gcatagcaca tttataaaag attatttaaa
     4861 atttaaggag ctaaaaatgg cagtatttga aaaagtacaa gaaattattg ttgaagaact
     4921 tggtaaagat gcagaagaag taacgttgaa cactactttt gacgatcttg atgctgattc
     4981 gcttgatgtt ttccaagtga tttcagaaat tgaagatgca tttgatatcc aaattgagac
     5041 agaagaagga ctaaatactg ttggtgattt agttgcttat gttgaagaaa aagtaaaata
     5101 attaagtcgt aagaaaaaag aagcaagtga taataaaaga tattcttacc aaatcactta
     5161 ttagagtgag tattcaatca atcgttgatc ggtactcact cttcttctta tcaatatagt
     5221 ttgattatca aattattaat tttaggaaag aaaaatgaaa acacgtataa cagaattact
     5281 aaatataaaa tatccaattt ttcaaggggg tatggcgtgg gtcgctgatg gagaccttgc
     5341 aggagcagtt tctaaggcag gtggtctagg tattattggt ggaggaaatg cccctaaaga
     5401 ggttgtcaaa gcaaatattg ataaaataaa atctatgact gataaaccct ttggtgtgaa
     5461 tattatgctt ttatcacctt ttgtagatga tattgttgat ttggttattg aagaaggtgt
     5521 aaaagttgta acgacgggtg caggtaatcc tggtaagtac atggagcgct ttcatgaagc
     5581 aggtataaca gtaatccctg ttgttcctag tgttgcatta gcaaaacgta tggagaaatt
     5641 aggggctgat gctatcatta cagaaggaat ggaagcggga ggtcacatcg ggaaactgac
     5701 cacgatgaca ttagtgcggc aggttgttga tgctgttaca attccagtca ttgccgcagg
     5761 tggtatagca gatggacgtg gggctgctgc gggtttcatg ttaggagctg atgctgttca
     5821 gataggaact cgttttgttg ttgctaaaga atctaatgct caccctaatt acaaagcaaa
     5881 aatcttaaaa gcaaaagata tcgacactgc tgtttctgct caggtagtag gtcacccagt
     5941 tcgtgcctta aaaaataaac tagtaacaac ctatagccaa gcagaaaaag attatttagc
     6001 tggccgtatt tcaatcaatg agattgaaga attaggagca ggagctcttc gcaatgcagt
     6061 tgttgatggg gatgttatta atggttcagt tatggcagga caaattgccg gattaattaa
     6121 atcagaagag acttgtcaag agattcttga agatatttat tcaggagcgc gtcaagttat
     6181 tctttctgag gcaaaccgct ggtcagattt ataaatacaa ataaggagac actatgaata
     6241 aagtcgcatt tctatttgct ggtcaaggtg ctcaaaagtt gggaatggct agagatttat
     6301 atgaaacttt cccgattgtt aaagaaactt ttgataaggc aagtcatgtt ttaggatacg
     6361 atttgagaga attgattgat aaagacttag acaaattaaa tcagacgaaa tatactcaac
     6421 cagctatttt aacgacttcg actgctattt atcgtttaat tcttaaagaa attggactta
     6481 gaccagatat ggttgcagga ctttctttgg gagaatacag tgctttagtt gcttcaggtg
     6541 ctataagatt tgaagatgct gtagtacttg ttgctagacg aggtcaacta atggaagcag
     6601 cagcacctgc tggtagtggc aaaatggtag cggttttaaa tgctgatcgt caaataattg
     6661 aagatgcttg taagaaggct tctcagtttg gtattgttag tcctgcaaat tataatactc
     6721 caaagcaaat tgttataggg ggagagtcaa tcgctgtaaa tgcagctgta gaagaactaa
     6781 aacaacaagg agtaaaacgt ttaattcctc tgaatgtttc aggtcctttt cacactgctt
     6841 tattgaaacc tgcaagtcag aaattgagcg atgttttaga taaagttcat ttttcagttt
     6901 ctgaaatccc tgtaattgga aatacagagg cgcagataat gaaaaaagac gatatcaaat
     6961 ctttattggc acgccaagtt atggaacctg tacgctttga tgagtctatt gaaaccatga
     7021 aaaaaatggg aatgacacag gttgttgaaa ttggtccagg gaaggtactc tcaggtttct
     7081 tgaaaaaaat agatagctca ttatcagtat attctgttga agataaaatt ggatttaata
     7141 atcttaagga attaaactag gggaaaatat gcaattaaaa gataagaaca tttttattac
     7201 gggttcaagt agaggaatcg gccttgcaat tgcccatcaa tttgcacaat taggtgctaa
     7261 tattgtttta aatggacgtt ctgaaatatc agaagactta atagctgaat ttgctgatta
     7321 tggtgtaaaa gttattgcta tttcaggaga tgtttcttct tttgaagatg ctaatcgcat
     7381 gattaaagaa gctattgcaa gtctaggtag cgtggatgtg ctcgttaaca acgctggtat
     7441 tactaatgat aaattgatgc ttaaaatgac tgttgaagat tttgagagcg ttcttaaaat
     7501 taatttaaca ggtgcattta atatgacaca atcagtcttg aaaccgatga caaaagcgcg
     7561 tcaaggtgct attattaata tctctagcgt cgttggatta actggaaatg tagggcaagc
     7621 taattacgca gcctctaaag caggtttaat tggctttaca aaatcagttg ccagagaagt
     7681 agcagcacga ggcattcgtg tcaatgccat tgctccaggc tttatcgaat ctgatatgac
     7741 agatgttatc ccagaaaaaa tgcaagaagc tattttagct caaattccaa tgaaacgtat
     7801 tggaaaaggt aaagaagtag cgcaggtagc gtctttctta gcagaacaag aataccttac
     7861 aggacaagtt attgccattg atggcggtat gacgatgcaa taataaagag gaaataatat
     7921 gacattacag cgagtagtag taacaggtta cggtgtaacc tcacctattg gaaacacacc
     7981 agaagaattt tggaatagcc ttaaagaagg aaatgttggt attggaccta tcactaaatt
     8041 tgattcaagt gatttcatgg tcaaaaatgc tgctgaaatt catgattttc catttgataa
     8101 atattttgtc aaaaaagatc tgaacagatt tgatatgtat tccctttatg ccttatatgc
     8161 ttcaagtgaa gctattcaac atgctaattt aaatttagat gaaattgatg cagatcgatt
     8221 tggtgtcatc gttgcatcag gtattggagg gattcaagag attgaagaac aagtgattcg
     8281 tcttcatgaa aaagggccaa aacgcgtaaa accaatgacg cttccaaaag ctctaccaaa
     8341 tatggcagct ggaaatgttg cgatgaggtt aggcgctcat ggtgtatgta aatcaattaa
     8401 tacagcttgt gcttcttcta atgatgctat tggagatgct ttccgtaata taaaatttgg
     8461 tattcaagat attatggtag tcggtggtgc agaagctgct attacaaaat ttgccattgc
     8521 tggattccaa agtcttacag cattatcaac aactgaagat ccatcaagag catctattcc
     8581 atttgataaa gatcgcaatg gatttatcat gggagaaggt tctggaatgt tggtcttaga
     8641 aagtttagga cacgctgaaa aacgaggagc tactattctt gcagaagttg ttggttatgg
     8701 taacacttgt gacgcatatc atatgacttc accacatcca gaaggtttag gagccactaa
     8761 agctattcaa cttgcactgg ctgaagctaa tattaaacct gaagaagtta actatgtaaa
     8821 tgctcatgga acttcaacac ctgctaacga aaaaggagag agtcaagctg ttgttgctgc
     8881 acttgggaca gacgttccag tttcttcgac taaatctttt actggtcatc ttttgggtgc
     8941 cgcaggagct gttgaggcaa ttgcaactat tgaagcgata cgtcatagtt atgttccaat
     9001 gactgctgga acaacagaat tatcagaaga tattacagcg aatgttattt ttggtcaagg
     9061 gcaagacgct gatattcgtt atgccatttc aaatacattt ggttttggag gacacaatgc
     9121 agttctggcc ttcaaacgct gggaggatta aattggatat tcaagagatt aaggatttaa
     9181 tgactcaatt tgatgagtca agtctgagag aattttcatt caaaacaagt gacggtgagc
     9241 tgtcttttag taaaaatgag ggaaaagctc cacttgtccc aactatgtct catatgagtc
     9301 atcaacctga agcaactcca actatagcaa ctcctgtctc taatgaagca ggtgagcaaa
     9361 cgaaacaagc tactgaagta gtttcagaaa tcccagagtc gactgtgact gttgcagaag
     9421 gtgacgttgt cgaaagtcct ttagttgggg ttgcttattt agcttctgga ccagataaac
     9481 ctaactttgt ttcagtaggt gatagcgtga agaagggaca aactttgatg attattgagg
     9541 cgatgaaggt gatgaatgaa gtgcctgctc ctcatgatgg tgtagttact gaaattcttg
     9601 tagccaatga agaagttatt gaatttggta aaggattggt acgaatcaaa tgattgatat
     9661 aaaagaaatt cgtgaggctt tacctcaccg ctatccaatg ttattagtgg atcgtgtttt
     9721 agaggtatca gaagatgaga tagtcgctat caaaaatgtt agtatcaatg agcctttttt
     9781 caatggtcat tttccagaat atcctgtaat gccaggcgtt ttaattatgg aagctttggc
     9841 tcaaactgca ggtgttttag aattatctaa agaagaaaac aaaggaaagc ttgtttttta
     9901 tgcaggtatg gataaagtga aatttaaaaa gcaagttgtt ccaggagatc aattagttat
     9961 gactgctaag tttgtcaaac gtagaggaac tatagccgtt gttgaagcag tagcagaagt
    10021 tgatgggaaa ttagctgcca gtggaacgtt aacatttgca ataggcaatt gaagatagta
    10081 ttgaatttag agggagagaa gcatcaaaaa tgtttaagaa aatattgatt gctaatcgcg
    10141 gagaaattgc agtacgtatc attcgtgcag cccgtgaaat gggaatttcg acagttgcta
    10201 tctattcgga ggctgacaag gaatcattgc acacaatttt agcagatgaa gctatttgtg
    10261 ttggaccagc caaatcagca gaatcttatt taaacgttaa tgctatatta tctgctgcta
    10321 ttgtgactgg agcggaagct gttcatccag gttttgggtt tttaagtgaa aattctaaat
    10381 ttgctactat gtgtgaagag atgaatttaa aattcattgg tccatctgga gaagttatgg
    10441 ataaaatggg tgataaaatc aatgctcgtg cagagatgat aaaagctgat gttccggtta
    10501 tcccaggatc agatggtcaa gttacaagtg tagaagaagc tgtatcaatt gctgaggaaa
    10561 tcggctatcc attgatgtta aaagcctcag ctggtggcgg tggaaaagga attaggaagg
    10621 tcaaatcagc agatgaattg aagcctgctt ttgagtctgc ttcacaggag gctttggctg
    10681 cgttcggtaa tggtgctatg tatattgaaa aagttatata cccagctaga catattgaag
    10741 tacaaatact tggagatagc tttggtaaga tagttcacct aggagaacgt gattgttctc
    10801 tgcagcgtaa caatcagaag gtattggaag aaagtccgtc tgtggcaata ggtaacactc
    10861 tgcgtcaaca aattggtgaa gcagctgttc gtgcagctga ggctgtatcc tatgaaaatg
    10921 caggaacaat tgagtttttg ttagatgaga atagcggaca gttctatttc atggaaatga
    10981 atactcgtgt tcaagtagaa caccctgtta cagaatttgt aacgggtgtt gacatcgtta
    11041 aagagcagat tcgtatcgct gctggaattc ctttatcagt ttctcaaaat gatattaagt
    11101 taactggtca tgcgattgaa tgtcgtatta acgcagagaa tccacaattt aattttgcgc
    11161 catgtccagg taccattaat gggttacatc tacctgctgg cggtatgggc ttacgtgtcg
    11221 attcagcggt ttacacaggc tacactattc ctccttatta tgatagtatg attgcgaaag
    11281 taattgttca cggagaaaat cgttttgatg cacttatgaa aatgcaacgt gccttatatg
    11341 agcttgaaat tgatggtatt gtaacaaata cagaatttca aatggatctc atttctgata
    11401 aaaaagtctt ggctggggat tatgatactt cattcctgat ggaagacttt ttaccaaggt
    11461 attatggtaa caatcaatag gagggaagat ggctttattt agtaaaaagg ataaatatat
    11521 ccgtattagt ccaaacaaag cattggggtc ttctgataaa cgtagtcttc ctgaggttcc
    11581 tgatgaactc tttgctaagt gtccttcttg taaacatatg atttatcaga aagatttagg
    11641 attagcaaag atttgtccag cttgttctta caactttaga atttcagcgc aagaacgtct
    11701 gttgctaaca gttgacgaag atagttttga ggaattattt acagggattg aaactaagga
    11761 tcctttgaat tttccaaact accgtgaaaa actggctgct acacgtcaaa agactaactt
    11821 agatgaagcg gtggtgacag gtttagctaa aataaaagga caaactacgg ccttagctat
    11881 tatggattct cattttataa tggcgtctat gggaacggtc gtaggcgaaa agttgacaag
    11941 attatttgag ctagcaactg aaaagaaatt acctattgtt atttttacag cgtcaggagg
    12001 cgccagaatg caagaaggta ttatgagttt gatgcaaatg gcaaaagtat cggcagcagt
    12061 taaacgccat tctaaccagg gattgtttta tttaacaatt ttaacagacc caacaaccgg
    12121 cggcgttact gcgagttttg ctatggaagg tgatattatt ctggctgagc cgcaagcgtt
    12181 ggttggtttt gctggtcgtc gtgttatcga aacaacggtg agagaagatt tacctgaaga
    12241 ctttcaaaag gcagaatttt tgttagaaca tggttttgtc gatgctatta taaatcgaac
    12301 agaattacgc gactgcattg ctcaactaat tgctttccat ggaaatgagg ttggtcatga
    12361 ataatgatgt gacaagaatt ttaaaagatg cgcgtgatca agggaggcta actgctcttg
    12421 actacgctga attgattttt gataatttta tggaacttca tggtgatcgc cagtttgcag
    12481 atgacaaatc aattatagga ggtttggggt acttggcagg gcgtcctgtg acgattgtcg
    12541 gtattcaaaa aggtaagaac cttcaagata atttagaccg tcattttggt caaccacatc
    12601 ctgaaggtta tcgtaaggct ttacgtttaa tgaaacaagc tgagaagttt ggaagacctg
    12661 ttataacctt catcaatact gctggagcat atcctggggt aggtgctgag gagcgaggtc
    12721 agggtgaagc aattgctaga aacctcttag aaatgagtga tttaaaagtc cctattatag
    12781 ctattattat tggtgaaggg ggttctggag gagcattagc tttagctgtt gccgataagg
    12841 tttggatgtt ggaacatact gtttattcta ttttaagtcc tgagggcttt gcttctattt
    12901 tatggaaaga tggcacaaga acaacagaag cagcacaatt aatgaagatg actgctggag
    12961 aactttacca tatggaagtt gtagacaaag taatccctga acatggttat ttttcaagtg
    13021 agattgtcga tatgattaag acaagtttaa tttcagagtt agaaatgctc tcacaattat
    13081 ctcttgagga tttattggaa caacgttacc aacgttttag aaaatattaa tagaaaaagg
    13141 ctgagttatt tactcagcct atatttagtt tttcaaggta ttatcatagt tatagtttca
    13201 tattttgcta taattgataa aaaggagata tgtcaatgac actagcatta gccaattttt
    13261 agattgtagt ctaatccaaa attttcagaa agagtcattt cgaacacttt attctaagta
    13321 ccattattta ggctctcctt aacttgagac tctatctgtt attgaagaga aattttctag
    13381 aaaaaatagc tattattaga ttttagaagg ctatgaaaag attggttttt aaagagttcc
    13441 attaagtgat gaagaaagta aagcttggtt agttataatt gcgattagac cttgtttcta
    13501 gcaacgaggc ttcgctagaa atactttaaa agaacttgaa agtatgtata gatatatata
    13561 atcgtgaaaa cttaactatt gtttacatgg agaaaaagaa tgtatcaaga attaaatgat
    13621 tatttagaat tccaatcaat gaagtatatt atgcctgaga aggcaggccc tttaaaggac
    13681 gatatgcaaa cttttaaggc aaagggccaa cttgctagga atagttttac agagttatca
    13741 agagccttag aacaacgtat ggatggcttt aaaatgcaaa gagtgagtaa ttgggctaat
    13801 caagctcaag tagggcgtcc acatttttgg gtttattatc gaaaggatac agaccaatta
    13861 gatgatgtgg ctgttgcgct aagggtttat ggtgtaaaag atagttttgg ggttagtctt
    13921 gaggtaagct ttgtagagag gcaaaaatct gataagacac tcgagaagca ggctagagtg
    13981 ttatcgattc cgattgcctc tccattgtat tttatggtcc aaaggcaagg tgaaactcat
    14041 cgagaagaag gcaatgagga aaatcgtcaa cgtttaatgc aagaaattaa gtcagggaag
    14101 gtaaggaaag tattggtaaa atatgacgtt cttttgactg aaaaccagag cttagaaaat
    14161 attctgcaac gattgttaga aggctttgag aaagtgttac cttactatca agtttgtcaa
    14221 actattagct aagttcatag aaaaaatgag ttggtatacc aactcatttt agagtgccta
    14281 atttggttta ataatatcga tattacccat atatggacga agaacttctg ggatagtcac
    14341 agaaccatct tcattttgat aattttcgag gattgctgct acagtacgtc caactgcaag
    14401 tcctgaacca tttaaagtat gaagcaacct aactttgcca tcaacttcgt cacggtaacg
    14461 aatttgagca cgacgtgctt ggaaatcttc agtattagaa catgaagaaa tttctcgata
    14521 agtattttga gcgggaatcc atacctctaa gtcatacgtt ttagctgctg agaatcccat
    14581 atctcctgta cacaaggtaa tgacacggta tgggagatta agtttttgaa gaatattttc
    14641 agcatttgct gtcatttttt ctaattcttg gtacgattcc tcaggtttgg caaatttcac
    14701 catttctact ttatggaatt gatgaaggcg aattaatcca cgtgtgtcac gtcctgcaga
    14761 gcctgcttct gaacggaatg atggactcat ggcagtaaag tatataggta attctttccc
    14821 atcgataatt tcatcacgat aatagtttgt taaaggaact tcagcagttg ggataagtac
    14881 gaatgggcta tctgctaatt cgaaagtatc ttccttgaac tttggatatt gtcccgtacc
    14941 gaacattgaa tcgtggttaa ccatataagg agggatcact tcagtgtacc cttccttagc
    15001 gtgttcatcc agcatgaagc tgtagatagc acgctctaaa cgagcaccta gacctttgta
    15061 gaagaggaag cgagaaccag taacttttgc accacgttcc caatcgagaa ttccaaggct
    15121 ttcacctaaa tcccagtgag ctttggtctc aaaatcaaat tcacgaggag ttccccagcg
    15181 acgaacttca acattttcat cctcgtctgc tccaagcggc acatcatcag ctggtgtatt
    15241 aggaagtacg gtcaccattg attgtaaatt agcatccaca tctgccaatt ccgcatcaat
    15301 tgctttgata tcagcggaaa gtgtttgcat agctgcgatt tgctcgtctg cattctcttt
    15361 atttcgtttt gcttgagcaa tggcagcaga agcaacatta cgttgcgctt tcgcctcttc
    15421 agctttgata agtagttcac ggcgttttat atcaagttct ttaagggttg ttaatgtttc
    15481 ttggtcaaca ccacgcatgg ccaatttttt agcgacagca tcaaaatctg tacgaatacg
    15541 ttttaagtct aacatatagg aatctccttt atactaaaaa aaacaccctg tcatgctgga
    15601 gcgcaaaggc gcggttccat ccagcttcac agaagtgcac ttgattgatt gattaaatta
    15661 tttcccataa atggaacacg gtagaatttc ataaaacaat cttatctgct cgcaacgacc
    15721 gcagactctc tgaaaagaac tattatacta cttatccgtt aaggttatta tatccaaaaa
    15781 taagtaaaat gtaaacctta actttataag cctgctcttt gttttagtga aatagctatg
    15841 ctaaaatatg attatggaaa agtatacaag tattaaacaa tcaggtcgtc attacggctt
    15901 agacctttta aggattattt ccatgttcat gattgttatt acgcatattc ttggaaaagg
    15961 tggtctgaga tcttcggttg agggacacgc agactcctat tttatagtaa cgtggattat
    16021 ccaagtctta gtttatgggg ccgttaactg ttacgcacta ataagtggtt atgtgggaat
    16081 taattcgcgt tatcgttact caaaattact gtctatctgg gcacaggttt ttttctacac
    16141 ctttacaata acggcacttt ttgaaatcac aggtcatgaa gtgaccttgc ttaattggag
    16201 agatgctttt ttccctattg tcagtgggca gtattggtat attactgctt actttggtct
    16261 attggttttt atgcctgtta ttaataatgg attaaatgcc ttgactgata aacaattaaa
    16321 acaattggtt ttgttaatgt ttattatttt ttcaatttta ccagctgttt taaacaatcg
    16381 tgttccagaa ttttcattga gcaagggttt tgaaatgact tggctactga ttttatatat
    16441 tattggtgcc tatttgaaac gtattgattt aaatatattt aaaacgtcat acttacttat
    16501 agtttatcta ttatcattgg ttgctactta tgtcatgaag tttagtgttg gtgatatttg
    16561 gtattggtat gtttcgccta ctttaacttt aggggcggta agtttgttta tattatttgc
    16621 tagagcaagc attaagccct cgggattctt aaagaaaata attgttgtat tagccccttc
    16681 aaccttggga gtttatctct gtcatctcca cccactaata gtaaaatatt ttgtaagaga
    16741 ttttgcagag acgtttgttt atgagtccat ttatctttac ccatttttaa ttttaggagc
    16801 agggatttta atttatttgt tggcttttat tgtagaacgt attagattac gaatatttaa
    16861 agcaatccat ttttctaact ttttgagatg g
//
LOCUS       DBJYSH010000040        16756 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.040, whole genome shotgun sequence.
ACCESSION   DBJYSH010000040 DBJYSH010000000
VERSION     DBJYSH010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 16756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16756
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.040"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            174..620
                     /locus_tag="ACUBV3_001005"
                     /pseudo
     CDS             174..620
                     /locus_tag="ACUBV3_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631803.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
     gene            781..1683
                     /locus_tag="ACUBV3_001006"
     CDS             781..1683
                     /locus_tag="ACUBV3_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000492950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HHU3847513.1"
                     /translation="MFSSDFGFEIINVDDLSLQEERFLPSELLAYARDENESSFVRDI
                     EGHLTLVYQLLDTQGHVDDVRHVPRVIPVTLFLKEDGLFVLANHKNINLVKKALNRVE
                     KVDSPKHLLLSLVTAFSKQYFDVLDTISEERDKLINDLRKRPNKSNLARLANLQSGTV
                     HLMMGTKQNFEMLTDLQNIEQDKENTRNEKMQLQDAIIEARQLSNMCSLNSQVFQELS
                     SSYNNVLSNNLNDNVTTLTIISIGISIIAMVTSFYGMNVKLPFDSVDAVWVLIILITT
                     IITIMLSIVMYIYVHKEDNHPKHK"
     gene            1843..2907
                     /locus_tag="ACUBV3_001007"
     CDS             1843..2907
                     /locus_tag="ACUBV3_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HHU3847514.1"
                     /translation="MSIESDFFRKKRFIFSSLEEFGFIKSDQEYIYCQTFMDNDFKAI
                     ITISLDGKIAGKVIDSALEEEYLPLRAANYNGSFVGEVRSAYMAILGDISDSCCKDLL
                     FTKDQSNRLAEKIAKTFEDSVDYPFAKHPQYASYRVSGKWYALLFPLKMGKLENVPAQ
                     LSEDEVEVLNIKVNPQDMEILLQKEGIYPSYHMSKKTWVSIVLDNTLSDIEIFKLVSD
                     SRKLVSPNKKSNSEPEFWIIPANPKFYDIDKEFAEHQLIDWTQKGKIAVGDYVFIYIT
                     APIRALRYVCQVIERDLKTSTYLGARDVKAYMRLKLIRQFEDSTYPVAFLAEHGVKTV
                     RGPRRMTKELRQVMAKTILE"
     gene            3020..4012
                     /gene="asnA"
                     /locus_tag="ACUBV3_001008"
     CDS             3020..4012
                     /gene="asnA"
                     /locus_tag="ACUBV3_001008"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HHU3847515.1"
                     /translation="MKKSFIHQQQEISFVKNTFTQYLIDKLEIVEVQGPILSQVGDGM
                     QDNLSGIEHPVSVKVLNIPEAEFEVVHSLAKWKRHTLARFGFNEGEGLFVHMKALRPD
                     EDSLDPTHSVYVDQWDWEKVIPDGRRNLDYLKETVEKIYKAIRLTELAVEARFDIESI
                     LPKRITFIHTEELVEKYPDLSPKERENAIAKEYGAVFLIGIGGELADGKPHDGRAPDY
                     DDWTTPSENGFKGLNGDILVWNEQLGTAFELSSMGIRVDEDALKRQVVLTGDEDRLEF
                     EWHKTLLRGFFPLTIGGGIGQSRLAMFLLRKKHIGEVQSSVWPKEVRDTFENIL"
     gene            complement(4057..4506)
                     /locus_tag="ACUBV3_001009"
     CDS             complement(4057..4506)
                     /locus_tag="ACUBV3_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001893380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductase thioredoxin"
                     /protein_id="HHU3847516.1"
                     /translation="MIKKLLLSLIAILCLTTLKSNLSSADTMTISPQQYQHMITNSDL
                     IAITDKALVSKSQQSEATIFIGRPTCQYCRAFLPKLLKSQATLHSKIYYLDSQKYKGE
                     RLKSFFKKHHITTVPNLAHYQRGKMTKYLVQGSQATPQQIQTFLSQK"
     gene            4637..5176
                     /gene="rsmD"
                     /locus_tag="ACUBV3_001010"
     CDS             4637..5176
                     /gene="rsmD"
                     /locus_tag="ACUBV3_001010"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HHU3847517.1"
                     /translation="MRVVAGTFGGRPLKTLDGKTTRPTTDKVKGAIFNMIGPFFEGGR
                     VLDLFSGSGSLAIEAISRGMDQAVLVEKDRRAQVVIQENIAMTKSPEQFQLLKMEANR
                     ALEQLTGQFDLVLLDPPYAKEEIVKQIQIMDSKGLLGDDIMIVCETDKSVDLPEEIAS
                     FGIWKQKIYGISKVTVYVR"
     gene            5188..5478
                     /locus_tag="ACUBV3_001011"
     CDS             5188..5478
                     /locus_tag="ACUBV3_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000384890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3847518.1"
                     /translation="MDYKLVMIILILVILIYLLLRLHYLEELLKHYHFKNDEGVCSLS
                     RTKNPLYLLMAMITFLPNLINWIANGFEAIDIVRLIPVVVFLWLYYCGDDDE"
     gene            5475..5960
                     /gene="coaD"
                     /locus_tag="ACUBV3_001012"
     CDS             5475..5960
                     /gene="coaD"
                     /locus_tag="ACUBV3_001012"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HHU3847519.1"
                     /translation="MTKKALFTGSFDPVTNGHLDIIERASYLFDHVYIGLFYNLEKQG
                     YFSIECRKKMLEEAIRQFKNVSVLVAQDRLAVDLAREVGAKYFVRGLRNSQDFDYEAN
                     LEFFNKQLADDIETVYLSTSPSLSPISSSRIRELIHFKASVKPFVPKSVVREVEKMSE
                     E"
     gene            5950..7023
                     /locus_tag="ACUBV3_001013"
     CDS             5950..7023
                     /locus_tag="ACUBV3_001013"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepM family pheromone-processing serine
                     protease"
                     /protein_id="HHU3847520.1"
                     /translation="MKNKDPKRKHKSLLGLLKWWIIGFAFLLLVLASLVVRLPYYLEM
                     PGGAYDIRSVLKVNKKADKAKGSYNFVAVSVSQATPAQVFYAWLTPFTELSSKEETTG
                     GFSNDDYLRINQFYMETSQNESVYQALKLANKQVSLTYKGVYVLNLAKNSTFKDRLHL
                     ADTVTGVNGKSFKNSSQLIKYVAALHLGDKVKVQYTSQGKKKESVGKVIKLSNGKNGI
                     GIGLTDHTEVSSDVPVDFNTEGVGGPSAGLMFTLAIYDQLVKEDLRKGRKIAGTGTIE
                     QNGHVGDIGGAGLKVVSAAKKGMDIFFVPNNPIDKNAKKGKTKVQTNYQEAKAAAKRL
                     GTKMKIVPVQNVQQAIDYLKKTK"
     gene            7098..8432
                     /locus_tag="ACUBV3_001014"
     CDS             7098..8432
                     /locus_tag="ACUBV3_001014"
                     /EC_number="3.1.-.-"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HHU3847521.1"
                     /translation="MTELIRILHLNDLHSHFENFPKVKRFFHDNQAQPIETISLDLGD
                     NIDKSHPLTEASSGKANIQLMNELGIELATIGNNEGVGLSKKDLDQVYKDSDFTVIVG
                     NLKDNTIEPSWAKPYIIYETQQGTKLAFLAYTFPYYKTYEPNGWMIEDPIDCLKCHLQ
                     INEIKEANCRILMSHLGIRFDTRIAQEFSEIDLIIGAHTHHLFEEGELINGTYLAAAG
                     KYGRFVGSIDITFDNHTLKDILISTCDTKQLTGYPSDSDWAKKIESEGKEQLRKESLI
                     SFPAPLSLEESCQLVMDAMLFYAGADVAIINSGLIVQPFRKDFSRKNLHESLPHQMRL
                     AKLTVSSQELLEIYETIYQQGQFLAQQKIHGMGFRGKYFGDVLHSGFDYKNGKIVYNE
                     KDIDAKEEVILVIVDQYYFASYFECLKTKKVDLLFPELLRDVVADYLINMTL"
     gene            8502..9080
                     /locus_tag="ACUBV3_001015"
     CDS             8502..9080
                     /locus_tag="ACUBV3_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HHU3847522.1"
                     /translation="MRKEVTPEMLNYNKYPGPQFIHFENIVKSDDIEFQLVINEKSAF
                     DVTVFGQRFSEILLKYDFIVGDWGNEQLRLRGFYKDASTIRKNSRISRLEDYIKEYCN
                     FGCAYFVLENPNPRDIKFDDERPHKRRKSRSKSQSSKSQTRNNRSQSNANAHFTSKKR
                     KETKRRQERHIKEEQDKEMTSAKQHFVIRKKK"
     gene            9131..10237
                     /gene="rlmN"
                     /locus_tag="ACUBV3_001016"
     CDS             9131..10237
                     /gene="rlmN"
                     /locus_tag="ACUBV3_001016"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001124995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HHU3847523.1"
                     /translation="MPKKTDTPDYKPSIYSLTRDELIAWAIEHGEKKFRASQIWDWLY
                     KKRVQSFDEMTNISKDFIALLNENFVVNPLKQRIVQESADGTVKYLFELPDGMLIETV
                     LMRQHYGLSVCVTTQVGCNIGCTFCASGLIKKQRDLNNGEITAQIMLVQKYFDERGQG
                     ERVSHIVVMGIGEPFDNYTNVLKFLRTVNDDNGLAIGARHITVSTSGLAHKIREFANE
                     GVQVNLAVSLHAPNNDLRSSIMRINRSFPLEKLFAAIEYYIETTNRRVTFEYIMLNGV
                     NDTPENAQELADLTKKIRKLSYVNLIPYNPVSEHDQYSRSPKERVEAFYDVLKKNGVN
                     CVVRQEHGTDIDAACGQLRSNTMKRDRQKAKVGQ"
     gene            10237..10752
                     /locus_tag="ACUBV3_001017"
     CDS             10237..10752
                     /locus_tag="ACUBV3_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HHU3847524.1"
                     /translation="MLKRLLTEDGELTKISRRFVWMLVIIYCLIIVRMCFGPQIMIEG
                     VSTPNVQRFGRIVALLVPFNSFRSLDQLTSFKEIFWVIGQNVVNILLLFPLIIGLLSL
                     KPSLRKYKSVILLAFLMSLFIECTQVVLDILIDANRVFEIDDLWTNTLGGPFALWTYR
                     NIKGWLLTIRK"
     gene            10948..12693
                     /locus_tag="ACUBV3_001018"
     CDS             10948..12693
                     /locus_tag="ACUBV3_001018"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003063634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HHU3847525.1"
                     /translation="MSIIKNLWWFFKEEKKRYLIGILSLSLVAVLNLIPPKIMGLVID
                     AITTGKLTRPQLLWNLLGLVLSALAMYGLRYIWRMYILGTSYKLGQVVRYRLFEHFTK
                     MSPSFYQKYRTGDLMAHATNDINSLTRLAGGGVMSAVDASITALVTLITMFFTISWQM
                     TLIAVIPLPLMALATSKLGRKTHETFKESQAAFSELNNKVQESVSGVKVTKSFGYQEQ
                     EIASFQEVNQMTFVKNMRTMTYDVMFDPLVLLFIGASYVLTLAMGAFMISKGQVTVGD
                     LVTFVTYLDMLVWPLMAIGFLFNMVQRGSVSYNRINSLLEQESDITDPLNPIKPVVNG
                     TLRYDIDFFRYDNEETLADIHFTLEKGQTLGLVGQTGSGKTSLIKLLLREHDVTQGKI
                     TLNKHDIRDYRLSELRQLIGYVPQDQFLFATSILENVRFGNPTLSINAVKKATKLAHV
                     YDDIEQMPAGFETLIGEKGVSLSGGQKQRIAMSRAMILDPDILILDDSLSAVDAKTEH
                     AIIENLKTNRQGKSTIISAHRLSAVVHADLILVMQDGRVIERGQHQELLNKGGWYAET
                     YASQQLEMEEAFDEV"
     gene            12683..14422
                     /locus_tag="ACUBV3_001019"
     CDS             12683..14422
                     /locus_tag="ACUBV3_001019"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HHU3847526.1"
                     /translation="MKSNQWQVFKRLISYLRPYKWFTVLALSLLLLTTVVKNIIPLIA
                     SHFIDHYLTNVNQTAVLILVGYYSMYVLQTLIQYFGNLFFARVSYSIVRDIRRDAFAN
                     MERLGMSYFDRTPAGSIVSRITNDTEAISDMFSGILSSFISAIFIFTVTLYTMLMLDI
                     KLTGLVALLLPVIFILVNVYRKKSVTVIAKTRSLLSDINSKLSESIEGIRIVQAFGQE
                     ERLKTEFEEINKEHVVYANRSMSLDSLFLRPAMSLLKLLAYAVLMSYFGFTGVKGGLT
                     AGLMYAFIQYVNRLFDPLIELTQNFSTLQTSMVSAGRVFDLIDETGFEPSQKNTEAFV
                     REGNIEFKNVSFSYDGKKQILDNVSFSVKKGETIAFVGATGSGKSSIINVFMRFYEFQ
                     SGQVLLDGKDIRDYSQEQLRKNIGLVLQDPFLYHGTIKSNIKMYQDITDQEVQDAAEF
                     VDADQFIQKLPDKYDSAVSERGSSFSTGQRQLLAFARTVASKPKILILDEATANIDSE
                     TEQIVQDSLAKMRQGRTTIAIAHRLSTIQDANCIYVLDRGKIIESGNHESLLDQKGTY
                     YRMYQLQAGMMEV"
     gene            14550..15116
                     /locus_tag="ACUBV3_001020"
     CDS             14550..15116
                     /locus_tag="ACUBV3_001020"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HHU3847527.1"
                     /translation="MLLLVDNYDSFTYNLKQYLSVYKEIFVIKNDDPNLFLLAESAEA
                     IVLSPGPGHPKDAGKILELINQFIGQKPILGICLGHQALAECLGGRLNLANHVMHGKQ
                     SWVTINDHTSLFKGIDSPTQVMRYHSLVVTDLPENIAIIARSNEDNEIMAFHCPSLKV
                     YAMQFHPESIGSIDGMKMIENFLTLIND"
     gene            complement(15183..15722)
                     /locus_tag="ACUBV3_001021"
     CDS             complement(15183..15722)
                     /locus_tag="ACUBV3_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HHU3847528.1"
                     /translation="MNTRTTTYIALMVALLIVLGFIPGIPLGFIPVPIVLQNLGVMLA
                     GALLGSRKGFLAVAIFLLLVAIGAPFLPGGRSGLVTLFGPTAGYLLTYPFAAFFIGLG
                     LEKVKTTKLWVQFLIIWIFGVLLIDICGSIVLSFQTSLPLTKSLFSNLIFIPGDTLKA
                     SICLIIYRKFANRLTHLYN"
     gene            complement(15723..16715)
                     /gene="bioB"
                     /locus_tag="ACUBV3_001022"
     CDS             complement(15723..16715)
                     /gene="bioB"
                     /locus_tag="ACUBV3_001022"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HHU3847529.1"
                     /translation="MSFQTNYIHLADEILSGKTSISYEQALEILNSDENWWEIYAAAL
                     YLKNQVSRNNIRLNVLLSAKQGLCAENCGYCSQSKESTADIDKFGLLPQNVILKQAIV
                     AHQNGASVFCIAMSGTKPSKREIEQLCQVIPEIKKSLPLEICLTAGFLDREQLHQLKQ
                     AGIDRINHNLNTPEENYPNIATTHSFKDRCDTLERIHNEDIDVCSGFICGMGESDEGL
                     ITLAFRLKELNPYSIPVNFLLAVEGTPLGKYNYLTPIKCLKIMAMLRFVFPFKELRLS
                     AGREVHFENFESLVTLLVDSTFLGNYLTEDGRNQHTDIEFLEKLQLNHTKKELI"
BASE COUNT         5370 a         2578 c         3346 g         5462 t
ORIGIN      
        1 ctttgcgggt gtgtagtttc aactatttta aatctttcaa attatgctat taaaattgtc
       61 taacttttaa cagagatgcg attaaattgt attaatccct aaaaagataa attgctagtt
      121 cttatgctat aataactcta tcaatgtcaa aggagttttt atgtcaaagg tgaggaacag
      181 tgcattttat ggcgccctac gccctgtctt atttacaata tctatttgat gcgactataa
      241 ctcatgctag tgggactgcg acttttccaa aaggtcaaag cgatagtcaa tccaaactac
      301 tcttgcaatt atcaaatggg gtcctcgtag atatcttctt gacaacacgt ttaaatttac
      361 cgcatgaaat gattatttat ggaactgaag gtcgtctaat cattccacat ttttggaaaa
      421 ccactcatgc aaaattggtg agaaatgata ctagtgcgca aacgattcaa gttgacatgg
      481 ttagtgattt tgagaaagaa gcttatcatg ttagtcaaat gattttagaa gggcaaaggg
      541 taagtcatat catgacgcct cagttgacgc tgtcaggtgt taagattata gaagacttat
      601 accgttcatg ggggaaatga tgctggctat ctagcatatt aataaggagt actagatgtt
      661 attttagtag tccttttttg ttttgctctc ataaggttaa ttagatttaa aagggttttc
      721 tacttgtttt ttctcgattt ttgtttcata atgtagacaa gtttagagtg aggtgcttct
      781 atgttttcga gcgattttgg atttgaaata attaatgttg acgacctatc cttgcaagag
      841 gagagatttt taccttcaga attgctggct tatgcacgtg atgaaaatga gtcttctttc
      901 gtacgtgata ttgaggggca tttgacactt gtttaccagc ttttggatac gcaaggtcac
      961 gttgatgatg ttcgtcatgt gccgagagtg ataccagtga ctctattttt gaaagaagat
     1021 ggcttgtttg tattagctaa tcataaaaat attaaccttg taaaaaaagc acttaatcgt
     1081 gttgaaaagg tagattctcc gaaacacctc ttattatcat tagtgactgc tttctcaaag
     1141 caatattttg atgttttaga tactatttct gaagaaagag ataaactaat caatgatttg
     1201 cgtaaaagac caaacaaatc gaacttggca aggctggcaa atttacaaag tggaacagta
     1261 cacttaatga tgggaactaa gcaaaatttt gaaatgctga ccgatttaca aaacattgag
     1321 caagataaag agaacactag aaatgaaaag atgcaacttc aagatgctat tattgaagca
     1381 agacaattat ctaacatgtg ttccttaaat tcacaggttt tccaagaact ttcttcttct
     1441 tataataatg tcctcagcaa taaccttaac gacaatgtaa ctaccttaac aattatttcc
     1501 attggtattt ccattattgc tatggttacc agcttttatg gaatgaatgt taagttgcct
     1561 tttgacagtg ttgatgctgt ctgggtattg attattttaa ttacgactat cattactatt
     1621 atgctctcta ttgttatgta catttatgtc cataaagagg ataatcatcc caaacataaa
     1681 tagagaatta ataagagggt gctatcaatt agtttagttt taagcatggc aacatgcttt
     1741 ttttgtacaa agaaactaga attcatcttt gactttgagc ttgctttcta gctcttaaaa
     1801 cgacataata gaggataatg tacagtgaat agagaggtta ctatgtcaat agaatcagat
     1861 tttttccgga aaaaacgttt tattttttca tcgcttgagg agtttggatt tatcaaatct
     1921 gaccaagagt atatctattg tcaaacattt atggataatg actttaaagc tattatcacg
     1981 atttcattgg atggaaagat agctggtaag gttattgatt cggcacttga agaagaatat
     2041 ttaccgcttc gagcagcaaa ttataatggt tcgtttgttg gcgaagtacg atcggcttat
     2101 atggctatat taggtgatat ttcagattct tgttgtaagg acttattatt taccaaagat
     2161 caaagtaatc gtttagcgga gaagatagct aaaacttttg aagattcagt tgattatcca
     2221 tttgcgaagc acccacaata tgcttcttat cgtgtatcag gtaaatggta tgctctgctt
     2281 tttccattga agatggggaa actagaaaat gttccagctc aactgtcaga agatgaggtt
     2341 gaagttttga atattaaagt caatcctcaa gatatggaaa tactcttaca aaaagaaggt
     2401 atttatcctt cttatcatat gtctaaaaag acttgggtat ctatcgtttt agacaatact
     2461 ttgtctgata tagaaatttt taaattggta tcagatagtc gtaagcttgt ttctcccaat
     2521 aaaaaatcaa attctgagcc agagttttgg attattcctg ctaatcctaa attttacgat
     2581 attgataagg agtttgcaga gcatcaattg attgattgga ctcaaaaagg aaagattgcg
     2641 gtgggagatt atgtgtttat ttatattaca gcacctattc gtgctttgcg ctatgtctgt
     2701 caagtaatag aacgtgacct taaaacaagt acttacttag gagcaagaga tgtcaaagct
     2761 tatatgcgac tcaaactgat aaggcaattt gaagattcta cctatcctgt agcattttta
     2821 gcagaacacg gcgttaagac tgtgagagga ccacggcgaa tgaccaagga attgcgacaa
     2881 gtcatggcaa aaacaattct agagtaaaga ttttcagaaa aacttgtaaa aaagtcagaa
     2941 taattctaca actcctcttc cattttgtta cttgaaggag tataatagct ctaacatttt
     3001 ataatttaag gaggaacata tgaagaaaag ctttattcat cagcaacagg agatttcttt
     3061 tgtcaaaaat acttttacac aatatttgat tgataagtta gaaatcgtcg aagtgcaagg
     3121 acctattctt agtcaggtcg gggatggtat gcaagataat ttatctggta ttgaacatcc
     3181 tgtatcggtg aaagtcctta atatacctga agctgaattt gaagtcgttc actcattggc
     3241 taaatggaaa cgtcatactt tggcaagatt tgggtttaat gagggagaag gtcttttcgt
     3301 tcatatgaaa gctcttcgtc cagatgaaga ttctcttgat ccaacacact cagtttatgt
     3361 agatcagtgg gattgggaga aggtcattcc tgatggtaga cgtaatttgg actacctaaa
     3421 agaaactgtg gaaaaaatct acaaagctat ccgcctaaca gagcttgccg tggaagctcg
     3481 ttttgatatt gaatccattt taccaaagcg tattaccttt attcatacag aagaattggt
     3541 agaaaaatac ccagacttaa gccctaaaga gcgtgaaaac gccatcgcta aggagtatgg
     3601 cgcagtcttt ttgattggta ttggtggaga gttagcagat ggtaaaccac atgatggtcg
     3661 tgcaccggac tatgatgact ggacaacacc atcagagaat ggttttaaag gtctcaatgg
     3721 tgatatttta gtatggaacg aacagcttgg aactgccttt gaactatcat caatgggaat
     3781 tcgagttgat gaagatgctc ttaaacgtca agttgttctc accggtgatg aagatcgttt
     3841 agagtttgag tggcataaga cgctattacg tggttttttc cctttgacta ttggtggggg
     3901 aattggtcaa tcacgattag ctatgttctt gcttcgtaaa aaacatattg gagaagtaca
     3961 gtcaagtgtc tggccaaaag aagtcagaga taccttcgaa aatatattat aaaaaatcag
     4021 ccaagcagtt ttgtttggct gattttaaca ataagtttat ttttggctta agaaagtttg
     4081 gatttgttga ggtgtagctt ggcttccttg aacaagatat ttagtcattt ttcctcgttg
     4141 ataatgtgct aaatttggaa cagtggtaat atgatgcttt ttgaaaaaac tcttcaatcg
     4201 ttcccctttg tatttttgac tatcaaggta gtaaattttt gaatgaagtg tggcttgaga
     4261 ttttaataat tttggtaaga aagctctaca atattggcaa gttggacgtc ctataaaaat
     4321 agtcgcttca gattgttgac ttttgcttac taacgcctta tctgtgatgg cgattaagtc
     4381 actatttgtt atcatatgtt ggtattgttg tggagaaata gtcatggtgt cagcgctact
     4441 taagtttgat tttagcgttg taaggcataa tatggctatt aaacttaata ataatttttt
     4501 aatcattatg ggaacctcgt ttctcttact gctagcttat catatcaaaa aagaaggaaa
     4561 agagatacca tcttctctgt ttgttataag caattttttt gataactcta ccttattttt
     4621 tgttataata gtggttatga gagtagtagc aggaacattt ggagggcgtc ctttaaaaac
     4681 gcttgatgga aaaacaactc gtccaacaac tgataaggtt aaaggcgcta tttttaatat
     4741 gattggtcct ttttttgaag gtggtcgtgt tttagacctt ttttctggca gtggtagctt
     4801 agctattgag gcgatctcaa ggggaatgga ccaagctgtc ctagttgaaa aagataggcg
     4861 tgcgcaggtc gttattcaag aaaatattgc aatgactaag agtccggagc aatttcaatt
     4921 attaaaaatg gaagccaacc gtgctttaga acaattaacg ggacaatttg atttggtctt
     4981 gttggacccg ccatatgcta aggaagaaat tgtaaagcaa atccaaatca tggatagcaa
     5041 gggtttatta ggcgatgata tcatgattgt ttgtgaaacg gacaaatcag ttgatttacc
     5101 tgaagaaatt gcttcttttg ggatatggaa acagaaaatt tatggcattt ctaaagtaac
     5161 ggtctacgtt cgttaggagg ctttggtatg gattacaagt tagtgatgat tattctcatc
     5221 ttagtcatat tgatttattt gttgttgcgc cttcactatt tagaagagtt gctaaaacat
     5281 tatcacttca aaaatgatga aggagtttgt tcattatcaa gaacaaaaaa tccattgtat
     5341 ctattaatgg caatgattac ttttttgcca aatcttatca attggatagc taacggtttt
     5401 gaggcaatag acattgttcg cctgattcct gtggtagtct ttttatggtt gtattattgt
     5461 ggagatgacg acgaatgaca aagaaagctt tatttacagg ttcatttgat cctgttacta
     5521 atggacattt agatattatt gagcgcgcca gctatctatt tgatcatgtt tacattgggt
     5581 tattttataa ccttgagaag caaggttact tttcgattga gtgccgtaaa aaaatgttag
     5641 aagaagctat ccgtcagttt aagaatgttt cagttttagt ggcgcaagat agactagctg
     5701 ttgacttagc aagggaagtg ggagcaaaat attttgttcg aggtctgcgt aatagccagg
     5761 attttgatta tgaagccaat ttggagtttt ttaataaaca gttggcagat gatattgaga
     5821 cagtttattt atcaacgtcg ccatctttga gtccgatatc atctagtcgc atccgtgaat
     5881 tgatacattt taaggcttct gtgaagcctt ttgttccaaa gagtgttgtt agagaagtgg
     5941 agaaaatgag tgaagaataa agatccaaaa agaaaacata aaagtttact aggtctgctt
     6001 aaatggtgga ttataggttt tgctttttta ttgttagtat tagcaagcct tgtggtgaga
     6061 ctaccttatt atttggaaat gccaggtgga gcttatgata tccgttcggt attgaaagtg
     6121 aataaaaagg ctgataaagc taagggttcg tacaattttg tagcggtatc tgttagtcaa
     6181 gcaacgccag ctcaggtttt ttatgcttgg ttaactcctt ttacagaatt atctagtaaa
     6241 gaggagacaa caggtggctt tagtaatgac gattatctca gaattaatca attctacatg
     6301 gagacttctc aaaatgagtc cgtctatcaa gctttaaagt tagctaataa gcaagtatct
     6361 cttacttata aaggagttta tgtgcttaac ttagctaaaa attcgacatt taaagataga
     6421 ttacatttag ctgatacagt cacaggagtt aatggcaaaa gtttcaaaaa ttcttctcaa
     6481 cttatcaaat atgttgcagc tcttcatcta ggtgataaag taaaggtaca atacacaagt
     6541 caaggtaaaa agaaagaatc agttggtaaa gttattaaac tatcaaacgg taaaaatggt
     6601 attggtatcg gtttgacaga ccatacagaa gtatcatctg atgttcctgt ggactttaat
     6661 actgaaggtg ttggaggacc aagtgcaggt ttaatgttta ctttagcgat ttacgaccaa
     6721 ttagttaaag aagatttacg taaaggacgt aagattgcag gcactggtac tattgagcaa
     6781 aatgggcatg ttggcgatat tggtggagca ggtttgaaag ttgtttcagc ggctaaaaaa
     6841 ggaatggata ttttctttgt tcctaataat ccgatcgata aaaatgctaa aaaaggcaaa
     6901 acaaaggttc agacgaatta tcaagaggca aaagcagcag ctaagcgatt aggtacgaag
     6961 atgaaaattg taccagttca aaatgttcag caagctattg attatttgaa aaaaacaaaa
     7021 taaactcaaa gagttcgggc gtctgctcgg cttttttgtt ttgcgacatt tgactatcaa
     7081 agtgctaaaa tgatagtatg acagagctta taagaatact acatttaaat gatctgcact
     7141 ctcattttga gaattttcct aaggtaaaac gattttttca tgataatcaa gcgcagccaa
     7201 tagagacgat ttcacttgac ttgggggata atattgataa aagccatcct ttgacagaag
     7261 caagctcggg caaggctaat atccaattga tgaatgaact gggtattgag cttgctacta
     7321 ttggtaataa tgaaggagtt ggtttatcca aaaaagactt ggaccaagtc tacaaagatt
     7381 cagattttac ggttattgtt ggcaatctaa aagataacac aatcgaaccg agttgggcaa
     7441 aaccttacat tatttatgag acacagcaag gaactaagct tgcctttttg gcttatactt
     7501 ttccttatta taaaacctat gaacctaatg ggtggatgat agaagatcca attgactgtc
     7561 ttaaatgcca tttacaaata aatgagataa aagaggcaaa ttgtcgtatt ttgatgagtc
     7621 atttaggtat tcgttttgat acaaggattg ctcaagaatt ttcagaaatt gaccttatta
     7681 taggtgcaca tacacaccat ttatttgagg aaggggagct aattaacggt acatatcttg
     7741 cagcagctgg taaatatggt cgctttgttg ggagtattga tataactttt gataatcata
     7801 cactaaaaga tattttaatc tcaacatgtg atacaaaaca actaactggt tatccttcgg
     7861 acagtgactg ggctaagaag attgagtcag aaggtaaaga acagcttaga aaagaaagtt
     7921 taatatcctt tccagcacca ttatcattag aagaatcgtg tcaactagtc atggatgcga
     7981 tgctgtttta tgcaggtgca gatgttgcta ttatcaattc tggcttaatt gttcagccat
     8041 ttagaaaaga cttttcgagg aaaaatctcc acgaatcatt acctcatcaa atgcgtctcg
     8101 ctaaattgac agtgagtagt caagaacttt tagagattta tgaaactatt tatcaacaag
     8161 gtcaattttt agcgcaacaa aagattcatg gtatgggctt tcgtggcaag tactttggag
     8221 acgttttaca ttcgggattt gattacaaaa atggaaaaat agtgtataat gagaaagata
     8281 ttgatgccaa agaagaagtg attcttgtaa tcgttgatca gtattatttt gcatcgtatt
     8341 ttgagtgttt aaaaacgaaa aaagtagact tgctttttcc tgaattactc agagatgtgg
     8401 tagcagatta tttaataaat atgacactat aaatgaaaaa tagataaata aagagtagag
     8461 gctagtcgtt tcttctcaat gattttagag aaaggagtat tatgcgaaaa gaagtgacac
     8521 cagagatgct taactataat aagtatcctg gcccacagtt cattcacttt gaaaatatcg
     8581 ttaaaagtga tgatattgaa tttcaacttg ttattaatga aaaatcagct tttgatgtga
     8641 ctgtctttgg acaacgtttt tctgagattt tattaaaata tgattttatc gttggcgatt
     8701 ggggtaacga gcagttgagg ctaagaggct tttacaaaga tgctagtacg attagaaaaa
     8761 atagccggat ttcacgttta gaagattata ttaaagagta ttgtaacttt ggttgtgctt
     8821 attttgtgtt ggagaatcca aatcctagag atattaaatt tgatgatgaa agacctcata
     8881 agcgtcgtaa gtcaagatcc aaatcacaat catcaaagtc acaaactaga aataatcgtt
     8941 cccagtcaaa tgccaatgct cattttacaa gtaaaaagcg taaagagaca aaacgccgtc
     9001 aagaacgtca tattaaagaa gagcaagata aggaaatgac ctctgcaaag cagcattttg
     9061 ttattcgtaa gaaaaaataa gaaagggata aactgtaacc gtttattcca ccatgaagaa
     9121 agaaaatatt atgcctaaaa aaactgatac acccgactat aaaccctcta tttatagctt
     9181 aactcgcgat gaactaatag cttgggctat tgaacatgga gaaaagaaat tccgtgcttc
     9241 acaaatctgg gactggctct ataaaaagcg tgtgcaaagc ttcgatgaaa tgactaacat
     9301 ttccaaagat tttattgcac ttttgaatga gaattttgtt gttaatcctc taaaacaacg
     9361 tattgttcaa gaatctgctg atggaactgt taaatacctt tttgaattac ctgatggcat
     9421 gctgattgag acggttttga tgcgtcaaca ttatggttta tcagtatgtg taacgactca
     9481 agttggttgt aacattggtt gcactttctg tgcaagtggt ttgataaaga aacaacgtga
     9541 tcttaacaat ggcgaaatca ctgctcaaat tatgcttgtt caaaaatatt ttgatgaacg
     9601 tggacaaggt gagcgcgtta gtcacattgt ggtcatggga ataggtgaac cctttgataa
     9661 ctataccaat gtattaaaat tcttgcgtac agttaacgat gataatggat tagctattgg
     9721 tgcacgtcac attacagtgt ctacttcagg tttagcacat aaaattcgtg aatttgcaaa
     9781 tgaaggtgta caagttaact tagcagtatc tcttcatgct ccaaataatg atttacgatc
     9841 aagtatcatg cgtattaatc gttctttccc tcttgaaaag ttgtttgcgg ctattgaata
     9901 ttatattgag acgactaacc gtcgtgtaac atttgagtat attatgttaa atggtgtcaa
     9961 cgatacccca gaaaatgctc aagagttagc agatttaacg aaaaaaattc gcaaattatc
    10021 ttatgtcaat ttaattcctt acaatcctgt ctcagaacat gaccagtata gtcgtagtcc
    10081 taaagagcgt gtagaagctt tctatgacgt tttgaagaaa aatggagtta attgtgttgt
    10141 tcgtcaagaa catggtacgg acattgatgc agcttgcgga caattacgtt caaacacaat
    10201 gaaacgcgat cgtcaaaaag cgaaggtagg gcagtaatgc ttaaaagatt acttactgaa
    10261 gatggggaat tgacaaagat tagtcgtcgt ttcgtttgga tgttagtgat tatctattgt
    10321 cttattattg tcaggatgtg ttttgggcct caaattatga ttgagggggt atcaactccg
    10381 aatgttcagc gcttcggaag aattgtagct cttttagtac catttaattc ttttcgtagt
    10441 ttagatcagc taactagctt taaagagatt ttttgggtta ttggtcaaaa tgtagtgaat
    10501 attttactgc tgtttcctct cattataggg ttactatccc taaagccaag tttacggaaa
    10561 tataaaagcg ttatattact tgctttcttg atgtctcttt tcatagagtg tactcaagtt
    10621 gttttagata ttttaataga tgctaatcgg gtttttgaaa tcgacgatct atggacaaat
    10681 accttaggcg gtcctttcgc cctatggact tatcgaaaca taaaaggttg gcttctaact
    10741 attagaaaat gaaaataatg taaaggctat gacattgttc aagcctttta ctatttaaaa
    10801 agaatcactt tagttgacga aagtaagctt atagaggcat aatagagagt tgttaaatga
    10861 gaattttaaa acacttttca tgtcttctga ttgatttggt cttctgactt aattatgatg
    10921 cttttcaatc tttgagaaag gaaaaatatg tctattataa aaaatttgtg gtggtttttt
    10981 aaggaagaaa agaagcgcta tcttattggg attttgtcat taagtttggt tgcggtgtta
    11041 aatcttatcc ctcctaaaat tatgggatta gttattgatg ctattacaac tggaaaatta
    11101 acaagaccac aattactatg gaatttatta ggtttggttt tgtcagcttt agctatgtat
    11161 gggctgcgtt atatttggcg tatgtatatt ttagggactt cttacaaatt gggccaagtt
    11221 gtcagatacc gtttatttga acattttaca aaaatgtctc cttcttttta tcagaaatat
    11281 cgtacaggtg atttaatggc gcacgcgacc aacgacatca attctctaac acgtcttgca
    11341 ggaggaggag ttatgtcagc agtggatgcc tctatcacag cattagtaac gcttatcacc
    11401 atgttcttta ctatttcgtg gcaaatgaca ttaattgcgg ttatcccttt gcccttaatg
    11461 gccttagcaa ctagtaaatt ggggcgaaaa acccatgaaa ccttcaaaga atctcaggca
    11521 gccttttcag aattaaataa taaagtgcaa gaaagtgtct ctggcgtcaa agtgactaaa
    11581 tcatttggtt atcaagaaca agaaattgct tcttttcaag aggttaatca gatgactttc
    11641 gtgaagaaca tgcggaccat gacttatgat gtcatgtttg atcctttagt tcttcttttt
    11701 ataggtgcct cctacgtatt aacattggct atgggagctt ttatgatttc aaaaggtcaa
    11761 gttactgttg gtgacttggt aacatttgtg acgtatttag atatgttggt atggcccttg
    11821 atggcgattg gtttcttgtt caatatggta cagcgtggta gtgtttctta taaccgtatt
    11881 aatagtctac ttgagcaaga atcggatata actgatcctt taaatcctat caaacctgtt
    11941 gtcaatggaa cattaagata tgatattgat ttctttagat acgacaatga agaaacctta
    12001 gccgatattc atttcacctt agaaaaaggt caaaccttag gtttggtagg tcaaacggga
    12061 tcagggaaga caagtcttat taagttattg ctacgtgaac atgatgtgac tcaggggaaa
    12121 attactttaa ataaacatga tatacgtgat tatcgattgt ctgagttacg tcaactaatc
    12181 ggttatgttc ctcaagatca gtttttattt gctaccagta ttttagaaaa tgttcgcttt
    12241 ggaaatccaa ctctatctat caatgctgtt aaaaaagcaa ctaaattggc tcatgtttac
    12301 gatgacattg aacagatgcc agcaggattt gagactctaa ttggagaaaa aggagtctca
    12361 ttatctggtg gacaaaaaca aaggattgcg atgagtcgtg ccatgatttt agatccagat
    12421 attcttattt tggatgattc tctatcagca gtggacgcta aaacggaaca tgctatcatt
    12481 gagaacctta aaacgaatcg tcaagggaaa tcgactatta tttcagcaca tcgtttatca
    12541 gctgttgtgc acgcagacct tatcttagtt atgcaagacg gcagagtcat tgagcgaggt
    12601 caacatcaag agttgctaaa taaaggtggt tggtatgctg aaacgtatgc ctcacagcaa
    12661 ttagaaatgg aggaagcatt tgatgaagtc taatcaatgg caagtcttta agagattaat
    12721 ctcctattta cgcccttata aatggtttac agtattagct ctatctctct tattgttgac
    12781 gactgttgtt aaaaatatta ttcctttaat tgcttcacat tttattgatc actatctgac
    12841 aaatgttaat caaacagcag ttcttatttt agtgggatat tattcaatgt atgtcttgca
    12901 gaccttaatt caatattttg ggaatctctt ttttgcgcgt gtttcttata gtattgttag
    12961 agatattcgt agagatgctt ttgctaatat ggaaaggcta ggcatgtctt attttgatag
    13021 gacaccggca ggatctattg tgtcacgtat tactaatgat actgaagcaa tatctgatat
    13081 gttttcgggt attttatcaa gttttatctc ggcgatattt atttttacag ttactctgta
    13141 cactatgttg atgctagaca ttaaactaac aggactcgtc gctcttttgt tacctgttat
    13201 ctttatatta gtgaatgtct atcggaaaaa atcagtcact gtcattgcta aaacgagaag
    13261 tttacttagt gatatcaaca gtaaattatc agaaagtatt gaaggaattc gcattgtaca
    13321 ggcttttggt caagaagagc gcttgaagac tgaatttgag gaaattaaca aagagcatgt
    13381 tgtgtatgcc aatcgttcta tgtctcttga tagtctcttc ttaagaccgg cgatgtctct
    13441 tttaaaactc ctagcatatg ctgttcttat gtcttatttt ggatttacag gagttaaagg
    13501 aggtcttacg gcaggattaa tgtatgcttt tattcagtac gttaatcgtc tctttgaccc
    13561 tttaattgaa ctaacgcaaa atttttcaac cttacaaaca tcaatggtat cagcagggcg
    13621 tgtgtttgat ctgattgatg aaacaggttt tgaaccaagc caaaaaaata cagaagcttt
    13681 tgtgagagaa ggaaatatcg aatttaaaaa tgtatcattc tcatatgatg gaaaaaaaca
    13741 aatccttgat aatgtttctt ttagtgttaa aaaaggtgaa actattgctt ttgtaggagc
    13801 gacaggttct ggaaaatcat ctattattaa tgtctttatg aggttttacg aatttcaatc
    13861 tggacaagtt ttattagatg ggaaggatat tagggattac tcacaagaac aattgcgaaa
    13921 gaatattgga ctcgtcttac aagacccctt tttatatcat ggcactatta aatctaatat
    13981 caaaatgtac caagacatca ccgatcaaga ggtacaggac gcagcagagt ttgttgatgc
    14041 tgatcaattt attcagaagt tgccagataa gtacgattca gctgtttcag aacgtgggtc
    14101 aagtttttca actggacaac gccaactatt agcgtttgcc agaactgtgg ctagtaaacc
    14161 taaaatttta atcttagatg aggcgacagc aaatattgat tcggaaacag agcagattgt
    14221 acaagattcg ttagcaaaaa tgcgtcaagg tcgaacaaca attgccattg ctcaccgctt
    14281 atcaaccatt caagatgcta attgtatcta tgttcttgat agaggaaaga taattgaaag
    14341 tgggaatcac gagtctttat tagaccaaaa agggacttat tatcgtatgt accaattaca
    14401 agccggcatg atggaggtct agaaaattta ggctattcaa aaggcataat ctgtgaggct
    14461 tagaggttgg aatagttatt cctctaagcc ttttttatat tttacaatta gtgtataatg
    14521 aggtcaaagt acaaggaaag gtctataaaa tgttactatt ggtagataat tacgattctt
    14581 ttacctataa tttaaagcaa tatttaagtg tctataaaga aatattcgtc ataaaaaatg
    14641 atgatcctaa ccttttttta ttagctgaga gcgcagaagc tatcgtctta tcacctggac
    14701 caggacaccc aaaggatgct gggaaaatat tagaattaat caatcagttt ataggacaga
    14761 aacctattct aggtatctgt ttggggcatc aagcgcttgc tgagtgcttg ggtgggcgat
    14821 taaatttagc aaatcacgtt atgcatggta aacaaagttg ggtaacaata aacgaccata
    14881 ctagtttgtt taagggaatt gatagtccaa cccaagttat gcgttatcac tcattggtag
    14941 tgactgattt accagagaat attgctatta ttgcacgatc aaatgaggat aatgaaatca
    15001 tggcattcca ttgccccagc ttaaaggtat atgcgatgca gtttcatcct gagtcaatcg
    15061 ggagtattga tggaatgaaa atgattgaaa actttttgac gttaattaat gactaaaatg
    15121 tgttagtgta ggataatgac tctctataaa taaaagacag taccttaggt actgtctttt
    15181 atttagttgt atagatgagt taatctattt gcaaatttac gataaataat caagcaaatg
    15241 gatgctttta aggtatcccc aggaataaaa ataaggttag aaaatagtga tttagttaag
    15301 gggagagaag tttgaaagga aagaacgatg ctaccacaaa tatcaatcaa tagtacgcca
    15361 aatatccaaa taataaggaa ttgaacccag agttttgttg ttttcacttt ctcaagccct
    15421 aggccgataa aaaaagctgc aaatggataa gtcagcagat agcctgctgt tggcccaaac
    15481 agcgttacta gacctgaccg ccctcctggt aaaaatggtg caccaatagc aactaagagt
    15541 aagaaaatag ccacagctag gaaacctttc ctactaccaa gcagagcacc tgctagcata
    15601 acaccaaggt tttgtaagac aattgggact ggaatgaaac ctaatgggat ccctgggata
    15661 aaacctaaaa caatgagtag tgcaaccatg agtgcaatat aggtagttgt tcttgtattc
    15721 atttaaatta attccttttt agtatgattt agttgtaatt tttccaagaa ttcaatatcg
    15781 gtatgttgat tgcgaccgtc ctctgttagg taatttccca aaaaagttga gtcaacaagt
    15841 aaggtgacta atgattcaaa attctcaaaa tggacctccc gtccagcact taatcttaat
    15901 tccttgaaag gaaaaacaaa acgcaacatg gccataattt ttaaacattt aatgggagtc
    15961 aaatagttat attttccaag aggtgttcct tcaacagcaa gtaaaaaatt gacagggata
    16021 gaataggggt tcagctcttt tagtctgaaa gctaatgtga tgagcccctc atcgctctct
    16081 cccataccac aaatgaatcc agaacaaaca tcaatgtctt cattgtgaat tctttctaaa
    16141 gtatcacaac gatctttgaa gctatgcgtc gttgcaatgt tggggtagtt ttcttcgggg
    16201 gtgttgagat tatgattgat acggtcaatt cctgcttgtt ttagctgatg aagttgttct
    16261 ctatccaaaa aaccagcggt aaggcatatt tctagaggga ggcttttttt gatttcaggg
    16321 atgacttgac ataactgctc aatttctctc ttgctaggct tagttccact catcgcaata
    16381 caaaagacgc tagcgccatt ttgatgagcg acaatagcct gttttagaat gacattttga
    16441 ggaagcagac caaatttatc aatgtcagca gtgctttctt tagattgaga acagtaacca
    16501 caattttctg cacaaagtcc ttgcttagca cttaataaaa cgttaaggcg aatattattt
    16561 cggctaactt gatttttaag gtataaggca gcagcgtaga tttcccacca gttctcatcg
    16621 ctatttagaa tttccagtgc ctgttcataa gaaatgctag tctttccaga taatatttcg
    16681 tcagccaaat ggatataatt tgtttgaaat gacatgatag cctcctttaa cgaaattaat
    16741 aatagcataa aaaaaa
//
LOCUS       DBJYSH010000041        16704 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.041, whole genome shotgun sequence.
ACCESSION   DBJYSH010000041 DBJYSH010000000
VERSION     DBJYSH010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 16704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16704
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.041"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            complement(<1..1263)
                     /locus_tag="ACUBV3_001023"
     CDS             complement(<1..1263)
                     /locus_tag="ACUBV3_001023"
                     /EC_number="2.4.99.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000687011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0015648 - lipid-linked peptidoglycan
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HHU3847530.1"
                     /translation="MKIDKRHLLNYSILIPYLILSILGLIVIYSTTSATLIQLGANPF
                     RSVINQGVFWAVSLVAIIFIYKLKLNFLKNSKVLTMAVLVEVFLLLIARFFTQEVNGA
                     HGWIVIGPISFQPAEYLKVIIVWYLAFTFARRQKKIEIYDYQALTKGRWLPRSLSDLK
                     DWRFYSLFMIGLVIAQPDLGNGSIIVLTVIIMYCISGIGYRWFSALLGLIVVGSTLFI
                     GTIAVVGVETMAKVPVFGYVAKRFNAFFDPFKDLTDSGHQLANSYYAMSNGGWFGRGL
                     GNSIEKLGYLPEATTDFVFSIVIEELGVIGAGFILALVFFLILRIMHVGIKAKDPFNS
                     MIALGIGAMLLMQVFVNIGGISGLIPSTGVTFPFLSQGGNSLLVLSVAIGFVLNIDAN
                     EKKELIMKEAEEQYKPQEKNEKIINLDAF"
     gene            complement(1369..4164)
                     /gene="ppc"
                     /locus_tag="ACUBV3_001024"
     CDS             complement(1369..4164)
                     /gene="ppc"
                     /locus_tag="ACUBV3_001024"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF000584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HHU3847531.1"
                     /translation="MSHPKLESSSNKEIITEEVGLLKQLLDEATQKLIGSESFDKIEK
                     IVSLSSTDDYTGLKETISALSNEEMVIVSRYFSILPLLINISEDVDLAYEINYKNNLN
                     QDYLGKLSTTIDVVAGHENAKDILEHVNVVPVLTAHPTQVQRKTVLELTSKIHDLLRK
                     YRDVKAGIVNQEKWYADLRRYIGIIMQTDTIREKKLKVKNEITNVMEYYNRSLIKAVT
                     KLTAEYKALAAKKGIHLENPKPLTMGMWIGGDRDGNPFVTAETLRLSAMVQSEVIINH
                     YIEQLNELYRNMSLSISLTEVSPELVTLANQSQDNSVYRENEPYRKAFNFIQDKLVQT
                     LLNLKVGSYPKEKFVSRQESSGIVGRYIKSHIAQVASDIQTEELPAYATAEEFKQDLL
                     LVKQSLVQHGQDSLVDGELACLIQAVDIFGFYLATIDMRQDSSINEACVAELLKSANI
                     VDDYSSLSEEEKCQLLLKELTEDPRTLSSTHTPKSELLQKELAIFQTARELKDQLGED
                     IIKQHIISHTESVSDMFELAIMLKEVGLIDANQARIQIVPLFETIEDLDNSRDIMTQY
                     LHYGLVKKWITTNNNYQEIMLGYSDSNKDGGYLSSGWTLYKAQNELTKIGEENGIKIT
                     FFHGRGGTVGRGGGPSYEAITSQPFGSIKDRIRLTEQGEIIENKYGNQDAAYYNLEML
                     ISASIDRMVTRMITNLNEIDNFRETMDGIVAESNAVYRNLVFDNPYFYDYFFEASPIK
                     EVSSLNIGSRPAARKTITEISGLRAIPWVFSWSQNRIMFPGWYGVGSAFKQFIEQDEA
                     NLAKLQTMYQKWPFFNSLLSNVDMVLSKSNMNIALQYAQLAGSKEVRDVFNIILNEWQ
                     LTKDMILAIEQHDNLLEENPMLHASLDYRLPYFNVLNYVQIELIKRLRSNQLDEDYEK
                     LIHITINGIATGLRNSG"
     gene            4373..6172
                     /gene="pepF"
                     /locus_tag="ACUBV3_001025"
     CDS             4373..6172
                     /gene="pepF"
                     /locus_tag="ACUBV3_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HHU3847532.1"
                     /translation="MELKKRSEFPENELWDLTALYKDRQDFLLAIEKALEDIKVFKKN
                     YEGKLNCVEDFTSALMEIEHIYIQMSHIDTYAFMPQTTDFSNEEFAQISQAGSDFATK
                     ANVLLSFFNTALANADIKILDSLENNPHFKATIRQAKIQKQHLLSPEVEKALTNLNEV
                     LNTPYDIYTKMRAGDFDMEDFEVDGKTYKNSFVTYENYFQNHENAEIREKSFRSFSKG
                     LRKHQNAAAAAYLAKVKSEKLIADMRGYDSVFDYLLSEQEVDRSMFDRQIDLIMDEFG
                     PVAQRFLKHIADVNGIEKMTFADWKLDIDNELNPEVSINDAYDLVMKSVAPLGKEYSQ
                     EVERYQKERWVDFAANANKDSGGYAADPYKVHPYVLMSWTGRMSDVYTLIHEIGHSGQ
                     FIFSDNHQSFFNTHMSTYYVEAPSTFNELLLSDYLENQFDTARQKRFALAHRLTDTYF
                     HNFITHLLEAAFQRKVYTLIEEGETFGAEQLNAIMKEVLTQFWGDAIEIDDDAALTWM
                     RQAHYYMGLYSYTYSAGLVISTAGYLNLKNNPNGAKEWLAFLKSGGSCTPLETALLIG
                     ADISTDKPLRDTINFLSNTVDQIINYSNLIKQG"
     gene            complement(6231..6620)
                     /locus_tag="ACUBV3_001026"
     CDS             complement(6231..6620)
                     /locus_tag="ACUBV3_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HHU3847533.1"
                     /translation="MKKYIKLFLLTVFATTLVACGQPSTSNKTTTSSTLEVGKVELVV
                     KEDTNVLSEKVVYHKGDTVLDVLKANYKVKEKDGFITSIDGISQDETKGLYWMFKVNN
                     KLAPKAANQIKVKKNDKIEFYQEVYKK"
     gene            complement(6604..7128)
                     /locus_tag="ACUBV3_001027"
     CDS             complement(6604..7128)
                     /locus_tag="ACUBV3_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HHU3847534.1"
                     /translation="MIKNSTLTKLTRVAILSALCVVLRYAFAPLPNIQPITAIFLITV
                     VLFDLKEGVATVTVTMLVSSFLMGFGPWVFLQIISFTLILCLWKFLIYPLTKAVCFGK
                     ITEVVLQTFFAGGLGVVYGVIIDTCFAWLYHMPWWTYVLAGLSFNMAHALSTCLFYPL
                     LLPILRRFRNEKIH"
     gene            7285..8133
                     /locus_tag="ACUBV3_001028"
     CDS             7285..8133
                     /locus_tag="ACUBV3_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001254333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HHU3847535.1"
                     /translation="MRRRIKPIVVAVFFSLFGLLLIIGHLHSTNTLKKELVEAKKTIP
                     SVKASKVPQKSTSSKDKEFVLKPIIDVSGWQLPKEIDYDTLSKNISGVVIRIFGGSKI
                     SKTNNAAYTTGIDKSFKTHIKEFQKRNIPVAVYSYALGSSVKEMKEEAQIFYKNAAPY
                     KPTFYWIDVEEETMSNMNKGVQAFRKELKRLGAKNVGIYIGTYFMTEQGISVKGFDAV
                     WIPTYGSDSGYYEAAPQTELKYDLHQYTSQGYLPGFNQPLDLNQIAVNKDKKKTYEKL
                     FGKVKD"
     gene            complement(8166..8783)
                     /locus_tag="ACUBV3_001029"
     CDS             complement(8166..8783)
                     /locus_tag="ACUBV3_001029"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000404311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HHU3847536.1"
                     /translation="MEILIAGGSGFLGKQIIKAALTKGHKVAYLSRHEGKGDIFKDPR
                     LTYIKGDITEADKIHLEHRNFDILIDCIGAIKPNQLDELNVKATQKAVALCHKNQIPK
                     LVYISANSGYSAYIKSKRKAEQIIKASGLDYLFVRPGLMYGEERPLSIFQAKCIKLFS
                     HLPFLGIVVQKVFPTKVVIVAEAIVTSLRKKPTQKILSIEELNNK"
     gene            9077..9550
                     /locus_tag="ACUBV3_001030"
     CDS             9077..9550
                     /locus_tag="ACUBV3_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HHU3847537.1"
                     /translation="MSKKNKIKKTLVDQILDKAKIEHDSLQLDALQGDLPNGIQKQDI
                     FKTLALIGDKTGPIIGILPLTEHLSEKKLAKISGNKKVQMIPQKDLQKITGYIHGANN
                     PIGIRQKHNYPIFIDTIALEKQELIVSAGEIGRSIRINSEVLADFVNAKFADIKE"
     gene            9572..10213
                     /locus_tag="ACUBV3_001031"
     CDS             9572..10213
                     /locus_tag="ACUBV3_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000675283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HHU3847538.1"
                     /translation="MKFYLVRHGKTQWNLEGRFQGANGDSPLLEEAIEELEELGQYLS
                     SIHFDAVYSSDLGRARDTVNILNDANSCPKEIHYTSQLREWALGTLEGCKIATMQAIY
                     PRQMTAFYQNPLQFKHDMFGAESLYQTTHRVESFLRSLASKNYDKVLIVGHGANLTAS
                     IRSLLGYQYGSLHYKDKLDNASLTIIETHDFKDFNCLTWNDKSYLRQEVKMTH"
     gene            complement(10249..11151)
                     /locus_tag="ACUBV3_001032"
     CDS             complement(10249..11151)
                     /locus_tag="ACUBV3_001032"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HHU3847539.1"
                     /translation="MITSIVFDVDDTIYDQQAPYRIAVEKCFPDFDMSAINQAYIRFR
                     HYSDIGFPRVMAGEWTTEYFRFWRCKETLLEFGYREIDEATGIYFQEIYEHELENITM
                     LDEMRMTLDFLKSKNVPMGIITNGPTEHQLKKVKKLGLYDYVDPKRVIVSQATGFQKP
                     EKEIFNLAAEQFDMNPSTTLYVGDSYDNDIMGAFNGGWHSMWFNHRGRSLKPGIKPVY
                     DVAIDNFEQLFGAVKVLFDLPDNKFIFDINDKSNPVLEMGLNNGLMMAAERLLESNMS
                     VDKVVILLRLTAKQEKVLRMKYAR"
     gene            11326..12816
                     /gene="lysS"
                     /locus_tag="ACUBV3_001033"
     CDS             11326..12816
                     /gene="lysS"
                     /locus_tag="ACUBV3_001033"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HHU3847540.1"
                     /translation="MSNQHIEELNDQQIVRREKMAALTEQGIDPFGKRFERTATSGQL
                     NEKYADKSKEDLHDIEETATIAGRLMTKRGKGKVGFAHIQDREGQIQIYVRKDSVGEE
                     NYEIFKKADLGDFLGVEGQVMRTDMGELSIKATHITHLSKALRPLPEKFHGLTDIETI
                     YRKRHLDLISNRDSFDRFVTRSKIISEIRRFMDSNGFLEVETPVLHNEAGGASARPFI
                     THHNAQDIDMVLRIATELHLKRLIVGGMERVYEIGRIFRNEGMDATHNPEFTSIEAYQ
                     AYADYQDIMDLTEGIIQHVTKTVKGDGPINYQGTEIKINEPFKRVHMVDAVKEITDID
                     FWKEMTLEEAQALAQEKNVPLEKHFTTVGHIINAFFEEFVEDTLIQPTFVFGHPVEVS
                     PLAKKNDTDPRFTDRFELFIMTKEYANAFTELNDPIDQLSRFEAQASAKELGDDEATG
                     VDYDYVEALEYGMPPTGGLGIGIDRLCMLLTDTTTIRDVLLFPTMK"
     gene            complement(12891..13361)
                     /gene="ribH"
                     /locus_tag="ACUBV3_001034"
     CDS             complement(12891..13361)
                     /gene="ribH"
                     /locus_tag="ACUBV3_001034"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HHU3847541.1"
                     /translation="MTIIEGQLVANEMKIGIVVSRFNELITSKLLSGAVDGLLRHGVS
                     EEDIDIVWVPGAFEIPYMARKMALYKDYDAIICLGVVIKGSTDHYDYVCNEVTKGIGH
                     LNSQSDIPHIFGVLTTDNIEQAIERAGTKAGNKGYDCALSAIEMVNLDKKLRER"
     gene            complement(13376..14569)
                     /locus_tag="ACUBV3_001035"
     CDS             complement(13376..14569)
                     /locus_tag="ACUBV3_001035"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HHU3847542.1"
                     /translation="MAFSPIKKLLQDIKSGEMVVLMDDENRENEGDLICAAEMVTKES
                     INFMAKFGKGLICLPLSNYYAEKLELAQMASHNTDNHETAFTISIDHLSTSTGISAED
                     RALTAKMVANDSSKAKDFRRPGHLFPLLAKEGGVLARNGHTEATVDLCRLAGLKECGL
                     CCEIMAEDGSMMRKDELLAFAQKHDLAIATIKQLQDYRRQEEGGVVREIEIQLPTQFG
                     HFKAYGYSEVVANKEHVALVKGDISSGEDVLCRLHSECLTGDVFHSLRCDCGEQLANA
                     LQQIEAEGRGVLLYMRQEGRGIGLINKLKAYHLQEEGLDTLEANLALGFEGDERDYGV
                     SAQLLKDLGINSINLLTNNPDKIQQLEAEGICVKNRVPLQVAATAYDLNYLKTKKEKM
                     GHLLD"
     gene            complement(14587..15237)
                     /locus_tag="ACUBV3_001036"
     CDS             complement(14587..15237)
                     /locus_tag="ACUBV3_001036"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HHU3847543.1"
                     /translation="MFTGIIEEMGQVSRIRNGIKSQQLSIDVPKLVPLLRKGDSVAVN
                     GVCLTVLDKSEKAFIADVMPESMMRTSLAALRLHSKVNLELALRSDSRLGGHFVLGHV
                     DGVGKIEKIQKDDIAVRFSIDAPPSIMSYIIEKGSVALDGISLTVVSFTEHSFEVSVV
                     PHTMAQTNLSLKKVGDLLNIEVDVLGKYAEKFLAPTNRTNHTSSVMDWSFLSENGY"
     gene            complement(15218..16327)
                     /gene="ribD"
                     /locus_tag="ACUBV3_001037"
     CDS             complement(15218..16327)
                     /gene="ribD"
                     /locus_tag="ACUBV3_001037"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HHU3847544.1"
                     /translation="MMKKEDYMALALKEAEKGMGFVAPNPLVGAVIVKDDRIISKGYH
                     KRFGDLHAERQAIKNADEDISGSTLYVTLEPCCHVGKQPPCTEALIKSGIKKVVVGSL
                     DPNPLVSGKGIALLRKEGLNVEVGILREECDALNERFIFHMTYKQPFVYLKYAMTLDG
                     KIATKTGDSKWISNEHSRQSVQKLRQKCSAIMVGINTVLADNPRLTCRIPKGEALVRI
                     VCDSQLKIPLDSYLVKSAKTIPTWIATCSDNLAQQQTLKEMGCRLIKVPRKDGKLDLK
                     VLMTILGQEGIDSLLIEGGSSLHFSALEAGIVNRLIVFIAPKIIGGLKAKTAISGEGL
                     DWLNQAFRVKDIELSRMDSDVVIEGKVEHYVYRNY"
     regulatory      complement(16521..16635)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
BASE COUNT         5460 a         3244 c         2599 g         5401 t
ORIGIN      
        1 aaaggcatca aggttgatta ttttttcatt tttttcttgt ggcttatatt gctcttctgc
       61 ttctttcatt atgagttcct ttttctcgtt agcatcaata ttaagcacga atccgatagc
      121 tacagaaagt accaagaggc tgttccctcc ttgggataag aaaggaaatg tcactccagt
      181 tgaggggatt aacccagaaa taccaccaat gttaacaaaa acttgcatca atagcattgc
      241 tccaatacca agagcgatca ttgagttgaa aggatcttta gcctttatac ctacgtgcat
      301 aatacgtaaa attaagaaga aaaccaaagc taaaataaac cctgcaccaa taactcctaa
      361 ttcctcaata acaattgaaa agacaaagtc tgttgttgct tctggtaagt accctaattt
      421 ttcaatagaa ttcccaagtc ctcgaccaaa ccagccaccg ttgctcattg cataatatga
      481 attagccaac tggtgtcctg aatctgttaa atctttaaat gggtcaaaaa aagcattaaa
      541 tcgcttagct acataaccaa agacagggac tttagccatt gtctcaactc caacaactgc
      601 aatagttccg ataaataaag ttgaccccac gacgattaga ccaagtaaag ctgaaaacca
      661 tcgataacca ataccactaa tacagtacat aataatgacc gttagaacaa ttatcgaacc
      721 atttcctaaa tcaggttgtg caattaccaa accaatcata aataaagaat aaaatcgcca
      781 atcttttagg tcgcttagtg atctcggaag ccatctccct tttgtcaaag cttgataatc
      841 atatatttct atcttttttt gccttcgagc aaaagtaaac gctaaatacc atacaatgat
      901 aacttttaaa tattcagcag gttgaaaact aataggacca ataacaatcc aaccatgtgc
      961 tccattaact tcttgtgtga aaaagcgcgc tattagtagc aaaaatactt ctaccaatac
     1021 tgccatagtc aacactttag aatttttcaa aaaatttagt ttcagtttat agataaagat
     1081 aattgcaacc aaactgactg cccaaaagac accctggttg attactgaac gaaaagggtt
     1141 agctccaagc tgaattagag ttgcactagt tgtcgaataa attacaatta agcctagaat
     1201 agacaatatc aaatagggaa tgaggatgga ataattcaga agatgccttt tatcaatttt
     1261 catattgtat tatttccttt aatatatcaa aaaattacca ttatgcatta tatcatcttt
     1321 cgaagtaaag taaaagggag aatagcaatg atcctccctc tatcaatatt aacctgaatt
     1381 tcgtaaacct gttgcaatac cattaatagt aatatgaatc aacttctcat agtcttcatc
     1441 tagttggtta ctacgaagac gtttaatcaa ttcaatttga acataattca aaacattgaa
     1501 atatggcaaa cgataatcta aacttgcatg taacattggg ttttcttcta ataaattatc
     1561 atgctgttca atagctaaaa tcatatcttt agttaactgc cattcattaa ggatgatatt
     1621 aaaaacatct cgaacttctt tacttccagc taactgagca tactgaagcg caatattcat
     1681 atttgatttt gataaaacca tatctacatt agataaaaga gaattaaaga atggccattt
     1741 ttgatacatg gtttgtaatt ttgctaagtt tgcctcatct tgttcaataa attgcttgaa
     1801 ggctgaccca acaccatacc aacccggaaa cataatacga ttttgtgacc atgaaaatac
     1861 ccatggaatt gcacgtaaac ctgaaatttc tgtaattgtt ttacgtgctg ctggccgaga
     1921 accaatattt aaacttgata cctctttaat tggacttgct tcaaagaaat agtcatagaa
     1981 ataaggatta tcaaaaacta aatttctata aacggcatta ctttcagcca caataccatc
     2041 cattgtctca cggaaattat caatttcatt aaggttagta atcatacgtg ttaccatgcg
     2101 gtctatggaa gctgaaataa gcatttcaag gttataatag gctgcatctt gattaccata
     2161 tttattctca atgatttcac cttgctctgt taggcgaatt cgatctttga ttgaaccaaa
     2221 aggttgtgat gtgatagctt cataagatgg accaccacca cgaccaacag ttccaccacg
     2281 accgtggaaa aatgtgattt taataccgtt ttcttcacct atttttgtta gctcattttg
     2341 agctttatat agagtccaac cagatgataa atagccacca tctttattac tatctgaata
     2401 acctagcatt atttcttgat aattgttgtt agttgtaatc cattttttta ctaatccata
     2461 atgtagatac tgggtcataa tatcacgtga attatccaaa tcttcgatgg tttcaaataa
     2521 cggtacaatc tgaatgcgag cttgattggc atcaattagt ccaacttctt ttaacatgat
     2581 agctaactca aacatatccg aaacgctctc tgtgtgagaa atgatatgct gttttataat
     2641 gtcttcaccc aattgatctt ttaactcacg tgctgtttgg aaaatagcca attctttttg
     2701 aagtaactcg gatttaggag tatgcgtaga agataatgtt ctaggatctt ctgttaattc
     2761 ttttaagaga agttgacatt tttcttcttc tgaaagagaa ctgtaatcat ctacaatatt
     2821 ggcagacttc aataattctg ctacacaagc ttcattaata ctagaatctt gacgcatatc
     2881 aatagtagct aaataaaatc caaagatatc tactgcttga ataagacaag ctaattcgcc
     2941 atcgacaaga gaatcttgtc catgttgaac taaagattgc ttcacaagta acaaatcttg
     3001 tttaaactcc tcagcagtag cgtatgctgg tagttcttct gtctgtatgt cagaagctac
     3061 ttgagcgata tgtgatttta tataccttcc tacaatacca gaagattctt ggcgactaac
     3121 aaatttctct tttgggtaag aaccaacttt taaatttagt agggtttgta ctagtttatc
     3181 ttggataaaa ttgaaagctt tacgataagg ttcattttcc ctatatactg aattatcttg
     3241 tgactggttt gccaacgtaa caagttcggg agagacttca gttagactta ttgaaagtga
     3301 catattacga taaagttcat ttagctgctc aatataatgg ttaataatca cttcactttg
     3361 taccatagct gataatctta gtgtctcagc agttacaaag ggattgccat ctctatcacc
     3421 accaatccac atccccatag tcagaggctt aggattctct aagtgaatac cttttttagc
     3481 tgccagtgcc ttatattctg ctgttaattt ggtaactgct ttaataagag aacggttgta
     3541 atattccatt acattggtta tctcattttt gactttcaat tttttctcgc gaatagtgtc
     3601 tgtttgcatg ataatgccga tgtaacgacg caagtcagca taccattttt cttgattcac
     3661 gattcctgct ttgacatctc tgtatttacg taataaatca tgaatcttag acgttaattc
     3721 gagcaccgtt ttacgctgca cctgagtagg atgagctgtt agcacaggca caacattgac
     3781 gtgttcaaga atatctttag cattttcatg gccagctaca acatcaatag tcgttgataa
     3841 ctttcctagg taatcttggt taagattatt tttataatta atttcatatg ctaaatcaac
     3901 gtcttctgaa atattaatta agagcggcaa aatagagaag taacgagaaa caataaccat
     3961 ctcctcgttg cttaaagccg aaatagtttc cttcaaacca gtataatcgt cagttgatga
     4021 taatgaaact attttttcaa tcttgtcaaa tgattcacta ccaatcaatt tttgcgttgc
     4081 ttcatccaaa agctgcttca gtaaaccgac ctcttcggta atgatttctt tattgctact
     4141 actttctaat ttcgggtggg acaaaccatc acttcctctc ttaaatctca ctactatgat
     4201 accattttaa agtaaaaagt aaaccattct aggattttta gatagaattt tcagattttt
     4261 acttatttat gacagtaatc agtttttctt gttcgacttt tttgatatac tgaggataat
     4321 tgagattaac tgattaattg ttaattaaca actattatta aaggagtatc gcatggaact
     4381 taaaaaacgc tctgaattcc ctgaaaatga gctttgggat ctgactgctc tctacaaaga
     4441 tcgtcaagat tttttactgg ctattgaaaa agctcttgag gatatcaagg ttttcaaaaa
     4501 aaattacgag ggaaaactta attgtgttga ggattttact agtgccctta tggaaatcga
     4561 gcatatttat atccagatga gtcacattga tacctatgct tttatgcctc aaactactga
     4621 ctttagcaac gaagagtttg cacaaatttc tcaagcagga tcagacttcg caactaaagc
     4681 taatgtacta ctttctttct ttaatactgc cctagcaaat gcagatataa agattttaga
     4741 ttctttagaa aataaccctc atttcaaagc aactattcgc caagctaaaa ttcaaaagca
     4801 acacttatta agtccagaag tcgaaaaagc tttgacaaac ttaaatgaag tcctaaatac
     4861 accatatgat atttatacta aaatgcgtgc tggcgatttt gatatggaag attttgaagt
     4921 tgacggaaaa acctacaaaa atagttttgt aacttatgaa aattactttc aaaatcacga
     4981 aaatgcagag atacgtgaaa aatccttccg ttcattctcg aaaggtctac gtaagcacca
     5041 aaatgctgca gctgcggctt atcttgcaaa agtcaaatct gaaaagttaa ttgctgatat
     5101 gcgtggttac gatagtgttt ttgattatct tctttccgaa caagaagttg accgttcaat
     5161 gtttgacaga caaattgatc ttatcatgga tgaatttggt ccagtagctc aacgattcct
     5221 caaacatatc gccgatgtta atggtattga aaagatgact tttgcagatt ggaaattaga
     5281 cattgataac gaacttaatc ccgaagtaag tatcaatgat gcttatgact tagtaatgaa
     5341 atcagttgct cctttaggaa aagaatattc ccaagaggtt gagcgctatc aaaaagagcg
     5401 ttgggttgat tttgcagcta acgctaataa agattctgga ggatatgctg cggatcctta
     5461 caaagttcat ccctatgtac tcatgagctg gacggggcgt atgagcgatg tctacacact
     5521 tattcatgag attggacatt ctggtcaatt tatcttctca gataatcacc aaagtttttt
     5581 caatactcac atgtccactt actatgttga agctccatct acttttaacg aacttttact
     5641 tagtgactac ttagaaaacc aatttgatac agcacgtcaa aaacgctttg ctcttgcaca
     5701 tcgcttaact gatacttact tccacaattt cattactcac ttacttgaag ctgctttcca
     5761 acgtaaagtt tacactctaa ttgaggaggg agaaactttt ggagcagaac aattaaatgc
     5821 tattatgaag gaagtattaa cacaattctg gggagatgct attgaaatag atgacgatgc
     5881 tgctttgaca tggatgagac aagctcatta ctacatgggg ctctatagct atacttactc
     5941 tgctggttta gtaatctcga cagccggcta tctcaatctc aaaaataatc caaacggagc
     6001 aaaagagtgg ttagcattct tgaaatctgg tgggagctgt acccctttag aaacagccct
     6061 attaatcggt gctgatattt ctactgataa gcctctacgc gatactatta atttcttatc
     6121 taacactgta gatcaaatta ttaattatag taatcttatt aaacaaggct aaactacagt
     6181 ctttcaaaaa cataaaaaca agttggagat atctccaact tgtttttatc ttattttttg
     6241 taaacttcct gataaaattc aattttgtcg ttctttttaa ctttaatttg gtttgctgcc
     6301 ttaggagcta atttattatt gaccttgaac atccaataaa gccctttggt ttcatcttgg
     6361 ctgatgccat caattgaagt aatgaaacca tctttttctt taactttata gttagcttta
     6421 agcacatcca aaacagtgtc acctttatgg taaacaacct tttcacttaa cacgttcgta
     6481 tcttctttaa caaccaattc aaccttacca acttctaacg ttgagctagt tgttgtctta
     6541 ttcgatgtag atggttgccc gcaagctact aaagtcgtag cgaatactgt tagcaagaat
     6601 aatttaatgt attttttcat tcctaaatct ccttaaaatc ggtaacaaaa gaggataaaa
     6661 taaacaagtt gataaagcgt gtgccatatt aaaactaagt cctgctaata cataagtcca
     6721 ccaaggcata tgatataacc aagcaaaaca ggtatcaata atgacgccat aaacaacccc
     6781 taaccctcct gcaaagaaag tttgaagtac tacctccgtt attttaccaa aacatacagc
     6841 ttttgtcagc ggatatatca gaaatttcca caagcataaa attaaagtga aggaaatgat
     6901 ttgcaaaaag acccatggcc caaagcccat taaaaaagaa gaaactagca ttgttacagt
     6961 tacagtagct acaccttctt ttaaatcgaa taagacaact gtaatcaaaa aaatggcagt
     7021 aattggctga atgttaggca gaggcgcaaa agcatatctc aacacaacac acaaagcaga
     7081 taatatagct actctagtca atttagttaa agttgaattc tttatcatgt ctttaattat
     7141 acctcaaaac tatgactacc gcttaaaagt cttttttatt acaatttcat ttctaatgtt
     7201 acataaacga cctctttttt taaaagaacg taaataagcc tgtaaacttt gattttttaa
     7261 accttttaag ttacaatatc aataatgaga agaagaatta aacctattgt tgttgcggtt
     7321 tttttcagtt tatttggact tttattaatt attggtcatt tacattcgac aaatactttg
     7381 aaaaaagaat tagttgaagc taaaaagaca attccatccg taaaagcttc aaaagtaccg
     7441 caaaaatcaa catcatcgaa agataaagag tttgttctta aaccgattat cgatgtctct
     7501 ggttggcaac ttcctaagga gattgattac gatacgcttt caaaaaatat ttcaggtgtt
     7561 gttattcgta tctttggtgg atcaaagata tctaagacta ataacgctgc ttatacaact
     7621 ggaatcgata aatcgtttaa gacccatatc aaagaatttc aaaagcgaaa tatcccagta
     7681 gctgtctaca gttatgcact tggttcaagt gttaaagaaa tgaaagaaga ggctcagata
     7741 ttttataaga atgcagctcc ttacaaacca actttttatt ggattgacgt agaagaggag
     7801 acaatgtcta acatgaataa aggtgtccaa gcattccgaa aagagttaaa aagacttggt
     7861 gctaaaaatg ttggtatcta catcggtact tactttatga ctgagcaagg tatctctgta
     7921 aaaggatttg acgctgtttg gattccaact tatggtagcg attctggata ctatgaagca
     7981 gctccacaaa ctgaacttaa atacgattta caccaataca cctctcaagg ttatctacca
     8041 ggattcaatc aaccgcttga tttaaatcaa attgcagtta ataaagacaa gaagaaaact
     8101 tatgagaaac tttttggaaa agtaaaagat taaaagctac tgttaacagt agcttttttg
     8161 ataaactatt tattatttaa ttcttcaata gaaaggattt tttgagttgg ttttttccta
     8221 agcgaagtaa cgattgcttc tgccactatc acaaccttag ttggaaagac tttttgtaca
     8281 acaataccta agaaaggcaa atgactaaat aattttatac acttggcttg gaaaatcgag
     8341 agaggtcgct cttcaccata catcaaacct ggtcttacaa aaagataatc cagaccgctt
     8401 gctttgatta tctgctctgc cttcctttta cttttaatgt aagctgaata gccgctattg
     8461 gctgaaatgt aaactaactt tggtatttga tttttgtgac agagtgctac tgctttttgg
     8521 gttgctttaa cgttaagctc atctagttga ttgggcttaa tcgctccaat acagtcaatt
     8581 aatatatcaa aatttctatg ttctaaatga atcttatcag cttctgtaat atctccctta
     8641 atgtaggtta atctaggatc cttaaatata tcacctttac cttcatgcct tgataagtaa
     8701 gccaccttat gcccttttgt aagcgctgct tttattatct gcttccctag aaaaccacta
     8761 ccacctgcaa tcagtatttc catagttccc tctccttcat tttgtaaatt tagtatagaa
     8821 atttttatat gccttgtcaa agtaatcgtt taatacctga ccagaatatg atttcaagtc
     8881 attacattag gacacttcat atttagctat ttataaaact tctaatttaa cattccaaat
     8941 atactgttgc gatatttatt atcattattc cctgaaatgg tattcaagct tatttctaag
     9001 actcagtaga aatttactat aatcatcttt aaatgttata ataaatatga aacaaactta
     9061 ttagttaagg gtcattatga gtaaaaagaa taaaatcaaa aaaactttag ttgatcaaat
     9121 tttagataaa gcaaaaatcg aacatgatag tctacaattg gatgctttac agggagattt
     9181 acccaacggt attcaaaaac aagatatctt taagacatta gcgctaatcg gtgataaaac
     9241 tggtcccata ataggtatct tacctctcac cgagcattta tccgaaaaaa agttagctaa
     9301 aatttcagga aacaaaaagg tacaaatgat acctcaaaaa gatctccaga aaataacagg
     9361 ttatattcac ggagcaaata atccaattgg aattcgccaa aaacataatt accctatttt
     9421 tatcgatact atcgctttag aaaaacaaga actcattgtc tctgcaggag aaattggacg
     9481 ttctattcgt attaatagtg aggttcttgc agatttcgta aatgctaaat ttgccgatat
     9541 aaaggaataa taattaggat tggagaaaaa tatgaaattt tatcttgtta gacacggaaa
     9601 aacacaatgg aatttagaag ggcgtttcca aggtgctaac ggagactcac ctctccttga
     9661 agaagctatt gaagaattag aagaacttgg acaataccta tcatctattc attttgatgc
     9721 tgtttattct agcgacttag gtcgtgctag agatactgtt aatatcctca atgatgctaa
     9781 ttcatgccca aaagaaatcc actatacttc tcagcttcgt gaatgggcat tgggaacttt
     9841 agagggttgt aaaattgcaa ctatgcaagc tatctatcct cgtcaaatga ccgcatttta
     9901 tcaaaatccc ttacaattta aacatgacat gttcggtgca gaatctcttt atcagacaac
     9961 gcatcgtgta gagagtttct tacgttcttt agcttcaaaa aattacgaca aggtccttat
    10021 tgtaggacat ggagcaaatc taactgcgtc tattcgttcc ttactgggtt atcaatatgg
    10081 tagtttacac tacaaagata aacttgataa tgccagcctc acaatcatag aaactcatga
    10141 ttttaaagac ttcaactgtc tgacttggaa cgataaatct tatttaaggc aagaggtcaa
    10201 aatgacacac taaaataatc cactagattc atctagtgga ttatttaatt atctagcata
    10261 cttcatgcgt aatacttttt cctgttttgc agttaggcgc agtaaaataa caactttgtc
    10321 aacgctcata ttactctcaa gcagacgctc tgctgccatc attaaaccat tattaagtcc
    10381 catttcaagg actggattac ttttatcgtt gatatcaaaa ataaatttat tatcaggtaa
    10441 gtcaaacaac actttaacag caccgaataa ttgctcaaag ttatcaatag caacatcata
    10501 aactggttta attcccggtt ttaaagaacg tcctctatgg ttgaaccaca tagaatgcca
    10561 accaccatta aatgcaccca taatatcatt atcatatgaa tcacccacat aaagtgtagt
    10621 tgaagggttc atatcaaatt gctctgctgc taaattaaaa atctcctttt caggcttttg
    10681 aaaaccagtt gcttgactaa ctataacacg ttttggatca acatagtcat aaagtcccaa
    10741 ttttttaact tttttcaatt gatgttccgt agggccattt gtaataattc ccattggtac
    10801 attttttgat ttcaaaaagt caagtgtcat acgcatctca tcaagcattg taatattttc
    10861 aagttcatgt tcgtatattt cttgaaaata aataccagta gcttcatcaa tttcacgata
    10921 accaaactct aaaagcgttt ctttgcatcg ccagaaacga aaatattctg ttgtccattc
    10981 accagccatt actcgtggaa agccaatatc tgaataatga cgaaagcgaa tataagcttg
    11041 gttaatagcg ctcatatcaa aatcaggaaa gcatttctcc acagcaatac ggtaaggtgc
    11101 ttgttgatcg taaattgtat catcaacatc aaaaacaatt gaagtaatca tgattatcct
    11161 tttctttctg aattttcaat tgttaattat acctttttct acccttattt tcaaatggtg
    11221 aaaagtctat taaaaatgag aataacacca taaactcata aaataaatta tgaattatca
    11281 gtgtttttgt ggtacaatag gatgaatact aacttggagg aaattatgtc taaccaacat
    11341 attgaagaat taaatgacca acagattgtt cgtcgtgaaa aaatggctgc tttgactgaa
    11401 caaggcattg atccttttgg taaacgattt gaacgtacag caacatctgg tcaattaaat
    11461 gaaaaatacg ctgacaaatc aaaagaagac ttgcacgaca ttgaagaaac tgctacaatc
    11521 gctggtcgtt taatgactaa gcgcggtaag gggaaagttg gctttgcgca tatccaagat
    11581 cgcgaaggtc aaattcaaat ttatgtccgt aaggattctg ttggcgaaga aaactacgaa
    11641 atctttaaaa aagctgattt aggtgacttc cttggtgttg aagggcaagt aatgcgtaca
    11701 gatatgggag aactttctat caaggctact catattactc acctttcaaa agcactccgt
    11761 ccacttcctg aaaaattcca cggcctaact gatattgaaa caatctatcg taaacgtcat
    11821 cttgatttaa tctcaaatcg tgacagcttt gaccgttttg taacacgctc aaaaatcatt
    11881 tcagaaattc gtcgctttat ggatagcaat ggtttcttag aggttgaaac acctgttctt
    11941 cataatgaag ctggtggtgc ttctgctcgc ccatttatca cacatcacaa tgcacaagac
    12001 attgacatgg ttcttcgtat cgcaactgaa ttgcatctaa aacgtcttat tgtaggtggt
    12061 atggagcgtg tttatgaaat cggacgtatt ttccgtaatg aagggatgga tgcaacccac
    12121 aatccagagt tcacatctat tgaagcctac caagcctatg ctgactacca ggatattatg
    12181 gacttgacag aaggtattat ccagcatgtt acaaaaactg taaaaggtga tggaccaatc
    12241 aattatcaag gcacagaaat taaaattaat gaaccattca aacgtgttca tatggttgat
    12301 gcggttaaag aaatcactga tattgacttc tggaaagaaa tgactcttga agaagcacaa
    12361 gctttggcac aagaaaaaaa tgttcctctt gaaaaacatt tcacaacagt tggtcacatc
    12421 attaacgcct tcttcgaaga attcgttgaa gacactttaa ttcaaccaac ttttgtcttt
    12481 ggtcatccag tcgaagtgtc tccacttgct aagaaaaatg atacagaccc tcgttttaca
    12541 gaccgtttcg aactctttat catgactaaa gaatatgcta atgcttttac agaattaaat
    12601 gacccaattg atcagctatc tcgctttgaa gcacaagcat ctgctaaaga acttggcgat
    12661 gatgaagcaa ctggagttga ttacgattat gttgaagcac ttgaatatgg tatgccacct
    12721 actggtggac taggtattgg tatcgaccgt ctctgtatgc tgttaactga cacaacaact
    12781 atccgagacg tcctactttt ccctacaatg aaataagaat atattaaaac ccacccttat
    12841 ttaaggtggg ttttaatatt acctctactg aactttgtta ggtttctgat ttatcgctca
    12901 cgcaattttt tatcaaggtt taccatttca atagccgaaa gagcacaatc ataaccttta
    12961 tttcctgctt ttgttcctgc cctctcaata gcttgttcga tattatccgt tgttagaaca
    13021 ccaaagatat gagggatatc tgactggcta tttaaatgac caataccttt tgtcacctca
    13081 ttacaaacat aatcataatg atcagtgctg cctttaatga ctaccccaag acagataata
    13141 gcatcataat ctttatataa tgccattttt ctagccatat aggggatttc aaaagcaccg
    13201 ggtacccata caatatctat atcttcttct gaaacaccat gtctcaacag tccatctact
    13261 gctcctgata ataatttaga tgtaatcaat tcattgaaac gtgagactac gattcctatt
    13321 ttcatttcgt ttgccactaa ttgtccttca ataattgtca ttttttcttc ctcctttaat
    13381 ctaaaagatg tcccattttt tcttttttag tttttaaata attaaggtca taagctgttg
    13441 ctgccacctg taatggcact ctgtttttga cgcaaattcc ttctgcttca agttgttgga
    13501 ttttatctgg attattggtt agtaagttta tagaatttat acctaaatct tttaaaagtt
    13561 gagcactaac tccatagtct ctctcatctc cttcaaaacc taaagccaaa ttagcttcta
    13621 aagtatctaa gccttcttct tgcaaatgat aagcttttaa cttattaatc aaaccaattc
    13681 ctcgaccttc ctgtctcata taaagaagga cgccacgacc ttcagcctct atttgttgga
    13741 gtgcattagc cagttgttcc ccacaatcac atctcaagga atgaaagaca tcacctgtca
    13801 aacattctga gtgtaaacga cataaaacat cttcaccact actaatatcc cctttaacaa
    13861 gggcaacatg ttccttattt gccacaactt cactatatcc ataagcctta aaatggccga
    13921 attgcgttgg tagctgaatc tcaatttctc taaccacccc tccctcttct tgacgtctat
    13981 agtcttgcaa ttgttttatg gtagcgatag ctaaatcgtg cttttgagca aatgctaata
    14041 actcatcctt tctcatcata ctaccatctt cagccattat ttcacaacaa agtccacatt
    14101 cctttaatcc agctaaacga catagatcaa cggttgcttc agtatgtcca tttctagcta
    14161 aaacacctcc ttccttagct aacaagggaa ataagtgccc aggacgtcta aagtctttag
    14221 ctttgctact gtcgttagca accatcttag ctgtcaaagc tctatcttcc gcagaaatcc
    14281 cagtactagt tgatagatga tcaatggata tggtaaaagc tgtttcatga ttatcagtat
    14341 tatggctagc catttgagct aattctaact tttcagcata gtaattagac agaggtaagc
    14401 aaattaatcc tttcccaaat ttagccataa aattaatcga ctcttttgtc accatttcag
    14461 ccgcacaaat aaggtcccct tcattctctc tattttcgtc atccatcaaa actaccattt
    14521 cccctgattt gatgtcttgt aaaagttttt ttattggact aaaggccatt cgctttctcc
    14581 tctctcttag tagccatttt ctgataaaaa agaccaatcc attactgatg aagtgtgatt
    14641 agttctatta gtcggtgcta aaaatttctc agcatatttg cctaaaacat ccacttcgat
    14701 atttaataaa tccccaacct tttttaagga taaattagtt tgagccatcg tgtggggaac
    14761 gaccgaaact tcaaaagaat gctctgtaaa tgataccacc gttaggctaa tcccatctag
    14821 agccaccgaa cctttctcta taatataaga cataatactg ggcggagcat caattgaaaa
    14881 tctgacagca atatcatcct tttggatttt ttctattttt ccaaccccat ccacatgtcc
    14941 aagaacaaaa tgccccccta accttgagtc acttctgagt gctaattcta agttaacttt
    15001 agaatgaagt cgtaaagcag ctaagcttgt tcgcatcata ctttctggca tgacgtctgc
    15061 tataaaagct ttttcacttt tgtcaaggac tgtcaaacaa acaccattaa cagctacgct
    15121 atcccctttt cttaataaag ggactagttt gggaacatca atacttaatt gttgggattt
    15181 tatgccattc cgaatacgac tgacttgacc catttcctca ataattcctg taaacataat
    15241 gttctacctt tccttcaata acaacgtcag aatccatacg actaagttca atatctttta
    15301 ctctgaaagc ttggttaagc caatccaagc cttctccaga aatagctgtt tttgctttca
    15361 gacctcctat tattttgggt gcaataaaaa caattagtct gttaacaata ccagcttcta
    15421 aagctgaaaa atgtaaacta cttcctcctt caataagaag gctatcaatc ccttcttgtc
    15481 ctaaaatagt cattaatact ttcaaatcca gctttccatc ttttcttggg accttaatca
    15541 aacggcaacc catttctttt aatgtttgtt gctgagcaag attatctgaa catgttgcaa
    15601 tccatgttgg tattgtttta gcgctcttaa ctaggtaaga gtctaaaggt atcttcaact
    15661 gactatcaca tacgatcctc acaagcgctt cacctttagg aatacggcaa gttaaacgtg
    15721 gattatcagc taatacagta ttgataccga ccataatagc actacatttt tgcctcaatt
    15781 tctgaacaga ttgtcttgag tgctcattgg atatccactt agaatcgccc gtctttgtag
    15841 ctatcttccc atctaaagtc atagcatatt tcaaataaac aaaaggctgc ttataggtca
    15901 tgtgaaaaat aaatctttca ttcagagcat cgcattcttc tcttaggata ccaacttcaa
    15961 catttaaacc ttcttttctt aacaaggcaa tcccttttcc tgaaactaaa ggatttggat
    16021 ctaaacttcc aactactact tttttgatac cagatttaat aagagcttca gtacaaggtg
    16081 gttgcttacc cacatgacag catggttcta aagtcacgta cagagtactt ccagagatat
    16141 cctcatcagc atttttaata gcctgtcttt ccgcatgtaa atctccaaat cgtttatgat
    16201 aacctttgga aattatacga tcatctttaa caataacagc acctactaat gggttgggag
    16261 caacgaaccc catacctttc tcagcctctt tcaaggctaa tgccatgtag tcttcttttt
    16321 tcatcatttc ctttttcctt ctaaaataaa aacccttagc ttctaaaata gaaaactaag
    16381 gggagggatg agaagtcgtt gattatacta attagcatta tattttaggt agcacaaaaa
    16441 aactaaagac tccctataga aaactatagg gagtctctca ttaatccagc acaaataaac
    16501 tgcaagtagc agacaatcac tgaattcttc tctcatccag actttactgt cggtactgga
    16561 atcacaccag ttcatgctcg aaagctcgcg gactataccg ccggttagga ttttcacctg
    16621 accccgaaga aattctattt gacagttatt ttactaaact catttttatt tgtcaataaa
    16681 aataatgtgt tttttattat aaaa
//
LOCUS       DBJYSH010000042        16055 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.042, whole genome shotgun sequence.
ACCESSION   DBJYSH010000042 DBJYSH010000000
VERSION     DBJYSH010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 16055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16055
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.042"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            complement(35..1573)
                     /locus_tag="ACUBV3_001038"
     CDS             complement(35..1573)
                     /locus_tag="ACUBV3_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HHU3847545.1"
                     /translation="MSKQKVMATLLLSTLVLSLSSPLVTLAETINPETSLTMATASTE
                     SSSEAEKQEKTQPTDSETASPSAEGSISTEKTEIGTTETSSSNESSSSSSHQSSSNED
                     AKTSDSASTASTPSTNTTNSSQADSKPGQSTKTELKPEPTLPLVEPKITPAPSQIESV
                     QTNQNASVPALSFDDNLLSTPISPVTATPFYVEHWSGQDAYSHYLLSHRYGIKAEQLD
                     GYLKSLGIQYDSNRINGAKLLQWEKDSGLDVRAIVAIAVLESSLGTQGVAKMPGANMF
                     GYGAFDHDSSHASAYNDEEAIMLLTKNTIIKNNNSSFEIQDLKAQKLSSGQLNTVTEG
                     GVYYTDNSGTGKRRAQIMEDLDRWIDQHGGTPEIPAALKALSTASLADLPSGFSLSTA
                     VNTASYIASTYPWGECTWYVFNRAKELGYTFDPFMGNGGDWQHKAGFETTHSPKVGYA
                     VSFSPGQAGADGTYGHVAIVEEVKKDGSVLISESNAMGRGIVSYRTFSSAQAAQLTYV
                     IGHK"
     gene            complement(1666..2766)
                     /gene="alr"
                     /locus_tag="ACUBV3_001039"
     CDS             complement(1666..2766)
                     /gene="alr"
                     /locus_tag="ACUBV3_001039"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HHU3847546.1"
                     /translation="MISSYHRPTRALIDLEAIANNVKSVQEHIPSDKKTFAVVKANAY
                     GHGAVEVSKYIESIVDGFCVSNLDEAIELRQAGIVKMILVLGVVMPEQVILAKNENIT
                     LTVASLEWLRLCQTSAVDLSGLEVHIKVDSGMGRIGVRQLDEGNKLISELGESGASVK
                     GIFTHFATADEADNCKFNQQLTFFKDFISGLDNCPDLVHASNSATSLWHSETIFNAVR
                     LGVVMYGLNPSGTDLDLPYPINPALSLESELVHVKQLHDGSQVGYGATYQVTGDEFVG
                     TVPIGYADGWTRDMQGFSVIVNGELCEIIGRVSMDQMTIRLPQKYTIGTKVTLIGQQG
                     SCNITTTDVAQKRQTINYEVLCLLSDRIPRYY"
     gene            complement(2763..3122)
                     /gene="acpS"
                     /locus_tag="ACUBV3_001040"
     CDS             complement(2763..3122)
                     /gene="acpS"
                     /locus_tag="ACUBV3_001040"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000635015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HHU3847547.1"
                     /translation="MIVGHGIDLQEIEAITKAYERNQRFAERVLTEQELLLFKGISNP
                     KRQMSFLTGRWAAKEAYSKALGTGIGKVNFHDIEILSDDKGAPLITKEPFNGKSFVSI
                     SHSGNYAQASVILEEEK"
     gene            complement(3147..4154)
                     /locus_tag="ACUBV3_001041"
     CDS             complement(3147..4154)
                     /locus_tag="ACUBV3_001041"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HHU3847548.1"
                     /translation="MGFHQLSDKINIEILKQKTSLDLEVSQKKLAKEEELKNIIKGED
                     QRFLVIVGPCSADNPKAVLTYAKRLAKLEAAFKDKMFLVMRVYTAKPRTNGDGYKGLV
                     HHSDKLGVFFQARKMHYDIIRETGLLTADELLYPEMLSVMDDLVSYYAIGARSVEDQG
                     HRFISSGIDAPVGMKNPTSGNLRVMLNAVYAAQNQQELFYQNKQVRTDGNLLSHVILR
                     GYHNADYRSIPNYHYENLLETITHYEETDLQNPFIVVDTNHDNSGKQFLEQIRIVKSV
                     LADRQWHTKIRNYVRGFLIESYLEDGRQDKPDVFGKSITDPCLGWDKTEMLIRDIYNS
                     L"
     gene            complement(4280..6808)
                     /gene="secA"
                     /locus_tag="ACUBV3_001042"
     CDS             complement(4280..6808)
                     /gene="secA"
                     /locus_tag="ACUBV3_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HHU3847549.1"
                     /translation="MANILRTVIENDKGELKKLDKIAKKVDSYADHMAALSDEALQAK
                     TPEFKERYQNGETLDQLLPEAFAVVREASKRVLGLYPYHVQIMGGIVLHHGDIPEMRT
                     GEGKTLTATMPVYLNAISGLGVHVITVNEYLSTRDATEMGEVYSWLGLSVGINLAAKS
                     PFEKREAYNCDITYSTNAEVGFDYLRDNMVVRQEDMVQRPLNYALVDEVDSVLIDEAR
                     TPLIVSGPVSSEMNQLYTRADMFVKTLNSDDYIIDVPTKTIGLSDTGIDKAENYFHLN
                     NLYDLENVALTHYIDNALRANYIMLLNIDYVVSEEQEILIVDQFTGRTMEGRRFSDGL
                     HQAIEAKESVPIQEESKTSASITYQNMFRMYHKLAGMTGTGKTEEEEFREIYNMRVIP
                     IPTNRPVQRIDHSDLLYPTLDSKFRAVVADVKERYEQGQPVLVGTVAVETSDLISRKL
                     VAAGVPHEVLNAKNHFKEAQIIMNAGQRGAVTIATNMAGRGTDIKLGEGVRELGGLCV
                     IGTERHESRRIDNQLRGRSGRQGDPGESQFYLSLEDDLMRRFGTDRIKVVLERMNLAE
                     DDTVIKSKMLTRQVESAQRRVEGNNYDTRKQVLQYDDVMREQREIIYANRREVITAER
                     DLGPELKGMIKRTIKRAVDAHSRSDKNTAAEAIVNFARSALLDEEAITVSELRGLKEA
                     EIKELLYERALAVYEQQIAKLKDPEAIIEFQKVLILMVVDNQWTEHIDALDQLRNSVG
                     LRGYAQNNPIVEYQSEGFRMFQDMIGSIEFDVTRTLMKAQIHEQERERASQHATTTAE
                     QNISAQHVPMNNESPEYQGIKRNDKCPCGSGMKFKNCHGLRCLQ"
     gene            complement(6917..7864)
                     /gene="manA"
                     /locus_tag="ACUBV3_001043"
     CDS             complement(6917..7864)
                     /gene="manA"
                     /locus_tag="ACUBV3_001043"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HHU3847550.1"
                     /translation="MSEPLFLEASMHDKIWGGTKLRDEFGYDIPSETTGEYWAISAHP
                     NGVSRVKNGRFKGCFLDKLYQGEKSLFGNPDDTVFPLLTKILDANDWLSVQVHPDDAY
                     ALKHEGELGKTECWYIISADEGSEIIYGHNAKTKEELRQMIESGDWEHLLTRIPVKSG
                     DFYYVPSGTMHAIGKGILILETQQSSDTTYRVYDFDRPDASGKLRDLHIEQSIDVLTI
                     GKPANTVPANMKLKHLSSTLLVSNDFFTVYKWEISGVTNFKQFAPYLLVSVLDGAGHI
                     TVDNKVYTLKKGDHFILPNDVVKWDIDGQLEIIASHVKK"
     gene            complement(7982..8863)
                     /gene="scrK"
                     /locus_tag="ACUBV3_001044"
     CDS             complement(7982..8863)
                     /gene="scrK"
                     /locus_tag="ACUBV3_001044"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HHU3847551.1"
                     /translation="MTKLYGSIEAGGTKFVCAVGDEELKVVEKIQFPTTTPQETIKKT
                     VDFFKRFEKKLEAVAIGSFGPIDIDKKSKTYGYITTTPKLHWANVNLLGLISKDFNVP
                     FYFTTDVNSSAYGEVIARNNIDSLVYYTIGTGIGAGAIQKGEFIGGTGHTEAGHTYMA
                     MHPQDQANDFKGICPFHNSCLEGLASGPTLEARTGIRGELIEENSMVWDVQAYYIAQA
                     AIQATVLYRPQVIVFGGGVMAQKHMLRRVRQTFATLLNGYLPVPNLSDYIVTPAVEEN
                     GSATLGNFALAKKISKG"
     gene            complement(8931..10850)
                     /locus_tag="ACUBV3_001045"
     CDS             complement(8931..10850)
                     /locus_tag="ACUBV3_001045"
                     /EC_number="2.7.1.211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-specific PTS transporter subunit IIBC"
                     /protein_id="HHU3847552.1"
                     /translation="MASNTEIAKQVINAIGGASNVRSVAHCATRLRVMVKDETVIDKN
                     TVENIEKVQGAFFNSGQYQIIFGTGTVNKIYDEVVAQGLPTSSTSDQKAEAAKQGNAF
                     QRAIRTFGDVFVPLLPAIVATGLFMGIRGAINNDTVLALFGTTSKAFSSSNFYTYTVV
                     LTDTAFAFFPALISWSAFRVFGGNPVIGLVLGLMMVNSALPNAWAVASGDAHPIKFFG
                     FIPVVGYQNSVLPAFFVGLLGAKLEKWLHKKIPDVLDLLLVPFLTFTVMSILALFVIG
                     PIFHSVENYVLAGTKFVLNLPLGLSGLILGGVHQIIVVTGVHHIFNLLEAQLIAADGK
                     DPFNAIITAAMTAQAGATLAVGVKTKNKKLKALAFPAALSAGLGITEPAIFGVNLRFG
                     KPFIMGLIAGAAGGWLASILKLAGTGFGITIIPGTLLYLNGQIVKYLIMVIGTTALAF
                     VLTYMFGYEDKDEKVVAEVSPLVEETDDNPTITQTSQLRAETIVSPLDGQVIALDTVS
                     DPVFSSGIMGDGLAIKPSGNTIYSPVDGFVQIVFETGHAYGIKSDKGAEILIHIGIDT
                     VTMNGTGFTSKVKADQKVKKGDILGTFDSAKIAEAGLDNTAMIIVTNTADFADVIPLV
                     KEGVVTTGDMIIDVK"
     gene            11035..12474
                     /locus_tag="ACUBV3_001046"
     CDS             11035..12474
                     /locus_tag="ACUBV3_001046"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004564 - beta-fructofuranosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HHU3847553.1"
                     /translation="MNLPTEIRYRPYDEWTEEDKENIVKNVSKSPWRATYHLEAKTGL
                     LNDPNGFSYFNGKFHLFYQNWPFGAAHGLKQWVHTESDDLVHFKETGIKLKPDHVNDS
                     HGAYSGSALAIDDKLFLFYTGNVRDMKWNRDPRQIGAWMTSDGKITKFDKVLISQPND
                     VTEHFRDPQIFNYDNQFYAVIGAQNSKKCGFIKLYKALNNDIHHWEFVGDLDFGGTGS
                     EYMIECPNIIFVKGKPVLLYSPQGLDKNELDYQNIYPNTYKIGQYFDANSSKIVEPSP
                     IYNLDYGFEAYATQGFNTSDGRAFIVSWIGLPDIDYPSDQFDYQGAMSLVKELSIKNG
                     NLYQYPVPAMKNLRQHQAEFKTQLQTNNTYELELLVPRNDLSSFVLFANPKGQGLSIT
                     IDTAKGKVIVDRSQAGQQYATEFGTSRQCNIPKDATSVNVFIDKSIFEIFINKGEKVF
                     TGRVFPDAEQSGIQLKEGHVHGKYFELKY"
     gene            12476..13438
                     /locus_tag="ACUBV3_001047"
     CDS             12476..13438
                     /locus_tag="ACUBV3_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HHU3847554.1"
                     /translation="MVAKLTDVAALAGVSPTTVSRVINKKGYLSQKTVTKVNEAMRTL
                     GYKPNNLARSLQGKSAKLIGLIFPNIRNIFYAELIEHLEIELFKHGYKTILCNSEKDP
                     IKEKEYLEMLGANQVDGIISSSHNLGIDDYEKVEAPIVAFDRNLAPHIPIVSSDNFFG
                     GKMAAQTLKKHGCQKMIMITGNDNSDSPTGLRRLGFSYESKESKVITVTNGLSNMRRE
                     MELKSIISTHKPDGIFTSDDLTALLVIKLISQLGLSIPEDIKVIGYDGTSFIQDYVPH
                     LTTIKQPIREIAQLMVEILLAKIEGQKTNKDYILPVSLIPGSSV"
     gene            complement(13525..13959)
                     /gene="nusB"
                     /locus_tag="ACUBV3_001048"
     CDS             complement(13525..13959)
                     /gene="nusB"
                     /locus_tag="ACUBV3_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000204941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HHU3847555.1"
                     /translation="MTSVFKDSRRDLRERAFQTLFSLETGGEFIDAAHFAYGYDKTVS
                     EDKVLEVPIFLLNLVNGVVDHKDELDTLISSHLKSGWSLERLTLVDKSLLRLGLYEIK
                     YFDETPDRVALNEIIEIAKKYSDETSAKFVNGLLSQFITNEN"
     gene            complement(13952..14341)
                     /locus_tag="ACUBV3_001049"
     CDS             complement(13952..14341)
                     /locus_tag="ACUBV3_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HHU3847556.1"
                     /translation="MTTENLGEIVISPRVLEVITGIAATKVDGVHSLRNKAVTDSLSK
                     KSLGRGVYLKNEEDDTVAADIYVYLQYGVNVPAVSIAIQQAVKTAVYDMAEVKISSVN
                     IHVEGIVPEKTPKPDLKSLFDEDFLDD"
     gene            complement(14430..14990)
                     /gene="efp"
                     /locus_tag="ACUBV3_001050"
     CDS             complement(14430..14990)
                     /gene="efp"
                     /locus_tag="ACUBV3_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HHU3847557.1"
                     /translation="MIEASKLKAGMTFETADGKLIRVLEASHHKPGKGNTIMRMKLRD
                     VRTGSTFDTSYRPEEKFEQAIIETVPAQYLYKMDDTAYFMNNETYDQYEIPTVNIENE
                     LLYILENSEVKIQFYGTEVIGVQIPTTVELTVAETQPSIKGATVTGSGKPATMETGLV
                     VNVPDFIEAGQKLVINTAEGTYVSRA"
BASE COUNT         5056 a         3157 c         2587 g         5255 t
ORIGIN      
        1 aaaggcgcct caagagcgcc tttattatgt ctaattattt atggccaata acataagtta
       61 attgtgcagc ttgtgctgaa ctaaaagtac ggtaagagac aataccacgt cccattgcat
      121 tagattctga aataagaact gaaccatctt ttttaacttc ttcaacaata gctacgtgac
      181 cgtaagtgcc atcagcacca gcttgtcctg gtgaaaatga tacagcatag cctacttttg
      241 gtgaatgtgt tgtttcaaaa ccagccttat gttgccaatc tccaccatta cccataaatg
      301 gatcaaatgt ataacctaac tctttagcgc ggttaaagac ataccatgta cattcacccc
      361 atggataagt tgatgcaata tagctagctg tgttaactgc tgttgataag ctaaaaccac
      421 ttggtaaatc tgctaaactt gctgtcgata aagctttcaa ggcagcagga atttctggtg
      481 tccctccatg ttgatcaatc cagcggtcta aatcttccat aatctgggca cgacgtttac
      541 cagttccaga gttatctgta taataaacac caccctcagt aactgtatta agttgtccag
      601 aagataattt ctgtgctttc aaatcttgga tttcaaagct agagttgtta tttttaataa
      661 ttgtattttt tgtcaacaac ataattgctt cttcatcatt ataagcacta gcatggctag
      721 agtcatgatc aaaggcacca taaccaaaca tattagcacc tggcatctta gccactcctt
      781 gagttcccaa tgaactttca aggacagcaa tagctacaat agcacggaca tctaaaccac
      841 tatctttttc ccattgtaat aacttagcac cattgatacg attagaatca tattgaatcc
      901 ctaaagattt taagtaccca tctaattgtt cagctttgat accgtaacga tgtgacaata
      961 aatagtgaga gtaggcatcc tgaccagacc agtgttctac gtagaatggc gttgctgtca
     1021 ctggtgaaat cggtgttgat aataaattat catcaaagga taaagcagga acagaagcat
     1081 tctgatttgt ctgaacactt tctatctgag acggagcggg agttatttta ggctctacta
     1141 atggtaaggt aggctcaggt tttaattcag tctttgttga ttgacctggc ttactgtctg
     1201 cttgactact gtttgtagta ttagtgctag gagtagatgc tgttgaagca gaatcagatg
     1261 ttttagcatc ttcgttggaa gaagattgat gtgatgaact tgatgatgat tcattgcttg
     1321 atgatgtctc tgtcgtacca atctctgttt tttctgttga gatacttcct tcggctgaag
     1381 gtgaagcagt ttctgaatct gtaggttgtg ttttttcctg tttctctgct tcagaagaac
     1441 tttctgttga tgctgttgcc attgtcaggc ttgtttctgg attaatagtt tctgctaagg
     1501 tcactaaagg tgatgatagc gataagacta aagtggataa caacaaagtt gccattactt
     1561 tttgttttga catatactaa tgatttcctt ctataaaaat tgtcactgat tagtttaaca
     1621 tatttttaaa ttttttaaaa cagttaacta ctgtgtcatt tgtttttaat aatatctcgg
     1681 aatacgatcg cttaaaagac aaagtacttc ataattaatc gtctgtcttt tttgagcgac
     1741 gtccgtagtt gtaatgttac aactaccttg ttgaccaatc aaggttactt tagttccgat
     1801 agtatatttt tgaggtaaac ggatagtcat ttgatccatc gagacacgcc caataatttc
     1861 acaaagttct ccatttacta taactgaaaa tccttgcata tctcgtgtcc atccatcagc
     1921 gtagccaatc ggtactgttc caacaaattc gtctccagta acttgatagg ttgcgccata
     1981 gccaacttga gaaccatcgt gtagttgttt aacatgtact agctcagact ctaaacttaa
     2041 agcagggttt atagggtaag gtaagtctaa atcagttcca cttggattta agccgtacat
     2101 aacaacaccc aatctaacgg cattaaaaat tgtctcacta tgccaaaggc ttgtagcaga
     2161 attactagca tgaacaaggt ccggacaatt atctaatcct gaaataaagt ctttaaaaaa
     2221 agtaagttgt tgattgaatt tacagttatc ggcttcatca gctgttgcaa aatgggtgaa
     2281 aatccccttg acagaggcac cactttcccc caattcagaa attagcttat ttccttcatc
     2341 taactggcga acacctatac gtcccattcc actatcaact ttgatatgta cttctagacc
     2401 agataaatct actgctgatg tctgacataa acgtaaccac tccaagctag caactgttag
     2461 agtaatattt tcatttttgg ctagtataac ctgctcaggc ataacaacac ctaataccaa
     2521 tatcattttt acaattcctg cttgacgtag ctcaatagcc tcatctaaat tggatacgca
     2581 aaaaccatct acaatagact caatgtattt tgaaacttca acagcaccat gcccataagc
     2641 attagcctta actacagcaa atgttttttt atcacttgga atgtgctctt gtacagattt
     2701 aacgttattt gcaatcgcct ctaaatctat caaggctctg gttggtctat gataacttga
     2761 aatcattttt cttcctccaa aataacacta gcttgtgcat aattaccact atgagatatt
     2821 gaaacaaaag attttccatt aaacggttct tttgtaatca aaggcgctcc tttatcatcc
     2881 gataaaattt cgatatcatg aaaattaact ttcccaattc ctgttccaag tgctttgcta
     2941 taagcctctt ttgctgccca tcgccctgtt aaaaaagaca tctgacgctt gggattggaa
     3001 attcctttaa aaagaagcaa ttcttgttcg gtcaaaacgc gttctgcaaa acgttgatta
     3061 cgctcatatg ctttagtaat cgcctctatc tcttgtaaat caattccgtg tccaacaatc
     3121 atgtgtaact cctataagtt cgatacttac aaactattgt aaatatccct aattagcatt
     3181 tctgttttat cccaacctag acatggatct gtgatagact taccaaaaac atctggttta
     3241 tcttgtcttc catcttctaa ataagattct atcaaaaaac cacgaacata gtttctaatt
     3301 ttagtatgcc actgtcggtc tgcaagtact gatttcacaa tgcgaatttg ttctaaaaat
     3361 tgcttacctg aattgtcatg atttgtatca acaacaataa agggattttg caaatcagtc
     3421 tcttcataat gagtaatagt ttctaaaagg ttttcataat gataattagg gatacttcga
     3481 tagtcggcat tatgatatcc tctcaaaata acatgtgaca gcaaattacc atctgttcta
     3541 acttgcttat tttgatagaa taattcctgt tgattttgtg ctgcatagac cgcattaagc
     3601 atgactctta aattaccaga cgttggattt ttcataccta ctggtgcatc tatacctgaa
     3661 gatataaaac ggtgtccctg atcttccact gaacgcgcac caatagcata gtaagaaact
     3721 aaatcatcca tcactgataa catctctgga taaagtaatt catcagctgt caggagtcca
     3781 gtctctcgaa tgatgtcata gtgcatctta cgtgcttgaa aaaaaactcc tagtttgtca
     3841 gaatggtgaa ctaagccttt ataaccatca ccattcgttc ttggcttagc ggtataaact
     3901 ctcataacca aaaacatttt atctttaaag gcagcttcca atttagctaa acgctttgca
     3961 taagttagca ccgcctttgg attatccgca gaacaaggac ctactattac tagaaaccgc
     4021 tgatcctcac cttttatgat attttttaac tcttcttctt tggctaattt tttttggcta
     4081 acttctagat ctaagctagt tttctgcttc aatatttcaa tattaatttt atcacttaac
     4141 tgatgaaatc ccatgttttt tcctaataca acctaagtct attaataagc tctgctatac
     4201 aatatagtcg tcatactatt agacaagtaa tgataaaaca gtcttctcag actgttttat
     4261 ttttatttta aaatcattgt tattgtaaac atctcagacc atgacaattt ttaaacttca
     4321 tgccagagcc acaaggacat ttatcattac gtttaatccc ttggtattct ggtgattcat
     4381 tattcatagg tacatgttgt gccgagatat tttgctctgc tgttgtcgta gcatgttgag
     4441 aagcacgttc acgctcttgt tcatgaattt gagctttcat caatgtacgt gtcacatcaa
     4501 actcaatcga accaatcata tcttggaaca tacggaaacc ttctgattga tactcaacga
     4561 ttgggttatt ttgagcataa ccacgaagtc caacggagtt acgtaattga tctaaggcat
     4621 caatatgctc tgtccattga ttatccacta ccatcaagat taagactttt tggaattcga
     4681 taatagcttc tggatctttt aatttagcaa tttgttgctc ataaacagct aaggcacgtt
     4741 cataaagaag ttctttaatt tcagcctctt ttagaccacg aagttctgaa actgtgatag
     4801 cttcttcatc taataacgcc gatcttgcaa aattaacaat agcttctgct gcagtatttt
     4861 tatcgcttct gctatgtgca tcaactgctc ttttaatcgt acgtttaatc atccctttga
     4921 gctctggtcc taaatcacgc tcagcagtaa tcacttctct acggttagca taaataatct
     4981 cacgttgttc acgcataaca tcatcgtatt gcaagacttg tttacgtgtg tcatagttat
     5041 taccttcaac gcggcgttga gctgattcaa cttgtcgtgt caacattttt gatttaataa
     5101 ctgtatcatc ttcagctaaa ttcattcgct caagaaccac tttaatacga tctgtaccaa
     5161 agcgacgcat caaatcatct tctaacgaaa gatagaattg tgattcacct ggatctcctt
     5221 gacgacccga acgaccacga agctggttat caatacgacg gctttcatga cgctctgtac
     5281 caataacaca taaaccacca agttcacgaa ccccttcacc aagcttgata tcagtaccac
     5341 gaccagccat gttggttgcg attgtaacag caccacgttg cccagcattc ataatgattt
     5401 gagcttcttt aaagtgattt ttagcattca aaacttcatg tggtactcca gcagcaacta
     5461 attttctaga aattaagtct gacgtttcaa cagctactgt accaaccagt acaggttgtc
     5521 cttgttcata acgttctttg acatctgcta ccaccgctct aaatttagag tcaagagttg
     5581 gataaagtag atctgaatga tctatacgtt gaacaggacg atttgttggg ataggaataa
     5641 cgcgcatatt ataaatttca cgaaactctt cttcctcagt cttaccagta ccagtcattc
     5701 ctgctaattt atggtacata cggaacatat tttggtaagt aattgaagcg ctcgtttttg
     5761 attcttcttg gattggcact gactcttttg cttcaatagc ttggtgtaag ccatctgaga
     5821 aacgacgtcc ttccatcgta cgaccggtaa attggtccac aattaaaatc tcttgttctt
     5881 cactaaccac ataatcaata tttaacaaca taatatagtt agctcgaaga gcgttatcaa
     5941 tgtagtgagt taaggcaaca ttttcgagat catacaagtt atttagatgg aaatagtttt
     6001 cagccttgtc aatacctgta tctgataaac caattgtttt tgttggaaca tcaatgatat
     6061 aatcatcgct attaagcgtt tttacaaaca tgtccgcacg tgtatacagt tggttcatct
     6121 cagagcttac aggacctgaa acaatcaatg gcgtcctagc ttcatcgatt aatacagagt
     6181 caacctcatc aaccaaagca tagttgagtg ggcgttgcac catatcttct tggcgaacaa
     6241 ccatattgtc tcgaaggtag tcaaatccga cttctgcgtt agttgagtat gtgatatcac
     6301 aattgtaagc ttcgcgtttt tcaaatggtg acttagctgc taagttaatc cctacagata
     6361 atccaagcca actataaacc tcacccattt cggtcgcatc acgtgttgat aagtactcat
     6421 taacagtaat aacatgaaca cctaatcctg agatagcatt tagataaaca ggcattgtcg
     6481 ctgttaaagt ttttccctca ccagtacgca tttctggaat atcaccatgg tgaagaacga
     6541 taccacccat aatttggacg tggtatgggt ataaacctaa tacacgttta gaagcttcac
     6601 gaactaccgc aaacgcttcc ggtaacaatt gatctaaagt ttcaccattt tgataacgtt
     6661 ctttaaattc tggagttttt gcttgtaaag cttcatctga taacgcagcc atatgatctg
     6721 cgtaagagtc aactttctta gctattttat ctaatttttt taattcaccc ttgtcatttt
     6781 caataacagt gcgtagaata tttgccattc taattccttt cgatactctt atcaccacat
     6841 tttaccataa ataatgttgt ctttcaataa atggtcgttt taatcgagat taaaaagagt
     6901 ggtatcccca ctctcattat tttttaacat gacttgctat aatttctaac tgtccatcaa
     6961 tatcccattt gacaacatca tttggcaaga taaagtggtc acctttttta agtgtataaa
     7021 ctttgttatc tactgtaatg tgacccgctc cgtctaagac actaaccaaa aggtagggtg
     7081 caaattgttt aaagttggta acaccagaaa tttcccattt ataaactgta aagaaatcat
     7141 ttgaaactaa taaagttgaa gatagatgtt ttaacttcat attggcagga acagtattgg
     7201 caggtttacc aatcgtgaga acatctattg attgttcaat gtgtaaatca cgtaattttc
     7261 ctgaagcatc tggacgatca aaatcataaa cacgataagt agtatcacta gattgctgtg
     7321 tttctaaaat taatatccct ttacctatag catgcattgt cccacttgga acgtagtaaa
     7381 aatcccctga tttaacagga attcgggtta aaagatgttc ccaatctcca gattcaatca
     7441 tttggcgaag ttcttcttta gttttagcat tatgaccata gataatttca gatccttcat
     7501 cagcagaaat aatataccaa cattctgttt taccaagttc accttcatgt ttaagagcat
     7561 aagcatcatc cggatgaacc tgtacactaa gccagtcatt agcatctaag attttagtta
     7621 gtaaaggaaa gacagtatca tcagggttgc caaataagct tttttcacct tgatacagct
     7681 tatctaaaaa acaaccttta aagcgaccat tcttaactct tgagacaccg ttaggatgag
     7741 ctgaaatagc ccaatattct cctgttgttt cacttggaat atcatagcca aattcatccc
     7801 ttaactttgt tccaccccag attttatcgt gcatgcttgc ttctaaaaat aatggttccg
     7861 acataagaat cccctatctt ttgtaaaatt caagcctatt atagcatttt atccatcata
     7921 tcgcttactt tttttaatct tttatttgct aactaaacta agtagtggct taagggtttt
     7981 attaaccttt tgatattttc tttgctaaag caaaatttcc taacgttgca gaaccatttt
     8041 cttctacagc tggtgtcaca atataatcag acaaattggg aactggtaaa tacccattta
     8101 ataaagttgc aaatgtctgg cgaacacgtc ttaacatgtg tttttgtgcc atgacgcctc
     8161 caccaaatac aatgacttgt ggacgatata atacagttgc ttgtattgca gcctgagcaa
     8221 tataataggc ttgaacatcc caaaccattg aattttcttc aatcaattca ccacgaatac
     8281 cagtcctagc ttctagagtt ggaccagatg ccaaaccttc taaacagcta ttatgaaatg
     8341 gacagatacc tttaaaatca tttgcttggt cctgcggatg catcgccata taagtatgac
     8401 ctgcttcagt atgaccagtt cctcctataa attcaccctt ttgtatagct cctgcaccaa
     8461 ttcctgtacc aatggtgtaa taaacaagtg aatcaatgtt gttacgagct ataacttcac
     8521 cataggcaga gctattaaca tctgttgtga aataaaatgg aacattgaaa tctttagaaa
     8581 ttaagccgag taaattaaca ttagcccaat gtaactttgg ggtcgttgtg atataaccgt
     8641 atgtctttga ttttttatca atatcaatgg gaccaaaaga accaatagca actgcctcta
     8701 acttcttttc aaatctttta aagaaatcaa cagtcttttt aatcgtttct tgaggcgtcg
     8761 ttgttggaaa ttgtattttt tctacaactt ttaattcctc atcaccaact gcacaaacaa
     8821 actttgtccc acctgcttca atactaccat ataactttgt catctttttt cctatctata
     8881 atctctatct caaagacaaa ggttgggata attatcccaa cctttaatcc tcacttaaca
     8941 tcaataatca tgtcacctgt agtaacaaca ccctctttta ccaatggtat aacatcagca
     9001 aaatcagctg tattagtcac aataatcata gctgtattat ccagacctgc ttctgcaatc
     9061 ttggctgaat caaaggtgcc aagaatatca ccttttttca ctttttggtc tgcctttact
     9121 ttagaagtaa accctgtgcc attcatggtg acagtatcaa tacctatatg aatcaaaatt
     9181 tctgcacctt tatctgattt gataccgtaa gcatgacctg tttcaaaaac aatttggaca
     9241 aaaccatcaa caggtgagta aattgtatta ccgcttggtt taatagcaag gccatctccc
     9301 ataatacctg atgaaaaaac tgggtcactt actgtatcaa gagcaataac ctgtccatca
     9361 agagggctaa caatcgtttc tgcccttaat tggctagttt gagtaattgt tggattgtca
     9421 tctgtttctt caaccaaagg actaacttca gctactactt tttcatcttt atcttcatat
     9481 ccaaacatat aagttaatac aaaagccaaa gccgtagttc caatcaccat aataaggtac
     9541 ttaacaattt gaccatttaa ataaagtaat gttcctggga taatggtaat accaaaacca
     9601 gttcctgcta atttcaatat tgaagctaac cagccacctg ctgctcctgc aattaatccc
     9661 ataataaatg gcttaccaaa acgtaagttt actccaaata tagccggttc tgtaatacca
     9721 agtccagctg aaagcgctgc cggaaaagca agggctttta attttttatt ttttgtttta
     9781 acaccaactg ccaaagtggc acctgcttgt gctgtcatag cagctgtaat aatagcatta
     9841 aatggatctt tcccatcagc agcaattaac tgagcttcaa gtaaattaaa gatatgatga
     9901 acacctgtaa caacgattat ctgatgaaca ccacctaaaa taagacctga taggccaaga
     9961 ggtaaattaa ggacaaattt tgtccctgct aaaacatagt tttcaactga atggaaaata
    10021 ggaccaatga caaaaagtgc taaaattgac ataactgtaa aagttaggaa cggcactaat
    10081 agtaaatcaa gaacatctgg gattttctta tgcaaccatt tttcaagttt agcaccaagt
    10141 aaacctacaa agaatgccgg caatactgaa ttttgataac caactactgg aataaagccg
    10201 aaaaatttta tgggatgggc atcacctgag gccactgccc aagcgtttgg aagggcagag
    10261 ttaaccatca tcaatcctaa tacgagaccg ataacaggat ttccaccaaa aactctaaaa
    10321 gctgaccaag aaatcaaggc aggaaaaaaa gcaaaggccg tgtcagtcaa aacgacagta
    10381 taggtataaa aattagaact tgaaaatgct tttgaagtcg tcccaaataa agctaaaact
    10441 gtatcattat taatcgcacc gcgaatcccc ataaataaac cagtagcaac aatggctggc
    10501 aacaatggaa caaaaacatc accgaaagta cgaatagctc gttggaaagc attcccttgt
    10561 ttagctgctt ctgccttttg atcagaggtt gacgaagttg gcaaaccttg agccactact
    10621 tcatcataaa tcttattaac agtaccagtt ccaaaaataa tttgatattg acctgaattg
    10681 aaaaatgctc cctgaacttt ttcgatattt tcaacagtat ttttatcaat gactgtctca
    10741 tcctttacca taacgcgaag acgagtggca cagtgtgcta cgctacgcac attactagca
    10801 ccaccaatcg cattaatgac ttgtttagca atttctgtat ttgaagccat ctataaatct
    10861 cctttgatat tttgaaagcg tttcatttta tttactctaa tattctagca cgtctcataa
    10921 atatatgtca agcgtttgac ataaaataat attttttgat attaagagtt gatttcaaaa
    10981 gagaaaacgt ttactatata gttattagat attaagacag tcaaggagta tcaaatgaat
    11041 ttacccactg aaatccgcta tcgtccttat gatgaatgga ctgaagaaga taaggaaaat
    11101 attgtaaaga atgtctcaaa atccccttgg agagcaactt atcacttaga agctaaaaca
    11161 gggcttttaa atgacccaaa tggcttctct tactttaatg gaaaattcca tcttttttac
    11221 caaaattggc cctttggagc tgcacatggt ttaaaacaat gggtacatac agaatcagat
    11281 gatttagttc atttcaaaga aacaggaatc aagcttaaac ctgaccatgt caatgatagc
    11341 catggtgcat actccggttc tgcattagcc attgacgata agctttttct tttttatact
    11401 ggtaatgtcc gtgacatgaa atggaaccgt gacccccgcc aaattggagc atggatgaca
    11461 agtgatggca agattacaaa atttgataag gtccttattt cacaaccaaa tgatgtcact
    11521 gagcattttc gtgaccctca gattttcaat tatgataatc aattctatgc tgttattggg
    11581 gcgcaaaata gtaaaaaatg cggattcatt aaattatata aagcacttaa taatgacatc
    11641 catcattggg agtttgtggg agatttagat tttggtggta ctggatctga gtatatgatt
    11701 gaatgtccaa atattatttt tgtcaaagga aaacctgtat tactttatag tcctcaagga
    11761 ctagataaaa acgaattgga ctaccaaaac atttatccaa atacctacaa aatcggacaa
    11821 tatttcgatg ctaactcatc taagatagtt gaaccatccc ctatctataa tttagattat
    11881 ggttttgaag cctatgctac tcaaggattt aacacttctg atggtcgtgc ctttatcgtg
    11941 agttggatag gcttgccaga tattgattat cctagcgatc aatttgatta tcaaggagct
    12001 atgagtttag ttaaagagtt aagtataaaa aatgggaacc tttatcaata tcccgttcct
    12061 gctatgaaaa atcttcgtca acatcaagca gaatttaaaa cacaacttca aactaacaac
    12121 acctatgaat tagaattact ggttcctaga aatgatcttt cttcatttgt tctctttgct
    12181 aatccgaaag gacaaggact atccattact attgatactg caaaggggaa agttatcgtt
    12241 gaccgctcac aagccggaca gcagtacgcc acagaatttg gtactagtcg ccagtgtaac
    12301 attccaaaag atgctactag tgtcaatgtt tttatcgata agtccatctt tgaaatcttt
    12361 ataaataaag gagaaaaagt atttacggga cgagtctttc cagatgcaga acaatcaggg
    12421 attcaactaa aagaaggaca cgttcatggt aaatattttg aattaaaata ttaatatggt
    12481 tgcaaaatta acagatgttg ctgcacttgc cggtgttagc ccaacgactg tatcacgcgt
    12541 tattaacaaa aaaggctatt tatctcaaaa aacagtcacc aaagtcaatg aagccatgag
    12601 aacccttggt tataaaccta ataatttagc acgtagctta cagggaaagt ccgctaaatt
    12661 aattggactc attttcccca acatccgtaa cattttttac gctgaattaa ttgaacacct
    12721 tgaaattgag ttgtttaagc atggttataa aactatctta tgtaatagtg aaaaggatcc
    12781 tatcaaagaa aaagaatacc tcgaaatgct aggagcaaat caagtagacg gtatcatttc
    12841 aagtagtcac aacctaggaa ttgatgatta cgagaaagtt gaggctccca tagtagcatt
    12901 cgatagaaat ttagctccac acatccctat cgtctcatct gataatttct ttggaggaaa
    12961 aatggctgct caaaccctta aaaaacatgg ctgtcaaaag atgattatga ttacagggaa
    13021 tgataattca gattctccta ctggacttag gcggctaggt ttttcctatg aatcaaagga
    13081 atccaaagtt atcacagtca caaatgggct atctaatatg agacgtgaga tggaattaaa
    13141 atcaattatc tcgactcaca aaccagatgg tatttttaca tcagacgact taactgcttt
    13201 attagtaatc aaacttatca gccaactagg gttgtccatt ccagaagata tcaaagtgat
    13261 tggatatgat ggtacaagtt tcattcaaga ttacgtccca catctaacca ctattaaaca
    13321 gcctattcga gaaattgcgc aattgatggt tgaaatactc ctagctaaga ttgaggggca
    13381 aaagacaaat aaggattata ttttaccagt cagtctcatc cctggtagta gcgtttagga
    13441 aatcatattt atgaaataat ttttagtacc gatagttatt tttcaaagca aattaaaaat
    13501 gtggaagaag attcttccac atttttaatt ttcatttgtt ataaattgac ttaataaacc
    13561 attgacaaat tttgctgacg tctcatctga atatttttta gcaatctcga taatctcatt
    13621 tagagctaca cgatcaggcg tttcatcaaa atatttaatt tcatagaggc ctaagcgtaa
    13681 gagagattta tcaaccaatg ttagacgttc taagctccat cctgatttta agtgactaga
    13741 aatcaaagta tctaattcat ctttgtgatc tacaacacca ttaacaagat taagtaaaaa
    13801 gattggaacc tctaaaactt tgtcttcaga tactgtttta tcataaccat aagcaaagtg
    13861 agccgcatca ataaactctc caccagtttc taagctaaat agtgtttgaa aagcacgttc
    13921 acgtaaatca cgtcttgaat ctttaaatac actagtcatc taagaaatcc tcatcaaaca
    13981 atgacttcaa gtctggtttt ggtgttttct ctggaacaat accttcaaca tggatattga
    14041 ccgaagaaat ttttacttct gccatgtcat atacagctgt tttgacagct tgttgaatag
    14101 caattgaaac tgctggtaca tttacaccat actgcaagta aacataaata tctgcagcta
    14161 ctgtatcatc ttcttcattt ttaaggtaga cacctctacc tagagatttc ttactaaggc
    14221 tatccgttac cgctttgtta cgtaacgaat gaacaccatc aactttagtt gcagcaatac
    14281 ctgtaattac ctcaagtaca cgtggtgaaa tgacaatttc tccaagattt tcagttgtca
    14341 tagcttatcc tttcttttag tgtttactaa ggtttttcct taatagagta aattcatcat
    14401 gaatctactt aacacttttt tgagagtcat taggcgcgtg aaacgtaagt tccttctgct
    14461 gtattgatta ctaacttttg tccagcttca ataaagtctg gaacgttaac aacaagtcct
    14521 gtttccatag ttgcaggttt tcctgaacca gttacagtag cacctttgat tgatggttga
    14581 gtttcagcaa ctgtcaattc aacagttgtt ggaatttgaa caccaataac ttctgttcca
    14641 tagaattgaa tttttacttc tgagttttca agaatgtaga gtaattcatt ttcaatgtta
    14701 acagttggaa tttcatattg atcgtaagtt tcattattca tgaagtaagc tgtatcatcc
    14761 attttataaa ggtattgagc aggtacagtt tcaataatag cttgctcaaa tttttcttca
    14821 ggacggtaac ttgtatcaaa agttgaacca gtacgaacat cacgcaattt catacgcatg
    14881 attgtgttac ctttacctgg tttgtggtgg ctagcttcaa gaacacggat taatttccca
    14941 tcagccgttt caaaagtcat gcctgcttta agcttacttg cttcaatcat ttattaatac
    15001 ctctttgtaa aaattattta tcaactcatt ttatcacaaa taacctgctt tctcaaatag
    15061 tttattgatt tggtatgtgt ctaatttctt gataaataaa taaaatcacc aagagggcgt
    15121 aaataagact gaagcacttg acactttcct cttacttcag ttttgataat attgcctttt
    15181 gacgatacaa tatttttaaa ctgattaagc aagtagcatt tgcttcaatc ggttggcgag
    15241 aggtgtttgc acacctttcg ccttttcttc gtgtttcttt aataagatta tgtcacaata
    15301 gaatattatt aaagaaaaaa tcgtaaattt attgcctgac atcacttatt tttcaaggtt
    15361 tcagcaagtt ttataataat tagtcataaa atgatcttct agatttggga gatttttcat
    15421 ttctccgtag tgaaggtaaa ttatattctt ttaatgtata tttaaaatga tttcctaggt
    15481 ttcttaatcc ttcatttttg attgcttcat attgtgctct ccattttccg aaaaataaat
    15541 ctcttaaatt gccttcatga cttaaaagga ttgtttagct cataacttcc gaaccatttc
    15601 tactccaata cattcctcta tttttcagta gctgccttct aaatctctga aaagaagaca
    15661 gtttactttc tgattcaatt aatgattctt ctgtatctaa taccgtttta agcgattttt
    15721 tacttctttt cttgattact ttaatggcta gattctttaa ctctgatggt aagttagaaa
    15781 aattcacttt gataacccga ttaatatgaa acgtatccca aaaatgatag tgttcattgg
    15841 tttaaaccca ctgactaatt ctttaaaata ttgtgatgaa tacccaagtc cacccgtctg
    15901 aattagtaat aaaagtagtc tcaggggtgt tctcataatg attatacaac tgatcaataa
    15961 gttgttcttt ttttaaatgt atttaattgt ttactagagt atagagaaag atataggtct
    16021 tttagttaat aattgttcct tcatgtcttg ctgca
//
LOCUS       DBJYSH010000043        16000 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.043, whole genome shotgun sequence.
ACCESSION   DBJYSH010000043 DBJYSH010000000
VERSION     DBJYSH010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 16000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16000
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.043"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            119..1138
                     /locus_tag="ACUBV3_001051"
     CDS             119..1138
                     /locus_tag="ACUBV3_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3847558.1"
                     /translation="MKINKLLSDRLEACYSVCRLQNQDYDFLLVASEVDQACFAYDLN
                     SDLAKIVVWEDVGGTMSIIPIPGTMDFLATQKFYPGFKAKDCQVVYASFQGDHWDICN
                     AFDFPYLHRFDLIKGKENQLYFIGCTIANSKAHTEDWSDKGRIFVARFNTQNHTLEGL
                     QQLPQTLLKNHGYYAIQDEGYSLITSVEGVFKLTYPEFSTTGDWQLERLFDEETSDVV
                     KVDINQDGKDEYVIIQGFHGDRLRIFTEDFGRELFHYPEKTPFGHAIWSGRLLNQTCF
                     VFGWRSEKAELRLFHFVDGHLVSELVDAKAASSNVLAFEKDGKAYLFSANNGRGEVAL
                     YQLVK"
     gene            1157..2113
                     /locus_tag="ACUBV3_001052"
     CDS             1157..2113
                     /locus_tag="ACUBV3_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017766789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HHU3847559.1"
                     /translation="MDKKKILVTGTVPKEGLRKLMDRFDVTYSEDRPFSRDYVLEHLS
                     EYDGWLLMGQKGDKEMIDAGENLQIISLNAVGFDHVDTAYAKEKGIIVSNSPQAVRVP
                     TAEMTFALILAASKRLAFYDSIVRSGEWIDPSEQRYQGLTLQGSTLGIYGMGRIGLTV
                     ANFAKAFGMTVVYNDVYRLPEDKEKELGVTYLEFDQLIKTADVITIHAPALPSTIHKF
                     NKDVFAKMKNRSYLINAARGPIVSEEALIEALKEGEIAGAGLDVFENEPQVSEGLRSL
                     DNVIMSPHAGTGTIEGRRTLAEEAADNIIAFFDGKPQNIVNK"
     gene            2211..3662
                     /locus_tag="ACUBV3_001053"
     CDS             2211..3662
                     /locus_tag="ACUBV3_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000888340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HHU3847560.1"
                     /translation="MLEALHKFFEVFGAATVVPAMIFIVSLFLKVNPRRAFFSALRAG
                     VGLTGFGWIISAFAPMVTKYIQQMVKTTGLHLPIVDIGWQAGSLTAFSSEIGLSFFVF
                     GLLIELGLFLLGITRVFVPSNLWNNFGYMIWGTMAYAATGNFILSFAFMVFVLLYSLV
                     MSEVLADRWSEYYGVKNATINSIHNIETLIPALILDPLWNLLGVNKVKLNPESLKTKL
                     GIFGEPMTLGFILGVIIGVLGSLRNLASIDTWGGILGFAVALAAVMTIFPLITGVFAS
                     AFAPLAEAVERNKKKESQAEQGALDKKRWFIAVDDGVGFGEPATIIAGLILVPIMVVI
                     SLILPGNEALPVVDLIAIPFMIEAMIAVSKGNILKAILNGIIWFSLGLYAASALGPIY
                     TEAVKHFGTALPAGVTLIMSFNLLAHPLTALVFFAWISGNPLYIGVTVVIYLAFLFLL
                     RTHRESIYAYLKTMADKNSGYEGSCEKIFIAED"
     gene            3684..4673
                     /locus_tag="ACUBV3_001054"
     CDS             3684..4673
                     /locus_tag="ACUBV3_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HHU3847561.1"
                     /translation="MKKIDGHAHIVDTIAGFNGKGRLNALGNGYAIWDDGDLIKLIPD
                     EYGDKAFTAEAFLKYMDANGVDKAVILQGHLNGYQNYYTHLAIKRYPERFTGAFSVDP
                     FADNAMQIVKRHVEVLGFRAIKFEISQGGGIHGYRGQKTPFRLDTDPHVSRILTYLLD
                     YPGFVVTVDYGNWDQISHQPDAIANLARLYTSLDFVVCHLSFPHVEHSNRLRAELNMW
                     KNFNNIYTDISAIQDIDAPDSFPFPKSEQNVRIAKEVLGAKRIIWGTDSPWSATFNTY
                     EELATWLENVDIFSQEELEDVMYNNAERVYFKEEHVKANRAGIDDTTKELNLY"
     gene            4739..6256
                     /locus_tag="ACUBV3_001055"
     CDS             4739..6256
                     /locus_tag="ACUBV3_001055"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HHU3847562.1"
                     /translation="MTYYLSIDYGGTNTKALIFDKLGHQIAVSSFETLKNETQSGHRQ
                     VNLVKTWNAITSAIREVIQISKLSPEQISAVACIGHGKGLYLLDNKLEPLEQGILSTD
                     NRAKDLAQYFESKLDNIWELTRQHIFPSQSPVILRWLKDYQPETYKSIGAVLSAKDFI
                     RYKLTGKVQQEYGDASGNHWINFQTGTYDPAILDFFGIREIENSLPELIDSADLVPGG
                     ISSQAAKETGLVEGTPVVGGLFDIDACALGSGVLESDTFSVISGTWNINTYPSLKPAK
                     QDSGLMTSYFPDRRYLLEASSPTSAGNLNFMLKMLMHQEIDNAKSSGGSIYDNLEEFL
                     THTDATHHGLIFFPFLYGSNTSQDASACFFGLTTKSTKSQMIRAVYEGIAFAHKQHIT
                     DLIKSRGSVPKIIRFSGGATNSPAWMQMFSDILNFPIETVEGTELGGLGGAILARHAL
                     DKISLKEAVQDMVRVKAIYKPQLSEVKGYKKKYHAYQKLLETLDPIWSELGHLNK"
     gene            6276..7037
                     /locus_tag="ACUBV3_001056"
     CDS             6276..7037
                     /locus_tag="ACUBV3_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HHU3847563.1"
                     /translation="MKQEDIIINSIVFKKQLDKGFTQVQLIKLVKDLGFSRFEIRQEL
                     LQDPDRELPALKAEADFYDINLYYSANEDLIKGGKVNPYLNKGLKEASQLGAPFIKLN
                     VGQTRNLSKEELEPLKEILKSQTIGIKVENNQDPKAATVENCQYFMTLVKELQIPISF
                     VFDTANWAFINQDLYQAVNSLACDTTYLHCKNFLQVAGKPHLSKSLFEGEIDLTDLLK
                     SFSNCEYLALEYPTELEVLKRDVQRLISISNSQNF"
     gene            7103..8782
                     /locus_tag="ACUBV3_001057"
     CDS             7103..8782
                     /locus_tag="ACUBV3_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000584682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HHU3847564.1"
                     /translation="MIILDKKSYDLLFYLLKLEEPETVMAIANALNQSRRKVYYHLEK
                     INDALPSDVPQIVSYPRVGILLTEKQKAACRLLLDEVTDYSYVMKSSERLQLSLVSIV
                     VAKDRITIDRLMQLNDVSRNTILNDLNELRSELAEKEYNLQLQSTKCRGYFLDGHPLS
                     IIQYLYKLLDDIYHNGSSSFIDLFNHKLSQAFGASTYFSKEVLDYFHHYLFISQRSLG
                     KKINSQDGQFMIQILPFILMAYRKMRLSPEVQTSLNSDFSLVWQRKEYEIAKELADEL
                     EENFQLSLDEIEVGLVAMLMLSFRKDRDNHLESQDYDDMRATLTSFLKELEERYHLHF
                     VHKKDLLRQLLTHCKALLYRKRYGIFSVNPLTEHIKDKYEELFAITSSSVKLLEKAWQ
                     IKLTDDDVAYLTIHLGGELRNSQQSPNKLKLVIVSDEGIAIQKLLLKQCQRYLTNSDI
                     EAVFTTEQYQSVSDLMHVDMVVSTSDALESRFPMLVVHPVLTDDDIIRLIRFSKKGNC
                     ANSNQFTNELEKTIAQYVKEDSERYVLKSKIEKLIHQELLQDVLPLQSTVC"
     gene            9099..10190
                     /gene="ulaG"
                     /locus_tag="ACUBV3_001058"
     CDS             9099..10190
                     /gene="ulaG"
                     /locus_tag="ACUBV3_001058"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HHU3847565.1"
                     /translation="MPNVKDITRESWILSTFPEWGTWLNEEIEEEVVADGNFAMWWLG
                     NCGVWIKTPGGANVVMDLWSNRGKSTKKVKDMVRGHQMANMAGVRKLQPNLRAQPMVI
                     DPFAINELDYYLVSHFHSDHIDINTAAAIINNPNLDHVKFVGPYECGEIWKKWGVPEE
                     RIIVIKPGESFEFKDIKVTAVESFDRTCLVTLPVDGAEEHDGELAGLAVTDEEMARKA
                     VNYIFETPGGTIYHGADSHFSNYFAKHGKDYKIDVAINNYGDNPVGIQDKMTSIDLLR
                     MAENLRAKVIIPVHYDIWSNFMASTDEILQLWKMRKERLQYDFHPFIWEVGGKYTYPQ
                     DKDRIEYHHPRGFDDCFEQESNIQFKALL"
     gene            10280..12658
                     /locus_tag="ACUBV3_001059"
     CDS             10280..12658
                     /locus_tag="ACUBV3_001059"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HHU3847566.1"
                     /translation="MSEFDTKSYLEKLDAWWRAANYISAAQMYLKDNPLLRRELVEND
                     LKVHPIGHWGTVPGQNFIYAHLNRAINKYDLDMFYIEGPGHGGQVMVSNSYLDGSYTE
                     LNPNIEQTEDGFKQLCKIFSFPGGIASHAAPETPGSIHEGGELGYALSHATGAILDNP
                     DVIAATVIGDGEGETGPLMAGWLSNTFINPVNDGAVLPIFYLNGGKIHNPTIFERKTD
                     EELSQFFEGLGWKPIFADVVELSEDHAAAHALFAEKLDQAIQEIKTIQSEARQKPAEE
                     AIQAKFPVLVARIPKGWTGPKAWEGTPIEGGFRAHQVPIPVDAHHMEHVDSLLSWLQS
                     YRPEELFDESGKIVDEIAAISPKGDRRMSMNPITNAGIVKAMDTADWKKFALDINVPG
                     QIMAQDMIEFGKYAADLVDANPDNFRIFGPDETKSNRLQEVFTRTSRQWLGRRKPDYD
                     EALSPAGRVIDSQLSEHQAEGFLEGYVLTGRHGFFASYESFLRVVDSMVTQHFKWLRK
                     SKTHTTWRKNYPALNLIAASTVFQQDHNGYTHQDPGILTHLAEKTPEYIREYLPADTN
                     SLLAVMDKAFKAEDKINLIVTSKHPRPQFYSIAEAEELVAEGYKVIDWASNVSLNQEP
                     DVVFAAAGTEPNLEALAAISILHKAFPELKIRFVNVLDILKLRHPSQDARGLSDEEFD
                     KVFTTDKPVIFAFHSYEDMIRDIFFSRHNHNLHTHGYRENGDITTPFDMRVMSELDRF
                     HLAQDAALASLGNEAQAFSDEMNQMVAYHKDYIREHGDDIPEVQNWKWENIK"
     gene            13035..14258
                     /locus_tag="ACUBV3_001060"
     CDS             13035..14258
                     /locus_tag="ACUBV3_001060"
                     /EC_number="7.6.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015697 - quaternary ammonium group
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary amine ABC transporter ATP-binding
                     protein"
                     /protein_id="HHU3847567.1"
                     /translation="MTNILEVKNLTKIFGKKQKAALEMVKQGKSKTEILEKTGATVGV
                     YDASFEIKEGEIFVIMGLSGSGKSTLVRMLNRLIDPSSGNIYLDGKDIAKMNVEDLRN
                     IRRHDINMVFQNFGLFPHRTILENTEFGLEMRGVSKEERTMLAEKALDNAGLLPFKDQ
                     YPSQLSGGMQQRVGLARALANSPKILLMDEAFSALDPLIRREMQDELLDLQDTNKQTI
                     IFISHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDFVREFVEDVDRSKVLTA
                     QNIMIKPLTTVLEIDGPQVALTRMHREEVSMLMATNRRRQLLGSLTADAAIEARKKGL
                     PLSEVIDKDVVTVSKDTVITDIMPLIYDSSAPIAVTDDNDRLLGVIIRGRVIEALANV
                     QDETVVESPKETVEA"
     gene            14277..>16000
                     /locus_tag="ACUBV3_001061"
     CDS             14277..>16000
                     /locus_tag="ACUBV3_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HHU3847568.1"
                     /translation="MENLLQHKLPVAPFVESTTNWITKTFSGLFDFIQTIGNALMDWM
                     TKTLLFINPLLFIVLITVAVFFLAKKKWQLPTFTFIGLLFIYNQGLWEQLINTFNLVL
                     VASLISIIIGVPLGIWMAKSDKVKQVVNPILDFMQTMPAFVYLIPAVAFFGIGMVPGV
                     FASVVFALPPTVRFTNLAIREIPLELIEASDSFGSTAKQKLFKVELPLAKNTIMAGIN
                     QTMMLALSMVVTGSMIGAPGLGREVLSALQHADIGTGFVSGLSLVILAIVLDRVSQFF
                     NSKPGEKQAKTSKVKKWVGLGALVLFILAALGRIVVNMTSGNEAKGQKVKIAYVQWDS
                     EVASTNVIAEVLKSKGYDVELTPLDNAVMWQTVANGNADFTTSAWLPKTHGQYFNKYK
                     NSLDDLGPHVENVKIGLVVPKYMNVNSIEELSNQADKQITGIEPGAGIMKSAKQSLKD
                     YPNLSSWKLLSASTGAMTTTLGKAIKNKDQVVITGWSPHWMFAKYDLKYLKDPKKSFG
                     GEEHINTIARKNLKKDMPKVYKIIDKFKWTKEDMESIMLDMDKGMEPAKAAQKWIKNH
                     KKEVSEWT"
BASE COUNT         5080 a         2663 c         3216 g         5041 t
ORIGIN      
        1 aagaataggt aagcttttaa cgaaccatct ctaactctct taactcaaat gtttttattt
       61 ttttgcacca ttagttaaac ggttaactca agttttatct tcaccaaagg aggatagaat
      121 gaagattaat aaattattgt cagacagact tgaagcttgt tattcagttt gtcgtttgca
      181 aaatcaagac tatgattttt tattagtagc ttcagaggtt gaccaagcct gctttgctta
      241 tgatttgaat agcgatttag caaaaattgt tgtttgggaa gatgtgggag gtaccatgag
      301 tattattcca attcccggaa ccatggattt tttggcaacc cagaaatttt acccagggtt
      361 taaggccaaa gattgtcagg ttgtttatgc aagttttcaa ggagatcatt gggacatttg
      421 taacgcattt gattttccgt atttacatcg ctttgatctc attaaaggta aagaaaatca
      481 actttacttt ataggttgta caattgctaa cagtaaagcc cacactgagg attggagtga
      541 taaaggccga atttttgttg ctcgttttaa tactcaaaac catacattgg aaggattgca
      601 acaattgcct caaactttat taaaaaatca tggatactat gccattcagg atgaaggata
      661 ttcattgatt acttcagtag aaggggtatt caaactcact tatccagaat tttctactac
      721 aggcgactgg caattagaac ggcttttcga tgaggagaca agcgatgtgg tgaaagtgga
      781 tattaatcag gatggcaagg atgagtatgt gattatccaa ggttttcatg gagatcgttt
      841 acgcatcttc actgaagatt tcggtcgaga attattccat tatccagaaa aaacaccatt
      901 tggtcatgct atttggagtg gtcgtttact taatcagact tgttttgtat tcgggtggcg
      961 atcagaaaaa gcagaattaa ggctttttca ctttgtagat gggcacttgg tttcagaatt
     1021 agtagatgca aaagcagctt ctagtaatgt cttagctttt gaaaaagatg gaaaagctta
     1081 tcttttctca gccaataacg gacgtggcga agttgctctt tatcaattag taaaataaat
     1141 ttaagaagga gacaatatgg acaagaaaaa aatcttagta acgggtactg tgcctaaaga
     1201 aggtctaaga aagcttatgg accgatttga tgttacttat tcagaagatc gcccattttc
     1261 acgtgactat gtgttagagc atttatctga atatgacgga tggttactca tgggacaaaa
     1321 aggcgataaa gagatgattg atgcaggtga aaacttacaa attatttctt taaatgcggt
     1381 tggttttgat catgtagata ctgcgtatgc taaagaaaaa ggaattatag tctctaattc
     1441 ccctcaagca gtacgagttc cgacagcgga aatgactttt gctttaattt tagctgccag
     1501 taagcgcctt gcattttatg atagtattgt aagaagtgga gagtggattg atccgagcga
     1561 acaacgttat caaggtttga ctttgcaagg ttcaacatta ggtatttacg gaatgggacg
     1621 catcggtttg acagtcgcaa attttgctaa agcatttggg atgactgttg tttacaatga
     1681 tgtttatcga cttcctgaag ataaggaaaa agagcttggt gtaacctatc tcgaatttga
     1741 tcagcttatt aaaacagctg atgtcataac catccacgcc ccagcattac catcaacaat
     1801 ccacaaattc aataaagatg tttttgctaa gatgaaaaat cgaagctact taatcaacgc
     1861 tgctagaggc ccaatcgttt ctgaagaagc acttattgaa gcactgaaag aaggtgaaat
     1921 cgcgggtgcg ggattagatg tttttgagaa tgaaccacag gtatctgaag gattgagaag
     1981 ccttgataat gttatcatga gtccacatgc tgggacaggt actattgagg gacgtagaac
     2041 tttggctgaa gaagcagctg acaacatcat tgccttcttt gatggcaagc cacaaaatat
     2101 tgtcaataaa taattggcta gtgagcgcag agccatcttt tgaaaaaatg ggacctccca
     2161 tttttttacg ggtaaatgta agcgcttaat aatttaataa aggtgaactt atgctagaag
     2221 ctttacataa attctttgaa gtctttggtg cagcaactgt tgtacccgct atgattttta
     2281 ttgtcagcct ttttctaaag gtcaatccaa ggcgagcttt ctttagcgcc ttacgagctg
     2341 gtgttggact cacaggcttt ggttggatta tatcagcttt tgctcctatg gtgactaagt
     2401 atatccagca gatggttaaa actactggtc tccatttacc aattgtagat attggttggc
     2461 aggctggttc tctcaccgcc ttttcttctg agattggcct ctctttcttt gtttttggct
     2521 tgctaattga attggggctt ttcttgcttg ggattacccg agtatttgtc ccatctaatc
     2581 tttggaataa ctttggctat atgatttggg gtacaatggc ctatgcagca acaggtaatt
     2641 ttatattgtc tttcgcattt atggtttttg tcttactcta ttcattagtg atgtcagaag
     2701 tattggcaga tcgatggtcg gagtattacg gtgttaaaaa tgcaacgatc aactcgattc
     2761 ataatattga aacccttatt ccagctctta ttcttgatcc tctttggaac ctacttggtg
     2821 tcaataaggt aaagttgaac ccagaatcat taaagactaa gttagggata tttggtgagc
     2881 caatgacact tggttttata cttggtgtga ttattggtgt attaggtagt ttacgtaacc
     2941 ttgcaagtat tgatacttgg ggaggtatac ttggttttgc agttgctctt gcagcggtta
     3001 tgactatttt tcctttgatt acgggggtat ttgctagtgc ttttgctcct cttgctgaag
     3061 cagttgaacg caataagaaa aaagaaagtc aagctgagca aggagcactg gataaaaaac
     3121 gttggtttat tgcagttgat gacggtgttg gttttggtga accagcaact attatcgcag
     3181 gtctcatttt agtaccaata atggttgtta tttccttgat tttacctgga aatgaagctt
     3241 taccagtcgt tgaccttatt gctatccctt ttatgattga agctatgata gctgtgtcaa
     3301 aaggaaatat tcttaaagct attttgaatg gaatcatatg gttttcacta ggtctttatg
     3361 ctgctagtgc cctaggtcca atctacacag aagctgttaa gcattttgga actgctttac
     3421 cagcaggggt aactttgatt atgagcttta atctcctagc tcatcctttg acagctctag
     3481 tcttctttgc ttggatatct gggaacccac tctatattgg tgtgacagtt gttatctatc
     3541 ttgctttctt gttcttgctt cgtactcatc gtgaatctat ttacgcatac cttaaaacta
     3601 tggctgataa gaatagcgga tatgaaggta gttgtgaaaa aatctttatt gctgaagatt
     3661 aaaaatctgt tggaggaata ctcatgaaaa aaattgatgg acatgctcat attgttgata
     3721 caattgcagg atttaatggt aaaggacgtc ttaatgccct tggaaacggt tatgctattt
     3781 gggatgatgg tgatttaatt aagctcatcc cagacgaata cggtgacaaa gcatttacag
     3841 ctgaagcttt tttgaagtat atggatgcta atggtgtaga taaagcagtc attttgcaag
     3901 gtcatcttaa tggttatcaa aattattata cgcatttagc tattaaacgt tatccagaac
     3961 gttttacagg cgcattctct gttgatccat ttgcagataa cgcaatgcaa attgtaaaac
     4021 gtcatgttga agtccttggt tttcgagcta ttaaatttga aattagtcaa ggagggggta
     4081 tccatggata ccgtggacaa aaaacgcctt tccgtcttga tacagaccca catgtgagtc
     4141 gtattttgac ttatctgtta gactatccag gatttgtagt tacagttgat tatggcaatt
     4201 gggatcaaat tagtcaccaa cctgatgcta ttgctaattt agctcgcctt tatacgagtc
     4261 tcgattttgt tgtttgtcac ctctcttttc cacatgttga gcattcaaat cgattgcgtg
     4321 ctgaacttaa tatgtggaaa aacttcaaca atatctatac tgatatttca gctattcaag
     4381 atattgatgc cccagatagt tttccttttc caaaatcaga acaaaatgta cgtattgcta
     4441 aagaagttct tggggctaag cgtatcattt ggggaactga ttctccatgg tcagcgacct
     4501 tcaatactta cgaagaatta gctacttggc tagagaatgt tgatattttc agtcaagagg
     4561 aactagaaga tgttatgtac aataatgctg agcgtgttta ctttaaagaa gaacatgtaa
     4621 aggctaacag agcaggtata gatgatacaa ccaaagagct caatctctat tagaagagtt
     4681 ttcaaggtac aagagtttct tgtacctcct ttttaaatca acaatgagga gacaaattat
     4741 gacttattac cttagtattg actacggcgg tacaaatacc aaggcgctta tttttgacaa
     4801 attaggacac caaatcgctg tttcgagttt tgaaacttta aaaaatgaga ctcaatctgg
     4861 tcatcgtcaa gtaaaccttg ttaaaacgtg gaatgctata acttctgcta ttagagaggt
     4921 tattcaaatc tcaaaactca gccctgagca gattagtgca gtagcatgta ttggacatgg
     4981 gaaaggtctt tatctgctag ataataagtt ggagccactt gaacaaggaa ttttgtctac
     5041 agataatcgt gccaaagatt tggcgcaata tttcgaatct aaacttgata atatttggga
     5101 gttgactcgg caacatattt tcccttcaca aagtccagtt attttacgtt ggcttaaaga
     5161 ttatcagccc gaaacctata aatcaatagg ggcggtcctt tctgcaaagg actttattcg
     5221 gtataagctt acaggaaaag tacagcaaga atatggtgac gcttcaggta atcattggat
     5281 aaatttccaa acaggaactt atgatccagc tattttagat ttttttggca ttagagagat
     5341 agaaaactca cttcctgaac ttatagatag tgcagattta gttcctgggg gaattagttc
     5401 tcaagcagca aaagagactg gtcttgtaga agggaccccc gttgttggag ggctctttga
     5461 catcgatgct tgtgctcttg gatcaggtgt tttagagtca gatactttta gtgttatttc
     5521 gggaacttgg aatattaata catatccaag tttaaaacca gcaaagcaag atagtggtct
     5581 tatgacttcc tattttccag atcgtcgtta tctcttagag gcaagtagcc ctacttctgc
     5641 agggaatctt aattttatgt taaaaatgct catgcatcaa gaaattgata acgctaaatc
     5701 tagtggaggt tctatctatg ataatttaga agaatttctc actcatactg atgctacaca
     5761 tcatggactt attttctttc cgtttcttta cggtagtaac acatcacaag atgctagcgc
     5821 ttgctttttt gggctaacaa ctaaatcgac gaaatctcag atgatacgtg cggtatatga
     5881 aggtattgcg tttgcacata agcagcatat cactgattta ataaaaagta ggggcagtgt
     5941 gccaaaaata attcgtttct ctggcggagc taccaactca ccagcatgga tgcaaatgtt
     6001 ttctgatatc ttaaactttc ctattgaaac agtagaaggc acagaattag gagggttagg
     6061 aggagctatt ttagcacgtc atgctttaga taagatttcg ttaaaggaag cagtccaaga
     6121 tatggttcgt gtaaaagcta tttataaacc tcaattatcc gaagtaaagg ggtacaaaaa
     6181 aaaatatcac gcttaccaaa aattattaga aacactggat cctatttggt cggaactcgg
     6241 tcatctgaat aagtagggat atcaaaggag ttgttatgaa acaagaagat attattatca
     6301 atagtattgt attcaaaaaa cagttagaca aaggttttac acaagttcag ctcattaagt
     6361 tagtaaagga tttaggattt tcacgatttg agatacgtca agaattgctt caagatccag
     6421 atagagagct acccgcttta aaagcagagg ctgactttta tgatatcaat ctttactata
     6481 gtgctaatga agaccttatc aaaggaggga aagttaatcc ttatcttaat aaaggcctta
     6541 aagaagcatc acagttagga gctcctttca ttaagctaaa tgttggacaa accagaaact
     6601 taagtaaaga agagttagaa cctcttaaag agatattgaa aagccaaact attggtataa
     6661 aggtggaaaa taatcaagac cctaaagcag ctacagtgga gaattgccaa tattttatga
     6721 ctctagtgaa agaattacaa ataccaataa gttttgtttt tgatactgct aattgggcct
     6781 ttatcaacca agatctatac caagcggtaa atagcttagc ttgtgatacc acctaccttc
     6841 actgtaaaaa ttttttacag gtagctggaa agcctcacct ctcgaagagc ctcttcgaag
     6901 gagagattga tctgactgat ttgttaaaga gtttctctaa ttgtgagtat ctagctctag
     6961 aataccccac agaattggag gtattaaaaa gggatgttca acgtttaatc tctatcagca
     7021 atagtcaaaa cttttaatga aaaggcttta aaaagatgtt ataataagtc tgtatgaatt
     7081 cgagtagaaa agaggtatat tcttgattat tctcgataaa aaaagttatg acctcctctt
     7141 ttacctattg aaattagagg aacctgaaac agttatggca attgccaacg cacttaatca
     7201 gtctaggcgt aaagtgtatt atcacttaga gaagataaac gatgcactgc ctagcgatgt
     7261 gcctcagatt gttagttatc cacgtgtagg aatcttgcta accgaaaaac aaaaagcagc
     7321 ctgtcgtctt ttattagatg aagtaactga ttatagttac gtcatgaaaa gtagtgagag
     7381 gttgcagctg tctttagtat ctatcgtggt agctaaggac cgcataacga ttgataggtt
     7441 gatgcaacta aacgatgttt ctcgaaatac tatcttaaac gatttaaacg aattaagaag
     7501 tgagttagca gagaaagaat ataatttaca gttacaatca acaaaatgtc gcggttattt
     7561 tttagatggt cacccattgt ccattatcca gtacttatat aagctcttag atgatatcta
     7621 ccataatgga agtagtagtt ttatagacct ttttaatcat aaactgtctc aagcttttgg
     7681 tgccagcaca tatttttcta aagaggttct tgattatttt caccattatc tcttcatttc
     7741 tcaacgaagt ctaggtaaga aaatcaacag tcaagatggt cagtttatga ttcagatttt
     7801 gccttttata ctaatggctt atcgtaagat gcgattaagt cctgaagtac agacctctct
     7861 taatagtgat tttagcttgg tttggcaacg taaggaatat gagattgcta aagagttggc
     7921 tgatgagctg gaagaaaatt ttcagttatc actggatgag attgaagtgg gactagtggc
     7981 catgcttatg cttagtttcc gcaaggaccg tgacaatcat ttagagagcc aggattatga
     8041 tgatatgcga gctactctaa ccagtttttt gaaagaattg gaagaacgat atcaccttca
     8101 ctttgttcat aaaaaggact tactaagaca acttctaact cactgcaagg cactcttata
     8161 tcgtaaacgt tatggtattt tttctgttaa tcctttaaca gagcatatta aagacaaata
     8221 tgaagaactt tttgccataa cctcgtcttc tgtaaagctt ttagagaaag cttggcaaat
     8281 caaattgacc gatgatgatg tagcatatct aacgattcat ttaggagggg aacttcgtaa
     8341 tagtcaacaa tctcctaata aacttaagtt agttattgta tctgatgaag gaatagcgat
     8401 tcagaaactt cttttaaagc aatgtcaacg ctacttaaca aacagtgata tagaagctgt
     8461 ttttacaacc gaacagtacc aaagtgtgag tgatcttatg catgtagata tggttgtctc
     8521 tactagtgac gctttagaat ctcgttttcc gatgttagta gttcaccctg ttttgacaga
     8581 tgatgatatt attcgcttga ttcgcttttc aaaaaaaggt aactgtgcaa atagtaatca
     8641 atttaccaat gaacttgaaa aaacgattgc tcaatatgtc aaggaagata gtgaacgcta
     8701 cgtgctgaaa tctaagattg agaaacttat tcatcaagaa ttgctccaag acgtccttcc
     8761 ccttcaaagt acagtttgtt aaaatataat tttatggtag cctctcagct tttctgaaga
     8821 cagaaaaaga agctggggct tttttggtgc ttttaaacaa tgtgctagat atttaatcgc
     8881 aatgagttcc tctatgacat gataattaaa gttgagaaag ctagtacttt taatgggagt
     8941 actttttatt tgtgcttatt ttaattaaaa ttatgagtct aatttggaac agacagccca
     9001 gttttgaaca cataaatgtg tgacacttgg tctttttttc aaaagtattt gaacttatac
     9061 tagtagatgt aagaaacaat taatcaggag gaaataaaat gcctaacgtt aaagatatta
     9121 ctagagaatc atggatttta tcaacattcc cagaatgggg aacatggcta aacgaagaaa
     9181 ttgaagaaga agttgttgct gatggcaact ttgcaatgtg gtggttagga aactgtggtg
     9241 tttggattaa aacaccaggt ggtgctaatg ttgttatgga cctatggtca aaccgcggta
     9301 aatcaacaaa aaaagtaaaa gatatggttc gtggacacca aatggctaat atggcaggtg
     9361 ttcgtaaact ccaaccaaac ttacgcgcac aaccaatggt tattgatcca tttgctatta
     9421 atgaattgga ttactatctt gtttcacatt tccacagtga ccacattgat attaacactg
     9481 cggcagccat tattaataat cctaacttag accatgttaa atttgttggg ccatatgaat
     9541 gtggtgaaat ttggaaaaaa tggggagttc cagaagaacg aatcatagtg attaaaccag
     9601 gtgaatcatt tgagtttaaa gacatcaaag ttactgctgt tgaatcattt gaccgtactt
     9661 gcttggtgac acttccagta gatggtgcag aagaacatga tggagaactt gctggtttag
     9721 ccgtgactga tgaagaaatg gctcgtaaag cagttaacta tatttttgaa acaccaggtg
     9781 gaacaatcta ccatggagct gactcacatt tttcaaatta ttttgctaaa catggtaaag
     9841 actacaagat tgacgtagct attaacaact atggtgataa cccagttggt attcaggata
     9901 aaatgacttc aatcgatctt cttcgtatgg cagaaaatct ccgtgccaaa gtcattattc
     9961 ctgtacacta cgatatctgg tctaacttca tggcttctac agacgaaatc ttacaattgt
    10021 ggaaaatgcg taaagaacgc cttcaatatg acttccatcc atttatttgg gaagttggag
    10081 gtaaatacac ttatccacaa gataaagata gaattgaata ccatcaccca cgtggttttg
    10141 atgattgctt cgaacaagaa tcaaatattc aatttaaagc acttctataa tggctaatga
    10201 gagactgtat gtcgcatgat atgcagtttt tcaattttaa aatatgttac taaaatcaca
    10261 attaagatta ggagaaataa tgtcagaatt tgatacaaaa tcatatttag aaaaacttga
    10321 tgcatggtgg agagcagcta attatatttc tgcagcacaa atgtatctaa aggataatcc
    10381 tctcttgaga cgagagctag ttgaaaacga cttaaaggtt catccaattg gtcactgggg
    10441 cactgtacct ggacaaaact ttatctatgc tcacttaaat cgcgctatta ataaatacga
    10501 tttagacatg ttctatattg aaggtccagg tcatggtggt caagttatgg tatctaattc
    10561 atatttagat ggttcatata ctgaattaaa cccaaatatt gagcaaacag aggatggctt
    10621 taagcagtta tgtaaaatct tctctttccc aggtggaatt gcatcccatg cagcaccaga
    10681 aacaccaggg tcaattcatg aaggtggtga acttggttac gcactttctc atgcgacagg
    10741 tgccatccta gacaatccag atgttattgc tgcgactgtt attggtgatg gtgaaggtga
    10801 aacaggacca cttatggctg gttggctatc taataccttt atcaatccag taaatgatgg
    10861 tgctgttcta ccaatcttct atttaaatgg tggtaaaatc cataacccaa ctatctttga
    10921 acgtaagaca gatgaagaat tatctcagtt ttttgaagga ttaggttgga aacctatttt
    10981 tgcagatgtt gttgaactct ctgaggatca tgcggctgct catgctttgt ttgcagaaaa
    11041 attagatcaa gctattcaag agattaaaac cattcaatca gaagcacgac aaaaaccagc
    11101 agaagaagct atccaagcaa aattccctgt cttggttgca cgtattccta aggggtggac
    11161 tggtccaaaa gcttgggaag gaacaccaat tgaaggtggc ttccgcgctc accaagtacc
    11221 aattccagta gatgcccacc atatggaaca tgtcgattct cttttgtcat ggcttcaatc
    11281 ataccgtcca gaagaattat ttgatgaaag tggtaaaatc gttgatgaga ttgctgctat
    11341 ttcaccaaaa ggtgatcgtc gcatgtccat gaatccaata accaatgctg gtattgttaa
    11401 agcaatggat acagcagatt ggaagaaatt tgctcttgat attaatgttc caggtcaaat
    11461 tatggcacaa gatatgattg aatttggaaa atatgcagca gatttggtag atgctaatcc
    11521 agataatttc cgtatttttg gtccagatga aacgaaatca aatcgtcttc aagaagtgtt
    11581 tacacgtact agccgtcaat ggcttggtcg tcgtaaacca gattatgacg aagctttaag
    11641 tccagctgga cgtgttattg attcccaatt gtctgaacat caagcagaag gtttcttaga
    11701 aggttatgtt ttaactggtc gtcacggctt ctttgcttca tacgaatcat tccttcgcgt
    11761 tgtagattca atggtaacac aacatttcaa atggttacgt aaatcaaaaa cacacacaac
    11821 atggcgtaaa aattatccag cgcttaactt aattgcagct tcaactgttt tccaacaaga
    11881 tcataatggt tacactcacc aagatccagg tatcttaact cacttagctg aaaaaacacc
    11941 tgaatacatt cgtgaatatt taccagcaga tactaactca cttctagctg ttatggataa
    12001 agcgtttaaa gctgaagata aaattaattt aattgtgaca tctaagcacc ctcgtccaca
    12061 attttactct attgctgaag cagaagagtt agtggcagaa ggatataagg tgattgactg
    12121 ggcttcaaat gtgtcgctta atcaagagcc agatgttgtc tttgctgcgg cgggaacaga
    12181 acctaactta gaagctttgg cagctattag tattcttcat aaggctttcc cagaacttaa
    12241 gattagattt gttaatgtat tagacatcct aaaacttcgt catccttcac aagatgctcg
    12301 cggtttatca gatgaagagt ttgacaaagt ctttaccaca gataaacctg ttatttttgc
    12361 tttccatagt tatgaagata tgattcgaga tattttcttt agtcgtcaca atcataattt
    12421 gcatacgcat ggttaccgtg aaaatggtga tatcacaaca ccatttgata tgcgcgtgat
    12481 gtcagaattg gaccgattcc atctggcaca ggatgcagct cttgcttctt taggaaatga
    12541 agcacaagcc ttcagtgatg aaatgaatca aatggttgct tatcacaaag actatattcg
    12601 tgaacacgga gacgatattc cagaagtgca aaattggaaa tgggaaaata tcaagtagtc
    12661 acttttttca ggcctatact tacaaacaaa aacactttta gattttttct aagggtgttt
    12721 tgttatttta tagaagaatg accgcttttt caaaagcgtt aagtggtcac ttttaaagtt
    12781 gtaatattaa tactaataag tctttaaata attcaataat gccataacaa taatggtgtg
    12841 ggctaattaa aatatctcta agcaaaggtg ttgacataaa gagaccatac atgatattat
    12901 agttatgttc tgtttcggca gtttattttt ttgagtaaaa aagtgactac ccttttacga
    12961 tactttaacg tcactttata gataagacac ataaccttaa aaataagata cgaaattttt
    13021 agtaggagat aagaatgaca aatattttag aagtgaagaa tttaaccaaa atttttggga
    13081 aaaagcaaaa agctgctctt gaaatggtta agcaaggaaa aagtaagaca gaaattcttg
    13141 aaaaaacagg agcgactgta ggtgtctatg atgcgagttt tgaaataaaa gaaggcgaaa
    13201 ttttcgttat catgggactt tctggttcag gaaaatcaac tttagttcgg atgttaaacc
    13261 gtttgattga cccatcatcg ggaaatatct atttagatgg taaagatatt gcaaagatga
    13321 atgtagaaga tttacgtaac attcgtcgtc acgatatcaa catggttttc caaaattttg
    13381 gtcttttccc acatcgtaca attttagaaa atacagagtt tgggttagag atgcgtggtg
    13441 tttctaagga agaacgtaca atgttagctg aaaaagcttt ggacaatgca ggtctattac
    13501 cgtttaaaga tcaatacccc agtcaattat caggaggaat gcaacagcgt gttggtttag
    13561 cacgtgccct tgctaacagc cctaaaatcc ttctgatgga tgaggccttc tcagctttag
    13621 acccattaat tcgtcgtgaa atgcaagatg agcttcttga tcttcaagac acgaataaac
    13681 aaactattat ttttatcagc catgatttga atgaagcgtt acgtattggt gatcgtattg
    13741 ctctgatgaa agatggggaa atcatgcaga ttggtacagg agaagagatt ttgactaatc
    13801 cggctaatga ttttgttcgt gagtttgttg aagacgttga tcgctcaaaa gtgttaactg
    13861 ctcaaaatat tatgataaaa cctttaacaa ctgttttaga aattgatggc ccacaagttg
    13921 ctttaacaag aatgcatcgc gaggaagtct caatgttaat ggcaacgaat cgtcgtcgcc
    13981 aacttctggg atccctaact gcagatgctg caattgaagc tcgtaagaaa ggtttgccgc
    14041 tttctgaagt gattgataaa gatgttgtaa cggtctcaaa agatacagtt attacagata
    14101 tcatgccttt aatttatgat tcatctgctc cgatagccgt tactgatgat aatgatagac
    14161 tcctaggggt tatcattaga ggacgtgtga tcgaagcact tgctaacgtt caagatgaaa
    14221 cagtagtaga atcgccaaaa gaaacggtag aagcttaata gagaggacaa atcattttgg
    14281 aaaatttatt acaacataag ttaccggtcg caccatttgt agagtcaacg accaattgga
    14341 ttacgaaaac tttttctggt ttatttgact ttatccagac aatcggtaat gcattgatgg
    14401 attggatgac aaaaacatta ctattcatta atcctttact ttttattgta ctaattacag
    14461 ttgctgtatt tttcttagct aagaaaaaat ggcaattacc gacatttact ttcattggtt
    14521 tgctatttat ctataaccaa gggctgtggg agcagttgat taatactttt aacttggttt
    14581 tagtagcaag tttaatttct attattattg gtgtaccact aggtatttgg atggcaaaaa
    14641 gtgataaagt taaacaagtt gttaatccaa ttcttgactt tatgcaaact atgccagcat
    14701 tcgtttacct tattcctgca gtagccttct ttggtatcgg tatggtacca ggtgtctttg
    14761 cctctgttgt atttgctttg cctccaacag tgcgctttac taacttggct attcgtgaaa
    14821 tcccacttga attaatagaa gcatctgatt catttggttc aacagctaag caaaaattat
    14881 tcaaagttga actaccactt gctaagaaca ctattatggc aggaatcaat caaactatga
    14941 tgttggctct ttctatggtg gttacaggat ctatgatcgg tgcacctgga cttggtcgtg
    15001 aggtgttatc ggcgcttcaa catgctgata ttggtacagg atttgttagt ggtctatcgc
    15061 tagttatttt agcaatcgtg ttagaccgtg ttagtcaatt ctttaattcc aaaccgggtg
    15121 agaagcaagc taaaacatca aaagttaaaa aatgggtagg tttaggggcc ttggttctct
    15181 tcattctagc agctcttggt cgcattgttg tcaatatgac ttcaggaaat gaagccaaag
    15241 gtcaaaaagt gaaaatagct tatgtccaat gggattcaga agttgcttca accaacgtta
    15301 ttgctgaagt tttaaaatct aaaggatatg atgttgaatt gacaccactt gataatgcgg
    15361 tcatgtggca aacagtcgcc aatgggaatg ctgattttac aacaagtgct tggttgccaa
    15421 agacccacgg gcaatatttt aataaataca aaaatagttt agatgactta ggtcctcacg
    15481 ttgagaacgt caaaataggt ttagttgttc ctaaatacat gaacgtaaat agtattgaag
    15541 aattatcaaa tcaagcagat aaacaaatta caggtatcga accaggtgca ggtattatga
    15601 agtctgctaa acaatccctt aaagattatc caaacctttc aagttggaag ttgttatcag
    15661 catcaacagg agctatgaca acaaccttag ggaaggctat caaaaacaaa gatcaagtag
    15721 tcattacagg ttggtcacct cactggatgt ttgctaagta tgatttaaaa taccttaaag
    15781 atcctaagaa atcatttggt ggtgaagaac atatcaatac aatagcacgc aagaatttga
    15841 aaaaagatat gccaaaagta tataaaatta tcgataagtt caagtggact aaagaagata
    15901 tggaatctat catgttagat atggataaag gtatggagcc tgctaaagca gctcaaaaat
    15961 ggattaaaaa ccacaaaaaa gaagtttcag agtggactaa
//
LOCUS       DBJYSH010000044        15642 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.044, whole genome shotgun sequence.
ACCESSION   DBJYSH010000044 DBJYSH010000000
VERSION     DBJYSH010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 15642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15642
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.044"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     repeat_region   4..563
                     /inference="COORDINATES: alignment:CRISPRCasFinder:4.3.2"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=4..35
                     /rpt_unit_seq="atttcaatccacgcacccgcaaagggtgcgac"
     gene            complement(710..1003)
                     /gene="cas2"
                     /locus_tag="ACUBV3_001062"
     CDS             complement(710..1003)
                     /gene="cas2"
                     /locus_tag="ACUBV3_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002280569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HHU3847569.1"
                     /translation="MMVLVTYDVNTKTVAGRRRLRHVAKLCVDYGQRVQNSVFECSVT
                     PAEFVEIKNELLTIIDQKSDSIRFYLLGKNWQNRVETIGKNDSYDPDIGVLLL"
     gene            complement(1014..2039)
                     /gene="cas1c"
                     /locus_tag="ACUBV3_001063"
     CDS             complement(1014..2039)
                     /gene="cas1c"
                     /locus_tag="ACUBV3_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019314881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated endonuclease Cas1c"
                     /protein_id="HHU3847570.1"
                     /translation="MKKLLNTLYLTQDNFYLTRERDNIVIKQEGKIVHRFPYRIIDGI
                     ICFSYLGASPSLIELCTEKQINLSFHTPQGRFCGRFVGSTNGNVLLRREHYRLADHAL
                     SIEYAKRFILAKVSNSRKYLIRFKRDHRDRIDIQLFEDINTELMWALELIQSAPDKET
                     LLGIEGQAANQYFRAFNELVLTDKDTFRFNGRTRRPPQDCINALMSLGYSLLTYECQS
                     ALEAVGLDSYVGFYHTDRPGRASLALDLVEEFRSYIVDRFVFSLVNKGQLNKRHFDIK
                     ENSSVILTEKGRTIFIESWQKRKHTVVEHPFTKEKVKLMLLPYVQAQLLSKAIRGDLE
                     SYPPFLI"
     gene            complement(2036..2707)
                     /gene="cas4"
                     /locus_tag="ACUBV3_001064"
     CDS             complement(2036..2707)
                     /gene="cas4"
                     /locus_tag="ACUBV3_001064"
                     /EC_number="3.1.12.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HHU3847571.1"
                     /translation="MVYAEDDYLMLSGIQHFQFCKRQWALIHIEQQWLDNEATAHGQI
                     LHTKADNPYIKEKRKDFLVSRAMHVSSKILGLYGILDVVEFYKDENGFSLKGKRGKWL
                     PCIVEYKRGKPKKDRRDIVQLVAQTICLEETFGCYIEKGCLYYHSVNQKKTIEITEEL
                     RQEVLDLAEQMHFYYDNKIIGKAEYFKNCPLCSLVDICKPRLSKKTRNVDNYIRQSLV
                     NEDSL"
     gene            complement(2709..3557)
                     /gene="cas7c"
                     /locus_tag="ACUBV3_001065"
     CDS             complement(2709..3557)
                     /gene="cas7c"
                     /locus_tag="ACUBV3_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas7/Csd2"
                     /protein_id="HHU3847572.1"
                     /translation="MLEHKIDFIVTFEVKEANANGDPLNGNMPRTDAKGYGVMSDVSI
                     KRKIRNRLQDMGKPIFVQANERIEDDFRSLEKRFSQHFTAKTPDKEIEEKANALWFDV
                     RAFGQVFTYLKKSIGVRGPISISMAKSLEPIVISSLQITRSTNGMEAKNNSGRSSDTM
                     GTKHFVDYGIYVLKGSINAYFAEKTGFSQEDAEAIKEVLVSLFENDASSARPEGSMRV
                     REVFWFTHSSKLGNVSSARVFDLLEYNQSIEEKSTYDAYQIHLNQEKLAKYEAKGLTV
                     KILEGL"
     gene            complement(3562..5457)
                     /gene="cas8c"
                     /locus_tag="ACUBV3_001066"
     CDS             complement(3562..5457)
                     /gene="cas8c"
                     /locus_tag="ACUBV3_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas8c/Csd1"
                     /protein_id="HHU3847573.1"
                     /translation="MDFFTSLLKTYEKAELADLVDHQKRNNEPVLLPIYHTSLKSNGK
                     NIISVKLDKDGQFHKAEFMTDKQMIIFPVTADSVARSGSNPAPHPLVDKFAYYSAEMG
                     QSQYDSFHKQLNNWIDYCEEGDVKRFLTIVQQFILKPEFLTLILDSLIGPDYQNNQLK
                     VTFCDATGKEKLIDLSACFLEFSIDQFQGFKNESVSTFKTLHQSYISFVEANRENLGI
                     CNISGREEQLTDKHRGLMGNAKIISVSNKREAYKGRFREREDVFSVGYETSEKIHLMI
                     KYLLENKNTSTWLGSSQYLINWFSDDLTNDSRLDIVSPIFDDGLEEDDDEDMPSVIAL
                     ATEENKRIGKSFIKGQKLFANDATYYIAILNKTSNGRIALKYFRQIQASQLLTNLDKW
                     QEKYSWESRSKSGKSRLRTPAFHDILNVSYGVDRDRFLELDNDNFKSDQVQKLVTSLI
                     DGKPMPQSIVKKLGNNVKERHRYRKHWYQVEQVCLAILHKQNGEEFSPMLNHTNQNRS
                     YLFGRLLAIFELIETLRYSLEGNGSDRITNAERYWTAYTGQPTKLMMLLENKIKPYEE
                     TLKLNRRGSWKKLEKEKEEILELLNPLLETETMEKPLDYRFIFGYYAEKNYYYPKQNT
                     EVTESEE"
     gene            complement(5457..6185)
                     /gene="cas5c"
                     /locus_tag="ACUBV3_001067"
     CDS             complement(5457..6185)
                     /gene="cas5c"
                     /locus_tag="ACUBV3_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas5c"
                     /protein_id="HHU3847574.1"
                     /translation="MYRSRDFYVRVSGQRALFTNPATKGGSERASYSVPTRQALNGIV
                     DAIYYKPTFTNIVTEVKVINQIQTELQGVRALLHDYSADLSYVSYLSDVVYLIKFHFI
                     WNEDRKDLNSDRLPAKHEAIMERSIRKGGRRDVFLGTRESVGLVDDISQEEYEAAVSY
                     YNGVNIDLGIMFHSFAYPKDKKTPLKSYFTKTVMENGVITFKTQSDCDIVNTLSSYAF
                     KVPGEIKSVNDEYMEYDAMEKGEN"
     gene            complement(6318..8741)
                     /locus_tag="ACUBV3_001068"
     CDS             complement(6318..8741)
                     /locus_tag="ACUBV3_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000988347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HHU3847575.1"
                     /translation="MMTKETLAHYQKKIEQESEKKQSLDEHLWHVACFSRQEASIIGQ
                     GDVLFLISLYHDLGKADRAFQDKLLNNPNRHVDHSYAGAKYLCSIIGPHLKSRGVDKD
                     ERMTFNEMVGYVISAHHGMYDFCYCSDDAEYYSFNKFKNRINRDLDDYHYHEDIKGYA
                     IKLEEKLCDYGYKDLRELIDKAFDNYQQAMSSLNWQDNSEWDYYQSCMVRLYLSLLKN
                     ADILDTVNAYGLEISPMDKTERSSLKHSYLAAIEQKYASFGRPNNQLNTIRTEIAERV
                     KERGKRDSKGIYRLDLPTGAGKTNLSMRYAFHQLVHQDKSRFFYITPFLSVLEQNASE
                     IRKVTGDLGVLEHHSNMVKQANEDDDKDSLLSAYLIDSWDSQVVLTSMVQFFQTLFKT
                     KSANLRRFSSLINSVVILDEVQSLPIEVTTLFNLTMNFLNKVMDTTIVLCTATQPAYD
                     SSEIDHRICYGGNLGELAEIVELTIEEKQIFSRTELRKFDDSDQKVYLTDVINLILGE
                     ENSVLAIFNTKKTVHNCYTMLKDMTDRPIYQLSTNMCAQHRLDLVAKIKTELQNNTPI
                     ICISTQLIEAGVDVDFHRVIRSYSGIDSIVQAAGRCNREGKRDKGQVTLVNLTNEEEN
                     ISRLTEIKAKKEATESILYKIGSPINISTLNRDFFEYYYANNQGLMDYPLEDNISIYD
                     YLSLNTYQTANKKFKGKLKQAFKTAGAKMNLINNDMIGILVPYGDAEKKLAYLEELGT
                     SHFLSAEDYQTIKSLLKELQPFTVNVRENDPLLEATKSYLNGQILVLTSEYYDTERGV
                     KYDSASFYF"
     gene            9753..10532
                     /locus_tag="ACUBV3_001069"
     CDS             9753..10532
                     /locus_tag="ACUBV3_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3847576.1"
                     /translation="MLNSDFIISKSLANYIHHRRLEVGVSSTDLAEISNMSKSDWESF
                     EKNGGAIPLKSKDIILDLLFLERFPKEKECDFIDKLFEEAKENKLWPEKIYQTMGLTP
                     ALSFIAGCEILSDDINNDLEELSKLPKESHLGQLDTSLLLSLLPQQFITKYDYEFVYK
                     LSKVLAQYTSRNKVGSSYTAHNVIEEICLYLIAKESVLYFESLDENSHLQLKELLDYN
                     DEWPFDIFDDMDSYTFLYTDIYIEEDSPYHFKNWFVPQFYL"
     gene            complement(10750..13401)
                     /locus_tag="ACUBV3_001070"
     CDS             complement(10750..13401)
                     /locus_tag="ACUBV3_001070"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009732359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HHU3847577.1"
                     /translation="MSKELSPKYNPAEVEAGRYQTWLDQDVFKPSGDAEAKPYSIVIP
                     PPNVTGKLHLGHAWDTTLQDIIIRQKRMQGFDTLWLPGMDHAGIATQAKVEERLREQG
                     ISRYDLGREKFLDKVWEWKDEYAATIKSQWGKMGLSVDYSRERFTLDEGLSKAVRKVF
                     VDLYNRGWIYRGEFIINWDPAARTALSDIEVIHKDVEGAFYHMNYMLEDGSRALEVAT
                     TRPETMFGDVAVAVNPEDPRYKDLIGQNVILPIINKPIPIVADEHADPEFGTGVVKIT
                     PAHDPNDFAVGQRHNLPQVNVMNDDGTMNELADEFNGMDRFEARKAVVAKLESLGNLV
                     KIEKMTHSVGHSERTGVVVEPRLSTQWFVKMDQLAKNAIANQDTEDKVEFYPPRFNDT
                     FMSWMENVHDWVISRQLWWGHQIPAWYNVNGEMYVGEDAPEGDGWTQDEDVLDTWFSS
                     ALWPFSTMGWPDTEAADFKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGRQPFSNVL
                     IHGLIRDEEGRKMSKSLGNGIDPMDVIEKYGADALRWFLSNGSAPGQDVRFSYEKMDA
                     SWNFINKIWNISRYILMNNEGLTLDQARENVEKVVNSQVGNVTDRWILHNLNETVGKI
                     TENFDKFEFGVAGHILYNFIWEEFANWYVELTKEVLYSDNEDEKVVTRSVLLYTLDQI
                     LRLLHPIMPFVTEEIFGQYAEGSIVLASYPQVNATFENQTAHKGVESLKDLIRSVRNS
                     RAEVNVAPSKPITILVKTSDSELESFFKDNSNYIKRFTNPETLEISSAIATPELAMSS
                     VITGAEIFLPLADLLNVEEELARLEKELAKWQKELDMVGKKLSNERFVANAKPEVVQK
                     EKDKQADYQAKYDATQERIVEMQKLVK"
     gene            complement(13423..14115)
                     /locus_tag="ACUBV3_001071"
     CDS             complement(13423..14115)
                     /locus_tag="ACUBV3_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3847578.1"
                     /translation="MNDFFNWLITLYNDDKVAFVSLCLTLVFGFFSWLAQLKRNKQDD
                     IRQAEQDEFNRLAQDELKEFQSFQKKFAEYQQKVSELQFNFESQENFKPYFEFSKTKS
                     STYVEEGKLIARICLTNVGRGTATKITTNPIDYDQDGNPMYFRTIPTKQTHAIYDYFS
                     ETFAIPNDSIFLSTTALEQTENQNHCIEFKIKFCDSSGRTYEQKFEFGYDNFLVRGIN
                     LKSVTYEPTLIK"
     gene            complement(14112..14690)
                     /locus_tag="ACUBV3_001072"
     CDS             complement(14112..14690)
                     /locus_tag="ACUBV3_001072"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HHU3847579.1"
                     /translation="MQILEDFDGKALPKLETDRLILRQRTVGDVPAMFDYVCLEEVAY
                     PAGLSPIASLEDEYDYFENRYYQNLEKAKLPSGYGITVKGSDRIIGSCDFNHRHEDDV
                     FEIGYMLHPDYWGHGYMTEAVAALIEVGFTLLNLHKIEIRCYDYNKQSQRVAEKLGFT
                     LEATIRDRKDNQGNRCVNLIYGLLRSEWEGRK"
     gene            complement(14958..15269)
                     /locus_tag="ACUBV3_001073"
     CDS             complement(14958..15269)
                     /locus_tag="ACUBV3_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001119099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3847580.1"
                     /translation="MAKRIKPNKKRELLKAKGINWKDVRLSQTLQEVASPKKKAPEAP
                     KAVDLLEGLTANKQDVLKEAGLVSLEAFAKVSEADVLALKGIGPAAIKQLVDNGVVFA
                     K"
     gene            complement(15378..15632)
                     /locus_tag="ACUBV3_001074"
     CDS             complement(15378..15632)
                     /locus_tag="ACUBV3_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1912 family protein"
                     /protein_id="HHU3847581.1"
                     /translation="MSYEQEFLKDFEEWLQSQISINQMAMDSAKKVLEEDRDERAADA
                     YIRYESKLDAYRFLQGKFNNYHNQKSFHDLPDGLFGQRHY"
BASE COUNT         4786 a         3157 c         2532 g         5167 t
ORIGIN      
        1 aatatttcaa tccacgcacc cgcaaagggt gcgaccaatt ttcagacggt acagaagtta
       61 atacagtaaa tttcaatcca cgcacccgca aagggtgcga cacttctaaa aagttttaca
      121 tttgcagact ttattatttc aatccacgca cccgcaaagg gtgcgacacg acttaaagat
      181 tgcgttgaga tagcttatga ttatttcaat ccacgcaccc gcaaagggtg cgaccaaaag
      241 cgaaaaagga gcctaacaga tgaaattatt tcaatccacg cacccgcaaa gggtgcgaca
      301 aaatggtata atagaaggag ctacaaagaa agatttcaat ccacgcaccc gcaaagggtg
      361 cgacaattat tacgcaagaa cacagccaaa atatattatt tcaatccacg cacccgcaaa
      421 gggtgcgact ttgaatgggt caccgcccaa ttctttaata cctatttcaa tccacgcacc
      481 cgcaaagggt gcgacgtgga acattttgct tttatgcgaa ttattttaga tatttcaatc
      541 cacgcacccg caaagggtgc gacagtgcgc gattgcaccg tttataacta aaaccaagaa
      601 tttattaaac aagttggttc aataaaaact agattttgac tactattttc ttttttttag
      661 cgcgaatact ccgctttttc atgtgtgacc cgaattcaca tataaatgtt tatagaagta
      721 atacccctat atcaggatca taactatcat ttttgccaat agtttccaca cgattttgcc
      781 aatttttacc aagtaaataa aatcggatac tatctgattt ctggtcgatg attgtcagca
      841 actcgttttt tatctccaca aactcagctg gagtcactga acactcaaaa acagaatttt
      901 gtacacgttg accataatca acacaaagtt ttgcgacgtg acgaaggcgt ctcctacctg
      961 ctacagtctt cgtattgaca tcataggtta ctaaaaccat cataatttta ctcctagatt
     1021 aaaaatggtg gataggattc caaatcacca cgtattgctt tagataataa ctgtgcttgc
     1081 acataaggca gtagcatcaa cttaactttc tccttggtga aaggatgttc aactactgta
     1141 tgcttgcgct tttgccaaga ctcaataaag attgtacgtc ctttttctgt caaaataaca
     1201 ctactatttt ccttaatatc aaaatgtctt ttatttaatt gtcccttatt aaccagagaa
     1261 aaaacaaatc gatcaacaat ataagaacga aactcttcta ccaaatccaa agctaaactt
     1321 gctcgaccag gtctgtcggt atgataaaat ccaacgtaac tatctaatcc aactgcttct
     1381 aaagcagatt gacattcgta ggtcaacaga ctataaccca atgacatcag agcattgata
     1441 cagtcttgcg gtggccgtcg tgtccgaccg ttaaaccgaa atgtatcctt atccgttaag
     1501 acaagttcat taaaagcacg aaagtattga tttgctgctt gtccttctat tcctagcaga
     1561 gtttccttat caggtgcaga ttgaattagt tctaacgccc acatcaattc agtattaatg
     1621 tcttcaaaca attgaatatc aattctatct cgatgatcac gtttaaaacg aattaaatat
     1681 tttctagaat tagatacttt tgccaatata aagcgtttgg catattctat tgaaagagca
     1741 tggtccgcta atcgataatg ttcacgtcta agcagaacat tcccattcgt tgaacctaca
     1801 aagcgaccac aaaaccgacc ttgtggagta tggaaagaaa ggttaatttg tttctctgta
     1861 caaagttcaa ttaaggatgg tgaagcacct aaatatgaaa aacagataat accatcaatt
     1921 atacgatatg ggaagcgatg aacaattttc ccctcctgct taataacgat attatctcgc
     1981 tcccgtgtga gataaaaatt gtcctgtgtg agatataaag tatttaacaa ttttttcata
     2041 aactatcctc atttaccaaa gactgtctaa tgtaattgtc tacgttacgt gttttcttgc
     2101 tcagccgtgg tttacaaata tctaccaaag aacaaagagg acaattttta aaatactcag
     2161 ctttacctat gattttatta tcataataaa aatgcatttg ttcagccaaa tccaaaactt
     2221 cttggcgtag ttcctcagta atttcaatag ttttcttttg attcacgctg tgataataaa
     2281 gacagccttt ctcgatatag cacccaaaag tctcctctaa acaaattgtc tgtgctacta
     2341 gttgaacaat atctcgtcta tcttttttcg gtttacctcg cttatattca acaatacaag
     2401 gaagccactt ccctcgtttt ccctttaaag aaaagccatt ttcatcttta taaaattcaa
     2461 caacatcaag aataccatat agaccgagaa tttttgatga cacgtgcata gcacgtgaga
     2521 ccaaaaaatc ttttcgcttt tctttaatgt aagggttatc tgctttagta tgtagaatct
     2581 gcccatgcgc tgtcgcttca ttatcaagcc attgttgctc aatatgaatc aatgcccatt
     2641 gacgtttaca aaattggaaa tgttgaatac ctgataacat caaataatca tcttcggcat
     2701 agaccatatt atagcccttc taggatttta accgttaaac ctttcgcttc atatttagct
     2761 agtttttctt gatttagatg aatttgataa gcgtcataag tgcttttttc ttctattgat
     2821 tgattatact ctaacaagtc aaagacacgc gcacttgaaa catttcccaa tttgcttgaa
     2881 tgagtaaacc aaaagacttc gcgaactcgc atagaacctt ctggacgtgc agacgatgca
     2941 tcattttcaa ataagctaac caaaacttct ttaatagcct cagcatcttc ctgagaaaaa
     3001 ccagtctttt cagcaaaata agcattgata gaacctttaa gcacataaat accataatct
     3061 acaaaatgtt ttgtccccat agtatcagaa gagcggccac tattattctt agcttccata
     3121 ccattggtac tacgtgtaat ttgaaggcta gaaatgacaa ttggctccaa ggactttgcc
     3181 atactgattg aaatcggtcc gcgcacccca attgattttt tcagataagt aaaaacttgt
     3241 ccaaaagcac gaacatcaaa ccataatgca tttgcttttt cttcaatttc tttgtcaggt
     3301 gtcttagctg taaaatgttg cgaaaagcgt ttttccagtg aacgaaagtc gtcttcaata
     3361 cgctcattag cttgcacaaa aataggtttc cccatatctt gcaaacgatt acgaatcttc
     3421 cgtttaatgg agacatcact cattacaccg tatcctttag catctgtacg aggcatgttt
     3481 ccgtttaatg gatcaccatt tgcatttgct tctttcactt caaaggtcac tataaaatca
     3541 attttgtgtt ccaacatctt tttactcctc actttctgtt acttcagtat tttgttttgg
     3601 atagtaatag tttttctcag cataataccc aaaaataaag cggtaatcta acggtttctc
     3661 cattgtttct gtttctaata gaggatttaa aagttctaaa atttcttctt tttctttttc
     3721 taatttcttc caactgccac gacgatttaa tttcaatgtt tcttcgtaag gcttaatttt
     3781 attctccaat aacatcatca attttgttgg ctgtccagta taggctgtcc aataacgttc
     3841 agcattagta atacgatcgc tgccgtttcc ctccaagcta taacgcaagg tctcaattaa
     3901 ttcaaaaatt gctaataatc gtccgaaaag ataggaacga ttttgattgg tatgatttag
     3961 catcggtgaa aactcctccc cattttgttt gtgtaaaatt gctaagcaga cctgctcaac
     4021 ttgataccag tgcttacgat aacgatgtcg ttctttaaca ttgttaccta attttttgac
     4081 aatagactgt ggcataggtt taccatcaat caaacttgtc actaattttt gaacttgatc
     4141 acttttgaag ttatcattgt ctaattcaag gaagcgatcc ctatcaactc cgtaggacac
     4201 attaaggatg tcatgaaagg caggggttct taagcgactc ttcccagact tagatcgcga
     4261 ttcccaactg tatttttcct gccacttgtc aagattggtg agtaattggg acgcttgaat
     4321 ctgacgaaaa tattttaaag caattctccc gttgctggtt ttattcaaaa tagcaatgta
     4381 ataagtggca tcgttagcaa ataatttttg tcccttgatg aatgatttac caatcctttt
     4441 attttcttca gttgctaatg ctataacaga gggcatatcc tcatcatcat cttcttcaag
     4501 tccatcatca aagattggtg atacgatatc caaccgacta tcatttgtta aatcatcact
     4561 aaaccagttg attaaatatt gagaagaccc taaccaagta ctggtatttt tattttctaa
     4621 aaggtacttg atcattaaat gaatcttttc ggaagtttca tagccaacac taaaaacatc
     4681 ttcacgttct ctaaaacgtc ctttataagc ttctctttta ttactaacag agatgatttt
     4741 agcattcccc atcaaacctc tatgcttatc ggtaagctgt tcctctcgtc cactaatatt
     4801 acaaatacca agattttcac gattggcttc aacgaaagaa atataggatt ggtgtaaagt
     4861 tttaaatgtc gaaaccgatt catttttaaa gccctggaac tgatcaattg aaaattctaa
     4921 aaagcaagct gataaatcaa ttaatttttc ttttccagtg gcatcacaaa atgtgacttt
     4981 taattgatta ttttgataat caggaccaat taaagaatcg agaatcagtg tcagaaattc
     5041 tggtttcaaa ataaattgct gaacaatggt taaaaacctc ttgacgtcac cctcctcaca
     5101 ataatcaatc cagttattaa gttgcttatg aaaagaatca tactgggact gtcccatttc
     5161 agcactataa taagcaaatt tatcgactag gggatgcggc gcaggattac tacctgacct
     5221 agcaacagaa tcagccgtta caggaaaaat aatcatttgc ttatctgtca taaattctgc
     5281 cttgtgaaac tggccatctt tgtcaagttt gactgaaatg atatttttgc catttgactt
     5341 taaactcgta tgataaattg gcagtaaaac cggctcatta tttctttttt ggtgatcaac
     5401 caagtctgct agctctgctt tttcataagt cttcaagaga gaagtaaaaa aatccatcag
     5461 ttttctcctt tctccatcgc atcatactcc atgtattcat cgttaaccga ttttatttcc
     5521 cctggcactt taaaagcata actagaaagt gtgttaacaa tatcgcaatc agactgtgtt
     5581 ttaaaagtaa tgactccatt ttccatcaca gtctttgtaa agtatgattt caatggtgtc
     5641 tttttgtctt tcggataggc aaaggaatgg aacatgattc ccaagtcgat attgacacca
     5701 ttataatacg acacagcagc ctcatactct tcttggctaa tatcatctac aagacctaca
     5761 ctctctcttg tacccaaaaa cacatcacga cgtccacctt tacgaataga acgctccata
     5821 atggcttcat gtttagctgg aagtctgtct gagttcaaat cttttctatc ttcattccaa
     5881 ataaaatgaa atttgattag ataaacaaca tcactcaaat aggatacata acttaaatct
     5941 gcactataat catgtaacag agccctgaca ccctgtaatt cggtttgaat ctggttaata
     6001 accttaacct ctgtgacaat attagtaaag gtcggcttat aatagatggc atcaactata
     6061 ccattcagcg cctgtctagt tggaaccgaa taggatgcac gttccgatcc cccttttgtg
     6121 gctggatttg taaaaagggc tcgctgacca cttactctca cgtagaagtc tctagatctg
     6181 tacaagatat tttcctcctt ttgttttttt ataatctaat tttattaaat agtctaaatt
     6241 gataataatt attaatgatg attggcaggt taacaagatc ctctaccaac cttttgttaa
     6301 tctgctttcg ttttgagtta gaagtaaaag ctagctgaat cgtatttaac tcctctttcc
     6361 gtgtcataat actccgacgt caaaaccaga atctgaccat ttagataaga ttttgttgcc
     6421 tcaaggagag gatcgttctc acggacatta accgtaaaag gttgtaactc ttttagtaat
     6481 gattttatcg tttgataatc ttctgctgat aaaaaatgtg acgtacctaa ttcttctaaa
     6541 taagccaatt ttttctcagc atcgccataa ggtacgagaa ttcctatcat atcattattg
     6601 atgaggttca ttttggctcc tgctgtttta aaagcttgtt ttaacttacc tttgaacttt
     6661 ttatttgccg tctgataagt attaaggctt aaatagtcgt agattgatat gttgtcttcc
     6721 aaaggataat ccatcagtcc ttgattattg gcataataat actcaaaaaa gtcacggttt
     6781 aaagttgaga tattgattgg agacccaatc ttataaagaa tagattctgt ggcttctttt
     6841 ttagctttta tttctgtcag cctagaaata ttttcctctt cattggtcag attgacaaga
     6901 gtgacctgac ctttatctcg tttgccttct cgattacatc gtccagcagc ctgaacaata
     6961 gaatcaatcc ctgaatagga acgaatgaca cgatgaaagt caacatctac acctgcttct
     7021 attaattgtg tgctaataca aataataggg gtattatttt gtaactccgt tttgatctta
     7081 gcaaccaagt caagcctatg ctgcgcacac atattcgtcg aaagctgata gatcggtcta
     7141 tcagtcatgt ctttgagcat agtatagcag ttatgaaccg tttttttcgt gttaaaaata
     7201 gcaagaactg agttttcctc tcctaaaata agattaataa catcagtcaa gtaaactttc
     7261 tgatcactat catcaaattt tctaagctct gtccttgaaa aaatttgttt ttcttcaatc
     7321 gttaactcaa ctatttcagc taattctccc aagttccctc cataacagat acgatggtca
     7381 atctctgaag aatcataagc aggttgtgtt gctgtacaaa gaacgatggt tgtatccata
     7441 actttattta aaaaattcat cgttaaatta aacaaagtgg tgacttcaat aggcagtgac
     7501 tgaacttcat ctagaatcac aacactatta atcaaacttg aaaaacgtct caaattagcc
     7561 gattttgttt taaaaagcgt ttggaaaaat tgaaccatag aagtcaagac tacttgactg
     7621 tcccagctat caataagata agctgacaat aaactgtcct tgtcatcatc ttcattagcc
     7681 tgtttaacca tattggaatg gtgttctaaa acgccaaggt cacccgtaac ttttctaatt
     7741 tcggaagcat tttgctcaag aaccgaaaga aagggcgtta tgtaaaaaaa tcttgatttg
     7801 tcttgatgaa ctaattggtg aaacgcatag cgcatactaa gattagtctt gccagctcct
     7861 gtcggtaaat ctaagcgata gatccctttg gaatcccgtt tacctctttc tttaacacgc
     7921 tcagcaattt ctgtccgaat agtattcaac tgattgtttg gtcgtccaaa gctagcatat
     7981 ttttgttcaa tggctgctaa ataagaatgt tttagagagg atcgctctgt tttatccata
     8041 ggacttattt caagaccata ggcatttact gtgtccaaaa tatcagcgtt ttttaagagc
     8101 gacaagtaaa gtctcaccat acaagactga taataatccc actcactatt atcttgccag
     8161 tttaaggaag acatagcttg ttggtaatta tcaaaagctt tatcaataag ctctcttaaa
     8221 tctttgtagc cgtaatcaca caatttttct tctaatttta tagcataccc tttaatatct
     8281 tcgtgatagt gataatcatc taagtctctg ttgatacggt ttttaaattt attaaagcta
     8341 taatattcag catcgtcaga acagtagcaa aaatcataca tcccatgatg agccgagatg
     8401 acataaccca ccatttcatt gaatgtcatt ctctcatctt tatcaacccc tcggcttttt
     8461 agatgaggcc caataataga gcataagtat tttgcccctg cataagaatg gtcaacatgt
     8521 cgatttggat tatttaataa tttatcttga aaggctcgat cagctttgcc tagatcgtgg
     8581 tatagactaa ttaaaaaaag cacatctcct tgaccaatta tagatgcttc ctgtcgacta
     8641 aaacaggcca catgccataa gtgctcatct aaagactgct ttttttcaga ttcttgttct
     8701 attttctttt gataatgtgc caacgtttcc ttagtcatca tcaacctctc tccctcatcc
     8761 ataactcata tccctcaatg atatactaac atttttgaaa ggaattgtaa acggatacaa
     8821 tagttaatgc ttcccccaaa aagcctctaa atgactttcc aaaaaaacag gaatcttgac
     8881 aattcaagat ccttgttagg agttctgtaa aaaaaattat ttttcggcta ggcgtagttt
     8941 aagttaggaa acttaaaact agcttaattg gaagatgtta aatttttata acacctaacg
     9001 ctgacaatgc tgataatatc gacctttaca tttggtactt attttttgtc atctcgacta
     9061 gtaaacgtgt gttctaaatc gccaagcatt agttttttca gcacatacaa cttttccttt
     9121 cattcttgcg gagataccat caaaatgcta aattaaattt aacattgaca acaaaacact
     9181 tgttccctaa acagtatcaa taagtcaacc gatcggttta aaatactact gtcttatcgt
     9241 atgtaacccc ctctatctag ttgctggttt caagtagtat gataaaacct acattttatg
     9301 catgcttact accattagaa tggaattgtt atgaccaagg taaagtattc gttatttttt
     9361 ctcatctatc ttgcactact atatctaact agtatctact ttcctgctat ttataaagtc
     9421 ctcatgtatc ttcactttct atgtctattc atttttagca ttatgttcct ttataatctg
     9481 attgtcattt taaaatatat ctttacgcat aaaaagaagt agattagcat atatttttgt
     9541 ttgctcgtta taaaattaag tttttaagga atgttaaaac tactaaacac acccaaattg
     9601 atagataata gaaaatacta ctaatattat gaatcattac aacatctcta gaaaatcatg
     9661 atgtgctaaa tcaaaagatg catgtgtttt tacttgaaaa aaatttttta tatgtgataa
     9721 tcataatgaa taggaatcaa aaggagaacg gaatgctaaa ttctgatttt attattagta
     9781 agtcacttgc taactacatt caccatcgta ggttagaagt tggcgtgtcg tcaactgatt
     9841 tggcagaaat atccaatatg tctaaatcag attgggaatc atttgaaaaa aatggtgggg
     9901 caattccttt gaaatctaag gatatcatac ttgacttatt atttttggag agattcccta
     9961 aagagaaaga atgtgatttt atagataaat tatttgagga agcaaaggaa aataaacttt
    10021 ggcctgaaaa gatttaccag actatgggac ttactcctgc tctttcattt attgctggtt
    10081 gtgaaatact ttctgatgat attaataatg atttggagga attatctaaa ctacctaaag
    10141 aaagtcatct gggacaatta gatacttccc tacttttatc actattgcct caacaattta
    10201 tcacaaaata tgattatgag tttgtttata aattaagcaa agttttggct caatatacat
    10261 caagaaataa agttggttca tcttatacag cgcataatgt aatagaggaa atttgtctct
    10321 atttaattgc aaaagaatcc gttctttatt ttgaatctct ggacgaaaac tcccatctac
    10381 aacttaagga attattagat tataacgatg agtggccttt tgatattttt gatgatatgg
    10441 atagctatac ctttttatat acagacatct atattgaaga agattcacct tatcatttta
    10501 aaaactggtt tgtacctcaa ttttatttat aaaataattg acgaaaaaat ataagaaata
    10561 ggcattaatc atttgggttt ctgcctgttt ttctttatgg ccaaatcatt ttcacttctt
    10621 gtatttcaga cgcaagaagt aggcatcata tatattaatt tttcacaatc tttgaatata
    10681 gcaaaaagcc ctgtcgctta gactgatggt ctagcggcag gactctgcgg aagtaatgga
    10741 tgattttaac tatttaacaa gtttttgcat ctctacaatc cgttcttggg ttgcgtcgta
    10801 tttagcttgg tagtcggctt gtttgtcttt ttctttttgg acaacttctg gtttagcgtt
    10861 ggctacaaag cgctcgttgc tgagtttttt gccaaccata tctagttctt tttgccattt
    10921 agcaagttct ttttcaagtc gtgccaactc ttcttctacg ttgaggaggt cagcaagtgg
    10981 gaggaagatt tcagctcctg tgatgactga tgacatggca agttctggtg tggcaattgc
    11041 tgagctgatt tcaagcgtct ctgggtttgt gaagcgtttg atgtagttgc tattgtcttt
    11101 gaagaagctt tcgagttcgc tgtcacttgt cttaaccaag atagtgattg gtttagatgg
    11161 agcaacattg acctctgctc tgctattacg cacggaacga atcaaatctt tcaagctttc
    11221 aacgcctttg tgagctgttt gattttcaaa tgtcgcgtta acttgtggat aacttgcaag
    11281 gacaattgat ccttctgcat attgtccaaa aatttcttct gtcacaaatg gcatgattgg
    11341 atgaagaaga cgcaagattt gatctaaggt ataaagaaga acagaacgtg taacaacttt
    11401 ctcatcttca ttgtcactat aaagaacctc ttttgttaac tcaacgtacc aattagcaaa
    11461 ctcttcccag ataaagttgt aaaggatgtg accagcaaca ccaaattcaa acttgtcaaa
    11521 gttttcagta attttaccca cagtctcatt taaattgtgg agaatccaac ggtctgttac
    11581 atttccaact tgactattaa caactttttc cacattttct ctggcttgat ccaacgttaa
    11641 accttcgtta ttcataagga tatagcgtga aatgttccaa atcttattaa tgaaattcca
    11701 cgaagcatcc attttttcgt atgaaaaacg cacatcttga ccaggggctg aaccatttga
    11761 gaggaaccaa cgaagggcat ccgcaccata tttctcaatg acatccatcg gatcaatccc
    11821 attgccaagt gatttagaca ttttacgtcc ttcttcatca cggatgagtc catgaataag
    11881 aacatttgag aatggttggc gtcctgtaaa ttcaagggat tggaaaatca tgcgcgatac
    11941 ccagaagaag atgatatcgt atccagttac caaggttgac gttgggaagt agcgtttgaa
    12001 gtcagctgct tcggtgtctg gccatcccat tgttgaaaac ggccataaag cagaactaaa
    12061 ccatgtgtct agaacgtctt catcctgtgt ccatccgtca ccctctgggg catcttctcc
    12121 aacatacatc tcaccattga cgttgtacca tgcaggaatt tggtgtcccc accataattg
    12181 acgagagata acccagtcat ggacattttc catccagctc ataaaagtat cgttaaatcg
    12241 tggtggataa aactctacct tgtcttctgt atcttggttg gcaatggcat tcttagccaa
    12301 ttgatccatc ttgacgaacc attgagtaga taaacgtggt tcaaccacaa cgcctgttcg
    12361 ttctgaatga ccaacagaat gagtcatttt ttcaattttt actaagtttc ctaacgattc
    12421 taatttagcg actactgcct tacgagcttc gaaacgatcc ataccattaa attcatccgc
    12481 aagttcattc atagtaccat catcgttcat aacgttaact tgtggtaagt tgtggcgttg
    12541 acctacagcg aagtcattag ggtcgtgagc cggcgtaatc ttaaccacac ctgttccaaa
    12601 ttctggatca gcgtgttcat cagcgacgat tggaataggt ttattgatga taggaaggat
    12661 aacattttga ccaatcaaat ccttgtaacg tggatcttct ggatttaccg ctacagccac
    12721 atctccaaac atagtctcag gacgagttgt tgctacttct agagcgcgtg agccatcttc
    12781 cagcatataa ttcatatgat agaaagcacc ctcaacatcc ttgtgaataa cttcaatatc
    12841 tgaaagggct gtacgagctg ctggatccca attaatgatg aactccccac gatagatcca
    12901 acctctattg tacaaatcaa caaagacttt tcttaccgct tttgaaagtc cctcatcaag
    12961 ggtaaaacgc tcacggctat aatcaaccga aagacccatt ttcccccact gagatttaat
    13021 agttgctgca tactcgtctt tccattccca aactttatct aggaattttt cacgaccaag
    13081 atcataacgt gaaatgcctt gctcacgaag gcgctcctca actttagctt gagtggctat
    13141 accagcgtgg tccatacctg gaagccagag tgtatcaaag ccctgcatac gcttttgacg
    13201 aataatgata tcttgtagag ttgtatccca agcgtgaccc aagtgcaatt tacccgtaac
    13261 gtttggtggt ggaatcacaa tagaataagg cttagcctca gcatcccctg atggcttgaa
    13321 aacatcttgg tcaagccaag tttgataacg gcctgcctca acctcggcag gattgtattt
    13381 tggtgatagt tcttttgaca tatagtgtcc tttctaattc tcttatttaa tcaaggttgg
    13441 ctcataagtt actgatttca gatttattcc ccttactaaa aaattatcat aaccgaattc
    13501 aaatttttgt tcataagttc tgcctgatga atcacaaaat ttaattttaa actctataca
    13561 atgattctga ttctctgtct gttctagtgc agttgtactc aaaaatatag aatcatttgg
    13621 aatagcaaaa gtctcactaa aataatcata tatagcatgt gtctgtttag taggtattgt
    13681 tctaaaatac attggatttc catcttggtc gtagtcaata ggattggtcg taatctttgt
    13741 agctgtgcct ctccctacat ttgtcagaca tattcttgca atcaattttc cttcttcaac
    13801 gtaagtactt gatttagttt tgctaaattc aaaatagggt ttgaaatttt cttgactttc
    13861 aaaattaaac tgcaattcac tcactttttg ttgatactct gcaaactttt tttgaaaact
    13921 ctgaaattct tttaactcat cttgagctag tctgttaaat tcatcctgtt cagcttgtcg
    13981 aatatcatct tgtttgtttc tttttaattg ggctaaccat gaaaaaaatc caaacactaa
    14041 agttagacaa agtgatacaa aagcaacttt atcatcatta tatagtgtaa ttaaccaatt
    14101 aaaaaaatca ttcattttct cccctcccac tcactcctca gcaaaccata tatcaaattg
    14161 acacagcgat taccttgatt atccttacga tcacggatgg ttgcttcaag ggtgaatcct
    14221 agtttttctg ccacgcgctg gctttgctta ttgtagtcat agcagcggat ttcgattttg
    14281 tggagattga gaagggtaaa accgacttca atcaaggctg cgacagcttc tgtcatatag
    14341 ccatgacccc agtagtcagg atgaagcatg tagccaatct cgaaaacatc gtcttcatgg
    14401 cggtggttaa agtcgcaaga gccgataatc cggtcacttc ctttaacggt aataccataa
    14461 ccagatggta attttgcctt ttccagattt tgatagtagc gattttcaaa ataatcgtac
    14521 tcatcttcta aagaagctat tgggctgagt cctgctggat aggcaacctc ttccaagcaa
    14581 acgtaatcaa acatggctgg gacatccccc accgttcgct gacgcaagat aagccgatcc
    14641 gtttctagtt ttggtaatgc tttgccatcg aaatcttcta gaatctgcat atacttcaat
    14701 ctcctcactc ctaaatacaa aaaatccctg tcaattctga cagggacgaa tttcgtatta
    14761 cgatttccgc ggtaccaccc aaattcggat gataacatcc gcaactcgtt ttgtttcaat
    14821 tattggactt agcaacaagc tttgacattt gtgcggattt ctcagtatga ccgcttcctg
    14881 tgacaaatgg gatttcaaaa cacctctaag ttaatttatt ctagcaaaat aaggctactt
    14941 tcgcaaggta aatcaaatta ttttgcaaaa acaacaccat tatcaactaa ctgtttaata
    15001 gctgctggac caataccttt tagtgctaaa acatctgctt cacttacttt cgcaaacgct
    15061 tcaaggctga caaggccagc ttcttttaaa acatcttgtt tattagcagt taaaccttct
    15121 aaaagatcga ctgcctttgg cgcttcagga gccttttttt tcggtgatgc gacttcttgt
    15181 aaagtttgag atagtcgaac atccttccaa ttgatccctt tagctttcaa aagctctctt
    15241 tttttattcg gtttaatacg ctttgccatt gtataatgct ttctcctcat ttatgaaatc
    15301 tatcatagta tctaaaaact actataacaa tgtatcatag aaatgcctat ttgacaagct
    15361 ttctagcgat aaacctttta ataatgacgt tgtccaaaaa gaccatcagg caaatcatgg
    15421 aaagattttt ggttatgata gttattaaat ttcccttgca aaaagcgata agcgtctaac
    15481 ttactctcat aacgaatata cgcatctgct gctcgctcat ccctatcttc ctctagaact
    15541 ttctttgcac tgtccattgc catttggttg atacttattt gcgattgtaa ccactcttca
    15601 aagtctttta aaaattcttg ttcgtatgac atgatattcc tc
//
LOCUS       DBJYSH010000045        14990 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.045, whole genome shotgun sequence.
ACCESSION   DBJYSH010000045 DBJYSH010000000
VERSION     DBJYSH010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 14990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14990
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.045"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            17..607
                     /locus_tag="ACUBV3_001075"
     CDS             17..607
                     /locus_tag="ACUBV3_001075"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HHU3847582.1"
                     /translation="MPQNPTSNPEKKTYLLSELVDCFKGKAVPSKAEAGDIRIINLSD
                     MSPLGIDYHNLRTFQDEQRSLLKYLLQEGDVLIASKGTVKKVAIFEEQDYPVVASANI
                     TILRPTQHIRGYYLKLFFDSEEGQQALENANKGKAVMNISTKELLNIAIPSIPLFRQD
                     YLIQRYKQGLNDYKRKIARAEQEWERIQNDIRQQLS"
     gene            641..1912
                     /locus_tag="ACUBV3_001076"
     CDS             641..1912
                     /locus_tag="ACUBV3_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HHU3847583.1"
                     /translation="MNEIKCPHCGTAFAINESEYHQLLEQIRGDAFDKEVSERLEKER
                     LILGEQAKNQLQEVVVEKDKEIAKLQYKVKQFLIEKDNLLKDNEYQLAEQLNQKDMML
                     RDLENQIDRLRLEHENSLQEALTKVERERDAIQNQLHIQEKEKDLALASVKSDYEVQL
                     KAANEQVEFYKNFKAQQSTKAVGESLEHYAETEFNKVRHLAFPNAYFEKDNTLSSRGS
                     KGDFIYREKDENDLEFLSIMFEMKNESDDTIKKHKNEDFFKELDKDRREKSCEYAVLV
                     TMLEADNDYYNTGIVDVSHKYPKMYVIRPQFFIQLIGILRNAALNTLKYKQELALMKE
                     QNIDITHFEEDLDIFKNAFAKNYNSASKNFQKAIDEIDKSIKRMEAVKAALTTSENQL
                     RLANNKLDDVSVKKLTRKNPTMKAKFDALKD"
     gene            1925..2356
                     /locus_tag="ACUBV3_001077"
     CDS             1925..2356
                     /locus_tag="ACUBV3_001077"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HHU3847584.1"
                     /translation="MKIRTATLDDSEKLVPLYQELGYAISLSEIQSILKVILTHSDYG
                     FLIAEDNGKLLAFVGYHKLYFFEKSGTYYRILALVVNEKHRRKGIASQLINHVKQLAK
                     TDGSEVLALNSSLKEYRQEAYHFYENLGFKKVSTGFSYYLD"
     gene            2448..3332
                     /gene="truB"
                     /locus_tag="ACUBV3_001078"
     CDS             2448..3332
                     /gene="truB"
                     /locus_tag="ACUBV3_001078"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001876923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HHU3847585.1"
                     /translation="MITGIINLKKEAGMTSHDAVFKLRKILHTKKIGHGGTLDPDVVG
                     VLPIAVGKATRVIEYMTESGKIYEGEITLGYATSTEDSSGEVISRTPLTQSDLSEDVV
                     DHAMKSFTGPITQVPPMYSAVKVNGKKLYEYARSGEEVERPKRQITISEFRRTSPLYF
                     EKGICRFSFYVSCSKGTYVRTLAVDLGIKLGYASHMSFLKRTSSAGLSITQSLTLEEI
                     NEKYKQEDFSFLLPIEYGVLDLPKVNLTEEDKVEISYGRRILLENEADTLAAFYENRV
                     IAILEKRGNEFKPHKVLL"
     gene            3345..4277
                     /locus_tag="ACUBV3_001079"
     CDS             3345..4277
                     /locus_tag="ACUBV3_001079"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HHU3847586.1"
                     /translation="MKIHYINDYKDIQAKEDCVLVLGYFDGLHLGHKALFDKAKKIAT
                     EKNLKIVVLTFNETPRLTFARFQPELLLHLTSPEKRSEKFQEYGVDELYLMNFTSHFS
                     KVSSDLFIKKYIYGLRAKAAVVGFDYKFGHNRTSGDYLARNFKGPVYIIDEISEGGEK
                     ISSTRIRQLITEGNVEKANQLLGYEFSTCGMVVHGDARGRTIGFPTANLAPINRTYLP
                     ADGVYISNVLINGKYYRAMTSIGKNITFGGTELRLEANIFDFDGDIYGETIEIFWLKR
                     IREMVKFNGIDDLVKQLKKDKEIALNWKKDSQTL"
     gene            4320..4733
                     /locus_tag="ACUBV3_001080"
     CDS             4320..4733
                     /locus_tag="ACUBV3_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000631264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HHU3847587.1"
                     /translation="MITLFLSPSCTSCRKARAWLSKHEVAFEEHNIITSPLNKEELLQ
                     ILSFTENGTEDIISTRSKVFQKLAIDVDELSTSSLMELISENPSLLRRPIILDKKRMQ
                     IGFNEDEIRAFLPRDYRKQELKQATIRAEIEGKHD"
     gene            4726..5007
                     /locus_tag="ACUBV3_001081"
     CDS             4726..5007
                     /locus_tag="ACUBV3_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HHU3847588.1"
                     /translation="MISSNYSYPLDPSWNTEDITKVLRFLNQVEHAYENSVKVDDLLD
                     SYKEFKKFVKSKAQEKQIDREFQRTSGYSTYQAVKAAQQQAKGFISLGR"
     gene            4997..5761
                     /locus_tag="ACUBV3_001082"
     CDS             4997..5761
                     /locus_tag="ACUBV3_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HHU3847589.1"
                     /translation="MDAKFDFAKQLVYKAGQFIKSEMQNTFDVEEKSRFDDLVTSLDK
                     KTQKLLIQEIIQHYPDDNILAEEDDVRSPIAQGNVWVLDPIDGTVNFIVQKDNFAVML
                     AYYEEGVGQFGIIYDVMADILYSGGGHFDVYANDKKIVPFQECPLERCLLGVNSAMYA
                     ENDCGIAHLASETLGVRIYGGAGISMAKVMQGKLLAYFSYIQPWDYAAAKIMGETLGF
                     TLLTLDGEEPNYSTRQKVMFLPKSKLNLIQSYLTKK"
     gene            5829..7139
                     /locus_tag="ACUBV3_001083"
     CDS             5829..7139
                     /locus_tag="ACUBV3_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HHU3847590.1"
                     /translation="MKLPNEFIEKYQTILKDEAEAFFDSFEQKPISAYRTNPLKEKQL
                     DFPNAIPSTPWGHYGKISGKSIEHTTGLVYSQEPAAQIVAQIAEPQEGMKVLDLAAAP
                     GGKTTHLLSYLNNTGLLVSNEISNKRSKILVENVERFGARNVIVTNESSQRLAKCFNS
                     FFDLIVFDGPCSGEGMFRKDPQAIQYWHKDYPTECAQLQRDILKEAIKMLAHGGILVY
                     STCTWSPEENEEVVNWLLQEYDYLELVDIPKLNGMVEGINVPQVARMYPHHFQGEGQF
                     VAKLRDTRSKEAQKIKPKAQKINKMQLQLWQQFAQDHLKIDLNGVLDVFGDQLYLLPN
                     GLPDLSKLKIARNGLHLGTFKKNRFEPSFALGMALSEHDLVQSIEIDIEQFEVYVSGN
                     VVKLDKTVPNGWYQILVKGNGLGFAKVTNNTLKNYYPKGLRFQT"
     gene            7318..8184
                     /locus_tag="ACUBV3_001084"
     CDS             7318..8184
                     /locus_tag="ACUBV3_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HHU3847591.1"
                     /translation="MKMKKNIFLLTLLATVLLTLSGCANWIDKGQSITSVGSTALQPL
                     VEAAADEFGKTNLGKTINVQGGGSGTGLSQVQSGAVQIGNSDLFAEEKDGIDAKKLKD
                     HQVAVAGLAVIVNKKVNVKNLTTHQLRDIFAGKIKNWKEVGGQDLDISIINRAASSGS
                     RATFDNTIMGNVAPIQSQEQDSNGMVKSIVSQTPGAISYLAFAYVDKSVGTLKLNGFA
                     PTAKNVTTDNWKLWSYEHMYTKGNETGLTKEFLDYMKSDKVQSSIVQHMGYISINDMK
                     VVKDAEGKVTPK"
     gene            8230..9147
                     /gene="pstC"
                     /locus_tag="ACUBV3_001085"
     CDS             8230..9147
                     /gene="pstC"
                     /locus_tag="ACUBV3_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HHU3847592.1"
                     /translation="MKNQELAKKLTSPSKNSRLEKFGKGITFLSLALIVFIVAMILIF
                     VAQKGLSTFFVDGVKLTDFLFNTKWEPSAKSFGALPMIAGSFIVTILSAIIATPFAIG
                     AAVFMTEISPKYGSKILQPAVELLVGIPSVVYGFIGLQIIVPFVRSIFGGTGFGILSG
                     VCVLFVMILPTVTFMTVDSLRAVPRHYKEASLAMGATRWQTIWRVILNAARPGIFTAI
                     VFGMARAFGEALAIQMVVGNSAILPTSLTTPAATLTSVLTMGIGNTVMGTVQNNVLWS
                     LALVLLIMSLAFNTVIKLITREGKKNYER"
     gene            9137..10024
                     /gene="pstA"
                     /locus_tag="ACUBV3_001086"
     CDS             9137..10024
                     /gene="pstA"
                     /locus_tag="ACUBV3_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000992189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HHU3847593.1"
                     /translation="MNAKKADKLATTILYSIAAIIVTILASLLIFILVRGLPHVSWSF
                     LTGKSSSYEAGGGIGIQLYNSFFLLIVTLIISIPLSLGAGIYLSEYAKKGRLTNFVRT
                     CIEILSSLPSVVVGLFGYLIFVVQFQYGFSIISGALALTVFNLPQMTRSVEDSLQNVH
                     HTQREAGLALGISRWETVIYVVVPEALPSIVTGVVLASGRIFGEAAALIYTAGQSAPA
                     LDWSNWNVLSVTSPISIFRQAETLAVHIWKVNSEGTIPDATQVSAGSAAVLLVVILIF
                     NLSARSIGKKLHSKLTSSN"
     gene            10036..10839
                     /gene="pstB"
                     /locus_tag="ACUBV3_001087"
     CDS             10036..10839
                     /gene="pstB"
                     /locus_tag="ACUBV3_001087"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HHU3847594.1"
                     /translation="MAEYNWDERHIITFPEENSALTTKDLHVYYGEKEAIKGIDMQFE
                     KNKITALIGPSGCGKSTYLRSLNRMNDTIDIARVTGQIMYEGIDVNAQDINVYEMRKH
                     IGMVFQRPNPFAKSIYKNITFAYERAGVKDKKFLDEVVETSLKQAALWDQVKDDLHKS
                     AFTLSGGQQQRLCIARAIAVKPEILLMDEPASALDPIATMQLEETMFELKKNYTIIIV
                     THNMQQAARASDYTAFFYLGDLIEYDKTNNIFQNAKCQSTSDYVSGRFG"
     gene            10851..11609
                     /gene="pstB"
                     /locus_tag="ACUBV3_001088"
     CDS             10851..11609
                     /gene="pstB"
                     /locus_tag="ACUBV3_001088"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HHU3847595.1"
                     /translation="MTQPILQVSDLSVYYNKKKALKEVSMDFYPNEITALIGPSGSGK
                     STLLRAINRMGDLNPEVTLTGAVMYNGHNVYSPRTDTVELRKEIGMVFQQPNPFPMSV
                     FENVVYGLRLKGIKDKATLDEAVETSLKGASIWDEVKDRLHDSALGLSGGQQQRVCIA
                     RTLATKPKIILLDEPTSALDPISAGKIEETLHGLKDQYTMLLVTRSMQQASRISDRTG
                     FFLDGNLIEYGNTKEMFMNPKHKETEDYITGKFG"
     gene            11643..12296
                     /gene="phoU"
                     /locus_tag="ACUBV3_001089"
     CDS             11643..12296
                     /gene="phoU"
                     /locus_tag="ACUBV3_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HHU3847596.1"
                     /translation="MLRSKFDEELDKLHNQFYAMGIEAIGQIKKTVRAFVSHDRELAK
                     EVIEDDVTLNNFETKLEKKSLEIIALQQPVSQDLRTVITVLKATSDVERMGDHAAAVA
                     KATIRMKGEERIPAVELEINNMGKAVKNMLEEALTAYINGDDEKAYEVAAMDEIVDDY
                     FRDIQKMVVETIQKHPDVAFAAKEYFQVLMHLERIGDYGKNICEWIVYLKTGKIIEL"
     gene            12442..>14990
                     /locus_tag="ACUBV3_001090"
     CDS             12442..>14990
                     /locus_tag="ACUBV3_001090"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HHU3847597.1"
                     /translation="MKTVEHFVTKFVPENYNLFLDINRQTKTFSGNVAVSGEALDNNI
                     SFHQKGLTIKSVLLDNQPLDFQLDEDNEAMHIQLHETGSMVLVFEFSGHITDNMTGMY
                     PSYYTVNGIKKEVISTQFESHFAREVFPSIDEPEAKATFDLSLKFDQKEGEIALSNMP
                     EINAEQRQETGLWTFDTTPKMSSYLLAFALGELHGKTTHTKNGTLVGSYATKAHQLNE
                     LDFSLDIVVRVIEFYEDYFGVRYPIPQSLHVALPDFSAGAMENWGLVTYREVYLLVDE
                     NSSVSSRQQVALVVAHEIAHQWFGNLVTMKWWDDLWLNESFANMMEYVSIDYIEPKLN
                     IFEDFQTGGLPLALKRDATDGVQSVHVEVNHPDEINTLFDPAIVYAKGSRLMHMLRRW
                     LGDTDFAAGLKIYFEKHQYQNTIGRDLWNALSQTSGKDVAAFMDSWLEQPGYPVMAAK
                     IEEDELILTQKQFFIGEHEDKSRLWQIPLNSNWEGIPEILTEETVVIPNFSQLAEKNK
                     ENGALRFNTENTAHYITNYQGQLLEHIISDLPLMDNISKLQIVQERHLLAESGMISYS
                     SLIPLVSLLSQETSYLVNSAIKSVIDGLSLFVQEDSQDEFDFKEFVNKLSAFNFNRLG
                     FEKREGEGDDSEMVRHLSLSLALYSDNEHAIEEAHHIFKAHENNIAAIPAAIRLLVLT
                     NEMKHFESKELSHLLLETYSTTTDGNFKRQLASALSHTTDSKTLKKLLSDWKNKDIVK
                     PQDLAMSWYATFLKNSFTQESVWEWAQENWEWIKATLGGDMSFDKFVIYPSSSFKTEE
                     RLEQYKNFFEPQLSDMAISRNISMGIKEISARVLLITKQKEEVINTIKKYI"
BASE COUNT         5146 a         2549 c         2777 g         4518 t
ORIGIN      
        1 acgaaaggag tctattgtgc cacaaaatcc aacaagtaac ccagagaaaa aaacttattt
       61 attatctgaa ttggtagact gctttaaagg taaagctgtg ccttcaaaag cagaagcagg
      121 tgatatccgt attattaatt tgagtgatat gtctccttta ggaattgact accataactt
      181 aagaacattc caagatgaac aacgatcgct tttaaaatat ctacttcagg aaggcgatgt
      241 tttaatcgct tcaaaaggaa ctgtcaagaa agttgctatt tttgaagagc aagattaccc
      301 tgtcgttgcc tctgctaata ttacaatact aaggcctaca caacacatta gagggtacta
      361 cttaaagctt ttttttgatt cagaagaagg tcaacaggca cttgagaatg ctaacaaagg
      421 aaaagctgtg atgaatatta gcacaaagga actgttaaat attgcaatac caagtatccc
      481 gctttttaga caggattatc ttatccaacg ttataaacag gggctcaacg attacaaacg
      541 taagatagca cgtgcagagc aggagtggga acgtatccaa aatgacatca gacaacaact
      601 tagctagaac caaatacaaa acatcatagg aagagttatc atgaacgaaa tcaaatgccc
      661 tcattgtgga acagcttttg ccatcaacga gtctgaatac catcaattac tagaacaaat
      721 tcgtggagat gcttttgaca aagaagtaag tgaacggttg gaaaaagaac gtctaatatt
      781 aggggagcaa gcaaaaaatc aattacagga agttgttgta gaaaaagaca aggagatagc
      841 taaacttcag tacaaagtca aacaatttct tatagaaaaa gacaatcttc tcaaagacaa
      901 tgagtaccaa ctcgctgagc aattaaatca aaaagacatg atgcttcgcg accttgaaaa
      961 ccaaatcgat agactacgtt tagagcatga aaatagcttg caagaggcgc taacaaaagt
     1021 cgaacgagaa agagatgcaa tacaaaatca gttgcacatt caagaaaaag aaaaagattt
     1081 agctttagct tcagtaaaaa gtgattatga agtacaacta aaggcagcca atgaacaagt
     1141 agaattctat aaaaacttca aagctcaaca gtctactaaa gcagtaggag aaagtttaga
     1201 acattatgct gaaacagaat ttaataaagt gcgacatttg gcctttccta atgcttattt
     1261 tgagaaggac aatacattat caagtcgtgg ctcaaagggg gactttatct atcgagaaaa
     1321 ggatgaaaat gaccttgagt ttttaagtat catgtttgaa atgaaaaatg agtctgatga
     1381 tactatcaag aagcataaaa atgaagattt tttcaaagaa ttagataaag atcgtcgtga
     1441 aaaatcttgc gaatacgcag ttttagtaac tatgcttgaa gcagacaatg actattataa
     1501 tactggaatt gttgatgtta gtcacaaata ccctaaaatg tacgttatac gtccacaatt
     1561 ttttatccaa ttaattggta ttctaagaaa tgcagcactc aataccttaa aatataaaca
     1621 agagcttgct ttgatgaaag aacaaaatat tgacatcaca cattttgaag aagatttaga
     1681 tattttcaaa aatgcatttg ctaaaaatta taattctgca agcaaaaatt tccagaaagc
     1741 aatcgatgaa atagataaat ctattaaacg tatggaagct gttaaggctg ctttaacaac
     1801 gtctgaaaat caactacgtc ttgcaaataa taaattagac gatgtttctg tcaagaaatt
     1861 aacaagaaaa aatccaacaa tgaaagcaaa attcgatgct ctaaaagact aagaggttat
     1921 gattatgaag attagaactg ctacactaga cgatagtgaa aaattagtcc ctttgtatca
     1981 agaattagga tacgcaatat cattatcaga gattcaatct attttaaagg ttattcttac
     2041 tcattcagat tatggctttt tgatagctga ggataatggc aaactactcg ccttcgttgg
     2101 atatcataaa ctttactttt ttgaaaaaag tgggacttac tatcgtattt tagcactagt
     2161 agttaatgaa aagcaccgtc gtaaaggcat cgctagtcaa cttattaatc acgttaaaca
     2221 gctagctaaa acagatggaa gtgaagtact ggctttaaat agcagtttaa aagaatatcg
     2281 tcaggaagct tatcattttt atgaaaacct agggttcaag aaggttagta caggcttttc
     2341 atactattta gattaggtat aaatccttat agcactatac caaaagagcg taagttttta
     2401 cgctcctttc tttgtgttac cgcttattta ctgatataat ataaaacatg attacaggaa
     2461 taattaattt aaagaaagaa gctggaatga catcacacga tgctgtattc aagcttcgaa
     2521 agatattaca tacaaaaaaa ataggacacg gcggaactct tgatccagat gttgttgggg
     2581 ttttaccaat tgcggttgga aaagccactc gtgttattga atatatgaca gaaagtggca
     2641 aaatttatga aggagaaatc actttaggat atgccacaag tacagaagat tctagtggag
     2701 aggtcatttc aagaactcca ctaactcaat ccgatcttag cgaggatgtt gtggatcatg
     2761 cgatgaaaag ttttaccggt ccaattacac aagttcctcc tatgtattca gcagttaaag
     2821 taaatggtaa aaaactctat gaatatgctc gttcaggaga agaagtagag cgacctaaac
     2881 gacaaataac aatttctgaa tttagacgca caagcccatt gtacttcgaa aaaggcatct
     2941 gccgttttag tttctatgtc tcatgcagta aaggtaccta tgttaggacg ttagctgttg
     3001 atctaggaat aaaactagga tatgctagcc atatgtcttt cctaaaaaga acctcatctg
     3061 caggattatc tattacacaa tcattaacgc ttgaagaaat taatgaaaaa tataagcaag
     3121 aagatttttc atttttactt ccaattgaat atggtgtttt agatctccca aaagttaacc
     3181 taactgagga agataaagtt gaaatctcat acggacgtcg tatcttatta gaaaatgaag
     3241 cagatacctt agcagctttt tatgaaaata gagttatcgc tatactagaa aaacgtggca
     3301 atgaatttaa accacacaaa gtattgttat aaagaaagtc aatcatgaaa atccattaca
     3361 ttaatgatta taaagatatt caagctaaag aagattgcgt cttagttttg ggctattttg
     3421 atggcttaca tctgggacat aaagccctct ttgataaagc taaaaaaatc gcgaccgaaa
     3481 aaaatttgaa aattgtcgtc ttaacgttta atgagacacc tcgtctaacc ttcgctcgct
     3541 tccaacctga attattgcta catcttacct ctccagaaaa acgttctgaa aaatttcaag
     3601 agtatggcgt cgatgagtta tatttaatga attttaccag tcatttttca aaagtatctt
     3661 ctgacctttt tattaagaaa tatatttatg gtctcagagc aaaagctgct gttgtcggtt
     3721 ttgattataa gttcggtcat aatcgtacca gtggtgatta tctagcgcgt aatttcaagg
     3781 gaccagtcta tattattgat gaaatttctg aaggtggtga aaaaatctct tctacacgta
     3841 ttcgtcaatt gattacagag ggtaatgttg agaaagctaa ccaacttttg ggttatgagt
     3901 tttcaacctg tggcatggta gtacatggag atgctagagg acgaactata gggttcccaa
     3961 ctgctaatct agctcctatt aatcgcactt acttacctgc agacggtgtt tatatttcca
     4021 atgttctgat taatggaaaa tattatagag caatgacgag cattggaaaa aatatcactt
     4081 ttggtggtac agaacttcgt ttagaagcta atatctttga ttttgatggc gacatttatg
     4141 gagaaactat tgaaattttc tggttaaaaa gaatcagaga aatggtaaag tttaatggta
     4201 ttgacgatct tgtaaaacaa cttaaaaaag ataaagaaat tgctttaaat tggaaaaaag
     4261 atagtcaaac tctttaattt tttgtataat agtctctagt aaatacaaga ggcggttata
     4321 tgattacctt atttttatca cccagctgta ctagttgtcg aaaagcgcgt gcttggctaa
     4381 gtaagcatga ggttgctttt gaagagcata acattattac aagtccactc aataaagaag
     4441 aactattaca aattctatcg tttacagaaa atggtacaga agacattatt tccacacgtt
     4501 caaaagtctt tcaaaaactt gctattgatg tagacgaatt atcaacgtct tcattaatgg
     4561 aattaatttc agaaaatcca agtcttttac gacgcccaat tattttagac aaaaaacgta
     4621 tgcagattgg ttttaatgag gatgaaattc gtgccttctt accgcgtgac tatcgtaaac
     4681 aagaactgaa acaggccact attcgtgctg aaattgaggg aaaacatgat tagtagtaat
     4741 tatagttatc ctcttgatcc ttcatggaat acggaagata ttactaaggt acttcgcttt
     4801 ttaaatcaag ttgaacacgc ctatgaaaat agtgttaaag ttgatgattt gctagatagc
     4861 tataaagaat ttaaaaaatt tgttaaatcc aaggcgcagg aaaaacaaat tgaccgtgaa
     4921 ttccagagaa ctagcggtta ttccacttat caagctgtta aagcagcaca acaacaagcg
     4981 aaaggattta tttcacttgg acgctaaatt tgatttcgct aaacaattag tgtataaagc
     5041 tggtcaattc attaaatctg agatgcaaaa tacttttgat gttgaggaaa agtctcgatt
     5101 tgatgattta gtgacaagtc ttgataaaaa aacacaaaaa ttattaattc aagaaattat
     5161 tcaacattac ccagatgata atatacttgc cgaagaagat gatgtgcgct cacccattgc
     5221 acaaggtaat gtttgggttt tagaccctat tgatggaact gttaatttta ttgttcaaaa
     5281 agataacttt gctgttatgt tagcttatta cgaagaaggc gttggacaat ttggtatcat
     5341 ctacgacgtt atggctgata ttctttacag cgggggagga cattttgatg tctatgctaa
     5401 cgataaaaaa attgttcctt ttcaagagtg tcctttagaa cgttgcttac ttggagtaaa
     5461 ttctgctatg tatgcagaaa atgattgcgg tattgcccat ttagcttctg aaaccttagg
     5521 tgttcgtatc tatggtggtg ctggaattag catggcaaaa gtcatgcagg gcaaattatt
     5581 agcttatttt tcgtatattc aaccttggga ttatgctgca gcaaaaatca tgggggagac
     5641 attaggattt accttattaa cgcttgacgg ggaagaaccc aactattcaa caagacaaaa
     5701 agtaatgttc ctaccaaagt cgaaattaaa tcttatccaa tcctacctta ccaaaaaata
     5761 aacttaaaac cttctaatta aatagaaggt ttttttgcac gcccgtgatt atgtagtata
     5821 atagaaacat gaaattaccg aatgaattta ttgaaaaata tcagactata cttaaagatg
     5881 aagcagaagc tttttttgac agttttgaac aaaaacccat atcggcatat cgcaccaatc
     5941 ctctcaaaga aaagcaactt gattttccaa atgccatccc aagtacccct tgggggcatt
     6001 atgggaaaat atcaggaaaa tcaatagaac acacaactgg ccttgtttat tctcaagaac
     6061 ctgctgctca gatagtggct cagattgctg aaccacaaga aggaatgaaa gtgctagatt
     6121 tagcagctgc acctggtgga aaaacaacac accttctatc atatttaaac aatactggtc
     6181 tattagttag taatgaaatt tcaaataaac gtagtaaaat tttagtcgaa aatgtcgaac
     6241 gttttggtgc tagaaatgtt atcgtgacta atgaaagctc ccaacgacta gccaaatgtt
     6301 ttaattcttt ttttgatctt attgtttttg acggtccgtg ttctggtgag ggaatgttcc
     6361 gcaaagatcc tcaagcaata caatattggc acaaagatta ccccactgaa tgtgcacagt
     6421 tacaaagaga tattttaaaa gaagcaatca aaatgctagc tcatggtggt atacttgtat
     6481 actctacttg tacatggtca ccagaagaga acgaagaagt ggttaattgg ctccttcaag
     6541 agtatgatta cttagaactt gttgatattc ctaaactaaa tgggatggtt gaagggatta
     6601 atgtaccaca agttgcaagg atgtaccctc atcattttca aggtgaagga caattcgttg
     6661 caaaactgag agatactaga tctaaggaag cacagaaaat taagccaaaa gcacagaaaa
     6721 taaataaaat gcagttacaa ttgtggcaac aatttgcaca agaccattta aagatagact
     6781 tgaacggtgt tttagatgtt ttcggcgacc aactctatct tctacctaat ggtctccctg
     6841 atttgtctaa attaaaaata gcacgcaatg gtctccatct aggcactttc aagaagaatc
     6901 gttttgagcc atcgtttgct ttaggaatgg cacttagcga acatgaccta gtacagtcta
     6961 ttgaaattga tatagaacag tttgaggtgt acgtatctgg aaatgtagtc aaactagaca
     7021 agactgttcc aaacggttgg tatcaaattc ttgtaaaagg caatggatta ggttttgcaa
     7081 aagtgacaaa taatactctt aaaaattatt accctaaagg actacgattt cagacataac
     7141 tgttattgaa tacttttaat aaaaaaggaa aaagaaatac ttttcaaacc ctagaaactt
     7201 atggtactat gggacagtga tgtgtgtttt tactatttgt aaatcattaa acactcaatg
     7261 attttagtaa tatatatcaa ctactaaata tcaaaattta ttcaaaagaa taggaaaatg
     7321 aaaatgaaaa aaaacatttt tttgttaacc ttgctagcca ccgtcttatt gacgctgtct
     7381 ggttgtgcta attggattga taaaggtcaa tcaatcacca gcgttggttc aaccgcctta
     7441 cagccacttg tagaagctgc agcagatgag tttggtaaga caaacctcgg taagactatc
     7501 aatgtccaag gaggaggctc aggaacaggc ttatcacaag tacagtctgg agctgttcaa
     7561 attggtaata gcgacttatt tgcagaagaa aaagatggta ttgatgctaa gaaattgaaa
     7621 gaccatcaag tcgcagtagc tggattagct gttattgtta ataaaaaagt taatgttaaa
     7681 aatctaacca cccaccaact tcgtgatatt tttgcaggaa aaatcaagaa ttggaaagaa
     7741 gtgggaggac aagatttaga tatttccatc atcaatcgag cagcaagttc tggatcccgt
     7801 gcaaccttcg ataatactat tatgggaaat gttgctccta ttcaaagcca agaacaagat
     7861 tcaaatggta tggtgaaatc aattgtttct caaacacctg gtgcgatttc atacctcgct
     7921 tttgcatacg ttgataaatc tgtcggaaca ttaaaactaa atggttttgc gccaacagct
     7981 aaaaatgtta ctaccgacaa ctggaaactt tggtcatatg aacatatgta tacaaaaggt
     8041 aatgaaacag gtctaacaaa agaattctta gactacatga aaagtgataa agttcaaagt
     8101 agtatcgtac aacatatggg atatatctct attaacgaca tgaaagttgt taaagatgct
     8161 gaaggtaaag taaccccaaa ataaaactta atcacatcta gataaagcat cttgatccta
     8221 ggaggaatta tgaaaaatca agaattagct aaaaaattaa catctccttc aaaaaactct
     8281 cgattagaaa aatttggtaa aggaataact tttctttcgc ttgccctaat cgtcttcatt
     8341 gttgcgatga ttttgatttt tgttgctcaa aaaggactat ctacattttt tgttgatggc
     8401 gttaaattaa cagacttctt atttaataca aaatgggaac caagtgctaa gtcattcggt
     8461 gctcttccaa tgatagctgg atcatttatt gttactattc tatcagctat cattgctaca
     8521 ccatttgcta tcggtgctgc cgtatttatg actgaaatct cacctaaata tggttctaaa
     8581 attttacaac ctgcagttga attactagtt gggattcctt ctgttgttta cggttttatt
     8641 ggtttacaga ttattgttcc cttcgttcgc tctatttttg ggggaacagg ttttggcatt
     8701 ttatctgggg tctgtgtcct cttcgtcatg attctaccaa ctgttacttt catgacagta
     8761 gatagcctta gagctgtgcc acgtcactat aaagaagcaa gtttagccat gggggcaact
     8821 cgttggcaaa caatttggcg tgttatatta aacgctgcac gtccaggaat tttcactgca
     8881 attgtttttg gtatggcacg tgcatttggt gaagctctag caattcagat ggttgtcgga
     8941 aactctgcaa ttttaccaac atcattgaca acaccagcag caacattaac gtctgttcta
     9001 acaatgggta ttggtaatac tgtaatggga actgtacaaa acaatgtcct ctggtcgcta
     9061 gccttagtac ttttaattat gagtttagca tttaatacag ttattaagtt aattacaagg
     9121 gaaggaaaga aaaactatga acgctaaaaa ggctgacaaa ttagcaacta caatactcta
     9181 tagtattgca gctatcatcg ttacaatcct cgcctctctt ttaattttta ttcttgttcg
     9241 cggattgcca cacgtatctt ggtcattttt aactgggaaa tcatcatcat atgaagcagg
     9301 aggcggtatt ggaatacaat tatacaattc ctttttccta cttattgtta ctctgattat
     9361 ttctattcct ctatctttag gagctggaat atacctctca gaatatgcta aaaaaggacg
     9421 tttaacaaac tttgttagaa catgtatcga aattttgtca tctcttcctt ctgttgtagt
     9481 tggtctgttt ggttacctaa tcttcgttgt tcaatttcaa tatggcttct caattatctc
     9541 aggtgcctta gcacttactg tttttaactt accccaaatg acacgtagtg ttgaagatag
     9601 ccttcaaaat gtacaccaca cacagcgtga agcaggattg gccctaggta tttctcgttg
     9661 ggaaacggtc atttacgtcg ttgtccctga agctctgcca agtattgtaa ctggtgttgt
     9721 gctagcttca ggacgtattt tcggtgaggc agcagcactt atttatacag ctggtcaatc
     9781 tgctccagca cttgattggt caaattggaa cgtccttagt gttacaagtc caatctcaat
     9841 tttccgtcaa gcagaaactt tagctgttca tatttggaaa gttaatagcg aagggacaat
     9901 ccctgatgca acacaagtat ctgccggtag cgccgcagtc ttattggttg ttattcttat
     9961 tttcaacctt tcagctcgct cgatcggtaa gaaacttcat tcgaaattaa cctcgtctaa
    10021 ttaaaaggag tagtaatggc agaatataat tgggacgaac gccatatcat taccttccct
    10081 gaagaaaatt cagctctcac cacaaaagat ttacacgttt actacggtga gaaagaagct
    10141 atcaagggaa ttgatatgca atttgaaaaa aataaaatta cagccttaat cggcccatct
    10201 ggatgtggaa aatcaacata tcttcgtagc ttaaatcgta tgaacgatac gattgatatt
    10261 gcacgcgtga ctggacaaat catgtatgaa ggaattgatg ttaacgctca agatatcaat
    10321 gtttacgaga tgcgaaaaca tatcggaatg gttttccaac gtccaaaccc gtttgcaaaa
    10381 tcaatttata aaaacattac ctttgcatat gagcgtgctg gtgtaaaaga taaaaaattc
    10441 ttagatgaag ttgtcgaaac ctcactaaaa caagctgctc tatgggatca agtaaaagat
    10501 gaccttcata aatcagcatt cactttatct ggcggtcaac agcagcgatt atgtattgca
    10561 cgtgctatcg cagttaaacc agaaattctc ttaatggatg aacctgcttc agcactcgat
    10621 ccgattgcaa ctatgcaact agaagagaca atgtttgaac tcaagaaaaa ttacactata
    10681 attatcgtaa cacataatat gcaacaagca gcacgtgcta gtgattatac tgcatttttc
    10741 tatcttggag acttaatcga atacgataaa acgaataaca ttttccaaaa tgctaaatgt
    10801 caatctacta gcgattatgt ttctggtcga tttggatagt gaggtaaaag atgacacaac
    10861 ctattttaca agtcagtgat ttgtctgttt actataataa gaaaaaagct ttaaaagaag
    10921 tctcaatgga cttctatcct aatgaaatta cagccttaat tggtccgtct ggttctggaa
    10981 agtcaacact cttacgagct attaaccgta tgggagactt aaatcctgaa gtcactctaa
    11041 caggagcagt gatgtataac ggacataatg tttatagccc gcgcactgat accgtagaac
    11101 ttcgtaaaga aattgggatg gtttttcagc agcctaatcc atttcctatg tcagtatttg
    11161 aaaatgttgt ttatggtctt agactcaaag gaataaaaga caaagctacg ttagatgaag
    11221 ccgtagagac ttcattgaag ggcgcgtcta tctgggacga ggtcaaagat agattacacg
    11281 actctgctct tggtttatct ggtggtcaac aacaacgtgt ttgtattgcc agaacccttg
    11341 ctaccaagcc taaaattatc cttctagatg agccaacctc tgcacttgac ccaatttctg
    11401 ctggtaaaat tgaagagact ttacatggtc taaaagatca atataccatg ttacttgtta
    11461 cacgttctat gcagcaagcc tcacgtattt ctgatcgtac aggtttcttc ttagacggta
    11521 acttaattga atatggcaat acaaaagaaa tgtttatgaa tcctaaacat aaagaaactg
    11581 aagattacat tacaggtaaa ttcggttaat accatataat aagatcagaa agagaataca
    11641 atatgttaag atcaaaattt gatgaagaat tagataaact gcataaccaa ttctatgcaa
    11701 tgggaattga agctatcgga caaatcaaaa aaacagttcg tgcttttgtc agtcatgatc
    11761 gagagttagc taaagaagtg atagaagatg atgtaacgct taacaacttt gaaacgaagt
    11821 tagagaaaaa atcattggaa attattgcac tccagcagcc tgtatcacaa gacttacgca
    11881 ccgttattac cgttttaaaa gctactagtg atgttgagcg tatgggagac catgcagcag
    11941 cagttgcaaa agcaaccatc cgtatgaagg gcgaggaaag aatcccagca gtagaattag
    12001 aaatcaataa tatgggaaaa gcagttaaaa acatgcttga agaagcctta acagcttata
    12061 tcaatggtga tgatgaaaaa gcttacgaag tagcagcaat ggatgaaata gttgatgatt
    12121 acttccgtga tatccaaaaa atggtagttg aaacaattca aaagcatcct gacgtagcat
    12181 ttgccgccaa agaatacttc caagtattga tgcacttgga gcgaattgga gattatggca
    12241 aaaatatctg tgaatggatt gtttatttaa aaactggaaa aattatcgag ttatgatata
    12301 attattgctt gtagagtggt aattgtaagt atagttcgtg cgatagaaaa gctgtaacta
    12361 tcttgttaca gcttttctat cttttctagc ccatacagga ctagtaatgt ttcaaatgtt
    12421 tctgaattag ttaggagtta tatgaaaact gtagaacatt ttgttaccaa atttgtccct
    12481 gaaaactata accttttctt ggatattaat cgtcaaacta aaaccttttc aggtaacgtt
    12541 gctgtgtctg gcgaagcctt agataacaat atctcttttc accaaaaagg gttaaccatt
    12601 aaatctgtct tattagataa tcaaccatta gatttccaac tagatgaaga caatgaagct
    12661 atgcatattc aattgcatga aactggtagc atggtattag tctttgaatt cagcggtcac
    12721 attaccgata atatgacagg tatgtatcca tcttactata cagttaatgg catcaaaaaa
    12781 gaggttatct ccacacaatt tgaaagtcat tttgctcgtg aagtattccc aagtattgac
    12841 gaaccagaag caaaagcaac ctttgatttg agcctaaaat tcgatcaaaa agaaggagag
    12901 attgctcttt caaatatgcc ggaaatcaat gctgaacaac gtcaagaaac aggtttatgg
    12961 acatttgaca ccacacctaa aatgtcctca tatttattag cttttgcctt gggggaattg
    13021 catggtaaaa caacacatac caaaaacggc actcttgttg gctcatatgc cacaaaagcc
    13081 catcagttaa atgaattaga tttttcttta gatatcgttg tgcgtgttat tgagttttat
    13141 gaggactact ttggtgtacg ttatcctatt cctcagtcac tccacgttgc tctacctgat
    13201 ttttctgctg gagctatgga aaattgggga ttagtaacat atcgtgaagt ctatctttta
    13261 gtagatgaga actctagtgt ttccagccgt cagcaagtag cattagttgt agcacatgaa
    13321 atagctcacc agtggtttgg taacctcgtt actatgaaat ggtgggatga tttatggtta
    13381 aatgaatcat ttgctaatat gatggaatat gtatcaattg attatattga accaaaactt
    13441 aatatatttg aagactttca aacaggtggt ctaccattag ctcttaagcg tgatgccaca
    13501 gatggcgtcc aatctgttca cgttgaggtc aatcatcctg atgaaattaa cacacttttt
    13561 gatccagcta tcgtttatgc aaaaggtagc cgcctcatgc acatgttacg tcgctggtta
    13621 ggcgatacag attttgccgc aggactaaaa atttattttg aaaaacatca atatcaaaat
    13681 actattggtc gtgacctttg gaatgcactt tctcaaacct caggaaaaga tgttgcagct
    13741 ttcatggatt cttggttaga gcaaccagga taccctgtga tggcagccaa aatagaagaa
    13801 gatgagctca ttttaactca aaaacaattc tttattggtg aacacgaaga taagtctcgc
    13861 ttgtggcaaa ttcctcttaa tagtaattgg gaaggaatcc cagagatatt aaccgaggaa
    13921 acggtagtaa tccccaattt tagtcagtta gctgaaaaga acaaagaaaa tggtgcactg
    13981 cgtttcaata ctgaaaatac tgcacattac attactaact atcaaggtca actcttagag
    14041 catatcattt ctgatcttcc tcttatggat aacatttcta aactacaaat tgtacaagaa
    14101 cgtcatttac ttgctgaaag tggtatgatt tcatatagta gtttaatacc tttagtctct
    14161 cttttgagcc aagagacatc atatcttgtt aactctgcta ttaagagtgt tattgatggt
    14221 ttatccttat tcgtccagga agatagccaa gatgaattcg actttaaaga atttgttaat
    14281 aaattatcag catttaattt taatagactt ggctttgaaa aaagagaagg agaaggagat
    14341 gatagcgaaa tggtccgtca tctatcccta agccttgctc tctactcaga taatgaacat
    14401 gctattgaag aagctcacca tatttttaaa gcccacgaaa ataatatcgc tgctatacca
    14461 gctgcaattc gcctgttagt tcttaccaat gaaatgaagc atttcgaatc caaagaactt
    14521 agccacttgt tattagagac ttatagtact actaccgatg ggaatttcaa acgacaacta
    14581 gcgagtgctt tatctcatac tacagatagc aagacactca agaaattatt gtcagattgg
    14641 aaaaataaag atattgtgaa accacaagac ttagctatga gttggtatgc tactttcctt
    14701 aaaaattctt ttacacaaga aagtgtctgg gagtgggccc aagaaaactg ggaatggatc
    14761 aaagcaactc taggtggtga tatgagtttt gataaatttg tgatttatcc ctcaagtagt
    14821 ttcaaaacag aagaacgcct tgaacaatat aaaaactttt tcgagcctca attaagcgat
    14881 atggcgatta gccgtaatat ttcaatggga attaaagaaa tttctgctcg tgtcctttta
    14941 atcacgaagc aaaaagaaga agttatcaat actatcaaaa aatatatcta
//
LOCUS       DBJYSH010000046        14946 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.046, whole genome shotgun sequence.
ACCESSION   DBJYSH010000046 DBJYSH010000000
VERSION     DBJYSH010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 14946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14946
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.046"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            96..1028
                     /locus_tag="ACUBV3_001091"
     CDS             96..1028
                     /locus_tag="ACUBV3_001091"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HHU3847598.1"
                     /translation="MVSLKTEIAGFSFDNCLMNAAGIYCMTKEELLAIENSEAGSFVT
                     KTGTLEAREGNPQPRYADTDWGSINSMGLPNKGIDYYLDFVTELQDQDNSKNHVLSLV
                     GLSPEETHIILKKVENSSYNGLIELNLSCPNVPGKPQIAYDFEMTDLILSEIFSYYQK
                     PLGIKLPPYFDIVHFDQAATIFNKYPLAFINCVNSIGNGLVIDDETVVIKPKNGFGGI
                     GGDFIKPTALANVHAFYKRLNPSIKIIGTGGVKNGRDAFEHILCGASMVQIGTALQKE
                     GPEIFQRVSRELKEIMADKGYQSLEDFRGQLNYL"
     gene            complement(1072..1269)
                     /locus_tag="ACUBV3_001092"
     CDS             complement(1072..1269)
                     /locus_tag="ACUBV3_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3847599.1"
                     /translation="MSEKAILLIILIAFVIIAIIKIALLKLYMNTIKPKVDNEGNIIT
                     KPVKDDLTPEEREEREFDKDW"
     gene            complement(1466..1741)
                     /locus_tag="ACUBV3_001093"
     CDS             complement(1466..1741)
                     /locus_tag="ACUBV3_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HHU3847600.1"
                     /translation="MANKQDLIAKVAEATELTKKDSAAAVDAVFAAVADYLAEGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGAEIEIAASKVPAFKAGKALKDAVK"
     gene            complement(1844..2446)
                     /locus_tag="ACUBV3_001094"
     CDS             complement(1844..2446)
                     /locus_tag="ACUBV3_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HHU3847601.1"
                     /translation="MKQEKTGRNLNFWKWAFLLLLAINLSFTAVIASRLIQVREPNTG
                     KISTGVQDKVKVGTFTTNKSQLNKTIALYLKQYQTKKMNYKIYAASSSILFEGSYQLL
                     GYEVPLYIYFEPYRLTNGAVQLKVTSFSVGTLPLPEKDVLQYIKSSYKLPNFVDIKPK
                     KSVININLQDLKNKEGIYLKATAIDLVNDNFSFDIFKKKP"
     gene            complement(2421..3260)
                     /locus_tag="ACUBV3_001095"
     CDS             complement(2421..3260)
                     /locus_tag="ACUBV3_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HHU3847602.1"
                     /translation="MNKKKILTGLSFFLVSLLLSFGIFSLIIPKSNPKLTKKDFLTKK
                     VIPLNYVALGDSLTEGVGDTTSQGGFVPLLSESLHNRYSYQVTSVNYGVSGNTSQQIL
                     KRMTTDPQIEKDLEKADLLTLTVGGNDVLAVIRKELSHLSLNSFEKPAEAYKERLKEI
                     LAKARQDNPKLPIYVLGIYNPFYLNFPQLTKMQTVIDNWNKATKEVVDASENVYFVPI
                     NDRLYKGINGKEGITESSNSQASITNDALFTGDHFHPNNIGYQIMSNAVMEKINETRK
                     NWP"
     gene            complement(3253..4089)
                     /locus_tag="ACUBV3_001096"
     CDS             complement(3253..4089)
                     /locus_tag="ACUBV3_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HHU3847603.1"
                     /translation="MSKIKIVTDSSITIEPELIKELDITVVPLSVMIDGTLYSDNDLK
                     AQGEFLNLMRGSKELPKTSQPPVGVFAEIYEKLMNEGAEHIIAIHLTHTLSGTIEASR
                     QGANIAGADVTVIDSTFTDQCQKFQVVEAAKLAKEGADLDTILARVEEVRQKSELFIG
                     VSTLENLVKGGRIGRVTGLLSSLLNIKVIMELTNHELVPIVKGRGLKTFSKWLDNFVE
                     SAQTRKIAEIGISYCGKADMANNFKEKLAVLGAPISVLETGSIIQTHTGEDAFAVMVR
                     YE"
     gene            complement(4202..5860)
                     /gene="recN"
                     /locus_tag="ACUBV3_001097"
     CDS             complement(4202..5860)
                     /gene="recN"
                     /locus_tag="ACUBV3_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000923612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HHU3847604.1"
                     /translation="MLLEISIKNFAIIEEISLNFETGMTVLTGETGAGKSIIIDAMNM
                     MLGARASVEVIRHGANKAEIEGFFSVEKNQSLVQLLEENGIELADELIIRREIFQNGR
                     SVSRINGQMVNLSTLKAVGHYLVDIHGQHDQEELMKPNMHILMLDEFGNTEFNVIKER
                     YQSLFDAYRQLRKRVLDKQKNEQENKSRIEMLEFQIAEIESVALKSDEDQTLLKQRDK
                     LMNHKNIADTLTNAYLMLDNEEFSSLSNVRSAMNDLMALEEFDREYKDLSTNLSEAYY
                     VIEEVTKRLGDVIDDLDFDAGLLQEIENRLDVINTITRKYGGDVNDVLDYFDNITKEY
                     SLLTGSEESSDALEKELKILEHDLIESANQLSLERHKLAKQLENEIKQELTELYMEKA
                     DFQVQFTKGKFNKEGNEIVEFYISTNPGEGFKPLVKVASGGELSRLMLAIKSAFSRKE
                     DKTSIVFDEVDTGVSGRVAQAIAQKIHKIGSHGQVLAISHLAQVIAIADYQYFIEKIS
                     SDSSTVSTVRLLSYEERVEEIAKMLAGNNVTDTARTQAKELLGS"
     gene            complement(5872..6345)
                     /locus_tag="ACUBV3_001098"
     CDS             complement(5872..6345)
                     /locus_tag="ACUBV3_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HHU3847605.1"
                     /translation="MKKSERLNLIKQIVLNHAVETQHELLRRLEAYGVTLTQATISRD
                     MNEIGIIKVPSAKGRYIYGLSNENDPIFTTAVAKPIKTSILSISDKLLGLEQFININV
                     IPGNSQLIKTFIMSHCQEHIFSLTADDNSLLLIAKSEADADHIRQSMIAMLEKKD"
     gene            complement(6332..7159)
                     /locus_tag="ACUBV3_001099"
     CDS             complement(6332..7159)
                     /locus_tag="ACUBV3_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HHU3847606.1"
                     /translation="MAKERVDVLAYKQGLFDTREQAKRGVMAGLVINVINGERYDKPG
                     EKVADDTELKLKGEKLKYVSRGGLKLEKALQVFEISVADKLTIDIGASTGGFTDVMLQ
                     SGARLVYAVDVGTNQLVWKLRQDHRVRSMEQYNFRYAQKEDFKEGLPEFASIDVSFIS
                     LNLILPALKEILVDGGQVVALIKPQFEAGREQIGKNGIVKDKLVHEKVLTTVTNFTKD
                     YGYTVKHLDFSPVQGGHGNIEFLMHLQKCQDPQNLVLDQIQDVIEKAHKEFKKNEEE"
     gene            complement(7152..8024)
                     /locus_tag="ACUBV3_001100"
     CDS             complement(7152..8024)
                     /locus_tag="ACUBV3_001100"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HHU3847607.1"
                     /translation="MVTIEKIDEAIHRYYKQTHSVVSPDLIKAILYSVDGGGKRIRPR
                     ILLEILEGFGVELIDGHYDVAAALEMIHTGSLIHDDLPAMDNDDFRRGRLTNHKKFDE
                     ATAVLAGDSLFLDPFDLVVKAGFKADVTVRLIELLSMSAGSFGMVGGQMLDMKGENKV
                     LSIDDLSLIHINKTGRLLAYPFVAAGILAEKSEEVKGKLHQAGLLIGHAFQVRDDILD
                     VTASFEELGKTPNKDIVAEKTTYPNLLGLDKSQEILDDTLKKAQAIFQNLEKKANFNA
                     RKIIDIIEGLRLNG"
     gene            complement(8024..8239)
                     /locus_tag="ACUBV3_001101"
     CDS             complement(8024..8239)
                     /locus_tag="ACUBV3_001101"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HHU3847608.1"
                     /translation="MSDKKTFEENLQELETIVSRLETGDVALEDAIAEFQKGMLISKE
                     LQKTLKEAEETLVKVMQADGTEVEMDT"
     gene            complement(8217..9557)
                     /gene="xseA"
                     /locus_tag="ACUBV3_001102"
     CDS             complement(8217..9557)
                     /gene="xseA"
                     /locus_tag="ACUBV3_001102"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001286947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HHU3847609.1"
                     /translation="MSDYLSVSTLTKYLKLKFDKDPYLERVYLTGQVSNFRRRPNHQY
                     FSLKDDKSVIQATMWSGQFKKLGFELEEGMKVNVVGRVQLYEPSGSYSIIVEKAEPDG
                     IGALAIQFEQLKKKLSQAGYFDDRHKQLIPQFVRKIGVVTSPSGAVIRDIITTVSRRF
                     PGVEILLFPTKVQGEGAAQEIAQTIALANEKKDLDLLIVGRGGGSIEDLWAFNEECVV
                     EAIFESRLPVISSVGHETDTTLADFVADRRAATPTAAAELATPVTKIDILSWITEREN
                     RMYQSSLRLIMTKEERLQKSKQSVIFRQPERLYDGFLQKLDNLNQQLTFSMRDKLQTV
                     RQKQGLLHQKLQGIDLKQRIHTYQERVVQSRRLLSSTMTSQYDSKLARFEKAQDALIS
                     LDSSRIVARGYAIIEKNHTLVSTTNGINEGDHLQVKMQDGLLEVEVKDVRQENI"
     gene            complement(9683..10519)
                     /locus_tag="ACUBV3_001103"
     CDS             complement(9683..10519)
                     /locus_tag="ACUBV3_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HHU3847610.1"
                     /translation="MIVGEQEARALIKPRPKSSHKGDYGSVLLIGGFYPYGGAIIMAA
                     LACVKTGAGLVTVATQSCNIPSLHSQLPEVMAFDSDDYKWLEKSIVQSDVIVIGPGLG
                     VSESSRKILNQTMEKIQSHQSVIIDGSALTLLSEGAFPQTKAKNLVLTPHQKEWERLS
                     GIAVSQQTKENTQTALKSFPKGTILVAKSSHTRIFQDLDEKEIIVGGPYQATGGMGDT
                     LCGMIAGMLAQFKEASPLDKVSVGVYLHSAIAQELSKEAYVVLPTTISDEIPKEMARL
                     SK"
     gene            complement(10516..11370)
                     /locus_tag="ACUBV3_001104"
     CDS             complement(10516..11370)
                     /locus_tag="ACUBV3_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HHU3847611.1"
                     /translation="MTELIDGKALSQKMQAELGRKVERLKEQHGIIPGLAVILVGDNP
                     ASQVYVRNKERSALEAGFKSETLRLSESISQEELIDIIHQYNEDKSIHGILVQLPLPQ
                     HINDKKIILAIDPKKDVDGFHPMNTGHLWSGRPMMVPCTPAGIMEMFREYHVDLEGKH
                     AVIIGRSNIVGKPMAQLLLDKNATVTLTHSRTRNLSEVTKEADILIVAIGQGHFVTKD
                     FVKEGAVVIDVGMNRDENGKLIGDVAFEQVAEVASMITPVPGGVGPMTITMLLEQTYQ
                     AALRSVSL"
     gene            complement(11509..13203)
                     /locus_tag="ACUBV3_001105"
     CDS             complement(11509..13203)
                     /locus_tag="ACUBV3_001105"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001106850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HHU3847612.1"
                     /translation="MNYKEIYQEWLENDSLGKDIKSDLEAIKGDESEIQDRFYKTLEF
                     GTAGLRGKLGAGTNRMNTYMVGKAAQALANTIIDHGPEAIARGIAVSYDVRYQSKEFA
                     ELTCSIMAANGIKSYIYKGIRPTPMCSYAIRALGCVSGVMVTASHNPQAYNGYKAYWK
                     EGSQILDDIADQIANHMDAITDYQQIKQIPFEEALASGLASYIDESIEEAYKKEVLGL
                     TINDTNIDKSVRVVYTPLNGVGNLPVREVLRRRGFENVYVVPEQEMPDPDFTTVGYPN
                     PEVPKAFAYSESLGKSVDADILLATDPDCDRVALEVKDSKGEYIFLNGNKIGALLSYY
                     IFSQRCALGNLPHHPVLVKSIVTGDLSKVIADKYNIETVETLTGFKNICGKANEYDIS
                     KDKTYLFGYEESIGFCYGTFVRDKDAVSASMMVVEMTAYYKERGQTLLDVLQTIYDEF
                     GYYNERQFSLELEGAEGQERISRIMEDFRQDPILQVGEMTLENSIDFKDGYKDFPKQN
                     CLKYYFNEGSWYALRPSGTEPKIKCYLYTIGCTEADSLSKLNAIESACRAKMNSTK"
     gene            complement(13490..14224)
                     /locus_tag="ACUBV3_001106"
     CDS             complement(13490..14224)
                     /locus_tag="ACUBV3_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019805208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HHU3847613.1"
                     /translation="MVEPIISIQGLHKSFGKNEVLKGIDLDIHQGEVVVIIGPSGSGK
                     STFLRTMNLLEVPTKGTVTFEGIDITDKKNDIFKMREKMGMVFQQFNLFPNMTVLENI
                     TLSPIKTKGLSKLDAQTKAYELLEKVGLKEKANAYPASLSGGQQQRIAIARGLAMNPD
                     VLLFDEPTSALDPEMVGEVLTVMQDLAKSGMTMVIVTHEMGFAREVADRVIFMDAGII
                     VEQGTPKEVFEQTKEIRTRDFLSKVL"
     gene            complement(14217..14909)
                     /locus_tag="ACUBV3_001107"
     CDS             complement(14217..14909)
                     /locus_tag="ACUBV3_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001011649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HHU3847614.1"
                     /translation="MNFSFLPQYWSYFNYGVMVTIMISTCVVFFGTIIGVLIALVKRT
                     NLHFLTILANFYVWVFRGTPMVVQIMIAFAWMHFNNLPTISFGVLDLDFTRLLPGIII
                     ISLNSGAYISEIVRAGIEAVPSGQIEAAYSLGIRPKNTLRYVILPQAFKNILPALGNE
                     FITIIKDSALLQTIGVMELWNGAQSVVTATYSPVAPLLFAAFYYLMLTTILSALLKQM
                     EKYLGKGVKIDG"
BASE COUNT         4653 a         3016 c         2267 g         5010 t
ORIGIN      
        1 ttatttagcg aaattttatt accaatatca ttattttctt gccgctctgt taatcaagtg
       61 ataaaatagt aaaaatttat cttcaggagg atattatggt atcgctaaaa acggaaattg
      121 ctggttttag ttttgacaat tgtttgatga atgcagcggg tatttattgt atgactaagg
      181 aagagttatt agctattgaa aattctgaag ctggttcctt tgttacaaaa acaggtaccc
      241 ttgaagctag agagggaaac cctcaaccac gttatgcaga tactgactgg ggatcaatta
      301 attctatggg actccctaat aaaggtattg actattatct tgatttcgtg actgaattac
      361 aagatcaaga caatagcaaa aatcacgtct tatcattagt aggtttatca ccagaagaga
      421 cacatattat tcttaaaaag gttgaaaaca gtagttacaa tggcctaata gagttaaatt
      481 tatcgtgccc aaatgttcct ggaaaacctc aaattgctta tgattttgaa atgactgacc
      541 tcatcctttc agaaatcttt agctattatc aaaagccctt gggtatcaaa ttaccacctt
      601 actttgatat cgttcacttt gatcaagcag ctacaatttt taacaaatat cctttagctt
      661 ttattaactg tgttaattct atcggaaatg gacttgttat tgatgatgaa actgtcgtta
      721 taaagcctaa aaatggcttt ggaggtatcg gtggtgattt tatcaagcct accgccttag
      781 ctaacgtcca tgctttctac aaacgtttaa atccttcaat taaaattatc ggaacaggtg
      841 gtgttaaaaa cggccgtgat gcctttgaac acattctatg tggagctagt atggtccaaa
      901 tcggaaccgc tttacaaaaa gaaggaccag aaatttttca gcgtgtaagc agagaattaa
      961 aagaaataat ggctgataaa ggctatcaat cattggagga ttttcgagga caacttaatt
     1021 acctttaaaa ataataaaag agcaaacaag gtttgctctt ttatattgat tttaccaatc
     1081 tttatcaaat tcacgttctt cacgttcctc gggtgttaaa tcatccttta caggctttgt
     1141 tatgatgttt ccttcattat ctactttagg ttttatcgta ttcatatata actttaacag
     1201 agctatcttt ataatagcga taataacaaa agcaataagg ataattaata gtatagcttt
     1261 ttctgacatt ctctttcgca acctctcttt taacttatct tttaggaatt ataacatcac
     1321 agttaagata attcaagggt atgctctaac acatttgaaa ctctaaattc ctttgagctc
     1381 tctagtcgtt tattgagtat tggatataaa aaagaacctt gtcgctatca ataaacattg
     1441 atatgacaag gtttttctag acgaattatt taacagcgtc tttaagagct ttaccagctt
     1501 taaatgctgg aacttttgat gctgcaattt cgatttctgc accagtttgt gggttacgac
     1561 ctttacgagc tgcacgttca cgaacttcaa agttaccaaa accgattaat tgtacttttt
     1621 caccttcagc aaggtaatct gctacagctg caaaaactgc atctactgct gctgctgaat
     1681 cttttttagt taattctgta gcttctgcta cttttgcaat taaatcttgt ttgttagcca
     1741 tgttaacaaa tcctccaaat aaattctaaa cttatacaac ataagtttta acagattata
     1801 tgatatctaa aaaaaggctt aaggtcaagt atttaacgtc attttagggc tttttcttaa
     1861 aaatatcgaa actaaaatta tcgtttacta aatcaattgc agtcgctttc aaataaatac
     1921 cttctttatt ttttaaatct tgcaaattaa tattaataac cgatttttta ggttttatat
     1981 ctacaaagtt tggcaattta taagatgatt ttatatattg taacacatct ttttctggaa
     2041 gcggaagagt tcctactgaa aatgaggtca ctttaagttg aacagcgcca tttgttaaac
     2101 gataaggttc aaaatagatg tataaaggca cttcataacc taataattga taagagcctt
     2161 caaatagtat agaagatgaa gcagcataaa tcttataatt catcttctta gtttgatatt
     2221 gttttaaata aagtgcaatt gtcttattca gttgcgactt attggtcgta aaagtaccta
     2281 cttttacttt atcttgtacc ccagtcgaaa tttttcctgt attaggctca cgtacttgaa
     2341 ttaagcgact tgcaatcact gctgtgaagg aaaggttaat tgccaacaat aagagaaatg
     2401 cccatttcca gaaatttaaa ttacggccag ttttttcttg tttcatttat tttctccata
     2461 acggcgttag acatgatttg atagccaata ttattgggat gaaaatggtc tccagtaaag
     2521 agagcatcat tagtgatact tgcctgacta tttgatgact ctgtaatacc ctctttacca
     2581 tttattccct tataaaggcg gtcattaatt gggacaaaat aaacattttc tgaagcatca
     2641 actacttctt ttgtagcttt attccaatta tcaataacgg tttgcatttt agttaattgt
     2701 ggaaagttta ggtaaaaagg attataaatg cctaaaacat aaataggcaa tttaggatta
     2761 tcttgtcttg cttttgcaag aatttctttc aaacgttcct tatatgcttc tgctggtttc
     2821 tcaaaggaat ttagtgataa atgactgagc tctttacgaa taacagccaa gacatcatta
     2881 ccaccaacag ttagcgtcaa taaatcagct ttctctaaat ctttttcgat ttgaggatct
     2941 gtcgtcatac gttttaaaat ttgttgacta gtattcccag acacaccata attaacagaa
     3001 gtcacttggt aagagtatcg attatggagt gattctgata gcagtgggac aaaaccacct
     3061 tgagaggttg tatcccccac accttcggtc agagaatctc caagagcaac atagttaagt
     3121 gggataactt tctttgttag gaagtctttt tttgttaatt taggatttga ttttggaatg
     3181 atcaaggaaa aaatcccaaa agataaaaga agacttacca gaaaaaaaga aagtccggta
     3241 agtatttttt tcttattcat aacgaaccat aacagcaaat gcatcctcac cagtatgagt
     3301 ttggatgatt gagccagttt ctaatacgga tataggagca cctagaacag ctagtttttc
     3361 tttaaagtta ttagccatat cagctttacc acagtaggag atgccaattt cagcaatttt
     3421 tcgtgtttgg gcactttcaa caaaattatc taaccatttg ctgaaggtct ttagaccgcg
     3481 acctttaaca atcggtacta attcatggtt tgttagctcc ataattactt ttatgttaag
     3541 caatgaactt agaagacccg ttacacgtcc aatacgacca ccttttacta aattttcaag
     3601 ggtcgatacg ccaataaata attctgactt ctggcgtact tcttccacac gagccaaaat
     3661 tgtatctaaa tcagctccct ctttagctaa tttcgcggct tctacaacct ggaatttttg
     3721 acactggtct gtaaaagtag aatcaataac tgtaacatct gcaccagcaa tattagctcc
     3781 ctggcgtgat gcttcaatag ttcctgatag tgtatgcgtt aaatgaattg caatgatatg
     3841 ttccgcacct tcattcatta atttttcata aatttctgca aaaacaccta caggtggttg
     3901 acttgttttt ggcaattcct tacttccacg cattaggttc aagaattccc cttgtgcttt
     3961 taagtcattg tctgaataca aggtaccatc tatcataact gacaagggaa caactgtaat
     4021 gtcaagctct ttaataagtt ctggttcaat ggtaatggaa gaatccgtta caattttgat
     4081 tttactcata tcctatccta ttctatactt taatatttca atacccttat tataacaaaa
     4141 tttaagtgta tcaacattaa tttctcctaa caatgttagc tttttaaaga cgttaccaaa
     4201 gctaactacc aagtaattct ttagcttgag tacgtgcagt gtctgttaca ttgtttcctg
     4261 ccaacatttt ggcgatttct tctacacgtt cttcatagct taaaagcctt accgttgaaa
     4321 ctgttgaaga gtcactggaa attttttcaa taaaatattg gtaatccgct attgcaataa
     4381 cctgagctaa atgagaaatt gctaaaactt gtccatgact accaatcttg tgaatttttt
     4441 gagcgatagc ctgggccacg cgaccagaaa ctcctgtatc cacctcatca aatacaatgg
     4501 aagttttatc ttccttacgt gaaaaagcag atttaatagc taacatcaat cgagataact
     4561 ctcctcctga agccactttt accaatggct taaagccctc tccgggattc gttgaaatat
     4621 aaaattcaac gatttcgttc ccttctttat taaattttcc ttttgtaaat tgaacttgga
     4681 aatcagcttt ctccatataa agctctgtta attcttgttt gatttcattt tccaattgtt
     4741 tagctagctt gtgacgctct aaacttaatt gatttgctga ttcaatcaaa tcgtgttcta
     4801 aaatctttaa ttctttttct aaagcatctg aggactcttc actacctgtc aatagactat
     4861 attctttagt aatgttatca aaatagtcta aaacatcatt cacatcaccg ccatattttc
     4921 gcgttattgt gttaataaca tctagtctat tttcaatttc ttgtagtaaa ccagcgtcaa
     4981 aatctaaatc atcgataacg tcacctaaac gtttagtaac ttcttcaata acgtagtaag
     5041 cttctgaaag attggtggaa agatctttat attctcgatc aaattcttct aaagccataa
     5101 ggtcattcat tgcagaacga acattcgata aacttgaaaa ctcttcgtta tctaacataa
     5161 gatatgcatt tgtcaaagta tctgcaatat tcttatggtt cattaattta tcacgttgct
     5221 tgagtagcgt ttggtcttca tctgatttaa gggctacaga ctcaatttct gctatttgaa
     5281 attctagcat ttcaatacgt gatttattct cttgttcatt tttttgctta tccaatacgc
     5341 gtttacgaag ctgacgataa gcatcaaaaa gactttgata acgttctttt atgacattaa
     5401 attctgtatt accaaattca tctaacatca gaatgtgcat gttaggtttc attagttcct
     5461 cttggtcatg ctgaccatga atgtctacca aataatgccc cacagctttt agtgttgaca
     5521 aattcaccat ttgcccatta attcgactaa cgctgcgccc attttgaaag atttctcggc
     5581 gaataattaa ttcatctgct aattcaatgc cattttcttc caataattgg actaatgatt
     5641 gatttttttc aacagagaaa aatccttcaa tttctgcttt gttagcacca tggcgaatca
     5701 cttcaacact agcacgggct cctaacatca tattcatagc atcaataatg atagatttcc
     5761 ctgcaccagt ctcaccagtt aaaacagtca ttcctgtttc aaaattaagt gaaatttctt
     5821 cgataatagc gaaattctta atagaaattt ctaaaagcat aaggtcctct actaatcttt
     5881 tttctccaac attgcaatca ttgattgacg aatgtgatca gcatctgctt ctgattttgc
     5941 aatcaaaagg agactattat cgtcagctgt caaactaaaa atatgttctt gacaatgtga
     6001 cattatgaag gttttaatta attgactgtt acctggtatg acattgatat tgataaattg
     6061 ctctaaacct agtagcttat ctgatattga taaaatactt gttttaatag gctttgccac
     6121 agcagttgta aagatagggt cgttttcatt tgacaaaccg taaatatagc gaccttttgc
     6181 tgatggcact tttataatgc caatttcatt catatcacgt gaaatagttg cttgagttag
     6241 agttacccca taagcttcca atctgcgtaa caactcatgt tgtgtttcaa cagcatggtt
     6301 aagaacaatt tgcttaatta aatttaaacg ttcactcttc ttcatttttc ttaaattcct
     6361 tatgtgcttt ttctataaca tcttgtattt ggtcaagcac aagattttgt ggatcttgac
     6421 acttttgcaa atgcattaaa aactcaatat ttccatgtcc accttgaacg ggcgaaaaat
     6481 caagatgttt aaccgtatat ccataatctt tcgtgaaatt ggtcactgtt gtcaaaacct
     6541 tttcatgaac caacttgtct ttgacaatac catttttacc aatttgctca cgacctgctt
     6601 caaattgtgg tttaattaat gccactactt gtccaccatc cactaaaatt tcttttagag
     6661 ctggtaaaat caaattaaga gagataaatg agacatctat cgatgcaaat tcaggcagtc
     6721 cctccttgaa atcttctttt tgggcatacc taaaattata ttgttccata gaacgaacac
     6781 gatgatcctg acgtaacttc caaactaatt gatttgttcc tacatctact gcgtaaacta
     6841 aacgcgctcc tgattgtagc ataacatcag taaaaccacc cgtagaggcg ccaatatcta
     6901 tagttagctt atctgcaact gaaatttcaa aaacttgtaa agctttttct aatttcaatc
     6961 cacctctact aacatatttt agtttttcac cttttagttt taattcagta tcgtctgcaa
     7021 ccttttcgcc tggtttatca taacgttctc cattgataac gttaatcacc agtcctgcca
     7081 taacaccacg tttcgcttgc tctcgtgtat caaaaagtcc ctgtttatag gcaagaacat
     7141 ctaccctctc tttagccatt caaccgtaat ccctctatta tatctattat ttttctagca
     7201 ttaaagttag cttttttctc tagattttga aaaattgcct gagctttttt caaagtatcg
     7261 tcaagtattt cctgtgactt atccaaaccc aataaatttg gataagttgt cttttctgct
     7321 acaatgtctt tatttggtgt cttacccaat tcttcaaaac tagcagtcac atctaaaata
     7381 tcatcacgta cttgaaaagc atgaccgatt aaaaggccag cttgatgcag ttttcctttt
     7441 acttcttccg acttctcagc taaaatacct gctgcaacaa agggataagc taacaggcgt
     7501 cccgttttat taatatgaat caagcttaaa tcgtcaatag ataaaacttt gttttctccc
     7561 ttcatatcta acatttgtcc accaaccatg ccaaaactac ccgcagacat agacaataac
     7621 tctattaacc taacagtaac atcagcttta aaaccagctt ttacaactaa atcaaatggg
     7681 tctaaaaaaa gggaatctcc agctaaaacc gctgtagctt catcaaactt tttatggttt
     7741 gttaagcggc cacgacgaaa atcatcatta tccatcgccg gtaaatcatc atgaattaaa
     7801 gagccggtgt gaatcatctc caaagcagca gccacatcat aatgaccatc tattagttca
     7861 actccaaatc cctctaaaat ttctaaaagg atgcgtggac gaatacgttt cccacctcca
     7921 tcaactgagt agaggatagc cttaatcaaa tcaggagaaa cgacactatg tgtctgtttg
     7981 taataacgat gaatagcttc atcaattttt tcaatagtaa ccatcaagta tccatttcta
     8041 cttccgttcc gtcagcttgc atcactttta caagcgtctc ttcagcttct tttaaagttt
     8101 tttgtaactc ttttgaaata agcattcctt tttgaaattc agcaatggca tcttctaagg
     8161 caacatcacc tgtttctaaa cgtgagacaa ttgtctctaa ttcctgtaaa ttttcttcaa
     8221 atgttttctt gtctgacatc cttcacctca acttctaaaa gaccatcttg catcttaact
     8281 tgcaaatgat ctccttcatt tattccatta gtagtagata ctaaagtatg gtttttttcg
     8341 ataatagcgt aaccacgcgc tacaattcta gaactatcca atgatataag cgcatcttga
     8401 gccttttcaa aacgtgctag cttgctgtca tactgactag tcattgtact tgataatagt
     8461 cgtctacttt gtacaacacg ctcttggtaa gtatgaatac gctgttttaa atctatgcct
     8521 tgcagttttt gatgaagcaa accttgcttt tgtcttactg tttgtagttt atcacgcata
     8581 gaaaatgtta actgctgatt taggttatct aatttttgaa ggaaaccatc atatagacgc
     8641 tctggttgcc taaaaataac tgattgcttt gatttttgca acctctcctc ttttgtcatg
     8701 ataagacgaa ggctagactg atacattcta ttttcacgtt cagtaatcca agacaagata
     8761 tcaattttag taaccggtgt agcaagctca gctgctgcag tcggagttgc tgctctgcga
     8821 tcggctacaa aatctgctaa agttgtatca gtttcatgtc cgacactaga aataacggga
     8881 agacgagatt caaaaatagc ttctacaaca cattcttcat taaatgccca taagtcctca
     8941 atagaacctc ccccacggcc aacaattaat agatccaaat cctttttctc gttagctaaa
     9001 gcaatagttt gtgctatctc ctgagctgcg ccctctcctt gaacttttgt ggggaaaagt
     9061 aatatttcca ctcctgggaa tcgacgagat actgttgtaa taatatcccg aataacggct
     9121 ccacttggac tagtcacaac tcctattttt ctaacaaatt gaggaattaa ttgcttatga
     9181 cgatcatcaa aataacctgc ttgagagagt ttcttcttta gttgttcaaa ttggatagca
     9241 agagctccta taccatccgg ttctgctttt tcaacaataa tagagtagga gccgcttggt
     9301 tcataaagtt gaacacggcc aactacatta accttcatcc cttcctctaa ttcaaaaccc
     9361 agttttttga attgacctga ccacattgtc gcctgaatga ctgatttatc atcttttaaa
     9421 gagaaatatt gatggtttgg gcgacgacga aaattagaca cttgcccagt taaataaacc
     9481 ctctctaaat aagggtcttt gtcaaatttt aattttaaat acttagtcag tgtcgacacg
     9541 gataaatagt ctgacatagc acctcctcat gagagtttta ttaacttcat tataccaaaa
     9601 aaacttaaaa aatacctatt tgctctacaa atagctcgat aaagcaaaag aagtgtcatg
     9661 ctaacacctc tctacttatt ttttatttag atagtctagc catttctttt ggaatctcat
     9721 cactaatcgt tgtcggtaga acaacgtagg cttccttaga taattcttga gcaattgccg
     9781 aatgtagata aactcccact gataccttat ctagaggaga ggcttcttta aattgcgcta
     9841 acatacctgc aatcatacca cacaaagtat cccccatccc tccagtcgcc tggtaaggac
     9901 ctcctactat aatttctttt tcgtctaaat cttgaaaaat acgcgtatgc gaactcttag
     9961 cgactaagat cgttcctttg ggaaaagact taagagcggt ttgggtattt tcttttgtct
    10021 gttgcgatac agcaatacct gacaatcgct cccattcttt ttgatgaggt gtcaacacta
    10081 aatttttagc ctttgtttgc ggaaacgcac cttctgataa cagagtcaag gctgatccat
    10141 caatgatgac actttgatgt gattgaatct tctccatggt ctggttcaag atttttcgag
    10201 atgattctga tactcctaat ccaggaccaa ttacaataac atcactttga acaattgatt
    10261 tttccaacca tttgtaatca tcactatcaa acgccattac ctctggcagt tgactatgca
    10321 aagaggggat attgcaactt tgggttgcta cagtaactaa tcctgcacca gttttgacac
    10381 aggccaaagc tgccattata atagcacctc catagggata aaaacctcct atcagaagaa
    10441 cactaccata atcaccttta tggcttgatt tagggcgtgg cttaattaac gccctcgctt
    10501 cttgttcacc aacaatcata gactcacact tctaagagct gcttgataag tttgttccaa
    10561 aagcattgta atcgtcatag gtcctacgcc cccaggaaca ggtgttatca tactagcaac
    10621 ttctgccact tgttcaaatg caacgtctcc aatcaattta ccattttcat cgcgattcat
    10681 accaacatca atcaccacag caccttcttt aacgaagtct tttgtaacaa agtgcccctg
    10741 accaatcgca acaataagga tatcagcctc ctttgttacc tcagaaaggt ttcgagttct
    10801 tgaatgtgtc aaagtaactg ttgcgttttt atcaaggaga agttgtgcca ttggcttacc
    10861 aacaatattg gaccttccga tgataacagc atgttttccc tctaaatcca catggtactc
    10921 tctaaacatc tccataatac cagctggcgt acaaggtacc atcatcggtc gtcctgacca
    10981 aagatgaccc gtattcattg ggtggaaacc atcaacatct ttcttaggat ctattgctaa
    11041 aataattttt ttatcgttaa tatgttgtgg taacggaagt tgaactaaaa taccatggat
    11101 acttttatct tcattgtatt gatggataat atcaattagt tcttcttgag aaatggattc
    11161 agataatcgt agagtctcac ttttaaaacc agcttctaat gcagagcgtt ctttatttcg
    11221 aacgtacact tggctagctg gattatcccc aacaaggatt acagctaaac caggtatgat
    11281 accatgctgt tcctttaacc tttcaacttt tcgccctaac tcagcctgca ttttttgtga
    11341 taaggcttta ccatctatta attcagtcat tgatactcct tcttcatccg ttttttctat
    11401 attatatcaa aaaatagcga ttatgctatt ttatatcgct attcattttg tatcaatttt
    11461 aatcaataga aaaggcaatg aatccctcat cgccttttta catatgactt atttagtact
    11521 attcatttta gcacgacaag ccgactcaat tgcattaagt ttcgataaac tatctgcttc
    11581 tgtacaacca atcgtataaa ggtaacattt tatcttaggt tccgtccctg acggccttaa
    11641 agcataccat gaaccctcat taaaataata ttttaaacaa ttttgctttg gaaaatcctt
    11701 ataaccatcc ttgaaatcaa tagaattctc caatgtcatc tcacctactt gtaatattgg
    11761 gtcctgtcta aaatcctcca taatacgact aatacgttct tgcccctcag caccctctaa
    11821 ctcaagagaa aattggcgct cgttgtaata gccaaattca tcgtaaatgg tttgcaaaac
    11881 gtctaaaagt gtttgccctc gttctttata ataggcagtc atttctacta ccatcattga
    11941 agcactcaca gcatctttat cacgtacaaa agtgccatag caaaaaccaa tactttcttc
    12001 atagccaaag agataagttt tatcctttga gatatcatat tcattagctt ttccacaaat
    12061 atttttaaat ccggttaaag tttcaacagt ttcaatatta tatttatctg caataacttt
    12121 tgatagatca ccagttacaa tggattttac caatacagga tgatgtggca aattccctaa
    12181 ggcacatcgt tgtgaaaaaa tataatagga aagaagtgcc cctatcttat taccatttaa
    12241 gaaaatatat tctcctttac tatctttgac ttccaatgct actcggtcac aatctggatc
    12301 tgtggcaagt aagatatctg catcaactga ctttcctaga gattctgaat aggcaaatgc
    12361 tttaggaact tcagggtttg gatagccaac cgttgtaaaa tcaggatcgg gcatttcctg
    12421 ctcaggtacc acataaacat tttcaaaacc acggcgtctt aaaacttcgc gcacaggtaa
    12481 atttcctacg ccatttaaag gagtataaac tactcggact gacttatcaa tgttagtatc
    12541 attaatagtt aaaccaagca cttctttttt atatgcttct tcaatactct catcaatata
    12601 acttgccaaa ccacttgcca gagcctcttc aaagggtatt tgcttaattt gctgataatc
    12661 ggttatagca tccatatgat tggcaatttg gtcagcaata tcatctaaaa tctgagatcc
    12721 ttctttccaa tatgccttat aaccattata agcttgagga ttatgactag cagtaaccat
    12781 cacacccgaa acacatccta gagcacgaat agcatatgag cacattggtg ttgggcgaat
    12841 ccctttataa atataagact taataccatt tgctgccata atggaacaag ttaattctgc
    12901 aaattcctta gattgataac ggacatcata actaactgca attccacgtg caatagcttc
    12961 agggccatga tcaataatcg tattagctaa tgcttgtgct gctttcccca ccatataagt
    13021 attcatacga ttggttcctg ctccaagttt acctctcaat cccgccgttc caaattctaa
    13081 tgttttgtaa aaacgatcct gaatttcaga ttcatcgcct ttaatagctt ctaaatctga
    13141 cttaatatct ttaccgagtg agtcgttttc taaccactct tgataaatct ctttataatt
    13201 catatgagac atcctattct aagaccaaaa tggtaaaaat cagctccatg ataagagatt
    13261 ttagttcttc aggagctaac tgaatagtat caaattaacc ttataaagtc aacttatttt
    13321 ataagttaac tgcacctaat atatttaaga catctaaatt tttttcatca tcctatcact
    13381 cttaatctac attatatata gacttaccaa cttccaaaca atacagtaac aatttactca
    13441 taacaatgga ataacaaaag tctaaaacaa gttgttttag acaatgaaat tataatactt
    13501 tacttaagaa atctcttgtg cggatttctt ttgtctgctc aaatacttcc ttaggggtcc
    13561 cttgctcaac aataattccc gcatccataa aaatgacacg atccgctact tcacgtgcaa
    13621 aacccatttc atgagtgaca ataaccatcg tcataccaga tttagctaaa tcttgcataa
    13681 cagtcaagac ttcacctacc atttcaggat caagagctga agtaggttca tcaaaaagaa
    13741 ggacatcagg attcattgca agacctcttg caatagcaat tcgttgttgt tgtcctccag
    13801 ataagctagc tggataagca ttagccttct ctttgagtcc aactttttca agtagctcgt
    13861 atgcttttgt ctgagcatca agcttagaaa gtccctttgt cttaataggt gataaagtaa
    13921 tattttctag tacagtcata ttgggaaata gattgaactg ttgaaaaacc atgcccattt
    13981 tttcgcgcat tttaaaaata tcattctttt tgtctgttat atcaatccct tcaaaagtca
    14041 ctgttccctt tgttggtact tccaagagat tcattgttct taaaaatgtt gacttaccag
    14101 agccagaagg gccaataata accaccactt ctccttgatg aatatccaag tcaatgcctt
    14161 ttaaaacctc atttttccca aaacttttat gaagcccttg tattgaaata attggctcaa
    14221 ccatctattt ttaccccttt cccaagatat ttctccattt gttttaacaa agctgagaga
    14281 atcgttgtca acattaaata gtaaaatgct gcaaataata acggtgcaac tggtgagtaa
    14341 gtagccgtta caacggattg tgctccgttc cataattcca tgacaccaat agtttgaagg
    14401 agggcactat ccttaataat tgtaataaat tcattcccta gagcaggtaa aatattttta
    14461 aaagcttggg gtaagataac atagcgaagt gtatttttag gtcgaatccc caacgagtaa
    14521 gctgcttcta tttgtccaga tggtacagcc tcaatccctg cacgtacaat ttccgaaata
    14581 taggcaccac tatttaagga aatgataatg ataccaggta aaagtcgtgt aaaatctaaa
    14641 tctaaaacac caaagctaat cgttggtaaa ttgttaaaat gcatccatgc gaaagcaatc
    14701 ataatttgaa ctaccatcgg tgtcccacga aatacccata catagaaatt agctaatatt
    14761 gtgagaaaat gtaaattagt acgctttact aaagcaatta acacgcctat aatagttcca
    14821 aaaaaaacaa cacatgttga aatcataatg gttaccatca caccataatt aaaataggac
    14881 caatattgtg gtaaaaatga aaaattcatt gacatctcct aaaaaagtaa ttgccaatag
    14941 tctatc
//
LOCUS       DBJYSH010000047        14807 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.047, whole genome shotgun sequence.
ACCESSION   DBJYSH010000047 DBJYSH010000000
VERSION     DBJYSH010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 14807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14807
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.047"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            31..1224
                     /locus_tag="ACUBV3_001108"
     CDS             31..1224
                     /locus_tag="ACUBV3_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HHU3847615.1"
                     /translation="MIDFLLLKSLHSLLGLTITVCDQNFSVIREYKSEKTISLFYNHY
                     LILSNFSKTQHDFLFHYGSLGELFLVHHIQQYYIIIGPWRSNVIDPLLLKKKLTETQI
                     NASEQNYFIDRLSQLPFFSLSQIRELLIVTNYCLTGVVKDKLSEPLHYYTKGWSNSFD
                     LDKIKQFSKQNMSSYKYQYHFENNILKAVKSGSEFLLKETVEQFSNSIVPIISGDELR
                     SEKNYSIMIYDRLSQATIQAGLDIETAYRARDRFIKENESTISLNEVLKLRDTAILFY
                     TQQVHSLKKHLETPHSQTIVAVIRYLENNLNRFIKTEEIAKECHMSESKLRKLFKQEK
                     HITIQQYFLNLKIEAAKQLLDENKKVEEVSNLLGFSTSSNFSRTFKKIVGISPLEYKQ
                     KPKTI"
     gene            complement(1419..1943)
                     /locus_tag="ACUBV3_001109"
     CDS             complement(1419..1943)
                     /locus_tag="ACUBV3_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HHU3847616.1"
                     /translation="MVKDRQIQKTKVAIYNAFISLLQENDYSKITVQDVIGLANVGRS
                     TFYSHYESKEVLLKELCEDLFHHLFKQGRDVTFEEYLVHILKHFEQNQDSIATLLLSD
                     DPYFLLRFRSELEHDVYPRLREEYITKVDIPEDFLKQFLLSSFIETLKWWLHQRQKMT
                     VEDLLKYYLTMVER"
     gene            2075..2938
                     /locus_tag="ACUBV3_001110"
     CDS             2075..2938
                     /locus_tag="ACUBV3_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HHU3847617.1"
                     /translation="MTSNKKVAIAFILNISFSVLEFIFGSLFFSGAILADAVHDFGDA
                     IAIGISATLEKKSKKDEDTIFSLGYKRFSLLGALITSLILISGSILVMIENIPKLWHP
                     TPVNYHGMFILAVIAIIINGLASFILHSGQSKHEEILSLHFLEDILGWLAIIVISLIL
                     NWKPLYILDPLLSVAISTFILSKALPKLLSTLKLFLDGVPDSIDYAALHDELKGLSQV
                     RSINQLNIWSMDGIDNRAIIHCCLNQLISEKDCKRAIRTICQHYKINDVTVEIDYSLR
                     EHQNHCKPLKN"
     gene            3023..3865
                     /locus_tag="ACUBV3_001111"
                     /pseudo
     CDS             3023..3865
                     /locus_tag="ACUBV3_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631788.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
     gene            3975..5011
                     /locus_tag="ACUBV3_001112"
                     /pseudo
     CDS             3975..5011
                     /locus_tag="ACUBV3_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861552.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
     gene            5027..5416
                     /gene="nmlR"
                     /locus_tag="ACUBV3_001113"
     CDS             5027..5416
                     /gene="nmlR"
                     /locus_tag="ACUBV3_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stress response transcriptional regulator NmlR"
                     /protein_id="HHU3847618.1"
                     /translation="MHIKEVSKKTGITSDTIRYYERINLIPPIHRSESGIREFTQNDI
                     DILEFVRYFRGAGVSVESLIDYIHLVEKGDSTIPARLEILKDERDNLEDRLQGLQEAL
                     NRLNHKIDNYQNKVVPREQELFDPTTK"
     gene            5426..5821
                     /locus_tag="ACUBV3_001114"
     CDS             5426..5821
                     /locus_tag="ACUBV3_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001158469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HHU3847619.1"
                     /translation="MQETTKSIFPKGEKNPYGEFFIGQSYLAALAKSPDGNVSVGNVT
                     FEAGCRNNWHVHLDGYQILLVTEGSGWYQEEGKEAVSLKPGDVIVTDKGVRHWHGAKK
                     DSEFAHIAITAGKSEFYEAVSDEEYSRLG"
     gene            5845..6162
                     /locus_tag="ACUBV3_001115"
     CDS             5845..6162
                     /locus_tag="ACUBV3_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HHU3847620.1"
                     /translation="MKEKQTAGRRQLEEFAPEFARYNDDILFGEVWAKEDHLTDKTRS
                     IITISALISGGNLEQLEHHLQFAKQNGVTKEEIADIITHLAFYVGWPKAWSAFNKAKE
                     IWI"
     gene            6249..6410
                     /locus_tag="ACUBV3_001116"
     CDS             6249..6410
                     /locus_tag="ACUBV3_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000955819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2316 family protein"
                     /protein_id="HHU3847621.1"
                     /translation="MLTIQEMKDTTFELQENFRRLNYPIKQVAKDLQLNISEVESLLS
                     LDVTYPGDV"
     gene            6410..6532
                     /locus_tag="ACUBV3_001117"
     CDS             6410..6532
                     /locus_tag="ACUBV3_001117"
                     /inference="COORDINATES: protein motif:HMM:NF021585.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2316 family protein"
                     /protein_id="HHU3847622.1"
                     /translation="MLRDYLEDMLKKEGKEVYPFSRLASHSANRWYLYETPWRY"
     gene            6771..7262
                     /locus_tag="ACUBV3_001118"
     CDS             6771..7262
                     /locus_tag="ACUBV3_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HHU3847623.1"
                     /translation="MLSFQIVEKPAMILAGVTLENVKSNQECPKLWEKLHKQYNPSKL
                     KEKGIQQAIGICKTQPDFRFDYSATYRVETSVQAPKGLEIIRIPSATYAVISVKGPMP
                     SSLQETWRKIIQGFFQENNLKPANSPNLEIYSSQHPQDTDYQMEIWLAIADITNKSKT
                     NLI"
     gene            complement(7306..7695)
                     /locus_tag="ACUBV3_001119"
     CDS             complement(7306..7695)
                     /locus_tag="ACUBV3_001119"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000632624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HHU3847624.1"
                     /translation="MITQEMKEIINSQLAMVATVDAKGQPNIGPKRSMRLWDDKTFIY
                     NENTDGQTRINIEDNGKIEIAFVDRERLLGYRFVGTAEIQTEGAYYEAAKKWAEGRMG
                     VPKAVGIIHVERIFNLQSGANAGKEIT"
     gene            complement(7708..10875)
                     /gene="hupY"
                     /locus_tag="ACUBV3_001120"
                     /gene_synonym="lrrG"
     CDS             complement(7708..10875)
                     /gene="hupY"
                     /locus_tag="ACUBV3_001120"
                     /gene_synonym="lrrG"
                     /inference="COORDINATES: protein motif:HMM:NF047961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat adhesin HupY/LrrG"
                     /protein_id="HHU3847625.1"
                     /translation="MTKKHLKTLALALTTVSVVTYSQEVYGLEREESVKQEQTQSASE
                     DDWFEEDNERKTNVSKENSTVDETVSDLFSDGNNNSSSSKTESVVSDHKQVPKAKPGV
                     TQEASNSSNDASKVEVPKQDTASKKETIETSTWEAKDFVTRGDTLVGFSKSGINKLSQ
                     TSHLVLPSHAADGTQLTQVASFAFTPDKKTAIAEYTSRLGENGKPSRLDIDQKEIIDE
                     GEIFNAYQLTKLTIPNGYKSIGQDAFVDNKNIAEVNLPESLETISDYAFAHMSLKQVK
                     LPDNLKVIGELAFFDNQIGGKLYLPRHLIKLAERAFKSNRIQTVEFLGSKLKVIGEAS
                     FQDNNLSNVMLPDGLEKIESEAFTGNPGDEHYNNQVVLRTRTGQNPHQLATENTYVNP
                     DKSLWRATPDMDYTKWLEEDFTYQKNSVTGFSNKGLQKVRRNKNLEIPKQHNGITITE
                     IGDNAFRNVDFQSKTLRKYDLEEVKLPSTIRKIGAFAFQSNNLKSFEASEDLEEIKEG
                     AFMNNRIGTLDLKDKLIKIGDAAFHINHIYAIVLPESVQEIGRSAFRQNGAHHLMFIG
                     NKVKTIGEMAFLSNKLESVNLSEQKQLKTIEVQAFSDNALSEVVLPPDLQTIREEAFK
                     RNHLKEVKGSSTLSQIAFNAFDQNDGDKRFGKKVVVRTHNNSHMLADGERFIIDPDKL
                     SSTMVDLEKVLKIIEGLDYSTLRQTTQTQFREMTTAGKALLSKSNLRQGEKQKFLQEA
                     QFFLGRVDLDKAIAKAEKALVTKKATKNGHLLERSINKAVLAYNNSAIKKANVKRLEK
                     ELDLLTDLVEGKGPLAQATMVQGVYLLKTPLPLPEYYIGLNVYFDKSGKLIYALDMSD
                     TIGEGQKDAYGNPVLNVDEDNEGYHALAVATLADYEGLYIKDILNSSLDKIKAIRQIP
                     LAKYHRLGIFQAIRNAAAEADRLIPKTPKGYLNTVPNYRKKQVEKNSKPVDYKTPIFD
                     KVLPNEKVDGDRVSKGHNIKAETNNSVAVTPTRSEQQLHKSQSDVNLPQTSSKNNFIY
                     EILGYVSLCLLFLVTAGKKGKRARK"
     gene            complement(10938..13106)
                     /gene="nrdE"
                     /locus_tag="ACUBV3_001121"
     CDS             complement(10938..13106)
                     /gene="nrdE"
                     /locus_tag="ACUBV3_001121"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HHU3847626.1"
                     /translation="MTQSDAYLSLNAKTRFRDRTGNYHFASDKEAVEQYMIEHVEPNT
                     MAFTSLIEKLDYLVSNNYYESDLLKQYNLEFICQIFEHAYAKKFTFLNFMGALKFYNA
                     YALKTEDNRYYLEHYEDRVVMNALFLAAGDEKAAYDLVDDMLANRFQPATPTFLNAGK
                     KRRGEYISCYLLRIEDNMESISRAISTSLQLSKRGGGVALCLTNLREFGAPIKGIKNQ
                     ATGIVPVMKLLEDSFSYANQLGQRQGAGAVYLHAHHPEVLTFLDTKRENADEKIRIKS
                     LSLGLVIPDITFELAKANKDMALFSPYDIERVYGKPMSDISITEEYETLLANADIRKT
                     FISARKLFQTIAELHFESGYPYILFEDTVNAKNPHKKEGRIVMSNLCSEIAQVNTASQ
                     FSEDLTFTKVGHDVCCNLGSINIARAMDQAADFEKLIANSIRALDRVSRTSDLDSAPS
                     IKKGNAANHAVGLGAMNLHGFLATNHIYYDSQEAIDFTDCFFYAMAYYAFKASNHLAK
                     EKGTFEGFSESSYADGSYFYQYTEQNFEPKTQRVKNLLAEYGLTLPSQEDWRKLVQSI
                     KEIGLANAHLLAVAPTGSISYLSSCTPSLQPVVSPVEVRKEGALGRVYVPAYKIDADN
                     YVYYKKGAYEVGPEAIINIAAAAQKHVDQAISLTLFMTDQATTRDLNKAYIQAFKQKC
                     ASIYYVRVRQDILEGSESYDDDMLDDFTSSDLEDCQSCMI"
     gene            complement(13108..13521)
                     /gene="nrdI"
                     /locus_tag="ACUBV3_001122"
     CDS             complement(13108..13521)
                     /gene="nrdI"
                     /locus_tag="ACUBV3_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HHU3847627.1"
                     /translation="MDSTVKVVYFSSKSNNTHRFVQKLGCSNQRIPSDGSSILVTEDY
                     ILIVPTYAGGGDDTKGAVPKQVVQFLNVRQNREHCQGVISSGNTNFGDTYAIAGPIIA
                     RKLNVPLLHQFELLGTQEDVTRVKELLCQFTRKDK"
     gene            complement(13522..14532)
                     /gene="nrdF"
                     /locus_tag="ACUBV3_001123"
     CDS             complement(13522..14532)
                     /gene="nrdF"
                     /locus_tag="ACUBV3_001123"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001192438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HHU3847628.1"
                     /translation="MQSYYDRSQSPLDYALSEKAFPMRSVNWNKLNDDKDLEVWNRVT
                     QNFWLPEKIPVSNDLNSWRTLDADWQQLITRTFTGLTLLDSVQATVGDIAQIKHSQTD
                     HEQVIYANFAFMVAIHARSYGTIFSTLCTSQQIEEAHEWVVDTESLQARSRILIPFYT
                     GDDPLKSKVAAAMMPGFLLYGGFYLPFYLSARGKLPNTSDIIRLILRDKVIHNYYSGY
                     KYQQKVAKLSVEKQAEMKTFVFDLLYQLIDLEKAYLYELYDGFDLAEDAIRFSIYNAG
                     KFLQNLGYDSPFTEEETRISPEVFAQLSARADENHDFFSGNGSSYIMGITEETLDEDW
                     EF"
BASE COUNT         4815 a         2683 c         2453 g         4856 t
ORIGIN      
        1 taacatatta aatcaaaggt ggtaactcat atgattgatt ttctactgtt gaaatcatta
       61 cacagtcttc taggtctaac tattactgtt tgtgatcaaa atttttctgt tatcagagag
      121 tataaatccg agaaaactat ttcacttttt tacaatcatt accttatttt aagtaacttt
      181 agtaaaactc aacatgattt tttatttcat tatggctctt taggagaact ttttcttgtc
      241 caccatattc aacaatatta tattatcatc ggtccctggc gtagcaatgt tattgaccct
      301 ttacttctta agaaaaagct gactgaaaca caaatcaatg ctagtgaaca aaactacttt
      361 attgatagac tatcccaatt acctttcttc tcacttagtc aaatccgaga attactaata
      421 gtaaccaatt attgtctaac tggggttgtg aaagataaat tatcagagcc cttacattac
      481 tacacgaaag ggtggagcaa ctcctttgat ctagataaaa ttaagcaatt ttctaaacag
      541 aatatgagtt cttacaaata tcagtatcat tttgaaaata atattctcaa agctgttaaa
      601 tcaggtagtg aatttctttt gaaagaaaca gtggaacaat ttagcaattc tatcgtacct
      661 ataatcagtg gagatgaatt gcgatctgaa aaaaactact ccatcatgat ttatgatcgt
      721 ctctcacagg ctaccatcca agcaggactt gatatcgaaa cagcttatcg agcacgagat
      781 cgttttataa aagaaaacga gtcaactata agtctaaatg aagttttaaa attacgtgat
      841 actgctatct tattctatac tcaacaagtt cattctttaa aaaaacatct cgaaacccct
      901 cattctcaaa ctattgtcgc agtgattcga tatcttgaaa ataatctaaa tcgctttatt
      961 aaaacagaag aaatagctaa agaatgtcac atgagtgaat cgaagttacg aaaactattt
     1021 aagcaagaaa aacacatcac tattcaacaa tattttctaa acttaaaaat cgaagctgct
     1081 aagcaattac tagatgaaaa taagaaagta gaagaagttt ccaatttact tggattttcc
     1141 acttcttcta atttttcaag gacatttaaa aaaatagtgg gaattagccc actagaatat
     1201 aagcaaaagc ctaagacaat atagtaatcg aaaaatattc attttgctta tggtgtcatc
     1261 aacaatccca aagaaactct ttcatcaagg ggactttttt atctcccgaa ttatactcaa
     1321 ttaatctaat aacttcaact cgctatatta gacaatcaat ccattcatca cctctgataa
     1381 cgctaatgaa ataagagaat ctcccaactc tctataattt acctctctac catagttaaa
     1441 taatatttta gtaaatcttc aacagtcatt ttctgtcttt gatgaagcca ccactttaag
     1501 gtctcgataa aactggataa aagaaattgt tttaagaaat cttcaggaat gtccaccttt
     1561 gtaatatact cctcacgcaa tctagggtaa acatcgtgtt ctaattcact tctaaagcgt
     1621 aataaaaaat agggatcgtc agataataat aacgtagcga tactatcttg attttgttca
     1681 aaatgtttta agatatgaac taaatactct tcaaacgtaa catctctacc ctgcttaaat
     1741 aagtggtgaa aaaggtcttc acagagctcc ttgagtaaca cttctttgct ttcatagtga
     1801 gagtaaaaag ttgatcttcc tacatttgca agtccgatta catcttgaac cgttatttta
     1861 gaataatcat tttcctgtag caagcttata aaagcattat aaatcgctac ttttgttttc
     1921 tgaatttgtc tatcttttac catataggac actttctaag ttttgttcag tattgaacaa
     1981 gtgttctttt ttgtttctag caattctctt ttatctccta gataatagaa gaaagaatca
     2041 cactacaata ccagtataac aaaaggagaa aaatatgaca tcaaacaaaa aagtggctat
     2101 cgcttttata ttaaatatca gcttttccgt ccttgaattc atctttggaa gtctattctt
     2161 ctcaggtgct atcttagccg atgctgttca tgattttggt gatgcaatag ctattggcat
     2221 ctcagctact ttagaaaaaa aatctaagaa agatgaagat acgatatttt cattaggtta
     2281 taaacgtttt agccttttag gagctcttat cacaagtcta attcttataa gtggatctat
     2341 cttagtcatg atagaaaata ttccaaaatt gtggcatcct actcctgtta attatcacgg
     2401 catgtttatt ctagctgtca tcgctattat cattaatggt ctagctagtt ttattctcca
     2461 ttctggtcaa agtaaacatg aggaaatatt aagccttcat tttttggaag atattctagg
     2521 gtggttagct attattgtta tatctcttat cttaaattgg aaaccgttgt atatccttga
     2581 tccattatta tcagttgcca tatctacttt catcttatct aaagcacttc ctaaattact
     2641 atcaaccctg aaactttttt tagatggtgt tccagattcc atagattacg ctgcattaca
     2701 tgatgaactt aagggacttt cgcaagtaag atctatcaat caactcaata tctggtcaat
     2761 ggatggtatt gataatcgag caatcattca ctgttgctta aatcaattga tatctgaaaa
     2821 agattgtaaa agagctattc gaacgatttg ccaacattat aagataaacg atgttactgt
     2881 tgaaatcgat tattctttaa gagaacatca aaatcattgc aaaccattaa aaaactaacc
     2941 tgagtgttag ttttttcctt gacttgaagt taactctaag tgctaagatt aggtcagtac
     3001 taatgaaaga aggaaaataa agatgacaga tacaatgata ttaaatgaca atcataaaat
     3061 accaacttta ggtttaggta cgtggatgat agataatgat agggtagctc aagttgttaa
     3121 agatgctatc gaattaggtt accgccacat taatactgca caagcataca acaatgaaaa
     3181 aggtgtcggt gtagggatta aagaatctgg tattacacgt gaatctttat ttgttacgac
     3241 taaaattgcc gctgaacata aagattatga aagtgctaca aaatcaattg atcaatcact
     3301 agaagcttta gggcttgatt gtattgattt aatgattatt catagtccac agccttggaa
     3361 ggaatgacgt gaaacagata aacattttga ccaaggaaat ttagaagctt ggagagcttt
     3421 agaggatgct caaaaatctg gtaaggtaaa atccatcggt gtctcaaact ttttagaaaa
     3481 agacttaaaa aatggtcatg ttaaacctgc tgtcaatcaa atcttagctc acatcggaaa
     3541 tacacctttc gacttgattg attactgtca aagtaaaggt attcaagttg aagcttattc
     3601 accaattgca catggacaag cattgaaatc tgatggtatt caaaaaatgg ctgaaaaata
     3661 tggggttagt gttgctcaat tatgtattca atatttacta caattaaacc tgatcgtttt
     3721 accaaaggct tctagtaaag aacatttaca gtctaatcta gactttgact ttgtaattag
     3781 tgacgaagat atgtcgatat taaaatcact tatgtttgac gattatgggg aattttctaa
     3841 cttccctgtt tttagtgaaa aatgaaacca tatgcggatt tttacagaag tttaattttc
     3901 aaaattttta attttttctt gacttgaagt taactctaag agttataatg aagttataga
     3961 aaagaggtta ggttatgaaa gttgctacat ttattgaacc tggcaaaatg gttataactg
     4021 atacaccaaa accagtcatt gaacaagaga cagatgctgt tatcaaaatt gttagagcct
     4081 gtgtttgtgg ttcagacttg tggtggtatc gtggtatttc aaaacgtgaa agtggctctt
     4141 ttgcaggtca tgaggctatt ggtatcgttg aggaagttgg tactaaagta actgacgtgt
     4201 caaaaggtga ttttgttatt gttcccttta cacatggctg tggccaatgt ccgtcttgta
     4261 aggctggatt tgatggaaat tttacaaatc atcaagctgc aaaaaatgta gataccaagg
     4321 tcaatatcta cgttatacta atgcaaactg ggcattggtt aagatcccag gacaaccttc
     4381 tgattatgat aatgagactc ttaactctct gctcacctta tcagatgtaa tggcaactgg
     4441 ttatcatgct gcagtaactg cagaagttaa agaaggtgat acggtagttg tcatgggaga
     4501 tggtgctgtt ggactctgtg gtgttatcgc tgctaaaatg ttaggtgcta actgtatcat
     4561 tgcaatgagt cgtcacaaag ataggcaaga actagcattg acttttggtg caacggatat
     4621 tgttgaagaa cggggtgacg aagccgttaa acgtgtttta gatttaacca atcaagcagg
     4681 tgccgatgct attttagaat gtgttggtac agagcagtct gttgatacag ctacccaaat
     4741 tgctaggcct ggtgctgtca taggacgtgt tggtatccca caaaatccag acatgaatac
     4801 caataattta ttctggaaaa atatcggcct tagaggtggc attgcttctg taacaacatt
     4861 tgataaatct gtccttttag atgctgttct aactcataaa attaacccag gcttagtttt
     4921 tacaaaatcc tttgtattag atgatattca aaaggcttat gaagcaatgg ataaacgtga
     4981 cgctatcaaa tccttagtga ttgttgactg aatatcgagg ttaaaaatgc atatcaaaga
     5041 agttagtaaa aagactggca ttacgtcaga taccatcagg tattatgaga gaattaatct
     5101 tattccccct attcatcgga gtgaatcagg aattcgtgag ttcacacaaa acgatattga
     5161 tattttagaa tttgtccgct attttagagg cgcaggtgtt tccgttgaaa gtctcattga
     5221 ttatattcat ttggttgaaa aaggcgattc cactatccca gctaggctag aaattttaaa
     5281 agatgaacgc gacaatcttg aagataggct tcaaggcctc caagaagcac ttaatcgtct
     5341 aaatcacaaa atagacaatt atcaaaataa agttgttcct agagaacaag aactttttga
     5401 tccaacaacg aaatgaggaa atcttatgca agaaacaact aagtctatct ttcctaaggg
     5461 tgaaaaaaat ccatatggtg aattctttat tgggcaatcc tatctcgcag cattagcaaa
     5521 atctccagat gggaatgttt cagttggaaa tgtaaccttt gaagcaggct gtcgtaataa
     5581 ttggcatgtt catttagacg gttatcaaat ccttctcgtt acagaaggtt caggttggta
     5641 ccaagaagaa ggaaaagagg cagtgtcatt aaaaccaggc gacgttatcg ttacagataa
     5701 aggtgttaga cattggcatg gtgctaaaaa agattctgaa tttgcacata ttgcgattac
     5761 agctggtaag agtgagtttt atgaagctgt tagtgatgaa gaatattcaa gactaggtta
     5821 aattcaagta aggaggaatc cattatgaaa gaaaaacaaa cagccggacg tcgtcaatta
     5881 gaagagtttg cacctgaatt tgccagatat aatgatgata ttttatttgg tgaggtttgg
     5941 gcaaaagagg atcatttaac agataaaaca cgttctatta ttactatatc tgccttaatt
     6001 agcggaggaa atcttgaaca gttagagcat catcttcaat ttgccaaaca aaacggagtt
     6061 actaaagaag aaattgctga cattatcact caccttgctt tttatgttgg atggccaaaa
     6121 gcttggtcag cctttaataa agctaaggaa atttggatat aaaataacag acatagcaag
     6181 ggagatactt tcccaaaagc caaaaagaaa gatcaaccaa ttattaaacc aactattagg
     6241 agaatgatat gcttactatt caagaaatga aagatacaac ctttgaatta caagaaaatt
     6301 ttagaaggtt aaattatcct attaaacaag ttgctaaaga cttgcaactt aatatcagtg
     6361 aagttgaatc tcttttaagt cttgatgtta cttatcctgg agatgtttga tgctgagaga
     6421 ttatctagaa gatatgttga aaaaagaggg taaagaagtt tatccatttt ctagattagc
     6481 atctcattca gcaaatcgtt ggtaccttta tgaaacacca tggcgttatt aaagactaaa
     6541 gattaatcca tcatctttag agaaataaac aaaaatagtc aatagattct ttttagaaaa
     6601 aagctataaa acaagagcct cttagagaat actctacatc ataagatttc ttctttgaag
     6661 catagcattc caaaagaaaa ttatgattgc taacaagaca attattctaa aaagaaacta
     6721 ttttaacgac tctataatta cttaaggaat cataaagaat aaaggttttt atgctatcat
     6781 ttcaaattgt tgaaaagcca gcaatgatac ttgctggagt aactttagaa aatgtaaagt
     6841 caaatcaaga atgtcctaaa ctttgggaaa aattacataa acaatataat ccatcaaaac
     6901 taaaagaaaa aggtatccag caagccattg gcatttgtaa aacacaacct gattttcgtt
     6961 tcgattacag cgccacttat cgagtagaga caagtgtcca agccccaaaa gggttagaaa
     7021 ttattaggat cccaagcgct acctatgcag tcatttctgt taaaggtcca atgccatcga
     7081 gtcttcaaga aacatggcga aaaataatac aaggattttt tcaagagaat aatctaaaac
     7141 cagctaactc gccaaactta gaaatttatt catctcaaca tccacaagat actgattatc
     7201 aaatggaaat ttggcttgct atcgcagata ttactaataa atcgaaaaca aacttaatct
     7261 agccttgctt aatataaaaa agcccctttt tgaggctttt ttatattaag tgatttcttt
     7321 acctgcatta gcaccagatt gtaaattaaa aatacgttcg acatgaataa taccaactgc
     7381 tttcggaacc cccatgcgtc cttctgccca ttttttagct gcttcgtaat aagcaccttc
     7441 tgtttgaatt tcagcagttc ccacaaacct gtaacctaaa agacgttcac gatcaacaaa
     7501 ggcaatttca attttcccat tatcttcaat gttaatacga gtttgaccat ctgtattttc
     7561 attataaata aacgttttat catcccagag acgcatagaa cgttttgggc caatattggg
     7621 ttgaccttta gcatcaaccg tcgcgaccat tgctaactga ctattaataa tctctttcat
     7681 ttcttgtgta atcatattaa tttctcctta ttttcttgct cgttttcctt tttttccagc
     7741 agttactagg aaaagcaaac ataaactaac gtatcctaga atctcgtata taaaattatt
     7801 tttagaactt gtttgaggta aatttacatc agactgtgac ttatgtaatt gctgctcgga
     7861 ccttgttggt gttacagcta cagaattatt agtctccgcc tttatattat gacctttaga
     7921 cactctatcg ccgtctacct tttcattagg taaaacctta tcaaaaatcg gcgttttata
     7981 atcaactggt tttgaatttt tctccacttg ttttttacga taatttggga ctgtatttag
     8041 gtacccctta ggtgtcttag gaatcaatcg gtctgcttct gccgctgcat ttcggatagc
     8101 ttgaaaaatt cctaatctat gatattttgc caaaggaatc tgacgtattg ctttaatctt
     8161 atcaagggaa ctatttaaaa tatctttaat ataaagacct tcataatcag ctaaagtggc
     8221 aactgccaag gcatgataac cttcattatc ctcgtcaaca tttaatacag gattaccata
     8281 tgcatctttt tgtccctcgc caatagtatc actcatatca agtgcataaa tcaattttcc
     8341 agacttgtca aaataaacgt tcaatccgat ataatattct ggcaatggta aaggcgtctt
     8401 taataaataa actccttgta ccattgtagc ttgcgctaat ggtccttttc cctcgactaa
     8461 gtctgtcagc aagtctaact ctttttccaa gcgcttaaca ttagcttttt taatagcgct
     8521 attattataa gctaataccg ctttgttaat actcctctca agcaaatgac cattctttgt
     8581 tgccttcttg gtcactaaag ccttctcagc tttagctatg gctttatcca aatcaacgcg
     8641 accaaggaaa aattgtgctt cttgaaggaa tttttgtttt tctccttgtc ggaggttaga
     8701 ttttgataac aacgctttac ctgcagtagt catttctcta aactgagttt gagtagtctg
     8761 acgtaatgta gagtaatcta aaccttctat tatttttaaa accttttcaa ggtctaccat
     8821 tgtagaagat agcttatctg gatcaatgat aaaacgctca ccatctgcta acatatgaga
     8881 attattatgt gtcctaacaa ccactttctt accaaagcgt ttgtccccat cattttgatc
     8941 aaaagcatta aaagcgatct gagataatgt agatgaaccc ttcacttctt tcaaatgatt
     9001 tcttttgaaa gcctcttcac gaatagtctg taaatctggc ggtaagacta cttcactaag
     9061 ggcattatcc gaaaaagctt ggacttcaat tgtctttaat tgtttttgct cagagagatt
     9121 tacactttcc agtttattgg ataaaaaagc catttcacca attgttttaa ccttatttcc
     9181 gataaacata aggtgatgtg caccattttg tcgaaaagct gaacgtccta tttcttgtac
     9241 agattctgga agaacaatgg cataaatatg attaatatgg aaagcagcat cacctatttt
     9301 gataagtttg tctttcaagt ctagagttcc aatacgatta ttcataaagg ctccctcttt
     9361 aatctcttct aaatcttcac ttgcttcgaa ggatttcaag ttattagatt gaaaagcaaa
     9421 agcacctatt ttccgaatag ttgaggggag ctttacttct tccaaatcat atttacgtaa
     9481 agttttactt tgaaaatcaa cattgcgaaa agcgttatca ccaatttcag taatagtaat
     9541 accattgtgt tgttttggaa tttctaagtt tttattacgt cttacctttt gtaagccttt
     9601 atttgaaaaa cctgtaacac tatttttttg ataggtaaaa tcttcctcta accatttggt
     9661 ataatccata tcaggcgttg cacgccacaa tgatttgtcc ggattgacgt aagtattctc
     9721 agtcgcaagt tgatgtggat tttggcctgt ccgtgtgcgc aatacgacct gattgttgta
     9781 atgttcatct cctggatttc ctgtaaaagc ttctgattct attttttcaa gtccatccgg
     9841 aagcataaca ttactcagat tattatcttg aaaacttgct tctcctataa ccttaagctt
     9901 acttcccaaa aattcaactg tttgaatacg attagatttg aaagcacgtt ctgctaattt
     9961 tatcaagtga cgtggcaagt aaagcttacc accaatctgg ttatcaaaaa aagctaattc
    10021 tccaatgacc tttaggttat ctggtaattt tacttgtttt aaagacatgt gagcaaaagc
    10081 atagtctgaa atagtctcga gactctcagg aaggttaacc tcagcaatat tcttattgtc
    10141 cacaaaagca tcttgaccaa tagacttata accatttgga atagtaagct tagtcaactg
    10201 gtaagcatta aatatttctc cctcatcaat aatttccttc tgatcaatat ctaaacgact
    10261 cggtttccca ttttctccta gcctacttgt atattctgca atggccgtct ttttatctgg
    10321 agtaaaagca aagctagcta cttgtgtcaa ttgagttcca tctgctgcat gacttggtaa
    10381 aactaagtgt gatgtttgag ataacttatt aattccagat tttgaaaaac ctactaaagt
    10441 atcccctcta gttacgaaat cttttgcctc ccaagttgat gtttctatgg tttccttttt
    10501 tgaagctgta tcctgttttg gtacttctac tttgctagca tcattactag aattgcttgc
    10561 ttcctgtgta acccctggtt ttgctttggg gacttgttta tggtcactta ccactgactc
    10621 ggttttagaa ctagagctat tattatttcc atcagaaaat aaatcactaa ctgtttcatc
    10681 aacagtagaa ttctctttag aaacatttgt tttcctctca ttatcttctt cgaaccaatc
    10741 atcttctgaa gctgactggg tttgttcttg tttgaccgat tcctctcttt ctaatccata
    10801 tacctcctgg ctgtatgtca ctactgatac tgtagtaagt gccaaggcaa gcgttttaag
    10861 atgttttttt gtcattacaa taaagccttt ctaaaagcca atatatttct cttaatcttc
    10921 tgattgagta tatttagtta aatcatgcag gactgacagt cttctaagtc tgatgaagtg
    10981 aaatcatcca gcatatcatc atcataactc tcgctacctt ctaggatgtc ctgtctcact
    11041 cgtacataat aaatagaggc acatttttgt ttaaatgctt gaatataggc tttatttaaa
    11101 tctcgcgtag ttgcttgatc tgtcatgaaa agcgttagcg aaatagcttg atcaacgtgt
    11161 ttttgagcgg cagctgcaat attgataatc gcctcaggtc ccacttcata agctcctttt
    11221 ttgtagtaga cataattatc tgcatcaatc ttataagcag gtacataaac cctccccagt
    11281 gctccttcct tgcgtacttc gacaggtgat acaactggtt ggaggctcgg agtacaagaa
    11341 gaaagataac tgattgaccc tgtcggagca acagctaata aatgcgcatt agctaatcct
    11401 atctctttaa tactttgcac aagctttctc caatcctctt ggcttggtag agtcaggcca
    11461 tactcagcta gtagattttt aacacgctgt gtttttggct caaagttttg ttcggtatat
    11521 tgatagaaat aactgccatc agcataactt gattccgaga agccttcaaa ggttcctttt
    11581 tcttttgcta aatggttaga agctttaaag gcataataag ccatagcgta aaagaagcaa
    11641 tctgtaaaat caatagcttc ttgagaatcg taataaatat ggttagtcgc caaaaatcca
    11701 tgcaaattca tagcacctaa accaacagca tgattagctg cattaccttt tttaatagat
    11761 ggagcactat ctaaatcaga agttcgagat acacgatcaa gggctctaat gctattagca
    11821 attaattttt caaaatccgc cgcttgatcc atagcacgcg caatattaat agaacctaaa
    11881 ttacaacaaa catcatgtcc aaccttagtg aaagtcaggt cttctgaaaa ttgactggct
    11941 gtattaactt gtgcaatctc agaacataaa ttagacataa caattcgtcc ctcttttttg
    12001 tgagggtttt tagcatttac agtatcttca aataagatat aaggataacc agattcaaaa
    12061 tgaagctctg ctatcgtttg aaatagtttt cgtgcactaa taaaggtttt acgaatatcc
    12121 gcattggcca ataaagtttc atattcttct gtgatagaga tatcagacat tggtttacca
    12181 tatactcgct caatgtcata cggtgaaaac aaggccatat ctttatttgc tttagccaat
    12241 tcaaaagtaa tatcaggaat aacaagtcct agggagagag atttaattcg aattttctca
    12301 tcggcattct cacgtttagt atctaaaaat gttaacactt cagggtgatg agcatgtaag
    12361 taaactgcac cagctccttg tctttgtcct aattgattgg cgtaagaaaa cgaatcttct
    12421 agtaatttca tgacaggtac aataccagta gcttgatttt taataccttt tattggagca
    12481 ccaaattccc gtaagttggt taagcaaagt gctaccccac cacctcgttt tgataactga
    12541 aggcttgtac taatagcacg agaaatagat tccatattgt cttcaatgcg caacaaatag
    12601 caagatatat attcaccacg acgttttttc cctgcattta aaaaggtagg tgttgctggt
    12661 tgaaagcgat ttgctagcat gtcatctact agatcatagg cagctttttc atcaccagca
    12721 gctaaaaaca gagcattcat tacaacacga tcttcatagt gttccaaata ataacgattg
    12781 tcttctgttt taagagcata agcattataa aattttaaag cccccataaa atttagaaaa
    12841 gtaaatttct tagcgtatgc atgctcaaaa atttggcaaa taaactcaag gttatattgt
    12901 tttagaaggt ccgattcata gtagttatta gaaaccaaat aatctagctt ttcaattagt
    12961 gatgtgaacg ccatcgtatt aggttcaaca tgttctatca tatattgttc aacagcctct
    13021 ttatccgaag caaaatgata attacctgtg cgatctctaa agcgtgtctt cgcgttcaac
    13081 gagagatatg catctgattg tgtcatctta tttatccttt ctagtgaatt ggcaaagtaa
    13141 ctcctttacc cttgtaacat cttcttgtgt tcccagtaat tcaaattgat gaagcaaagg
    13201 aacatttaat tttcgagcaa taataggtcc agcaatggca taagtgtcac caaaattggt
    13261 atttccagaa gaaataaccc cttggcaatg ttcccgattt tgtcgaacat ttaaaaactg
    13321 tacaacctgt tttggaactg cgcctttggt atcatctcct cctccagcgt aagtaggcac
    13381 aatcaaaata taatcctcag taaccaatat actgctacca tctgatggaa tacgctggtt
    13441 agaacaaccc aacttttgaa caaaacgatg ggtattgtta gatttcgatg aaaaatagac
    13501 caccttcact gtactatcca tttaaaactc ccaatcttca tccaatgttt cttctgtaat
    13561 ccccataata tatgaagagc cgtttcctga gaagaaatca tgattttcat ctgcacgcgc
    13621 cgaaagttgt gcaaaaacct caggagatat acgagtctct tcttctgtaa atggagaatc
    13681 atatcctaaa ttttgtaaaa atttaccagc attgtaaatg ctaaacctga ttgcatcttc
    13741 agccaaatca aaaccatcat atagttcgta aagataggct ttttctaaat caatcaactg
    13801 atagaggagg tcaaagacaa atgtcttcat ttcggcttgc ttttctacag ataatttagc
    13861 aactttctgt tgatacttat agccactgta atagttatga attaccttat ctcgtaaaat
    13921 taaacgaata atatcagacg tatttggtaa ttttcctctg gctgaaaggt aaaaaggaag
    13981 gtaaaagcca ccatataaca aaaatcctgg catcatggct gcagcaactt tagatttcaa
    14041 aggatcgtca ccagtataaa aaggaataag tatacgtgat cgagcttgta gactttcagt
    14101 atccactacc cattcatgtg cttcctcaat ttgttggcta gtgcataagg ttgaaaaaat
    14161 cgttccatac gatcgcgcat gaatagctac cataaaggca aagttagcat aaataacttg
    14221 ttcatgatct gtctgagaat gcttaatctg agcaatatca ccaactgtag cctgcacaga
    14281 atctaatagg gttagaccag taaaagtccg agtaattagt tgctgccaat cagcatccaa
    14341 tgttctccaa gaattaaggt cattagaaac tgggatcttt tcaggtaacc agaaattttg
    14401 agttactctg ttccatacct ctaagtcttt gtcatcgttt agcttattcc aattaacaga
    14461 acgcatagga aaagcttttt ctgacaaggc atagtcaagt ggcgattgag aacgatcata
    14521 gtatgattgc attaaactag tctcctaaat attaaaatta agcgagtttt aagcaccaaa
    14581 aaagcctccc aagaggactg gatttttgtt aaaatcacac aactattgga atgttcttct
    14641 tgatgttttt tcattctggt aagaagctaa ctcttgcttc aaaaccactg ttttgttaag
    14701 tcacattaaa cttttttgtc ggttgccttt attaaagatc tgcttaaaca gtatactaac
    14761 acaaagtttt gtgtctttca acatatatca ccactatatc ttgtgtt
//
LOCUS       DBJYSH010000048        14750 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.048, whole genome shotgun sequence.
ACCESSION   DBJYSH010000048 DBJYSH010000000
VERSION     DBJYSH010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 14750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14750
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.048"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            82..771
                     /locus_tag="ACUBV3_001124"
     CDS             82..771
                     /locus_tag="ACUBV3_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HHU3847629.1"
                     /translation="MGKKILIIEDEKNLARFVSLELLHEGYDVVVETNGREGLDTALE
                     KDFDLILLDLMLPEMDGFEITRRLQAEKTTYIMMMTARDSVMDIVAGLDRGADDYIVK
                     PFAIEELLARVRAIFRRQEIETKTKEKGDSGSFRDLSLNTHNRSAMRGDEEISLTKRE
                     FDLLNVLMTNMNRVMTREELLEHVWKYDVAAETNVVDVYIRYLRGKIDIPGRESYIQT
                     VRGMGYVIREK"
     gene            761..2266
                     /locus_tag="ACUBV3_001125"
     CDS             761..2266
                     /locus_tag="ACUBV3_001125"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HHU3847630.1"
                     /translation="MKNKKDQFIGVKQPLSKKLSQLVFILFFSLFTVFSVLVYTSATR
                     YVLHREKINVGRSLEKTRVRLSQANSSLTSDDILEILYNQVFADDIYPHKRQNGIVRT
                     GESIDSILYVNQEMTLYDVNRKPVFSTLRTGMPTIGKSMGKVIISKVADMEGFVGTKA
                     IYSQKTGQLLGYVQIFYNLGRYYSMRQNIIVFLIMMEVLGTVLALVVINSATKRIVRP
                     VKNLHDLMHQISENPSNLEIRSKVRSEDEIGELSRIFDGMLDQLEDYTRRQSQFISDV
                     SHELRTPVAVVKGHIGLLQRWGKDDPEILEESLAAAYHEADRMSLMINDMLNMIRVQG
                     SLELHQDEVTDLSSSISVVIENFRILREDFQFIFENNISDIVWGKIYKIHFEQALMIL
                     IDNAIKYSPSYKEVSVVLSVDNDFATVVVKDKGEGISDEDIEFIFDRFYRTDKSRNRE
                     STQAGLGIGLSVFKQIMDAYHLKVDIKSELNQGTEFIVRIPIKKFEETKEI"
     gene            2415..2894
                     /gene="nrdR"
                     /locus_tag="ACUBV3_001126"
     CDS             2415..2894
                     /gene="nrdR"
                     /locus_tag="ACUBV3_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HHU3847631.1"
                     /translation="MRCPKCGYNKSSVVDSRQAEEGTTIRRRRECEKCGNRFTTFERL
                     EELPLLVIKKDGTREQFSRDKILNGIIQSAQKRPVSSEDIENCILRIERKIRSEYEDE
                     VSSITIGNLVMDELAELDEITYVRFASVYKSFKDVDEIEELLQQITKRVRSKKSGSV"
     gene            2894..4069
                     /locus_tag="ACUBV3_001127"
     CDS             2894..4069
                     /locus_tag="ACUBV3_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HHU3847632.1"
                     /translation="MSPIDEFTYIKQNKIVYDSNSLIQLYFPIMGSDAMALYDYFVHF
                     FDDGIRRHKFSEVLNHLQYGMPRFQDALVMLTALDLLTVYQATGTYLVKLNQAMSNEL
                     FLSNPIYRRLLEKRIGEVAVAELDMKIPKNARDISKKFTDVFSDLGQPKQEVNRSKNV
                     FDLESFKRLMMRDGLRFNNEKDDVLGIYSVSELYHLNWYDTYQLAKQTAINGMIAPQR
                     MKVQQNEGQHIKDNQSFTNNEKVILRESKNDSALVFLEKIKRSRKSVTTSGEKTLLED
                     LAKMNFLDEVINVMVLYTLNKTKSANLNKAYIMKVANDFAFQNVMTAEDAVLKIRDFS
                     DQKVRTKTETKKKQSNVPEWSNPDYKDEVSPEKEIELEQFKTDALKRLERLGKDGES"
     gene            4066..4968
                     /gene="dnaI"
                     /locus_tag="ACUBV3_001128"
     CDS             4066..4968
                     /gene="dnaI"
                     /locus_tag="ACUBV3_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HHU3847633.1"
                     /translation="MKSVGQALENQGRVPRNTNDELIQMILSDAQVAEFIKTHQLSQR
                     EINISMSKFNQFLIERQKFKNKDSQYIAKGYEPILVMNEGYADVSYLETRELIEAQKK
                     QAISDRINLVNLPKSYRNIRMTDFDINNESRMKAMSQLLDFVETYPSYNHKGLYLYGD
                     MGVGKSYLMAAMARELSERKGVSTTLLHFPSFAIDVKNAISSGTVKDEIDAVKSVPIL
                     ILDDIGAEQATSWVRDEILQVILQHRMLEELPTFFTSNYSFNDLERKWANIKGSDETW
                     QAKRVMERVRYLAIEFHLEGPNRR"
     gene            5016..6326
                     /gene="der"
                     /locus_tag="ACUBV3_001129"
     CDS             5016..6326
                     /gene="der"
                     /locus_tag="ACUBV3_001129"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HHU3847634.1"
                     /translation="MVLPTVAIVGRPNVGKSTLFNRIAGERISIVEDVEGVTRDRIYT
                     TGEWLNRKFSLIDTGGIDDVDAPFMEQIKHQADIAMTEADVIVFVVSGKEGVTDADEY
                     VSRILYKTNKPVILAVNKVDNPEMRNDIYDFYSLGLGDPYPLSSVHGIGTGDILDAIV
                     ENLPVEEENENPDIIRFSLIGRPNVGKSSLINAILGEDRVIASPVAGTTRDAIDTNFV
                     DSQGQEYTMIDTAGMRKSGKVYENTEKYSVMRSMRAIDRSDVVLMVINAEEGIREYDK
                     RIAGFAHEAGKGIIIVVNKWDTIEKDNHTVSQWEADIRDNFQFLSYAPIIFVSAETKQ
                     RLHKLPDMIKRISESQNKRIPSAVLNDVIMDAIAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYLRFLENQIREAFVFEGTPINLIARKRK"
     gene            6482..9580
                     /locus_tag="ACUBV3_001130"
     CDS             6482..9580
                     /locus_tag="ACUBV3_001130"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000089139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HHU3847635.1"
                     /translation="MSRMIPGRIRNQGIELYEQGLVSLISQEGNLLKAKVGDCQIEYS
                     LVTEETKCSCDFFARKGYCQHLAALEHFLKNDPEGKAILSKVQVQQESQQETKKKTSF
                     GSVFLDSLIINEDDTIKYQLSAQGEQNPYANDIWWTLKIRRLPDDRSYVIRDIKAFLN
                     TVRKEAYYQIGKQYFETLSLIQFDETSQELIEFLWRLIPSHSSKIDLEFILPNQGRHL
                     SLTRGFFEEGVTLMNALENFSFESDFHQFNHLYFKELEGEDHLYQFKVIVHRQSIELE
                     IKEKDLKPLFANSYLFYRDTFYHLNLKQEKMVTAIRSLPIEGDLAKHIHFDLDDQDKL
                     AAHLLDFKEIGLVDAPRSFSIHDFKVNFEFDINSQNEILLQMVFDYGNDLTVHNRQEL
                     EQLTFASHFKHEEKIFKLLERYGFAPHFSTSHPAYSAQELYDFYTYMLPQFKKMGTVS
                     LSAKLESYRLIERPQIDIEAKGSLLDISFDFSDLLENDVDQALVALFDNNPYFVNKSG
                     QLVIFDEETKKVSATLQGLRARRAKNGHIELDNIAAFQLSELFANQDNVSFSQHFYQL
                     IEDLRHPEKFKIPGLSVSASLRDYQLTGVRWLSMLDHYGFAGILADDMGLGKTLQTIS
                     FLSTKLTRDSRVLILSPSSLIYNWQDEFHKFAPDVDVAVAYGSKIRRDEIIAERHQVI
                     ITSYSSFRQDFETYSEGNYDYLILDEAQVMKNAQTKIAHSLRSFEVKNCFALSGTPIE
                     NKLLEIWSIFQIILPGLLPGKKEFLKLNPKQVARYIKPFVMRRRKEEVLPELPDLIEM
                     NYPNEMTDSQKVIYLAQLRQIQESIQHSSDADLNRRKIEILSGITRLRQICDTPRLFM
                     DYDGESGKLESLHQLLTQIKENGHRALIFSQFRGMLDIAEREMVAMGLTTYKITGSTP
                     ANERHEMTRAFNAGSKDAFLISLKAGGVGLNLTGADTVVLIDLWWNPAVEMQAISRAH
                     RLGQKENVEVYRLITRGTIEEKILEMQETKKHLVTTVLDGNETHASMSVDDIREILGV
                     SK"
     gene            9745..10362
                     /locus_tag="ACUBV3_001131"
     CDS             9745..10362
                     /locus_tag="ACUBV3_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3847636.1"
                     /translation="MNRGSRRFPLIADDEPVMSPLVKMNLYDNEDLINNIRGFYQEKT
                     YQSMVKSNYEHEEISHPKVIENDPVPPQSFVKKATELSKSRQEAKRSVREKRQAYYAK
                     QEFKAPSKEAFQQQLKATVPKKQTQRKVTELSHLSDRLQQESYILAEIPIIFQEPDNT
                     PNPKTKKNNFDFLKRSQVYNKQDNQFHKERAKAQELNLTRFKDIN"
     gene            10372..11703
                     /gene="murC"
                     /locus_tag="ACUBV3_001132"
     CDS             10372..11703
                     /gene="murC"
                     /locus_tag="ACUBV3_001132"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HHU3847637.1"
                     /translation="MSKTYHFIGIKGSGMSALALMLHQMGHNVQGSDVDKYYFTQRGL
                     EQAGVTILPFSPNNISEDLEIIAGNAFRPDNNEELAYVIEKGYHFKRYHEFLGDFMRQ
                     FTSLGVAGAHGKTSTTGLLAHVLKNITDTSFLIGDGTGRGSANANYFVFEADEYERHF
                     MPYHPEYSIITNIDFDHPDYFTGLEDVFNAFNDYAKQVQKGLFIYGEDSKLHEITSKA
                     PIYYYGFEDSNDFIAKDITRTVNGSDFKVFYNQEEIGQFHVPAYGKHNILNATAVIAN
                     LYIMGIDMALVAEHLKTFSGVKRRFTEKIIDDTVIIDDFAHHPTEIIATLDAARQKYP
                     SKEIVAIFQPHTFTRTIALLDDFAHALSQADSVYLAQIYGSAREVDNGEVKVEDLAAK
                     IVKHSDLVTVENVSPLLNHDNAVYVFMGAGDIQLYERSFEELLANLTKNTQ"
     gene            11789..12292
                     /locus_tag="ACUBV3_001133"
     CDS             11789..12292
                     /locus_tag="ACUBV3_001133"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000586872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HHU3847638.1"
                     /translation="MIIRNGCLEDLQQVISIERINFSEAEAASKKAMQERLMIMTDTF
                     LVAEINGRLAGYIEGPVIKGRYLTDDLFHKVSEIPVREGGFIGITSLSIHPDFKGQGI
                     GTALLAAMKDLVVSQERDGISLTCHDDLISFYEMNGFKDEGESDSQHGGSLWYNMIWN
                     NPTRSIC"
     gene            12379..14181
                     /gene="mltG"
                     /locus_tag="ACUBV3_001134"
     CDS             12379..14181
                     /gene="mltG"
                     /locus_tag="ACUBV3_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HHU3847639.1"
                     /translation="MTEFNDDQHSNHDQKSFKEQILAELEEANRLRKLREEELYQKEQ
                     EAKEAARRTAQLMADYEAQRLKDEQEARAKALETKQRLEEQEKARIEAKLLAEAAREE
                     ERRQAEQALASQEEQVINQGMEPSRELDSGSKSSEFRTTENVPDIDLKADKTDVATAV
                     PNQETEEIFLVRATDIPTEGENVKLGETSELEPVAKEPIRVEDLSKEEEDIALSAKNK
                     HNKRERRQKADNVAKRIARILISIIILVLLLTAFVGYRFVDSAIKPVDSNSNKFVQVE
                     IPIGSGNKLIGQILEKAGVIKSATVFNYYSKFKNYSNFQSGYYNLKKSMTLDQIAAEL
                     EKGGTAEPTKPALGKILITEGYTIKQIAKAIESNKIDTKTTSTPYKADDFLKLVQDET
                     FIKKMVAKYPNLLGSLPDKSKAIYQLEGYLFPATYNYYKDTTLEGLVEDMISTMNTKM
                     APYYNTIKAKNMSVNDVLTLSSLVEKEGSTDEDRRKIASVFYNRLSAGQALQSNIAIL
                     YAMGKLGDKTSLAEDAQINTSIKSPYNIYTNTGLMPGPVDSPSISAIEATIKPASTDY
                     LYFVADVKTGNVYYAKDFETHKANVEKYINSQIN"
     gene            14254..14736
                     /gene="greA"
                     /locus_tag="ACUBV3_001135"
     CDS             14254..14736
                     /gene="greA"
                     /locus_tag="ACUBV3_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HHU3847640.1"
                     /translation="MAEKTYPMTQVEKDQLEKELEELKLVRRPEVVERIKIARSYGDL
                     SENSEYDAAKDEQAFVEGQIQILETKIRYAEIIDSDAVAKDEVAIGKTVLVQEVGTND
                     KDTYHIVGAAGADIFSGKISNESPIAHALIGKKTGDLATIESPAGSYQVEIISVEKTN
                     "
BASE COUNT         5060 a         2168 c         2999 g         4523 t
ORIGIN      
        1 atgtgggcta tcttgttttt tatctagata ttctaaaaaa taattgatag tgttaagtaa
       61 agaataagaa ggttggtgta gatgggtaaa aagatcttaa taatcgaaga tgagaaaaat
      121 ttagctcgct tcgtctcgtt agaactacta catgaaggat atgatgttgt tgttgaaaca
      181 aacggtcgtg aaggattgga cacagcatta gaaaaagatt ttgatttgat tctactggat
      241 ttaatgcttc cagagatgga tggtttcgaa atcacacgtc gcctgcaggc tgaaaaaaca
      301 acctatatca tgatgatgac agcacgtgat tctgttatgg atattgtagc tggtcttgat
      361 cgtggagcag atgattatat tgttaagccg tttgcaatcg aagaattatt agcacgtgtt
      421 agagcgattt tccgacgcca agaaattgaa acgaagacaa aagaaaaagg tgatagtggt
      481 agtttccgtg atttgtcatt aaatactcat aatcgttcag cgatgcgtgg tgatgaggaa
      541 atttcactaa caaaacgtga atttgatttg ttgaatgtct tgatgacaaa tatgaatcgt
      601 gttatgacac gagaagagtt gctagaacat gtttggaaat acgatgtggc agcagagaca
      661 aacgttgttg atgtttatat ccgttaccta agaggtaaaa ttgatatccc aggtcgtgaa
      721 tcatatattc aaactgttcg cggaatgggc tatgtgattc gtgaaaaata aaaaagatca
      781 gtttataggg gtaaaacaac ctctgtcaaa aaaattatcg caattagttt ttatcttatt
      841 ttttagccta tttacagtat tttctgtttt agtttatact tcggcaacaa gatacgtcct
      901 tcacagggaa aaaattaatg tcggtaggtc attagaaaaa actagagttc gtttgtcgca
      961 agcgaactct agtttgacat ccgatgatat tttagagatc ctgtacaacc aggtttttgc
     1021 ggatgacatc tatcctcata agcgtcaaaa tggaattgtt agaacgggcg agtcaatcga
     1081 tagtattctt tatgttaatc aagaaatgac cctatacgat gtcaacagga aaccagtttt
     1141 ttctacactg agaacgggta tgccaactat tggtaaatcc atgggaaaag ttatcatctc
     1201 taaagttgca gatatggaag gttttgttgg tacaaaagca atctactctc aaaaaacagg
     1261 acagttgctt ggatacgttc aaattttcta taatttaggt cgttattata gtatgaggca
     1321 aaacatcatt gttttcttga ttatgatgga agttttaggg actgttttag ccttggtcgt
     1381 tattaatagt gctactaagc gtattgttcg tccggttaaa aatttacacg acttaatgca
     1441 tcagatatct gaaaatccta gtaacttaga aattagaagt aaggtccgtt cagaagacga
     1501 aattggagaa ttgtctagga tttttgatgg gatgttggat cagttggaag actatactcg
     1561 tcgacaatca caattcatta gtgatgttag tcatgaatta agaacgcctg tcgctgttgt
     1621 aaagggtcat attggtttat tacaacgttg ggggaaagat gatccagaaa ttcttgaaga
     1681 aagtttagca gcggcatacc atgaggcaga tcgtatgtcc ttaatgatta atgacatgtt
     1741 aaatatgatc cgtgtccaag gaagcttgga acttcatcaa gatgaagtga cagatttatc
     1801 aagctctata tcagttgtta ttgaaaattt ccgtattttg agagaagatt ttcaatttat
     1861 ttttgaaaat aatatttcag atatcgtttg gggaaagata tataagatac attttgagca
     1921 agctctcatg atcttaattg ataacgctat caagtattct cctagttata aagaagtcag
     1981 tgttgtttta tcagtagaca atgattttgc gacagtagtt gtaaaagata aaggagaagg
     2041 tatctcagat gaagatattg agtttatctt tgatcgtttt tatcgaactg ataaatcacg
     2101 taacagagaa agtacacaag ctggtttagg tatcgggctc tcagtgttta aacaaattat
     2161 ggatgcttac catttaaagg tggatattaa aagtgaacta aatcaaggga ctgaattcat
     2221 tgttcgcatt cctataaaaa aatttgaaga aactaaagaa atataagaaa aatccttatg
     2281 cttaatgata aaaggcataa ggattatttt gtgttttgat atgatgaggt gataagagag
     2341 gcgcttcttt tgttattact gaatttgtgt taaaataaga tgattaaatg atatgataga
     2401 ataggggtgt tgacatgcgt tgtccaaaat gtggctataa taaatctagt gtggttgata
     2461 gccgacaagc tgaagaaggt acgactatta gacgccggag agagtgcgaa aaatgtggca
     2521 accgctttac aacatttgaa cgtttggaag aattgcctct tttagtcata aagaaagatg
     2581 gtactcgcga gcaattttct cgagataaaa ttttgaatgg tattattcag agtgctcaaa
     2641 aacgccctgt ttcgagcgaa gatattgaaa attgtatttt acgtatcgaa cgtaaaatta
     2701 ggagtgagta tgaagatgag gtttctagta tcactatcgg aaatttagtg atggatgaac
     2761 tcgccgaatt agatgagatt acttatgtgc gctttgctag tgtttataaa tcttttaaag
     2821 atgtagacga aattgaagaa ctacttcaac aaattacaaa gcgcgtacgg agtaaaaaga
     2881 gtggtagcgt gtgatgagtc caatagatga gtttacatat attaagcaaa ataagattgt
     2941 ttatgatagt aatagtttaa tacaattgta tttcccaatt atgggaagtg atgccatggc
     3001 actctatgat tattttgttc atttttttga tgacggaata cgacgtcata aattttcaga
     3061 ggttctcaat catctgcaat acgggatgcc aagatttcaa gatgcattgg taatgttaac
     3121 agctttggat ttgttgactg tttaccaagc cacaggtact taccttgtta agttgaatca
     3181 agctatgtca aatgagttat ttttatctaa tcccatctat cgtcgtttgt tagaaaaacg
     3241 tataggagag gtagcagtgg cagaattaga tatgaaaata cctaaaaatg ctagagatat
     3301 ctctaaaaaa tttacagatg ttttctcgga tttaggacaa ccaaaacaag aggtaaaccg
     3361 gagtaaaaat gtttttgact tggaaagttt caaacgtttg atgatgcgag atggcttacg
     3421 ctttaacaat gaaaaagatg atgtcttagg tatttatagt gtatcagaac tttatcattt
     3481 aaattggtat gatacctatc aattagcaaa acaaacagct atcaatggaa tgattgcgcc
     3541 tcagcgaatg aaagttcaac aaaatgaggg gcagcatatt aaagataatc agagttttac
     3601 aaataatgaa aaggtcatac taagggaatc taaaaatgat agtgctctag tgttccttga
     3661 aaaaatcaag agaagccgta agtcagtgac aacaagtggt gagaagactt tacttgagga
     3721 tttggcaaaa atgaatttcc tagacgaagt cattaatgtt atggttttat ataccttgaa
     3781 taagacaaaa tctgctaact taaataaggc ctatatcatg aaagttgcta atgattttgc
     3841 ctttcagaat gttatgacgg ccgaagatgc tgtgcttaaa attcgtgatt tttcagatca
     3901 aaaagtaagg actaaaacag aaacgaagaa gaaacaatcg aatgttcctg aatggagtaa
     3961 tcctgattat aaagatgagg ttagcccaga aaaagaaatt gaattagaac agtttaaaac
     4021 ggatgccctc aagcgtttag aacgattagg aaaggatggt gaatcatgaa atcagtggga
     4081 caagcattag aaaatcaagg gagagtgcct cgcaatacaa atgatgagct tattcaaatg
     4141 attttatcag atgctcaggt agcggagttc attaaaaccc accaactttc tcagcgtgaa
     4201 ataaatatca gcatgtctaa atttaatcaa tttttgattg agcgtcagaa atttaaaaat
     4261 aaggatagtc aatatattgc taaaggctat gagcctattt tggttatgaa tgaaggttat
     4321 gcagatgttt cttatttaga aacacgagag ttaattgaag cacaaaaaaa acaagctatc
     4381 agtgatcgta ttaatttagt taatctaccc aagtcctatc ggaatatcag aatgactgat
     4441 tttgatatta ataacgagag tcgaatgaaa gctatgtcac aactgcttga ttttgtagag
     4501 acttatcctt catataatca taaggggctt tatttatatg gagatatggg ggttgggaaa
     4561 tcctatttaa tggcagcaat ggcgagggaa ttatcagaga gaaagggtgt gtcaacgact
     4621 cttcttcatt tccctagttt tgctattgat gtcaaaaatg ctatttcaag cggaactgta
     4681 aaagatgaaa ttgatgctgt gaaaagtgtt ccaattttga ttttagatga tattggggct
     4741 gaacaggcaa cgtcttgggt tcgtgacgag attttacaag ttatattaca acatcgtatg
     4801 ctagaagagt tgcccacttt ctttacgtca aattatagtt ttaacgattt ggaaagaaaa
     4861 tgggctaata taaaaggaag cgacgaaact tggcaagcga aacgagttat ggagcgcgta
     4921 cggtatttag cgatagaatt ccacttagaa ggaccgaatc gccgttaata aaatagaatg
     4981 gaattgggag tattcccaat agtgagagga gaattatggt attacctaca gttgctattg
     5041 ttggtcgtcc gaatgttgga aaatcaacat tatttaacag aattgcagga gaacgtattt
     5101 ccattgttga ggacgttgaa ggagtcactc gtgatcgtat ctatacaacg ggtgagtggc
     5161 taaatcgtaa gttttcatta attgatactg gtgggattga tgatgttgat gcaccgttta
     5221 tggagcaaat taagcaccaa gcagacattg ctatgacaga ggctgatgtt attgtgtttg
     5281 ttgtgtcggg taaagaaggt gtgactgatg cagatgagta tgtctcacgt attctttata
     5341 agaccaataa accggttatt ttagctgtta ataaggtaga taatcctgag atgcgtaatg
     5401 atatttacga tttctactct cttggtttag gggatcctta tcccttgtca tcagttcatg
     5461 gtattggtac tggtgatata ttggatgcta ttgtggaaaa tcttccggta gaagaggaaa
     5521 atgaaaatcc agatattatt cgttttagtt tgattggacg tccaaatgtt gggaaatcta
     5581 gtttaattaa tgccatcctt ggggaagacc gtgttattgc tagcccagtt gctggtacta
     5641 cgcgcgatgc tattgatact aatttcgttg atagccaggg acaagagtat actatgattg
     5701 atactgctgg tatgcgtaaa tctggtaagg tatatgaaaa cacagagaaa tattctgtta
     5761 tgcgttctat gcgcgccatc gaccgctcag atgttgttct tatggtgatt aatgcagagg
     5821 aaggtattcg tgagtatgac aagcgtattg cagggtttgc acatgaagct ggtaaaggta
     5881 ttattattgt agttaataaa tgggatacta tcgaaaaaga caatcatact gtcagtcagt
     5941 gggaagctga tatacgagat aacttccaat tcttgtctta tgcgccaatt atctttgttt
     6001 ctgcagagac aaaacaacgt ttgcacaagc taccagatat gattaaacgt attagcgaaa
     6061 gtcaaaacaa acgtattccg tctgctgtat taaatgatgt gattatggat gctattgcta
     6121 ttaatccaac accaactgat aaaggaaaac gtcttaagat tttctacgca actcaagttg
     6181 ctgttaaacc accaactttc gttgtttttg ttaacgaaga agaattgatg cacttttcat
     6241 atcttcgttt cttagaaaat caaatccgtg aagcatttgt ttttgaagga acaccaatta
     6301 atctaatagc acgtaaacgt aaataacatc ttattgttga aattgaaata gaaggttagg
     6361 acagttgttt tagccttttt ttatttgtta ctgtatcaaa aaacaatcaa tttagtattt
     6421 ttacaagaaa ttggacttga attttgttat aattgaggga taagatgaag gtggtagcat
     6481 aatgagtaga atgattccag gacgtattcg aaatcaaggt atagaactct acgaacaagg
     6541 tttggtgtcc ttgatttctc aagaaggtaa tctattaaaa gctaaagttg gggattgtca
     6601 gattgaatat tcattagtaa cagaagaaac aaaatgttct tgtgattttt ttgcaagaaa
     6661 aggctattgt caacatttag ctgctctgga gcatttcttg aaaaacgacc cagagggtaa
     6721 agcaatctta tctaaagttc aagttcaaca agaaagtcaa caagaaacta agaagaaaac
     6781 ctcttttggt agtgtttttt tagatagtct aattattaat gaagatgata ctataaaata
     6841 tcaactatcg gctcaaggag aacaaaatcc ttatgctaat gatatctggt ggactttaaa
     6901 aattagacga ctgcctgatg atagaagtta tgttattcgt gacatcaaag cttttttaaa
     6961 tactgttcga aaagaagctt attatcagat tggaaaacaa tactttgaaa ctttgtcttt
     7021 gatacagttt gatgagacca gtcaagaatt gatagaattt ttatggaggc tcattccaag
     7081 tcattcaagc aaaattgatc tagagtttat tttaccaaat caaggtcgtc accttagtct
     7141 cactcgtggt ttttttgaag agggcgttac tttgatgaat gctttagaaa atttttcatt
     7201 tgaaagtgat tttcatcaat ttaatcacct ctactttaag gaattagaag gtgaggatca
     7261 tctttatcaa tttaaggtta ttgtccatag gcaatcaatt gaattagaaa tcaaagagaa
     7321 agacttaaaa ccactatttg cgaattctta tttgttttat cgcgatactt tctaccatct
     7381 taatttgaag caagaaaaga tggtaacggc tattcgtagt cttcccattg agggagattt
     7441 agcaaaacat atccattttg atttagatga tcaagataag ttagcagctc atttactgga
     7501 ttttaaggag attggactag tagatgctcc acggagcttc tctattcatg attttaaggt
     7561 aaattttgag ttcgatatta atagtcaaaa tgaaatttta ttgcaaatgg tatttgatta
     7621 tggtaatgat ttaactgttc ataatcgtca agaactagaa cagttaacat ttgctagtca
     7681 cttcaaacat gaagaaaaaa tatttaaact cttagaaaga tatggctttg caccccactt
     7741 ttcaaccagc catccagcat acagtgcaca agaattatac gatttttata cctatatgct
     7801 tccacaattt aaaaaaatgg gaacagtgag cttatccgca aaattggaaa gttatcgtct
     7861 catagaacga ccgcaaattg atattgaagc taagggaagc ctgctagata tttcttttga
     7921 tttctctgat ttactggaaa acgatgttga tcaagctctg gtagctttgt ttgacaataa
     7981 tccttatttt gtgaataaat cagggcaatt agttattttt gatgaagaga ctaagaaagt
     8041 aagtgcaact ttgcaagggt tgagagctag acgagcaaag aatgggcata ttgaattaga
     8101 taatattgca gcatttcaat tatcagaact gtttgcaaat caagataatg ttagcttttc
     8161 acaacatttt taccaattga ttgaagattt gagacaccca gaaaagttta aaatacctgg
     8221 tctatctgtt tcagcttcct tgcgcgatta tcagcttaca ggtgtccgtt ggttaagtat
     8281 gttagatcat tatggctttg cagggatttt agctgatgat atggggcttg gaaaaacact
     8341 tcaaacgatt tcatttttaa gtacaaaact aactagagat tctcgtgtgc ttattttatc
     8401 accatcaagt ttaatttata attggcaaga tgaattccac aaatttgcgc cagacgtaga
     8461 tgtagcagtt gcctatggtt ctaagattcg tagagatgaa attattgctg aacgtcatca
     8521 agtaatcatt acaagttatt catcatttag gcaggatttt gagacttatt ctgaaggaaa
     8581 ttatgattat ttaattttgg atgaagcaca ggttatgaaa aatgctcaaa ctaaaatagc
     8641 ccatagcttg agatcttttg aggtcaaaaa ctgttttgca ttatcgggaa cacctattga
     8701 gaataaactg ttagaaattt ggtccatttt ccagatcatt ttacctggtt tattacctgg
     8761 taagaaagag tttttgaagt tgaaccctaa acaagttgct cgttatatca aaccatttgt
     8821 gatgagacgt cgaaaagaag aagttctccc agaattaccg gatttgattg aaatgaacta
     8881 tccaaatgaa atgacagaca gtcaaaaagt tatctatttg gcgcaattac gccaaattca
     8941 agaaagtatc caacattcta gtgacgctga cttaaatcgt cgtaaaattg aaattttatc
     9001 tggcatcaca cgattacgtc aaatttgcga cactccacgt ctttttatgg attatgatgg
     9061 tgaaagcggt aagttagaaa gcctacatca acttttaaca caaattaagg aaaatggaca
     9121 tcgtgctctg attttttctc agttcagggg tatgttagat atcgcagaac gtgagatggt
     9181 agctatgggg cttacaactt ataaaattac tggttctaca ccagcaaatg aacgtcatga
     9241 aatgaccaga gcatttaatg caggatcaaa agatgctttc ttgatttctt taaaagctgg
     9301 tggcgttggt ctcaacctta caggagctga taccgttgtt ctgattgact tgtggtggaa
     9361 tccagctgtt gaaatgcagg cgattagtcg cgcacatagg ttggggcaaa aagagaatgt
     9421 cgaggtttat cgcttgatta ctagaggcac aatcgaagaa aaaattctag aaatgcaaga
     9481 aactaaaaaa catttggtta caacagttct ggatggaaat gaaacccatg ctagcatgag
     9541 tgtagatgat attcgagaga tacttggagt ttctaaataa atatgataat gtgactataa
     9601 tataaaaaag aaagtggtct ctcgctttct tttttatata aatgaatgaa aaataccaat
     9661 ctgataagct ttacttagtt gaaaactttg aaaaaacaga aaaattagtt tataataggt
     9721 ctgttgtaga aaggaagtga taatgtgaat agaggctcta gacgatttcc gttgatagca
     9781 gatgatgaac cagtaatgtc tccgcttgta aagatgaatc tttatgataa tgaagattta
     9841 attaacaaca ttcgaggctt ttatcaagaa aaaacatatc aaagcatggt caaaagcaat
     9901 tatgaacatg aagaaatatc acatcctaag gtaattgaaa atgatcctgt accaccgcag
     9961 agttttgtaa aaaaagcgac agagttgagc aaatctcgac aagaggcaaa gaggtctgtt
    10021 cgggagaaac gacaagctta ctatgcgaaa caggaattta aggcgccatc aaaggaagct
    10081 tttcagcaac aattgaaggc aacagtaccg aaaaaacaaa ctcagcgaaa ggtgactgaa
    10141 ctaagtcatc tttctgatcg tttacagcag gaaagctata tattagcaga aattccaatc
    10201 atatttcaag aacctgataa tacaccgaac ccaaaaacaa agaaaaataa ttttgatttt
    10261 ttgaaacgca gtcaagttta caataaacaa gacaatcagt ttcacaaaga aagagccaag
    10321 gctcaagaat taaatctgac acgttttaaa gacattaatt aaggagagac catgtcaaaa
    10381 acttatcatt ttattggtat taaaggatcc ggaatgagtg ccctagcact gatgcttcat
    10441 caaatgggac ataacgtcca aggaagtgac gttgacaaat attattttac ccaacgtggt
    10501 ttagagcaag caggtgtaac tatattacct ttctcaccga ataatatcag tgaggattta
    10561 gagattattg caggaaatgc ttttcgtcca gataacaatg aagagttggc ttatgttatt
    10621 gaaaagggct atcattttaa acgatatcat gaatttctcg gagattttat gcgtcagttc
    10681 actagtctag gtgtagctgg ggcacatgga aaaacctcaa cgacaggttt attagctcat
    10741 gttttaaaaa atattacaga cacttctttc ctaattggag atggtacagg acgtggttct
    10801 gctaatgcta attactttgt gtttgaagct gatgaatacg aacgtcattt tatgccgtac
    10861 catccagaat actcaattat taccaatatt gattttgacc atcctgatta ttttacaggc
    10921 ctagaggacg tattcaatgc ttttaatgac tatgctaagc aagttcaaaa aggtttattc
    10981 atttatggag aagattcaaa acttcatgaa atcacttcta aggcaccaat atattattat
    11041 ggttttgaag attcaaatga ttttatagca aaagacatca ctcgaactgt taatggttct
    11101 gactttaagg ttttctataa ccaagaagaa attggtcagt ttcatgtacc agcatacggt
    11161 aaacataata tcttaaatgc aactgctgtt attgctaacc tttacataat gggaattgat
    11221 atggcattag tagctgagca tttgaagaca ttttcagggg taaaacgtcg ttttactgag
    11281 aagattattg acgatactgt cattattgat gactttgctc accatcctac tgagattatt
    11341 gcgacattag atgctgctcg acaaaaatac ccgtcaaaag aaattgtagc tattttccaa
    11401 ccgcatacgt tcactcgtac gatagctctt ttagacgatt ttgcccatgc tttgagtcaa
    11461 gcggatagcg tttatcttgc tcaaatatat ggttctgcta gagaagtaga taatggtgag
    11521 gtgaaggtag aagatttagc tgctaagatt gtcaaacact cagatttagt gacagtcgaa
    11581 aatgtctcgc ctttactcaa tcatgataat gctgtctatg tctttatggg tgctggagac
    11641 attcaattgt atgagcgctc ttttgaagaa ttattagcta acctaactaa aaatacacaa
    11701 taagttgata gtaagggctg agagaagttt ctcagtcctc tatctgactt tatcaagaga
    11761 atttcataaa gaaattaagg agatgagtat gattattaga aatggttgcc tagaggattt
    11821 acagcaagtc attagtattg aacgaataaa tttttcagaa gcagaggctg cgagtaaaaa
    11881 agcaatgcaa gaacgtttaa tgataatgac tgatactttt ttagttgcag agattaatgg
    11941 tcgtttagca ggttacattg aaggaccggt tattaaaggt cggtacttaa cggacgattt
    12001 attccataaa gtttctgaaa tccctgtaag ggaaggtggt tttataggaa ttactagtct
    12061 atcaatacat cctgatttta aaggtcaggg gattggtaca gcattattag cagcgatgaa
    12121 agacctggtt gtttctcaag aacgagatgg tataagttta acttgccatg atgatttaat
    12181 ttccttctat gaaatgaatg gatttaaaga tgagggagaa tctgactccc aacatggtgg
    12241 ttcgctttgg tataatatga tttggaataa tccaacacga tcaatttgtt aaaagtagat
    12301 aacattgtca aagttttgtt gattttatag actatttaag atataattgc ctgtgattat
    12361 tattaggagg aaatctcttt gaccgaattt aatgacgatc agcattctaa ccatgatcag
    12421 aaaagtttta aagaacaaat tctggcagag ttagaagaag ctaaccgttt gagaaagttg
    12481 cgtgaagaag aactctatca aaaagagcaa gaagccaaag aagctgctcg cagaacagca
    12541 caattgatgg cagattatga agcgcaacgt ttgaaagacg aacaagaagc tagagcaaag
    12601 gcgctagaga ctaagcaacg cttagaagag caagagaagg ctcggattga ggcgaaactt
    12661 ctggcagaag ctgctcgaga agaagaaaga cgtcaggcgg aacaagcatt agcttcccaa
    12721 gaagaacaag ttatcaatca agggatggaa ccttcaagag agcttgatag cggctccaaa
    12781 tctagtgaat ttaggacaac tgaaaatgta cctgacatag atttaaaagc agataagact
    12841 gatgttgcaa ctgctgtgcc taatcaagaa acggaagaaa tctttcttgt gagagcaaca
    12901 gatattccta cagaaggtga aaatgtaaaa cttggtgaga catcagagtt agagcccgta
    12961 gcaaaggaac ctatccgagt agaggatttg tcaaaagaag aagaggatat agctttgtca
    13021 gccaagaaca aacataacaa gcgtgaaaga cgtcagaaag cagataatgt ggcaaaacga
    13081 attgcacgta ttctgatatc aattatcata cttgtattgc tcttgacagc atttgtaggt
    13141 tatcgttttg tagatagtgc tattaaacct gttgattcca attcaaataa atttgtacaa
    13201 gttgaaattc cgataggatc tgggaataaa ttaattggtc aaattttaga aaaagcagga
    13261 gtcattaaga gcgccacagt ctttaattac tattccaagt tcaaaaacta tagtaatttt
    13321 caaagtggat actacaatct taaaaaaagt atgactttag atcagattgc tgctgaattg
    13381 gaaaaaggcg gaactgcaga accgacaaaa ccagctttag gtaagatatt aattaccgag
    13441 ggttacacta ttaaacagat tgctaaagct attgagtcta ataaaattga tacaaagact
    13501 acaagtacac cttataaagc tgatgatttc ttgaaacttg ttcaagatga aacgtttatt
    13561 aaaaaaatgg tagctaaata tccaaattta ctaggaagtc tacctgataa atcaaaagct
    13621 atttatcaat tggaaggata tttattccca gctacttaca actattataa agatacaacc
    13681 ttagaaggac ttgttgaaga tatgatttca acaatgaata ctaagatggc accttactat
    13741 aacactatta aagcgaagaa tatgtctgta aatgatgttt tgacattatc atctcttgtt
    13801 gaaaaagagg gatcaacaga cgaagatcgc cgtaaaatag caagtgtctt ttataaccga
    13861 ttatcagccg gtcaagctct tcaaagtaac attgcaattt tgtatgctat ggggaaatta
    13921 ggtgataaaa caagtttagc tgaagatgct caaattaata cttcaattaa atcaccatac
    13981 aacatctata ctaatactgg tttgatgcct ggaccagttg atagtccaag tatctcagca
    14041 attgaagcga ctataaaacc tgcttcaaca gattatcttt attttgtcgc tgatgtgaag
    14101 accggaaatg tatactacgc taaagacttt gaaacacaca aggctaacgt tgaaaaatat
    14161 atcaatagtc aaattaacta agcttataaa catggtgcga gtcatcatgt ttatatcttt
    14221 gacttgaaaa aatgaaaaga aaagagaaat aaaatggcag aaaaaactta cccaatgaca
    14281 caagtggaaa aagatcaact tgaaaaagaa cttgaagagt taaaattagt tcgtagacca
    14341 gaagttgtag agcgtatcaa gattgctcgt tcttatgggg acttatcaga aaatagcgaa
    14401 tatgatgcag caaaagacga acaagcattt gttgagggcc aaattcaaat tttagaaaca
    14461 aaaattcgtt atgctgaaat tattgatagt gatgcagttg ctaaagatga ggttgcaatt
    14521 ggtaaaactg tccttgtcca agaagttggt acaaatgata aagatactta ccatatcgtt
    14581 ggtgctgctg gagctgacat tttttcagga aaaatttcga atgaaagccc aattgctcat
    14641 gctttaattg gtaaaaaaac aggagactta gctacaattg aatcacctgc tggtagctat
    14701 caagttgaaa tcattagtgt tgaaaaaaca aattaatagt ataaatcata
//
LOCUS       DBJYSH010000049        14422 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.049, whole genome shotgun sequence.
ACCESSION   DBJYSH010000049 DBJYSH010000000
VERSION     DBJYSH010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 14422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14422
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.049"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            complement(17..667)
                     /locus_tag="ACUBV3_001136"
     CDS             complement(17..667)
                     /locus_tag="ACUBV3_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HHU3847641.1"
                     /translation="MSSVLTLTNILFLLQGFGLTLYISFISILLSMFFGTLLAIMRNS
                     KNPIWKLIASIYIEFVRNVPNLLWIFIIFLVFQMKSVSAGITSFTIFTSAALAEIIRG
                     GLNGVDKGQTEAGLSQGFTYLQVFIIIIFPQAFRKMLPAIISQFVTVIKDTSLLYSVI
                     AIQEIFGKSQILMGRYFEAGQVFTLYAIITAVYFITNFIISSFSRKLSKRWEQATE"
     gene            complement(679..1374)
                     /locus_tag="ACUBV3_001137"
     CDS             complement(679..1374)
                     /locus_tag="ACUBV3_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HHU3847642.1"
                     /translation="MTLLNISPFAISRWGAFFNHFDLFFKGFLYTLGISFGALLLALI
                     LGILSGGLSTSKSKVGKLISRIYVEVFQNTPLLVQMVFVYYGLAIISNGHIMISAFFT
                     AVLCVGLYHGAYISEVIRSGIEAVPKGQTEAALAQGFTANQTMQLIILPQAIRTILPP
                     MTNQVVNLIKNTSTVAIISGADIMFLAKAWAYDTTNYIPAFAGAAIFYFVICFPLASW
                     ARKQEELNKKTYS"
     gene            complement(1387..2187)
                     /locus_tag="ACUBV3_001138"
     CDS             complement(1387..2187)
                     /locus_tag="ACUBV3_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000946176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HHU3847643.1"
                     /translation="MLRRKRLTLYLLSCIFIFLLFYPNSTSANQLSEIKKSGVLKVGV
                     KQDVPNFGYYNAETNQYEGMEIDIAKKIAKSLGVKPVFVPTTAQTREPLMDNGQIDIL
                     IATYTITPERKANYNISKAYYHDEIGFLVRKNSHIKTIKELDGKHIGVAQGATTKVNL
                     EKYAKEHKLKFSYAQLGSFPELAISLYANRIDAFSVDKSILSGYLSPHTTILKEGFNT
                     QEYGIATSKQDKVLIPYVNKLLVSWEKDGSLKHIYQKFKLKPAKVKKE"
     gene            complement(2199..2954)
                     /locus_tag="ACUBV3_001139"
     CDS             complement(2199..2954)
                     /locus_tag="ACUBV3_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HHU3847644.1"
                     /translation="MSLISYKNVNKYYGDYHALRQINLEIEPGQVVVLLGPSGSGKST
                     LIRTMNALESIDDGSLVVNGHELANISSKELVNLRKEVGMVFQHFNLYPHKTVLENIT
                     LAPIKVLKQSKKEAMEIAEKYLKFVNMWERKDSYPSMLSGGQKQRIAIARGLAMHPKL
                     LLFDEPTSALDPETIGDVLSVMQKLANDGMNMVVVTHEMGFAREVADRIIFMADGEIL
                     VDTTDVQDFFDNPTEPRAKQFLRNIINHTSTDL"
     gene            3294..4004
                     /gene="yycF"
                     /locus_tag="ACUBV3_001140"
     CDS             3294..4004
                     /gene="yycF"
                     /locus_tag="ACUBV3_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator YycF"
                     /protein_id="HHU3847645.1"
                     /translation="MKKILIVDDEKPISDIIKFNLTKEGYETATAFDGREALVQYAEF
                     QPDLIILDLMLPELDGLEVAKEVRKTSHIPIIMLSAKDSEFDKVIGLEIGADDYVTKP
                     FSNRELLARVKAHLRRTENIETAVAEESAQNASSDITIGELQILPDAFIAKKRGEEIE
                     LTHREFELLHHLATHIGQVMTREHLLETVWGYDYFGDVRTVDVTVRRLREKIEDTPGR
                     PEYILTRRGVGYYMKSYE"
     gene            3997..5346
                     /gene="vicK"
                     /locus_tag="ACUBV3_001141"
     CDS             3997..5346
                     /gene="vicK"
                     /locus_tag="ACUBV3_001141"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     VicK"
                     /protein_id="HHU3847646.1"
                     /translation="MNNSAANIRSFELALLFLLVFVAVYFVYLAVRDFKMSKDIRLLN
                     WKVRDLIAGNYSDSILIQGDADLVELGESLNDLSDVFRMAHDNLEQEKNRLASILTYM
                     TDGVLATDRSGKIVMINETAQQQFNLAYDEALSMNIVDMLGSGSPYSFQDLVSKTPEV
                     VLNRRDENGEFVTLRIRFALNRRESGFISGLVAVSHDATEQEKEERERRLFVSNVSHE
                     LRTPLTSVKSYLEALDEGALNEEVAPSFIKVSLDETNRMMRMISDLLSLSRIDNEVTH
                     LDVEMTNFTAFMTSILNRFDQIRNQKTVTGKVYEIVRDYPLKSIWVEIDTDKMTQVID
                     NILNNAVKYSPDGGKITVNLRTTKTQMILSISDQGLGIPKKDLPLIFDRFYRVDKARS
                     RQQGGTGLGLSIAKEIVKQHKGFIWAKSEYGKGSTFTIVLPYDKDAVTYEEWEDVED"
     gene            5350..6159
                     /locus_tag="ACUBV3_001142"
     CDS             5350..6159
                     /locus_tag="ACUBV3_001142"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HHU3847647.1"
                     /translation="MSEIGFKYSILASGSTGNCFYIETPQKRLLIDAGLTGKKVTSLL
                     AEINRKPEDLDAILVTHEHSDHIKGVGVLARKYHLDIYANEQTWKVMDERNMLGKVDV
                     SQKHVFGRGKTLTFGDLDIESFGVSHDAVDPQFYRMMKDDKSFVMLTDTGYVSDRMAS
                     LIENADGYLIESNHDIEILRSGSYPWTLKQRILSDKGHLSNEDGSETMIRTIGNRTKH
                     IYLGHLSKENNIKELAHMTMENNLMRADFGVGTDFSVHDTSPDSATPLTRI"
     gene            6162..6530
                     /locus_tag="ACUBV3_001143"
     CDS             6162..6530
                     /locus_tag="ACUBV3_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HHU3847648.1"
                     /translation="MKKIFYISLGFISLGIGIAGIVLPVVPTTPLVLLSAFCFSRSSE
                     KFDIWLRQTKVYKYYAADFVESRSIAPARKKSMIWQIYILMGISIYFAPLMWLKLGLL
                     IGTIVGTYVLFYVVPDKIDE"
     gene            6705..7391
                     /gene="rnc"
                     /locus_tag="ACUBV3_001144"
     CDS             6705..7391
                     /gene="rnc"
                     /locus_tag="ACUBV3_001144"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HHU3847649.1"
                     /translation="MKELRSKLEKDYGIVFANQELLDTAFTHTSYANEHRLLNISHNE
                     RLEFLGDAVLQLLISQYLFTKYPQKAEGDLSKLRSMIVREESLAGFSRLCGFDHYIKL
                     GKGEEKSGGRNRDTILGDLFEAFLGALLLDKGVEVVHAFVNKVMIPHVEKGTYERVKD
                     YKTSLQELLQSHGDVKIDYQVTNESGPAHAKEFEVTVSVNQENLSQGIGRSKKAAEQD
                     AAKNALATLQ"
     gene            7399..10938
                     /gene="smc"
                     /locus_tag="ACUBV3_001145"
     CDS             7399..10938
                     /gene="smc"
                     /locus_tag="ACUBV3_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HHU3847650.1"
                     /translation="MFLKEIEMQGFKSFADKTKVEFDQGVTAVVGPNGSGKSNITESL
                     RWALGESSAKSLRGGKMPDVIFAGTENRKPLNYAQVSVTLDNSDHFIENIADEVRVER
                     RIFRNGDSEYLIDGRKVRLRDIHDLFMDTGLGRDSFSIISQGRVEAIFNSKPEERRAI
                     FEEAAGVLKYKTRKKETQSKLEQTQGNLDRLEDIIYELDMQVQPLEKQASIAKRFLVL
                     DEERQGLHLSILIEDILQHQSDLTTVEEKLLTVREELATYYQQRQSLEDENQSLKQKR
                     HHLSEEIEVKQLALLDVTKLKSDLERQIDLIRLESSQKAEKKEEAGQRLAELEVKAKD
                     CNDQITQKNIELTTLSEKIAQIRSEIVSIESSLERFSTNPDQVIEKLREDFVTLMQEE
                     ADTSNALTALLADIENQKQASQAKSQEIQEVSKNLEVLKSNAKVALERFEAAKKNVRQ
                     LLSHYQDLGQTLQNLEGEYKNQQSILFDHLDEIKSKQARISSLESILKNHSNFYAGVK
                     SVLQAKDQLGGIIGAVSEHLSFDKHYQTALEIALGGSSQHIIVEDESAAKRSIAFLKK
                     NRQGRATFLPLTTIKPRELAQHYLSKLQSSQGFLGIASELVTYDQRLSNIFKNNLGLT
                     AIFDTVDNANVAARQLNYQVRLVTLDGTELRPGGSYSGGANRQNNTVFIKPELDNLKK
                     ELKQAQSKQLIQEKEVAALLEQLKEKQETLAQLKNDGEQARLEEQRADIEYQQLSEKL
                     ADLNKLYNGLQLSSGALEQTTSENEKNRLEKELEQFAIKKEELTTSIAQIKEDKDSIQ
                     EKVNNLTTLLSEAQLEERDLLNEQKFERANCTRLEITLSEIKRDISNLQTLLSHQDSQ
                     LDKEELPRIEKQLLQANKRRENDEEKLVSLRFELEDCEAALDDLAASLAKEGQKNESL
                     IRQQAQLESQCEQLSQQLMIFSRQLSEDYQMTLDEAKVKANVLEDILMAREQLKSLQA
                     KIKALGPVNIDAIAQFEEVHERLTFLNTQRDDLVHAKNLLLETITDMDDEVKTRFKST
                     FEAIRHSFKETFVQMFGGGSANLILTEGDLLSAGVDISVQPPGKKIQSLNLMSGGEKA
                     LSALALLFAIIRVKTIPFVILDEVEAALDEANVKRFGDYLNRFDKSSQFIVVTHRKGT
                     MSAADSIYGVTMQESGVSKIVSVKLKEAQEMTN"
     gene            11029..11826
                     /locus_tag="ACUBV3_001146"
     CDS             11029..11826
                     /locus_tag="ACUBV3_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HHU3847651.1"
                     /translation="MIKLVATDMDGTFLDENGTYDKKRLANVLKKFKEQGIVFTAASG
                     RSLLSLEQLFADFRDQMAFIAENGSAAVLFNRLAYEQHLSREQYLDIIDHLSKSPYME
                     NNEYVLSGKDGAYILSDANPDYIEFITHYYDNLQKVSHFEDVDDIVFKVTANFTEETV
                     RQAEEWVNQAIPYATAVTTGFKSIDIILSSVNKRNGLEHLCEQYGIRAEEVLSFGDNI
                     NDLEMLEWSGKAIATENARPEVKEIADCIIGHHNDQAVMAYLESMVD"
     gene            11826..12650
                     /locus_tag="ACUBV3_001147"
     CDS             11826..12650
                     /locus_tag="ACUBV3_001147"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HHU3847652.1"
                     /translation="MLDIKILALDLDGTLFTTDKKVSEENKVALKAAREKGIKVVITT
                     GRPLKAIGNLLEDLELVSDEDYSITFNGGLVQQNTGKILAKTAMTRQEVEDIHEELYQ
                     VGLPTDILSEGTVYSIANKGHHSQYHLANPLLEFIEVDDLEQVPKDVVYNKIVSVIDA
                     TYLDQQIAKLPDRLKVDYEMFKSRDIILELMPKGVHKAVGLELLTKHLGLDSSQVMAM
                     GDEANDLSMLEWAGLGVAMANGIPEAKATAKATTICNNDESGVAEAVGKYILSEEN"
     gene            12650..14260
                     /gene="ftsY"
                     /locus_tag="ACUBV3_001148"
     CDS             12650..14260
                     /gene="ftsY"
                     /locus_tag="ACUBV3_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HHU3847653.1"
                     /translation="MGLFDRLFGHKKKDKELEIEASESVVLENEDSVIDKEESSNFSK
                     ESTLNRTSEVPVAEDDSFLELERDTALSESHQPVTSEIHPLESEDTSEIPVKEDDSFL
                     ELEDRAKTKVADTSEVGNVVPDSTTLSDNVSAKSEASFSDEEQSSDSQASEQFSETPL
                     QEEMSSGKTEVQTESEDTSAADAFLADYYAKRKAIEKEISSNSLSTDESELSEAQEVL
                     SQSQADTIKAESQEEKYNRSLKKTRTGFSARLNAFLSNFRRVDEEFFEELEEMLILSD
                     VGVNVATQLTEDLRYEAKLENAKKSEDLKRVIVEKLVEIYEKDGIYNEAINFQEGLTV
                     MLFVGVNGVGKTTSIGKLAHQYKSQGKKVMLVAADTFRAGAVAQLVEWGRRVDVPVVT
                     GEKKADPASVVFDGMEKAVAQGVDVLLIDTAGRLQNKENLMAELEKIGRIIKRVVPDA
                     PHETLLALDASTGQNALSQAKEFSKITPLTGLILTKIDGTAKGGVVLAIRQELDIPVK
                     FIGFGEKIDDIGEFNSEDFMRGLLEGIL"
BASE COUNT         4867 a         2234 c         2936 g         4385 t
ORIGIN      
        1 aaggccttcg ttttaattac tcagtagctt gctcccaacg ttttgaaagt ttacgtgaaa
       61 aacttgagat gataaagttt gtaatgaaat agacagcagt tataatagcg tagagtgtaa
      121 aaacttgtcc agcttcgaaa tatcttccca ttagaatttg actttttccg aagatttctt
      181 gaatagctat aacagaataa agtagtgatg tatcttttat aaccgttaca aactgagaaa
      241 taatggctgg caacattttg cgaaaagctt gtggaaaaat aataataatg aaaacttgca
      301 aataagtgaa tccttgagaa agtccagctt ctgtctgtcc tttatcaact ccatttaaac
      361 ctccccgaat aatttctgct aaagcagcgg aggtaaaaat tgtaaacgaa gtaataccag
      421 cgctgactga tttcatctgg aatactaaga aaatgataaa aatccaaagt agatttggaa
      481 catttctgac aaattcgata tagatactag ctattaattt ccagatagga tttttgctat
      541 ttctcataat tgctaataga gttccaaaaa acattgatag taaaatggaa ataaaagata
      601 tatataaggt taaaccgaac ccttgaagga gaaatagtat atttgttagt gttaagactg
      661 atgacatatc aaacctcctt aagaataagt tttcttattt aattcttctt gttttcttgc
      721 ccagcttgct agtgggaaac aaataacaaa atagaagata gcagctcctg caaaagctgg
      781 gatataatta gtcgtatcat aagcccaggc cttagctaaa aacatgatat cggctccaga
      841 aatgattgct acggtagagg tattcttgat taggttaaca acttggttgg tcattggtgg
      901 taaaattgtt ctgatagctt gaggcaaaat gataagttgc atggtttgat tagctgtgaa
      961 tccttgagct aaagcagcct ctgtttgtcc tttagggaca gcttcaattc ctgagcgaat
     1021 aacttcagaa atataggcac catggtatag gccaacgcac aaaacagcgg tgaaaaaagc
     1081 actaatcata atatgaccat tactaatgat tgctagtcca tagtagacaa aaaccatttg
     1141 aaccaaaaga ggtgtgtttt gaaaaacttc aacatagata cgtgatatca acttaccaac
     1201 tttactttta gaagttgata aaccaccaga gagaattcct aatatcagag caagtaggag
     1261 tgcaccaaac gaaataccta gagtatataa gaagccttta aagaataggt caaaatggtt
     1321 aaagaaagcg ccccaccttg aaatggcaaa aggactgatg tttaataaag tcataaaaaa
     1381 tcctccctat tcttttttta ctttagcggg tttaagctta aatttttgat aaatgtgttt
     1441 taaactacca tctttttccc atgataccag taacttatta acgtagggaa tcaagacttt
     1501 gtcctgttta ctggttgcaa taccgtattc ttgcgtattg aacccttctt ttaatatggt
     1561 tgtatgaggg ctaaggtagc cgctaaggat agatttatca actgaaaaag catcaatacg
     1621 attagcgtac agcgatatcg ctaattctgg gaaactccct aattgagcat agctaaattt
     1681 tagcttgtgt tctttagcat atttttctaa attgactttt gtagtggcac cttgagccac
     1741 accaatgtgt ttaccatcca attcttttat agttttgata tgactatttt tacgcaccaa
     1801 aaatccaatt tcatcgtgat aataagcttt agaaatatta tagttagctt ttctttcggg
     1861 cgtgatagtg tatgttgcga ttagaatatc aatttgccca ttgtccatca gaggctctct
     1921 ggtttgtgca gttgtcggta caaaaactgg tttgacgcct aaggatttgg caattttttt
     1981 ggcaatatca atttccattc cttcgtattg attcgtctca gcattataat aaccaaagtt
     2041 aggcacatct tgcttgactc caactttaag gacacccgat tttttgattt ctgataattg
     2101 attagctgac gttgagttgg gataaaataa caggaaaatg aaaatacagg ataatagata
     2161 gagcgttaat ctttttcgtc tcagcatatt tcgtcctcct aaaggtcagt actggtatgg
     2221 ttgataatgt ttcttaaaaa ttgtttggca cgtggttctg tgggattatc aaaaaagtct
     2281 tgcacatccg tagtatctac taaaatttcc ccgtcagcca taaaaataat acgatcagca
     2341 acttctcgtg cgaatcccat ttcatgagtt acaacgacca tattcattcc gtcatttgcc
     2401 aatttttgca tcactgaaag aacgtctccg atggtttcgg gatcaagggc agaggtaggc
     2461 tcatcaaaga ggaggagttt tggatgcatg gcaagccctc tagcaatagc aattctttgt
     2521 ttttgaccac ctgatagcat agatgggtag gaatcttttc tttcccacat attgacaaat
     2581 tttaaatatt tttcagcaat ttccatagct tcttttttag attgttttaa tactttaata
     2641 ggagctaatg taatattttc taacacggtt ttgtggggat aaaggttaaa atgttgaaaa
     2701 accataccaa cttctttacg gaggttgacc aattctttag atgaaatatt tgctaattca
     2761 tgtccattca cgaccaaact accatcgtca atagattcga gagcgttcat tgttctaatg
     2821 agtgttgatt tacctgatcc agaaggtcct aatagaacaa ccacttgccc tggctctatt
     2881 tccaaattaa tctgacgaag ggcgtgataa tctccataat acttgttaac attcttataa
     2941 gatatgagtg acataagaac ctcctaaaat aacagtttat tgttaggtta cctaacatta
     3001 tagataagtg tatcacctgt ttgtgagctt gtcaataaat tcagaatatt aaagagtagg
     3061 attgatttaa acattgctat ttttagagcg gtatttctag taaaaatatt acgttctcgt
     3121 atcatttatt tttcagttta ataggatgta ttgaaagttt ccaattaact aggaaatctg
     3181 ttataattag tgataatagt ggtgctttag gcccatatta tcatattttc ggtcactttt
     3241 atttttcagt ttatggatgg aagtgggaga ccagaaagta atagaggtaa aatatgaaaa
     3301 aaatacttat cgttgatgat gaaaaaccaa tttcagatat tatcaaattt aatttaacca
     3361 aagaaggata tgaaacggcg acagctttcg atggccgtga agctttagta caatatgcag
     3421 aatttcaacc agatttaatc attttggatt taatgctccc tgaactagat ggtttagaag
     3481 tggctaagga agttcgtaaa accagccata ttcctattat tatgctttcc gcaaaagaca
     3541 gtgagtttga caaagtcatt ggtttagaaa tcggtgcaga tgattatgtt actaaacctt
     3601 tttcaaatcg tgaacttttg gcacgggtca aagcacattt acgacgtact gaaaatattg
     3661 aaacagctgt agcagaagaa agtgctcaaa atgcttcttc agatattact attggtgagt
     3721 tgcaaatttt accagacgcc tttattgcta agaaacgtgg cgaagaaatt gaattaacgc
     3781 accgagaatt tgaattactt caccatttag cgacacatat aggacaagtc atgacacgcg
     3841 aacatttatt ggaaacggtt tggggatatg attattttgg agatgtccgt acagttgatg
     3901 tgacggtgag acgtttacgt gaaaagattg aagatactcc tggtcgtcca gaatatattt
     3961 tgactcgacg tggtgtaggt tattacatga agtcttatga ataatagtgc tgcaaatatt
     4021 aggtcgtttg agctagcctt acttttttta ctagtttttg ttgctgtcta ttttgtttat
     4081 ctagcggtta gagattttaa aatgtctaaa gatatccggc ttttgaattg gaaagtcaga
     4141 gatttgatag caggaaatta ttcagatagt attctaattc aaggtgatgc agatttggta
     4201 gaattagggg aaagtctaaa tgatttatct gatgtcttcc gaatggcaca tgataactta
     4261 gaacaagaaa agaatcgctt agcgagtatc ttaacttata tgactgacgg tgtgctggct
     4321 acagatcgtt caggaaaaat cgttatgatt aatgagacag cccaacagca gttcaatcta
     4381 gcatatgatg aagctttatc aatgaatatt gttgatatgt tagggtcggg aagtccatat
     4441 tcgttccaag atttagtctc taagacgcca gaggtagttc ttaatagacg tgatgaaaat
     4501 ggtgaatttg ttaccttacg gattcgattt gctttgaacc gtcgtgaaag tggttttatt
     4561 tccggtttag ttgcagtatc acacgatgct actgagcaag agaaagaaga gcgtgaaaga
     4621 agactttttg tatctaatgt ttctcatgaa cttcgtacac ctttgacatc tgtaaaatct
     4681 tatttagaag ctttagatga gggagcgctc aatgaagaag ttgctccaag cttcattaag
     4741 gtatctctag atgaaacaaa tcgtatgatg cgtatgatat cagatttatt aagtttatcg
     4801 cgcattgata atgaagtaac gcatttagat gttgaaatga cgaattttac agctttcatg
     4861 acctcaattt tgaatcgatt tgatcagatt agaaatcaaa aaacagtcac aggaaaagtt
     4921 tatgaaattg tcagagatta tcctcttaag tcaatttggg tggaaattga tacagataag
     4981 atgactcaag tgattgataa cattttaaat aatgcagtca agtattcacc agatggtggt
     5041 aagattacag ttaatctacg cacaactaaa acgcagatga ttttatcaat atcagaccaa
     5101 ggcttaggta ttcccaaaaa agatttacct ctcatttttg atcgttttta tcgtgttgat
     5161 aaggcgagaa gtcgtcaaca gggtgggact ggacttggtt tgtcaattgc aaaagaaatt
     5221 gttaagcagc ataagggatt tatttgggct aagagtgagt atggtaaagg gtctactttt
     5281 acaatcgtct tgccttatga taaagatgct gtaacttatg aagaatggga ggacgttgaa
     5341 gattaaaaca tgtcagaaat aggatttaaa tatagtatct tagcttcagg ttcaactgga
     5401 aattgttttt atattgagac accccaaaaa cgtcttttaa ttgacgctgg tttaacaggc
     5461 aagaaagtga ctagccttct tgcagaaatt aatcgtaaac cagaagattt agatgctata
     5521 ctagttaccc atgaacattc tgaccacatt aaaggtgttg gcgtattggc taggaaatat
     5581 caccttgata tttatgccaa tgagcagacg tggaaagtta tggatgagcg taacatgtta
     5641 ggcaaagttg atgttagtca aaagcatgtt tttggacgcg gtaaaacctt aactttcggg
     5701 gatttagata ttgagagctt tggtgtaagt catgatgctg tggatcccca attttaccgt
     5761 atgatgaaag atgataagag ctttgtgatg ttgacagata caggatatgt tagcgaccga
     5821 atggcaagct tgattgagaa tgcagatggt tatttgatcg agtcaaatca cgacattgag
     5881 attctacgtt cgggttctta tccttggaca cttaagcaac gtattttatc agataaagga
     5941 catttatcta atgaagatgg ttcagaaacc atgattcgca ctataggtaa ccgtactaaa
     6001 cacatctatt taggacactt gagtaaggaa aataatatta aggaacttgc tcacatgact
     6061 atggaaaaca atttaatgag agctgacttt ggtgtgggca cagatttttc tgttcacgat
     6121 acttcacctg attcagctac cccactgacg aggatttaag attgaaaaag attttttaca
     6181 ttagtttagg atttattagt ttagggatag gcattgcagg tattgtcttg ccagtagttc
     6241 caacgactcc attagtattg ctttcagctt tttgcttttc gagaagctct gaaaaatttg
     6301 atatttggtt gcgtcaaact aaagtttata aatactacgc tgcagatttt gttgaaagca
     6361 gaagtattgc cccagcgcgt aaaaagtcaa tgatttggca aatttatatc ttgatgggga
     6421 tttcaatata ttttgctcct ctaatgtggc tcaaactggg attattgatt ggaactattg
     6481 taggaactta tgtgcttttt tatgtagtac ctgataaaat tgatgaatag ttaacaagag
     6541 actaggtgtg tatcctagtc ttttgttgaa tattcaaata tgtaattaag gaaacgtttt
     6601 ctttggaaga taagtttaac ttccaaaatg tttgattatt tgtcaactgt ggtataatgg
     6661 tatttgttta atattttatc tatgattgaa atcaaggaga aaaaatgaaa gaattaagat
     6721 caaagctgga aaaagactat ggtattgttt ttgctaatca agaattatta gatacagctt
     6781 ttacccatac atcatacgca aatgagcatc gcctcctaaa catttcacat aatgaacgtt
     6841 tggaattttt aggagacgct gttctacaat tacttatttc acagtatctt tttacaaagt
     6901 accctcaaaa ggcagaaggt gatttatcaa aattacgttc aatgattgtt cgtgaggaaa
     6961 gtcttgcagg tttttcacgt ctatgtggct ttgatcacta tattaaactt ggaaaaggag
     7021 aagaaaaatc tggtggacgt aatcgtgaca ctattttagg agatttattt gaggcttttt
     7081 taggagcttt attattggat aaaggtgttg aagtggttca tgcctttgtt aacaaggtaa
     7141 tgatccctca cgttgaaaaa gggacctatg agcgtgttaa agattacaag acaagcttgc
     7201 aagaattact tcaatctcat ggggatgtaa aaatagatta tcaagttact aatgaaagtg
     7261 gtccagcaca tgcaaaggaa tttgaggtga ctgtatctgt caaccaagaa aatttgagcc
     7321 aaggaattgg acgatcaaag aaagctgctg aacaagacgc tgccaaaaat gctttagcaa
     7381 cgttacaatg aggaaatcat gtttttaaaa gaaattgaaa tgcaaggctt taagtccttt
     7441 gcggataaaa ctaaggtaga atttgatcaa ggagttactg ctgttgtagg tccaaatgga
     7501 tcaggtaaaa gtaatatcac agagagttta cgttgggcat tgggagaatc aagcgcaaaa
     7561 agtttacgag gaggtaagat gccagacgtc atatttgctg gtacagaaaa tcgtaagccc
     7621 cttaattatg cgcaagtgag tgttacttta gataacagcg accactttat cgaaaatata
     7681 gctgacgaag ttcgagttga acgtcgtatt tttcgaaacg gagatagtga atatttaata
     7741 gatggacgca aagttcgctt acgtgatatt catgatttat ttatggatac tggattaggg
     7801 agagattctt tttctattat ttcccaagga cgcgttgaag ctatttttaa tagtaaacca
     7861 gaagagcgcc gtgccatatt tgaagaagcc gctggtgttc tgaaatacaa aacacgtaag
     7921 aaagaaacac aatccaaatt agaacaaact caagggaact tagatcgctt agaggatatt
     7981 atttatgaat tggacatgca agtccaacca ttagaaaaac aagcaagtat tgctaagcgt
     8041 tttttagtat tagatgagga acgacaagga ctccacttat ctattttgat tgaagatatt
     8101 ttacaacatc aatcagattt aactactgtt gaggaaaagc tattgacagt aagagaagag
     8161 ttagcaactt attaccaaca acgtcagtcc cttgaagatg aaaatcaatc gttaaaacag
     8221 aaacgtcacc atttatctga agaaattgag gttaaacagt tagctctttt agacgttaca
     8281 aagttaaagt cagatttgga acgccaaatt gatctgattc gtttagagtc aagccaaaag
     8341 gctgaaaaga aagaagaggc aggtcaacgc ttagcagagt tagaagttaa agctaaagat
     8401 tgtaatgacc agattaccca aaaaaatata gagttaacta ctctgtcgga aaaaatagcc
     8461 caaataagat cagaaattgt tagtatagaa agttccttag agcgtttttc aactaatccg
     8521 gatcaggtta ttgaaaaact tcgagaagac tttgttacct tgatgcaaga agaagccgac
     8581 acttcaaacg ctttaacagc tttactagct gatatagaga atcaaaaaca agcttctcaa
     8641 gctaaatctc aagaaataca agaagttagc aaaaatttag aagtattaaa aagtaacgca
     8701 aaggtagcat tggaacgatt tgaggctgca aagaagaatg ttcgtcagtt attatctcac
     8761 taccaagatt taggtcaaac cttgcaaaat ctagaaggtg agtataagaa tcagcagtca
     8821 attttatttg atcatttaga tgaaatcaaa tcaaagcaag cacgtatttc gagtttagaa
     8881 tctattttga aaaatcatag taatttctat gctggagtga agtcagttct acaggctaag
     8941 gaccagcttg gaggtatcat aggtgctgtt agtgagcatc ttagctttga caaacattac
     9001 caaacagcat tggagatagc tttgggaggt agcagccaac acatcattgt tgaagacgag
     9061 agtgctgcca aacgttcaat tgctttttta aagaaaaacc gccaaggtag agcgacattt
     9121 ctgcctttaa ctacaataaa accaagagaa ttagcacaac attatctctc aaaattacaa
     9181 agttctcaag ggtttttagg aatagctagt gaattggtaa cctatgatca acgcttgtca
     9241 aacattttta aaaataactt agggttaact gctatttttg atacggttga taatgctaat
     9301 gtagctgcaa gacaattgaa ttatcaagta cgtttagtaa ccttagatgg aacagagtta
     9361 cgccctggag ggtcgtactc agggggagca aatcgtcaaa ataatactgt ttttatcaag
     9421 cctgaattgg ataatctaaa aaaagaattg aaacaggcac aaagtaaaca gttaattcaa
     9481 gaaaaagaag ttgccgcatt gttagagcaa ctcaaagaga aacaagaaac cttagcacaa
     9541 cttaagaatg atggagaaca agctcgtctt gaggaacaaa gagctgatat agagtatcaa
     9601 caactatcag aaaagcttgc tgatttaaat aaactatata atggcttgca attaagcagc
     9661 ggggcgttgg aacaaactac ttctgaaaat gaaaaaaatc gtttagaaaa agaacttgaa
     9721 caatttgcta ttaaaaaaga agagttgaca acttcaattg ctcaaattaa ggaagataaa
     9781 gattcaattc aagaaaaagt caataatcta acaacattat tgtcagaggc acagctagaa
     9841 gaacgtgatt tgctaaacga gcagaaattt gaacgtgcca actgtacacg tctagagatt
     9901 accttgtctg aaataaaacg tgacattagt aatttacaaa cgttactaag ccatcaggac
     9961 agtcaattag acaaagaaga gcttccacgt atagaaaaac agttgctaca agcaaataag
    10021 agaagagaaa atgacgaaga aaagcttgtg agtctccgtt ttgaattgga ggattgtgaa
    10081 gctgctttag atgatttagc tgcttctctg gctaaagaag gacaaaaaaa tgaaagcctt
    10141 attcgtcagc aagctcaatt agagtcacaa tgtgaacaat tatctcaaca attgatgata
    10201 ttctcacgtc aattatcaga agactatcag atgacattgg atgaagcgaa ggtaaaagca
    10261 aatgtcttgg aagatatttt gatggcgcgt gaacaattga aatctctaca agctaaaatt
    10321 aaagctttag gacctgttaa tattgatgca attgctcaat ttgaagaagt tcatgaacga
    10381 ttgaccttcc ttaacacaca gcgagacgac ttagttcatg ccaaaaattt attactagag
    10441 acgattacag atatggatga tgaagttaag actcgtttta aatcgacatt tgaagccatt
    10501 cgtcatagtt tcaaagaaac ctttgtacag atgtttggtg gaggttctgc gaaccttatt
    10561 ctgacagaag gtgatttatt atcagctggg gttgatattt cggttcaacc tcctggtaaa
    10621 aaaatccagt ctttaaactt gatgtcaggt ggtgaaaaag cattatcagc gttagcatta
    10681 ttatttgcca ttatccgtgt taagacaatt ccatttgtca tcttggatga agttgaggcc
    10741 gctttagatg aagcaaatgt taagcgattt ggcgattatc ttaatcgatt tgataaatca
    10801 agtcaattta tcgttgttac tcaccgaaaa gggactatgt ctgctgctga tagtatctat
    10861 ggggtaacga tgcaggaatc aggcgtttcc aaaattgttt ctgtaaaatt aaaagaagct
    10921 caagaaatga ccaattagaa aacagaaggg gatcccttct gttttttgga ttttcagcct
    10981 tattttatgt tacaatagta aatgtaaacc ctttagttag gagattccat gataaaatta
    11041 gtagcaaccg atatggacgg tacattctta gatgaaaacg gtacctatga taaaaaacgt
    11101 ttagcaaatg tgctaaaaaa atttaaagaa caaggaattg tctttacggc agcaagtggg
    11161 cgttccttat tatcacttga acaattattt gctgactttc gtgatcagat ggcttttata
    11221 gcagaaaacg gaagtgcagc agtcttattt aatcgcttgg catatgaaca gcacttgagt
    11281 cgagaacaat acttagatat tattgaccat ttgagtaaga gcccttatat ggagaataat
    11341 gaatatgtct tatctggcaa ggatggtgca tatattctaa gtgatgccaa tccagattat
    11401 attgaattca tcactcatta ttatgataat ttgcaaaaag tttctcactt tgaagatgta
    11461 gatgatattg ttttcaaagt aacggctaac tttactgaag agactgttag acaagctgag
    11521 gagtgggtaa atcaagctat accgtatgcc acagcagtga caacaggttt taaatccatt
    11581 gatattattc taagctcagt taataaacga aatggtttag agcacctctg tgaacaatac
    11641 ggtattagag cagaagaagt cttatctttt ggtgacaata ttaatgattt agaaatgcta
    11701 gagtggtcag gaaaagctat cgcaacagaa aatgctagac cagaagttaa agagatagca
    11761 gattgtatta taggtcatca caatgatcaa gctgtaatgg cttatttaga aagtatggtg
    11821 gattaatgtt ggatattaaa atacttgcct tagatttaga tggcacttta tttacaacag
    11881 ataagaaagt ttcagaagaa aataaagtgg ccttaaaagc agctagagaa aaaggtataa
    11941 aagttgttat tacaacaggg cgtcctttaa aagcaattgg taatttgtta gaggacctag
    12001 agttagtatc tgatgaagat tacagcatta cttttaacgg tggtttagtt caacaaaata
    12061 cagggaaaat cttagctaaa actgcaatga cacgacaaga agtggaagat atccatgagg
    12121 agttgtatca agtgggtttg ccaacagata tcttaagtga gggaacggta tatagtattg
    12181 ctaacaaggg acaccattct caatatcatt tagccaaccc actcttagaa tttatagaag
    12241 ttgatgattt agagcaagtg ccaaaggatg tagtttataa taaaattgtt agtgttattg
    12301 atgctacata tcttgatcaa caaattgcta aacttcctga tagacttaaa gttgactatg
    12361 aaatgtttaa atcacgtgat atcattttag aattgatgcc taaaggtgtt cataaagcag
    12421 tcggcttaga actactgaca aaacaccttg ggttagatag tagtcaagtt atggcaatgg
    12481 gagatgaagc aaatgattta tcaatgctag agtgggcagg cttaggagtt gcaatggcta
    12541 atggtattcc agaagctaag gcaacagcaa aagcgacaac tatttgtaat aacgatgagt
    12601 ctggagttgc cgaagcagtt gggaaatata ttttaagtga ggaaaattaa tgggattatt
    12661 tgaccgccta tttggacata agaaaaaaga taaagaacta gaaattgaag catcagaatc
    12721 tgttgtttta gaaaatgaag attcagttat tgataaagag gaaagcagca acttttcgaa
    12781 ggagtcaact ttgaatcgaa cctctgaagt ccctgtcgca gaagatgatt ctttcttaga
    12841 attagaaaga gatacagcat tgtcagaatc acatcaacca gttacctctg aaattcatcc
    12901 tttagagtca gaagatacct ctgaaattcc tgtaaaagaa gacgatagtt ttttagaact
    12961 agaagatagg gctaagacaa aggtggcaga tacttctgag gtaggaaatg tagttcctga
    13021 ttccacgact ctttctgaca atgtttctgc taaatctgag gcatctttta gtgatgagga
    13081 gcaatcaagt gatagtcagg cttcagagca gttttcagaa actccccttc aagaagagat
    13141 gtctagtgga aaaactgagg tacaaacaga gtcagaggat acatctgcgg ctgatgcttt
    13201 tttagcagat tactatgcta aacgtaaagc aattgagaaa gaaatctcgt ctaattcgct
    13261 gtcaacagat gagtcagaac tttctgaggc acaggaagtt ttatcgcaat cacaggcaga
    13321 cactataaaa gctgagagtc aagaggaaaa atataatcgt agcctgaaaa aaacacgcac
    13381 aggttttagt gcacgcctta atgctttcct ttctaacttc cgtcgtgttg acgaagagtt
    13441 ctttgaagaa ttagaagaaa tgttaatctt atctgacgtt ggggtaaatg ttgcaacaca
    13501 gttaactgaa gaccttcgtt atgaagcaaa attagagaat gccaagaaat cagaggattt
    13561 aaaacgtgtt atcgttgaaa aattagttga aatctacgaa aaagatggta tttataatga
    13621 ggctatcaat ttccaagaag gtttaaccgt aatgcttttt gtaggtgtta atggtgttgg
    13681 aaaaacgaca tctataggta agttagctca tcaatacaag tctcaaggga aaaaagttat
    13741 gttagtagca gctgatactt tccgtgcagg tgctgtggct caactagtag aatggggacg
    13801 ccgtgttgat gtaccagttg tcactggaga aaaaaaagca gatccggcta gcgttgtctt
    13861 tgatggcatg gaaaaagccg ttgctcaagg cgttgatgtc ttgttaattg atacagcagg
    13921 acgtttgcaa aataaagaaa accttatggc agagttggaa aaaattggac gtattattaa
    13981 gcgtgtcgta ccagatgctc cccacgaaac tttgcttgct ttagatgcat ctacaggaca
    14041 aaatgcttta agtcaagcta aagagttctc gaaaattaca ccattaactg gccttattct
    14101 gactaaaatt gatggtactg ctaagggtgg tgtggtactg gctattcgtc aagaactaga
    14161 tattcctgtt aaattcattg gtttcggcga aaaaatcgat gatattggtg aatttaattc
    14221 agaagatttc atgcgtggcc ttctggaagg aattttataa gaaaaaagaa ctttcattta
    14281 gaaagttctt tttatagtac gacgggcatg tcgtacatct gaggtgcaag tcctctgtgg
    14341 gcacccgcta ccggtgaacc caatagcgac tcccaagctt gactatcgtg aggtagcggg
    14401 gagaggaagg gatagcgaaa tc
//
LOCUS       DBJYSH010000050        14284 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.050, whole genome shotgun sequence.
ACCESSION   DBJYSH010000050 DBJYSH010000000
VERSION     DBJYSH010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 14284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14284
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.050"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            3..1124
                     /gene="dnaN"
                     /locus_tag="ACUBV3_001149"
     CDS             3..1124
                     /gene="dnaN"
                     /locus_tag="ACUBV3_001149"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HHU3847654.1"
                     /translation="MNKNFFLHALTVTKRAISHKNAIPILSTVKIEVTRDAIILTGSN
                     GQISIENTIPASNENAGLLVTNPGSILLEAGFFINIISSLPDVTLEFTEIEQHQIVLT
                     SGKSEITLKGKDVDQYPRLQEMTTDTPLTLETKLLKSIINETAFAASQQESRPILTGV
                     HLVISQNKYFKAVATDSHRMSQRTFQLEKSANNFDLVVPSKSLREFSAVFTDDIETVE
                     VFFSDSQMLFRSENISFYTRLLEGNYPDTDRLLTNQFETEIIFNTNALRHAMERAYLI
                     SNATQNGTVRLEIQNETVSAHVNSPEVGKVNEELDTVSLKGDSLNISFNPTYLIESLK
                     AVKSETVTIRFISPVRPFTLTPGEDTEDFIQLITPVRTN"
     gene            1182..2075
                     /locus_tag="ACUBV3_001150"
     CDS             1182..2075
                     /locus_tag="ACUBV3_001150"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol/lipid kinase family protein"
                     /protein_id="HHU3847655.1"
                     /translation="MMSNMTLYIIANPHAGNKNASTIVGKIQELYHTEDISVFYTEQK
                     DDEKKQVINILRSFKESDHLMIIGGDGTLSKVMTYLPQHIPCAYYPVGSGNDFARALK
                     IPNLKETLTAIQTERLKEINCFIYDKGLILNSLDLGFAAYVVWKASNSKIKNILNRYR
                     LGKITYIVIAIKSLLHSSKVQVLVEGETGQQIKLNDLYFFALANNTYFGGGITIWPKA
                     SALTAELDMVYAKGHTFLKRLSILLSLVFKRHTTSKSIKHQTFKAMTVYFPKNSLIEI
                     DGEIVELDQISLKCQKRYLYM"
     gene            2085..2282
                     /locus_tag="ACUBV3_001151"
     CDS             2085..2282
                     /locus_tag="ACUBV3_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HHU3847656.1"
                     /translation="MYQVGSLVEMKKPHACVIKETGKKANQWKVLRVGADIKIQCTNC
                     QHVIMMSRYDFERKLKKVLQP"
     gene            2448..2687
                     /locus_tag="ACUBV3_001152"
     CDS             2448..2687
                     /locus_tag="ACUBV3_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000369332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HHU3847657.1"
                     /translation="MSKWENGDTTPDLDNLIRLAEIFDVSLDEHVLAKTNEVKVERIY
                     ENKPLDLRKYNKIYWFIFRNIIRSLLIILAILTFL"
     gene            2847..3962
                     /gene="ychF"
                     /locus_tag="ACUBV3_001153"
     CDS             2847..3962
                     /gene="ychF"
                     /locus_tag="ACUBV3_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HHU3847658.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDERLQKLTELITPKKTVPTTFEFTDIAGIVKGASKGEGLGNKFLANIREVDAIVH
                     VVRAFDDENVMREQGREDAFVDPIADIDTINLELILADLESINKRYARVEKMARTQKD
                     KESVAEFNVLQKIKPVLEDGKSARTIEFTEEEAKVVKGLFLLTTKPVLYVANVDEDKV
                     ADPDDIDYVNQIRAFAETENAEVVVISARAEEEISELDDEDKLEFLEAIGLTESGVDK
                     LTRAAYHLLGLGTYFTAGEKEVRAWTFKRGIKAPQAASIIHSDFERGFIRAVTMSYDD
                     LIQYGSEKAVKEAGRLREEGKEYIVQDGDIMEFRFNV"
     gene            4046..4621
                     /gene="pth"
                     /locus_tag="ACUBV3_001154"
     CDS             4046..4621
                     /gene="pth"
                     /locus_tag="ACUBV3_001154"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - peptidyl-tRNA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HHU3847659.1"
                     /translation="MVKMIVGLGNPGSKYNDTKHNIGFMAVDRIVKDLDVNFTEDKNF
                     KAEIGSDFINGEKIYFIKPTTFMNNSGIAVKALLTYYNISIKDMIIIYDDLDMEVGKI
                     RFRQKGSAGGHNGIKSIIAHLGTQEFDRIKVGIGRPNGRMTVINHVLGKFDKNDEIMI
                     LNTLDKVDNAVNYYLQTNDFQKTMQKYNGLK"
     gene            4618..8115
                     /gene="mfd"
                     /locus_tag="ACUBV3_001155"
     CDS             4618..8115
                     /gene="mfd"
                     /locus_tag="ACUBV3_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HHU3847660.1"
                     /translation="MNIIELFSQNKVVWTWHSGLVTNSRQLVMGFSGASKAIAIASAY
                     EKLSKKIMVVTATQTDSDKLSSDISSLIGEDNVYQFFADDVPAAEFIFSSLDKSISRL
                     SALRFLKDPEKNGVLITSISGLRLLLPNPEVFSKSQYKFEIGQECYLDKLCKNLVNLG
                     YQKVSQVFSPGEFSQRGDILDIFEMTQEYPYRLEFFGDEIDGIRQFDIDTQKSLKQLE
                     SVQISPADDIILQDADFERAKKKLEGYLVTASEGQRTYLSEVLSTTENHFKHSDIRRF
                     LSIFYEKEWGILDYIPEGTPLFVDDFQKIVDRNAKLDLEIASLLTEDLQQGKSHSSLN
                     YFSDPYKQLRQYQPATFFSNFHKGLGNLKFDKLHHFTQYGMQEFFNQFPLLVDEINRY
                     KKSGATVLLQVDSQKGLNLLQENLKEYGLDLIISDKNDIVQKESQLIVGHLSNGFYFA
                     DEKIVLITEREIYHRRVKRKIRRSNISNAERLKDYNELSVGDYVVHNVHGVGKFLGIE
                     TIEIQGIHRDYLTIQYQNADRISIPVEQIELLTKYVSADGKEPKINTLNDGRFKKAKQ
                     RVAKQVEDIADDLLKLYAERSQLQGFAFSPDDNMQNDFDNDFAYVETEDQLRSIKEIK
                     QDMEGNRPMDRLLVGDVGFGKTEVAMRAAFKAVNDHKQVVVLVPTTVLAQQHFENFKE
                     RFSNYPVTVDVLSRFRSKKEQTDTLKRLSKGQVDIIIGTHRLLSQDVVFSDLGLIVID
                     EEQRFGVKHKEKLKELKTKVDVLTLTATPIPRTLHMSMLGIRDLSVIETPPTNRYPVQ
                     TYVLETNPGLVREAIIREIDRGGQVFYVYNKVDTIDQKVSELQELVPEASIGFVHGQM
                     SEIQLENTLIDFINGDYDVLVATTIIETGVDISNVNTLFVENADHMGLSTLYQLRGRV
                     GRSNRIAYAYLMYRPDKVLTEISEKRLDAIKGFTELGSGFKIAMRDLSIRGAGNILGA
                     SQSGFIDSVGFEMYSQLLEQAIATKQGKSLIRQKGNAELALQIDAYLPAEYISDERQK
                     IEIYKRIRELETRADYEALQDELIDRFGEYPDQVAYLLEIGLLKAYLDLAFTELVERK
                     GNEISILFEKASLKYFLTQDYFEALSKTQLKARISETNGKMEVVFNIKHKKNYEIIEE
                     LLKFAECFIEIKSRKPVEE"
     gene            8406..8678
                     /locus_tag="ACUBV3_001156"
     CDS             8406..8678
                     /locus_tag="ACUBV3_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HHU3847661.1"
                     /translation="MRLDKYLKVSRIIKRRPVAKEVADKGRVKVNGVLAKSSTDLKLN
                     DQVEIRFGNKLLTVKVLEMKDSTKKEDAIKMYEIINETRIETDEQA"
     gene            8665..9036
                     /locus_tag="ACUBV3_001157"
     CDS             8665..9036
                     /locus_tag="ACUBV3_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HHU3847662.1"
                     /translation="MSKPNVVQLNNQYINDENLKKRYEAEELRRKNRLMGWVLIFVML
                     LFILPTYNLVKSYRTLQERRQEVVKLTKDYQTLTNRTENQKLLAKQLKNPDYVQKYAR
                     AKYYFSKTGEMIYPLPDLLPK"
     gene            9039..9173
                     /locus_tag="ACUBV3_001158"
     CDS             9039..9173
                     /locus_tag="ACUBV3_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_0009 family protein"
                     /protein_id="HHU3847663.1"
                     /translation="MNNLIETIEHFLAYSDDKLEELADKNYQLRQTIVEKEKIESREK
                     "
     gene            9173..10459
                     /locus_tag="ACUBV3_001159"
     CDS             9173..10459
                     /locus_tag="ACUBV3_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HHU3847664.1"
                     /translation="MKKVLTFLLCSLYFVSIPAISTEEPLTLSQNRRYALTQTVVDKE
                     MYFDAIPERPTTKIEISSFQDEALTITGETLVPNTLLSIVSLTINSNGIPVFTLSNGQ
                     FIKASREAIFNDLVSKQQSVSLDYWLKPSFVTYEAPYTNGVSEVKNNLKPYSRVHLVE
                     QAETEHGIYYKTDSGFWISVEDLSVADNRMAKVQEVLLEKYNKDKYGIYIKQLNTQTV
                     AGINIDRSMYSASIAKLATLYASQEQVKLGKLSLDSKFEYKDNVNQFPNSYDPSGSGK
                     LEKKADHKLYTVKELLEATAKESDNVATNMLGYYVNNQYDSMFQTQVDTISGMHWDMK
                     KRQISPQAAGKMMEAIYYQNGDIVNYLSKTDFDNTRIPKNIPVKVAHKIGDAYDYKHD
                     AAIVYAEQPFIMIIFTDKSSYDDITKIADDVYQVLK"
     gene            10461..11735
                     /gene="tilS"
                     /locus_tag="ACUBV3_001160"
     CDS             10461..11735
                     /gene="tilS"
                     /locus_tag="ACUBV3_001160"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HHU3847665.1"
                     /translation="MYNTILKDTLSKGLFTAHQKVLIAVSGGIDSINLLQFLYQYQKE
                     LSISIGIAHINHGQRKESEKEEEYIRQWGQIHDVPVFISYFQGIFSEDRARNHRYNFF
                     SKVMREEGYTALVTAHHADDQAETVFMRILRGSRLRYLSGIKQVSAFANGQLIRPFLP
                     YKKELLPNIFHFEDASNASSDYLRNRIRNVYFPALERENNQLKDSLITLSEETECLFT
                     ALTDLTRSIEVTNCYDFLRQTHSVQEFLLQDYISKFPDLQVSKEQFRVILKLIRTKAN
                     IDYTIKSGYFLHKDYESFHITKIHPKTDSFKVEKRLELHNIQIFSQYLFSYGKFISQA
                     DITIPIYDTSPIILRRRKEGDRIFLGNHTKKIRRLFIDEKITLKEREEAVIGEQNKEL
                     IFVIVAGRTYLRKPSEHDIMKGKLYIENLEKR"
     gene            11740..12282
                     /gene="hpt"
                     /locus_tag="ACUBV3_001161"
     CDS             11740..12282
                     /gene="hpt"
                     /locus_tag="ACUBV3_001161"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012961310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HHU3847666.1"
                     /translation="MLENDIKKVLYSEEDIILKTKELGAKLTADYAGKNPLLVGVLKG
                     SVPFMAELLKHIDTHVEIDFMVVSSYHGGTTSSGEVKILKDVDTNIEGRDVIFIEDII
                     DTGRTLKYLRDMFKYRQANSVKVATLFDKPEGRLVDIDADYVCYDIPNEFIVGFGLDY
                     AENYRNLPYVGVLKEEIYSK"
     gene            12305..14275
                     /gene="ftsH"
                     /locus_tag="ACUBV3_001162"
     CDS             12305..14275
                     /gene="ftsH"
                     /locus_tag="ACUBV3_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HHU3847667.1"
                     /translation="MKNNKNNGFLKNSFIYILLIIAVITTFQYYLKGTSSQNQQISYT
                     KLVKQLKAGEIKSISYQPSGGVVEVSGTYKKAKTIKSANSFTFLGGSVATKVTGFNSV
                     ILPNDSSIKSLVSAAEENNTNIQVKHESSSGTWISYIASFLPLVIMIGFFMMMMNQGG
                     GARGAMSFGKNKARSSSKDEVKVRFSDVAGAEEEKQELIEVVDFLKDPKRYKSLGARI
                     PAGVLLEGPPGTGKTLLAKAVAGEAGVPFFSISGSDFVEMFVGVGASRVRSLFEDAKK
                     AERAIIFIDEIDAVGRRRGAGMGGGNDEREQTLNQLLIEMDGFEGNESIIVIAATNRS
                     DVLDPALLRPGRFDRKVLVGQPDVKGREAILRVHAKNKPLADNVDLKVVAQQTPGFVG
                     ADLENVLNEAALVAARRNKKVIDASDIDEAEDRVIAGPSKKDRTISERERAMVAYHEA
                     GHTIVGLILSNARVVHKVTIVPRGRAGGYMIALPKEDQMLLSKDDMKEQLAGLMGGRV
                     AEEIIFNAQTTGASNDFEQATAMARAMVTEYGMSEKLGPVQYEGNHAMMAGQMSPEKS
                     YSAQTAQLIDDEVRHLLNEARNKAADIINENRDTHKLIAEALLKYETLDAAQIKSIFE
                     TGKMPETENDEDKARALSYDEIKEKMQEEDKK"
BASE COUNT         5036 a         2059 c         2703 g         4486 t
ORIGIN      
        1 caataaataa aaatttcttc ttgcatgctc taacggtaac caaacgagct attagtcata
       61 aaaatgcgat tccaatcctt tcaactgtta aaatagaagt gactagggat gctatcattt
      121 taacggggtc aaatggacaa atttcaattg aaaatactat tcctgcttca aatgaaaatg
      181 caggtttact agtaacgaat ccaggctcta ttttgttaga agctggtttc tttattaata
      241 ttatttcaag tttaccagat gtaactttag aatttacaga gattgaacaa catcaaattg
      301 ttcttactag tggaaaatca gagattactt tgaaaggtaa ggatgtcgat caataccctc
      361 gtctgcagga aatgacaaca gatactccat taacattaga aactaaactg ttaaaatcaa
      421 ttattaatga aactgctttt gctgctagcc aacaagaaag ccgtccaatc ttaacaggtg
      481 ttcatttggt tatcagtcaa aataaatact ttaaggctgt tgcgacagat tcacaccgta
      541 tgagtcaacg cactttccaa ttagagaaat cggctaataa ttttgatttg gttgttccaa
      601 gtaaatccct tcgagaattt tcggctgttt ttacagatga tattgaaact gtagaggttt
      661 tcttctcaga tagtcaaatg ttatttagaa gtgaaaatat cagcttctat acacgtttgc
      721 ttgaaggaaa ctaccctgat actgatcgcc tcctaactaa tcagtttgaa accgaaatta
      781 tctttaatac aaatgcttta cgccatgcta tggaacgtgc ttatttaatt tcgaatgcaa
      841 ctcagaacgg tactgttcgt ttagaaattc aaaatgagac agtctcagct catgtaaact
      901 ctccagaagt tggtaaagtt aatgaggaat tggatactgt tagtcttaaa ggtgatagtt
      961 taaatattag ctttaatcca acttacctaa ttgaatcttt aaaagcagta aaaagcgaaa
     1021 cagttacgat tcgatttatt tctccagtac gtccatttac tttgacacct ggtgaagata
     1081 ctgaagattt catccaatta ataactcctg ttcgtactaa ctaaataaga aaactaccca
     1141 agttattctt gggtagtttt cttattctaa gatatgctat aatgatgagt aatatgactc
     1201 tatatataat agctaacccc catgctggta ataaaaatgc ctccactatt gttggtaaaa
     1261 ttcaggagct ttatcatact gaagatattt ctgtgttcta tacagaacag aaagatgatg
     1321 aaaaaaaaca agtcattaat atactaaggt cttttaaaga aagtgatcat ctaatgatta
     1381 taggaggaga tggtacctta tcaaaagtaa tgacttatct cccccaacat attccgtgcg
     1441 cttattatcc tgttggttcg ggaaatgatt ttgccagagc tttgaaaatt cctaacctca
     1501 aagaaaccct taccgctatt cagacagaaa gattaaagga aattaattgc tttatttatg
     1561 ataaagggct gattttgaat agtttagatt taggttttgc agcctatgtc gtttggaaag
     1621 catctaactc taaaataaaa aatattttga atagatatcg tctaggaaaa ataacttaca
     1681 ttgttatagc tatcaaatca ttattacatt cttctaaagt acaagtatta gtggaaggag
     1741 agacaggaca gcagataaaa ctaaatgacc tttatttttt cgcattagca aataacactt
     1801 attttggagg tggcattact atttggccta aagcaagtgc tttaactgca gagttagata
     1861 tggtttacgc taaagggcat acttttctaa aacgattaag tattttatta tctttggttt
     1921 ttaaaaggca tactacctct aaaagtataa aacaccagac tttcaaagct atgaccgtat
     1981 attttcctaa aaatagtttg attgaaatag atggcgaaat agttgagtta gatcagatta
     2041 gtttaaaatg tcaaaaaagg tatttatata tgtaaggaga aaagatgtat caagttggat
     2101 cacttgttga aatgaaaaaa cctcatgctt gtgtaattaa agaaactggt aaaaaggcta
     2161 atcaatggaa ggtactgaga gtaggagctg atattaaaat tcagtgtact aactgtcagc
     2221 atgtgattat gatgagtcgt tatgattttg agcgaaaatt aaaaaaagta ctgcaaccat
     2281 gaaagtcctt ttaaacaaat aattatagct atatttgcaa ccaatacttt cctaaataat
     2341 gctagtatgc cgttggaaaa ttagctattc taatgttatc gaaagaagaa aggtggtcat
     2401 tgacaatgac gaaagttgca gaacagttat ttatttttcg gtaagtcata tcaaaatggg
     2461 aaaatgggga taccacacca gatttggata atttaatcag gttagctgag atttttgacg
     2521 tgagcttaga tgaacatgtt ttagctaaaa caaatgaagt taaagttgaa cgtatttatg
     2581 aaaataaacc ccttgatcta cgaaaataca ataaaattta ttggtttatt tttcgaaata
     2641 ttattcgatc tctactaatt attttagcta tattaacttt cttataagct ctaggtattc
     2701 cctttgtttc ggagtggata atttaaatga aggtgaaaga gtggcataat gataagcaaa
     2761 tctgaaattt tcttcttatc ataccgcttt ttcttctatt ttttgatata atagtcttga
     2821 ttgaaatttg aatggagact cactaaatgg ctttaacagc aggtattgta ggcttaccaa
     2881 atgttggtaa atcaacttta tttaacgcaa ttacaaaagc aggagcagaa gctgctaatt
     2941 atccttttgc gactattgat cccaatgttg gtatggtaga agtaccagat gagcgtctac
     3001 aaaaattgac agaattgatt acgcctaaaa aaacagttcc gacaaccttt gaatttactg
     3061 atattgcagg tattgttaaa ggtgcttcga agggggaagg tctaggtaat aaatttttag
     3121 ctaatattcg tgaagtggat gctattgttc atgtggtacg tgcgtttgat gatgaaaatg
     3181 ttatgcgtga acaaggtcgt gaggatgctt tcgttgatcc tatagcagat attgatacta
     3241 ttaatcttga attaatctta gctgatttag agtcaatcaa taaacgttat gcgcgtgttg
     3301 aaaaaatggc acgaactcaa aaagataaag aatcagtagc agagttcaat gttttacaaa
     3361 agattaaacc tgttttggaa gatgggaaat cagctcgtac cattgagttt acagaagagg
     3421 aagcaaaagt tgttaaaggt ctttttttat taacaactaa acctgtttta tatgttgcta
     3481 atgttgatga ggacaaagta gctgatcctg atgatatcga ctatgttaat caaattcgtg
     3541 catttgcaga aacagagaat gcagaagtag tcgtgatttc tgctcgagct gaagaagaaa
     3601 tatctgaact tgatgatgaa gataagttag aatttttaga agctattgga ttaacagaat
     3661 ctggtgttga taaattaaca cgagctgctt atcatctttt aggccttgga acttacttta
     3721 ctgctggtga aaaagaggtt cgcgcttgga cgtttaagcg tggtataaaa gctccacaag
     3781 ctgctagtat tatccattca gattttgaaa gaggttttat tcgtgcagta acgatgtcat
     3841 atgatgacct tattcaatat ggatctgaaa aagcagtaaa agaagctggc cggttacgtg
     3901 aagaaggaaa agagtatatt gttcaagatg gcgatatcat ggaattccgt ttcaatgtct
     3961 agtaataaat aatcgatttg ttaactttta ggttaaggat attgttaata tccttaacca
     4021 tttttgcgtt ttaatggaga agataatggt aaaaatgatt gttggattag gaaatccagg
     4081 aagtaaatat aatgatacaa aacataatat tggatttatg gctgttgaca gaattgtaaa
     4141 agaccttgac gtcaacttta cagaagataa aaattttaaa gcagagatag gtagtgattt
     4201 tatcaacggt gaaaaaattt attttatcaa accgactact tttatgaata acagtggtat
     4261 tgctgtaaaa gccctgctta cttactacaa catttctatt aaggatatga ttatcatata
     4321 tgatgattta gacatggaag tcggtaaaat acgttttcgt caaaaaggat ctgctggggg
     4381 acataatggt atcaagtcta ttattgctca tttaggtact caagaatttg atcgtattaa
     4441 agtaggtata ggacgtccga atggacgtat gacagttatt aatcatgttc tagggaaatt
     4501 tgataaaaat gatgaaatta tgatattaaa tacccttgat aaggttgaca atgctgtcaa
     4561 ttattattta cagacgaatg attttcaaaa aacaatgcag aaatacaacg gtttaaaatg
     4621 aatattatag aattatttag ccaaaataaa gtggtgtgga catggcactc tggattagta
     4681 actaatagtc gccaattagt tatggggttt tcaggagcaa gtaaagctat tgccatagca
     4741 tcagcctacg agaagttatc taaaaaaatt atggtagtca ctgccactca aaccgattct
     4801 gataaattat caagtgatat atcaagttta attggagaag ataacgttta tcaatttttt
     4861 gctgatgacg ttcctgcagc agagtttatt ttctcttcat tagataaatc aatttcacga
     4921 ctcagtgccc tgaggtttct aaaagatcca gaaaaaaacg gtgttttgat tactagtatc
     4981 agtggtttaa ggttactatt accaaatccg gaagttttct cgaaaagtca atataagttt
     5041 gaaatcggtc aagaatgtta tttagataaa ttgtgtaaga acctagtcaa tttaggttat
     5101 cagaaagttt ctcaagtatt tagtcctggt gaatttagcc aaagaggaga tattttagat
     5161 atttttgaga tgacacaaga atatccttat cgcttggaat tcttcggaga tgaaatagat
     5221 ggcatcaggc agtttgatat tgacactcaa aagtctctta aacaattaga gtcagtacag
     5281 atttcgccag cagatgatat tattttacaa gatgcagatt tcgagcgtgc aaagaaaaaa
     5341 cttgagggct atctagtaac agctagtgag ggacaacgca cctatttgag cgaggtttta
     5401 tctacaactg agaatcattt taagcactca gatattcgtc gttttctaag tatcttttat
     5461 gaaaaagaat ggggaatatt agactatatt cctgagggaa ctccactttt tgttgatgat
     5521 tttcaaaaaa ttgtagatcg aaatgctaag ttagatttag aaatagctag ccttctgacg
     5581 gaagatttac aacaaggcaa gtctcattct agtttgaact atttttcaga tccttataag
     5641 caattaagac aataccaacc agcaaccttc ttttcaaatt tccacaaagg attaggaaat
     5701 ctgaaatttg ataaacttca tcattttact caatatggaa tgcaagaatt ttttaatcaa
     5761 tttccattgt tggtcgatga aatcaatcgt tataagaaat ctggagcgac agtactgttg
     5821 caagtagatt ctcaaaaagg tttaaatctc cttcaagaaa atttaaaaga atatgggtta
     5881 gatttaatca tttcagataa aaacgatatc gttcaaaagg agtcacaact tattgttggg
     5941 catctttcaa atggttttta ttttgcagat gagaaaattg ttctgattac tgaacgtgag
     6001 atttatcata ggcgtgttaa aaggaaaatt agacgcagta atattagtaa tgcagaacgt
     6061 ctaaaagatt ataatgaact ttcagttggc gattatgttg ttcataatgt tcatggcgta
     6121 ggaaaatttt taggtattga aacaattgag atacaaggta ttcatcgtga ttatctgact
     6181 attcaatatc aaaatgctga tcgtatctct atccccgtag aacaaattga acttctaaca
     6241 aaatatgtct ctgcagatgg caaagagcct aaaattaata cgttaaatga tggtcgcttc
     6301 aaaaaagcaa aacagagagt tgcaaagcaa gtagaagata ttgctgatga tttgttgaaa
     6361 ctttatgcag agcgtagtca attacaagga tttgcatttt ctcctgacga taatatgcaa
     6421 aatgactttg ataatgattt tgcatatgta gagacagagg atcaattgcg gtctataaaa
     6481 gaaattaaac aggatatgga aggtaatcgt ccgatggatc gattattggt tggagacgtt
     6541 ggttttggta agacagaggt tgctatgcgt gcggctttca aagctgtcaa tgaccataaa
     6601 caagttgttg ttttagtccc aacaacagtt ctagcccagc agcattttga aaattttaaa
     6661 gaacgtttta gcaattatcc tgttacagtt gatgtactta gtcgttttcg tagcaaaaaa
     6721 gaacaaacag atacactaaa aagattatct aagggccaag ttgatattat tattggaaca
     6781 catcgacttc tctctcaaga tgttgtattt tcggatttag ggttaatagt tattgatgag
     6841 gaacagcgat ttggtgtaaa gcataaagaa aagttaaaag aattaaaaac aaaagttgat
     6901 gtattaactt tgacagcgac cccaatacct agaacattac atatgtcaat gcttggcatt
     6961 agagacttat cagtcattga aacacctcca actaatcgtt atcctgtgca gacttacgtt
     7021 ttggagacaa atcctggttt agttagagaa gctattattc gtgagataga cagaggagga
     7081 caggtttttt atgtttacaa taaagttgac actattgatc agaaagtttc agaacttcag
     7141 gagttagtac cagaagctag tattggtttt gttcatggtc aaatgtctga aattcaatta
     7201 gaaaatacat taatagactt tatcaatgga gattacgatg ttttagttgc aacgacaata
     7261 attgagacag gagtcgatat ttcaaatgtt aataccctct ttgttgaaaa tgcagatcat
     7321 atgggcttgt caacccttta tcaattaaga ggccgtgtgg gtagaagtaa tcgcattgct
     7381 tatgcttacc ttatgtacag acctgataaa gttttaacag aaatttctga gaaaagacta
     7441 gatgcaatca aagggtttac agaattggga tctggtttta aaatagcgat gcgtgattta
     7501 tctatacgtg gtgcaggaaa tattttagga gcttcacaaa gtggttttat tgattcggtt
     7561 ggattcgaga tgtattcaca gcttctagag caagcgattg ccaccaaaca agggaaatca
     7621 ttaattcgtc aaaaaggtaa tgctgaatta gccttacaaa ttgacgccta tttaccagca
     7681 gaatatatat cagatgagcg ccaaaaaata gaaatatata agcgtattcg tgaattagaa
     7741 actagggctg attatgaagc cttacaagat gagttaatag atcgttttgg agaatatcct
     7801 gatcaagttg catacctttt agaaatagga cttttaaaag cttatctgga ccttgccttt
     7861 actgaattag tagaaagaaa aggtaatgaa ataagcatcc tatttgaaaa agcatctttg
     7921 aagtattttt taactcaaga ttattttgaa gcactatcaa aaactcaact taaggctaga
     7981 attagcgaaa caaatggtaa gatggaagtt gtttttaata tcaaacataa gaaaaattat
     8041 gaaattattg aagaacttct aaaatttgcc gaatgtttta tcgagattaa gtcacgtaaa
     8101 ccagtagaag aataactaag tgaaaagttg caaggaaatt tggatactct tttagaaaat
     8161 gtttggttca agaagaatag tacgagcaac attatgtttc acgtcaaaca tctcaacttt
     8221 tgaaagacat aaatattttt agaggttatt aaaatagaag ttttttactt gatatttgag
     8281 agaattaaga gagctattag ctctcttttt gttacattat attgacaaat attatcagtt
     8341 cagatatttt tcaaaaaaaa tgctaaaata gtagagttaa tttagataga aggatgtgat
     8401 catcagtgag attagacaag tatctaaaag tgtcgcgtat cattaagcgt cgtcccgttg
     8461 ctaaagaagt tgcagacaaa ggacgtgtca aggtgaatgg cgtattagca aagtcatcaa
     8521 ctgacctaaa gcttaatgac caagttgaaa ttcgttttgg taataaattg ttgaccgtta
     8581 aagtattaga aatgaaagat agcactaaga aagaagatgc tatcaaaatg tatgaaatta
     8641 ttaatgaaac aaggatagaa acagatgagc aagcctaatg ttgttcagtt aaataatcaa
     8701 tatattaacg atgagaatct aaaaaaacgt tacgaagctg aggagttacg ccgaaaaaat
     8761 cgtttaatgg gttgggttct tatttttgtc atgcttttat ttattttacc cacttataat
     8821 ttagttaaga gttacagaac tttacaagaa cgtcgtcaag aagttgtaaa attaacgaaa
     8881 gactatcaga cattaactaa tagaactgag aaccagaagt tgctagcaaa acaactaaaa
     8941 aatccagatt acgttcaaaa atatgctcga gctaagtatt atttctctaa gaccggcgaa
     9001 atgatttacc cattaccaga ccttttacca aaataattat gaataattta attgaaacaa
     9061 tagaacattt tctagcctac tcggatgaca aactagaaga attagctgat aagaactatc
     9121 aacttaggca aacaattgta gaaaaagaaa agattgagag cagagaaaag taatgaagaa
     9181 agttttaacc ttcttattat gtagcttata ttttgtatca atcccagcta taagtaccga
     9241 ggagccatta acgctttctc aaaatagacg atacgcctta actcagacag ttgtggataa
     9301 ggaaatgtat tttgatgcta ttccagagag accaactaca aaaattgaaa taagcagttt
     9361 tcaagatgaa gctttaacca taacaggaga gactttagtt ccaaatactt tattgtcgat
     9421 tgtttctctt acaattaatt caaatggaat tccagtattt actttgtcca atggacagtt
     9481 cataaaggct agtcgtgaag ctatttttaa tgatttagtt tctaagcagc aatctgtctc
     9541 attggattat tggttaaaac cttcttttgt gacttatgaa gcaccttaca caaatggtgt
     9601 tagtgaagtt aaaaataatt taaaacctta ttccagagtg catttagtag aacaagcaga
     9661 aacagaacat ggtatttatt ataaaacgga cagtggtttt tggatatcag tagaagatct
     9721 atcggttgct gataatcgta tggctaaggt tcaagaagta ttattggaaa aatataataa
     9781 agataaatat ggtatataca tcaaacaatt aaatacacag acagtagctg gtattaatat
     9841 tgataggtca atgtactctg caagtattgc aaaattagca acactttatg ctagccaaga
     9901 gcaagtaaaa ttaggaaaac tgtcattgga cagcaaattt gaatacaaag ataatgttaa
     9961 tcaatttcct aatagctatg atccaagtgg tagtgggaaa ttagagaaaa aagctgatca
    10021 taaattatat accgttaagg agttacttga agcaactgcc aaggaatcag ataatgttgc
    10081 aacaaatatg ctaggttatt atgtcaataa tcaatatgat tcaatgtttc aaacacaagt
    10141 agacacaatt tctggtatgc actgggatat gaaaaaacgc cagatttctc cacaagctgc
    10201 gggtaaaatg atggaagcaa tttattatca aaatggagat attgttaatt acctatcaaa
    10261 gactgatttt gataatacaa gaatccctaa aaatatacca gttaaagtag cacataaaat
    10321 tggagatgct tatgactaca aacatgatgc ggctattgtt tatgcagaac aaccttttat
    10381 tatgattatc tttacggata aatcatctta cgatgatatc actaagattg ctgacgacgt
    10441 ttaccaggtt ttaaaataat atgtataaca ctattttgaa agatacctta tccaaagggt
    10501 tatttaccgc tcatcagaaa gttctaattg ctgtttctgg tggcattgat tcaataaatt
    10561 tgttgcaatt tttataccag tatcaaaaag agttatcgat tagtattggt attgcacaca
    10621 taaatcatgg acagcgaaaa gaatcagaaa aagaagaaga atatatcaga cagtggggac
    10681 agatacatga tgtccctgtc tttatctctt attttcaagg aatattttca gaagatagag
    10741 cacgtaacca tcgctacaat tttttttcaa aagttatgcg tgaagaaggt tacacggctt
    10801 tagtaacagc tcaccatgct gatgatcaag ctgaaactgt atttatgaga attttaagag
    10861 gaagcaggct acgctattta tcagggatta agcaagtgag tgcttttgca aacggtcaat
    10921 tgattagacc atttcttcca tataaaaagg agttattacc aaatattttt cattttgaag
    10981 atgctagcaa cgcttcgtct gattatttaa gaaatcgaat aagaaacgtc tactttcctg
    11041 ctttggaaag agaaaataat caattgaaag attctctcat aactctctct gaggaaacag
    11101 agtgtttatt cacggcttta acagacctaa caaggagcat cgaagtaaca aactgctatg
    11161 attttttaag gcaaactcat tctgtccaag aatttttatt gcaagattac attagcaaat
    11221 ttccagattt acaagtgagt aaagaacagt tcagagtaat attaaaattg attagaacta
    11281 aagccaatat tgactatact attaaatcgg gctattttct tcataaggat tacgagagtt
    11341 ttcatattac aaaaatccat cctaagacgg atagttttaa ggttgaaaaa agattagaat
    11401 tgcataatat acaaatattc tcacaatatt tattttccta tggaaaattc atttctcaag
    11461 cagacattac tattcctatt tatgatactt ctccgattat tttaagaaga agaaaagaag
    11521 gggataggat atttttagga aatcacacta aaaaaattcg ccgtctattt attgatgaga
    11581 aaataactct aaaagagaga gaagaagctg ttattgggga gcaaaataaa gagttgatat
    11641 ttgtgattgt tgcaggtaga acatatttga gaaaaccttc tgaacatgat ataatgaagg
    11701 gcaaacttta tattgaaaat ttagaaaaaa ggtgacatca tgcttgaaaa tgatataaaa
    11761 aaagttttat attcagaaga agatattatt ctgaaaacga aagaattagg agctaaatta
    11821 actgcagatt atgcaggtaa gaatccatta ttagtaggtg tattaaaggg gtcagtgcct
    11881 tttatggctg agctattaaa gcatattgat acacatgttg aaattgattt tatggttgtt
    11941 tcaagctatc atggtggtac aactagtagt ggagaagtta aaattttaaa agatgttgat
    12001 actaacattg aaggaagaga tgtaatcttt attgaagaca tcattgatac tggacgtaca
    12061 ttaaaatacc ttcgagatat gtttaagtat cgccaagcta actctgtcaa agttgcaacc
    12121 ctttttgata agccagaagg gcgattggta gatattgatg ctgattacgt ttgttacgat
    12181 atcccaaatg aatttattgt gggattcggt ttagactatg ctgaaaacta tcgtaactta
    12241 ccttatgttg gtgtgttaaa agaagaaatt tactcaaaat agaaaaaagg tactctctta
    12301 aactatgaaa aataataaaa ataatggttt tctgaaaaat tcctttattt acatattatt
    12361 gattattgcg gttattacaa cctttcaata ctatttaaaa ggaactagct ctcaaaacca
    12421 gcaaatttct tatacaaaac ttgttaaaca attaaaagct ggagaaatta agtctatctc
    12481 ataccagcca agtggtggtg ttgttgaagt tagtgggaca tataagaaag ccaaaacaat
    12541 taaatcagct aatagtttta cttttttagg tggtagtgtt gctactaaag ttacaggatt
    12601 taattctgtt atactaccaa atgactcttc aataaaatct cttgttagcg ctgctgaaga
    12661 aaataacact aatattcagg tcaaacatga aagttcaagt gggacatgga tttcttatat
    12721 tgccagtttt ttacctttag ttatcatgat tggtttcttc atgatgatga tgaaccaagg
    12781 cggtggcgct cgtggtgcca tgagctttgg taaaaataaa gcaagatcat ctagtaaaga
    12841 tgaggttaaa gttcgatttt cggatgttgc tggagcagag gaagaaaagc aagaattaat
    12901 agaagtcgtt gacttcctta aagatcctaa acgatataaa tctctaggtg cccgaattcc
    12961 agcaggggtt ctattagaag gacctccagg gacaggtaaa accctattag caaaagctgt
    13021 tgccggtgaa gcaggggttc cattctttag tatttccggt tcagattttg ttgaaatgtt
    13081 tgtcggtgtt ggagctagtc gagtacgctc tttgtttgaa gatgctaaaa aagctgaacg
    13141 tgctattatt ttcattgatg aaattgatgc tgttggacgt cgacgtggag ctggtatggg
    13201 tggcggaaac gatgaacgtg aacaaacgct caaccaactt ttaattgaaa tggatggttt
    13261 tgaaggaaac gaaagtatta tcgttatcgc tgctaccaac cgtagtgatg tgttagatcc
    13321 cgcattactg cgtccaggac gttttgatag aaaagttcta gttggacaac ctgatgtaaa
    13381 aggtcgtgaa gcaattctga gagtacatgc taaaaataaa ccattagcag ataatgttga
    13441 cttaaaagta gtagctcaac aaaccccagg atttgttggt gctgatttgg agaatgttct
    13501 caatgaagct gctttagttg ctgcgcgacg aaataaaaaa gttattgatg cttcagatat
    13561 tgatgaagca gaagaccgtg taattgctgg tccttctaaa aaagatcgta ctatttctga
    13621 aagagaaaga gctatggttg cttaccatga agctgggcac acaattgtcg gtttgattct
    13681 ttcaaatgcg cgagttgttc ataaagttac tatcgtacca cgtggtcgtg caggtggata
    13741 tatgattgct ttacctaaag aagatcaaat gcttctttca aaagatgata tgaaagagca
    13801 attagcaggt cttatgggag gacgtgtagc ggaagaaatt attttcaacg ctcagacaac
    13861 aggtgcctca aatgacttcg aacaagccac tgctatggcc cgcgctatgg taactgaata
    13921 tggtatgagc gagaaacttg gaccagttca atacgaaggc aaccacgcaa tgatggctgg
    13981 tcaaatgtca cctgaaaaat catattctgc tcaaactgca caacttattg atgatgaagt
    14041 tcgtcacctt cttaatgaag cacgtaataa ggcagctgat attatcaacg aaaaccgtga
    14101 tactcataaa ttaattgcag aagccctctt aaaatatgag actcttgatg ctgctcagat
    14161 taaatcgatt tttgagacag gtaaaatgcc tgagacagaa aatgatgaag ataaagcaag
    14221 agcgttatca tacgatgaaa tcaaagaaaa aatgcaagaa gaagataaaa aataagtcat
    14281 cata
//
LOCUS       DBJYSH010000051        14273 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.051, whole genome shotgun sequence.
ACCESSION   DBJYSH010000051 DBJYSH010000000
VERSION     DBJYSH010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 14273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14273
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.051"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            21..722
                     /locus_tag="ACUBV3_001163"
     CDS             21..722
                     /locus_tag="ACUBV3_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001263956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HHU3847668.1"
                     /translation="MRVITVKNDIEGGKIAFTLLEEKMKTGAQTLGLATGSSPITFYE
                     EIVKSNLDFSNMVSINLDEYVGIAASNDQSYSYFMHKHLFDAKPFKENNLPNGLAKDL
                     KKEIKRYDAVINANPIDFQILGIGRNGHIGFNEPGTPFDITTHVVDLAPSTIEANSRF
                     FNSIDDVPKQALSMGIGSIMKSKTIVLVAYGIEKAEAIASMIKGPITEDMPASILQKH
                     DDVVIIVDEAAASKL"
     gene            complement(794..1750)
                     /locus_tag="ACUBV3_001164"
     CDS             complement(794..1750)
                     /locus_tag="ACUBV3_001164"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004364 - glutathione transferase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HHU3847669.1"
                     /translation="MGLLVDGKWVDQWYDTASTGGKFVRTVTQFRHWVTKDGSAGPSG
                     DAGFKAESGRYHLYVSLACPWASRVLIMRKLKNLESHISISIVNPLMLENGWTFQEYK
                     GVIPDMINQSQYLYQIYQASQSDYTGRVTVPVLWDKKCHTIVNNESSEIMRMLNTAFN
                     HITGNTDDYYPDSLQGQIDEMNNFIYPKINNGVYKAGFATSQNVYQKEVETLFTALDQ
                     LEKHLSDNHYLVGEQFTEADIRLFTTLVRFDTVYYGHFKCNLKALHDYPHLWHYTKRI
                     YNLPGIAETINFDHIKKHYYGSHKTINPTGIIPAGPNLDWTI"
     gene            1846..2565
                     /locus_tag="ACUBV3_001165"
     CDS             1846..2565
                     /locus_tag="ACUBV3_001165"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HHU3847670.1"
                     /translation="MRLDKLLGQAGFGSRNQVKKLIRSRQVSVDGQIVTKDNVIVDSG
                     LQSIFVGKERVCLKESSYYLLYKPSGVVSAVRDSEHKTVIDLISEKDKVEGLYPIGRL
                     DRDTEGLLIITNNGPLGYRMLHPKHHVAKTYYVEVNGFLERDAITFFEEGVVFDDGTK
                     CKPAELTIDTANNDKSTARITITEGKFHQVKKMFLAYGVKVIYLRRISFGDLRLDMNL
                     KPGQYRRLRDSEAAILKRYLD"
     gene            2638..3789
                     /locus_tag="ACUBV3_001166"
     CDS             2638..3789
                     /locus_tag="ACUBV3_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HHU3847671.1"
                     /translation="MNSKLSLKLTILSISIFLMSHLAIAPAIPKLYAFYHTQNPHIGL
                     ASVESLVTIPAMMITIFVILSNFVVTKLGKKNTVLLGLCLILMSGFISFFTSNFSLAM
                     ASRLLLGIGIGLYNSLSISIITDLYEADERASMIGLRTASLNIGKALTTFIVGLVLAI
                     GVNYIYLVYLLVIPVFFFFWKNVPEVENQTHTLKASTTFDTKAALLMLITFLVGIAYI
                     GATVKIPTLLVTKYHYATSFSSNMLTLLAFSGILVGSVFGKLVKAFQEKTLLIMILAM
                     GIGNVLFALANNQIIFIVASILIGASFVGTMSSVFFYISKNYAKEHNNFITSLALTAG
                     NIGVILTPLILTKLPSQLHLEPFITPFLITSGLMVINVFVYLVLMSKNK"
     gene            3841..4788
                     /locus_tag="ACUBV3_001167"
     CDS             3841..4788
                     /locus_tag="ACUBV3_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000903651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HHU3847672.1"
                     /translation="MLIAKDKQGNLINLLESHPGKGQYFCPTCCSAVRLKAGRIMRRH
                     FAHISLKNCQFHHENESNEHLQLKAKLYMSLSRENETMLEHHLPEINQIADLFVNETL
                     ALEVQCSRLSEQRLRERTKAYLQADFQVRWLLGEKLWLKHRLTNLHKQFLQFSQSIGF
                     HIWELDLRLEVLRLKYLIYEDLRGHVYYLSKTCPLSGDVLAFLKWPYQSKNLNFYKVK
                     QDRNIRDYVRQQLRYGNQFWLRKQEKAYLSGQNLLTQELMMFFPQIQPPRVDTDFCQI
                     TNSLTSFYQNFTNYYQKNKNNLDQTLYPPVFYDKIVTKN"
     gene            4804..6609
                     /gene="pepF"
                     /locus_tag="ACUBV3_001168"
     CDS             4804..6609
                     /gene="pepF"
                     /locus_tag="ACUBV3_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HHU3847673.1"
                     /translation="MSDNRSHIEEKYQWDLTTVFATDELWETEVVELTQAIDDAKGFS
                     GHLLDSSQSLLEITEVELELSRRLEKVYVYASMKNDQDTTVAKYQEFQAKATALYAKF
                     SETFSFYEPELLQLSESDYQSFLLEMPDLQKYDHFFEKIFANKPHVLSQNEEELLAGA
                     SEIFGAAGETFEILDNADMVFPVVKNAKGEEVELTHGNFISLMESSDRTVRKEAYQAM
                     YSTYEQFQHTYAKTLQTNVKSQNFKARVHHYQSARQSALSANFIPEEVYETLIKTVNH
                     HLPLLHRYMKLRQKVLGLDDLKMYDVYTPLSQMDMSFTYDEALKKSEKVLAIFGEAYS
                     ERVHRAFTERWIDVHVNKGKRSGAYSGGSYDTNAFMLLNWQDTLDNLYTLVHETGHSL
                     HSTFTRENQPYVYGDYSIFLAEIASTTNENILTETLLKEVKDDKNRFAILNHYLDGFK
                     GTIFRQTQFAEFEHAIHVADQEGQVLTSEYLNNLYAELNEKYYGLTKEDNHFIQYEWA
                     RIPHFYYNYYVFQYATGFAAANYLAERIVNGNPEDKEAYLNYLKAGNSDYPLNVIAKA
                     GVDMTSADYLDAAFRVFEERLVELENLVAKGVHND"
     gene            6804..7430
                     /locus_tag="ACUBV3_001169"
     CDS             6804..7430
                     /locus_tag="ACUBV3_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HHU3847674.1"
                     /translation="MKKLTFIWDLDGTLIDSYVPIMEALEETYRHFGLIFDKELIHEY
                     ILQESVGQLLVNLSEEEQIPHEKLKAYFTKEQESRDSKIHLMPYAKEILEWTKEQDIP
                     NFMYTHKGASTHSVLETLQISHYFDEILTGVSGFERKPHPQGINYLVKRYSLDKSMTY
                     YIGDRPLDLEVAQNAGIKSINLRLENSKENYNISSLKDIISLDFTRLD"
     gene            7504..8211
                     /locus_tag="ACUBV3_001170"
     CDS             7504..8211
                     /locus_tag="ACUBV3_001170"
                     /EC_number="2.1.-.-"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HHU3847675.1"
                     /translation="MVQSYSKNANHNMRRPVVKEEIVQYMRQHQKQNNGCLAELEAFA
                     KQENIPIIPHETATYFRFLMQTLQPKHILEIGTAIGFSALLMAENAPEAKITTIDRNE
                     EMIALAKENFAKYDNHNQITLLEGDAVDVLQTLDKSYDFVFMDSAKSKYIVFLPQVLK
                     HLDVGGVVVLDDIFQGGDIAKPIDEVRRGQRTIYRGLQRLFDSTLQHPDLTATLVPLG
                     DGLLMIRKNADHIVLED"
     gene            8272..9201
                     /gene="prsA"
                     /locus_tag="ACUBV3_001171"
     CDS             8272..9201
                     /gene="prsA"
                     /locus_tag="ACUBV3_001171"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PrsA"
                     /protein_id="HHU3847676.1"
                     /translation="MKTRSKLAAGFLTLMSVATLAACSGKTSNGTNVVTMKGDTITVS
                     DFYDQVKTSKAAQQSMLTLILSRVFDTQYGDKVSDKKVSEAYNKTAKGYGNSFSSALS
                     QAGLTPEGYKQQIRTTMLVEYAVKEAAKKELTEANYKEAYKNYTPETSVQVIKLDAED
                     KAKSVLKDVKADGADFAKIAKEKTTATDKKVEYKFDSAGTSLPKEVMSAAFKLDKNGV
                     SDVVSTVDSTTYKTSYYIIKVTDKTEKKSDWKSYKNRLKEVILKDKTSDRSFQNKVIS
                     KALEKANVKIKDKAFAGILSQYATTSGSSSLKK"
     gene            9443..9928
                     /locus_tag="ACUBV3_001172"
     CDS             9443..9928
                     /locus_tag="ACUBV3_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related/scramblase family protein"
                     /protein_id="HHU3847677.1"
                     /translation="MATFQIKEKMFSLGGKFTITDLTGLPCYHVEGSLFPLPKTFKVF
                     DEEEHLISQIEKKVLSFLPKFNVTLANGNHFTIKKDFSFLKPHYTIEDLDMEVKGNFW
                     DMDFQLLKDNQVIANISQQWFRMTSTYQVEVYSETYNDLTISLVIAIDYVKELEKNAS
                     N"
     gene            9944..12562
                     /gene="alaS"
                     /locus_tag="ACUBV3_001173"
     CDS             9944..12562
                     /gene="alaS"
                     /locus_tag="ACUBV3_001173"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HHU3847678.1"
                     /translation="MKELSSAQIRQMWLDFWKSKGHSVEPSANLVPVNDPTLLWINSG
                     VATLKKYFDGSVIPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFRD
                     EAIEWGFELLTSPEWFDFPKDKLYMTYYPDDKDSYNRWIACGVEPSHLVPIEDNFWEI
                     GAGPSGPDTEIFFDRGEDFDPENIGLRLLAEDIENDRYIEIWNIVLSQFNADPAVPRS
                     EYKELPNKNIDTGAGLERLAAVMQGAKTNFETDLFMPIIREVEKLSGKTYDPDGDNMS
                     FKVIADHIRALSFAIGDGALPGNEGRGYVLRRLLRRAVMHGRRLGINETFLYKLVPTV
                     GQIMESYYPEVLEKRDFIEKIVKREEETFARTIDAGSGHLDSLLAQLKAEGKDTLEGK
                     DIFKLYDTYGFPVELTEELAEDAGYKIDHEGFKSAMKEQQDRARAAVVKGGSMGMQNE
                     TLAGIVEESRFEYDTYSLESSLSVIIADNERTEAVSEGQALLVFAQTPFYAEMGGQVA
                     DTGRIKNDKGDTVAEVVDVQKAPNGQPLHTVNVLASLSVGTNYTLEINKERRLAVEKN
                     HTATHLLHAALHNVIGEHATQAGSLNEEEFLRFDFTHFEAVSNEELRHIEQEVNEQIW
                     NALTITTTETDVETAKEMGAMALFGEKYGKVVRVVQIGNYSVELCGGTHLNNSSEIGL
                     FKIVKEEGIGSGTRRIIAVTGRQAFEAYRNQEDALKEIAATVKAPQLKDATAKVQALS
                     DSLRDLQKENVELKEKAAAAAAGDVFKDIQEAKGVRFIASQVDVADAGALRTFADNWK
                     QKDYSDVLVLVAAIGEKVNVLVASKTKDVHAGNMIKGLAPIVAGRGGGKPDMAMAGGS
                     DASKIAELLAAVAENL"
     gene            12644..13360
                     /locus_tag="ACUBV3_001174"
     CDS             12644..13360
                     /locus_tag="ACUBV3_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000232920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HHU3847679.1"
                     /translation="MVGLGTVINVILIIVGGFVGLFLKNFLKESLQKSLMQAMGVAVL
                     FISISGVLEKMMLVEKSHLISNHTNMMIITLALGTVLGELLSLDSYIDRFGDYLKQKT
                     GSGNDIKFVEAFVTSTCTVCIGAMAVVGSIQDGIAADHSILFAKGMLDMIIIAIMTVS
                     LGKGALFSALPVALLQGSLTIVAFFMGSLLNPSSLDYLNLVGNMLIFCVGVNLLFNLN
                     IKVINMLPAIILAILWGSFI"
     gene            13448..14266
                     /locus_tag="ACUBV3_001175"
     CDS             13448..14266
                     /locus_tag="ACUBV3_001175"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HHU3847680.1"
                     /translation="MNLLFSIDDMYVDHFKVMLYSLVRQTKNRKLEIYVLQKTLLKRH
                     TELIQYTQNLEVGYHPIIVGTEVFAQAPTTDRYPDTIYYRLLAHKFLPETLDRILYLD
                     ADMLCLNDFSSLYDMELGDQLYAAASHNTDGKFLDYVNKLRLKNVELESSYFNTGVLL
                     MNLPAIRKVVHQQTILDYIMQNRGRLILPDQDILNGLYANLVKPIPDEIYNYDARYSL
                     IYQLKSRNEWDLEWVINHTVFLHFAGRDKPWKKDYRGRYSGLYKFMAKEALTKN"
BASE COUNT         4759 a         2292 c         2772 g         4450 t
ORIGIN      
        1 caaataaaag gagagtaaac atgagagtta ttacagttaa gaacgacatt gaggggggaa
       61 aaattgcatt tactctttta gaagagaaaa tgaaaactgg tgctcaaact ttaggattgg
      121 caacaggaag tagcccaatc accttttacg aagagattgt aaaaagtaac cttgattttt
      181 caaatatggt ttctattaac cttgatgaat atgttggaat tgccgcaagt aatgatcaaa
      241 gttactctta ttttatgcat aaacatcttt ttgatgctaa accttttaag gaaaataacc
      301 ttccaaacgg cttggctaaa gatttgaaaa aagaaataaa acgatacgat gctgtcataa
      361 atgctaatcc aattgatttt caaattcttg gaattggtcg taacggtcat attggtttta
      421 atgaaccagg tacacctttt gatattacaa cacatgtggt tgacttagct ccaagtacaa
      481 ttgaagctaa tagtcgtttc tttaatagta ttgatgatgt accaaagcaa gccttatcaa
      541 tgggtatagg ttctattatg aaatcaaaaa caatcgtatt agtggcgtat ggtattgaaa
      601 aagcagaagc gattgcaagt atgattaaag gacctatcac agaagatatg ccagcaagta
      661 tcctccaaaa acatgatgat gttgtgatta ttgtggatga agcagcggca tcaaagttgt
      721 aaagtaaata ataaaaaagt aaggatgaat ctcaatgaga aaaaattcat tcttactttt
      781 ttattatgct ttattaaatt gtccagtcta agttaggtcc agctggaata ataccggtag
      841 ggttaatggt tttatggcta ccgtaatagt gttttttgat atgatcaaaa ttaattgtct
      901 cagcaattcc aggtaggttg taaatacgtt ttgtataatg ccataaatgt gggtaatcat
      961 gtagcgcttt taaattacat ttaaaatgac cataataaac ggtgtcaaag cgaactaaag
     1021 ttgtgaaaag gcgaatatca gcttcggtaa attgttctcc aacaagataa tggttatcag
     1081 ataagtgttt ttctaactga tctaaagctg tgaataaggt ttcaacttcc ttttgataaa
     1141 cgttctgaga agtagcaaaa ccagctttat agacaccatt atttattttt ggataaataa
     1201 aattattcat ttcgtcaatt tgtccttgta aggaatcggg atagtaatcg tcagtatttc
     1261 cagtaatatg attaaatgca gtatttaaca tgcgcataat ttcagatgat tcattgttaa
     1321 cgattgtatg gcatttttta tcccaaagaa cagggactgt cacgcgtcct gtataatcac
     1381 tttgacttgc ttgatagatt tggtaaaggt attggctttg gttaatcata tcaggaataa
     1441 cgcctttata ttcttgaaaa gtccaaccat tctccaacat taaagggtta acaatggaga
     1501 tagagatatg agattctaga tttttaagct ttctcatgat taatacacgt gatgcccaag
     1561 ggcaggctag tgaaacgtag agatgatatc gtccagattc tgctttaaaa cctgcatccc
     1621 cactaggccc tgctgaacca tctttagtaa cccaatgacg gaattgtgta acggttcgga
     1681 cgaatttacc accagttgat gcagtatcat accattgatc aacccatttg ccatctacta
     1741 aaagtcccat gataatctcc tttgtttttc tattactaat tagtagtata aagattttaa
     1801 aaggaattag caaatgttta acttacagta tggtaaacta agggtatgcg tttagataaa
     1861 ttattaggtc aggcaggatt tgggtctaga aatcaggtta agaaattaat tcgttctagg
     1921 caagtttctg tagatggtca aattgttaca aaggataatg ttattgtaga tagtggtcta
     1981 caatcgattt ttgtaggaaa agagagagtc tgcctaaaag aaagttctta ttaccttctt
     2041 tataagccat caggagttgt ttcagcagtt agagattcag aacataaaac agtcatagac
     2101 ttaattagcg aaaaagataa ggtggaggga ttgtatccga ttggtcgtct agatcgtgat
     2161 acggaagggc ttttaataat cacgaataat ggtccactgg gttaccgaat gttacatcca
     2221 aaacaccacg ttgcaaaaac gtattatgtt gaagtaaatg gtttcttgga aagagatgct
     2281 attacctttt ttgaagaagg agttgtattt gacgatggaa caaagtgtaa accagctgaa
     2341 ttaacgattg atacggctaa taatgacaaa agtaccgcaa gaataactat cacagaaggg
     2401 aaatttcacc aagtaaaaaa gatgtttctt gcatatggcg ttaaagttat atacttgagg
     2461 cgtatttctt ttggtgactt gaggcttgat atgaatttga aaccaggtca gtatcgtaga
     2521 ttgagagatt cagaggcagc tatcttaaaa aggtatttag attagtgaaa cgactcactt
     2581 gaccttgctt agtattcttc atatacttat aagtgattat tttaaaggag aaaactattg
     2641 aattcaaaac tttcattgaa attgactatt ttatcgattt ctatcttttt gatgtctcac
     2701 ttagcaattg ctcctgcaat tcctaaattg tatgcctttt accatactca aaaccctcat
     2761 attggactag cttcagtaga gagtttagtt actattccag ctatgatgat tactattttt
     2821 gttattttga gtaattttgt agtcacaaaa cttggcaaaa aaaacacggt cttacttggt
     2881 ttatgtttaa tcttaatgtc tgggtttatc tcatttttta caagtaattt ttcacttgcg
     2941 atggctagtc gactactttt gggtattggt attggtctct ataattcgct atcaattagt
     3001 atcataacgg atttatatga ggctgatgag agagccagta tgattggtct tagaacagca
     3061 tcacttaata tcggtaaagc cttaactaca tttattgttg gattggtttt agctattggt
     3121 gtgaattata tttatttggt ttaccttctg gttattcctg ttttcttttt cttttggaaa
     3181 aatgttccag aggttgaaaa tcaaacacat acccttaaag ctagtacaac atttgatact
     3241 aaagcggctt tgttgatgct catcacgttc ttagtaggta ttgcttatat aggagcaact
     3301 gtcaaaattc caaccttact agtgacaaaa tatcactacg cgacgagctt ctctagtaat
     3361 atgttaactc tccttgcttt tagtggtatt ttagttggat ctgtatttgg aaaattagtc
     3421 aaagcattcc aagagaagac gctattaatc atgattctag ctatgggaat aggtaatgtg
     3481 ctatttgctt tagctaataa tcagatcatt tttatcgtag ctagtatttt aataggagct
     3541 agttttgttg gcaccatgtc ttctgtattc ttttatattt caaaaaacta tgcaaaagaa
     3601 cataataact ttatcacaag ccttgcctta acagctggta atataggagt tattttgaca
     3661 ccgcttattt taacgaaact tccaagccag ctacatctgg aaccttttat aacaccattt
     3721 ttaataacaa gtgggctgat ggtgattaat gtctttgttt acctagtttt aatgtcaaaa
     3781 aataaataaa aagttatccg aaaggatagc ttttttcgaa tagtaaaagt aggagaaaaa
     3841 atgttaattg ctaaagacaa acaaggaaat ttaattaatc tactcgagtc tcatccggga
     3901 aagggacagt atttttgtcc aacttgttgt tcagctgtaa ggttaaaagc aggtcgaatc
     3961 atgagaagac actttgcaca tattagtcta aaaaattgtc agtttcatca tgaaaatgag
     4021 tctaatgagc acttgcaatt aaaagcaaag ttatatatga gtttaagtag agagaatgaa
     4081 actatgctcg agcatcattt gccagaaatt aatcagattg cagatttatt tgtgaatgag
     4141 acattggctt tagaagttca gtgtagccga ctatcagagc agcgactcag ggagcgaaca
     4201 aaagcatatc tacaggcaga ctttcaagtg agatggttac taggggaaaa actctggtta
     4261 aagcatcgtt taacaaatct acataaacaa tttttacaat tttctcaaag tatcggattt
     4321 catatttggg agttagattt gaggttagaa gtactgagac taaaatattt gatttacgaa
     4381 gatttacgcg gacatgtcta ttacttaagt aaaacttgcc ctcttagtgg agacgttcta
     4441 gcatttttga agtggccata ccaatcaaaa aatctaaatt tttataaggt taaacaagac
     4501 agaaatatca gagattatgt gcgtcagcag ttacgctatg gcaatcaatt ttggttacga
     4561 aaacaagaaa aggcctacct ttctggtcag aatctattaa cccaagagct aatgatgttt
     4621 tttcctcaaa tacagccacc acgtgtagat actgattttt gtcaaataac aaattctctg
     4681 acatcgtttt atcagaattt tacaaattat tatcaaaaaa ataagaataa cttggaccaa
     4741 acactttatc cccctgtatt ttatgataaa atagtcacta agaattaaac tggaggatac
     4801 aaaatgtcag acaatcgctc acatattgaa gaaaaatacc aatgggattt aactactgtc
     4861 tttgcgactg atgagttatg ggaaactgaa gtggtagagc ttactcaagc catcgatgac
     4921 gcaaagggtt tttcaggtca tctgttggac tcaagccaat ctttattgga gattacggag
     4981 gttgaactag agttatctcg ccgtttagaa aaagtttatg tctatgcttc aatgaaaaat
     5041 gaccaagaca ccactgttgc gaaatatcaa gaatttcaag cgaaagcgac agcgctctat
     5101 gcaaaattta gtgaaacatt ttcgttttac gagccagaac tattgcaact ttcagagtct
     5161 gattaccagt cattcctttt agaaatgcct gacttgcaaa aatatgatca tttctttgag
     5221 aaaatatttg caaataaacc tcatgtctta tctcaaaacg aagaagaatt gttagctggt
     5281 gctagtgaga tttttggagc agctggtgag acatttgaaa tccttgataa tgcagatatg
     5341 gtcttcccag ttgtaaaaaa tgcaaaaggt gaggaagttg agcttacaca tggtaatttc
     5401 atttccctta tggaatctag cgataggacg gttcgaaaag aagcatatca agctatgtat
     5461 agcacatatg aacaattcca gcatacttac gctaaaacat tacaaacaaa tgtgaaatct
     5521 caaaatttta aggctcgtgt gcatcattat caatcagcac gccaatcagc tctatctgcc
     5581 aattttattc cagaagaggt ctacgaaact ctaattaaga cagttaatca tcatttaccg
     5641 ctcttacatc ggtatatgaa gttgcgtcaa aaagttcttg ggctagatga tttgaagatg
     5701 tatgacgtat atacacccct ttctcaaatg gatatgagtt ttacttatga tgaagctcta
     5761 aaaaaatcag agaaggttct ggctattttt ggcgaagcat attctgaacg tgttcatcgt
     5821 gcttttactg aacgttggat tgatgtccat gttaacaaag gaaagaggtc aggcgcttat
     5881 tcaggtggat cttatgacac taatgctttc atgcttctta actggcaaga tactttagac
     5941 aatctttata cgctggtgca tgaaactgga catagtctac actcgacgtt tactcgtgaa
     6001 aatcaacctt atgtttatgg tgattattct attttcctag ctgaaattgc atcaacaaca
     6061 aatgaaaata tattaacaga aacccttttg aaagaagtta aagatgataa aaatcgcttt
     6121 gcaattctca atcattattt ggacggtttc aaaggaacaa tttttagaca aacacagttc
     6181 gctgaattcg aacatgccat tcatgtagct gatcaggagg ggcaagtact aactagcgaa
     6241 tacttaaaca acctttacgc tgagcttaat gaaaaatatt atggtttgac taaagaagat
     6301 aatcatttta ttcaatatga atgggcaaga attccacact tttactacaa ttattatgtt
     6361 ttccaatatg caaccggctt cgcagcagca aattatttag cagagcgtat tgtaaacggt
     6421 aatccggagg ataaagaggc ctacctaaat taccttaaag ctgggaattc agattaccct
     6481 ctaaacgtca ttgctaaagc cggtgttgat atgactagtg cagactatct ggatgcagct
     6541 tttagagtat ttgaagagcg tttagtggaa ctggaaaatc ttgtagcaaa aggcgtccat
     6601 aatgattaag ttgaaaatat agctattgaa gtaaaaccag tcaactagac tggtttttga
     6661 tagtgatcag ttattttata gtttgttttg agagtggttt tggtaaagtt agttagtgga
     6721 aaactcaata ggtggaaata cctacccaag ctatgatttt tatgagacca aaactgctat
     6781 tgtcaaaaaa gaagggaaat aggatgaaaa agcttacttt tatttgggat ttagatggga
     6841 cattaataga ttcgtatgta ccaattatgg aagctcttga agaaacctat cgtcattttg
     6901 gcttaatatt tgataaagaa ttaatccatg aatatatttt acaggaatca gtggggcaat
     6961 tattggtaaa cctttcagag gaagagcaaa tacctcatga aaaactgaaa gcatatttta
     7021 caaaagaaca agaaagtcga gattctaaaa tacatttaat gccatatgca aaagagattt
     7081 tagaatggac caaagaacaa gatattccca attttatgta tacacataaa ggagcaagta
     7141 cgcattcagt gttggaaacc ttgcagatct ctcattattt tgatgaaatt ttaactggtg
     7201 tttcgggatt cgagcgaaaa ccacatccac aagggattaa ttatttagtt aaacgatatt
     7261 ctttagataa atcaatgact tattacatag gagatcgtcc actagatttg gaggttgctc
     7321 aaaatgctgg tataaaatcc ataaacttaa ggttagagaa ttccaaagaa aactataata
     7381 tttcaagtct caaagatata atatcacttg atttcactcg tttggattaa atcattctca
     7441 gagttagaca ttttttgcta taataaaaaa gttattattt aaaatagaaa agaggagcca
     7501 aaagtggttc aatcttacag taaaaatgct aatcataata tgcgccgtcc agtagttaaa
     7561 gaagaaattg ttcaatatat gcgtcagcac caaaaacaaa ataatggctg tttagcagag
     7621 ttagaagcat ttgctaaaca ggaaaatatc cccattatac ctcatgaaac agcgacttat
     7681 tttcgttttt tgatgcaaac attacaacct aagcacattt tagaaatcgg aacagctatt
     7741 ggtttttcgg cccttttgat ggcagaaaac gctcctgaag caaagataac aacaatcgat
     7801 cgtaatgaag agatgattgc cttggctaag gaaaactttg caaaatatga taatcacaat
     7861 cagataaccc ttttggaagg cgatgctgtg gatgtgttac aaacattgga taaatcttac
     7921 gattttgtat ttatggattc tgctaagtct aaatatatcg tttttttacc tcaagtatta
     7981 aagcatttag atgttggagg agtagttgtt ttagatgata ttttccaagg tggagatatt
     8041 gcaaaaccta tcgatgaagt gagaaggggt cagcgaacta tctaccgagg tctgcaacgt
     8101 ttatttgact cgactttgca acatcctgat ttaactgcta ctttggtacc tttgggagat
     8161 ggtttattga tgattcgtaa aaatgcagat catattgtac tagaagatta aggcattttt
     8221 aagaaattat gatataataa tggagttaat gacaaaaagg agttataaat catgaaaaca
     8281 agatcaaaac ttgcagcagg ctttttgaca cttatgtcag ttgccacatt agctgcctgc
     8341 tcaggtaaaa catcaaatgg aacaaatgtt gttacaatga agggcgacac tattacagtc
     8401 tctgattttt atgatcaagt aaaaacatca aaagctgcac aacaatcaat gcttacattg
     8461 atcctctcac gtgtttttga tacacagtat ggtgataaag tttcagataa aaaagtatca
     8521 gaagcttata ataagacagc taaaggctat ggtaattcat tttcaagcgc actttcacaa
     8581 gcaggtttga ctccggaagg ttacaaacaa caaattcgca caactatgct tgtggaatat
     8641 gctgtaaaag aagcagctaa gaaagaatta acagaagcaa actataaaga agcatataaa
     8701 aactatactc ctgaaacttc tgtacaagta atcaaattgg atgcagaaga taaagctaaa
     8761 tctgtcctta aagatgtaaa ggctgatgga gctgattttg caaagattgc aaaagaaaaa
     8821 acaacagcta ctgataaaaa agttgagtat aaatttgatt ctgcagggac aagcctccct
     8881 aaagaagtta tgtcagcagc ctttaagcta gataaaaatg gtgtttcaga tgtggtttca
     8941 acggttgatt caacaactta taaaacaagt tactacatca ttaaagtaac tgataagaca
     9001 gagaaaaaat ctgattggaa atcttacaaa aatcgcttaa aagaagttat tcttaaggat
     9061 aaaacaagcg atagatcctt ccaaaataaa gtgatttcaa aagccttaga aaaagctaac
     9121 gttaagatta aagataaagc atttgcaggc atcttatcac aatatgctac aacaagtggt
     9181 tcatcatcac ttaaaaaata gttgaccaag tcttaaaaag actgtataat taatactatt
     9241 gagaatgaaa tttcaacgat tagatacaga aaagtgacgg ttgctgcgag tcatggataa
     9301 tcagaaaaag atgctactca atggggtaaa actaaaaata aatgagaatg acaagttcat
     9361 tgaatgaagg tggtaccgcg gttttcgccc ttcgtaatgg acttgtcttt tgctatttgt
     9421 caactttcga ggaggaagag atatggctac ttttcaaatt aaagaaaaaa tgttttctct
     9481 aggcggtaaa tttactatta cagacctgac tgggcttccc tgttatcacg ttgaaggaag
     9541 tttgttccct ctacctaaga cttttaaagt ctttgacgaa gaggaacatc tcattagtca
     9601 gattgagaag aaggttttaa gttttttacc aaagtttaat gtgacattag ccaatgggaa
     9661 tcatttcacg attaagaaag atttctcatt tttgaaacct cattacacca ttgaagacct
     9721 tgatatggaa gttaagggaa atttttggga tatggatttc caactcttaa aagacaatca
     9781 agttatagca aacatatctc aacagtggtt tcgtatgact tcaacttatc aggtagaagt
     9841 ttacagtgaa acttacaacg atttgacaat ttcgcttgtc atagcgatcg actatgtcaa
     9901 agagttagaa aaaaatgcat caaactaatt ttaggagaga ataatgaaag aattatcgtc
     9961 tgcacaaatc cgccaaatgt ggttggattt ctggaaatcc aaaggacatt ccgttgagcc
    10021 ttcagctaac ttggttcctg ttaatgaccc aacgcttctt tggatcaact caggtgttgc
    10081 aaccttgaaa aaatattttg atggttcagt gattccagaa aatccacgta ttaccaatgc
    10141 acaaaaatca attcgtacta atgatattga aaatgttggt aaaacagcac gtcaccatac
    10201 tatgtttgaa atgcttggta acttctcaat tggagactat ttccgtgatg aagctattga
    10261 gtggggattt gaactcttga caagtcctga atggtttgac ttccctaaag acaagctcta
    10321 catgacttat tacccagatg acaaggattc atataaccgt tggattgctt gtggcgttga
    10381 accaagtcac ttggtgccaa tcgaggataa cttctgggaa atcggtgctg gtccttcagg
    10441 tccagatact gagattttct tcgaccgtgg tgaagatttt gatccagaaa atattggact
    10501 tcgtcttttg gctgaagata tcgaaaacga tcgttacatc gaaatctgga acattgttct
    10561 ctcacaattc aatgctgacc cagccgtacc acgttcagaa tacaaagaat tgccaaacaa
    10621 aaacattgat acgggtgcgg gtcttgaacg tcttgcagct gttatgcaag gggcaaaaac
    10681 aaactttgaa actgaccttt tcatgcctat catccgtgaa gtagagaaat tgtcaggtaa
    10741 aacttacgat ccagatggcg acaacatgag cttcaaggtt atcgctgatc acatccgtgc
    10801 gctttcattt gccatcggtg atggtgcgct tcctggaaat gaaggtcgtg gttatgttct
    10861 tcgtcgtctt ctccgtcgtg cggttatgca cggtcgccgt cttggcatca acgaaacttt
    10921 cctttacaaa ttggttccga ctgttggaca aatcatggaa agctactatc cagaagtgct
    10981 tgaaaaacgt gattttattg agaaaatcgt taaacgtgag gaagaaacat ttgctcgtac
    11041 tatcgatgct ggtagcggtc acttagattc attgcttgcg cagcttaagg ctgaaggtaa
    11101 agacacactt gaaggtaaag atatcttcaa actttatgat acttatggat tcccagttga
    11161 attgacagag gaattggcag aagatgcagg ctataagatt gaccacgaag gctttaagtc
    11221 agccatgaaa gaacaacaag accgtgcgcg tgcagctgtt gttaagggtg gttcgatggg
    11281 gatgcaaaat gaaaccctag ctggtattgt tgaagaatca cgattcgaat acgacacata
    11341 tagtcttgaa tcaagtcttt cagttatcat tgctgataat gaacgtactg aggctgtttc
    11401 agaaggtcaa gcccttcttg tctttgctca aacaccattc tatgctgaaa tgggtggaca
    11461 ggttgctgac acaggtagaa tcaaaaatga taagggtgac acagttgctg aggttgttga
    11521 tgttcaaaaa gcaccaaatg gtcaacctct acacactgta aacgttttgg catcactttc
    11581 agttggaaca aactacacac ttgaaatcaa caaagagcgt cgtttggctg ttgagaaaaa
    11641 ccacacagca actcacttgc tccatgcagc ccttcacaat gttatcggtg aacacgcaac
    11701 tcaggctggt tcattgaacg aagaagaatt cttgcgcttt gattttactc actttgaagc
    11761 agtaagcaat gaggaacttc gtcacattga acaagaagtt aatgagcaaa tttggaacgc
    11821 tcttacaatc acaacgactg aaactgacgt tgaaaccgca aaagagatgg gagcaatggc
    11881 actttttggt gagaaatatg gtaaagtggt tcgtgtggtt caaattggta attattctgt
    11941 tgaactttgc ggtggaactc acttaaataa ttcttcagaa atcggtctct tcaagattgt
    12001 caaagaagaa ggtattggtt caggcactcg tcgtattatt gcagttactg gtagacaagc
    12061 ttttgaagct tatcgtaacc aagaggatgc actaaaagag atcgctgcta ctgtaaaagc
    12121 tccgcaattg aaagatgcaa cagctaaagt acaagctctt agcgattcac ttcgtgatct
    12181 tcaaaaagaa aatgtagagc ttaaagaaaa agcagcagca gcagcagctg gtgatgtctt
    12241 taaagatatt caagaagcta agggcgtgcg cttcattgct agtcaagttg atgttgcaga
    12301 cgcaggtgca cttcgtacat ttgctgataa ctggaaacaa aaagactact ctgatgtgct
    12361 tgttctcgta gcagctattg gtgagaaggt taatgtcctt gttgcaagca aaactaaaga
    12421 tgtccacgct ggtaacatga tcaaaggatt ggcaccaatt gtagcaggtc gtggtggtgg
    12481 taaaccagac atggctatgg caggtggtag cgatgcaagt aaaattgcag agctgctagc
    12541 agcagttgca gaaaatttat aagaagtacc tctcatttag ttagtaaata aaacaggcaa
    12601 agggcagaac atcgttctgt tcctttgtgt atataggaga aatatggttg gtttaggaac
    12661 agttatcaat gttattctta ttatagttgg aggctttgta ggtttatttt tgaaaaattt
    12721 tttgaaagaa tcacttcaaa agagcctcat gcaagccatg ggagtcgcag ttttatttat
    12781 ttcaatatca ggtgttttgg agaaaatgat gttagtcgaa aagtcacatc tcatctctaa
    12841 tcatacaaat atgatgatta ttactcttgc tctaggtacg gtacttggag agttgctatc
    12901 actagattcg tatattgata gatttgggga ctatcttaag caaaaaacag gaagtggtaa
    12961 cgatattaaa tttgtggaag cattcgtcac ctcaacttgt actgtttgta ttggagcaat
    13021 ggccgttgtt ggtagcattc aagatggtat agcagcagat cattcgattt tatttgctaa
    13081 gggaatgtta gacatgatta ttattgcaat tatgacagtg tctttaggga aaggggctct
    13141 attttctgca cttcctgtcg ctttactaca aggtagcctt acgattgttg cttttttcat
    13201 ggggagccta cttaatccat catctttaga ttaccttaac ttagtaggaa atatgcttat
    13261 attctgtgtc ggtgttaatt tactctttaa cctaaatatt aaagttatca atatgttgcc
    13321 agcaattatt ttagctatat tatggggaag ttttatataa aagataacca agttttaact
    13381 caaacttggt ttttctttag acatatgtta ggatgaaaga agatatacta cggagaggat
    13441 ccaactcatg aatctattat tttctattga tgatatgtac gtagatcatt ttaaagtgat
    13501 gctttattcg cttgtccgac aaaccaagaa tcgtaagtta gagatatatg tcttacaaaa
    13561 aacattactc aaacgtcata cagaacttat tcaatatact caaaatttag aagttggcta
    13621 tcatccaata attgtaggta ccgaagtatt tgctcaggca cctacaactg atcgctatcc
    13681 agatacaata tattatcgac ttttggctca taaattttta cctgagacct tagataggat
    13741 actttattta gatgcagata tgctttgctt aaatgatttc tcaagtttat atgacatgga
    13801 gttaggtgat caactatatg cagcggcaag tcataatact gatggtaagt ttctagatta
    13861 tgtcaataaa ttacggttaa aaaatgtaga gctagaatcc tcttatttca atacaggggt
    13921 actcttgatg aacttgcctg ctatacgaaa agttgttcat caacaaacta tcttagatta
    13981 cataatgcaa aaccgtggtc gtctaatctt accagatcag gatatcttaa atggcttata
    14041 cgctaatttg gttaaaccaa tacctgatga aatttataat tatgatgcta ggtatagtct
    14101 tatttatcaa ttaaaaagtc gcaatgaatg ggatttagag tgggttatta accatactgt
    14161 ttttttacat tttgcaggac gagataaacc atggaaaaaa gattatagag gacgttactc
    14221 gggcctttat aagtttatgg ctaaagaggc tttaactaaa aattgaagat tta
//
LOCUS       DBJYSH010000052        13957 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.052, whole genome shotgun sequence.
ACCESSION   DBJYSH010000052 DBJYSH010000000
VERSION     DBJYSH010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 13957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13957
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.052"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            21..1208
                     /locus_tag="ACUBV3_001176"
     CDS             21..1208
                     /locus_tag="ACUBV3_001176"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HHU3847681.1"
                     /translation="MPKLIVSFLCILLSLTCVNSVQAEEHKDIMQITREAGYDVKDIN
                     KPKASIVIDNKGHILWEDNADLERDPASMSKMFTLYLLFEDLAKGKTSLNTTVTATET
                     DQAISKIYEISNNNIHAGVAYPIRELITMTAVPSSNVATIMIANHLSQNNPDAFIKRI
                     NETAKKLDMTKTHFYNPSGAVASAFNGLYSPKEYDNNATNVTTARDLSILTYHFLKKY
                     PDILNYTKYPEVKAMVGTPYEETFTTYNYSTPGAKFGLEGVDGLKTGSSPSAAFNALV
                     TAKRQNTRLITVILGVGDWSDQDGEYYRHPFVNALVEKGFKDAKNISSKTPVLKAVKP
                     KKEVTKTKTKSIQEQPQTKEQWWTKTDQFIQSHFVSILIVLGTIAILCLLAGIVLLIK
                     RSR"
     gene            complement(1280..2698)
                     /gene="sufB"
                     /locus_tag="ACUBV3_001177"
     CDS             complement(1280..2698)
                     /gene="sufB"
                     /locus_tag="ACUBV3_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017769002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HHU3847682.1"
                     /translation="MSEINEKVEPQPIDLGEYKFGFHDDVKPIYSTGKGLNEAVIREL
                     SAAKGEPEWMLDFRLKSLETFNKMPMQTWGADLSDIDFDDIIYYQKASDKPARDWDDV
                     PEKIKETFERIGIPEAERAYLAGASAQYESEVVYHNMKEEYDKLGIVFTDTDSALKEY
                     PELFKKYFAKLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDIPLQTYFRINNENTGQ
                     FERTLIIVDEGASVHYVEGCTAPTYSSNSLHAAIVEIFALDGAYMRYTTIQNWSDNVY
                     NLVTKRATAKKDATVEWIDGNLGAKTTMKYPSVYLDGEGACGTMLSIAFANKGQHQDT
                     GAKMIHNAPHTSSSIVSKSIAKGGGKVDYRGQVTFNKDSKKSVSHIECDTILMDDISK
                     SDTIPFNEIHNSQVALEHEAKVSKISEEQLYYLMSRGLSEAEATEMIVMGFVEPFTKE
                     LPMEYAVELNRLISYEMEGSVG"
     gene            complement(2798..3241)
                     /gene="sufU"
                     /locus_tag="ACUBV3_001178"
     CDS             complement(2798..3241)
                     /gene="sufU"
                     /locus_tag="ACUBV3_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HHU3847683.1"
                     /translation="MALSKLDNLYMAVVADHSKHPHHHGFLEGVEQVQLNNPTCGDVI
                     SLSVKFDGNIISDIAFAGNGCTISTASSSMMTDAVIGKTKEEALQLADVFSKMVQGDQ
                     NPKQEKLGDAEFLAGVSKFPQRIKCATLSWNALRKAIERDNQAEL"
     gene            complement(3228..4460)
                     /locus_tag="ACUBV3_001179"
     CDS             complement(3228..4460)
                     /locus_tag="ACUBV3_001179"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HHU3847684.1"
                     /translation="MTLLDSYKLKQDFPILNQLVNDEPLIYLDNAATTQKPNQVLETL
                     RAYYQNDNANVHRGVHTLAERATAQYETAREKARQFLNAKSSKEILFTRGTTTGLNWV
                     AKFAESILERGDEVLISIMEHHSNIIPWQQACERTGAKLVYAYLKDGSLDLEDFYNKL
                     SSKTKFVSLAHISNVLGCITPVKAIAERVHQVGAYMVVDGAQSAPHMAIDVQDLDCDF
                     FALSGHKMLGPTGIGVLYGKESILDKMPPVEFGGEMIDFVYEQSATWKELPWKFEAGT
                     PNIAGAIAFGEALDYLTDVGMDEIHQYEQSLVSYVLPKLQAIDGLTIYGPSDAESHVG
                     VIAFNLEGLHPHDVATAMDYEGVAVRAGHHCAQPLINHLGIHSAVRASFYFYNTKEDC
                     DKLVDAIQKTKEFFNGTL"
     gene            complement(4462..5724)
                     /gene="sufD"
                     /locus_tag="ACUBV3_001180"
     CDS             complement(4462..5724)
                     /gene="sufD"
                     /locus_tag="ACUBV3_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019805197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HHU3847685.1"
                     /translation="MSKEAILNFLQAKGEPTWLQELRLKAFEKIEELELPVIERVKFH
                     RWNLGDGTILENDYTANIPDFTELGNNPKLVQIGTQTVLEQVPMELIEKGVVFTDFYS
                     ALEEIPEVIERYFGKARPFEEDRLAAYHTAYFNSGAVLYIPDNVEITQPIEGLFYQDN
                     QSKVPFNKHILLIVGKNAKVSYLERFESIGDGTERTSANISVEVIAQAGSQIKFASID
                     RLGENVTTFISRRGRHSSDATIDWALGVMNEGNVVADFDSDLIGDGSHANLKVVAASS
                     GRQVQGIDTRVTNYGCNSVGHILQHGVILERGTLTFNGIGHIIKGAKGADAQQESRVL
                     MLSDKARSDANPILLIDENDVTAGHAASIGQVDPEDLYYLMSRGLNQKTAEQLVIRGF
                     LGTVIAEIPVKEVRDEMIAVIDTKLEKR"
     gene            complement(5761..6531)
                     /gene="sufC"
                     /locus_tag="ACUBV3_001181"
     CDS             complement(5761..6531)
                     /gene="sufC"
                     /locus_tag="ACUBV3_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HHU3847686.1"
                     /translation="MSVLEIKNLHVSIEDKEILKGLNLTLKTGEIAAIMGPNGTGKST
                     LSAAIMGNPNYEVTAGEILFDGEDILELEVDERARLGLFLAMQYPSEVPGITNAEFIR
                     AAMNAGKADDDKISIRQFITKLDEKMELLGMKEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPKFALLDEIDSGLDIDALKVVSKGVNEMRGEGFGAMIITHYQRLLNYITPDKVHV
                     MMDGKVVLSGGPELAVRLEKEGYAQIAEELGLEYKEEV"
     gene            complement(6696..7856)
                     /locus_tag="ACUBV3_001182"
     CDS             complement(6696..7856)
                     /locus_tag="ACUBV3_001182"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000612108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0070085 - glycosylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HHU3847687.1"
                     /translation="MIPFTIEYIFVLIGAFLLSIILTPIIRVISLKVGAVDKPNARRI
                     NKVPMPSSGGLAIFLSFVVTTLFFMPMAASRHFIEVSYFHYILPVIIGGLVVTTTGFI
                     DDIFELRPRYKMLGIIIAAVIIWKFTHFRFDSFKIPIGGPLLEFGPILTFFLTVLWII
                     SITNAINLIDGLDGLVSGVSIISLATMAVVSYFFLPKIDFFLTLTIVILIASIVGFFP
                     YNYHPAIIYLGDAGALFIGFMIGVLSLQGLKNSTAVAVITPVIILGVPILDTAVAIVR
                     RKLSGKKISEADKMHLHHRLLSMGFTHRGAVLVVYGIAIIFSLIALLLNVSSRIGGIF
                     LLLALLLAMEIFIEGLNIWGENRTPLFNLLKFIGNSDYRQSVIAKYSDKHQK"
     gene            complement(7858..8613)
                     /gene="mecA"
                     /locus_tag="ACUBV3_001183"
     CDS             complement(7858..8613)
                     /gene="mecA"
                     /locus_tag="ACUBV3_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000425372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HHU3847688.1"
                     /translation="MEMKQISETTLKITISMEDLEDRGMELKDFLIPQEKTEEFFYSV
                     MDELDLPENFKNSGMLSFRVTPKKDRIDVFVTKSELSKDLNLEELADLGDISKMSPED
                     FFKTLEQSMLEKGDTDAHAKLAEIENMMDKATQEVVEENVSEEQPEKEVETIGYVHYV
                     FDFDNIEAVVRFSQTIDFPIEASELYKNGKGYHMTILLDLENQPSYFANLMYARMLEH
                     ANVGTKTRAYLKEHSIQLIHDDAISKLQMIEMG"
     gene            complement(8733..9572)
                     /locus_tag="ACUBV3_001184"
     CDS             complement(8733..9572)
                     /locus_tag="ACUBV3_001184"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HHU3847689.1"
                     /translation="MLIIELLKALFLGVVEGVTEWLPVSSTGHLILVQEFMKLNQSKS
                     FVEMFNIVIQLGAIMAVIVIYFKRLNPFQPGKSAREIRLTWQLWLKVVIACIPSILIA
                     LPFDNWFEAHFNFMIPIAIALIFYGFVFIWVEKRNAHLKPQVTELASMSYKTAFLIGC
                     FQVLSIVPGTSRSGATILGAIIIGTSRSVAADFTFFLAIPTMFGYSGLKAVKYFLDGN
                     VLSLDQSLILLVASLTAFVVSLYVIRFLTDYVKRHDFTIFGKYRIVLGSLLILYWLVV
                     HLF"
     gene            complement(9618..11492)
                     /locus_tag="ACUBV3_001185"
                     /pseudo
     CDS             complement(9618..11492)
                     /locus_tag="ACUBV3_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710504.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 family protein"
     gene            11628..13178
                     /locus_tag="ACUBV3_001186"
     CDS             11628..13178
                     /locus_tag="ACUBV3_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HHU3847690.1"
                     /translation="MKKILLSLFTALLITFGGMTSIQADEYLRVGMEAAYAPFNWTQN
                     DNTNGAVPIEGTDQYANGYDVQVAKKLAKKLNKKVVVVKTKWEGLVPALTSGKLDMII
                     AGMSPTEERKKEINFSKPYYISEPTLVVNAEGKYTNAKNISDFKNAKVTAQQGVYLYN
                     LIDQINGVKKEVAMGDFNQLRQAVESGVVDAYVSERPDATSAQTANPKLKMIELHQGF
                     KTSDADTNISVGMRKGDNRINQVNQVLESISRDKQIALMDKMIKEQPSVKKEKNGKPN
                     FFEQMATILKNNGSQFLRGTATTLLISMVGTIVGLFIGLLIGVFRTAPKSDNKLKAAL
                     QKLLGWLLNIYIEVFRGTPMIVQSMVIYYGTAQAFGVSLDRTLAAIFIVSINTGAYMS
                     EIVRGGIFSVDKGQFEAATALGFTHGQTMRKIVLPQVVRNILPATGNEFVINIKDTSV
                     LNVISVVELYFSGNTVATQTYQYFQTFTIIAIIYFILTFTVTRILRYIEKRFDSDNYT
                     TGANQLQV"
     gene            13188..13928
                     /locus_tag="ACUBV3_001187"
     CDS             13188..13928
                     /locus_tag="ACUBV3_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003063284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HHU3847691.1"
                     /translation="MTQAILEIKHLKKSYGSNEVLKDISLSVNKGEVISIIGSSGSGK
                     STFLRSINLLEEPSGGEILYHGHNVLEKGYDLNNYREKLGMVFQSFNLFENLNILENA
                     IVAQTTVLKRERQEAEKIAKENLNAVGMTEQYWKAKPKQLSGGQKQRVAIARALSVNP
                     EAILFDEPTSALDPEMVGEVLKTMQDLAKSGLTMIIVTHEMEFAKEVSDRVIFMDKGI
                     IAEQGTPKQLFENPTQERTKEFLQRFLK"
BASE COUNT         4595 a         2772 c         2216 g         4374 t
ORIGIN      
        1 aaagttttca ggagttacct atgcctaaat taatcgtatc tttcctctgc attttattat
       61 ccctgacttg tgtaaactct gtgcaagctg aagaacataa agatattatg caaattaccc
      121 gagaagccgg atatgatgtt aaagatatta ataaacctaa agcgtctatc gttattgaca
      181 ataaaggtca tattttgtgg gaagataacg ctgatttaga acgtgatccc gctagcatgt
      241 ctaaaatgtt tactttatat ttactatttg aagacttagc taaaggaaaa acaagcctca
      301 acaccacagt gactgcaaca gaaacagacc aagccataag taagatttat gaaattagta
      361 ataacaatat tcatgctggg gttgcttatc ctattcgtga actgattact atgacggctg
      421 tcccgtcatc taatgtagca actattatga ttgctaacca cttatcacaa aacaatcctg
      481 acgccttcat taaacgaatc aatgaaaccg ccaagaaact cgatatgaca aaaactcact
      541 tttataaccc tagtggggcg gtagcaagtg cttttaatgg actttactcc ccaaaagaat
      601 acgataacaa tgctactaac gttacgactg cacgtgatct atcaatttta acctatcatt
      661 tccttaaaaa ataccctgat atactgaact atacaaaata tcctgaagtc aaggccatgg
      721 tcggaactcc ttatgaagaa acatttacaa cttataacta ctctaccccc ggcgctaaat
      781 ttggattaga aggagtagat ggcttaaaaa ctggttctag ccctagcgct gcttttaatg
      841 ccttagttac agctaaacgc cagaatactc gtttgataac tgtgatttta ggcgttggcg
      901 attggtcaga ccaagacgga gagtactatc gtcatccgtt tgtcaacgct cttgtagaaa
      961 aaggttttaa agacgctaaa aatatttctt ctaaaactcc tgtattaaaa gccgttaaac
     1021 ctaaaaaaga agttactaaa accaaaacca aatctattca agaacagcct caaacaaaag
     1081 aacagtggtg gacaaaaaca gatcaattta ttcaatcaca ttttgtatct attttaattg
     1141 ttctgggaac catcgctatc ctttgtcttt tagctgggat agtattactt ataaagcgct
     1201 ctagataata actgtgctaa tataaaagcc cccttcatca ataacatagg ggctttttat
     1261 tatctcttga aatcttcaat taaccaactg aaccttccat ttcataggaa attaaacgat
     1321 ttaactctac cgcatattcc attggtaatt ctttcgtaaa gggctcaaca aaccccataa
     1381 caatcatttc tgttgcttca gcttcagata aacctcgact catcaagtag tacagttgct
     1441 cttcagaaat cttagacacc tttgcttcat gctctaaagc aacctgtgaa ttatgaatct
     1501 cattaaacgg tatggtatct gattttgaaa tatcatccat caatatggtg tcacattcta
     1561 tatgtgacac tgattttttg gaatctttat taaatgtcac ttgacctcga taatcaactt
     1621 ttcccccacc cttagcaatt gatttagaga caatggatga actagtatgg ggagcattat
     1681 gaatcatctt tgcacccgta tcttggtgtt gtcctttgtt tgcaaaagca atagacaaca
     1741 tcgtgccaca tgctccttca ccatcaaggt aaaccgatgg gtatttcatt gttgttttag
     1801 cacctagatt tccatctatc cactcaactg ttgcatcttt tttagcggta gcacgttttg
     1861 tcactaaatt atagacatta tcggaccaat tttgaatagt cgtatagcgc atataagctc
     1921 catcaagtgc aaaaatttca actatagctg catgtaaact atttgaagaa taagttgggg
     1981 cggtacaacc ttcaacgtag tgaacacttg ctccctcgtc aacaataatg agagtacgtt
     2041 caaattgtcc agtattttca ttattaatac ggaagtaagt ttgaagtgga atatccacct
     2101 taacaccttt agggacataa ataaatgttc cacctgacca tacagcagag ttcagagcag
     2161 ctaatttatt atctgttgga gggacaagtt tagcaaaata ttttttgaat agctctgggt
     2221 actctttaag tgcagagtca gtatccgtaa aaacaatacc cagcttatca tattcttctt
     2281 tcatattatg ataaaccact tctgattcat attgtgctga tgctcctgca agataagctc
     2341 tttcagcttc tggaatccca attctttcaa aagtttcttt gattttttct ggaacatcat
     2401 cccaatcacg cgcaggttta tcagatgctt tttgataata aataatatca tcaaaatcaa
     2461 tatctgataa atctgctccc caggtctgca ttggcatttt attaaacgtt tccaaggatt
     2521 ttagacgaaa gtccaacatc cactcaggtt cacccttagc tgcagataac tcacgaataa
     2581 cagcctcatt tagacctttt cccgtagaat aaattggctt tacatcgtca tgaaatccaa
     2641 atttatattc cccaagatct attggctgtg gttctacttt ttcatttatt tcggacatac
     2701 ttatcctttc tttaatattt aaatttaaga cattcttcta ctagactacc ttatgtcagt
     2761 aaaagttgtt taaatgtaat tccttcaact atggttgtca gagttctgcc tgattatctc
     2821 tctcaatagc ctttcttaaa gcattccacg aaagcgtagc acatttgata cgttgtggaa
     2881 attttgatac cccagctaga aactctgcat ccccaagttt ctcttgtttt ggattttgat
     2941 cgccttgaac cattttggag aatacatcag caagctgcaa tgcttcttct tttgttttcc
     3001 caataactgc atctgtcatc atgcttgatg aagccgtaga aattgtacaa ccattaccag
     3061 caaatgcaat atctgaaata atatttccat caaatttaac agataaggaa ataacatctc
     3121 cacatgtagg attatttaac tgcacttgtt caacgccttc taaaaatcca tgatgatgag
     3181 ggtgttttga atgatcagct accactgcca tgtagaggtt atctaattta gagagtgcca
     3241 ttgaaaaact ccttagtttt ttggatagcg tctaccaatt tgtcgcaatc ctctttggta
     3301 ttatagaaat agaaacttgc tcgaaccgcc gaatggatac caagatgatt aataaggggt
     3361 tgtgcacaat gatgacctgc acgaacagcc actccttcgt aatccattgc agttgccaca
     3421 tcatgtgggt gtaaaccttc taaattgaag gcaataacgc ctacatgaga ttcagcatca
     3481 ctaggtccgt aaatagttaa accatcgata gcctgtaatt taggcaacac ataagaaact
     3541 aaactttgtt catattgatg aatctcatcc ataccaacat ccgttaaata atctaaagct
     3601 tctccaaaag cgatcgctcc agcaatatta ggcgttccag cttcaaactt ccagggcaat
     3661 tccttccatg ttgcactttg ctcatagaca aaatcaatca tttcaccacc aaattcgaca
     3721 ggtggcatct tatccaaaat gctttctttc ccataaagaa caccaatacc agtaggacca
     3781 agcatcttat ggccagataa ggcgaagaaa tcacaatcaa ggtcttgaac atcaatcgcc
     3841 atatgtggcg ctgattgagc tccatcaaca accatgtatg ccccaacttg atgcacacgt
     3901 tctgctattg cttttactgg tgtaatacag ccaagtacat tcgagatatg tgccaaacta
     3961 acaaatttag ttttagagga taatttatta tagaaatctt ctaaatctaa agaaccatct
     4021 tttagatagg cataaaccaa tttagcacca gttctctcac aagcttgttg ccatgggata
     4081 atattagaat gatgctccat aatggaaata agtacctcat cacctcgttc taaaatactt
     4141 tcggcaaact tagctaccca atttaatcct gttgtggtgc cacgtgtgaa taaaatttct
     4201 ttacttgatt tagcatttaa aaactgcctt gctttttcac gagcagtctc gtactgagca
     4261 gtcgctcgtt ctgctaaagt atgaacacca cgatggacat ttgcattgtc gttttggtag
     4321 taagctcgca aagtttctaa aacttgattc ggtttttgtg tcgttgctgc attatctaaa
     4381 tagataagcg gctcatcatt aactaattga tttaaaattg ggaaatcttg ctttagttta
     4441 taactatcta gtaatgtcat attaacgttt ttctagtttt gtatcaataa cggcaatcat
     4501 ttcatcgcga acttctttta ctggtatttc agcaatgact gttcctaaaa atccacgaat
     4561 aactaattgc tctgctgttt tttggttcaa gccacggctc atcaaataat ataaatcctc
     4621 tgggtcaacc tgaccaattg aggctgcgtg accagctgta acatcatttt catcaatcaa
     4681 aaggatagga ttagcatctg aacgtgcttt gtctgataac atgagaactc ggctttcttg
     4741 ttgggcgtct gctccctttg caccttttat aatatgtccg ataccgttaa aagtaagagt
     4801 cccacgctct aaaataaccc catgctgtaa aatatgtcct actgaattac agccgtagtt
     4861 tgtaacacgc gtatcaattc cttgaacttg acgccctgac gaggcagcaa ctacttttaa
     4921 attagcgtgg cttccatcac caattaaatc agaatcaaag tcagcaacaa cattgccctc
     4981 attcatcacc ccaagggccc aatctattgt tgcatcgcta ctgtgacgac cacgacgact
     5041 tataaacgtt gtcacatttt ctccgaggcg atcaattgaa gcaaatttaa tttggctacc
     5101 tgcttgggca attacttcta cagaaatatt agcacttgtt cgttctgtcc catcaccaat
     5161 tgactcaaat cgctctaagt aactaacttt cgcattcttt ccaacaataa gaaggatatg
     5221 cttattaaat ggtactttag attgattatc ttgataaaaa agaccttcga ttggttgagt
     5281 aatttcaaca ttatctggaa tataaagcac tgcaccactg ttgaagtaag ctgtgtgata
     5341 ggcagcaagt ctatcttctt caaaaggacg tgctttccca aagtagcgct ctatgacttc
     5401 tggaatttct tccaaagccg aatagaaatc tgtaaagaca acgcctttct ctattaattc
     5461 cataggaact tgttcaagga cagtttgggt cccaatttga accaacttgg gattgtttcc
     5521 taactctgtg aaatctggaa tgttagctgt atagtcattc tctaaaatag ttccatcacc
     5581 taaattccat ctatgaaatt ttacacgttc aattactggt aattctagtt cttctatttt
     5641 ttcaaaagcc tttagtcgta attcttgtaa ccaagtaggc tcacctttgg cttgtaaaaa
     5701 atttaatata gcttctttag acatttgtaa ctccttttct cttctagttg aagtgacaaa
     5761 ttagacttct tctttatact ctaaaccaag ctcttcagcg atttgagcat aaccttcttt
     5821 ctcaaggcga accgctaatt ctggtcctcc tgacaatact accttcccgt ccatcataac
     5881 gtgtacttta tctggtgtaa tgtaattcaa aaggcgttga tagtgtgtaa taatcatagc
     5941 accaaaacct tcaccacgca tttcattgac tccctttgat accactttaa gagcatcaat
     6001 atcaaggcca gagtcaatct catccaagag ggcaaactta ggctctaaca ttaggagttg
     6061 taaaatttca ttgcgcttct tctcaccacc tgaaaaacct tcgttaagat aacgttccgc
     6121 catttcttct ttcatgccaa gtagttccat cttttcatct aacttagtga taaattggcg
     6181 gatggaaatt ttatcatcat cagcctttcc cgcattcata gctgctcgga taaattcagc
     6241 attagtaatc cctggaacct ctgaagggta ttgcatggca agaaatagac ccaaacgagc
     6301 acgttcatcc acttctagct ctaaaatatc ttcaccatca aaaagaattt cacctgctgt
     6361 aacttcataa ttaggatttc ccataatagc tgctgaaagt gtcgacttcc cagtaccatt
     6421 tggacccatg atagcagcaa tttctcctgt tttcaacgtt aaattaagac cttttaaaat
     6481 ttctttatct tcaatagaaa catgaagatt tttgatttca agtacagaca ttgtacactc
     6541 cttttcttgg ttaacagaga atatgtgata tttctctatg gtttaaagaa gatattatag
     6601 ggcactcacc cgatattata ttctaaataa ttatagcaaa aatgaggtgt gactgctatc
     6661 taaatgatta aaactaaaaa agccttttca ggcttttatt tttgatgctt atcgctatat
     6721 ttagcaataa cactttgtct gtaatcactg ttaccaatga actttaagag attaaatagt
     6781 ggtgtccgat tctcacccca aatatttaaa ccttctataa aaatttccat tgctaatagt
     6841 agcgccaata gaagaaaaat tcccccaata cgactagaaa catttaaaag caaagcgata
     6901 agcgaaaaga taattgcaat accataaaca actaaaactg ctcctctatg tgtaaatccc
     6961 atagatagca atcggtgatg caggtgcatc ttatcagctt cagaaatctt ttttcctgat
     7021 aatttacgcc tcacaatagc aacagctgta tccaaaatag gaactcctaa aataatgaca
     7081 ggtgttataa ctgcaacagc tgtggagttt tttaaacctt gaagcgataa tacaccaatc
     7141 ataaatccta taaacaaggc accagcatca cctaggtaga taatggctgg atgataatta
     7201 taagggaaaa aaccaactat tgaggcgatt aaaataacaa tagttagcgt gagaaaaaaa
     7261 tctattttag gtaaaaagaa ataagaaacc actgccatcg ttgctagact gatgattgaa
     7321 acaccactaa ctagaccatc tagaccatca atcagattga tagcattagt gattgaaata
     7381 atccataaaa ctgtcaaaaa gaaagttaaa ataggaccaa actccaagag tggaccacct
     7441 attggaatct taaaactatc aaatctaaaa tgtgtaaatt tccatatgat aacagctgca
     7501 ataataattc ccaacatttt ataacgaggt cttaattcaa agatgtcatc tataaatcct
     7561 gttgttgtaa ctacgagacc gccaataatt acaggcaaaa tgtagtgaaa ataagagact
     7621 tctataaagt gtctactggc cgccattggc atgaaaaata atgttgttac aacaaaggag
     7681 agaaatattg ctaaaccacc actacttggc attggtactt tgttgattct tcgagcgttc
     7741 ggtttatcaa ccgctcctac cttcaaagat ataactcgaa taataggagt tagaatgatt
     7801 gataataaaa atgctccaat caatacaaaa atatactcaa ttgtaaaagg tatcatttta
     7861 tcccatctca atcatttgta attttgaaat agcatcatca tgaatcaatt gaatactatg
     7921 ctctttcaag taagctcgtg tttttgtgcc cacattagca tgttcaagca ttcgagcgta
     7981 catcaaatta gcaaagtagc ttggttggtt ttccaagtct agcaaaattg tcatatgata
     8041 gcctttacca tttttataga gttcagaagc ttctattgga aaatcaatcg tttgtgaaaa
     8101 tcgaactaca gcttcaatat tatcaaaatc aaagacatag tgaacatatc caatcgtttc
     8161 tacttccttt tcaggttgtt cttcagaaac attttcctca actacttctt gagttgcttt
     8221 atccatcata ttttcaattt ctgctaattt ggcatgggca tccgtatccc ctttttccaa
     8281 catcgattgt tccaatgttt taaaaaagtc ttctggagac atttttgaaa tgtcacccaa
     8341 atctgctaat tcttctaaat ttaaatcttt acttaattca gactttgtaa caaaaacatc
     8401 aatgcgatct tttttaggtg ttactcgaaa acttaacata ccactatttt taaagttttc
     8461 tggcaagtct aattcatcca tgacagaata gaaaaattcc tcagtcttct cctgagggat
     8521 taggaaatct ttcagctcca taccacgatc ttctaaatct tccatactaa ttgtaatttt
     8581 cagtgttgtt tcactgattt gtttcatttc catatcatca cctcatacga gtattcagtt
     8641 ttcttattat accgaaaaag ctatttttta gcaatttttc gcagcctaac acgctaaaaa
     8701 gctgaggaat actcagcttt ttatgttata ttttagaata aatgaacaac taaccagtag
     8761 aggatgagta aacttcctaa gactatacga tacttaccaa agatggtgaa atcgtgtcgt
     8821 ttgacatagt ctgtcaagaa acgaataaca tataaactaa ctacgaaagc tgtcagactt
     8881 gctactaaaa gtattaaaga ttggtctaaa ctcaagacgt taccatctaa aaagtattta
     8941 accgccttaa gtccactata accaaacata gttgggatgg caaggaagaa agtaaagtca
     9001 gcagcgaccg aacgactagt tccaataata attgctccta aaatagtagc tccagaacga
     9061 ctggttcccg gaacaatact gagaacctgg aagcatccaa tcaagaaagc tgtcttataa
     9121 gacatacttg ccaattcggt tacctgtggt ttgaggtgtg cattacgttt ttcaacccat
     9181 atgaagacaa aaccataaaa gattagggct attgcaatcg ggatcatgaa attaaaatga
     9241 gcttcaaacc aattatcaaa tggtaaagca attaaaatag atggaataca agctatcact
     9301 acttttaacc ataattgcca agttaaacga atttctctag cagatttacc tggctgaaat
     9361 gggttaaggc gtttaaaata aatgacaata actgccataa ttgccccaag ttgtataaca
     9421 atattaaaca tttcaacaaa ggatttgctt tggttcaact tcataaactc ttgaaccaag
     9481 attaaatggc cagtactaga aacaggtaac cattctgtca ctccctcaac gactcctaaa
     9541 aatagggctt tgagtaattc aattattaac ataataactc cataaatttt tctttattat
     9601 accatacttt tggaaaatta gaaggcaccg ccacctccgc cgcctgagaa accacctgaa
     9661 gaaccgcctg aattaacact aaaattagaa gcgtgactaa catcttcagt tatcgttgaa
     9721 aaatgattac tagattgatt gactgaaatc gcaaatcgat gactatcaat gtttgcaaat
     9781 ctttctggaa tatctatttg gttcactctg agtactttct caacacggtc agcgtagccg
     9841 aataaagtag cgtaaaccaa tattcgattc catagtacga tactttctaa ctctgaacga
     9901 tcaaacgatt tgatatccct tagcatgttc ctaaaactag tccattggta gatacgactt
     9961 gctccatctt cagttacaat ataaccgtta cttatttttc ttgtcatcat aaaaatgatt
    10021 ttattcaaca acaggataac aacaaacaat aagagataga tacagaaata ggtaaaataa
    10081 tttaaaaaaa gcgtataaat tataacaaca aaagaaataa gaggtgatag gtaactgaat
    10141 cttttcaacc tagataattc tttagactct tctgaagaca ttttacgata aggtgatgaa
    10201 attcccttgc ttctaagaga gtttatatta tctttagaaa tcaacctcgt gagacgcgta
    10261 attcttgata aggcattact tcctcggcgt ctcatttgag tttgcagatc tgaagccttg
    10321 tgctgctttt tcagttcacg tagtgtttct ttataactaa attggtaatt agaaaagagt
    10381 tggtctggct tcaaactcgt agaatccgca aaagcaaact ctataaaatc tatttctgca
    10441 tcagaagctc tatctaagtt agctagagat aacaagttat catcataatt caatactttt
    10501 ctatcaatca agtctaatag aattgactga atgagttgtt cttgactgat aaggtgagtt
    10561 ttcttctcct catcttgaaa atttttaaaa ctcatactat aaatgctttg agttaactct
    10621 agtggtgaaa ggtcctcagg tgcttcatat aaatgatgat ccttagggaa ttgcccgtat
    10681 ttattaactt tttttctaaa caggaacacc ctaattgaga tgaatagtgt cacaataata
    10741 aagaatgaag gtaataatat ccttaacaag aaacttagga taaaaccatg acgctctatc
    10801 atcttctctt aatgttcaat ttttttctta taattatttt tactgtttgt aggtagatta
    10861 aaataagatc tatcccaata accatgaaat tctaatcgtt tgtttacatt aaaagctgtc
    10921 aaatggtaac gattattatt ttgtcttatt ttaggaggag ttttaagata acccaagtgc
    10981 ccccaaagac gagaaagagc aacctttttg tcagttgata tccaaaaatc tactttctct
    11041 aacgttttat cccagtcgct aataggaatc cagttaagtt caccaacatc cttatgcata
    11101 aatagaacat tttttagttt ccactgaaca ttgactttaa cagtatcacc tgcttcacca
    11161 ccattaaaca ctttcaaacg gtagccatcc tccaaatctt ctatctggta actaactttc
    11221 cttactttac cattaattga aacttcaacc tgtggcttat tattaatatc aaaattgtca
    11281 ggtaacttac ccgccgtacc taatgtgaca tactgtccat tataggacga atcaaattgg
    11341 taagtaacct tctctgtaaa atcagcacta ttatcattgt gaatagttag attaccctca
    11401 taatgaggaa tgttatagtc cacctcatct gcttgaactg aaaccataaa aaaactaaga
    11461 gctaaacaaa tagccaaaaa acatttcttc attatcatcc tccctccttt gtttgtatac
    11521 cattttaaca taaaaataca attaatcgct ttcaataaga taatgatatt gaaatcgacc
    11581 ttgtttttcg gtaaaatagg aggaagtaac ttatcggagg ctgttgaatg aaaaaaatat
    11641 tactaagttt attcactgct ttgcttatca cctttggagg tatgacaagt atacaagcag
    11701 atgaatatct acgtgtcggt atggaggcag cctatgctcc tttcaactgg acacaaaatg
    11761 ataacacaaa tggtgccgtt cctattgaag gaactgatca atatgctaat ggttatgatg
    11821 tacaagtcgc taaaaagtta gctaaaaagt taaataaaaa agttgttgtt gtcaaaacaa
    11881 aatgggaagg ccttgttcca gcactaactt ctggaaaact cgatatgatt attgcaggaa
    11941 tgagtcctac cgaagaacgt aaaaaagaaa ttaatttttc aaagccttac tacatttcag
    12001 aacctactct agttgttaac gctgaaggaa aatatactaa tgctaaaaat attagtgatt
    12061 tcaaaaatgc taaagtaact gcccaacaag gagtctactt atacaacctc attgatcaaa
    12121 tcaatggtgt caaaaaagaa gttgccatgg gggactttaa tcaattaaga caagctgtag
    12181 aatctggtgt tgtagacgcc tatgtttcag aacgtccaga tgcaacttcc gctcaaactg
    12241 ctaatcctaa attaaaaatg attgaactgc accaagggtt taaaacatct gatgctgata
    12301 caaatatttc tgttgggatg cgtaaaggtg acaatcgtat taatcaagtc aatcaagttc
    12361 tcgaaagcat ttctagagat aaacaaatcg ccttgatgga taagatgatt aaagaacaac
    12421 catccgttaa aaaagaaaaa aatggaaaac caaacttctt tgaacagatg gcaactattt
    12481 taaaaaacaa tggttctcaa ttcctcagag gaacagctac aaccctccta atttctatgg
    12541 taggaactat cgtcggatta tttatcggcc ttctcatcgg tgttttccgt acagcaccaa
    12601 aatctgacaa taagttaaag gctgccttac aaaaattatt aggttggtta cttaacatct
    12661 atattgaggt cttccgcggg acacctatga ttgtacaatc gatggttatc tactacggaa
    12721 cagcacaagc ttttggagtt tcgctggacc gtacactagc agctatattt attgtttcaa
    12781 tcaatactgg tgcttatatg agtgaaattg tgcgtggtgg tattttctct gtcgataaag
    12841 gacaatttga agccgcaact gcacttggtt tcacccacgg acaaaccatg cgaaaaattg
    12901 ttctaccaca agttgtacgt aacatactcc cagctactgg taatgagttt gttatcaata
    12961 tcaaagatac ctctgtactg aacgttatct ctgttgtcga actctacttc tctggtaata
    13021 ctgttgcaac acaaacctac caatatttcc aaacattcac cattattgcg attatttact
    13081 tcatcttaac cttcaccgta acacgtatct tacgttacat tgaaaaacgt tttgattcag
    13141 ataattacac aaccggtgcg aatcaattac aagtctagga ggttggtatg acacaagcta
    13201 ttttagaaat taaacatttg aaaaaatctt acggaagcaa tgaagtgtta aaagatattt
    13261 cgctttcggt caacaaaggc gaagttattt ctatcattgg ttcttctgga tcaggaaaat
    13321 caactttcct acgttctatt aacctcttag aagaaccaag tggaggagaa atcttatatc
    13381 acggtcacaa tgttcttgaa aaaggttatg atttaaataa ttatcgtgaa aaacttggga
    13441 tggtctttca atcttttaac ctctttgaaa atttaaacat cttagagaat gctattgtcg
    13501 cacagactac tgttctaaaa cgtgaacgcc aagaagctga aaagattgct aaagaaaact
    13561 taaatgctgt tggcatgact gagcaatatt ggaaagctaa accaaaacaa ttatcaggtg
    13621 ggcaaaagca acgtgtcgca atcgcccgag cactttctgt taacccagaa gctatacttt
    13681 ttgatgaacc aacttcagcc ttggatccag aaatggttgg tgaggtcctt aaaacaatgc
    13741 aagatctagc taaatctgga ttaacaatga taattgtgac acacgagatg gaatttgcta
    13801 aagaagtatc agaccgcgtt attttcatgg acaaaggaat cattgcagag cagggtactc
    13861 ctaaacaact tttcgaaaat ccaacacaag aaagaactaa ggaatttcta caacgcttct
    13921 taaaataaaa tttattcaca tttaaatata gactatt
//
LOCUS       DBJYSH010000053        13610 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.053, whole genome shotgun sequence.
ACCESSION   DBJYSH010000053 DBJYSH010000000
VERSION     DBJYSH010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 13610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13610
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.053"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            complement(224..859)
                     /locus_tag="ACUBV3_001188"
     CDS             complement(224..859)
                     /locus_tag="ACUBV3_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HHU3847692.1"
                     /translation="MILREFCAENLTDLTRLDKAIISRVELCDNLAAGGTTPSYGVIK
                     EANQYLHEKGISVAVMIRPRGGNFVYNDLELRIMEEDILRAVELESDALVLGILTSNN
                     HIDTEAIEQLLPATQGLPLVFHMAFDVIPKSDQKKAIDQLVALGFTRILLHGSSNGEP
                     IIENIKHIKALVEYANNRIEIMVGGGVTAENYQYICQETGVRQAHGTRITQ"
     gene            992..2083
                     /gene="serC"
                     /locus_tag="ACUBV3_001189"
     CDS             992..2083
                     /gene="serC"
                     /locus_tag="ACUBV3_001189"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HHU3847693.1"
                     /translation="MTIYNFSAGPAVLPKPVLVKAQSELLNYQGSSMSVLEVSHRSKE
                     FDDIIKGAERYLRDLMGIPDNYKVIFLQGGASLQFSMIPLNIARGRKAYYHVAGSWGK
                     KAYTEAVKLSKTIPFEPILLASSEESVYDYIPEFDEKEIDPEAAYVHVTTNNTIEGTS
                     LYDIPKTNGVPVIADMSSNILAVKYKVEDFAMIYAGAQKNIGPAGVTVVIIREDMINE
                     EPTLSSMLDYKIQSDASSLYNTPPAYSIYIAKLVFEWVKSLGGVDAMEKANREKSGLL
                     YDYIDSSEFYSNPVRDKKSRSLCNIPFITINKDLDEKFVKEATERGFKNIKGHRSVGG
                     MRASLYNAFPKQGVIELIDFMKTFEAENA"
     gene            2152..2700
                     /locus_tag="ACUBV3_001190"
     CDS             2152..2700
                     /locus_tag="ACUBV3_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001167084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HHU3847694.1"
                     /translation="MQVRLAFPNEIDQIMLLIEEARAEIAKTGSDQWQKEDGYPNRND
                     IIDDILNGYAWVGIEDGMLATYAAVIDGHEEVYDAIYEGKWLHDNHRYLTFHRIAISN
                     QFRGRGLAQTFLQGLIEGHKGPDFRCDTHEKNVTMQHILNKLGYQYCGKVPLDGVRLA
                     YQKIKEKGETSIYREIDERNPM"
     gene            2762..3943
                     /locus_tag="ACUBV3_001191"
     CDS             2762..3943
                     /locus_tag="ACUBV3_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000232149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HHU3847695.1"
                     /translation="MVFSVKTFNNINQIGLQELGNRFQIDGDMSENPDAYIIRSQNLH
                     NQDFPSNLKAIARAGAGTNNIPIEEASAQGIVVFNTPGANANAVKEAVIAALLLSARD
                     YLGANRWVNTLTGTDIPKQIEAGKKAFAGNEIAGKKLGVIGLGAIGARIANDARRLGM
                     TVLGYDPYVSIETAWNISSHVQRVKEIKDIFETCDYITIHVPLTNETKHTFDAEAFSI
                     MKKGTTIINFARAELVNNQELFKAIETGVVKRYITDFGDKELLNQKGITVFPHVGGST
                     DEAELNCAIMASQTIRCFMETGEITNSVNFPNVHQIQTAPFRITLINKNVPNIVAKIS
                     TAVSELGINIDNIINRSKGDYAYTLIDLDETDNNKISTLIEEFEGDENIVRVRLIAKQ
                     Q"
     gene            3999..4475
                     /locus_tag="ACUBV3_001192"
     CDS             3999..4475
                     /locus_tag="ACUBV3_001192"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HHU3847696.1"
                     /translation="MLYQEFYQSPLGEIRLLADNLGLSGLYFVGQKYDMLAVNQEEIV
                     NMSNSYTLLGKKWLDAYFSQQNLPSIPLSLRGTAFQTRVRQELQKIPFGDTKTYGELA
                     KELNCPSAQAVGGAIGKNPISLIIPCHRVLGRYGQLTGYAGGLERKSWLLEYEKEK"
     gene            4477..4833
                     /locus_tag="ACUBV3_001193"
     CDS             4477..4833
                     /locus_tag="ACUBV3_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000287944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HHU3847697.1"
                     /translation="MYTFYEYPKCTTCRSAKKELTELGLTFEAIDIKSNPPKASLLKE
                     LLENSPYDLKKFFNTSGNSYRELGLKDKFDDLTLDQALDLLASDGMLIKRPLLVKDNK
                     ILQIGYRTKYKDLNLV"
     gene            complement(4874..5701)
                     /locus_tag="ACUBV3_001194"
     CDS             complement(4874..5701)
                     /locus_tag="ACUBV3_001194"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HHU3847698.1"
                     /translation="MKLISWNIDSLNAALTSESTRALMSRQVIDTLVAEDADIIAIQE
                     TKLSAKGPTKKHLEVLETYFPEYDLVWRSSVEPARKGYAGTMFLYRKGLNPIVSFPEI
                     DAPTTMDNEGRIITLELENCYITQVYTPNAGDGLKRLGDRQIWDIKYAEYLATLDSQK
                     PVLATGDYNVAHKEIDLANPSSNRRSAGFTDEERQGFTNLLAKGFTDTFRYLHGDVPN
                     VYSWWAQRSRTSKINNTGWRIDYWLTSNRVADKITKSEMIHSGDRQDHTPIILEIEL"
     gene            complement(5778..6620)
                     /locus_tag="ACUBV3_001195"
     CDS             complement(5778..6620)
                     /locus_tag="ACUBV3_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HHU3847699.1"
                     /translation="MKKLLRWLPPVLFIIILIGMTILGKSYINKVTAHKIKLYNSRMT
                     PTILIPGSSATQERFNSMLAQLNQMGEKHSVLKLTVKKDNSIIYNGQISGNDHKPYIV
                     IGFENNEDGYSNIKKQTKWLQIAMNDLQKKYKFKRFNAIGHSNGGLSWTIFLEDYYDS
                     DEFDMKSLLTMGTPFNFEESNTSNHTQMLKDLISNKGNIPSSLMVYNLAGTNSYDGDK
                     IVPFASVETGKYIFQETAKHYTQLTVTGNNATHSDLPDNPEVIQYVAEKILKNEKGKL
                     PKPH"
     gene            complement(6604..7821)
                     /locus_tag="ACUBV3_001196"
     CDS             complement(6604..7821)
                     /locus_tag="ACUBV3_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HHU3847700.1"
                     /translation="MHSTIYKCHHCRYLIYKSFKDIFPFALIFSWIEILQKLVISDDS
                     FLAQTTKSSIYKNEWLYTVSNTLYDLSHLLIYFMASYFVASLVWRGLSNQTKESFSAS
                     LLGFATVWLLLTSVVPGTDYHYPSQPIWLMLALISYLFIFLTRLLQNRHHIFQYSMLA
                     ILLLISSILSHILYRYPNIDLEYLIQNHFSQWLGDGPHQLIPIMLWSILAIFLTLSGL
                     FVPDILFRPYSYFSVVSENLNAALSQHTDKIPYLYTFYTVKNSFAMFGGIGILLSLFL
                     AVLYESRKLQSKNYYKLTLLTLTPLIFDQNLPFLVGLPVILQPILFIPMVLTTIFAEA
                     FGALMLYLKFVDPAVYTVPSGTPSLLFGFLASNGDWRYLPVTAIILVVGFFIYRPFVK
                     IAFAKEEQYEKIT"
     gene            8157..8852
                     /locus_tag="ACUBV3_001197"
     CDS             8157..8852
                     /locus_tag="ACUBV3_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HHU3847701.1"
                     /translation="MSKKSHRQYQIYEGLRCAVALCFISGYINAFTYVTQGKRFAGVQ
                     TGNLLSFAIHLSNKHYSQALAFLLPIMVFMLGQSFTYFMNRWANKHQLHWYLLSSFAL
                     TQVAIVTIILTPFLPSSFTVAGLAFFASIQVDTFKSLRGAPYANMMMTGNIKNAAYLL
                     TKGLYEKNSDIFLIARNTIIIIGGFIFGVVCSTYFSSKLGEWSLSLILIPLLYVNLLL
                     GHEFYNLQVERHS"
     gene            complement(9010..9885)
                     /gene="tehB"
                     /locus_tag="ACUBV3_001198"
     CDS             complement(9010..9885)
                     /gene="tehB"
                     /locus_tag="ACUBV3_001198"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HHU3847702.1"
                     /translation="MVNTKTLLPYKTMPVWTAQSIPKAFLEKHNTKEGTWAKLTILSG
                     SLVFYQLSPDGEEISRHIFDASSDIPFVDPQVWHKVSPNSPDLSCYLTFYCQKEDYFH
                     KKYGLTRTHSEVIASAPLLSEKSNILDLGCGQGRNSLYLSLLGHQVTSVDSNGQSLVA
                     LENMALEEELPYNIKRYDINTAAIEGHYDFILSTVVFIFLNPDCISDIILQMQSHTQI
                     GGYNLIVSAMDTAENPCPLPFPFTFKEGQLKSYYNDWEIIKYNENLGELHRVDENGNR
                     LKLQFATLLARKLRS"
     gene            10028..12025
                     /gene="metG"
                     /locus_tag="ACUBV3_001199"
     CDS             10028..12025
                     /gene="metG"
                     /locus_tag="ACUBV3_001199"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HHU3847703.1"
                     /translation="MTEKKSFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRMMGFD
                     VQYLTGLDEHGQKIQQKAEEAGITPQEYVDGMAESVKTLWELLDISYDKFIRTTDTYH
                     EEAVAKIFEQLLAQGDIYLGEYTGWYSVSDEEFFTESQLAEVYRDENGNMIGGVAPSG
                     HEVEKVSEESYFFRMSKYADRLKAYYAEHPEFIQPDGRMNEMLKNFIEPGLEDLAVSR
                     TTYTWGVQVPSNPKHVIYVWIDALMNYISALGYGWSDDLSQYHKFWPADIHMIGKDIL
                     RFHSIYWPIMLMALDLPLPKRLVAHGWFVMQDGKMSKSKGNVVYPEMLVERFGLDPLR
                     YYLMRSLPVGSDGTFTPEDYVGRINYELANDLGNLLNRTIAMVNKYFDGEVPRFAVAT
                     DFDADLASVATDSIENYHKQMEAVDFPRALEAVWNLISRTNKYIDETAPWVLAKDETD
                     RDKLAAVMSHLVASLRVVAHLIQPFMMETSDAIMEQLGLGATFDLEKLTFADLPEGVR
                     VVAKGSPIFPRLDMEDEITYIKEQMNAGKAPVEKEWVPEEVELTSSKGQIKFEDFDAV
                     EIRVAEVIEVEKVEGSDKLLRFRLDAGDEGHRQILSGIAKFYPNEQELVGKKLQIVAN
                     LKPRKMMKKYVSQGMILSAEHDGKLTVLTVDSAVANGSIIG"
     gene            12221..13558
                     /gene="brnQ"
                     /locus_tag="ACUBV3_001200"
     CDS             12221..13558
                     /gene="brnQ"
                     /locus_tag="ACUBV3_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HHU3847704.1"
                     /translation="MVKKGFLTGLLLFGIFFGAGNLIFPPALGVASGQDFWPAILGFC
                     LSGVGLAIITLLLGTLTNGGYKTEMSEKFSPWFSLTFLVILYLTIGPLFAIPRTATVS
                     FEIGVAPIVGHSPIALLCFTACFFAAAYYLAIRPNGILDSVGKILTPVFAFLILSLVV
                     VGAIAYGNLESAKASADYAGKAFGSGVLAGYNTLDALAAVAFCLVATETLKKFGFKTK
                     KEYLSTIWIVGIVTSLAFSILYIGLGFLGNKFPVPADILADPNVNKGAYVLSQASYKL
                     FGNFGRYFLSIMVTLTCFTTTVGLIVSVSEFFDKNFRFGNYKLFATVFTLIGFLIANL
                     GLNAVITFSVPVLTLLYPIVIVIVLIILINKWLPLSKKGMSLTIGLVTLVSFVEVLAG
                     QWQEKTLTQLVGFLPFHTISMGWLVPMLIGIVFSLVLSDKQKGQAFDLEKFEG"
BASE COUNT         4396 a         2135 c         2599 g         4480 t
ORIGIN      
        1 taatctccga cttagctatt ggaataaaga agtaaggtgc ttccaatttc cttaagattt
       61 ttaatttcag ctcttttgaa taccattgca aattggattt tatttctgag agcaggttat
      121 aaaagatggt atgctcttat tccggttctt tcaggtttta caaaacatga aagaacttag
      181 tagtaaatat actaaagaaa cttatagccc tgtttcgaat tttttattga gttattctag
      241 taccatgggc ttgtctaacc ccagtttctt ggcaaatata ttgatagttt tcagcagtca
      301 caccgccgcc aaccataatt tcaatacgat tattagcata ttcaactaat gctttgatat
      361 gtttgatatt ttctataatt ggttcgccat tagatgaacc atgtaggaga attcttgtaa
      421 agcctaaagc aactaactga tctattgctt ttttttggtc agatttcggg ataacatcaa
      481 atgccatatg aaaaacaagt ggcaaacctt gggtagcagg tagaagttgt tcaatggctt
      541 cagtatcaat atgattattt gaagttaata ttcccaaaac aagagcatct gattctaact
      601 caacagcacg tagaatgtcc tcttccataa tacgtaattc taaatcattg tatacaaaat
      661 ttcctccacg gggacgaatc ataacagcga cactaatacc tttttcatgt aaatattgat
      721 tagcttcttt gataacaccg tagctaggag tagtgcctcc agcagcaagg ttatcacaaa
      781 gctcgacacg tgaaataata gctttatcta atctggttaa gtctgttaaa ttctctgcac
      841 aaaattccct caaaatcata aattactcct ttaaacttga ttgttcgcct ttaccattat
      901 atcacgaata aaaagcaaaa ttaatcaaaa aaatctttac aatattcaaa atactgtgct
      961 ataatcttac acaattatat ggaggtgtta tatgacaatt tataattttt ctgcaggtcc
     1021 tgcagtctta ccaaaaccag tgcttgtaaa agcccaatct gaactcctta attatcaagg
     1081 gtccagtatg agtgttttgg aggtatcgca ccgctccaaa gagtttgatg atattatcaa
     1141 aggtgctgag cgttacttaa gagatttgat gggaattccg gataattata aagtaatctt
     1201 tttacaaggt ggtgcatcct tacagtttag tatgattccc ttgaatattg ccagagggcg
     1261 taaagcttac tatcatgttg ctggttcgtg ggggaaaaaa gcttatacag aggctgtaaa
     1321 actatcaaaa acaattcctt ttgaaccaat tctattagcc tcatcagaag aatcagttta
     1381 tgattatatt ccagagtttg atgaaaaaga gattgatcct gaagctgcct atgttcatgt
     1441 gacaacaaat aataccattg aaggaacatc gctttatgat attcctaaaa ctaatggagt
     1501 gcctgttatt gcggatatgt cttctaatat cttagctgta aaatataaag tagaagattt
     1561 tgcaatgatt tatgcaggag ctcaaaaaaa tattggacct gctggtgtaa cggtagttat
     1621 tatccgtgaa gatatgatta atgaggaacc aactttatca tcaatgttgg attataagat
     1681 tcagtcagat gctagttctc tttataatac gccgcctgct tacagtattt acattgctaa
     1741 attagtcttt gaatgggtga aaagtctagg tggtgttgat gctatggaaa aagctaaccg
     1801 tgaaaaatca ggacttctct atgattatat tgattcctca gaattttata gtaatcctgt
     1861 cagagataaa aagagtcgtt ctctatgtaa cattcctttc ataactatta ataaagattt
     1921 agatgaaaaa tttgtaaagg aagcgacaga acgtggattt aaaaatatta aagggcatcg
     1981 ttcggtaggt ggtatgaggg ctagccttta taatgccttt cctaaacaag gtgtcatcga
     2041 attaattgat tttatgaaaa catttgaagc agaaaatgca tagtaaatgt tatcacgaaa
     2101 gaaaattaaa ccgttattat aagtctataa tatataaaag tgggtaatat aatgcaagtc
     2161 agattagctt ttccaaatga aattgaccaa attatgcttc ttattgagga agcgcgtgcc
     2221 gaaattgcta aaacaggaag tgatcaatgg caaaaagaag atggataccc taatcgtaac
     2281 gatattattg atgatatctt gaacggttat gcttgggttg gtattgaaga tgggatgcta
     2341 gcgacttatg cggctgtcat tgatggtcat gaagaggttt atgatgctat ttatgaggga
     2401 aaatggctcc atgataacca tcgctatctt acatttcatc gtattgctat ttcaaatcaa
     2461 tttcgtggta gaggactagc acagacattc cttcaagggt taattgaagg tcataaagga
     2521 cctgattttc gttgcgatac tcatgaaaaa aatgtcacta tgcagcatat tctaaataaa
     2581 ttaggctatc aatattgtgg taaagtacct ctagatgggg tacgattggc ttatcagaag
     2641 ataaaagaaa agggagaaac gagcatctat cgtgagattg atgaaagaaa tcccatgtaa
     2701 gaaaaaagaa aggggttatt ttagtcaatg atgactaaag agataacctt agtgaaaaag
     2761 aatggttttt agtgtcaaaa catttaataa tattaatcaa attggtttac aagagttagg
     2821 gaatcgtttc cagattgatg gggatatgtc agaaaatcct gatgcctata ttattcgtag
     2881 tcaaaatttg cataatcagg atttcccaag taacctcaaa gctattgcta gggcgggtgc
     2941 aggaacaaat aatattccta ttgaagaggc aagtgcacag ggaatagtcg tgtttaatac
     3001 cccaggtgca aatgctaatg ctgtaaaaga agcggtcatt gctgccttat tgctttcagc
     3061 tcgtgattat ttaggagcta accgatgggt taatactcta actggaacag atattcccaa
     3121 acaaattgaa gcaggaaaga aagcttttgc tggtaatgaa attgcaggga aaaaattggg
     3181 agttatcggc cttggtgcta ttggagctag aattgcgaat gatgctagac gcttaggaat
     3241 gacagttctt ggttatgatc cctatgtttc aattgaaaca gcttggaata tttcaagcca
     3301 tgttcaaagg gttaaagaga ttaaggatat ttttgaaact tgtgactata tcacaattca
     3361 tgttccctta acaaatgaaa ctaagcatac ttttgatgcg gaagcttttt caatcatgaa
     3421 aaaaggaact acgattatca actttgctcg tgcagaatta gtcaataacc aagagctatt
     3481 taaagcgata gaaactggtg ttgtcaagcg ctatattact gattttggag acaaagaatt
     3541 attaaaccaa aaaggaatta cagtctttcc tcatgtaggc ggatcaacag atgaggcaga
     3601 gctaaattgt gctattatgg caagtcaaac cattcgttgt tttatggaga caggagaaat
     3661 cactaattca gttaatttcc ccaatgtaca tcaaatccaa accgcaccat ttcgtattac
     3721 tttaattaac aagaatgttc ctaacattgt ggctaaaatt tcaactgctg tatcagagct
     3781 aggtataaat attgataaca ttattaaccg atcaaagggt gactacgcct acacactaat
     3841 agatttagat gaaacggata ataacaagat ttctacttta attgaagaat ttgaaggtga
     3901 tgagaatatc gtccgtgtac gtttaattgc aaaacaacaa taaagaacta aaaaagtcat
     3961 gaattgcttt tcatggcttt ttaagttatg cttatcatat gttatatcaa gaattctatc
     4021 agtctcctct tggagaaatt cgccttctag cagacaatct aggtttatca gggctttatt
     4081 ttgttgggca aaaatatgat atgctagcag tcaaccaaga ggaaattgtt aatatgtcaa
     4141 attcctatac tttacttggt aagaagtggt tagatgctta tttttcacag caaaatttac
     4201 ctagtattcc attatcattg agagggacag catttcagac gagagtacgg caggaattac
     4261 agaaaatccc ttttggagat accaaaactt atggagaatt agcgaaagag ctaaattgtc
     4321 catctgcaca ggctgttgga ggagcaatag gtaaaaatcc tatttctcta atcatacctt
     4381 gtcaccgtgt tttaggaaga tatggtcaat taactggtta tgctggtggc ttagaaagaa
     4441 aatcttggtt attagaatac gaaaaggaga aataatatgt atacgtttta tgaatacccc
     4501 aaatgtacca cttgtcgttc agcaaaaaaa gagttaactg aactcggctt aacttttgaa
     4561 gctattgata taaaaagcaa tcctcccaaa gcttcgttat taaaagaatt gttagagaat
     4621 tcgccttatg accttaaaaa gtttttcaat acttcaggaa attcttacag agaattaggc
     4681 ttaaaagata aatttgatga tttaactctt gaccaagctt tggacttatt ggcatcggat
     4741 ggaatgctta ttaagcgtcc attgctagtt aaagataata aaattcttca gattggctat
     4801 cgtactaagt ataaagacct caacttagta taaaaagatg attgagttat aactcaatca
     4861 tctttttttg ggtttatagt tcaatctcca aaataattgg tgtatgatct tgacgatcac
     4921 cagaatgaat catttctgac ttggtaattt tatctgcgac acggttactt gttagccaat
     4981 aatcaattct ccaccctgta ttgttgattt tggatgtacg acttcgttgt gcccaccagc
     5041 tataaacatt tggaacatcg ccatgaaggt aacgaaaagt atctgtaaaa cctttggcta
     5101 gtagatttgt aaagccttgg cgctcttcat ctgtgaatcc agcagaacga cgattactgc
     5161 taggattagc taggtctatt tccttgtggg ctacgttata gtcacctgtt gctagaacag
     5221 gtttttgact atccaatgtt gctaaatact cagcatattt aatatcccat atttgacggt
     5281 cacctaagcg ctttaggcca tcacctgcat ttggagtata gacctgtgtg atatagcagt
     5341 tttccaattc gagagtaata atgcgacctt cattatccat tgtagtagga gcatcgattt
     5401 cagggaagct aacaatagga tttaaccctt tacgatataa gaacattgtg ccagcatagc
     5461 ccttacgagc aggttcgact gatgatcgcc aaactaaatc atattctggg aagtaagttt
     5521 caagtacttc caaatgtttt ttagtaggtc ctttggcaga cagtttagtt tcttgaatcg
     5581 caatgatgtc ggcatcttca gcaactaaag tatcaataac ttggcgtgac attaaagcac
     5641 gtgttgattc acttgttaat gccgcattga gggaatcaat attccatgaa atgagtttca
     5701 taataatatc cttttttatt tagtttaatt cgcaatatat tatatcaaaa taccaggggc
     5761 gttgctattt tacagcttta gtgaggtttt ggtaatttac ctttctcatt tttaagaatt
     5821 ttttctgcga catattggat aacttcagga ttatcaggca agtcagaatg tgtagcatta
     5881 ttaccagtta ctgttagttg ggtatagtgt ttagcggttt cttggaaaat atatttacca
     5941 gtctccacac tagcaaatgg aacaatttta tcaccatcat atgaattagt tcctgccaaa
     6001 ttatatacca tgagacttga tggaatattt cctttattac tgattaaatc tttaagcatt
     6061 tgagtatgat ttgaggtgtt actttcttca aagttaaaag gtgttcccat tgttaacaat
     6121 gatttcatat caaattcatc agagtcgtaa taatcttcta ggaaaatagt ccatgataag
     6181 ccaccatttg aatgaccgat agcattaaaa cgtttaaatt tatatttctt ctgaagatca
     6241 ttcatagcaa tctgtagcca ttttgtttgt tttttgatgt tactataacc atcttcatta
     6301 ttttcaaatc caatgacaat gtagggtttg tggtcattgc cgctaatttg tccattgtag
     6361 ataatgctat tgtctttttt gacagttaac tttaaaacgc tatgtttttc tcccatttgg
     6421 ttgagctgtg ctaacatgct gttaaatcgt tcttgagtag cactggatcc tggaattaaa
     6481 atagtaggag tcattcgaga gttatagagt tttattttgt gagctgttac tttattgata
     6541 taggacttac ctaagatagt cattcctata aggataataa tgaaaagtac aggaggaagc
     6601 catctaagta attttttcat attgctcctc cttagcgaaa gcgattttga caaacggacg
     6661 ataaataaag aagccaacaa ctagaataat tgcagtgact ggaaggtaac gccaatcccc
     6721 atttgaagca agaaagccaa atagtagact aggtgtacca cttgggacag tataaactgc
     6781 agggtcgaca aattttaggt agagcataag agctccaaaa gcctctgcaa agatagttgt
     6841 taataccatc ggaatgaata atattggttg taaaatgaca ggtagaccta ctaaaaatgg
     6901 taaattttga tcaaaaataa gaggagttaa ggtcagtaat gtcaatttat agtaattctt
     6961 cgattgtagt tttctacttt catataaaac tgctaaaaat aaggataata ggattccaat
     7021 accgccaaac atagcaaagc tatttttaac agtataaaag gtatacagat aagggatttt
     7081 atctgtatgt tggctcaagg cagcatttaa attttcgcta actactgaaa aataggagta
     7141 aggacgaaat aaaatatctg gtacaaaaag acctgataag gttagaaaaa tagctaagat
     7201 agaccaaagc ataataggaa ttaattgatg tggcccatca cccagccatt gagaaaagtg
     7261 attttgtata aggtattcta aatcaatatt aggataacga tataagatat gtgacaaaat
     7321 agaagagatt aatagtagta tagctaacat actatactgg aaaatatgat gtcgattttg
     7381 aagcagtcga gttaaaaaga taaaaagata cgaaatcaaa gccagcataa gccaaatggg
     7441 ttggctagga tagtgataat ctgtacctgg aacaactgaa gttagaagca accaaacagt
     7501 ggcaaagcca agtaaacttg cagaaaaact ttctttagtt tggttagata agcctctcca
     7561 tactaaacta gccacaaaat aactagccat aaagtagatt agtaaatgag ataaatcata
     7621 aagagtattg gaaaccgtat ataaccactc gtttttataa atactggact ttgttgtttg
     7681 agctaaaaaa gaatcatctg atataacgag tttttgaagg atttcaatcc aagaaaaaat
     7741 gagagcaaaa ggaaagatat ctttaaaact tttataaatt agataacggc aatgatgaca
     7801 tttataaata gtgctgtgca taatctgtac tgacctccta ataggttgta tttattgtaa
     7861 caaatgttaa gaatggtgca agttttttgg tagtaaaatt aaaagtaact aattaaactt
     7921 caaattaagg gtaaatgact tatgagattt tctttatcct ttcataaagt gagattgctt
     7981 caatgctgag gttatgatat actaaaacta catttatcat tgcttaataa tttatttaga
     8041 actgttaggc tagctagtgc taatattaat actattttaa accaatctat tagaaaaaaa
     8101 gtgctgagca agattgccca gtcttatttt tgtttgccat aaagttagag gaaaatatgt
     8161 ctaaaaagtc tcaccgtcaa tatcaaatat atgaagggct caggtgtgcg gttgcactct
     8221 gttttatttc aggttatatc aatgctttta cttatgtaac acaaggaaaa cgttttgcag
     8281 gtgtacaaac agggaatttg ttatcttttg ctattcatct tagtaataag cattatagtc
     8341 aagcgcttgc ttttttgcta cctataatgg ttttcatgtt aggtcaatcg tttacttatt
     8401 tcatgaatcg ttgggcgaat aaacatcaac tgcactggta cctattatct agttttgcgt
     8461 tgacacaggt tgctatagta acaattatat tgactccttt cttaccatcg tcgtttactg
     8521 tggcaggact agccttcttt gcttcaatac aagtagatac ctttaaatcg ctcagaggag
     8581 caccttatgc taatatgatg atgactggta atatcaagaa tgctgcttat ttattaacaa
     8641 agggacttta cgaaaaaaat tctgatattt tcttaattgc tagaaatacg attattatta
     8701 ttggaggatt tatctttggt gttgtttgct cgacttattt ttcaagtaaa ctgggcgaat
     8761 ggagcttatc attaatatta attcctcttt tatatgtcaa cttgctgtta ggccatgaat
     8821 tttataacct acaagtagaa aggcactcct aatgtatggc gttttatgtt gaagagctat
     8881 actaaagctg attgttacaa taatgatttg aaatatcatc tctatatatt ttataggata
     8941 attatgtatg ggtttagaat atacttatta cctaaccaaa gataaaagtg agtagatttc
     9001 tactcacttt tatgatctta attttcgagc taataaagtc gcaaactgta attttaagcg
     9061 attaccgttt tcatccaccc tgtgcaattc acctaaattt tcattatatt tgatgatttc
     9121 ccagtcattg taataagatt tcaattgtcc ttctttgaag gtaaaaggga atggaagcgg
     9181 gcaaggattt tcagcggtat ccatagcaga aacgataaga ttgtatcctc caatctgagt
     9241 atgtgattgc atttgtaaaa taatatcaga aatgcagtcg ggatttaaaa atataaatac
     9301 cacagttgat aaaataaaat catagtgccc ttcaatagca gcagtattaa tatcatacct
     9361 ttttatattg taaggaagct cttcttctaa tgccatattt tctaaagcta caaggctctg
     9421 tccgtttgaa tcgacagaag tcacttgatg tcccagcagc gataaataaa gtgagtttcg
     9481 cccttgacca cacccaaggt ctaatatatt actcttctca gataagagag gtgcactggc
     9541 gataacctca gaatgtgtgc gcgtgagacc atatttttta tggaagtaat cttctttttg
     9601 gcagtaaaaa gttagatagc aacttaagtc tggactattc ggcgaaactt tatgccagac
     9661 ttgtggatca acaaaaggaa tatcactact agcatcaaaa atatgccgcg agatttcctc
     9721 tccatcagga gataactggt aaaatactaa agaaccactt agaatggtta gttttgccca
     9781 ggtgccttcc ttagtattat gcttttctaa aaatgccttt ggaatagact gtgcagtcca
     9841 aaccggcata gttttgtagg ggagaagtgt ttttgtatta accatatatt aactctctat
     9901 ttttaatacc tttattatag caaaagtaga tatattattc agagatatat ccagttgaaa
     9961 aagattttat gttataatgt atcatgataa aaaacacctc tcattagggt ggcttatagg
    10021 agaaatcatg acagaaaaaa aatcatttta cattactacc ccaatttatt acccatcagg
    10081 taaattgcac atcggttctg cctatacaac gatagcatgt gatgttttgg cacgttacaa
    10141 acgcatgatg ggctttgatg ttcagtattt gactggtctt gatgagcatg gtcagaaaat
    10201 tcaacaaaag gctgaagaag caggcattac accacaggaa tatgttgatg gcatggctga
    10261 aagtgtcaaa acattatggg aacttttaga tatttcctat gacaaattca tccgtacaac
    10321 agatacttac catgaagaag cggttgctaa aatttttgaa caattgttgg cacaagggga
    10381 catctatctt ggtgagtata ccggatggta ttctgtttca gatgaagaat tttttactga
    10441 aagccaatta gcagaagttt atcgtgacga aaacggtaat atgattggtg gtgttgcgcc
    10501 atctggtcat gaagttgaaa aagtttctga agaatcttat ttcttccgca tgagcaaata
    10561 tgcagatcgt ttaaaagcgt actacgctga gcatccagaa tttattcagc cagatggtcg
    10621 tatgaatgaa atgcttaaaa actttataga accaggttta gaagatttag cagtgtctcg
    10681 tacaacttac acatggggag ttcaggtacc atcaaatcca aaacatgtta tttatgtctg
    10741 gattgatgct ttgatgaact atatttctgc tcttggttat ggttggtctg acgatctcag
    10801 tcaatatcat aaattttggc cagcagacat tcatatgatt ggtaaagata ttcttcgttt
    10861 ccactctatt tattggccta tcatgttgat ggcgctggat ttgccattac caaaacgtct
    10921 agttgctcat ggttggtttg tgatgcaaga tggtaaaatg tctaaatcaa aagggaatgt
    10981 cgtttatcca gaaatgttag tagagcgttt tggtcttgat ccactccgtt attatctcat
    11041 gcgttcattg cctgttggtt cagacggaac ttttacgcca gaagattatg ttggacgtat
    11101 taactatgaa cttgctaatg atttaggaaa ccttctcaac cgcactattg ctatggttaa
    11161 caagtatttc gatggagaag taccaaggtt tgctgttgca actgactttg atgccgattt
    11221 agcttctgtt gcgacagata gtattgaaaa ctatcataaa caaatggaag cggtggattt
    11281 cccacgtgcc cttgaagctg tatggaatct tatttcacga actaacaaat atattgatga
    11341 aacagcaccg tgggtacttg caaaagacga aacagatcgt gataagttag cagctgtaat
    11401 gagtcattta gttgctagtc tacgtgttgt tgctcacctc attcaaccgt tcatgatgga
    11461 aacttccgat gccattatgg aacaacttgg tttaggggca acattcgatc ttgaaaaatt
    11521 gacttttgct gatctaccag aaggtgttcg agttgttgca aaaggttcac caattttccc
    11581 aaggttggat atggaagacg aaattactta tatcaaagaa caaatgaatg ctggaaaagc
    11641 accagtggaa aaggagtggg ttcctgaaga agttgaattg acatcaagca aaggtcaaat
    11701 caagtttgag gattttgacg ctgttgaaat ccgcgttgca gaagtaattg aagttgagaa
    11761 agtggaaggt tctgataaac ttcttcgttt ccgtctagat gctggtgatg agggccatcg
    11821 tcaaatctta tcagggattg caaaatttta cccaaatgaa caagagttag tagggaaaaa
    11881 actccaaatt gttgctaacc tcaaaccacg taaaatgatg aaaaaatatg tcagtcaagg
    11941 catgattctc tcagcagaac atgatggtaa attgacagtg ttgacggtcg attcagcagt
    12001 ggcaaatgga tcaattattg gttaatcgaa aagagcatta tgctcttttt ttgttttcaa
    12061 gaaaaaagtg actgaatagt tggaaaatta tgaaaatgac ctagaattta tttttatttt
    12121 tgatataata aaagacatcg attctaagca tttagataga atttgataag gagaatttat
    12181 ggaaagggtg taaatagatt atttacacta atggttgctt atggtaaaaa aaggcttttt
    12241 aacgggatta ctcttatttg gtatcttctt tggtgcaggg aacctcattt ttccaccagc
    12301 attaggtgta gcatctggac aagatttctg gccagcgatt ttaggatttt gtttgtcagg
    12361 agttggatta gctattatta ccctcctact aggcacacta actaatggtg gttataagac
    12421 agagatgtcg gaaaaattta gtccgtggtt ttctttaaca tttttagtta tactctacct
    12481 aactattggt ccactttttg ctattcctag aacagcaacg gtttcttttg aaattggggt
    12541 tgctccaatt gtagggcata gtccgattgc tcttttgtgt tttacagcct gtttttttgc
    12601 tgcagcttat tatctcgcta ttagaccaaa tggtatatta gatagtgttg gaaaaatctt
    12661 aacgcctgtt tttgcctttt taattctctc tttggttgtt gttggagcta ttgcttatgg
    12721 aaacttggag agtgctaagg cgagtgctga ctatgcagga aaagcgtttg gaagtggtgt
    12781 cttagcaggc tataatacac ttgatgcact tgcagcagtt gctttttgtc tagtagcaac
    12841 agaaacgctt aaaaagtttg gttttaaaac taaaaaagaa tatttatcaa ccatttggat
    12901 tgttggaatt gttactagtt tagcatttag tattttatat attggcttag gttttttagg
    12961 gaataagttt cctgtcccag ctgatatttt agctgaccca aatgttaata aaggagctta
    13021 tgtattatca caagcatctt ataaattatt tggaaatttt ggtcgttatt tcttgagtat
    13081 tatggtaaca ttgacatgct ttacaacaac agttgggctt attgtgtcag tatctgaatt
    13141 ttttgataag aactttcgtt ttgggaatta taaattattt gctactgttt ttactttgat
    13201 tggcttttta attgctaatc ttggtttaaa tgctgtaatt actttttcag ttcctgtgct
    13261 aactttattg tatccgattg ttattgttat tgtcttaatt attttgatta ataagtggtt
    13321 gccgctttca aaaaagggaa tgtctttgac aattggtctt gtcactttag tgtcgtttgt
    13381 tgaggttcta gcgggacaat ggcaagaaaa aacattaact cagttagttg gattcctccc
    13441 ctttcatact atttcaatgg ggtggctagt tccaatgttg attggtatag tattttcttt
    13501 ggtgctgtca gataagcaaa aaggccaagc atttgattta gagaaatttg aaggatagca
    13561 ctatttcaat tttatagata tgtcaaaaag gttaaaactt agttttagcc
//
LOCUS       DBJYSH010000054        13591 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.054, whole genome shotgun sequence.
ACCESSION   DBJYSH010000054 DBJYSH010000000
VERSION     DBJYSH010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 13591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13591
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.054"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            complement(<1..1633)
                     /locus_tag="ACUBV3_001201"
     CDS             complement(<1..1633)
                     /locus_tag="ACUBV3_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_1767 family glycosyltransferase"
                     /protein_id="HHU3847705.1"
                     /translation="MKELEEIVVFPINKSLDYILENHSSVIRFGDGEFDIIMGKSIPY
                     QDYNPELADRLLQLLHKQSTNDLLVCLPDMFQNRQRYNQSAQLFWKNHFQKFGNFYRQ
                     HCYQDWYGSSFISRPYMDLQDKESAVESFKKLKLLWDKRDILVVEGETSRSGVGNDLF
                     NNCNSLKRIICPSKNAYIKYDEILEAVERYAQGRIILLMLGPTAKILVRDLSDRGFQA
                     IDIGHIVSEYEWYKMGATQKIKFSHKHTAEFNDDENINLIDSQNYIDEIVKTITIDDT
                     EFRDTELISIILPVYNMSGSLSRCLDSILRQVYSTFEVILVNHGSTDGSGNICEKYAM
                     KDERIRLFHEEHQVITSVWNFALEKSKGKYVTFVNPDDFLDESYLNYLYNKLRANKSD
                     ISATTYTFFNEEEKCFVYFATDDNYFEKTFFAQEWLEQEHSYHDNLKQIFPLLSMKLF
                     RKSLFNNITFPLEDLTGEYTLYNVYLLSRHITFSHIGLYITSQVFSHTEIKRFTGHDI
                     EKMLSRKEERLALLTMVGFNSEKYIKNYKETLLNYQ"
     gene            complement(1641..2636)
                     /locus_tag="ACUBV3_001202"
     CDS             complement(1641..2636)
                     /locus_tag="ACUBV3_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HHU3847706.1"
                     /translation="MRTYITNLNGHSITSTAQIAQNMVTDIAVSLGFRELGIHSYPID
                     TDSPEEMSKRLDGICSGLRKNDIVIFQTPTWNTTTFDEKLFHKLKIFGVKIVIFIHDV
                     VPLMFDGNFYLMDRTIAYYNEADVLIAPSQAMVDKLQSYGLTVKKILVQGMWDHPTNI
                     TLQAVNHKKLVHFPGNPERFNFIKNWRIPTELHVYTDHNMQLPTTVVKEPYQSDEQLI
                     MKMSEGGYGLVWMDDRDKQYQSLYCPYKLGAYIAAGIPVIIQKGIANQDIIEKNNLGF
                     IIEKIDDISNIVESTTEEEYMEIVSDVRRFNPLVRQGYFTRKLLTDAVFSALNSM"
     gene            complement(2690..2857)
                     /locus_tag="ACUBV3_001203"
     CDS             complement(2690..2857)
                     /locus_tag="ACUBV3_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017284851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3847707.1"
                     /translation="MPKRNELLNKEIKMSIDKLRYKEPESEHDKRPTFYLVVLILVTV
                     AVILSLFKYFL"
     gene            complement(2850..4178)
                     /gene="gtfB"
                     /locus_tag="ACUBV3_001204"
     CDS             complement(2850..4178)
                     /gene="gtfB"
                     /locus_tag="ACUBV3_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000584700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031647 - regulation of protein stability
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosylation chaperone
                     GtfB"
                     /protein_id="HHU3847708.1"
                     /translation="MIILFDFFDKKSKDLYYSLITSGLHGNAVVINDDGFLPQNINSP
                     YSFFCNMEGKNGNPLYFNQVPLPDLWEIKGNNIEAEIWDFSIKRAKIFYQEPKYKRQV
                     KNIDWFDNNKKVRYTDHYNRFGWCFARTHFDKNQNVTTKSYFDKDGKEVIVENFRTGV
                     IILNWLNKDYFFDNRVAFLNFYFSLMGWNLSRIWYNSLSTPFFVSYRMTYPGEDILFW
                     QEDIEDTIPANMRVLLESTNTRTQKVIVQKKNTYHKIKSMLPKEQQEKIGYLGFIYPN
                     KKNNKGRKDIFILTNSDQIEHLEVLVHHLSDYHFHIAAYTEMSFKLMSFSQEQNVTLY
                     PNISRTDLDNLFEICDIYFDINHGNEVDDVIRRAFEYNHLIFAFDNTCHNRELVLDSN
                     IISHTTCEQLINLMKNLSGSIMYLLEQQREQTSNETKERYKEILGGYGNA"
     gene            complement(4162..5682)
                     /gene="gtfA"
                     /locus_tag="ACUBV3_001205"
     CDS             complement(4162..5682)
                     /gene="gtfA"
                     /locus_tag="ACUBV3_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosyltransferase GtfA"
                     /protein_id="HHU3847709.1"
                     /translation="MVVYNLNRGIGWASSGVEYAQAYRSEVFRKLGVEAKFIFTDMFQ
                     NENIEHLTRNIGFEDNEIIWLYTFFTDLTIAATSYSLQQLKESFSLPIDRTEKNGKII
                     SFFFKGSSIVVTVMLNDESSNIVQRVEYLMGGKLVRKDYYSYTKMFSEYYAPEDIGPC
                     LYQRTFYNEDGSVAYEENVDGENSIFKFKETILYSKEELVGYMLEKLQLTNSDLILLD
                     RSTGIGQAVLRNKGNAKVAVVVHAEHYNVSATDETTILWNNYYDYQFSNADSIDAFIT
                     STETQTKTLIDQFKKYLNIEPVVYTIPVGSLSKLQRKEWHERKAFSLLTCSRLASEKH
                     IDWLINAVVEANKVIPELTFDIYGEGGERQKLQEIIAKNKANNYIRLMGHKNLSSVYK
                     DYQVYLSGSTSEGFGLTLMEAIGSGLPIIGLDVPYGNQTFIENNLNGYLIPRETPDNP
                     QQISTAFAQYIVALFNSKDICKKHEYSYRIASRFLNDKIIENWSFFLRRLLNDYTI"
     gene            complement(5698..8094)
                     /gene="secA2"
                     /locus_tag="ACUBV3_001206"
     CDS             complement(5698..8094)
                     /gene="secA2"
                     /locus_tag="ACUBV3_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000758330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system translocase SecA2"
                     /protein_id="HHU3847710.1"
                     /translation="MKKVVVKPKISILNKLRLQRIQKIVNEINNKKAYYAQLSDKELS
                     HQTLILKNRLSSGESLDDILVDAFAVIREANKRILGLFPYDAQVMGAIALHQGTIAEM
                     KTGEGKTLTATMPLYLNALTSKGAILVTTNDYLAKRDALEMGKVYQFLGMTVGINVFD
                     KKEEADATIKREVYESDITYTTAGALGFDYLVHNLASNKLEQFLRPFHFVIVDEADSV
                     LLDIAQTPLIIAGDPRVQSNLYGITNNFVLTLKENDEYIHKAKDKIVYLTEKGVSYAK
                     QYFNISELYNDEYWELNRHINLALRAHCLYKRDYDYVVKNNEVKLLDNATGRVMEGTK
                     LQSGIHQAIEAKEDVALTNESRAIASITYQSLFNMFPRLSGMTGTGKSSEDELIKTYH
                     LPVIVIPTNFPIKRVDLADKIYVSLPEKLQATIDDVKQRHSKGQPVLLISGTVEIANI
                     YSKLLLREGIAHNTLTADNVVKEAQIIKEAGQKGSVTCATVLAGRGTDIKLGEGVREL
                     GGLAVIGTERMPNLRMDWQLRGRSGRQGDPGISQFYVSLEDELIVSHSPEWVIKYLKK
                     YSRKETSNYYMVPRKKRFFYQIVKNAQLRSEDKGVSSREQTIKFGESLRIQRENIYQL
                     RNELIADSSIVVDGVIKIIQDNFNDIANDKDLTEHSLRRYILENLTYKFKYFPDEFDV
                     HNSDDVFKLLIDIFDREFTAKKTKLQSDNEFDNFVRISILKAIDKSWIEEVDSLQQLK
                     GVVTNRGIGQRDIIQEYYKESLNSYYKMGKEIRYSIVKNIMLSTISKASDGSYSIYYV
                     "
     gene            complement(8091..8636)
                     /gene="asp3"
                     /locus_tag="ACUBV3_001207"
     CDS             complement(8091..8636)
                     /gene="asp3"
                     /locus_tag="ACUBV3_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp3"
                     /protein_id="HHU3847711.1"
                     /translation="MIKKRIQKVLWTDSSNVYLYGSTIKYLEHNRVFFENLRFSTGKT
                     IKKWLSKTNFQANRTLPELTLLKPNHTYELVSKLTTVPEGCVIIQINFYNRQKEKIGF
                     SIIRDHSSDFIVPDDTYFYDISLIGAGCISVEFCYLELFEVIETQEEKDGIELNSYVE
                     SSLPSELELIRTLIAFSKDKK"
     gene            complement(8629..10218)
                     /gene="asp2"
                     /locus_tag="ACUBV3_001208"
     CDS             complement(8629..10218)
                     /gene="asp2"
                     /locus_tag="ACUBV3_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp2"
                     /protein_id="HHU3847712.1"
                     /translation="MEKLKILQIGEEPWDADLITPDNIEWLYCNGNAIQIFLEKLKSK
                     ELEKNNVEYIGKTSTNEVKLWFNAVILTSEVSELQLNLLSDCLEAHTLFYDEDFQLDF
                     CSENGIFKRKVLRPLPFNGSRKEKIIFLSRTLFSEQYGAKLKVSDIDLNPDFKGEIIY
                     EGNSSICFSGNFGEDFQPLFSFRYNLSSTDKAIEIWQEYQKLQGDNSIMIEVIQYQKG
                     SLDLILNTKYLSENDLADPYILEYDESVGFSSISIFARGDGVVRFGALHWRYSRKGLG
                     QFLIGGERYSDSKRQEFIYYFNPGDMKPPLNVYFSGFRGAEGFEGFYMMKRLGAPFLL
                     IGDPRIEGGSFYLGTSEYESELENVLQKYLDYLQFDHCQLILSGLSMGSFGALYYGST
                     FSPHAIVVGKPFTNLGDMVVNLKLRRPDDFETSMDILQNIIGSQNDEAINQFNQKFWN
                     KFKRSNFEKTQFAISYMKHDDYDRNATPRLIDYFSKTNTLLYASGYAGRHNDNSQSIN
                     NWFTSQYKTILSDDFGRVFSD"
     gene            complement(10220..11797)
                     /gene="asp1"
                     /locus_tag="ACUBV3_001209"
     CDS             complement(10220..11797)
                     /gene="asp1"
                     /locus_tag="ACUBV3_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp1"
                     /protein_id="HHU3847713.1"
                     /translation="MFYFIPSWYNSDQKWHNEAPIWYRVLENMTFDDTVSQVRMFQNA
                     DEEVRLLLFSYNPQLRYMLHEESMLAVSYWSFFDDIQNIQQKEIKLINFKELNWPKGT
                     YFVYTPFLVLAKHGGEIIARIQFAENGNLLLIELLIDGVLDKIMIFDDRGFVSSIKYF
                     KQNRLIYQDYLNQNGVWQIREYASKFPRIVVNPMSDKVFNFTEYDDWDTLIRERILVL
                     KKLEVTPDDYIIVAVNDIHNNLVRKSFPDQKKIFSFFGERYSFDNRLDFNELLDEGEL
                     FIAESEKIERLLITSLQEKNYSSNYVENHISRIPPFDTRLRLGQSQNIEELIAYLLVD
                     DLSQTYLSEVLDQLLAKMEVNPSLELKIVSYKNDYDMTSLKEQVKDKILSSKNPDYFF
                     TSLSLEDENHFKEEQKTKIRGISFDIYTNENQIIDALDTARLVIDLRDDPDLYTQIAA
                     LSSGIPQINKVHTNYVEHQKNGWIISDSTELNNAIDYYFDGLMNWNKSLVYSVKKMGD
                     YTSGKILQRWRKLLEKE"
     gene            complement(11807..13060)
                     /gene="secY2"
                     /locus_tag="ACUBV3_001210"
     CDS             complement(11807..13060)
                     /gene="secY2"
                     /locus_tag="ACUBV3_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein translocase subunit
                     SecY2"
                     /protein_id="HHU3847714.1"
                     /translation="MIGKLYYSYRKSTLLRSILWTILIVGVYMLGQRVLLSTVPLSHQ
                     EIKLAVDQHLLNNFSAVSGGSFNKLNVFTLGLSPWMSSMIIWRFVSLFSWAKNATKRK
                     AEVAQYTLMLTISVIQAYGVSGNQFIKSSLLGSYSDIVFKALTMVLLISGSFVLMWLA
                     NLNASKGLGGSIVIILVSMLQATTTGIITYFEQSHFSIMDLLGTIIAISIILSVLLLI
                     STVVYRAEYRIPIRRIDIVSKFAQDTYIPIRINPAGSYPFMYGMTLMMLPTFIIQALL
                     LAFPNERLLIQISSMIAINKPLGIGLFVILLFILSIGFAYFNYDPYNIAKNMRQRGEY
                     FQNVTPGAATQRFLQEKISIMAVVGAIFTVIMGGLPMLAVVGKTNQVSIAMIVINIYI
                     ISTFMLSIIEQVNTLKLWGSHRNII"
     gene            complement(13169..>13591)
                     /locus_tag="ACUBV3_001211"
     CDS             complement(13169..>13591)
                     /locus_tag="ACUBV3_001211"
                     /inference="COORDINATES: protein motif:HMM:TIGR01167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HHU3847715.1"
                     /translation="ESVSNSESILSSESVSSSESISSSESVSNSESISTSESVSDSES
                     ISSSESISSSESVSMSTTESLSESEVSGDSEISSSTESSSQSESMNHTEIKSDSESQH
                     EVKHQVLPETGDNSASALGLLGAGLLLGATKSRKKKKD"
BASE COUNT         4515 a         2520 c         1810 g         4746 t
ORIGIN      
        1 tttgataatt taataaagtt tccttataat ttttgatgta tttctcagaa ttaaacccta
       61 ccattgttaa cagggctaac ctctcttcct ttctagatag catcttctct atatcatgtc
      121 ccgtgaatct ttttatttca gtatgagaga aaacttgaga ggttatgtat aaaccaatat
      181 gagaaaatgt aatgtgcctt gatagcaaat aaacattata cagtgtgtat tctcctgtta
      241 aatcttctaa aggaaaagtg atattgttaa aaagtgattt tctaaagagc ttcatagaaa
      301 ggagaggaaa tatttgtttt aaattatcgt ggtaactatg ttcttgctct aaccattctt
      361 gagcaaagaa tgttttctca aaataattat catctgttgc aaaataaaca aagcattttt
      421 cctcttcatt aaaaaaagta taggtggttg ctgagatatc agatttatta gctcttaatt
      481 tattgtaaag ataatttaag taagactcat caagaaaatc atctggattc acaaaagtaa
      541 catacttccc tttagatttc tctaatgcga agttccatac agatgtaata acttgatgtt
      601 cttcatgaaa taaacgaata cgctcatcct tcatcgcata tttctcgcaa atattaccac
      661 ttccatctgt agaaccatgg ttgactaaga tcacctcaaa agttgagtaa acttgcctaa
      721 ggatagaatc taaacacctt gataaagaac cagacatatt atagacagga agtatgattg
      781 atattaattc agtatccctg aattcagtat catcgatagt tattgtttta acaatctcat
      841 caatataatt ctgactgtca attaaattaa tattttcatc atcgttaaac tcagcggtgt
      901 gtttatgaga aaattttatt ttttgagtag ctcccatctt ataccattca tattcagaaa
      961 caatatgacc gatatcaatg gcttgaaatc ctctatcaga taaatctctt actaaaattt
     1021 ttgcagtcgg acctagcatc aataagatta tcctcccttg agcatatctt tcaacagcct
     1081 caagaatttc atcatatttt atataagcat ttttagaagg acaaataatc ctttttaaac
     1141 tattgcaatt attaaaaaga tcattaccta cacccgaccg cgaagtttcc ccttcaacta
     1201 ccaaaatatc gcgtttgtcc caaagtaact ttaatttttt aaatgattct acagcagact
     1261 ccttatcttg taagtccata tatggccttg aaataaaaga agaaccatac caatcttgat
     1321 aacaatgttg cctatagaaa ttgccaaatt tttgaaaatg atttttccaa aaaagttgtg
     1381 ccgattgatt atatctttgt cgattttgaa acatatctgg aagacatact aatagatcat
     1441 tcgtactttg cttatgaaga agttgcaata acctatctgc taactctggg ttgtaatctt
     1501 gataaggaat cgattttccc attataatgt caaattcacc gtccccaaag cgaataactg
     1561 acgaatgatt ttccaaaatg taatccaaac ttttgtttat agggaaaact acgatttctt
     1621 ctagttcttt catattcccc tcacatgcta tttaatgcac taaatactgc atcggttaat
     1681 agttttcgcg taaaatatcc ttgtctaact aaaggattaa accgcctgac atcgctaaca
     1741 atttccatat attcctcttc agtagtactt tcaactatat tactaatatc atcaattttt
     1801 tcaataataa atcccaaatt atttttttct attatatctt gattagcaat acctttttga
     1861 ataatcactg gaattcctgc tgctatataa gcgcccagtt tgtaagggca ataaagagat
     1921 tgatattgtt tatcacggtc atccatccag actaagccat atcccccttc gctcattttc
     1981 ataattaatt gttcgtccga ttgatatggc tctttaacca cagtggtggg taattgcatg
     2041 ttatggtcag tataaacatg taactcggta ggaattctcc aattctttat gaagttaaac
     2101 cgctctgggt tcccaggaaa atgaacaagc ttcttatgat taactgcttg taaagtaata
     2161 ttggtgggat gatcccacat tccttgaact aagatttttt ttactgtcaa gccataactt
     2221 tgaagcttat cgaccattgc ttgactaggg gctattaaaa catctgcttc attataataa
     2281 gctatagttc tatccatcaa ataaaaattt ccatcaaaca ttagcggtac aacatcatgt
     2341 ataaaaataa caatctttac accaaatatt tttaatttgt gaaataattt ttcatcaaaa
     2401 gttgtagtgt tccatgtagg tgtctgaaat atgacaatat cattttttct aagtccggaa
     2461 cagattccat ctaaacgctt actcatttcc tcaggagaat cagtatcaat cggataagaa
     2521 tgtattccca gctcacgaaa acctaagctt actgctatat ctgttaccat gttttgagct
     2581 atttgtgctg tactagtgat tgaatgtcca ttcaagtttg taatatatgt cctcacacac
     2641 ttcccctatc taaaaaacaa caggcttctt ttctaccatt aataggaatc tataaaaaat
     2701 atttaaataa cgacaatata actgctacag taacaagtat aagtactacc aaataaaaag
     2761 taggtcgctt gtcatgttca ctctctggtt ctttatatct aagtttatca atactcattt
     2821 taatttcttt attgagtaat tcatttcttt taggcatttc cataccctcc taatatttct
     2881 ttataacgct cttttgtttc attacttgtt tgttctcttt gttgctctag caaatacata
     2941 atggagcctg ataaattttt cattaaattt atcaattgtt cacaggttgt gtgagaaatg
     3001 atattgctat ctaatactaa ctctctgtta tgacaggtat tatcaaaagc aaagataagg
     3061 tgattatatt caaatgccct tctaataaca tcatcaactt catttccatg attaatgtca
     3121 aaataaatat cacagatttc aaataagttg tctaaatcag ttcttgaaat attcggatat
     3181 agcgttacat tttgttcttg gctaaatgac atcaacttaa aagacatttc tgtgtacgct
     3241 gcaatatgaa aatgataatc agacaagtga tgaaccaaca cttctaaatg ttcaatttgg
     3301 tcagagtttg tcaaaataaa aatatctttc ctacccttat tgttcttttt attaggatat
     3361 ataaagccta aataaccaat tttttcttgt tgttctttag gtaacattga ctttatttta
     3421 tgatatgtat ttttcttttg aacaattact ttctgagttc tagtattcgt actttctaat
     3481 aacacgcgca tattagcagg aatagtatcc tctatatctt cttgccaaaa taaaatatct
     3541 tctcctggat aagtcatccg gtaagaaacg aaaaatggag ttgataagga gttgtaccat
     3601 attcgagaca agttccaacc cataaggcta aaataaaaat ttaagaatgc aactcgatta
     3661 tcaaaaaaat aatctttatt tagccaatta aggattataa ctcctgttct aaaattttca
     3721 acaataactt ctttaccatc cttatcgaaa taagattttg tagtcacatt ttgattctta
     3781 tcaaaatgtg tacgtgcaaa acaccatccg aagcgattat aatgatctgt gtatctcact
     3841 tttttattat tgtcaaacca atcaatattt tttacttgac gtttatactt cggctcttga
     3901 taaaaaattt tagctctttt aatactaaaa tcccaaattt cagcctctat attatttccc
     3961 tttatctccc aaaggtcagg gagtgggact tggttaaaat aaagaggatt cccatttttc
     4021 ccttccatat tacaaaaaaa tgaataagga gaatttatat tttgtggaag aaaaccatcg
     4081 tcattaatga ccacagcatt tccatgtaaa cctgaagtaa ttaaagaata atatagatct
     4141 ttgcttttct tatcaaagaa atcaaatagt ataatcattt aataacctcc tcaaaaaaaa
     4201 actccaattt tctataattt tatcatttaa aaatctacta gctattctat aagaatactc
     4261 gtgtttttta caaatatctt tagaattaaa taaagcaaca atatactgag caaaagcagt
     4321 tgatatttgt tgaggattat ctggtgtttc ccttggaata aggtagccat tcaaattatt
     4381 ttcaataaag gtctgattac catacggaac atctaaacca attatcggaa gccccgatcc
     4441 gattgcctcc atcaaggtta aaccaaaacc ttcactggta gaacccgata aataaacttg
     4501 ataatcttta taaacagaag ataggttttt atgccccatt aatcgtatat aattatttgc
     4561 cttattttta gcaattattt cttgtaactt ttgccgttcg ccaccttcac cataaatatc
     4621 aaaggttaat tcaggaatta ctttattagc ttctaccaca gcattaatta accaatcaat
     4681 atgtttttcc gaagctagac gtgagcaagt taacaacgaa aaagcttttc tttcatgcca
     4741 ctcttttctc tgtaattttg ataaacttcc tacaggaatt gtataaacga ccggttcaat
     4801 atttaaatac tttttaaatt gatcaatcaa cgtcttagtt tgcgtttctg ttgaggtgat
     4861 aaaagcatct atgctatcag catttgaaaa ttgatagtca taataattat tccataaaat
     4921 cgtagtttca tcagttgcgc taacattata atgctctgca tgaacaacaa cagctacttt
     4981 agcatttcct ttattcctta gaacagcttg cccaatgcct gtagagcggt ctaacaagat
     5041 tagatcacta ttcgttaatt gtaatttttc taacatgtat ccaactaatt cttctttaga
     5101 atatagtata gtttctttga atttaaaaat actattctcg ccatctacat tttcttcata
     5161 tgcaacactt ccatcttcat tataaaatgt acgctgatac aggcatggac ctatatcttc
     5221 gggagcataa tattctgaaa acattttagt atatgagtag tagtcttttc taacaagctt
     5281 tccccccata agatactcta ccctttggac gatgttacta gattcatcgt taagcataac
     5341 tgtaactaca atactacttc ctttaaaaaa aaatgatatt attttaccgt ttttctcagt
     5401 cctatcaatc ggtagtgaaa aactttcttt caattgttgc agtgaataag aagttgcagc
     5461 aattgttaaa tcagtaaaaa aagtataaag ccatataatc tcattgtctt caaagccgat
     5521 atttcgagta aggtgctcta tattttcatt ctgaaacata tcagtaaaaa taaatttagc
     5581 ttcaacacct aactttctaa aaacttctga acgatatgct tgagcatact ctacaccgct
     5641 acttgcccaa cctatccctc tattcaaatt ataaactacc attaactccg ttttccttta
     5701 tacataatat attgagtaac ttccatcact tgcttttgaa attgtactta acataatatt
     5761 cttaacgata gaatatctta tttcttttcc catcttatag taagaattaa gcgattcttt
     5821 ataatattct tgaataatat ctcgttgacc gatacctcta ttggttacaa ctccctttaa
     5881 ttgctgtagg ctatccactt cttctatcca cgatttgtca atagccttta atatcgatat
     5941 tctgacgaaa ttatcaaatt cattatcact ttgaagttta gttttctttg cagtaaattc
     6001 tctatcaaat atgtcaatca ataatttaaa tacatcgtca gaattatgaa cgtcaaattc
     6061 atcagggaaa tatttaaatt tataggtaag attttctaat atatatcttc gtaaagaatg
     6121 ttccgttaaa tccttatcat tagcaatatc gttaaaatta tcctgaataa tttttatcac
     6181 accatcaacc acaatggaac tatcagctat taattcattt cgtaattgat aaatattttc
     6241 acgctgtata cgcaagctct ctccgaactt aatagtttgc tccctagaag atacaccttt
     6301 atcttcactt ctgagttgag catttttaac tatttgataa aaaaagcgtt tctttctagg
     6361 taccatatag taatttgaag tttctttacg agaatatttt tttaaatact ttatcaccca
     6421 ttccggagaa tgagaaacaa ttaattcatc ctctagcgac acataaaatt ggctaatccc
     6481 tgggtctcct tgacgccccg aacggcccct aagctgccag tccatacgta aattaggcat
     6541 cctttccgtt cctataactg caagtcctcc taattctcta accccttcac ctagtttaat
     6601 atctgttcca cgaccagcaa gaactgttgc acatgttact gaaccttttt gtcccgcttc
     6661 ttttataatt tgtgcctctt taacaacatt atcagctgtt aaggtattat gggcaatgcc
     6721 ttctcgcagc aataactttg aataaatgtt agcaatttca actgttccag aaattaacaa
     6781 tactggctga cctttactat gtctttgttt aacatcatct atagtagctt gcaacttttc
     6841 tggtaagcta acatatattt tatcggcaag atctactcgt ttgatcggaa aatttgttgg
     6901 tataacaata acaggcaggt ggtaagtttt tataagttca tcttcagaag attttccagt
     6961 tcctgtcatc ccagacaacc ttgggaacat attaaagaga ctttgatacg ttattgaagc
     7021 aattgcccta gattcatttg tcaaagcaac atcttctttt gcttcaatag cttggtgaat
     7081 ccctgattga agttttgtgc cttccataac acgacctgtt gcgttatcta acaattttac
     7141 ttcattattt ttaactacat aatcataatc tcttttatac aaacaatgag cacgcaatgc
     7201 taggtttata tgtctattta attcccaata ctcatcatta tataattctg aaatattgaa
     7261 gtactgtttt gcgtaactta cacccttctc agtaagatag acaattttat ccttagcttt
     7321 atggatatat tcatcatttt ccttaagagt gagtacaaaa ttattagtga taccatagag
     7381 attagattgt acacgaggat ctcccgctat aattaagggt gtttgagcaa tatctaacaa
     7441 aacagagtct gcttcatcaa cgataacaaa atgaaaaggt cttagaaatt gttctagttt
     7501 attactagcc agattgtgaa cgagatagtc gaaacctaag gcacctgcag tagtataagt
     7561 aatatcagat tcatatactt ctctcttaat agtcgcatct gcttcttctt ttttatcaaa
     7621 aacattaata ccaactgtca tacctagaaa ttgatatact tttcccatct ctaatgcatc
     7681 tcgttttgcc aaataatcat tggtcgtcac taagattgct cccttgctag ttaaagcatt
     7741 cagatacaag ggcatagttg cagttaatgt ttttccttct cccgttttca tttcagctat
     7801 cgttccttga tgaagagcaa tagcacccat aacctgagca tcatatggaa acaaacctaa
     7861 aattctttta ttagcctctc ttatcacagc aaaagcatca acaagaatat catctagact
     7921 ttctccactt gaaagtctat tttttaatat taaagtttga tggcttaact ccttatctga
     7981 taattgagcg tagtacgctt ttttattatt tatctcgttt acaatttttt gaattctttg
     8041 caatctcaat ttatttagaa tagagatttt gggtttaaca acaacttttt tcatttttta
     8101 tccttagaaa atgctatcaa cgttctaata agttcaagct ctgatggtaa gctgctttca
     8161 acataactgt ttagttcaat accatctttt tcttcttgag tctcaattac ttcaaataat
     8221 tctaggtaac aaaattctac acttatacaa cccgcaccaa taagagaaat atcataaaaa
     8281 tatgtgtcat caggaactat aaaatctgag gagtgatctc gaataataga aaaacctatt
     8341 ttttcttttt gacgattata aaaatttatc tgaataatca cgcatccctc aggcacagta
     8401 gttaattttg ataccaactc ataggtatga ttaggtttta gtaaggtaag ctctggcaac
     8461 gttctattag cttgaaaatt cgttttagaa agccattttt tgattgtttt tcctgtagag
     8521 aatcttagat tctcaaaaaa aacacgatta tgctccaaat actttatagt cgaaccataa
     8581 agataaacat tgctactatc agtccaaagc accttctgta ttcttttttt aatcactaaa
     8641 cactctccca aaatcatcac tgagaattgt tttatactga gaagtaaacc aattatttat
     8701 tgattgacta ttatcattat gtcgcccagc atatccactt gcatacaaca gggtgttagt
     8761 tttagaaaaa taatcaatca gccttggagt tgcatttcta tcataatcat catgtttcat
     8821 ataagatata gcaaactgcg ttttttcaaa attagaacgc ttaaacttat tccaaaattt
     8881 ttgattaaat tgattaatag cttcatcgtt ttgagaacca ataatatttt ggagaatatc
     8941 catactcgtt tcaaaatcat ctggtcttct taattttaaa tttactacca tatcgcctaa
     9001 atttgtaaag ggtttcccca caactatagc atggggggaa aaagtagaac cataataaag
     9061 ggcaccaaaa cttcccatag ataaacctga taaaataagt tgacaatgat caaattgtag
     9121 gtaatctaaa tatttttgta aaacattttc taattcactt tcgtattctg aagtacctag
     9181 gtagaagctt ccaccttcaa tccttggatc accaattaag agaaacggag ctcccaaacg
     9241 tttcatcatg taaaaaccct caaaaccttc ggctcctcga aaacctgaaa aataaacatt
     9301 caatggaggt ttcatatctc cagggttaaa atagtagata aactcttgcc ttttggaatc
     9361 actgtacctt tctccaccga ttaaaaattg acccaaacct ttacgcgaat atcgccaatg
     9421 tagtgcacca aatctcacca ctccgtcacc tctagcaaag atagaaattg atgaaaaacc
     9481 aactgattca tcatattcta aaatatatgg atcagccaaa tcattttctg aaaggtattt
     9541 tgtatttaat atcaaatcaa gtgatccttt ttggtattga atgacttcta tcataatact
     9601 attatcacct tgcaattttt gatattcttg ccatatttct atcgctttat cagtagagga
     9661 taaattatag cgaaatgaga atagaggttg aaaatcttct ccaaaattcc ctgaaaaaca
     9721 aatagagcta ttgccttcat atattatctc tcccttaaaa tcaggattta gatctatgtc
     9781 agaaaccttc aattttgcac catattgttc cgaaaaaaga gtacggctta ggaaaataat
     9841 tttttctttc cttgatccat taaatggtag tggtctcaaa accttacgtt taaatatacc
     9901 gttttctgag caaaaatcga gttgaaaatc ctcgtcgtaa aagagtgtat gcgcttctaa
     9961 gcaatctgaa agtaggttca attgcaattc gctaacctca ctagttaaaa tgaccgcatt
    10021 aaaccataat ttaacttcat tagttgaagt tttaccaata tattcaacat tatttttttc
    10081 taactcttta ctttttaatt tctctaagaa aatttgaata gcatttccat tacagtataa
    10141 ccattcaata ttgtctggag ttattaaatc agcatcccat ggttcctcac caatctgcaa
    10201 aatttttaat ttttccatat tattcttttt ctaataattt tcgccagcgc tgtaatatct
    10261 taccgcttgt ataatcaccc atttttttta cgctgtaaac taacgattta ttccaattca
    10321 ttagtccatc aaaataatag tcaattgcat tattgagttc tgtagagtca ctaataatcc
    10381 agccattctt ctggtgttct acataattag tgtgaacttt attgatttga ggaattccag
    10441 aactcagagc agcaatttga gtatataggt ctggatcatc tcttaaatca ataactaacc
    10501 tagcagtatc tagagcatct ataatttgat tttcatttgt atatatatca aaactaatgc
    10561 cacgaatttt agttttttgt tcttccttaa aatgattctc atcttccaaa gaaagagagg
    10621 taaaaaaata atctggattt ttagaagaca atattttgtc cttaacttgt tcttttagag
    10681 aagtcatatc ataatcattt ttatagctga ctattttcaa ctctaatgac ggattaactt
    10741 ccatttttgc taataattgg tctaaaactt cacttaaata agtttgagaa agatcatcta
    10801 caagtaaata tgcaataagc tcttcaatat tttggctctg tccgagtctc aaacgagtat
    10861 caaaaggggg aatacggcta atgtgattct caacatagtt tgacgaataa tttttctctt
    10921 gtaatgaagt aatcaataac cgttctattt tttcgctttc agcaataaac aattcacctt
    10981 catcaagaag ttcattaaag tcgagtcgat tatcaaatga atagcgttca ccaaaaaatg
    11041 agaaaatttt cttttgatct ggaaatgatt ttctgactaa attgttatga atatcgttta
    11101 ctgctactat aatataatca tctggagtca cttctagttt ttttaaaacc aatattctct
    11161 ctcgaataag agtatcccaa tcatcatact cagtaaaatt aaacacctta tcagacattg
    11221 gattaactac tattctcgga aattttgaag catactctcg aatttgccaa acaccatttt
    11281 gatttaaata atcttgataa attaatctat tttgtttgaa atatttgata ctggatacaa
    11341 aaccacgatc gtcaaatatc ataatcttat caagcacacc atctattaaa agttcaatca
    11401 gtagtaaatt cccattttcc gcaaattgga tacgtgcaat aatctcgcca ccatgtttag
    11461 ctaatactaa aaaaggtgta taaacaaaat aagtcccttt aggccaattc agttctttaa
    11521 aattaattaa cttaatctct ttttgttgaa tattttgaat atcgtcaaaa aaagaccaat
    11581 aagagacagc caacatactc tcttcatgaa gcatgtatct caactgagga ttataactaa
    11641 atagaagtaa acgcacttcc tcatctgcat tctgaaacat tctaacctga ctaacagtat
    11701 catcaaaagt catgttctcc aatacacgat accagatagg agcttcatta tgccactttt
    11761 gatcagaatt gtaccacgaa ggaataaaat aaaacatgat tgctccttaa ataatatttc
    11821 ggtgactgcc ccacaacttc aaggtattaa cttgctcaat aatactgagc ataaaagttg
    11881 aaataatata aatattaatg acgatcattg caatactaac ttggtttgtc ttccccacca
    11941 cagccaacat tgggagacct cccataataa ccgtaaaaat tgctccaact actgccatga
    12001 tactaatttt ttcttgtaaa aaacgttgag tagcagctcc tggagttaca ttttgaaaat
    12061 attccccccg ttgacgcata ttttttgcaa tattatacgg gtcgtaatta aaatatgcaa
    12121 atccaatgct caaaataaat aataatataa caaaaagtcc aatacctaaa ggcttattaa
    12181 ttgcaatcat tgacgagatc tgaatcaata atcgctcgtt tggaaaagca agtaacaatg
    12241 cttgaattat aaacgttgga agcatcatta atgtcatccc atacatgaaa gggtaggacc
    12301 ctgctggatt tatgcgaata ggaatatagg tatcttgggc aaatttacta acgatatcaa
    12361 tacgcctaat aggtatacga tattctgccc tataaaccac cgttgagatt aataataaaa
    12421 cactaagtat tatacttata gctataatag ttcccagcag atccataata ctaaaatgtg
    12481 actgttcaaa ataagttatt attccagtag tcgttgcttg gagcatgctc actaatatga
    12541 taacaatact tcctcctagc cctttagaag cattgagatt agcaagccac attaacacaa
    12601 atgagccact tattaacaag accattgtca atgctttaaa aacaatatca ctataagaac
    12661 ctaataaaga gctttttata aattgatttc ctgaaacacc atatgcttgt ataactgaga
    12721 tagtaagcat taaagtatat tgagctactt ctgcttttcg cttcgttgca ttttttgccc
    12781 acgaaaataa ggaaacgaat ctccaaataa tcatacttga catccatgga ctcaacccca
    12841 gtgtgaaaac atttaattta ttaaaactcc caccacttac tgctgaaaag ttattgagta
    12901 aatgttgatc tactgctagt tttatctctt gatgtgataa aggaacagtg gataataaaa
    12961 cacgttgtcc taacatatat acaccaacaa ttaaaatagt ccataaaata cttcttaata
    13021 gcgttgactt tctatagcta taatataatt ttccaatcat ttttcccttt tcaaatcaat
    13081 ttatcactta agacagtata actacttact atcttaagtg ataaatcgaa agtactttcg
    13141 atttatcatc taaatatcac tttgaaaatt aatctttttt cttcttgcga gatttagtag
    13201 ctcctaacaa caatcctgca cctaacagac ctaatgctga agccgagtta tcacctgttt
    13261 ctggtaatac ttgatgctta acttcgtgtt gagattcgga atcggattta atttcagtat
    13321 gattcatcga ttcagattga cttgatgatt ctgtacttga gctaatttca gaatcccctg
    13381 atacttctga ttcacttaga gactctgtag tactcattga tactgactct gaacttgaaa
    13441 tagattcaga acttgaaata gactctgaat ctgataccga ttctgaagtt gaaatagact
    13501 ctgaatttga taccgattct gaacttgaaa tagattctga gcttgatact gattcggaac
    13561 ttaaaataga ctcggagttt gataccgatt c
//
LOCUS       DBJYSH010000055        13540 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.055, whole genome shotgun sequence.
ACCESSION   DBJYSH010000055 DBJYSH010000000
VERSION     DBJYSH010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 13540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13540
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.055"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            complement(24..1805)
                     /locus_tag="ACUBV3_001212"
     CDS             complement(24..1805)
                     /locus_tag="ACUBV3_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000942633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HHU3847716.1"
                     /translation="MLRALKEDFFLTLTSLKNQFWVYIGTGILIQTLVAYLVKGILSF
                     IFRRILILSNTPAVTKDNWTLLFNHPLALLLFILYIVILIGFIYTEFAIYTIAILKTE
                     FSLSIKKWLTIFPKKIKSLLGPQLIWVSIYLLLTIPLANLGLRSSILEHLKIPDFISG
                     ELTKTVFGKIGYSTLLLLICYLNLRLIYFLPLTILTDYNAKEALLESWKLSRGKRQWR
                     LLSKIILTSLFISVIGTVALAVVAGTFSFIDHSGNNFPLQATFYNLLKSILFAVTLLI
                     KCLIISHLLADIKQNKSIVQQWRAWKGVHHKQKFKHIACVILALAIGTTALKNAIALA
                     LLQDNISIKKELIAHRSDTSHGVENSIEALKAAHKAKADYSEMDVIMTKDHKLVVIHD
                     DNLKRLSGMNKDVSKLTLDQVTKIPIHQGHFASYIPSLAEFMKTAQSLDQKIMIELKP
                     YNQNLDIYADEFIKEFKELRLSTKHKVMSLNLTLIEKVEKKLPQLDTGYLIPLHWGTL
                     QNHNVDFYGIEEFSYNDWIAYLAQEYNKQLYVWTINRDNLMIRYLQSPVNGIITDKLN
                     LFKVINKDIKNSPNYYQRALQLIDSEG"
     gene            1975..2577
                     /locus_tag="ACUBV3_001213"
     CDS             1975..2577
                     /locus_tag="ACUBV3_001213"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HHU3847717.1"
                     /translation="MKFLELNKKRHAVKHFNDKPVDFKDVRTAIEIATLAPSANNIQP
                     WKFVVVQEKKSALAEGLPESNCNQINQAQYVIALFTDTDLGQRSRKIARIGRRSLPDD
                     LIGYYMETLPPRYALYSEKQTGEYLSLNAGIVAMNLVLALTDQGISSNMILGFDKAIT
                     NDVLEIDKRFRPEILITVGYSDEKVEPSYRLPVDHIIEKR"
     gene            2624..4030
                     /gene="pepV"
                     /locus_tag="ACUBV3_001214"
     CDS             2624..4030
                     /gene="pepV"
                     /locus_tag="ACUBV3_001214"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HHU3847718.1"
                     /translation="MTIDFRAEVDKRKDALMDDLINLLRINSERDDSQADAEHPFGPG
                     PVKALEFFLEMAERDGYETKNVDNYAGHFTFGQGEEELGIFGHLDVVPAGSGWDTDPY
                     EPVIKDNRLYARGSSDDKGPTMACYYALKIIKELGLPTSKKVRFVVGTDEESGWGDMD
                     YYFEHVGLPKPDFGFSPDAEFPIINGEKGNITEYLHFSGENKGAVRLHSFSGGLRENM
                     VPESATARFTSHLDQTTLGASLADFAGKHNLKAELSVEDGQYIATVYGKSAHGSIPQE
                     GVNGATYLALYLSQFDFEGPARAFLDVTANIIHEDFSGEKLGVAYEDDCMGSLSMNAG
                     VFQFDETNDDNTIALNFRYPQGTDAKTIQTKLEKLNGVEKVTLSDHEHTPHYVPMDDE
                     LVSTLLAVYEKQTGLKGHEQVIGGGTFGRLLERGVAYGAMFPGDENTMHQANEYMPLE
                     NIFRSAAIYAEAIYELIK"
     gene            4127..4711
                     /locus_tag="ACUBV3_001215"
     CDS             4127..4711
                     /locus_tag="ACUBV3_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000130595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HHU3847719.1"
                     /translation="MTDLEKIIKAIKSDSQNQNYTENGIDPLFAAPKTARINIVGQAP
                     GLKTQEAILYWKDKSGDRLRQWLGVDEETFYHSGKFAVLPLDFYYPGKGKSGDLPPRK
                     GFAEKWHPLILKEMPNVQLTLLVGQYAQKYYLGSSAHKNLTETVKSYKDYLPDYLPLV
                     HPSPRNQIWLKKNPWFEKDLIVDLQKIVADILKD"
     gene            4726..5460
                     /locus_tag="ACUBV3_001216"
     CDS             4726..5460
                     /locus_tag="ACUBV3_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HHU3847720.1"
                     /translation="MRDNHLHTHFSYDCQTAFEDYINGFTGEFITTEHFDLSNPYTGQ
                     DDVPDYSAYCQKIDYLNQKYGNRFKKGIEIGYFKDRESDILDYLKNKEFDLKLLSIHH
                     NGRYDYLQEEALKVPTKELLADYFNRMEFAIGRVEAHVLDHFDYGFRKLNLDVEDLKP
                     FETQLKRIFRKMLSKGLAFELNTKSLYLYGNEKLYRYALEILKQLGCKQYSIGSDGHI
                     PEHFCYEFDRLQCLLKDYQIDENHLI"
     gene            5629..9387
                     /locus_tag="ACUBV3_001217"
     CDS             5629..9387
                     /locus_tag="ACUBV3_001217"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pullulanase"
                     /protein_id="HHU3847721.1"
                     /translation="MKRKDLFGDKQTQYTIRKLSVGVASVATGVCIFLHSPQVFAEEV
                     SVSPATTAIAKSNINQVDNRQSTNLKDDINSNSETVVTPSDMPDTKQLVSDETDTQKG
                     VTEPDKATSLLEENKGPVSDKNTLDLKVAPSTLQNTPDKTSQAIGAPSPTLKVANQAP
                     QIENGYFRLHLKELPQGHPVESTGLWIWGDVDQPSSNWPNGAIPMTNAKKDDYGYYVD
                     FKLSEKQRKQISFLINNKAGTNLSGDHHIPLLRPEMNQVWIDEKYGIHTYQPLKEGYV
                     RINYLSSSGNYDHLSAWLFKDVATPSTTWPDGSNFVNQGLYGRYIDVPLKTNAKEIGF
                     LILDESKTGDAVKVQPNDYVFRDLANHNQIFVKDKDPKVYNNPYYIDQVQLKDAQQTD
                     LTSIQASFTTLDGVDKTEILKELKVTDKNQNAIQISDITLDTSKSLLIIKGDFNPKQG
                     HFNISYNGNNVTTRQSWEFKDQLYAYSGNLGAVLNQDGSKVEASLWSPSADSVTMIIY
                     DKDNQNRVVATTPLVKNNKGVWQTILDTKLGIKNYTGYYYLYEIKRGKDKVKILDPYA
                     KSLAEWDSNTVNDDIKTAKAAFVNPSQLGPQNLSFAKIANFKGRQDAVIYEAHVRDFT
                     SDQSLDGKLKNQLGTFAAFSEKLDYLQKLGVTHIQLLPVLSYFYVNEMDKSRSTAYTS
                     SDNNYNWGYDPQSYFALSGMYSEKPKDPSARIAELKQLIHDIHKRGMGVILDVVYNHT
                     AKTYLFEDIEPNYYHFMNEDGSPRESFGGGRLGTTHAMSRRVLVDSIKYLTSEFKVDG
                     FRFDMMGDHDAAAIELAYKEAKAINPNMIMIGEGWRTFQGDQGKPVKPADQDWMKSTD
                     TVGVFSDDIRNSLKSGFPNEGTPAFITGGPQSLQGIFKNIKAQPGNFEADSPGDVVQY
                     IAAHDNLTLHDVIAKSINKDPKVAEEDIHRRLRLGNVMILTSQGTAFIHSGQEYGRTK
                     RLLNPDYMTKVSDDKLPNKATLIEAVKEYPYFIHDSYDSSDAINHFDWAAATDNNKHP
                     ISTKTQAYTAGLITLRRSTDAFRKLSKAEIDREVSLITEVGQGDIKEKDLVIAYQTID
                     SKGDIYAVFVNADSKARNVLLGEKYKHLLKGQVIVDADQAGIKPISTPRGVHFEKDSL
                     LIDPLTAIVIKVGKVAPSPKEELQADYPKTQSFKGSKTVEKVNRIANKTSITPVVSNK
                     TDSYLTNEANLPKTGDKSSKILSVVGISILASLLALLGLSLKRNRHLK"
     gene            9522..10439
                     /locus_tag="ACUBV3_001218"
     CDS             9522..10439
                     /locus_tag="ACUBV3_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HHU3847722.1"
                     /translation="MKNWEKPPLVMAGLVLGLLALGNLLEGYGTYFRYCLGLVALVFW
                     IFLIKGILKNKKESRKELSNPLIASVFTTFFMAGMILSTYILLFRSLGIWVAVLSKGV
                     WWLSFIALIIHMAIFSWKYLRHFSMANLFPSWSVLYVGIGVASLTAPISGQFTIGKIV
                     FWYGFIATLVLLPFLFIKAYKIGLPSAVKPNITTICAPMSLITAGYVNSFVSPNRGLL
                     LLLIVMAQFLYFFILFQVPKLLIGDFTPGFSAFTFPLVISATSLKLSIQHLSLPVDIQ
                     GLVHFEIGTTTLIVMIVMVRYIFFLRRTI"
     gene            complement(10481..10657)
                     /locus_tag="ACUBV3_001219"
     CDS             complement(10481..10657)
                     /locus_tag="ACUBV3_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HHU3847723.1"
                     /translation="MVKKFAFAKGIATGVVATAATLAGAAFAIKKTIIEPEEEKIAFI
                     EENRKKAARKRVSH"
     gene            10768..11658
                     /gene="miaA"
                     /locus_tag="ACUBV3_001220"
     CDS             10768..11658
                     /gene="miaA"
                     /locus_tag="ACUBV3_001220"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HHU3847724.1"
                     /translation="MRKIKLIAVVGPTAVGKTALGIELAKTFNGEIISGDSQQVYQKL
                     DIGTAKASKEEQEQAYHHLIDVREVNENYSVYDFVKEAKVAIDTIISKGKIPIIVGGT
                     GLYLQSLFEGYHLGGEVNQETLKAYREKLESLSDEDLFEKLTEQSIVIPQVNRRRAIR
                     ALELAKFGNDLQNSESPYDVLLIGLNDDRQVLYDRINRRVDLMIDNGLLDEAKWLYDN
                     YPSVQASRGIGYKELFPYFSKQIPLEEAVDKLKQNTRRFAKRQLTWFRNRMNVEFIMV
                     GEENYQQKIKRKVSDFLSSK"
     gene            11749..12987
                     /gene="hflX"
                     /locus_tag="ACUBV3_001221"
     CDS             11749..12987
                     /gene="hflX"
                     /locus_tag="ACUBV3_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HHU3847725.1"
                     /translation="MIETKEEQERVILVGVELQDTENFEMSMEELASLAKTAGANVVN
                     HYYQKRDKYDSKSFIGSGKLEEIKAIVEADEIDTVVVNNRLTPRQNSNLEAELGVKVI
                     DRMQLILDIFAMRARSHEGKLQVHLAQLKYMLPRLVGQGIMLSRQAGGIGSRGPGESQ
                     LELNRRSIRHQISDIERQLKIVEKNRETVRERRVDSTTFKIGLIGYTNAGKSTIMNVL
                     TDDKQYEANELFATLDATTKQIYLQNQFQVTLTDTVGFIQDLPTELVAAFKSTLEESR
                     HVDLLLHVIDASDPNHEEHEKVVMEILKDLDMIDIPRLAIYNKMDVTEQLNATTFPNV
                     RIAAKKQGSRDLLRRLIVDEIRHIFDEFSIRVHQNQAYKLYDLNKIALLDTYTFEEEY
                     ENITGYISPKQKWKLEEFYD"
     gene            12980..>13540
                     /locus_tag="ACUBV3_001222"
     CDS             12980..>13540
                     /locus_tag="ACUBV3_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HHU3847726.1"
                     /translation="MIDYIDLALTYGGFTSLDKVYLEKKLDGLSKQQRLDFITPPPSV
                     INAYFAEIYQKQGPEAATDYYFDLSKALGLFPKHLSFDEEKPFIRLNLSGKSFGFAYL
                     NDQEEASVFSEVKEVITPQLLLEIAQIFPQYKVYQDRSGIRMAKIDFDETESQNITPE
                     TSLLGNVLQLKKDIIKITSFNQEELLE"
BASE COUNT         4578 a         2088 c         2601 g         4273 t
ORIGIN      
        1 atctactaag ctttttattt aatttaaccc tcgctatcaa taagttggag agctctttga
       61 taataattag ggctattttt gatatcttta ttaatgacct tgaaaagatt gagtttatct
      121 gtgatgattc cattaactgg gctttgtaaa taacgtatca taaggttatc acggttaata
      181 gtccaaacat agagctgttt attatattct tgggcgagat atgcaatcca atcattgtat
      241 gaaaattcct caatcccata aaagtcaacg ttatgatttt gaagcgttcc ccaatgtagt
      301 gggattaggt aacctgtatc aagttgtggc aattttttct caaccttttc aatgagtgtc
      361 aagttgagag acattacttt atgcttggtt gatagacgaa gctctttaaa ttctttgata
      421 aattcatccg catagatatc gaggttttga ttatacggtt tcaattcaat catgattttt
      481 tgatctagtg actgagcggt cttcataaat tctgccaatg atggaatgta actagcaaaa
      541 tgcccttggt gaattgggat ttttgtaact tgatctaaag ttagtttaga aacatctttg
      601 ttcataccag agagtcgttt gagattatca tcatgaatga ctactaattt atgatctttt
      661 gtcatgataa catccatctc tgaataatct gcttttgctt tatgggctgc ttttaatgct
      721 tcaatagaat tttcaacgcc atgtgatgta tcactacgat gggctattaa ttctttttta
      781 attgaaatgt tatcttgtaa gagagccaga gctatagcat tttttaaggc agtggtacca
      841 atagctagag ctagaataac acaagcaatg tgtttaaatt tttgcttatg gtggacgcct
      901 ttccaagctc tccattgttg aacaatactt ttattttgtt taatatctgc taataggtga
      961 cttatgatta aacatttaat aagaagtgtt acggcaaaaa gtattgattt taaaaggtta
     1021 tagaaagtgg cttgaagagg aaaattgttt ccagaatgat caatgaatga aaatgtacca
     1081 gcaacaacag ctagtgcaac tgttccgatt acagatataa ataaactggt taaaataatt
     1141 ttacttagaa gacgccactg acgctttccg cggctaagtt tccaactctc taaaagtgct
     1201 tctttagcat tgtagtcagt taaaatggtc agcgggagaa aataaattaa tcgaaggttt
     1261 aagtaacaaa taagcagtaa aagggtagag taacctatct ttccaaagac tgtctttgtt
     1321 aactcgccag aaataaagtc aggaatttta agatgctcta atatacttga ccttagacca
     1381 aggttagcta gaggaattgt caaaagtaag tagattgata cccaaattag ctgtggtcct
     1441 aaaagtgatt ttattttttt aggaaagatt gttagccatt ttttgatact gagagaaaat
     1501 tcagttttca gtatggcaat agtatagata gcgaactcag tatagatgaa cccaattagg
     1561 attacgatat aaagtataaa gagtaataaa gctaagggat gattaaaaag caaagtccaa
     1621 ttgtctttag tcactgcggg agtatttgat aatatcaata tccgacgaaa gataaaactc
     1681 agaattcctt ttactaaata agcaactaaa gtttggatta agataccagt tccaatgtaa
     1741 acccaaaatt gattttttaa agaggttaag gttagaaaaa aatcttcttt taatgctcgt
     1801 agcatgaata gctccttttt tatagtcatt agatattgta ccacaagttt gtttgttttt
     1861 tatggtagtt ttcaaaccgg tttcactttt ttatcaattt tgccttcaaa agctgaaacg
     1921 cattttaaaa agtgatagaa tagtagggaa aatcaaatag aataggaggt ttttatgaaa
     1981 tttctagaat taaataagaa gcgccatgct gtgaaacatt ttaatgataa gccagtcgac
     2041 tttaaagatg ttagaactgc tattgaaatt gcgacattag cgccatctgc taataatatt
     2101 cagccttgga agtttgttgt tgtccaagaa aaaaaatctg ctctagcaga aggtttaccg
     2161 gaaagtaatt gtaaccaaat taatcaagcg cagtatgtta ttgctttatt tacagataca
     2221 gatttaggac agagatcacg gaaaatagct cgtattggtc gtagatctct tcctgatgat
     2281 ttaattggtt attatatgga aactctacca ccacgctatg ctttatatag tgaaaaacaa
     2341 actggagagt atctctcttt aaatgcaggt atcgttgcta tgaatttagt acttgccttg
     2401 acagatcaag gtattagttc taatatgatt ttagggttcg ataaagcgat taccaatgat
     2461 gtcttagaga tagataaacg ttttagacca gaaatattaa ttactgttgg ttatagtgat
     2521 gaaaaggttg agcctagtta tcgtttgcca gttgatcata ttattgaaaa acgttaattt
     2581 ttaagcacaa actacataat attttataga aggagttgcc gtaatgacaa ttgattttag
     2641 agcagaagta gacaaacgca aagacgcttt gatggacgat ttaataaatc ttcttcgtat
     2701 taactcggag cgtgatgatt cacaagctga tgcagagcat ccatttggtc ctggacctgt
     2761 aaaagccttg gaatttttcc ttgagatggc tgagcgagat ggctacgaaa caaaaaacgt
     2821 agataattat gcgggacact ttacatttgg tcaaggcgaa gaagaactag gtatttttgg
     2881 gcatttagat gttgttcctg caggcagcgg ttgggataca gacccttatg aaccagttat
     2941 caaggataac cgtttatatg ctcgagggtc atcagacgat aagggaccaa caatggcatg
     3001 ttattatgct ctaaaaatta ttaaagagct tgggctacca acatctaaga aagttcgttt
     3061 tgttgtagga actgatgaag aatctggatg gggagatatg gattactact tcgaacatgt
     3121 tggtcttcca aaacctgatt ttggtttctc tccagatgca gaatttccaa ttataaatgg
     3181 tgaaaaaggt aatatcactg aatatttaca tttttcagga gaaaataagg gagctgttag
     3241 actgcatagc ttttctggcg gacttcgcga aaacatggtt ccagaatcag ccacagcacg
     3301 tttcacaagt catttagatc agacaacact tggagctagt ctagctgatt ttgcaggtaa
     3361 acataatttg aaagcagaat tatctgtaga agatgggcag tatatagcta cagtttatgg
     3421 taaatctgct catggttcaa taccacaaga aggtgttaat ggggcgactt atttagctct
     3481 ttatctaagt caatttgatt ttgaaggtcc tgctcgtgct ttcttagatg tcacagccaa
     3541 cattattcac gaagacttct caggtgaaaa acttggagta gcttatgaag atgactgtat
     3601 gggatcgttg agcatgaatg caggtgtctt ccagtttgat gaaactaatg atgataatac
     3661 tatcgctctt aatttccgtt acccacaagg gacagatgct aaaactatcc aaactaagct
     3721 tgagaaactt aacggagttg aaaaagtgac tctttctgac catgaacaca caccacacta
     3781 tgtacctatg gacgatgaat tagtatcaac cttactagct gtctatgaaa agcaaactgg
     3841 tcttaaagga catgaacagg ttattggtgg ggggacattt ggtcgcttac ttgaacgggg
     3901 tgttgcatac ggtgccatgt tcccaggaga tgaaaacact atgcatcaag ctaatgagta
     3961 catgccttta gaaaatattt tccgttcggc tgctatctac gcagaagcta tctatgaatt
     4021 aatcaaataa aataatcctt aaactaaata tgtgatcaat gataaagggt ggtgaagaca
     4081 tgaaagtgtc tttgcctctt ttcataaggt tagatttgga gactttatga ctgacttgga
     4141 aaaaattatt aaagcaataa aaagtgattc acagaatcaa aattatacag aaaatggtat
     4201 tgatcctttg tttgctgctc ctaaaacagc taggatcaat attgttggac aagcacctgg
     4261 tttaaaaact caagaagcaa tactctattg gaaagataaa tctggagatc gtctacgcca
     4321 gtggcttgga gttgatgaag agacatttta ccattctgga aaatttgctg ttttgccttt
     4381 agatttttat tacccaggca aaggaaaatc aggagattta ccccctagaa aaggttttgc
     4441 ggagaaatgg caccctctta ttttaaaaga aatgcctaat gttcaattga ccttgctagt
     4501 tggtcaatat gctcagaaat attatcttgg aagctccgca cataaaaatc taacagaaac
     4561 agttaaatct tacaaagact atctacccga ttatttaccc ctggttcacc catcaccgcg
     4621 aaatcaaatt tggctaaaga agaatccatg gtttgaaaaa gatctaatcg ttgatttaca
     4681 aaagatagta gcagatattt taaaagatta aggataggag ttagtatgag agataatcat
     4741 ctacacacgc atttttccta tgattgtcaa acggcatttg aggactatat taatggtttt
     4801 acaggtgaat ttatcacaac agaacatttt gatttatcaa atccttacac cggtcaagac
     4861 gatgttcctg attatagtgc ttattgtcaa aaaatagatt atcttaatca gaaatatgga
     4921 aatcgattta aaaaaggaat tgaaatcggt tattttaaag atagggaatc agatatttta
     4981 gattatttaa aaaataaaga atttgattta aaactattgt caatccatca taatggtagg
     5041 tatgattatc tgcaagaaga agctctgaaa gtaccaacaa aggagctttt agcagattac
     5101 tttaatcgta tggaatttgc cataggccgt gtggaagcgc acgttttaga tcactttgat
     5161 tatggttttc gtaagttaaa cttagatgta gaagatttaa aaccgtttga aacgcaattg
     5221 aagcgcattt tcagaaagat gttatctaag gggttagctt ttgaactaaa taccaaatcc
     5281 ctttatctat atgggaatga aaaactttat cgctatgctt tagagatact aaaacagctt
     5341 ggttgtaaac aatactctat aggctctgac ggtcatattc ctgaacattt ttgttatgaa
     5401 tttgatagac ttcaatgtct gctaaaggac tatcaaattg atgaaaatca tttgatatga
     5461 ggaaattttt gataaaaaag ctaggcaata ttgcttagct tttttgtaat gtcattgata
     5521 gttttagtga aaatttcaaa aaaataaaga aatcacttac ttgttgcaag cgcttgcgta
     5581 aattgttatg attttattgg taacaattca ttaaaaaagg agaatgatat gaaaagaaaa
     5641 gacttatttg gtgataaaca aactcaatac acgattagaa agttaagtgt tggagtagct
     5701 tcagttgcaa caggggtatg tatttttctt catagtccac aggtatttgc tgaagaagta
     5761 agtgtttctc ctgcaactac agcgattgca aagtcgaata ttaatcaggt tgacaaccga
     5821 caatctacta atttaaaaga tgacataaac tcaaactctg agacggttgt gacaccctca
     5881 gatatgccgg ataccaagca attagtatca gatgaaactg acactcaaaa aggagtgaca
     5941 gagccggata aggcgacaag cctgcttgaa gaaaataaag gtcctgtttc agataaaaat
     6001 accttagatt taaaagtggc accatctaca ttgcaaaata ctcccgacaa aacttctcaa
     6061 gctataggtg ctccaagtcc gaccttgaaa gttgctaatc aagctccaca gattgaaaat
     6121 ggttacttta ggttacatct taaagaattg cctcaaggtc atcctgtaga aagcactggg
     6181 ctttggatat ggggagatgt tgatcaaccg tctagtaatt ggccaaatgg tgctatccct
     6241 atgactaatg ctaagaaaga tgattacggt tattatgttg attttaaatt atctgaaaaa
     6301 caacgaaaac aaatatcttt tttaattaat aacaaagcag gaacaaattt aagcggcgat
     6361 catcatattc cattattacg acctgagatg aaccaagttt ggattgatga aaagtacggt
     6421 atacatactt atcagcccct caaagaaggg tatgtccgta ttaactattt gagttcatct
     6481 ggtaactatg accacttatc agcatggctc tttaaagatg ttgcaacccc ctcaacaact
     6541 tggccagatg gtagtaattt tgtgaatcaa ggactatatg gaaggtatat tgatgtacca
     6601 ctgaaaacta atgccaaaga gattggtttt ctaatcttag atgaaagtaa gacaggagat
     6661 gcagtgaaag ttcaacccaa cgactatgtt tttagagatt tagctaacca taaccaaatt
     6721 tttgtaaaag ataaggatcc aaaggtttat aataatcctt attacattga tcaagtgcag
     6781 ctaaaggatg ctcaacaaac tgatttaaca agtattcaag caagttttac aactctagat
     6841 ggggtagata aaactgaaat tttaaaagaa ttgaaagtga cagataaaaa tcaaaatgct
     6901 atacaaattt ctgatatcac tctcgatact agtaaatctc ttttaataat caaaggcgac
     6961 tttaatccta aacaaggtca tttcaatata tcttataatg gtaacaatgt cacgacaagg
     7021 caatcttggg aatttaaaga ccaactttat gcttatagtg gaaatttagg tgcagttctc
     7081 aatcaagatg gttcaaaagt tgaagccagc ctctggtcac cgagtgctga tagtgtcact
     7141 atgattattt atgacaaaga taatcaaaac agggttgtag cgactacccc ccttgtgaaa
     7201 aataataaag gtgtttggca gacgatactt gatactaaat taggtattaa aaactatact
     7261 ggttactatt atctttacga aataaaaaga ggtaaggata aggttaagat tttagatcct
     7321 tatgcaaagt cattagcaga gtgggatagt aatactgtta atgacgatat aaaaacggct
     7381 aaagcagctt ttgtaaatcc aagtcaactt ggacctcaaa atttaagttt tgctaaaatt
     7441 gctaatttta aaggaagaca agatgctgtt atatacgaag cacatgtaag agacttcact
     7501 tctgatcaat ctttggacgg aaaattaaaa aatcaacttg gtacctttgc agccttttca
     7561 gagaaactag attatttaca gaaattagga gttacacaca ttcagctttt accggtattg
     7621 agttattttt atgttaatga aatggataag tcacgctcaa cagcttacac ttcctcagac
     7681 aataattaca attggggcta tgacccacag agctattttg ctctttctgg aatgtattca
     7741 gagaaaccaa aagatccatc agcacgtatc gccgaattaa aacaattaat acatgatatt
     7801 cataaacgtg gcatgggggt tatacttgat gtcgtctata atcacactgc aaaaacttat
     7861 ctctttgagg atatagaacc taattattat cactttatga atgaagatgg ttcaccaaga
     7921 gaaagttttg gagggggacg tttaggaacc actcatgcaa tgagtcgtcg tgttttggtt
     7981 gattccatta aatatcttac aagtgaattt aaagttgatg gtttccgttt tgatatgatg
     8041 ggagatcatg atgcggctgc gattgaatta gcttataaag aagctaaagc tattaatcct
     8101 aatatgatta tgattggtga gggctggaga acattccaag gcgatcaagg taagccggtt
     8161 aaaccagctg accaagattg gatgaagtca accgatacag ttggcgtctt ttcagatgat
     8221 attcgtaata gcttgaaatc tggttttcca aatgaaggta ctccagcttt catcacaggt
     8281 ggcccacaat ctttacaagg tatttttaaa aatatcaaag cacaacctgg gaattttgaa
     8341 gcagattcgc caggagatgt ggtgcagtat attgctgcac atgataacct taccttgcat
     8401 gatgtgattg caaaatcaat taataaagac cctaaggtag ctgaagaaga tattcataga
     8461 cgtctgcgtt taggaaatgt aatgatttta acatctcaag ggacagcatt cattcattct
     8521 ggtcaagagt atggtcgtac gaagcgttta cttaaccctg attacatgac aaaagtttca
     8581 gatgacaaat tgcctaataa agcaacactt attgaagctg ttaaagaata cccatatttt
     8641 attcatgatt catatgattc ttcagatgcc attaatcatt ttgattgggc agcagccaca
     8701 gataataaca aacacccaat ttcaacgaaa acacaggcct atacagcagg tttaatcaca
     8761 ttaaggcgtt caacagatgc tttccggaaa ttgagcaaag cagaaattga tcgtgaggtt
     8821 agcttgatta cagaggtagg tcaaggtgat attaaagaaa aagatttggt tattgcttac
     8881 caaacaatag attctaaagg cgatatttac gcagtatttg ttaatgctga tagtaaagct
     8941 agaaacgttt tactaggtga aaaatataaa caccttttaa aagggcaagt aattgttgat
     9001 gctgatcaag cggggattaa accaatctca actcctagag gtgttcattt tgaaaaagat
     9061 agtttgctga ttgatccatt aacagcaatt gtgattaaag ttggcaaagt tgctcctagc
     9121 cctaaggagg aattgcaagc agattatccc aaaacacaat ctttcaaggg atctaaaacg
     9181 gtagaaaaag taaatagaat agctaataag acctcaataa ctcctgtagt ttctaataag
     9241 accgattcat atctgacaaa tgaagctaat ttgccaaaaa ctggagataa gtcatcaaaa
     9301 atactaagtg tagtaggaat aagcattcta gcaagtctac ttgctctact aggtctctct
     9361 ttaaagagga atcgtcactt aaaataaaag caaactttag aagagcttaa aaaagaagtt
     9421 agtcaatggg ctagcttctt ttcattaagt taataaaatc acttatcagc aaaatctaat
     9481 tgtgttataa tggatgtaaa cgttttttta tgaggtatat gatgaaaaat tgggaaaaac
     9541 caccattagt gatggcggga ttagtattgg ggctattagc tttggggaac cttctagagg
     9601 gatatggcac ctattttcgc tattgtttag ggctagtagc acttgttttt tggatttttt
     9661 taatcaaagg aatattaaaa aataaaaaag aaagcaggaa ggagttaagc aatcctttga
     9721 ttgcttctgt ctttactact ttttttatgg caggtatgat tttatcgacc tatatcttat
     9781 tatttagaag tttaggaata tgggtagcag ttctgtcaaa aggtgtatgg tggttgtcat
     9841 ttattgcgtt aatcattcat atggctattt tttcatggaa atatttgaga cacttttcga
     9901 tggctaatct ttttccttct tggtctgtcc tctatgttgg tataggggta gctagcctga
     9961 cagcaccaat tagtggacaa ttcactattg ggaaaatagt tttttggtat ggttttattg
    10021 caactttagt attattacca tttctattca ttaaagcata taaaatcggg ctaccttcgg
    10081 cagttaagcc taatataact actatttgcg ctccaatgtc gctaatcaca gcgggatatg
    10141 ttaatagttt tgtgtcacca aatagaggtt tattattgct attaatcgta atggcacaat
    10201 ttctttattt tttcattctt tttcaggtgc ctaaattact gattggtgat tttacaccag
    10261 gtttctcagc ctttactttc ccccttgtta tctcagctac ctcacttaaa ttaagtattc
    10321 agcatctatc attaccagtt gatatacagg ggctagtcca ctttgaaatt gggacaacaa
    10381 ctcttattgt tatgatagta atggtacgtt atattttctt tttaaggcga acaatttaat
    10441 ataaaataac tcccttacta gctagtaagg gagttattgt ttaatgagag acacgtttac
    10501 gtgcagcttt tttacgattt tcttcgataa aagctatttt ttcttcctca ggttcaataa
    10561 ttgttttttt gatagcaaat gcagcacctg caagagtagc agctgttgca acgactcctg
    10621 ttgctattcc tttagcaaat gcgaattttt taaccataat aattctccaa tctatgatga
    10681 agactctgct tctatctatg atataattat ggtaacgaaa tactagttaa atttcaagta
    10741 aaaaccttta tagaattgag aagtatcatg agaaaaatca agcttatagc agttgttggt
    10801 ccaactgcgg ttgggaagac agcattagga attgaattag ctaaaacttt caatggcgaa
    10861 atcattagtg gcgatagtca gcaagtttat cagaagttag atatcggaac ggctaaagct
    10921 agtaaagaag agcaagaaca agcttatcat cacttgattg atgttcgtga agtaaatgag
    10981 aattattcgg tttatgattt tgtcaaagag gcaaaagtag ctattgatac gattatttct
    11041 aaggggaaaa tccctattat tgttggaggt actggcctat atttacagag tttgtttgag
    11101 ggctatcatt taggaggcga agtcaatcaa gaaactctta aggcctatcg tgaaaaatta
    11161 gagtctctaa gtgatgaaga tttatttgaa aagttgacag aacaatccat tgttataccg
    11221 caagttaatc gtcgacgtgc catccgtgca ttagaattgg cgaagtttgg gaatgatttg
    11281 caaaatagtg aaagtccgta tgatgtttta ttaattggat tgaatgatga tcgtcaggtg
    11341 ctctatgatc gtatcaatag acgagttgat ttgatgatag acaatggtct cttagatgaa
    11401 gctaaatggc tatacgataa ctaccctagt gttcaagcaa gtagaggaat tggctataaa
    11461 gaattatttc cttacttttc aaagcaaata ccattagaag aagctgttga taaactaaaa
    11521 caaaatacca gacgttttgc aaaacgccaa ttaacatggt ttagaaaccg tatgaatgtc
    11581 gaatttatta tggttggtga ggaaaattat caacaaaaaa tcaagcgaaa agtcagtgat
    11641 tttttatcat caaagtaaat aaagccgttt ctcgcattta aagggcatta tgttataata
    11701 aagattacgt atttagaaca agaaaaaaat attgaaagga gattataggt gatagaaact
    11761 aaagaagagc aagaacgtgt tattttagtt ggggtagaac ttcaagatac tgagaatttt
    11821 gaaatgtcca tggaagaact tgctagctta gctaagacag caggagcaaa tgttgttaat
    11881 cattattatc aaaaaagaga taaatatgat agtaaatcat ttatcggatc tggtaaatta
    11941 gaggagataa aagctattgt agaagcagat gagattgata ccgttgtggt taataatcgt
    12001 ttgactccgc gacaaaactc taatttggaa gctgaacttg gtgttaaagt tattgaccgt
    12061 atgcaactta ttttagatat ctttgctatg cgtgctagaa gtcatgaagg aaaattacag
    12121 gttcatctag cgcaattgaa atatatgtta ccacgcttag ttggacaagg tattatgctt
    12181 agtcgtcaag caggaggaat cggaagccga ggtccaggag aatctcagtt agagttgaat
    12241 cgtcgttcca ttcgtcatca aatttctgat atcgaaagac aattgaagat tgtagaaaaa
    12301 aatcgcgaga cagttcgaga aagacgtgtt gattcgacaa cctttaaaat tggattaatt
    12361 ggatatacaa atgctggcaa gtcaactatt atgaatgttt tgaccgatga taagcaatat
    12421 gaagccaatg aattatttgc aacgttagac gctacaacca agcagatcta tttacaaaat
    12481 caatttcaag tcactttaac agatactgtt ggttttattc aagatttacc tacagagtta
    12541 gttgcggctt tcaagtctac cttagaagaa agtcgacatg ttgatttatt acttcatgtt
    12601 attgatgcta gtgaccctaa tcatgaagaa cacgaaaaag ttgttatgga gattcttaaa
    12661 gacttagata tgatagatat tcctcgttta gcgatataca acaagatgga tgtcactgag
    12721 cagctgaatg caacaacttt tcctaatgtg agaattgcag ccaaaaaaca agggagtagg
    12781 gatttattac gccgtcttat tgtggatgaa atacgacata tctttgacga attttcgatt
    12841 agagtgcatc aaaatcaagc atataagctc tatgatctca ataaaatagc attgcttgac
    12901 acctacacct ttgaagaaga atacgagaat attacaggct atatttctcc taaacaaaaa
    12961 tggaagctgg aagaatttta tgattgatta tattgatttg gctttgactt atggtggttt
    13021 tacgagctta gataaagttt atctagagaa aaaactagat ggcctctcta agcaacaacg
    13081 acttgatttt atcactccac ctcctagtgt gattaatgct tattttgctg aaatatatca
    13141 aaagcaaggg ccagaagcgg caactgatta ctattttgat ctttccaaag ccttgggctt
    13201 gtttccaaaa catttatcgt ttgatgaaga aaaacctttt attcgtttga atttatcagg
    13261 aaaatcattt ggttttgctt acctaaatga tcaagaagaa gcgagtgttt tttctgaagt
    13321 taaagaagtc attacaccgc agttactctt agaaattgca cagatttttc ctcaatataa
    13381 agtctatcaa gatcggtctg gtatcagaat ggcaaaaata gattttgacg aaacagaatc
    13441 tcaaaatatt acaccagaaa caagtttact agggaatgtc ttacaactta aaaaagatat
    13501 tattaaaata actagtttca atcaagaaga gctattagag
//
LOCUS       DBJYSH010000056        13027 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.056, whole genome shotgun sequence.
ACCESSION   DBJYSH010000056 DBJYSH010000000
VERSION     DBJYSH010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 13027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13027
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.056"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            291..1907
                     /gene="nikA"
                     /locus_tag="ACUBV3_001223"
     CDS             291..1907
                     /gene="nikA"
                     /locus_tag="ACUBV3_001223"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001253836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter substrate-binding
                     protein"
                     /protein_id="HHU3847727.1"
                     /translation="MRRNILLSITCLLMVTLTACHSQDSKSHKLNSDKLTLAWGEDFG
                     DVNPHRYNPDQFVIQDMVYEGLVRYGDNGKIEPALAKSWSISQDGKTYTFKLRNAKYS
                     DGSNFNAANVKRNFDSIFSKSNRGNHNWFNLTNQLENYRALNQSTFEIKLKQAYSATL
                     YDLSMIRPIRFLSDSAFPKGDDTTKKNVKKPIGTGQWVVKSKKQNEYITFKRNENYWG
                     KKPKLKEVTVKVIPDAQTRALAFESGDVDLIYGNGIIGLDTFAQYTKDKKYVTAISQP
                     MSTRLLLLNAKESIFQDKKVRQAMNHAIDKVSIAKNTFRGTEKPADTIFSKSTSHSDA
                     KLNPYSYNVDKANQLLDQAGWKMGKDKVREKDGKALTLRLPYIATKATDKDLVTYFQG
                     EWRKIGINVSLIAMEEDDYWANAKKGNFDMMLTYSWGAPWDPHAWMSALTAKADHGHP
                     ENIALENLATKTEMDRLIKSALVDPKEENVDRDYKKVLELLHDEAVYIPLTYQSVISV
                     YRKGDFKTMRFAPEENSFPLRYIEKNNVSK"
     gene            1894..2838
                     /locus_tag="ACUBV3_001224"
     CDS             1894..2838
                     /locus_tag="ACUBV3_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HHU3847728.1"
                     /translation="MYLSSSIIKKILSAFLALFFISLLTFILIKLSTVNPAENYLRLS
                     KISVSPEALKEAEHYLGLDKPLWKQYWLWFQKALTGDFGYSYVLRLPVLDLVLQRFLA
                     TLFLGTSAFLLIVTISTPLGVWAGLHESARSDHLIRFLSFSSVSMPNFWVAYLLMLLF
                     SAKLNLLPVSGGNDLQSLILPSITLSFSTVGQYIALIRKAISQENRSLNVENARLRGV
                     KERYIVTHHLLRNALPAIMTALSLTWVYLLTGSIIVEEIFSWNGIGRLFVTSLRTSDL
                     PVIQACMLIFGTLFLANNFMTQCFMNWVDPRLRKSREK"
     gene            2838..3644
                     /locus_tag="ACUBV3_001225"
     CDS             2838..3644
                     /locus_tag="ACUBV3_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HHU3847729.1"
                     /translation="MSKRIYFSSSLLVLLVISAIFAPILSSFDPQYVDLSQKLLAPNN
                     VHLLGTDQLGRDVLSRLLYGARYSLFLAIIISLLELTIGMFVGLIVGWYQGKLENLFL
                     WIANIILAFPSFLLSLATVGILGHGLGNLIFAIVFVEWVYYAKLMTNLVKSAKKEPYV
                     INAQIMGLSVWHILRKHIFPFVYQPILVMVLMNIGNIILMISGFSFLGIGVQPNVTEW
                     GMMLHDARGYFRTATWMMLSPGIAIFLTVFSFNTLGDAIDKKDWKRQWNS"
     gene            3632..4420
                     /locus_tag="ACUBV3_001226"
     CDS             3632..4420
                     /locus_tag="ACUBV3_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HHU3847730.1"
                     /translation="MEQLEIRKLSLQIGEVPVLRDFSCKIDMGESLTIIGESGSGKTL
                     LAKLLVGHIPQGMTVRGNIFFKGVDLGKLTVKQWQKLRGRDIAYLVQNPMSMFNPFQK
                     IEAHILETILSHEKCSKRVALSKALEWMKRLNLDDAISLLKKYPFELSGGMLQRIMLA
                     TILSLDPQVIILDEPTSAVDCHNCSTISAILQELQNNGKTLITVTHDYQLARDLGGQL
                     LVISEGEVVEQGQTQAILSNPQHNYTKALTVQMEYEGDILNVGL"
     gene            4407..5087
                     /locus_tag="ACUBV3_001227"
     CDS             4407..5087
                     /locus_tag="ACUBV3_001227"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HHU3847731.1"
                     /translation="MLVCNHVGKTFGRQEVLKDCHFHLKRGEIIGIMGKSGSGKSSLA
                     RLIIGLDSPTCGSIYFQGKIYTPKDGKAQIILVFQDALSSVNPYFSIEEILNEAFYGK
                     KTTFELCQILEAVGLDGTYLKYKARQLSGGQLQRVCIARALLLKPKIIIFDESLSGLD
                     PVTQIKMLRLLQKIKRRYELSFIMISHDPKICQAICNRVFLIKNGYLVEDNEFLKRAC
                     STNCLTNL"
     gene            5208..5936
                     /gene="pyrH"
                     /locus_tag="ACUBV3_001228"
     CDS             5208..5936
                     /gene="pyrH"
                     /locus_tag="ACUBV3_001228"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HHU3847732.1"
                     /translation="MEPKYQRILIKLSGEALAGDKGVGIDIPTVQSIAKEIAEVHNSG
                     VQIALVIGGGNLWRGEPAAEAGMDRVQADYTGMLGTVMNALVMADSLQQYGVDTRVQT
                     AIPMQTVAEPYVRGRALRHLEKNRIVVFGAGIGSPYFSTDTTAALRAAEIEAEAILMA
                     KNGVDGVYNADPKKDANAVKFDELTHVEVIKRGLKIMDATASTISMDNDIDLVVFNMN
                     ETGNIKRVVLGEQIGTTVSNKASE"
     gene            5952..6509
                     /gene="frr"
                     /locus_tag="ACUBV3_001229"
     CDS             5952..6509
                     /gene="frr"
                     /locus_tag="ACUBV3_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HHU3847733.1"
                     /translation="MTKEIVTKAQERFEQSHQSLSREFAGIRAGRANASLLDRIQVEY
                     YGAPTPLNQLASITVPEARVLLISPFDKSSIKDIERAINESDLGINPANDGSVIRLVI
                     PALTEETRRDLAKEVKKVGENAKIAIRNIRRDAMDEAKKQEKNKEITEDDLKSLEKDI
                     QKATDDAVKHIDEMTANKEKELLEV"
     gene            6627..7481
                     /gene="cvfB"
                     /locus_tag="ACUBV3_001230"
     CDS             6627..7481
                     /gene="cvfB"
                     /locus_tag="ACUBV3_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding virulence regulatory protein CvfB"
                     /protein_id="HHU3847734.1"
                     /translation="MNTLLATVITGLVTDENKDFYFIQKDGFTFALSKSEGEHHIGEM
                     VKGFAYTDMQQKARLTTKETFATRDHYGWGTVTEVRKDLGVFLDTGLPDKQVVVSLDV
                     LPELKELWPKKGDRLYVCLDVDKKDRLWALPADPEVFQRMATPAYNNMQNQNWPAIVY
                     RLKLSGTFVYLPENNMLGFIHPSERYSEPRLGQVLDARVIGFREVDRTLNLSLKPRSF
                     EMLENDAQMILTYLESNGGFMTLNDKSSPEEIKATFGISKGQFKKALGGLMKAKKIKQ
                     DQLGTELL"
     gene            7619..8128
                     /gene="msrA"
                     /locus_tag="ACUBV3_001231"
     CDS             7619..8128
                     /gene="msrA"
                     /locus_tag="ACUBV3_001231"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HHU3847735.1"
                     /translation="MERAIFAGGCFWCMVQPFEELDGIESVLSGYTGGHVENPTYKEV
                     CSKTTGHTEAVEIIFNPEKISYADLVELYWAQTDPTDAFGQFEDRGDNYRPVIFYENE
                     EQRQIAQKSKDKLQASGRFDRPIVTSIEPADTFYPAEDYHQAFYRTNPARYALSSARR
                     HAFLEENWH"
     gene            8125..8340
                     /locus_tag="ACUBV3_001232"
     CDS             8125..8340
                     /locus_tag="ACUBV3_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HHU3847736.1"
                     /translation="MRKSFYSWLMTQRNPKSNEPVAILADYAFDETTFPKHSSDFETV
                     SRYLEDEASFSFNLTDFDDIWEDYLNH"
     gene            8497..10269
                     /locus_tag="ACUBV3_001233"
     CDS             8497..10269
                     /locus_tag="ACUBV3_001233"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HHU3847737.1"
                     /translation="MRYTNGNFEAFARPRKPEGVDKKSAYIVGSGLAGLAAAVFLIRD
                     GQMDGQRIHIFEELPLSGGSLDGVKRPDIGFVTRGGREMENHFECMWDMYRSIPSLEV
                     PDASYLDEFYWLDKDDPNSSNCRLIHKQGNRLESDGDFTLGTHSKELVKLVMETEESL
                     GAKTIEEVFSKEFFESNFWTYWATMFAFEKWHSAIEMRRYAMRFIHHIGGLPDFTSLK
                     FNKYNQYDSMVKPIISYLESHNVDVQFDSKVTNISVDFKNGQKLAKAIHLTVGGEAKT
                     IDLTPNDFVFVTNGSITESTNYGSHDTVAKPNTDLGGSWNLWENLAAQSDEFGHPKVF
                     YKDIPKESWFVSATVTIKDPAIEPYIERLTHRDLHDGKVNTGGIVTVTDSNWMMSFAI
                     HRQPHFKEQKENETIVWIYGLYSNVEGNYIKKPIEECTGREITEEWLYHLGVPEMKIH
                     DLSDKQYVSTVPVYMPYITSYFMPRVKGDRPDVIPQGSVNLAFIGNFAESPSRDTVFT
                     TEYSIRTAMEAVYTFLNIERGVPEVFNSAFDIRVLLQSLYYLNDKKSVEDMDLPIPAL
                     MRKVGMKKIRGTYLEELLREAHLL"
     gene            10393..11379
                     /locus_tag="ACUBV3_001234"
     CDS             10393..11379
                     /locus_tag="ACUBV3_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001158936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HHU3847738.1"
                     /translation="MQEYSIEITLQHPDDMMSLFGSNERHLKLIEENLDVIIHARTER
                     VQVLGDSEEAVETARLTIEALLVLVNRGMTVNTSDVVTALSMAQNGSIDKFVALYEEE
                     IIKDSYGKPIRVKTLGQKIYVDSVKNHDVVFGIGPAGTGKTFLAVTLAVTALKRGQVK
                     RIILTRPAVEAGESLGFLPGDLKEKVDPYLRPVYDALYQILGKEQTSRLMEREIIEIA
                     PLAYMRGRTLDDAFVILDEAQNTTIMQMKMFLTRLGFNSKMIVNGDVSQIDLPKNVKS
                     GLIDAVEKLRNIKKIDFIHLSAKDVVRHPVVAEIINAYSDSESSHKLQSDKD"
     gene            complement(11470..12273)
                     /locus_tag="ACUBV3_001235"
     CDS             complement(11470..12273)
                     /locus_tag="ACUBV3_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000349560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4037 domain-containing protein"
                     /protein_id="HHU3847739.1"
                     /translation="MDHFTKLWQDFSKLPNVVAIALGGSRSGDSFDQSSDYDLYVYCA
                     ATPDITSRKRILNKHCHYIELNNHYWELEDNGTLNDGTDIDILYRNIDNFLSDLEDVV
                     EHHNSRIGYTTCFWHNLINCQILYDPENQLQSLKERFEVSYPSQLQKQIIIQNRNLLT
                     GKLPSYDKQIIKALKRQDFVSTHHRTTAFLDSYFDIIFALNKLTHPGEKRMISYAKKN
                     ATLLPKHFEENIIKLCHHNSNEHTVKETLNDIIMHLDVMLKENFQHFIG"
     gene            12465..12941
                     /locus_tag="ACUBV3_001236"
     CDS             12465..12941
                     /locus_tag="ACUBV3_001236"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HHU3847740.1"
                     /translation="MKQDYISYIRSKVGHETIFLTYSGGILTDGKGRVLLQLRADKNS
                     WGIIGGCMELGESSVDTLKREFFEETGLRVEPIRLLNVYTNFQDSYPNGDKAQTVGFI
                     YEVSCPKPVNIEGFHNEETLQLDYFSKEDVKNITIVNEQHQLILDEYFSQTFQMGR"
BASE COUNT         4272 a         1937 c         2614 g         4204 t
ORIGIN      
        1 cttttgaaaa atagcttttt atctgtatta accttcttaa agactaatga atgtgcgatg
       61 tgttttaagt aacagatgag aataaggaaa aatgaaattg gcgatatggt atgtgactat
      121 tatgattatt ctttagcaat aatctattaa aatgagttcg cagggaataa gtaaattttg
      181 tatcgttgta gattttttat tttaagattc ttgacaagac attactaaaa tgatttaatt
      241 aagtttgctt aaaaaaataa ttaactaaac ttaaaagaaa gttggtgaaa atgagacgta
      301 acatattatt gagcattact tgtttattga tggtgactct gactgcatgc cattcacagg
      361 atagcaaaag tcataaacta aacagtgata aactcacgct tgcttgggga gaagactttg
      421 gtgatgttaa tccgcatcgc tataaccctg atcagtttgt tatccaggat atggtttatg
      481 aggggttggt acgatatggt gacaatggga aaattgaacc agctttagcg aaaagctgga
      541 gcattagtca agatggaaaa acctacactt ttaaattgag aaatgctaaa tattctgatg
      601 gcagtaactt taatgctgcg aatgtcaaac gtaattttga tagtattttt tcgaagtcta
      661 atagaggtaa tcataattgg tttaatttaa cgaatcaatt ggaaaattat cgtgccttga
      721 atcaaagtac ttttgaaatt aaactgaaac aagcttatag tgctacttta tatgatctct
      781 ctatgattcg accaattcgc tttttatctg atagcgcttt ccctaaaggt gatgatacaa
      841 ctaagaagaa tgtaaaaaaa ccaattggaa cgggtcaatg ggttgtgaaa agtaaaaagc
      901 aaaatgaata tattaccttc aaacgtaatg agaattactg gggaaagaaa ccaaaattaa
      961 aagaagtaac cgtaaaagtt atacccgatg ctcagacgcg tgctttagca ttcgaatcag
     1021 gtgacgttga tttgatctat gggaatggca ttattggttt agacactttt gcccaatata
     1081 cgaaggacaa gaaatatgtc acagctattt ctcaacctat gtcaacaaga cttttgttgc
     1141 taaatgctaa ggaaagtatt ttccaagata aaaaagttcg tcaggcaatg aatcatgcta
     1201 ttgacaaggt ttctattgct aagaacacct ttagaggaac agaaaagcca gcagacacta
     1261 ttttttcaaa atcaacatct cactcagatg ctaagttaaa tccttatagc tataatgttg
     1321 ataaagcaaa tcagctattg gatcaagctg gttggaagat gggaaaagat aaggttcgtg
     1381 aaaaagatgg taaagctcta accttacgtc ttccttatat cgccactaag gcaactgata
     1441 aagacctggt aacttatttc caaggagaat ggcgtaaaat cggtattaat gtttctctta
     1501 ttgctatgga agaagatgat tattgggcta atgctaaaaa gggtaatttt gatatgatgt
     1561 taacctattc ttggggagca ccatgggacc ctcatgcttg gatgtcggca ttaactgcca
     1621 aggcagatca tggacatcca gaaaatatag ctttagaaaa tctagcaact aaaactgaaa
     1681 tggatagact tattaagtca gctctagtag atcctaagga agaaaatgtt gatagagact
     1741 ataagaaggt tcttgaatta ttgcatgatg aggctgtata tatcccatta acttatcaat
     1801 cggtgatttc tgtttatagg aaaggtgatt ttaaaaccat gcgttttgcg ccagaagaaa
     1861 attcattccc attacgctat atagaaaaaa acaatgtatc taagtagttc gatcatcaaa
     1921 aaaatattgt cagctttttt ggcattgttt ttcatatcac ttctaacatt tattcttatc
     1981 aaactatcaa cagttaatcc agcagaaaat taccttcgtt tatcaaaaat aagtgttagt
     2041 ccagaagcgc taaaagaagc agagcactat ttaggtttag ataagccatt atggaagcag
     2101 tactggctgt ggttccaaaa agcattaaca ggagattttg gatattctta tgtgctcagg
     2161 ttgccagtat tagatttggt tttacaacgg tttttggcta ctttattttt aggcacgagt
     2221 gccttcttgc taattgttac catttctacc ccgttaggtg tgtgggctgg cttacatgaa
     2281 tctgctcgga gtgatcactt gattcgattt ttgagttttt ctagtgtgtc tatgccaaat
     2341 ttttgggttg cttacttgtt gatgctgcta ttttcggcaa aattaaatct tctgcctgtt
     2401 tcaggaggta acgatttaca aagtttaatt ttgccaagta ttaccttaag tttttctacc
     2461 gtaggtcaat acatagccct tattcgaaaa gctattagtc aagaaaatag aagtttaaat
     2521 gtggaaaatg cccgattaag aggagtaaaa gaacgttata ttgtcacaca tcacctcctt
     2581 agaaatgcct tacctgcgat aatgacagca cttagcttga cttgggttta cttattaaca
     2641 ggatcaatta ttgttgaaga aattttttca tggaatggaa taggacgttt atttgtgacc
     2701 agtctaagaa cttcagatct tccagtaata caagcttgta tgctaatttt tggaacctta
     2761 tttttagcta ataattttat gacacagtgt tttatgaatt gggttgatcc tcggttacga
     2821 aagtcaaggg agaaataata tcaaaaagaa tttatttttc gagtagctta ctggtattgt
     2881 tagtgattag tgccattttt gcccctattc tatctagctt tgatccacaa tacgtagatt
     2941 tatcacaaaa attattggct cccaataatg ttcatttgtt agggactgac caattgggta
     3001 gagatgtatt atctcgcttg ctttatggtg ctagatattc actgttttta gctattatta
     3061 ttagcttatt ggaattaacg attggtatgt ttgtgggtct tattgttggt tggtatcaag
     3121 gaaagttaga aaacctcttt ttatggatag ctaatattat tttagctttt ccgagctttt
     3181 tgctctctct tgcaaccgtg gggatcttag gtcatggtct agggaattta atttttgcaa
     3241 ttgtctttgt ggaatgggtt tactatgcta aattaatgac caatttagtt aagagtgcaa
     3301 aaaaagagcc ttatgtgata aatgcgcaaa ttatgggact ttcagtctgg catattttaa
     3361 gaaaacatat ttttcctttt gtgtatcagc caatcctcgt tatggtcctt atgaatatag
     3421 gaaatattat tttaatgatt tctggctttt ctttcttagg gattggtgtg caacctaatg
     3481 tcacagaatg gggaatgatg ttgcacgatg ctagaggata ttttaggaca gctacttgga
     3541 tgatgttatc tcctggaatt gcgatttttt taacagtatt ttcctttaat actttaggcg
     3601 atgccataga taaaaaagat tggaaacgac aatggaacag ttagaaataa gaaaattgtc
     3661 attacagata ggagaggtcc ctgtactaag agattttagt tgtaaaatag atatgggaga
     3721 atcgctaact attattggtg agagtggttc agggaaaacc ctcttggcaa aattattggt
     3781 tggtcacatt ccacaaggta tgacagttag aggaaacata ttttttaagg gagttgattt
     3841 aggtaaacta actgtaaagc agtggcagaa attaagggga cgagatattg cctatttagt
     3901 gcaaaatccc atgtctatgt ttaatccttt tcaaaaaatt gaagcgcata ttttggaaac
     3961 aatccttagt catgagaaat gttcaaagag agtagcctta tctaaagcgt tagaatggat
     4021 gaaacgttta aatttagatg atgcgatatc cctgttaaaa aaataccctt ttgagcttag
     4081 tggaggaatg ctacaaagaa ttatgttagc cactatatta tctcttgatc ctcaggtaat
     4141 catattagat gagccgacct cagcggttga ttgtcataat tgttctacta tatcagctat
     4201 tttgcaggaa ttgcaaaata atggaaaaac cttgattact gtaacgcatg attatcagtt
     4261 agctagagac ctggggggac aattattagt tataagtgag ggagaagttg ttgaacaggg
     4321 acaaacccaa gctattttaa gcaatcctca acataactat acgaaagcac tgacagtgca
     4381 aatggagtat gaaggagata ttcttaatgt tggtttgtaa tcatgtcgga aaaacatttg
     4441 gacgacaaga ggttttaaag gactgtcatt ttcatcttaa aagaggagaa ataattggta
     4501 taatgggtaa aagtggcagt ggtaaaagta gccttgcccg actcattata ggactagata
     4561 gtcctacttg tggttcgata tattttcaag gaaaaattta cacacctaaa gatggtaagg
     4621 cgcagattat ccttgttttt caagatgcgc ttagttcggt caatccatat tttagtattg
     4681 aggaaatttt gaatgaagct ttttatggaa aaaaaacaac ttttgaatta tgtcaaatat
     4741 tagaagcggt gggtttagat ggaacttatc taaaatataa agctagacaa cttagtggtg
     4801 gtcagctaca gagagtttgt atcgctagag ctttactctt aaagcctaaa attattattt
     4861 ttgatgaatc cttaagtgga ttagacccag taactcaaat taaaatgcta cgtttattac
     4921 aaaaaataaa acgccgttat gaattaagtt ttataatgat ttctcatgac cctaaaattt
     4981 gtcaagctat ctgtaaccgg gtctttctga taaaaaatgg ttatttagta gaagataatg
     5041 aattcttgaa gagagcgtgt tctactaact gtttgaccaa tctctgaatt attatgtttt
     5101 tcttatttta ggagttgata aagaaatcaa cttactagtg tcattgctct attaaaattt
     5161 tgccttttat ggtaaaataa taaagacaaa ataaggagaa catagtagtg gaacctaaat
     5221 atcaacgtat attaattaag ttatctggtg aagcattggc gggagataag ggtgtcggta
     5281 ttgacattcc taccgttcaa tctattgcaa aagaaattgc cgaagtacac aattcaggtg
     5341 ttcaaatagc acttgtcatt ggtggaggta atctttggcg tggagaacct gcagcagaag
     5401 cagggatgga tcgtgttcaa gctgattata cagggatgtt agggacagta atgaatgctc
     5461 ttgtaatggc tgatagcctt caacaatatg gggtagatac tagagttcaa acagctattc
     5521 caatgcaaac tgttgctgag ccttacgtac gtggtcgtgc tttacgtcat ctagagaaga
     5581 atcgtattgt tgtctttgga gcgggtattg gttcgcctta tttctcaact gatacaacag
     5641 cagcgcttcg tgctgctgag attgaggcag aagcaatttt aatggctaaa aatggagtag
     5701 atggtgttta taatgctgat ccaaagaaag atgccaatgc tgtcaaattt gatgaattaa
     5761 ctcacgttga agtaataaaa cgagggttaa aaatcatgga tgcaaccgca tcaactattt
     5821 caatggataa tgatattgac cttgttgttt tcaatatgaa tgagactggt aatattaagc
     5881 gtgttgttct tggagaacaa atcggaacaa ctgtttcaaa caaagcatct gaataggagt
     5941 gtataaaaaa tatgactaag gaaatcgtta caaaagctca agagcgtttt gagcaatcac
     6001 accaaagttt atcaagagaa tttgcaggga ttcgtgcagg acgtgctaat gctagcttgc
     6061 ttgatcgtat tcaagttgag tattatggag cgccaacccc acttaaccaa ttagcttcta
     6121 ttactgtacc tgaagctcgt gttcttttaa tttcaccatt tgataaatca tcaatcaaag
     6181 atattgagcg tgctattaac gaatcagatt taggtattaa tcctgcaaat gatggttcag
     6241 ttattcgtct tgttattcca gctttgacag aagaaacacg tcgtgattta gctaaagaag
     6301 tgaaaaaagt cggagaaaat gctaagattg ctatccgtaa tattcgccgt gatgctatgg
     6361 atgaagctaa gaagcaagaa aagaataaag aaattacaga agatgacttg aaatctcttg
     6421 aaaaagatat tcaaaaggct actgatgatg ctgtaaaaca tattgatgag atgactgcta
     6481 ataaagaaaa agaattgttg gaagtttaat agtaacgttt gctatcttta ttatgattgg
     6541 caagtaataa aatgattatg gaggtaactg atagaatcaa aaccagatgg atttggtttg
     6601 tcggttacct ttttaaaagg agaaaaatga atacattatt agcgacagtc ataacaggct
     6661 tagtaactga tgaaaacaaa gatttttact ttatccaaaa agatggcttt acctttgcat
     6721 tatcaaagtc agaaggagag catcacattg gtgaaatggt taaaggattc gcctatacag
     6781 atatgcaaca aaaagcacgt cttacaacta aagaaacatt tgcgactcga gatcattatg
     6841 gttgggggac agttactgag gtacgtaaag acttgggagt atttcttgat actggtcttc
     6901 cagataagca agtggttgtt tctttggatg ttcttcctga attgaaagaa ctttggccta
     6961 aaaaaggtga tcgcctctac gtttgcttag atgttgataa aaaagatcgt ctctgggctc
     7021 tccctgcaga tcctgaagtg tttcaacgaa tggcaacccc tgcatataac aatatgcaaa
     7081 atcaaaattg gccagcaatc gtttatcgct taaaattgtc aggaactttt gtttatctac
     7141 cagaaaataa tatgctcggc tttattcacc ctagtgaacg ttacagtgaa cctcgtttag
     7201 ggcaagtgct ggatgcacga gttattggct tcagagaagt tgatagaacc ttaaatttat
     7261 cattaaagcc tcgttctttt gaaatgttag aaaatgacgc acaaatgatt ttaacgtatt
     7321 tagaaagtaa cggaggattc atgactttaa atgataaatc ttctccagag gaaattaaag
     7381 ctacttttgg aatttccaaa ggtcagttta aaaaagcact tggaggactc atgaaagcta
     7441 aaaagatcaa acaagatcag ctaggtacag aattattata aacttttgaa cttttaaagt
     7501 tttctcacga ggtcgtttta gttatagcta gaattaatgg ctaattatag aaaaaagctg
     7561 agaggaaatt ttggcttttt ttgttataat gtatctatta aaaagaatgg agaacgatat
     7621 ggaacgtgcg atttttgctg gaggttgttt ttggtgtatg gtacaaccat ttgaagaatt
     7681 agatggtatt gaatctgttt tatctggata cactggagga catgttgaga atccaactta
     7741 taaagaagtt tgtagtaaaa ctactggaca tacagaagct gttgaaatta tttttaatcc
     7801 tgaaaaaatc tcttatgcag atttagttga gttatattgg gcacaaacag atccaacaga
     7861 cgcttttggt cagtttgaag atcgtgggga taattatcgt cctgtcatct tttatgagaa
     7921 tgaagagcaa cgtcagattg ctcaaaaatc aaaagataag ctacaagctt cgggtagatt
     7981 tgatagacca attgtaacca gcattgagcc tgctgatact ttttacccag cagaagacta
     8041 tcatcaagca ttctatcgga ctaaccctgc tcgttacgcc ttgagtagtg ccagaagaca
     8101 tgcatttcta gaggagaact ggcattgaga aaatcatttt acagttggtt gatgacacaa
     8161 agaaatccaa aatcaaatga accagtagca atcctagcag attacgcttt tgatgaaacc
     8221 acttttccaa aacatagttc ggattttgaa acagttagtc gctatttaga agatgaggcc
     8281 agtttttcct ttaatttaac agattttgat gatatttggg aagattatct caatcattaa
     8341 caataatata gagattgtat tttcaatctc ttttttaggt tatagaaatt ggacacattg
     8401 tctaagactg tctgaaaagc aggtttttta atggcaatcg ttttcacaaa ggagtagaat
     8461 atagtcatct aaaatatagc cctagggagg ttgtttatga gatatacaaa tggaaatttt
     8521 gaagcctttg caagacctcg aaaacctgaa ggtgtggata aaaaatccgc ttatattgtt
     8581 ggttctggtt tagcaggatt agctgccgct gtctttttaa tacgtgacgg tcaaatggat
     8641 ggtcaacgta ttcatatttt tgaagaacta cctctttctg gaggatcact tgacggtgtc
     8701 aaacgacctg atatcggttt tgtaacgcgt ggtggtcgtg aaatggaaaa tcacttcgaa
     8761 tgtatgtggg atatgtaccg ttccatcccc tctctcgaag ttccagatgc ttcttatcta
     8821 gatgaatttt attggcttga caaggatgat cccaattcat ctaactgtcg cctcattcat
     8881 aaacagggga atcgcttaga atctgatggt gattttacac tcggaacaca ttccaaagag
     8941 ttagttaagc tagtcatgga gactgaagag tctttaggtg ctaagacgat tgaagaagtt
     9001 ttttcaaaag aattttttga aagtaatttt tggacttatt gggctactat gtttgccttt
     9061 gagaaatggc attcagcgat tgaaatgcgt cgatatgcta tgcgctttat ccatcatatt
     9121 ggtggtctgc ctgatttcac ttcattaaaa tttaataaat ataatcaata tgattctatg
     9181 gtgaaaccaa tcatcagtta tttagagtct cataatgtag atgttcaatt tgatagcaag
     9241 gtaactaata tctccgtaga ctttaagaac ggtcagaagc ttgcaaaagc cattcatctc
     9301 acagttggtg gtgaagcaaa aacaattgat cttactccaa atgattttgt ttttgtaacg
     9361 aatggttcta tcactgaaag tacgaactat ggtagtcatg atactgtagc taaacctaat
     9421 accgatttgg gaggttcttg gaatctttgg gaaaacttag ctgctcaatc tgatgaattt
     9481 ggacatccta aagtcttcta caaagatatt ccaaaagagt catggtttgt ttctgctact
     9541 gtaacaataa aagatccagc tattgaacct tatatagagc gtttaacaca tcgtgacctc
     9601 catgatggta aagtaaatac cggaggcatt gttacagtta cagattctaa ttggatgatg
     9661 agttttgcta tccatcgcca accgcatttc aaagagcaaa aggaaaatga aacaattgtt
     9721 tggatttatg gactctattc aaatgtggaa ggtaattata ttaaaaaacc aattgaagaa
     9781 tgtactggtc gagaaatcac agaagagtgg ttatatcact taggtgttcc tgaaatgaag
     9841 attcatgatt tatctgataa acaatatgta agtacagttc ctgtttatat gccttacatt
     9901 actagttatt tcatgcctcg agttaaaggt gaccgaccag atgttatccc acaaggatct
     9961 gttaacctag ccttcattgg taattttgca gaatctccat ccagagatac agtatttaca
    10021 acggaatatt ctattagaac tgctatggaa gctgtttata ctttcttaaa tattgaacgt
    10081 ggtgttccag aagtctttaa ttcagcattt gatattcgtg tcttgttgca atctttatac
    10141 tatctcaatg ataagaaatc tgttgaagat atggatttac caatacctgc cttaatgcgt
    10201 aaagtaggca tgaaaaaaat tagaggaact tatttagaag aattacttcg agaagctcat
    10261 cttttataat gtaaaataaa atcgagaaaa cttgtttttc tcgatttttt agttttacaa
    10321 cgactaaaat caccaaagaa aatgaaattt tgttataatg tagtcactta ctgtacgaaa
    10381 aggaagaata atttgcaaga atactctatc gaaattactt tacaacatcc agatgatatg
    10441 atgtcactct ttggcagcaa tgagcgccat ttaaaattaa tcgaagaaaa tttagatgtt
    10501 atcattcatg ctagaactga acgtgttcag gtattaggtg actctgaaga agcagtagaa
    10561 accgctcggt taaccattga agcattatta gtgcttgtta accgcgggat gactgtgaat
    10621 acttcagatg ttgtaacagc cttatccatg gcacaaaatg gttcaattga taaattcgtt
    10681 gccctttatg aggaagaaat tattaaggat agctatggta agcctatccg tgttaagact
    10741 ttagggcaaa aaatatacgt tgatagcgtt aaaaatcatg atgttgtttt tggtattggg
    10801 cctgctggaa cgggaaaaac ttttttagca gtcactctag cagtaacggc actaaagcgt
    10861 ggtcaagtta aacgaataat cttaacgaga ccagcggttg aagctggtga aagccttggt
    10921 ttcttgcctg gagatctcaa agagaaagtt gatccttatt tgagaccagt ttatgatgcc
    10981 ctatatcaaa ttttaggtaa agagcaaaca agtcgcttaa tggagcgtga aatcatcgaa
    11041 attgctccat tggcttacat gcgtggtcgt actttagatg atgcatttgt catattagat
    11101 gaagcgcaaa acaccactat aatgcaaatg aaaatgttcc taacacgtct tggcttcaat
    11161 tctaaaatga ttgtaaatgg cgatgttagc caaattgact taccaaagaa tgttaaatct
    11221 ggtttgattg atgctgttga gaaattacga aatatcaaaa agattgactt tattcattta
    11281 tcagcaaagg atgttgtaag acaccctgtc gttgctgaaa taataaatgc ttattcagat
    11341 tcagaaagta gtcacaagct acaatctgat aaggactgat gtttgccact aggagacgtt
    11401 gttagagagt gtgtcaatat gtaaaaaacg cctaatgctt taggcgtttt tatgatggtg
    11461 tacttcaact taacctataa agtgttgaaa attttcttta agcatgacgt cgagatgcat
    11521 tattatatcg tttaatgttt ctttaacagt gtgttcgttg gagttgtgat gacatagttt
    11581 aatgatattt tcttcgaaat gtttaggaag caatgtagca ttcttcttag cataggaaat
    11641 cattcttttt tcgccaggat gtgtcaactt attaagtgca aaaataatat caaagtagga
    11701 atctaagaaa gcagtagttc tatggtgagt actaacaaag tcttggcgtt taagggcttt
    11761 tataatttgt ttatcgtaag agggaagctt gccagttaat aagttacgat tttgaatgat
    11821 aatttgtttt tgtaactgac tgggataaga aacttcgaat ctctctttga gtgattgtaa
    11881 ttgattttca ggatcataga gtatttggca attgatgagg ttatgccaaa aacaagtagt
    11941 gtatccaatt cgagaattgt ggtgttcaac gacatcttct aagtctgata aaaagttatc
    12001 tatgttacga tagagaatat caatatcagt tccgtcgttt aaagtaccat tatcttcaag
    12061 ctcccagtaa tgattgttaa gttcaatgta atgacagtgc ttgttaagga tacgtttacg
    12121 actagtgata tcaggagttg ctgcacaata aacatacaaa tcataatcgg aagattggtc
    12181 aaaactgtca ccagagcgtg agcctccgag tgctatagca acaacatttg gaagtttaga
    12241 gaaatcttgc cagagttttg tgaaatggtc cataaatgct ctcctaaaaa tgtaaaatat
    12301 tgatgtgtct ccattctaca gggctatctc tttttagtca ataattaata gtaagttaat
    12361 aaaagaggta acggttctaa tattcaagaa gtgtatatga gtgatttgaa aaggagatat
    12421 tttatgctac aatacgtaca tattagttta aacaaggtga tagaatgaaa caagattata
    12481 tttcctacat taggtcaaaa gttggacatg agactatctt tttaacatat tctggtggta
    12541 ttttgacaga tggtaaggga cgtgtcttac tccaattaag agctgataag aattcttggg
    12601 gaattattgg tggatgtatg gagttaggtg agtcatcagt agatacattg aaaagagaat
    12661 ttttcgaaga aactggattg agagtggaac caatcagact gttaaatgtt tatactaact
    12721 ttcaagactc ttatcccaat ggcgataaag cacaaaccgt cggttttatt tatgaagtaa
    12781 gctgtccaaa accagttaat atagaaggtt ttcataatga ggaaacattg caattggact
    12841 atttttctaa agaagacgtc aaaaatatta cgatcgtaaa tgaacaacac caattaattt
    12901 tagatgaata tttttcacaa acattccaaa tggggcgtta aaaggatggg aaatactagg
    12961 tgtgacttta aagaacttct aacttttatt gatatgttgt taggcattct tgtagaaaca
    13021 aatgtta
//
LOCUS       DBJYSH010000057        12931 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.057, whole genome shotgun sequence.
ACCESSION   DBJYSH010000057 DBJYSH010000000
VERSION     DBJYSH010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 12931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12931
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.057"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            78..923
                     /locus_tag="ACUBV3_001237"
     CDS             78..923
                     /locus_tag="ACUBV3_001237"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HHU3847741.1"
                     /translation="MLSVGLVLEGGGMRGLYTAGVLDAFLDAGIKIDGIVSVSAGALF
                     GVNFVSRQRERALRYNKKYLSHPKYMSLRSWFRTGNFVNKDFTYYEVPMKLDVFDDEA
                     FKKSSIDFYVVATEMTSGKPEYFKIDSVFEQMEILRASSALPVVSKMVDWQGKKYLDG
                     GLSDSIPVDFARGLGFDKLIVVMTRPLNYQKKPSSGRLYKTLYRKYPNFVKTASNRYQ
                     QYNNSLEKVMSLEKTGDLFAIRPSKSLVIGRLEKNPDKLDSIYQLGMKDAKSVMPELN
                     SYLMK"
     gene            complement(966..2213)
                     /locus_tag="ACUBV3_001238"
     CDS             complement(966..2213)
                     /locus_tag="ACUBV3_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HHU3847742.1"
                     /translation="MSPLNINKNNWRALIAAIVASGTDDLNIMFLAFSMSTIITDLHL
                     SAAQAGWIGTITNLGMLVGGLIFGLLADRYNKFKVFKWTILIFSIATGLVFFTTNLSY
                     LYIMRFIAGIGVGGEYGIAIAIMAGIVPTNKMGRISSLNGIAGQVGSISSALLAGWLA
                     PALGWRGLFLFGLLPIVLVLWMQFAVDDKDILDQYNTDADDEPLDISIKALFDTPVLA
                     TQSLALMVMTTVQIAGYFGMMNWLPTIIQTNLNVSVKNSSLWMIATILGMCLGMLVFG
                     QLLDKFGPRLVYGCFLLSSAICVYLFQFATTMPSMIIGGAVVGFFVNGMFAGYGAMIT
                     RLYPHHIRSTANNLILNVGRAIGGFSSVIIGMILDVSNVSMVMLFLASLYIVSFLSML
                     SIKQLKRQKYHTNLTQLDVKPTD"
     gene            2590..5091
                     /gene="leuS"
                     /locus_tag="ACUBV3_001239"
     CDS             2590..5091
                     /gene="leuS"
                     /locus_tag="ACUBV3_001239"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HHU3847743.1"
                     /translation="MTFYNHKEIEPKWQAFWADNHTFKTGTDASKPKFYALDMFPYPS
                     GAGLHVGHPEGYTATDILSRFKRAQGHNVLHPMGWDAFGLPAEQYAMDTGNDPAEFTA
                     ENIANFKRQINALGFSYDWDREVNTTDPNYYKWTQWIFTKLYEKGLAYEAEVPVNWVE
                     ELGTAIANEEVLPDGTSERGGYPVVRKPMRQWMLKITAYAERLLEDLEEVDWPESIKD
                     MQRNWIGKSTGANVTFKVKDTDKDFTVFTTRPDTLFGATYAVLAPEHALVDAITTADQ
                     AEAVAEYKRQASLKSDLARTDLAKEKTGVWTGAYAINPVNGKEIPVWIADYVLASYGT
                     GAIMAVPAHDERDWEFAKQFNLDIIPVLEGGNVEEAAFTEDGLHINSDFLDGLDKAAA
                     IAKMVEWLEAEGVGNEKVTYRLRDWLFSRQRYWGEPIPIIHWEDGTSTAVPESELPLV
                     LPVTKDIRPSGTGESPLANLTDWLEVTREDGVKGRRETNTMPQWAGSSWYYLRYIDPH
                     NTEKLADEELLKQWLPVDIYVGGAEHAVLHLLYARFWHKVLYDLGVVPTKEPFQKLFN
                     QGMILGTSYRDSRGALVATDKVEKRDGSFFHVETGEELEQAPAKMSKSLKNVVNPDDV
                     VEQYGADTLRVYEMFMGPLDASIAWSEEGLEGSRKFLDRVYRLITTKEITEENSGALD
                     KVYNETVKAVTEQVDQMKFNTAIAQLMVFVNAANKEDKLFSDYAKGFVQLIAPFAPHL
                     GEELWQVLTASGQSISYVPWPSYDESKLVENEIEIVVQIKGKVKAKLVVAKDLSREEL
                     QDLALANEKVQAEIAGKDIIKVIAVPNKLVNIVVK"
     gene            complement(5178..5786)
                     /locus_tag="ACUBV3_001240"
     CDS             complement(5178..5786)
                     /locus_tag="ACUBV3_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000903267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome assembly protein"
                     /protein_id="HHU3847744.1"
                     /translation="MLHSKKIHSLSLIAVLSLATYTSLQPNHVTAEQSQKTSTVHMSQ
                     KTIEHKLKVADKEAAPLYAKIDHIQRHIEVKKAKDLKVIELYINKDINQLEKQNKRLL
                     TKFYTSIDNQTWDSTSEVKKLIDKTNLSTNEKDRLKLYFEQRAYLETRLNDRYQKFDN
                     SIENQNKELKILTSKIEKIYQKHGITKEVLKTYYAKKTVRAD"
     gene            complement(5879..7267)
                     /locus_tag="ACUBV3_001241"
     CDS             complement(5879..7267)
                     /locus_tag="ACUBV3_001241"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000730412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HHU3847745.1"
                     /translation="MKKKSLISNLILINSITLFLGFYLIYFSVNYVIKDYVHQMTRNS
                     MQANFTILDSIYNNKKIPDENMVNQDSIFVWSYYAIFDQHYRMKYGTDNHKKESHDII
                     RYLTQKRLWQISKEKDGMFVTIKKKTYYVMTKDYSGILVDGSIKKVPKAQSQLFHVIN
                     FSDITYTQHLITKINHFLIVILVLTYIPMLFIMRKTFTGIRESIQSVQTYISSLWKNQ
                     GNHQSSQKEIVFSDFDPLLLESQEMANRIYQAEESQRNFFQNASHELRTPLMSIQGYT
                     EGVQEGIIDAELAHSVILQESKKMKQLVDDIILLSKLDSNLSDQKDEFSLNELLNSII
                     AYFKPLANKQKISITYRPDKHEKLLKGNEELIQRAINNILSNALRYAVSHIEISYTNQ
                     KLTISNDGPAISKEDLPYIFDRFYKGHGGQTGIGLAMTKEIIKQHHGNIIAESDSTST
                     TFTIYFNCHKIA"
     gene            complement(7264..7950)
                     /locus_tag="ACUBV3_001242"
     CDS             complement(7264..7950)
                     /locus_tag="ACUBV3_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HHU3847746.1"
                     /translation="MTKIIYFADDEKNIRDLVVPFLEHDGFTVRAFETGDLLLEAYKN
                     QKPDLVILDIMMPGTNGLDVMKSIRQYDNIPIIMLTARDSDVDFITAFNLGTDDYFTK
                     PFSPIKLSLHVKALFKRLDEKAIKNDTQYQFLDLTLDTEKRIALLSNEEMPLTKTEFD
                     FLLVLIEKPETAFSRETLLNRIWGFDDIESRAVDDTIKRLRKKFKQYHSQVSIKTVWG
                     YGFKLIKEDK"
     gene            complement(8183..12895)
                     /locus_tag="ACUBV3_001243"
     CDS             complement(8183..12895)
                     /locus_tag="ACUBV3_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HHU3847747.1"
                     /translation="MNTKQRFSIRKYKLGAVSVLLGTLFFLGGITNVAADSVINKPSD
                     IAVEQQVKDSPTSIANETPTNNTSSALASTAQDNLVTKANNSPTETQPVAESHSQATE
                     TFSPVANQPVESTQEVSKTPLTKQNLAVKSTPAISKETPQNIDSNKIITVPKVWNTGY
                     KGEGTVVAIIDSGLDINHDALQLNDSTKAKYQNEQQMNAAKAKAGINYGKWYNNKVIF
                     GHNYVDVNTELKEVKSTSHGMHVTSIATANPSKKDTNELIYGVAPEAQVMFMRVFSDE
                     KRGTGPALYVKAIEDAVKLGADSINLSLGGANGSLVNADDRLIKALEMARLAGVSVVI
                     AAGNDGTFGSGASKPSALYPDYGLVGSPSTAREAISVASYNNTTLVNKVFNIIGLENN
                     RNLNNGLAAYADPKVSDKTFEVGKQYDYVFVGKGNDNDYKDKTLNGKIALIERGDITF
                     TKKVVNAINHGAVGAIIFNNKAGEANLTMSLDPEASAIPAIFTQKEFGDVLAKNNYKI
                     VFNNIKNKQANPNAGVLSDFSSWGLTADGQLKPDLSAPGGSIYAAINDNEYDMMSGTS
                     MASPHVAGATALVKQYLLKEHPELKKGDIERTVKYLLMSTAKAHLNKDTGAYTSPRQQ
                     GAGIIDVAAAVQTGLYLTGGENNYGSVTLGNIKDKISFDVTVHNINKVAKDLHYTTYL
                     NTDQVKDGFVTLAPQQLGTFTGKTIRIEPGQTQTITIDIDVSKYHDMLKKVMPNGYFL
                     EGYVRFTDPVDGGEVLSIPYVGFKGEFQNLEVLEKSIYKLVANKEKGFYFQPKQTNEV
                     PGSEDYTALMTTSSEPIYSTDGTSPIQLKALGSYKSIDGKWILQLDQKGQPHLAISPN
                     DDQNQDAVAVKGVFLRNFNNLRAKVYRADDVNLQKPLWVSAPQAGDKNYYSGNTENPK
                     STFLYDTEWKGTTTDGIPLEDGKYKYVLTYYSDVPGSKPQQMVFDITLDRQAPTLTTA
                     TYDKDRRIFKARPAVEHGESGIFREQVFYLKKDKDGHYNSVLRQQGEDGILVEDNKVF
                     IKQEKDGSFILPKEVNDFSHVYYTVEDYAGNLVSAKLEDLINIGNKNGLVNVKVFSPE
                     LNSNVDIDFSYSVKDDKGNIIKKQHHGKDLNLLKLPFGTYTFDLFLYDEERANLISPK
                     SVTVTISEKDSLKDVLFKVNLLKKAALLVEFDKLLPKGATVQLVTKTNTVVDLPKATY
                     SPTDYGKNIPVGDYRLNVTLPSGYSTLENLDDLLVSVKEDQVNLTKLTLINKAPLINA
                     LAEQTDIITQPVFYNAGTHLKNNYLANLEKAQTLIKNRVEQTSIDNAIAALRESRQAL
                     NGKETDTSLLAKAILAETEIKGNYQFVNASPLSQSTYINQVQLAKNLLQKPNVTQSEV
                     DKALENLDIAKNQLNGHETDYSGLHHMIIKANVLKQTSSKYQNASQFAKENYNNLIKK
                     AELLLSNRQATQAQVEELLNQIKATEQELDGRDRVSSAENYSQSLNDNDSLNTTPINP
                     PNQPQALIFKKGMTKESEVAQKRVLGVTSQTDNQKVKTNKLPKTGESTPKITYTILLF
                     SLSMLGLATIKLKSIKRE"
BASE COUNT         3828 a         2299 c         2511 g         4293 t
ORIGIN      
        1 aacatattct ttttcagaaa aaacagctat attttgctat actttaatca aatatcaata
       61 ttagaaagtg attgcctatg ttgtctgttg gtttagtttt agagggtggc ggaatgagag
      121 gtctttatac tgctggagtt ttagatgctt ttctagatgc aggaataaaa atagatggta
      181 tcgtatctgt ctctgctggt gcattgtttg gtgttaattt tgtatctaga caacgagaga
      241 gggctttgcg atacaataaa aagtatttat cccaccctaa atatatgagt ctaaggtcat
      301 ggtttcgaac agggaatttt gttaataaag atttcaccta ttatgaagtt cctatgaaat
      361 tggatgtatt tgacgatgaa gcatttaaaa aatcaagtat tgatttttac gtagttgcta
      421 cagagatgac atctggtaaa cctgaatatt ttaaaattga tagtgttttt gaacaaatgg
      481 aaattttacg tgctagttca gcattaccag tagtctcaaa gatggttgat tggcagggga
      541 aaaagtactt agatggtggt ttatctgata gtattcccgt tgattttgcc cgtggtttag
      601 gatttgacaa gttgattgtt gtgatgacta ggccgctcaa ttatcagaaa aagccttcaa
      661 gtggacgatt gtataaaact ctgtatagga aatatcctaa ttttgtaaag acagcctcga
      721 atcggtacca acagtataat aatagtcttg aaaaggtcat gagccttgaa aaaacaggcg
      781 atctatttgc aattagaccg agtaagagct tggttattgg ccgcttagag aagaatccgg
      841 ataaacttga tagtatttat cagcttggta tgaaagatgc taaaagtgtg atgcctgagc
      901 tgaatagtta tctaatgaaa taaagaaaac agtatttctc aattgaggaa tactgttttg
      961 taatatcagt cagttggttt gacatctaat tgtgttaaat tagtgtgata tttttgacgt
     1021 tttaattgct taatgcttag cattgataaa aaactaacga tatagagact tgctaagaaa
     1081 agcatgacca tagagacatt tgaaacgtct agaatcattc cgatgataac agatgaaaag
     1141 ccacctattg cacgaccaac atttaagata agattgttag ctgttgaacg aatatgatgt
     1201 ggatagagac gtgtaatcat ggcaccatag cctgcaaaca taccattaac aaagaatccg
     1261 acaactgccc cgcctataat catagaaggc attgttgttg cgaattgaaa gagatagaca
     1321 caaattgcag atgatagaag gaaacacccg tatactaaac gcggtccaaa tttatctaac
     1381 aattgaccaa atactagcat gccaaggcac attccaagaa tggttgcaat catccataat
     1441 gatgaattct tgacagaaac gtttaagttg gtttggataa tagttggtaa ccagttcatc
     1501 atgccaaagt atccggcaat ctgaacagtt gtcataacca ttagtgctag gctctgtgtg
     1561 gctaatacag gagtatcaaa taaagctttt atactgatat ctagaggttc atcatctgcg
     1621 tctgtattat attgatctaa gatatcttta tcatcaacag caaattgcat ccataggacg
     1681 agaactatag ggagaaggcc aaagagaaaa agtccccgcc aaccgagtgc tggagctagc
     1741 catcctgcta agagtgctga gctaatagat ccaacttgac cagcgatacc atttagtgaa
     1801 gaaatccggc ccattttatt agttgggaca attcctgcca taatggcaat agcgatacca
     1861 tattcgccac caacgccaat tcctgcaatg aagcgcatga tatagagata tgataaatta
     1921 gtagtgaaga aaactaaacc agttgcgatt gaaaagatta gaatcgtcca tttgaaaact
     1981 ttaaatttat tgtagcgatc agctaataaa ccaaagatta gtcctcccac gagcattccc
     2041 aagttggtaa ttgtcccaat ccaaccagcc tgtgccgcag ataaatgaag atcagtaatg
     2101 atagttgaca tagaaaatgc aaggaacatg atatttaaat catcggttcc ggatgcgaca
     2161 atggcagcga ttagggcgcg ccaattattc ttattgatat ttaatggtga cataagtctc
     2221 ctcctttaat gatggctcac agaccaccgt tattaaatac tgtcaataga aaaaattcta
     2281 aaacagagat gtcatagctg cttcaactca ttataatgta aaagaaaaaa ctcaagctgc
     2341 tacccttgag ttctataaac gctcgcaaaa atagtaggat ttctgtatag aatcctattt
     2401 tggtcgctta tatgtctcac aggacacggt taaagcatta tgtcatttgg aaatagtata
     2461 gagtaagttt agtttattgt caaggataag tggggagaga acctgctgtt gagggtattt
     2521 tcaccttttt tattgctatt tttcgtgtta taataagaag actatacttt tttgacatgg
     2581 agaagtgcga tgacattcta taatcacaaa gaaatcgaac ccaaatggca agctttttgg
     2641 gcagataatc atacctttaa aacaggaaca gacgcatcaa aacctaagtt ttatgcccta
     2701 gatatgttcc catacccatc tggagctggt ctccacgttg gtcacccaga aggctataca
     2761 gcaacggata ttctcagccg tttcaaacgt gcccaaggtc acaatgtcct ccacccaatg
     2821 ggttgggatg cttttggact tcctgctgag cagtacgcta tggatactgg taatgaccca
     2881 gctgagttta cagctgaaaa catcgctaac ttcaaacgcc agattaatgc tcttggtttc
     2941 tcatacgact gggatcgtga agttaacaca actgacccta actactacaa gtggacacag
     3001 tggatcttta ccaagcttta tgaaaaaggt ctagcttacg aagctgaagt tccagttaac
     3061 tgggttgaag aattgggaac agctatcgcc aacgaagagg ttcttccaga tggtacatca
     3121 gaacgtggtg gctacccagt tgtcagaaaa ccaatgcgcc aatggatgct caaaatcact
     3181 gcctatgcag agcgcttgct ggaagacttg gaagaagttg actggccaga gtcaatcaag
     3241 gacatgcaac gcaactggat tggtaaatca actggtgcta atgtaacctt caaggttaag
     3301 gatacagata aggacttcac tgtctttaca acacgtcctg acaccctttt tggtgcgact
     3361 tacgcagttc ttgctcctga gcatgccttg gttgatgcta ttacaacagc tgaccaagca
     3421 gaagccgttg ccgaatacaa acgtcaagcg agtctcaaat cagaccttgc tcgtacagac
     3481 cttgccaaag aaaaaactgg tgtctggaca ggtgcctatg ccattaaccc agttaatggt
     3541 aaagaaattc cagtttggat tgcggactat gtccttgcta gctacggtac aggtgctatc
     3601 atggcagttc cagctcacga tgaacgtgac tgggaattcg caaaacaatt caacctagac
     3661 atcattccag ttcttgaagg tgggaatgtt gaagaggctg cctttacaga agacggtctt
     3721 cacatcaact cagacttcct agatggtctc gacaaagcgg cagctattgc caaaatggtc
     3781 gagtggcttg aagcagaagg tgttggtaat gagaaagtta cttaccgtct tcgtgactgg
     3841 ctcttcagcc gtcagcgtta ttggggtgag ccaatcccaa tcatccactg ggaagatgga
     3901 acatcaactg cggttcctga gagcgaattg ccactagttc ttccagttac taaggatatc
     3961 cgtccttcag gtactggtga gagcccactt gctaacttga cagactggtt ggaagtcact
     4021 cgtgaagatg gcgttaaagg tcgtcgtgag accaacacca tgccacaatg ggcaggttca
     4081 agctggtact atctccgcta catcgatcca cacaacactg aaaaactagc tgacgaggaa
     4141 ctcctcaaac aatggttgcc agttgatatc tatgtcggtg gtgcagagca tgccgtgctt
     4201 caccttctct atgctcgctt ctggcacaag gttctttatg atctcggtgt cgtacctacc
     4261 aaagagccat tccaaaaact ctttaaccaa gggatgattt tgggaacaag ctaccgcgac
     4321 agccgtggtg ctcttgtggc aactgacaag gttgaaaaac gtgatggcag cttcttccat
     4381 gtggaaactg gtgaagagct tgaacaagca cctgctaaga tgtctaaatc acttaagaac
     4441 gttgtcaatc cagatgatgt tgttgagcag tacggtgctg acacccttcg tgtttacgag
     4501 atgttcatgg gaccactcga tgcttcaatc gcttggtctg aagaaggtct tgaaggctca
     4561 cgtaagttct tggaccgtgt ttaccgtttg atcacgacta aagagatcac tgaggaaaat
     4621 agcggagctc ttgacaaggt ttacaacgaa actgttaagg cagttactga gcaagttgac
     4681 caaatgaaat tcaacacagc tatcgctcaa ctcatggtct ttgtcaatgc agccaacaag
     4741 gaagacaaac tctttagcga ctatgccaaa ggctttgttc aattgattgc accattcgca
     4801 ccgcacttag gtgaagaact ctggcaagtc ttgacagcct caggacagtc aatctcttac
     4861 gtgccatggc caagctatga cgaaagcaaa ttggttgaaa acgaaatcga aatcgttgtc
     4921 caaatcaaag gtaaggtcaa agctaaactc gtcgttgcta aagacctttc acgcgaagaa
     4981 ttgcaagatc ttgctcttgc taacgaaaaa gtccaagccg aaatcgcagg caaagacatc
     5041 atcaaagtca tcgcagtacc aaacaaactc gttaatattg tggtgaaata agataaagat
     5101 tttactagaa atagtgatat ttacgcgtat aaaatcccaa aagtttaact tttgggattt
     5161 ttctattttt agctaaatca gtcagctcgt actgtttttt tagcatagta agtttttaat
     5221 acctcttttg taataccatg tttttgatag attttttcta tttttgacgt taatattttt
     5281 agttctttat tttggttttc aatagagtta tcaaattttt gatagcggtc gttcaacctt
     5341 gtctcaaggt aagcacgttg ttcaaaatat aattttaatc tatctttttc gttagtggat
     5401 aggtttgtct tatcaatcaa ttttttgact tcacttgtgc tatcccatgt ttgattatca
     5461 atagaagtat agaatttagt tagtagacgt ttattttgct tctctagttg gttgatatct
     5521 ttgttaatat acaattcaat aacttttaaa tcttttgctt ttttgacttc aatatgtcgt
     5581 tggatatggt cgattttagc gtagagagga gcagcttctt tatctgcaac ttttaactta
     5641 tgttcaatag ttttttgact catatgaaca gttgatgttt tttgtgattg ttcagccgtt
     5701 acatgatttg gttgtaaact cgtatatgtt gctaaagaga gaacggcaat aagcgataag
     5761 gaatgtattt ttttagaatg taacatataa aaactcactt tcttttagaa taactttttg
     5821 atctataggt attataggtt aaaaaagcaa taacttcttt gaatttttgt aggaaaagtt
     5881 aggcaatttt gtggcaattg aaataaatcg taaaagttgt tgaggtcgaa tcactctcag
     5941 cgatgatatt accatgatgt tgcttgataa tttctttagt cattgcaagt ccaatacctg
     6001 tttggccgcc atgtccttta tagaagcgat cgaaaatata aggcaagtct tcttttgaaa
     6061 tggctggtcc gtcgtttgag atagttagtt tttgatttgt gtaagagatt tcaatatgag
     6121 aaacagcata gcgtagtgcg ttactaagga tattattgat agctcgttga atcaactctt
     6181 cgttaccttt tagtaacttt tcatgcttgt ctggtctata agtaatacta atcttttgtt
     6241 tattagctag gggtttgaaa taagcaataa tactattaag taattcattt aaagaaaatt
     6301 catctttttg atcacttaag ttactatcta atttagagag taagataata tcgtctacga
     6361 gctgtttcat ttttttgctc tcttgcagaa taacagagtg tgcgagctcg gcatcaataa
     6421 taccctcttg aactccctca gtatatcctt ggatggacat taaaggtgtc cgtaactcgt
     6481 gggaggcatt ttgaaagaag tttctttggc tctcttctgc ttggtagatt cgatttgcca
     6541 tttcttggct ctcaagtaga agaggatcga aatcagaaaa gacaatttct ttttgactag
     6601 attgatgatt gccttgattt ttccaaaggc tggaaatata agtttgtact gattgaatag
     6661 attctctaat tccagtgaaa gtcttcctca taataaatag catgggaata taggttagta
     6721 ctaagataac tataagaaaa tgattaattt tagtaattag atgctgggtg taagtaatgt
     6781 ctgaaaagtt aatcacatga aaaagttgcg actgtgcttt aggaactttt tttatgctac
     6841 catcaacaag gataccagag tagtctttgg tcattacata ataggttttt ttcttgatgg
     6901 ttacaaacat accatctttt tccttagaaa tttgccacaa tcttttttgg gtaagatagc
     6961 gaataatatc atggctctct tttttatgat tatcagtgcc atatttcata cgataatgct
     7021 ggtcaaaaat agcatagtaa gaccaaacaa agatgctatc ttggttaacc atattttcat
     7081 ctggtatttt cttgttgtta tagatactat ctaaaatggt gaaattcgcc tgcatggaat
     7141 tccttgtcat ttgatggaca tagtctttaa tgacatagtt gacagaaaaa tagatgaggt
     7201 aaaaaccaag aaatagagtg atgctattaa taaggataag attgctaatt aaacttttct
     7261 ttttcatttg tcctctttga ttaatttgaa gccgtaaccc cagacagttt tgatactaac
     7321 ctgactgtgg tattgcttaa attttttacg taatcgttta atagtgtcat caactgctct
     7381 gctttcaata tcatcaaatc cccaaatacg attaagcagt gtttccctag aaaaagcagt
     7441 ttcaggtttt tcaattaata ctaaaagaaa atcaaattct gtcttggtta agggcatttc
     7501 ttcatttgat aatagggcta ttcgcttttc ggtatccaga gttaaatcta ggaactgata
     7561 ctgagtgtca tttttgattg ctttttcatc aaggcgtttg aacaatgctt taacatgtag
     7621 actaagtttt ataggtgaaa aaggcttggt gaagtaatcg tcagttccta aattaaaagc
     7681 cgtaatgaaa tcaacatcag aatctctagc tgttaacatg ataataggaa tattatcata
     7741 ttggcggatg cttttcataa catctaaacc attagttcca ggcatcatta tgtctaatat
     7801 aactaaatcg ggtttttggt ttttataggc ttctaagaga aggtctccag tttcaaatgc
     7861 tcgaacagtg aaaccatcat gttctaaaaa aggaaccacc aagtcacgaa tattcttttc
     7921 atcatcagcg aaataaatta tttttgtcat gtatacctca atacccatag ttataatttg
     7981 acttttagct agctgtttat gtcatatcaa atataacaaa aaaagagttt ttttcccatt
     8041 ctttgaagtg ttatggtgtt ataaaccaag atactgcaga gaaaaggtct ttaggaacat
     8101 cattttaagc tcctcaaaaa ggcaccagtc actaactatt aatagctagt aattgatgcc
     8161 ttgctctatt ttttaaagta ttttattctc ttttgataga ctttagtttg attgttgcca
     8221 gacctagcat agagagacta aatagcaata ttgtataggt tattttagga gtgctttcgc
     8281 ctgttttagg aagcttgttt gtctttacct tttgattatc ggtttggcta gtcaccccta
     8341 agacacgctt ctgagcaacc tcactttctt tagtcatgcc ttttttgaat atcaacgcct
     8401 ggggctgatt tggcggattg ataggtgtgg tattgagaga gtcattatca ttgagtgatt
     8461 gactataatt ctctgcggaa gaaactctat cacggccatc aagctcttgt tcggttgctt
     8521 ttatttggtt taataactct tcaacttgag cttgtgtagc ttgtctattg gaaagcagca
     8581 attctgcttt cttgataagg ttattataat tttctttagc aaattgactg gcgttctgat
     8641 atttagatga tgtttgtttc agaacgtttg ctttaattat catatggtgt aaaccagagt
     8701 aatcagtttc atgaccattt aattgatttt tagcaatatc aagattttct aaggctttgt
     8761 ctacttctga ttgagtgacg ttaggttttt gtagaaggtt tttcgccaat tggacttgat
     8821 tgatataagt tgattggctt aatggactag cattaacaaa ttgatagttt cccttgattt
     8881 ctgtttcagc taaaatagct tttgccagta aagaagtatc tgtttcttta ccgttaagag
     8941 cttggcgact ttctctcaaa gcagcaatag cattatcaat acttgtttgt tccactctat
     9001 ttttaattaa agtttgtgcc ttttcaagat tagctaggta attatttttt aagtgagttc
     9061 cagcattata aaacacaggc tgggtaataa tatcagtttg ttctgctagg gcattaatca
     9121 gaggagcttt attaatcagc gtcaattttg ttaggtttac ctgatcttct tttacggata
     9181 caagtaaatc atctaagttc tctaaagtgc tatacccact aggcagcgtt acgtttaaac
     9241 gatagtctcc tacaggtatg tttttaccat agtcagtagg agaataagtt gcttttggta
     9301 gatcaacaac agtatttgtc ttagtaacca actggactgt tgctcctttt ggtaaaagct
     9361 tgtcaaattc aacgagtaag gctgctttct tgagtaagtt aactttaaat aagacgtctt
     9421 taaggctatc tttttcagaa atagttacag tgacactttt gggactgatt agatttgctc
     9481 gttcctcatc gtataagaat agatcaaacg tataggtacc aaaaggcaat ttcaataaat
     9541 tgaggtcttt cccgtgatgt tgctttttga tgatattacc tttgtcatct ttgacagagt
     9601 aagagaaatc aatatcgaca ttactgttaa gctcagggct aaacactttg acgtttacta
     9661 aaccattttt attgccaata ttgatcaaat cttcgagttt tgctgatact agattgcctg
     9721 cataatcttc aacagtatag tagacatgag agaaatcgtt aacctcttta ggtagaataa
     9781 agctaccatc cttttcttgt ttgataaata ctttgttatc ttcaacaagg ataccgtctt
     9841 ctccttgttg acgtaagacg ctattataat gaccatcttt atcttttttt aagtaaaaaa
     9901 cttgttctct aaagatacca gattccccgt gttctactgc aggacgagct ttgaagatac
     9961 gtctatcttt gtcataagtt gctgttgtta gtgtaggagc ttgtctatcc aaagtgatat
    10021 caaacaccat ttgttgtggc ttagagccag ggacatcaga gtaataagtt aaaacgtatt
    10081 tgtattttcc atcttctaaa ggaataccat cagtagtggt tcctttccat tctgtgtcat
    10141 ataaaaatgt agattttgga ttttcagtat ttccgctgta gtaattttta tctcctgctt
    10201 ggggagcact tacccatagt ggtttttgta aattaacatc atctgcacga tagactttgg
    10261 ctcttaaatt attgaaatta cgtaagaaaa cacctttcac tgcaacggca tcttgatttt
    10321 ggtcatcatt aggtgaaatg gctagatgag gctggccttt ttgatctagt tgtaagatcc
    10381 attttccatc tatagactta tagcttccca aggctttcaa ttggatagga ctagtaccgt
    10441 ctgttgagta gataggctct gaacttgtag tcattaaggc agtataatct tctgaaccag
    10501 gaacttcgtt tgtttgtttt ggttggaaat aaaatccctt ttctttgtta gcaacaagct
    10561 tataaatgga tttttctaaa acttctaagt tttggaattc tcccttaaat ccaacataag
    10621 gaatactaag aacttcccca ccatcaacag ggtctgtaaa acgtacgtag ccttctagga
    10681 aatagccgtt tggcattact ttttttaaca tgtcatggta tttcgaaaca tctatatcaa
    10741 ttgtaatcgt ttgggtttgc cctggttcaa tccgtatcgt tttccctgta aatgtaccaa
    10801 gttgttgagg agccaatgtg acaaagccat ctttaacttg atcagtattt aaataggttg
    10861 tatagtgtaa atcttttgca actttattga tattatgaac agtaacatca aaggaaattt
    10921 tatctttaat atttcctaat gtaacgctgc catagttgtt ttccccacca gttaggtata
    10981 atcctgtctg aactgctgct gcgacatcga taatacctgc tccttgttgg cgtggtgagg
    11041 tgtaagcgcc tgtatctttg tttaggtgtg ctttagcagt actcataaga aggtatttga
    11101 cagttctttc aatgtcacct tttttaagtt ctggatgttc tttcaataag tattgtttaa
    11161 ctagagcagt agcaccagcg acatggggag aagccatact tgtcccactc atcatatcat
    11221 attcattatc attgatagcg gcgtaaatag agcctccagg agcagataag tcaggtttta
    11281 attgtccgtc tgctgttaac ccccagcttg aaaagtcaga taggacacct gcattagggt
    11341 tggcttgttt atttttgata ttgttaaata caattttata gttgttttta gctaaaacat
    11401 ctccaaactc tttttgggta aaaatagcag gaatagcact tgcttcagga tctaaactca
    11461 ttgttagatt agcttcccca gctttgttat taaagataat agctcccaca gcaccgtgat
    11521 taatagcatt gacgactttt tttgtaaaag taatatctcc acgttcaatt aaggcgattt
    11581 taccatttaa agttttgtcc ttataatcat tgtcgtttcc ttttcctacg aaaacataat
    11641 catattgttt ccctacttca aaggtcttat cactaacttt aggatctgca taagcagcta
    11701 atccgttgtt gagatttctg ttgttttcta atccgataat gttgaagact ttattaacca
    11761 gtgttgtatt attatatgat gctacagaaa tggcctcacg agctgttgat ggactaccaa
    11821 ctaaaccata atcaggataa agagcagaag gctttgatgc tccactccca aatgtaccgt
    11881 cgttacctgc tgctataaca acagaaacgc cagcgagtct agccatctct aaagctttta
    11941 taagtcggtc atcggcatta actaaagacc cattagctcc acctaaactt aaattaatgc
    12001 tgtcagcacc gagtttaacg gcatcttcaa tagctttaac ataaagggct ggtccagttc
    12061 ctcttttttc atcagagaag actctcataa acattacttg tgcttcagga gcaacaccat
    12121 agattaattc atttgtatct ttcttgctag gattagctgt tgcgatactg gttacgtgca
    12181 taccatgaga agtgcttttc acctctttta gctctgtatt gacatcaaca tagttgtgac
    12241 caaagattac tttgttgtta taccattttc catagtttat accagctttt gctttagcag
    12301 cattcatttg ctgttcgttt tggtattttg cttttgtcga atcatttaat tggagagcat
    12361 cgtgattgat atctagtcct gagtcaataa ttgctacaac agttccctct cctttgtagc
    12421 ctgtgttcca tactttgggg acagtgataa ttttattact atcaatgttt tgaggggttt
    12481 ctttagaaat agctggtgta gatttgactg ctaaattttg tttggttaaa ggagttttag
    12541 aaacttcttg agtgctttca accggttgat ttgcgactgg ggaaaatgtc tcggtggctt
    12601 gagagtgaga ctcagctact ggttgtgttt ctgttggact attattagcc tttgtaacaa
    12661 gattgtcttg agctgtcgac gcaagggctg atgacgtgtt gttagtaggt gtctcatttg
    12721 ctatgctcgt tggactgtct tttacttgct gttcaactgc aatatcagat ggcttattta
    12781 tgacagaatc agcagctaca tttgtgatac cacctaaaaa aaatagggtt cccaaaagta
    12841 cagatacggc acctaactta tatttccgga ttgaaaaacg ctgttttgtg ttcataaaaa
    12901 tctccttttc ttgtaacata ataattacaa a
//
LOCUS       DBJYSH010000058        12846 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.058, whole genome shotgun sequence.
ACCESSION   DBJYSH010000058 DBJYSH010000000
VERSION     DBJYSH010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 12846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12846
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.058"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            <1..302
                     /locus_tag="ACUBV3_001244"
     CDS             <1..302
                     /locus_tag="ACUBV3_001244"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HHU3847748.1"
                     /translation="YAKLRPNLKKVIISDENGFATTELLPIKVFGNISLDYIRYCMIS
                     PFYYFNIIQSVYGVKMPRVSSGFLNSTLLPLPPLTEQQRIVSKISQVFSVIESLV"
     gene            351..1148
                     /gene="psrA"
                     /locus_tag="ACUBV3_001245"
     CDS             351..1148
                     /gene="psrA"
                     /locus_tag="ACUBV3_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type DNA invertase PsrA"
                     /protein_id="HHU3847749.1"
                     /translation="MKDFELHLRKAGLAENTIRSYLYGVRFFLEHYELKIEDLFEYKG
                     YLLDNFKPKTVNLRLQGLNKYLVFIGHDDLKLKFVKIQQKPFLEDVISHADYLFLKRS
                     LKKDDNLKWHFVVWFLGATGARVSELIKLKVEHVEVGYFDIYSKGGKMRRLYIPKKLR
                     DSCQKWLDTENRQSGYLFLNKFSQPITARGIAQQLKNYAVKYQLNTKVVYPHSFRHLF
                     AKNFLAKYNDIALLADLMGHESIETTRIYLRKTATEQQAIIDRVVNW"
     gene            1248..1778
                     /locus_tag="ACUBV3_001246"
     CDS             1248..1778
                     /locus_tag="ACUBV3_001246"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HHU3847750.1"
                     /translation="MAEIPNSWSYVKFGSIVTFNIGKTPPRNEPTYWGNDIPWVSISD
                     MPSSGHITKTKECISHLAIKQTNIKIVPADTLLMSFKLSIGKVAILDVPASHNEAIIS
                     IFPYSDKKNIIRNYLMMFLPLISTAGNSKDAIKGKTLNSTSISELLIPISNYREMKKI
                     VSKVDLLFQKVAQLSD"
     gene            complement(1732..3285)
                     /locus_tag="ACUBV3_001247"
     CDS             complement(1732..3285)
                     /locus_tag="ACUBV3_001247"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HHU3847751.1"
                     /translation="MTPEQLKASILQRAMEGKLVPQDPTDEPASELLKRIKAEKENLI
                     ADGKIKRDKKETELFRGADGKPYEKLADGTIQEVEVPYEIPESWNWVKLRNIGSITSG
                     GTPKSSEPSYYGGNITWITPADMGKQQNNKFFAKSSKKITELGLQKSSAQLISKNSIV
                     YSSRAPIGHINIVTEDYTTNQGCKSITPLLVDLIFLYWLLQFRTKDIILRSSGTTFKE
                     ISASGFGDTLLPLPPLAEQKRIVAQIEKALAKVDEYAESYNKLQQLDKEFPDKLKKSI
                     LQYAMQGKLVAQSPDDEPVEVLLEKIKAEKQKLYEEGKLKKKDLEELVVTKGDDNSPY
                     GKIPKNWSFLTIKDIFSITTGLSYKKTDLAITKNGVRIIRGGNINPLSFKILDNDYYI
                     DPKFITSETVYLKRNQLLTPVSTSLEHIGKFARIDKDYPNTAAGGFVFQLTPFVSSDV
                     LSKYLLFSLSSPIFYEQLKSITKLSGQALYNIPKTKLNELLVPLAPETEQKRISQRVE
                     QLFEKVNQL"
     gene            complement(3285..4748)
                     /locus_tag="ACUBV3_001248"
     CDS             complement(3285..4748)
                     /locus_tag="ACUBV3_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HHU3847752.1"
                     /translation="MSITSFVKRIQDITRNDAGVNGDAQRIEQMSWLLFLKIYDSREL
                     VWELEEDDYESIIPEDLKWRNWAHAEKGEQVLTGDDLLDFVNNRLFKELKELEITPNM
                     PIRKSIVKSAFEDANNYMKNGVLLRQVINVIDEVDFNSPEDRHSFNDIYEKILKDIQS
                     AGNSGEFYTPRAATDFIAEMLDPKLGETMADLACGTGGFLTSTLNYLSKQRKTSEDIQ
                     KYNQAVFGIEKKAFPHLLAVTNLFLHEIDDPKIIHGNTLEKNVREYTDDEKFDLIMMN
                     PPFGGSELDTIKNNFPAELRSSETADLFMAVIMYRLKENGRVGVILPDGFLFGEGVKT
                     RLKEKLVEEFNLHTIIRLPHSVFAPYTGIHTNILFFDKTKKTEQTWFYRLDMPEGYKN
                     FSKTKPMKSEHFNPVREWWTSREEILEGNFYKAKSFTPSELADLNYNFDQCGFPKEEE
                     EILEPLELIQNYQQERAALNQKIDAVLADILELLEEN"
     gene            complement(4761..7085)
                     /gene="hsdR"
                     /locus_tag="ACUBV3_001249"
     CDS             complement(4761..7085)
                     /gene="hsdR"
                     /locus_tag="ACUBV3_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EcoAI/FtnUII family type I restriction enzme
                     subunit R"
                     /protein_id="HHU3847753.1"
                     /translation="MATFIKDKKSMSEEDIKANFITPAILAKGWKNGEHIAYEEYFTD
                     GRIEVRGDKDRRKEGKKSDYALYYQFGQRIAIVEAKDNKHSLRAGLQQAIEYGEILDV
                     PFVYSSNGNGFIEHDRITRQERELTLEEFPTREELFERLISEKEISSEVSKAISVPYY
                     TDAFSNKTPRYYQQIAINRTVEAVARGENRVMFVMATGTGKTFMAFQIIHRLRKAGLA
                     KRVLFLADRNILVDQTMAEDFKPFEKVMTKITAKLLNSPEKLNSFEIYLGLYQQLTGE
                     DGEETHYQKFDKDFFDLIIIDEAHRGSAKEDSNWRQVIDYFASATQIGMTATPKETKE
                     VSNIDYFGEPIYTYSLKQGIEDGFLAPYRVMRVNLNIDVDGYRPENGKTDAAGKLIED
                     RYYGRKDFDKTIVIDDRTQKVAKFVSDYMKQNNARFDKTIVFCVDIDHAERMRAAFVK
                     ENPDLVQEDYRYVMQVTGDNAEGKAQLDNFMDVNSKFPAIVTTSKLLTTGVNAKTCRL
                     IVLDSNIQSMTEFKQIIGRGTRLYPQKGKEFFTIIDFRNVTNLFADPDFDGEPAYVFD
                     PKGPTGGGGESTGGGEPIEKYRVTDKKVDVLNSTIQVLDENGKLVTESLTDYTRKNIL
                     GAYATLTEFITAWQAADKKRVILEELYKKGVYLDAIREAEGISEQEIDDFDLLLKLAY
                     GQKELTKSQRISRVKSSGYLQKYSEEARAVLETLLDKYMDKGIGELESIETLKLPEFQ
                     IYGGTFKIINTYFGNKQAYLRAIKELEEELFTVA"
     gene            complement(7429..7785)
                     /locus_tag="ACUBV3_001250"
     CDS             complement(7429..7785)
                     /locus_tag="ACUBV3_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HHU3847754.1"
                     /translation="MKNRIKNIVKQVFLTEDENKRLLALMANEKESNFSAFARKKLFN
                     QEIESWTINFPEYEQLTERFIQIGRSINRIAKLSTQVGNISQQNFIELGQLITQLLDE
                     LEKGLSVNLKKKSTKH"
     gene            complement(7782..8147)
                     /locus_tag="ACUBV3_001251"
     CDS             complement(7782..8147)
                     /locus_tag="ACUBV3_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein"
                     /protein_id="HHU3847755.1"
                     /translation="MVERYRTVLKKFYITKSQNQTLNYLISYTGLRNFSNYARKMLFK
                     KFPIVLVFDETSFEDLIFSLRRIKNNINQLARIAEQSQDLQALRAMTYSVQMIEKYEK
                     SFLKYHKTKKARLLSKVDE"
     gene            complement(8140..8430)
                     /locus_tag="ACUBV3_001252"
     CDS             complement(8140..8430)
                     /locus_tag="ACUBV3_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5962 family protein"
                     /protein_id="HHU3847756.1"
                     /translation="MTIYYQLENMLVAGFKSEEELQRYQGLKAEYEEETLDYSFSIRE
                     IVNQLEIIIQSRENDFPNLEEGLLQDYQELVEYLREYDVSQAEKYTRRIYCG"
     gene            complement(8441..9295)
                     /locus_tag="ACUBV3_001253"
     CDS             complement(8441..9295)
                     /locus_tag="ACUBV3_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HHU3847757.1"
                     /translation="MSEVQWIKITTDIFDDEKIQLIESMPEGDTLIVIWFKLLVLAGK
                     QNYSGVLALGDKVFYTEEMLTTVFRRKSTTVKLALKTFEQFGMIAFVDGAVTIPKWEK
                     HQNIDGLEKIRKQTRERVARHRQKQKALVSGQDSLSIECNVTETLPVTESNGQVTQQI
                     KNKNQKENKNVLDNSSRETEDQSTTYLKPEYYFLLQVIADRYNDRFIYPYNYTLTHKQ
                     KMAIGQFLSEGYMTSDEVLGMIDRIPNDVESPLAYLMSSMERLKDERLLEAKVIAHEN
                     ARKFYQKG"
     gene            complement(9307..9591)
                     /locus_tag="ACUBV3_001254"
     CDS             complement(9307..9591)
                     /locus_tag="ACUBV3_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HHU3847758.1"
                     /translation="MSEEATLVVQLPKVLEKQLRAHYDEMVSTAIERALEDKELYKPM
                     VRMAALSRWLDVSTTTLQKWTREGMPTMVIDGVTLYDKRAVTRWLKQYER"
     gene            9738..10256
                     /locus_tag="ACUBV3_001255"
     CDS             9738..10256
                     /locus_tag="ACUBV3_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HHU3847759.1"
                     /translation="MTNHIAKLIEESGKKLTEISSATNIAYPTLSGYNQGIRQPKKGN
                     AQKLADYFGVSMAYILGLDDNKQAPSTMNIISDSFKISEISSVTPFKSDMEKLKKGIE
                     LGEIHLSMPLNDGFSDDFRRILARYLVNNEEEFMKTFTKHMNSLKRESEIWQTWIQTE
                     EYRIRQEDRHKK"
     gene            10311..11465
                     /locus_tag="ACUBV3_001256"
     CDS             10311..11465
                     /locus_tag="ACUBV3_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HHU3847760.1"
                     /translation="MSVHKYQSTKGITYFVKVYLGLNDYGKKKYYTKRGFKTRKAAKA
                     HETAVNHQLNSGTFVHITAQTTYTYQELYQRWYEAYKDTVEATTAVKTADLYRLHILP
                     VFGEKKISIISPLDCQTFITDKAKTFKNMKQIKSYTSKIFEFAINMNYIERNPMSKVI
                     MPKLKKTVSENYWTVNELHHFLTIILENEPYKHYALFRLLAYSGLRKGELYALKWEDF
                     DSENQLLTVSKSLGRIDGHAIEKGTKNTFSVRSIYLDDETCSILNKWKQETSREKWQL
                     SVQPLSLDKEFMFTYCNRDGEIEPLHADYINNILKRIIRKHNLKKISPHGFRHTHATL
                     MIEMGIDPVNTAKRLGHASSQITLDTYSHATKAGEKQSITKFAEYLNKAK"
     gene            complement(11611..12003)
                     /gene="rpsI"
                     /locus_tag="ACUBV3_001257"
     CDS             complement(11611..12003)
                     /gene="rpsI"
                     /locus_tag="ACUBV3_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HHU3847761.1"
                     /translation="MAQAQYAGTGRRKNAVARVRLVPGTGKITINKKDVEEYIPHADL
                     RLVINQPFAVTSTQGSYDVFVNVVGGGYAGQSGAIRHGISRALLEVDPDFRDSLKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(12024..12470)
                     /gene="rplM"
                     /locus_tag="ACUBV3_001258"
     CDS             complement(12024..12470)
                     /gene="rplM"
                     /locus_tag="ACUBV3_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HHU3847762.1"
                     /translation="MNKTTFMAKPGQVERKWYVVDATDVPLGRLSAVVASVLRGKNKP
                     TFTPHTDTGDFVIVINAEKVKLTGKKASDKIYYTHSMYPGGLKQISAGELRSKNAVRL
                     IEKSVKGMLPHNTLGRAQGMKLKVFVGGEHTHAAQQPEVLDISGLI"
     gene            12771..>12846
                     /locus_tag="ACUBV3_001259"
     CDS             12771..>12846
                     /locus_tag="ACUBV3_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000160532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HHU3847763.1"
                     /translation="MTKHKHLTLSDRNDIQSGLDRGETF"
BASE COUNT         3899 a         2569 c         2155 g         4223 t
ORIGIN      
        1 tatatgctaa attacggccc aatctaaaga aagttatcat ttctgacgaa aacggtttcg
       61 cgacaacaga acttcttccg attaaagttt ttgggaatat ttcgctagat tacataagat
      121 actgtatgat ttcaccgttt tattatttta atattatcca gtcggtttat ggtgttaaaa
      181 tgccaagggt aagttctggt tttctaaatt caactctcct ccccctcccg cctctcaccg
      241 aacaacagcg tattgtttct aaaatttctc aagtcttttc agttatcgaa tctttagtat
      301 aatacttatg tgcggtggaa tttctatcaa aaatcagata ggagttgaag atgaaagact
      361 ttgaattaca tttacgaaaa gctggattag ctgaaaacac catacggtct tacctgtatg
      421 gtgttcgctt ttttttggaa cattatgagt taaaaataga agatttattt gaatataaag
      481 gatatttact ggataatttc aaacctaaaa ccgttaatct gcgattgcag gggcttaata
      541 aataccttgt ttttattgga cacgatgatt tgaaattaaa atttgttaaa atccagcaga
      601 aaccattttt agaagatgtt atcagccatg cagattatct ctttctgaaa cggagtttga
      661 aaaaagatga taacttaaaa tggcattttg tagtttggtt tctaggagca acaggagctc
      721 gtgttagtga attgataaaa ttaaaagtag aacatgtgga ggtcggttat tttgatattt
      781 actcaaaggg gggtaaaatg cgacgactct acatccctaa aaaattacgt gacagttgtc
      841 agaaatggct tgatactgaa aatcgccaaa gcgggtacct tttccttaat aaatttagtc
      901 agccgattac agctagaggg atcgcccaac aactaaaaaa ctatgctgta aaatatcaac
      961 tcaataccaa ggtcgtctat cctcattcct ttagacacct atttgccaaa aattttcttg
     1021 ccaaatacaa tgacattgcc ctactagcag atttgatggg acatgaaagc atcgaaacaa
     1081 ctcgtatcta ccttagaaaa acagcgacag agcaacaagc tattatagat agggttgtta
     1141 attggtaaca taaatagcag aaagtctttg tgactttctg ctgtttttgt gacggttgct
     1201 ctccttatag gaatagcaaa aaaaatagtg attttgtcgg ttcaactatg gctgagattc
     1261 ctaactcttg gagctacgtt aagtttggtt cgattgttac ctttaacatt ggaaaaacac
     1321 cacccaggaa cgaaccgact tattggggca atgatatccc atgggtatct atttcggata
     1381 tgccgagcag tggtcatatt actaaaacaa aggagtgtat atctcattta gctataaagc
     1441 aaacaaatat taaaattgtt cccgcagata ccctactgat gagttttaaa ctatcaatcg
     1501 gcaaggttgc gatattagat gttccagcca gtcataatga ggcaattatt tctatatttc
     1561 catatagtga taagaaaaat attattagaa attatttaat gatgttttta ccactaattt
     1621 ctacggctgg aaattcaaaa gatgctataa aaggcaaaac actaaatagc accagtattt
     1681 cagagttatt gatacctatt tcaaattatc gggaaatgaa aaaaattgta tctaaagttg
     1741 atttactttt tcaaaaagtt gctcaactct ctgactaatg cgtttttgct ctgtttcagg
     1801 agctagggga acaaggagtt cgtttaattt tgttttagga atattatata aagcctgtcc
     1861 agatagcttt gtaattgatt tcaattgttc ataaaagatt ggtgatgaca ggctaaataa
     1921 tagatatttt gacaatacat ctgatgaaac aaaaggtgtt aattgaaata caaatccacc
     1981 agcagcggta ttgggataat ctttatcaat tctagcaaat tttccaatat gttctaaaga
     2041 tgttgaaaca ggtgttaata gctgatttcg tttcaaatag actgtttctg aagtgataaa
     2101 ttttggatca atgtagtaat cattatctaa tattttaaag gataatgggt tgatatttcc
     2161 tccacgaata attctgacac cattttttgt gatggctaga tcagttttct tatacgataa
     2221 tcctgtggtt attgaaaaaa tatcttttat ggtaagaaaa gaccaatttt tgggtatctt
     2281 cccataagga gagttatcat ctccttttgt taccacaagc tcttccaaat ctttcttctt
     2341 gagtttacct tcctcataga gtttttgctt ttcagctttg attttttcaa gcaagacttc
     2401 aacaggttca tcatctggac tttgagctac tagcttacct tgcatggcgt attggaggat
     2461 agatttcttc agtttatctg gaaattcttt gtctagttgt tgtagtttat tgtaactttc
     2521 tgcatactcg tctacttttg ctaatgcttt ttcgatttga gcgacaatcc gtttttgctc
     2581 agcgagggga gggaggggga gaagtgtatc tccaaaacct gatgctgata tttccttaaa
     2641 tgttgtccct gaagaacgta aaataatatc tttggttcta aactgtagta accaatataa
     2701 aaaaattaaa tcaacaagta gaggtgttat tgatttacaa ccttgatttg tagtgtagtc
     2761 ttcagtaaca atatttatat gtccgattgg agctcgacta gaataaacaa tagagttttt
     2821 tgaaattagt tgagcagaag atttttgtaa acctaattca gtaatttttt tcgatgattt
     2881 agcaaaaaac ttattatttt gctgttttcc catatctgct ggagttatcc aagtgatatt
     2941 cccaccatag taggaaggtt cagaagattt tggcgtgcct ccagaggtaa tactaccaat
     3001 atttcttaat ttcacccagt tccaactctc tggtatctca tatggtactt caacttcttg
     3061 gatagttcca tctgccaact tctcataagg tttcccgtca gcacctcgaa aaagctctgt
     3121 ttctttttta tctcgcttga ttttgccatc agcaatgagg ttttcttttt ccgctttgat
     3181 acgtttaaga agctcactag ctggctcgtc agttgggtct tgaggaacca atttcccctc
     3241 catcgctctc tgtaagatac tagcttttag ttgttctggg gtcattagtt ctcctccaaa
     3301 agctctaaaa tatctgccaa aacagcatct attttttggt tcaaagccgc tctttcttgc
     3361 tgatagtttt gaataagttc cagcggttcc aaaatctctt cttcttcctt gggaaagcca
     3421 cattgatcaa aattatagtt caaatcagct aactcagatg gtgtaaaaga tttggcttta
     3481 taaaaatttc cctctaaaat ctcctcacgg ctagtccacc actcacgaac aggattgaaa
     3541 tgctctgatt tcatcggttt ggtcttagaa aagtttttat acccctctgg catatccaaa
     3601 cgataaaacc aagtctgctc cgtcttctta gtcttgtcaa agaaaaggat attggtgtga
     3661 atacctgtgt aaggtgcaaa gacactatga ggcaaacgga taatcgtatg aagattaaat
     3721 tcctcaacca atttttcttt caaacgagtt ttaacaccct ctccaaagag aaagccatct
     3781 ggtaaaatta ccccaacacg accgttttct ttcagcctat acatgataac tgccataaaa
     3841 aggtccgctg tctccgaact ccgcaactct gccggaaaat tatttttaat cgtatcaagt
     3901 tcggaaccac caaaaggtgg gttcatcatg ataaggtcaa acttctcgtc atctgtgtac
     3961 tctcggacat ttttctccaa ggtattgcca tgaatgattt tagggtcatc aatttcatga
     4021 agaaagaggt tcgtcactgc taaaaggtga ggaaaggctt ttttctcaat cccaaagaca
     4081 gcctgattgt atttttgaat atcctcgcta gtcttacgct gtttgcttaa ataatttaaa
     4141 gtcgaggtca agaaaccacc tgttccacag gcaagatcag ccattgtttc acctaattta
     4201 gggtcaagca tttcagcaat gaaatcggtc gctgcacgag gggtataaaa ttccccagag
     4261 ttccctgcac tttggatgtc cttgaggatt ttctcataaa tatcattaaa ggagtgtcgg
     4321 tcttctggac tattaaagtc cacctcatca atgacattga taacctgacg caagagcaca
     4381 ccgtttttca tgtagttatt agcatcttca aaggctgatt taacaatgct tttccgaatg
     4441 ggcatattgg gtgtgatttc aagctctttt aattccttaa acagcctatt gttcacaaaa
     4501 tcaagcaaat catcacctgt caagacttgc tcaccttttt cagcgtgagc ccaatttcgc
     4561 cattttaagt cttcaggaat aattgactca taatcatctt cctccaactc ccaaaccaac
     4621 tcacggctat cataaatttt caaaaagagt aaccaagaca tttgttcaat acgttgggcg
     4681 tctccattaa cacctgcgtc attgcgtgta atatcttgaa tacgtttcac aaatgatgta
     4741 attgacatag tttcctttca ttaagctact gtaaatagct cttcttccaa ttctttaatg
     4801 gcccttagat aggcttgctt atttccaaaa taggtattga taatcttaaa ggttccaccg
     4861 taaatttgaa actctggtaa ttttagcgtt tcaatgcttt ccagttcacc aatcccctta
     4921 tccatgtact tatctaataa cgtctctaaa acagctcggg cctcttcact gtacttttgc
     4981 agataaccac tacttttaac cctgctaatt ctctggctct tagtcaactc tttttgacca
     5041 taagccagtt ttaacagcaa gtcaaagtca tcaatctcct gttcagaaat cccttctgct
     5101 tctcgaatag catcaagata aacgcctttt ttatagagtt cttctaagat gactcttttc
     5161 ttatccgctg cttgccaggc agtaataaac tcggtcaagg tagcgtaagc tcctagaata
     5221 tttttccgag tataatcggt cagactttct gtcactaact tcccattttc atccaaaacc
     5281 tgaatggttg aattgagaac atctactttt ttatcagtga ccctatattt ttcaataggt
     5341 tctcctcctc cagttgattc gccgccacct ccagttggcc ctttagggtc aaagacatac
     5401 gctggctctc catcaaaatc tggatctgca aataaattgg tcacatttct aaaatcaata
     5461 atggtgaaaa attctttccc tttctgcggg tacaaacgtg ttccacgacc aataatctgt
     5521 ttgaactccg tcatggattg aatattggaa tcgagcacaa tcaaacgaca agtttttgca
     5581 ttgacccccg ttgtcaagag ttttgatgtt gtgacaatag ctggaaactt agaattaaca
     5641 tccataaagt tatctagctg tgctttaccc tcggcattgt cgccagtcac ctgcatcaca
     5701 tagcgatagt cttcctgaac caaatcaggg ttttctttga caaaagctgc tcgcatccgc
     5761 tcagcatggt caatatcaac acaaaagaca atggttttgt caaatcgagc attgttctgt
     5821 ttcatgtaat cagaaacaaa cttggccacc ttctgagtac ggtcatcaat aacaatagtc
     5881 ttatcaaaat cttttcgacc atagtaccta tcttcgataa gttttcctgc ggcatccgtt
     5941 ttcccatttt ctggacgata gccatcaaca tcaatattca aattgacccg catcacgcga
     6001 taaggagcca ggaagccatc ctcaatgccc tgtttcaaac tataggtgta aattggttca
     6061 ccaaaataat ctatattgga aacctctttt gtctcctttg gtgtcgctgt catcccaatc
     6121 tgggttgctg aggcaaaata atcaatcact tggcgccagt tgctgtcctc cttagctgaa
     6181 ccgcggtgag cctcatcaat aataatcagg tcaaagaaat ccttatcaaa cttttggtaa
     6241 tgcgtttctt cgccgtcttc acctgttaat tgctgataca aacctaagta aatttcaaaa
     6301 gaatttaact tttctggaga gttcagtaac tttgctgtga ttttggtcat aaccttttca
     6361 aaaggcttaa aatcttcagc cattgtttgg tcaactaaga tatttcggtc ggctaaaaat
     6421 agaacacgtt tggctaaacc agcttttcgc aaacgatgga taatttgaaa agccataaag
     6481 gtcttacctg tccctgttgc cataacaaac atgacccgat tttctcctct tgcaaccgcc
     6541 tcaacggtac ggttgatagc gatttgctgg taataacgtg gtgttttatt ggagaacgca
     6601 tcagtatagt aagggactga aatcgccttt gaaacctcag aacttatctc tttttcagaa
     6661 atcaaacgct caaataattc ttcacgagta ggaaactcct ctaaagttaa ctcacgttct
     6721 tgcctcgtga tacggtcatg ctcaataaaa ccattaccat ttgaggaata aacaaaagga
     6781 acatctaaaa tctcaccata ttcaatcgcc tgctgtaatc cagctcgtaa actatgctta
     6841 ttatctttag cttcaacgat tgcaatacgc tgcccaaatt gatagtagag agcatagtct
     6901 gattttttcc cttctttacg acgatcctta tcacctcgaa cctctattcg cccatctgta
     6961 aaatattcct cataggcaat atgctcacca tttttccaac ctttagctaa aatggctggt
     7021 gtgatgaaat tcgctttaat atcttcttcg ctcatgctct ttttgtcttt gatgaatgtc
     7081 gccataccaa gtctctccac atctacactt aataaaaaat tatcagaagt atgttacccg
     7141 tctattatac cattttctaa cctcatcttg tattatttct caaggggttt ggggctttgc
     7201 cccaagtgtt aaaatccatc aggagaagga gctcccaaac tcgttactga tggtttattt
     7261 tttcgatagt gtgtatacac tatctgagct cgcaaagacc taaaattatg tttttgtatc
     7321 aattctaaat tccccgtgcc agacacggtg gctgacattg tcagacaatc attttttaga
     7381 aggacataag aaaaccgcag ataaattcct gcggtttcat ttacttattt aatgtttagt
     7441 agacttcttt ttcaggttaa ctgagagtcc cttttccaat tcatccaata attgagttat
     7501 caactgacca agttctataa aattctgttg tgaaatattt ccaacctgag ttgacaactt
     7561 ggcaatccta ttaattgatc tgccaatctg aatgaatcgc tctgtcagtt gctcatactc
     7621 tggaaaatta atcgtccatg attcaatctc ttggttaaat aatttctttc tggcaaaagc
     7681 ggaaaagttt gactcttttt cattagccat taatgctaac aatcttttat tctcatcctc
     7741 agtcaaaaag acttgcttca cgatattttt aattctattt ttcattcatc cacttttgat
     7801 agtaacctcg cctttttcgt tttatggtat ttaaggaacg atttttcata tttttcaatc
     7861 atttgaacac tgtaagtcat agctcgtaat gcttgaagat cctgagattg ctcagctatc
     7921 cttgctaact gattgatatt atttttaatt cgcctcaaag aaaatatcaa gtcttcaaat
     7981 gaagtttcat caaataccaa aacaataggg aatttcttaa ataacatttt cctagcatag
     8041 ttagaaaaat tccttaaacc tgtataactg ataaggtagt ttagcgtttg attctgcgat
     8101 tttgtgatat agaatttttt cagaactgtt cgataacgct caaccacaat aaattctcct
     8161 tgtgtatttt tctgcctgag acacatcgta ttccctcaaa tattctacaa gttcctgata
     8221 atcttgcaac agcccttctt ctaaatttgg gaagtcattt tctctactct ggataataat
     8281 ttctaattga ttgacaattt ctctaattga gaaactataa tccagggttt cttcctcata
     8341 ctctgctttt aacccttgat accgttgtaa ctcttcttct gatttaaaac cagcgactag
     8401 catattctcc aattgataat aaattgtcat tatactctcc ttatcccttt tggtaaaact
     8461 ttctagcatt ttcatgagca attaccttag cttccaataa tcgctcatct tttaggcgct
     8521 ccatagagct cattaaatag gctaatggtg actctacatc attaggaata cgatctatca
     8581 ttcccaagac ttcatcagaa gtcatatagc cttcacttaa aaactgtcca atcgccattt
     8641 tttgcttatg cgttagggtg taattatagg ggtaaataaa acgatcgtta tatctatccg
     8701 caatcacttg caaaaggaaa taatactctg gtttgagata agttgttgac tgatcttctg
     8761 tctctctaga actattatct agtacattct tattctcttt ctgattcttg ttcttaatct
     8821 gttgcgttac ttgaccgtta ctttccgtta caggtaacgt ttctgtaacg ttacattcta
     8881 ttgataaact atcttgccca cttactagag ctttttgctt ttgacgatga cgagccactc
     8941 tttctcttgt ttgtttacga attttttcta gaccatcaat attctgatgt ttctcccatt
     9001 taggaattgt gactgctcca tccacaaaag caatcattcc aaattgttca aatgttttca
     9061 gagccagctt cacagtcgtt gactttctcc taaagactgt agttaacatc tcttctgtat
     9121 aaaagacctt atctcctaga gctaagactc cactgtaatt ctgtttacct gctaaaacga
     9181 gtagtttaaa ccagatgaca attaaagtat ctccttcggg cattgactca atcaactgta
     9241 ttttttcatc atcaaagata tctgtagtaa tcttaatcca ttgtacttca ctcatacgtg
     9301 tacctcctat ctttcatatt gctttagcca tctagtgaca gctcgtttat cgtacagagt
     9361 caccccatca atgaccattg taggcattcc ttctcgtgtc catttttgaa gggttgtcgt
     9421 tgatacatca agccacctag aaagagctgc catccgaacc ataggtttgt atagttcttt
     9481 atcttctagc gctctttcaa tagctgtaga gaccatttca tcataatgtg cccttaactg
     9541 tttttccaaa actttgggaa gttgaacaac taaagtcgct tcttcagaca tattattacc
     9601 tcgtttccat ttctcaatta aataaactcc gaatttgttt gttattcatt ttgaattata
     9661 tattctttta gaatttttgt caactatttt tattcaaaaa gaattaaatg tgttataata
     9721 ctctcaagga ggtatttatg accaatcata ttgcaaaact gatagaagag agcggcaaaa
     9781 aactgactga gattagttct gctacaaata tcgcttatcc aaccttatca ggatacaatc
     9841 aaggcattcg tcaaccgaaa aaaggaaatg ctcaaaaatt agctgactat ttcggcgtat
     9901 ccatggctta catcttagga cttgatgata acaaacaagc accatcaact atgaacatta
     9961 tttcggacag ttttaaaatt tccgagatta gttctgtaac accttttaaa agcgatatgg
    10021 aaaagctgaa gaaaggaatt gaactaggag aaatccatct gtctatgccc ttaaatgacg
    10081 gcttctcaga cgattttagg cgcattttag ctcgctattt ggttaataat gaagaagaat
    10141 tcatgaaaac cttcactaaa cacatgaata gtttaaaacg agaatcagaa atttggcaaa
    10201 cctggattca gaccgaagaa taccgaatta gacaagaaga tcgacataaa aaataaacta
    10261 acagtttctc aattacataa gctccgaaaa cttagaaacg gagaactgtt atgtctgtac
    10321 acaagtacca atctacaaaa ggaatcacct attttgttaa agtttacctt ggtctaaacg
    10381 actacggtaa gaaaaaatat tatactaaac gagggttcaa gacgcgtaaa gctgccaaag
    10441 ctcatgaaac tgctgttaat catcagttga atagtggaac ttttgtccat attactgctc
    10501 aaacaaccta cacttaccaa gagctttatc aaaggtggta tgaagcttat aaagatactg
    10561 ttgaagcaac tactgcagtc aaaactgccg acctctatcg cctacatatt cttcctgttt
    10621 ttggagaaaa gaaaatttca ataatcagtc ctttagactg tcaaaccttc atcactgaca
    10681 aagccaagac attcaaaaac atgaagcaaa tcaaatctta tacttctaaa atatttgaat
    10741 ttgccattaa tatgaattat attgaacgaa atcccatgag caaggttatt atgcctaaac
    10801 tcaaaaaaac tgtcagtgag aactattgga cagttaacga gttgcatcat tttttgacaa
    10861 tcattttaga aaatgaacct tataaacatt atgcactatt tcgattacta gcatacagcg
    10921 ggttacgaaa aggagagctc tacgccctta aatgggaaga ttttgattct gagaatcaac
    10981 tattaacagt tagcaaaagt ttaggaagaa tagatggcca tgccattgaa aaaggaacta
    11041 aaaatacatt ctctgtgcgc tctatctatc ttgatgatga aacctgttct atcctcaaca
    11101 aatggaaaca ggaaactagc agagaaaagt ggcaattatc agtacaacct ctttctctcg
    11161 acaaagaatt tatgtttaca tactgcaacc gagatggtga gatagaaccc ttacacgctg
    11221 actatatcaa caatattcta aaacgtatta tacgtaagca taatcttaaa aagattagcc
    11281 cacatgggtt tagacacact cacgcaacct tgatgattga aatgggaatt gacccagtca
    11341 atactgctaa acgcttaggc catgcaagca gccagataac tttagacact tatagccatg
    11401 ctacaaaagc tggtgaaaaa cagtcaatta caaaatttgc agagtatctt aataaggcta
    11461 aatgaactag atttattgct cagcctcata ttgaccaaaa accaagtctc gaaagattac
    11521 caaaaggatt accaaagcac ttttttcatc aaaaaagcac cttgcgaaaa cttcgcaaag
    11581 tgctctgaaa cgttgatata atcgtattga ttaacgtttt gagaattgtg atgccttgcg
    11641 ggcttttttg agacctggtt ttttacgttc aaccatacgt gcgtcacgtg taagaagtcc
    11701 agcacgtttc aatgaatcgc ggaaatctgg gtcaacttct aaaagtgcac gtgagatacc
    11761 atgacggatc gcacctgatt gacctgcata tccaccacct acaacgttaa caaaaacatc
    11821 gtatgaacct tgagttgaag taactgcgaa aggttggttg ataacaagac gaaggtctgc
    11881 gtgtgggatg tactcttcta catctttttt attgattgta attttaccag tacctggaac
    11941 caaacgaacg cgtgcaacag cgtttttacg gcgaccagta cctgcatatt gtgcttgtgc
    12001 cattatataa tgctcctttc ctcttagata agtcctgaga tatcaagtac ttctggttgt
    12061 tgtgcagcat gtgtatgctc accgccaaca aatactttca atttcatacc ttgtgcacgt
    12121 ccaagagtgt tatgtggaag catgccttta actgattttt caatcaaacg aacagcattt
    12181 ttagaacgaa gttcacctgc tgagatttgt ttcaaaccac ctggatacat tgagtgagtg
    12241 tagtagattt tatctgatgc ttttttacca gttaatttaa ctttttcagc attgataaca
    12301 atcacaaagt cacctgtatc agtgtgtggt gtgaaagttg gtttgttttt tccgcgaagt
    12361 acgctagcaa ctactgcaga aagacgtcca agaggtacat ctgttgcgtc aacaacgtac
    12421 catttgcgtt caacttggcc tggtttagcc atgaaagttg ttttgttcat gatttctcct
    12481 atacgaattc gtttttgttt acaggtgtgg atgttccggt ccacgagagt atttgaaagg
    12541 ttccggggcc ttacaaatgg ggtaaacaat accgcttata atcatatcaa aataaggtag
    12601 cattcgtcaa gttatttctt ttattttatc aattttcatc ccaaaataaa tcatcagtaa
    12661 tttctcaaag taagttccgt ctgccctcca aaaccggaag ttagtgtgaa aaggattttt
    12721 atggtggaat taagtctgag acgtttgggt tagaaatgag tttatccatc atgacaaaac
    12781 acaaacacct aactctctca gaccgtaatg acatccaatc tggtttggat agaggagaaa
    12841 ccttta
//
LOCUS       DBJYSH010000059        12718 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.059, whole genome shotgun sequence.
ACCESSION   DBJYSH010000059 DBJYSH010000000
VERSION     DBJYSH010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 12718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12718
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.059"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            complement(<1..1264)
                     /locus_tag="ACUBV3_001260"
     CDS             complement(<1..1264)
                     /locus_tag="ACUBV3_001260"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0015936 - coenzyme A metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HHU3847764.1"
                     /translation="MTKISWTGFSKKSPEERIHYLEEQDFLADSSLEIVTNQDLLSLS
                     LANQMAENVIGRIALPFSLVPDVLVNGKVYQVPYVTEEPSVVAAASFAAKIIKRSGGF
                     LTTVHNRKMIGQVALYDVQDSQHTKESILNQKQQLLEIANAAHPSIVKRGGGACDLTI
                     EIKEDFLIVYLMVDTKEAMGANMVNTMMEALSSPLEDISKGKSLMSILSNYATESLVT
                     ATCRVDLRFLSRQKEEAIKLAQKMTMASQLAQVDPYRASTHNKGIFNGIDAIVLATGN
                     DWRAIEAGAHTYAVKDGQYRGLSRWSYKVDDNCLEGTLTLPMPVATKGGSIGINPSVH
                     LAHDLLGRPNAKELASIILSIGLAQNFAALKALVSTGIQAGHMKLQAKSLALLAGAKE
                     EQISEVVKQLLDSKHMNLETAQKIVNKLT"
     gene            complement(1266..2438)
                     /locus_tag="ACUBV3_001261"
     CDS             complement(1266..2438)
                     /locus_tag="ACUBV3_001261"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001923259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HHU3847765.1"
                     /translation="MKIGIDKIGFATSQYVLEMTDLAIARQVDPEKFSKGLLLDSLSI
                     TPVTEDIVTLAASAANDILSDEDKETIDMVIVATESSIDQSKAASVYVHQLLEIQPFA
                     RSFEMKEACYSATAALDYAKLHVEKHPDSKVLVIASDIAKYGIKSTGESTQGAGSIAM
                     LISQNPSILELKEDHLAQTRDIMDFWRPNYSDVPYVNGMFSTKQYLDMLKTTWKVYQK
                     RFNTSLSDYAAFCFHIPFPKLALKGFNKILDNNLDEQKKAELQENFEHSITYSKKIGN
                     CYTGSLYLGLLSLLENSQNLKAGDQIAFFSYGSGAVAEIFTGQLVDGYQNKLQSDRMD
                     QLNKRQKITVTEYEKLFFEKTILDENGNANFNTYRTGTFSLDSICEHQRIYKKINN"
     gene            2592..3431
                     /locus_tag="ACUBV3_001262"
     CDS             2592..3431
                     /locus_tag="ACUBV3_001262"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HHU3847766.1"
                     /translation="MTKADLLFKDNITKIMSEGVFSEQARPRYKNGEMANSKYITGAF
                     AEYDLSKGEFPITTLRPIPIKSAIKEIFWIYQDQTNDLAVLNDKYGVTYWNDWEVGHT
                     GTIGQRYGAVVKKHNIISKLLKQLEDNPWNRRNVISLWDYEAFEETEGLLPCAFQTMF
                     DVRRVNGELYLDATLTQRSNDMLVAHHINAMQYVALQMMIAKHFGWRVGKFFYFINNL
                     HIYDNQFEQAQELLKRQPSECNPKLVLNVPDGTDFFDIKPDDFALVDYDPIKPQLRFD
                     LAI"
     gene            3511..4005
                     /locus_tag="ACUBV3_001263"
     CDS             3511..4005
                     /locus_tag="ACUBV3_001263"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HHU3847767.1"
                     /translation="MTKQIIAIWAEDEDHLIGVNGGLPWRLPKELHHFKETTMGQALL
                     MGRKTFDGMNRRVLPGRETIILTKDEQFQADGVTVLNSVEQVIKWFQEHNKTLFIVGG
                     ASIYKAFLPYCEAIIKTKVHGKFKGDTYFPDVNLSEFKVISRDYFEKDEQNAHAFTVT
                     YFEK"
     gene            4223..5449
                     /gene="clpX"
                     /locus_tag="ACUBV3_001264"
     CDS             4223..5449
                     /gene="clpX"
                     /locus_tag="ACUBV3_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HHU3847768.1"
                     /translation="MAGNRNNDMNVYCSFCGKSQDEVKKIIAGNGVFICNECVALSQE
                     IIKEELAEEVLADLAEVPKPKELLEILNQYVVGQDRAKRALAVAVYNHYKRVSYTESS
                     DDDVDLQKSNILMIGPTGSGKTFLAQTLAKSLNVPFAIADATSLTEAGYVGEDVENIL
                     LKLIQAADYNVERAERGIIYVDEIDKIAKKGENVSITRDVSGEGVQQALLKIIEGTVA
                     SVPPQGGRKHPNQEMIQINTKNILFIVGGAFDGIEDLVKQRLGEKVIGFGQTSRKIDD
                     NASYMQEIISEDIQKFGLIPEFIGRLPVVAALELLTAEDLVRILTEPRNALVKQYQTL
                     LSYDGVELEFDQDALLAIADKAIERKTGARGLRSIIEETMLDIMFEIPSQEDVTKVRI
                     TKAAVEGTDKPVLETA"
     gene            5460..6056
                     /gene="yihA"
                     /locus_tag="ACUBV3_001265"
     CDS             5460..6056
                     /gene="yihA"
                     /locus_tag="ACUBV3_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HHU3847769.1"
                     /translation="MAEEFLNTHNASILLSAANKSHYPQDDLPEVALAGRSNVGKSSF
                     INTLLGRKNLARTSSKPGKTQLLNFYNIDDKLRFVDVPGYGYAKVSKTERAKWGKMIE
                     EYLVTRDNLRVVVSLVDFRHDPSADDIQMYEFLKYYEIPVIIVATKADKIPRGKWNKH
                     ESSIKKKLNFDKKDHFIVFSSVDRTGLDESWDTILSEL"
     gene            complement(6118..6666)
                     /locus_tag="ACUBV3_001266"
     CDS             complement(6118..6666)
                     /locus_tag="ACUBV3_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HHU3847770.1"
                     /translation="MTESVTVNKKRIKQITQTQEDILTAFGQLLEEYPYEKIQISQIA
                     KKSGHARRTFYRHFDSRDDLLTLFIERLTLNLFKQLGQLEQPTFSQVFQNFFSYWSDY
                     KSLLLILRKNDLLPQFQQSWFRHIDLIELGRGDLSSNTYAQRFAIGGIFSVLIEWIHQ
                     DCQTSIEELTQLSFDIINHLKN"
     gene            6758..7261
                     /locus_tag="ACUBV3_001267"
     CDS             6758..7261
                     /locus_tag="ACUBV3_001267"
                     /inference="COORDINATES: protein motif:HMM:NF025160.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HXXEE domain-containing protein"
                     /protein_id="HHU3847771.1"
                     /translation="MKALITKWYLFCPYLASLFALALFFGNWDLRVQSLLISGLFIQL
                     HFFEEFGFPGGFPLITMLVELKSVETDTSKWDLNHLSAFFGNQWFAVIVYLLPIFCPN
                     IPFLTLAVMIFAFAELAMHLFFFNLSLKKWYNPGLLTTLVGLVPVSVYYLAHDWKLYS
                     GLDWFLG"
     gene            7419..8069
                     /locus_tag="ACUBV3_001268"
     CDS             7419..8069
                     /locus_tag="ACUBV3_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HXXEE domain-containing protein"
                     /protein_id="HHU3847772.1"
                     /translation="MSYFRNYWYRFGAILFIILAVILLVFRPDWSMLHYLLYFYFMAL
                     LAHQFEEYQFPGGASPIINYVVYDEEELMDCFPGNTQSIMLVNTIAWLLYIASIAFPQ
                     AYWLGLGVMFFSLTQLLGHGFQMNIKLKTWYNPGLATTVFLLVPIACAYIYQASAEGM
                     LTWGDWLGGFIMLIVCVLTSIIAPVQLLKDKETNYIISPWQMDRFHKVVNFVRIKK"
     gene            8199..9575
                     /locus_tag="ACUBV3_001269"
     CDS             8199..9575
                     /locus_tag="ACUBV3_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HHU3847773.1"
                     /translation="MENHRYEDEGKFQRKMTSRHLFMLSLGGVIGTGLFLSSGYTIAQ
                     AGPLGAVLSYLIGAVVVYLVMLSLGELAVAMPVTGSFHTYATKFISPGTGFTVAWLYW
                     ICWTVALGTEFLGAAMLMQRWFPNVPAWAFASFFALVIFGLNALSVRFFAEAESFFSS
                     IKVIAIIIFIILGLGAMFGLVSFEGQHKAILFTHLTANGAFPNGIVAVVSVMLAVNYA
                     FSGTELIGIAAGETDNPKEAVPRAIKTTIGRLVVFFVLTIVVLASLLPMKEAGVSTAP
                     FVDVFDKMGIPFTADIMNFVILTAILSAGNSGLYASSRMLWSLANEGMLSKSVVKINK
                     HGVPMRALLLSMAGAVLSLFSSIYAADTVYLALVSIAGFAVVVVWLAIPVAQINFRKE
                     FLKENQLEDLSYKTPFTPVLPYITIILLLISIVGIAWDSSQRAGLYFGVPFIIFCYIY
                     HKLRYKKW"
     gene            9580..10524
                     /gene="mmuM"
                     /locus_tag="ACUBV3_001270"
     CDS             9580..10524
                     /gene="mmuM"
                     /locus_tag="ACUBV3_001270"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000532339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047150 - betaine-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase"
                     /protein_id="HHU3847774.1"
                     /translation="MGRFKELLESKKTLILHGALGTELESRGCDVSGKLWSAKYLIED
                     PAAIQTIHEDYIRAGADIVTTSTYQATLQGLAQVGVSESQAEDLIRLTVQLAKAAREQ
                     VWKSLTKEEKSERIYPLISGDVGPYAAFLADGSEYTGLYDIDKQGLKNFHRHRIELLL
                     DEGVDILALETIPNAQEAEALIELLAEDFPQVEAYMSFTSQDGKTISDGSAVADLAKA
                     IDVSPQVVALGINCSSPSLVADFLQAIAEQTNKPLVTYPNSGEVYDGASQSWQSSPDH
                     SHTLLENTSDWQKLGAQVVGGCCRTRPADIADLSAHLK"
     gene            complement(10617..>12718)
                     /locus_tag="ACUBV3_001271"
     CDS             complement(10617..>12718)
                     /locus_tag="ACUBV3_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HHU3847775.1"
                     /translation="NNFYNRDPFGNMDDIFNSLMGNMGGYNSENKRYLINGREVTPEE
                     FSQYRQTGKLPGQELNNQNTPTNQVSADSVLTKLGTNLTDQARQHLLDPVIGRNKEIQ
                     ETAEILARRTKNNPVLVGDAGVGKTAVIEGLAQAIINGDVPAAIKNKEIISIDISSLE
                     AGTQYRGSFEENIQNIIKEVKETGNIILFFDEIHQILGAGSTGGDSGSKGLADILKPA
                     LSRGELTVIGATTQDEYRNTILKNAALARRFNEVKVNAPSAQDTFNILMGIRNLYEQH
                     HNVVLPDSVLKAAVDLSIQYIPQRSLPDKAIDLIDMTAAHLAAQHPVTDLKSLEKEIA
                     EQRDKQEKAVNTEDFEEALKVKTRIEELQNQIDNHTEGQKVTATINDIAMSIERLTGV
                     PVSNMGASDIERLKELGNRLKGKVIGQNDAVEAVARAIRRNRAGFDDGNRPIGSFLFV
                     GPTGVGKTELAKQLAFDMFGSKDAIVRLDMSEYNDRTAVSKLIGATAGYVGYDDNSNT
                     LTERIRRNPYSIVLLDEIEKADPQVITLLLQVLDDGRLTDGQGNTINFKNTVIIATSN
                     AGFGNEAFTGDSDKDLKIMERISPYFRPEFLNRFNGVIEFSHLSKDDLSEIVDLMLDE
                     VNQTIGKKGIDLVVDENVKSHLIELGYDEAMGVRPLRRVIEQEIRDRITDYYLDHTDV
                     KHLKANLQDGQIVISER"
BASE COUNT         3663 a         2275 c         2545 g         4235 t
ORIGIN      
        1 tagtaagttt attgacaatt ttttgtgctg tctctagatt catgtgttta gagtctagaa
       61 gctgtttaac gacttctgaa atttgttctt ctttagctcc agcaagtagt gccaacgact
      121 ttgcttgaag tttcatgtga ccagcttgaa ttcctgtgct aaccaatgcc ttaagagccg
      181 caaaattttg agctagtcca atcgaaagaa taatacttgc caactcttta gcattaggac
      241 gacctagtag atcgtgagct aggtggacac tagggttaat accaatggat ccaccttttg
      301 ttgcaacagg catcggtagc gtcagagtac cttctaggca attatcatca accttataag
      361 accatctaga taatcctcga tattgcccat ctttgacagc ataggtatgc gctccagctt
      421 caatagctcg ccaatcattt ccagtagcta agacgatagc atcaatgcca ttaaaaatac
      481 ctttattatg tgtgctagca cgataagggt ctacttgagc taattgactt gccatcgtca
      541 ttttttgagc taacttgatg gcctcctctt tttggcgact tagaaatctt aaatcaactc
      601 ggcatgtagc agtcactaaa gattcggtag catagttaga taaaattgac attaagcttt
      661 tgccttttga aatgtcttct aatggcgaag ataatgcttc catcatggtg ttaaccatat
      721 tagcacccat ggcttctttt gtatcaacca taaggtaaac aatcaaaaag tcttctttaa
      781 tctcaatagt taggtcgcaa gcaccgcctc cacgcttgac tattgatgga tgagcagcgt
      841 tggctatttc taataattgc tgtttttgat ttaatataga ttctttagtg tgttgactat
      901 cttgtacatc ataaagggct acttgtccaa tcattttacg attatggacg gttgtcagaa
      961 aaccaccaga tcgttttata attttagcag caaaacttgc agcagcaaca actgacggtt
     1021 cttcagtgac atatgggact tgatagactt taccgttaac caaaacatct gggacaagtg
     1081 aaaatggtaa ggctattcga ccgatgacat tttcagccat ttgattggct agtgataaag
     1141 agagtaagtc ctgattcgtc actatttcta gggaagaatc agctaaaaaa tcttgttctt
     1201 caaggtaatg aatacgttct tcaggggatt tttttgaaaa tcctgtccag gaaatttttg
     1261 tcatattaat tattaatttt cttgtagata cgttgatgtt cgcaaataga gtctagagaa
     1321 aaagtgccag tccggtaggt gttgaagtta gcatttccat tttcgtctag gattgttttt
     1381 tcaaagaaga gtttctcata ctcagttact gtaatttttt gacgtttatt gagttgatcc
     1441 atacgatcgc tctgaagttt attttggtag ccgtctacta actgaccggt aaaaatttca
     1501 gcaacagctc cgctaccgta tgaaaagaag gcaatttgat cacctgcttt taaattttga
     1561 ctattttcta gtaacgatag aagtcctaga tacaatgagc cagtatagca atttccaatt
     1621 ttcttactat aggtaataga gtgctcaaaa ttttcttgta gttcagcttt cttttgttca
     1681 tctaagttat tatctagaat tttattaaag ccctttaaag ctaattttgg aaaaggaata
     1741 tggaaacaaa aagcagcata gtcacttaga ctagtattaa aacgtttttg atatactttc
     1801 caagtagttt ttagcatatc tagatattgt tttgttgaaa acatgccatt aacataagga
     1861 acatcagaat aatttggtcg ccaaaagtcc ataatatcac gggtttgcgc tagatggtcc
     1921 tcttttagct ctagaatgga tggattttga ctaataagca tagcgatact gcctgcacct
     1981 tgagtggatt ctccagtaga tttaatgcca tatttagcaa tatctgaagc tatcactaat
     2041 actttagaat ctggatgttt ttctacatgt aatttagcgt aatcaagagc agcagttgcg
     2101 ctatagcatg cttctttcat ctcgaatgac cttgcgaaag gttgaatctc taaaagttga
     2161 tgtacataga cagaagccgc cttactctga tcgatacttg attctgtagc gacaatcacc
     2221 atatcaatag tttctttgtc ctcatcagat aaaatatcat tagctgcaga agctgcaagt
     2281 gtaacgatat cttctgtaac tggcgtaata ctaagtgaat ctaacaataa tcccttgctg
     2341 aatttttcag ggtcaacttg gcgagcaata gccaaatcag tcatttcaag gacatattga
     2401 ctggttgcaa aaccaatctt atcaattcct attttcattg gtaaaccttt ctgaataaaa
     2461 gtcatttttg ctaaattcca ttttaccata aagaagctaa aaatatgaaa aaaagcttta
     2521 accttcaaag tcttgtttct cactagaata tctttttaaa atctgataaa ataagacttt
     2581 ggaggtatct catgacaaaa gccgatttat tatttaaaga caatatcacg aaaattatgt
     2641 ctgaaggtgt atttagcgag caagccagac ctagatataa aaatggggaa atggctaatt
     2701 ctaagtatat tacaggtgcc tttgcagagt atgatttatc aaagggtgaa tttcctatta
     2761 caactctacg tccaattcca ataaaatctg ctattaaaga aattttctgg atttatcaag
     2821 atcagacaaa tgatttagca gttttgaatg ataaatatgg tgtgacatac tggaatgatt
     2881 gggaagttgg ccatactggc acgataggtc agcgctacgg tgcagttgtt aaaaaacata
     2941 atattatatc aaaactctta aaacagcttg aagataaccc ttggaatcgt cgtaatgtta
     3001 tttcattatg ggactatgaa gcatttgaag aaacagaagg cctactacct tgtgcttttc
     3061 aaacgatgtt tgatgtgaga cgcgttaatg gtgagcttta tttggatgcc acattaacac
     3121 aacgttctaa tgacatgtta gttgcacatc atattaatgc tatgcagtat gtagcacttc
     3181 agatgatgat tgcaaagcat tttggttgga gagttgggaa gtttttctat tttatcaata
     3241 atcttcatat ttatgataac cagtttgaac aagctcagga actcttaaaa cgccaaccgt
     3301 ctgagtgtaa ccccaaacta gtattgaatg ttccagatgg aacagacttt tttgatatta
     3361 aaccagatga ttttgcgtta gttgattatg acccaattaa acctcaatta cgttttgatt
     3421 tagcaatcta actatctcat gttttttaac aagcaatttg aggtaaaatt tgttagaata
     3481 tctagagata agttagtgag gaaaaaagtc atgacaaagc aaataattgc catttgggct
     3541 gaagatgaag accatttgat tggagttaat ggcggtttac catggaggct tcctaaagag
     3601 ttacatcact tcaaagaaac gaccatgggg caggctttgc ttatgggacg aaagaccttt
     3661 gatggaatga accgtcgtgt tttacctggt agagagacaa tcatcttaac aaaagatgaa
     3721 caattccaag cagatggagt gacagtccta aatagtgttg aacaagttat aaaatggttt
     3781 caggaacata ataagacctt atttattgta ggtggtgcaa gtatttataa agcatttctg
     3841 ccttattgtg aagcaatcat aaaaactaaa gttcatggaa aatttaaagg tgatacctat
     3901 tttcctgatg ttaatctatc tgagtttaaa gttatctcta gagattattt tgaaaaggat
     3961 gagcagaatg ctcatgcttt tactgtgaca tattttgaaa aataaggaag gaatactatt
     4021 caatgcaacg tagccttttt ggggtcttta ccgcattttt agctgtgatt tgtgttttat
     4081 gtgcaattcc tgctataaag aaaaaaagat atggattagg aacagttttt ttgatgaatg
     4141 ctttcactaa ccttgtcaac acaatccatg ccttttatgg aacattattt taaattaaat
     4201 gaatagaaaa gttgaggaaa aaatggctgg gaatcgtaat aacgatatga atgtctattg
     4261 ttcattttgt ggcaaaagcc aagatgaagt aaaaaaaatt attgcaggta atggtgtttt
     4321 catttgtaat gaatgtgtgg ccttatcaca agaaattatt aaggaagaat tagctgagga
     4381 agtactggct gatttagcag aagtaccaaa acctaaggaa ctattagaaa tattaaatca
     4441 atatgttgta gggcaagatc gtgctaaacg tgctttagca gttgctgtct acaatcatta
     4501 caagcgtgtt agttataccg agagtagtga cgatgatgta gatttgcaaa aatccaacat
     4561 tttgatgatt ggtccaactg gctcaggaaa aaccttctta gcacaaacac tggctaaaag
     4621 ccttaatgta ccgtttgcta ttgcagatgc gacttcattg accgaagcag gatacgttgg
     4681 agaagatgtt gagaatattc ttcttaaatt gattcaagct gctgattata atgtcgaacg
     4741 tgctgagcgt ggtattatct acgttgatga aatagataaa attgctaaga aaggcgaaaa
     4801 tgtttctatc acacgtgatg tgtctggtga aggtgtacag caagcccttc ttaaaattat
     4861 tgagggtacg gtagcaagtg ttcccccaca gggtgggcgt aaacatccta accaagaaat
     4921 gattcaaatt aataccaaga acatcctttt tattgtcggt ggtgcttttg atggtattga
     4981 agaccttgtg aagcaacgtt taggcgaaaa agttattggt tttggacaga caagccgtaa
     5041 aattgatgac aacgcttctt atatgcaaga gataatttct gaggatattc aaaagtttgg
     5101 actgattcca gagtttattg gccgtttacc agtagttgca gcgttagaac ttcttactgc
     5161 agaagatctg gttcgtattc tgacagaacc acgcaatgct ttggttaaac aataccaaac
     5221 cttattatct tatgatggtg tagaattgga atttgaccag gatgctctat tggctatcgc
     5281 tgataaggct atcgagcgca agactggtgc acgtggttta cgttctatta ttgaagaaac
     5341 gatgcttgat atcatgtttg aaattccaag ccaagaagat gtaacaaaag ttcgtatcac
     5401 aaaggctgct gttgagggta ctgacaagcc tgttttagag acggcttagg gagatagaga
     5461 tggcagaaga atttttaaat actcataatg cgtctatttt attgagtgca gctaacaagt
     5521 cacattatcc tcaggatgat ctcccagaag ttgctctcgc tggtcgttca aatgttggta
     5581 aatcaagctt tatcaacacg cttcttggtc gtaaaaacct agcgcgtacg tcgagtaagc
     5641 caggtaagac acaactgctc aacttttata acattgatga caaactgcgt tttgtcgatg
     5701 tccctggata tggctacgct aaggtgtcta aaactgagcg tgctaagtgg ggcaagatga
     5761 ttgaagagta ccttgtaaca cgtgataacc ttcgtgttgt tgttagttta gttgattttc
     5821 gtcatgatcc aagtgcggac gatattcaaa tgtatgaatt tcttaagtac tatgagattc
     5881 cagtaattat tgtagcgaca aaggcagata aaatacctcg tggcaagtgg aataaacatg
     5941 agtcgtctat taagaagaaa ttaaactttg ataaaaagga ccatttcatc gtcttttcat
     6001 ctgttgaccg cacaggtctt gatgaatcat gggatactat tttatctgag ttataatttg
     6061 agagtttatg acaaaaagag ctttgcagta gctgttgaga ctactgcaaa gctcttttta
     6121 attttttaaa tgattgataa tatcgaagga aagttgagtt aattcttcga tacttgtttg
     6181 gcagtcctga tgaatccact caattagaac actgaaaata ccaccaatcg caaatcgctg
     6241 agcataggta ttggaagata aatcaccacg acctaactct atcaaatcaa tatgtcggaa
     6301 ccaggattgt tgaaattgag ggagtaggtc attctttcta agaatgagaa gaagggattt
     6361 ataatcagac caataactaa aaaaattttg gaaaacttgg ctaaaagtag gttgctccaa
     6421 ttgaccaagt tgcttaaaga gatttaatgt tagtctttcg atgaaaaggg taagtaggtc
     6481 atctcgactg tcaaagtgtc ggtaaaaggt ccttctagca tggccagatt tcttggcaat
     6541 ttggctaatc tgaattttct catagggata ctcttccaaa agttggccaa aagctgttaa
     6601 gatatcctct tgtgtttgag tgatttgttt tattctcttt ttattaactg tcacactttc
     6661 agtcatttgt gcataaaacc tccaaaggta ttgatattat aggttcactt gattatacta
     6721 tatggtatca aatgtcacaa atcttggagg tcatcttatg aaagcattga taacaaaatg
     6781 gtacctcttt tgtccctatc ttgcttctct ttttgcctta gcattatttt ttggaaattg
     6841 ggatttacga gtgcagtcac tacttatttc gggacttttt attcaattac actttttcga
     6901 agaatttggt ttccctggag gttttccgct tatcactatg ctagttgaac ttaaatcggt
     6961 tgaaactgat actagtaagt gggacttaaa ccatctatca gcctttttcg ggaatcagtg
     7021 gtttgcagtg attgtttatc ttttgccgat tttttgtccc aatataccat ttttaacact
     7081 tgcagtcatg atttttgcat ttgcagaact tgccatgcat cttttctttt ttaatctttc
     7141 cttgaagaag tggtataatc caggacttct tacgacctta gtaggtcttg tccctgtttc
     7201 tgtctattat ttagctcatg attggaagtt gtattctgga cttgattggt ttttaggctg
     7261 atatggattg ttcttaacta ctttatagcc tttcgttccc caatctataa gcgtttaggt
     7321 cgacattcaa attacgcatt tagtgatgtt gatctttcac gctcaaaacc atttttaaca
     7381 cattttcgtg aaacacagtt taaataggag gtattatcat gtcatatttt agaaattact
     7441 ggtatcgttt tggagcaatt ttatttatta ttttagcagt aatattgctt gtttttagac
     7501 ctgactggtc aatgcttcac tatctattgt atttttactt tatggcactt ctagcgcatc
     7561 aatttgaaga atatcagttt cccggtgggg catcacctat cattaactat gttgtttatg
     7621 atgaagaaga gctgatggat tgttttccag gcaatactca gtctattatg ttggttaata
     7681 ctattgcttg gttgctttac attgctagta ttgcttttcc tcaagcttat tggcttggat
     7741 taggagtcat gttctttagt ctaacgcagc tcttgggtca tggttttcag atgaatatta
     7801 aacttaaaac ttggtataat cctggtctag caacgacagt atttctccta gtaccaatag
     7861 cttgcgcata catctatcaa gctagtgcag aaggaatgct cacttgggga gattggctag
     7921 gtggttttat catgttgatt gtctgtgtac taactagcat tattgcacct gtacagctat
     7981 tgaaggataa ggagaccaat tatattatta gtccttggca aatggaccgt tttcataagg
     8041 tcgttaattt tgtaaggata aaaaaataag gttaccgcta tttatgattc gctgattttt
     8101 tgtaatagtt tttctttatc atatatgata gctaatacct attccccaat acgactagat
     8161 tgtggtagga taaaagtatt agtttcttgg aggttgttat ggaaaatcat cgttatgaag
     8221 atgaaggtaa attccagcgt aagatgacca gtcgtcatct ctttatgtta tcgctaggtg
     8281 gtgttatcgg gactgggctt ttcttgagtt caggttatac cattgcacag gctggtccgc
     8341 ttggagctgt gctgtcttat ttgattggtg ccgttgtggt ttatttggtc atgctatcac
     8401 ttggggaatt ggcggttgcc atgccggtga cggggtcatt ccacacttat gccactaagt
     8461 ttatcagtcc tggaacaggt tttactgttg cttggctata ttggatttgt tggacggtcg
     8521 ccttggggac tgaattttta ggtgctgcca tgctgatgca gcgctggttc ccaaatgtgc
     8581 cggcttgggc atttgcttcc ttttttgccc ttgtgatttt tggtttaaat gctcttagcg
     8641 tacgcttttt tgcagaagca gagtctttct tctcaagtat taaggttatt gctatcatta
     8701 tctttattat cttgggctta ggtgctatgt ttggtctagt ttcctttgaa ggtcagcaca
     8761 aggctattct cttcactcat ctgactgcca atggtgcctt tccaaatggt atcgttgcag
     8821 ttgtctcagt catgttggct gttaactatg ccttctctgg tactgagtta attggtattg
     8881 cggctggtga aacggataat cccaaagaag ctgtaccaag ggctattaaa acgacaatcg
     8941 gtcgcttggt tgttttcttt gtactgacaa ttgttgtcct agcttcgcta ttgccaatga
     9001 aagaggcagg cgtatccaca gcaccattcg ttgatgtctt tgacaagatg ggaatccctt
     9061 ttacggcgga tatcatgaac ttcgttatct tgacagccat cctgtctgct ggtaactcag
     9121 gtctctacgc atcaagccgt atgctctggt cccttgccaa tgaaggtatg ttgtcaaaat
     9181 ctgttgtgaa aatcaataaa cacggtgtcc caatgcgtgc tcttctcttg tcaatggcag
     9241 gagcagtgct gtcgctcttt tcaagtattt acgctgcaga cacagtttat ctagccttgg
     9301 tttcaatcgc gggctttgct gttgttgtcg tatggctagc cattccagtc gcacaaatca
     9361 atttccgcaa ggaattcttg aaagagaatc aactagaaga tttgtcttac aaaacacctt
     9421 ttacaccagt tctcccttat atcacaatca ttttgctctt gatttcgatt gtagggattg
     9481 cttgggatag ctcacaacgt gctgggcttt acttcggtgt tccatttatt attttctgct
     9541 atatttatca caaactacgt tacaagaaat ggtgatctta tgggaagatt taaagaactt
     9601 ttagagagta aaaaaactct catcctacat ggtgccttgg ggaccgagct tgagagtaga
     9661 ggttgtgatg tttctgggaa actttggtca gccaaatatc tgattgagga tccagcagct
     9721 attcagacca ttcatgaaga ttatattcgt gctggcgctg atattgtgac gacatcaact
     9781 tatcaggcga ctttacaagg actagctcaa gttggtgtct ctgaaagtca ggcagaagat
     9841 ttgattcgct tgactgttca gttggcaaaa gctgcgcgtg agcaagtttg gaaaagtttg
     9901 acaaaagaag aaaaatcaga gagaatctat cctttgattt ctggggatgt tggtccctac
     9961 gctgcctttt tagcggatgg ctctgagtac actggactct acgatattga taagcaaggt
    10021 cttaaaaact tccatcgcca tcgtattgag cttcttttag atgagggtgt tgacattctg
    10081 gcacttgaaa ccattccaaa cgctcaggag gctgaggctc ttattgagct acttgccgag
    10141 gatttccctc aggttgaggc ttatatgtca ttcacctctc aagatggtaa gaccatctca
    10201 gatggtagtg ctgttgcaga cttggcgaaa gctattgatg ttagcccaca ggtcgtggcg
    10261 ctgggcatta actgttcaag tccatcgtta gttgctgatt tcttgcaagc aatagcagag
    10321 cagactaaca agcctcttgt gacctatcca aactctggag aggtttatga tggggcaagc
    10381 caatcttggc aatctagccc tgatcattca cacaccctac ttgaaaatac aagtgactgg
    10441 caaaaacttg gcgcccaagt tgtcggaggt tgttgccgaa ctagaccagc tgatattgct
    10501 gacctctctg cacatttaaa ataagtaaag tatcttgaag aaggttaggg taagtttgat
    10561 ttcctttttc aatccactag aaaacccgct tggattcttc caaacgggtt ttgtttttat
    10621 ctttcagaaa tgacgatttg gccatcttgc aaattagctt ttaggtgttt aacgtctgta
    10681 tgatcgagat agtagtctgt gatgcgatct cgaatttctt gctcgatgac acggcgcaat
    10741 ggacgtactc ccattgcttc gtcataaccc agttcaatta agtgtgattt aacattttca
    10801 tctaccacaa ggtcaattcc ttttttgcca attgtttggt taacttcatc aagcatcaaa
    10861 tctacaattt cgcttaagtc atctttgctt aggtgagaga attcaataac accattgaaa
    10921 cgatttagaa attctggacg gaaatatgga gaaattcgtt ccataatttt caagtcttta
    10981 tcgctgtcac ctgtaaatgc ttcgtttcca aaacctgcat ttgaggttgc aatgataaca
    11041 gtatttttaa agttaatagt gtttccttga ccatcagtta aacggccatc atctagaact
    11101 tgaaggagaa gcgtgataac ttgcgggtca gctttttcga tttcatcaag aagaacaata
    11161 gaataaggat ttcgacggat acgttctgtt agtgtattac tattgtcgtc ataaccaacg
    11221 taaccagcag tcgctccaat tagcttagaa actgctgtac gatcattata ttcagacata
    11281 tcaagtctaa cgatagcatc ctttgatccg aacatatcaa aagctaattg ctttgctagc
    11341 tcggttttac caactccagt tggtccaaca aatagaaagc taccaatcgg acgattacca
    11401 tcatcgaaac cagcacgatt tcggcgaata gctctcgcaa ctgcctcaac agcatcattt
    11461 tgaccaatga ctttaccttt tagacgattg ccaagttctt ttaagcgttc aatatcactg
    11521 gcacccatat ttgatacagg aactccagtt agacgctcaa ttgacatagc gatatcgttg
    11581 atagttgcag tgactttttg tccttcggta tgattatcaa tctggttttg taactcttca
    11641 atgcgtgtct tgaccttaag agcttcttca aaatcttcag tattgacagc tttttcttgc
    11701 ttgtctcttt gctcagcgat ttctttttct aaggatttaa ggtcagttac gggatgttgc
    11761 gcagctaggt gtgcagcagt catatcaata aggtcaattg ctttatcagg gagacttctt
    11821 tgtgggatgt attgaataga taaatctaca gctgctttca aaacagagtc aggtaaaaca
    11881 acattatgat gttgctcata gaggtttctt atccccataa ggatattgaa agtatcttgt
    11941 gcagaaggtg cattgacttt aacttcgtta aagcgacgtg ctaaggcagc attcttgaga
    12001 atggtgttac gatattcatc ttgagtagtt gcacctataa ccgttaactc accacgtgaa
    12061 agtgcaggct taagtatatc tgcgagcccc ttagagccac tatcaccacc agttgaaccg
    12121 gctcctaata tttggtgtat ttcatcaaag aagagaataa tatttcctgt ttccttaact
    12181 tcttttatga tattttgaat attttcttca aaactacccc tatattgggt gccagcttca
    12241 aggctagaaa tatctatcga aatgatttct ttatttttga tagcagcagg gacatcacca
    12301 tttataattg cttgtgccaa accttcaata acagctgttt taccaacacc tgcatcacca
    12361 accagaacag gattattttt agtacgacga gctagaattt ctgcagtttc ctgaatttct
    12421 ttgtttcgtc caataactgg atctagcaaa tgctgacgtg cttgatcggt taaattagtt
    12481 cctaatttag tcaaaacact atctgcagag acttggtttg taggagtgtt ttggttattt
    12541 agctcttgac cggggagttt tccagtttga cgatattgac tgaattcttc aggagtaact
    12601 tcccgtccat tgattaagta tcgcttattc tcactgttat agccccccat atttcccatt
    12661 agagaattaa aaatatcatc catatttcca aaagggtcac gattataaaa attattat
//
LOCUS       DBJYSH010000060        12655 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.060, whole genome shotgun sequence.
ACCESSION   DBJYSH010000060 DBJYSH010000000
VERSION     DBJYSH010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 12655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12655
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.060"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            57..1556
                     /locus_tag="ACUBV3_001272"
     CDS             57..1556
                     /locus_tag="ACUBV3_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HHU3847776.1"
                     /translation="MTTKAKHDIHGKPYNRTAMITLLLIATFAGVLNQTSLGTAIPTL
                     MNSFNISLSTAQQATTWFLLANGIMIPVSAYLATRFSTKWLYVTSYVVLLIGLLMTTL
                     APTSNWNLFLVGRIIQAISVGISMPLMQVVMVNVFPPEQRGAAMGLNGLVVGLAPAIG
                     PTLAGWILKQEFHFAGHDLTWRAIFLLPLLILTVTTILSPFVLKDVVDNKSVKLEVPS
                     LILSIIGFGSFLWGFTNVATYGWGDIGYVISPIMVGIIFIALFIHRQLKLETPFLDIR
                     VFKNKQFSVTTAAIALSMMAMMGVEMMLPLYLQNVHGLSALDSGLALLPGALMMGIVS
                     PISGAVYDKVGARRMAMIGFTILGVATLPFVFLTTTTPDHFITLLYAVRMFGIAMVMM
                     PLTASAMSALPPHEAAHGTAANNTARQIASAVVVALLSSVAQNIITNNKPSKDLLTMN
                     PLKYANQMLNASLDGFHVSFAIGFVFAVLGLLVSLFLRKGKIIETEKEV"
     gene            1558..2274
                     /locus_tag="ACUBV3_001273"
     CDS             1558..2274
                     /locus_tag="ACUBV3_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000584725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HHU3847777.1"
                     /translation="MIILIIALATILTFVTWMLIRPKALRYILGLLSLALLFASVWML
                     TDHFVNHTGMTIETKTKTREIYSAGDSNAPFGLMIYKEIGTHSSNNVLVYRTDLNQKE
                     AKAHFVPNTKEVTSAVKKSASYQLADVKKATVTTKTKRYVWKDSLSKLLYGFAGENGE
                     LISQKSVAKIPRKTWLVLTKEQSEHLESAAAKLKEQMTLQAKVNPVKAMEIKTLAKTN
                     PEAYAEMQVKALKEALNIKE"
     gene            2374..3441
                     /locus_tag="ACUBV3_001274"
     CDS             2374..3441
                     /locus_tag="ACUBV3_001274"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000505344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HHU3847778.1"
                     /translation="MGFEQRLTRCQTAISQLSCDALLITNLTNIFYLTGFSGTNATVL
                     ISPKHRIFVTDSRYALIAKNIVREFDIIISREPLAAILKIIRDDALMAIGFETDISYH
                     MYKHMVEVFEDYRLIEAPSVVEKLRMIKDATELAIIRQACRISDQAFLDVLDFIKPDR
                     TTELQVANFLDFRMRELGATGPSFDFIVASGYRSAMPHGVASQKTIQSGEALTLDFGC
                     YYQHYVSDMTRTIHIGHVTDQEREIYDIVLKSNQAIIDNVKSGMKRCDYDYLARQVIE
                     NSGYGNHFTHGIGHGMGLDVHEIPYFGKSEGVIASGMVVTDEPGIYLDNKYGVRIEDD
                     LLITETGCEVLTSAPKELIVL"
     gene            3453..3905
                     /locus_tag="ACUBV3_001275"
     CDS             3453..3905
                     /locus_tag="ACUBV3_001275"
                     /EC_number="3.5.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004132 - dCMP deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006220 - pyrimidine nucleotide metabolic
                     process [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxycytidylate deaminase"
                     /protein_id="HHU3847779.1"
                     /translation="MNRLSWEDYFMANAELISKRSTCDRAFVGAVLVKNNRIIATGYN
                     GGVSETDNCNEVGHYMEDGHCIRTVHAEMNALIQCAKEGISTNNTEIYVTHFPCINCT
                     KALLQAGVKKITYKANYRPHPFAIELMEAKGVAYVQHDVPEVTLGMEE"
     gene            3953..4327
                     /locus_tag="ACUBV3_001276"
     CDS             3953..4327
                     /locus_tag="ACUBV3_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HHU3847780.1"
                     /translation="MHRSFLEENYLEAIYILQQRNESVRSIDVAEYLSVAKSSVNSAV
                     NKLLTQQYITFEDDKIIRMTAEGEKIAEFVYEKHLYLKKLLCKMGIDETEADEIACLL
                     EHILKEDSFKKIKKFVQDKKII"
     gene            4544..5359
                     /locus_tag="ACUBV3_001277"
     CDS             4544..5359
                     /locus_tag="ACUBV3_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HHU3847781.1"
                     /translation="MIKNGDSIAVVNGDIELSYKDIDRQSNSFANGLLKAGFKCGDKI
                     VLQLPNYHEFIIIAFAMFKVGIIPIMSLPAHRKNELKGIIEKSEAVAYIAKDKYLGFS
                     YVDMIRDIKIESDREFKVYILGEGDEFKKFSELNDNNYIYQKTEVDYKNIGLLLLSGG
                     TTRIPKLIPRRHCDYLYVAKKTSIISGMDRGSIYLAALPMAHNFPLCCPGILGTFAVG
                     GKVVVCEVTSPDEILPLIEEEKVTITGFVPAIANICMDYLQYEDYDLSSLKIL"
     gene            5459..5902
                     /locus_tag="ACUBV3_001278"
     CDS             5459..5902
                     /locus_tag="ACUBV3_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding enzyme"
                     /protein_id="HHU3847782.1"
                     /translation="MTTSIDDDEKTVWQTQGKPISEPDEILIVDKARKLKDGNYQIAG
                     RIKEIINRAGEKITPLELEEILLTHDCINSVQVLGVPDKLHGEAIAVFILKSESKRLT
                     LEEVRKFLISNNVADFKLPDAVKYIDSWPLTALGKIDRNKLKENF"
     gene            5916..6071
                     /locus_tag="ACUBV3_001279"
     CDS             5916..6071
                     /locus_tag="ACUBV3_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001867180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3847783.1"
                     /translation="MELNGVKENAKILVKDWLKDIFAEEVISEDENLIEKGLSSIKIM
                     QTIFRYA"
     gene            6086..6517
                     /locus_tag="ACUBV3_001280"
     CDS             6086..6517
                     /locus_tag="ACUBV3_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazolinyl imide reductase"
                     /protein_id="HHU3847784.1"
                     /translation="MTSKNPDNYMFLMHKISLTTNSGSLTLSGTNGPIIWEPCLDKPT
                     DENNRFNLEKNEFSELKIFEITEEVEETYNDMMKLSWVEAISKSVIDFTNNIEAEKVD
                     LREQQYLISAIEAWRALSRELGQSNTIQPYKKTAIKMEDLI"
     gene            6555..7124
                     /locus_tag="ACUBV3_001281"
     CDS             6555..7124
                     /locus_tag="ACUBV3_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HHU3847785.1"
                     /translation="MKDLIMIGVFSALYFLFNMVGGIPFAMNPVLTFYQPMGSAFLSG
                     IIFMFLIAKAPKKGTISILAIIMCILRLATGMHWAMAVGTLITGIVAELIAGSKSYKN
                     KKMNMLSFGVFALGDIGTFLVYFMNPKSWSDAMIKKGTDITYIESMNAAAADWMIYVI
                     VIGTFLVALLSAWIGMKLLKKQFEKAGIV"
     gene            7124..7837
                     /locus_tag="ACUBV3_001282"
     CDS             7124..7837
                     /locus_tag="ACUBV3_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HHU3847786.1"
                     /translation="MRKVSNVQFNPCTKIVLLFLCIIISSLLSSIELELIFVFIIALF
                     CIINKKIKFALNGFLIYLGVYFVSILTVQYGSNTLRSLLMPFLGLVHKLYPVCMFSVL
                     ILSTTKVGEFLSAMEFAGVSKKITIPLAVMLRYIPTVREDWIYIKDSMYLRSISPSFF
                     GFIKNPDLTIECIYSPIIIMASKAADELTIASITRGIESPKKRSSIIEIRFDIQDVLA
                     LLIGVVITVFVIYRRFFCD"
     gene            7830..9209
                     /locus_tag="ACUBV3_001283"
     CDS             7830..9209
                     /locus_tag="ACUBV3_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HHU3847787.1"
                     /translation="MIELKGVNFKYKNTKNSCLDNIDLEIKKGEFVLISGASGSGKTS
                     ITRVINKLIPYYYEGELEGTVKINNDLVSSYQMFELSEIVGSVFQNPRTQFFNVDTNS
                     EIVFGLENQGVSRDILKKRLKETCETLDINKLQNRNIFHLSGGEKQKIAFASVYAMNP
                     DIYLLDEPSSNLDIPTIEVLKRHLSVLKNSGKTVIISEHRIYYLMDLVDKVIYMKNGQ
                     IQKSYSKEDFLNLSEDEIKAMALRCRKKTKLNIEMSNNEKHPYLQVNDFSIYRKDNCI
                     INNMNFSANKGDIVAVIGSNGVGKTTFLRTLCGLHDNYKGDVLLNSKKTDAKKRRQKN
                     YMVMQDVNYQLFAESVFAECKLGIENVSDDLIEEILNEFDLSDYKNNHPNTLSGGQKQ
                     RLAIAVGLICKKEIFVLDEPTSGLDYTNMVKTVELLKRYSKDKIIFIATHDTEFANLI
                     CNRVLDLEK"
     gene            9230..10111
                     /locus_tag="ACUBV3_001284"
     CDS             9230..10111
                     /locus_tag="ACUBV3_001284"
                     /inference="COORDINATES: protein motif:HMM:NF012869.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HHU3847788.1"
                     /translation="MDVLRRLYKFAPEKKVYSYASLVLSAIATIFSVVPYFYLWKFLN
                     ELLVLKNLESANKYALWIFMFLVLQTLVYFLSLYCSHMFAFRVERNLKIEGLNNLLEA
                     SFSFFDTNPSGKTRQIIDDNASETHSILAHMQPDMVNAMVYPVILLVVSFLVSISFGI
                     VMVVSLVLGVFLITKMFGNQNFLQKYMASNEDMSSEIVEYIRGVKVIKIFNIGIEKFR
                     SLHKTIITSSELGYAYAISCRKWKVFYDSFFMGLCMVTIPYGVYLLNINTHLGEIISY
                     TVFSQHLLNCYTTLLIK"
     gene            10111..10986
                     /locus_tag="ACUBV3_001285"
     CDS             10111..10986
                     /locus_tag="ACUBV3_001285"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HHU3847789.1"
                     /translation="MFLGQNKAKAKNSIDKLEEIFNKMQVKKLDSGLSDEIQNFDIEF
                     EKVSFKYEGNTPYVLKDLSFKLDSGKSYALIGSSGSGKSTIAKLISGFYDVDSGVIKI
                     GGRDIKEYSRETIASSIAFVFQNAKLFKMSIYDNVRIGNPDATYEEIMQALEKARCNE
                     ILDKFELRENTMIGAEGVYLSGGETQRIVIARAILKNSPIIILDEASAAADPENEYEL
                     QMAFTQLIKNKTVIMIAHRLSAIKNMDEIIVVENGQIIERGSSKLLLEDKSSKYSYFM
                     NMYIRANDWIMSNEV"
     gene            10976..>12655
                     /locus_tag="ACUBV3_001286"
     CDS             10976..>12655
                     /locus_tag="ACUBV3_001286"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HHU3847790.1"
                     /translation="MKSKLMDKYGITEDSSIDLIKASIASFWKNVVYMFPITLILFFL
                     MNISNSKYLQEWFYFVSILIVFVVMYYFTNRQYIKSYSTTFKGSKNLRIELVEIIKRL
                     PLSYFSTHDLTDLSQTVMADVSKIEQALSHAICAYYGWIGYFVFLSLSLLIGSLQLGL
                     CIIVPVITALLILYLSKNIQYRINRKYYKKLRENSQAFQDSFEMQQDIKSYNMQHTVR
                     KDVQDKLKDSEKIQIKTELFTTIPSSLLSILPQFSIALVIIFGFQLYLTGQIQLIFYI
                     GYIICATKVASGFTGMLDSLLMIINFQSSFKQVTAIRKTPLQEGVNADIQTFNVEFRD
                     VSFAYEKGRNVVSNLSFNANQGEVTAIVGPSGCGKSTILKLLSRLYDYNAGQILIGGK
                     DISEISTNSLFENISTVFQDVELFDDTIFENIKCGNPKATIEEVLEAAKIANVDEIAD
                     KFVDKYDTKIGENGSKLSGGERQRISIARAILKDAPIILLDEISASLDIENEIKIQAG
                     LDKLIKNKTVIIVSHRMKSIENANKIIVIENGKVENFGTHSYLLENSTRYRK"
BASE COUNT         4438 a         1736 c         2467 g         4014 t
ORIGIN      
        1 tatttgttat tcttaaaaga tattagttaa aatcagaaat agagattgga gataaaatga
       61 ctacaaaagc aaaacatgat attcatggca aaccatataa tcgtacagcc atgatcacct
      121 tattgttgat tgctacattt gcaggggtgt taaatcagac aagtttggga acggctatcc
      181 caactttgat gaatagcttt aacatttcgt tgtcaacggc tcaacaagca acaacatggt
      241 ttttattagc gaatgggatt atgattcctg tttcagctta tttagcgaca cgtttttcaa
      301 caaaatggtt gtatgtgaca tcctacgttg ttttacttat tggactgtta atgacgacgt
      361 tagcacctac atctaactgg aacctttttt tagtaggacg tattattcaa gcgatttctg
      421 tagggatttc tatgcctttg atgcaggttg ttatggtgaa tgtttttcct ccagagcagc
      481 gtggtgccgc tatgggactt aatggtttgg tggttggtct tgccccagct attgggccta
      541 cattagcagg gtggatttta aaacaagaat ttcattttgc agggcatgat ttgacatggc
      601 gtgcaatttt ccttcttcct ttactaattt taacggtcac tacaatttta tccccctttg
      661 ttctaaaaga tgtggttgat aataagtcag ttaaattgga agtgccttcc cttattcttt
      721 caataattgg ttttggtagt tttttgtggg gcttcacaaa tgtggcaact tatggatggg
      781 gagatattgg atatgttatt tctcctatta tggttggtat tatttttatc gccttattca
      841 ttcatcgtca attaaaacta gaaacaccgt tcttagatat ccgtgttttc aaaaataaac
      901 aattctcagt aacaacagca gctattgcac tttcaatgat ggcgatgatg ggtgttgaaa
      961 tgatgctacc gctttacttg caaaatgttc atggtctctc tgcacttgat tctggtttag
     1021 ctttattacc aggtgccttg atgatgggga tagttagtcc aatttctgga gctgtatatg
     1081 ataaagttgg tgcaagacgt atggctatga ttggttttac catactaggt gtagcgacgt
     1141 taccttttgt tttcttaacg actacaacac cagatcattt tattaccctc ttatatgcag
     1201 tacgtatgtt tggtattgct atggttatga tgccattgac agcaagtgcg atgagcgcac
     1261 ttccaccaca tgaggcagca catggaacag cagctaataa tactgctcgt caaattgctt
     1321 cagcagttgt agttgcattg ctttcaagtg ttgctcaaaa tattatcacc aacaataaac
     1381 catcaaaaga tttgctaaca atgaatcctt taaaatatgc aaatcagatg ttgaatgcta
     1441 gtcttgatgg cttccatgtt tcttttgcca ttggttttgt atttgcggtt cttggtttac
     1501 tggtatctct tttcctgaga aaagggaaaa ttattgagac ggaaaaggag gtatagcatg
     1561 attattttga ttattgcact ggcaactatt ttgacatttg tcacatggat gttgattcgt
     1621 ccaaaagctc tacggtatat tctaggtctt ctctcattag ctttgttatt tgcctcagtt
     1681 tggatgttaa cggatcactt tgttaatcat acaggtatga ctattgaaac taagacaaag
     1741 acacgtgaga tttatagtgc gggtgatagt aacgctccat ttggtctcat gatttataaa
     1801 gagattggta ctcattcgag taataatgtt ttagtttatc gaacagattt aaatcaaaaa
     1861 gaagcaaaag ctcactttgt tccaaataca aaagaagtga caagtgctgt gaaaaaatct
     1921 gctagctacc aattagctga tgtgaaaaaa gccacagtaa caacaaagac taaacgttat
     1981 gtgtggaaag atagtctttc gaaactactt tatggctttg caggtgaaaa tggagaattg
     2041 atttctcaga aatcagttgc taaaatacca cgaaaaactt ggctagtttt gactaaagag
     2101 cagtccgaac acttagaatc agctgcggct aaactaaaag aacagatgac tttgcaagca
     2161 aaagtaaacc cagtgaaagc tatggagata aagacattag ctaagacaaa tccagaagct
     2221 tatgctgaga tgcaagttaa agcattaaaa gaagctctta atataaaaga atagtaaagt
     2281 aaaagcaact gaaatgagtt gcttttctta tagctaaatg tcttaaaaga tgataaaatt
     2341 aatctaatta aggttagaat agcgaggttt cttatgggtt ttgaacaacg tctaactaga
     2401 tgtcaaacag caatttcaca attatcatgt gacgcactct taataacgaa tttaacaaat
     2461 attttttatt tgactggttt ttcaggaact aatgcaacgg tactcattag tccaaaacac
     2521 cggatttttg ttacagattc acgatatgcc ttgatagcaa agaatattgt aagagagttt
     2581 gatattatca ttagcagaga accacttgcg gctattttga aaattattcg agatgacgcc
     2641 ttgatggcga ttggttttga aactgatatc tcttatcata tgtacaaaca tatggtggaa
     2701 gtttttgaag attatcgctt gatagaagct ccatctgttg ttgaaaagtt acgtatgatt
     2761 aaagatgcaa cagaacttgc gatcatccgt caagcctgtc gtatttcgga ccaagctttt
     2821 ttagatgtgt tggattttat aaagcctgat cgaacaactg aattgcaagt tgctaatttt
     2881 ttagactttc gcatgagaga attaggagca actggtcctt catttgattt tattgtagca
     2941 tctggatatc gatcagcgat gccacatggg gttgcgagtc agaagactat tcaatcggga
     3001 gaagctctga ctttagattt tggttgttac tatcagcact atgttagtga tatgacacgt
     3061 acgattcata tcggtcatgt cactgatcag gaacgcgaga tttacgatat cgttttgaaa
     3121 agtaatcaag ctattattga taatgtaaaa tcaggaatga agcgttgtga ttatgattac
     3181 ttggcgcgac aagtcattga aaattctgga tacggtaatc attttaccca cggtataggg
     3241 catggtatgg ggcttgatgt tcatgaaatt ccttattttg gtaagtcaga gggagtgatt
     3301 gcatctggga tggtagtgac tgacgagcca ggaatatatc tcgataataa atacggtgtt
     3361 cgtattgaag atgatttatt aataacggaa acaggttgcg aagtattgac atctgcacct
     3421 aaagaattaa ttgtacttta aaggaaaata gaatgaatag actttcttgg gaagattatt
     3481 ttatggcaaa tgcagagttg atttctaaac gttctacctg tgaccgtgct tttgttggag
     3541 ctgttttagt caaaaataat cgcatcattg ctacaggtta taacggagga gtctctgaga
     3601 cagataactg taacgaagtg ggacattata tggaagatgg tcactgtatt aggactgttc
     3661 atgctgagat gaatgccctt attcagtgtg ctaaagaagg tatttctact aataatacag
     3721 aaatttatgt gacgcatttt ccttgtatca attgcaccaa ggcgctcttg caagccgggg
     3781 ttaagaaaat tacttataaa gctaattatc gcccacaccc atttgctatt gaacttatgg
     3841 aagcaaaagg agttgcttat gttcaacatg atgttccaga ggtaacacta gggatggaag
     3901 agtaaagagg aagatagagc atatataaaa tttattaaga ggaagtgact taatgcatag
     3961 atctttttta gaagaaaatt acttggaagc gatttatatc ttacaacaaa gaaatgaaag
     4021 tgttagaagt attgatgtag ccgaatactt gtctgttgct aaatcttcgg tgaactcagc
     4081 agtaaataaa ttactcactc agcaatatat aacctttgaa gatgataaga ttataaggat
     4141 gaccgctgag ggagaaaaaa tagcggaatt tgtttatgaa aaacatctat atttaaaaaa
     4201 attactatgt aagatgggaa ttgacgaaac agaagcagat gaaatagctt gtcttctaga
     4261 acacatttta aaagaagaca gctttaaaaa aataaaaaaa tttgtccaag acaaaaaaat
     4321 tatttaagaa aagtaaagtt gaaatatcag gttagaaagt acatctaaac taccttatct
     4381 gtggataatt taaaaacaaa caaagaaaag tattgatatt tgttctcaga tatgtacaat
     4441 atagttagat agaactaacc ggatattttt ttgcccttta ggttagatgc acctaactag
     4501 aaaattcaaa gaggaaattt gatggaaaca tatattgtta actatgataa aaaatggtga
     4561 tagcatagct gttgtaaacg gagacataga gttgtcttac aaggatattg ataggcagtc
     4621 aaactctttt gccaatggac ttttaaaagc tggcttcaaa tgtggagata aaatagtttt
     4681 acaattaccg aattatcacg agtttataat aattgctttc gccatgttta aagtaggcat
     4741 tatcccgatt atgagtttgc ctgctcatag aaaaaacgaa ctaaaaggga taatagaaaa
     4801 atcggaagct gtggcataca tagcaaaaga taagtaccta ggcttttctt atgttgatat
     4861 gatacgagat ataaagatag agtcagacag agaatttaaa gtttatatac ttggtgaagg
     4921 agatgaattt aaaaaatttt ctgaattgaa tgataataat tatatctatc aaaaaaccga
     4981 ggttgattat aaaaacatag gactacttct tttatccgga ggaacaacca gaataccaaa
     5041 gctgattccg agaaggcact gtgattatct gtatgtagca aagaaaacat ctattataag
     5101 cggtatggat agaggcagta tatacttagc agccttgccg atggcacata attttccttt
     5161 gtgttgcccc ggcattctcg gaacttttgc tgtaggtggg aaagttgttg tatgtgaggt
     5221 aaccagtcct gatgaaatac tacctctgat agaggaagaa aaggttacaa taacgggttt
     5281 tgtgccagcc atcgcaaata tttgcatgga ttatttacag tatgaagatt atgatctttc
     5341 atcattaaag atattgtaaa tcggtgggtc tgttttagag ccttggttag cggaaaagat
     5401 tgaagaaaca tttaatgtaa agttacaaca aatattcggt atagctgaag gtcttatttt
     5461 gacaacgagc atagatgatg acgaaaaaac cgtatggcaa acacagggta agccaattag
     5521 cgaacctgat gaaatactca ttgtggataa agcgagaaaa ttaaaagatg gcaattatca
     5581 aattgccgga agaattaaag agataatcaa cagagcgggc gaaaagatta caccactcga
     5641 attggaagaa atcttattaa cacatgattg cataaattcc gttcaagttt taggagtgcc
     5701 tgataaatta catggcgaag caattgccgt attcatttta aaaagcgaaa gcaagaggct
     5761 tacacttgaa gaagtaagga aatttttaat atcaaataac gtagcagact tcaaactacc
     5821 tgatgcggtt aagtatatag atagttggcc gttaacagcg ttaggcaaaa tagatagaaa
     5881 caaattaaaa gaaaattttt agaggagaac ttgaaatgga acttaacggt gtaaaagaaa
     5941 atgccaaaat attagtaaaa gattggctta aagatatatt tgctgaagaa gtaatttctg
     6001 aagatgaaaa tttaatagaa aaaggattaa gctctattaa aattatgcaa acaatattcc
     6061 gatatgcata agtgttcaaa atcaaatgac ttctaaaaac ccggacaatt atatgttctt
     6121 aatgcacaaa atttccctta caacaaattc aggaagttta acattatccg ggacaaacgg
     6181 tcctataata tgggagccgt gtttagataa accaacagat gaaaataaca gatttaattt
     6241 agaaaaaaat gagttttcgg aattaaaaat atttgaaata acggaggagg ttgaagaaac
     6301 atataacgat atgatgaaat taagttgggt agaggccata tctaaatccg tgattgactt
     6361 caccaacaat atcgaggcag aaaaagttga cttaagagaa cagcaatatt tgatttcagc
     6421 aatagaggca tggagagcat tatctaggga gttgggtcaa agtaatacca ttcaacctta
     6481 caaaaaaact gcaattaaaa tggaagattt aatataagga ggaaattatg aataacaaag
     6541 aaaacaaatt ataggtgaaa gatttaataa tgattggtgt attttccgca ttatacttct
     6601 tgtttaatat ggtaggagga ataccttttg ctatgaatcc ggtgctaaca ttttatcaac
     6661 cgatgggaag tgcattttta tcaggaataa tatttatgtt tttgatcgca aaagcaccta
     6721 aaaaaggaac aatttctatt ttagcaatca tcatgtgtat tttaaggctg gcaacaggaa
     6781 tgcattgggc tatggcggta ggaactttaa taacaggaat agtagctgag cttattgcag
     6841 gaagtaagag ttataaaaac aagaaaatga acatgttgtc ttttggagtt tttgcacttg
     6901 gagatatcgg aacattttta gtgtatttta tgaatcctaa gtcttggtca gatgcgatga
     6961 taaaaaaagg gacagatatc acctacattg agtctatgaa tgctgcagct gctgattgga
     7021 tgatttatgt aattgttatt ggaactttct tggttgcttt attaagtgct tggattggaa
     7081 tgaagctttt gaaaaaacaa tttgaaaagg ccggcattgt ataatgagaa aagtatcaaa
     7141 tgttcaattt aacccttgca caaaaatagt tttactattt ttgtgcataa tcatttcaag
     7201 tttattatct tcaatagaat tggagctaat ttttgtattt ataattgcgt tattttgcat
     7261 tataaacaaa aagattaaat ttgctcttaa tgggtttctg atttacctag gtgtatattt
     7321 tgttagcatt ttgactgtgc agtacggtag caatacctta agaagcttat taatgccttt
     7381 tctaggttta gtacataagc tttacccggt gtgcatgttt tctgtgctaa tactaagtac
     7441 tacaaaagta ggtgaatttt tatctgctat ggaatttgca ggtgtttcta aaaaaatcac
     7501 aattccttta gctgtaatgc ttagatatat tccaaccgtg cgtgaagact ggatatatat
     7561 aaaggattca atgtatttaa gaagtatctc gccttctttt ttcggtttta taaaaaatcc
     7621 ggatctaaca atagagtgta tttattcacc tataattatt atggcttcta aagcagcaga
     7681 cgaattaact atcgcttcta ttacaagagg aattgaatct cctaaaaaac gaagttcaat
     7741 tattgaaatt cgttttgata ttcaagacgt tttagcttta ctgattggag tggtaattac
     7801 tgtatttgtg atatatagga ggtttttctg tgattgaatt aaaaggggtt aatttcaaat
     7861 ataaaaacac caaaaacagc tgcttagaca atatagattt ggaaataaaa aagggagaat
     7921 tcgtcttaat ttccggtgct tcaggttctg gaaaaacatc tataacgaga gttataaata
     7981 agctgattcc ttattattac gaaggtgaat tagaaggcac agtaaaaata aacaacgatt
     8041 tagtatctag ctaccaaatg tttgagcttt cagaaatagt aggtagtgtt tttcagaatc
     8101 ctagaacaca atttttcaat gtggacacaa atagtgaaat agtttttggt ttagaaaatc
     8161 aaggggtttc tagagacatt ttaaaaaaaa gattaaaaga gacttgtgag acgttggata
     8221 taaacaaatt gcagaatagg aatatctttc atctgtcagg aggggaaaag cagaagattg
     8281 cttttgcgtc ggtgtatgca atgaatcccg atatttattt actggatgaa ccctcatcta
     8341 atttggatat accaacgata gaggtgctaa aaagacattt gtctgtttta aaaaacagtg
     8401 gcaaaacggt tattatatct gaacatcgaa tctactattt gatggatttg gttgacaaag
     8461 ttatatatat gaaaaatgga caaattcaaa aaagttattc aaaagaagat tttttgaatt
     8521 tgtctgaaga tgaaattaaa gctatggctt taagatgcag aaaaaaaaca aaactgaata
     8581 tagaaatgtc taataatgaa aaacatccat atttacaagt gaatgatttt tctatataca
     8641 ggaaagataa ttgcattata aataacatga acttttctgc gaacaaaggc gatattgttg
     8701 cggttatagg ttcgaatggt gttggtaaaa caacattttt aagaactctt tgtggtctgc
     8761 atgacaacta taaaggtgat gtgttgttga attctaaaaa aacagatgca aaaaaaagaa
     8821 gacaaaaaaa ctatatggtt atgcaagatg tgaattatca attatttgcc gagagtgtct
     8881 ttgctgaatg taaattagga atagaaaatg tgagcgatga tcttatagaa gaaatactaa
     8941 atgaatttga tttgagcgac tataaaaata atcatccaaa caccttatcc ggaggacaaa
     9001 aacaaagatt ggcaatagcc gtaggactaa tatgcaaaaa agaaattttt gtgttagatg
     9061 aaccaactag tggacttgac tatacgaata tggtaaagac tgtagaattg ttaaaacgtt
     9121 attccaaaga caaaattatt tttattgcta cgcatgatac cgaatttgct aatttgattt
     9181 gtaatcgggt attggactta gaaaaataga tggaaaggag gactttaatg tggacgtttt
     9241 aagaagatta tataagttcg cacctgaaaa aaaggtgtac tcctatgcgt cgctagtttt
     9301 atctgcaata gctactatat tctcagtagt cccatatttt tatttatgga agtttttgaa
     9361 tgaactttta gtacttaaaa accttgaaag tgcaaataaa tatgcgttat ggatttttat
     9421 gtttttggtt ttacaaacat tagtgtattt tttgagttta tattgtagcc acatgtttgc
     9481 ttttagagtc gaaagaaatt taaaaataga aggcttaaac aaccttttag aggcatcttt
     9541 ttcttttttt gatacaaatc catcaggaaa aactaggcaa ataatagacg ataatgcgtc
     9601 ggaaacacat tccatacttg ctcacatgca accggacatg gtcaatgcta tggtttatcc
     9661 tgtaattttg ctggttgtaa gttttttggt aagtataagt tttgggatag taatggttgt
     9721 atcattagtt ttaggcgtat ttttaatcac gaaaatgttt gggaatcaaa attttctcca
     9781 aaaatatatg gcttccaatg aagatatgag ttccgaaata gttgaatata taagaggtgt
     9841 caaggttatc aaaatattca atataggaat agaaaaattt cgttctttgc ataaaacaat
     9901 catcacctct tcagaactag gttatgcgta cgctatctcg tgtagaaaat ggaaggtttt
     9961 ttacgattca tttttcatgg ggctatgcat ggttacgata ccttatggtg tatatttgct
    10021 aaacattaat acgcatctag gagaaattat ttcctacacc gtattttcac agcatttatt
    10081 gaattgttat acaacgcttt taataaagtg atgtttttgg gacaaaataa ggctaaagcc
    10141 aaaaactcta tagataagct ggaagaaatt tttaacaaaa tgcaggtaaa aaaattagat
    10201 agtgggcttt cagatgaaat acaaaatttt gatattgaat ttgaaaaagt atcgtttaaa
    10261 tacgaaggaa atacaccata tgttttaaaa gacttatcgt ttaagttgga ttcgggaaaa
    10321 agttatgcac ttattggttc atctggcagt ggaaaatcca caattgctaa gttaatttcc
    10381 gggttttatg atgttgattc cggagtaata aaaattggtg gacgagatat taaagagtat
    10441 tcaagggaaa cgatagcaag tagtatcgct tttgttttcc agaatgcgaa attatttaaa
    10501 atgagtattt atgacaacgt gagaattgga aatcctgatg ctacttatga agagattatg
    10561 caagctcttg aaaaggctag gtgcaatgaa attttggata agtttgagct gagggaaaat
    10621 acaatgatcg gagcagaagg cgtttatctc tcaggaggag aaactcaaag aatagtaatt
    10681 gcaagagcaa ttctaaaaaa ttcacccatt ataatacttg atgaggcaag tgcagctgct
    10741 gatccggaaa atgaatatga actccagatg gcatttacgc aattgataaa aaataaaaca
    10801 gtaataatga ttgctcacag gctgtcagca attaaaaaca tggacgaaat catagttgta
    10861 gaaaatggac agattataga aagaggaagt agcaaacttt tattagagga taaaagctct
    10921 aaatacagct attttatgaa tatgtatatt cgagcaaatg attggataat gtctaatgaa
    10981 gtctaaatta atggataaat acggaataac agaagacagc tcaatagatc taattaaagc
    11041 ttcgatagcg tcgttctgga aaaatgtagt atacatgttc cctataacgc ttatattatt
    11101 ttttctcatg aatataagca actcgaaata tctacaagag tggttttatt ttgtaagcat
    11161 attaattgtc tttgttgtaa tgtactattt tacaaataga caatatataa aatcgtattc
    11221 tacaacattt aagggaagta aaaatttaag aatagaactt gtagaaataa taaaaagact
    11281 accgttatca tatttttcaa cacatgattt gacagattta tcgcaaacag taatggctga
    11341 tgtgtcaaaa atcgaacaag ccttatctca tgctatatgt gcgtattatg gatggattgg
    11401 ttattttgtt tttctaagtt tgagcctttt aattggatct ttacaattag gactatgtat
    11461 tattgtacct gtaataactg cattgctgat attgtatctg tcgaaaaata ttcaatatag
    11521 aataaataga aagtattata aaaaactgag agagaattct caggcatttc aagattcttt
    11581 tgagatgcaa caagatataa aatcttataa catgcaacat acagtgagaa aagatgtaca
    11641 agacaaattg aaagacagtg aaaaaataca gataaaaaca gagctgttta caacgatacc
    11701 atcaagctta ttatcaattt tgccacagtt ctcgatagct ctggttatta ttttcgggtt
    11761 tcaactgtat ttgactggtc aaatacagtt aatcttctat ataggatata taatttgtgc
    11821 cacaaaggta gccagtggtt ttacgggcat gttagacagt cttttaatga taataaactt
    11881 tcaaagcagt tttaaacaag ttacggcaat aagaaagact cctttacaag aaggggtaaa
    11941 tgcagatatt cagacattca atgttgaatt tagggatgtt agctttgctt atgagaaagg
    12001 aagaaatgtt gtaagtaatt tatctttcaa tgcaaatcaa ggagaagtta ctgcgatagt
    12061 agggccctcc ggttgtggaa agtcaactat tttaaaacta ctatcaagat tatacgacta
    12121 taatgccgga caaatattga taggcgggaa agatatttct gaaatatcaa caaatagttt
    12181 atttgaaaat atttcaacag tcttccaaga tgttgagtta tttgatgata ctatttttga
    12241 aaacattaaa tgtggaaatc ccaaagctac tatcgaagaa gtgttagaag ctgcgaaaat
    12301 tgcaaatgta gatgagattg ctgataaatt cgtagataaa tatgacacaa aaataggtga
    12361 aaacgggagt aaattatcag gaggcgaaag acaaagaatt tctattgcaa gagccattct
    12421 taaagatgca ccaataatac tgcttgatga aatctctgcc agtttagata tagaaaatga
    12481 aataaagata caagccggtt tagataagct tataaaaaat aaaacagtta ttatcgtttc
    12541 tcatagaatg aagtctatag aaaacgcaaa taagattata gttattgaaa acggaaaggt
    12601 agagaatttt ggaactcata gctatttatt ggaaaattcg acgagataca gaaaa
//
LOCUS       DBJYSH010000061        12329 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.061, whole genome shotgun sequence.
ACCESSION   DBJYSH010000061 DBJYSH010000000
VERSION     DBJYSH010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 12329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12329
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.061"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            complement(197..763)
                     /locus_tag="ACUBV3_001287"
     CDS             complement(197..763)
                     /locus_tag="ACUBV3_001287"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000596040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (mnm(5)s(2)U34)-methyltransferase"
                     /protein_id="HHU3847791.1"
                     /translation="MIKRPIHLSHDFLAEVIDKEAITLDATMGNGNDTVFLAKSSKKV
                     YAFDIQEEAIAKTKAKLTEQGISNAELILDGHENLEQYVHTPLRAAIFNLGYLPSADK
                     TVITKPHTTIKAIKNVLDILEVGGRLSLMVYYGHDGGKSERDAVIAFVEQLPQNNFAT
                     MLYQPLNQVNTPPFLIMVEKLQSYENEV"
     gene            complement(756..1691)
                     /locus_tag="ACUBV3_001288"
     CDS             complement(756..1691)
                     /locus_tag="ACUBV3_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747785.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HHU3847792.1"
                     /translation="MKKRYRAINDYYRELFGEKIFKLPIDAGFDCPNRDGTVARGGCT
                     FCTVSGSGDAIVAPEAPIREQFYKEIDFMHRKWPEVNKYLVYFQNFTNTHAKLEIIKE
                     RYEQAINEPGVIGINIGTRPDCLPDETIYYLAELSERMHVTLELGLQTTYEATSALIN
                     RAHSYDLYKKTVKRVRELAPKVEIVSHLINGLPGETHDMMVENVRRCVTDNDIQGIKL
                     HLLHLMINTRMQRDYHEGRLRLLSQEDYISIICDQLEIIPKHIVIHRITGDAPRHMLI
                     GPMWSLNKWEVLNAIDKEMEKRQSYQGCKVEVKHD"
     gene            complement(1784..2425)
                     /locus_tag="ACUBV3_001289"
     CDS             complement(1784..2425)
                     /locus_tag="ACUBV3_001289"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HHU3847793.1"
                     /translation="MLSRQNSKLIQAFIAIILFFSLGLVIKYWPDTVIAFDQTIQESV
                     RGQLPNLSTRFFKLITVVGNTVSQIAIAIMSVTFCYLKKWYPQARFIAVNAIISGICI
                     LSLKLIFQRVRPTLTHLVFAGGYSFPSGHSMGTFMIFGSSIILLQYYMPKSIWKLLCQ
                     GTLGLLIFLIGLSRIYLGVHFPTDVLAGFILAYGILNLTAYIFLSKDWLKKVK"
     gene            complement(2406..2990)
                     /locus_tag="ACUBV3_001290"
     CDS             complement(2406..2990)
                     /locus_tag="ACUBV3_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HHU3847794.1"
                     /translation="MTNTRKMVTIAILSALSFVLMMVSFPLIPGAEFLKVDFSILPML
                     VAFILFDLKSSYGVLLLRSLLKVILANRGPETFIGLPMNMVALALFLASFAIFWKNRE
                     SAKDFIKASLFGTVSLTVSMVALNYVFAIPLYAIFANFDIRTFIGVGNYLLTMVIPFN
                     IVEGILISIVFYLTYVACLPILERYKKTNVISSK"
     regulatory      complement(3059..3204)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(3273..3872)
                     /locus_tag="ACUBV3_001291"
     CDS             complement(3273..3872)
                     /locus_tag="ACUBV3_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000621247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HHU3847795.1"
                     /translation="MIRRYSANFLAILGIILVSSGIYWGWYNINQAHQADLTSQHIVK
                     VLDKSITHQVKGSENGELPVKKLDKTDYLGTLDIPNLKLHLPVAANYSFEQLSKTPTR
                     YYGSYLTNNMVICAHNFPYHFDALKNVDMGTDVYFTTTTGQIYHYKISNREIIEPTAI
                     EKVYKTATSDNDWDLSLFTCTKAGVARVLVRCQLIDVKN"
     gene            complement(3865..4536)
                     /locus_tag="ACUBV3_001292"
     CDS             complement(3865..4536)
                     /locus_tag="ACUBV3_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3847796.1"
                     /translation="MIYKKILKITLLLLFSLSTQLVSADTNDQMKTGSITIQNKYNNQ
                     GIAGGNLLVYQVAQAKDVDGNQVFTLTTPFQGIGIKDDDLTQVNLDSNQAKYVNLLTK
                     AVHKTQPLQTFDNLPAEGIVANNLPQGIYLFIQTKTAQGYELMSPFILSIPKDGKYDI
                     TAFEKMSPLNAKPKKEETITPTVTHQTKGKLPFTGQVWWPIPILIMSGLLCLIIALKW
                     RRRRD"
     gene            complement(4526..5401)
                     /locus_tag="ACUBV3_001293"
     CDS             complement(4526..5401)
                     /locus_tag="ACUBV3_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HHU3847797.1"
                     /translation="MKKIRQHLVSILLVTIIAVGVGLMAYPSLANYWNSFHQSRAIMD
                     YQDRVTHMDENDYKKIINRAKEYNKQFKTSGMKWHMTSQERLDYNSQLAIDKTGNMGY
                     ISIPKINIKLPLYHGTSEKVLQTSIGHLEGSSLPIGGDSTHSILSGHRGLPSSRLFSD
                     LDKLKVGDHWTVSILNETYTYQVDQIRTVKPDDLRDLQIVKGKDYQTLVTCTPYGVNT
                     HRLLVRGHRVPNDNGNALVVAEAIQIEPIYIAPFIAIFLTLILLLISLEVTRRARQRK
                     KILKQAMRKEENNDL"
     gene            complement(5439..6947)
                     /locus_tag="ACUBV3_001294"
     CDS             complement(5439..6947)
                     /locus_tag="ACUBV3_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HHU3847798.1"
                     /translation="MKKKMIQSLLVASLAFGMAVSPVTPIAFAAETGTITVQDTQKGA
                     TYKAYKVFDAEIDNANVSDSNKDGASYLIPQGKEAEYKASTDFNSLFTTTTNGGRTYV
                     TKKDTASANEIATWAKSISANTTPVSTVTESNNDGTEVINVSQYGYYYVSSTVNNGAV
                     IMVTSVTPNATIHEKNTDATWGDGGGKTVDQKTYSVGDTVKYTITYKNAVNYHGTEKV
                     YQYVIKDTMPSASVVDLNEGSYEVTITDGSGNITTLTQGSEKATGKYNLLEENNNFTI
                     TIPWAATNTPTGNTQNGANDDFFYKGINTITVTYTGVLKSGAKPGSADLPENTNIATI
                     NPNTSNDDPGQKVTVRDGQITIKKIDGSTKASLQGAIFVLKNATGQFLNFNDTNNVEW
                     GTEANATEYTTGADGIITITGLKEGTYYLVEKKAPLGYNLLDNSQKVILGDGATDTTN
                     SDNLLVNPTVENNKGTELPSTGGIGTTIFYIIGAILVIGAGIVLVARRRLRS"
     gene            complement(6988..11292)
                     /locus_tag="ACUBV3_001295"
     CDS             complement(6988..11292)
                     /locus_tag="ACUBV3_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="InlB B-repeat-containing protein"
                     /protein_id="HHU3847799.1"
                     /translation="MLKKCQTFIIESLKKKKHPKEWKIIMWSLMILTTFLTTYFLILP
                     AITVEETKTDDVGITLENKNSSQVTSSTSSSQSSVEQSKPQTPASSVTETSSSEEAAY
                     REEPLMFRGADYTVTVTLTKEAKIPKNADLKVTELKDNSATFKDYKKKALTEVAKQDS
                     EIKNFKLYDITIESNGKEAEPQAPVKVEVNYDKPLEASDENLKVVHFKDDGQTEVLKS
                     KDTAETKNTSSDVAFKTDSFSIYAIVQEDNTEVPRLTYHFQNNDGTDYDFLTASGMQV
                     HHQIIKDGESLGEVGIPTIKAGEHFNGWYTYDPTTGKYGDPVKFGEPITVTETKEICV
                     RPFMSKVATVTLYDDSAGKSILERYQVPLDSSGNGTADLSSFKVSPPTSTLLFVGWSK
                     TQNGAPLSESEIQALPVSSDISLYPVFKESYGVEFNTGDLSTGVTYIAPRRVLTGQPA
                     STIKPNDPTRPGYTFAGWYTAASGGAAFDFNQVLTKDTTLYAHWSPAQTTYTINYWQQ
                     SATDNKNATDAQKTYEYAGQVTRSGLSLSNQTLTQQDINDKLPTGFKVNNTRTETSVM
                     IKDDGSSVVNVYYDRKLITIKFAKYGGYSLPEYYYSYNWSSDADTYTGLYGTTLAANG
                     YQWKTGAWGYLANVGNNQVGTYGMSYLGEFILPNDTVDSDVIKLFPKGNIVQTYRFFK
                     QGLDGTYSLADTGGGAGADEFTFTEKYLGFNVKYYQRLYPDNYLFDQYASQTSAGVKV
                     PISDEYYDRYGAYHKDYLNLVVWYERNSYKIKYLDPLDNTELPNFPVKDVLYEQNLSS
                     YAPDTTTVQPKPSRPGYVWDGKWYKDQAQTQVFDFNTTMPPHDVKVYAGWQKVTYRVN
                     IDPNGGRLSKTDDTYLDLHYGDRIPDYTDITRDYIQDPSGTYYYKYDSRDKDPDSTKD
                     AYYTTDTSLSNVDTTTKYKYVKDAYKLVGWYYVNPDGSIRPYNFSGAVTQDINLRAIW
                     RKAGDYHIIYSNDAVGTDGKPALDASGQQLQTSNEPTDPDSYDDGSHSALLRRPTMPD
                     GYRFRGWWYNGKIYNPYDSIDIDAHLADANKNITIKPVIIPVGDIKLEDTSIKYNGNG
                     GTRVENGNVVTQVETPRMELNSTTTIPENQYFTRTGYNLIGWHHDKDLADTGRVEFTA
                     GQSIGIDNNPDATNTLYAVWQPKEYTVRVSKTVVGLDEDKTKDFLFNPSETLQQENFP
                     LRDGQTKEFKVPYGTSISIDEQAYDEFKVSESITEKNLATGEADKTYDATGLQSLTVS
                     GDVDISFTNTRIKQKVRLQKVNVENDNNFLAGAVFDIYESDANGNKASHPMYSGLVTN
                     DKGLLLVDANNYLSLPVGKYYLTETKAPPGYLLPKNDMSVLVISTGVTFEQNGNNATP
                     IKENLVDGSTVYTFKITNSKGTELPSTGGIGTHIYILVGLALALPSGLILYYRKKI"
     gene            complement(11312..11881)
                     /gene="lepB"
                     /locus_tag="ACUBV3_001296"
     CDS             complement(11312..11881)
                     /gene="lepB"
                     /locus_tag="ACUBV3_001296"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HHU3847800.1"
                     /translation="MKRQISSDKLSQELDRVTYQKRFWSVIKNTIYILMAVASIAILI
                     AVLWLPVLRIYGHSMNKTLSAGDVVFTVKGSNFKTGDVVAFYYNNKVLVKRVIAESGD
                     WVNIDSQGDVYVNQHKLKEPYVIHKALGNSNIKYPYQVPDKKIFVLGDNRKTSIDSRS
                     TSVGDVSEEQIVGKISFRIWPLGKISSIN"
     gene            complement(11865..12137)
                     /locus_tag="ACUBV3_001297"
     CDS             complement(11865..12137)
                     /locus_tag="ACUBV3_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3847801.1"
                     /translation="MEDLKKLKRKDLLEIMLEQQLLIEKQEKEIQQLKEALDDKKIKI
                     SKAGSIAEASLMLSGIFETAQLAADTYLENVKNLVDKRGVDNEKTD"
BASE COUNT         3755 a         2356 c         1972 g         4246 t
ORIGIN      
        1 tttttttgcg atttttaact ctgaaaacct atgaaatcaa gtattataaa aaccaacgga
       61 acttagtctg agacggattt tcgttggttt ttattggttt ttatcagact aaattccatt
      121 tctactagag gtggaactta ctttgagaat ttagctttta tagcaattat catttttgct
      181 aattgcctaa agatagttac acctcatttt catatgattg taacttttct accataatta
      241 aaaagggggg cgtattaact tgattgagtg gttgataaag cattgtagcg aaattattct
      301 gtggtaattg ttccacgaat gctatcaccg catctctctc actttttcca ccatcatgac
      361 cataataaac cataagtgat agacgtccac ctacttctaa aatgtctaaa acgtttttga
      421 ttgccttgat agttgtgtgt ggttttgtaa taactgtttt atccgctgag ggtaagtatc
      481 ccagattaaa tatagccgct cttaaaggtg tatgaacata ctgttccaaa ttttcatgcc
      541 catccaagat aagttcagcg ttagagatcc cttgctccgt gagctttgct ttcgtttttg
      601 ctattgcttc ttcttgaata tcaaatgcat aaactttttt agaacttttc gctaaaaaaa
      661 cagtatcatt cccatttccc atagttgcat caagcgttat tgcttcttta tcaataactt
      721 ctgctaaaaa atcatgagaa agatgaattg gtcttttaat catgttttac ctcaacttta
      781 catccttgat atgactgtct tttctccatt tctttgtcaa tggcattaag cacttcccat
      841 ttattaaggc tccacatcgg tccaattaac atatgacgtg gcgcatcacc agtaattcgg
      901 tgaataacaa tatgttttgg aataatctcc agttggtcac aaataatcga aatatagtct
      961 tcttgactta acaaacgtaa acgaccttca tggtaatctc tctgcattct tgtattaatc
     1021 ataagatgca gaaggtgtaa ttttataccc tgaatatcat tatccgtaac acatcgacga
     1081 acattttcca ccatcatatc atgtgtctcc cctgggagac catttattag gtgagaaacg
     1141 atttctactt taggagctaa ttctcgtact ctcttaacag tttttttgta aaggtcatat
     1201 gaatgtgctc tatttattaa tgcagaagtt gcttcataag ttgtctgaag gcctaattct
     1261 aaagtcacat gcattctttc agaaagttca gcgagatagt atatagtttc atcaggtaag
     1321 caatccggcc ttgttccgat gttgatcccg ataactcctg gctcattaat agcctgttcg
     1381 taacgctctt taattatctc taacttagca tgggtattgg taaaattttg aaaatagact
     1441 aagtatttat taacctcagg ccactttcta tgcatgaaat caatttcttt atagaattgt
     1501 tcacgaatag gagcttctgg agcaactata gcatcccctg aaccagaaac tgtgcaaaaa
     1561 gtgcaccctc ctctagcaac tgttccatct ctgttaggac agtcaaaacc agcatctata
     1621 ggtaatttaa atattttttc tccaaagagt tctcgataat aatcattaat cgcacgataa
     1681 cgttttttca taggataatt gtatcacaat tttaactaaa ataacctcac tactacaata
     1741 aaactaaaaa agattggaac gtcagttagt cccaatcttt tatttacttc actttcttta
     1801 accaatcctt ggataaaaag atatacgcag ttagattcaa aataccataa gcaagtataa
     1861 aaccagctaa aacatctgtc ggaaaatgaa cccctaggta aatacgagat aacccaatta
     1921 aaaaaatgag caaacccaat gtaccttggc acaacagttt ccatatactc ttaggcatat
     1981 agtactgcaa taaaataata ctactcccaa atatcataaa tgttcccatc gagtgcccac
     2041 tgggaaacga atagccacct gcaaatacta aatgggttaa agttggtctc actctttgaa
     2101 aaataagttt taaagaaagt atacatatac cagagataat agcatttact gcgataaatc
     2161 tagcttgagg ataccacttc ttaaggtaac agaaagtgac gctcataatc gcaatagcta
     2221 tctggcttac agtattacca accacagtga ttaacttaaa aaatcttgta gaaagatttg
     2281 gcaactgtcc tctaacactt tcttgaatag tttggtcaaa tgcgattaca gtgtcgggcc
     2341 aatatttgat gaccaatcct aaactgaaaa ataagataat agcaataaat gcttgaataa
     2401 gtttactatt ttgacgagat aacattagtc tttttatatc tttctaatat tggcaaacaa
     2461 gccacgtaag ttagatagaa aacaatcgaa attaaaattc cctcaacgat attaaatgga
     2521 ataaccattg ttaaaaggta attgcctaca ccaataaatg ttctgatatc aaagttagca
     2581 aatatagcat acaaaggaat cgcaaagaca tagttgagag ctaccataga tacagtcaag
     2641 ctaactgtac caaataaact agctttaata aaatcttttg cactctctct atttttccag
     2701 aaaatagcga aacttgctaa aaatagagct agagcaacca tattcatcgg taaaccgata
     2761 aaggtttctg gaccacgatt agcaagtata acttttaaaa gtgatcttaa taagagtaca
     2821 ccataacttg atttcaaatc aaataaaata aaagcaacta acatcggaag gattgaaaaa
     2881 tcaaccttta aaaattctgc tcctggtatt aatggaaatg aaaccatcat caatacaaaa
     2941 gataaggcag aaagaatggc gattgtcacc attttacgtg tatttgtcat aaaaaaattc
     3001 ctccaattta aataaattga aagaagctcc aaaggtaagc gtaggtacgc gaaaaaacct
     3061 ttgtcttctc ccatccagac tttactgtcg gttgtggaat ctcaccacat cagctttcgc
     3121 tcgcggactg atgcttcaca actgacaaat aagttggaag cgattaccgc cggtcgggaa
     3181 ttacaccctg ccctgaagac acctatagca taacaaaaaa aacttgcaat tgcaagtttt
     3241 ttaatcacta attagtagta gattgtataa tattaatttt taacatcaat taattgacag
     3301 cgcactaata ctctagctac tcctgccttt gtacaagtaa acaagcttaa gtcccaatca
     3361 ttgtctgatg tggcagtttt ataaactttt tcaatcgctg ttggttcaat aatttctcta
     3421 ttactgattt tgtagtgata gatttgccct gttgtagttg taaaataaac atccgttccc
     3481 atatctacat tttttaaagc atcaaaatga taaggaaaat tatgcgcaca aatcaccatg
     3541 ttattagtta aataagaacc ataatacctt gtaggcgtct tagacagttg ttcaaaacta
     3601 taattagcag ctaccggtaa atgcagtttt aagttcggaa tatccagagt tcccaagtaa
     3661 tctgttttat ccaacttttt tacaggtaat tctccatttt ctgaaccctt tacttgatgc
     3721 gtaatagatt tatcaagcac cttgacaata tgctgagaag ttaaatcagc ttgatgcgcc
     3781 tgattaatat tataccaacc ccaatagatt ccagaactta ccagaataat tccgagtata
     3841 gctaaaaaat ttgctgaata tcttctaatc acgtcttctt ctccatttta aagctattat
     3901 taaacacaga agtcctgaca taattagtat aggtattggc caccatactt gtccagtaaa
     3961 cggaagcttt ccctttgtct gatgtgttac tgtaggagta attgtctctt cttttttagg
     4021 tttagcattt aaagggctca ttttctcaaa tgctgtaata tcgtacttcc catccttagg
     4081 tattgatagt ataaagggag acattagttc ataaccttga gccgttttag tctgaataaa
     4141 tagataaatc ccttgaggaa gattgttcgc aacaatacct tcagccggta aattatcaaa
     4201 cgtttgtaaa ggttgagttt tatgaacagc ttttgttagt agattgacgt atttggcttg
     4261 gttactatca aggtttactt gtgttagatc atcgtctttt attccaatac cttgaaatgg
     4321 ggtagttaga gtaaaaactt gattaccatc aacatcttta gcttgtgcta cttggtaaac
     4381 aagtaaatta ccgccagcga taccttgatt attatactta ttttgtatag taatagaacc
     4441 cgttttcatc tgatcattgg tatcagcaga cacaagttga gtacttagac taaataataa
     4501 gagaagagtt atctttagga tctttttata aatcattgtt ctcttccttt ctcattgctt
     4561 gttttaaaat tttcttacgt tgacgtgctc tcctagttac ttctaaagag attaaaagta
     4621 aaatcaaagt aaggaaaata gcgataaatg gtgcgatata aataggctct atttgtattg
     4681 cctctgctac taccaaagcg ttaccattat cgtttggtac acgatgtcct ctcactagta
     4741 accgatgggt attaacgcca tatggtgtac acgtcaccaa agtttggtag tctttacctt
     4801 taacaatttg taaatccctc aaatcatccg gtttaactgt tctgatttga tccacttgat
     4861 aagtatatgt ttcatttaag atactgactg tccagtggtc tccaactttt aacttatcca
     4921 aatcagaaaa aagccttgaa gagggtaaac ctctatgtcc tgataaaata gaatgagttg
     4981 agtctcctcc aattggaaga ctacttcctt ctaaatgacc aatagaagtt tgaagcactt
     5041 tttcacttgt accatgataa agtggtaatt ttatgtttat ctttggaatt gaaatataac
     5101 ccatattacc cgttttatcg atagccagtt gtgaattata atccaaacgc tcttggctag
     5161 tcatgtgcca cttcattcct gaagttttaa attgcttatt atattctttg gctcggttaa
     5221 taattttttt ataatcgttt tcatccatat gcgttacgcg gtcttggtaa tccataatcg
     5281 ctcgagattg gtgaaatgaa ttccaataat tagcaagtga aggataagcc attaagccta
     5341 cccccactgc aattatagtg acaagcaaaa tggatactaa atgttgtctt atttttttca
     5401 tatattttaa atctgtacca ctttgctagc ccatcttatt aagaacgtaa acgacgacga
     5461 gcaacaagca cgatacctgc tcctattact aaaattgcac ctataatgta gaaaattgtt
     5521 gtaccaatac cacctgttga aggcaactca gtacctttgt tattttcaac agttgggtta
     5581 actaaaaggt tatctgaatt agtcgtatca gtggctccat ctcctaaaat aaccttctga
     5641 gagttatcta acaaattgta acctaaggga gcctttttct caactagata gtatgtacct
     5701 tctttcaagc ctgtaatggt aattatacca tctgctcctg ttgtatattc tgttgcatta
     5761 gcttctgtgc cccattcaac gttatttgta tcgttaaagt ttagaaattg acccgtagca
     5821 ttctttaaaa caaatatagc accttgtaat gaagcttttg tggaaccatc aatttttttt
     5881 atagtaattt gaccatccct cactgttact ttttgacctg ggtcatcatt gctagtattg
     5941 gggttgatgg tcgcaatgtt tgtattttct ggtaaatcag ctgaacctgg tttagctcca
     6001 ctctttaata ctcctgtata agtgactgtg attgtattta ttcccttata aaaaaagtca
     6061 tcattagctc cattttgagt atttccggtt ggagtattgg tagctgccca cggaatagta
     6121 atcgtgaaat tattattttc ctctaacagg ttatacttcc cagttgcttt ttccgaacct
     6181 tgagttagag ttgtaatatt ccctgatcca tcagtaatag ttacttcata agacccttcg
     6241 ttcaaatcaa ctacagaagc agatggcata gtatccttta taacatattg atacactttt
     6301 tctgtaccat gataattgac tgcattctta taagtaatag tatatttgac tgtatcacca
     6361 accgagtacg ttttttgatc tacagttttt ccaccaccat ctccccatgt cgcatcagta
     6421 ttcttttcat gaatagtagc atttggagtt acagatgtaa ccataattac agctccatta
     6481 ttaacagtgc tagaaacata ataatatcca tattgggaaa cattaataac ctcagtacca
     6541 tcattatttg actcagtaac agtggaaact ggtgtagtat tagctgatat agatttagcc
     6601 catgtcgcaa tctcatttgc tgacgcagta tcttttttag ttacatatgt tctccctcca
     6661 ttagtagttg tcgtaaaaag agaattaaaa tcagttgaag ctttatactc agcttcttta
     6721 ccttgaggaa ttaaataaga agctccatct ttattcgaat cagatacatt tgcattatct
     6781 atttctgcat caaaaacttt atatgcttta taggttgcgc ctttttgagt atcttgaact
     6841 gtaattgtcc ctgtctcagc ggcaaaagct atcggcgtaa ctggtgatac agccatacca
     6901 aatgctaaac tcgccactaa cagcgattga atcattttct ttttcattga aatctttctc
     6961 ctaaaatcat attgatgaat gattaattca tatttttttt cgatagtata atattaatcc
     7021 tgatggtaga gctaaagcta aaccaactag gatataaatg tgtgttccaa tacctccagt
     7081 actaggcaat tctgttcctt tactgttagt aattttaaaa gtatatactg tacttccatc
     7141 cactaaattc tcttttattg gtgtcgcatt attaccattt tgttcaaagg taactcccgt
     7201 agaaatcact aatactgaca tatcattttt aggtagtagg taccctggag gggcctttgt
     7261 ctctgttagg tagtattttc ctactggcaa actgaggtag ttattagcat ccactaataa
     7321 caagccttta tcgtttgtca ccagccctga atacatagga tgtgaagctt tattcccatt
     7381 agcatctgat tcataaatat caaaaactgc acctgctaaa aaattattat cattttcgac
     7441 attaactttc tgtagtcgta ctttttgctt gatacgtgta ttggtaaagc taatatctac
     7501 gtctcctgaa actgtcaggg attgtaagcc ggtagcatca taagttttat cagcttcacc
     7561 agttgctaga tttttttctg taattgactc agatacttta aattcatcgt aggcttgttc
     7621 atctattgat atagaagttc cataaggtac tttaaattcc ttagtctgac catctctcag
     7681 cggaaaattc tcttgttgca acgtttcact tggattaaac aagaagtctt tcgtcttatc
     7741 ttcatctagt ccaacgacag ttttacttac tctgacggtg tattctttag gttgccaaac
     7801 agcatataag gtatttgttg catcagggtt gttatcaata cctattgatt gacctgctgt
     7861 aaattccaca cgtcctgtat cagctaaatc cttatcatga tgccaaccaa taaggttgta
     7921 acctgtcctt gtaaagtatt ggttttcagg aattgtagtt gtgctattca actccatacg
     7981 cggtgtctcc acttgtgtta ccacattacc attttctact ctagtaccac cgttaccatt
     8041 gtatttgatg gaggtatctt ctaatttgat atctcctact ggaataatga caggttttat
     8101 ggtgatattt ttattagcat ctgctaaatg ggcgtcaata tcaatggaat catatgggtt
     8161 ataaatttta ccattgtacc accagccacg gaaacgatag ccatctggca ttgtcggacg
     8221 tctcagtaag gctgaatggg agccatcgtc ataggaatca gggtcagtag gctcattact
     8281 tgtttgtaat tgctgaccag aagcatccaa tgctggcttt ccatctgtac caacagcatc
     8341 attgctgtat ataatatgat aatctccagc ctttcgccaa atagctctta aattgatatc
     8401 ttgagttaca gcacctgaaa agttataagg tctaatacta ccatctggat taacataata
     8461 ccaaccgact aatttgtaag cgtcttttac gtacttgtac ttagttgttg tatcaacatt
     8521 tgagagacta gtatctgtcg tataataggc atctttagtt gagtcgggat ctttatctcg
     8581 tgaatcatac ttataataat atgtacctga aggatcttgg atataatccc ttgtaatatc
     8641 tgtataatcc ggaatacgat caccataatg caaatctaaa taggtatcat ctgtttttga
     8701 taatcggcct ccgtttggat caatattgac acgatatgtt accttttgcc aacctgcata
     8761 gactttaaca tcatgaggag gcatagtcgt gttaaagtca aatacttgtg tttgtgcttg
     8821 gtctttatac catttaccat cccaaacata ccctggtcga ctaggtttag gttgaacagt
     8881 tgtcgtatcg ggggcataag aggacaaatt ttgctcatat agaacatcct ttactggaaa
     8941 attaggaagc tctgtattat caagcggatc taaatattta atcttgtatg aattacgttc
     9001 ataccatacc actaagttca aataatcttt gtggtaggca ccatatctat catagtattc
     9061 atctgaaata ggaactttta ctcctgcact cgtttgactt gcgtactgat caaaaaggta
     9121 attatcagga tataaacgtt gataatattt aacattaaac cctagatatt tttctgtaaa
     9181 ggtaaattca tctgccccag cacctccacc ggtatctgct aaagaataag tgccatccaa
     9241 accttgtttg aagaaccgat aagtttgaac tatattccct tttggaaaga gtttgataac
     9301 atctgaatca acggtatcat taggtaaaat gaactcaccc aaataactca tcccataggt
     9361 tccaacttgg ttatttccaa cattggctaa ataaccccat gcacccgtct tccattgata
     9421 accattagcg gccaaggttg taccgtaaag tcctgtgtaa gtatcagcat cagagctcca
     9481 attatacgaa tagtaatatt ctggcaagga atatccgcca tattttgcaa attttattgt
     9541 gatgagtttt ctatcataat aaacattaac aacacttgaa ccatcgtctt ttatcatgac
     9601 agaagtttct gtcctcgtat tattaacttt aaagccagtc ggtagtttat cattaatatc
     9661 ttgttgtgtt agcgtctgat tagataaaga taggcctgat cgtgttactt gccctgcgta
     9721 ctcatatgtc ttttgcgcat cagtagcatt tttattatcc gttgctgatt gttgccagta
     9781 gtttatcgtg taggttgttt gtgcgggcga ccaatgtgca tatagcgtcg tatccttggt
     9841 caagacttga ttaaaatcaa aggctgcccc accactagca gctgtgtacc accctgcaaa
     9901 agtataacct ggcctcgttg gatcattagg cttaattgtc gaagcaggtt ggcctgttaa
     9961 cacacgacga ggtgcaatat aggtaactcc tgttgataag tcgcctgtgt tgaattcaac
    10021 accgtacgat tctttaaaga caggataaag acttatgtct gaagatacag gtaatgcttg
    10081 aatttctgat tcagaaagtg gagctccatt ttgagtttta gaccaaccta caaataacaa
    10141 tgttgaagtg ggtggtgaaa ctttaaatga acttaaatcc gcggttccgt ttcctgaact
    10201 atccaatggt acttgataac gctccaaaat acttttacca gcagaatcat catataaagt
    10261 tactgtcgcc actttactca taaatggacg aacacaaatt tcttttgtct cagttacagt
    10321 tattggctca ccaaatttaa cagggtcacc atactttcca gtagtaggat cataggtata
    10381 ccaaccatta aaatgctctc ctgctttaat cgtcggaatc ccaacttctc ctagagattc
    10441 tccatctttt ataatttgat gatgaacttg catacctgaa gctgtcagga aatcataatc
    10501 agttccgtca ttattttgaa aatggtaagt taacctagga acttctgtat tatcctcttg
    10561 aacaattgca taaatggaga atgaatctgt tttaaaagca acatcactgc tagtgttctt
    10621 agtttctgca gtatctttag attttaatac ttctgtttga ccatcatctt taaagtgaac
    10681 aactttaagg ttttcatctg aagcttctaa tggcttatca tagttaacct ctacttttac
    10741 tggagcttgg ggttctgctt ctttaccatt tgactcaata gtaatgtcat agagtttgaa
    10801 atttttgatt tcactatctt gtttagcaac ctctgtcaat gcttttttct tataatcttt
    10861 aaaagtagct gaattgtctt ttaattccgt cacctttaaa tcagcatttt taggaatctt
    10921 agcttctttg gtcaaagtca ctgttacagt atagtctgca cctctaaaca tcaatggttc
    10981 ttcacgataa gcagcttcct cagaagatga tgtttctgtt acactagaag caggagtctg
    11041 gggcttgctc tgctcaacac ttgattgaga actagatgtt gatgaagtta cctggctaga
    11101 atttttattt tctaaagtaa tccccacatc atctgtctta gtttcttcaa ctgttattgc
    11161 tggtagaatt aaaaaataag tcgttaaaaa agttgttagg atcatcaatg accacatgat
    11221 aattttccac tctttagggt gttttttctt ttttaatgat tcgattataa aagtttgaca
    11281 cttctttagc atttttgcat cctccctaac cttaattgat actactaatc ttacctagag
    11341 gccatattct gaaagaaatt ttacctacaa tttgttcttc tgaaacatct cctacagaag
    11401 tacttcgaga atcaattgaa gtttttcggt tgtctcctaa tacaaaaatt tttttatcag
    11461 gtacttgata tgggtatttt atattactat taccgagtgc tttatgaata acatatggtt
    11521 ctttcaactt atgttgattc acgtaaacat ccccttgaga atcaatatta acccagtctc
    11581 ctgactctgc aataacccgc ttgactagga ccttattatt gtagtaaaac gcgacaacgt
    11641 ctccagtttt aaaatttgaa ccttttactg taaagactac atcacctgca cttaaagtct
    11701 tattcattga atgtccgtag attcttaata caggcaacca taaaaccgca attaaaatgg
    11761 ctattgaggc aaccgccatc aagatgtata tggtattttt aatgacactc caaaagcgtt
    11821 tctgataagt tacgcgatcc agttcttgag ataatttatc tgaactaatc tgtcttttca
    11881 ttgtctacgc ctctcttatc tactaaattc tttacgtttt ctaaataggt atctgcagct
    11941 aactgagcag tctcaaaaat accactaagc attaaggaag cttcggcaat agaaccagct
    12001 ttgctaattt tgattttttt atcatctagc gcttctttaa gttgctgaat ctctttctct
    12061 tgtttttcaa taagaagttg ctgttctaac ataatttcta gcaagtcttt tctttttaat
    12121 ttttttaaat cttccatcgc gattacttcc ttaactgaac cttaaattat cgtttagata
    12181 ttatatcaaa gttctaacct ttaaactcat tttttgtcct gtgttttttc tcaaaaaagt
    12241 ctatgctaaa ttaacatttt tgataatttt ttgaaaaatc tcatcgaagt cattttcttt
    12301 ttgaaagctc gaattctagg cattaaaaa
//
LOCUS       DBJYSH010000062        12061 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.062, whole genome shotgun sequence.
ACCESSION   DBJYSH010000062 DBJYSH010000000
VERSION     DBJYSH010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 12061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12061
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.062"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            complement(<1..903)
                     /locus_tag="ACUBV3_001298"
     CDS             complement(<1..903)
                     /locus_tag="ACUBV3_001298"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HHU3847802.1"
                     /translation="MPYDIPASWEWVRLRNISSLSFFPNISGDKIPNYSWVLDMEDIE
                     KETGRLVRKNYKTEKSSYKSNKVYFSKDTVLYAKLRPNLKKVIISDEDGFATTELIPI
                     KIFGGISAEYMRYCMISPSYYFNIIKSVYGVKMPRVNATFLNSTLLPLPPLSEQKRIV
                     EQIERALEKVDAYSESYNKLQELDKSFPDKLKKSILQYAMQGKLVPQDPNDEPVEVLL
                     EKIQAEKQKLYEEGKLKKKDLAEIVVTKGDDNSPYKEVPYNIPKSWEWVRLGNISSLN
                     FFSSISGDKIPNDSWVLDMEDIEKE"
     gene            1024..2457
                     /locus_tag="ACUBV3_001299"
     CDS             1024..2457
                     /locus_tag="ACUBV3_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HHU3847803.1"
                     /translation="MNQTLSHYQFSPETQYLDRKSARKKPSELLKHLIAFANADGGQL
                     VIGIEDDKQNNIITGFKDGRAYPIDDFKKIDREMRDTPLDLSFEEIPVRNHKGEDDVI
                     LVISVELSSNRVIAAPNDEVYLRQGDETVKLSYEQRTQLSYDKGQRFFEDEVVADATL
                     EDIDDDLVQDFKNRFDIADRSTEEILKARRFLVNGKLTKAAILLFGKYPSAFFPQARV
                     RFQRFDGTDMGTGSSFNVIKEVTFDDALPTLIIKARDFIRTQLREFQYLDDNGQFQIL
                     PEYPEFAWFEGVVNAVTHRDYSVYGDHIRVLMFDDRLEIHSPGKLPNIVTVDNIKHER
                     FSRNPRIARTLTEFGWVREMNEGVKRIYSEMESAFLHEPKYSEPGNKVILTLENNIVS
                     RHLRTQDSLEKQFTDFGDLNADEQLLIHYMYNSGEKMTTAKAIELTGRSRKFIVKTMK
                     KLQELGMVDWYGANKNDRNQYYTLTKN"
     gene            2594..4165
                     /locus_tag="ACUBV3_001300"
     CDS             2594..4165
                     /locus_tag="ACUBV3_001300"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF016724.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HHU3847804.1"
                     /translation="MEKFKQEIKNIGSNKLAYSIDLNNQKITKSEIVKSWGKAYSEKV
                     ANGSLGNGLRPPQYGALSAIRSHWVVSNEPATIVLPTGTGKSETIFSTIVSERIATTL
                     IVVPSNLLREQLFDQVKHLGILPKISVVSDQAIFPNCLLYKSTVKDSEKDILFNEIDN
                     INIIITTPALIKALPNDLFEKIYSNVELVVFDEAHHLAANDWRKVRDKFSGKKILQFT
                     ATPFRNDGKKIDGKIIFKYSLSLALQNNYFKPIDFHPIHEYNITKSDEKIADVAVKLL
                     QSDLDKGLNHILLARANSKKRADELFDKIYSNYKIYNPVIIHSDRKKSQNRDSLDLLK
                     SGKSQIVVCVDMFGEGIDIPSLKVAAIHDKYKSLPITLQFMGRFARSGKERLGSAKLI
                     TNIASEDLKEDIDELYRRDADWTKLLQIKSDRAIEKEVQIEEFISQFEKTRASNIDLS
                     QIKMKISSRIYKTDMKEVNINAWKNVLSAEHTTHLINEEKGILIFIEEIENTVNWSYQ
                     KDLVEYLYNFFVIFF"
     gene            4179..5621
                     /locus_tag="ACUBV3_001301"
     CDS             4179..5621
                     /locus_tag="ACUBV3_001301"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3847805.1"
                     /translation="MIHINESDSRKGNDLVEQIFPNSMQIKGDEIYRVLDGINRLMIG
                     TLGLKQIPGGNVSFRMFAGVDVRKGISEATSLGSIKSNIFGHGYRDGNKISVGCSYKG
                     KVWMRWVESLNFWVDWCQKIGAKILDTNIDTSMILENSLTSEVVTEFPDGVPYKITPD
                     SIIETSNSTARAFYIEKEDKLYHFFESDFRNPRLEKGLLIFELWISERKFIFQQHIDK
                     KGFGFLQISGDDLFIKRSNNKILFSQYLKDHSPTISFIQKDGVRVMLEGNLQIVDKPR
                     SKASLSNEMLVPIEWKKYGTNIRKESQGVAKDRASIQYVTINKLINTDELIVFDDDGS
                     GEIADIVTISANEAERKILINLYHCKYSHGDEPGARVSDLYEVCGQAEKSIIWKDNLL
                     DFLDRMIKRESIRISKRQSSRFEKGNINDCRTIKSMIKDGFSTDMRITIVQPGVSISK
                     LSTEMKQLLLSTEYYLSETYNIPLNCYFSE"
     gene            6390..7250
                     /locus_tag="ACUBV3_001302"
     CDS             6390..7250
                     /locus_tag="ACUBV3_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HHU3847806.1"
                     /translation="MFIYGPIFREFRLNRQFSLKQVASNELSVSQLSRFERGESDLSL
                     TKFLGALEAIDLSISEFMYRVNKYQKSDQISLMSQMAQYHYQRDVAGLEKMISVEEGK
                     LKKDSSDIRCRLNIVLFRGMICECDSSRKMSEEDLCFLSDYLFQKDSWEISDYILIGN
                     LYRYYNTRYICQLVKEVINQKEYYRDIYTNRNVVEATLLNVVETLIERRALEEATFFV
                     EKVEALLNNERNAYHRIILLYEKGFLAYAKGDSRGIQSMKQAIFCFQAIGSKHHVENF
                     QEHFNRVTRL"
     gene            7323..8503
                     /locus_tag="ACUBV3_001303"
                     /pseudo
     CDS             7323..8503
                     /locus_tag="ACUBV3_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024508.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(8565..9215)
                     /locus_tag="ACUBV3_001304"
     CDS             complement(8565..9215)
                     /locus_tag="ACUBV3_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HHU3847807.1"
                     /translation="MKQMNTFQQFIYYFQHNGSYILEQFIHHFLISIYGVILAAIVGI
                     PLGIFIARRGKIKTFVMGIANVIQTIPSLAMISIIMLGLGLGIKTVVATVFLYSLLPI
                     ITNTYTGIRNVDSDLLDAAKGMGMTKRQRLFMVELPLSISVIMAGLRNALVVAIGITA
                     IGAFVGGGGLGDIIIRGTNATNGGAIILAGSLPTALMAIFSDLILGGIQRMLEPRK"
     gene            complement(9216..10028)
                     /locus_tag="ACUBV3_001305"
                     /pseudo
     CDS             complement(9216..10028)
                     /locus_tag="ACUBV3_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003562594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
     gene            complement(10145..10780)
                     /locus_tag="ACUBV3_001306"
     CDS             complement(10145..10780)
                     /locus_tag="ACUBV3_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000247049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HHU3847808.1"
                     /translation="MVNFLSQYGMQILVKTWEQVYISFFAIALGIAIAVPLGVVLTRF
                     PKVAKIIIAIASMLQTIPSLALLALMIPLFGIGKVPAIVALFIYSLLPILRNTYIGMN
                     NVNPTLKDCAKGMGMKPIQSIFQVELPLATPIIMAGIRLSTIYVIAWATLASYIGAGG
                     LGDLIFSGLNLFQPKLILGGTIPVIILSLIIDYLLGLLETALTPRTTRREA"
     gene            complement(10780..11925)
                     /locus_tag="ACUBV3_001307"
     CDS             complement(10780..11925)
                     /locus_tag="ACUBV3_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529591.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HHU3847809.1"
                     /translation="MGQEPIIEYQNINKVYGENVAVEDINLKIYPGDFVCFIGTSGSG
                     KTTLMRMVNHMLKPTNGTLLFKGKDISTINPIELRRRIGYVIQNIGLMPHMTIYENIV
                     LVPKLLKWSEEAKRAKARELIKLVELPEEYLDRYPSELSGGQQQRIGVIRALAADQDI
                     ILMDEPFGALDPITREGIQDLVKSLQEEMGKTIILVTHDMDEALKLATKIIVMDNGKM
                     VQEGTPNDLLHHPATSFVEQMIGEERLLHAQADITPVKQIMLNNPVSITAEKTLTEAI
                     TLMRQKRVDSLLVTDNGKLIGFIDLESLSSKYKKDRLVSDILKHTDFYVMEDDLLRNT
                     AERILKRGLKYAPVVDHENNLKGIVTRASLVDMLYDIIWGDTETEDQ"
BASE COUNT         4006 a         1890 c         2238 g         3927 t
ORIGIN      
        1 ctctttttct atatcttcca tatctaaaac ccagctatca tttggaatct tatctccaga
       61 tatactagaa aaaaaattca acgaagatat attacctaac cttacccatt cccagctttt
      121 tggaatgttg tacggcactt ccttataagg agagttatca tctccttttg tcaccactat
      181 ttctgccaaa tctttcttct tgagtttacc ttcctcatag agtttttgct tttcagcttg
      241 gattttttca agcaaaacct caacgggttc atcgttaggg tcttgaggaa ccaacttacc
      301 ttgcatcgca tattgaagaa tagatttttt caacttatct ggaaaggatt tgtccaattc
      361 ttgaagttta ttgtaacttt cggaatatgc atctaccttt tccaaggcac gttcgatttg
      421 ctccacaatc cgtttctgtt cagagagagg cgggaggggg aggagagttg aatttagaaa
      481 cgtagcattt actcttggca ttttaacacc ataaaccgat ttgatgatgt tgaaataata
      541 agatggagaa atcatacagt atctcatata ctctgctgaa atacccccaa aaattttaat
      601 cggaataagt tcggttgttg caaaaccatc ttcgtcagaa atgataactt tctttaggtt
      661 ggggcgtagc ttagcatata aaacagtatc cttagaaaaa tatactttat tgcttttata
      721 agatgatttt tctgttttat agttttttcg aactaatctt ccagtttctt tttctatatc
      781 ttccatatct aaaacccaac tataattcgg aattttatct ccagatatat tagggaaaaa
      841 actcaacgaa gatatatttc gcaacctcac ccattcccaa ctagctggaa tatcataggg
      901 cacttccact tccttaatcg tcccgtctga taacttctca taaggtttct tagcacaatg
      961 aattggaagc aagaaagaat gggagatgtg ttataataga gctataacac tcaggagaat
     1021 gacatgaatc aaaccctatc ccactaccag ttctcaccag agactcaata cttggacagg
     1081 aaatctgcac gtaaaaagcc gtctgaactt cttaaacacc tgattgcctt tgctaatgca
     1141 gatggtggcc agctggtaat cggtattgag gacgacaagc aaaataacat cattactggc
     1201 ttcaaagatg gcagagccta tcccattgat gacttcaaaa agattgaccg tgagatgcgt
     1261 gatactcctt tggacttatc ttttgaagaa atccctgtgc gaaaccataa gggtgaagac
     1321 gatgtgattt tggttatctc agttgaatta tcttcaaatc gagtgattgc tgcgcctaat
     1381 gatgaagttt acctgcgtca aggggatgaa acggtaaaat taagttatga acaacgaact
     1441 cagctcagct atgataaagg acaacgtttc tttgaggatg aagttgtggc tgatgcgacc
     1501 ttggaagaca tcgatgatga cctggttcag gacttcaaaa atcgctttga tattgcagac
     1561 cgttccaccg aggaaattct caaagctcgg cgttttctgg tcaatggaaa attgaccaag
     1621 gctgctatct tgctttttgg caaataccct tcagctttct tccctcaagc tcgtgttcgc
     1681 ttccaacggt ttgatggcac ggacatgggg acaggtagta gctttaatgt cattaaggaa
     1741 gtgacctttg atgatgctct accaaccctg attatcaagg ctcgtgattt tattcgtacc
     1801 cagctgcgtg agttccaata tctggatgac aatgggcaat ttcaaatttt gcctgaatat
     1861 ccagaatttg cctggtttga aggtgttgtc aatgctgtta ctcaccgtga ttactctgtc
     1921 tatggcgacc atattcgtgt tcttatgttt gatgaccgac tggaaattca tagcccaggg
     1981 aaattgccaa atatcgtcac cgttgacaat attaagcatg aacggttttc aagaaatcct
     2041 cggattgcca gaaccctgac ggagtttgga tgggtccgtg agatgaatga aggtgtgaaa
     2101 cggatttact cagagatgga atcagccttt ctccatgaac caaaatactc tgaacctggg
     2161 aacaaggtta ttctgacgct tgaaaataat attgtcagcc gacacctacg gacccaagat
     2221 agtttggaga aacagtttac tgattttgga gacctcaatg ccgatgagca attgttgatc
     2281 cattacatgt acaattcagg agagaagatg acaactgcaa aagccattga actgacagga
     2341 agaagtcgta agtttattgt aaaaacgatg aaaaaattac aagaattggg tatggttgac
     2401 tggtatggtg ccaataaaaa tgacagaaat caatactata ctttaacgaa aaattagcct
     2461 atcctctaag agataggata cagtaggata cagtaggata cagtaggata cagtaggata
     2521 cagtaggata cagtaggata cagtaggata cagtaggata cagtaggata cagtaggata
     2581 cagaggcttt gtgatggaaa aattcaaaca agaaattaag aatattggtt caaataagtt
     2641 agcctattca atagacctta ataatcaaaa aattaccaaa tcagagattg taaaaagctg
     2701 gggcaaagcg tattctgaga aagttgctaa cggttcttta ggtaatggat tgcgccctcc
     2761 tcaatatggt gcactttctg ctatacgttc tcattgggtt gtctctaatg aaccggccac
     2821 aatagtttta ccgacaggaa caggaaaatc tgaaacaatt ttttcgacaa tagtctcaga
     2881 gcgtattgct acaactttga ttgttgttcc aagtaattta cttagagaac aactttttga
     2941 tcaggttaaa catctgggga ttcttcctaa aatttcagtt gtatctgatc aggcaatttt
     3001 tcctaattgt ctattatata aatctactgt gaaagatagt gaaaaggata ttttatttaa
     3061 tgaaattgat aatataaata tcattataac tacaccagcg ctgattaaag cgttaccgaa
     3121 tgatctgttt gaaaaaatct attcgaacgt tgaattagtt gtatttgatg aagctcacca
     3181 tcttgctgca aatgattgga gaaaagtaag agataagttc tcaggtaaaa agattttaca
     3241 atttacagcg accccatttc gaaatgatgg taagaaaatt gatggaaaaa taatttttaa
     3301 atatagttta tctcttgctc tgcaaaacaa ttattttaaa ccaattgatt ttcatcctat
     3361 ccatgagtat aatattacaa aatcggatga aaaaatcgca gatgttgcag ttaaactttt
     3421 gcaaagtgat ttggataagg gattaaatca cattcttttg gcgagagcta actctaaaaa
     3481 aagagctgat gagttattcg ataaaatata ttcgaattac aaaatatata atccagtgat
     3541 tatacattct gatagaaaaa agtcgcaaaa cagagattcg ttagatctac taaaatcagg
     3601 aaaatcacaa attgttgtat gcgtggacat gtttggtgaa ggtattgata ttccttcact
     3661 taaagttgct gcaattcacg acaaatataa gtcattacca attactttac agtttatggg
     3721 aaggtttgca aggtctggta aagaaaggtt aggcagtgct aaactgatta ctaatatagc
     3781 atcagaagac ttaaaagaag atatcgatga attgtataga cgtgatgcgg attggactaa
     3841 actgcttcaa attaagtcag atagagcaat agaaaaagaa gttcagatag aagaatttat
     3901 ttcccaattt gaaaaaacaa gagcaagtaa tattgactta tctcaaataa agatgaaaat
     3961 tagttctcga atatataaaa cggatatgaa agaggtaaat atcaacgcct ggaaaaatgt
     4021 tctgagtgca gaacatacta ctcacttgat aaatgaagag aagggaattt taatatttat
     4081 tgaagaaata gagaatacag ttaactggtc ttatcaaaaa gatcttgtcg aatatttata
     4141 taattttttt gtaatctttt tttgatgagt atagaggttt gattcatatt aatgagtcag
     4201 atagtagaaa aggaaatgat ttggtagagc aaatttttcc taattctatg caaatcaagg
     4261 gagatgagat ttacagagtg cttgacggta ttaatcgact aatgattgga acacttggtt
     4321 taaaacaaat acctggagga aatgtcagtt ttcggatgtt tgctggggtt gatgtaagaa
     4381 agggaataag tgaagcaact tcattgggtt caatcaaatc aaatattttt ggacatggat
     4441 acagagatgg aaataaaata agtgtaggtt gttcttataa aggaaaagtt tggatgaggt
     4501 gggttgaaag tcttaatttt tgggtagatt ggtgccaaaa aattggggca aaaattttag
     4561 atactaatat tgatacaagt atgatattag aaaactcact tacttcagaa gttgttacag
     4621 aatttccgga tggggttcct tataaaataa cacctgacag tataattgaa actagtaatt
     4681 caactgcaag ggcattctat attgaaaaag aagataagct gtatcatttt tttgaatcag
     4741 actttaggaa ccctcgtctt gaaaaaggct tattgatttt tgaactttgg attagtgaaa
     4801 gaaaatttat tttccaacaa catatagata aaaaggggtt tggttttttg caaatttctg
     4861 gagatgactt atttataaag agatcaaata ataagatttt attttcacaa tatttaaaag
     4921 atcatagtcc aactatttct ttcatacaga aggatggggt tcgggtaatg ttagagggta
     4981 atctacaaat tgtagataaa ccgaggagta aggcatcgtt gtcaaatgag atgcttgttc
     5041 cgatagaatg gaaaaaatat ggaacaaata tacgaaaaga gtctcaaggt gttgctaagg
     5101 atagagcatc cattcaatat gtaactataa ataaattgat aaatactgat gaattgatag
     5161 tttttgacga tgatggttca ggagaaattg cagacattgt tacaataagt gctaatgaag
     5221 cagagcgcaa aatactcata aatctgtatc attgtaaata ttcacatggt gatgaaccag
     5281 gtgctcgtgt atcagattta tacgaagtgt gtggtcaagc agagaaatca attatttgga
     5341 aagataattt attagatttc cttgatagaa tgataaaaag agagtcaata cggatttcta
     5401 aaagacagag ttctcggttt gaaaaaggaa atattaatga ttgtcggaca atcaaatcta
     5461 tgataaaaga tggctttagt actgatatga gaataaccat tgtacagcca ggtgtgtcga
     5521 tttcgaaact aagtactgag atgaagcaat tgttattatc aactgagtat tatttaagtg
     5581 agacatataa tattcctctt aactgttatt tcagtgaatg agaaatagtt ttaaaattcg
     5641 aatactctat gctttttatg agtatgaatc ttgagtgata taagtcacta gcaagttaat
     5701 ataaaaaaag ttaattatac aatgattttc ctgttcattt actcaattac ataagctccg
     5761 aaaacgaatt tttggtaatc taatggtaat ccattgtttg taggattagg ttataagtgc
     5821 ttatttttaa tgaaactgac ttttgaaagc ttgatttaat aagcttttat tgaggtttta
     5881 cttttgctac ccaaaaccag gtttaaaaaa atcccgtaaa gcatcacaat cctcaaaacg
     5941 ttaatatata cctatatatc agcgattgga agcacttcat ggttcacacc atggggtgtt
     6001 ttttgtgtaa tattcaaaaa ttgtcctaaa gtcattttta aaacttctaa atacttgaga
     6061 aaccattgta aatttaaata actactttaa atagcacaaa ggcaatgaca aagttagatg
     6121 agatgattct attttaattt ataattgaaa aaaagagtat atgatggagg aattttgtat
     6181 ggtaagtact caatatatga aagtaacctt acgagaaaaa ttcccagata aattaacata
     6241 tgttgattta gaagaacttg tgatatgata ttacaaataa aagaaaatga ctatatatca
     6301 gctcatatat tggttaaagt tggtaaatat aaattattga aattacctca taagtttacc
     6361 aatgagatta cggttagaaa ggaatactaa tgtttatata tggccctatt tttagggaat
     6421 ttcgacttaa tcgacagttt tctcttaaac aggttgcttc aaatgaactt tctgtttctc
     6481 agttatcacg atttgagagg ggtgaatcag atttatcatt aacaaaattt ttaggggctt
     6541 tagaggcaat tgatttatca attagcgaat ttatgtatag agtaaataaa tatcaaaaat
     6601 ctgatcagat atctttaatg tctcagatgg ctcaatatca ttatcaaaga gacgtagctg
     6661 ggttagaaaa gatgatttct gtggaagaag gtaaactaaa aaaggatagt tcagatatac
     6721 gttgtcgttt aaacatagtt ctttttagag gaatgatatg tgaatgtgac tcctcgagaa
     6781 aaatgtcgga agaagacctt tgctttttgt cggattacct ctttcaaaaa gatagttggg
     6841 aaattagtga ctatattttg attggaaatc tttaccggta ttacaacaca aggtatattt
     6901 gccaacttgt taaagaggtt atcaatcaaa aggaatatta tcgagatatt tatacaaacc
     6961 gcaatgttgt cgaagcaacc ctcctaaatg tggtagagac actaatagaa cggcgcgctc
     7021 tagaagaagc aacatttttt gtagagaaag ttgaagcact tttaaataat gagcggaatg
     7081 cttatcaccg tattatttta ctctatgaaa aaggatttct tgcctatgct aaaggggata
     7141 gtcgaggaat acaaagcatg aagcaagcta ttttttgctt ccaagcaatt ggttctaaac
     7201 atcatgttga aaattttcag gaacacttta atagggtaac aagattataa aaaatcccat
     7261 ttatgggatt ttttaatatt tctccttttg gtttgctata atgtcgaact aaggagggaa
     7321 ttatgaatat taatggtata aaattactat ctagtcgagc agtcagtaaa cttggtgatg
     7381 tgctttatga ttatggaaat agtacttgga ttgcttcgat gggtggtcta ggacaaaaga
     7441 ttttaggtat ttatcagata gtagagttgt tggtttctat tgtcctcaac ccttttggag
     7501 gcgccttagc tgatcgcttt caacgaagaa aaattttact aataacagat gctatctgtg
     7561 ctatcatgtg tttcttactt tcttttattg gagatgataa agtaatggtg tatggtctaa
     7621 tagttgctaa tgctatctta gcggtttcca atgctttttc ttctccggct tataaatctt
     7681 atattcctga aatagtagat aaagctgata tcattaccta taatgctaat ctggaaacca
     7741 ttgtgcaaat tattagtgta agttcccctg ttttaggttt ccttattttt aataattttg
     7801 gtattaggat tactttaatt gttgatgcga ttactttttt gatctctttc ctgtttcttt
     7861 atgctattaa agtggaaggg tacagctttc aaaacaagaa aaggtagcga taaaaaatat
     7921 tttagctgat attgcagatg gatttactta catcaaaaaa gaaaaagaaa ttatgttttt
     7981 tctaattatt gctgctttat taaacacttt cctagcaatg ttcaattatc ttttaccttt
     8041 tactaactcc ctattgaaaa cttcaggtgc ttatgcaaca atacttagta tatcagctat
     8101 cggatcaatt attggagcgc ttattgctag aaaaattaaa tctagtatta attctatgct
     8161 atctatgcta gtattcagta gtttgggagt catagttatg ggattcccat ctctatttga
     8221 attaccgata tggatatctt acagtggtag ttttcttttt aatagtcttc tgacaatgtt
     8281 taatattcat tttttcagtc aggtataaat cagggtcgat gaagcgtata tggggcgtgt
     8341 gatgtcgact atatttacta ttgctattat gtttatgcct ataggaacac tatttatgac
     8401 tatcttttca tttgccttat caaatgttag ctttattgtt ataggatgtg ccattgctat
     8461 attgggagga ttaggattta gctatagtaa aaaacaattt tagaacttaa gaaattattc
     8521 tgaaacatca aaaaaacttg tctctactga caagttttta atagttattt tcttggttca
     8581 agcatgcgtt ggataccgcc taggattaaa tcagagaaaa ttgccataag ggctgttgga
     8641 agagatcctg ctaagataat agcaccacca tttgttgcat tagttcctct tatgatgata
     8701 tctccaagtc cgcctccacc gacgaaagca ccaatggctg taataccaat tgctacaaca
     8761 agggcatttc taagtccagc catgataaca gaaatagata gtggtaactc aaccataaat
     8821 aaacgttgac gcttagtcat gcccattcct ttagcagcat ctagtaagtc ggagtcaaca
     8881 tttctaatac cagtgtaagt atttgtgatg attggcagaa gagaataaag aaagacagtt
     8941 gcaactactg ttttaatacc tagccctaga cctaacataa tgatagagat catagctaaa
     9001 gaaggaatgg tttggatgac atttgcgatt cccataacaa aggttttaat tttaccacgt
     9061 ctagcgatga agatacctag agggatacca acaattgcag ctaaaataac gccgtaaatt
     9121 gaaattaaaa agtggtggat aaattgttct aagatataac taccattatg ttggaagtag
     9181 tagataaatt gttggaaagt attcatttgt ttcatttatt tatctcctct aaaataatgg
     9241 tttttttcta aaaattgttt ggcaacaact gaaggttcta aaagtttatc atctaccata
     9301 taattaaggt tttgcatcgt ttttaaattg attttaccat cgagtcgatg gagtaatttt
     9361 tttagtttag gatctttttt gatgatagaa ttgttgacaa ccatagaggc ttcataagga
     9421 ggaaagaatt ttttatcatc ccttaaaatt tctaaatcat agctcgaaat acgaccgtca
     9481 gtggagtagc ctaatacaga ttgcattttg ttactttcaa ctgcatcata gactaagcca
     9541 atttgcatag ggtaaatatg tgaaaattca aaaccgtatg ttttagcgaa atcagtgtat
     9601 ccatctccct cgcgattcat ccatgaacta tcaacccctg ccttcatagt tgttgataac
     9661 tttttgagat cagagatctt ggtgatttta ttctgtctgg caaactcttt agtaaccatg
     9721 aatgcataag tatcagaaaa accataagta ggataccaag tttgattgta gcgtttttgg
     9781 aattcagttt ttacaatctt agaagcttct ttagggtctt taacagcttt taagccaaga
     9841 gttcctgtga tgtctgttcc tgtataacgt gtggcagcaa tgtcagcatc accacggagc
     9901 aaagcttggt gagtaaccgt agaggaacca agattgctta ttaaagttgt gttgtatcct
     9961 aattcgtgat gaattaattc ggtgacaata tttgccatga tactagactc tgtagaactt
    10021 tgggcagcaa ccttaattgt ggtatgacca ggttttttag tatcggttaa ttgacaacca
    10081 gaaatcgtta agagggctaa gcaaagtgta aatatctgta aaaagtgcga ttttttaagc
    10141 atatctaagc ctcccttctt gttgttctgg gcgttagagc tgtttccagt aatcctaaaa
    10201 gataatctat aataagtgac aggataataa caggaattgt gccccctaga attaatttgg
    10261 gctgaaaaag atttaacccg ctaaaaatga gatcaccaag tccacctgca cctatataag
    10321 aagctaatgt agcccaagca ataacataaa tagtagacaa gcgaatacca gccataataa
    10381 ttggagtagc taaaggtaac tcaacttgaa atatagattg aataggtttc attcccatcc
    10441 ctttagcaca atctttaagt gttggattga cattattcat acctatataa gtattcctta
    10501 gtataggaag caaggaataa ataaataaag caacgatagc tgggactttt ccaattccaa
    10561 acaatggaat cattaaagct aataaggcta aacttggtat agtttgtagc atgctagcta
    10621 tagcaatgat aattttggct actttgggaa aacgtgttaa gaccacaccc aatggaacag
    10681 caattgcaat cccaagtgct atagcaaaaa atgagatata gacttgttcc catgttttta
    10741 ctaatatttg catgccatac tgtgataaga aattgaccat tattgatcct ccgtttcagt
    10801 atcgccccaa ataatatcgt ataacatatc aactagggat gcacgagtaa caatgccctt
    10861 taggttattc tcatggtcaa caactggagc gtattttaaa ccacgtttta aaatacgctc
    10921 agcagtattt ctaagtaagt cgtcttccat aacataaaaa tcagtatgtt ttaagatatc
    10981 agaaacaagt cggtctttct tatatttact gcttagagat tctaagtcaa taaaaccaat
    11041 taatttaccg ttatcggtta ctagaagtga gtcaacgcgt ttttggcgca ttagtgtaat
    11101 agcttcagtt agtgtttttt cagcagttat tgaaacagga ttatttaaca ttatctgttt
    11161 aacaggggta atatcagcct gcgcatgaag aagacgctct tccccaatca tttgttcaac
    11221 gaaactggta gcaggatgat gtaagagatc attgggtgtc ccttcttgga ccattttacc
    11281 attgtccata acaataattt ttgttgctaa cttgagggct tcatccatat catgagtaac
    11341 taagatgata gttttcccca tttcttcctg aagagacttg actaagtctt gaataccttc
    11401 tctagtaata ggatccagag ctccaaaagg ctcatccatt aaaataatat cttggtctgc
    11461 tgcaagagcg cgaatgacac cgatacgttg ttgctgaccg ccagacaact cactagggta
    11521 gcgatccaaa tattcttcgg gtaattcaac taatttaata agttcccttg ctttagctct
    11581 tttagcttct tctgaccatt tcaataattt tggtacaaga actatatttt cgtaaatggt
    11641 catatgaggc attaaaccaa tgttttggat aacatatcca attctgcgtc ttaattcaat
    11701 ggggttaata gtagaaatat cttttccctt aaataataga gtaccatttg ttggttttaa
    11761 catatggtta accatacgca ttaatgttgt tttacctgat ccactcgtac cgatgaaaca
    11821 aacgaaatca ccagggtaaa ttttaaggtt aatatcttca accgcaacat tttccccata
    11881 cactttattg atattttgat attcgatgat aggttcttgg cccatattat tctcctctac
    11941 aaactatatt atagtaataa tatcaaaact ttggcaacat gtctgaaatt tgttttaaaa
    12001 tttaatgatt ttaaaaagaa tcgttaagct tgcgcccgat ctacattttc agaaaatgta
    12061 g
//
LOCUS       DBJYSH010000063        11995 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.063, whole genome shotgun sequence.
ACCESSION   DBJYSH010000063 DBJYSH010000000
VERSION     DBJYSH010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 11995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11995
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.063"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            complement(126..2777)
                     /gene="essC"
                     /locus_tag="ACUBV3_001308"
                     /pseudo
     CDS             complement(126..2777)
                     /gene="essC"
                     /locus_tag="ACUBV3_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000960154.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssC"
     gene            complement(2762..3388)
                     /locus_tag="ACUBV3_001309"
                     /pseudo
     CDS             complement(2762..3388)
                     /locus_tag="ACUBV3_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000826106.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
     gene            complement(3402..4325)
                     /locus_tag="ACUBV3_001310"
     CDS             complement(3402..4325)
                     /locus_tag="ACUBV3_001310"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001016473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HHU3847810.1"
                     /translation="MNHQTQTLSLEHFVSLEELSNQEVMSLIKRSIEVKENPSNIGFD
                     KDYYVSNLFFENSTRTHKSFEMAELKLGLKTIEFNADTSSVNKGETLYDTILTMSALG
                     LDVCVIRHPDIDYYKELIASPNIHSAIVNGGDGSGQHPSQSLLDLVTIYEEFGYFKGL
                     KIAIVGDLTHSRVAKSNMQVLKRLGAEIFFSGSKEWYSSQFDEYGQCLPIDQLVDQID
                     VLMLLRVQHERHDGKGVFSKESYHQQFGLTKERYKHLRDTAIIMHPAPVNRDVEIASD
                     LVEADKARIVKQMSNGVYARIAILEAVLNSR"
     gene            complement(4490..5782)
                     /locus_tag="ACUBV3_001311"
     CDS             complement(4490..5782)
                     /locus_tag="ACUBV3_001311"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HHU3847811.1"
                     /translation="MYIIKNGLIIDPQSGFNQVSDMLIDQGKIKQISKEIDIKGIPII
                     DASNKIVAPGLVDIHVHFREPGQTHKENIHTGALSAAAGGFTTVLMMANTNPTISSSE
                     IVKQVKESAAKEAIKIETVATITKSLNGKDLVNFEELLEAGVAGFSDDGIPLTDTKVL
                     QEAMNLARKHDVVLSLHEEDPSLNGVLGINEHIAQKIYHVCGASGLAEYSMIARDAMI
                     AYQTQAKVHIQHLSSSESVEVVDFAQKLGANLTAEVTPQHFSKTEDLLLTKGANAKLN
                     PPLRLEKDRQALIDGLKSGVISIIASDHAPHHIMEKAADNISQAPSGMTGLETSLALG
                     ITYLVSTKELSMIDFLAKMTCNPAQLYGFDAGYLREGGPADIVIFDQAEERIIKAEFA
                     SKSSNSPFIGDKLKGVVHYTICNGEIVYQKDSHAATLV"
     gene            complement(5794..6423)
                     /gene="pyrE"
                     /locus_tag="ACUBV3_001312"
     CDS             complement(5794..6423)
                     /gene="pyrE"
                     /locus_tag="ACUBV3_001312"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HHU3847812.1"
                     /translation="MDLARQIAMELLDIQAVYLRPQQPFTWASGVKSPIYTDNRVTLS
                     YPETRTLIENGFVKQIQKHFPNVDIIAGTATAGIPHGAIIADKMNLPFAYIRSKAKDH
                     GVGNQIEGRVYSGQKMVIIEDLISTGGSVLEAVTAAQSQGIEVLGVVAIFTYQLAKAE
                     QAFREADIPLVTLTDYNQLIKVAKVNGYITADQLVLLKKFKEDQMNWQS"
     gene            complement(6436..7137)
                     /gene="pyrF"
                     /locus_tag="ACUBV3_001313"
     CDS             complement(6436..7137)
                     /gene="pyrF"
                     /locus_tag="ACUBV3_001313"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HHU3847813.1"
                     /translation="MLEKCPIIALDFSDLASVTTFLEHFPKEELLFVKIGMELYYSEG
                     PSIIRYIKSLGHRIFLDLKLHDIPNTVRLSMSVLAKLGIDMTNVHAAGGVEMMKAARE
                     GLGEGPILLAVTQLTSTSQEQMQVDQHINLSVVDSVCHYAQKAQEAGLDGVVASAQEV
                     KQIKKQTNEHFICLTPGIRPPQTNQLDDQKRTMTPEQARIVGADYIVVGRPITKAENP
                     YQAYLDIKEEWNRIK"
     gene            complement(7350..8582)
                     /locus_tag="ACUBV3_001314"
     CDS             complement(7350..8582)
                     /locus_tag="ACUBV3_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001178658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine ThrE exporter family protein"
                     /protein_id="HHU3847814.1"
                     /translation="MQNDMSVVLRAGKLLIESGAEVYRVEDTMKHFAKALQIENFEAY
                     VVSSSIIASGINRYGKQEAKVCNTDGVTANLGRLEAVNNLSRQIAKQDLVSPEEIVKQ
                     LDLIEHQKDYSLLVTLISYFCGAGSFSLALGSSLLDSFSAAVTGLILGYFLNLMESRI
                     HTGFLLTILGSSVVALSANLLYFSGLGEHRSIIILGALMVMVPGAAFVNSVREFSQNN
                     FSTGLALIMSALLICISISAGVAITIEIIPFAEQMTGSFSGVPNTILEIIIRTLMAGL
                     GTIAFSILYHVPKRYFLDLGILGAISWMLYLILWQQFHMDAIAVFFPGLFITYFSRLL
                     AAKRKCPATVFLATSMFPLIPGLSFYRAVYFLLTGADALAMEYFRSCFVTAFTIAIAI
                     SIVQQIPLSFFIRRKMIK"
     gene            complement(8622..10163)
                     /locus_tag="ACUBV3_001315"
     CDS             complement(8622..10163)
                     /locus_tag="ACUBV3_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000025412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HHU3847815.1"
                     /translation="MSILEVKNLSHGFGDRAIFENVSFRLLKGEHIGLIGANGEGKST
                     FMSIVTGHLQPDEGKIEWSKYVTAGYLDQHTVLEEGQTVRDVLRTAFDELFKTEARIN
                     DIYISMADDGADVDSLMEEVGELQDRLESRDFYTLDAKIDEVARALGVMDFGMDSDVT
                     ELSGGQRTKVLLAKLLLEKPDILLLDEPTNYLDAEHIDWLKRYLQNYENAFVLISHDI
                     PFLNDVINIVYHVENQDLVRYSGDYTNFESVYAMKKAQLEAAYERQQKEIADLQDFVN
                     RNKARVATRNMAMSRQKKLDKMDIIELQAEKPKPSFEFKESRTPGRFIFQAKDLQIGY
                     DRALTKPLNLTFERNQKIAIVGANGIGKTTLLKSLLGIIPPISGNVERGDFIDLGYFE
                     QEVPGGNRQTPLEAVWDAFPALNQAEVRAALARCGLTSKHIESQIQVLSGGEQSKVRF
                     CLLMNRENNVLVLDEPTNHLDVDAKDELKRALKAYKGSILMVCHEPDFYEGWMDDVWD
                     FNQLS"
     gene            complement(10294..10632)
                     /locus_tag="ACUBV3_001316"
     CDS             complement(10294..10632)
                     /locus_tag="ACUBV3_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001165748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP cone domain-containing protein"
                     /protein_id="HHU3847816.1"
                     /translation="MQIIKRDGQIVEFDPEKIYQAILKAAQTVYVLDESLRQDLAQVT
                     KKVILDLEDAQVERPTINMIQSLVENRLLDAGYIHIAEHYISYRLQRDLERNGYDDQI
                     TVHLHFEKIK"
     gene            complement(10802..11878)
                     /locus_tag="ACUBV3_001317"
     CDS             complement(10802..11878)
                     /locus_tag="ACUBV3_001317"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HHU3847817.1"
                     /translation="MGYTVAIVGATGAVGTQMIRQLEQSNLPIEQVKLLSSSRSAGKI
                     LHFKDEAIRVEETTKESFYDVDIALFSAGGSISAKFAPYAVKSGAVVVDNTSYFRQNP
                     DVPLVVPEVNAHAMIGHNGIIACPNCSTIQMMIALEPIRQKWGIERVIVSTYQAVSGS
                     GARAVEETKEQLRQVLNDNLSPDQLIATVLPCSSDQKHYPIAFNALPQIDIFTDNDYT
                     YEEMKMTLETKKIMEDATIKVSATCVRIPVLSGHSESIYIETKELASISEIKKAIANF
                     PGAVLQDLPSQQIYPQAINAVGHRETFVGRIRKDLDQENGVHMWVVSDNLLKGAAWNS
                     VQIAETLHKNGLVKPAKELKFELI"
BASE COUNT         3657 a         2361 c         2027 g         3950 t
ORIGIN      
        1 tctgttctgc ctggtattcc tttttcgtgc ggttttctgg gatttttgaa tccttacatg
       61 ctgtcatcat tatcattatt aataagcctc caaaaagcag taatagtctt tgttttttca
      121 tatgctcaat gctccatttc tatttcttga gtgattttaa gcattgtctc ttgtcttcta
      181 tcatgaatat aaatcatatc tggtttcaaa taaggttctt ttgaattata tgttttcggt
      241 aataccgact gatccataac tctcattccc aagagaacat actgtgcctg tgttctaata
      301 gcttttggaa catctgagat attagtcata aggtatgatg ctaagccacc aaataagaaa
      361 taaatatgat atttgtgagc cgagtcaagt aagaataaaa tctgttcttt atccatattt
      421 gtttctgcca caaaactatt catatcagga ataataacaa accatggttc tattttacca
      481 gattcttctc ttaaatagat ttctgaaaca atagcttcta aatgtcctgc tatttcactt
      541 cgattattga tatattcaat ttgttgttct gtcaaatcat cataatcaga agcagtgtct
      601 aaaagataaa ccttactatc tttaatcaat gaaacagatt taactaagaa gcgcgtcaag
      661 ttagacaaat tatctggatt gtcagaagta aaaagtaact gtttgaattt cgaaagtgga
      721 atgctacaat tatcaacttc ttttaagcta ataccaagtg aaatctgatt agtctcactt
      781 gcttttttaa catcctcata tgccttaaac tcctcaaagg taagtgtttc tggaacaatt
      841 ggtattgctt ttggtcttgg tccagtccat ttgctgttca tggcgtcaga ttcgttttgg
      901 atgttattga tgatgtcaaa ggtttcagtg ccttctgttg ggagagccac ttgtaacact
      961 tcaatgtcat cacgtttgat taatccgcgt cctggaatat cttccatgat gtgttggtga
     1021 cgaccgacaa gtatccgtga ttcagagtca tctgttaatt taagagcgat acgttcttta
     1081 aggtttgcca ttaaggacga cctcaaagct gccatacgac cagctgagat aactaaggaa
     1141 atgccaagtg acgacccatc acgcgaaata ttttggaaac aagcttctaa gttagtcagt
     1201 gtttgagctt cccgtagccc ttcataactg tcaataacaa tcaggatttg aggcatggtt
     1261 tccccagata cctggcggta aagttttgct gttgcaacat tgtagcgact gagggctttt
     1321 ttacggtcag acatttccgt tttgattctg ctgataaact tggctatttt ttcgtcatca
     1381 tcaatggtaa agaaatctgc tacatgtggc aaacgtctta atggtaagag gccattggta
     1441 ccaaaatcaa agagataaag gtttaaaaat tctggactat tgtgacgtgc taagtccaat
     1501 gttacagttt gaagggctgt ggatttcccc atacttggac ctgcaaaaat tgacaaatgc
     1561 ccatcatctt caaaatcgtg gtaagctact tcttgtgctt gttggcttgg tatatccata
     1621 aagcccagaa caactttgta agatggggct ttactccaga gaactactga ttcttcctta
     1681 cggagatcgg ttactgcaat ctgattgctt agaggcggta gccacagttg aggtaagtct
     1741 gagatgccca tttctttggt taaagtttga atgttttcaa cgatggcatc taattccgta
     1801 ggtacttctt taatattttc ggcttggtct aagcctgaaa gatcatcatt taggatttca
     1861 tattggccaa ggtcgttgat ggaataaatg gtatggtctt caatcccttg gtcgtctttt
     1921 tcaggttggt aatctgctcc tgaccatgct gattggaaga gttcatagac ttcattatta
     1981 ccaacttgga ggtaggcacg tcctgcttgt gtaatctcgg cggcatctgg ggtatggagc
     2041 atttccatgg agtcgccgcg gtctgctact ttaagagcta gtttgaagcg tgagtttgac
     2101 cagatttggt catctaccac gccagatggt ttttgggtcg ctaggattaa gtgaatccct
     2161 agggaacgac caattcgcgc tgttgagaca agctctttca taaactcagg ttggttggat
     2221 ttcaattcgg caaactcatc ggagattaag aagagatgag gcattggttc tgaaacttct
     2281 ccaagtttgt atttcttctg atactggtta atatgattaa catcagcttt agcaaataaa
     2341 cgttgacgac gtttcaattc agcattaata gaaaccaaag cacgcatcga ttgggcacca
     2401 tctaagttgg tgatggtgcc aagtaagtgt ggcaggtcct taaagagatt tgccattccc
     2461 ccacctttgt agtctatgag aaggaaagct acatcgtgtg gatggaaatt aacagctaaa
     2521 gaaaggatat aggactggat aatttctgac ttccctgatc ccgttgttcc tgcaactaat
     2581 ccatgaggac catgagcttt ttcatgtagg tttagataaa caatatcatc ctgaccacgt
     2641 aagccaagtg gaacagctaa gcttttatac ggagcatggc ttatccaacg ctcttgaaca
     2701 tgcaaatctt caaattcttc tgcttgatac atttccataa aagtaactgc ttctggtatt
     2761 gaagatttaa gatttcttgc gctgtcgtag tatgagggac gactgtaata tgacattgac
     2821 gttgtgacag ttctcttaga atagaatgtt ttaaaccaaa gtcaactaat acaatatttt
     2881 tgccaactcc aggcgaagca taagctaatt tggttgatac ttgttcaatt tgattattta
     2941 aaagaacact tttttgaagg ttttctagtg cttcatgaat agaatttccc tcgtttatta
     3001 aacaagcttt cattgttcca tgttttcgaa taatttttgt taatgctcga gtatctatac
     3061 ctgaaattcc aggtattcct tttaatttta aaaattcatc aaatgtcatc tgttgacgcc
     3121 agttacttgg atactctgcc cattcataaa caattactcc cttacatgtt ggtctaatag
     3181 attcgtaatc gtctcgattt atcccataat ttccaataag aggataagta aaagtaatga
     3241 tttgaccgtt gtaagattga tctgttatag attcttgata ccctgtcatt cctgtactaa
     3301 aaacaatttc accacttgtt cctacatccg ctccgaaagc ctcaccctca aacacactac
     3361 catcttccaa taataataga cacttcattt tatctccttt attatctgct attgagcact
     3421 gcttctaaga tagctatcct tgcatagacg ccattactca tctgtttcac tattcgagcc
     3481 ttatctgctt ctactaaatc gctagcaatc tctacatccc tattgacggg tgctgggtgc
     3541 ataataattg ctgtatccct taaatgttta tagcgttctt ttgtaagacc aaattgttga
     3601 tgatagcttt cttttgaaaa caccccttta ccatcatgac gttcgtgttg aacacgtaaa
     3661 agcataagga catcaatttg atcaactaac tgatctattg gtaaacattg cccatattca
     3721 tcgaactgac tactatacca ttctttggat ccagaaaaga aaatttcagc tcctaatcgt
     3781 tttagcactt gcatgttaga tttagctacc cgtgagtgag ttagatctcc tacaatcgca
     3841 atttttagtc ctttgaagta tccaaattct tcgtaaatag ttaccaaatc caaaagtgat
     3901 tggcttggat gttggcctga gccatcaccc ccattgacaa tagctgaatg aatgttcgga
     3961 ctagcaatca attctttata atagtcaata tcagggtgcc taatgacaca aacgtccaat
     4021 cctagagcac tcattgttaa gatagtgtca taaagggttt ctcctttatt aacagaactt
     4081 gtatcagcat taaattcaat tgtttttaat cctaatttga gttctgccat ttcaaatgac
     4141 ttatgtgttc ttgttgaatt ctcaaaaaag agatttgaca catagtaatc tttatcaaaa
     4201 ccaatgttgc taggattttc tttaacttcg atacttctct ttatcaaaga cataacttct
     4261 tgatttgata attcttctaa actgacaaaa tgttctaatg aaagtgtttg tgtttgatga
     4321 ttcatgataa tttctttcta taaattttag ctagaaaaaa gcctcctaaa acaaggaggc
     4381 tctgcaaaat agtaagctag atagggaact aacttactat ttgttaacgc aattccttgt
     4441 ttgcctctct ggacaatacc ttaaaggata ttaataagaa atgttttacc tataccaatg
     4501 tcgctgcgtg gctatctttt tgatagacta tttcaccatt gcaaattgtg tagtgaacaa
     4561 ctcccttcaa tttatcaccg ataaaaggag agttgctgga tttggaggca aattctgcct
     4621 ttattattct ctcttcagct tgatcaaaaa taacgatatc tgcaggtcca ccttctctga
     4681 gataacctgc atcaaatcca tataactgtg caggattaca tgtcattttt gccaaaaagt
     4741 caatcattga gagttcttta gtagatacta aataagtaat ccctaaggct aatgatgtct
     4801 ctaagccagt cataccagag ggcgcttgac taatattatc tgctgctttc tccataatat
     4861 gatggggagc gtgatcactt gcaataattg aaataacacc acttttaaga ccatcaatga
     4921 gggcttgtct atccttttca agtcgcagtg gtggatttaa ttttgcatta gcacccttag
     4981 ttaaaagtaa gtcctctgtc tttgaaaaat gttggggggt tacttctgct gttaagttag
     5041 caccaagctt ctgcgcaaaa tcaactactt ctactgattc tgaacttgac aaatgctgga
     5101 tatggacttt cgcctgtgtt tggtaagcaa tcatagcatc tcgcgcaatc attgaatatt
     5161 cagcaagacc actagctcca cagacatgat aaattttctg agcaatatgt tcgttgatac
     5221 caagaacacc atttaacgat ggatcctctt catgtaacga taggacaaca tcatgttttc
     5281 tagctaagtt catagcttct tgcaaaacct tagtatctgt caaagggata ccatcgtctg
     5341 agaatccagc aactccagct tctaataatt cttcgaaatt aactaaatct ttaccattca
     5401 gggatttagt aattgtagca actgtttcaa tcttaattgc ttcttttgct gcactctcct
     5461 taacttgttt tacaatttct gagctagaaa tcgttgggtt agtattggcc atcataagaa
     5521 ctgtcgtaaa accacctgct gcagctgata aggctcctgt atggatattt tctttatgtg
     5581 tctgtccagg ctcacgaaaa tgtacatgaa tatcgactaa tcctggagct acaattttat
     5641 tgcttgcatc aatgattgga atgcctttaa tatcaatttc tttcgatatt tgtttaattt
     5701 ttccttgatc aatcagcatg tctgatactt gattaaaacc actttgaggg tcgataatca
     5761 atccgttttt aatgatatac atatctgtcc tctctaactt tgccaattca tttgatcttc
     5821 tttaaatttt ttcagtaata ccagttggtc agctgtaata tatccattaa ctttagcaac
     5881 tttaattaat tgattgtaat ccgtcaaagt caccaaagga atatcagctt cacgaaatgc
     5941 ttgttcagcc ttagctaatt gatatgtaaa tatcgctacc acccctaaaa cctcaatacc
     6001 ttgactctgc gcagccgtaa cagcctctaa aacagatcct cctgttgaaa tcaaatcttc
     6061 aatgataacc attttttggc ctgagtaaac acgtccctcg atttgattcc ccacaccatg
     6121 atcctttgcc ttcgagcgta tataggcaaa gggcaagttc atcttatcag ctataatagc
     6181 gccatgtggt atgcctgcag tcgctgttcc tgcgatgata tcaacgtttg ggaaatgttt
     6241 ctgtatttgt ttaacaaaac cattttcaat cagtgtcctt gtctcaggat acgataaggt
     6301 tacacgatta tcagtgtaaa ttggcgattt gacccccgaa gcccaagtaa agggttgttg
     6361 gggtcttaag taaacagctt gaatatctaa tagttccatg gcaatctgtc ttgctaaatc
     6421 catctcagta ctcctttatt taatacgatt ccattcttct tttatgtcta gatatgcttg
     6481 atatggattt tctgctttcg ttataggtct tcccacaacg atataatcag ccccaactat
     6541 acgtgcttgc tcaggagtca tcgttctttt ttgatcatct aactgattgg tctgtggagg
     6601 tctgatacca ggcgttaagc aaatgaaatg ttcgttagtc tgtttcttga tttgctttac
     6661 ctcttgagcc gatgcgacta ctccatctaa gcctgcttct tgagcttttt gagcataatg
     6721 gcaaaccgaa tcaactacac ttaagttaat atgttgatcc acctgcattt gttcttgtga
     6781 agtcgaggtt aattgtgtca ctgctaatag tattggaccc tcccccaaac cttctctagc
     6841 tgctttcatc atttcgactc ctcctgcagc atggacatta gtcatatcaa tgcctaattt
     6901 ggctaagaca gacatagata atcgaactgt attagggata tcatgtaatt ttaaatccaa
     6961 aaaaatgcga tgtcctaagg atttaatata tctaataatt gagggacctt cggaataata
     7021 aagctccatt cctattttga caaaaagcaa ttcttctttt ggaaaatgtt ctaaaaaagt
     7081 tgttacagaa gccaaatcag aaaaatctaa ggcaatgata gggcattttt ctaacatata
     7141 attcctctct atagaaataa taattgttgg aaatgtacca gtatgacaca aaaaaccttg
     7201 cctgagggca aggtttcata aaaagttagc gaaaaagcac tcgctatttg acttatgatg
     7261 gtgaccttgt tcgcctctct ggactcactt aaagatacat ttgtaggtat tctacaagtt
     7321 tacaatcatt ttgtcaaata aatactaaat tattttatca ttttacgtct aataaagaac
     7381 gatagaggaa tctgctgtac aatactgatt gctatagcaa ttgtaaaagc tgttacaaaa
     7441 caggatctaa aatattccat tgctaaggca tctgctcccg tcaataaaaa gtaaacggct
     7501 ctatagaagc taagtcctgg gataagagga aacatacttg tcgctaaaaa gacagtggca
     7561 ggacacttac gtttagcagc taatagccgt gaaaaataag tgatgaaaag ccctggaaaa
     7621 aatacggcta tagcatccat gtgaaattgt tgccataaga tcaggtagag catccaagat
     7681 attgctccta gtatccctaa gtcaaggaaa taacgttttg gaacatggta gagaatagag
     7741 aaggctatcg tccctaaacc ggccattagt gttctgatga taatctcaag aatggtatta
     7801 ggaacaccag aaaaagaacc tgtcatttgt tctgcaaacg gaataatttc aattgtaata
     7861 gccacgcctg ctgatataga aatacaaatg agtaaagcag acataatcaa agctaaacct
     7921 gttgaaaaat tattctgtga aaattctctc accgaattaa caaaggcagc ccctggcacc
     7981 ataaccatta atgcccctaa tatgataatg ctacgatgct cacctaatcc tgaaaaatat
     8041 aataaattag cacttaaagc cactacagaa ctacctagaa tggtcaaaag aaatcctgta
     8101 tgtatacgac tttccatcaa attcaaaaaa tatcctaaaa ttaagcctgt tacagcagcg
     8161 gaaaatgaat ctaataagga actacccaag gctaaagaaa aactacccgc tccacaaaaa
     8221 taagaaatta aagtaacaag caaactataa tctttttgat gttcaattaa gtctaactgc
     8281 tttacaattt cttctggaga tactaagtct tgtttagcaa tttgcctaga aaggttatta
     8341 acagcttcaa gtctacctaa atttgctgtt acaccatctg tattgcatac tttagcttct
     8401 tgtttaccat agcggttaat cccggaagcg ataatactgc tactaaccac ataagcttca
     8461 aaattttcta tctgaagagc cttagcaaaa tgcttcattg tgtcttcgac acgatagact
     8521 tctgctccgc tttcaatcag taatttacca gctctaagaa caacgctcat atcattttgc
     8581 atcttatttg gctctgcctt ctactttatt accctagcca cttaactaag ttggttaaag
     8641 tcccaaacat catccatcca tccttcataa aagtccggtt catgacatac catcaagatt
     8701 gatcccttat aagcttttaa agcacgtttt aactcatctt tagcatctac atctaaatga
     8761 ttggttggtt catcaagtac aaggacatta ttttcgcgat tcattaataa gcaaaatctg
     8821 acctttgatt gctccccacc agataacacc tgaatctgac tttcaatatg ctttgaagtt
     8881 aaaccacaac gagcaagagc tgccctaacc tctgcttggt tcaaagcagg aaaagcatcc
     8941 catactgctt caagtggagt ttggcgattc cctccgggta cttcttgttc aaaataacct
     9001 aaatcaataa aatcacctcg ttcaacatta ccagaaatag gggggataat acctagtaaa
     9061 ctcttaagta atgttgtttt tcctatccca tttgctccaa caatagctat tttttgatta
     9121 cgttcaaatg ttaggtttag aggttttgtt aaagcacgat catatccaat ttgaagatct
     9181 ttagcttgga aaataaaacg tcctggtgtg cgagattctt taaattcaaa acttggcttt
     9241 ggtttctctg cttgtaactc aataatatcc attttgtcta gtttcttttg acgagacatt
     9301 gccatattac gtgttgcaac gcgtgcttta ttacgattaa caaaatcttg taaatcagct
     9361 atttcctttt gttgacgttc ataggctgct tctaattgag cctttttcat tgcatagact
     9421 gattcaaaat tagtataatc accggaatat cttactaaat cttgattttc aacatgatag
     9481 acaatattga taacatcgtt caaaaatgga atatcgtgtg agattaaaac aaaagcattt
     9541 tcataatttt gtaaatagcg ttttaaccaa tcaatgtgtt cagcatcaag gtagtttgta
     9601 ggctcatcaa gcaggaggat atctggcttt tctaataaca gttttgctaa aaggaccttt
     9661 gtcctttgac caccagataa ttcagtaaca tcagaatcca taccaaaatc cataactcct
     9721 aatgcccgtg cgacttcatc aattttagca tcaagcgtat aaaaatcacg tgattctaaa
     9781 cgatcttgta gttctccaac ttcttccata aggctatcta catctgcgcc atcatctgcc
     9841 atggatatat atatatcatt gatacgtgcc tctgttttaa aaagttcatc aaatgctgtt
     9901 cgcaaaacat cacgaactgt ttgaccttct tctaacacag tatgttgatc aaggtaacca
     9961 gcagttacat attttgacca ttcaattttt ccttcatcag gttgaagatg acctgtaaca
    10021 attgacatga atgttgattt tccttcacca ttagcaccaa taagtcctat atgttcacct
    10081 tttaaaaggc gaaaagaaac attctcaaaa atagcacgat caccaaaacc gtgacttaag
    10141 ttttttactt ctaaaatact catttaaaat cctttgattg ataatagagg ttattaaaaa
    10201 actcatagag acactctaca agtttttaaa ttagcacatt acatagtaag ttccatatac
    10261 aagtatagag agtttaaact taaaagtcaa accttactta attttttcaa agtgtaaatg
    10321 aacagtgatt tgatcatcat aaccatttcg ctctaaatca cgttgcaaac ggtatgaaat
    10381 atagtgttca gcaatatgaa tgtatcctgc atccaataaa cgattttcaa ccaaagactg
    10441 aatcatattg atcgttggtc gttcaacctg agcatcctct aaatctaaga ttactttttt
    10501 agtcacttga gcaaggtctt gacgaagtga ctcatcaagc acataaactg tttgagcagc
    10561 tttcaagata gcttggtaga ttttttcagg atcaaattca acaatctgtc catcacgttt
    10621 gatgatttgc ataagagccc ctccttaatg tggaaacaat gttaaaacga tttgcaatca
    10681 ttttcatttg ttaatttcat tatgcaaatg ttttcataat ttgtcaagtg ttttacctga
    10741 tattgatgaa aaattaatat cactagaagt cagtataagt attaagcaat gtcatattct
    10801 tctatattaa ttcaaatttt aactctttcg ctggtttaac cagcccattc ttatgcaatg
    10861 tttctgcgat ttgaacagaa ttccaagctg cccccttcag tagattatct gaaactaccc
    10921 acatatgaac accattttct tgatcaaggt cttttcgaat acgaccaaca aaggtctccc
    10981 tatggcctac agcattaatc gcttgagggt aaatttgctg agatggaagg tcttgtagga
    11041 cggcaccagg aaaatttgca atagcctttt taatttcaga gattgaggct aattctttcg
    11101 tctcaatata aatagactca gaatgacccg ataaaacggg aatgcggacg caggtagctg
    11161 aaaccttaat agtagcatct tccataattt tctttgtctc caaagtcatt ttcatttctt
    11221 cgtacgtata atcattatca gtaaaaatat caatttgagg taatgcattg aatgcgatag
    11281 gataatgttt ttgatcgcta gaacatggca agaccgttgc aattaattga tcaggtgaaa
    11341 ggttatcatt taaaacttgt ctcaactgtt ccttagtttc ttcaacagca cgtgcacctg
    11401 aacccgaaac agcttgatag gtggaaacta taacacgctc tatcccccat ttttgacgaa
    11461 tgggctctaa agcaatcatc atttgaatag tagaacaatt gggacaagct atgataccat
    11521 tatgaccaat catggcatga gcatttactt caggaacaac tagtggaaca tcaggattct
    11581 gacgaaaata tgacgtgtta tctactacaa ctgctccaga ctttactgca taaggagcga
    11641 actttgctga aatagatcca ccagctgaaa acaaggcaat atcaacatcg taaaatgatt
    11701 cttttgttgt ctcttcaaca cgtatagcct catctttaaa atgtaaaatt ttacctgctg
    11761 agcgacttga tgataaaagt ttcacttgtt ctattggtaa attcgattgt tctaattgac
    11821 gaatcatttg tgttcctacg gcacctgtag cacccacgat agcaacggta tatcccatat
    11881 aatgacctct atttgttgat gttattcatc aatattttca aaacatttta atgtgactat
    11941 tatactacat ttttataaac tttcaagaat agccaaatga aaaaagtcac cctaa
//
LOCUS       DBJYSH010000064        11640 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.064, whole genome shotgun sequence.
ACCESSION   DBJYSH010000064 DBJYSH010000000
VERSION     DBJYSH010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 11640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11640
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.064"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            203..508
                     /locus_tag="ACUBV3_001318"
     CDS             203..508
                     /locus_tag="ACUBV3_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cylX protein"
                     /protein_id="HHU3847818.1"
                     /translation="MGRSNILSRCEQCSDNQCGLYKTRLKDKLLLVVQLVGNIRYSEF
                     LTEGGVYHKGETLLEVITLGISYEYKSYGDIKVIKKYCADGSVVDYNMPILLMERIE"
     gene            508..1356
                     /locus_tag="ACUBV3_001319"
     CDS             508..1356
                     /locus_tag="ACUBV3_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CylD"
                     /protein_id="HHU3847819.1"
                     /translation="MKTVIIYGGQGNVQSKHLCQLVKTPKGEILFSKLQATDLESYQL
                     MMDVINHKVSLDNVHASMLANFLYNDWLTNDEKIVSGIGFSAHSAGIFNVLYASGSTS
                     FENIIHFIKRRAQLIENCHVSEELWLLMVENIDMFSKDVLKEQSEKVKLAIITDDRAG
                     VIAMDQVNRSFFDDVMTNKGYFYKLKQLGVKAPYHTDFLNESKEQYKQLIESLDISQN
                     DDYDYIFHCDQLVDEILYQWTNLFNWRAIKETIIEKDAEIIDISPNRFISSQLQKMKK
                     RGRKQL"
     gene            1353..2075
                     /gene="fabG"
                     /locus_tag="ACUBV3_001320"
     CDS             1353..2075
                     /gene="fabG"
                     /locus_tag="ACUBV3_001320"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HHU3847820.1"
                     /translation="MKVAIVTGGTRGIGRAITKELYKEGYKVIAIYNSNDAKARALQE
                     ELPKLDVYKCNISDAKAVQKLVTKIFREYGGIDCLVNNAGIVRDGFFLMMSEEKWMDV
                     ININIMGLVNMSKAVLKIMKAKRIQGKVINISSTSGIAGQIGQANYSATKGAIISITK
                     TLAKEFASDGITINCVSPGFIETDMTNELQNKEELKEHLIPLKRFGQPEEVAWLVSFL
                     ASEKANYITGKNIVIDGGMIND"
     gene            2068..2373
                     /locus_tag="ACUBV3_001321"
     CDS             2068..2373
                     /locus_tag="ACUBV3_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HHU3847821.1"
                     /translation="MINWAEERKIIKTRQEVVDEIKGILIDSLMLDIDKDLIMNDQPL
                     FGRGLELDSIDALELSIGISTTFGVELNDDDISILSSVNRLADFVIENSEDIDDQAK"
     gene            2357..2833
                     /gene="fabZ"
                     /locus_tag="ACUBV3_001322"
     CDS             2357..2833
                     /gene="fabZ"
                     /locus_tag="ACUBV3_001322"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HHU3847822.1"
                     /translation="MTKLSSEEIIKMLPHRYPFLMVDRVQSYKKGIIECRKNITINEP
                     YFQGHFPQRPIVPGVLMIEMAAQSAALLYILDALDGSLPSLDELTAEKIAEKVGYLAS
                     VKNFKFKKIVTPGDQLNITCKSQSKLGHLLEIQVVIRDENKKEVASGRMLVSENGN"
     gene            2823..3752
                     /locus_tag="ACUBV3_001323"
     CDS             2823..3752
                     /locus_tag="ACUBV3_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HHU3847823.1"
                     /translation="MEIKLKNIGKKYGSFEALRDINLIFEEGKFYGLLGPNGAGKTTL
                     FNLLIQNFKQTSGDIKWEVDGKPLSIKDFYRHIGIVFQSNRLDDNLTVEENLISRGAL
                     YGLSKSQVRNRLKDLQTYLDITAIKKQKYGSLSGGQKRKVDIARALLPQPSLLLLDEP
                     TTGLDPQSRRDLWDAIAQLNQQSQMTVVLITHYLEEMSACDVLNVLIEGNIYYSGDIK
                     SFIEQHSTTNLNVVLKPEKSLDQLSIADFVNKCQVLSEREIVFKDISVEEMMQVISDN
                     QDKSIIETFNVEYSNLEAAYLNLLREKERKNNV"
     gene            3745..4623
                     /locus_tag="ACUBV3_001324"
     CDS             3745..4623
                     /locus_tag="ACUBV3_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HHU3847824.1"
                     /translation="MFELISRNRKVYTRDRLAFFMSFLSVIILILVYQIFLGKIQLDA
                     IKAALRSNTVSSDTIHMVNYWLIAGLTTIISMTSTLGAFGVMVSDKEKKLNEDFKVSP
                     ISNIKIELSYAIFAVLFGIVMTMFSCIFAIGIFNGFNSLLEYSAMDYLTILGIVSLGT
                     VLSAAMILPILAFIHTSSAFTTLSTIVGTFIGFISGVYLSIGSVGKALQQVMTWFPLT
                     QINSLLKQVLMKGSIAKVFDKANEATVSNYKESYGVVLRNADGERLSNHFMLIYVIAL
                     ILILLAIHFIIKKVKK"
     gene            4620..6623
                     /locus_tag="ACUBV3_001325"
     CDS             4620..6623
                     /locus_tag="ACUBV3_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cylE protein"
                     /protein_id="HHU3847825.1"
                     /translation="MKDDNKLKISEASLEDYSEVVHLFNRNHVYQFPDGRPLTVDDLD
                     LTLKVKEVTHLFLLKNHGVLIGTSAFFKFITYGCLDWNSSFSGFLLIDSKSRSGQAIT
                     YLYKTILKKITKLKFSNIYTEISNYNKPSLALSKLNGFKEYDKTYEDILHCRSLRSHL
                     PKILNTFRISNYYGKTYDISTFQIMEEIENPLEEETEIRTKVSDEEILFKAEDSASLP
                     YYLKMSLFQMEIARLDNRYVLQVDFLSEQVKRVRVKTGKYHLANLTRAHPSLTLSRFA
                     NYYYIQATVETLYGNIDVQLERRKKHYRDATICLKRTFQGYDLLISPNGSLIFEKQKR
                     KILEDSFLIFSQPLDKKLVVKEEENHITIKCFYQGALIEKIMTFTSDEEITCVYKCNQ
                     KAKEMFPKLLKQTFKLHCQEQLIRDSEGYLVNVPGSYPIEHDDFLRADKFEDRQFHYY
                     LPNEDKMISYSPPGKASNQMQFRPLCLIDTDSLSFPLTYHFRISQASSEEALINLKKQ
                     PIWDSNYQASATDLLKHISNLTLEEEKDYGIKRMIANRKHYPSHKLVLAYNQIVLPKN
                     EIPRDSELYSISFDYRIRGKFVQIRQGEHVKYDNKSYVLENSQQLVLYVASDDKYILI
                     SAKNGIFYSYKENNHLKIRCMFKRNSPYATNVSITEYRKCEEK"
     gene            6620..7573
                     /locus_tag="ACUBV3_001326"
     CDS             6620..7573
                     /locus_tag="ACUBV3_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HHU3847826.1"
                     /translation="MNTIYNTLRTDKGYKVYEGYLYEITGEECEEALDLVIPKNIVFA
                     DTDTCGYTFLLNEDGTVYDDVTFYKFDDKYWLASHKALDSYLDNINFDYTVTDISDEY
                     KMLQIEGRYSGEIAQSFYEYDISTLNFRTLIEMTYKGEKGYLARFGFSGEFGYQFFLP
                     SSIFATFVSDVCEGIAEYGDELDRYLRFEVGQPITDIYQQEEYSLYEIGYSWNLDFTK
                     EEFRGRDSLLEHIRSATVKSVGFSTKEKLASGTPVLFDDQIVGKIFWIADEKDSSENY
                     LGLMIVNQTYAHSGVTFVTEDGQILKTQSSPYCIPESWNKE"
     gene            7570..9765
                     /locus_tag="ACUBV3_001327"
     CDS             7570..9765
                     /locus_tag="ACUBV3_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000118219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HHU3847827.1"
                     /translation="MSVYVSGIGIISSLGKNYSEHKQHLFDLKEGISKHLYKNHDSIL
                     ESYTGSITSDPEVPEQYKDETRNFKFAFTAFEEALASSGVNLKAYHNIAVCLGTSLGG
                     KSAGQNALYQFEEGERQVDASLLEKASVYHIADELMAYHDIVGASYVISTACSASNNA
                     VILGTQLLQDGDCDLAICGGCDELSDISLAGFTSLGAINTEMACQPYSSGKGINLGEG
                     AGFVVLVKDQSLAKYGKIIGGLITSDGYHITAPKPTGEGAAQIAKQLVTQAGIDYSEI
                     DYINGHGTGTQANDKMEKNMYGKFFPTTTLISSTKGQTGHTLGAAGIIELINCLAAIE
                     EQTVPATKNEIGIEGFPENFVYHQKREYPIRNALNFSFAFGGNNSGVLLSSLDSPLET
                     LPARENLKMAILSSVASISKNESLSITYEKVASNFNDFEALRFKGARPPKTVNPAQFR
                     KMDDFSKMVAVTTAQALIESNINLKKQDTSKVGIVFTTLSGPVEVVEGIEKQITTEGY
                     AHVSASRFPFTVMNAAAGMLSIIFKITGPLSVISTNSGALDGIQYAKEMMRNDNLDYV
                     ILVSANQWTDMSFMWWQQLNYDSQMFVGSDYCSAQVLSRQALDNSPIILGSKQLKYSY
                     KTFTDVMTIFDAALQNLLSDLGLTIKDIKGFVWNERKKAVSSDYDFLANLSEYYNMPN
                     LASGQFGFSSNGAGEELDYTVNESIEKGYYLVLSYSIFGGISFAIIEKR"
     gene            9770..10981
                     /gene="cylJ"
                     /locus_tag="ACUBV3_001328"
     CDS             9770..10981
                     /gene="cylJ"
                     /locus_tag="ACUBV3_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase CylJ"
                     /protein_id="HHU3847828.1"
                     /translation="MSKFLFAPFMMNLGESQRLSKLANCLYEQGHDIHILGDNYYPFL
                     FNNDAYRYHHCVEDKSVYNSKRYDAFFSLSTDFNFLSEEEIERICSIERDLLRQEKFD
                     AVLTGYRLSIVTSCRLESIPLIWIISGATHISEIVENSEGILPNGKISKASKPQTKDF
                     IKRVITTYSTNVKTWNNYIKKYGGKPFNNALELFTGDLNLVTDYSLFYEFDKDSSYKT
                     IGPILIDNVGFSKCSQINQDNKTVLLSFGTSFKRDWVESFLKTLPRHYHYLLTSCGEA
                     INIPGSYIEIVDFIDFKTIDKEIYFAIIHGGQGTVYAMAAQGIPFIGIPFFNEQFWNI
                     KKFSKEKCAYLVKKPDVREIRKAISCLERDYDIYKSYMIRLSQGIKEEAEASLQKAEA
                     EIESFIRKRQS"
     gene            10950..11525
                     /locus_tag="ACUBV3_001329"
     CDS             10950..11525
                     /locus_tag="ACUBV3_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CylK protein"
                     /protein_id="HHU3847829.1"
                     /translation="MNPLSEKDKVNYIMIYKNRQSTLENNLSGDRLMRYQQANRQVSK
                     DKLLTGSYFVYQCLKKLGFTNKICQESFSSVSSYLIGLPKGKISYSNSGDYHILTYAP
                     SGSTGVDIEKYKDRSEQTYQNYLGESVSSDMDSKLLFYKAWLQKEISYKCGKSIDITY
                     QTMIDGYIYGYAFDNTFKVEAVYLKELLPDN"
BASE COUNT         3978 a         1643 c         2249 g         3770 t
ORIGIN      
        1 gtgttttact catgattttg tgaattcaac acttaagtca tgacctcatt ttaaagagtt
       61 tcagacgaca cagaaaggaa gaaaacataa gaaaggcaat gcacggcttg tccatagtag
      121 tgtttgaagt agatgtttag aaatatttta ttttccaaca gtactgatat cagagcgaag
      181 atgattagtt gaaggtgata atattggacg ttctaatatt ttgagtaggt gtgaacaatg
      241 ttctgataat caatgtggct tgtataaaac gcggcttaaa gacaagttac tattagtagt
      301 ccaactggtt ggaaatatta ggtattctga gtttcttacg gaaggtggtg tatatcataa
      361 gggagaaacc ttgttggaag ttataacctt aggaatttct tatgaatata aaagttatgg
      421 ggatatcaaa gtaatcaaga agtactgtgc tgatggcagt gtcgttgatt ataacatgcc
      481 aatactatta atggaaagga ttgaataatg aaaacagtaa taatatatgg aggacagggt
      541 aatgttcagt caaaacacct ttgtcaacta gttaaaactc caaaaggtga aattcttttt
      601 tcaaagttac aagcaactga tttagaatca tatcagttaa tgatggatgt cattaaccac
      661 aaggtaagtt tagacaatgt tcatgcgtct atgttagcaa attttttata taatgattgg
      721 ctaacaaacg atgaaaaaat agtatctggg ataggatttt cagctcatag cgccggtatt
      781 ttcaatgtgt tatatgctag tggtagtact agttttgaaa acattattca ctttattaaa
      841 cgtcgtgccc aacttataga aaattgtcat gtgtctgaag agctttggct tttgatggtt
      901 gaaaacattg atatgtttag taaggatgtt ttaaaagaac agtctgagaa agtaaaatta
      961 gctattatta ctgatgatag agctggtgta attgctatgg atcaagtcaa ccgttctttc
     1021 tttgatgacg tgatgactaa caaaggttat ttttataaat taaagcagtt aggcgtaaaa
     1081 gcaccatatc atactgattt tttaaatgaa tctaaagagc agtataaaca attgattgaa
     1141 agcctagata tttcgcaaaa tgatgactat gactatattt ttcattgtga tcaattggta
     1201 gatgaaattt tatatcagtg gaccaatctc tttaactggc gtgcgattaa ggaaactatc
     1261 atagaaaagg atgcggagat tattgatatt tctcctaata ggtttatttc tagtcagttg
     1321 caaaaaatga aaaagagggg gaggaaacag ttatgaaagt agcaatagtt actggtggta
     1381 ccagaggaat tggaagggct attacaaaag agttatacaa agaaggttac aaagtcattg
     1441 ctatttataa tagcaatgat gctaaggcaa gagctttaca agaggagtta ccaaaattag
     1501 acgtttataa atgcaatatt tcagatgcaa aagcagtaca aaaattggtg actaaaattt
     1561 ttagagaata tggcggtatt gattgtttag ttaataatgc tggtattgtt agagatggct
     1621 ttttcttgat gatgtctgag gaaaaatgga tggatgtcat taacataaat attatggggc
     1681 tagttaacat gagtaaagcc gtgttaaaaa tcatgaaggc taagcgtata cagggaaagg
     1741 ttatcaatat ttcttcaact agtggtatag cagggcagat agggcaagcg aactattcag
     1801 caacgaaagg cgctatcatt tccattacta aaactctagc gaaagaattt gcatcagatg
     1861 gtattacgat taattgtgta agtccaggat tcattgaaac agatatgaca aatgaattgc
     1921 aaaataaaga agagctaaaa gagcatctta ttcctctaaa aaggtttgga caacctgaag
     1981 aggtggcttg gctcgtaagc tttttagcaa gtgagaaggc aaactatatt acaggaaaaa
     2041 atattgtgat tgatggagga atgattaatg attaactggg ctgaagaacg taaaataata
     2101 aaaacacgtc aagaagtagt tgatgaaatt aaaggtattt tgattgactc tttaatgttg
     2161 gatattgata aagatctgat tatgaatgat caaccattat ttggtcgtgg attagagcta
     2221 gactctattg atgccctaga actgtcaata gggatttcaa caacgtttgg tgttgaacta
     2281 aatgatgatg atatttcaat attatcatca gtaaatagac tagcagattt tgtgattgaa
     2341 aatagtgagg atatagatga ccaagctaag tagtgaagaa attataaaaa tgttacctca
     2401 tcgctatccg tttctaatgg tggatagagt gcagagttat aaaaaaggta tcattgaatg
     2461 tcgtaagaat attactatca acgaacctta ctttcaagga cattttccac aacgtcctat
     2521 agttccgggt gttttgatga ttgagatggc agctcaatct gcagctttac tttatatttt
     2581 agatgcactt gatggtagcc tcccctctct tgatgaatta acagcagaga aaatagcaga
     2641 aaaagtaggt tatttagcca gtgtgaagaa tttcaaattc aagaaaattg ttactccagg
     2701 agaccagcta aacattacat gtaaatctca atcaaaattg gggcatcttt tagaaattca
     2761 agtcgttatt agagatgaga ataaaaaaga ggttgcctca ggaaggatgt tggtatcaga
     2821 aaatggaaat taaactcaaa aatattggaa aaaaatatgg ttcttttgaa gcattgagag
     2881 acattaatct catctttgaa gagggaaaat tttatggcct tcttggacct aatggagctg
     2941 gtaaaacaac tttatttaac cttttaattc aaaattttaa gcaaacgtca ggcgatatta
     3001 agtgggaggt ggatggaaaa cctctatcta ttaaagattt ttatcgtcac attggtattg
     3061 tcttccaaag taatcgctta gatgataatt taacggttga ggaaaatcta atttctagag
     3121 gtgctttgta cggtttatct aaatcacaag tacgtaatcg ccttaaagat cttcaaacgt
     3181 atctagatat cacagcaata aaaaaacaaa aatatggcag tttatcagga ggtcaaaaac
     3241 gaaaagttga tattgcacgc gccttgctgc cacaaccttc tctattatta ttagatgaac
     3301 caacgactgg tttagaccct caatctcgtc gcgatttatg ggatgctatc gcacaactaa
     3361 atcaacaatc gcagatgaca gttgtactaa ttactcatta tcttgaagaa atgtctgctt
     3421 gtgatgtttt aaatgttctt attgaaggaa acatttatta ttcgggagat attaaaagct
     3481 ttattgagca acattccaca acaaacttga atgtggtatt gaaaccagag aagtcattag
     3541 accaactttc gatagctgat tttgttaata aatgtcaagt cctttcagaa agggagatag
     3601 tatttaagga tatttctgtt gaggaaatga tgcaggttat ctcagacaat caagataaat
     3661 ctattattga gacatttaat gttgaatatt cgaacttaga ggcggcgtat ctcaacttgc
     3721 ttagagaaaa agaaaggaaa aacaatgttt gaactaattt ctagaaatcg taaagtttac
     3781 acgcgagatc ggttagcgtt tttcatgtct tttttatcag ttattatctt aattttggtc
     3841 tatcagattt tcttgggaaa aattcaatta gatgctataa aagcagcatt gaggagtaat
     3901 acagtatcca gtgatactat tcacatggtt aattattggc tcattgcagg tttgacgact
     3961 attatttcaa tgactagtac attaggtgcc tttggagtta tggtgtccga caaagaaaaa
     4021 aaattaaatg aagattttaa ggtcagtcct atatcaaata tcaaaattga attgtcctat
     4081 gctatatttg ccgttttatt tggtattgtc atgacgatgt tctcttgcat ctttgctatc
     4141 ggtattttta acggctttaa ttccttgcta gagtattcag ctatggatta tttaactatt
     4201 ttagggatcg tttccttagg gactgtttta tctgcggcga tgattttacc tattttagcc
     4261 tttattcata ctagttcagc ttttacgact cttagcacaa ttgtagggac ttttattggt
     4321 tttatttcag gagtatatct ctcaattggc tcggtaggaa aagcattaca acaggttatg
     4381 acatggtttc cattgactca aatcaattct cttttgaaac aggtcttaat gaagggttct
     4441 attgcgaagg tatttgacaa agccaacgaa gccactgtct ctaactataa agaatcatat
     4501 ggtgttgttt tgcgtaatgc tgatggagaa aggctgagta atcactttat gttgatttat
     4561 gtcattgccc tcattcttat tttattggca attcatttta ttattaaaaa ggttaaaaaa
     4621 tgaaagatga taataaatta aagatttctg aagcttcctt agaagattat tctgaagtgg
     4681 ttcatttatt taataggaat catgtttatc aatttccaga cggtaggcct ttaactgttg
     4741 atgaccttga tttaacgtta aaagttaaag aagtgacaca tttattttta ctgaaaaatc
     4801 atggtgtttt gattggaaca tcagctttct ttaaatttat tacctatggt tgtttagatt
     4861 ggaatagtag ctttagtggt tttcttttaa ttgattcgaa aagtcgtagt ggacaggcaa
     4921 tcacttatct atataaaact atcttaaaga agataacaaa actgaagttt tctaatatct
     4981 ataccgaaat cagtaactac aataaaccct ccttagcttt atcaaaattg aatggcttca
     5041 aagagtatga taagacatat gaagacatac tgcattgtcg atcattgcgt agtcacctcc
     5101 ctaagatatt gaatacgttt cgtatttcga attattatgg taaaacatac gacatatcaa
     5161 cttttcaaat tatggaggaa attgaaaatc ccttggaaga ggagacagaa attaggacca
     5221 aagtctctga tgaagaaata ttatttaaag cagaagatag tgcttcactt ccttattatt
     5281 taaaaatgag cttgtttcaa atggaaattg ctaggttaga taaccgttat gttttacaag
     5341 ttgacttttt atcggaacaa gtaaagaggg ttcgggttaa aactggaaaa tatcacttgg
     5401 caaacttaac gagggctcac cctagcctaa ctttaagtcg ttttgccaat tattattaca
     5461 ttcaagcaac tgtggaaacc ctttatggta atatcgatgt tcaactggaa cgtcgaaaaa
     5521 agcattatag agatgcaact atttgtctta agagaacttt tcaaggttat gatttactta
     5581 tctctccaaa tggcagcctt atttttgaaa agcagaaaag aaagatactt gaagatagtt
     5641 ttttgatttt tagccaacct ttggacaaga aattagttgt aaaagaagaa gaaaatcaca
     5701 ttacaatcaa atgtttctat caaggtgctt taattgagaa aatcatgaca tttacaagtg
     5761 acgaagaaat cacttgtgtt tataaatgta atcaaaaggc taaagagatg ttcccaaaat
     5821 tattgaaaca gactttcaag ttacactgcc aagagcagtt gattagagat agtgaaggtt
     5881 atcttgtgaa tgttccagga agttacccga ttgagcatga tgattttctt cgagcagata
     5941 agtttgaaga tagacaattc cattattacc tcccgaatga agataaaatg atttcctatt
     6001 ctcctcctgg caaagccagc aatcaaatgc aatttagacc cttatgtctt attgatacag
     6061 atagcctttc ttttccactg acttatcatt ttaggatttc acaagcatca tcggaggagg
     6121 cgttaatcaa tctaaaaaaa cagccaatat gggacagcaa ctaccaagcg tcagcaactg
     6181 accttttaaa acatatctct aacctaacat tagaagaaga gaaggattat ggcatcaaga
     6241 gaatgatagc taacagaaag cattatccta gtcacaaact ggttttagcc tataatcaga
     6301 ttgttttgcc taagaatgaa attccaagag acagtgaatt atattctatt tcttttgatt
     6361 atcgaattag aggaaagttt gtccaaatcc gccaagggga acatgtcaaa tatgataata
     6421 aatcctatgt tttggaaaat agtcaacaat tagtattgta tgtagctagc gatgataaat
     6481 atatactaat ttctgctaaa aatggaatat tttattctta caaagagaac aatcatttaa
     6541 aaataaggtg catgtttaaa aggaactcac cttatgctac aaatgtgagc attacagaat
     6601 atagaaagtg tgaagaaaaa tgaatactat ttataataca ttgagaacag ataaaggtta
     6661 taaagtttat gaggggtatt tatatgaaat tactggtgaa gaatgtgaag aagccttaga
     6721 ccttgtgatt cctaagaata ttgtatttgc agatacagat acttgtggct acactttttt
     6781 actcaatgaa gatggaacag tttatgatga tgtgactttc tacaaatttg atgataaata
     6841 ttggttggct agtcataaag ctttggattc ttatttagac aacatcaatt ttgactatac
     6901 cgtaacagat atttctgacg agtataaaat gctgcaaatt gaaggaagat attcgggaga
     6961 aattgctcag tcattttatg aatatgatat ttcaacactt aattttcgta ctttgataga
     7021 gatgacttat aaaggtgaga aaggttatct tgctagattt ggtttttctg gagaatttgg
     7081 ctatcaattt ttcctaccat cttctatttt tgctactttt gtttcggatg tctgtgaagg
     7141 tatagcagag tatggggatg aacttgatag atatttaagg tttgaagtgg gacaacccat
     7201 tactgatatt tatcaacaag aagaatattc tttatatgaa ataggttatt cttggaatct
     7261 agatttcaca aaggaagaat ttagaggtcg cgatagcttg ttagagcaca tcagatcagc
     7321 aacagttaaa agtgttggat tctcaacgaa ggaaaaactc gcttcaggaa caccagtgct
     7381 atttgatgac caaattgttg gaaagatttt ttggatagca gacgagaaag actcttcgga
     7441 aaattaccta ggtttgatga ttgttaacca aacatatgct cattcaggag ttacttttgt
     7501 aacagaagat ggccaaattt tgaaaacaca atcaagccct tattgtatcc cagaaagttg
     7561 gaacaaagaa tgagcgtata tgttagtgga ataggaatta tttcttcttt gggaaagaat
     7621 tatagcgagc ataaacagca tctcttcgac ttaaaagaag gaatttctaa acatttatat
     7681 aaaaatcacg actctatttt agaatcttat acaggaagca taactagtga cccagaggtt
     7741 cctgagcaat acaaagatga gacacgtaat tttaaatttg cttttaccgc ttttgaagag
     7801 gctcttgctt cttcaggtgt taatttaaaa gcttatcata atattgctgt gtgtttaggg
     7861 acctcacttg ggggaaagag tgctggtcaa aatgccttgt atcaatttga agaaggagag
     7921 cgtcaagtag atgctagttt attagaaaaa gcatctgttt accatattgc tgatgaattg
     7981 atggcttatc atgatattgt gggagcttcg tatgttattt caaccgcctg ttctgcaagt
     8041 aataatgccg taatattagg aacacaatta cttcaagatg gcgattgtga tttagctatt
     8101 tgtggtggct gtgatgagtt aagtgatatt tctttagcag gcttcacatc actaggagct
     8161 attaatacag aaatggcatg tcagccctat tcttctggaa aaggaatcaa tttgggtgag
     8221 ggcgctggtt ttgttgttct tgtcaaagat cagtccttag ctaaatatgg aaaaattatc
     8281 ggtggtctta ttacttcaga tggttatcat ataacagcac ctaagccaac aggtgaaggg
     8341 gcggcacaga ttgcaaagca gctagtgact caagcaggta ttgactacag tgagattgac
     8401 tatattaacg gtcacggtac aggtactcaa gctaatgata aaatggaaaa aaatatgtat
     8461 ggtaagtttt tcccgacaac gacattgatc agcagtacca aggggcaaac gggtcatact
     8521 ctaggggctg caggtattat cgaattgatt aattgtttag cggcaataga ggaacagact
     8581 gtaccagcaa ctaaaaatga gattgggata gaaggttttc cagaaaattt tgtctatcat
     8641 caaaagagag aatacccaat aagaaatgct ttaaattttt cgtttgcttt tggtggaaat
     8701 aatagtggtg tcttattgtc atctttagat tcacctctag aaacattacc tgctagagaa
     8761 aatcttaaaa tggctatctt atcatctgtt gcttccattt ctaagaatga atcactttct
     8821 ataacctatg aaaaagttgc tagtaatttc aacgactttg aagcattacg ctttaaaggg
     8881 gctagaccac ccaaaactgt caacccagca caatttagga aaatggatga tttttccaaa
     8941 atggttgccg taacaacagc tcaagcacta atagaaagca atattaatct aaaaaaacaa
     9001 gatacttcaa aagtaggaat tgtatttaca acactttctg gaccagttga ggttgttgaa
     9061 ggtattgaaa agcaaatcac aacagaagga tatgcacatg tttctgcttc acgattcccg
     9121 tttacagtaa tgaatgcagc agctggtatg ctttctatca tttttaaaat aacaggtcct
     9181 ttatctgtca tttcgacaaa tagtggagcg cttgatggta tacaatatgc caaggaaatg
     9241 atgcgtaacg ataatctaga ctatgtgatt cttgtttctg ctaatcagtg gacagacatg
     9301 agttttatgt ggtggcaaca attaaactat gatagtcaaa tgtttgtcgg ttctgattat
     9361 tgttcagcac aagtcctctc tcgtcaagca ttggataatt ctcctataat attaggtagt
     9421 aaacaattaa aatatagcta taaaacattc acagatgtga tgactatttt tgatgctgcg
     9481 cttcaaaatt tattatcaga cttaggacta accataaaag atatcaaagg tttcgtttgg
     9541 aatgagcgga agaaggcagt tagttcagat tatgatttct tagcgaactt gtctgagtat
     9601 tataatatgc caaaccttgc ttctggtcag tttggatttt catctaatgg tgctggtgaa
     9661 gaactggact atactgttaa tgaaagtata gaaaagggct attatttagt cctatcttat
     9721 tcgatcttcg gtggtatctc ttttgctatt attgaaaaaa ggtgataaaa tgagtaaatt
     9781 tttatttgct cctttcatga tgaatttagg agagagccaa agattatcaa aactggctaa
     9841 ttgtttatat gaacaaggac atgatattca tatccttgga gacaattatt atccattttt
     9901 atttaataat gatgcttatc gctatcacca ctgtgtagaa gataaatctg tttataatag
     9961 taagcgatat gatgctttct tttctctttc aacagacttt aattttttat cagaagaaga
    10021 aattgaacgc atctgttcta ttgaaagaga cttgttacgc caagaaaaat ttgatgctgt
    10081 attgacaggt taccgtctat ctattgttac aagttgtcgt ttagaaagca taccattaat
    10141 ttggattatt tcaggggcaa cgcatatcag tgaaattgtt gaaaactcag aaggaatatt
    10201 gcctaatggg aagataagta aagctagtaa gcctcaaaca aaggatttta tcaaaagagt
    10261 aatcactact tattcaacaa atgttaaaac gtggaataac tatattaaaa aatatggtgg
    10321 caaacctttt aataatgctt tagaattatt tactggtgat ctaaacttgg taacagatta
    10381 ctctttgttt tatgaatttg ataaagattc gtcctataaa acgataggac ctattttgat
    10441 agataatgta ggtttttcaa aatgcagcca aattaatcaa gacaataaga ctgtactgct
    10501 cagctttggt acttcattta aacgagattg ggtggaatct tttttaaaga cattaccaag
    10561 gcattatcat tatttactga caagctgtgg tgaggctata aacattccag gaagttatat
    10621 tgagatagtt gattttattg attttaaaac catagataaa gagatttatt tcgctattat
    10681 acacggtggg caaggaacag tttatgcgat ggctgcacaa ggtattccct ttataggtat
    10741 ccctttcttt aacgaacaat tttggaatat taagaagttc agtaaagaaa agtgcgctta
    10801 tttggtaaag aaacctgatg taagagagat acgtaaagca attagctgtc ttgaaagaga
    10861 ctatgatatc tataaaagtt atatgataag actaagccaa ggaattaaag aagaagcaga
    10921 ggcatcttta caaaaagcgg aggcagaaat tgaatccttt atcagaaaaa gacaaagtta
    10981 actatattat gatttataaa aataggcagt ctacattgga gaataattta tctggcgatc
    11041 ggttgatgag atatcaacaa gcaaatagac aggtaagcaa agataaactg ttaacaggga
    11101 gttattttgt gtaccaatgc ttaaaaaagt taggttttac caataagatt tgtcaggaat
    11161 ctttttctag tgttagtagt tatttaattg gtttgccaaa ggggaaaatt agttactcta
    11221 attctggtga ctatcatatt ctaacctatg ctcccagtgg ttcaactggg gttgatattg
    11281 agaaatacaa agatagatca gaacaaacct accaaaacta cctaggagaa tcagttagta
    11341 gtgatatgga ttctaaatta ttattttata aggcatggtt acaaaaagaa atttcctata
    11401 aatgtgggaa atctatagat attacctatc aaacaatgat agatggctat atttatggtt
    11461 atgcttttga taatactttt aaagtagaag cggtttactt aaaagaatta ttaccagata
    11521 actaactcgc aacatgttgt tcctacatat gcatctataa aaagcacttt gaagtatttc
    11581 aagggctttt tataatacct tgggtagtct ttaacaatta tggttagagt tgatatgata
//
LOCUS       DBJYSH010000065        11364 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.065, whole genome shotgun sequence.
ACCESSION   DBJYSH010000065 DBJYSH010000000
VERSION     DBJYSH010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 11364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11364
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.065"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            complement(24..857)
                     /locus_tag="ACUBV3_001330"
     CDS             complement(24..857)
                     /locus_tag="ACUBV3_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease subunit"
                     /protein_id="HHU3847830.1"
                     /translation="MFTKFFEGLLTYHFLQNAFITAIVIGIVAGAVGCFIILRSMSLM
                     GDAISHAVLPGVAISFILGINFFIGAIVFGLLSSIIITYIKENSVIKGDTAIGITFSS
                     FLALGIILIGLANSTTDLFHILFGNILAVQDSDKYMTIIVGLIVLTLITIFFKELLLT
                     SFDPVLAKSMGMRVSFYHYLLMILLTLVAVTAMQSVGTILIVALLITPAATAYLYVKS
                     LRTMLFLSSALGAVASVLGLYIGYTFNIAAGSSIVLTSTFMFLLAFLFSPKQSLFKKK
                     Q"
     gene            complement(859..1575)
                     /locus_tag="ACUBV3_001331"
     CDS             complement(859..1575)
                     /locus_tag="ACUBV3_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HHU3847831.1"
                     /translation="MIISKHLSVSYDNNLVLEDINLRLEGSGIIGILGPNGAGKSTLM
                     KALLGLVDSTGESGIGGDLLPLMGRVAYVEQKTNIDYQFPITVGECVSLGLYKERGLF
                     KRLSKTDWEKVSRVIDQVGLRGFENRPINALSGGQFQRMLMARCLVQEADYIFLDEPF
                     VGIDSISEQIIVNLLKKLSKAGKLILVVHHDLSKVDHYFDQVIILNRHLIACGPIDQA
                     FTRENLSAAYGDAILLGQGD"
     gene            complement(1746..2672)
                     /locus_tag="ACUBV3_001332"
     CDS             complement(1746..2672)
                     /locus_tag="ACUBV3_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein"
                     /protein_id="HHU3847832.1"
                     /translation="MKKWLVIVSCFVAFLGLGACANKQDQAKNEKLKVVVTNSILADI
                     TKNIAGNKIDLHSIVPVGKDPHEYEPLPADVEKTTQADLVFYNGINLETGGNAWFTKL
                     IKNAKKKENKDYFAVSDGVDVIYLNGQSGKGKEDPHAWLNLENGIIYSKNIAKQLIAK
                     DPKNKATYEKNRDAYVAKLEKLDKEAKSKFNAIPANKKLIVTSEGCFKYFSKAYGVPS
                     AYIWEINTEEEGTPDQITSLVKKLKQVRPSALFVESSVDKRPMKSVSRESGIPIYAEI
                     FTDSIAKKGQKGDSYYAMMKWNLDKIAEGLAK"
     gene            2839..3486
                     /locus_tag="ACUBV3_001333"
     CDS             2839..3486
                     /locus_tag="ACUBV3_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HHU3847833.1"
                     /translation="MTPNKEDYLKCIHELGEKNNKISNKKIAEMMQVSAPAVSEMIKK
                     MISEQLIVKDKDLGYYLTKQGLLVVSDLYRKHRLVEVFLVNHLHYTADDIHEEAEVLE
                     HTVSTTFVDQLEKLLDFPQFCPHGGTIPKKGEFLVEINQMTLDQISQLGTYVISRVHD
                     DFQLLKYLEQHRLHINDTIELTQIDPYAKTYHIIYNDENLTIPERIASQIYVSAT"
     gene            complement(3526..4215)
                     /locus_tag="ACUBV3_001334"
     CDS             complement(3526..4215)
                     /locus_tag="ACUBV3_001334"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HHU3847834.1"
                     /translation="MKIGIIAAMEEELKLLVENLEDKSQETVLSNVYYSGRYGEHELV
                     LVQSGVGKVMSAMSVAILVESFKVDAIINTGSAGAVATGLNVGDVVVADTLVYHDVDL
                     TAFGYDYGQMSMQPLYFHSDKTFVSTFEAVLSKEEMTSKIGLIATGDSFIAGQEKIDV
                     IKGHFPQVLAVEMEGAAIAQAAQATGKPFVVVRAMSDTAAHDANITFDEFIIEAGKRS
                     AQVLMAFLKAL"
     gene            complement(4225..4494)
                     /gene="macP"
                     /locus_tag="ACUBV3_001335"
     CDS             complement(4225..4494)
                     /gene="macP"
                     /locus_tag="ACUBV3_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall synthase accessory phosphoprotein
                     MacP"
                     /protein_id="HHU3847835.1"
                     /translation="MGKPLLTDDMIERSNRGEKVSGQTILDQETKIISTEDGMEQLTD
                     ENGKHIYKSRRIENAKRNEFQRKLNLVLFILLILLALLFYAIFKL"
     gene            complement(4494..5048)
                     /locus_tag="ACUBV3_001336"
     CDS             complement(4494..5048)
                     /locus_tag="ACUBV3_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HHU3847836.1"
                     /translation="MDFEEKTINRQTVFDGQIIKVAVDDVELPNGLGQSKRELVFHGG
                     AVATLAVTPEHKIVLVKQYRKAIEGISYEIPAGKLETGESGSKEEAALRELEEETGYT
                     GNLEILYSFYTAIGFCNEKIVLYLATDLQKVENPRPQDDDEVLELLELSYEDCMQMVE
                     KGMIQDAKTIIALQYYGLKMGGHS"
     gene            complement(5069..6448)
                     /gene="glmU"
                     /locus_tag="ACUBV3_001337"
     CDS             complement(5069..6448)
                     /gene="glmU"
                     /locus_tag="ACUBV3_001337"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002899862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HHU3847837.1"
                     /translation="MSNYAIILAAGKGTRMKSDLPKVMHKVSGITMLEHVFRSVQAIE
                     PSKIVTVIGHKAELVRDVLGDKSEFVMQTEQLGTGHAVMMAEEELATSKGHTLVIAGD
                     TPLITGESLKNLIDFHVNHKNVATILTADAANPFGYGRIIRNSDDEVTKIVEQKDAND
                     FEQQVKEINTGTYVFDNQSLFEALKDINTNNAQGEYYLTDVIGIFKEAGKKVGAYKLR
                     DFDESLGVNDRVALATAEKVMRQRIARQHMVNGVTVVNPDSAYIDIDVEIGEESVIEP
                     NVTLKGQTKIGKGTLLTNGSYLVDAQVGNDVTITNSMVEESIISDGVTVGPYAHIRPG
                     TSLAKGVHIGNFVEVKGSQIGENTKAGHLTYIGNAEVGCDVNFGAGTITVNYDGQNKF
                     KTEIGSNVFIGSNSTLIAPLEIGDNALTAAGSTITDNVPIDSIAIGRGRQVNKEGYAN
                     KKPHHPSQK"
     gene            complement(6692..7105)
                     /locus_tag="ACUBV3_001338"
     CDS             complement(6692..7105)
                     /locus_tag="ACUBV3_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HHU3847838.1"
                     /translation="MVKALETYIVTNGNGRQAVDFYKDVFQADLVNMMTWEEMDPNCL
                     EDRKDLIINAQLIFDGIRLQISDENPDFVYQAGKNVTAAIIVGSVEEAREIYEKLKKS
                     AQEVQLELQETFWSPAYANLVDQFGVMWQISTELN"
     gene            complement(7118..7495)
                     /locus_tag="ACUBV3_001339"
     CDS             complement(7118..7495)
                     /locus_tag="ACUBV3_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HHU3847839.1"
                     /translation="MISSIGQVMLYVSNVEASADFWKNKVGFERVEKQTQGDYVTYIV
                     APKLDSEVSFVLHDKAIIAQMSPELDLATPSILFETTDIDSTYQELTANEVMTNPIVD
                     MGSMRVFNFSDNDNNYFAIREVK"
     gene            complement(7584..8540)
                     /locus_tag="ACUBV3_001340"
     CDS             complement(7584..8540)
                     /locus_tag="ACUBV3_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HHU3847840.1"
                     /translation="MKLAVLGTGMIVKEVLPVLQKIEGIDLVAILSTVRSLETAKDLA
                     KEYNMSLATSEYKAVLDNEEIDTVYIGLPNHLHFDYAKEALLAGKHVICEKPFTLEAS
                     QLEELVSIANTRQLILLEAITNQYLPNFDLVKEHLSNLGDIKIVECNYSQYSSRYDAF
                     KRGEIAPAFNPEMGGGALRDLNIYNLHLVIGLFGEPITAQYLPNIERGIDTSGVLVLD
                     YGHFKTVCIGAKDCSAEVKSTIQGDKGSIAILGPTNTMPKISLTMNGQESHVYQLNGD
                     RHRMHDEFVIFEGIISNLDFKRAAQALEHSRTVMKVLDMVTK"
     gene            complement(8537..8988)
                     /locus_tag="ACUBV3_001341"
                     /pseudo
     CDS             complement(8537..8988)
                     /locus_tag="ACUBV3_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647214.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
     gene            complement(8985..9218)
                     /locus_tag="ACUBV3_001342"
     CDS             complement(8985..9218)
                     /locus_tag="ACUBV3_001342"
                     /inference="COORDINATES: protein motif:HMM:NF024522.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3977 family protein"
                     /protein_id="HHU3847841.1"
                     /translation="MEFGCGNTWLLRTEFEASDGTEREVKGISGDILIKSVYLRVWIG
                     QTVYILDVKEGFKRQRKMSNAVKFVVGIASEVI"
     gene            complement(9227..9925)
                     /locus_tag="ACUBV3_001343"
     CDS             complement(9227..9925)
                     /locus_tag="ACUBV3_001343"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HHU3847842.1"
                     /translation="MTKVVLVTGCASGIGYAQAQYFLKQGYQVYGVDKSDKPNLNGNF
                     NFIKLDLSSDLSPLFTMVPTVDILCNTAGILDAYKPLLEVSDEELEHLFDINFFVTVR
                     LTRHYLRRMVEKKSGIIINMCSIASFIAGGGGAAYTSSKHALAGFTRQLALDYAKDCI
                     QIFGIAPGAVQTAMTASDFEPGGLAEWVASETPIGRWTKPSEVAELTGFLASGKARSM
                     QGEIVKIDGGWSLK"
     gene            complement(9918..10154)
                     /locus_tag="ACUBV3_001344"
     CDS             complement(9918..10154)
                     /locus_tag="ACUBV3_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018367459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HHU3847843.1"
                     /translation="MTFEEILPGLKAKKKYVRTGWGGAENYVQLFDTLEVNGKVLQAT
                     PYFLINVTGEGEGFSMWAPTPCDVLAEDWIEVND"
     gene            complement(10244..10492)
                     /locus_tag="ACUBV3_001345"
     CDS             complement(10244..10492)
                     /locus_tag="ACUBV3_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3847844.1"
                     /translation="MEKITNDFEKVIAVLNQLSISYELVENGPVLLTEQADNFIVGIE
                     GVRTKIMFLTNKKKTKYYLLIMDDQKLFYPMKTIVAFR"
     gene            complement(10690..11190)
                     /locus_tag="ACUBV3_001346"
     CDS             complement(10690..11190)
                     /locus_tag="ACUBV3_001346"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000752178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HHU3847845.1"
                     /translation="MACIIRRARLGDEVNLAYIQTESWKAAFGKILPEDIIQKTTEIE
                     PAITMYQQLLHKEVGKGYILEVDSNPHCMAWWDKSREDGMLDYAELICIHSLKEGWGK
                     GYGSQMMNHVLSEIQQAGYNKVILWVFTENTRARKFYDRFGFSFKGKSKTYFEKEEMC
                     YEKILH"
BASE COUNT         3635 a         2357 c         1556 g         3816 t
ORIGIN      
        1 tctagtctca ttttttaaca acactattgt ttctttttaa acaaactttg tttaggagaa
       61 aataggaaag caagtaaaaa cataaaagta gatgttaaaa caatacttga tcctgcggca
      121 atattaaatg tataaccgat atataacccc agaacagatg ctactgctcc taatgcagat
      181 gataaaaaca acattgttct gagactttta acataaagat atgctgttgc agctggtgta
      241 attaaaagtg ctacgatcaa tattgttccc acactctgca tagctgttac tgcaactaat
      301 gttaacaaaa tcatcaataa ataatgataa aaagaaactc tcattcccat tgattttgct
      361 aaaacaggat caaaagaagt caataataat tctttaaaga agatagtaat cagagtcagt
      421 acaattaatc ccacaataat agtcatatat ttatcactat cttgtactgc caaaatattc
      481 ccaaaaagaa tatgaaagag gtcagttgta ctattagcaa gtccaattaa aatgatacct
      541 aaggctaaaa aggacgaaaa agtaattcca atagcagtat ctccttttat gacactattt
      601 tctttaatat aagtaatgat aatcgaagac aacaaaccaa agacaatagc acctataaaa
      661 aagttaatcc ctaaaataaa agatatagca acacctggta gaacagcgtg agaaatagca
      721 tcacccatca aagacattga gcgcaagata ataaaacagc ctactgcacc cgctacaata
      781 ccaatcacaa ttgcagttat aaaagcattt tgcaaaaaat gataagtcaa taagccttca
      841 aaaaatttcg taaacattct agtctccttg tcctaacaaa atagcatcac cgtaagcagc
      901 acttaaattc tcacgggtaa atgcctgatc tattggtcca caagcaatta gatgacggtt
      961 caaaataatt acttgatcaa aataatgatc aactttactc aaatcgtgat ggacgactaa
     1021 aattaatttt ccggccttac ttaatttttt tagtaaatta actatgattt gttcactaat
     1081 ggaatcaatc cctacaaacg gctcatctaa aaaaatgtaa tccgcttctt gaacgagaca
     1141 tcttgccata agcatacgtt gaaattgtcc acctgataag gcattaattg gtctattttc
     1201 aaaccctctc aaaccaactt gatcaatcac tcgggatact ttttcccagt cagtttttga
     1261 tagtcgcttg aagaggcctc gttccttata taatcctaaa gaaacgcatt ctccaacagt
     1321 tatagggaat tggtaatcaa tattagtttt ttgttcaaca tatgctactc tccccattaa
     1381 aggtagcaaa tctcccccaa tccctgattc tccagtagaa tcaaccaaac caagaagagc
     1441 tttcataagc gtagatttcc ccgccccatt aggccctaaa ataccaatga ttccggagcc
     1501 ttctaagcgt aaattaatat cttctagcac taaattatta tcataactta cgcttaagtg
     1561 tttactaata atcatttttc ctccaaaagt agaatggagc tggttataaa accagactcc
     1621 acttctctgt atttattaaa gctaagtcag tttttagatt aggatttcga cattgttaca
     1681 actcagcctt aagcgagaat actacatcat tgatttcatt atgatttcaa tgtcgattct
     1741 cactcttatt ttgctaaacc ttctgcaatc ttatcaagat tccatttcat catagcgtag
     1801 tagctgtcac ccttttgacc ttttttagca attgagtctg tgaagatttc tgcgtaaatt
     1861 gggatacctg attctcttga aacagatttc ataggacgct tgtcaacact tgattcaacg
     1921 aataaagctg aaggtcttac ttgttttaat ttcttaacta agctagtaat ttgatctggt
     1981 gtaccttctt cttcagtgtt aatttcccaa atgtatgctg atggaactcc ataagctttt
     2041 gagaaatatt taaagcaacc ttcactagta acaattaatt ttttattagc tggaatggca
     2101 ttaaatttag attttgcttc tttatccaat ttttctaatt tagctacata agcatctctg
     2161 tttttctcat aagtagcttt gttttttgga tctttagcaa ttaattgctt agcaatattt
     2221 tttgagtaaa taataccatt ttcaaggttc aaccaagcat gtgggtcttc tttacctttt
     2281 ccgctttgac catttagata aataacatca actccatcgc taaccgcaaa gtaatctttg
     2341 ttttctttct tcttagcgtt ctttattaat tttgtaaacc aagcattacc gcctgtttca
     2401 agattaattc cgttgtaaaa aacaaggtct gcttgagttg ttttttcaac gtctgctggt
     2461 aatggctcat attcatgagg atctttacca acaggtacaa tactatgtaa gtcgattttg
     2521 ttaccggcta tattttttgt tatatctgcc aaaatagagt tggttacaac tacttttaat
     2581 ttttcgtttt tggcttgatc ctgcttgtta gcacaggcac ctaagcctaa aaatgctaca
     2641 aaacatgata caataactaa ccactttttc atcaaaatgc tccttttaaa attaatataa
     2701 gttatgtatt aaattaagtt aacttaattt tgatattagc ataacctatt ataaagtatt
     2761 tgtcaaatac tttttcagta ttttttgata taatggtctt agaaaaatag attttaagaa
     2821 gtataagaaa gactgaatat gactccaaat aaagaagatt acctaaaatg cattcatgaa
     2881 ttaggtgaaa aaaacaacaa aatttccaat aagaaaatcg ctgaaatgat gcaagtctcg
     2941 gctcctgccg tgtcagaaat gattaaaaaa atgataagtg aacaactgat tgttaaagat
     3001 aaagatttag gatattatct taccaaacaa ggtttattag ttgtttctga cctttaccgt
     3061 aaacatcgtc tagtagaggt atttttagtt aaccatttgc actatactgc tgatgatatt
     3121 catgaagaag cggaagtttt agaacatact gtatcaacaa cttttgttga tcaattagaa
     3181 aagttgttag actttccaca attttgtcca cacggtggga ctattcctaa aaagggagag
     3241 tttttagtag aaattaacca aatgacattg gaccaaatta gtcagcttgg cacatatgtt
     3301 atcagtcgtg ttcatgatga ttttcagcta ctgaaatacc tagaacaaca ccgtttgcat
     3361 atcaacgata ccattgaatt gacacaaatt gacccttatg caaaaaccta tcacatcatc
     3421 tataacgatg aaaatctaac aatccctgag cgtatcgcaa gtcaaatata tgtatctgct
     3481 acataaataa aaaagcccgt gtatctaatg aatacacgga cttttttata atgctttcaa
     3541 aaatgccatt aatacttggg ctgagcgttt accagcttca atgataaatt catcaaatgt
     3601 gatattggca tcatgagctg cagtatcact catagcacgt accaccacaa agggctttcc
     3661 tgtagcttga gcagcttggg caattgctgc accttccatc tcgacagcta aaacctgtgg
     3721 aaaatgcccc ttaataacat caatcttttc ctggccagca ataaaactat ccccagttgc
     3781 aattaaacca attttactag tcatttcttc tttagataaa actgcttcaa aagtagaaac
     3841 aaaagtctta tccgaatgaa aataaagtgg ctgcattgac atttgtccat agtcataacc
     3901 aaaggcggtt aaatctacat cgtgataaac taatgtatct gcgacaacga cgtctcctac
     3961 attcaatcct gttgctactg caccagctga tcctgtatta ataatagcat caactttaaa
     4021 gctttctact aaaatagcaa cactcatcgc tgacatgact ttcccaacac cagactgtac
     4081 taaaacaagc tcatgttcac cataacgtcc actgtaatat acatttgata atactgtttc
     4141 ttgagattta tcctcaagat tttccacaag gagttttaac tcttcctcca tggctgcaat
     4201 aattccaatt ttcatgatat ttccttataa tttaaaaatt gcataaaaca agagagccaa
     4261 caaaataagc aaaatgaata aaactaaatt caacttacgt tgaaattcat ttcgttttgc
     4321 attctcaatc cggcgactct tgtagatatg tttaccgttc tcgtcagtta gttgctccat
     4381 gccatcttct gtagaaataa ttttagtttc ttgatctaag attgtttgac ctgaaacttt
     4441 ttcacctcga tttgaacgtt caatcatatc atctgttaac aatggttttc ccattaagaa
     4501 tgacctccca tttttaaacc gtaatattgt aaagcaatga tcgttttcgc atcttgaatc
     4561 atgccttttt ctaccatttg catacaatcc tcataactta actctagaag ctcaaggacc
     4621 tcatcatcat cttgtggcct aggattttca actttttgta aatcagtagc tagatagagg
     4681 acgattttct cattacaaaa accaatagca gtgtagaaac tataaagaat ttcaagattc
     4741 cctgtgtagc cagtctcttc ttctaattcc cgaagggcag cttcttcctt agaacctgac
     4801 tcacccgtct caagtttccc tgctggaatt tcgtaagaaa taccttcaat agccttacga
     4861 tactgtttca ctaaaacaat tttatgctca ggagttaccg ccaaagtagc aacagctcct
     4921 ccatgaaaaa caagttcacg tttggattgt cccaatccat ttggcaactc tacatcatca
     4981 acagctactt tgataatttg tccatcaaaa acagtttgac gattgattgt tttttcttca
     5041 aaatccataa gaacctccca aataagtgtt acttttgtga aggatgatgt ggttttttat
     5101 tggcatatcc ttctttattg acttgacgtc ctcttccaat agctatgcta tcaattggaa
     5161 cattatctgt gatggtagat ccagctgctg ttaaagcatt atcaccaatt tcaagtggtg
     5221 caatcagagt agaattactt ccgataaaga cattacttcc gatttctgtt ttaaatttat
     5281 tttgtccatc ataattgaca gtaatcgtcc ctgcgccaaa gttaacatca caacctacct
     5341 ctgcattgcc gatataagtt aaatgacctg ccttcgtatt ctctcctatt tgagaaccct
     5401 ttacttcaac gaaattacca atatgaacac cttttgcaag acttgttcct ggtctaatat
     5461 gcgcataagg gcctacggtt actccatcag aaataatgga ttcttcaacc atcgaattag
     5521 tgatggtaac atcatttccg acttgagcat ctactaaata actaccattg gttaataaag
     5581 tacctttacc aatcttcgtc tgtcctttta aagtcacatt gggctcaatt acagattctt
     5641 ctccaatctc aacatcaata tcaatataag cggaatcagg attaaccact gtgaccccat
     5701 taaccatatg ttgacgtgca atgcgctgac gcatcacttt ttcagcagtt gcaagtgcta
     5761 cacgatcatt aactcctaga ctttcgtcaa agtcacgaag tttataggca cctacctttt
     5821 tacccgcttc tttgaaaatt ccaataacat cagttagata atactctcct tgagcgttat
     5881 ttgtattaat gtctttaagt gcttcaaata gactttgatt atcaaaaaca taagttccag
     5941 tattaatttc tttaacctgc tgctcaaagt cattagcatc cttttgttct acaattttag
     6001 taacttcatc atctgaatta cggataatac gaccatatcc aaatggattt gcagcatcgg
     6061 ctgttaagat tgttgcaacg tttttatgat tgacatgaaa atcaattaag ttttttaaac
     6121 tttctccagt aatcaggggt gtatcacctg caataactag tgtatgtcct tttgatgtcg
     6181 ctaattcttc ttccgccatc attacagcat ggccagttcc tagctgttct gtttgcatta
     6241 caaactctga tttgtcacct aaaacatcac gtacaagctc cgccttatgt ccaataacag
     6301 taacaatttt acttggctca atagcttgca cactacggaa aacatgctca agcatggtaa
     6361 taccagatac tttatgcata acttttggta aatcagattt catgcgagtt cccttacccg
     6421 cagctaaaat aatggcataa tttgacatat taattcctct atctctgaat ataattcaac
     6481 ctattatatc ataatgcctt caacttatca ctaatacccc tttaaatccc tattacaata
     6541 gactttagct aaaatagctt acttcaatta ggaataaaat agctaccttt tggtagctat
     6601 tcaatacatg aaatccgtta attttttctt ctctgtttgc aatcagtatt tttataaatc
     6661 tatcattgca atgctcagat ggtaaaaacg attagtttaa ctctgttgaa atttgccaca
     6721 tcacaccaaa ttggtccact aagtttgcgt aagcaggact ccagaaagtt tcttgtaact
     6781 ctaactgaac ttcttgtgca ctcttcttta atttttcata aatttcacgc gcctcctcta
     6841 cgctacccac aataatagcg gctgtaacat ttttaccagc ttgatacacg aaatctggat
     6901 tttcatccga gatttgaaga cgaatgccat caaaaataag ctgagcatta atgattaaat
     6961 ccttgcggtc ttcaagacag ttggggtcca tctcttccca tgtcatcata ttcactaaat
     7021 ccgcttgaaa gacatcttta taaaaatcaa ccgcttgtcg cccatttcca tttgttacga
     7081 tgtatgtttc tagtgcttta accataagtt ctccttttta ttttacttca cggattgcaa
     7141 agtaattatt gtcattatcg gaaaaattaa aaactctcat actccccata tcaacaatag
     7201 gatttgtcat aacctcattt gctgttaatt cttgatatgt actgtcaata tcagtagttt
     7261 caaataaaat agagggagtt gctaaatcta gctcaggact catttgagct ataattgctt
     7321 tgtcatgtaa gacgaagcta acttcactat ccaacttcgg tgcaactata taggttacat
     7381 aatctccttg tgtctgtttt tcaacacgct caaaacccac cttgtttttc caaaaatctg
     7441 ctgatgcttc aacattgcta acgtagagca ttacctgtcc aattgatgaa atcattttat
     7501 tatctcactt tcggttttat tactaattag tataacatac agtatttaaa ataagtaaaa
     7561 aatgactcaa aagagtcatt tacttatttc gttaccatat ctagaacttt catcaccgtt
     7621 ctactatgtt ctaaagcttg agcagcacgc ttaaaatcta aattgctaat aataccttca
     7681 aaaataacaa attcatcgtg catgcggtgc ctatcaccat ttaattgata aacatgactt
     7741 tcttgtccat tcatagttaa tgaaatttta ggcatagtat tagttggccc taaaatcgct
     7801 atagacccct tatcaccttg aatagtagat tttacttctg cactacaatc ttttgctcca
     7861 atacagactg ttttaaagtg cccatagtct aggactagaa ctcctgaagt atcaatacca
     7921 cgctctatat ttggtaaata ctgtgctgtt ataggttctc caaaaagtcc aattactaaa
     7981 tgtaaattat aaatattaag gtctctcaac gcacctccac ccatttcagg attgaaagct
     8041 ggtgctattt ccccacgttt aaaggcatca taacgcgatg aatattggga ataattacat
     8101 tctacaattt taatatctcc aaggttgctt aaatgttctt ttacaaggtc aaaattaggt
     8161 aaatattgat ttgtaattgc ttcgagtaat attagttgcc tagtattggc aatagataca
     8221 agttcttcta attgacttgc ttctaatgta aatggcttct cacaaatgac atgttttcct
     8281 gccaagagtg cctccttagc atagtcaaaa tgcaagtgat tcggcagtcc aatatagact
     8341 gtgtcaatct cctcattgtc caagacagcc ttatattcac tcgtagctag agacatgtta
     8401 tattccttag ctagatcttt agctgtctcc aaacttctaa ctgttgataa gatagcaact
     8461 agatcaatac cttcaatttt ctgcaagaca ggcaaaactt ccttaacaat catacctgtc
     8521 cccaaaaccg ctaatttcat tcgttaatct cctttgggta aaccacttgg aattcctcta
     8581 aaactatcct agagtcctca ctaaacatta aaccaaactc ctcaaaataa ggtttaaaga
     8641 aatcaatgtg agcttttctc caccattcta aagttttatc tccttctcct tctttaaaag
     8701 catggtctgc agagacatct ttaaaaggga caatatcaac tttagttatt tcaataatac
     8761 agatagcttc atcattgcta tccaaaataa tatcatatga accaacttgt gtaacccttc
     8821 ctcatctaat tcatataaat cataggcaga ggccgttgca gtttttgtcc catctacaac
     8881 taactttgcc agaagacctg gttctgctcc aaattgccat gcgtctatct catcaccgat
     8941 tgaaggattc aaagatttaa acttattcca taattcttgt gccttcatat tacctccgaa
     9001 gcaattccca caacaaactt aactgcgtta ctcatttttc tttgtctttt aaatccttct
     9061 ttaacatcta aaatataaac agtttgacca atccaaacac gtaaataaac agattttatt
     9121 aaaatatcac ctgatattcc tttaacttcc cgctcagtac catcagatgc ttcaaattcc
     9181 gtacgtaata accaagtatt accacaacca aattcaatct attttctcat ttcaaactcc
     9241 agccaccatc aattttgaca atttcacctt gcattgatct cgcttttcct gaagcaagaa
     9301 atccagttaa ttcagcaact tcacttggtt tagtccaacg accaattggc gtctcactag
     9361 ctacccattc tgctaatcca ccaggttcaa aatcactagc agtcatagca gtttgtacag
     9421 cacctggcgc tataccaaaa atttgaatac aatcttttgc gtaatccaaa gccaattgac
     9481 gtgtgaatcc cgcaagggca tgtttagaac tagtatatgc agctccaccg ccacccgcta
     9541 taaaacttgc tattgagcac atattgataa taatacctga cttcttttcc accatacgtc
     9601 taagataatg acgtgttagc ctaacagtca cgaaaaaatt aatatcaaat aggtgttcca
     9661 gttcttcatc acttacttct agaagtggct tgtaggcatc tagaatacct gcagtattac
     9721 acaaaatatc aacagtcggt accattgtga atagtgggga taaatcagag gataaatcca
     9781 actttataaa gttaaaatta ccatttaagt tcggtttatc cgacttatcc acaccatata
     9841 cttgataccc ttgtttcaaa aaatattgag cctgtgcata accaattcca ctagcacagc
     9901 ccgtcactaa aacaacttta gtcattgacc tcgatccaat cctctgctaa aacatcacat
     9961 ggtgttggtg cccacatgga aaaaccttca ccttcaccag taacattaat aagaaaataa
    10021 ggtgtagcct gtagtacttt cccattaact tcaagggtat caaataactg aacataattt
    10081 tcagcgcccc cccaacctgt acgtacatat tttttctttg cttttaaacc tggtaaaatt
    10141 tcttcaaatg tcatattgtt ctcctttatt gtctttaacc attgatatat caaggtttat
    10201 tgcttattta aaatcataat ttaaagtttc ttcgcctttt agttcatcta aaggctacta
    10261 ttgttttcat aggataaaat agtttctggt catccattat tagtaaataa tatttcgtct
    10321 ttttcttgtt ggtcaagaac attatttttg ttcttactcc ttcaattccc acaataaagt
    10381 tatctgcttg ctccgtcaac aaaacaggtc cattttccac taattcataa gaaatactta
    10441 gttgatttag cacagctata actttttcaa aatcattcgt aattttctcc attcttccat
    10501 agagttagca taactatttg tagactagag aacctaaaat tgatacactg tttttcactc
    10561 aaaaaatttt accttcataa tcttcattat acctataaca attttctttt acaaatttgt
    10621 tccccttaaa ggaaattgtt tttcaaaata ggatgtttat actattgtat tatcggatgc
    10681 tttatctcgc tagtgcaaaa tcttctcata acacatttct tccttttcaa aataagtttt
    10741 tgatttaccc ttaaaagaaa aaccaaacct atcgtaaaat ttccttgcgc gtgtattttc
    10801 tgtaaatacc caaagtatca ctttattata acctgcttgt tgtatctctg atagtacatg
    10861 attcatcatt tgagacccat aaccttttcc ccaaccttct ttaagactat gtatacaaat
    10921 gagttctgca taatcaagca taccatcttc tctactttta tcccaccaag ccatacaatg
    10981 aggattgcta tctacttcca atatataccc cttaccaacc tccttgtgta gtagctgttg
    11041 atacattgtt atggcaggtt ctatttccgt agttttttga atgatatcct caggtagtat
    11101 cttaccaaaa gcagctttcc agctctccgt ttgaatataa gctaaattga cttcatctcc
    11161 taatcttgct ctcctgatta tgcaagccat aactaaaatc ctttcaaaaa ttattattaa
    11221 ctgcctgata actactccaa ttataccatt ttcgtgatat taatttttat tttgaacagt
    11281 tctggttctc atttatcatt atatttctaa aattttagat ggttttaaac attaacctca
    11341 gattaggcca attattgata atca
//
LOCUS       DBJYSH010000066        11226 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.066, whole genome shotgun sequence.
ACCESSION   DBJYSH010000066 DBJYSH010000000
VERSION     DBJYSH010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 11226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11226
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.066"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            42..1022
                     /locus_tag="ACUBV3_001347"
     CDS             42..1022
                     /locus_tag="ACUBV3_001347"
                     /EC_number="1.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002828835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HHU3847846.1"
                     /translation="MGGCNMTIKNLTVAGSGVLGSQIAFQAAYKGMSVTIYDINDEAL
                     NKGKERIKKLAKVYQSEIETAKEAYSDKAKSIKYNKNLLPSLDHIFLSKVADSLDLIA
                     DLPNQITFSKNLDQAVSDADLVIEAVPETVSIKEDFYKQLAKVAPSKTIFATNSSTLV
                     PSQFADITGRPDKFLAMHFANNIWQNNIVEIMGHKGTDDEVVKEALAFSKDIGMVPLH
                     IHKEQPGYILNSILVPFLESALALYYNKVSDSETIDKTWKLGTGAPMGPLEILDIIGI
                     DTAYNIMKNYSDTNSDPNSLHAHLAKMLKEEFIDKGRTGKAAGHGFYDYD"
     gene            complement(1069..1617)
                     /locus_tag="ACUBV3_001348"
     CDS             complement(1069..1617)
                     /locus_tag="ACUBV3_001348"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HHU3847847.1"
                     /translation="MTKALISIDYTYDFVADDGKLTAGKPAQSIASAIADVTEKAYRS
                     GDYIFFAIDNHDIGDVFHPETNLFPEHNIKGTSGRNLYGPLGTLYETIKEDSRVFWID
                     KRHYSAFSGTDLDIRLRERRIDTLILTGVLTDICVLHTAIDAYNLGYKIEVPAAAVAS
                     LNDSNHQWALNHFKTVLGATIL"
     gene            complement(1684..2469)
                     /gene="codY"
                     /locus_tag="ACUBV3_001349"
     CDS             complement(1684..2469)
                     /gene="codY"
                     /locus_tag="ACUBV3_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001133183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HHU3847848.1"
                     /translation="MPNLLEKTRKITSILQRSVDSLDAELPYNTMAAQLADIIDCNAC
                     IINGGGNLLGYAMKYKTNTDRVEEFFETKQFPDYYVKSASRVYDTEANLSVDNDLSIF
                     PVETKENFQDGITTIAPIYGGGMRLGTFIIWRNDKEFSDDDLILVEIASTVVGIQLLN
                     LQTENLEENIRKQTAVTMAINTLSYSEMKAVAAILGELDGLEGRLTASVIADRIGITR
                     SVIVNALRKLESAGIIESRSLGMKGTYLKVINEGIFDKLKEYN"
     gene            complement(2595..3806)
                     /locus_tag="ACUBV3_001350"
     CDS             complement(2595..3806)
                     /locus_tag="ACUBV3_001350"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HHU3847849.1"
                     /translation="MKIFDKSMKLEHVAYDIRGPVLEEADRMRANGEKILRLNTGNPA
                     AFGFEAPDEVIRDLITNARESEGYSDSKGIFSARKAVMQYYQLQNIHVDMDDIYIVNG
                     VSEGISMSMQALLDNDDEVLVPMPDYPLWTACVSLAGGNAVHYICDEEANWYPDIDDI
                     KSKITSKTKAIVLINPNNPTGAVYPREILQEIVDIARQNDLIIFSDEVYDRLVMDGME
                     HIPIASIAEDIFTVTLSGLSKSHRICGFRVGWMVLSGPRQHVKGYIEGLNMLANMRLC
                     SNVLAQQVIQTSLGGQQSIDSMLLPGGRIYEQRNYIHKAINEIPGLSAVKPNAGLYLF
                     PKIDTDMYRIDNDEEFVLNFLKQEKVLLTHGRGFNMNTADHFRIVYLPRVDELTELQE
                     KMARFLSQYKR"
     gene            4074..4526
                     /locus_tag="ACUBV3_001351"
     CDS             4074..4526
                     /locus_tag="ACUBV3_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HHU3847850.1"
                     /translation="MSQKYERILVAIDGSTESELAFEKAVNVALRNDSELILTHVIDT
                     RALQSFATFDTYIYEKLEKEAKDVLEEYEKQAREKGADKVRQVIEFGNPKTLLAHDIP
                     EKEKVDLIMVGATGLNTFERFLIGSSSEYILRHAKVDLLIVRDPNKTM"
     gene            complement(4582..5964)
                     /locus_tag="ACUBV3_001352"
     CDS             complement(4582..5964)
                     /locus_tag="ACUBV3_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HHU3847851.1"
                     /translation="MAIKAVFFDIDGTLLNDRKNVQKSTIKAIRNLKDQGILVGLATG
                     RGPSFVQPFLENLGLDFAVTYNGQYIYSRSEIIYTNQLSKTTVYRLIRYAGARRREIS
                     LGTASGLLGSGIIGLGTSRLGQIVSSLVPRKWAKAIERSFKHFIRRIKPQNIDSLMII
                     LREPIYQVVLVATEGESERIQKQFPRVKLTRSSPYSMDVISEEQSKVKGIERVGQRYG
                     FDLSEVIAFGDSDNDIEMLSQVGIGVAMGNASQQVKENARYTTADNNDDGISKALAHY
                     GLIQFEIEKTFSSRDENFNKVKSFHLLMDGETIETPRLYDSKEAGFRSDFKVEEIVEF
                     LYAASQGNQKVFDQSIRNLHLAIDKARDKVISKDHPETPLVGEVDALTDLLYLTYGSF
                     VLMGVDPKPLFDTVHEANMGKIFPDGKAHFDPVTHKILKPDDWEEHFAPEPSIRRELD
                     SQIQKSLNRK"
     gene            6033..6995
                     /locus_tag="ACUBV3_001353"
     CDS             6033..6995
                     /locus_tag="ACUBV3_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HHU3847852.1"
                     /translation="MKKILVLHTGGTISMNANEKGQVMSSADNPMKYVDLSLDDLDLT
                     VVDFLNLPSPQITPHHMLDIYHYLKQHASNFDGVVITHGTDTLEETAYFLDTMILPKI
                     PIIITGAMRSTNELGSDGVYNYLSALRVANSTKAADKGVLVVMNDEIHAAKYVTKTHT
                     TNVSTFQTPTHGPLGIIMKQDLLFFKATEERVRFDLDKITGTVPIVKAYAGMGDSGII
                     SFLNSQNISGLVIEALGAGNMPPKAAQEIEELIEQGVPVVLVSRCFNGIAEPVYGYEG
                     GGARLQESGVMFVKELNAPKARLKLLIALNAGLTGQNLKDYIEG"
     gene            complement(7030..7908)
                     /gene="aroE"
                     /locus_tag="ACUBV3_001354"
     CDS             complement(7030..7908)
                     /gene="aroE"
                     /locus_tag="ACUBV3_001354"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HHU3847853.1"
                     /translation="MNSLNGETLLTGLIANPARHSLSPLMWNTSFQEKNMNYAYLTFE
                     VEEGKLTEAVRGVRALGIRGVNVSMPFKQSVIPLLDDLSPQAKLVGAVNTIVNQGGTG
                     RLVGHMTDGIGCFKALAAQGFSAKNKIVTIAGIGGSGKAVAVQAAMEGVAEIRLFNRN
                     SSNYDKVIDLSDKIKKQFQIKVVVDYLENKTAFKDAIRTSHFYIDATSLGMRPLDNYS
                     LINDPEILTPNLVVVDLVYKPKETALLRFVRQNGVKHAYNGLGMLIYQGAEAFQLITN
                     QEMPISSVERVLQTEN"
     gene            complement(8006..8920)
                     /locus_tag="ACUBV3_001355"
     CDS             complement(8006..8920)
                     /locus_tag="ACUBV3_001355"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HHU3847854.1"
                     /translation="MLQNIGQTGIQATRIALGCMRMSDLEGKQAEEVVGTALDLGINF
                     FDHADIYGGGLSELRFRDAIKHLNVNRDKMIIQSKCGIREGYFDFSKEYILSSVDGIL
                     ERLGTEYLDFLILHRPDVLVEPEEVAEAFTKLRAEGKVKHFGVSNQNRFQMELLQSYL
                     DEPLAVNQLQLSPAHTPMFDAGLNVNMLNKASIEHDDGIVDYCRLKRVTIQAWSPFQI
                     DLSRGLFVNHPDYKELNETIAKLAKNYNVSSEAIVIAWILRHPAQIQAIVGSMNPSRL
                     KAIDKANDIALTREEWYDIYRSAGNILP"
     gene            complement(9211..>11226)
                     /gene="recG"
                     /locus_tag="ACUBV3_001356"
     CDS             complement(9211..>11226)
                     /gene="recG"
                     /locus_tag="ACUBV3_001356"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000926642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HHU3847855.1"
                     /translation="MLLQSPISNLKGFGPKSAEKFQKLDIYTVEDLLLYYPFRYEDFK
                     SKSVFDLVDGEKAVITGLVVTPANVQYYGFKRNRLSFKLRQGEAVLNVSFFNQPYLAD
                     KIELGQEVAVFGKWDATKSAITGMKVLAQVEDDMQPVYRVAQGISQSTLIKAIKSAFE
                     ISAHLELKENLPATLLEKYRLMGRSQACLAMHFPKDITEYKQALRRIKFEELFYFQMN
                     LQVLKSENKSETNGLPILYSKHAMETKISSLPFILTNAQKRSLDEILSDMSSGAHMNR
                     LLQGDVGSGKTVIAGLSMYAAYTAGFQSALMVPTEILAEQHYISLQELFPDLSIAILT
                     SGMKAAVKRTVLAAIANGSVDMIVGTHALIQDSVQYHKLGLVITDEQHRFGVKQRRIF
                     REKGENPDVLMMTATPIPRTLAITAFGEMDVSIIDELPAGRKPIITRWVKHEQLGTVL
                     EWVKGELQKDAQVYVISPLIEESEALDLKNAVALHAELSTYFEGIAKVALVHGRMKND
                     EKDAIMQDFKDKKSHILVSTTVIEVGVNVPNATIMIIMDADRFGLSQLHQLRGRVGRG
                     YKQSYAVLVANPKTDSGKKRMTIMTETTDGFVLAESDLKMRGSGEIFGTRQSGIPEFQ
                     VADIVEDYPILEEARRVASDIVKDNNWKENTEWALILDNLRQHSDFD"
BASE COUNT         3642 a         2203 c         1784 g         3597 t
ORIGIN      
        1 cttttgttaa actttcttcg aaaacgtttt cgaatataag tatcggaggt tgtaatatga
       61 caattaaaaa tctcactgtt gcaggaagtg gcgttctagg tagtcaaatc gcctttcaag
      121 cagcttacaa aggaatgtct gtcaccatct atgacattaa cgatgaagct ttaaataagg
      181 gaaaagaacg tatcaaaaaa ttagctaaag tctatcaatc agaaattgaa acagcaaaag
      241 aagcatatag tgataaagct aaaagtatca agtataataa aaacttattg cctagcttag
      301 accatatttt tctttcaaaa gttgccgatt cattagactt gattgctgat ttaccaaacc
      361 aaattacttt ttcaaaaaac ttagaccaag ctgtaagtga cgccgattta gtaatcgaag
      421 ctgtcccaga aacagttagt ataaaagaag atttttacaa acaattagct aaagtggcac
      481 cttcaaagac aatctttgca acaaattctt caacacttgt tccaagtcag tttgctgata
      541 taacaggacg gcctgataaa tttttagcaa tgcatttcgc taacaatatt tggcaaaata
      601 atattgttga aattatggga cataagggaa ctgacgacga ggttgtcaaa gaagctttgg
      661 cattctcaaa agatattggt atggtacctt tacatattca taaagaacaa cctggctata
      721 ttcttaattc aatactagtt ccattcttag agagtgcatt agcattgtac tataataagg
      781 tttctgattc tgaaactatt gataaaacct ggaagttagg aacaggagca ccgatgggac
      841 ctttagaaat tttagatatt attggcatcg ataccgctta taatatcatg aagaattatt
      901 cagataccaa cagcgatcct aatagtcttc atgctcacct tgcaaaaatg cttaaagaag
      961 aattcattga taaaggacgt accggtaaag ctgctggaca cggcttttat gattacgact
     1021 aaactataaa agaggttaga taaaaatcta acctctttta taattcaact aaagaatagt
     1081 tgcaccaaga acagttttaa aatgatttaa agcccattga tgattactat catttaagct
     1141 agctacagct gctgctggga cctcaatttt ataacctaag ttataagcat caattgctgt
     1201 gtgtaataca caaatatctg tcaatactcc tgtcaatatc aaagtatcta ttcgtctttc
     1261 acgtaggcga atatctaaat cagtgccaga aaaggcagaa taatggcgct tatctatcca
     1321 aaaaacacga ctatcttcct taatagtttc atatagagtt cccaaaggac catataggtt
     1381 tctaccagaa gtccctttaa tattatgctc aggaaaaaga ttggtctctg gatgaaaaac
     1441 atctccaata tcatgattat caatcgcaaa aaaaatataa tcccctgacc ggtatgcttt
     1501 ctctgtaacg tcagctatag ctgaagcgat agattgcgct ggtttacctg ccgttaattt
     1561 cccatcatct gccacaaaat catatgtgta gtctattgag atgagtgctt ttgtcatttt
     1621 tcttcttatc tcctcttgat ttcaaaaaag gaactgaaag ctactcccag tcccatgcta
     1681 tgattaatta tattctttta atttgtcaaa aataccttcg ttaataactt tgaggtatgt
     1741 tcccttcata ccgagtgagc ggctctcaat gattcccgca gactccaatt tacgaagagc
     1801 atttacaata acggaacgtg taattccaat acggtctgca ataacagatg ccgttaggcg
     1861 tccttcaagt ccatctaatt ctcctaagat agcagctaca gctttcattt ctgaataaga
     1921 tagagtgttg atagccattg tcaccgctgt ttgtttacga atattttctt ctagattttc
     1981 tgtttgaagg ttaagtaatt gaataccaac aacggtgctt gcaatttcaa ctaaaatcaa
     2041 atcatcgtcg ctaaattctt tatcattacg ccagataata aatgttccca agcgcattcc
     2101 accaccataa attggagcga ttgttgtaat accatcttgg aagttttcct ttgtctcaac
     2161 tgggaaaatt gacaaatcat tatctactga taagttagct tctgtatcat aaacacgact
     2221 agcagattta acatagtaat ctgggaattg ttttgtttca aaaaattctt ccacacggtc
     2281 agtgtttgtt ttatatttca tggcatagcc aagtaagtta ccaccaccat taataataca
     2341 tgcgttacag tcaataatat cagctagttg agccgccatt gtattatatg gtaattcggc
     2401 atctaagcta tctacggaac gctgtaagat ggatgtaatt tttctagttt tttctaataa
     2461 attcggcatt tccttcatcc tttgtatgtt ttgcgttaca attatatcaa agcattttag
     2521 cttttgcaaa tcgatatcag tacattttaa gaacttctat tattaattta atgtttatct
     2581 aataaaaatt cattctagcg cttgtattgg cttaaaaagc gagccatttt ttcttgtagt
     2641 tcagtcaatt catcaacacg tggtaagtaa acaattctaa aatggtcagc ggtattcata
     2701 ttaaatcctc taccgtgtgt tagtaatact ttttcttgtt ttaagaaatt aagcacaaac
     2761 tcttcatcat tatcaatacg gtacatatct gtatcaattt tagggaaaag atacagtcca
     2821 gcatttggtt ttacagcaga taaaccaggt atttcattaa ttgctttatg gatataatta
     2881 cgttgctcgt agatacgacc tcctggcaac aacatactat caatagattg ctgtccgcct
     2941 aacgatgttt gaataacctg ttgagcaaga acatttgaac acagcctcat gttagctaac
     3001 atattcaaac cttcaatata acctttaaca tgttgtcgcg gaccagacaa taccatccaa
     3061 ccaacgcgga aaccacagat gcgatgtgat ttagataagc cagataaagt aactgtaaaa
     3121 atatcctctg caatagaagc aattgggata tgctccattc catccataac taaacggtca
     3181 taaacctcgt ctgaaaaaat aatcaagtca ttttgtctag caatatccac aatctcttgc
     3241 aaaatttcac gcggatagac tgctccagtt ggattatttg gattaatgag gacaattgct
     3301 tttgttttgg atgtgatttt tgatttgata tcatcaatat caggatacca attagcttcc
     3361 tcatcacaaa tataatgcac agcatttcca ccagcaaggc taacacaagc tgtccataac
     3421 gggtaatctg gcattggaac tagaacctcg tcatcattat caagcaaagc ttgcattgac
     3481 atagagattc cctcactaac accattaaca atgtatatat catccatgtc aacatggatg
     3541 ttttgtaact ggtaatactg cataacagcc ttacgagctg aaaaaatacc tttcgaatca
     3601 gagtaacctt ctgattcacg cgcattcgta attaaatcac gaatgacttc atctggcgct
     3661 tcgaagccaa aggcagctgg atttccagtg tttaaccgca atatcttttc accatttgcc
     3721 ctcatgcggt ccgcctcttc taaaacaggg cctctaatat cataagccac atgttccagc
     3781 ttcattgatt tatcaaatat cttcataaaa atacctcgta tcttccatta taaaacaaat
     3841 attctcaaaa ttgaaacatt tactgcggtt tatcaagcga ttaagttaat attaagctat
     3901 ttcagaaaac taacataaaa cgctttcatt tttctagaaa aaacgttata atatagctat
     3961 aaaatgaata accaacatat ctaacttact catcactcac tccttctgat aagatggcta
     4021 gttgagtaca agaggttatc tctttttggt tatatttaga ggaggacagg cttatgtcac
     4081 aaaaatatga acgcatcctt gtcgctattg atggatcaac agaatctgaa cttgcttttg
     4141 aaaaggcagt taacgttgct cttcgcaatg attctgaatt aatcttgaca catgttatag
     4201 atacacgtgc tttacaaagc tttgcgacat ttgatactta tatctatgaa aaattagaaa
     4261 aagaagcaaa agatgtcctt gaggaatatg aaaaacaagc tcgtgaaaaa ggagcagata
     4321 aagttagaca agttattgaa tttggtaatc ccaaaacatt acttgcacat gatattccag
     4381 aaaaagaaaa agtagacttg attatggttg gtgcaacagg tcttaatacc tttgagcgtt
     4441 tccttatcgg atcttcctca gaatacatcc ttcgtcacgc taaagtagat ttactcattg
     4501 tacgtgatcc taataaaaca atgtaaattt aaatacgctc actttctaaa atatcctgag
     4561 ttatattaac tcaggatatt tttattttcg atttaaggat ttctgaattt ggctatctaa
     4621 ttcacgtcga attgatggct caggagcgaa atgttcttcc cagtcgtctg gttttaaaat
     4681 tttatgagta acaggatcaa aatgagcttt gccatctgga aagattttac ccatattggc
     4741 ctcatgtact gtatcaaaaa gaggttttgg gtcgactccc ataagaacaa aggagccata
     4801 agtcagataa agtaaatctg ttaaggcatc cacttctccc actaatggtg tttctggatg
     4861 gtctttagaa ataaccttat ctcttgcttt atcaatagct aagtgtaaat tacggataga
     4921 ttggtcaaat actttttggt taccttgact agcagcatac aagaactcaa cgatttcttc
     4981 tactttaaag tctgacctga aaccagcttc cttactgtca tataagcgtg gcgtttcaat
     5041 agtttcacca tccattaata gatggaagga ttttacttta ttgaaattct cgtcacgact
     5101 actgaatgtt ttttcaatct caaattgaat aagtccataa tgggctaatg ccttagagat
     5161 accatcatca ttattgtcag cagttgtata acgtgcattt tctttcactt gctgactggc
     5221 gttccccatg gcaacaccaa tgccaacttg agataacatc tcaatatcat tatcagaatc
     5281 tccaaatgct atcacttcgg ataaatcaaa accatagcgt tgaccaacac gttcaattcc
     5341 cttaactttt gactgctctt cagaaatgac atccattgag taaggactgc ttcttgttaa
     5401 tttaacacga ggaaattgtt tttgaattcg ctctgattcg ccctctgttg caactaaaac
     5461 gacctgataa ataggttctc gtaagataat catgaggcta tcaatatttt gaggtttaat
     5521 ccgacgaata aaatgcttaa agcttcgttc aatcgctttt gcccattttc tcggaacaag
     5581 gctagataca atctgcccca aacggctagt tcctagacca ataatacctg aaccgagtaa
     5641 tcccgaggct gttcctaatg aaatttctct tcttcttgct ccagcatatc gaatcagacg
     5701 atagacagtt gtcttagata attgattggt ataaataatt tcacttctac tatagatata
     5761 ttgaccatta taggttacag caaaatctaa accaaggttt tctaaaaaag gttgtacaaa
     5821 actaggacct cgccccgttg ctaaaccgac tagtattcct tggtctttca aatttcgaat
     5881 tgctttaatt gttgactttt gtacattctt acgatcatta agaagtgtac catcaatatc
     5941 aaaaaatact gccttaattg ccactgggtc aacatcccct cttttttatg ataaaataaa
     6001 ttatatcaca acttgaaaga agtataaaat caatgaaaaa aatccttgtt ctacatacag
     6061 gtggaacgat ttccatgaat gctaacgaga agggacaggt catgtccagc gccgataacc
     6121 caatgaaata cgttgattta tcattagatg accttgattt gactgttgta gattttctaa
     6181 atcttcccag tcctcaaatc acccctcatc atatgcttga tatctatcac tatctgaaac
     6241 aacatgccag taattttgat ggtgttgtca ttacgcatgg gactgacact ttagaagaaa
     6301 cagcatattt tttagacaca atgatactcc ctaaaatccc aatcatcatt actggtgcta
     6361 tgagaagcac aaatgaactt ggtagcgatg gcgtttataa ttacctatca gccctccgtg
     6421 tcgctaatag tactaaagct gccgacaagg gggtgctagt tgtcatgaat gatgagatac
     6481 atgctgctaa atatgtcaca aaaacacata caacaaacgt ttcgaccttc caaaccccta
     6541 cacatgggcc ccttgggatt attatgaaac aagacctgct tttctttaaa gcaactgaag
     6601 agagagttcg ttttgattta gataaaatta caggaactgt tcctatcgtg aaagcatatg
     6661 ctggcatggg tgatagtggt atcattagct ttttaaattc tcaaaatatt agcggattag
     6721 ttattgaagc actcggtgca gggaatatgc ccccaaaagc tgcacaagaa atcgaagaat
     6781 taattgagca aggtgtccct gttgttctag tgtctcgctg ttttaatggt attgctgagc
     6841 cagtttacgg atatgaaggt ggcggagcaa gattacaaga atctggtgtc atgtttgtta
     6901 aagaactaaa cgctcccaaa gccagactca aattactcat tgcactcaat gcaggtctca
     6961 cagggcaaaa tctaaaagac tatattgaag gttgataaaa aactctagaa atatttctag
     7021 agttttttat taattttcag tttgtaatac tctctcaaca ctgctaatag gcatttcttg
     7081 attcgttatt aattgaaatg cttctgctcc ttgataaatc agcatcccta gaccattata
     7141 agcatgtttc actccatttt gtctaacaaa tcgtaacaat gctgtttctt taggcttgta
     7201 aaccaagtcg acaactacta aattgggtgt taaaatttct ggatcgttaa ttaaactata
     7261 attatctaat ggcctcattc ctaaactagt agcatcaata taaaaatgac tagttctaat
     7321 agcgtcttta aatgctgtct tattttctag ataatcaacg actaccttta tttgaaactg
     7381 ttttttaatt ttatctgata agtcaatgac cttatcgtaa tttgagctat tacgattaaa
     7441 taatctaatt tccgcaactc cctccatagc tgcttgaact gcaactgctt tacctgaacc
     7501 accaatacca gctattgtaa ctattttatt tttagcactg aaaccttgag ctgctaaagc
     7561 tttaaaacaa ccaatgccat ctgtcatatg gcctactaaa cgtccggttc caccttgatt
     7621 aacgatagta tttacagcac ccactaattt agcttgagga gataaatcat ctagcaaagg
     7681 gataacactc tgtttaaatg gcattgaaac attaacacca cgaataccca atgccctgac
     7741 acctcgaaca gcttctgtta atttaccctc ttctacttca aatgtcagat aggcataatt
     7801 catgtttttt tcttgaaaag aggtattcca cattaacggg gatagagagt ggcgtgcagg
     7861 attggcaatt agaccagtta gtaaagtttc cccatttaat gaattcattg tataaccttc
     7921 tttcttaaca atttatttca gttcatttta ccaaaaactg actcaattaa aaagagagta
     7981 tgtaatttat actctctccc ttagtttaag gcaatatatt acctgcacta cggtaaatat
     8041 cataccattc ttcacgggtt aaagcgatat cattagcctt atcaattgcc tttaaacgag
     8101 atggattcat tgaaccaaca atcgcctgta tttgggcagg atgacgtaaa atccatgcaa
     8161 tcacaatagc ttcgctgcta acattgtagt tttttgccag tttagctatg gtttcattta
     8221 attccttata atccggatga ttaacaaata aaccacgaga taaatcaatt tgaaatggag
     8281 accaagcttg aatggtaact cttttgagac gacaataatc aacaatgcca tcatcatgtt
     8341 caattgaagc tttatttaac atgttaacat ttaaccctgc atcaaacatt ggtgtatgtg
     8401 ctggcgataa ttgaagttga ttcactgcta agggctcgtc aaggtaggat tgtaacaatt
     8461 ccatttgaaa ccggttttga ttgctaacac caaagtgttt tactttacct tctgccctta
     8521 gtttagtaaa tgcttcagct acttcttcag gctcaactaa tacatctgga cgatgaagaa
     8581 tgagaaaatc taaatattct gtccctaatc tttctaaaat accatctact gatgataaaa
     8641 tatactcttt agaaaaatca aagtaacctt ctcgtatccc acatttagat tgaataatca
     8701 ttttgtcacg attgacattt aaatgtttga ttgcatctcg aaagcgtaat tcagatagcc
     8761 ctcccccata aatatcagca tgatcaaaaa aattaatacc caaatctaat gctgttccaa
     8821 ctacttcttc agcttgtttt ccttccaagt cactcattct catacaacct aaagcaattc
     8881 gagttgcttg aatgcctgtt tgtccaatgt tttgtaacat atgcacccca ttctaatctg
     8941 tttgtattaa cagaaagatt aatcaattca ttataacaaa atttctatga aaaggcttac
     9001 tattacaaat gcaaaaagct gagtaaattt aactcaactt tttttctgat actcattatt
     9061 gactaaataa gtcggtaaga aaaatctatt ctgaaaagta ctattataaa ttacagtaat
     9121 cattattgtc ataacatacc tccacatctg tgataggaat atcatatgac agcaagctta
     9181 aactgagctt agcacaacct taatagaaac ttaatcaaaa tctgaatgtt ggcgtaaatt
     9241 atctagaata agtgcccatt ctgtattttc tttccaatta ttatctttga caatatcact
     9301 agcaacacgt ctggcttctt ctaaaatagg ataatcctca acgatatcag ctacttgaaa
     9361 ttctggaatt ccagactgac gagtaccaaa gatttcacca gaaccacgca tttttaaatc
     9421 cgactcagct aaaacgaaac catctgtcgt ttctgtcatg attgtcattc gttttttccc
     9481 agaatcagtt ttgggattag ccactaaaac agcgtatgat tgtttatatc cacgaccaac
     9541 acgcccacga agttgatgta gctggcttaa tccaaaacga tcggcatcca taataatcat
     9601 gatcgttgca tttgggacat ttacccctac ttcaataact gttgtagata ctaaaatatg
     9661 acttttttta tccttgaaat cttgcattat agcatctttt tcatcatttt tcatacgtcc
     9721 atgtacaaga gcaaccttag caattccttc aaaataagta gataattcag catgcaatgc
     9781 tactgcattc tttaaatcta aagcttctga ctcttcaatc aacggtgaaa tgacatacac
     9841 ttgagcatct ttttgcaatt caccttttac ccattccaac acagtaccta gctgctcgtg
     9901 tttcacccag cgtgtaataa taggtttacg accggcaggt aattcatcaa taatagaaac
     9961 atccatttct ccaaaagctg tgattgctag agttcgggga attggggtgg ctgtcatcat
    10021 taaaacatca ggattttctc ccttttcacg gaaaattcta cgctgtttaa caccaaaacg
    10081 atgttgctcg tctgtaatta caagccccag tttatggtac tgtaccgagt cctggataag
    10141 agcatgagtt cctacaatca tatcaaccga gccatttgca atagctgcta aaaccgtacg
    10201 cttaacagct gctttcatac ccgaagttaa tatagcgatt gataaatctg gaaataactc
    10261 ttgcagacta atgtagtgtt gttcagctag gatttccgtt ggaaccatca aagccgattg
    10321 aaaacctgct gtataagctg catacattga tagaccagca atgactgtct ttcctgatcc
    10381 tacatctcct tgcaataaac gattcatatg agctcccgat gacatatcag ataatatttc
    10441 atctaaagag cgcttttgag cattcgttag aataaaaggt aaagaggaaa tttttgtctc
    10501 catagcatgt ttactataaa gaataggcaa accatttgtt tcagatttat tttcggattt
    10561 caaaacttga aggttcattt gaaagtaaaa taattcttca aatttaatcc gacggagcgc
    10621 ttgcttatac tctgtgatat cttttgggaa atgcatagct aaacaagcct gactacgacc
    10681 catcaatcgg tatttttcca ataaagtagc tggtaaattt tccttcaatt ccaaatgcgc
    10741 actgatttca aaagctgact taatagcttt aatcaaagta gactgtgaaa ttccctgagc
    10801 tacgcgataa acaggttgca tgtcatcttc aacttgagct aaaaccttca tcccagtaat
    10861 agctgattta gttgcatccc atttaccaaa gacagctacc tcttgaccaa gttctatttt
    10921 atcagctaaa tagggttgat taaaaaaact aacatttaag acagcttccc cttgacgcaa
    10981 cttaaaactt aaacggttac gtttaaaacc ataatattgt acattagctg gagtaacgac
    11041 taggcctgta atgactgctt tttcaccatc cactaaatca aaaacagatt ttgatttaaa
    11101 atcttcatag cgaaacggat aataaagaag taaatcttct actgtataaa tatctaattt
    11161 ctgaaatttt tctgctgatt ttggtccaaa tcccttaaga ttagaaattg gtgattgtaa
    11221 tagcat
//
LOCUS       DBJYSH010000067        11006 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.067, whole genome shotgun sequence.
ACCESSION   DBJYSH010000067 DBJYSH010000000
VERSION     DBJYSH010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 11006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11006
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.067"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            85..1622
                     /locus_tag="ACUBV3_001357"
                     /pseudo
     CDS             85..1622
                     /locus_tag="ACUBV3_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043078.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            1799..3343
                     /locus_tag="ACUBV3_001358"
     CDS             1799..3343
                     /locus_tag="ACUBV3_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HHU3847856.1"
                     /translation="MTLQDEIKKRRTFAIISHPDAGKTTITEQLLYFGGEIREAGTVK
                     GKKSGTFAKSDWMDIEKQRGISVTSSVMQFDYAGKRVNILDTPGHEDFSEDTYRTLMA
                     VDAAVMVVDSAKGIEAQTKKLFEVVKHRNIPVFTFINKLDRDGREPLDLLEELEEVLG
                     IASYPMNWPIGMGKSFEGLYDLHNKRLELYKGDERFASIEDGDQLFANNPFYEQVKED
                     IELLQEAGNDFSEQAILDGDLTPVFFGSALTNFGVQTFLDTFLEFAPEPHGHKTTEGN
                     VIDPLAKDFSGFVFKIQANMDPRHRDRIAFVRIVSGEFECGMGVNLTRTGKGAKLSNV
                     TQFMAESRENVTNAVAGDIIGVYDTGTYQVGDTLTVGKNKFEFEPLPTFTPELFMKVS
                     AKNVMKQKSFHKGIEQLVQEGAIQLYKNYQTGEYMLGAVGQLQFEVFKHRMEGEYNAE
                     VVMTPMGKKTVRWINSDDLDERMSSSRNILAKDRFDQPVFLFENDFALRWFADKYPDV
                     KLEEKM"
     gene            3429..3809
                     /locus_tag="ACUBV3_001359"
     CDS             3429..3809
                     /locus_tag="ACUBV3_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HHU3847857.1"
                     /translation="MGIFSGLMGNASQVDTDKVENQLSDILIPGEQVDLAYTLIRDLI
                     VFTNYRLILVDKQGVTGKKVSYNSIPYASISRFTVETSGHFDLDAELKIWISSAIEPA
                     EVLQFKNDRNIVSIQKALAIAVLK"
     gene            4003..4737
                     /locus_tag="ACUBV3_001360"
     CDS             4003..4737
                     /locus_tag="ACUBV3_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HHU3847858.1"
                     /translation="MIIELKSVGKIFPKSQFRLDDISFNIAEGEIVGLIGSNGTGKST
                     ILKMINGLIPYDKGNIYYQGKEVKSFSDNKLRQMRKDIAYIFQNHNLLAGESVYYHLA
                     LVYKLNHQKVNHDAINDILDFLGLMDLKQVKCHSLSGGQQQKVAIAMAVLQKPKLILC
                     DDISSALDTNSEKEIFNLLSDLREKYGISILMIAHHLSLLKQYCDRVMILDHQTIVDT
                     VVPVKATLNQLKSNYVDQVKEFLLHD"
     gene            4730..5392
                     /locus_tag="ACUBV3_001361"
     CDS             4730..5392
                     /locus_tag="ACUBV3_001361"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HHU3847859.1"
                     /translation="MIDIIEKHGEGILKSFWETNLMLGLTLILCFLIAFPTGILLFSL
                     RKSYLIKHSLAYQLLNLFLGTLRSVPFLIFIFILIPLNRLIFGTSFGTIAAILPLTLV
                     SVSLYARYVEQALLNIPQVVVDRALSLGANKRQIIYYFLIPSIKIDLVLSFTATAISI
                     LGYSTIMGVIGAGGLGEYAYRFGYQEYDYPVMYLIVVLFIIYVFILQSLGYFIANRYS
                     RK"
     gene            5408..6238
                     /locus_tag="ACUBV3_001362"
     CDS             5408..6238
                     /locus_tag="ACUBV3_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HHU3847860.1"
                     /translation="MKKLLSLTCLIMMSLCLVACTKQAMSSKQIKDKNSKEKVITVAT
                     YSKPTSTFLDLIKDNVKEKGYTLKVVMVSDYIQANIALENKEHDANLLQHEFFMSIFN
                     KENDGHLVSITPIYHSLAGFYGQHLKNIAELKDGAKVAIPSDPANMTRALLLLQEKKL
                     ITLKNTSKKTKAIEDIITNPKKLRIEPVALLNLNQAYFEYDLVFNFPGYVTKINLVPK
                     RDRLLYEKKPDIRFAGALVAREDNKNSDKIKVLKEVLTSKEIRHYITKEIPSEADVAF
                     "
     gene            6473..8059
                     /locus_tag="ACUBV3_001363"
     CDS             6473..8059
                     /locus_tag="ACUBV3_001363"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HHU3847861.1"
                     /translation="MKFTELNLSQDILSAVEKAGFVEPSPIQEMTIPLALEGKDVIGQ
                     AQTGTGKTAAFGLPTLNKIHTEDNTIQALIIAPTRELAVQSQEELFRFGRDKGVKVRS
                     VYGGSSIEKQIKALRSGAHVVVGTPGRLLDLIKRKALKLNHIETLILDEADEMLNMGF
                     LEDIEAIISRVPEARQTLLFSATMPDPIKRIGVKFMKDPEHVKIKATELTNVNVDQYY
                     VRVKENEKFDTMTRLMDVDQPELSIVFGRTKRRVDELTRGLKLRGFRAEGIHGDLDQN
                     KRLRVIRDFKNDHIDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KSGQSITFVSPNEMGYLTIIENLTKKRMTGMKPATASEAFQAKKKVALKRIARDFEDQ
                     ELVSKFDKFKADALELATQYTPEELALYVLSLTVQDPESLPEVEITREKPLPFKPSGG
                     GFKGKGGRGNGRGGDRRRNDRGDRRGNRDRDDRGGRRDFKRRDDKFKKDNRRQENKKP
                     HKNTSSEKQTGFVIRNKGDK"
     gene            complement(8242..8508)
                     /locus_tag="ACUBV3_001364"
     CDS             complement(8242..8508)
                     /locus_tag="ACUBV3_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HHU3847862.1"
                     /translation="MIKVPAYMYVLECSDGTLYTGYTTDVKRRLNTHNTGKGAKYTRA
                     RLPVKLLYSEAFNSKQEAMRAEALFKQKTRKAKLTYIKQHKNEQ"
     gene            complement(8501..9265)
                     /locus_tag="ACUBV3_001365"
     CDS             complement(8501..9265)
                     /locus_tag="ACUBV3_001365"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HHU3847863.1"
                     /translation="MIDTILKENERIDQLFSTDVKIIQNKEVFSYSIDSVLLSRFPKL
                     PSRGLIVDLCSGNGAVGLFASTKTSATIIEIELQERLADMAKRSIKLNKLEKQVTMIN
                     DDLKNLLDHVQRSNVDLMLCNPPYFKASETSKKNLSPHYLLARHEITTNLREICQIAQ
                     HALKTKGRIAMVHRPDRFLEIIDTMRQFNLAPKRIQFVYPKLGKDANMLLIEAIKDGS
                     TEGMKILPPLVVHQDNGDYTETIFDIYFGENGKSHD"
     gene            9400..10140
                     /locus_tag="ACUBV3_001366"
     CDS             9400..10140
                     /locus_tag="ACUBV3_001366"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HHU3847864.1"
                     /translation="MFYTYLRTLVMFLIWVANGNAHYHNEDKMLKDDENYILVAPHRT
                     FWDPVYMAFAARPKQFVFMAKKELFTNRLFGWWIKMCGAFPIDREKPGQDAIRYPVKM
                     LKNSNRSLVMFPSGSRHSKDVKGGVAVIAKMAKVRIMPAAYRGPMVFKNLLKGHRVDM
                     NFGNPIDVSDIKRMDAEGIAEVSRRIQEEFDRLDRENETYDDGKKLNPLTYIYRLPLA
                     IIAIVLLVLTLIFSYLASFVWDPQKHLK"
     gene            10240..10893
                     /locus_tag="ACUBV3_001367"
     CDS             10240..10893
                     /locus_tag="ACUBV3_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HHU3847865.1"
                     /translation="MFEIVLEKIKSHKWETTGIIVGLLLFGILGLNHFGTHHKEDDLN
                     INLEKKVSTITEKKVPMISHVKDKVSNQVTVDVKGAVNHPGVYSLPSQSRVTDAIKRA
                     GGLSNLADSKSVNLAQKLQDETVIYVAQKGEKITVVEEEKANNIATQGNSKGKINLNK
                     ADLSSLQTISGVGAKRAQDILDYRDSQGGFKTIDDLKNVSGIGEKTLEKLRQDVTLD"
BASE COUNT         3642 a         1670 c         2150 g         3544 t
ORIGIN      
        1 ttaatctgct aagatatagt acgtaataaa aacttaaccg gttaattatt ttttgaaata
       61 tattttttct ggaggaaaat agtaatgaat aaatcattca ataccaaatt aggtttggta
      121 actgtagcgg cattatcagg tattgtatta acgtctcaat tacctgtgaa cgctaaagct
      181 gagacaccag taatggcagc aagtgcacaa caaggtttcc gttttgtagc aaccgttgta
      241 gatagtcaga cgcgtgtctt gccgggaaaa ttggtaactt tgtcagaggt gacaactggt
      301 cagcctaaaa ttattgcaag tgtaaaaacg aatgatgccg gacaagctat ttttaataat
      361 ctccctataa aacgtaacct tagtgtgtct gttgatggac aagtaaaagg atatactatt
      421 cgaactgacg tagcaggctc tagtaaagca gctagtttta cagcaacagg agtagggaca
      481 aatgagccaa cgtattcaaa aaaaaccata gacattacag tgagagacca aaatgctgaa
      541 cctgtttcag gcaggacagt taccttaaaa acacatgctg gtcgtgagat tgctagcttg
      601 gtttctggtg acaatggatt aactcgcttt actgatcgtt tactagatgg aacattctac
      661 caatattttg ttgatgggaa aaaattggtg atattgtacc tggagaatca agaagtgcat
      721 atgttaatgt tgcacctaaa aaagattatt ttacctttac agtcactgct ttagataaag
      781 atggacttgt tgttaaggga aaagaagtaa cgttgactga tattactgat ggtaaagcag
      841 tagcgcttgc ttctttaaaa acgaatgata atggacaagc tatctttacc aacctaccat
      901 tatcaagaaa tatcagtgtt tcaattgatg gaaaatcaaa gggctatact cttcgaactg
      961 atgtagcagg ctctcataaa gcggctgcat tctatgtaga tggtaaaggt accaaagcac
     1021 ctcaatttag tgcagaagca gcggttgtaa ctatttacga tgctaatgga aatactatag
     1081 caaaccaaga agtgacatta actaattcaa atgggacaat tgttgcaaaa ggtttgactg
     1141 aaaaagatgg caaagcaagg ttcgccaata aattaatggg aggaacgtta tacaatattt
     1201 ttgtaaatgg tattgagatg cctaaaactg cccttgttgg tagtgatgtc agtgtttttc
     1261 taacagataa acaaattaag aaagaaaaac ctgttactcc tgataaattg gataaagaaa
     1321 tgaaccaaga gaaggaaaag gagctcttag atactccaaa accagtacca cctacttcga
     1381 aaccaacagc accaattaaa aataaaaagg acgattcccc taagaagttt acgaaaagtc
     1441 aaccttctag tttgaagtca agtgttaagg ctataggtgc taagccaaaa gctatgacag
     1501 ccttagctaa aaaattgcct aaaactggtg atcaagccat tagtatttta actttggttg
     1561 gttttattgt aacagggtta gcgattatgt taggcttctt taaaaaatca cgatcaaaat
     1621 aatttatttt agaagcttgg gtctttagac acaggcttct cttctttctt cggaaataga
     1681 ttgaacttga agaatgttgt taaattcgct ataataataa agctatagac aagattattg
     1741 tttcagttga gactactgaa aataatacta agaaatggat aaaagctaag gattgaatat
     1801 gacattacaa gacgaaatca aaaaacgccg tacttttgcc atcatctctc acccggatgc
     1861 tggtaagacg actattactg agcaattatt atattttggt ggtgaaatta gagaagcagg
     1921 gacagtaaaa gggaaaaaat caggtacttt tgcaaagtcc gactggatgg atattgaaaa
     1981 gcaacggggt atctctgtta cttcatctgt tatgcaattt gattacgcgg gtaaacgtgt
     2041 taatattttg gatacacctg gacatgagga tttctcggaa gatacatacc ggaccttaat
     2101 ggctgtggat gcagctgtca tggttgtaga ttcggctaaa ggtattgagg ctcaaactaa
     2161 aaaacttttt gaagttgtta agcatcgtaa tattccggtt tttacgttta ttaataaatt
     2221 ggaccgtgat ggacgtgagc cactggattt gcttgaagag ttagaagaag tactaggtat
     2281 tgcttcttat ccaatgaatt ggccaattgg tatggggaaa tcatttgaag ggctttatga
     2341 tttacataat aaacgtttag aactctataa aggagacgag cgttttgcaa gtattgaaga
     2401 cggtgatcag ctgtttgcca ataatccatt ttatgaacaa gtcaaagaag atattgaact
     2461 tcttcaagaa gcaggtaatg acttttcaga acaagctatt cttgatggtg acctaactcc
     2521 agtattcttt ggctctgccc ttacgaactt tggagttcag acttttcttg atactttctt
     2581 ggagtttgct ccagaacctc atggtcacaa gactacagaa ggtaatgtta ttgacccctt
     2641 agctaaagat ttttcaggtt ttgtctttaa aattcaagct aacatggacc cacgtcatcg
     2701 cgaccgtatt gcttttgttc gtattgtttc tggtgaattt gagtgcggca tgggagttaa
     2761 tttgacacgc actggtaaag gcgctaagct ctccaatgtt actcaattta tggcagaatc
     2821 acgagaaaat gtgacgaatg ctgttgctgg ggatattatc ggggtttatg atacaggaac
     2881 ttatcaagtt ggggatacct taacggttgg gaaaaataaa tttgaatttg aaccattacc
     2941 aacttttacc ccagaattat ttatgaaagt atcagcaaaa aatgttatga agcagaaatc
     3001 attccacaag ggtattgagc aattagtaca agaaggagca atccagcttt ataagaatta
     3061 tcagacaggc gaatacatgc tgggtgctgt gggtcaactt cagtttgaag tttttaaaca
     3121 tcgtatggaa ggtgaataca atgctgaagt tgttatgaca ccaatgggta aaaagactgt
     3181 acgttggatt aactcagacg atttagacga acgcatgtcc tcaagccgaa atattttagc
     3241 aaaagatcgt tttgaccaac cagttttcct atttgaaaat gattttgcac ttcgctggtt
     3301 tgctgataag tatcctgatg tgaagcttga agaaaaaatg taaaatttaa agtcactcaa
     3361 atttgagtga ctttttaagt tttttagact aaaatagtta taatacatag aaaagattgg
     3421 agattaacat gggaattttt tcgggattaa tgggaaatgc ttcacaagtg gataccgata
     3481 aagtcgagaa tcagttatct gatattctga ttccaggtga acaggttgat ttagcttata
     3541 ctttaattcg tgatttgatt gtttttacta attatcgttt aattttagtc gataaacagg
     3601 gggtgacagg taaaaaggtt tcttataatt ctatacctta tgcttctata tcacgtttta
     3661 cagtggaaac tagtggtcat tttgatttag atgctgagtt aaagatttgg atatcatcag
     3721 caatagaacc agcagaagta cttcagttta agaacgaccg taacatagtg agtatacaga
     3781 aagctttggc gatagctgtt ttaaaataaa caatttagca agtgctgcta ctaagagtta
     3841 tttgttttaa ttataacttt taatagtgct aaattctcat tttcaaaata aaagtagtga
     3901 tttttatcac tgcttttatt ttgaagtata gtaggcctca agcatatatt ttgaaaacgc
     3961 tgtatttttt tgataagata tttttttgag gagtattaaa taatgataat agaattaaag
     4021 agtgttggga aaatttttcc aaaatcacag tttagattag atgatatttc ttttaatata
     4081 gcagaaggcg agatagttgg tctgattggt tctaatggta ctggaaagag tactattttg
     4141 aagatgatta atggcttaat cccttatgat aaaggaaata tttattatca aggaaaagaa
     4201 gtgaagtctt tttctgataa taagttacgt cagatgcgta aagatattgc ttatattttt
     4261 caaaatcata atttgttagc aggagaaagt gtatattatc atctagcttt agtttataaa
     4321 ttaaatcatc aaaaagtaaa tcatgacgca attaatgata tcttagattt tttaggtttg
     4381 atggatttaa aacaggtgaa atgccatagt ttgagtggtg ggcagcaaca aaaagttgca
     4441 atcgctatgg ctgttttgca aaaaccgaaa cttattctat gtgatgatat cagctctgcc
     4501 ttagatacta atagtgaaaa agagatattt aacttgttgt ctgatcttcg tgaaaaatat
     4561 ggtatctcta tcttgatgat tgctcatcac ctatctcttt taaaacaata ttgtgatcgt
     4621 gtaatgattt tggatcacca gactattgta gatactgttg ttccagtgaa agcaactctg
     4681 aatcaattaa agtcaaacta cgtagatcaa gtaaaggagt ttttgttaca tgattgacat
     4741 tattgaaaaa catggcgaag gtatcttaaa atcattttgg gaaaccaatt taatgttagg
     4801 gttaaccttg attctttgtt ttctaattgc ttttccaaca ggtattttat tgttttcatt
     4861 gagaaagtct tacttgatta aacattcttt agcctatcaa ctactaaatt tatttttagg
     4921 tacgttacgg agtgtccctt tccttatttt tatttttatc ttaatccctc tcaatcgatt
     4981 aatttttggg acctcatttg gaacgatagc ggcaatctta cctcttactt tggtttcagt
     5041 cagtttgtat gcacgttatg tggaacaagc tttattaaat atacctcaag ttgttgtaga
     5101 tcgagctctc agtctagggg ctaacaagag acagataatt tattattttt taatcccctc
     5161 catcaagatt gaccttgttt tatcatttac tgcaacggct ataagtattc ttggctactc
     5221 gacaataatg ggagttattg gtgcaggagg tttgggtgaa tatgcctatc gttttggcta
     5281 tcaagagtat gactacccag ttatgtactt gattgttgtc ttatttataa tatatgtgtt
     5341 tatcttacag agtttgggct attttatagc aaaccgctac tcaagaaaat aaaagaaaag
     5401 aggaactatg aaaaaattac tttccctaac atgtctaatc atgatgtctt tatgtttagt
     5461 ggcatgtact aagcaagcaa tgtcgtctaa gcaaattaaa gataagaata gtaaagaaaa
     5521 ggtgattact gttgcaactt acagcaaacc tacatctacc tttttagatt tgattaaaga
     5581 taatgtaaaa gaaaaaggat atactttaaa ggttgtcatg gtctctgact atattcaggc
     5641 taacattgct ttagaaaaca aagaacatga tgctaacctt ttacaacatg aatttttcat
     5701 gagtatcttt aataaggaaa atgatggtca tctagtgtca attacaccaa tttatcattc
     5761 attggctggt ttttatggtc aacatttgaa aaatattgcc gagcttaaag acggtgctaa
     5821 ggtagcgatt ccgtctgatc ctgccaatat gactagagct ctgctattat tgcaagaaaa
     5881 gaaacttatc accttaaaga atacgtccaa aaagaccaag gctatcgaag atattattac
     5941 taaccctaaa aaattacgaa ttgaacctgt agcattactt aacctcaatc aggcctattt
     6001 tgaatatgac cttgtcttta atttccctgg atatgtgaca aaaatcaatc tagttcctaa
     6061 aagggataga ttattatatg agaaaaaacc agatatccgt tttgcaggtg ccttggtagc
     6121 tcgtgaagat aataaaaata gtgataaaat aaaagtactt aaagaagtac taacaagtaa
     6181 agagattcgt cactatatca ctaaggagat tccaagtgaa gcagacgttg cgttctagat
     6241 agataaacta tgaaccctcc ttctttatag atggagggtt tttattgcct tttttctatt
     6301 ttctgaagca actaatagaa ctagcaattt atgaaagcgt gttatttatt agattgacct
     6361 tattttatat ctttttactt ttaattgtgt tacactaagt aggttgtcag aaaagataac
     6421 taataaaggt tcataaggca gcttcgcact gtcttttaga aaagagaaac atttgaaatt
     6481 tacagaactt aacttatcgc aagatattct ttcagcggta gaaaaagcag gctttgtaga
     6541 gccatcacca atccaagaaa tgactatccc acttgctctt gaaggcaaag atgtcattgg
     6601 tcaagcgcag acaggtacag gaaaaactgc agcttttggg ttaccaactt taaataaaat
     6661 tcatactgaa gataatacta ttcaagcact tattattgct cctacacgtg aacttgcagt
     6721 acaatcacaa gaagaattgt tccgttttgg acgcgataaa ggtgttaagg tccgctctgt
     6781 ttatggtggg tcaagtattg aaaaacaaat caaggcatta cgttcaggag cacacgttgt
     6841 tgttggaact ccaggtcgat tacttgattt aattaaacgt aaagcactta aacttaatca
     6901 catcgaaact cttattcttg atgaagcaga cgaaatgctt aacatgggat ttttagagga
     6961 cattgaagca atcattagtc gtgtaccaga agcacgtcaa actcttttat tctcagcgac
     7021 aatgcctgat ccaatcaaac gtatcggggt taaattcatg aaagatcctg agcatgttaa
     7081 aattaaagca actgaattaa ctaatgtgaa tgttgatcag tattatgttc gcgttaagga
     7141 aaatgaaaaa ttcgatacta tgacacgttt gatggacgtt gatcaaccag aattgtcaat
     7201 tgtctttgga cgtacgaaac gtcgtgtaga tgaattaact cgtggtctaa aattacgtgg
     7261 tttccgagca gaaggaattc atggagactt agatcaaaat aaacgtcttc gtgttatcag
     7321 agactttaaa aatgaccata ttgatattct tgttgccaca gatgttgctg cgcgtggttt
     7381 agatatttca ggcgttacac atgtttacaa ctatgatatt ccccaagatc cagaaagtta
     7441 tgttcaccgt attggacgta ctggtcgtgc tggtaaatct ggtcaatcta tcacttttgt
     7501 ttcaccaaac gaaatgggat atttgacaat tattgaaaac ttaactaaaa aacgtatgac
     7561 tggaatgaag cctgctactg caagcgaagc atttcaggct aagaaaaaag ttgcattgaa
     7621 acgtattgca cgtgactttg aggatcaaga attagtttct aaatttgata aatttaaagc
     7681 cgatgcttta gaattagcaa ctcaatatac accagaagaa cttgctcttt atgtcttaag
     7741 cttaacagtt caggatccag aatcgttacc agaagttgaa atcacacgtg aaaaaccatt
     7801 accatttaaa ccatcaggtg gtggctttaa aggtaaaggt ggacgaggta atggccgtgg
     7861 tggcgatcgt cgacgcaatg atcgtggtga tcgtcgtgga aatcgtgacc gtgatgatcg
     7921 tggaggtcgt cgtgatttta aacgtcgtga tgataagttc aagaaagata atcgtcgtca
     7981 agaaaataaa aaacctcata aaaacacttc aagtgaaaaa caaactggat ttgttattcg
     8041 caacaaaggc gataaataag aggattacga aaaaggagtt gctgagcgac tcctttttta
     8101 ttttttaaga taaagcgtag atagtgatat ccgtaaaatt gccttataac tttaagaatg
     8161 aagacaaaag attaaagtga ggttgaaatt taaaagttaa agaagcgagg acaggctcat
     8221 tgcacttagt atattatcgt attattgttc atttttatgt tgtttaatat aagtcaattt
     8281 tgctttccga gtcttttgtt taaataatgc ttctgccctc attgcttctt gtttgctatt
     8341 aaaagcttca gaataaagaa gttttactgg caatctcgca cgagtatatt tagcaccttt
     8401 tccggtattg tgtgtattga gacggcgctt gacatctgta gtataacctg tataaagtgt
     8461 tccatctgaa cattctaaaa catacatata agcaggaact ttaatcatga gattttccgt
     8521 tttccccgaa atatatatca aaaatagtct cagtatagtc tccattatct tgatgaacaa
     8581 caagaggagg aagtattttc ataccttctg tcgagccatc tttaatagct tcaattaaaa
     8641 gcatattagc atcctttcct aattttggat aaacaaattg gatacgttta ggtgctaaat
     8701 tgaactgacg catcgtatca ataatttcaa ggaaacggtc tggacgatgt accatagcga
     8761 tacgtccttt tgtttttaga gcatgttgag caatttgaca aatctctcta aggtttgttg
     8821 ttatttcatg tcttgcaagg agataatgcg ggcttagatt ttttttagag gtttcgcttg
     8881 ctttgaaata aggtggattg caaagcatta aatcaacgtt tgaacgttga acatgatcaa
     8941 gaagattttt taaatcatca ttaatcatag taacctgttt ttccaattta ttgagcttaa
     9001 ttgaacgctt agccatatcc gcaaggcgct cttgtagttc gatctctata attgtcgcac
     9061 ttgttttggt gctggcaaac aaaccgacag caccattacc actgcataag tcaacgataa
     9121 gaccacgaga aggaagttta gggaaccgag acaataaaac actatctatg gagtaggaaa
     9181 aaacttcttt gttttggatg attttgacat cagttgaaaa aagctgatca atgcgttcat
     9241 tttcttttaa aattgtatct atcataggct tattatagca aaattacagc ttcctagcac
     9301 tagttttgaa agtgtggagg ttgattttaa gataattctt tctaaaaaaa aggaagcatg
     9361 ataaaataga agttacttga tgaaaggtag aggagaaaca tgttctatac ttacttacgt
     9421 actttagtta tgttcttgat ttgggtggct aatggtaatg cgcactatca taatgaagat
     9481 aaaatgttaa aagatgatga aaattatatt ttagtagctc cacacagaac attttgggat
     9541 cctgtgtaca tggcttttgc tgctagaccc aaacagtttg tttttatggc caaaaaagaa
     9601 ttattcacga atcgtttgtt tggttggtgg attaaaatgt gcggtgcttt ccctattgat
     9661 cgtgaaaagc cagggcaaga cgctattcga tatcctgtaa agatgctgaa aaatagtaac
     9721 cgttctttgg tgatgtttcc gagtggaagc cgccactcca aagatgtcaa aggaggcgta
     9781 gctgttattg caaaaatggc aaaagtcaga attatgccag cagcatatag aggtcctatg
     9841 gtttttaaaa atctattaaa ggggcatcgt gttgatatga attttggtaa ccctattgat
     9901 gtttctgata ttaagcgtat ggatgccgaa gggattgcag aagtatctcg tcgtattcag
     9961 gaagaatttg atcgtttaga tagagaaaat gaaacttatg atgatggtaa gaaattaaat
    10021 ccattgactt atatttatcg tctaccttta gcaattatag ctattgtctt gcttgtccta
    10081 actctaattt ttagttattt agcaagcttt gtgtgggatc cacaaaaaca tctaaaataa
    10141 gggttacatg gaaactattt actttttagt aaatagtttt ttttgttttt aataggaaaa
    10201 tctctttagt tacgaataag taaatggagg aatattttta tgtttgaaat agtattggaa
    10261 aaaattaaat cgcataaatg ggagacaaca ggaattattg taggactctt attgtttgga
    10321 attttgggtt taaatcattt tggcactcat cataaagaag acgatttaaa tatcaattta
    10381 gagaaaaaag tatcaactat aacagaaaaa aaggtaccaa tgattagtca cgtcaaagat
    10441 aaagtatcta atcaagtaac ggttgacgta aaaggggcag tcaatcatcc aggtgtttat
    10501 agtttgcctt ctcaaagtcg tgtaacagat gcgattaagc gtgcaggtgg actaagcaat
    10561 ctcgctgata gtaaatccgt taatctagca cagaaattac aagatgagac ggttatctat
    10621 gttgctcaaa aaggtgaaaa aataactgtt gtggaagagg agaaagcaaa taatattgct
    10681 actcagggaa atagtaaggg gaaaattaat cttaataaag cagatttatc aagtcttcag
    10741 accatatcag gcgttggagc taaaagagct caagatattt tagattatcg tgatagtcaa
    10801 ggtggtttta aaactattga tgacctcaaa aatgtatcag gtattggtga gaaaacacta
    10861 gaaaaattac gtcaagatgt tacacttgac taagtatttt cctctaaaac ctatttattt
    10921 agcattgttg gtcttccaaa tttacttact agtgttttct tggacaatgc ttggttgtgc
    10981 ctttctttta tttactttta tttttc
//
LOCUS       DBJYSH010000068        10871 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.068, whole genome shotgun sequence.
ACCESSION   DBJYSH010000068 DBJYSH010000000
VERSION     DBJYSH010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 10871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10871
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.068"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            126..1271
                     /locus_tag="ACUBV3_001368"
     CDS             126..1271
                     /locus_tag="ACUBV3_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HHU3847866.1"
                     /translation="MIYFDNSATTIPYPEVLRTYQEVASKIYGNPSSLHELGTTSSRI
                     LEASRKQIASLLELKANEIFFTSGGTEADNWVIKGLAFEKQHFGNHIIVSDIEHPAVK
                     ESAKWLGEYGFEIDYAPVDDKGFVDVEALVKLIKPETILISIMAINNEIGSIQPIKAI
                     SDLLSDKPTISFHVDAVQAIGKIPTKDYLTERVDFASFSSHKFHGVRGVGFLYIKEGK
                     RISPLLTGGGQESDLRSTTENVAGIAATAKALRMVMDKEVVAIPKISKMKTIIHDELA
                     KYEDITLFSGKEGFSPNIITFGIKGVRGEVLVHAFEGHDIFISTTSACSSKAGKPAGT
                     LIAMGISTKLAQTAVRISLDDDNDMGQVEQFLTIFKQVYEKTKKVRG"
     gene            1273..2487
                     /gene="thiI"
                     /locus_tag="ACUBV3_001369"
     CDS             1273..2487
                     /gene="thiI"
                     /locus_tag="ACUBV3_001369"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HHU3847867.1"
                     /translation="MQYSEIMIRYGELSTKKKNRMRFINKLKNNMEHVLSIYPDVSVK
                     TDRDRGHVYLNGTDYHEVAESLKEIFGIQAFSPSFKVEKNVDTLVKAVQEIMTSVYKD
                     GMTFKITAKRSDHSFELDSRALNHTLGDAVFSVLPNIKAQMKQPDINLKVEIRDEAAY
                     ISYEDIRGAGGLPVGTSGKGMLMLSGGIDSPVAGYLALKRGVDIEAVHFASPPYTSPG
                     ALKKAHDLTRKLTKFGGNIQFIEVPFTEIQEEIKEKAPEAYLMTLTRRFMMRITDRIR
                     EDRNGLVIINGESLGQVASQTLESMQAINAVTATPIIRPVVTMDKLEIIDIAQKIDTF
                     DISIQPFEDCCTIFAPDRPKTNPKIKNTEQYEKRMDVEGLVERAVAGIMVTTIQPQAD
                     SDDVDDLIDDLL"
     gene            2589..3767
                     /locus_tag="ACUBV3_001370"
     CDS             2589..3767
                     /locus_tag="ACUBV3_001370"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000101234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HHU3847868.1"
                     /translation="MARIPYQKTLIFCVAVIIAIFILGLSKDLAQSKGQKVANNNTVK
                     TARVVANGDILLHDVLYASARQPDGTYNFTPYFKEVKSWIESADLAIGDYEGTISSEY
                     PLAGYPLFNAPNEIATTMKETGYDVVDLAHNHILDSQLAGAINTVKTFNRLGLDTIGV
                     YLKDRNKEDILIKRVNGIKIAILGYSYGYNGMEANVSKSDYEKHMSDLDTKKIKQDIK
                     KAEKEADITIVMPQMGIEYQKKPTTEQVILYHSMIKWGADIIFGGHPHVVEPSEVIKK
                     DGQKKFIIYSMGNFISNQRLETVDDIWTERGLLMDVTIEKKGQKTVIKKVKAHPTLVE
                     AKANGRYVEEGFPAYDYRTLVLEDYIEGGKKRSTIDQTMKDKVDKAYKEMNELVNLKW
                     "
     misc_feature    3843..3922
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            3957..4271
                     /gene="rplU"
                     /locus_tag="ACUBV3_001371"
     CDS             3957..4271
                     /gene="rplU"
                     /locus_tag="ACUBV3_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HHU3847869.1"
                     /translation="MSTYAIIKTGGKQVKVEVGQAIYVEKLDVEAGAEVTFNEVVLVG
                     GETTKVGTPVVEGATVVGTVEKQGKQKKVVSYKYKPKKGSHRKQGHRQPYTKVVINAI
                     NA"
     gene            4278..4616
                     /locus_tag="ACUBV3_001372"
     CDS             4278..4616
                     /locus_tag="ACUBV3_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HHU3847870.1"
                     /translation="MIKATFTRNQSGYLYSAEISGHAGSGEYGFDVICAAVSTLSINF
                     INSLEALTTCQAQLIINDVEGGYMKIDLSSIPQHKEDKVQLLFESYLLGMTNLSKDSS
                     EFVSTVVMTH"
     gene            4638..4931
                     /gene="rpmA"
                     /locus_tag="ACUBV3_001373"
     CDS             4638..4931
                     /gene="rpmA"
                     /locus_tag="ACUBV3_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HHU3847871.1"
                     /translation="MLKMNLANLQLFAHKKGGGSTSNGRDSQAKRLGAKAADGQTVSG
                     GSILYRQRGTHIYPGANVGRGGDDTLFAKVEGVVRFERKGRDKKQVSVYPIAK"
     gene            5147..6052
                     /locus_tag="ACUBV3_001374"
     CDS             5147..6052
                     /locus_tag="ACUBV3_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HHU3847872.1"
                     /translation="MNIQQLRYVVAIANSGTFREAAAKLFVSQPSLSVAVRDLETELG
                     FQIFTRTTTGAVLTNQGMTFYENALEVVKSFDSFEKQFSQSEATEQEFSIASQHYDFL
                     PPLITAFSKCNDNFSYFRIFESTTIRILDEVAQGNSEIGIIYINSQNKKGLLQRLDKL
                     GLEFVELIPFKTHIYLGKDHPLASKTSLIMTDLEGLPTVRFTQDRDDYRYYSENFVEV
                     LDSSVTYNVTDRATLNGILERTQAYATGSGFLDSRSVNGITVIPLEDHLDNQMIYIKR
                     KDRNLSQMALKFVAVMEEYFKGFNS"
     gene            6061..6525
                     /gene="lspA"
                     /locus_tag="ACUBV3_001375"
     CDS             6061..6525
                     /gene="lspA"
                     /locus_tag="ACUBV3_001375"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HHU3847873.1"
                     /translation="MRKIIIPIITILLIALDQLSKLWIVKHIELNQIKEFIPNIVSLT
                     YLRNYGAAFSILQNQQWLFTLITIFVVCVAIIYLMKHINGSYWLLISLTLIISGGLGN
                     FIDRLRLGYVVDMVHLDFINFAIFNVADSYLTIGIICLMIALWKEESNGNHN"
     gene            6509..7399
                     /locus_tag="ACUBV3_001376"
     CDS             6509..7399
                     /locus_tag="ACUBV3_001376"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HHU3847874.1"
                     /translation="MEITIKIAGVRLDKALADNSELSRSQANEEIKKGIVLVNGQVKK
                     AKYTVQEGDRITFDIPKEEVLDYQAENIPLDIIYQDDDVAVVNKPQGMVVHPSAGHSS
                     GTLVNALMYHIKDLSSINGVVRPGIVHRIDKDTSGLLMVAKNDRAHQVLAEELKDKKS
                     LRKYLAIVHGNLPNDRGVIEAPIGRSDKDRKKQAVTAKGKPAITRFHVLERFGDYTLV
                     ELSLETGRTHQIRVHMAYIGHPLAGDPVYGPRKTLGGKGQFLHAQTLGFTHPSNGENL
                     IFSVEVPEIFQTTLDKLRKN"
     gene            7575..8096
                     /gene="pyrR"
                     /locus_tag="ACUBV3_001377"
     CDS             7575..8096
                     /gene="pyrR"
                     /locus_tag="ACUBV3_001377"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HHU3847875.1"
                     /translation="MKRKEIIDDVTMKRAITRITYEIIERNKNLDNIVLAGIKTRGVF
                     LAKRIQERLKQLENLDIPVGELDTKPFRDDMKVEVDTTTMPVDITDKDIILIDDVLYT
                     GRTIRAAIDNLVSLGRPSRVSLAVLIDRGHRELPIRADYVGKNIPTSQFEEILVEVME
                     HDGYDRVSIIDPS"
     gene            8145..9215
                     /locus_tag="ACUBV3_001378"
     CDS             8145..9215
                     /locus_tag="ACUBV3_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HHU3847876.1"
                     /translation="MAKKLLILEDGTVFEGLSFGSSLDVTGELVFCTGNTGYQEIITN
                     PSHNGKILVFTSPLIGNYGIHRSYSEAIIPTCLGVVVAEYSRCVSSDTSKMNLDEFLK
                     MKKVPAMSGVDTRYLMQVIKEKGFVKATLAEAGDVLSHLQDQLIATVLPTNNVEQVST
                     KTAYPSPASGRNIVVLDFGLKHSVLRELSKRQCDVTVIPYNTSLEGIKNLYPEGIILS
                     NGPGNPEKLQEVLNTIKELQKSVPMLGIGLGHQLIAMANGAEIMRLPVAKKGPNYPMR
                     DIATGRLETVIQFNHFTVNRLNLPHDLLVTHEGLNDQEIVALRHRSFPVMSVQFYPEA
                     APGPHDVTYFFDEFLEMIGSRA"
     gene            9271..10869
                     /locus_tag="ACUBV3_001379"
     CDS             9271..10869
                     /locus_tag="ACUBV3_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase large subunit"
                     /protein_id="HHU3847877.1"
                     /translation="MLNTKDKQPILLIIFGQKMKEYDDLIRSTVERLSKRNRPVSILI
                     SKELNHVIHHYDAQFENLETLVGYINTHEDSLIYPLCLDKNHELFQEFLAWQSHYSRQ
                     YISDNLSEKSVQAIDNNQDFVQVDCLVMRDSLNNCLYVSDLEYIESNKTTGKSLAIVP
                     SQTLSDAARQTIRDVAFDVCRKANIIGVCYFSFLIDLNSLDYHIISLSSGLSYQSILF
                     ETITTYPVLEIATKLTVGYTFSQLKHSYYPNTSAFLEPQLDYVAAVSFSFEKVDYIFF
                     ARNIEQLFLNLLEASSHDHFPFLSDISEEDLMFALIQKKENRLAYLLEAFRRGFDLYD
                     LSSVTKINPFYLDKCLHIVELYENLNKSQYNVDIYKDAKRYGFSDDYIASSWQISLID
                     MLEYRKKHSVAPVLKQVEQSSGVLTGHQIQYFRSYDWHSDYSSSGCQKALIMVDKGYS
                     LVKLNELIKQIKQTHLELLIVTNQPLLIEQLNDTSIIFDTIGIETILTIMGIEEVEEV
                     YLLGESQADMFSKMKNFSISCQSI"
BASE COUNT         3594 a         1613 c         2188 g         3476 t
ORIGIN      
        1 gtagtgagtt ttgtgataaa ttatgagaat tatcataaat attgttttgg gatgttcaca
       61 aactaatata aatagtagga aaattatgct ataataacac tgattgactt tagaaggaca
      121 gatttatgat ttattttgat aattcggcaa ctactattcc ctatccagaa gttttaagga
      181 cttatcaaga agtagctagt aaaatctatg gaaacccctc aagtttacat gagctaggaa
      241 caacatcttc tcggatttta gaggctagcc gtaaacaaat tgctagttta cttgagctta
      301 aagcaaatga aattttcttt acctctggtg gtacagaggc cgataattgg gttattaagg
      361 gtcttgcgtt tgagaaacaa cattttggaa atcatattat tgtttctgat atcgaacacc
      421 cagctgttaa agagtctgct aaatggcttg gagagtatgg gtttgagatt gattatgcgc
      481 cagtagatga taaaggattt gtagacgtag aagcactggt caaattaatt aaaccagaaa
      541 cgattcttat ttcaattatg gcaataaaca atgaaattgg ttctattcag ccaataaaag
      601 ctatttcaga cttgttgtca gataaaccta ctatatcgtt tcatgttgat gctgttcaag
      661 caataggtaa gataccgact aaagattatt tgactgagcg agttgatttt gcatcttttt
      721 ctagtcataa atttcacggc gttcgtgggg ttggctttct atatattaaa gaaggaaaac
      781 gtatttcacc attactaaca ggtggtggac aagaatctga tttacgttca actactgaga
      841 atgtcgcagg gattgcagct actgctaaag ctttacgaat ggttatggac aaggaagtag
      901 tagctattcc taaaataagt aaaatgaaaa ccattattca tgatgaatta gccaaatatg
      961 aggatattac gttattttcg ggtaaggaag gtttttcacc taacattatc acgtttggta
     1021 tcaagggtgt ccgaggagaa gttcttgtcc atgcctttga gggacatgat atttttatct
     1081 cgacaactag cgcttgctct tctaaagcag gtaaaccagc aggaactctt atagcaatgg
     1141 gaatttcaac taagttagcc caaactgctg tacgtatcag cttagatgat gataatgata
     1201 tgggacaagt agagcaattt ttaactattt ttaaacaagt ttatgaaaaa actaagaaag
     1261 ttagaggata agatgcagta ttcagaaatt atgattcgtt atggagaact ctctactaag
     1321 aagaaaaacc gtatgcgctt catcaataag ttaaaaaata atatggagca tgtactctcc
     1381 atttatccag atgtttcagt aaaaacagat cgtgatagag gacatgtata tctcaatggt
     1441 acagattatc atgaagttgc agagtcctta aaagagattt ttggtatcca agctttttct
     1501 ccatctttta aagtagaaaa aaatgttgat acattggtaa aagctgtcca ggaaattatg
     1561 acttccgttt ataaagatgg gatgactttt aaaattaccg caaaacgtag tgaccactca
     1621 tttgaattgg atagccgtgc tctaaatcat actttaggag atgccgtttt ttcagtcttg
     1681 ccaaatatta aggctcagat gaagcaacca gatatcaatc ttaaagtcga gatacgagat
     1741 gaggctgctt atatttcata tgaggatatt aggggtgcag gaggattacc ggtaggaaca
     1801 tctgggaaag ggatgctgat gttgtctggt gggattgatt ctccggtagc aggttaccta
     1861 gcgttaaaac gtggtgtaga tatagaagca gtccattttg caagtcctcc ttatactagc
     1921 ccaggtgcat tgaaaaaagc acatgattta acacgtaaat tgacaaaatt tggtggtaat
     1981 attcaattta ttgaagttcc attcacagaa attcaagagg aaattaagga aaaagctcct
     2041 gaagcctact tgatgacgtt aacacgtagg tttatgatgc gtattacaga tcgtattcgt
     2101 gaggaccgaa atggtcttgt tattattaac ggtgaaagtt tagggcaggt ggcaagccaa
     2161 acgctagaaa gtatgcaagc tattaatgct gtcactgcaa caccgattat tcgtcctgtg
     2221 gtcacgatgg ataagctaga aattattgat attgctcaaa aaatagatac ttttgatatt
     2281 tcaattcaac catttgagga ttgctgtaca atttttgcac cagatcgccc aaaaactaac
     2341 cctaaaatta agaatacaga acagtatgag aaacgtatgg atgtagaagg tctggtagag
     2401 agggcagttg cagggattat ggtaactact attcaacctc aagcagatag tgatgatgtt
     2461 gatgacttga ttgacgattt attataatga atttttaaac tacagattta ctctgtagtt
     2521 ttttattaaa atgattgtgc tgtcataagg ttataaaaaa tgctataata cgaataaagg
     2581 aggcaagtat ggcaagaatc ccttatcaaa aaacacttat tttttgtgta gccgttatta
     2641 ttgctatttt tattttgggg ttatctaagg atttagcaca atctaaaggg caaaaggttg
     2701 caaataataa tactgtaaaa acagcacgtg tggttgcaaa cggagatatt ttgctgcacg
     2761 atgtactata tgccagtgcg cgccaacccg acggcactta taattttact ccttatttta
     2821 aagaggtaaa atcttggatt gagagcgctg atttagcgat tggtgattat gaagggacga
     2881 ttagttcaga atatccttta gcgggctatc ctttatttaa tgctcctaat gagattgcaa
     2941 ctactatgaa agaaactgga tatgatgtcg ttgacttagc acataatcat attctggatt
     3001 cacaactggc aggtgctatc aatactgtca agacttttaa tcgtctggga ttggatacta
     3061 ttggggttta tttaaaggat agaaataaag aagatatctt aattaagcgt gtaaatggta
     3121 ttaaaattgc tattttaggt tattcgtatg gctacaatgg tatggaagct aatgtttcaa
     3181 agagtgacta tgaaaaacat atgtctgatt tagatactaa aaagattaag caagatatta
     3241 aaaaggctga aaaagaagca gatatcacca ttgtcatgcc acagatgggg atagaatatc
     3301 aaaaaaaacc tactacagaa caagtaatac tttatcatag tatgattaaa tggggggcag
     3361 atattatatt cggaggacac cctcacgttg ttgaaccgtc tgaagtgata aaaaaagatg
     3421 gccagaaaaa atttattatt tactctatgg gaaattttat ttcaaatcag cgtttagaaa
     3481 cggttgatga tatctggaca gaaagaggat tattaatgga tgtaaccata gagaaaaaag
     3541 gtcaaaaaac agtaattaaa aaagttaagg cgcatccaac actagtagaa gcaaaagcaa
     3601 atggccgtta tgttgaagaa ggctttccag cttatgatta taggacgcta gttttagagg
     3661 attatattga aggtggtaag aaacgttcta caattgatca gactatgaaa gataaagttg
     3721 ataaagcata taaggaaatg aatgagttag ttaatttgaa gtggtaaagt tgataatttt
     3781 attttgtttt atcttgcata aaaaaagagt ttatgataaa atttgaatgt tgactaaatg
     3841 cacatcctgt gcaaccgcgc gaaaatcgtt agattaagta gggcaaggct ctcacgattt
     3901 ataggcgagt cttcacagag ggaaacctca caagaaattt ttataggagg tgcatcatga
     3961 gcacatacgc aatcatcaaa actggtggta aacaagttaa agttgaagta ggacaagcta
     4021 tctacgttga aaaacttgac gttgaagcag gagcagaagt tacatttaac gaagttgttc
     4081 ttgttggtgg tgaaacaact aaagttggta ctccagttgt tgaaggagct actgttgttg
     4141 gtactgttga aaaacaaggc aaacaaaaga aagttgtttc ttacaaatac aaacctaaaa
     4201 aaggtagcca ccgtaaacaa ggtcaccgtc aaccttatac aaaagttgtt atcaacgcaa
     4261 tcaacgctta atttttcatg attaaagcaa cttttactcg taatcaatca ggctatcttt
     4321 atagtgctga aatcagcggt catgctggta gtggcgagta cggctttgat gtgatttgtg
     4381 cagctgttag tactttatca attaatttta ttaattcatt agaggcttta acaacctgcc
     4441 aagcgcaatt gattatcaat gacgttgaag gtggttatat gaagatagat ttatcttcta
     4501 taccacaaca taaagaggat aaggttcaat tattatttga gtcttacctt cttggaatga
     4561 ctaatctgtc taaagattct tctgaatttg ttagtacagt agtcatgaca cactaattta
     4621 agagaggaca caacattatg ttaaaaatga atcttgctaa cttgcaactt ttcgcccaca
     4681 aaaaaggtgg aggttctaca tcaaacggac gtgattcaca agcaaaacgt cttggagcta
     4741 aagcagctga cggacaaaca gtatcaggtg gatcaattct ttaccgtcaa cgcggtacac
     4801 acatctatcc aggtgctaac gttggacgtg gtggggacga tacacttttt gctaaagttg
     4861 aaggtgttgt tcgtttcgaa cgtaaaggac gcgataaaaa acaagtgtct gtttacccaa
     4921 tcgcaaaata atgcctagtt aagactttat gactattcat aaggtctttt attattgtca
     4981 gagctttcat tttgtaacaa aagacaatat gttaagagat ttatcattaa tactcataaa
     5041 ctttgttgtt cttttatagt gaaatagtcc taattcatga agaattttcc gcaaggatag
     5101 ttattttttg gtataatgaa gggtactaaa atgaggaagt gataaaatga atattcaaca
     5161 actgcgttat gttgttgcta tcgcaaatag tggtactttt cgtgaagcag ctgcaaaact
     5221 atttgttagt cagccaagtt tatctgttgc cgttcgtgat ttggaaacag aattagggtt
     5281 tcagattttt acgcgtacaa ctactggtgc agtgttgacc aatcaaggca tgacttttta
     5341 tgagaatgct ttagaagttg tgaaaagctt tgattcattc gaaaaacagt tttcgcaatc
     5401 tgaagctaca gagcaagaat tctcaatagc aagtcaacat tatgattttc tacctccttt
     5461 aatcacagcg ttttcaaaat gtaatgataa ttttagctat ttccgtattt tcgaatcaac
     5521 aacaatacgt atcttagatg aagttgctca agggaatagc gaaataggta tcatttatat
     5581 caatagtcaa aataaaaaag gtctacttca gcggttggac aaattggggt tagagtttgt
     5641 agagcttatt ccttttaaaa ctcatattta tcttgggaaa gaccatccct tggctagtaa
     5701 aacatctctg ataatgacag atttagaagg attaccaacc gtacgtttta cacaggatag
     5761 agatgattat cggtattact cggagaattt tgtggaagta cttgatagtt ctgttaccta
     5821 taatgttact gatagagcaa cattaaatgg tattttagaa agaacccaag cttatgcgac
     5881 gggctcagga ttcttggata gccgcagtgt gaatggtatc actgttattc cattagaaga
     5941 tcatctagat aaccaaatga tttatatcaa acgaaaagat cgtaatttat ctcagatggc
     6001 tttaaaattt gttgctgtta tggaagagta ttttaaggga tttaattcat aggagaagta
     6061 atgagaaaga ttattatacc aataataact attttgttga ttgctttaga tcaactatct
     6121 aagttatgga ttgttaaaca tattgaacta aaccaaatta aggaattcat tccaaatatc
     6181 gttagtctaa cttatctgag aaattatggt gctgcatttt ctattttgca aaatcaacaa
     6241 tggcttttta ccttaattac aatctttgtt gtttgtgttg ctattattta tttgatgaaa
     6301 catattaatg gaagttattg gttattaatt tctctaacct taatcatctc tggtggatta
     6361 ggaaacttta tagatcgctt aagattaggt tatgtcgttg atatggtgca tttagatttt
     6421 attaattttg ctatctttaa tgtagcagac tcatatctga caatcggtat tatttgctta
     6481 atgattgcac tttggaaaga ggaaagtaat ggaaatcaca attaaaatag caggagttag
     6541 gctagataaa gctttagctg ataattctga actgtcgcgt agtcaagcta atgaagaaat
     6601 taaaaaaggg attgtgttag taaatggaca agtaaaaaaa gcgaaatata ctgttcaaga
     6661 aggagatcgg attacttttg atattcctaa agaggaagta ctagattatc aagctgaaaa
     6721 tatcccttta gatattatct atcaagatga tgacgttgct gtggttaata agccgcaagg
     6781 gatggttgtt cacccttctg caggccacag ttcgggcacc ttagttaacg ccttgatgta
     6841 tcatattaag gatttatcat caattaatgg cgttgttcgc ccagggatcg ttcatcgtat
     6901 tgataaagat acttctggtt tattgatggt tgcaaaaaat gatagagctc accaagtgtt
     6961 ggctgaagag ttgaaagata aaaaatcgct tcgtaaatat ttagcaattg tgcatggtaa
     7021 tttaccaaat gatcgaggcg tgattgaggc tccgattggt agaagtgata aagatcgtaa
     7081 gaagcaagcc gtgactgcta aaggtaagcc agcaataact cgttttcatg ttttagaacg
     7141 ttttggtgat tataccttag ttgaattgtc attggagaca ggtcgaacgc accaaattcg
     7201 ggttcatatg gcttatattg gccatcctct agccggtgat cctgtctacg gtccccgcaa
     7261 gactttaggg ggtaaaggac agtttttgca tgcacaaaca ctaggtttta cgcaccctag
     7321 taacggagag aatttgattt tttcagtaga ggttccggaa atttttcaga caactttaga
     7381 caagttgaga aaaaattaag tttttcttga aaaatacttt taccatttgc tataatataa
     7441 gcaaatcatt cctttaacct tatccagtga gatagttaag gagaaacttc agactaaatc
     7501 aatgcctgag cggctttgtc attctagaga gtctcctttt gaaggggctc ttttatctta
     7561 gagaggatac tgcaatgaaa agaaaagaaa ttattgatga tgtgaccatg aaacgtgcta
     7621 ttacacgtat tacctacgaa attatagaac gtaacaaaaa tttagataat attgttttag
     7681 caggcattaa aacaagaggt gtttttttag ccaaacgcat tcaagaacgt ttgaagcaat
     7741 tagaaaattt agatattccc gttggagaat tggatactaa acctttccgt gatgatatga
     7801 aagttgaagt tgacaccaca actatgccag ttgacatcac tgataaagat attatattaa
     7861 ttgatgatgt tctctataca ggtcgcacta ttcgagctgc tattgataat cttgttagtt
     7921 tggggcgtcc gagtcgtgtc agtttagctg tattaattga ccgtgggcat cgtgaactac
     7981 ccattcgtgc agattatgtg gggaaaaata ttccaacaag tcaatttgaa gaaattctag
     8041 ttgaagtgat ggaacatgat ggttacgatc gtgtgagtat tattgatccg agttaagtag
     8101 acgactatta tttacgatga ttttatagaa ggggaagaac cacaatggca aaaaaattgt
     8161 taattctaga ggatggtacg gtttttgaag gactatcctt tggttcatct cttgatgtaa
     8221 ctggcgaatt agttttttgt acggggaata ccggctatca ggaaataata acgaatcctt
     8281 cacataatgg aaaaatttta gtttttacct caccgctaat aggaaattat ggtattcatc
     8341 gtagttactc tgaagccatt attcccactt gtttaggagt tgttgttgca gagtattcca
     8401 gatgtgtaag tagcgatact agcaagatga atctagatga atttttgaaa atgaaaaagg
     8461 ttcctgcaat gtcgggggtc gatacaaggt atttgatgca agttattaaa gaaaaaggat
     8521 ttgtaaaagc tactttagca gaagcaggtg atgttttatc acatttacaa gatcaattaa
     8581 tagcgacagt attaccgaca aataatgtgg aacaagtttc gacaaaaaca gcttatccgt
     8641 cgcctgctag cggtcgaaat attgttgtac ttgattttgg ccttaaacat tctgttttga
     8701 gagaattgtc taaacgtcag tgtgacgtga ctgtcattcc ctacaatacg tctttagaag
     8761 gtatcaaaaa tctataccca gaaggtatca tattatctaa tggtccggga aatccagaaa
     8821 agcttcagga agtcttaaat actattaaag aattacaaaa atctgttcct atgttaggta
     8881 ttggtttggg gcatcaatta attgccatgg caaatggtgc agaaattatg agactcccag
     8941 ttgctaagaa ggggccaaat tatcccatga gagatattgc tacggggcgt ttagaaacag
     9001 ttattcagtt taatcatttt accgttaacc ggctaaatct tccgcatgat ttactggtaa
     9061 cacacgaagg tttgaatgat caagagattg ttgctctaag gcaccgctca tttccagtga
     9121 tgtccgtgca attttaccca gaggctgctc caggccctca tgatgtgacc tatttttttg
     9181 atgaattttt agaaatgatt ggatcacgcg cttaaacata aggtattatt taaagtcagt
     9241 cggtgagctg attaataagg aggtgcgtat atgctaaata caaaggataa acagcctatt
     9301 ttgcttatta ttttcggaca gaaaatgaaa gaatatgacg atttaattag atcaactgtt
     9361 gagaggttat ctaaaaggaa tagacctgtt agtattctta tctcgaagga gctaaaccat
     9421 gtgattcacc attatgatgc acaatttgaa aatcttgaga cgcttgtggg gtatataaat
     9481 acacatgaag atagtttgat ttatcccctt tgcttagata aaaatcatga gctatttcaa
     9541 gagtttttag cgtggcagag tcattattca aggcagtata tcagtgataa tttatcagaa
     9601 aaaagtgttc aggcaataga caataatcaa gactttgtgc aagtagattg tttagtaatg
     9661 agagactctt tgaataattg tttatatgtc tctgatttag aatatattga atctaataaa
     9721 acaactggta agtctctagc gattgtacca agtcaaactt tatccgatgc tgctaggcaa
     9781 acaattcgag atgttgcatt tgatgtgtgt cgaaaggcaa acattattgg agtatgttac
     9841 tttagtttct taatagattt gaacagtttg gattatcata taattagctt atcatctggt
     9901 ttgtcttatc aaagcatttt atttgagact attacgacct atcctgtttt agagatagca
     9961 actaaattga cagtgggata tacttttagt caactgaagc attcttatta cccaaatacc
    10021 tctgcctttt tagagccaca attagactat gtagctgccg tatcttttag ttttgaaaaa
    10081 gttgactata tcttttttgc tcgtaatata gagcaattat ttttaaattt actcgaggct
    10141 agcagtcatg atcattttcc atttctgagt gatattagcg aggaagattt gatgtttgct
    10201 ttgattcaga aaaaagaaaa tcgtctagct tatcttttag aggcttttag acgtggcttt
    10261 gatttgtatg atttatcctc agttactaaa atcaaccctt tttacttaga taaatgttta
    10321 catattgtag agttgtatga aaacctgaat aaaagccaat ataatgtaga tatttacaag
    10381 gatgctaaaa ggtatggttt ttcggatgac tatattgcta gtagttggca aatatctttg
    10441 atagacatgt tagaatatcg aaaaaaacat agtgtcgcac ctgttctgaa gcaagtcgaa
    10501 caaagttctg gtgttctaac tggccatcaa attcagtatt ttcgaagcta tgattggcat
    10561 tcagactaca gttctagcgg ttgtcaaaag gctttgataa tggtagacaa gggttattcc
    10621 ctagtaaaat taaatgagtt aattaagcaa atcaaacaaa ctcatttaga attattaatt
    10681 gttaccaatc aacctctcct catagaacaa ttaaatgata catctataat ttttgacact
    10741 ataggtattg agaccatatt aacaattatg ggtatagaag aggtggaaga agtgtattta
    10801 ttgggagaaa gtcaagcaga catgttttca aaaatgaaaa atttttctat ctcttgtcag
    10861 tctatttagc a
//
LOCUS       DBJYSH010000069        10741 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.069, whole genome shotgun sequence.
ACCESSION   DBJYSH010000069 DBJYSH010000000
VERSION     DBJYSH010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 10741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10741
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.069"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            319..1488
                     /locus_tag="ACUBV3_001380"
     CDS             319..1488
                     /locus_tag="ACUBV3_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HHU3847878.1"
                     /translation="MAWLGNFFTGASFSLVMPFMVLYVEQLGAPSNKVEWYAGLSVSL
                     SALSSALVAPLWGRLADKYGRKPMMVRAGLMMTFTMGGLAFIHSVTGLLILRILNGIF
                     AGYVPNSTALIASQAPQEESGYALGTLATGVTGGMLIGPLLGGLLAEWFGIREVFLLV
                     GTILLISTLMTIFMVKEDFKPISNEETMPTTEVFKSVKSLQILIGLFVTSMIIQISAQ
                     SIAPILTLYIRHLGQTENLMFVSGLIVSGMGFSSILSSPKLGRIGDRIGNHRLLLLAL
                     LYSFLMYVLCSLAQTSLQLGVIRFLYGFGTGALMPSINSILTKIAPRQGLSRIFSYNQ
                     MFSNLGQVLGPFVGSAVSIHLGFRWVFFVTSFIVLANFVWCFINFRKYIRVKEIV"
     gene            1485..1631
                     /gene="rpmG"
                     /locus_tag="ACUBV3_001381"
     CDS             1485..1631
                     /gene="rpmG"
                     /locus_tag="ACUBV3_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HHU3847879.1"
                     /translation="MRVKINLKCSSCGSKNYLTSKNKSNHPDKIEVPKYCPKERKVTL
                     HIES"
     gene            1678..1914
                     /gene="secG"
                     /locus_tag="ACUBV3_001382"
     CDS             1678..1914
                     /gene="secG"
                     /locus_tag="ACUBV3_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HHU3847880.1"
                     /translation="MYNLLLTILLVLSVLLIISIFMQPQKNPSSNVFDSSGSEALFER
                     SKARGFEAFMQRFTGVLVFFWLLIGLVLSILSSH"
     gene            2027..4432
                     /gene="rnr"
                     /locus_tag="ACUBV3_001383"
     CDS             2027..4432
                     /gene="rnr"
                     /locus_tag="ACUBV3_001383"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HHU3847881.1"
                     /translation="MKEKIIKYLEEHGKTSVNDLAAALDMAGAKAFPKLIKTISNLES
                     HRQLRFDDNGSLSLQKKEAKKKEITVRGLFRANKAGFGFLSIDQDEDDMFIGKNDIAY
                     AIDGDTVEAVVKKPADRLNGTAAEARVVNIVERSLKTLVGKFVLDDERPKYAGYIKSK
                     NQKINQKIYIRKEPVVLDGTEIIKVDIDKYPTRGHDYFVASVRDIVGHQGDVGIDVLE
                     VLESMDIVSEFPEDVIAEANAIPDAPTEKDLIGRVDLRQEVTFTIDGADAKDLDDAVH
                     IKLLDNGHFELGVHIADVSYYVTEGSALNREALSRGTSVYVTDRVVPMLPERLSNGIC
                     SLNPNLDRLTQSCIMEIDQNGRVVNHQITQSVINTTYRMTYIAVNDIIAGDEEICSEY
                     ESIVSSVQHMVTLHHTLEAMRTRRGALNFDTSEAKIMVNDKGMPVDIVIRNRGIAERM
                     IESFMLAANETVAEHYARLKLPFIYRIHEEPKAEKLQKFIDYASVFGVQIQGTATKIT
                     QSALQDFMKKVQGQPGSEVLSMMLLRSMQQARYSEHNHGHYGLAAEYYTHFTSPIRRY
                     PDLLVHRMIRDYDDKAMDKADHFANLIPEIATQTSSLERRAIDAERIVEAMKKAEYME
                     EYVGEEFEGVVASVVKFGMFVELPNTIEGLIHVTTLPEYYHFNERTLTLQGEKSGKVF
                     RVGQQIKVKLIRSDKETGDIDFDYLPSDFDIVEKVSKSSREGRPNRSSKREHQHRISD
                     RDNKNKNTSKKKASRKPKRNSDSKSHHHKDDRTTGSTKKKTKKPFYKGVAKKGQKRKS
                     KKG"
     gene            4435..4902
                     /gene="smpB"
                     /locus_tag="ACUBV3_001384"
     CDS             4435..4902
                     /gene="smpB"
                     /locus_tag="ACUBV3_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HHU3847882.1"
                     /translation="MVKGQGNVVAQNKKAHHDYTIVETIEAGIVLTGTEIKSVRAARI
                     TLKDGYAQIKNGEAWLINVHITPYDQGNIWNQDPDRTRKLLLKKREIEKISNELKGTG
                     MTLVPLKVYLKDGFAKVLLGLAKGKHDYDKRESIKRREQNRDIARQLKNYNSR"
     gene            complement(4986..6374)
                     /locus_tag="ACUBV3_001385"
     CDS             complement(4986..6374)
                     /locus_tag="ACUBV3_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HHU3847883.1"
                     /translation="MSKNNNDHTQKSENGMVRGLENRHVQLIAIAGTIGTGLFLGAGR
                     SISLTGPSIVLVYAITGAFMFFMMRAIGEMLYQDPNQHTFINFITKYLGSGWGFFSTL
                     SYWISLIFIGMAEITAVGEYVQFWFPEWPSWIIQIVFLAILSSINLIAVKAFGETEFW
                     FAMIKVIAILGLIATGIFMVLTNFDTGHGYHASISNITNHFEWFPKGKLNFFMAFQMV
                     FFAYLAIEFVGVTTSETANPRKVLPKAIQEIPMRIILFYAGSLLAIMAIFPWQQLPVN
                     ESPFVTVFKLAGIKWAAALINFVVLTSAASALNSTLYSTGRHLFQLANESPNALTKAL
                     KLDQLSRQSVPSRAIIASAVIVGASALISVLPGISDAFSLITASSSGVYISIYVLIMI
                     AHWKYRKSPDFMEDGYKMPAYKILSPITLLFFLFVFVSLFLQDSTYIGAIGATIWIIG
                     FGLYSHFKHKSH"
     gene            complement(6503..7429)
                     /locus_tag="ACUBV3_001386"
     CDS             complement(6503..7429)
                     /locus_tag="ACUBV3_001386"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HHU3847884.1"
                     /translation="MALLSVIVPCYNEQETVSTFLTEIKKVESEMARYTHFEYIFVND
                     GSTDRTLELLKKAAKQFDNVHYLSFSRHFGKDAALLAGLEHTTGDFITVMDVDLQDPP
                     TLLPEMYLKLQEGYDIVATRRKDRKGEPLIRSLFAKLFYKLINQVSDTKMVDGARDFR
                     LMTKQVVDSILELNEVNRFSKGIFSWIGYDVAYISYENRERIAGKTSWSFFNLLKYSL
                     DGFINFSEIPLAIATWIGTLSSVLSLLAIIFIIIRKLLFGDPVSGWASTVTIVLFMGG
                     IQLLSLGIIGKYISKIFLETKKRPVYIVKEEG"
     gene            complement(7558..7950)
                     /gene="gloA"
                     /locus_tag="ACUBV3_001387"
                     /gene_synonym="lguL"
     CDS             complement(7558..7950)
                     /gene="gloA"
                     /locus_tag="ACUBV3_001387"
                     /gene_synonym="lguL"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HHU3847885.1"
                     /translation="MPFLHTCIRVKDLDASIAFYQEALGFKEVRRNDFPENQFTLVYM
                     SLEDDPSYELELTYNYDHEAYDLGNGYGHIAVGVDDLETTYDAHQKAGYSVTKISGLP
                     GKPNMFYFIQDPDGYKIEVIRLSQFKAD"
     gene            complement(8038..8712)
                     /locus_tag="ACUBV3_001388"
     CDS             complement(8038..8712)
                     /locus_tag="ACUBV3_001388"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HHU3847886.1"
                     /translation="MTKEDIKKQVRRAFDFRMAIRVYNNNDIPKEDMEYILDTAWLSP
                     SSVGLEGWRFLVLDRQTIAKFRDKLKEVAWGAQYQLDTASHFVLLLAEKGAYYNADSM
                     INSLIRRGLGDPAALESRISLYKSFQENDMKIDSERSLWDWTAKQTYIALGNMMTAAA
                     MIGVDSCPIEGFDYEKVNNILSKEGLIDDKKEAISCMVSFGYRLREPKHSRARKERQE
                     VITWVE"
     gene            8979..9821
                     /locus_tag="ACUBV3_001389"
     CDS             8979..9821
                     /locus_tag="ACUBV3_001389"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HHU3847887.1"
                     /translation="METYTLSNTLNIPKIGFGTWQLTEGEEAYKAVTHALKVGYTHID
                     TAQIYGNEHSVGRAIRDSGLARESIFLTTKIWNDKHDYHLAKASIDESLQKLGVDYID
                     LLLIHWPNPKALRENDAWKAGNAGTWKAMEEAYKEGKVKAIGVSNFMKHHLEALFETA
                     EIKPMVNQIILAPGCAQEDLVRFCKGNDILLEAYSPFGTGTIFENESIKAIAEKYGKS
                     VAQVALRWSLDNGFLPLPKSATPKNIEANLDIFDFQLNEDDIATLIQLDSGIKPKDPD
                     NVSF"
     gene            10012..10734
                     /locus_tag="ACUBV3_001390"
     CDS             10012..10734
                     /locus_tag="ACUBV3_001390"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HHU3847888.1"
                     /translation="MRLDKFLVECGLGSRTQVKLILKKKQISVNSNSETSPKVQVDEY
                     RDEIKYNGTLVSYEKFVYYMLHKPKGVISATDDPSHKTVLDLLDKTARDKAVFPVGRL
                     DIDTTGLLLITNNGELAHKMLSPKKHVDKCYEAKISGIMTEDDILAFDKGIILKDFTC
                     LPALLEIVEVNQVKKQSLVKITIKEGKFHQVKRMVAACGKEVLELKRLSMGNLQLDKQ
                     LESGQWRRLTIKEIEKLEKHMQ"
BASE COUNT         3672 a         1707 c         2076 g         3286 t
ORIGIN      
        1 tttactaaat ctataacgat gccaatatta ttagtaagcc taggaattat agttgttatt
       61 ttaattattg gctatttagc agttgtagta tttgctccaa aaactttccg tttaaaaagt
      121 taattttaat tgtggtataa taacagaggc atgtgataac ataggtttag ttacataaag
      181 gaaattataa tggaaatagc atctcaaata gatgttaaat ctcaataaaa ctgctaagat
      241 agttagtggt agaaaagttt agaaagaaga tgatgtttta tctcagttga aacggttaac
      301 tggaagcaaa atttaaaagt ggcttggctc ggtaattttt ttactggggc aagcttttca
      361 cttgttatgc cttttatggt attatatgta gaacaattag gagcacctag caacaaagta
      421 gagtggtatg caggcctatc agtatcctta tcagccttgt cttcagcttt agttgcccca
      481 ttatgggggc gcttagctga taaatatggt agaaaaccga tgatggtaag agcagggttg
      541 atgatgactt ttactatggg aggccttgct ttcattcata gtgttacagg acttctgatt
      601 ttacgtatat taaacggcat ttttgcaggc tatgtaccta actctacagc tttgatagca
      661 agtcaagctc ctcaagagga atcaggatat gccttaggaa ccttagcgac aggagtgact
      721 ggaggtatgt taattggtcc acttttaggg ggacttttag cagagtggtt tggtattagg
      781 gaagtttttt tattagtggg aaccatattg ttaatatcta ctttaatgac catttttatg
      841 gtaaaggaag atttcaaacc catttctaac gaagaaacca tgccaactac agaagtgttc
      901 aaaagcgtta aaagcttaca aattttaata gggctgtttg taacaagcat gattattcaa
      961 atttctgcac aatcaattgc acctatttta acactttaca ttcgtcattt aggacagact
     1021 gaaaacttga tgtttgtttc cggattaatt gtttctggta tgggattctc tagtattctg
     1081 agtagtccaa agctaggaag aatcggggat cgcataggca atcatcgttt gctattatta
     1141 gctctactgt atagtttcct catgtatgtc ttatgttcat tggcacagac ttcattacag
     1201 ctaggggtta ttagattcct gtatggtttt ggaacaggtg ccttgatgcc gagtattaat
     1261 tcaatcttga ctaaaattgc tccaagacaa ggtttatcaa ggattttcag ttataaccaa
     1321 atgtttagca atttaggaca agtattaggt ccttttgtcg gttcagcagt ttctattcat
     1381 ttaggattcc gatgggtttt ctttgtgact agcttcatcg ttttagctaa ttttgtttgg
     1441 tgttttatta attttagaaa atatattaga gttaaggaaa ttgtgtgaga gttaaaatta
     1501 atttaaagtg tagctcttgt ggtagtaaga attatttaac cagtaagaat aaaagtaatc
     1561 atcccgataa aatcgaggtt ccaaagtatt gcccaaagga gagaaaagta acactacata
     1621 ttgaatctta ataagtttta tgttagaatg tactttgacc atagtggagg aattaacatg
     1681 tataacctat tactcaccat tctacttgtt ttatcagtat tattgattat ctcaattttc
     1741 atgcaaccac aaaagaatcc aagtagtaat gtttttgata gtagcggttc tgaagccctt
     1801 tttgaacgta gtaaagctcg aggatttgaa gcttttatgc aacgttttac aggtgtttta
     1861 gtctttttct ggctactaat cggcttagta ttatctattt tatcaagtca ttaatgatag
     1921 ctaaatacag ataagtgagg atgggggata gcccaacctc actttttgtt agtagaatgg
     1981 ttgtagagta ataataaagt gactaaagtt actagaaaag gaaataatga aagaaaaaat
     2041 tataaaatat ttggaagaac acggtaaaac cagcgttaat gatttagctg ctgccctaga
     2101 tatggcaggc gcaaaagcat ttccaaaatt aatcaaaaca atctcaaatt tggagagcca
     2161 caggcaatta agatttgacg ataatggaag tctttctctt caaaagaaag aagctaagaa
     2221 aaaggaaatc acagtaaggg gtctttttag agcaaataaa gcaggatttg gttttcttag
     2281 tattgaccaa gacgaagatg atatgtttat cggaaaaaac gatattgctt atgctattga
     2341 tggtgacacg gtagaagctg ttgtaaagaa accagcggac cgtcttaacg ggactgcagc
     2401 agaagcgcgt gttgttaata ttgttgaacg tagtttaaag acgcttgttg gtaagtttgt
     2461 cttagacgat gagcgcccta aatacgcagg atatatcaaa tcgaaaaatc aaaaaatcaa
     2521 tcaaaagatt tatatacgca aagaaccggt cgttttagac ggaacagaaa ttatcaaagt
     2581 agatattgat aaatatccga cacgaggaca tgattacttc gtagcaagtg ttcgtgatat
     2641 cgttggacat caaggagatg ttggtattga tgttttagaa gtcttggaaa gtatggatat
     2701 cgtctcagaa tttcctgagg acgttattgc agaagccaat gctatcccag atgctccaac
     2761 ggagaaagat ttaattgggc gtgttgattt acgtcaagag gtcactttca ctattgatgg
     2821 agcggacgcc aaagatttag acgatgctgt gcatattaaa ttactggata atggtcattt
     2881 tgaacttgga gttcatatcg cagatgtatc atactacgtt actgaaggct ctgccttaaa
     2941 tcgtgaagca cttagtcgtg gtacgtccgt atatgtcaca gatcgcgttg taccaatgtt
     3001 accagagcgt ctatctaacg gtatatgttc attaaatcca aatcttgatc gtttaacgca
     3061 gtcttgtatt atggaaattg accaaaatgg gagagttgtt aatcatcaaa ttacacagtc
     3121 agtgattaat accacttatc gtatgactta tatcgctgta aatgatatta ttgctggcga
     3181 tgaggaaatc tgctcagaat atgagtctat tgtgtcctca gtacagcata tggtcacttt
     3241 acaccatact ttggaagcta tgcgtacgcg tcgtggagcg ctgaattttg acacatctga
     3301 agcaaaaatt atggttaatg ataagggaat gccagttgat attgttattc gcaacagagg
     3361 cattgctgaa cgcatgattg agtcatttat gttggcggca aatgaaacgg ttgctgaaca
     3421 ttatgctcgt ctaaaactgc catttattta tcgtatacac gaagagccta aagctgaaaa
     3481 attacaaaaa ttcatcgatt atgcttctgt tttcggtgtt cagattcaag gaacagcaac
     3541 taaaatcacc caatctgctt tacaggactt tatgaaaaaa gtacaagggc agccaggaag
     3601 tgaagtgtta tctatgatgt tattgcgctc gatgcaacaa gctagatatt ctgaacacaa
     3661 ccatggacat tatggacttg ctgcggaata ttacactcac tttactagcc ccattcgacg
     3721 ttatccagac ttgttagttc atcgtatgat tcgtgattat gatgataaag cgatggataa
     3781 agctgatcat tttgctaacc ttatcccaga aattgcaact caaacgtcta gtcttgagag
     3841 acgtgccatt gatgctgaac gtattgtaga ggcaatgaag aaagccgaat atatggaaga
     3901 atatgttggt gaggaatttg aaggtgtggt tgcaagcgtt gttaaatttg gtatgtttgt
     3961 agaattacca aatactattg aaggattgat tcacgtaacg accttgcctg aatattatca
     4021 ctttaatgaa cgtacgctga cgcttcaagg tgagaaatct gggaaagttt tccgtgttgg
     4081 tcaacaaatt aaggttaagt tgattcgttc tgataaggaa actggtgata tcgattttga
     4141 ttatttacca tcagattttg atattgttga aaaggtaagt aaaagctcgc gtgagggtcg
     4201 tcctaataga agttctaaac gagaacatca gcatagaatt tctgatcgtg ataataagaa
     4261 taaaaataca tctaagaaaa aagcatctcg aaaacccaaa aggaactcgg atagcaaatc
     4321 acatcatcat aaagacgata gaacaacagg ttcaaccaag aaaaaaacga aaaaaccttt
     4381 ctataaaggc gttgctaaga aaggtcagaa acggaaatct aagaaaggtt aagtatggtt
     4441 aaaggtcaag gaaacgttgt agcacaaaat aaaaaagcgc atcacgacta tactatcgtt
     4501 gaaacaatag aagcaggaat tgttttgaca ggaacagaaa tcaaaagtgt ccgcgcagcg
     4561 cgtattacat tgaaagatgg atatgcacaa ataaaaaatg gtgaagcgtg gttgattaat
     4621 gttcatatta caccatatga tcaaggtaac atatggaatc aagatcctga tcgtacacga
     4681 aagttattac taaaaaaacg tgagattgaa aaaatatcaa atgaactgaa agggactggt
     4741 atgactctgg tacctctaaa agtttactta aaagatggtt ttgctaaagt tctattgggt
     4801 ttggcaaaag gaaaacatga ttatgataag cgagagtcta tcaaacgtcg tgagcaaaac
     4861 cgtgatattg cccgtcagtt aaaaaactat aattcacgat gattatcaaa aggttaggga
     4921 attgcctaac cttttttgga cgaaaaaagt gaagttgatt gaccaacttc actaataata
     4981 tagacttaat gtgatttatg tttgaagtga ctatataaac caaatccaat aatccagatg
     5041 gttgctccaa tagccccgat gtatgttgaa tcttgcaaga agagagaaac gaatacaaag
     5101 aggaagaaga gaagtgtgat tggtgaaagg attttgtagg caggcatttt ataaccatct
     5161 tccataaaat caggtgattt acgatatttc caatgtgcaa tcataataag aacatagata
     5221 gaaatgtaaa caccagaaga tgatgcagta attaaggaga aggcatctga aataccaggt
     5281 aaaacactga taagtgctga agctccaacg attacagctg atgcaataat agctcgactt
     5341 ggaacgcttt gacgagataa ttggtctaat tttaacgctt ttgtaagagc atttggtgat
     5401 tcattagcca attggaataa atgacgccct gttgagtaca aagtagaatt aagagcagat
     5461 gctgcagacg ttaggacaac aaagttaatc aaagcggcag cccacttaat cccagctagt
     5521 ttgaatactg taacgaaagg tgactcatta actggtaact gttgccatgg gaagatggcc
     5581 ataattgcta ataatgaacc tgcgtagaat aaaataatac gcatcggaat ttcttggatt
     5641 gcttttggta aaactttacg tggattagct gtttcagaag ttgttactcc gacaaattca
     5701 atagctaaat aagcaaagaa taccatttgg aaagccatga agaaattcaa tttaccttta
     5761 gggaaccatt caaaatgatt ggtgatattt gagattgaag catggtatcc gtgaccggtg
     5821 tcaaagtttg tcaataccat aaagataccg gttgctatta aaccaagaat tgcaataact
     5881 tttatcatag caaaccaaaa ctcggtttct ccgaatgctt ttacagcaat taagttgata
     5941 gaacttagga tagctaaaaa aacaatttgg ataatccagg atggccattc agggaaccaa
     6001 aattgaacat actctcctac ggctgtaatc tctgccatac cgataaatat tagggaaatc
     6061 caataagaaa gtgttgagaa aaatccccaa ccggatccta aatatttggt aatgaaatta
     6121 ataaaagtat gctgatttgg atcttgataa agcatctctc caatagcacg catcatgaag
     6181 aacatgaaag cgccagtgat agcatagaca agaactattg aaggacccgt caggctaatt
     6241 gaacgacctg caccaagaaa aagcccagtt ccgattgtcc ctgcaatcgc aattaactgt
     6301 acgtgacgat tttctaggcc gcgtaccata ccattctcag atttttgagt gtggtcatta
     6361 ttattttttg acattataaa tactccatct atttttgaaa aactccttca ttatataatt
     6421 tttttgcaaa attcgcaata attactatac aatatttttc tatgataaaa tctcctagct
     6481 aagtgagcta agagatttca attcatccct cttccttgac aatatagact gggcgttttt
     6541 tagtttctag gaaaatctta gagatgtatt tgcctataat tcctagcgac aataactgaa
     6601 taccacccat aaaaagtaca atggtaactg tgcttgccca acctgaaacg ggatccccaa
     6661 atagtaattt tcgaattata ataaaaatga tagctaaaag tgatagtact gaactaaggg
     6721 ttccaatcca ggtagctata gctaatggaa tttctgagaa gttgatgaaa ccgtctagag
     6781 agtattttaa gagattaaag aaactccatg aggtcttacc cgcaattcgt tctcgattct
     6841 cataactaat ataagcgaca tcatatccaa tccaggaaaa aatccctttt gagaagcgat
     6901 tcacttcatt tagctcgaga atactgtcaa caacttgttt tgtcatgaga cgaaaatcac
     6961 gcgcaccatc aaccatttta gtatctgaca cttgattgat taatttataa aaaagcttgg
     7021 caaaaagaga gcgtatcaac ggctcccctt tacgatcttt tcgccttgta gcgacaatat
     7081 cataaccttc ttgaagtttt aggtacattt ctggtaataa agtgggtgga tcttgcaagt
     7141 caacatccat aacagtgata aaatcaccag tagtatgttc taaaccagcc aataaagcag
     7201 catctttacc aaaatggcgt gaaaatgaaa ggtaatgaac attgtcaaat tgtttagctg
     7261 ctttcttaag aagttcgagt gtacgatcgg tagaaccatc gtttacgaaa atatattcaa
     7321 aatgggtgta acgagccatt tcggattcaa cttttttgat ttctgttaaa aaagttgaga
     7381 cagtctcttg ttcattataa caagggacaa taactgataa taaagccatt tgtaattctc
     7441 cgtatcattc ttgcttaaat tttaacataa tatgggaaaa aatagaaaga ttataaggta
     7501 atcataaaaa ggactaggtt tttgcctcag tccttaactt aatatgatga gatgttatta
     7561 gtcagcttta aattgactaa gtcgaataac ttcaatttta tagccatctg gatcttgaat
     7621 aaagtagaac atattaggtt tacctggtaa tccagaaatt tttgttacag agtaacctgc
     7681 tttctgatgg gcatcataag tagtttccaa atcgtctaca ccaacagcaa tgtgaccata
     7741 cccatttcct aaatcataag cttcgtgatc atagttatag gttaactcta attcgtaact
     7801 agggtcgtcc tctagggaca tatagactaa agtaaattga ttttcaggaa agtcattacg
     7861 acgaacttcc ttaaaaccaa gtgcttcttg ataaaaagca attgaggcgt ccaaatcttt
     7921 aacgcgaata caagtatgta aaaatggcat agtctctctc ctatttcttt attaaacgta
     7981 ttatgtcaaa ataaaattat cttttcaaaa atattgatta taaattaggc tgtctattta
     8041 ctcaacccaa gtaatgacct cttgacgttc tttcctagca cgggaatgtt taggttcacg
     8101 caaacgataa ccaaatgaaa ccatacaaga aatagcttct ttcttatcgt ctattaaccc
     8161 ttctttacta agaatattat tgaccttttc atagtcaaat ccttcaattg ggcatgaatc
     8221 tacgccgatc atagcagcag ccgtcatcat attaccaagt gcgatataag tttgttttgc
     8281 tgtccaatcc caaagagaac gttcagaatc aattttcata tcgttttctt ggaaggattt
     8341 gtagagagat atcctactct caagggcggc tggatcacct agaccacgtc gaatgagact
     8401 gttaatcatg ctatcagcgt tataataagc acctttttca gctagtagta aaacaaagtg
     8461 actagcagta tctagttgat attgtgctcc ccaagcaact tccttaagtt tatctcgaaa
     8521 ttttgcaatt gtttgtctat ctagtactaa aaatcgccag ccttctaaac cgactgaaga
     8581 aggactcaac caagcagtgt ctaaaatata ctccatatct tctttaggaa tatcattatt
     8641 attataaaca cgaatcgcca tacgaaaatc aaaagctctt cgaacttgtt ttttgatatc
     8701 ttcttttgtc attttagatc ctcctgttat taatgctatc atatcacaat ttagaaaaaa
     8761 tctcagcagg cggtatcgtg gcaaagtaga gctacaaaat tctaatgact gtcagaaaaa
     8821 taaagggaat gaacaaaatg atagggtttt gatgaagaaa gttatataat tttacataag
     8881 gaggggataa taccatatat caaactatca tttagatgtt taaaatagat gtttttcact
     8941 atgaaattca tttttgtcac atagaaaagg agattgttat ggaaacatat actctatcga
     9001 atactcttaa cattcctaaa attggttttg ggacatggca actaactgaa ggtgaagaag
     9061 cttataaagc agtgactcat gctttaaaag ttggttatac tcatattgat actgctcaga
     9121 tttatggtaa tgaacatagt gttggacgtg ctataagaga cagtggtttg gcgcgagaaa
     9181 gtatcttttt gacaactaag atttggaatg acaaacatga ctatcattta gccaaagctt
     9241 ctattgatga atctctgcag aaattaggtg ttgattatat tgatcttctt ttgattcatt
     9301 ggccaaatcc aaaagctctt cgtgaaaatg atgcgtggaa agctgggaat gcaggcactt
     9361 ggaaagcgat ggaagaagct tataaagaag gaaaggtaaa agctattggt gtttcgaatt
     9421 tcatgaaaca tcacttagag gctttatttg aaaccgccga aataaaacct atggttaatc
     9481 aaattatcct agcaccaggt tgtgctcaag aagatttggt tcgcttctgt aaaggaaatg
     9541 atatcttatt agaagcttac agtccatttg gaacaggtac tatttttgaa aatgaatcta
     9601 taaaagctat tgctgaaaaa tatggtaaat cagttgcaca agtagctctt cgctggtcac
     9661 ttgataatgg ctttttacca ttacctaagt cagcgactcc caaaaatatt gaagcaaacc
     9721 ttgatatttt tgattttcaa ttaaacgaag atgatattgc taccttaata caactagatt
     9781 caggtattaa acccaaagat ccagataatg tctcatttta ataagaaaag atggaaatgt
     9841 atttccatct tttttatgtg ggatagttcc ttaaagttaa gaaaaacatt aagacctcca
     9901 ttgacgagga gaatatatta atgatagtat tactagaaaa gtagtaataa aggatgagta
     9961 tggtaaaatc ctttacaata tatcattatc gaaagaggtt ataacatcta aatgagactt
    10021 gataaatttt tagtagaatg cggacttggt tcacgtactc aagtcaaatt aatattgaaa
    10081 aaaaagcaga ttagtgttaa tagtaatagc gagacatctc caaaagtaca ggtagatgaa
    10141 tatagagatg agattaaata taatggaact ctagtgtcat atgaaaagtt tgtttattat
    10201 atgcttcata aacccaaagg tgttatatca gcaacggatg atccttcgca taaaaccgta
    10261 ttagatttgc tagacaaaac tgctcgagat aaagctgttt ttccagtggg gcgtttagat
    10321 attgatacaa caggcctttt gttaataact aataatgggg aattagcgca taaaatgtta
    10381 tcacctaaaa aacacgttga taaatgctat gaggcaaaaa tctcagggat aatgacagaa
    10441 gacgatattt tggcatttga taaagggatt attctgaaag attttacttg tttaccagca
    10501 ttattagaaa ttgtggaagt aaatcaggtg aagaagcaga gtcttgttaa aatcactatt
    10561 aaggaaggaa aattccatca agttaaacgt atggttgctg cgtgtggcaa ggaagtctta
    10621 gagctaaagc gtctaagtat gggaaatcta caattagata aacagctaga aagtggacaa
    10681 tggagacggc taacaataaa agaaatagaa aaattagaaa aacatatgca ataatataaa
    10741 a
//
LOCUS       DBJYSH010000070        10517 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.070, whole genome shotgun sequence.
ACCESSION   DBJYSH010000070 DBJYSH010000000
VERSION     DBJYSH010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 10517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10517
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.070"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            63..1049
                     /gene="dhaQ"
                     /locus_tag="ACUBV3_001391"
     CDS             63..1049
                     /gene="dhaQ"
                     /locus_tag="ACUBV3_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DhaKLM operon coactivator DhaQ"
                     /protein_id="HHU3847889.1"
                     /translation="MRKTEHHKMEYVFETIKAITKTNPKVEQIKDLPAIHYLGQSEQV
                     IPILSGGGSGHEPAHFGYVGEGMLSAAISGPIFVPPCASDILETIRFINRGKGVFVII
                     KNFEADLEEFSQAIEQARQEGIPIKYIVSHDDISVETSNFKIRHRGVAGTVLLHKIIG
                     QAALEGASLDELEQLGLSLTTSMATLGVASKSATILGQHQPVFDLEEGYISFGIGIHG
                     EPGYRTMPFVSMEHLANELVNKLKMKLRWQDGEAFILLINNLGGSSKMEELLFTNAVM
                     EFLALDDLQLPFIKTGHLITSLDMAGLSVTLCRVKDSRWIDYLKHKTDARAW"
     gene            1167..1883
                     /locus_tag="ACUBV3_001392"
     CDS             1167..1883
                     /locus_tag="ACUBV3_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008088325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HHU3847890.1"
                     /translation="MGRKWANIVAKKTAKDGANSKVYAKFGVEIYVAAKQGEPDPESN
                     SALKFVLDRAKQAQVPKHVIDKAIDKAKGNTDETFVEGRYEGFGPNGSMIIVDTLTSN
                     VNRTAANVRTAYGKNGGNMGASGSVSYLFDKKGVIVFAGDDADTVFEQLLEADVDVDD
                     VEAEEGTITVYTAPTDLHKGIQALRDNGVEEFQVTELEIIPQSEVVLEGDDLETFEKL
                     IDALESDDDVQKVYHNVADF"
     gene            2038..2727
                     /locus_tag="ACUBV3_001393"
     CDS             2038..2727
                     /locus_tag="ACUBV3_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HHU3847891.1"
                     /translation="MLTKPIIGITGNEREMSDIPGYYYDSVSRHISEGVKNAGGLPVI
                     LPISEAESAKAYVEMIDKLIISGGQNVLPSYYGEEKIIESDDYSLARDIFEFALVDEA
                     LKQNKPIFAICRGMQLVNVALGGTLNQSIDNHYQEPYIGFAHYLNVEKGSFLEGFISG
                     DFRINSLHRQSVKLLAEGLIVSARDPRDGTVEAYESRTEQCIIGVQWHPELMLHQIEN
                     QTLFGYFVNET"
     gene            2829..3662
                     /locus_tag="ACUBV3_001394"
     CDS             2829..3662
                     /locus_tag="ACUBV3_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HHU3847892.1"
                     /translation="MTIKKVLSVTGIILVTVASLAACSSKSHTTKTGKKEVNFATVGT
                     TAPFSYVKDGKLTGFDIEVAKAVFKGSDNYKVTFKKTEWSSVFTGIDSGKFQMGGNNI
                     SYSSERSQKYLFSYPIGSTPSVLAVPKNSNIKAYNDITGHKTQVVQGTTTAKQLENFN
                     KKHQKNPVTLKYTNENITQILTNLSDGKADFKLFDGLTVNAIIKNQGLTNLKTIPLTM
                     RDQPYIYFIFGQDQKDLQKYVNNCLKQLRKDGTLSKIAKEYLGGDYVPNEKDLVTPKE
                     K"
     gene            3803..4627
                     /locus_tag="ACUBV3_001395"
     CDS             3803..4627
                     /locus_tag="ACUBV3_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018543698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HHU3847893.1"
                     /translation="MKLKHIVLGLALTTLLGVTFSNQEVSASSTSSKVVKVGVMTFSD
                     TEKARWDKIEKLVGDKAKIKFTEFTDYTQPNQATANKDVDINAFQHYNFLENWNKENK
                     KNLIPLEKTYLAPIRIYSEKVKSLKKLKKGATIAIPNDATNGSRALYVLQSAGLIKLN
                     VSGKKVATVANITSNKKDINIQELDASQTPRALKDVDAAIINNTYIEQANLKPSDAIF
                     VEKSDKNSKQWINIIAGRKNWKKQKNAKAIQAILDAYHTDEVKKVIKDTSADIPQW"
     gene            4761..6137
                     /locus_tag="ACUBV3_001396"
     CDS             4761..6137
                     /locus_tag="ACUBV3_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HHU3847894.1"
                     /translation="MTLTKEENQIQKFLSDTIVQDDINILRDLIAIKSIFAQKVGLND
                     LASYLGEVFRKAGAEVIIDDSYSAPFIVANFKSSKVDAKRIIFYNHYDTVPADEVEQW
                     TEDPFTLSLRYGKMYGRGVDDDKGHITARLSAVKKYLSRHKGELPLDITFIVEGAEES
                     ASVGLDYYLEKYQEQLQGADLIVWEDGPKNPKGQLEIAGGNKGIVTFDLSVSSADVDI
                     HSSFGGVVDSSTWYLIQALNTLRDNKGHILVEGIYDKVIPPTKRELELVEKYSYRSAK
                     ALEDAYQLVLPSLADSHKTFLRKLYFEPSIAIEGITSGYQGEGVKTILPAYAKCKAEV
                     RLVPGLTPKGVLDSIQNHLKENGFNDIELTYTLGEMSYRSDMSAPSILKVVDLAEQFY
                     PEGVSLLPTSPGTGPMYLVHQALRAPIAAFGIGHANSRDHGVDENVSIADYYTHIELV
                     EALIESYE"
     gene            6130..7200
                     /locus_tag="ACUBV3_001397"
     CDS             6130..7200
                     /locus_tag="ACUBV3_001397"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HHU3847895.1"
                     /translation="MSKEPIIKLDNIDVTFHQKKREINAVKDVTIHINQGDIYGIVGY
                     SGAGKSTLVRVINLLQEPSAGKITIDDQVIYDNKVTLTSTQLREQRREIGMIFQHFNL
                     MSQLTAEQNVAFALKHSGLSKEAKAAKVAKLLELVGLSDRAQNYPSQLSGGQKQRVAI
                     ARALANDPKILISDESTSALDPKTTKQILALLQDLNKKLGLTIVLITHEMQIVKDIAN
                     RVAVMQNGKLIEEGSVLDIFSHPRESLTQDFIKIATGIDEAMLKIEQQEVVKNLPVGS
                     KLVQLKYAGHSTDEPLLNQIYKEFEVTANILYGNIEILDGIPVGEMVVILSGDEEKLR
                     QACQAITDSQVQLTLLKEGGKA"
     gene            7197..7889
                     /locus_tag="ACUBV3_001398"
     CDS             7197..7889
                     /locus_tag="ACUBV3_001398"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000574038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HHU3847896.1"
                     /translation="MIEWIQTHLPNVYQMGWEGAYGWQTAIVQTLYMTFWSFLIGGLM
                     GLLGGLFLVLTSPRGVIANKLVFGVLDKVVSVFRALPFIILLALIAPVTRVIVGTTLG
                     SPAALVPLSLAVFPFFARQVQVVLAELDGGVIEAAQASGGTLWDIIVVYLREGLPDLI
                     RVSTVTLISLVGETAMAGAIGAGGLGSVAITKGYNYSRDDITLVATILILLLIFFIQF
                     LGDFLTRRLSHK"
     gene            complement(7938..8993)
                     /locus_tag="ACUBV3_001399"
     CDS             complement(7938..8993)
                     /locus_tag="ACUBV3_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HHU3847897.1"
                     /translation="MRAATYLSANELSLTDKAKPQVIKPTDAVVRLVKTTICGTDLHI
                     LGGDVPACKPGTTLGHEGIGIVEEVGSAVNHFKVGDKVIISCVTACNTCYYCKHQLPS
                     HCEDGGWILGHLIEGTQAEYVHIPHADGSLYHAPEGVCDDALVMLSDILPTSYEIGVL
                     PSHIKPGDTVCIVGAGPIGLSALLTAQFYSPAKIIMVDLSQKRLEASKKFGATHTILS
                     TSTQEVKEEIDKITKGRGVDVVLECVGYPATFDICQNVVSIGGHIANVGVHGKPVEFN
                     LQDLWIKNITLNTGLVNANTTEMLLEVLETGKIDATQLVTHHFKLSEIEEAYKVFKAA
                     EENNTLKVIIENDITPA"
     gene            complement(9107..10474)
                     /gene="brnQ"
                     /locus_tag="ACUBV3_001400"
     CDS             complement(9107..10474)
                     /gene="brnQ"
                     /locus_tag="ACUBV3_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HHU3847898.1"
                     /translation="MKLKNTYIIIGLMLFALFFGAGNLIYPAYLGIYSGSNWAWAIIG
                     FCLTGVTLPLLGVAAVAYSGATDVESFTKPVSKTYAIFFAVALYLSIGPFFAIPRTGA
                     TSFSVGIQPILGDSSLGKIIYGLIFFGISYFLAIRPSQIVDRIGKYLTPFLLTVISIL
                     IIASLIHPAAIVGTPHNASTNVSDAFKDLPFLAGLIQGYGTMDALASIAFAIIVIQAS
                     KQYGAITKKEITSMALKSGAIATFLLAFIYIFVGRIGATSQSLFKFANGSFLLHNTPI
                     DGGHVLSQSANFYLGIVGQAILGTAIFLACLTTATGLITACAEYFHKLLPKISHITWA
                     TIFTLIAITFYFGGLSEIIRWSLPVLYLLYPLTIVLIFLVFFDQKFESSCIVYQTSIA
                     ATAVAALYDALSKLGEMTGLFTIPSALTTFFTKVVPLGEYSMGWISFAICGVLVGLIL
                     KKVKA"
BASE COUNT         3601 a         1665 c         2072 g         3179 t
ORIGIN      
        1 ttaataggaa agtcattgaa aaaattatca gaacatcaat agaaaattat taggacaaag
       61 atatgagaaa aacagaacat cataaaatgg aatatgtttt tgaaacaata aaagcaatca
      121 ctaaaactaa ccctaaagta gagcaaataa aagatttgcc cgctattcat tatttagggc
      181 aatccgaaca agtaattccg attttatcag gaggaggcag cggacatgag ccagctcact
      241 ttggatatgt tggtgaggga atgctatcgg cagcgattag tggtcctatt tttgtaccac
      301 catgtgctag tgatattcta gagactatta gatttatcaa tcgaggcaaa ggtgtttttg
      361 ttattatcaa aaatttcgaa gctgatttgg aagaattttc ccaagcgatt gaacaggctc
      421 gtcaagaagg cattccaatc aaatacatcg tttctcatga tgacatctct gttgaaacat
      481 caaattttaa aattcgtcat cgaggcgttg ctggtactgt gttgctacat aaaattattg
      541 gacaagctgc tttagaagga gcaagcttag atgaattaga acagctagga ctatctttga
      601 caacttcaat ggctacttta ggtgtagcaa gtaagtcagc cactatttta ggtcagcatc
      661 aacctgtttt tgatttagaa gaaggttaca tctcatttgg aatcggtatt catggtgagc
      721 caggataccg gacaatgcct ttcgtgtcca tggaacattt ggcaaacgaa ctagttaaca
      781 aattgaaaat gaaattacgt tggcaagatg gagaagcatt cattctattg ataaataatc
      841 ttggaggaag ttctaaaatg gaggaattac tttttacaaa tgctgtcatg gaatttctgg
      901 ctttagacga tttacaatta ccttttatta aaacaggcca cctaattaca agtttagata
      961 tggctggttt atcagttacc ttgtgtcgtg taaaggatag tagatggatt gattacttaa
     1021 aacataagac agatgctaga gcttggtgat agaatttcca ttttaccaat tgctatcaaa
     1081 gtgctataat aatagagtta aaattaagat tgcttgccaa tatgagccag caggttatta
     1141 aaaatgaaat caaaggagat tttccaatgg gacgtaagtg ggccaatatt gttgctaaaa
     1201 agactgctaa agatggtgct aactcaaaag tatacgctaa attcggtgtt gaaatatatg
     1261 ttgctgcaaa gcaaggtgaa ccagaccccg agtcaaactc agctctaaaa ttcgttttgg
     1321 accgtgctaa gcaagcacaa gttccaaagc atgttattga taaagcgatt gataaagcca
     1381 aaggaaacac agatgaaact ttcgtagagg gacgctatga aggttttggt ccaaatggtt
     1441 caatgattat tgtggatact ttgacatcaa atgttaaccg tacggcagca aatgtacgta
     1501 ctgcttacgg taagaacggt ggcaatatgg gagcttcagg atcggtatcc tacttatttg
     1561 ataaaaaagg tgtcatcgtt tttgctggtg atgatgctga cactgtcttc gaacaattac
     1621 ttgaagcgga tgtagacgta gatgatgttg aagcagaaga gggaacaata acagtttata
     1681 ccgccccaac agatcttcat aaaggtatcc aagcacttcg cgataatggt gtagaagaat
     1741 tccaagttac tgaacttgaa attattcctc aatcagaagt agtattggaa ggtgatgacc
     1801 ttgaaacttt tgaaaagctt attgacgcac tcgaatcaga tgatgatgtt caaaaagttt
     1861 atcataatgt agcagatttc taaaaaataa aaccgtagta ttaatactat ggtttttatt
     1921 tttgtgagag ttgataggta ttgttaaaga agtgataggt aaaaaatatt attcaaaaag
     1981 tttttttagg tgtaaaatga taattattag acagttattg gttgatgagg gagcaaaatg
     2041 ttaacaaagc ctattattgg tattacagga aatgaaagag aaatgtcaga tattccaggt
     2101 tattattatg attcggtatc taggcatatt tcggaaggag ttaaaaacgc aggcgggtta
     2161 ccagttatcc taccaataag tgaagcagaa agtgccaaag cttatgttga gatgatagat
     2221 aagctgatca tatctggtgg ccaaaatgtt ttaccatcct attatggtga agagaagata
     2281 attgagagtg atgattattc acttgcgcgt gatatttttg aatttgcatt agtcgacgaa
     2341 gcgttgaaac aaaataaacc aatctttgcg atttgccgtg gcatgcagtt agtaaatgtg
     2401 gcacttggtg gtacacttaa tcaatctatt gataatcact atcaagagcc ttacattgga
     2461 tttgctcatt atttaaatgt ggaaaaaggg agttttctag aaggctttat atcaggtgat
     2521 tttaggatca attctcttca tagacaaagt gttaaactgt tagctgaagg tctgatagtt
     2581 agtgctagag atcctagaga tgggacagta gaagcttatg aaagtcgaac agaacaatgt
     2641 attataggtg ttcaatggca tccagaatta atgcttcacc aaattgaaaa ccaaacctta
     2701 tttggttatt ttgttaatga aacgtagcat ccatataata gcaaaaactc tcagatgata
     2761 gaagataagt gttagttaaa aacttaaaga tattatgata tcgtgtacaa ttaaagagga
     2821 gaaaaaagat gacaataaaa aaagtgttaa gtgtaacagg aattatttta gtgacagtag
     2881 cgtctctagc tgcttgtagc tcaaaatctc atactaccaa gacgggcaaa aaagaagtta
     2941 attttgcaac tgtcggaaca acggcacctt tttcttatgt aaaggatggg aaactgactg
     3001 gctttgatat tgaagtagcc aaagctgttt ttaaaggttc agataactat aaagtcactt
     3061 ttaaaaaaac agagtggtca tcggtattta ccggtattga ttcaggaaag tttcaaatgg
     3121 gtggaaataa tatttcttat tcatcagaga gatctcaaaa atatttattt tcatacccaa
     3181 taggctctac tccttcagtt ttagcagttc ctaagaatag taatatcaaa gcttataatg
     3241 atattactgg tcataaaaca caggttgtcc aaggaacgac aactgccaag caattagaaa
     3301 atttcaataa aaagcatcag aaaaatcctg ttactctaaa atatactaat gaaaatatta
     3361 cacagattct aacgaatttg agtgatggaa aagctgattt taaacttttt gacggactaa
     3421 cggttaacgc tatcataaag aatcaaggtc tcacgaattt aaaaaccatc cctttgacta
     3481 tgagagatca gccttatatt tatttcatct ttggtcagga tcaaaaagac ttgcaaaagt
     3541 atgtgaataa ttgccttaaa caattacgta aagatgggac actttcaaaa atagctaaag
     3601 aataccttgg aggtgattac gtaccaaatg agaaagatct cgttactcct aaggagaagt
     3661 agtaataaaa aactaaggag atgtcaatcc ttagtttttg ttatatagtt taaaactata
     3721 acaagagaca gtttataaca atttgattct aataacttcc tatgggataa tatctttatc
     3781 acaaaaattg agggaattta ttatgaagtt aaaacatatt gtcttaggat tagccttaac
     3841 aacactttta ggagtcacat ttagtaatca agaagtttca gcaagctcaa cttcaagtaa
     3901 agttgttaaa gttggtgtta tgaccttttc tgacactgaa aaagcacgtt gggataaaat
     3961 tgaaaagcta gtaggcgata aagctaaaat caaattcaca gaatttacag attatacaca
     4021 accaaatcaa gcgacagcca ataaggatgt ggatattaat gcctttcaac attacaattt
     4081 cttagaaaac tggaataagg aaaataagaa aaacttaatt ccacttgaaa agacttactt
     4141 agccccaatt cgtatctatt ctgagaaggt aaaatctctt aaaaaattga aaaaaggagc
     4201 cactattgca attccaaatg atgcaacaaa tggtagccgt gcattgtatg tccttcagtc
     4261 agcaggttta atcaaattga atgtttctgg taagaaggtt gcaacagttg ctaatatcac
     4321 atctaataaa aaagatatta atattcagga gttagatgcg agtcaaacac cacgtgcact
     4381 caaagatgta gatgcagcta ttattaataa tacatacatt gagcaagcta atttaaaacc
     4441 ttcagatgct atctttgttg agaaatcaga taaaaattca aaacaatgga ttaatatcat
     4501 tgcgggacgt aaaaattgga aaaagcaaaa gaacgctaaa gctatccaag ctatcttgga
     4561 tgcttatcac acagatgaag tgaaaaaagt tatcaaagat acttcagctg atattccaca
     4621 atggtaacgt gaattgactg tataagttta acagggaatc ctaataggat acaacttatc
     4681 agccatatag ccataaaggg cctgggacga atacaatgtg ccaggctctt ggattgtttt
     4741 tgaaggaagg tggatgaaag atgactttaa caaaggaaga aaatcaaatt caaaagtttc
     4801 tatctgatac tattgttcag gatgatatca atattttaag agatttaatt gcaattaagt
     4861 caatttttgc tcagaaagtt ggtttaaatg acttagcaag ctatcttgga gaagttttta
     4921 gaaaagctgg cgcagaagtc attatagatg acagctatag cgctcccttt attgtggcaa
     4981 atttcaaatc atcaaaagta gatgctaagc gtattatttt ttacaatcat tatgatactg
     5041 taccagcaga tgaggtagaa cagtggactg aggacccttt tacactttca cttcgatatg
     5101 gtaagatgta tggacgtggt gtagatgatg ataaaggtca cattaccgca cgtttatcag
     5161 cagttaaaaa atatttatca agacacaaag gggaacttcc tctagatatc acttttattg
     5221 tggaaggagc ggaggaatca gcttcagtag gccttgatta ttatttagaa aagtatcaag
     5281 agcagctaca aggagctgat ttgatcgtgt gggaagatgg ccctaaaaat cctaaaggac
     5341 agttggaaat tgctggtgga aataaaggca ttgtcacttt tgatttatca gtaagttcag
     5401 cggatgttga tattcattca tcctttggtg gggttgttga ttcctcgaca tggtatttaa
     5461 ttcaagccct caatacgtta agggataaca aaggtcatat attggtagaa ggtatttatg
     5521 ataaagttat tccccctact aagagagagt tagaattagt tgaaaaatat tcttatagat
     5581 ctgcaaaggc gctagaagat gcttaccaat tagttttgcc cagtttagca gatagtcata
     5641 aaactttttt gagaaagctt tattttgaac cttctatagc cattgaaggt ataacctcag
     5701 gttatcaagg tgaaggcgtc aaaacaattt tacctgctta tgctaaatgt aaagcagagg
     5761 ttaggttagt gccagggtta accccgaaag gtgtactaga ttcaatacaa aatcatttga
     5821 aagagaatgg ctttaatgac attgagctaa cctataccct tggtgaaatg tcttatcgta
     5881 gtgatatgtc agcgccatct atcctaaaag ttgttgattt agctgagcaa ttttatccag
     5941 aaggggtatc tctgttaccc acctcaccag gtacaggacc aatgtattta gttcatcaag
     6001 ctctaagagc gccaattgca gcttttggca taggacatgc caatagtcga gaccatggcg
     6061 ttgatgaaaa tgttagtatt gctgattatt acacccatat tgaattagtg gaggctttaa
     6121 tagaatcata tgagtaaaga accaattatt aaattagaca acattgatgt tactttccac
     6181 caaaagaaac gtgagattaa tgctgttaaa gatgttacta tccatattaa tcaaggtgat
     6241 atatatggaa ttgtcggtta ttcaggagcc ggaaaatcta cccttgttcg tgttattaat
     6301 ttgctacaag aaccttcagc aggtaaaatt actattgatg accaagttat ttacgacaac
     6361 aaggtgacac tgactagcac acaattacgc gaacagcgcc gtgaaattgg aatgattttt
     6421 caacatttta acctaatgtc acagttgaca gcagagcaga atgtagcttt tgctcttaaa
     6481 cattcaggtt tatcaaaaga ggcgaaagca gctaaggttg ctaaattatt agaacttgtt
     6541 ggattatccg atcgtgctca aaactatcct tcacaattat caggagggca aaaacagcgt
     6601 gttgccattg cgcgtgcatt ggctaacgat cctaaaatct taatttcaga tgagtctacc
     6661 tctgcacttg accctaaaac aacaaaacaa atacttgccc ttctccaaga tctgaataaa
     6721 aaattgggac ttacgattgt tttgattaca catgagatgc aaattgtcaa agacattgct
     6781 aatcgcgttg ctgttatgca aaatggtaaa ttgattgaag aaggatcagt tttagacatt
     6841 ttttcacatc ctagagagag tcttacacaa gattttataa aaatagctac tgggattgat
     6901 gaggcaatgc ttaagattga gcaacaagaa gttgtcaaaa atctaccagt aggtagcaaa
     6961 ttagttcaac tgaaatacgc tgggcatagc acggatgagc cgcttttaaa tcaaatttat
     7021 aaagaatttg aagtaactgc taacatcctc tatgggaata ttgaaatttt agatggtatt
     7081 ccagttggag aaatggtagt tatcttatca ggtgacgaag aaaaattaag acaagcctgc
     7141 caagcaatta cagattcgca agttcaatta acgttattga aagaaggtgg taaagcatga
     7201 tagagtggat tcaaacacat ttaccaaatg tatatcaaat gggttgggaa ggtgcttacg
     7261 gctggcagac agctattgta caaacccttt atatgacttt ttggtcgttc cttattggag
     7321 gtttaatggg attgttagga ggtttattcc ttgttttaac tagtcctaga ggagttattg
     7381 ctaataaatt agtatttgga gttttagata aagttgtttc tgtttttaga gctctgccct
     7441 tcattattct tcttgctttg attgcgccag taactcgcgt aattgtagga acaacacttg
     7501 gttcaccagc agctttggta cctctttctt tggcagtttt cccatttttt gctcgtcaag
     7561 ttcaagttgt tttagctgaa cttgatggtg gagttattga ggctgcacaa gcctcaggtg
     7621 gaacactttg ggatattatt gtagtttatc ttcgtgaagg tctaccagat ttaattcgag
     7681 tatcaacggt tactttgatt tctttagtag gtgaaacagc tatggctggc gctattggtg
     7741 caggaggatt gggttctgtt gctattacta aaggatataa ctattctcgt gatgatatta
     7801 ctttagtagc gactattctg attttattat taattttctt tatccaattt ttaggtgatt
     7861 ttttaacacg tcgcttgagt cataaataaa aatgtgagaa ggctattctt ctcacatttt
     7921 catttgttta aaaattatca ggctggagta atatcgtttt cgatgataac tttgagagta
     7981 ttgttttctt cagcagcttt aaatacctta taagcttctt cgatttcaga tagcttgaag
     8041 tgatgtgtta ctagttgtgt tgcatcgatt ttgcctgtct caagaacttc aaggagcatt
     8101 tcagttgtat tagcattaac taatccagta ttaagggtta tattttttat ccataaatct
     8161 tggaggttaa attcaacagg tttaccatga acaccaacat ttgcgatatg tcctccaata
     8221 gaaactacat tttgacaaat atcaaatgtt gcaggataac caacacattc taatacgaca
     8281 tcaacgccac ggcctttggt aattttatca atttcttctt taacttcttg agtagacgtt
     8341 gatagaatgg tgtgtgttgc accaaatttt ttagaggctt caagtcgttt ttgagataaa
     8401 tcaaccataa taattttggc aggagaatag aattgtgcag ttagtaaggc agatagtcca
     8461 attggtccag cgcctacaat acaaacagta tcacctggtt taatgtgaga aggtaagacg
     8521 ccgatttcgt atgaagttgg taaaatatca gataacataa ccaatgcatc gtcacaaaca
     8581 ccttcaggtg catgataaag gctaccatct gcatgtggaa tatgaacata ttctgcttga
     8641 gtaccttcaa taagatgtcc taaaatccac ccgccatctt cacaatgtga aggcaactgg
     8701 tgcttacagt aatagcaagt attacaggca gtgacacaag aaataattac tttatctccg
     8761 actttaaaat gattgacagc agaaccaact tcttcgacga taccaatgcc ttcatgacct
     8821 aaagttgttc ctggtttaca agcaggaaca tcgccaccaa ggatatgtag atcagtacca
     8881 caaatggttg ttttgacaag tcttacaacc gcatctgtgg gttttattac ttgaggttta
     8941 gccttatcag taagtgataa ttcattagct gaaagataag ttgcagctct catattagag
     9001 ttccttcaaa aattatttat tttcaaggct attataccac caaaagaaat cggttacaat
     9061 aattatttta aaataaaagc agtaattttc gtactgcttt aaattattat gcttttactt
     9121 tttttagaat aagaccaact aagacaccac aaattgcaaa actaatccat cccatagaat
     9181 attcacctaa aggaacaact ttagtaaaga atgtggtcaa agcggatgga atggtaaata
     9241 aaccagtcat ttcgccgagt tttgataagg catcgtataa agcagcgaca gcagtggcgg
     9301 caatagaagt ttggtagaca atgcagctac tttcaaattt ttgatcaaaa aagactaaga
     9361 aaattaagac tatagttaat ggatagagaa ggtagagaac tggtagtgac caacggataa
     9421 tctctgataa accaccaaaa tagaacgtaa tagcaattaa agtaaagatt gtagcccaag
     9481 taatgtgact aatctttgga agtaatttgt ggaagtattc cgcacaagca gtgattaatc
     9541 cagtagctgt tgttaagcaa gcaagaaaaa tagccgtacc taaaatagct tgtcccacaa
     9601 ttcctaaata gaagttagct gattgactaa gaacatgccc accgtctatt ggagtattgt
     9661 gaagtaagaa ggaaccattt gcaaatttaa acaagctttg tgaagttgca ccaatacgtc
     9721 ctacaaaaat ataaataaaa gctaaaagaa atgtagcgat agcaccagat ttaagtgcca
     9781 tagaagtaat ttcttttttg gtaattgctc cgtactgttt acttgcttga ataacgataa
     9841 tagcaaaagc aattgatgca agggcatcca ttgtaccata cccctgaatc agtcctgcta
     9901 ggaatggtaa atccttgaaa gcatcgctga cattcgttga agcgttatgt ggtgttccaa
     9961 cgatagcagc tggatggatg agtgaggcaa taattaaaat actaataaca gttagtaaaa
    10021 agggagttag atatttacca atacgatcta caatctgact aggtctaata gctaagaaat
    10081 aagaaatacc aaagaaaatt aaaccataga taattttgcc taaggagctg tcgcctaaga
    10141 taggttgaat accaactgag aatgaggttg cccctgttcg aggaatggca aagaaaggtc
    10201 caatagatag gtatagtgct actgcaaaga aaatagcata tgttttagag acaggtttgg
    10261 tgaagctttc aacatcggtt gctcctgagt acgccacagc tgcaacacca agtaggggta
    10321 aagttacgcc tgttaagcag aacccaatga tagcccacgc ccaattagaa ccagagtaga
    10381 tacctaagta ggctggataa ataagattac cggctccaaa gaacagcgca aaaagcatca
    10441 agccgataat gatataagtg ttttttagtt tcatcaatgt acctttctgt acaaaaaaat
    10501 attttatttt cgaaatt
//
LOCUS       DBJYSH010000071        10495 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.071, whole genome shotgun sequence.
ACCESSION   DBJYSH010000071 DBJYSH010000000
VERSION     DBJYSH010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 10495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10495
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.071"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            complement(161..889)
                     /gene="lrgB"
                     /locus_tag="ACUBV3_001401"
     CDS             complement(161..889)
                     /gene="lrgB"
                     /locus_tag="ACUBV3_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiholin-like protein LrgB"
                     /protein_id="HHU3847899.1"
                     /translation="MELLKTPIFGICFSLILYTIGQHLFKKSKGFFLLQPLFFAMVSG
                     IVILWLMSKGLGTDVKTFYTQAYKPGGDLIFWFLNPATIAFAVPLYKKNDVVKKYWVE
                     ILSSLVIGMIVSLMLIVAISKMVGLSQVGIASMLPQAATTAIALPITAAIGGNTAVTA
                     MACILNAVIIYALGKKLVSFFHLNDSKIGAGLGLGTSGHTVGAAFALELGELQGAMAA
                     IAVVVIGLVVDLVIPIFSHLIGLL"
     gene            complement(891..1346)
                     /locus_tag="ACUBV3_001402"
     CDS             complement(891..1346)
                     /locus_tag="ACUBV3_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HHU3847900.1"
                     /translation="MKHTSKETKTKEAPMFIQMSIYAAILLVSQMISMLLPKSLPIPT
                     TVIGLVLMYVLLTAKIIKVEWVDSFGALMISMIGFMFVPSGISVAANLDILKAEGLQL
                     VAVITISTVVMLVVVAYVARLILAIRELSVTDLFKKILPNHYSKKVEEK"
     gene            complement(1516..2256)
                     /locus_tag="ACUBV3_001403"
     CDS             complement(1516..2256)
                     /locus_tag="ACUBV3_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HHU3847901.1"
                     /translation="MKILILDDEMFARQELSFLVEHSQEVDNPEIFQAEDISEAEKIL
                     FRQQIDLIFLDISLSEENGFTLANQLSQLAHPPLVVFATAYDNYAVKAFESNAVDYIM
                     KPFEQQRVDMALSKVKKLSQLTTASDVEQAIPKKASVELLTLTLSDRSVVVKMQDIVA
                     ASVEDGELTVSTVQKTYTIRKTLNWFKSRAVAPYFLQIHRNTVINLEMIEEIQPWFNH
                     TLLLIMSNGEKFPVGRSYLKDLNEHLTM"
     gene            complement(2237..3982)
                     /locus_tag="ACUBV3_001404"
     CDS             complement(2237..3982)
                     /locus_tag="ACUBV3_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000930326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytS/YhcK type 5TM receptor domain-containing
                     protein"
                     /protein_id="HHU3847902.1"
                     /translation="MLMVLLFQRLGIIMILAFLLVNNSYFRQLIEERSKRETVVLVII
                     FGLFVIISNITGIEIKGDRSLVERPFLTTISHSDSLANTRTLVITTASLVGGPLVGSI
                     VGFIGGVHRFFQGSFSGSFYIVSSVLVGIVSGKIGDKLKENHLYPSTSQVILISIIAE
                     SIQMLFVGIFTGWELVKMIVIPMMILNSLGSTLFLAILKTYLSNESQLRAVQTRDVLE
                     LTRQTLPYLRQGLTPQSARSVCEIIKRHTNFDAVGLTDRSNVLAHIGVGHDHHIAGQP
                     VKTDLSKSVIFDGEPRIAQDKAAISCPDHNCQLNSAIVVPLKINDKTVGALKMYFAGD
                     KTMSEVEENLVLGLAQIFSGQLAMGITEEQNKLASMAEIKALQAQINPHFFFNAINTI
                     SALIRIDSDKARYALMQLSTFFRTSLQGGQDREVTLEQEKSHVDAYMNVEKLRFPDKY
                     QLSYDISAPEKMKLPPFGLQVLVENAVRHAFKERKTDNHILVQIKPDGHYYCVSVSDN
                     GQGISDTIIDKLGQETVAESKGTGTALVNLNNRLNLLYGSVSCLHFSSDKNGTKVWYR
                     IPNRIREDEHENFNS"
     gene            complement(4009..4653)
                     /locus_tag="ACUBV3_001405"
     CDS             complement(4009..4653)
                     /locus_tag="ACUBV3_001405"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HHU3847903.1"
                     /translation="MEKVIIFDMDGVIVDSEYTFLDNKTEMLREEGIDTDVSYQYQYM
                     GTTFEFMWQAMKEEFGLPKTVKEYIAEMNRRRQAIVARDGVRPIKGAQRLIHWLHQHG
                     YRLAVASSSPMVDIKRNLKELGVTECFEYMVTGEDVSSSKPAPDVFLRAAELLDVDPK
                     VCIVIEDTRNGSLAAKAAGMYCFGFANPDYPPQDLSMADKVISDYQDIYIYLPE"
     gene            complement(4776..5171)
                     /locus_tag="ACUBV3_001406"
     CDS             complement(4776..5171)
                     /locus_tag="ACUBV3_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HHU3847904.1"
                     /translation="MYNKVIMIGRLTAKPEMVKTPTDKSVTRATVAVNRRFKGSNGER
                     EADFINVVMWGRLAETLASYGTKGSLISVDGELRTRKYEKDGQTHYITEVLASSFQLL
                     ESRSQRAMRENNISGDLSDLVLEEEELPF"
     gene            5252..5968
                     /locus_tag="ACUBV3_001407"
     CDS             5252..5968
                     /locus_tag="ACUBV3_001407"
                     /EC_number="2.1.-.-"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HHU3847905.1"
                     /translation="MTNVTSYKEMLAKPWGKIQYEITFAQLSHIKNQNVLDFGAGFCL
                     TAQHLAKENNVTAIEPNPKLLYDNQSDNIYKILGSYEALRDLPNQSFDTIICHNVLEY
                     IDKHNHPAYFDEFSRLLKPNGELSLIKHNITGKILQSVIFSNDTSTAMELLTGEANFK
                     SASFDQGNIYTLEELKQNTNLLVERYQGIRTFYSLQPNHFKTETGWLNKMLAIELSVA
                     DKAPYKDIAFLQHITLKKSL"
     gene            complement(6022..6648)
                     /gene="ytpR"
                     /locus_tag="ACUBV3_001408"
     CDS             complement(6022..6648)
                     /gene="ytpR"
                     /locus_tag="ACUBV3_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtpR family tRNA-binding protein"
                     /protein_id="HHU3847906.1"
                     /translation="MIFTYNREHVGDTLMVIVKDSQGAKLDVDRRGQVARVYLQDSKE
                     TVAWNIFEVSSLIVIEGAGQITLSDQDIKILNAELLKEGFEDSLVNNIEPTFVVAQIK
                     EMIDHPDSDHLHICQVEINDGKTVQIVCGAPNASVGLKTVAALPGAMMPNGSLIFPGK
                     LRGEDSFGMLCSARELALPNAPQVRGIIELSDQVIVGESFDANKHWKN"
     gene            complement(6681..7004)
                     /locus_tag="ACUBV3_001409"
     CDS             complement(6681..7004)
                     /locus_tag="ACUBV3_001409"
                     /EC_number="1.8.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HHU3847907.1"
                     /translation="MILLESYEEIAAYIDSTKKVVFFFTADWCPDCQFIYPVMPSIEK
                     DFSDFVFVRVNRDDYIELAQQWNIFGIPSFVVVENGQELGRLVNKNRKTKAEITKFLA
                     EINHK"
     gene            complement(7001..7285)
                     /locus_tag="ACUBV3_001410"
     CDS             complement(7001..7285)
                     /locus_tag="ACUBV3_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4651 domain-containing protein"
                     /protein_id="HHU3847908.1"
                     /translation="MKNKKILFGTGLAGVGLLAAAGYTLTKKVTDYKRQQITQTLREF
                     FSQMGDIQVFYFNEFESDIKMTSGGLVLEDGRIFEFIYRQGVLDYVEVSK"
     gene            7446..7685
                     /locus_tag="ACUBV3_001411"
     CDS             7446..7685
                     /locus_tag="ACUBV3_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3847909.1"
                     /translation="MKEKLLVPALNCLLGLLFLGDHWFLFRLQNGELFAIVTLILLCT
                     TSFLINKNHPKNLTLISMIITFLAVLSFLSTFLLA"
     gene            7870..8937
                     /gene="pepA"
                     /locus_tag="ACUBV3_001412"
     CDS             7870..8937
                     /gene="pepA"
                     /locus_tag="ACUBV3_001412"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HHU3847910.1"
                     /translation="MSDLFNKIKTVTELDGIAGYEHNIRNFLRQEITPLVDQVETDGL
                     GGIFGVKNTHETNAPKVMVAAHMDEVGFMVSHIQPDGTFRVLEVGGWNPLVVSSQRFT
                     LYTRSGDAIPVISGSVPPHFLRGQSGGTTLPKISDIVFDGGFTDKNEAESFGIAPGDI
                     IVPKSETILTANQKHIMSKAWDNRYGVLMVTELLKSLKDQSLSNTLIAGANVQEEVGL
                     RGAHVSTTKFNPDIFLAVDCSPAGDIYGEQGKIGEGTLIRFYDPGHIMLKDMRDFLLT
                     TAEEAGIKYQYYAAKGGTDAGAAHLKNSGIPSTTIGVCARYIHSHQTLYAMDDFLQAQ
                     AYLQAIVNKLDRSTVDIIKGY"
     gene            9007..9777
                     /gene="proC"
                     /locus_tag="ACUBV3_001413"
     CDS             9007..9777
                     /gene="proC"
                     /locus_tag="ACUBV3_001413"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HHU3847911.1"
                     /translation="MKIGIIGVGKMASAIIQGLKQTQHDIVISGSCLERSKEIAERLD
                     VTYAESHQSLINQADIIMLGIKPQLFEKVLLPLDITKPIISMAAGISLARLSQLTRSD
                     LPLIRIMPNINAQILQSCTAICYNNHVSDELRQLTKEITDSFGSSFDIAETNFDTFTA
                     LAGSSPAYIYLFIEALAKAGVKYGFPKEQALSIVGQTVLASSQNLLQGQNSTSDLIDN
                     ICSPGGTTIAGLLDLEKNGLTHSVISAIDATIEKAKKL"
     gene            complement(9798..10463)
                     /locus_tag="ACUBV3_001414"
     CDS             complement(9798..10463)
                     /locus_tag="ACUBV3_001414"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HHU3847912.1"
                     /translation="MSFIIRKGKILALLIAFLVINQLVPILAVWLLKNHYQTPFTSIL
                     LIGLELLIIALFLYYAKVKQIIRWKALLTRKALVTIFLGWLSLRVPQIIGYLIMTMQG
                     VKDTANQTVIMELTKQLPLALMLIFTIIGAPIMEEIIFRYIIPKELFAKHQKWGFVIG
                     TLAFALIHSPSDIGSFIIYAGMGAILSFVYYKTEHLEYSIMVHFINNALAYSVLISTM
                     MNN"
BASE COUNT         3450 a         2095 c         1650 g         3300 t
ORIGIN      
        1 acttaactgg ttaattagaa aatttgttat tttatcatta ataacttaaa gttaggtaaa
       61 acacaattct agactctagc tcataaacca tgttaatcca atgtatttta atagacatca
      121 taaaaaggct gaataaattc agccttaacc acgctattag ttatagcaaa ccaatcaaat
      181 gactaaagat aggaataacc aaatcaacta ccaaaccgat aaccaccacc gctatagctg
      241 ccatcgcacc ttgcagttct cccaattcca atgcaaaggc tgctccgact gtatgaccag
      301 aggtccctag acctaatcct gcaccaatct tactatcatt caaatggaaa aatgacacta
      361 attttttacc taatgcataa ataataactg catttaagat acacgccatt gctgtcacag
      421 ctgtgttccc tccaattgct gctgttattg gaagagcaat tgctgttgtt gctgcttgtg
      481 gcaacattga agcaattccg acttgactaa gcccaaccat tttagaaata gcgacgataa
      541 gcataaggga aacaatcata ccgattacta aactgctgag aatttctacc caatattttt
      601 taacaacgtc atttttctta tagagaggaa ctgcaaaagc aattgttgct ggatttaaaa
      661 accaaaatat taaatcgcca cctggtttat aagcttgtgt ataaaatgtc ttaacatcgg
      721 ttcctaaacc ttttgacata agccaaagaa tgacaatacc actaaccatt gcaaagaaaa
      781 gaggctgcaa aaggaagaaa cctttactct tcttaaataa atgttgtcct atcgtataga
      841 gtattaaaga aaagcaaata ccaaagatgg gtgtttttaa gagttccata ttatttttcc
      901 tctactttct tactatagtg gttaggtaat atctttttaa ataaatctgt gacactgagt
      961 tctctgatag caagtattaa tcgtgcaacg tatgctacca ctactaacat aacaacagtt
     1021 gagattgtaa taacagcaac tagttgcaag ccttcagctt ttaggatatc aaggttcgct
     1081 gctactgata ttcctgaggg gacaaacata aaaccaatca tgcttatcat caaagcccca
     1141 aaagagtcaa cccactctac cttgataatt ttagcagtta gcaatacgta cattagcacc
     1201 aaaccaataa ccgtcgttgg gattggtagt gatttcggta ataacattga aatcatttgt
     1261 gaaactaata gaatagctgc atatattgac atttgaataa acattggagc ttcttttgtt
     1321 ttggtttctt tactggtgtg tttcatagtc actacccctt ccttttatta caatgtttta
     1381 acttgtttta ttgatagcta tattgtactt caaaataaat aaaacgtttt cattttcagc
     1441 ctcagttacc aaaaaaagaa gccgaattgc aaattcaagc ttctaaacaa caaagggaag
     1501 ttcgcaaaag cacttttaca tggttaaatg ctcatttagg tcttttaaat acgatcgacc
     1561 tactggaaat ttctctccat tagacataat tagtaacaaa gtatgattaa accaaggttg
     1621 aatttcctca atcatctcta gattaatcac tgtgtttcga tgaatctgga gaaaatatgg
     1681 tgctacagcg cgtgacttaa accaattgag tgttttccga atagtgtaag ttttctgaac
     1741 tgtgctaaca gttaattcgc catcttcaac actagctgca acgatatctt gcattttaac
     1801 aacaacactg cgatctgata atgtcaaggt taacaattca acactagctt ttttaggtat
     1861 tgcttgctct acatctgaag cagtggtgag ttggctcaat ttcttcactt ttgatagagc
     1921 catatcaacg cgctgttgtt caaacggttt catgatataa tcaacagcgt tactttcaaa
     1981 agctttcaca gcgtagttat cataagctgt cgcaaaaaca acaagtgggg gatgtgcaag
     2041 ttggctcaac tgattagcca aagtaaagcc attttcttca ctaagagaaa tatctaaaaa
     2101 aattaaatca atttgctgtc taaataagat tttttcagct tcactgatat cctcagcctg
     2161 aaaaatctca gggttgtcaa cctcctggct atgttcaact aaaaacgata attcttgtct
     2221 ggcaaacatt tcatcatcaa gaattaaaat tttcatgctc atcctccctt attctattag
     2281 gtattcgata ccaaactttt gtaccattct tgtcgctcga aaaatgaagg caacttacac
     2341 taccatataa taaattcagc ctgttattta gattaactag agcagtacct gtacccttac
     2401 tctctgcaac tgtttcttga cctaatttat caatgatagt atctgagatt ccttgtccat
     2461 tgtcactaac agaaacacaa taataatgac catctggctt tatttgaacc aatatatggt
     2521 tgtccgtctt acgttctttg aaagcatgtc taactgcatt ctctaccagt acctgtaaac
     2581 caaaaggcgg taacttcatt ttttctggtg cactaatatc ataagataac tgatatttat
     2641 cagggaaacg taatttttca acattcatat aagcatccac atgtgatttt tcttgctcaa
     2701 gcgttacctc acgatcctga ccaccttgca aacttgttct aaaaaaagta cttaactgca
     2761 tcagtgcata acgtgcttta tcagaatcaa tacggattaa tgcactaatt gtgttaatgg
     2821 cattaaagaa gaaatgaggg ttgatttgtg cttgtaaagc ctttatctct gccatactgg
     2881 ctaacttatt ttgttcctct gttatcccca ttgccagttg tcctgaaaat atttgcgcta
     2941 aaccaaggac taggttttcc tccacctcag acattgtctt atctcctgca aagtacattt
     3001 ttaaggcacc cacagtttta tcatttattt ttagaggaac tacaatagca gaatttaact
     3061 gacagttgtg atctggacaa gaaatcgccg ctttatcttg cgcaattctt ggttcgccat
     3121 caaaaataac acttttagat aggtctgttt tgactggttg tcctgcaata tggtgatcat
     3181 ggccaacacc aatatgagct aatacgtttg accgatctgt taatcctaca gcatcaaagt
     3241 tagtatgcct ctttataatt tcgcaaacgc tcctagcaga ttgcggtgtc aaaccttgtc
     3301 tgaggtaggg cagagtctgt cgagtcaatt caagaacatc tctcgtttga actgcgcgta
     3361 actgactttc atttgacaaa taagttttca aaatcgcaag gaaaagtgtg gaacctaaac
     3421 tatttaaaat catcattgga atgacaatca ttttgacaag ttcccatcct gtaaaaatac
     3481 caacaaatag catctggata ctttcggcaa taatactaat taaaataact tggcttgttg
     3541 aagggtagag atggttttcc ttaagcttat caccaatctt tccgctaaca atgccgacta
     3601 gaactgaact gacaatatag aaagaacctg aaaagcttcc ttgaaaaaag cgatgaactc
     3661 ctccaataaa accaacaatt gatccaacca gaggtccacc aaccaaactt gccgttgtaa
     3721 taactaaagt ccttgtatta gcaagtgagt cagaatggga aatcgttgtt agaaaagggc
     3781 gctcgaccaa acttcgatcc ccttttattt caattcctgt tatattagat ataataacaa
     3841 acaagccgaa aatgatgaca agtactaccg tttcacgttt agaccgctct tcaattaact
     3901 gtctgaaata actattattt accaataaaa aggctaaaat cataataatt cctagccttt
     3961 ggaataacaa caccatcaac atatgcttat cctcctcaag ataccatttt actccggaag
     4021 ataaatatag atatcttgat aatctgaaat aaccttgtct gccattgata aatcttgcgg
     4081 tggataatca ggattagcaa agccaaagca atacattcct gctgccttag cagctaaact
     4141 cccatttctt gtatcttcta taactataca aactttaggg tctacatcta aaagctctgc
     4201 agctctaaga aaaacatctg gtgctggttt agaagacgat acatcttctc ctgtaaccat
     4261 gtattcaaag cattctgtca cacccaactc ttttagatta cgcttaatat ctaccattgg
     4321 agatgaggac gcaactgcta agcgatagcc atgttggtgt aaccaatgaa tcagtcgctg
     4381 agctcctttg atgggcctaa caccatcacg cgccacaatc gcttgtcgac gtctgttcat
     4441 ctcagcaata tactctttta ctgttttcgg taagccaaac tcctctttca tagcttgcca
     4501 catgaattca aaagtagtcc ccatgtactg gtattgatac gagacgtccg tatcaatacc
     4561 ctcctcacgt aacatttctg tcttattatc taaaaacgtg tactcggaat caacaataac
     4621 gccgtccata tcaaaaataa tgactttttc catcttaaac ctgctttcta tcccctttat
     4681 tgtaatcgtt tgcctttcta ttttcaataa aaaaaggttg ctcccacaga aagcaaccag
     4741 tatatttaga aatgatattt tgagagtgat tttatttaaa aggggagctc ctcttcttcc
     4801 aatactaaat ctgacaaatc accagaaatg ttattttcac gcatagcacg ttgggaacgg
     4861 ctttctagca actgaaatga tgatgctaat acttcagtga tatagtgcgt ttgaccatcc
     4921 ttttcgtact tgcgcgtacg caattcacca tctactgaaa ttaaagagcc ctttgtccca
     4981 tagctcgcaa gggtttccgc tagacgaccc cacataacca cattaataaa atctgcttca
     5041 cgctcaccat tacttccttt aaagcgtcta ttaacagcaa cagttgcacg cgttactgac
     5101 ttgtcagttg gtgtttttac catctcaggc tttgctgtta gacgcccaat cataataact
     5161 ttattataca ttgactacct cctcactatt attattcgaa aataaggaag aaaaatgcta
     5221 aaatatatga aattgttctg ggagaattaa gatgacaaat gtaactagtt ataaagaaat
     5281 gctggctaaa ccttggggaa aaatacaata tgaaataact tttgctcaat taagccatat
     5341 caaaaatcag aacgttttag attttggagc tggtttttgt ctcaccgcac aacatctagc
     5401 aaaagaaaac aatgtaactg ctattgaacc caatcctaaa ctgctttacg ataatcaaag
     5461 tgacaatatt tataaaatcc taggttccta cgaagcatta agagatttgc ctaatcaaag
     5521 ttttgatact attatttgtc ataatgttct tgaatatata gacaaacata atcatcctgc
     5581 ttattttgac gaattttctc gacttctaaa acctaatgga gaactatctc tcatcaaaca
     5641 taatataacc ggtaaaatat tacaatcagt tatttttagc aatgatacct ccactgcgat
     5701 ggaactctta acaggagaag caaactttaa aagtgccagt tttgaccagg gaaatattta
     5761 taccttagaa gaactaaaac aaaatacaaa tttattagtt gaacgttatc agggtattcg
     5821 taccttctat tcattacaac caaaccattt taaaactgaa actggttggc taaacaaaat
     5881 gcttgctatt gagctaagtg ttgctgacaa agctccttat aaagatattg cttttttgca
     5941 acacatcaca cttaaaaagt cattgtaact tcttaagttt acaactaaaa atcaacttag
     6001 ctgaagctaa gttgattttt actaattttt ccaatgttta ttggcgtcga aagactctcc
     6061 aacaattacc tgatcagata attcaatgat acctctgact tgaggagcat ttggaagagc
     6121 taactcgcga gcactacata acattccaaa gctatcttct ccacgaagtt ttcctgggaa
     6181 aataaggcta ccatttggca tcatagctcc tggaagagct gcaactgttt taagacctac
     6241 tgaagcatta ggtgccccac aaacgatttg aaccgtcttc ccatcgttga tttctacttg
     6301 acagatgtgt aaatggtcac tatctggatg atcaatcatt tctttaattt gtgccacaac
     6361 aaatgtaggt tcaatattat taacaagaga gtcttcgaat ccttctttca gtagttctgc
     6421 attaaggatt ttaatatctt gatcagataa agttatttga cctgctccct caataacaat
     6481 taaacttgac acttcaaaga tattccaagc aactgtttct ttactatctt gcagatagac
     6541 gcgtgctact tgtccgcgac gatcaacatc tagcttagct ccttgactat ccttaacaat
     6601 aaccattaag gtatcaccaa catgttctct attataagta aaaatcattt attctccttt
     6661 aaattttgct attttctgtc ttatttatgg ttaatttcag cgagaaattt agtaatttca
     6721 gcctttgttt tgcgattctt attaaccaaa cgacccagtt cctgaccatt ttccacaaca
     6781 acgaaactcg gaatcccaaa aatattccac tgctgagcta attctatata gtcatcacga
     6841 ttaacacgta caaagacaaa atctgagaaa tctttttcaa ttgatggcat tactggatat
     6901 ataaattgac aatctggaca ccaatctgct gtaaaaaaga aaacaacttt cttagtgcta
     6961 tcaatatagg ctgctatttc ttcataagat tctagtaaaa tcatttactc acctccacat
     7021 aatcaagaac accttgacga taaatgaatt cgaaaattct gccatcttcc aagacaagac
     7081 caccactggt cattttaata tcagattcaa attcattaaa ataaaatacc tgaatatcac
     7141 ccatctgact aaaaaattct cttaaggtct gagtgatttg ctgacgttta taatctgtta
     7201 cttttttagt tagggtataa ccagcagctg ccagtaaacc cacaccagca aggccagtcc
     7261 caaataagat ttttttattt ttcataactg ttattatacc ataccccccg gaattagaca
     7321 caggaattga tggaagaaat ccacataaca gttatgaaaa aaaacaactc attccttgtg
     7381 gtataatagg atgacattgt cttaagatac cattatggtt aatcattcaa acagaaagaa
     7441 aaaacatgaa agaaaaatta ttagtacctg ctttaaattg tttgctaggt ctactcttcc
     7501 taggtgatca ctggttttta tttcgtcttc aaaatggaga actttttgct attgtgacgc
     7561 taatactgct ttgcacaact tcatttttaa tcaataagaa tcaccccaaa aatttgactt
     7621 tgatttctat gattatcact tttctagccg tattatcttt cctatctacc tttttactag
     7681 cttaagccga taaacattat ctccgttgta ttcaacccag ttttcactat aacaggcttt
     7741 gtcaatcata acagtcttct ggtagtactt gatattcttt ttgaaagtct ttatattgac
     7801 tgaaacctgt tctgttcttt atttttaatt aaaaggtgct aaaatagtat ttgtagaatt
     7861 gaggtaatta tgtcagattt atttaacaaa attaaaaccg taactgagct tgatgggatt
     7921 gctggctatg aacacaatat ccgcaacttc cttcgtcaag aaataactcc tttagttgat
     7981 caagttgaga cagacggact tggtggaatt tttggagtta aaaatactca tgagactaat
     8041 gctcctaaag tcatggttgc tgcccatatg gatgaagtcg gctttatggt tagtcatatt
     8101 cagccagatg gaacatttcg tgtacttgag gttggaggat ggaatcccct agtagtcagc
     8161 tcacaacgct ttaccctcta cacacgttct ggtgatgcta ttcctgttat atcaggctca
     8221 gttcctcctc actttcttcg tggacaaagc ggtggaacga cattacccaa aattagtgac
     8281 attgtttttg atggaggatt cacagataaa aatgaagctg aaagctttgg cattgctcct
     8341 ggcgatatca ttgttcctaa atctgaaacc attttaactg caaatcaaaa acatattatg
     8401 tcaaaagctt gggataatcg ctatggtgtg cttatggtga ccgaattgct aaaaagctta
     8461 aaagatcaaa gtcttagcaa cacacttatt gctggggcaa atgttcaaga agaagtcgga
     8521 cttcgtggcg cacatgtttc aacaactaaa ttcaacccag atatcttctt agctgtcgat
     8581 tgttccccag ctggagatat ttatggggaa caaggcaaaa taggagaggg aaccttaatc
     8641 cgtttttatg atcccggaca tatcatgctt aaagatatga gagatttctt gcttacaaca
     8701 gctgaagaag caggtataaa ataccaatat tatgctgcaa aaggtggtac cgatgctggg
     8761 gctgctcacc taaaaaatag tggtattcct tctacaacta tcggtgtctg tgcacgctac
     8821 attcattctc atcaaacact ctacgctatg gatgattttc tacaagcaca agcttacctt
     8881 caggctatcg ttaacaaatt agaccgctcg acggtggata ttattaaagg ttattaagat
     8941 agaaaggatg ggagtttcat aaggtagaca cattttagta tctcctttca tccccatatc
     9001 aacattatga aaattggaat tatcggcgtt ggaaaaatgg ccagtgcaat tattcaaggc
     9061 ctcaaacaaa cccaacatga cattgtcata tccggatctt gcttagagcg ttcaaaagaa
     9121 attgcagaaa gacttgatgt tacttacgcc gaatctcatc agtcattaat caatcaagct
     9181 gatattatca tgttaggtat taaacctcaa ctatttgaaa aggttcttct tccacttgat
     9241 atcacaaaac caatcatctc aatggcagca ggaatctcct tagcgcgttt aagccaatta
     9301 actcgttctg atttaccatt gattcgtatt atgccaaata ttaacgcaca aatacttcaa
     9361 agttgcacag ctatttgtta caacaatcat gtatcggatg agttacgaca attaaccaaa
     9421 gaaattacgg atagttttgg aagtagcttt gatatagcag aaaccaattt tgataccttc
     9481 actgctctag caggttctag ccctgcctat atctatctat ttatagaagc cttagctaaa
     9541 gcaggtgtaa aatatggatt tccaaaagaa caagcactct ctattgtagg acagactgtc
     9601 ttagcttcta gtcaaaacct attacaagga caaaatagca cctctgactt aatcgacaac
     9661 atttgtagcc caggcggaac tactattgct ggccttttag acttagaaaa aaatggtctg
     9721 acgcatagtg ttatttctgc cattgatgct actattgaga aagcaaaaaa actttgagca
     9781 aattgctcaa agttttttta gttattcatc attgtactga ttaacacgct ataagcaaga
     9841 gcattgttaa taaaatgaac cattatacta tattctaagt gctcagtttt ataataaaca
     9901 aaagataaaa tagcacccat cccagcataa ataataaaac tacctatatc agaaggggaa
     9961 tgaataagag caaaagctaa agtaccgatg acaaaacccc atttttgatg tttcgcaaaa
    10021 agttctttag ggataatata gcgaaatata atttcttcca taataggggc accaattatg
    10081 gtaaaaatta acatcaaagc tagtggaagt tgctttgtta attccataat tactgtttga
    10141 tttgctgtat ctttaacccc ttgcatagtc ataatgaggt aaccaataat ttgtggaacc
    10201 ctcaatgata accaacctaa gaaaatagta actaatgctt tccttgtcaa taatgctttc
    10261 catctaatta tttgtttaac cttggcataa taaaggaaaa gagcaataat aagtaattct
    10321 aagccaatta acaatatact agtaaaaggc gtttgatagt gattctttag gagccacaca
    10381 gccaatatag gaacaagttg attaataact aaaaatgcta ttagcaaagc taaaattttg
    10441 ccttttctaa taataaatga cacaaaaacc tccaaaataa attattaact ttttt
//
LOCUS       DBJYSH010000072        10139 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.072, whole genome shotgun sequence.
ACCESSION   DBJYSH010000072 DBJYSH010000000
VERSION     DBJYSH010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 10139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10139
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.072"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            complement(<1..929)
                     /locus_tag="ACUBV3_001415"
     CDS             complement(<1..929)
                     /locus_tag="ACUBV3_001415"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HHU3847913.1"
                     /translation="MIRQFLREHLIWYILYIMMFVLFFISFYLYHLPMPYLFNSLGLN
                     VIVLLGISIWQYSRYRKKMLHLKYFNSSQDPSFELQPSDYAYFNIITQLEAREAQKVS
                     ETIEQTNHVALMIKMWSHQMKVPLAAISLMAQTNHLDPKEVEQQLLKLQHYLETLLAF
                     LKFRQYRDDFRFEAVSLREVVVEIIKSYKVICLSKSLSIIIEGDNIWKTDKKWLTFAL
                     SQVLDNAIKYSNPESKIIISIGEESIRIQDYGIGILEEDIPRLFEDGFTGYNGHEHQK
                     ATGMGLYMTKEVLSSLNLSISVDSKINYGTAVS"
     gene            complement(926..1594)
                     /locus_tag="ACUBV3_001416"
     CDS             complement(926..1594)
                     /locus_tag="ACUBV3_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HHU3847914.1"
                     /translation="MSQEQGKIYIVEDDMTIVSLLKDHLSASYHVSSVSNFRDVKQEI
                     IAFQPDLILMDITLPYFNGFYWTAELRKFLTIPIIFISSSNDEMDMVMALNMGGDDFI
                     SKPFSLAVLDAKLTAILRRSQQFIQQELTFGGFTLTREGLLSSQDKEVILSPTENKIL
                     SILLMHPKQVVSKESLLEKLWENDSFIDQNTLNVNMTRLRKKIVPIGFDYIHTVRGVG
                     YLLQ"
     gene            complement(1704..3659)
                     /locus_tag="ACUBV3_001417"
     CDS             complement(1704..3659)
                     /locus_tag="ACUBV3_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HHU3847915.1"
                     /translation="MFYLKIAWHNLKHSIDQYIPFLLASLLLYSLTCSTLLILMSAVG
                     RDMGTAATVLFLGVIVLSIFAVVMEHYSYNILMKQRSSEFGLYNILGMNKRQVARVAS
                     LELFIIYIFLISIGSLFSAFFAKFIYLIFVNIINYHALNLSLSLWPFIICIVIFTGIF
                     LTLEVPVIRHVHLSSPLSLFRKKQQGEKEPKGNLILAILALVAIAIAYTMALTSGKAP
                     ALAVIYRFFFAVLLVIAGTYLFYISFMTWYLKRLRQNKHYYYKSEHFVSTSQMIFRMK
                     QNAVGLASITLLAVMALVTIATTVSLYSNTQNVVTGLFPESVSLSIDNSKGDAKNIFE
                     EKILKKLGKSSKEAITYNQTMISMPVSQSSELNITSKNVKHVDITKTGFMYLITQNDF
                     RRLGHQLPKLKDNQVAYFVQKGDSRLKKINLLGNKFDVVKNLKEAYVPETTNTYNPGL
                     IIFANNKQIDNIRKAYLPYTKNINTFPKTFKAYLDLNSQEINSISKNDIIEVDGKYVG
                     NISTKQSFLKEGYQMFGGLLFTGFLLGISFLLGIALIVYYKQYSEGHEDKRSYRILQE
                     VGMSKKLVKRTINSQIMIFFFQPLVVAVIHFGVAIPMLKQMLLVFGVLNSTIVYVVSG
                     LTVLAISIIYFIIYRITSRTYYHIIER"
     gene            complement(3661..4413)
                     /locus_tag="ACUBV3_001418"
     CDS             complement(3661..4413)
                     /locus_tag="ACUBV3_001418"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HHU3847916.1"
                     /translation="MLLEINHLEKVFRTRFSKEETRALQDVDFKVEQGEFIAIMGESG
                     SGKTTLLNILATLEKPTNGQVILNGEDITKIKEAKLASFRLKNLGFVFQDFNLLDTLS
                     VRDNIYLPLVLDRKRYKEMDHRLSELSSHLRIDDLLDKRPFELSGGQKQRVAIARSLI
                     TNPQILLADEPTAALDYRNSEDLLNLFETINLDGQTILMVTHSANAASHAKRVLFIKD
                     GRIFHQLYRGNKNNSEFNKDISLTMSAILGGE"
     gene            complement(4439..5401)
                     /locus_tag="ACUBV3_001419"
     CDS             complement(4439..5401)
                     /locus_tag="ACUBV3_001419"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001253131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HHU3847917.1"
                     /translation="MRRKIVLLFVVPMLIVLGILGVVVHYYGSALNIYLLPPSSERYG
                     RVILDRVEQRGLYSQGRQWQIIRQRSEKKLKTSKSYQESRNIVQEAVRYGGGKHSQIL
                     SKETVRRDTLDSRYPEYRRLNEDILLITIPSISKLDKRSISHYSGKLQNILMEKSYKG
                     LILDLSNNTGGNMIPMIGGLASILPNDTLFHYTDKYGNKKTITMKNIPLEALKISRKT
                     INTKHVPIAIITNHKTASSAEMTFLSFKGLPNVKSFGQATAGYTTVNETFMLYDGARL
                     ALTTGIVSDRQGYKYENTPILPDQVTSLPLQESQSWLKSRINQN"
     gene            5567..6055
                     /locus_tag="ACUBV3_001420"
     CDS             5567..6055
                     /locus_tag="ACUBV3_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HHU3847918.1"
                     /translation="MAKTSKKLTKSLGPIGKLISIIPDTTELIGKAIDNSRPIIEKEL
                     DRRHEKKQTYTQLDNVVHLDINDAQVYLEQRHFNVSRIVAEPNSKWATLHPNQVVNMS
                     PKAGRVRIGSLVKLYYITEDILEESKTLLLSKSEKSKARQQRLTEVLSTPSQLLKKNR
                     NK"
     gene            complement(6087..6935)
                     /locus_tag="ACUBV3_001421"
     CDS             complement(6087..6935)
                     /locus_tag="ACUBV3_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HHU3847919.1"
                     /translation="MKIKKLLGLTTTVVISALILGACGQSKNEDAKVVRVGTMVKSKT
                     EKARWDKIEELVKKKGVKLKFTEFTDYTQPNKALESDEIDINAFQHYNYLNNWNKANK
                     TNLVSVAETYFTSFRLYSGTKNGKGKYQTVSEIPNKATITIPNDAVNESRSLYLLQSA
                     GLLKLKVSGDTLATMSDVVSNPKSLDLKEVDAAQTARSLDSTDAAVINNDFVTEAGIN
                     PKSAIFIEPKSKNAKQWYNLLVAQKGWQDKSKAKAIKEVVKAYHTDAVKKVIEKTSQG
                     LDQPVW"
     gene            complement(7068..7811)
                     /locus_tag="ACUBV3_001422"
     CDS             complement(7068..7811)
                     /locus_tag="ACUBV3_001422"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000559006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HHU3847920.1"
                     /translation="MHYKELLESSEKAYLETNRYKHFKNIITSERYFSNYISYKIMPT
                     VSEFLQDMIYLADEQKDYQSDFAFVFFEENAELSSELQRTLSQQGFESEKHIIFTNSL
                     NHLKLFEKEFDDITFTKLNENYLEKYLKFKYKQSIVYGEIYAKQMYNDNRVNLLNNNA
                     KVYLAIKDNQIIGDVTAWDYGEYVEIDDFYVQESYRGQGIGTRLQFDASRGFKKVILI
                     SEEENRKMYEHQGYEEVAYYWTALRSPRK"
     gene            complement(7889..9223)
                     /gene="trmFO"
                     /locus_tag="ACUBV3_001423"
     CDS             complement(7889..9223)
                     /gene="trmFO"
                     /locus_tag="ACUBV3_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HHU3847921.1"
                     /translation="MSQSYINVIGAGLAGSEAAYQIAKRGIPVKLYEMRGVKSTPQHK
                     TDNFAELVCSNSFRGDSLTNAVGLLKEEMRRLDSIIMRNGEAHRVPAGGAMAVDREGY
                     SEAVTEEIHKHPLIEVIRDEITDIPGDAITVIATGPLTSDSLAAKIHELNGGDGFYFY
                     DAAAPIVDKNTIDMNKVYLKSRYDKGEAAYLNCPMTKEEFMAFHEALTTAEEAPLNSF
                     EKEKYFEGCMPIEVMAKRGIKTMLYGPMKPVGLEYPEDYKGPRDGEFKTPYAVVQLRQ
                     DNAAGSLYNIVGFQTHLKWGEQKRVFQMIPGLENAEFVRYGVMHRNSYMDSPNLLNQT
                     FATRKNPNLFFAGQMTGVEGYVESAASGLVAGINAVRRFNGESEVVFPQTTAIGALPH
                     YITHTDSKHFQPMNVNFGIIKELEGPRIRDKKERYEAIATRALKDLEKFLNY"
     gene            complement(9337..10035)
                     /locus_tag="ACUBV3_001424"
     CDS             complement(9337..10035)
                     /locus_tag="ACUBV3_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000936162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HHU3847922.1"
                     /translation="MLPAYIKIHDAIKKEIDKGTWKIGQRLPSERDLADDYSVSRMTL
                     RQSITLLVEEGILERRVGSGTYVASHRVQEKMRGTTSFTEIVNSQGRKPSSKLISFQR
                     KLANETEIQKLNLSQSDYVVRMERVRYADKVPLVYEVASIPENLIKGFEQSEVTEHFF
                     KTLTENGYEIGKSQQTIYARNASERVASHLEVNAGHAILALTQVSYFTDGKPFEYVHG
                     QYVGDRFEFYLENN"
BASE COUNT         3284 a         1857 c         1520 g         3478 t
ORIGIN      
        1 atagaaacag cagtcccata attaattttg ctatccaccg aaatggacaa attcagacta
       61 gataagactt cttttgtcat atataacccc atgcctgttg ccttttggtg ctcatgaccg
      121 ttgtaacccg taaagccatc ttcaaaaagt ctagggatat cctcttcgag tatgccgata
      181 ccgtagtctt gtattctaat actctcttct cctatgctta ttattatctt tgactcagga
      241 ttagaatatt ttatggcatt atctagcacc tgtgaaagag caaaagttaa ccactttttg
      301 tctgttttcc agatattatc gccttcaatt atgatagata agcttttgga tagacaaata
      361 accttatacg attttataat ttctactact acttctctaa ggctaacagc ttcaaaacga
      421 aaatcgtcac gatattgtct aaatttcaaa aatgctaaca acgtttcaag ataatgttgc
      481 aatttcaata attgttgttc aacttcctta ggatcgagat gatttgtctg ggccattaat
      541 gaaatagctg ccaatggaac tttcatttgg tgcgaccaca tctttatcat aagtgcaaca
      601 tgattggttt gttcaattgt ttcagaaact ttttgcgctt ctctagcttc taattgtgta
      661 ataatattaa aataagcgta atcactcggt tgaagttcga aagaggggtc ctgactacta
      721 ttaaaatatt tgagatgtaa catttttttc ctgtaacgac tgtattgcca aatactaatt
      781 cctagtaaaa caataacatt taaacctaag gaattaaaca aatagggcat tggtaaatga
      841 tatagataga aactaataaa aaataggaca aacatcatga tatataaaat ataccaaatc
      901 aagtgttctc ttaaaaactg gcgtatcatt gtagtaaata cccaactcct ctcactgtat
      961 gaatgtaatc aaaacctatt gggacaattt ttttacgtaa gcgtgtcata ttaacattaa
     1021 gtgtattttg atcaataaaa ctatcatttt cccaaagttt ctctaataga gactcttttg
     1081 agactacttg tttaggatgc atgagcaaga tagataggat tttattttct gttggcgata
     1141 aaataacctc tttatcttgg ctagacaata acccttctct tgtcaacgta aatcccccaa
     1201 aagttaattc ctgttggata aattgttgac ttctccttaa aatagcagtt agcttagcat
     1261 ctaatacagc tagagagaat ggttttgaaa taaagtcatc accccccata tttaatgcca
     1321 taaccatatc catttcatca ttactagatg aaatgaaaat aataggaatt gttaaaaact
     1381 tacgcaattc tgcagtccag taaaaaccat taaaataggg taacgtaata tccattagta
     1441 tcaaatcggg ttgaaatgcg ataatttctt gtttcacatc acgaaaattg ctgacactag
     1501 agacatgata gctagctgat aaatgatctt ttaaaagtga cacaatcgtc atatcatctt
     1561 ctacaatata aatttttcct tgctcttgtg acatatcccc tcctaaaata gtacgaaatc
     1621 tctttctttc atttagtata acaaattttt tagtctatcg gtttctatta atgcaaaagg
     1681 attggtaaaa taaccaatcc tttttagcgt tcaataatat gataataagt tcgactagtt
     1741 atacggtaaa tgataaagta aattatagaa atcgcaagta ctgtcaaacc acttactaca
     1801 tatacgattg ttgagttaag caccccaaat accaaaagca tctgttttag cattggaata
     1861 gcaactccaa agtgaataac tgctaccact aaaggttgga agaagaaaat cataatttga
     1921 gaattaatag tccgtttaac taattttttg ctcataccta cttcttgtaa gatacgataa
     1981 gatctcttat cttcatgacc ttctgagtat tgcttatagt aaacaattag agctatacct
     2041 agcaaaaagc ttatacctaa cagaaatcca gtaaataata acccaccaaa catttgataa
     2101 ccttctttta gaaaactctg tttagtgcta atatttccaa catacttacc atctacctct
     2161 attatgtcgt ttttagaaat tgaatttatc tcttgactat tcaaatccaa atatgcctta
     2221 aaggtttttg gaaaagtatt gatgttctta gtataaggta aatatgcttt tctaatattg
     2281 tcaatttgtt tgttgtttgc aaatataatc aaacccgggt tgtaagtatt tgtagtctct
     2341 gggacataag cctcttttaa attttttaca acgtcaaatt tatttcccaa tagatttatt
     2401 tttttcaaac ggctatcacc tttttgaaca aagtaagcca cttggttatc ttttaatttt
     2461 ggtaattggt gacctaaacg acggaaatca ttctgagtaa ttaggtacat aaaacccgtt
     2521 tttgtgatat caacatgttt gacgttttta gatgtgatat taagttcact tgattgacta
     2581 actggcatcg aaatcattgt ctgattataa gtgatagctt ccttagatga cttacctagt
     2641 ttcttcaaaa tcttttcttc aaatatattt ttcgcgtcac cttttgaatt atctattgat
     2701 aaacttactg attctggaaa tagtccggta acaacatttt gtgtatttga atagagtgag
     2761 actgttgtag caatagtaac tagagccata acagctaata aagtgatact tgctaaccct
     2821 actgcatttt gcttcattcg aaaaatcatt tgcgaagttg atacaaaatg ctcagattta
     2881 taataataat gcttgttttg acgcaacctt tttaagtacc atgtcataaa actaatataa
     2941 aaaagataag taccagcaat aactaaaagt actgcaaaga agaaacgata gataacagct
     3001 aatgcaggtg ctttacctga agtaagagcc attgtataag cgatggcgat agctactaac
     3061 gctaaaattg caagtataag attacctttt ggttcttttt ctccctgttg tttctttcta
     3121 aaaagactta atggggatga taaatgaaca tgtcgaataa ctggaacttc taaagtcaga
     3181 aaaatacctg taaatataac gatacaaata ataaatggcc ataaacttaa actaagattt
     3241 agtgcatgat agttaataat gttgacaaaa attaaataaa taaatttagc aaaaaaagca
     3301 ctaaacagac ttcctataga aataagaaat atataaataa taaacagctc tagactagct
     3361 acacgcgcaa cttgacgttt attcatcccc aaaatgttat acagtccaaa ttcactacta
     3421 cgctgtttca tcaagatatt gtagctataa tgttccatga ctaccgcaaa gattgacaaa
     3481 acaatcactc caagaaaaag aaccgttgcc gctgtcccca tatctcttcc aacagcactc
     3541 attaagatta gtagcgtaga acaagtcaat gaataaagta ataaactggc taagaggaat
     3601 ggtatgtact ggtctataga atgttttaaa ttatgccagg ctattttgag ataaaacata
     3661 ttattcaccc cctaatatgg cagacattgt taaagaaata tctttattga attcgctatt
     3721 attcttatta ccacgataaa gttggtgaaa aatacgtcca tctttgataa ataaaacgcg
     3781 tttggcatga ctagcagcat ttgctgaatg ggttaccatc aaaatagttt gtccatccaa
     3841 gttaatagtt tcaaataaat tcagcaagtc ttctgaatta cggtaatcta acgctgctgt
     3901 tggttcatct gctaataaaa tttgagggtt ggttatcaaa ctacgagcaa tcgctacacg
     3961 ttgcttttgc ccaccagaga gctcgaaagg tctcttatct aataagtcat caattctcag
     4021 atgagaagat aattctgaca aacgatgatc catttctttg taacgtttac gatcaagaac
     4081 taaaggaaga tagatattat ctcttacaga gagagtatcc aaaaggttga agtcctgaaa
     4141 gacgaaacca agatttttca aacgaaaact cgctaatttt gcctctttaa ttttcgtaat
     4201 atcttcccca tttaaaatca cttgtccatt tgtcggtttt tctaaagtag ctaaaatatt
     4261 tagcagagtt gtttttcccg aaccagactc tcccataata gcaatgaatt ccccttgttc
     4321 aactttaaaa tcgacgtctt gtagagccct tgtctcttct tttgaaaaac gtgtacgaaa
     4381 aactttttct aagtgattga tttctaataa catataattc tcctttattt attataccct
     4441 aattttgatt gattctactc tttaaccaag actgtgactc ttgtaaagga aggctggtta
     4501 cttgatctgg taagataggg gtgttctcgt atttatatcc ttgtcgatca ctaactattc
     4561 ctgtagttaa agctaatcta gcaccgtcgt aaagcatgaa agtttcatta acagtcgtat
     4621 atcctgctgt agcttgacca aagcttttaa cattaggtaa tcctttaaat gataaaaaag
     4681 tcatttctgc cgaactagca gttttatgat tagttataat agcgattgga acatgcttag
     4741 tattgattgt tttcctagat atttttaatg cttctagcgg tatatttttc atcgtgattg
     4801 ttttcttatt tccatactta tcagtataat gaaatagggt atcatttggt aaaatagagg
     4861 ctaacccccc aatcataggg atcatattgc cgccagtatt attcgataag tctaaaatga
     4921 gtcccttata acttttttcc atcaatatat tttgtaattt accactataa tggctaatag
     4981 atcgtttatc taacttactt atagaaggaa tagtaattaa gagaatgtct tcgtttaatc
     5041 ttcgatattc tggatatcga ctatctaggg tatctcttcg tacagtctct ttggataata
     5101 tttgactatg tttaccgcct ccataccgta ctgcttcctg tacaatgttg cgactctctt
     5161 ggtaagattt tgacgttttc aacttttttt ctgatcgttg tcttataatt tgccattgac
     5221 gaccttgcga atacaaacca cgctgttcaa cacgatctaa aataacacgt ccataacgtt
     5281 cgctactagg aggtaataga taaatattta atgctgaacc ataataatga actaccactc
     5341 ctaaaattcc taaaactata agcattggaa ctacaaaaag taaaactatt tttcgccgca
     5401 tctcttgctc cccctttctc tttaagtcta ttatatggca aaatggttta ctataacctt
     5461 acatcttaag acctatttct tacaaatttg aaagttttaa aagctttata aactataatt
     5521 ttgatataat atcttacata acaaatggaa agtacggtga aaacatatgg ccaagacaag
     5581 taaaaaattg acaaaatctt tagggcctat tggtaaatta atttcaataa tccctgacac
     5641 aactgaactt attggaaaag caattgataa ttctcgtcca attattgaaa aagaacttga
     5701 ccgtcgtcat gaaaaaaaac agacttatac tcagcttgat aatgtagtac accttgatat
     5761 caatgatgcg caagtttact tagagcaacg ccattttaat gtatcaagga ttgttgctga
     5821 accaaattca aagtgggcaa ccctacatcc taatcaagtt gtgaatatgt cgcctaaagc
     5881 tggtagggtc agaatcggtt cattagtgaa gttgtactat attacagagg atatccttga
     5941 agaaagtaag acactacttt tatctaaatc cgaaaaaagt aaagcacgac aacaacggtt
     6001 aacagaagta ttgagtaccc ctagtcaatt actcaaaaaa aatcgaaata aataagcatg
     6061 atcgaattga ccatgcttat ttatttttac caaacaggtt gatctaaacc ttgagatgtt
     6121 ttttcgataa cttttttaac cgcatctgta tggtaagctt tcacaacttc tttaatggct
     6181 ttagcttttg atttgtcttg ccaacctttt tgagcaacta aaaggttgta ccattgtttt
     6241 gcatttttac tttttggttc aataaaaata gctgatttag gatttattcc tgcttctgtc
     6301 acaaaatcat tattaataac tgctgcatct gtggaatcca aagaacgtgc ggtctgtgct
     6361 gcatctactt cttttaaatc taaagattta ggattggaaa caacatctga cattgttgct
     6421 aatgtatcac ctgatacttt caattttagc aagcctgctg attgtaacaa gtagagagag
     6481 cgactttcgt taactgcatc gtttgggata gtaatagttg ctttatttgg aatttcagaa
     6541 actgtttggt atttaccttt accgttctta gtaccagagt ataatctaaa ggaagtaaag
     6601 tatgtctcag caacggaaac aagattggtc ttatttgctt tattccaatt gtttaagtaa
     6661 ttataatgct ggaaagcatt aatatcaatc tcatcacttt ctaaagcttt attaggttgt
     6721 gtataatctg tgaattccgt aaattttagc ttaacgcctt ttttcttaac taattcttcg
     6781 attttatccc aacgagcttt ttcagtttta ctctttacca ttgttccaac tctaacgact
     6841 ttggcgtctt catttttaga ctgaccacag gctcctaaaa taagtgctga aattactact
     6901 gttgtcgtta accctaatag ctttttgatt ttcataattt atttctcctt tgatatggtt
     6961 gtatgattaa tcaactgata ctagtgtaac aaaaagctaa tggattaaaa aatacttatt
     7021 atcaatcaag agatatagct tttaactata tcatttcttt tataatttta ctttcgaggg
     7081 ctccgtaaag cggtccaata ataggcgact tcctcataac cttggtgctc atacattttt
     7141 ctgttttctt cttctgaaat taagataact ttcttaaacc ccctacttgc atcaaattgt
     7201 aggcgcgtac caattccttg tcctctgtag gactcttgga cataaaaatc atctatttcc
     7261 acgtattcac catagtccca agctgttaca tctccaataa tttgattatc tttaatagct
     7321 aggtaaactt tagcattatt atttaaaaga ttaacacgat tatcattata catttgctta
     7381 gcgtaaattt ctccgtaaac tatactttgt ttatatttga actttaggta tttttctagg
     7441 taattttcat taagtttagt aaatgtgatg tcatcaaatt ctttttcaaa aagtttaaga
     7501 tgatttaatg aatttgtaaa tatgatatgt ttctctgatt caaagccttg ttgtgataaa
     7561 gttctctgta attcactgct caattcagca ttttcctcaa aaaatacaaa agcaaaatca
     7621 gactgataat ctttttgttc atctgctaaa tagatcatat cctgtaaaaa ctcacttact
     7681 gtaggcatta ttttgtagct aatatagttt gaaaaataac gctctgaagt gattatattc
     7741 ttaaaatgtt tataccgatt agtttccaaa taagcttttt cggatgactc aagtaattct
     7801 ttatagtgca tatttactcc attcatacat ttttaaaaat caatatagaa aatttgacct
     7861 tgcttgttgg caaagtcaaa tgaatatatc aatagttgag aaatttttct aaatctttca
     7921 aagctcgtgt agcaatagct tcataacgct ctttcttatc acgaattcgt ggaccttcta
     7981 attctttgat gataccaaag ttaacattca ttggttggaa atgtttgctg tctgtatgtg
     8041 taatataatg tggcagtgca ccaatagcag tagtttgtgg gaaaactact tcactctccc
     8101 cattgaagcg acggacagca ttgattcccg ctacaagacc acttgctgcc gattctacat
     8161 aaccttcaac accggtcatt tgtccagcaa agaaaagatt tgggttctta cgtgtagcaa
     8221 aggtttggtt taaaagattt ggtgaatcca tataagaatt gcgatgcata acaccatacc
     8281 ggacaaattc agcattttca agaccaggaa tcatttgaaa tacacgtttt tgttctcccc
     8341 acttaagatg agtttggaaa ccaactatgt tataaagaga gcctgcagca ttatcttgac
     8401 gtaattgaac aacagcatat ggtgttttaa attcaccatc tcgcggtcct ttgtagtcct
     8461 caggatactc caaaccaact ggtttcattg gaccataaag catcgtttta ataccacgct
     8521 tagccatcac ttcgataggc atacaacctt cgaagtattt ttctttctca aaagaattta
     8581 gtggtgcttc ctcagctgta gttaaagctt catgaaaagc cataaattct tctttggtca
     8641 ttggacaatt tagataagct gcttcacctt tgtcatacct agacttaagg taaactttat
     8701 tcatgtcaat cgtattttta tcgacaattg gagcggcggc atcgtaaaaa taaaaaccat
     8761 caccaccatt aagttcatgg attttagctg ccagactatc acttgttaat ggtcctgtag
     8821 cgataactgt aatagcatct ccagggatat ctgtgatttc atcacgaatt acttcgatta
     8881 atggatgttt atggatttcc tcagtaacag cttcagaata accttctcta tcaacggcca
     8941 tggctccacc tgctggaact cgatgtgcct ccccatttct cataatgata gaatccaaac
     9001 gacgcatttc ttcttttaaa agaccaactg catttgttag agaatcaccg cggaatgaat
     9061 tggaacatac taattctgca aaattatctg ttttatgttg aggggttgat ttaaccccac
     9121 gcatttcata aagcttaact ggtattccac gtttagcaat ttgataagct gcttcagaac
     9181 cagccaaacc agcgccgata acattaatgt aagattgaga catgaactaa tacctcttga
     9241 aagtagtcaa ctacttcctc aaatctttct atataattta tttttccatt ataacataca
     9301 tagctacttc ttcaaaaaac gttattcatt aaactactag ttattctcta aataaaactc
     9361 aaaacgatct ccaacatatt gcccatgaac atattcaaaa ggcttaccat cagtaaagta
     9421 tgaaacttgt gtcaatgcca aaatagcatg gcctgcgtta acttctaaat gcgaagcaac
     9481 acgttcacta gcatttctag catatatagt ttgttggctt ttcccaattt cgtagccatt
     9541 ctctgttaag gttttaaaga aatgttctgt aacctctgat tgttcaaaac ctttaattaa
     9601 attctctggt atagaggcta cctcataaac caacggtacc ttatcagcat aacgtaccct
     9661 ttccatacga accacataat ctgactgtga taaatttaat ttttgaattt cggtttcatt
     9721 agctagtttt ctttgaaatg agattagttt actggatggc ttacgccctt gagaattgac
     9781 aatttctgta aaactagttg ttccccgcat tttttcttga acacgatgac tagcaacata
     9841 ggtgccacta ccaactcgtc tctccaaaat accttcttca actaacaatg taattgattg
     9901 gcgaagtgtc atacgactta cactataatc atccgctaaa tctctttcgc taggtaaccg
     9961 ctgcccaatc ttccaagtcc ctttatcgat ttccttttta atagcgtcgt gaattttaat
    10021 atatgctggt aacaatattc tctcctccta gaatctacac ttcattttac catacttcta
    10081 tatatttcta actctaaaat cgtgaaataa ttcagaaaaa tcataagcaa tatttcttg
//
LOCUS       DBJYSH010000073        10096 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.073, whole genome shotgun sequence.
ACCESSION   DBJYSH010000073 DBJYSH010000000
VERSION     DBJYSH010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 10096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10096
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.073"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            89..538
                     /locus_tag="ACUBV3_001425"
     CDS             89..538
                     /locus_tag="ACUBV3_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001039194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HHU3847923.1"
                     /translation="MNKNQYPKWEELPELDLYLDQVLLYVNQLINPKTITNDKLLTAS
                     MINNYVKHNYISKPIKKKYNRRQVARLIVITAFKQVFAIQEISQTLELLTADNHSEEA
                     YNGFAACMNKEEVHDLPPVVISACQTLNLYQETQKLVLELGGKNKNA"
     gene            531..1187
                     /gene="trhA"
                     /locus_tag="ACUBV3_001426"
     CDS             531..1187
                     /gene="trhA"
                     /locus_tag="ACUBV3_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000910676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAQR family membrane homeostasis protein TrhA"
                     /protein_id="HHU3847924.1"
                     /translation="MLKEMSIKLSPQLSFGEELANSITHAVGALLMLILLPITAVYSH
                     NHFGLQAALGTSIFVTSLFLMFLSSSIYHSMTYNSLQKYVLRMIDHSMIYIAIAGSYT
                     PVALSLIGGWLGYLIIFLQWGITLFGILYKIFAPKINDKFSLVLYLIMGWLVIFIFPA
                     IITKTGPAFWGLLLAGGICYTIGALFYARKRPYDHMIWHLFILLASILQYIGIVYFML
                     "
     gene            1274..2188
                     /locus_tag="ACUBV3_001427"
     CDS             1274..2188
                     /locus_tag="ACUBV3_001427"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol/lipid kinase family protein"
                     /protein_id="HHU3847925.1"
                     /translation="MKSAYIFFNPKSGKDEQALAKEVKSYLIEHDFQDDYVRIITPSS
                     VEEAVALAKKASEDHIDLVIPLGGDGTINKICGGVYAGGAYPTIGLVPAGTVNNFSKA
                     LNIPQERNLALENLLNGHVKSVDICKVNDDYMISSLTLGLLADIAANVTSEMKRKLGP
                     FAFLGDAYRILKRNRSYSITLAYDNNVRSLRTRLLLITMTNSIAGMPAFSPEATIDDG
                     LFRVYTMEHIHFFKLLLHLRQFRKGDFSQAKEIKHFHTNNLTISTFKRKKSAIPKVRI
                     DGDPGDQLPVKVEVIPKALKFIIPNSLP"
     gene            2289..3143
                     /locus_tag="ACUBV3_001428"
     CDS             2289..3143
                     /locus_tag="ACUBV3_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase C-terminal
                     domain-containing protein"
                     /protein_id="HHU3847926.1"
                     /translation="MTTFTAKFIDQEWEVPVESGRYHMIVGEFCPYAQRPQIARQLLG
                     LDKHISISFVDDVPSDIGLIFSQPEQVTGAKSLRDIYHLTDPTYQGPYTIPILIDKTD
                     NRIVCKESADLLRLFTTDFSDLHQEDAPVLFSQETASLIDNDIKDINKNFQSLMYKLA
                     FLDKQADYDTYSKEFFTFLDQKEHLLGQRPFLLGDNLSEVDIHFFTPLVRWDIAGRDL
                     LLLNQKALEDYPNIFSWAKTLYNDFNLKTLTNPQSIKNNYYLGKFGRAVRHHTIVPTG
                     PNMAKWEK"
     gene            complement(3189..3407)
                     /locus_tag="ACUBV3_001429"
     CDS             complement(3189..3407)
                     /locus_tag="ACUBV3_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HHU3847927.1"
                     /translation="MIKLYYKDAYDTERCQVFENPNDFIRVLMGCVTVPDNYPITKMT
                     YKDKDIAYSGTYGDLFKTFIEFDWSTLD"
     gene            complement(3475..4470)
                     /gene="fni"
                     /locus_tag="ACUBV3_001430"
     CDS             complement(3475..4470)
                     /gene="fni"
                     /locus_tag="ACUBV3_001430"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HHU3847928.1"
                     /translation="MTNRKDDHIKYALKYQSHYNSFDDIELIHSSLPKYNVNDIDLST
                     HFAGQSFEFPFYINAMTGGSEKGKAVNHKLAQVAQATGIVMVTGSYSAALKNDEDDSY
                     PTTDLYPDLKLATNIGLDKPVPAAESTVKAMNPIFLQVHVNVMQELLMPEGEREFHMW
                     RSHLKEYVDNIQCPLILKEVGFGMDLQSIKDAYDIGITTVDISGRGGTSFAYIENQRG
                     RDRSYLNTWGQTTAQSLINAQSMMDKMDILASGGIRHPLDMVKCLVLGAKAVGLSRTV
                     LELVERYPVDDVIAILNSWKEDLRMIMCALNCKKITDLRQVNYILYGQLKEANAK"
     gene            complement(4467..5459)
                     /locus_tag="ACUBV3_001431"
     CDS             complement(4467..5459)
                     /locus_tag="ACUBV3_001431"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004631 - phosphomevalonate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HHU3847929.1"
                     /translation="MVKVQTGGKLYIAGEYAILYPGQVAILKNVPIYMTALATFADNY
                     SLYSDMFNYTASLQPDKQYSLIQETILLMEEWLINFGKNIKPIHLEITGKLERYGLKF
                     GIGSSGSVVVLTIKAMAALYEIEMPSDLLFKLSAYVLLKRGDNGSMGDIACIAYEHLI
                     SYSAFDRRAVSKMIETKPLEQVLEAEWGYRITKIQALLEMDFLVGWTMQPSISKEMIN
                     IVKSTITQRFLDDTKYQVVQLLSAFKEGDKEAIKRCLEEISLLLFNLHPSIYTDKLQK
                     LKEASKGLDIVTKSSGSGGGDCGIAISFNKNDNQTLIKRWESAGIELLSKETLS"
     gene            complement(5452..6396)
                     /gene="mvaD"
                     /locus_tag="ACUBV3_001432"
     CDS             complement(5452..6396)
                     /gene="mvaD"
                     /locus_tag="ACUBV3_001432"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000348814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HHU3847930.1"
                     /translation="MDGKSISVKSYANIAIIKYWGKADAEKMIPATSSISLTLENMYT
                     ETRLTALGKDAKKDEFYISGVLQNDHEHDKMSAILDRFRQNRSGFVKIETTNNMPTAA
                     GLSSSSSGLSALVKACNDFFGTNLSQSQLAQEAKFASGSSSRSFFGPVAAWDKDSGDI
                     YKVHTNLDLAMIMLVLNDKRKPISSREGMKICTETSTTFNEWVRQSEQDYQDMLVYLK
                     NNDFQKVGQLTERNALAMHSTTKTATPAFSYLTEETYKAMDVVKKLREKGHECYYTMD
                     AGPNVKVLCLRQDLEALAAILEKDYRIIVSTTKELADG"
     gene            complement(6378..7256)
                     /gene="mvk"
                     /locus_tag="ACUBV3_001433"
     CDS             complement(6378..7256)
                     /gene="mvk"
                     /locus_tag="ACUBV3_001433"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HHU3847931.1"
                     /translation="MKEKFGIGKAHSKIILMGEHSVVYGYPAIAIPLKNIEVTCLIEE
                     APQLIALDMTDPLSTAIFAALDYLGKTSSKIAYHIESQVPERRGMGSSAAVAIAAIRA
                     VFDYFDEDLEADLLECLVNRAEMIAHSNPSGLDAKTCLSENTIKFIRNIGFSTVPMHL
                     NAYLVIADTGIHGHTKEAVDKVKSSGEAVLPFLKELGYLAEASEDAIHKSDSKQLGSL
                     MTKAHQSLKQLGVSSLEADHLVEVAISCGALGAKMSGGGLGGCIIALVKEKREAERLS
                     QQLEREGAVNTWTEKV"
     gene            complement(7372..8601)
                     /locus_tag="ACUBV3_001434"
     CDS             complement(7372..8601)
                     /locus_tag="ACUBV3_001434"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HHU3847932.1"
                     /translation="MFRNLRLRFIGIAALAILVVLFSVVGVLNSANHYQTKNEIYRVL
                     TILADNNGRIPNKLEFSKELGDDLSTDAIFQFRYFSARTDAKGNVTSFDSRNIFEVSD
                     RQIKNYAKRIVSQNSHSGHITYNFSTYSYLLKKVGKNDYLVVFLDTTNQYLDNQRLLQ
                     LSIWMSLVSFIVFMVIVSVLSGRVILPFVANYEKQRRFITNAGHELKTPLAIISANNE
                     LVEMMSGESEWTKSTNDQIQRLTGLINGMVSLARFEEQPDISMVDLDFSHITKDAAED
                     FKGPIIKDGKDFIMSIQPGIHVKAEEKSLFELVTLLVDNANKYCDPMGTVTVKLSRSS
                     RLRRAKLEVSNTYKNGKDIDYSKFFERFYREDESHNNKKSGYGIGLSIVTSLVHLFKG
                     SIDVNYKHDTITFVIYI"
     gene            complement(8601..9287)
                     /locus_tag="ACUBV3_001435"
     CDS             complement(8601..9287)
                     /locus_tag="ACUBV3_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HHU3847933.1"
                     /translation="MRVLIAEDEEQMSRVLSTAISHQGYVVDVAYDGQTAIDLANQNA
                     YDVMVMDVMMPVKTGIEAVKEIRQSGNKSHIIMLTAMAEIDDRVTGLDAGADDYLTKP
                     FSLKELLARLRSMSRRLEDFTPNVLSLGRVTLSVGEQELQCENTIRLAGKEAKMLAFF
                     MLNHDKELSTQQLFEHVWGADKDQEDVDEGYVFIYVSYLRQKLQAIGANLEIIGQEGS
                     SYKLSEINGG"
     gene            complement(9289..9915)
                     /locus_tag="ACUBV3_001436"
     CDS             complement(9289..9915)
                     /locus_tag="ACUBV3_001436"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HHU3847934.1"
                     /translation="METNIYGDYGRYLPLILEDFSQRIQLENDKAKVETGYKLYEHII
                     GRIKTSDSMIEKCRRKQLPVTVDSALKTIRDSIGVRIICGFVNDIYQIIERIKAFDDC
                     RIVVEKDYIQHVKPNGYRSYHVILEIDTPYPDCLGNSDGKYYIEIQLRTIAQDSWASL
                     EHQMKYKHDIENPERIVRELKRCADEMASVDLTMQTIRQLIESGTKKE"
BASE COUNT         3180 a         1991 c         1568 g         3357 t
ORIGIN      
        1 aaatcatctc aaattctttt tttacttgac ttgaatttcc ataagtgtat aatctagtta
       61 taaagactag attatatgga gttttaaaat gaataaaaat caatacccaa aatgggaaga
      121 attacctgaa ctagatttgt atcttgacca agtacttctc tatgtgaatc aattaattaa
      181 tcctaaaaca ataactaacg ataaactact caccgcttcg atgatcaaca attatgtcaa
      241 acataattat atatccaaac ctataaaaaa aaaatacaat cgccgtcaag tagcacgatt
      301 gattgttatt acagctttta aacaagtttt tgctattcaa gaaatcagtc aaacattaga
      361 acttctgaca gctgacaatc attctgaaga agcatataac ggatttgctg cttgtatgaa
      421 caaagaagag gttcacgatt taccaccagt tgtcattagt gcctgtcaga cgttgaacct
      481 ttatcaagaa acacaaaaat tagttttaga actaggagga aaaaataaaa atgcttaaag
      541 aaatgtctat caaattaagt cctcaacttt cctttggcga agagcttgca aatagtatca
      601 ctcacgctgt aggagctttg cttatgctaa tcctattacc aatcaccgct gtctatagtc
      661 acaaccactt cggattacaa gcagcacttg gtacctcaat attcgtcact tctttatttt
      721 tgatgttttt atcatcaagt atttaccact ccatgactta taacagtctt caaaaatatg
      781 ttctacgcat gattgatcac tctatgattt atattgctat tgcaggtagt tatactcctg
      841 tcgcattgtc acttattggc ggctggttag gatatctcat cattttttta caatggggga
      901 ttacattatt tggtatcctt tataaaatct tcgctcccaa aatcaatgat aaatttagcc
      961 ttgtacttta cctcattatg ggatggctgg ttatctttat tttccctgct attatcacaa
     1021 aaactggacc agctttttgg ggattactct tggctggtgg tatttgctat actatcggtg
     1081 cactctttta tgctcgcaaa cgtccttatg atcacatgat ttggcacctt tttattctat
     1141 tagcttctat tttacaatac attggaattg tctacttcat gctataaaat ttaactacat
     1201 tttattgatt atttttttgc taaaccatga tataataata atcatctaaa tcttagagag
     1261 aaggcgtgga catatgaaaa gtgcttatat cttttttaat ccaaaatctg gtaaagatga
     1321 acaagcattg gcaaaagaag taaaatctta tttaatagaa catgattttc aagacgatta
     1381 tgttcgtatt attacacctt catctgttga ggaagctgtc gccttagcaa aaaaagcatc
     1441 tgaagaccat atcgacttgg ttattccttt agggggagat ggcactatta ataaaatctg
     1501 tggtggtgtc tacgctggtg gtgcttatcc tactatcgga ctagtacctg ccgggaccgt
     1561 caataacttt tcaaaagctt tgaacatccc gcaagagagg aatctcgctc ttgaaaacct
     1621 tctaaacggt catgtaaagt ccgtagatat ctgtaaagtt aatgacgatt acatgattag
     1681 cagtttaaca cttggtctcc ttgctgatat tgctgctaat gtcacttctg aaatgaagcg
     1741 taaattaggc ccttttgctt tcttaggtga tgcctaccgt atattaaagc gtaatcgatc
     1801 atattctatc actcttgctt acgataataa tgtacggtct ttacgcacgc ggttactcct
     1861 tattaccatg accaattcga ttgccggtat gccagccttc tctccagaag caacaattga
     1921 tgatggactt tttagagtct acactatgga acatattcac ttcttcaaat tattgttgca
     1981 tctaagacaa tttcgtaagg gagattttag tcaagcaaag gagataaaac attttcatac
     2041 caataatctg actatttcaa cttttaaacg taaaaaatca gctatcccta aagttcgtat
     2101 tgatggcgat ccaggtgacc aattacctgt aaaagttgaa gtcatcccta aagccttaaa
     2161 gtttatcatt cctaactcat taccataata taaaaggacc tagacataag tattagagta
     2221 aatactatct gtctaggttc ttttttgtta tacttaaagc aataaatatc attgaaagga
     2281 agtatacgat gacaacattt actgctaaat ttattgacca agagtgggaa gtcccagtag
     2341 aatcaggtcg ctaccatatg attgttgggg aattttgtcc ttatgcacaa cgtccgcaga
     2401 ttgcgcgcca actgcttggt ttggataaac atatttctat cagttttgtg gatgatgttc
     2461 caagtgatat aggacttatt tttagccaac cagaacaggt tactggtgct aagtcgcttc
     2521 gtgatattta tcatttgact gacccgacct accaaggtcc ttatactatc ccaattttaa
     2581 ttgacaagac cgataatcgt attgtctgta aggaatctgc agatttgctt cgacttttta
     2641 ctacagattt ctctgatttg catcaagagg atgctcctgt ccttttcagc caagaaactg
     2701 cttcacttat tgataatgac attaaagaca ttaataagaa cttccaaagc ttaatgtata
     2761 agttagcttt tcttgataaa caagcagatt atgatactta tagtaaggaa ttcttcactt
     2821 ttctagatca aaaagaacac ttactaggac aacgtccttt cttgctcgga gataatctaa
     2881 gtgaggttga tattcatttc tttacaccat tagtccgatg ggatattgct gggcgtgatc
     2941 ttctactact taatcagaaa gcgctagaag actacccaaa tattttttct tgggctaaaa
     3001 ctttatacaa tgactttaat ttaaaaacat taaccaatcc acaatccata aaaaataact
     3061 attacttagg caaatttgga agagctgttc gtcatcatac catcgtccca acaggtccaa
     3121 atatggcaaa atgggaaaaa taaagctaaa aagctaaggc aattcacagc cttagctttt
     3181 tagctatact agtccaaagt ggaccaatca aattcaataa aagtcttgaa gagatctcca
     3241 taagtaccac tgtaagcaat atctttatct ttataagtca ttttcgtaat aggataattg
     3301 tcaggaacag taacacatcc cattaaaact cgaataaaat catttggatt ttcaaaaact
     3361 tggcatcgtt cagtgtcgta agcatcttta taatacagtt taatcatctt attacctctg
     3421 acgttttaca aggaaacttt gacaggaagc tatatctact tcacaaaaat aatatcattt
     3481 agcattggct tccttcaatt gaccatacaa tatataattg acttgtcgta aatcagttat
     3541 ttttttacaa ttcagtgcac acattatcat tcttaaatct tctttccaag aattgagaat
     3601 tgcaataaca tcatctacag gataacgctc aactaattct aaaactgttc tagaaagtcc
     3661 aacagcttta gcacccaaca ctaaacattt taccatatct aaaggatgac gaatgccacc
     3721 agatgctagg atatccattt tgtccatcat agattgagcg tttattaagg attgagctgt
     3781 tgtttgcccc caggtattga gatacgaacg gtcgcgtcca cgttgatttt cgatgtaagc
     3841 aaaactagtc cccccacgcc ccgaaatatc tacagttgtg atacctatat cgtaagcatc
     3901 ctttattgat tgtaaatcca tcccaaaacc aacctctttt aaaataagtg ggcattggat
     3961 attgtcgacg tattctttta agtgagaacg ccacatgtga aattcacgct ctccttcagg
     4021 catcaaaagt tcttgcatca cattaacatg aacttgtaaa aaaatggggt tcatagcctt
     4081 aacggttgac tctgctgctg gtacaggctt gtctaaccct atatttgtcg ccagttttaa
     4141 atcaggatac aagtctgttg ttgggtagga atcatcttca tcgtttttga gagctgccga
     4201 atatgaacct gtcaccataa caatccctgt agcttgagca acctgtgcca atttatgatt
     4261 gactgctttc cctttttcac taccgcctgt catagcattg atataaaaag gaaattcaaa
     4321 gctttgacca gcaaaatgtg ttgataaatc gatatcgttg acattatatt ttggtaagga
     4381 tgaatgtatt aactcaatat cgtcaaaact attataatgc gactgatatt taagagcata
     4441 ttttatgtga tcatcttttc gatttgtcat gacagtgtct ccttactaag tagttcaata
     4501 ccagcactct cccaacgctt gattagagtt tgattatcat ttttattaaa ggatatggct
     4561 ataccacaat cgcctcctcc agatcctgaa gattttgtga ctatatctag gcctttgctt
     4621 gcctctttaa gcttttgaag tttatcggtg taaatagatg gatgaagatt aaataatagc
     4681 aagctaattt cttctaaaca tctcttaatc gcttctttat ctccttcttt aaaggctgaa
     4741 agtaactgga caacttgata ctttgtatca tctaaaaaac gctgcgtaat agttgattta
     4801 actatattta tcatttcttt tgaaatggac ggttgcattg tccatcccac taaaaaatcc
     4861 atctccaata aagcttgtat tttggtaata cgataacccc attcagcttc caatacttgt
     4921 tccaaaggtt ttgtttcaat catttttgaa acagccctac gatcaaaagc tgaataagaa
     4981 attaaatgct cataagcaat acaagcaata tctcccattg agccattatc acctcttttt
     5041 aagagaacat aggcagataa tttaaaaagc aagtcgcttg gcatctctat ctcataaagc
     5101 gcagccatag ctttaatagt caaaacgaca acacttccgc tagagcctat accaaattta
     5161 agtccatagc gctctaattt tcccgtaatt tcgaggtgaa tcggttttat atttttgcca
     5221 aaattaatca accattcttc cataagcaat atcgtttcct gaatgagact atattgctta
     5281 tctggctgta agcttgccgt atagttaaac atatcagaat aaaggctgta gttatctgca
     5341 aaagtagcta atgcagtcat ataaatagga acgttcttta aaatagctac ttgcccagga
     5401 tacagtatcg cgtattcacc tgcaatatat aatttgccac ctgtttgcac cttaaccatc
     5461 tgctaattcc tttgtcgttg aaacaataat acgataatct ttctccaaga tagcagctaa
     5521 tgcctcaagg tcttgacgta agcaaagtac tttaacattt ggacccgcat ccatcgtata
     5581 ataacattca tgaccttttt cgcgaagttt cttcacaaca tccatagctt tatatgtctc
     5641 ttctgtcaaa tatgaaaagg ctggagttgc tgttttagtt gttgagtgca ttgcaagtgc
     5701 attacgctct gtcagttgac ctaccttttg aaaatcatta ttttttaggt atactaacat
     5761 atcttgataa tcttgttcag attgccttac ccattcatta aaagtagttg aagtttcggt
     5821 gcagattttc ataccttcac ggcttgaaat cggctttctt ttgtcattta agaccagcat
     5881 aatcatagct aaatctaaat tagtgtgaac tttatagata tcaccactat ctttatccca
     5941 agcagcaaca ggtccaaaaa aagagcgtga agaggagcct gaagcaaact tggcttcttg
     6001 agctagctgt gattggctaa ggttagtacc aaaaaaatca ttgcaggcct ttactaaggc
     6061 tgataaacca cttgatgacg atgataagcc tgcagctgtt ggcatattat tagttgtttc
     6121 aatctttaca aagccggacc tattttgtcg aaagcgatcc aagatagctg acattttgtc
     6181 atgttcatga tcattttgaa gaacaccaga gatgtaaaat tcatctttct tagcatcctt
     6241 cccaagggca gtcaaacgtg tttcagtgta catgttttca agtgttagag aaatactact
     6301 ggtagctgga atcatttttt cagcatctgc ttttccccaa tatttgataa tagcaatgtt
     6361 agcataagac ttaacactta tactttttcc gtccatgtgt tgacagctcc ttctctttct
     6421 aattgctgac ttaagcgctc agcttctctt ttttctttta caagtgcgat aatacaacca
     6481 cctaatccgc cgccactcat tttagcgcct agagcaccac aactaatagc tacctctacc
     6541 agatgatcag cttctaaact tgagacaccc aattgtttta aactttgatg tgctttagtc
     6601 atcaagctac caagttgttt ggaatcactt ttatgaatag catcctctga tgcctctgct
     6661 aaataaccta attcttttaa aaagggtagt actgcctcgc cagatgattt caccttatcc
     6721 actgcttctt tagtatgccc atgaattcct gtatcagcaa taactaaata agcattcaaa
     6781 tgcatgggta ctgttgaaaa accaatattg cggataaatt taatagtatt ttcactaaga
     6841 caagtttttg catctaatcc actcggattt gaatgtgcta tcatttctgc tcgattaaca
     6901 aggcattcta acaaatctgc ctccaaatct tcatcaaaat aatcaaaaac tgcacgaata
     6961 gctgcaatag caactgcagc tgatgatccc ataccacgtc tttctgggac ttgtgactca
     7021 atatgatatg caattttaga agaagtttta cctaaatagt ccaaggcagc aaaaatggct
     7081 gtcgacaaag gatcagtcat atctaatgct attaattgag gagcctcttc aataaggcaa
     7141 gttacttcaa tattttttaa aggaatagca atggctggat aaccatatac tactgagtgc
     7201 tcacccatca agataatctt gctatgagcc tttcctatac caaacttttc tttcatcttt
     7261 ttctctttac aattctatta ccttactatt ttaatataaa tccttaaaaa aagcgacttt
     7321 aacgtgataa taacataaat taaaggaact acaattgcag tccctttaat cttaaatgta
     7381 aatgacaaag gtaatggtat catgtttata attcacatcg atacttcctt taaatagatg
     7441 caccaaacta gttacgatag aaagaccaat accatatccc gattttttgt tattatgtga
     7501 ctcatcctct cgataaaatc gttcaaaaaa cttgctatag tctatgtctt taccattctt
     7561 ataagtattt gaaacttcaa gcttagctcg tctcaaacga cttgaacgtg ataacttaac
     7621 tgtcacagtc cccataggat cacaatactt attagcatta tcaaccaata atgtaaccaa
     7681 ttcaaataat gatttttctt cagctttgac atgaatacct ggttggatac tcattataaa
     7741 gtctttacca tctttaatga ttggaccttt aaaatcttct gcagcatctt ttgtaatatg
     7801 tgaaaagtct aagtcaacca tacttatatc aggctgttct tcaaagcggg caagactgac
     7861 catgccatta attagacccg ttaaacgttg gatttgatca tttgtagatt tagtccactc
     7921 tgattcacca ctcatcattt ccaccaattc attattagcg gagatgatag caagtggagt
     7981 ttttagttca tgtcctgcat tggtaataaa acgcctttgc ttttcatagt tagcaacaaa
     8041 tggtaagata actctccctg ataaaactga aacaattacc atgaagacga taaaacttac
     8101 caaggacatc catattgaaa gttgcaataa tctttgatta tcaagatatt ggttggttgt
     8161 atctaaaaat accactaaat aatcattttt accaaccttt tttagtagat aagaataagt
     8221 actaaaatta taggtgatat gacctgaatg actattttgt gatacgatac gcttggcata
     8281 attttttatt tgcctatcgc ttacttcaaa gatatttcta gaatcaaaac tagtcacgtt
     8341 accttttgca tctgttcgcg cactgaaata acggaattgg aaaatagcgt ccgttgatag
     8401 gtcatcacct aattctttac taaattcaag tttattcggt atacgtccat tgttgtctgc
     8461 aaggatagtt aacacacgat aaatttcatt cttcgtttga tagtgattag cagagttgag
     8521 gacaccgact actgaaaata agacaacaag aatagctagc gcagctattc cgataaaacg
     8581 aagtcgtaaa tttctaaaca ttagccacca tttatttcac ttaatttata gctactacct
     8641 tcttgaccaa taatctccaa gttagcacca attgcctgca atttttgtct caaataagaa
     8701 acatagataa agacgtatcc ctcatctaca tcttcttggt ccttatctgc tccccagaca
     8761 tgttcgaaaa gttgttgtgt agataattct ttatcatggt tcaacatgaa aaatgctaac
     8821 attttagctt ctttacccgc tagacgaata gtgttttcac attgcaactc ttgttcaccg
     8881 actgataagg ttactcttcc caatgacaat acattaggtg tgaaatcttc caatcttcgg
     8941 gacatagagc gaagccttgc taataattct tttagagaga agggttttgt taaataatca
     9001 tctgcaccag catctagacc tgttactcta tcatcaattt ctgccatggc tgttagcata
     9061 ataatgtgag atttatttcc tgattgtcta atttctttga cagcctcaat acctgtttta
     9121 acaggcatca tgacatccat aaccatgaca tcatatgcat tttgattagc taaatcaata
     9181 gcagtctgac catcgtaggc gacatcaaca acataacctt ggtgactaat tgccgtacta
     9241 aggacacgtg acatctgttc ttcatcttct gcaattaata ctctcatttt attccttctt
     9301 tgttccactc tctatcaatt gacgaatcgt ttgcatcgtt aaatcaacgg atgccatttc
     9361 atcagcacaa cgttttaatt cccttacaat tcgttcggga ttttcaatat catgcttgta
     9421 tttcatttga tgttctaaac tagcccaaga atcttgcgca atggtacgca actgaatttc
     9481 aatgtaatat ttaccgtctg aattacccaa acagtctgga tagggggtat caatttctaa
     9541 aatcacatga taagaacgat acccatttgg cttaacatgc tggatataat ctttttcaac
     9601 cacaatacga caatcatcga atgccttaat acgttctata atttgataaa tgtcgttaac
     9661 aaaaccgcaa ataatacgga ctccaatgct atctctaatc gtttttagtg cagaatctac
     9721 cgttacaggt aactgtttgc gacgacattt ctctatcata ctatctgacg ttttaatacg
     9781 accaatgata tgttcgtaca atttatagcc tgtttcaacc ttagctttat cattttctaa
     9841 ttggatacgc tggctgaaat cttctaaaat taatggtaga tatcgaccat aatctccata
     9901 aatatttgtt tccataagaa accttcctat tctctcttag cctccattat atcatagaaa
     9961 cttaaggcaa actaaaaaga tggtaggtac tgtgagacta tttatacaaa taattgttac
    10021 ttaaggatct taccacaata atttgtaaaa tttattgagt ttgtcaagca aaggcagtta
    10081 taaagttaag ataatt
//
LOCUS       DBJYSH010000074         9722 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.074, whole genome shotgun sequence.
ACCESSION   DBJYSH010000074 DBJYSH010000000
VERSION     DBJYSH010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 9722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9722
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.074"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            82..423
                     /locus_tag="ACUBV3_001437"
     CDS             82..423
                     /locus_tag="ACUBV3_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3847935.1"
                     /translation="MSEFLSAKPIGHNDSSNTILRIIENTKYIHHYDGKVVVEIPPQK
                     EPIKVFFMDSRLHYLEFSDICRYVTVVLKNSTVDNITNLGLDNHIVYDNGDDMVDPLP
                     SIQHGLFLNDD"
     gene            457..972
                     /locus_tag="ACUBV3_001438"
     CDS             457..972
                     /locus_tag="ACUBV3_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cross-wall-targeting lipoprotein signal
                     domain-containing protein"
                     /protein_id="HHU3847936.1"
                     /translation="MTFYKKTDKFGFRKSKVCRSLCGALLGTVAVVSLATASTEIHAD
                     EATTSPTTVTKVPQPVQADTTALNTSKTHSTQATTTPVEAKENKVVKSETVQSESRVM
                     PRDKVVERPETVKASVNSDVSQPITTTPPTINEKTVEIPNLAQDTKKVAPKVTVTPEV
                     VNQPKQLPKYE"
     gene            1024..3681
                     /locus_tag="ACUBV3_001439"
     CDS             1024..3681
                     /locus_tag="ACUBV3_001439"
                     /inference="COORDINATES: protein motif:HMM:NF012311.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HHU3847937.1"
                     /translation="MTKPQGAVAEKATPAVPKPQKVRVIVEFNKESILDYATEQKKTV
                     AQLNQADVEKKLQSIKQEQDKVLKNIEKSVHFDSSKVKRYDAIINGVALDIQAQEIEK
                     LKTIADVRRVYVSQEYVQTKPLLSSSGQLIGLPEVWNNSQYKGEGTVVAVIDSGVDFK
                     HQALKIKEPNRAKYNKTSIEKLIHEKNLKGKFYSEKVPYGYNYYDYNDNLKDSYGVMH
                     GMHVTGIVGANDDNQKLYGVAPNAQILAMKVFSDDQQNPTTFTDVWLKALDDAILLKA
                     DVVNMSLGTPAGFVHEGKDYPELEVIARACKAGIVIAVAAGNEGNITDGNTYGVKPLA
                     ENYDTALIANPALDDNTLAVASMENLKKHAHVLKFKDKKSGTEVTEVINLHVAPNASK
                     TIIGLAVDLGAEAPSELSKHFDLSGKIAMLEIPEDNKSNGFLEKVQAITKLNPAAILL
                     YNNAKVKDDLGSQLLVESEAAKFNIARITRSTYNNIKNNSNKIITILTERQAIDNSLA
                     GQLSSYSSWGPTPDLRLKPEITAPGGHIFSTVEDNQYADKSGTSMAAPQVAGAAAVLK
                     QYITDKKIPVDNAADFIKLLLMNTAQPIINKQSKDGKTPYFVRQQGSGAMNLAKALVT
                     TVVATVTGTNDNNADGKLELRELKEKKFKARILLRNFGKTNKTYIISSEAIADPVDEK
                     GFRTQNSEHLVSKKADAVTRKVTVEAGKTLAVDLDVDYSDAEALTRNNFLEGYLNLKD
                     TEGVADLHLPFLGFYGSWTEQKAIDAFEGISEIGNGDKKRRVQFYVNKETNKTSSTFT
                     TNGMLSLPIYNNTVFFSPNSPFYDKAGVRIAALRNMEYVQYSIIDPDTNKEVRVLGRS
                     HDVRKLYRLDYRNSFAMMPDSIWDGKIKD"
     gene            3727..7044
                     /locus_tag="ACUBV3_001440"
     CDS             3727..7044
                     /locus_tag="ACUBV3_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001060267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3847938.1"
                     /translation="MNNKGVGGDGVQIYQYYIKMDNNKPYLSPKDKTTVEKLEDRWKK
                     ITFKVQDTGIGLKDVYLQSVKYVGGGNNNLDLITPPGFKKEDKKVEKPKLDRPPGIDL
                     PAPTSMRSFDYSTPPGTKPSKPKDSLSTPPGFPDLNTPPDEAPKDSKKDAIEDKSGAI
                     KYAKSLQLSFVDDPILASKVNGKILQVESDGKLVIPRNALSANQFDDTSLKIYRNNNR
                     NKEITITTDYFADTKYVNITAVDYLSNTTFEQLATGETVDYHAIVFSSFAAIKDKGGK
                     IYVNDKLQETSRIALKDKSVKIGIELPNDVRHIDSLSVRRLNEVKTVDNILKNDEQDI
                     NLSKTYQLKYNPTNRRLEFTINNINSSSEIMTTFKDGKMPELVEQKDVSLDINDMDMS
                     KFKTIRLGRKDSEFKGQLIAKTGTVELDMFFKQSQDPASIIKKIYLIQNGVPNELKKF
                     DSSFGLTESQIDGYYIYKDAINLKFKLTSGASLKVVYKGQEDPYSHQKEDMTKKGEQL
                     SHSTQANENTAKVTFANIDWSHYSKVTVNGKEVGKGSELPLTKGWTTFVLHKTENSLN
                     VKSLIMETGSVSKKVQQLPLSPRLSKNKHMRDMLLTMQKDSAYYETSDSLVLRINLTA
                     DTKLNFNAVKGASALTENMMMRQFAVAGPQDDPVSEHKYPSVFLLTPALLETASEATL
                     NGKEITASGIIGHIKDGDKSKHVEVKMVNENGDMLGTPVIIQGKDLTNRTKPLMSGRR
                     VLYAGKQYEFRAKLPLSRFNTWIRVEVVTEAGEKASIVRRMFFDQSVPELNTAVAKRD
                     LTSDTALIHIVAKDDSLKLKLYQDDSLLESVDKTGLYSFRNGVEITKDMTVPLEFGDN
                     IIKLSAVDLSNYRRNETLHIYRNRFDVKASQMTADKGAKVTVDMLMKHLVVPEMAGAY
                     TLTIDEAPNTNESGMLTNAKVSIHYVNGGVDKVDVPIKVVDLEAIRKAEEAHKADEAR
                     KAEEARKAEEAHKAEEVRKAEEAHKVEEARKAEEGHKTQEAPIVEEGYKVNNVHQTDT
                     TVKASDLPKTKTVSAVHMARTDNKQITSHQTHVEKQIKNTLPSTGDSKRGYYITGMAI
                     VMLSVLFSLAKKFKSKY"
     gene            7228..8313
                     /locus_tag="ACUBV3_001441"
     CDS             7228..8313
                     /locus_tag="ACUBV3_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001873818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M13"
                     /protein_id="HHU3847939.1"
                     /translation="MQRKSYLKSMSVLTLTACLISGYVVKDIAMLHAVSASEKKANNV
                     SPRENLYRAVNDNWLANTKLKQGQTSVNSFSEIEDKLKQLLVSDMAKMASGKIETTND
                     EQKKMVAYYKQGMDFKTRDKNGLKPLKPVLQKLEAVSSMKDFQSLAHDFVMSGFVLPF
                     GLTVETNARDNSQKQLVLRQAPALLESPDQYKKGNKEGEAKLSAYRTSAMALLKQAGK
                     SNIEDRKLVKQAIAFDRLLSEKTQVDQSKITAESETAAGRYNPESMETVHNYAKEFDF
                     KELIEKLVGPTNKAVNVEDKTYFKQVNDVINSKQLANMKAWMMISMLVDQSDFLGEQN
                     RQAASAFKNVASGLTQIESKEKMLTPN"
     gene            8334..9152
                     /locus_tag="ACUBV3_001442"
     CDS             8334..9152
                     /locus_tag="ACUBV3_001442"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013590.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HHU3847940.1"
                     /translation="MGDYYGKKYFGEAAKKDVEHMAKKIINVYKTRLKNNTWLSENTK
                     AMAIKKLDNMRLMIGYPEDYPDLYRQYQFDSKASFFENNDNYRKLSNKKTFEEFNQSN
                     QREHWQMSANAVNAYNDPNTNSIVFPAAIFQSPLYDKTKTVSQNHGAIGAIIGHEISH
                     SFDINGMKYDEKGNLHDWWTKEDLKHYKKKTQAMIDQWDGLKADGGKVDGKLTLAENI
                     ADNGGVMASLEALKTEKIQTIKNFLNHGQVFGVKKQPKNKVSPQFSQMFMHHMN"
     gene            9077..9259
                     /locus_tag="ACUBV3_001443"
     CDS             9077..9259
                     /locus_tag="ACUBV3_001443"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013590.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HHU3847941.1"
                     /translation="MWRQKATKEQSKSSIQSDVHAPYELRANIPVRNFQEFYDAFGVK
                     KGDSMYLKPEKRLTLW"
     gene            9291..>9722
                     /locus_tag="ACUBV3_001444"
     CDS             9291..>9722
                     /locus_tag="ACUBV3_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HHU3847942.1"
                     /translation="MKNITKLSTVALSLLLCTACAASNTSTSKTQSHHPKQTKLTDKQ
                     KEEPKNKEAADQEMHPQGAVDLTKYKAKPVKDYGKKIDVGDGKKMNIYETGQGKIPIV
                     FIPGQAEISPRYAYKNLIERLSKKYKIYTVEPLGYGLSDIPT"
BASE COUNT         3582 a         1569 c         1838 g         2733 t
ORIGIN      
        1 ttgccattga caagtactta gaggagtgct aaaatttcgt tagctggtta acaaaccggt
       61 taagtaatgt gaggttcttt tatgagtgaa ttcctttcag ccaaacctat tggtcataat
      121 gattcatcta atactatttt aagaattata gagaatacga aatatattca tcactatgat
      181 ggaaaagttg ttgtcgaaat tcctcctcaa aaagagccta tcaaagtctt ttttatggat
      241 tcacgtcttc attatttaga attttcagac atatgtcggt atgtaactgt tgttttgaaa
      301 aatagtaccg ttgataatat aaccaattta gggctagata atcatattgt ttatgacaat
      361 ggtgatgata tggttgatcc gttgccgagt attcagcatg gattatttct taatgatgat
      421 taaagatgta gacaaaaatt tagaaacgag gaagccatga cattttataa aaagacggac
      481 aaatttggat ttaggaaaag taaagtgtgc cgcagcctat gcggtgcttt attgggtaca
      541 gtagcagttg ttagtctagc tacagcaagt acagagattc acgctgatga agcaacaacc
      601 tcacctacca ctgtaacgaa agtacctcag ccagttcaag cagacacaac agctctaaat
      661 acctctaaga ctcacagtac acaagcaact acaactcctg ttgaagctaa ggagaataag
      721 gttgtcaaaa gtgaaactgt tcaatctgag agccgagtaa tgcctagaga taaggtagta
      781 gaaaggccag aaacagtaaa agctagtgtt aactcagacg ttagccaacc tattacaacc
      841 acacccccaa caattaatga aaaaacagtt gagattccta acttagctca agatactaag
      901 aaggtagctc caaaagtaac tgttacacca gaagttgtta atcagccaaa gcagttgcct
      961 aaatacgagt aagaaaactc agagaaaggc aataagagta cagaagcaac taaggcagtt
     1021 actgtgacta aaccacaggg ggcggttgct gagaaagcta ctccggctgt gccaaagcct
     1081 caaaaagtaa gagtaattgt tgaatttaat aaggaatcta tcttagatta tgcgacagaa
     1141 cagaaaaaaa cagttgcaca gttaaatcaa gctgatgttg agaaaaaatt acaaagcatc
     1201 aagcaagagc aagataaagt tcttaagaat attgaaaaaa gtgttcactt tgattcaagc
     1261 aaagtgaagc gttacgatgc tattattaat ggtgtcgcac ttgatattca agctcaagaa
     1321 attgaaaaat taaaaacgat tgctgatgtt agaagggtct atgtctcaca agaatatgtt
     1381 cagacaaaac ctttactatc ttcctcaggg caacttattg gtttgccaga agtttggaac
     1441 aactcccagt ataaaggtga gggaactgtt gttgcagtta ttgattcagg ggttgatttc
     1501 aaacaccaag cactaaaaat caaagaacca aatcgtgcta agtataataa aactagtatt
     1561 gaaaaactga ttcatgaaaa gaatctcaaa ggaaaattct attccgaaaa agtaccttat
     1621 ggttataact attatgacta taatgataac ctcaaggata gctacggtgt aatgcatggt
     1681 atgcatgtaa caggtatcgt aggagctaat gatgataatc aaaaattgta tggtgttgca
     1741 ccaaatgctc aaattttagc gatgaaggta ttttcagatg accaacaaaa tcccacaacc
     1801 ttcacagatg tgtggctaaa agctctagac gatgctattt tactaaaagc tgatgttgtt
     1861 aatatgagtc ttggaacacc tgctggattt gtacatgagg ggaaagacta tcctgaactt
     1921 gaagttattg caagagcgtg taaggcagga atcgtcattg cagtagcagc aggtaatgaa
     1981 ggaaatatca ctgacggtaa tacttatggt gttaagccgc tagcagaaaa ttatgataca
     2041 gccttgattg ccaacccagc cctcgatgat aataccttgg ctgttgcctc tatggagaac
     2101 cttaaaaagc atgctcatgt tttgaaattt aaagataaaa aatctggaac agaagttaca
     2161 gaagtcatta atcttcatgt tgccccaaac gcttcaaaaa ctattattgg attggctgta
     2221 gatttgggag cagaagctcc ttcggaattg tcaaaacatt ttgatttaag tggtaaaatc
     2281 gccatgcttg aaattccaga agataataaa tctaatggat ttttagaaaa agtacaggcg
     2341 attactaagt taaatccagc tgctatcttg ctttacaata atgcgaaggt taaagatgat
     2401 ttaggcagtc aattattagt tgaatcagaa gcagctaaat ttaatattgc tcgcattacc
     2461 agaagcacgt ataacaatat caaaaataat agcaataaaa ttattactat tttaacagag
     2521 cgtcaagcta ttgataatag tttagcagga caattatctt cttattcttc ttggggacct
     2581 acaccagact tacgtctaaa acctgagatt acagctccag gtggtcatat tttctcaact
     2641 gtggaggata atcaatatgc tgataaatca ggaacatcaa tggctgcacc tcaggtagct
     2701 ggggcagctg cagttttaaa acaatacatt acagataaaa aaattcctgt tgataatgct
     2761 gctgatttta ttaaattact cttgatgaac actgcacagc caattatcaa taaacaatca
     2821 aaagatggca aaacacctta ctttgtgcgc caacaaggta gcggagcaat gaatcttgcc
     2881 aaagctcttg ttacgacagt cgttgcaact gtaacaggga ctaacgataa taatgcagat
     2941 ggtaagttgg agttacgtga attaaaagag aaaaaattta aagcacgcat tctacttcgt
     3001 aactttggaa aaactaataa aacgtatatc atctcatcag aagcgattgc ggacccagtt
     3061 gatgaaaaag gatttcgtac gcaaaattca gaacacctag tatcaaaaaa agctgatgca
     3121 gttacaagga aagttactgt agaagcaggt aagacattag ctgttgacct tgatgtggat
     3181 tattctgatg cagaggcttt aactcgcaat aatttcttgg aaggttatct taacttaaaa
     3241 gatactgaag gtgtagccga tttacactta ccatttttag gtttttacgg ttcatggaca
     3301 gagcaaaaag ctattgatgc ttttgaaggc atttctgaaa ttggtaacgg tgacaaaaaa
     3361 cgtcgcgttc aattctatgt taataaagaa accaacaaga cctcatcaac ctttacaact
     3421 aacggcatgt tgagtcttcc aatctataac aatacagtat ttttctctcc taatagtcct
     3481 ttctatgaca aagcaggagt tcgtattgca gctttgcgta atatggagta tgttcaatat
     3541 tctattatag atccagatac caataaagaa gttcgtgtac ttggtagatc acacgacgtt
     3601 agaaaacttt atcgcctaga ttatcgtaat agttttgcca tgatgccaga ttctatttgg
     3661 gatggtaaga ttaaagatta aattgcaaaa ggggataaac aatacattta tcaaattaaa
     3721 gtacagttga ataataaagg tgtcggtggc gatggtgtcc aaatttatca atactatatc
     3781 aaaatggaca acaataaacc ttacttaagt cccaaagata agactactgt agagaagtta
     3841 gaagatcgct ggaaaaaaat tactttcaaa gttcaggata ctggcattgg tttgaaagac
     3901 gtttatcttc aatctgttaa gtatgttggt ggtggcaata ataatttaga ccttatcaca
     3961 cctccaggat ttaaaaaaga agataaaaaa gttgaaaaac caaaattaga ccgtccacca
     4021 ggaattgatt taccagcacc aacttcaatg agaagttttg attattcaac cccaccggga
     4081 actaagccaa gcaaacccaa agatagttta tcaactcctc caggtttccc agatttaaac
     4141 acgccgccgg atgaagcacc aaaggatagt aaaaaagacg ctattgaaga taaatcagga
     4201 gcaattaaat atgctaagtc tcttcaactt agctttgttg atgaccctat tttagctagc
     4261 aaagtaaatg gcaaaatatt acaagtcgaa tctgatggca aattagtcat tcctagaaat
     4321 gctttgtcag ctaatcaatt tgatgacact agtcttaaaa tttatcgtaa taataatcgc
     4381 aataaagaaa ttactatcac aacagattat tttgcagata caaaatatgt caatatcaca
     4441 gcggttgact atttgagcaa tactactttt gagcaattag ctactggtga aacagtagat
     4501 taccatgcca ttgtattttc aagctttgct gctattaaag acaagggtgg taagatttat
     4561 gttaacgata aattgcaaga aacttctcgt atagcgctta aagataaatc tgttaagatt
     4621 ggtattgaat taccaaatga tgtcagacat attgatagtt tatctgttcg tcgtttgaat
     4681 gaggttaaaa ctgttgataa tatcttgaaa aatgatgaac aagacattaa tctcagcaaa
     4741 acttaccaat taaaatacaa cccgacaaat cgtcgtctag agtttactat taataacatt
     4801 aactcaagtt cagaaatcat gaccactttc aaagatggaa agatgccaga attggttgaa
     4861 caaaaagatg tttctttgga tataaacgat atggacatga gtaagtttaa aactattcga
     4921 cttggacgaa aggattctga atttaaggga caacttattg caaaaactgg aacagttgaa
     4981 ttagatatgt ttttcaaaca atctcaagac ccagcttcaa ttattaaaaa aatatacctt
     5041 atccaaaatg gtgttccaaa tgaattgaaa aaatttgact ctagttttgg tttaactgaa
     5101 agtcagatag atggatacta tatttataaa gatgcaatta accttaaatt taaattaacc
     5161 agtggtgcaa gtcttaaagt tgtttataaa gggcaagaag atccatatag tcatcagaaa
     5221 gaagatatga ctaaaaaagg tgaacagctc agtcattcaa ctcaagccaa tgaaaataca
     5281 gcaaaagtaa cctttgctaa tattgactgg tcacattata gtaaggttac tgtgaatgga
     5341 aaagaagttg gtaaaggtag tgagttacct ttaactaaag gatggacaac atttgtatta
     5401 cataaaacag aaaattcatt aaatgttaaa agtttgatta tggagacggg tagtgtaagt
     5461 aagaaagttc aacaacttcc tttaagtcct agattatcta aaaataagca tatgagggat
     5521 atgctactta ctatgcaaaa agattcagcg tattacgaaa caagtgacag tctagtcctt
     5581 cgaattaatc tcactgcaga tactaaactt aattttaatg ctgttaaagg agcgagtgct
     5641 cttactgaaa atatgatgat gagacagttt gcagttgctg gaccacaaga tgatcctgtt
     5701 agtgaacata aatacccatc agtatttctc ttaactcctg ccttattgga aactgctagt
     5761 gaggcaactc taaatggtaa ggaaatcaca gcatctggta ttatcggtca catcaaggat
     5821 ggtgataaaa gcaagcatgt tgaagtcaaa atggtgaatg aaaatggaga catgctagga
     5881 acccctgtta ttattcaagg taaagacttg actaatcgaa caaaaccatt aatgagtgga
     5941 cgtagagtac tttatgccgg taaacaatat gagttccggg ctaaattacc acttagtcgt
     6001 tttaacactt ggattagggt tgaagtggta acagaagcag gagagaaagc aagtattgtt
     6061 cgtcgcatgt tctttgacca atcagttcca gagcttaaca cagcagttgc taaacgtgat
     6121 ttgacttctg atactgctct tatccacatc gttgccaaag atgactctct aaaactaaaa
     6181 ttatatcaag atgattcatt acttgaatct gttgataaaa ccggtcttta tagttttaga
     6241 aatggtgtag aaatcactaa agatatgaca gtaccactag aatttggaga taatattatt
     6301 aagttatctg ctgttgactt atcaaattat cgtcgtaatg agacccttca tatctataga
     6361 aaccgttttg atgttaaagc aagccaaatg acagctgaca aaggagctaa agtaactgtg
     6421 gatatgttga tgaagcactt agttgttcca gaaatggcag gagcttatac attaacaatc
     6481 gacgaagctc caaacacaaa tgaatcagga atgttaacaa acgctaaagt atcgattcat
     6541 tatgtaaatg gtggtgttga taaagttgat gttccgatta aagtagttga cttagaagct
     6601 attcgtaaag ctgaagaagc acataaagct gacgaagcac gtaaagctga agaagcacgt
     6661 aaagctgaag aagcacataa agctgaagaa gtacgtaaag ctgaagaagc acataaagtc
     6721 gaagaagcac gtaaagctga agagggacat aaaacccaag aagcacctat agttgaagaa
     6781 ggctacaaag ttaataacgt tcatcaaact gatactacag ttaaagcgtc tgatttacca
     6841 aagactaaga cagtttccgc agttcatatg gctagaacag acaataaaca gataacttca
     6901 catcagacac atgttgaaaa acaaattaaa aatacattgc catccactgg tgacagcaaa
     6961 cgtggttatt atatcactgg aatggctatc gttatgctga gtgtattatt tagtttagct
     7021 aaaaagttta aaagcaaata ttaatgataa aataaaatct atttagcaac attaattgtg
     7081 atggataaaa ggttttatct cttaaatcaa aagtagtagc tcattagcta ctactttttt
     7141 gtataagtgt aaaaaagtga attcttaaca agaatttaac accctacttg ccatactaaa
     7201 agtgaataag aaagagaggt gtcatttatg caaagaaaat cctatttaaa atccatgagt
     7261 gttcttactt taacagcttg tcttatatca ggatatgtgg ttaaagatat tgctatgtta
     7321 catgcagtat ctgccagtga gaagaaagca aataatgtca gtccgagaga aaatctctac
     7381 agggctgtca atgataattg gctagccaat acaaaactca aacaagggca gactagtgtt
     7441 aatagttttt cagaaattga ggataaatta aagcaactgt tagtgtctga tatggctaaa
     7501 atggcctcag gaaagattga aacaaccaat gatgaacaga aaaaaatggt tgcatactat
     7561 aaacaaggta tggactttaa aacaagagat aaaaatggtc tcaaacctct aaaaccagtt
     7621 ttacaaaaac ttgaagcagt ctcttcaatg aaagactttc aaagtttggc ccatgatttt
     7681 gtgatgagtg gttttgtttt accatttggt ttgactgtgg aaaccaatgc tcgagataat
     7741 agccaaaagc aattggtgct tcgtcaagca cccgcattac ttgaatcacc tgaccaatat
     7801 aagaagggca ataaagaagg tgaggctaaa ttatcagctt accgtacttc agcaatggct
     7861 ttgcttaaac aagctggaaa aagtaacatt gaagatagaa aactagttaa acaagctata
     7921 gcatttgata gactcttatc agaaaaaacg caagttgatc aaagtaaaat cacagctgaa
     7981 agtgagacag ctgcggggcg atataaccct gaaagtatgg aaacggttca caattacgcc
     8041 aaggaatttg actttaaaga attgattgaa aaactagttg ggccaacgaa taaggcagtc
     8101 aatgtagaag ataaaactta ttttaaacag gttaatgatg ttataaatag taaacaatta
     8161 gccaatatga aagcatggat gatgatttct atgctagttg atcaatcaga ttttctagga
     8221 gaacaaaatc gtcaagcagc gagtgctttt aagaatgttg cgtctggttt gactcagatt
     8281 gaatcgaaag aaaaaatgct tacacccaat tagaatatac ttttggccat attttgggtg
     8341 attattatgg taagaaatat tttggtgagg cagctaaaaa agacgtcgaa catatggcta
     8401 agaaaatcat taatgtctat aaaacacggt taaaaaacaa cacttggtta tcagaaaata
     8461 caaaagcaat ggccattaag aaacttgata acatgagatt aatgattggc tatccagaag
     8521 attatcctga tctttatcgt cagtaccaat ttgatagtaa agcaagcttc tttgaaaaca
     8581 atgataacta cagaaaatta tcgaacaaga aaacatttga agaatttaac cagtctaatc
     8641 aacgtgaaca ttggcaaatg agtgccaatg ctgtaaatgc ttataatgat cctaatacca
     8701 attccatagt ctttccagca gcgatttttc aatcaccact ctacgataaa actaaaacag
     8761 ttagtcaaaa tcatggagct atcggagcaa ttattggtca tgaaatttca cactcatttg
     8821 atattaatgg tatgaaatat gacgagaaag ggaatcttca cgattggtgg actaaagaag
     8881 atttaaaaca ttataagaaa aaaacacaag ctatgattga ccaatgggat ggccttaaag
     8941 cagatggcgg taaagttgat ggtaaattaa ctttagcaga aaatattgca gataatggtg
     9001 gtgttatggc atctctagaa gctcttaaga ctgaaaaaat ccaaactata aagaattttt
     9061 tgaatcatgg gcaagtattt ggcgtcaaaa agcaaccaaa gaacaaagta agtcctcaat
     9121 tcagtcagat gttcatgcac catatgaatt gagagctaac atcccagtac gtaatttcca
     9181 agaattttat gatgcctttg gtgttaaaaa aggcgattca atgtatctaa aaccagaaaa
     9241 acgtttgaca ctttggtaaa gctatgtttt ccagacaata aggagaatgc atgaagaata
     9301 taacaaagct atcaactgtt gctttaagcc tactactttg tacggcgtgt gctgcatcaa
     9361 acacgtctac atctaaaaca cagtctcatc atcctaaaca aactaaactc acagataagc
     9421 aaaaagaaga acccaaaaac aaagaagctg ctgatcaaga gatgcatccc caaggcgctg
     9481 ttgatttgac aaaatataag gcaaaaccgg tcaaagatta tggtaaaaaa atcgatgttg
     9541 gtgatggcaa gaaaatgaac atttatgaaa ctggtcaggg aaaaattcca attgttttta
     9601 ttcctggtca agctgagatt tcgccacgct atgcttataa gaatttaatt gagcggttaa
     9661 gtaaaaaata taaaatttat acagtagaac cattaggcta tggtctcagt gatattccta
     9721 ct
//
LOCUS       DBJYSH010000075         9585 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.075, whole genome shotgun sequence.
ACCESSION   DBJYSH010000075 DBJYSH010000000
VERSION     DBJYSH010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 9585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9585
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.075"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            77..4189
                     /gene="cas9"
                     /locus_tag="ACUBV3_001445"
                     /gene_synonym="csn1"
     CDS             77..4189
                     /gene="cas9"
                     /locus_tag="ACUBV3_001445"
                     /gene_synonym="csn1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01865.2"
                     /note="Cas9, originally named Csn1, is the large,
                     multifunctional signature protein of type II CRISPR/Cas
                     systems. It is well known even to general audiences
                     because its RNA-guided endonuclease activity has made it a
                     popular tool for custom editing of eukaryotic genomes;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR RNA-guided endonuclease Cas9"
                     /protein_id="HHU3847943.1"
                     /translation="MNKPYSIGLDIGTNSVGYSVVTDDYKVPAKKMRVLGNTDKEYIK
                     KNLIGALLFDGGNTASDRRLKRTARRRYTRRRNRILYLQEIFAEEMSKVDDSFFHRLE
                     DSFLVEDDKRGSKYPIFATMQEEKDYHEKFPTIYHLRKELADKKEKADLRLVYLALAH
                     IIKFRGHFLIEDDSFDVRNTDIQRQYQAFLEIFDTTFENNHLLSQNIDVEGILTDKIS
                     KSAKKDRILAQYPNQKSTGIFAEFLKLIVGNQADFKKHFNLEDKTPLQFAKDSYDEDL
                     ENLLGQIGDEFADLFSVAKKLYDSVLLSGILTVTDLSTKAPLSASMIQRYDEHREDLK
                     QLKQFVKASLPEKYQEIFTDSSKDGYAGYIEGKTNQGAFYKYLSKLLTKQEGSEYFLE
                     KIKNEDFLRKQRTFDNGSIPHQVHLTELKAIIRRQSEYYPFLKENLDRIEKILTFRIP
                     YYVGPLAREKSDFAWMTRKTDDSIRPWNFEELVDKEASAEAFIHRMTNNDLYLPEEKV
                     LPKHSLIYEKFTVYNELTKVRYKNEQGETYFFDSNIKQEIFDGVFKEHRKVSKKKLLD
                     FLAKEYEEFRIVDVIGLDKENKAFNASLGTYHDLKKILDKDFLDNPDNESILEDIVQT
                     LTLFEDREMIKKRLENYKDLFTESQLKKLYRRHYTGWGRLSAKLINGIRDKESQKTIL
                     DYLISDGRANRNFMQLIHDDGLSFKPIIDKAQAGSHSDNLKEVVGELAGSPAIKKGIL
                     QSLKIVDELVKVMGYEPEQVVVEMARENQTTNQGRRNTRQRYKLLEEGVKNLASDLNG
                     NILKEYPTDNQALQNERLFLYYLQNGRDMYTGETLDIDNLSQYDIDHIVPQAFIKDDS
                     IDNRVLVSSAKNRGKSDNVPSIDIVKARKAFWKKLLDAKLMSQRKYDNLTKAERGGLT
                     SDDKARFIQRQLVETRQITKHVARILDERFNNELDSKGRRIRKVKIVTLKSNLVSNFR
                     KEFGFYKIREVNDYHHAHDAYLNAVVAKAILTKYPQLEREFVYGDYPKYNSYKTRKSA
                     TEKLFFYSNIMNFFKTKVTLADGTVVVKDDIEVNNDTGEIVWDKKKHFATVRKVLSYP
                     QVNIVKKTEIQTGGFSKESILAHGNSDKLIPRKTKDIYLDPKKYGGFDSPIVAYSVLV
                     VADIKKGKVQKLKTVTELLGITIMERFRFEKNPSAFLESKGYLNIRDDKLMILPKYSL
                     FELENGRRRLLASAGELQKGNELALPTQYMKFLYLASRYNELKGKPEEIEQKQEFVVQ
                     HVSYFDDILQIINDFSNRVILADANLEKINKLYQDNKENISVDELANNIINLFTFTSL
                     GAPAAFKFFDKIVDRKRYTSTKEVLNSTLIHQSITGLYETRIDLGKLGED"
     gene            4191..5061
                     /gene="cas1"
                     /locus_tag="ACUBV3_001446"
                     /pseudo
     CDS             4191..5061
                     /gene="cas1"
                     /locus_tag="ACUBV3_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000929484.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR-associated endonuclease Cas1"
     gene            5058..5399
                     /gene="cas2"
                     /locus_tag="ACUBV3_001447"
     CDS             5058..5399
                     /gene="cas2"
                     /locus_tag="ACUBV3_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HHU3847944.1"
                     /translation="MSYRYMRMILMFDMPTETVEERKAYRKFRKFLLSEGFIMHQFSV
                     YSKLLLNNTANNAMIGRLKVNNPKKGNITLLTVTEKQFARMVYLHGERNTSVANFDSR
                     LVFLGDSYDQD"
     gene            5386..6051
                     /gene="csn2"
                     /locus_tag="ACUBV3_001448"
     CDS             5386..6051
                     /gene="csn2"
                     /locus_tag="ACUBV3_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II-A CRISPR-associated protein Csn2"
                     /protein_id="HHU3847945.1"
                     /translation="MIKINFPILDEPLVLSNATILTIEDVSVYSSLVKHFYQYDVDEH
                     LKLFDDKQKSLKATELMMVTDILGYDVNSAPILKLIHGDLENQFNEKPEVKSMVEKLA
                     ATITELIAFECLENELDLEYDEITILELIKALGVKIETQSDTIFEKCFEIIQVYHYLT
                     KKNLLVFVNSGAYLTKDEVIKLCEYINLMQKSVLFLEPRRLYDLPQYVIDKDYFLIGE
                     NMV"
     repeat_region   6152..6517
                     /inference="COORDINATES: alignment:CRISPRCasFinder:4.3.2"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=6152..6187
                     /rpt_unit_seq="gttttagagctgtgctgtttcgaatggttccaaaac"
     gene            6570..6830
                     /locus_tag="ACUBV3_001449"
     CDS             6570..6830
                     /locus_tag="ACUBV3_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3847946.1"
                     /translation="MLGHRCGVLVFCYTEINFQRMWGKAVIRLIQDVIQNLVGQANEI
                     TPVYQFDWETYILATKKYERHLEVCLLVENSNYFSDSKRMCR"
     gene            6862..6999
                     /locus_tag="ACUBV3_001450"
     CDS             6862..6999
                     /locus_tag="ACUBV3_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3847947.1"
                     /translation="MVKGLDCSTLYLIISLTALVLLVIAGVSLSLILASLQGKQWRTY
                     P"
     gene            7140..7556
                     /gene="ndk"
                     /locus_tag="ACUBV3_001451"
     CDS             7140..7556
                     /gene="ndk"
                     /locus_tag="ACUBV3_001451"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HHU3847948.1"
                     /translation="MEQTFFMIKPDGVKRGFIGEVISRIERRGFSIDRLEVRHADADI
                     LKRHYAELTDRPFFPTLVDYMTSGPVIIGVISGEEVISTWRTMMGSTNPKDALPGTIR
                     GDFAQAPSPNQATCNIVHGSDSPESAAREIAIWFNN"
     gene            7692..9524
                     /gene="lepA"
                     /locus_tag="ACUBV3_001452"
     CDS             7692..9524
                     /gene="lepA"
                     /locus_tag="ACUBV3_001452"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HHU3847949.1"
                     /translation="MNIEDLKKRQEKIRNFSIIAHIDHGKSTLADRILEKTETVSSRE
                     MQAQLLDSMDLERERGITIKLNAIELNYTAKDGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRAEVEDVI
                     GLDASEAVLASAKAGIGIEEILEQIVEKVPAPTGEVDAPLQALIFDSVYDAYRGVILQ
                     VRIVNGMVKPGDKIQMMSNGKTFDVTEVGIFTPKAVGRDFLATGDVGYIAASIKTVAD
                     TRVGDTITLANNPAIDPLHGYKQMNPMVFAGLYPIESNKYNDLREALEKLQLNDASLQ
                     FEPETSQALGFGFRCGFLGLLHMDVIQERLEREFNIDLIMTAPSVVYHVNTTDGEMLE
                     VSNPSEFPDPTRVDSIEEPYVKAQIMVPQEFVGAVMELAQRKRGDFVTMDYIDDNRVN
                     VIYQIPLAEIVFDFFDKLKSSTRGYASFDYEISEYRRSQLVKMDILLNGDKVDALSFI
                     VHKEFAYERGKLIVDKLKKIIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKAGKKRMKAIGSVEVPQEAFLSVLSMDDDDKK"
BASE COUNT         3214 a         1444 c         1925 g         3002 t
ORIGIN      
        1 aataatcaag taaaaattag caaaacttta aagtttgcgt ttttaaagtg atattttaaa
       61 taaaaggaga taaattatga acaaaccata ttcaataggt ctcgacatcg gaactaattc
      121 cgtcggatat tctgtggtta cagatgatta taaggtacct gctaagaaga tgagggtctt
      181 agggaataca gataaagaat acataaagaa gaatcttata ggtgctctgc tttttgatgg
      241 tggaaatact gcttcagata gacgcttgaa gcgaactgct cgccgtcgtt atacacgtcg
      301 tagaaatcgt attctatatt tacaagaaat ttttgcagag gaaatgagta aagttgatga
      361 tagtttcttt catcgattag aggattcttt tctagttgag gacgacaaga gagggagcaa
      421 gtatcctatc tttgcaacaa tgcaggagga gaaagattat catgaaaagt tcccaacgat
      481 ttatcacttg agaaaagaat tagctgacaa gaaagaaaaa gcagaccttc gtcttgttta
      541 tctggctcta gctcatatca ttaaattcag aggacatttc ctaattgagg atgatagctt
      601 tgatgtgagg aatactgata ttcaaagaca atatcaagct tttttagaaa tctttgatac
      661 taccttcgaa aataaccatt tgttatctca aaacatagat gtagagggaa ttctaacaga
      721 taagattagc aagtctgcga agaaagatcg cattttagcg cagtatccta accaaaaatc
      781 tactggcatt tttgcagaat ttttgaaatt gattgtcgga aatcaagctg acttcaagaa
      841 acatttcaat ttggaagata aaacgccgct tcaatttgct aaggatagct acgatgaaga
      901 tttagaaaat cttcttgggc agattggtga tgaatttgct gacttattct cagtagcgaa
      961 aaagctatat gatagtgttc ttttatctgg cattcttaca gtaactgatc tcagtaccaa
     1021 ggcgccactt tctgcctcta tgattcagcg ttacgatgaa catagagagg acttgaaaca
     1081 gttaaaacaa ttcgtaaaag cttcattgcc tgagaaatat caagaaatat ttactgattc
     1141 atcaaaagat ggctacgctg gttatattga aggcaaaact aatcaaggag cattttataa
     1201 atacttgtca aaattgttga ccaagcaaga aggtagcgag tattttcttg aaaaaatcaa
     1261 gaatgaagat tttttgagaa aacaaaggac ctttgataat ggctcaattc cacaccaagt
     1321 ccatttgaca gaattgaagg ctattattcg acgtcaatca gaatactatc cattcttgaa
     1381 agagaatcta gataggattg aaaaaatcct tacctttaga attccttatt atgtcgggcc
     1441 actagcacgt gagaagagtg attttgcatg gatgactcgc aaaacagatg acagtattcg
     1501 accttggaat tttgaagaat tggttgataa ggaagcatct gcggaagctt ttatccatcg
     1561 tatgaccaac aatgacctct atcttcctga agaaaaagtc ctaccaaaac atagtcttat
     1621 ttatgaaaaa tttacggtct ataatgagtt gactaaggtt agatataaaa atgagcaagg
     1681 tgagacttat ttttttgata gcaatattaa acaagaaatc tttgatggag tattcaagga
     1741 acatcgtaag gtatccaaga agaagttgct agattttctg gctaaagaat atgaggagtt
     1801 taggatagta gatgttattg gtctagataa agaaaataaa gctttcaacg cctcattggg
     1861 aacttaccac gatctcaaaa aaatactaga caaagatttt cttgataatc cagataatga
     1921 gtctattttg gaagatatcg tccaaactct aaccttattt gaagatagag aaatgattaa
     1981 gaagcgtctt gaaaactata aagatctttt tacagagtca caactaaaaa aactctatcg
     2041 ccgtcactat actggctggg gacgattgtc tgctaagtta attaatggca ttagagataa
     2101 agagagtcaa aaaacaatct tggactatct gatttctgat ggaagagcaa atcgcaactt
     2161 tatgcagttg atacatgatg atggtctatc atttaaacca attattgaca aggcacaggc
     2221 tggtagtcat tcagataatc taaaagaagt tgtaggtgag cttgcaggta gccctgctat
     2281 taaaaagggg attctacaaa gtttgaaaat tgttgatgag cttgttaaag tcatgggata
     2341 cgaacctgaa caagttgtgg ttgagatggc gcgtgagaat caaacaacaa atcaaggtcg
     2401 tcgtaacact cgacaacgct ataaacttct tgaggaaggc gttaagaatc tagctagtga
     2461 cttgaatggc aatattttga aagaatatcc tacggataat caagcgttgc aaaatgaaag
     2521 acttttcctt tattacttac aaaatggaag agatatgtac acaggggaaa cactagatat
     2581 tgacaattta agtcaatatg atattgatca cattgttcct caagctttca taaaagatga
     2641 ttctattgat aatcgtgttt tggtatcatc tgctaaaaac cgtggaaaat cagataatgt
     2701 tcctagcatt gatattgtaa aagcacgcaa agcattctgg aaaaaattac ttgatgctaa
     2761 gttaatgagt cagcgtaagt atgataattt gactaaggca gagcgcggag gcctaacttc
     2821 cgatgataag gcaagattta tccaacgtca gttggttgag acacgacaaa ttaccaagca
     2881 tgttgcccgt atcttggatg aacgctttaa taatgagctt gatagtaaag gtagaaggat
     2941 tcgcaaagtt aaaattgtaa ccttgaagtc aaatttggtt tcaaatttcc gaaaagaatt
     3001 tggattctat aaaattcgtg aagttaatga ttatcaccat gcacatgatg cctatcttaa
     3061 tgcagtagtt gctaaagcta ttctaaccaa atatcctcag ttagagcgag aatttgtcta
     3121 cggtgactat ccaaaatata acagttacaa aacgcgtaaa tccgctacag aaaagctatt
     3181 tttctattca aatattatga acttctttaa aactaaggta actttggcgg atggaaccgt
     3241 tgttgtaaaa gatgatattg aagttaataa tgatacgggt gaaattgttt gggataagaa
     3301 gaaacacttt gcgacagtta gaaaagtctt gtcataccct caggtcaata tcgtgaagaa
     3361 gacagagatt cagacaggtg gtttctctaa ggaatcaatc ttggcgcatg gtaactcaga
     3421 taagttgatt ccaagaaaaa cgaaggatat ttatttagat cctaagaaat atggaggttt
     3481 tgatagtccg atagtagctt actctgtttt agttgtagct gatatcaaaa aaggtaaagt
     3541 acaaaaacta aaaacagtta cggaactttt aggaattacc atcatggaga gattcagatt
     3601 tgagaaaaat ccatcagctt tccttgaatc aaaaggttat ttaaatatta gggacgataa
     3661 attaatgatt ttaccgaagt atagtctgtt cgaattagaa aatgggcgtc gtcgattact
     3721 tgctagtgct ggtgaattac aaaaaggtaa cgagctagcc ttaccaacac aatatatgaa
     3781 gttcttatac cttgcaagtc gttacaatga gctaaaagga aaaccagaag aaattgaaca
     3841 aaaacaggaa tttgtagttc aacatgtttc ttatttcgac gatattcttc aaataatcaa
     3901 cgatttttca aatcgagtta ttctagcaga tgctaattta gagaaaatca ataagcttta
     3961 ccaagataat aaggaaaata tatcagtaga tgaacttgct aataatatta tcaatctatt
     4021 tacttttacc agtctaggag ctccagcagc ttttaaattt tttgataaaa tagttgatag
     4081 aaaacgctat acatcaacta aagaagtact taattctacc ctaattcatc aatctattac
     4141 tggactttat gaaacacgta ttgatttggg taagttagga gaagattgat atggcaggtt
     4201 ggcgaaccgt tgttgtaaat acacattcta agctctctta taaaaataat catctggttt
     4261 ttaaagattc ttatcagacg gaaatgattc atctatcaga gattgacatt ctaatcatgg
     4321 aaacaacaga tatcgttttg tcgaccatgc tgattaaacg tttggttgat gaaaatattt
     4381 tagttatatt ttgtgatgac aaacgcttgc caacagctat gttaatgccg tactatgcca
     4441 gatatgattc gagtttacaa ttatctaggc agatgtcatg gattgaagat gtcaaagcgg
     4501 atgtttggac gtcaatcatt gcacaaaaaa ttttgaatca gtctttttat cttggtgagt
     4561 gttctttctt tgaaaaatcc cagtctatta tgaatctcta tcatgattta gaatcttttg
     4621 atacttccaa tcgtgaaggg catgcagcta ggatttattt caatacactt tttggaaatg
     4681 atttttcaag agaacatgat aatccaataa atgctggttt agactacgga tattcattgc
     4741 ttttgagtat gtttgcgcgt gaagtcgtta agtgtggttg catgacacaa tttgggttaa
     4801 agcatgctaa tcaatttaat cagttcaacc tagcaagcga tattatggaa ccatttcgcc
     4861 caatcgttga taggattatt tatgaaaata ggcagagtga ttttgtcaaa atgaaaagag
     4921 aactcttttc tatgttttca gagacataca gttacgacgg taaagaaatg tatctttcaa
     4981 atattgtcag cgattacacc aaaaaaagtt attaagtcgc taaatagtga tgggaatggg
     5041 attccggagt ttaggatatg agttatcggt atatgagaat gattttaatg tttgatatgc
     5101 ctactgaaac agtagaagaa cgtaaggcgt atcgtaagtt tagaaagttt cttttgagcg
     5161 aaggttttat tatgcatcag ttctctgttt atagtaaatt attactcaat aatacagcta
     5221 ataatgctat gataggtcgg cttaaagtga ataatcctaa gaagggtaat atcacactct
     5281 taacagttac ggaaaaacaa tttgcgagaa tggtttacct ccatggagaa cgcaacacaa
     5341 gtgttgccaa ctttgatagt cgcttggttt tcctaggaga ttcttatgat caagattaat
     5401 tttccaattt tagatgaacc attagtgtta agtaatgcta cgattttaac gatagaagat
     5461 gtttcagttt attcttcatt ggtgaaacat ttttatcaat atgacgtaga tgaacatttg
     5521 aaattatttg atgataagca gaaaagtctg aaggcaacag agttaatgat ggttacagat
     5581 atcttaggat acgatgtcaa ctcagcacct attctaaagt tgatacatgg tgacttagaa
     5641 aatcaattca acgaaaagcc agaagtgaaa tcaatggtag aaaaattagc agctactatt
     5701 acagaactta tcgcatttga gtgtctagag aatgagcttg atttagaata cgatgaaatc
     5761 acgattttag aactcattaa ggcactagga gtcaaaattg agacacagag cgacactatc
     5821 tttgaaaaat gttttgaaat tatacaagtt taccattatt taacgaaaaa gaatctcttg
     5881 gtttttgtta atagcggagc ttatcttacc aaagatgaag ttataaaatt atgtgaatac
     5941 atcaatttaa tgcaaaagtc agtactcttt ctagaaccta gaagactcta tgatttaccg
     6001 caatatgtta ttgataagga ttatttcttg ataggcgaaa atatggtata atattagtaa
     6061 aagcacagta ataacaagga atcatcgaaa ctgaagtcct gctgagacga atggcgcgat
     6121 tacgaaagct caaaagaaaa ttttctacga ggttttagag ctgtgctgtt tcgaatggtt
     6181 ccaaaacatg aagaaatggc gaaaagaggt ttctggagtt ttagagctgt gctgtttcga
     6241 atggttccaa aactaagtat tgtccgtata ccataacatc atggttttag agctgtgctg
     6301 tttcgaatgg ttccaaaact atttgatagc ggtaacgggt catatacaag ttttagagct
     6361 gtgctgtttc gaatggttcc aaaactggtg gtatttataa tgtacgagca aatcggtttt
     6421 agagctgtgc tgtttcgaat ggttccaaaa cgataaaaag tgggagctga attaaaaggc
     6481 agttttagag ctgtgctgtt tcgaatggtt ccaaaacatt tgaacgattt ttatattcct
     6541 gatatgtgtt ttagagctgt gcggttatta tgctagggca ccgttgtggt gttctagttt
     6601 tttgttatac tgaaataaat tttcagagaa tgtgggggaa ggcggtaatt agattaattc
     6661 aagacgtaat tcagaactta gttggccaag ctaacgaaat caccccagtt tatcagtttg
     6721 attgggaaac ttatatattg gcgactaaaa aatatgaacg tcatttagag gtgtgtctat
     6781 tagtagaaaa ttcgaattat ttttcggatt caaagagaat gtgtcgatag aagagatacg
     6841 aaagactata attccaacct tatggttaaa gggctagatt gttctacgct ttacttaatt
     6901 attagtttga cagcgttggt tcttttagtg attgctggtg tttctttgtc actaatactt
     6961 gcaagcttac aaggaaagca gtggagaacc tatccataat caactaacag ctatgatgga
     7021 tctcacctat taatatagtt aaggagtgtc cgacactcct tttttgaaat acagttggaa
     7081 gttacagaaa acatattttc atgttataat agtggagaaa gaaatgaggt gctaatatta
     7141 tggaacaaac cttttttatg ataaaacctg atggtgtaaa acgtggtttt attggagaag
     7201 ttattagtcg tattgagcgt cgaggcttct ctattgatcg cttagaggtt agacatgcag
     7261 atgcagatat tttgaaaaga cattatgcag aactgacgga tagaccattc tttccaacat
     7321 tagtagatta tatgactagt ggtccagtaa tcattggagt tatttcggga gaagaggtca
     7381 tttcaacttg gcgcaccatg atggggagta ctaaccccaa agatgccctt ccaggaacta
     7441 ttcgaggaga ttttgcacaa gctccctctc caaaccaagc gacatgtaat attgttcatg
     7501 ggtcagactc acctgaatct gcagctcgcg agatagccat ttggttcaat aactaataga
     7561 cttaatgaag attgatgttc aagtgttaat tctcttttac atcggtcttc tttttgatat
     7621 aatattctag agtctatagg actgttttaa tattttagaa gttaaacata tcaaattgta
     7681 taggaaatag aatgaatatt gaagatttga agaaacgtca agaaaagatt agaaactttt
     7741 cgattatcgc tcatattgac catggaaaat caaccttagc agaccgtatt ttagagaaaa
     7801 cagaaacggt ttctagtcgt gaaatgcagg cgcaactgtt ggactctatg gacctagaac
     7861 gtgagcgtgg gattactata aagttaaatg ctattgagct taattatact gctaaagatg
     7921 gtgaaactta catttttcac ttgattgaca cgccagggca tgttgacttt acatatgaag
     7981 tatcacgttc attggcagct tgtgagggtg ctatactggt tgttgatgca gcccaaggaa
     8041 ttgaagctca aactctggca aatgtttatt tggctctaga caatgattta gagatactac
     8101 cagttattaa taaaattgat ttaccagcag cagatccaga acgtgtacgt gcagaagtag
     8161 aggatgttat tggtctagat gcttcagaag ctgtacttgc ctcagctaag gcagggattg
     8221 gtattgaaga aattttagag cagatcgttg aaaaagttcc tgctccgact ggggaagtag
     8281 atgctcctct tcaagctcta atttttgatt ctgtttacga tgcttatcgt ggagtaattc
     8341 tgcaagtaag gattgtaaat ggtatggtta agccaggaga taagattcaa atgatgtcta
     8401 atggtaaaac cttcgacgtt actgaggttg gtattttcac tcccaaagca gttggacgtg
     8461 attttttagc aactggagat gtagggtata ttgctgcctc aattaaaaca gttgctgata
     8521 cacgtgttgg tgataccatt acccttgcta ataacccagc tatagacccc ttgcatggct
     8581 ataagcaaat gaacccaatg gtttttgccg gtctttatcc aattgaatca aataagtata
     8641 acgatctgcg tgaagccctt gaaaaattac aattgaatga tgctagtcta cagtttgaac
     8701 cggaaacatc acaagcatta gggtttggtt tccgttgtgg tttcttagga cttttacata
     8761 tggacgttat ccaagagcgc ttggaacgag agttcaatat tgatcttatt atgactgcac
     8821 catcagtagt ttatcatgtt aatacaactg atggtgaaat gcttgaagtt tcgaatccat
     8881 ctgaatttcc tgatcctact cgtgttgaca gcattgaaga accatatgtt aaggcgcaaa
     8941 ttatggttcc tcaagaattt gtcggtgctg taatggagtt agcacaacgt aaacgtggtg
     9001 actttgtaac aatggattat attgatgata atcgtgttaa tgttatttac caaattcctt
     9061 tagctgaaat tgtgtttgac ttctttgata agcttaaatc atcaacacgt ggctatgcta
     9121 gttttgatta tgaaatttcc gaatatcgtc gctcacagtt agttaaaatg gatattcttt
     9181 taaatggtga taaagtggat gctttaagct ttattgttca caaagaattt gcctatgaac
     9241 gtggtaaatt gatcgttgat aagttgaaaa aaatcattcc tcgtcagcag tttgaagttc
     9301 ctatccaagc agctattgga caaaaaattg tggctcgttc tgatataaaa gcactacgca
     9361 aaaatgtctt agcaaaatgt tatggtggtg acgtttcacg taaacgtaag ttgcttgaaa
     9421 aacaaaaagc tggtaaaaag cgtatgaaag caattggttc cgtcgaagtt cctcaggaag
     9481 ctttcttaag tgttttatca atggacgatg atgacaaaaa ataattcaga aagatgatat
     9541 catttatcta ttttaaagta acttagcttc gtgcttaagt tactt
//
LOCUS       DBJYSH010000076         9556 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.076, whole genome shotgun sequence.
ACCESSION   DBJYSH010000076 DBJYSH010000000
VERSION     DBJYSH010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 9556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9556
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.076"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            60..1268
                     /gene="sstT"
                     /locus_tag="ACUBV3_001453"
     CDS             60..1268
                     /gene="sstT"
                     /locus_tag="ACUBV3_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HHU3847950.1"
                     /translation="MKRFLKAWRKTSLIKKITIGVVIGLFLGILVPKASAIGLLGQLF
                     VGGLKAIAPLLVFTLVISALSQHREGGKTNMSTIIGLYITATFAAALIAVVVNYIFPL
                     TLILKTPAKTDLLPPKGISEVFQSLLLKIVDNPIHAITEANYMSILFWAVIFGLAMRS
                     SNQRTKDLMQTFADATSQVVKWIINLAPIGIMGLVFTSISENGIAILGDYGLLILVLV
                     GTMLFVALVVNPIIAFVMMRKNPYPLVLRCLKDSGITAFFTRSSAANIPVNMRLCEDL
                     GLDKDTYSVSIPLGAAINMAGAAITINILTLAAVNTLGITVDFPTAFLLSVVAAVSAC
                     GASGVTGGSLLLIPVACSLFGISNDVAMQVVGVGFIVGVIQDSCETALNSSTDVLFTA
                     VAEKSVFGKK"
     gene            1437..1985
                     /locus_tag="ACUBV3_001454"
     CDS             1437..1985
                     /locus_tag="ACUBV3_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type riboflavin transporter
                     substrate-binding protein"
                     /protein_id="HHU3847951.1"
                     /translation="MNTNTIKKVVATGIGAALFIIIGMLVNIPTPIPNTNIQLQYAVL
                     ALFAVIYGPGVGFFTGFIGHALKDSIQYGSPWWTWVLVSGLLGLMIGFFAKKLAIQLS
                     GMTKKDLLLFNVVQVIANLIGWSVVAPYGDIFFYSEPASKVFAQGFLSSLVNSITIGV
                     GGTLLLLAYAKSRPQKGSLSKD"
     gene            2002..3678
                     /locus_tag="ACUBV3_001455"
     CDS             2002..3678
                     /locus_tag="ACUBV3_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HHU3847952.1"
                     /translation="MKDFIEWKDFTFQYDVQSEPTLKGINLSIPKGEKVLILGPSGSG
                     KSTLGHCLNGIIPNTHKGQYSGIFTINHKNAFDLSIYDKSHLVSTVLQDPDGQFIGLT
                     VVEDIAFALENDVVAQEEMASIVEMWAKRLEIAPLLSKRPQDLSGGQKQRVSLAGVLV
                     DDSPILLFDEPLANLDPQSGQDIMALVDRIHQEQDATTIIIEHRLEDVLYERVDRVVL
                     FSDGQIIYNGEPDQLLKTNFLSEYGIREPLYISALKNLGYDFEKQNTMTSIDDFDFSE
                     LLIPKMRALDLDKHTDKLLSVQHLSVSYDLENNTLDDVSFDLYKGQRLAIVGKNGAGK
                     STLAKALCQFIPNNATLIYNNEDVSQDSIKERAERIGYVLQNPNQMISQAMVFDEVAL
                     GLRLRGFSDNDIESRVYDILKVCGLYQFRNWPISALSFGQKKRVTIASILILNPEVII
                     LDEPTAGQDMKHYTEMMSFLDKLSCDGHSIVMITHDMQLMLEYTDRAIVIDNGLIAAD
                     DHPINILSNTELLKKTHLKKTSLFALADRLGISPQKLTQWYIDNQGGKNG"
     gene            3671..4501
                     /locus_tag="ACUBV3_001456"
     CDS             3671..4501
                     /locus_tag="ACUBV3_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T family protein"
                     /protein_id="HHU3847953.1"
                     /translation="MANQLIGYQEGYGFLYRLSGASKLLFFILVSVACMTTYDTRFLI
                     LVSLGSLVLFRLAKIKWQQVSFVMTLVVVFAVLNIIMVYLFAPHYGDKIYGSSSLLLK
                     GIGPYDVTSQELFYLFNLILKYFCTVPLALLFLMTTNPSQFASSLNQLGLSYKIAYAV
                     SLTLRYIPDVQEEFYTIRRAQEARGIELSKKSNLVARIKGNLQIVTPLIFSSLERIDT
                     VATAMELRRFGKNKRRTWYSKQSLEKSDIVLIILALASLFVSLYLIHLNHGRFYNPWQ
                     "
     gene            complement(4536..5207)
                     /locus_tag="ACUBV3_001457"
     CDS             complement(4536..5207)
                     /locus_tag="ACUBV3_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HHU3847954.1"
                     /translation="MKTKIIGVLGLGIFGQTLAQELSNFEQDVIAIDSNPENVQAVAE
                     VVTKAAIGDITDLAFLKHIGISDCDTVIIATGNSLESSVLAVMHCKKLGVPQVIAKAR
                     NLVYEEVLYEIGADLVISPERESGQNVAANLMRNKITDVFQIESDISVIEFKIPKSWV
                     GKTVEQLNIRHKFDLNLIGIRKAKNKPVDTEVPINSPLEEGIILVAIANSDAFQRYDY
                     LGYFN"
     gene            complement(5220..6599)
                     /locus_tag="ACUBV3_001458"
     CDS             complement(5220..6599)
                     /locus_tag="ACUBV3_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HHU3847955.1"
                     /translation="MKHFFDYKTMSVARKLSISFIAVILLGSILLSLPIFQYANAPKT
                     HYIDHLFTTVSMVCVTGLSVFPISKVYNGWGQIVAILLMQTGGLGLVTLMSLSYYTLR
                     RKMSLNDQTLLQSAITYNSSTDLKKYLYMIFKVTLTLEVLAASILAIDFIPRFGLGHG
                     IFNSIFLAVSAFCNAGFDNLGATSLAQFKLNPLVNIIVCFLIISGGLGFAVWKDLIEA
                     TIQTSHKGPKLIKTFPKRLSNHSKLVLKTTTIILLTGTLLSWLLEFGNFRTIANLSLP
                     KQLMVSFFQTVTMRTAGFSTIDYTQTDFATNLVYIIQMLIGGAPGGTAGGFKVTVIAI
                     LLLLFKAELSGQSQVTFHYRTIPSSIIKQTLSILTFFFIILISGYLLLLELNPHIDPF
                     SLFFEASSALATVGVTMNTTNQLSLGGRIVIMFLMFIGRVGPITVLLSILQKKEKEIH
                     YAETEIILG"
     gene            complement(6614..7327)
                     /gene="rsmG"
                     /locus_tag="ACUBV3_001459"
     CDS             complement(6614..7327)
                     /gene="rsmG"
                     /locus_tag="ACUBV3_001459"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HHU3847956.1"
                     /translation="MTPQAFYQVLIEHGITLTDKQKKQFETYFRLLVEWNEKINLTAI
                     TDKEEVYLKHFYDSIAPILQGYIDNSPLSILDIGAGAGFPSIPMKILYPEIDITIIDS
                     LNKRINFLNILANELELSGVHFFHGRAEDFGQDKVFRAKFDIVTARAVARMQVLAELT
                     IPFLKVNGRLIALKAAAAEEELISAEKALKTLFSQVTVNKNYKLPNGDDRNITIVSKK
                     KETPNKYPRKAGTPNKKPL"
     gene            7455..8009
                     /locus_tag="ACUBV3_001460"
     CDS             7455..8009
                     /locus_tag="ACUBV3_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000537058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HHU3847957.1"
                     /translation="MGTMILIAIIALFVIWLIVAYNSLVRSRMHTKESWSQIDVQLKR
                     RNDLIPNLIETVKGYAAYEGKTLEKIAELRAQVAKANTPAEAMTASNELTRQLSSILA
                     VAENYPDLKANNSFVKLQEELTNTENKISYSRQLYNTTTSNYNVKLETFPSNIVGKLF
                     GFKPSQFLETPEEEKEVPKVSFDF"
     gene            8030..8920
                     /gene="htpX"
                     /locus_tag="ACUBV3_001461"
     CDS             8030..8920
                     /gene="htpX"
                     /locus_tag="ACUBV3_001461"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000966825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HHU3847958.1"
                     /translation="MLYQQIASNKRKTVVLLIVFFCLLAAIGAAVGYLVLGSYQFGLV
                     LALIIGVIYAVSMIFQSTNVVMSMNNAREVTEDEAPNYFHIVEDMAMVAQIPMPRVFI
                     VEDDSLNAFATGSKPENAAVAATTGLLAVMNREELEGVIGHEVSHIRNYDIRISTIAV
                     ALASAVTLISSIGSRMLFYGGGRRRDDDREDGGNILVLIFSILSLILAPLAASLVQLA
                     ISRQREYLADASSVELTRNPQGMISALEKLDRSEPMGHPVDDASAALYINDPTKKEGL
                     KSLFYTHPPIADRIERLRHM"
     gene            9025..>9556
                     /locus_tag="ACUBV3_001462"
     CDS             9025..>9556
                     /locus_tag="ACUBV3_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000927541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HHU3847959.1"
                     /translation="MLLTEIKKSPEGLYFDKKIDIKESLMERHSEIMDISDIQVSGHV
                     VYEDGLYLLDYNMAYDITLPSSRSMKPVVLSEKQTINEVFIEAENVSTKKELVDQDLV
                     LILEEDDINLEESVIDNILLNIPLRVLAADEVGVEADLSGKNWSLMTEKQYEEKQAKE
                     KEKSNPFAALEGMFDSD"
BASE COUNT         3004 a         1524 c         1846 g         3182 t
ORIGIN      
        1 tttgacgaat cttttttttt atcatatcat agagtttaag aattttttag gagtttagta
       61 tgaaacgatt tttaaaagct tggagaaaaa caagcttgat aaaaaaaata acaattggag
      121 tcgttattgg cttattcttg ggtattttag tccctaaagc ctctgcgatt ggtttacttg
      181 gtcaactttt tgtaggaggg ttgaaagcta ttgcccctct tttagttttc actttagtta
      241 ttagtgcctt gtcgcaacac cgtgagggcg gtaagaccaa tatgtcaacg ataattggtt
      301 tgtatatcac tgcaactttt gcagcggccc taatcgctgt tgttgtgaac tatattttcc
      361 ctttgacact gatattgaaa acgccagcaa agacagactt attacctccc aaggggattt
      421 cagaagtctt tcaatctctt ttgttgaaaa ttgttgataa tcccattcat gccattaccg
      481 aggccaatta tatgagtatt ctattttggg cggttatttt tggtttggcg atgcgaagca
      541 gtaatcagcg tacaaaagat ttaatgcaaa cttttgctga tgctacttca caagttgtca
      601 aatggattat caatttggcc ccaattggta ttatggggtt ggttttcact agcatttcag
      661 aaaacggtat tgctatacta ggtgattatg gtcttttaat attagttttg gtgggtacga
      721 tgttatttgt agctcttgtc gttaacccaa tcattgcttt tgttatgatg cgtaaaaatc
      781 cttacccttt ggtattacga tgcttaaagg attcaggtat tacagctttc tttacacgta
      841 gttctgcggc gaatattcct gttaatatga gactatgtga ggatttagga ttagataaag
      901 acacttactc agtatctatc ccgcttggtg cagctatcaa tatggctggt gcagctatta
      961 caattaatat cctgacttta gcagcagtaa atactttagg aattactgtt gattttccaa
     1021 cagcattctt attaagtgtg gttgctgctg tatcagcatg tggagcttct ggtgtaactg
     1081 gtggttcact tttattgatt ccggtggcat gtagcttatt tggtatttca aatgacgttg
     1141 caatgcaagt cgtaggagtt ggtttcattg taggggtgat acaggattcg tgtgagacag
     1201 ccttaaattc atcgacagat gttctgttca cggctgtagc tgaaaaatca gtttttggaa
     1261 aaaaataaac taatgaaggt cttaggtatt ctatctgaga tcttttttgg tatttaatat
     1321 tattttcaat cgtttttcag ttcttcttgt caataaggtt tcaaatcagc ttgaaatatg
     1381 ataaaataaa acagattgta agtgactgtt taagcttgtt tttcagagag gtttttatga
     1441 atacaaacac aataaaaaag gttgtagcga ctggaattgg agctgcactt tttatcatta
     1501 taggtatgct agttaatata ccaacaccaa ttccaaatac taatattcaa ttgcagtatg
     1561 cggttttagc attatttgca gttatttatg gaccaggagt tggttttttt actggtttta
     1621 tcggacacgc attgaaagat tcgattcaat atggttctcc gtggtggact tgggttttag
     1681 ttagtggtct tttaggtttg atgataggct tttttgctaa aaaattagct attcaattat
     1741 ctggcatgac taagaaggat ttgcttctat ttaatgtagt tcaagttatt gctaacctta
     1801 taggttggtc tgtcgttgca ccgtatggtg atattttctt ttatagcgaa cctgcaagca
     1861 aagtttttgc acaaggtttc ttatcaagtc ttgtgaattc cattactatt ggagtaggtg
     1921 gtacgctttt gttgctagca tacgctaaat cacgaccaca aaaaggtagt ttatccaaag
     1981 attaggataa gagagtaatc tatgaaagat tttattgaat ggaaggattt tacctttcaa
     2041 tatgacgttc aatctgaacc aactttaaaa gggattaact taagcattcc taaaggtgaa
     2101 aaggtcttga tattaggacc ttcagggtcg gggaagtcaa cgcttggtca ttgtttaaat
     2161 ggtattatcc caaatactca taaaggccaa tatagtggta tttttacgat taaccataaa
     2221 aatgcttttg acttgtcaat atatgacaag tctcatttag tttctacggt tttacaagac
     2281 ccagatggcc aatttatagg attgacagta gtagaagata ttgcttttgc attagaaaat
     2341 gacgttgtag cacaggaaga aatggcctct atcgttgaaa tgtgggcaaa acgtttagaa
     2401 atagctcctc ttttgtctaa acgtccacaa gacttgtcgg gaggacaaaa acaacgggtt
     2461 agtttagctg gagttttagt agacgatagc ccaattctat tatttgatga gcctttggct
     2521 aatctagatc cacaatcggg acaagatatc atggcattgg ttgaccgtat tcatcaagag
     2581 caagatgcga ccactattat tattgaacat cgattggaag atgttcttta tgagcgagta
     2641 gatcgtgtag ttctttttag tgatggacaa attatttata atggggagcc ggatcaactt
     2701 ttaaaaacta attttttatc tgaatatggt attcgagaac cactgtatat ttctgccctc
     2761 aaaaatttag gatatgactt tgaaaaacaa aatacaatga cgtctattga tgactttgat
     2821 ttcagtgaac tattgatacc taaaatgcga gcattagatt tagataaaca tacagataaa
     2881 ttattatcag tacagcattt gtcggtgtca tatgatttag agaataatac tttagatgat
     2941 gtttcgtttg atttatataa aggtcagcgg ttagctatcg ttgggaaaaa tggtgcaggt
     3001 aaatctactt tagctaaagc gttatgtcaa tttatcccta ataatgctac tctaatttat
     3061 aataatgaag atgtcagtca agactctata aaagaaagag cagaacgaat tggctatgtt
     3121 ttgcaaaatc ccaatcagat gataagtcaa gcgatggttt ttgatgaagt tgctctgggt
     3181 ctccgtttaa gaggttttag tgataatgat atagaaagtc gtgtctacga tatattaaag
     3241 gtttgtgggc tctaccaatt taggaattgg cctatatcgg ctctatcatt cggtcagaaa
     3301 aaacgtgtta cgattgcttc gatactgatt ttaaatccag aagttattat tttggatgaa
     3361 ccaacagctg gtcaagatat gaagcattat actgaaatga tgtctttcct tgacaaacta
     3421 agttgtgatg gacatagcat tgttatgatt acacatgata tgcagcttat gttagagtat
     3481 acagatcgtg caattgtaat cgacaatggt cttattgctg cagatgatca tcctattaac
     3541 atcctttcaa atacagagct tttgaaaaag actcatttaa agaaaacgag tttatttgct
     3601 ttagcagaca gattagggat atctccacag aaactaacac aatggtacat tgataatcaa
     3661 ggaggtaaga atggctaatc aattaattgg ttatcaagag ggttatggct ttctatacag
     3721 actatcaggt gccagtaagt tactattttt tattttagtt tctgttgctt gcatgaccac
     3781 ttatgataca agatttttaa ttcttgtttc attgggatcg ctagtcttat ttagattagc
     3841 aaaaatcaaa tggcaacagg tgtcctttgt tatgacttta gttgttgtat tcgctgtttt
     3901 aaatattata atggtctatc tttttgctcc ccattatggt gataagattt atggtagttc
     3961 ttctttattg ttaaaaggaa ttggcccgta tgatgtgact agccaagagt tgttttacct
     4021 ctttaatctg attttaaaat acttttgtac agtgccttta gctcttttgt ttttgatgac
     4081 aactaatcct agtcagttcg catcaagttt aaatcaatta ggtttatcat ataaaatagc
     4141 ttatgctgtt agtttgaccc ttagatatat acctgatgtt caagaagaat tttatacaat
     4201 acgtcgagca caagaggcaa gaggaataga attatcaaaa aaatcgaact tagtagcacg
     4261 cattaagggg aatttacaga ttgtcacacc attaattttt agctctctag aacgtattga
     4321 tacagtagca actgctatgg aacttcgacg atttggtaaa aataaaagac gtacttggta
     4381 ctctaagcaa tctttggaaa aaagtgacat tgtcttaatc atacttgctc tagctagtct
     4441 gtttgtaagc ctttatttaa ttcaccttaa tcatggacgt ttctataacc catggcaata
     4501 aaaaactgac cttaatagtc agttttttat tttctttagt taaagtatcc taaatagtca
     4561 tagcgttgga aggcatctga gttagcgata gccactagaa taataccttc ttcaagtgga
     4621 ctattgattg gaacttcggt atcgacaggt ttgttttttg cttttctaat acctatgagg
     4681 ttaagatcaa acttgtgacg gatatttaat tgttcgacag ttttccctac ccatgatttg
     4741 ggaattttaa attcaatgac agaaatatca gattcaatct ggaagacatc tgtaatttta
     4801 tttctcatga ggtttgcagc aacattttgc ccagattctc gctccggaga gataaccaaa
     4861 tcagcaccaa tttcataaag tacttcttcg tatacaaggt ttcgagcttt agcaataact
     4921 tgagggacgc ctaacttttt acagtgcatt acggccaata ctgagctctc taaactgttt
     4981 cctgtagcaa taataacagt atcacagtca ctgatcccga tgtgttttag gaaagctaaa
     5041 tcagtaatgt ctccgatagc tgcttttgta acaacttcag cgacagcttg tacattttca
     5101 ggattgctgt caatagcaat aacatcttgt tcaaagttac ttagttcttg tgcgagtgtt
     5161 tgtccaaata ttcctagacc aagtacacca ataatttttg ttttcataaa agaccttttc
     5221 taacctagaa taatttctgt ttcagcatag tgaatttcct tctctttttt ctgaagaatt
     5281 gatagcagta cggtaattgg accgactcga ccaataaaca tgaggaacat aattacaatt
     5341 cgaccgccca gacttaattg attggtagtg ttcattgtca cacccacggt tgctaaggca
     5401 ctagatgcct caaagaaaag agaaaaagga tctatatggg gattgagttc cagaagaagg
     5461 agatagccgc taataagtat gataaaaaag aaagttaaaa tcgatagtgt ttgtttaata
     5521 atactggaag gaattgttcg ataatgaaaa gtcacttggc tttggccact cagttcggcc
     5581 ttaaatagta agagtaaaat ggcgataaca gtgactttaa atcctcctgc cgttccacca
     5641 ggagctcctc caattaacat ttggataata taaacaaggt tggttgcgaa atcagtttgg
     5701 gtataatcaa tagttgaaaa tccagcggta cgcatcgtaa ctgtttggaa aaagctgacc
     5761 attagttgtt taggtagaga taaattagca attgttctaa aatttccaaa ctctaacaac
     5821 caagaaagca aagttcctgt tagtaaaata atggttgttg tttttaaaac cagtttagaa
     5881 tggttgctga ggcgttttgg aaatgtcttg attaattttg gtcccttgtg agatgtttgt
     5941 atagtagctt caattaaatc tttccatacg gcaaatccaa gtcctcctga aataatcaag
     6001 aaacaaacga taatgttaac caaaggattt agtttaaatt gagctaagct cgttgcccct
     6061 agattatcaa aaccggcatt acaaaaggct gagacagcta agaatatact attaaagata
     6121 ccatgaccca aaccaaatcg aggaataaaa tctatggcta aaatgctggc tgctaaaact
     6181 tctaaagtta aagttacttt aaaaatcata tagagatatt tttttaaatc tgtcgaactg
     6241 ttataagtta tcgctgactg caaaagggtc tgatcattta acgacatttt acgtcttagg
     6301 gtatagtaag acaacgacat tagtgttact aaaccaagtc cccctgtttg catgagtaaa
     6361 atagctacaa tctgccccca accgttataa accttactga taggaaaaac agataaacca
     6421 gttacacata ccattgaaac agtcgtgaaa aggtgatcaa tgtaatgtgt tttaggggcg
     6481 ttagcatatt gaaaaattgg aagtgaaagt aaaatacttc ccaataggat aacagcgata
     6541 aaactaatgc tgagtttacg tgcgacagac attgtcttgt aatcaaagaa atgtttcata
     6601 ctagctccca actttacaaa ggcttcttgt ttggagtacc agctttacga gggtatttat
     6661 taggcgtttc ttttttcttg ctaacgattg tgatatttcg gtcatcacca ttaggtaact
     6721 tataattttt atttacagtt acttgtgaaa atagtgtttt caaagctttt tctgcagaaa
     6781 ttaattcctc ttcagcagct gcagctttca gtgcaatcaa gcgaccatta actttaagaa
     6841 aaggtattgt caattctgct agaacctgca ttctagctac tgcacgagct gttacgatat
     6901 caaatttagc tcgaaatact ttatcttgac caaagtcctc agcgcgccca tggaagaagt
     6961 gaactcctga aagttcaagt tcattagcta atatgtttag aaaattaatg cgtttattca
     7021 gtgaatcaat aatagtaata tcaatttcag gataaaggat tttcatagga atactaggaa
     7081 aacctgcacc agcaccaata tctaaaatgc taagaggact attgtcaata taaccttgta
     7141 aaatcggcgc aatagaatca tagaagtgct ttaagtaaac ctcttcctta tctgtaatag
     7201 ctgttaggtt aattttctca ttccattcaa caagaagtct aaaataagtt tcaaattgtt
     7261 ttttctgttt gtcagtaagt gtaataccat gctctatcag aacttgataa aatgcttggg
     7321 gtgtcatagt aaacctcaat ttctatatcg tataatgact ctattttatc acaaatacta
     7381 ataatgggaa taacaggact ttcctaatct aaagaaaatt gctataatgg aaaaaatgta
     7441 aaggagctac tactatggga actatgattt taatagcaat tattgctttg tttgttattt
     7501 ggttgattgt tgcttataac agtttagtta gaagtcgcat gcatactaaa gaatcatgga
     7561 gtcaaataga cgttcaattg aaacgtcgta acgatttaat tccaaaccta atcgaaactg
     7621 ttaaaggcta tgctgcgtac gaagggaaaa cacttgagaa gattgcagaa ctgcgtgctc
     7681 aagttgctaa agctaacaca cctgctgaag caatgacagc aagtaatgag ctaacaagac
     7741 agctttcaag tattttagcg gttgctgaaa attacccaga tttaaaagcc aacaatagct
     7801 ttgttaaact tcaagaagaa ctaactaata cggaaaataa aatttcttat tcacgtcagt
     7861 tatataacac aacaacatct aattataatg taaaattaga aactttccca agtaatattg
     7921 ttggaaaact atttgggttc aaaccaagcc aattcttaga aactcctgaa gaagaaaaag
     7981 aagtgccaaa agtttccttt gacttttaat tgagaaggga gagaggcttt tgctgtatca
     8041 acaaattgca agtaataaga gaaaaacagt tgtcttgtta attgtcttct tctgtctttt
     8101 agcagctatt ggagcagctg taggttattt ggtattaggc agctaccaat ttgggcttgt
     8161 tctagctcta attattgggg ttatctatgc agtaagtatg atttttcaat caactaatgt
     8221 tgttatgtca atgaacaatg cgcgtgaagt tacagaagat gaggcgccaa attattttca
     8281 tattgttgaa gatatggcaa tggtagctca gatacctatg ccacgcgttt tcattgttga
     8341 agatgactcc ctaaatgcct ttgccacagg ctctaagcca gaaaatgctg ctgttgcagc
     8401 aacaacgggc ctattagctg ttatgaaccg cgaagagcta gagggtgtta ttggacatga
     8461 ggttagtcat attcgtaatt atgatatccg tatttcaacc attgcagtag ctttagcgag
     8521 tgctgtaact ctaatatcaa gcattggtag ccgtatgtta ttttacggcg gaggtcgtag
     8581 gcgtgatgat gacagagaag atggaggaaa catcttggtt ttgattttct ctattttatc
     8641 actaatcctt gcaccattag ccgctagttt agttcaatta gctatttcaa gacagcgcga
     8701 atacttagca gacgctagtt ctgttgagtt aacaagaaat ccacaaggga tgatctctgc
     8761 tttagaaaaa cttgatcgtt ctgaacctat ggggcatcca gtagatgatg ctagtgcggc
     8821 tctctatatc aatgacccaa cgaaaaaaga agggttaaag tctttatttt atacgcatcc
     8881 accaattgca gatcgtattg agcgtttgcg tcatatgtag ctgttaacaa aacgtagggt
     8941 agttatgcct tgcgttttgt ttttttaaga caaagccttt aaaacttgat ataatatgac
     9001 atatactata aagggagatg gcacgtgtta cttacagaaa tcaaaaaatc accagagggg
     9061 ctttattttg ataaaaaaat agatatcaaa gagtcgctta tggagcgtca ttcagagata
     9121 atggatattt ctgatattca agtttcaggt catgtggtgt atgaagatgg tctctatcta
     9181 cttgactata atatggctta cgatattaca ttaccgtcta gtcgttctat gaaaccagtt
     9241 gttttatctg aaaagcaaac tattaatgaa gtttttattg aagctgaaaa tgttagcaca
     9301 aaaaaagagc tcgttgatca ggatttggtc ttaatattag aggaagatga tattaattta
     9361 gaagaaagcg ttatcgataa tatcttgtta aatatacctc ttcgtgttct ggcagctgat
     9421 gaggttgggg tagaagcgga tttatcaggt aaaaattggt cgttaatgac tgaaaagcag
     9481 tatgaagaaa aacaagctaa agaaaaagaa aaatctaatc ctttcgcagc cttagaaggt
     9541 atgtttgata gtgatt
//
LOCUS       DBJYSH010000077         9531 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.077, whole genome shotgun sequence.
ACCESSION   DBJYSH010000077 DBJYSH010000000
VERSION     DBJYSH010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 9531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9531
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.077"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            90..728
                     /locus_tag="ACUBV3_001463"
     CDS             90..728
                     /locus_tag="ACUBV3_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HHU3847960.1"
                     /translation="MFLIDFILHIDTHIYAMANTVGNWTYLLLFLVIFVETGAVIFPF
                     LPGDSLLFAAGALAANPKMSFNIVTFLIIFFIAAFIGDSCNFLIGRTFGYRFIKHPFF
                     RRFIKEKNIRDAELYFEKKGTAAIILGRYIPIIRTFVPFVAGISQLPPKVFIKRAFIA
                     ALSWSVIATGSGFLFGNIPFVKQHFSLIILGIVFVTLIPVLISGVKSYRNTK"
     gene            complement(806..1591)
                     /locus_tag="ACUBV3_001464"
     CDS             complement(806..1591)
                     /locus_tag="ACUBV3_001464"
                     /EC_number="3.1.30.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HHU3847961.1"
                     /translation="MKRLHKLFITVIATLGMLGVMTFGLPTQPQNVTPIVHADVNSSV
                     DTSQEFQNNLKNAIGNLPFQYVNGIYELNNNQTNLNADVNVKAYVQNTIDNQQRLSTA
                     NAMLDKTIRQYQNRRDTTLPDANWKPLGWHQVATNDHYGHAVDKGHLIAYALAGNFKG
                     WDASVSNPQNVVTQTAHSNQSNQKINRGQNYYESLVRKAVDQNKRVRYRVTPLYRNDT
                     DLVPFAMHLEAKSQDGTLEFNVAIPNTQASYTMDYATGEITLN"
     gene            1852..3297
                     /locus_tag="ACUBV3_001465"
     CDS             1852..3297
                     /locus_tag="ACUBV3_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HHU3847962.1"
                     /translation="MKKTIGIIGMGVSGLAVLLALSRKKEKELKQLAIFCFDDTVHFG
                     RGIPFQEDVDSALINSPINDISFDYRHMSDFMDWLEENYYDTNLTYVSRTLYGQYLSE
                     RTKLLLDKLPVTVIKTRVDKLNYLSDEQQWQVYIQDKLFPVTFDELHLACGALPVIEP
                     YHLEGKTDYIKDPYPIKYLAQENWKGKTVAIIGTGLAAIDVIKWLLLNTQSSVKAFSR
                     TNYFPSVRIVNDLPLTWHFFTNDNLKAFLSHPSKTFTIADFNDLMSGELEHLGFKNWK
                     NTTETFLSPGIDGLKLAFKYPKQLYLLQQLASRITVWLTDLWPLMSLSDKKEYKALYG
                     KAIINLRNPMPDESAKVILKAAQKGTFSLINNVKTITKNDEGFSIEKEKQNHISVDRV
                     INATGYQLNTKNWTKANPLLKSLINQEFCQIDDQGGITILTENAQVLSPKYGPMPGLY
                     AHGALVNGVVYQNNSTIKIQQMAERAFSDNK"
     gene            complement(3354..4025)
                     /locus_tag="ACUBV3_001466"
     CDS             complement(3354..4025)
                     /locus_tag="ACUBV3_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HHU3847963.1"
                     /translation="MLELKNIAYRYKGNDNKTLENINYSFQSGVFYTILGNSGSGKTT
                     LLSLMAGLDSPTEGQVLFNKKDIKEAGYAQHRKKNIALVFQNYNLLDYLTPLENVQLV
                     KPTADKQLLLDLGLKEDMLTRNILRLSGGQQQRVAIARALVVGTPAILLDEPTGNLDF
                     DIARDITMRLKDFAHKEKRCVIMVTHSREIAHMADTALQLIGDNLKELSKESIAQSIL
                     QQRNL"
     gene            complement(4122..4442)
                     /locus_tag="ACUBV3_001467"
     CDS             complement(4122..4442)
                     /locus_tag="ACUBV3_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HHU3847964.1"
                     /translation="MIAIPAFALSVLISQFIGQKLADSLVYQASRTVRADFMKGFGNM
                     QLGADSDTDMFMQTIKTLNVQVKPEEVWLVILVGLAIVLFAIITTSLIIARYKPRGIL
                     SKLS"
     gene            complement(4451..4579)
                     /locus_tag="ACUBV3_001468"
     CDS             complement(4451..4579)
                     /locus_tag="ACUBV3_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000962057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HHU3847965.1"
                     /translation="MLVVSIILVMMALLFWIGGRTHETGILLAIGKSKAIIIVSTQ"
     gene            complement(4680..4946)
                     /locus_tag="ACUBV3_001469"
     CDS             complement(4680..4946)
                     /locus_tag="ACUBV3_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3847966.1"
                     /translation="MFSKDPVRNSKDKTLVEATIVGIYKGKTVQKSNYPYEMLENTAF
                     SDTQLIKDLYGYSKGKAFYRQTTVYPKKNADIETLKSFIKKQSY"
     gene            complement(5034..5474)
                     /locus_tag="ACUBV3_001470"
                     /pseudo
     CDS             complement(5034..5474)
                     /locus_tag="ACUBV3_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048166.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5484..6713)
                     /locus_tag="ACUBV3_001471"
     CDS             complement(5484..6713)
                     /locus_tag="ACUBV3_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HHU3847967.1"
                     /translation="MNLWTLSLAYLKRQKMKTVTLFLVFLTIGTCLISLMSIQHSLEK
                     NILTKQGKSIYLTSKEKAYWPEQAYEALKKAKMVESVEASLSIDVGSRLKSVSPYNSS
                     KEENQVTLAGYQSTEDLRAFQTKALVLKKGSHLAADNTKQVLVPLKLAQKNHLSVGNK
                     LRLGKENVTIAGIYDANSAKSKNTFNPNIDNTLIAQATLVRKISKQKGYQTVAVRLSD
                     KRLVDTVIQNIKQWPLDFGKLDVQTAKEFYGDSYRNIETLHRLVGRIILIVSLVAMAI
                     LVVMLTFWINNRIKETGILLAIGKTKFEIIGHYLIEVLLVAGAAFTLSIIGGVFLGKT
                     FAAGLLSQVNGGVSSQIVQNSSLIIDRIDNLAVSVGVMDVFRLYAQGALICLFAVVLS
                     SYSILKLQPKQILSRMS"
     gene            complement(6837..7898)
                     /locus_tag="ACUBV3_001472"
     CDS             complement(6837..7898)
                     /locus_tag="ACUBV3_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HHU3847968.1"
                     /translation="MNKITTLSTIALTLMLCVGCSANKDNQKTKTEDPKQENVTANPK
                     EDKATAAKRMKLTKELEKYNKGTESFISNKQYQKYIKTPIKEYGQKVEVKGKKMNVYT
                     VGEGKVPIVFIPGQGTVTAKHQYHNLISNLSKTHKVVVVEPFGSGLSDVIDQPRNLAN
                     ITSDIHEALQKVGITGKYVIASHSIGGVYALKYISTYPKEVLGLIGLDTSTPGMEGGK
                     QVDFAAPVLKELPKIPKVSDDINAQFFAIGHKILNNSNMKEEAKNSSNMINESANYKI
                     PKGIPAMYLLARESDLGLQLRSLDIPIKGSWQEQHKELSEDPKEVSIHTLDGTHQIYD
                     TNLKEVIDYTNQFMKTFEK"
     gene            complement(7933..8952)
                     /locus_tag="ACUBV3_001473"
     CDS             complement(7933..8952)
                     /locus_tag="ACUBV3_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HHU3847969.1"
                     /translation="MNKITKLSTVALALMLCVGCSANKDNQKTKTEQPKQEKSIAKQK
                     QEREAEKAMIAKGAIDLTKYKAKPIEDYGQKIDVGDGKKMNIYTMGQGKIPLVVFPGQ
                     AEISPRYAYKNLLDRLGKKYKIYTVEPLGYGLSDMTDKPRTIENITKEIHTALEKVGV
                     KNFYLMAHSLGGIYGLNYAKTYSNDVKGFIGMDTSTPNMEGDGTTGESSASTLKWAES
                     IPEVDKKTNEQYLSIGAKKSGNKTERDEDRRVSDNLHKMEKVKFPKGMPAKYFLANES
                     AEDIKMRKKIFPKLKSWEQQHIDLSEDPKDVSVETLDASHLIYHTQYKHMAKAIDEWI
                     QSHEK"
     gene            complement(8987..>9531)
                     /locus_tag="ACUBV3_001474"
     CDS             complement(8987..>9531)
                     /locus_tag="ACUBV3_001474"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HHU3847970.1"
                     /translation="GVKNFYLAAHSLGGMYSLNYAKNYPEEVRGFIGMDTSTPWMEGE
                     QKTKYAPESAKWAMKLPDVDDKTNEQYLSIAKKINGNKSQRDEDERLGDNLGKLKNVK
                     FPKGMPTKYFLADQSVEGIKLRQADFKGLKNWDQQHIDLSEDPKDASVEILKGSHNIF
                     QTQYEHMAKAIDGFIQSHQK"
BASE COUNT         2831 a         1792 c         1501 g         3407 t
ORIGIN      
        1 tgataaaata ggctttgtaa gaggccttat aaacaagata gttatcatcc aagtctctgc
       61 actttagttg atcaataaag gagactccta tgtttttaat cgattttatt ttacatattg
      121 atactcatat ctatgctatg gctaacactg ttggtaattg gacctatcta ttgctcttcc
      181 tcgttatttt cgttgaaact ggggctgtca tctttccatt tttaccaggt gatagtttat
      241 tatttgctgc tggagccctt gctgcaaatc caaaaatgtc cttcaatatt gtaacttttc
      301 ttattatttt ctttattgca gcatttattg gtgatagttg caacttcttg attggtcgta
      361 catttggtta ccgttttatc aaacatcctt tctttcgtcg tttcattaaa gaaaaaaata
      421 tccgagatgc tgagctttat tttgagaaaa aaggtactgc tgccatcatt ttagggcgct
      481 atatccctat tatcagaacc tttgttcctt ttgtagcagg tattagtcag ctccctccaa
      541 aagtatttat caaacgcgcc ttcattgcag cattatcttg gtcagttatt gcaacaggtt
      601 ctggttttct ctttggtaat attccttttg tgaagcagca cttctcgctt attatcttgg
      661 gaattgtatt tgttacctta attcctgtcc ttatttctgg tgtcaaatca tatcggaaca
      721 ctaagtaatg tatccaaaaa tttctattta acactattaa tcactaagaa tgacaacaaa
      781 aaaagagcaa ttagttgctc ttttattaat ttagtgttat ttctcctgtt gcataatcca
      841 tagtgtatga tgcttgtgtg tttggaatag caacattaaa ttctaatgtg ccatcttgtg
      901 atttagcttc taggtgcatt gcaaatggaa ctaaatcagt atcattacgg tacaatggag
      961 ttacacggta acgaacacgt ttgttttggt caaccgcctt acgaactaag ctttcataat
     1021 aattttgtcc acgattgatt ttttgatttg attggttgga atgagctgtt tgtgtgacaa
     1081 cattttgagg atttgacacg gaagcatccc aacctttgaa atttccagct aaagcatagg
     1141 caattaaatg ccccttgtcg actgcatgcc cataatggtc attagtagct acttgatgcc
     1201 aacctaatgg tttccaattt gcatcgggaa gagtggtatc tctgcgattt tgatattgac
     1261 gaatggtttt atcaagcatt gcattagcag ttgatagtct ttgttgattg tcaattgtat
     1321 tttgaacata cgctttaaca ttgacatcag catttaaatt tgtctgatta ttatttaatt
     1381 cataaatacc attaacatat tgaaatggta ggttaccaat agcatttttt aaattatttt
     1441 gaaattcctg gctcgtatca acagatgaat tgacatcagc atgtactatc ggcgttacgt
     1501 tttgcggctg cgttggaaga ccaaaggtca ttacccccaa catacctaat gtagcaatta
     1561 cggttataaa cagtttatgt aatcttttca taagctttac ctctttctaa ttacattaaa
     1621 caagcgtcta ccatattgat atccgtttta gctaattgct ctccttttgt cacaaccata
     1681 tcatatgcct attatttaag catgtcaatc atttatttaa tcttatatta atttaatatt
     1741 actttaaatt taatttactc gttaaattag aaaatctgca aataatagct ttgttcatcc
     1801 cctaacttaa atcaaaattt atgctatgat agtggtaagg aggcttgttc tgtgaaaaaa
     1861 acaattggta ttataggtat gggagttagt ggtttagctg ttctactagc actttctcgt
     1921 aaaaaagaga aagagttaaa acagttagct attttttgtt ttgatgatac tgtccacttt
     1981 ggacgaggaa ttccttttca agaagacgtt gactcagctt taatcaattc cccaatcaac
     2041 gatatttcat ttgattatcg ccatatgtct gacttcatgg attggttaga agaaaattac
     2101 tatgacacaa atctaaccta tgtatcaagg acactttacg gacaatacct ttcagaaaga
     2161 actaagttac ttttagataa actccctgtc acggttatta aaacaagagt ggataaacta
     2221 aactatttat cagatgagca acaatggcaa gtctatattc aagataagct ttttcctgtc
     2281 acatttgatg aattacactt agcttgtggt gctctgcctg ttatagaacc ttatcatttg
     2341 gaagggaaga ccgattatat caaagaccct tatcctatca aatacttggc tcaggaaaat
     2401 tggaagggaa aaacagttgc tatcatcggg actggtctag ctgcaattga tgttataaaa
     2461 tggttgctac tcaatacaca atcttctgtt aaagcatttt caagaaccaa ctacttccct
     2521 agcgtccgta ttgttaatga tttaccactt acttggcact tttttaccaa tgataacctt
     2581 aaagcatttc tatcacatcc ttcaaaaacc ttcactattg ccgattttaa tgatttgatg
     2641 tctggcgaat tggagcatct tggctttaaa aactggaaaa atacaacaga aactttttta
     2701 tccccaggaa ttgatggtct caagttagcc tttaaatacc ctaagcaact ctatctatta
     2761 caacaattag cttcacgaat cactgtctgg ttaactgacc tttggccact catgtcatta
     2821 tcagataaga aagagtataa agcactctat ggaaaagcga ttattaactt gcgaaatcct
     2881 atgcctgatg aatctgctaa agtaattttg aaagctgcac aaaaagggac tttttctctg
     2941 atcaataacg tcaagacaat tacaaaaaat gacgaaggat tttcaataga aaaagaaaaa
     3001 caaaatcata tatctgtcga tcgcgtcatt aacgctactg gttaccagtt aaacactaaa
     3061 aactggacaa aagccaatcc acttctaaaa tcgctcatca atcaagaatt ttgtcaaatt
     3121 gacgatcaag gagggattac tattttaact gaaaatgcac aagttttgtc tccaaaatat
     3181 ggtccaatgc caggtttata tgcgcatggt gcactggtta atggtgtcgt ttatcagaat
     3241 aactctacaa ttaaaattca acaaatggca gagcgagcct tttctgataa taaataacca
     3301 tttctaaaaa atacctcctc ttttattatt taagaaaggg aggtattttt ttattataaa
     3361 ttcctttgtt gtaatataga ctgtgctata ctttcttttg ataattcttt taagttatct
     3421 cctatcaatt gtaaagcagt atcagccata tgagcgatct ctctactatg tgtcaccata
     3481 ataacacatc gtttttcctt atgcgcaaaa tctttaagac gcatcgtaat atcacgcgca
     3541 atgtcaaaat ctaaatttcc cgtaggttca tcaagtaaaa tagctggtgt tcctacaacc
     3601 aaagctctcg caatagctac acgttgttgt tgccctcctg ataatctcaa gatatttcgc
     3661 gttaacatat cttcttttaa acctaaatcc aacaataatt gtttatctgc agtaggtttt
     3721 accaactgaa cattttccaa tggggttaga tagtctaaaa ggttataatt ttgaaagacc
     3781 aaagctatat tctttttgcg atgctgagca taacctgctt ctttaatatc ttttttgtta
     3841 aataatactt gtccctcagt aggcgaatca agacctgcca tcaaggatag aagggttgtt
     3901 ttccctgaac cagaattccc taatatagta taaaataccc ctgactgaaa actataattg
     3961 atattttcta atgttttatt gtcgttcccc ttataacgat aagcaatatt ttttaattcg
     4021 agcatagatt tctctttctt gagctatgta ttcaatagca gagcatattc attttaaccg
     4081 ttaatataga tgtcactatt ggtcggtata cttatggcac tttaggacag ttttgataag
     4141 attccccttg gtttgtagcg agcaataatt aaactagtag taataatagc aaataagacg
     4201 attgctaaac caactagaat tactaaccag acttcctcag gcttcacttg tacatttaac
     4261 gtcttaatcg tttgcataaa catatctgta tcactatcag ccccaagctg catattccca
     4321 aaccctttca taaagtctgc tctgacagtt ctagaggcct gatacactaa ggagtctgct
     4381 aatttttgac caataaattg actgataagt actgataaag caaaggctgg aatagcaatc
     4441 attagtactt ctactgcgta ctgacaatga taattgcttt agacttacca atagctaata
     4501 aaatacctgt ctcatgggtt ctaccaccaa tccagaatag cagagccatc atcactagga
     4561 taatagatac tactaacatg ccgccttgta gagaagtaat taattgattc aagacatcta
     4621 tactttttgc ataacttgtt agcaaggttg ctgtaattgt cagttgatat ttattccaat
     4681 caataggatt gttttttgat aaaactttta agagtttcta tatcagcatt ctttttagga
     4741 tatacagttg tttggcgata aaaggcctta cctttagaat agccgtataa atccttaatc
     4801 aattgcgtat ctgaaaaggc tgtattttct agcatctcat aagggtaatt tgatttttgt
     4861 accgttttac ctttatatat gccaacaata gttgcttcaa ctaatgtttt atccttactg
     4921 tttctaacgg ggtcttttga aaacattaac ttatcgccaa ctttaagatg atttgtcttc
     4981 gcaaaagcct gatgaacaat tataactcgc tgatctttcg gcgtgatatg tcgccctccc
     5041 ttaagagaaa tcatccccgc tgtgtattta ggatctgtat cggagtaact attaccatga
     5101 acttgtaaaa cttgttcttt atattgttct ttatcatttg gtcgtgtaac cattttttcc
     5161 gaggttagtc cagcataagc catcaaatta ttattaacgc gcttaacatt tggattactt
     5221 gctatgttaa ctattgcttt ttcagggatt tctccagatc cgtaaccatc accaacaagg
     5281 tcagtactat ccgatgaaat atgcaaaatt gatccagttt accttgaaaa tgtcttctta
     5341 gcagtaagac cagcattttt taccgcaata ccgctaatta atgtagaagc aactagccat
     5401 aaacatacga ataaagtgat actcttacgc tttttacggc taacatataa aaaggaacgt
     5461 ttaaataaat tcatattaac ctcctatgac attcgtgaca atatttgctt tggttgtagt
     5521 ttaagaatag aataactaga taatacaact gcaaataagc aaattaaagc accctgagca
     5581 tagagtctaa agacatccat tactccaaca gaaacagcga ggttatcaat tctatcaata
     5641 attagactac tattttgtac aatttgtgaa ctcacaccac cattaacttg tgataaaagg
     5701 ccagctgcaa atgtttttcc aagaaagaca ccaccaataa tagatagtgt gaaggcagca
     5761 cctgctacta acagtacttc tatcagataa tgacctatga tttcaaactt agtttttccg
     5821 atagcgagta aaattcccgt ctctttaatg cggttattaa tccaaaatgt tagcatgaca
     5881 actaatattg ccatcgccac gagactaaca ataagaatga ttcgtcccac tagacgatgc
     5941 aatgtctcga tattacgata agaatctcca taaaactctt tagctgtttg aacatctaat
     6001 ttcccgaaat caagcggcca ttgtttgatg ttttggataa ctgtatccac taaacgttta
     6061 tcacttaagc gcactgcaac cgtctgataa cctttttgtt tactaatctt cctaaccaat
     6121 gttgcctgag caatcaatgt attatcaata ttgggattaa aagtattttt agacttagca
     6181 ctattcgcat cataaattcc tgcaatagta acattttctt tgcctaatct caatttgtta
     6241 cccacactta aatgattctt ttgtgctaac ttcaatggaa ctagtacttg ttttgtatta
     6301 tcggccgcta aatggcttcc tttctttaag accaatgctt ttgtctgaaa agctcgtaaa
     6361 tcttctgtac tttgataacc tgctaatgta acttgattct cttctttaga agagttatat
     6421 ggtgatacag attttaaacg gctaccaaca tcaatgctta aacttgcctc tactgactca
     6481 accatttttg cttttttcaa agcttcgtat gcttgttcag gccaataagc cttttctttg
     6541 gaggttaaat aaatgctttt cccttgtttt gttaggatat ttttttccaa agaatgctga
     6601 atactcatca gactgattaa acatgtgcct atagttagaa aaactaagaa caaggttact
     6661 gttttcattt tttgacgttt caaataagct aaacttaatg tccataggtt cactattttc
     6721 cctccttttt ctatcttaat aatctctaga ataacgatag aagtcgttag atattctcta
     6781 tcgacttcta cgttatctat caattagtga ttttatagca ctgtttctaa aaacaattat
     6841 ttctcaaatg ttttcataaa ttggttagta taatcaatta cttctttaag gttagtatca
     6901 taaatttggt gagttccgtc tagtgtatga atagagactt cttttggatc ttctgataac
     6961 tccttatgtt gctcttgcca actgccttta attggtatat ctaaactacg taattgaagc
     7021 cctaagtcac tttcacgtgc taataaatac atagcaggaa ttccctttgg aatcttatag
     7081 ttggcacttt cgttaatcat attagaagaa ttcttcgctt cttctttcat attagaatta
     7141 ttaagtatct tatgtccaat agcaaaaaat tgtgcattaa tatcgtcgga tactttagga
     7201 attttaggta attcttttaa cactggagca gcgaaatcaa cttgcttacc accttccata
     7261 cccggcgttg atgtatctaa acctattaat cctagcactt cctttggata agtactgatg
     7321 tatttaaggg cataaacacc accaattgaa tgagaggcaa ttacatattt tccggtaata
     7381 ccaacttttt gtaaagcttc gtggatatct gaggtaatat tagctagatt tcgtggttgg
     7441 tcaatgacat cacttaaacc tgaaccaaat ggctcaacta ccacaacctt atgagtttta
     7501 cttaaattac taatcaaatt atggtactga tgtttggcag tcaccgtccc ttgaccagga
     7561 ataaagacaa ttggtacttt accttctcca acagtataga cattcatctt cttgcctttg
     7621 acttcaacct tttgaccata ttctttgata ggtgttttga tatatttttg atattgtttg
     7681 ttactaataa agctctctgt gcctttatta tatttttcaa gttctttcgt taacttcatt
     7741 cgcttggctg ctgttgcctt gtcttccttc ggatttgcag ttacgttttc ttgcttagga
     7801 tcctcagttt tagttttttg attatcttta ttggcagaac atccaacgca aagcattaaa
     7861 gtcagggcga tggttgataa tgtcgttatt ttattcatct tttctcctta gtaaaataat
     7921 tggggggaat aattattttt catgcgattg aatccactcg tcaatagcct tcgccatatg
     7981 tttatattgc gtatgataaa tcaagtggct agcatctagt gtctcaacag aaacgtcttt
     8041 tggatcttca gataagtcaa tatgttgttg ttcccagctt ttaagcttag ggaatatttt
     8101 tttacgcatt tttatatctt ctgctgactc attagctaag aaatacttag ctggcatacc
     8161 tttagggaac ttaacttttt ccattttatg aagattgtca ctaacacgcc tatcttcatc
     8221 gcgctctgtt ttattacctg attttttagc accgattgat aagtattgtt cattggtttt
     8281 cttatcaact tctggaatgg attctgccca ttttaaggtt gaagctgaag attctccagt
     8341 tgtgccatca ccttccatat ttggtgtgct tgtatccatg ccaatgaaac ctttgacatc
     8401 gttagaatat gtcttagcat aatttagacc gtatatgcca cctaacgagt gagccataag
     8461 atagaaattt ttgacaccga ctttttcaag agctgtatga atctctttgg taatattttc
     8521 aatcgtacgt ggcttatctg tcatatcact aagcccataa ccgagcggtt caaccgtata
     8581 aattttatat tttttcccta atctatcaag caagtttttg taagcataac gtggtgaaat
     8641 ttcagcttga ccagggaaga ctactagcgg aattttccct tgacccattg tatatatgtt
     8701 cattttttta ccgtcaccga catcaatttt ttgtccgtaa tcttcaattg gtttcgcttt
     8761 atatttagtt aggtcaatag cacctttagc aatcatagct ttttctgctt ctctctcttg
     8821 tttttgctta gcgattgact tttcttgctt aggctgctca gttttagttt tttgattatc
     8881 tttattggca gaacatccaa cgcaaagcat caaagcgagg gcaacggttg ataatttcgt
     8941 tattttattc attttttctc cttgacttag gtatttcatt caacgattac ttttgatggg
     9001 attggataaa tccatcaata gctttagcca tatgttcata ttgtgtttgg aaaatattgt
     9061 gactaccttt aagaatttca acggaagcat cttttggatc ttcagataaa tcaatatgct
     9121 gctgatccca atttttcaaa cctttaaaat ctgcttgacg tagcttgatt ccctccacag
     9181 attgatcagc aagaaaatac ttagttggca tccctttagg gaattttaca ttcttcaatt
     9241 taccaagatt atcacctaag cgctcatctt catctcgttg agacttattg ccgttaattt
     9301 ttttcgcaat tgacagatac tgttcgttag tcttatcatc aacatctggt aatttcattg
     9361 cccatttagc actttcaggg gcatactttg ttttttgttc cccttccatc caaggtgtac
     9421 tggtatccat accaataaat cctctgactt cttctggata atttttagca taatttaaag
     9481 aatacatacc gccaagtgag tgagctgcaa ggtagaaatt tttaactcca a
//
LOCUS       DBJYSH010000078         9502 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.078, whole genome shotgun sequence.
ACCESSION   DBJYSH010000078 DBJYSH010000000
VERSION     DBJYSH010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 9502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9502
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.078"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            151..1485
                     /locus_tag="ACUBV3_001475"
     CDS             151..1485
                     /locus_tag="ACUBV3_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HHU3847971.1"
                     /translation="MEDPGSQSLLLQFVILLILTLFNAFFSASEMALVSLNRSKVEQK
                     AEEGDKRYRRLLDVLENPNNFLSTIQVGITFISLLQGASLSASLGHVISGWLGNSATA
                     RTAGSIIALIFLTYVSIVLGELYPKRIAMNLKDRLAIVSAPIIIFLGKIVSPFVWLLS
                     ASTNLLSRITPMTFDDADEKMTRDEIEYMLTNSEETLDAEEIEMLQGIFSLDEMMARE
                     VMVPRTDAFMIDINNDAQSNIEGILSQNFSRVPVYDDDKDRVVGVLHTKRLLEAGFKT
                     GFDTIDLRKILQEPLFVPETIFVDDLLKALRNTQNQMAILLDEYGGVAGLVTLEDLLE
                     EIVGEIDDETDTAEQFVREIDENIYIVLGTMTLNEFNDYFETELESDDVDTIAGYYLT
                     GVGSIPNQEEKVAYEVDSKDKRITLINDKVKDGRITKLKVLLSDIEQNIEKD"
     gene            1553..2341
                     /gene="pflA"
                     /locus_tag="ACUBV3_001476"
     CDS             1553..2341
                     /gene="pflA"
                     /locus_tag="ACUBV3_001476"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HHU3847972.1"
                     /translation="MAEIDYKKVTGMIHSTESFGSVDGPGIRFIIFMQGCKMRCQYCH
                     NPDTWEMETNNSKERTVEDVLKEALRYKHFWGKDGGITVSGGEAMLRIDFITALFIEA
                     KKLGIHTTLDTCGFAYRATPEYHAILEKLLDVTDLVLLDLKEIDPEQHKIVTRQSNKN
                     ILQFARYLSDRGTPVWIRHVLVPGLTDIDDHLKRLGEFVQTLDNVDKFEVLPYHTMGE
                     FKWRELGIPYPLAGVKPPTPERVKNAKDIMKTESYTEYLKRIQN"
     gene            2458..3393
                     /locus_tag="ACUBV3_001477"
     CDS             2458..3393
                     /locus_tag="ACUBV3_001477"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HHU3847973.1"
                     /translation="MSKILVFGHQNPDSDAIGSSVAFAYLAKEAWGLDTEAVALGTPN
                     EETAYVLDYFGVQAPRVVESAKAEGVETVILTDHNEFQQSISDIKDVTVYGVVDHHRV
                     ANFETANPLYMRLEPVGSASSIVYRMFKENGVSVPKELAGLLLSGLISDTLLLKSPTT
                     HASDIPVAKELAELAGVNLEEYGLEMLKAGTNLSSKTAAELIDIDAKTFELNGEAVRV
                     AQVNTVDINDILARQEEIEVAIQEAIVTEGYSDFVLMITDIVNSNSEILALGSNMAKV
                     EAAFEFTLENNHAFLAGAVSRKKQVVPQLTESYNA"
     gene            3453..4106
                     /locus_tag="ACUBV3_001478"
     CDS             3453..4106
                     /locus_tag="ACUBV3_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HHU3847974.1"
                     /translation="MILTFNPGKLERQEFFKELINYLWIHDDVTLRQIKSHFTDYSKI
                     DRLLEEYINHGYILRQNKRYSLNLPFLSSLDGLALDDLVFIDSASQIYQLLQKRKFVT
                     NLDNQTNHLVFVEETDFERNTLTLSNYFYKLTNCYPLSREQKKLYQLLGDVNSEYALK
                     YMSSFILKFLRKDSVKQKRTDIFIQALELLGYISLNQDTTYRLNAKLDVEALKIYLT"
     gene            complement(4136..5137)
                     /locus_tag="ACUBV3_001479"
     CDS             complement(4136..5137)
                     /locus_tag="ACUBV3_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HHU3847975.1"
                     /translation="MIQKNKAPFVLISSVIILLLLILVSISLGYANTSVIDVLKLISG
                     KSDDAFLFIITNIRLPRIIVCIFGGASLGIAGLLLQTLTKNPLADSGILGINAGAGLV
                     IALTIGTFNVSNPTILYFLPLFAMFGGLVTIFLVYLMSYRRNHNISPTRLIITGIGIS
                     TMISGVMISIISQSNNQKMDMIVEWLSGKITISSWTTIITFIPILILLWGLAYSRSRH
                     LNIMNLNEQTALALGLHLKKERIYTLMLASSLAAISVVLIGNITFIGLLAGHLSRRLL
                     GNNHKIILPSCLLIGAIILLVSDTIGRLLLVGTGIPTGLVVSIIGAPYFLWLMTKVD"
     gene            complement(5134..6159)
                     /locus_tag="ACUBV3_001480"
     CDS             complement(5134..6159)
                     /locus_tag="ACUBV3_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000471515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HHU3847976.1"
                     /translation="MFKASKKLVQKNKSNHFWLVFFITLILFLIGCYASLRFGAINFK
                     TSDLITVLKNPLKNSNAQNVIFDIRLPRIIAAILVGAAMSQAGAIMQGVTRNAIADPG
                     LLGINAGAGLALVVAYAFLGSMHYSTILIVCLLGSVISCLLVFTLSYTKQKGYHQLRL
                     ILAGAMISTLFTSVGQVVTLYFKLNRTVIGWQAGGLSQINWKMLIIIAPIIILGLLIS
                     QLLAHQLTILSLNESVAKALGQKTQLMTAFLLLIVLFLSASSVALIGTVSFIGLIIPH
                     FIKLFIPKDYRLLLPLIGFSGATFMIWVDLSSRIINPPSETPISSIISIVGLPCFLWL
                     IRKGKNL"
     gene            complement(6175..7107)
                     /locus_tag="ACUBV3_001481"
     CDS             complement(6175..7107)
                     /locus_tag="ACUBV3_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HHU3847977.1"
                     /translation="MKKIGIIVLTLLTFFLVSCRQQTKEESTKTTISKMPKIEGFTYY
                     GKIPENPKKVINFTYSYTGYLLKLGVNVSSYSLDLEKDSPVFGKQLKEAKKLTADDTE
                     AIAAQKPDLIMVFDQDPNINTLKKIAPTLVIKYGAQNYLDMMPALGKVFGKEKEANQW
                     VSQWKTKTLAAKKDLHHILKPNTTFTIMDFYDKNIYLYGNNFGRGGELIYDSLGYAAP
                     EKVKKDVFKKGWFTVSQEAIGDYVGDYALVNINKTTKKAASSLKESDVWKNLPAVKKG
                     HIIESNYDVFYFSDPLSLEAQLKSFTKAIKENTN"
     gene            complement(7131..7925)
                     /locus_tag="ACUBV3_001482"
     CDS             complement(7131..7925)
                     /locus_tag="ACUBV3_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000018231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HHU3847978.1"
                     /translation="MSHIKAENIIVSYDQKEIINNLSLSILNQKITTIIGANGCGKST
                     LLKALTRIHKIKDGTITIDGHDIAHLPTKEIAKKIALLPQVLEATEGITVYELISYGR
                     FPHQKYLGNLTNDDRSKIHWAMEMTNVAQFANRDVDDLSGGQRQKVWIAMALAQDTDT
                     IFLDEPTTYLDMNHQLEVLELLKKLNNETQKTIIMVLHDLNLSARYSDYLVAMKTGKI
                     IYEGSPSQIMTKDIIKDIFKIDAHIIQDPISKQPVLLSYQLSTDQN"
     gene            complement(8084..>9502)
                     /locus_tag="ACUBV3_001483"
     CDS             complement(8084..>9502)
                     /locus_tag="ACUBV3_001483"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HHU3847979.1"
                     /translation="KNDHNFREILFHEHYYYNWTQNVTFNALSYDSRQISSDTLFFAK
                     GATFKKEYLDSAITAGLSFYVSEIDYGADIPVILVNDIKKAMSLISMSFYNNPQNKLK
                     LLAFTGTKGKTTAAYFAYHMLKVNHRPAMLSTMNTTLDGKSFFKSHLTTPESLDLFRM
                     MATAVENQMTHLVMEVSSQAYLTKRVYGLTFDVGVFLNISPDHIGPIEHPTFEDYFFH
                     KRLLMENSNAVVVNSQMDHFNIVKEQVEYIPHDFYGDYSENVITESQAFSFHVKGKLE
                     NTYNINLIGKFNQENALAAGLACLRLGVSIEDIKNGIAQTTVPGRMEVLTQTNGAKIF
                     VDYAHNGDSLKKLLAVVEEHQKGDIILVLGAPGNKGQSRRKDFGDVINQHPNLQVILT
                     ADDPNFEDPLVISQEIASHINRPVTIIIDREEAIANASTLTNCKLDAIIIAGKGADAY
                     QIIKGNRDNYSGDLEVAKKYLK"
BASE COUNT         3112 a         1516 c         1765 g         3109 t
ORIGIN      
        1 attgctataa aatgccataa acaatattat cgactatgcg ctcagtgcta ttatatgata
       61 taataatagc attatctaat agaagaacag ttggtagagt taatcgctat cagctaaatt
      121 caaataattt ttatttagga gtaattttcc atggaagacc ctggcagtca gagtttgtta
      181 ctacaatttg ttattttatt aatattaacc ctgtttaacg ccttcttttc agcatctgaa
      241 atggctttag tatcgctaaa ccgctctaaa gtagaacaga aagctgaaga aggggacaaa
      301 aggtatcgac gtcttttgga cgttcttgaa aatcccaata attttttatc aacaattcaa
      361 gttggtatta cttttattag ccttttgcaa ggggctagtt tatctgcctc actaggacat
      421 gtgatttcag gatggctagg aaattcagca acagcccgta cagcagggag tattattgcg
      481 ctaatctttt taacatatgt ctctattgtt ctaggagaat tatatcctaa gcgtattgct
      541 atgaatttga aagatcgtct agcgattgtt tcagctccca ttattatctt tctggggaaa
      601 atagtaagcc cctttgtatg gttgctctca gcttcaacta acttattgag tagaatcact
      661 ccgatgactt ttgatgatgc tgacgagaag atgacacgtg atgaaattga atatatgttg
      721 acgaatagtg aagaaacttt ggatgctgaa gaaattgaga tgctgcaagg gattttctcg
      781 ctagatgaaa tgatggcgcg tgaagttatg gttccgcgta ctgatgcttt catgattgac
      841 atcaacaatg atgcacaatc aaatattgaa ggaattttat cacaaaactt ttctcgtgtc
      901 cctgtttatg atgacgataa agatagagtt gttggtgttt tacataccaa gcgcttattg
      961 gaagcgggct ttaaaactgg ttttgatact atcgacttgc gaaaaatact tcaggaacct
     1021 ctttttgttc cagaaacgat ttttgtagat gatcttttaa aagcattacg caacactcaa
     1081 aatcaaatgg ccattttact agatgaatat ggtggcgttg ctggtttagt aactctagag
     1141 gatttattgg aagaaattgt cggcgaaatt gatgatgaaa cagatacagc tgaacaattt
     1201 gtcagggaaa tcgatgagaa tatctatatt gttctaggaa ctatgacgct taatgaattt
     1261 aatgattatt ttgagacaga gttagaaagt gatgatgttg atacaattgc gggttattat
     1321 ttaactggtg tgggtagtat tccaaaccaa gaggaaaaag tagcttacga agtagacagc
     1381 aaagataaac gcatcacttt gattaatgat aaagttaaag atggtcgtat aacaaaattg
     1441 aaagtgctat tgtctgatat agaacagaat attgaaaaag actaagatta cttagtcttt
     1501 ttttcatgaa aatgatataa taaagtcata aaaacggtta caaggtgatg aaatggcaga
     1561 aatagactat aaaaaagtaa caggaatgat ccattctaca gaaagtttcg gctctgtgga
     1621 tgggccaggt attcgtttta tcatttttat gcaaggatgt aaaatgcgtt gtcaatactg
     1681 tcataatcct gatacttggg aaatggaaac taacaattca aaggaacgca ccgttgagga
     1741 tgttctaaaa gaagctcttc gttataaaca tttttgggga aaagatggag gtatcacagt
     1801 ttcaggtggt gaagcaatgc tgcgaattga ctttattaca gctctcttta ttgaggctaa
     1861 gaaactaggt attcatacga cgctagatac ttgtggtttt gcttatcgag caacaccaga
     1921 ataccatgct atccttgaaa aattgttaga tgttactgat ttggtactcc ttgacttaaa
     1981 agaaattgat ccagaacaac ataaaattgt aactcgtcag tctaacaaaa atattttaca
     2041 gtttgctaga tatttatctg atagaggtac tccggtatgg attcgtcatg ttttagttcc
     2101 tggattgact gatattgatg accatttaaa acgtttgggt gaatttgtcc aaacacttga
     2161 taatgtcgat aaatttgaag tattgcctta tcatactatg ggagagttca aatggcgtga
     2221 attaggaatt ccgtatccat tagctggtgt gaaaccacca actccagaac gtgttaaaaa
     2281 cgctaaagat atcatgaaaa cagaatccta tacagaatat ctaaaaagaa ttcaaaatta
     2341 gttttaagtt gaattttatt caactttttt agttgcaaag ggcttcctag taagattttt
     2401 tgctaagtta ttcaattttt gctacaataa ggtgaatata aaagaagagg taaaaacatg
     2461 tctaaaattt tagtctttgg tcaccaaaat ccagattcag atgctattgg atcatcagtt
     2521 gcttttgcat atctagctaa agaagcttgg gggcttgaca cagaggccgt tgctttagga
     2581 actccaaatg aagaaacagc atatgtttta gattattttg gtgttcaagc acctcgagta
     2641 gtagaatctg caaaagcaga gggtgtagag acagtcattt taactgacca taacgaattc
     2701 caacaatcta tttctgatat taaagatgtg acagtttatg gtgttgttga tcatcatcgt
     2761 gttgctaact ttgagactgc caatccgtta tatatgcgtt tggaaccagt tggctcagca
     2821 tcatcgattg tttaccgtat gtttaaggaa aatggagtat cagtacccaa agagttggct
     2881 ggtttattat tatcaggttt gatttcagat actcttcttt tgaaatcacc aacaactcat
     2941 gcttcagata ttccagttgc aaaagaacta gcagagcttg caggtgtaaa cttagaagaa
     3001 tatggtttag aaatgcttaa agcaggtact aacctctcaa gtaagactgc agcggagtta
     3061 attgatattg atgctaaaac atttgaatta aatggagaag ctgttcgtgt tgcacaagtt
     3121 aatactgtgg acattaacga tattttagca cgtcaagaag agattgaagt tgctattcaa
     3181 gaagcaattg tcacagaagg ttattcggat tttgtattga tgattactga cattgttaat
     3241 tcaaattcag agattttagc tctcggttca aatatggcta aagttgaagc agcttttgaa
     3301 tttacacttg aaaataacca cgcttttctt gctggggcag tatcacgtaa aaaacaagtt
     3361 gttcctcaat tgacagaaag ctacaatgct taattaatca tgtaaaacgg gctgcgtccc
     3421 gtttttttag tcatacattt aggagcgttt ttatgatatt aacgtttaat ccaggaaagt
     3481 tagaaagaca agagtttttt aaggagctta ttaactatct ttggatacat gatgatgtaa
     3541 cactaagaca aataaagtcc cactttacag actattctaa aattgaccgt ttgttagaag
     3601 aatatattaa tcatggatat atccttcgcc aaaataaacg ctacagcctt aatcttcctt
     3661 ttctgagttc gctggatgga ttagcattag atgatttggt tttcatagat tctgctagtc
     3721 aaatctatca gttattacag aagcgaaaat ttgtgacaaa tttagataac caaaccaatc
     3781 acttggtatt tgtagaagag acagattttg aaagaaacac tttgactctt tctaattatt
     3841 tctataaact aactaactgt tatccacttt caagagaaca aaaaaagctt tatcaacttt
     3901 taggagatgt taattcggaa tatgctttaa aatatatgtc aagctttata ttgaaatttc
     3961 ttcgcaagga tagtgttaag caaaaacgta cagatatttt tatccaagct ttagaattac
     4021 ttggatatat tagcttaaac caagatacta cctatcgttt aaatgctaaa cttgatgttg
     4081 aagctttaaa gatttactta acataaaaaa agactggaag tttgtccagt cttttttagt
     4141 caacttttgt catcaaccag agaaaatagg gggcaccaat tattgataca accaatccag
     4201 tgggtatacc tgtaccaact aagaggagac gacctatagt atctgatacc aataggataa
     4261 tagcaccaat aagaagacag ctaggtagta taattttatg gttatttcct aatagtcgtc
     4321 tacttaagtg cccagcgagt aagcctataa aagtaatatt accaattaag acgacgctaa
     4381 tagcagcaag gctacttgcc agcatcaaag tatatattct ttcctttttt aaatgaagcc
     4441 ctagagcgag agcagtctgc tcgtttaggt tcataatatt aaggtggcga ctgcgactat
     4501 atgctaatcc ccataacaaa atgagaatcg ggataaaagt gataatggta gtccaggaac
     4561 taattgtaat ttttccacta agccattcaa cgatcatatc cattttttga ttattagact
     4621 gactaataat agaaatcata acgcctgaaa tcattgtaga tattccgata ccagtgatga
     4681 ttaatcgtgt agggctaata ttgtgatttc ttctgtatga cataagatag acaagaaaaa
     4741 tagttaccaa ccctccaaac atagcaaata ggggtaaaaa gtataatata gttggattgc
     4801 taacattaaa tgttcctatg gttagagcga ttactaatcc agctccagca ttgataccaa
     4861 gaataccgga atcagctagt ggatttttgg ttaatgtctg taatagcaac ccagcaattc
     4921 ctaaagaagc tcccccaaag atacagacaa taatacgagg taaacgaatg ttagtaatga
     4981 taaaaaggaa ggcatcatca gattttccac ttattagttt taaaacgtca ataacggaag
     5041 tattagcata gccaagtgaa attgatacta gtattaataa tagtaggata atgacagatg
     5101 atattaaaac aaaaggagct ttattttttt gaatcataga tttttccctt ttctaattaa
     5161 ccataggaaa catggaagcc ctacgatact gattatagaa ctaattggtg tctctgaagg
     5221 gggatttata atacgagatg ataagtctac ccagatcatg aaagtagccc ctgaaaatcc
     5281 tattaaaggt aataacagac gataatcttt gggaataaaa agcttaataa aatgtggaat
     5341 aatcaatcca atgaaagaaa cggttcctat caacgcgact gatgaagccg ataaaaacaa
     5401 aacaataagt aatagaaacg cagtcattaa ttgagttttt tgtcccaatg ctttggccac
     5461 agattcatta aggctcaaga tagttaattg atgtgctagg agttgtgaaa taagtaaacc
     5521 taaaataatg ataggtgcta ttatgattag cattttccag ttaatttggg ataaaccacc
     5581 tgcttgccaa ccgataacag ttcgattgag tttaaagtat aatgtaacca cttgtccaac
     5641 agacgtaaaa agtgtggaga tcatcgctcc agctaaaatg agtcgtagtt ggtggtaacc
     5701 cttttgcttt gtataagaga gtgtgaagac taaaagacaa gaaatgacag accctagtaa
     5761 gcagacaatg agaatcgtac tatagtgcat gctccctaaa aaagcatatg caacaacaag
     5821 ggctaaccca gctccggcgt taatacctag tagtccagga tcagcaatgg cgtttcgagt
     5881 gactccctgc ataatagcac ctgcttgtga catagctgca cctactaaga tagctgcaat
     5941 tatgcgtggt agtctaatgt caaaaataac attctgagca ttagagtttt ttaagggatt
     6001 ttttaaaaca gttattaaat cactagtctt gaaattgata gcaccaaatc gtaagctggc
     6061 gtaacaacca ataagaaata gtataagtgt tatgaaaaaa acaagccaaa aatgatttga
     6121 cttatttttt tgaacgagtt tttttgaagc tttaaacata agaggagctt tcttttaatt
     6181 tgtattttct ttgatagcct ttgtaaatga ttttaattga gcttctaaag atagagggtc
     6241 agagaaataa aacacgtcgt agttactttc tatgatgtgc ccttttttga cagctggtaa
     6301 attcttccag acatcacttt ctttaagtga tgaagctgct tttttagtcg ttttgtttat
     6361 attaacaagg gcataatctc caacgtaatc accgattgct tcttgcgaaa cggtaaacca
     6421 ccctttttta aagacatctt ttttgacttt ttctggggca gcataaccta gtgaatcata
     6481 gattagttct ccaccgcgtc caaaattatt accatataaa tagatatttt tatcataaaa
     6541 atccataata gtaaaagtag tgttaggctt taagatatgg tgtaaatctt ttttggcagc
     6601 gagagtttta gttttccatt ggctaaccca ctgattagct tctttttctt taccgaatac
     6661 tttccccaag gctggcatca tatctaaata attttgtgca ccatatttaa taactaaagt
     6721 tggtgcaatt tttttcagag tattgatgtt tggatcttga tcgaaaacca tgattaaatc
     6781 aggtttttgt gcggcaatag cttctgtatc atcagcagtt aattttttag cttctttcag
     6841 ttgcttacca aaaacggggc tatctttttc taagtctaaa ctgtaacttg aaacattaac
     6901 acctagtttt aataaatacc cagtgtaaga atatgtaaaa ttaattactt ttttcggatt
     6961 ttcaggaatt tttccataat aggtgaagcc ttcaatttta ggcattttag aaatagttgt
     7021 tttagtgctt tcttctttag tttgttgtct gcaagatacc aaaaagaagg tcagtagtgt
     7081 gaggacaata attccaattt ttttcataag ataacatctc ctaaataata ttaattttga
     7141 tcagttgata attgataaga taacaaaaca ggttgtttac taatagggtc ctgaatgata
     7201 tgagcatcaa tcttaaaaat atctttgata atatctttag tcatgatctg actaggacta
     7261 ccttcataga taattttacc ggttttcatt gcaactagat aatcagaata gcgcgcagac
     7321 aggtttaggt cgtgtaggac cataataatt gttttttgcg tttcgttatt aagttttttg
     7381 agcaattcta agacttctaa ttgatgattc atgtcaagat aagttgtagg ttcgtctaaa
     7441 aagatagtat ctgtatcttg tgctaaagcc atagcaatcc atactttttg cctctgacct
     7501 cctgaaaggt catcaacatc tctattagca aattgcgcta cgttagtcat ttccatcgcc
     7561 caatggattt tactacgatc atcatttgtc aaattgccaa ggtatttttg gtgtgggaac
     7621 cgtccataag atattagttc ataaactgtt atcccttcag ttgcttctaa cacctgtggg
     7681 agaagtgcaa tcttcttagc aatttccttt gtgggtaaat gtgcaatatc atgaccatca
     7741 atagtgatag taccatcttt tattttatgg atccgtgtga gagccttcaa aagagtagac
     7801 tttccacatc cattggcacc aataatcgtt gttatttttt ggttaagtat actgagggaa
     7861 agattattga tgatttcttt ctgatcgtaa gatacaataa tgttttctgc ttttatgtga
     7921 gacatagaga acctttctag caaaaaatta agttcattat aacatagaaa aaaggcttac
     7981 aaaagccttt tttagcatta attaagaaaa atgtgaacga aatgataatt atttagaatg
     8041 attcaaaata gtatcaataa tcattattgt ttagcttttt ttatcattta aggtattttt
     8101 tcgcaacttc taaatctcca gagtaattgt cacgatttcc tttgataatt tgataagcat
     8161 ctgccccttt accagcgata ataattgcat ctagtttgca attagtaaga gtactggcat
     8221 tagcaattgc ttcttctcga tcaatgatga tagtaaccgg acgattaata tggcttgcga
     8281 tttcttgtga gataactaag ggatcttcaa aattaggatc atctgcagtg agtatcactt
     8341 gtagattagg atgttgatta atgacatctc cgaagtcttt acgacggctt tgacctttat
     8401 taccaggagc tccgagcact aatataatat cccctttttg gtgttcctca acaacagcta
     8461 ataatttctt caaactgtct ccgttgtggg catagtcaac gaaaatcttt gcgccgttgg
     8521 tttgtgttag gacttccatg cggccaggga cggtagtttg tgcaattccg ttttttatat
     8581 cttcaattga aacaccaagt cggagacaag caagtccagc tgcgagagca ttttcttgat
     8641 tgaattttcc aatcaggttg atattgtagg tgttctccaa ctttcctttg acatggaaag
     8701 aaaaggcctg tgattctgtg ataacatttt cagaataatc tccatagaaa tcatgaggaa
     8761 tatattctac ctgttctttg acaatattaa aatgatccat ctgactattt acaacgacag
     8821 cattgctatt ctccatcaag agccgtttgt ggaaaaaata atcttcgaat gtagggtgct
     8881 cgataggacc aatatgatcg ggactaatat ttaagaaaac gccaacgtca aaggttaaac
     8941 cataaacacg tttggttagg tatgcttggc ttgatacttc catgactaag tgtgtcattt
     9001 gattctcaac ggctgtagcc atcatccgaa ataaatcaag actctcgggg gttgtgagat
     9061 gtgatttaaa gaaagattta ccatctaatg ttgtgttcat agttgagagc atagcaggac
     9121 gatggtttac ttttagcata tgataagcaa agtaagccgc agttgttttt ccttttgttc
     9181 cagtaaaagc tagaagttta agtttgtttt gtgggttatt atagaaagac atagatatca
     9241 aactcatggc ttttttgata tcattgacta atatcactgg aatatcggcg ccgtaatcta
     9301 tctcagaaac gtaaaaagaa aggcctgctg tgattgcaga atctagatat tcttttttaa
     9361 aagtggcacc cttagcaaaa aataaagtat cagatgaaat ttgacgacta tcatatgata
     9421 aggcattaaa agttacattt tgtgtccagt tataataata atgctcatga aacaaaatct
     9481 ctctgaaatt atggtcattt tt
//
LOCUS       DBJYSH010000079         9402 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.079, whole genome shotgun sequence.
ACCESSION   DBJYSH010000079 DBJYSH010000000
VERSION     DBJYSH010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 9402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9402
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.079"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            51..1166
                     /locus_tag="ACUBV3_001484"
     CDS             51..1166
                     /locus_tag="ACUBV3_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000703866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK signal domain/LPXTG anchor domain surface
                     protein"
                     /protein_id="HHU3847980.1"
                     /translation="MKISQYNKWSIRRLKVGAASVMIASGSIVALGQSHIVSADEMSQ
                     PKTTITAPTANTSTNVESSTDKALSKVTTMETSSEMPKMQNMAKVEKTSDKPMMVATS
                     VRKMMATPTPVAMTKTTSVDEVKKSTDTAFKQTVDVPAHYVNAAKGNGPFLAGVNQTI
                     PYEAFGGDGMLTRLILKASEGAKWSDNGVDKNSPLLPLKDLTKGKYFYQVSLNGNTAG
                     KKGQALLDQIKANGSHTYQATITIYGTKDGKVDMNTILGQKTVMIHINVAKKDMNSTS
                     MMMKKDKMTMPMKKEMTSSKINTGMMMSNNKMSANMQMSSQAKSNDKAGKKMSMMSKN
                     LPNTGETKQQNVGVLGMLSLAFATGLTALGLKKSKQR"
     gene            1279..1965
                     /locus_tag="ACUBV3_001485"
     CDS             1279..1965
                     /locus_tag="ACUBV3_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HHU3847981.1"
                     /translation="MTQKLLLVDDEFEIIDINRRYLEQAGYEVSVAADGIEALKEVDE
                     NRFDLIISDIMMPKMDGYDFISEVLVREPNQPFLFITAKVSEPDKIYSLSMGADDFIS
                     KPFSPRELVLRVKNILRRIYGNHQQSEVLTIGDLVIDQKQRLVMVDCNTISLTNKSFD
                     LLWILANHLNRVFSKTELYERVWGEEFLDDTNTLNVHIHALRNDLAKFSTDNTPTIKT
                     VWGLGYKLEE"
     gene            1967..3004
                     /locus_tag="ACUBV3_001486"
     CDS             1967..3004
                     /locus_tag="ACUBV3_001486"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HHU3847982.1"
                     /translation="MKLKYYIVIGYLISMLITVAGVFFGLNHMLIETRGVYYILSVTI
                     IACIVGGIVNLFLLSSVFTSLKKLKQKMKDISQRRFDTKAQICSPQEFKDLETAFNQM
                     SSELESTFKSLNESEREKTMMIAQLSHDIKTPITSIQSTVEGILDGIISEEEVNYYLN
                     TISRQTNRLNHLVEELSFITLETMSDTAEPHKEETIYLDKLLIDILSEFQLVFEKENR
                     QVMIDVAPDVSKLSSQYDKLSRILLNLISNAVKYSDPGSPLTIKAYSNRQDIVIDIID
                     QGYGIKDEDLASIFNRFYRVESSRNMKTGGHGLGLYIARQLAHQLNGDILVESQYQKG
                     SKFSLVLKLQK"
     gene            complement(3018..3758)
                     /locus_tag="ACUBV3_001487"
     CDS             complement(3018..3758)
                     /locus_tag="ACUBV3_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HHU3847983.1"
                     /translation="MTNSEIKNEAKTILSNLQGKNQLFLLPILLSIITLYISFYYQYN
                     NMTLLDFFVPLPVYFFYTLFIISVSFVMLDVVKNQKLNVRFSDNTYVFSSHIFWKLLS
                     VLVLKGLILSFFYLLSTFGLLIIISSFRLLLSGNLILAPVLIVVSSLITTKAVIKLVQ
                     QYYSYSISTLVFYTQLESGNYEGPSKVLVASRELMNGNKLRLFLLDLSFIGWHFLTIF
                     SFGLVYIYLLPYQTTARLIFYRNITKNS"
     gene            3945..5087
                     /gene="tgt"
                     /locus_tag="ACUBV3_001488"
     CDS             3945..5087
                     /gene="tgt"
                     /locus_tag="ACUBV3_001488"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HHU3847984.1"
                     /translation="MTDHPIKYRLIKQEKHTGARLGEIITPHGTFPTPMFMPVGTQAT
                     VKTQSPEELKEMGSGIILSNTYHLWLRPGDELIAKAGGLHKFMNWDQAILTDSGGFQV
                     YSLADSRNITEEGVTFKNHLNGAKMFLSPEKAISIQNNLGSDIMMSFDECPQFYQPYD
                     YVKKSIERTSRWAERGLNAHRRPHDQGLFGIVQGAGFEDLRRQSARDLVSMDFPGYSI
                     GGLAVGETHDEMNAVLDFTVPMLPNDKPRYLMGVGAPDSLIDAVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKFAEDFTPLDPNCDCYTCKNYTRAYIRHLLKADETFGIR
                     LTSYHNLYFLVNLMKDVRQAIMDDNLLEFRQDFMERYGYGTNNRNF"
     gene            5193..5504
                     /locus_tag="ACUBV3_001489"
     CDS             5193..5504
                     /locus_tag="ACUBV3_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000983195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHY zinc finger protein"
                     /protein_id="HHU3847985.1"
                     /translation="MMQEYFGIGLDSSSRCYHYHTKLDIVALKCAVCQKYYACYKCHD
                     ALEEHCFAATKSDEMFPVLCGSCRQMLTLKEYKTGFCPYCRMLFNPNCQRHDSIYFSK
                     E"
     gene            5511..6050
                     /locus_tag="ACUBV3_001490"
     CDS             5511..6050
                     /locus_tag="ACUBV3_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HHU3847986.1"
                     /translation="MFHTKSLIQMAMMATLLVVLGFIPALPLGFIPVPIVLQNLGVMM
                     AGVILGGRKGSLSILLFFLVGLVIPVFSGLRSTIPVLTGPTAGYVIAWLFVPLLISYG
                     VKLVNKKNFIAIFLIVWLAGVLFVDVAGAVWLASYTNIPLDKSLLQNLVFLPGDTIKA
                     IIAAVVAVKYKDSFFLTEK"
     gene            6189..6965
                     /locus_tag="ACUBV3_001491"
     CDS             6189..6965
                     /locus_tag="ACUBV3_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001932415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HHU3847987.1"
                     /translation="MKLTTLGCWGGYPYKDGGTTSFLLTGENNFHLLIDAGSRSINEL
                     EKEISPLDLDAVIISHYHPDHVADLGVLYHYRLLYPKDKWEPKVLPVYGHNEDDYEFS
                     KLTLPNVAQGIAYHTNGVEQVGPFNITFIKTVHPVVCYAMRITERETGQVLVFTGDSG
                     YFADLVPFTKGADLFLADVYLYEGNENHIAHLTSKEAGEIAYNAKVKKLVLTHVPQFP
                     GEGINPENHLEELRLQSEKYAGGISVDLAQPHKSWDLGKL"
     gene            6965..7471
                     /gene="tadA"
                     /locus_tag="ACUBV3_001492"
     CDS             6965..7471
                     /gene="tadA"
                     /locus_tag="ACUBV3_001492"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001169006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HHU3847988.1"
                     /translation="MQKFTQEEKEWFMTEALKEAKSSLEKEEIPIGCVIVKDGHIIGR
                     GHNAREEFNKAILHAEIMAINNANEKVGNWRLLDTTLFVTVEPCVMCSGAIGLARIPH
                     VIYGAKNTKFGAAGSLYDILSDSRLNHRVEVETGILEDQCAKIMQDFFRKGRQRKKEA
                     KLSEKTKI"
     gene            7506..7591
                     /gene="ffs"
                     /locus_tag="ACUBV3_001493"
     ncRNA           7506..7591
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACUBV3_001493"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            7743..9092
                     /locus_tag="ACUBV3_001494"
     CDS             7743..9092
                     /locus_tag="ACUBV3_001494"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HHU3847989.1"
                     /translation="MTHITFDYSKVLGQFVGEHELDYLQPQVSAADAFLRQGTGPGSD
                     FLGWMDLPENYDKEEFSRIQKAAEKIKSDSEVLVVIGIGGSYLGAKAAIDFLNNHFAN
                     LQTAEERKAPQILYAGNSISSTYLADLVEYVQDKEFSVNVISKSGTTTEPAIAFRVFK
                     ELLVKKYGQEEANKRIYATTDKVKGAVKVEADANNWETFVVPDNVGGRFSVLTAVGLL
                     PIAASGADITALMEGANAARKDLSSDKISENIAYQYAAVRNVLYRKGYITEILANYEP
                     SLQYFGEWWKQLAGESEGKDQKGIYPTSANFSTDLHSLGQFIQEGYRNLFETVVRVEK
                     PRKNVTIPELTEDLDGLGYLQGKDVDFVNKKATDGVLLAHTDGGVPNMFVTLPTQDAY
                     TLGYTIYFFELAIGLSGYLNSVNPFDQPGVEAYKRNMFALLGKPGFEELSAELNARL"
BASE COUNT         3186 a         1456 c         1807 g         2953 t
ORIGIN      
        1 ttattttttg ttggtatact ctcattaaca aaaagaaaga ggaatccctt atgaaaatat
       61 cacaatacaa taaatggtca attcgccgat tgaaagttgg agctgcatct gtaatgattg
      121 ctagtggaag cattgttgct ttagggcaat ctcatattgt ttcagcagat gagatgtccc
      181 aacctaaaac aaccattaca gccccaacag ctaacacttc tacaaatgta gaaagtagta
      241 ctgataaggc attatcaaaa gttactacga tggagacaag ttcagaaatg cctaaaatgc
      301 aaaatatggc aaaagttgaa aaaacgagtg acaaacctat gatggtggct acctctgtta
      361 gaaaaatgat ggcaacgccg acacctgtag cgatgaccaa aacgactagt gtagatgagg
      421 taaagaaatc aactgatact gcttttaaac agacagtaga cgttccggct cattatgtta
      481 atgcagcaaa agggaatggc ccatttctag caggtgtaaa tcaaacgatt ccatatgaag
      541 cgtttggtgg agacggcatg ttaacgcgtt taatccttaa agcttcagag ggagcaaaat
      601 ggtcagacaa tggtgttgat aaaaatagtc cattattacc gttaaaagat ttaactaagg
      661 gtaaatactt ctaccaagtc tctcttaatg gcaatacagc cggtaaaaaa ggccaagctc
      721 tcttagatca aattaaggca aatggaagcc atacttatca agcaactatc actatttatg
      781 gtacgaaaga tggcaaagtt gatatgaata ctattttagg acaaaagaca gtgatgattc
      841 atattaatgt cgctaaaaaa gatatgaata gcacatcaat gatgatgaaa aaagataaga
      901 tgacaatgcc tatgaagaaa gaaatgactt catctaaaat aaataccggt atgatgatgt
      961 ctaacaacaa gatgtcagct aatatgcaaa tgtcatcaca agccaaatca aatgataaag
     1021 ctggtaaaaa aatgtctatg atgtctaaaa atttacctaa tacaggtgaa acaaagcaac
     1081 aaaatgtagg tgtgctaggt atgcttagtc tagcctttgc gacaggtcta actgcgctag
     1141 gccttaaaaa atctaaacaa agatagggat agtatttaat tcagaacacc aaatttgttt
     1201 aaaattattg agggttatcg tggtaaaata gtgttatact agaggtatag cactattttg
     1261 actcattagg agagtagaat gacacaaaaa ttattattag ttgacgatga atttgaaatt
     1321 attgatatta atagacgtta tctagagcaa gcaggatatg aagtgtcagt agctgcggat
     1381 ggcatagaag cactcaaaga ggtagatgag aatcgttttg acctaattat tagtgatatt
     1441 atgatgccta aaatggatgg ctatgatttt attagtgaag ttctagttcg agaacccaac
     1501 caaccttttc tctttataac cgctaaagta tctgaaccag ataaaattta ctctttgagt
     1561 atgggtgcag atgactttat ttccaagcct ttcagtccta gggaattagt tttacgtgtt
     1621 aaaaatattc taagacgtat ctatgggaat catcaacaat ctgaagtatt gacgattggt
     1681 gatttggtta tcgaccaaaa acaacgtttg gtaatggtag actgcaatac aatttcctta
     1741 accaataaat catttgactt gctttggata ttagcaaatc atttgaatcg tgttttttca
     1801 aaaacagagc tttatgaacg tgtctggggc gaagaatttt tagatgacac taatactttg
     1861 aatgttcata tccatgcctt acgcaatgac cttgctaaat ttagtacaga caatacccca
     1921 actatcaaaa cagtttgggg attagggtat aaattagagg aataaaatga aattaaaata
     1981 ttatattgtt attggttacc ttatttctat gttaataact gtagcaggtg ttttctttgg
     2041 gcttaatcat atgctgattg agactcgagg tgtttactac atcctttcag ttacaattat
     2101 tgcctgtatc gtaggtggta tagttaattt gttcttatta tcaagtgtat ttacatcttt
     2161 gaaaaagtta aaacaaaaaa tgaaggatat ttctcaaaga cgttttgata caaaagcaca
     2221 aatttgttca ccacaagaat tcaaggattt agagacagcc tttaaccaaa tgtcttcaga
     2281 gttagagtca acgtttaaat ctctaaacga gagtgaacgc gaaaaaacaa tgatgattgc
     2341 acaactttca catgatatca agactccaat tacctctata caatcaacag tagaaggtat
     2401 tttagatggt attatatctg aagaagaagt taactattat ctcaatacca tttctcgtca
     2461 aacaaaccgt cttaaccatc tagttgaaga attaagtttt attacattag agactatgtc
     2521 agacacggct gaacctcata aagaagaaac aatttatctg gataaactac tgattgatat
     2581 tttatctgaa tttcaattgg tgtttgaaaa agaaaatcgg caagtaatga ttgatgttgc
     2641 gccagatgtt tcgaaattat ctagtcaata tgataaatta tcacgaattt tattgaattt
     2701 gattagcaat gcagtcaaat attctgatcc tggaagtccc ttgactatca aggcctattc
     2761 aaatcgtcaa gatatcgtta tcgatattat tgaccaaggc tatgggataa aagatgaaga
     2821 tcttgcttct atttttaatc gtttttatcg tgtagaatct tcacgaaata tgaagacagg
     2881 aggacatggc ctaggtcttt atattgcgcg acaacttgca catcaactga atggtgatat
     2941 tttggtcgaa agtcagtatc aaaaaggcag taagttctct ttagtactta aattacaaaa
     3001 atagctagga attattccta gctatttttt gtaatgtttc tataaaagat taagcgcgca
     3061 gtagtttggt aaggaaggag atagatatag actaatccga aactgaagat tgtaaggaaa
     3121 tgccaaccaa taaaacttaa gtctaaaagg aagagtctaa gtttattacc attcattagt
     3181 tctcgactag caactagtac tttagatggt ccctcatagt ttccactttc taattgagtg
     3241 taaaatacta aagttgagat actataggag taatattgtt gaacgagttt tataactgcc
     3301 ttagtagtaa ttaggctact aacaactatt aaaacagggg caaggataag gttaccactc
     3361 aaaagtagcc taaaacttga aatgatgatt agtagaccaa aagtacttag tagataaaag
     3421 aaagataaaa ttagtccttt tagaacaagt actgagagta atttccagaa aatatggctt
     3481 gaaaatacat aagtattgtc cgagaatcta acgtttagtt tctgattttt gacgacatct
     3541 aacattacaa aactgactga aataataaaa agtgtgtaaa agaaataaac tggaagaggt
     3601 acaaaaaaat caagtagtgt catattatta tattgataat aaaatgatat atataacgta
     3661 ataatactaa gaagaatcgg gagtaaaaaa agttgattct taccttgaag attgcttaat
     3721 atagtttttg cctcattttt aatttctgaa ttcgtcatat ctcctccata cataaaagta
     3781 caattcattt cattataaca cattccttaa gatgagcatg acaagagtac atttttttga
     3841 taaaatctta atggctaaaa aagaaattaa tacaaagtat tacatttaga tattgattag
     3901 cactaaagcc catgactgtt tttcatggaa gcaaggagaa aacgatgact gatcatccaa
     3961 taaaatatcg attaattaaa caggaaaaac acacaggagc cagacttggt gaaataatca
     4021 caccacatgg tacatttccg acaccaatgt ttatgcctgt tgggacacaa gcaactgtta
     4081 agacacaatc tcctgaagaa ctaaaagaaa tgggttcagg aattattcta tcaaatacct
     4141 accatttatg gttacgtcca ggtgatgaat tgattgctaa agctggaggt ttacataaat
     4201 tcatgaactg ggatcaggct attctaacag acagtggagg ctttcaggtt tattctttgg
     4261 ctgatagtcg aaacatcact gaagaaggag taacctttaa aaaccattta aatggtgcga
     4321 aaatgttttt atcaccagaa aaagctattt caattcaaaa taaccttggt tctgatatta
     4381 tgatgagttt tgatgagtgt ccacaatttt accagcctta tgattatgta aaaaaatcaa
     4441 ttgagcgaac aagccgttgg gcagaacgtg gattaaatgc ccaccgccgt cctcatgatc
     4501 aaggactatt tggtattgtt caaggggcag ggtttgagga tttacgtcgt caatcagcac
     4561 gagatttagt atcgatggat tttccaggtt attcaattgg agggcttgcg gtcggagaaa
     4621 cacatgatga gatgaatgca gttcttgatt ttactgttcc aatgcttccg aatgataagc
     4681 ctcgttattt gatgggagtt ggtgcaccag atagtttaat agacgccgtt atccgaggag
     4741 tagatatgtt tgactgcgtc ttacctactc gtattgctcg aaatgggaca tgtatgacaa
     4801 gtcaaggtcg tttagttgtt aagaatgcta aatttgcaga agattttacg cctcttgatc
     4861 caaattgtga ttgctatact tgtaaaaatt atacacgcgc ttatatccgt cacttattga
     4921 aagcagatga aacttttggt attcgtttga ctagttacca taatctttat ttccttgtca
     4981 atcttatgaa agatgtcaga caagctatta tggatgataa tcttctagag ttccgtcaag
     5041 attttatgga gcgctatggt tatggcacga ataaccgtaa tttttaaaaa agttcctggt
     5101 aaaacagggc tttttttgat ctgtttgtta acctcgtttt aaaaaaagtt gacaatggaa
     5161 aaacttttgt ataatatgtt ataggaggaa tcatgatgca agagtacttt ggtattggtc
     5221 ttgatagtag cagccgctgt taccactatc atactaaatt ggatattgtc gctttaaaat
     5281 gtgctgtttg tcaaaaatat tatgcttgtt ataaatgtca tgatgcactc gaggagcatt
     5341 gttttgcagc aacaaaatct gatgaaatgt ttcctgtctt atgcggttct tgtcgtcaga
     5401 tgttgacatt aaaagagtat aaaactggtt tttgtcctta ttgtcgtatg ctttttaatc
     5461 caaattgtca acgccatgat tctatttatt tttcgaagga gtaattttta atgtttcaca
     5521 caaaaagcct aatccagatg gctatgatgg ctactttatt ggtggtactt ggtttcattc
     5581 ctgctctacc acttggtttc attccggtcc ctatcgtctt acaaaaccta ggggttatga
     5641 tggctggtgt tattttgggc ggaagaaaag ggagtttatc tattttgctc ttctttcttg
     5701 tcggtttagt tattcctgta ttctcaggtt tacgttcgac tatacctgta ttgacaggac
     5761 caacagctgg ttatgttatt gcttggttgt ttgttccttt gcttatctca tatggtgtaa
     5821 aattagtaaa taaaaagaat tttatagcta ttttccttat tgtatggcta gcaggggtcc
     5881 tttttgtaga tgtagcagga gcagtttggc tggcatctta tacgaacatc ccattagata
     5941 aatcacttct tcaaaactta gtatttctcc caggagatac aataaaagcc attatagctg
     6001 ctgttgttgc tgttaaatat aaagatagtt ttttcttgac tgaaaaataa tcaagattgc
     6061 tatattttag atggactagt agatctaaaa atttagattt actagtcttt tttgtgcata
     6121 aaatctaaaa cagttattct taacaaaatt tggtataata gcattaaatc agtaaggagg
     6181 ggattatcat gaaattaaca acgttaggtt gttggggtgg ttatccttat aaagatggag
     6241 gaaccacaag ttttctatta actggagaaa ataattttca cttattaata gatgcgggaa
     6301 gtcgttcaat caatgagtta gaaaaagaaa taagcccgtt ggatttggat gcagttatca
     6361 tcagtcatta tcatccagat catgtagcag atttgggtgt gttgtatcat tatcgtttgc
     6421 tataccctaa agataaatgg gaacccaaag tattgccagt ttatggacat aatgaggatg
     6481 attatgagtt ttctaaattg acattaccta acgttgctca aggaattgct tatcatacca
     6541 atggagttga gcaagttggt cccttcaata ttacgtttat caaaacagtc cacccagtcg
     6601 tctgctatgc catgaggatt acagaaagag aaactggtca agtattagtc tttacaggag
     6661 attctgggta ttttgctgac cttgtacctt ttacaaaagg cgcagattta tttttagcag
     6721 acgtttatct ttacgaggga aatgaaaatc atattgcaca tttgactagt aaagaagcag
     6781 gtgagattgc ttataacgct aaagttaaga aattagtttt aacacatgtt ccgcagtttc
     6841 cgggagaagg aataaacccc gaaaatcatt tggaagaact tcgactacag tctgaaaagt
     6901 atgcaggtgg catctctgtt gatttagctc agccacataa atcttgggac ttaggaaaac
     6961 tataatgcag aaatttaccc aagaagaaaa agaatggttt atgacagaag ctctaaagga
     7021 ggcaaaatcc tccttagaga aagaagaaat tcctataggt tgtgtcattg taaaagatgg
     7081 tcatattatt ggaagaggac ataatgctcg tgaagagttt aataaagcta ttttacatgc
     7141 tgaaatcatg gctattaaca atgcaaatga gaaggtaggg aattggcgct tattagatac
     7201 tactttattt gttactgtag aaccttgtgt aatgtgtagt ggagctattg gcttagctag
     7261 aattccccac gttatatatg gcgctaagaa tacgaaattt ggagcagcgg gaagtctcta
     7321 tgatatttta agtgatagcc gacttaatca tagagttgaa gttgaaaccg gcattttaga
     7381 agatcaatgc gctaaaataa tgcaggattt ctttcgaaaa ggacgacaac gcaagaaaga
     7441 agctaaacta agtgaaaaaa ctaagatttg atagcttgca ataaaatatt agttttgtta
     7501 taatatcttt ggagcaacat ttactcgtga agtgggtcag gggaggaatc cagcagccct
     7561 aagcgatgct aaatgtgtgc tctttttatt ttcaaaagtc aaagtgtttt tggaaataat
     7621 tatgaaagtt tcataaaaat tggtagcgtt tcaaaaaata tgaaaataca gttaaaaaac
     7681 cttgaaaaaa aactcacaaa agagtaagat gatagtgaaa cataattgtt aggagaaata
     7741 aaatgacaca tattacattt gactattcaa aagtcttggg tcaatttgta ggcgaacacg
     7801 agttagacta cctacaacca caagtaagtg cagcagatgc tttccttcgt caagggactg
     7861 gtcctggatc agattttctc ggatggatgg acctcccaga aaactatgac aaagaagaat
     7921 tttctcgcat tcaaaaagcc gctgaaaaga ttaaatcaga tagcgaagta ctcgtggtta
     7981 ttggtattgg tggttcgtac cttggtgcaa aagcagcaat tgactttttg aataatcatt
     8041 ttgctaattt gcaaaccgca gaagaacgta aagcgcctca gattctttat gctggaaatt
     8101 ctatttcatc tacttacctt gccgatttag ttgaatacgt ccaagataaa gaattctcag
     8161 taaatgtcat ttcaaaatca ggtacaacaa ctgaaccagc gattgctttc cgtgtattta
     8221 aagaacttct agttaaaaag tacggtcaag aagaagctaa taaacgtatc tacgctacaa
     8281 ctgataaagt taaaggtgcc gtaaaagttg aagcagatgc taacaattgg gaaacatttg
     8341 tagttcctga taatgttggt ggtcgtttct cagtactgac agctgtaggt cttcttccaa
     8401 ttgctgcttc aggagcagat attactgctc ttatggaagg cgcaaatgct gctcgtaaag
     8461 atttatcttc agataaaatc tctgaaaaca ttgcttatca gtacgctgca gttcgtaatg
     8521 ttctttatcg caaaggttat atcacagaga ttttagctaa ctacgaacca tcattacaat
     8581 actttggtga atggtggaaa caattagcag gtgaatcaga aggtaaagat caaaaaggta
     8641 tctatcctac atcagcaaac ttctcaactg atcttcactc acttggtcaa tttattcaag
     8701 aaggttaccg taacctcttt gagacagttg ttcgcgttga gaaacctcgt aaaaatgtga
     8761 ctattcctga attaaccgaa gatttagatg gtcttggtta tcttcaagga aaagatgtag
     8821 attttgttaa taaaaaagca acagatggtg tgcttcttgc tcatacagat ggtggggttc
     8881 caaatatgtt tgtaacgctt cctacacaag acgcttacac tcttggttac actatttact
     8941 tctttgagtt agcaattggc ctttcaggtt atcttaactc agtaaatcca tttgatcaac
     9001 cgggggtaga agcatataaa cgtaatatgt ttgctcttct aggtaaacct ggattcgaag
     9061 agcttagcgc tgaattgaat gcacgtcttt aaaaatatct aactaaaaag ttcctcaaag
     9121 agaggaactt tttagttttg ttgtaggata actaaaaacc cactagcact ggtagcgata
     9181 gtgggttgga aaaagggttg agaaaagtta ttaacttttc tcaggagatt tatgaaaaag
     9241 gaaaataatt taggataata ttttcctaac ggctaggata aaccgcttat tttaggatga
     9301 tgctagtata cagtagaaac cttaaataaa ccttaaaaat tcatacttta tcattttatt
     9361 ttcccctata ctatttttgt ataaatggtc aatattaatg gt
//
LOCUS       DBJYSH010000080         9340 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.080, whole genome shotgun sequence.
ACCESSION   DBJYSH010000080 DBJYSH010000000
VERSION     DBJYSH010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 9340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9340
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.080"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            <1..375
                     /locus_tag="ACUBV3_001495"
     CDS             <1..375
                     /locus_tag="ACUBV3_001495"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019318858.1"
                     /note="catalyzes the transfer of phosphate between the C1
                     and C5 carbons of pentose; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HHU3847990.1"
                     /translation="MGLSEFEKGFSFTNLVDFDALYGHRRDPHGYRDCLHEFDERLPE
                     IVSAMRDKDLLLITADHGNDPTYAGTDHTREYIPLLAYSPSFTGNGLIPVGHFADISA
                     TVADNFGVDTAMIGESFLQDLV"
     gene            426..833
                     /gene="arsC"
                     /locus_tag="ACUBV3_001496"
     CDS             426..833
                     /gene="arsC"
                     /locus_tag="ACUBV3_001496"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416958.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HHU3847991.1"
                     /translation="MEKVRIYHNPNCGTSRNVLAIIRHCGIEPEIIYYLKTPPSKMAL
                     VELLLEMKLSVRELLRTDVPAYEKFNLESSSVTDEEMIDAMIQDPVLINRPIVVTSKG
                     AKLCRPCEEILTILPVKMEKDFVKEDGQIIQSL"
     gene            872..1681
                     /locus_tag="ACUBV3_001497"
     CDS             872..1681
                     /locus_tag="ACUBV3_001497"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HHU3847992.1"
                     /translation="MTLLEKINETRDFLQAKGVTAPEFGLILGSGLGELAEEIENPIV
                     VDYADIPNWGQSTVVGHAGKLVYGDLSGRKVLALQGRFHFYEGNTMEVVTFPVRIMRA
                     LACHSVLVTNAAGGIGYGPGTLMLIKDHINMIGTNPLIGENLEEFGPRFPDMSDAYTA
                     TYRQKAHQIAEKQNIKLEEGVYLGVSGPTYETPAEIRAFQTMGAQAVGMSTVPEVIVA
                     AHSGLKVLGISAITNFAAGFQSELNHEEVVEVTQRIKEDFKGLVKSLVAEL"
     gene            1683..2939
                     /locus_tag="ACUBV3_001498"
     CDS             1683..2939
                     /locus_tag="ACUBV3_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HHU3847993.1"
                     /translation="MVSRLYYAVKFMIAVLFMTVMAGVGAILMHYVLMFTEWLAFGDS
                     RENTLSLLNSVTPIKRVLSLTLVSFLASLSWYYLQIKPKQITSIKQQVVFKDFSVKKS
                     PYWLHIGHAFLQLIYVGAGGPIGKEGAPREFGAINAGKISDLLALKVLDKRLLIVSGA
                     AAGLSAVYQVPLASVFFAFETLALGISLKNIVTLLVSTFGAASIAQLVISTAPLYHIS
                     KMSLNSQSLAFMFLIVLCVTPIAISFRYLNQKVTERRIKNIKILLSLPVVSLIVSVLS
                     IIYPQILGNGNALVQEVFKGTTVSLIAILVVLKMIATLSTLYAGAYGGILTPSFSIGA
                     CLGFLLASISIPLLPHLSIVTSMLVGAAIFLAITMRAPLTAVGLVISFTGQSVITIVP
                     LTIAVLFATAYDYFIRKMRSLYVNPY"
     gene            2923..3633
                     /gene="deoD"
                     /locus_tag="ACUBV3_001499"
     CDS             2923..3633
                     /gene="deoD"
                     /locus_tag="ACUBV3_001499"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HHU3847994.1"
                     /translation="MSIHIEAKQGEIADKILLPGDPLRAKFIAENFLEDAVCFNTVRN
                     MFGYTGTYKGHRVSIMGTGMGMPSISIYARELIVDYGVKTLIRVGTAGAINPDIHVRE
                     LVLAQAAATNSNIIRNDWPEFDFPQIADFKLLDKAYHIAKEMDITTHVGSVLSSDVFY
                     SNQPDRNMALGKLGVHAIEMEAAALYYLAAQHNVNALAMMTISDNLNNPEEDTSAEER
                     QTTFTDMMKVGLETLISE"
     gene            3642..4409
                     /locus_tag="ACUBV3_001500"
     CDS             3642..4409
                     /locus_tag="ACUBV3_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000129264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HHU3847995.1"
                     /translation="MTDFSTALKVLVEQYSYHNAFLLLRKHGPLNSDLLFLLEMMKER
                     RELNIDFLFAHQEQVVILQEKYNIKLLHNSYDLELLANYIMDLEAKVKNGLIIDFVRS
                     VSPILYRLFMILLAQEVPHLHDYIYNARDDHYDTWKFKELKESNHPVLLAFSERWHDS
                     RLTSKSLAECLQLTDLDEEVKSTIIQLRQFEKSVRNPLAHLIKPFDEQELYRTTQFSS
                     QAFLDQIIFLAKVIGVEYDTVNFHYDTVNKLIIKILE"
     gene            complement(4434..5357)
                     /locus_tag="ACUBV3_001501"
     CDS             complement(4434..5357)
                     /locus_tag="ACUBV3_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HHU3847996.1"
                     /translation="MRIQQLQYVIKIVETGSMNEAAKQLYITQPSLSNAVRNLETEMG
                     IQIFIRNPKGITLTKDGMEFLSYARQILEQTALLEERYKGDNTSRELFSVSSQHYAFV
                     VNAFVALFNGTDMTQYELFLRETRTWEIIDDVKNFRSEIGVLFLNSYNRDVLTKLFDD
                     NSLIATTLFTTTPHIFVSKSNPLANRKKLNMKDLEDYPYLSYDQGLHNSFYFSEEMMS
                     QIPHPKSIVVSDRATLFNLMIGLDGYTVATGILNSKLNGDEIVAIPLDVDDVIDIVYI
                     RHDKANLSKMGQKFIDYLLEEVSFNHKNKQS"
     gene            5547..7004
                     /locus_tag="ACUBV3_001502"
     CDS             5547..7004
                     /locus_tag="ACUBV3_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HHU3847997.1"
                     /translation="MSNHSRRQQKKHSHTPLRVINLFLLVIFILLSVVSLFLMYRHHF
                     LAFRHLNVIYGVVIVLIILASLFLCIKNKARIFTTIILVLASIFVATTLYGFKSTIDL
                     TNNLNKTASYSEIEMSVVVPKDSKITNIEAVSKLAAPVKNDTSNITDLIEHIKSEKGI
                     SITPQKTDSYQDAYNRIKNGDSQAMVLNNAYVSLIELSTPDFKSQIKTIYTYKIKKKI
                     NRKNTNHKEGVFNIYISGIDTFGSISTVSRSDVNIIMTVNTNTHKVLLTTTPRDAYVK
                     IPDGGGNQYDKLTHAGLYGVETSMKTLENLYDINLDYYARINFSSFLKLIDLLGGVTV
                     YNDQAFTSKHGNFDFPVGQVTLNSEQALGFVRERYSLQGGDNDRGRNQEKVIAAIINK
                     LASSQSVTKLNSITSQLQTSVQTNMTIDNINDLINNQLSTGQRFTVESQALTGHGSTG
                     ELPSYAMPGAQLYMMSIDQSSLSNAKSKIKNTMEE"
     gene            7010..7741
                     /gene="cps4B"
                     /locus_tag="ACUBV3_001503"
     CDS             7010..7741
                     /gene="cps4B"
                     /locus_tag="ACUBV3_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     Cps4B"
                     /protein_id="HHU3847998.1"
                     /translation="MIDIHSHIVFDVDDGPKTLEESLSLIEESYRQGVRIIVSTSHRR
                     KGMFETPEDIIFKNFSIVKHEAEKRFEHLQILYGGELYYTSDMLEKLKLKQIPTLNNT
                     KFALIEFSMQTSWKDIHTALSNVLMLGITPVVAHIERYNALENQKERVKEIINMGCYT
                     QINSSHILKQKLFNDKHKRFKKRARYFLEENLVHFVASDMHNLDVRPPFLAEAYKIIC
                     RDFGKERANQLFIENAQSILKNHYI"
     gene            7750..8442
                     /locus_tag="ACUBV3_001504"
     CDS             7750..8442
                     /locus_tag="ACUBV3_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HHU3847999.1"
                     /translation="MNKIANTEVEINIFNLLKKLWKKKFLITFVAIAFATAGLFYSLF
                     IVTPQYTSSTRIYVINPNTPNNSITAQDLQAGSFLANDYKEIITSTDVLEKVISSEKL
                     NYPSSQLLQKITISILKDTRVISISVEDANPKMSQKLANSVREAAVSKIKAVTQVEDI
                     TTLEKGNLPKAPSSPNIKKNVLIGFIVGAGLSTIVLVIMGILDDRVNTEEDIEKVLGL
                     TSLGIVPDLNKL"
     gene            8453..9151
                     /locus_tag="ACUBV3_001505"
     CDS             8453..9151
                     /locus_tag="ACUBV3_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000197400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HHU3848000.1"
                     /translation="MTRLEIVDSKLRQAKKTEEYFNAIRTNIQFSGKENKILAITSVR
                     EGEGKSTTSISLALSLAQAGFKTLLIDADTRNSVMSGTFKATGTIKGLTNYLSGNADL
                     GDIICETNVPRLMVVPSGKVPPNPTALLQNAYFNKMIEAIKNIFDYIIIDTPPIGLVV
                     DAAIIANACDGFILVTQAGRIKRNYVEKAKEQMEQSGSKFLGIILNKVNESVATYGDY
                     GYYGNYGKRDRKRK"
BASE COUNT         3101 a         1474 c         1703 g         3062 t
ORIGIN      
        1 atggggttat cagagtttga aaaaggcttc tcctttacta atttggttga ctttgatgct
       61 ctctatggtc accgtcgtga tcctcatggt tatcgtgatt gtcttcatga gtttgatgag
      121 cgtcttccag agatcgtttc agcgatgcgt gacaaagact tacttttgat cactgccgat
      181 catggtaatg acccaactta tgcaggtaca gaccatacaa gagaatacat tcctctctta
      241 gcctatagcc catctttcac aggaaacggc cttatcccag tagggcattt cgcagatatt
      301 tcagcaacag ttgcggataa ctttggtgtt gatactgcga tgattggaga gagcttcttg
      361 caggacttgg tataaatttt tatttcctta agacacttgt agcattaaaa attgagagga
      421 agatgatgga aaaagttagg atttaccata acccaaattg tgggacatca aggaacgtac
      481 ttgccattat tcgtcattgt ggtattgaac cagaaattat ttattatctg aaaacaccac
      541 ctagcaagat ggcactagtt gaactgttgt tagaaatgaa actttctgtg agagaacttc
      601 taagaacaga tgttcctgct tatgagaaat tcaatttgga atcttctagc gttacagatg
      661 aagaaatgat tgatgcgatg atacaagatc ctgttcttat caatcgtccc atagttgtta
      721 cgagtaaagg tgcaaaactc tgccgaccct gtgaagaaat tttaaccatt ttacctgtaa
      781 agatggaaaa agattttgtt aaagaagatg ggcagattat ccaaagttta tagcatattg
      841 tatagtgaat aatagtttag gaggtaagta aatgacatta ttagaaaaaa ttaatgagac
      901 tagagacttt ttgcaagcaa aaggcgtcac agcaccagaa tttggtctta ttttaggctc
      961 tggtttagga gaattagctg aagaaatcga aaatcccatt gttgtggatt atgcagacat
     1021 cccaaattgg ggacagtcaa cagtagttgg tcatgctgga aaattagtgt atggggattt
     1081 atcaggccgt aaggtattag cgcttcaagg tcgttttcac ttttatgaag gaaatacaat
     1141 ggaagtcgtt actttcccag tacgtatcat gagagcattg gcttgccaca gtgtgcttgt
     1201 gactaatgca gcgggtggga ttggatacgg accaggaact ttaatgctga tcaaagacca
     1261 catcaatatg attgggacta accctctcat aggtgagaac cttgaagaat ttggaccacg
     1321 tttcccagac atgtcggatg cttatacagc aacatatcga caaaaagctc accaaattgc
     1381 tgaaaaacag aatatcaaac tcgaagaagg tgtgtacttg ggtgtatcag gacccactta
     1441 tgaaacacct gcagaaattc gtgcattcca aacaatgggc gcacaagcgg taggtatgtc
     1501 cacggttcca gaggtgatcg ttgcagctca ctcagggctt aaagtgttag gaatttcagc
     1561 aattactaac tttgccgctg gcttccaatc agagctcaat catgaggagg tcgttgaagt
     1621 tactcagcgt attaaagaag atttcaaggg attagttaaa tcattagttg ctgaacttta
     1681 aaatggtttc tcgattatac tatgctgtca agtttatgat agccgttcta tttatgacag
     1741 ttatggcagg agttggagct atcttaatgc actatgtatt aatgtttaca gagtggttag
     1801 catttggtga tagtagagaa aatactctgt ctttgttaaa tagtgtcaca ccaattaaaa
     1861 gagtgttatc acttacttta gtctcctttt tagcatccct tagttggtat tacttacaaa
     1921 tcaagccaaa gcaaataact agtattaaac aacaggttgt ttttaaggat ttttctgtaa
     1981 aaaaatcacc ttactggcta catataggtc acgcattttt gcaattgatt tatgtaggag
     2041 ctggtggtcc tattggaaaa gaaggagcgc cacgagagtt tggagccata aatgctggga
     2101 aaatatcgga tttattagca cttaaagtgc tggataaacg tttattaatc gttagtggtg
     2161 cagctgcagg tttgtcagca gtttatcaag tacccttagc tagtgtattc tttgcttttg
     2221 aaactttagc tttaggaatt tcactgaaaa atattgtgac attgctagtc tctacttttg
     2281 gtgcagctag tattgctcaa ttagttattt cgacagcccc tctttatcat atttcaaaga
     2341 tgagtctgaa ttcacagtca ttagctttta tgttcttgat cgttttatgt gtaactccga
     2401 ttgcgattag ctttagatat ttaaatcaaa aagtgacaga gagacgtatt aaaaatataa
     2461 aaatattgtt aagtttgcca gtagtgtcat tgatagtatc tgttttatca ataatctatc
     2521 cccagatttt aggaaatggt aatgccctag ttcaagaagt ctttaaaggg accacagtta
     2581 gcttaatcgc tattttagtt gttctcaaga tgatagcaac attatccact ctttacgctg
     2641 gtgcttatgg cggtatactg acaccatctt tttccattgg tgcttgttta ggttttttgt
     2701 tagctagtat ttctattcct ttacttcccc acctatcgat agtaaccagt atgttggttg
     2761 gtgcagctat ttttttagct attacaatga gagcgccact gacagctgtc ggtttagtca
     2821 tatcttttac tggtcaatct gttataacta ttgttcccct aactatagca gtactttttg
     2881 ctactgccta tgattatttt ataagaaaaa tgaggagttt atatgtcaat ccatattgaa
     2941 gcaaaacaag gtgaaattgc agataaaatt ttacttccag gagatccact ccgtgcgaaa
     3001 tttattgccg agaatttttt agaagatgcg gtgtgtttta atacagttcg caatatgttt
     3061 ggttatacag gtacctataa aggacatcgt gtttctatta tgggaactgg tatgggtatg
     3121 ccttcaattt caatttatgc tcgcgaacta attgttgatt atggggttaa aacattgatt
     3181 cgcgtaggaa cagcaggtgc tattaatcct gatatccatg ttcgtgaact tgttttggca
     3241 caagcagcag caacaaattc taatattatc cgtaatgatt ggcctgaatt tgattttccg
     3301 caaattgctg attttaaact tttggataaa gcttatcaca tcgcaaagga aatggatatt
     3361 acaactcatg taggcagtgt attatcttca gacgtgtttt attcaaacca accagatcgt
     3421 aatatggctt tgggtaaact aggtgttcat gctattgaga tggaagcagc agctctctac
     3481 tatcttgcag ctcaacacaa tgttaatgct cttgctatga tgacgatttc agacaacttg
     3541 aataatccag aagaagatac aagtgcagaa gagcgtcaaa caacatttac tgatatgatg
     3601 aaagttgggc tcgaaacact tatttctgaa tgaggataga aatgacagat ttttcaactg
     3661 ctcttaaagt attagtggaa cagtattctt atcataatgc tttcttatta ttacgaaaac
     3721 atggtccttt aaactcagat ttattatttt tattagaaat gatgaaagag cgtcgagagt
     3781 taaacattga ttttcttttt gctcatcaag aacaagttgt tatattacaa gaaaaataca
     3841 atataaagct tttgcataac tcgtatgatt tagagttgct ggcaaattac atcatggatt
     3901 tagaagcaaa agtaaaaaat ggattaatca tagattttgt ccgtagtgtt agtcctattt
     3961 tatatcgtct atttatgatt ttacttgcac aagaagtacc tcacttgcat gattacatct
     4021 ataatgcaag agatgatcac tacgatactt ggaagtttaa agaattaaag gagtcaaacc
     4081 atccagtcct tttggcattc tctgaaaggt ggcacgatag tcgcttgact tctaaaagcc
     4141 ttgcagaatg tttacaatta accgaccttg atgaagaagt gaaatcgacc atcattcaat
     4201 taagacagtt cgaaaaatca gtcagaaatc ctttggctca cctgattaaa ccttttgatg
     4261 agcaagaact atatcgtaca actcaatttt cttctcaagc atttttagac cagattatct
     4321 tcttggcaaa ggtaattggt gttgagtatg atactgttaa ttttcactac gatacggtta
     4381 acaagcttat tataaagata cttgagtaat ttaaaaacag ttctgagaca atctcaggac
     4441 tgtttatttt tatgattaaa cgaaacttcc tctaataaat agtcaataaa tttttgtccc
     4501 atttttgaaa ggtttgcttt gtcgtgtcgg atatagacaa tatcaataac atcatccacg
     4561 tcaagaggga ttgcgacaat ttcgtcaccg ttcaattttg agttgaggat accagttgcc
     4621 actgtataac catctaatcc aatcattaaa ttaaacagag ttgctctatc actgacaaca
     4681 atggattttg ggtgaggaat ttgagacatc atttcctcag aaaagtagaa agaattatgc
     4741 aggccttgat cataactcag ataaggataa tcttctaaat ctttcatatt taattttttt
     4801 ctattagcta agggattgga tttgctcacg aaaatatgag gtgtagtagt aaaaagagtt
     4861 gtggcaatta aggaattatc gtcgaataat ttggttaata catctctatt atagctatta
     4921 agaaacagaa caccaatctc tgaacggaaa tttttaacat catcaataat ttcccaagtc
     4981 ctagtttcac gtaaaaaaag ttcgtattgg gtcatgtctg tgccattaaa gagagctaca
     5041 aaagcgttaa ccacaaatgc atagtgttgt gaagaaacgc tgaacagctc acgacttgta
     5101 ttatcaccct tataacgctc ttcaagaagt gctgtttgtt ctagaatttg tcgcgcatat
     5161 gacagaaatt ccataccatc ttttgttaac gttattcctt ttggatttcg aataaaaatt
     5221 tggataccca tctcagtctc aaggttccta acagcgtttg aaagtgaagg ttgggtaatg
     5281 tagagttgtt tagcggcttc attcatgcta ccagtttcta caatcttaat aacatattgt
     5341 aattgttgaa ttctcataac tctagtttaa actagaatgt aaataaattc aattagatca
     5401 gctaaattat caatatagat caattgaaaa tggtatttag ctaatataat cagattattc
     5461 caatattact ttcgttgaat attgtattga aaaatgatat aatatccaag taaaattaag
     5521 gtttttttaa gtattggaga attacaatgt ctaatcattc gcgccgtcaa caaaagaaac
     5581 actcacatac acctctacgg gtgattaatt tatttctttt ggtgattttt attttgttaa
     5641 gtgtagtctc attatttctt atgtatcgtc accatttttt ggcatttaga cacttgaacg
     5701 tcatttatgg agttgtaatt gttttaatca ttttagcaag tttatttctt tgtattaaga
     5761 ataaagctag aatttttaca actataattt tagtactagc ttctattttc gttgctacta
     5821 ctttatatgg atttaagtca accattgatt tgacaaataa tctaaataaa actgcttcat
     5881 actctgaaat tgagatgagt gtagttgtac caaaagattc taagataacc aatatagaag
     5941 ctgtcagcaa attagccgca ccagttaaaa acgatacttc aaatattact gatttgatag
     6001 aacatataaa atcagaaaaa ggaatctcta ttacaccaca aaaaacagat tcttaccagg
     6061 atgcatacaa tagaattaaa aatggtgata gtcaggctat ggttttaaat aatgcttatg
     6121 ttagcttaat tgaacttagc acccctgatt ttaaatcgca gataaaaacg atttatactt
     6181 acaaaattaa gaaaaaaatt aatcgtaaaa atactaatca taaagaaggg gtatttaata
     6241 tctatattag cggtattgat acttttggct ctatatcaac agtatcaaga tctgatgtaa
     6301 atattattat gacggttaat accaataccc acaaagtatt gttaacgaca acaccacgag
     6361 atgcctatgt aaaaattcca gatggtgggg gcaatcaata tgataaatta acccatgcag
     6421 gtttgtatgg cgttgagaca tcaatgaaaa cacttgaaaa cctttacgac atcaaccttg
     6481 attattatgc tagaattaat ttttcatcat ttttaaaatt aatagacctc ttgggaggag
     6541 tgacagttta taacgatcaa gcttttacaa gtaaacatgg taattttgac ttccctgttg
     6601 gtcaagtaac attgaattct gagcaggctt tgggctttgt tagagaacgt tattctctac
     6661 aaggaggcga taacgataga ggtagaaatc aagaaaaagt gattgcagct attataaata
     6721 agttagcttc tagtcagtca gtaacaaaat taaatagcat tacctcacag ctccaaacgt
     6781 ccgttcaaac taatatgact attgataata ttaatgattt gattaacaat caattgtcaa
     6841 ctggacaacg cttcactgtc gagtcacaag cattaactgg tcatggttca acgggcgaac
     6901 ttccttcata tgcaatgcca ggagctcaac tttatatgat gtcaattgat caatctagct
     6961 tatctaatgc aaaatcaaaa attaagaaca caatggagga ataacaagaa tgattgatat
     7021 tcattctcat atcgtttttg atgtagatga tgggcccaaa acgcttgaag agagtttatc
     7081 tttaatagaa gaaagttatc gacaaggtgt tagaataatt gtttcgacat cacatagaag
     7141 aaaaggtatg tttgaaacac ctgaagatat tatcttcaaa aacttttcta tagttaaaca
     7201 cgaagcggaa aaaagatttg aacatcttca aatcttatat ggtggagaat tgtactatac
     7261 aagtgatatg ttagaaaaac tgaagttaaa gcaaattcca actttaaaca atacgaaatt
     7321 tgctttaatt gaattttcta tgcaaacttc ttggaaagat attcatacag ctttgtcaaa
     7381 tgttttaatg cttggtatta caccagtcgt tgcgcatata gagaggtata acgctttaga
     7441 gaatcaaaaa gaacgggtga aggaaattat taatatgggg tgttacacac aaataaatag
     7501 ttcccatatt ttgaaacaaa aactttttaa tgataagcat aaacgcttta agaaaagagc
     7561 ccgttatttt ttagaggaaa atttagtgca ttttgtagcg agtgatatgc ataaccttga
     7621 tgttagaccg ccatttttag cagaagctta taagattatc tgtagagatt tcggtaaaga
     7681 acgtgctaac caacttttta ttgaaaatgc tcaatctata ttaaaaaacc attacattta
     7741 ggagatttca tgaataaaat agctaataca gaagttgaaa taaatatatt caacctttta
     7801 aaaaaattgt ggaaaaagaa atttttaatc acatttgttg ccattgcatt tgcaacagct
     7861 ggacttttct atagtctttt tattgttacg ccacaatata cttcttcaac aaggatatat
     7921 gttattaacc ctaatacccc taataatagt attactgcac aagatctaca agcggggagt
     7981 tttcttgcaa atgactataa ggagattatt acgtctactg acgttctaga aaaagttatt
     8041 tcttctgaaa agttgaatta tccttcgtct cagttgctac aaaaaataac aatttctatt
     8101 ttaaaagata cacgtgttat ttcaatatcg gtcgaagatg ctaatccaaa aatgtctcaa
     8161 aaattagcaa attcagttag agaagcagca gtttcaaaaa tcaaggcagt tactcaagta
     8221 gaggatatca ctactcttga gaagggaaat ttacctaaag caccatcttc tcctaatatt
     8281 aaaaagaatg tactaatcgg gtttattgtt ggtgcaggat tatcaactat tgttttagtt
     8341 attatgggta ttttggatga tcgtgttaat actgaagaag atattgaaaa ggtcctagga
     8401 cttacttctt taggtatagt accagatttg aataaacttt aaggagaata taatgactcg
     8461 tttagaaata gttgatagca agttaagaca agcaaaaaaa acagaagaat acttcaatgc
     8521 gatccgtaca aacatacagt ttagtggaaa ggaaaataaa attcttgcaa ttacctctgt
     8581 tagggaaggg gaaggaaaat ccactacttc aataagttta gctttatctt tagctcaagc
     8641 aggatttaaa acattattaa tagatgcgga tactaggaac tctgttatgt ctggaacctt
     8701 taaagcaact ggaactatta aaggcttgac gaattattta tcaggtaatg cagatcttgg
     8761 agatattatc tgtgaaacca atgttcctag actgatggtc gttccttcag ggaaagtacc
     8821 accaaatcca acagcattac ttcagaacgc ttattttaat aagatgattg aagctattaa
     8881 aaatatattt gattatatta tcatcgatac tccacctatt ggtttagttg ttgatgccgc
     8941 aataatcgct aatgcttgtg atggttttat tttagtaacc caagcaggta gaataaaacg
     9001 taattatgtt gaaaaagcaa aagaacagat ggaacaaagt ggttcaaagt tcttaggtat
     9061 tattcttaat aaagttaatg aatctgttgc tacttacggc gattacggct attatggaaa
     9121 ttacggaaaa agggatagaa aaaggaagta aggggctctt gtattgaaag aaaaagaaaa
     9181 tatacaaaag attattatag cgatgattca aaccgttgtg gtttattttt ctgcaagttt
     9241 gacattaaca ttaattactc ccaactttaa aagcaataaa gatttattgt ttgttctatt
     9301 gatacattat attgtctttt atctttctga tttttacaga
//
LOCUS       DBJYSH010000081         9248 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.081, whole genome shotgun sequence.
ACCESSION   DBJYSH010000081 DBJYSH010000000
VERSION     DBJYSH010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 9248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9248
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.081"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            complement(<1..483)
                     /locus_tag="ACUBV3_001506"
     CDS             complement(<1..483)
                     /locus_tag="ACUBV3_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HHU3848001.1"
                     /translation="MSRSQKVILTNMCLIEDNKGRIVMQIRDPKRYSWSGAALPGGHI
                     EEGESLYDALVREVREETGLTISNPKLVGMKHWYTDEGIRYLVFLYKTYDFSGELQSS
                     DEGDVIWIERSQLDKIELAYDMLNLLRVFDEDDISEMFYSKRLEDGFIRKILLNKKEL
                     G"
     gene            complement(635..1696)
                     /locus_tag="ACUBV3_001507"
     CDS             complement(635..1696)
                     /locus_tag="ACUBV3_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HHU3848002.1"
                     /translation="MDTPKLTINEADLLSGKVKVVIAKAEDNHLKNLTITVNGKPYTE
                     GQSIDSYQLKGFEGRYVIKVSAVDKSGKVTEKIITIGNFPELSASLRKTSFQMQDLTS
                     IDNLLSLAHNQTAELIKPVQLMDKKVSFTAKISDPLGRSVVREFEALLSTSNLKVDSK
                     LKPHLKKQTLLLTDPKGITDLFDLDKDSSILLTEIVDNKVGNHTIKGLIFKNGAYNHF
                     ETSIQIVKTMEEINSDRRLKNDHKSSDDTKLKKETESLKGHKVDPAVHGEFKEISKHQ
                     MDKPQVIAVGMKGSTKTPPFVHSQMEAKKTTKIPMSRAAKHELPRTSDDNNNLLAKGI
                     ALIVIVLGLLGLKKASKKY"
     gene            complement(1802..5959)
                     /locus_tag="ACUBV3_001508"
     CDS             complement(1802..5959)
                     /locus_tag="ACUBV3_001508"
                     /inference="COORDINATES: protein motif:HMM:NF012311.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HHU3848003.1"
                     /translation="MKRISLKTTGVLIAFLAVNAVATSVVVDADTVVPHTSANTSQLL
                     EKNTTIKPSEVKQSRTESNAVTPPTIASSNSQKLENKSSVDSNILKSEQPAHKVAGVK
                     QVVQEQTKNQKDETSLQAKKQVQSSEKLLNTSSQPSSEENKNTNKPNKANEVDMKNVV
                     NNASTKPTPPSDSKVYKRVIVTFDQVNKEKVDEALKAIPGTRIKLTYSSLFNGYSLAV
                     DEKEIEKIGKIKEIKHIEAVQVLKPQMFTANQWTHVLAARLKAKQNSKVDGRGLLIAT
                     IDSGVDIHHPAMRLDENDPDVMAKRRIKDIKAPYTNKVPFAYNYQRGDNLELKDDVQR
                     PHGMHLAGILAGNAKDGFKGVAPNAQLLVYKISTNREDPSAEWTGSNAVFHAMEDAAK
                     RGADVISLSFGYLGTGRPGELWYETIKKVTDKGVIVVAAMGNYGNSASSTTYDNVVDN
                     SLGTKDTAAMVSVAAVDKVIGVGSVQNSALLLDVLKVGNLKLGYAELGHKSQTILKML
                     KNKTFDLVYVGKGQLKKDISEEDLKSYTGKVIVVERGGEGVKDKLKRFIGIAKGVIMI
                     NEPAYYSSGNFDTHPMFDYHYDIPEGKWAISLSKKDGDQLIDYIKKNKTAKLEFLPKE
                     TFVRRPNPTISGFSSWGVTPDLHLKPDVVAPGEDIYSTFNDGTYGYMSGTSMATPQVA
                     AISALVKSKVAEIMALKLPMLAGLTNVDLTKIVLMNTSQPLKDTLNKALEISPRRQGA
                     GMVNLEKALANDVLVFGDKTGSFSIKEIKNHKRFTLKLVNTGQKRRNFSIEPGAILTT
                     KIVDKVTNQGYENVVLKAVHAKVIEGATLQMGKVISLAPQSAIELDIDLEIKKPIQDF
                     AEGYLYFKTLDKDGQDISAPYMGFSGTWDLEPIFDAPMWDSRSKNKLTTLMQATLVGH
                     GTNKFYEIGQEIGSKKVNPSLFTMSNVRVAPDKVVRVAPRFSLLRSASDFDLSIVSEA
                     KDDSPILRRITVKHFVEKYYKSRNEEEDSYYDALRTTESSLVWDGKFYDPKTGDFKAA
                     KPGLYYFKLRARSNPTDPYQVQYLPILIDNERPTFSLKKVNNGFDILTHDNHRVKEVI
                     VRNKETQKPITVKKIAENHYHIDELTLRESDLDIKVADYAGNSNKEIALSESEEVSEK
                     SYSSEEDNTLQPQSLSHRNMKAQATSSDDDDIWDDNDDDLNEEKDFDFEENISDIEDG
                     LLLTRETVYSIRTVNKIRNVENESLEHSVSLLLKDGYRAEIRVVNLHENAKDPKHSDP
                     YRTVSNPIYMYGDDSDTFKTTWVKFMSGNNIMNLKVYNKNDKLVFSRGYHITVDVQPP
                     KLELDKTNIEEAADKDNFITGQIYAQNGEIEIKGHVSDNVKGWGLSINGDSVDVKYSS
                     SEFISNR"
     gene            complement(6202..8193)
                     /gene="uvrB"
                     /locus_tag="ACUBV3_001509"
     CDS             complement(6202..8193)
                     /gene="uvrB"
                     /locus_tag="ACUBV3_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HHU3848004.1"
                     /translation="MIDRKDTNRFKLVSKYSPSGDQPQAIETLVDNIEGGEKAQILKG
                     ATGTGKTYTMSQVIAQVNKPTLVIAHNKTLAGQLYGEFKEFFPDNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVVASVSCIYGLGSPKEYA
                     DSVVSLRPGQEISRDQLLNNLVDIQFERNDIDFQRGKFRVRGDVVEFFPASRDEHAFR
                     IEFFGDEIDRIREIESLTGRVLGEVEHLAIFPATHFMTNDEHMEEAISKIQAEMENQV
                     ELFEKEGKLIEAQRIRQRTEYDIEMLREMGYTNGVENYSRHMDGRSEGEPPFTLLDFF
                     PEDFLIMIDESHMTMGQIKGMYNGDRSRKEMLVNYGFRLPSALDNRPLRREEFESHVH
                     QIVYVSATPGDYEMEQTDTVVEQIIRPTGLLDPEVEVRPSMGQMDDLLGEINLRTEKG
                     ERTFITTLTKRMAEDLTDYLKEMGVKVKYMHSDIKTLERTEIIRDLRLGVFDVLIGIN
                     LLREGIDVPEVSLVAILDADKEGFLRNERGLIQTIGRAARNSNGHVIMYADKITDSMQ
                     RAMDETARRRRLQMDYNEKHGIVPQTIKKEIRDLIAITKSNDSDKPEKVVDYSSLSKK
                     ERQAKIKALQKQMQDAAELLDFELAAQIRDVILELKAID"
     gene            complement(8254..9174)
                     /locus_tag="ACUBV3_001510"
     CDS             complement(8254..9174)
                     /locus_tag="ACUBV3_001510"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HHU3848005.1"
                     /translation="MNKRILNSQFAVYKNWPSWLLLLFACFMVYLFFFVGLTLGGVGI
                     VLVSSVLYKFSSYTFLEFINLFNNIYGELGTFSFSAFLLLIWVKFVEKRPFSELGFST
                     KFKRTLWSLIKGWSIGFILFSISVITAYILGGLDFHSYDVSKATIFYVVTLLPFWLIQ
                     SGTEELLTRGWLLPLINHRFHLAVAIGVSSTLFGILHLVNAHVTFLSIVSIICSGVLM
                     SLYMIKSGNIWSVAALHGAWNFSQGNLYGIAVSGQKAGASLLHFTVKENAPDWISGGA
                     FGIEGSLISIFVLLAAIIYLLWLIKTEETD"
BASE COUNT         2796 a         1810 c         1433 g         3209 t
ORIGIN      
        1 tcctagttct tttttgttta ataaaatttt tcttataaaa ccatcttcaa gtcgcttact
       61 ataaaacatc tctgagatgt catcttcgtc aaatactctc aaaaggttaa gcatatcata
      121 tgctagttca atcttatcaa gttgtgaacg ttcaatccat ataacatcac cttcatctga
      181 cgattgtaat tcacctgaaa aatcatatgt tttataaagg aataccaaat aacggatgcc
      241 ttcatctgtg taccaatgct tcataccaac taatttggga ttacttattg ttaaacctgt
      301 ctcctcgcga acttctcgaa cgagagcatc atacaagctt tctccttctt caatatggcc
      361 gcctggtaga gcagctcctg accaagaata acgtttagga tcacggattt gcatcacaat
      421 acgtccttta ttatcttcaa taagacacat attagttaaa ataacttttt gactgcgtga
      481 cattatttcc tctagtatat aaatttagtg atagagttac aataccattt atttatcaat
      541 ttttctacaa aataatagat taagttaaaa ctcccttaat aatttatcca aaggaatttt
      601 agcttaataa attagaaaac taatttaatc aaaatcaata tttctttgaa gcttttttaa
      661 ggcctagtaa accaagtaca attactatga gtgcaattcc tttagccagt aaattattat
      721 tgtcatcact tgtccttggc aattcatgtt ttgccgctct agacattgga atcttagttg
      781 ttttcttcgc ttccatttga ctatgaacaa atgggggtgt ctttgtactc cctttcatgc
      841 ctacagctat cacttgtggt ttatccattt gatgtttaga tatttcttta aattcaccat
      901 gaactgctgg atcaacctta tgccctttta acgactctgt ttcttttttt agttttgtgt
      961 cgtcagagct tttatgatca tttttcaaac gtctgtcaga gttgatttct tccattgttt
     1021 ttacaatttg tatacttgtt tcgaaatggt tatatgcacc atttttaaat atcaaaccct
     1081 ttatagtatg atttcccact ttattatcga ctatttctgt caataaaata gagctatcct
     1141 tatccaagtc gaaaaggtcc gtaattcctt ttggatctgt taaaaggaga gtttgttttt
     1201 tcaaatgtgg ttttagtttt gaatctactt taaggtttga agttgataat aatgcttcaa
     1261 actctctaac cacagaacga ccaagggggt cagatatttt tgccgtaaag ctcacttttt
     1321 tgtccatgag ttggactggc ttaatcaatt ccgcagtctg attgtgggct agggataata
     1381 aattatcgat agaggtaaga tcttgcattt ggaaagaggt tttgcgtaaa cttgctgata
     1441 attcagggaa gttgccgatt gttataattt tttcagtgac tttacctgat ttatcaactg
     1501 ctgatacttt gatgacgtaa cgcccttcga aaccttttaa ttggtaagag tcaatacttt
     1561 gtccttctgt gtagggcttg ccattaacag ttatcgttaa atttttaagg tgattatctt
     1621 ctgctttagc tataaccact ttgacttttc cggataataa atctgcttca ttaatagtta
     1681 acttcggtgt atccacatca ttaactacct tataatgttc agaaacggta ttacctgatg
     1741 tatctaccgc tgaaatcgta acaatatctc ctgctgctgc ttttatctta tgagtaaatg
     1801 ctcatctatt actgataaat tcactacttg aatatttaac atcgacgcta tcaccattaa
     1861 tagaaagacc ccaacctttt acattatcag aaacgtgccc tttgatttca atttctccat
     1921 tttgagcata gatttgacca gtgataaagt tatctttgtc agcagcttct tctatgttag
     1981 tcttgtccag ttctaatttt ggaggttgta catcaacggt aatatgatag ccacgactaa
     2041 ataccaattt atcattctta ttataaacct tcaaattcat gatgttattc ccagacataa
     2101 atttgaccca tgtggtttta aatgtgtcag agtcatcacc atacatataa atagggttgg
     2161 atactgtacg atatgggtcg gaatgttttg gatctttagc gttctcatga aggttaacaa
     2221 ctcttatttc tgcacgatag ccatctttta ataagagaga aacactatgc tccaagcttt
     2281 cattttcaac atttcgaatt ttatttacag ttcgaattga atataccgtt tctcgagtta
     2341 ataataaacc atcctcgata tcagaaatat tttcttcaaa atcaaaatct ttttcttcgt
     2401 taaggtcatc atcgttatca tcccagatat cgtcatcatc tgaactggtt gcttgagctt
     2461 tcatattgcg atgagacagt gactgtggtt gtaatgtatt atcttcctca ctagagtaag
     2521 atttctctga tacttcttct gattctgata aagcaatttc cttgtttgaa ttcccagcat
     2581 agtctgcaac ttttatatcc agatcagatt ctcttaacgt taactcatca atatgatagt
     2641 gattttcggc aattttttta actgtgatag gcttttgtgt ctctttatta cgaacaatta
     2701 cttctttaac acgatgatta tcatgggtta aaatatcaaa gccgttatta acttttttaa
     2761 gagaaaaagt tggtctttca ttatctatta aaattggaag gtattggact tgataaggat
     2821 ctgtaggatt tgaacgtgca cgtaacttaa agtaatataa gcctggcttt gcagctttaa
     2881 aatcaccagt cttaggatca taaaactttc catcccaaac tagcgatgat tctgtcgttc
     2941 tcaaagcatc ataatatgag tcttcttcct catttcttga tttgtaatat ttctccacga
     3001 aatgtttcac tgttatacga cgaagtattg gtgaatcgtc ttttgcttct gatacaatag
     3061 agaggtcaaa atctgatgct gagcgtaata aagaaaatcg aggtgctaca cgcacaactt
     3121 tatcgggagc tacacgcaca ttagacatag taaataggga tggattgacc tttttactac
     3181 ctatttcttg tccgatttca taaaatttgt tagttccgtg tccaaccaaa gttgcttgca
     3241 tcaaggtcgt taatttattt ttagaacgac tatcccacat aggtgcgtca aaaattggtt
     3301 ctaaatccca tgttcctgag aagcccatgt aaggagctga gatatcttgt ccatctttat
     3361 ctaaagtttt gaaatacaaa tacccttcag caaaatcttg tataggtttt tttatctcaa
     3421 gatctatatc aagctctatg gctgattgcg gtgctagtga gataacttta cccatttgta
     3481 aagttgctcc ctctattact ttagcgtgaa ctgcctttaa aacgacattt tcatacccct
     3541 gattagtcac tttatcaaca attttagttg ttaaaatcgc tcctggttca atactaaagt
     3601 tacgacgttt ttgtcctgta ttgaccaatt tcaaggtaaa tcttttatgg ttcttaatct
     3661 cttttataga gaatgatcct gttttatcac caaaaacaag aacatcgtta gctaaagctt
     3721 tttctaagtt aaccatacct gcaccttgac gtcgtggtga aatttctaaa gctttattta
     3781 gagtatcttt caaaggttga cttgtattca tcaagacaat ctttgtcaaa tcaacgtttg
     3841 tcaaaccagc taacattgga agttttaatg ccattatttc agctacttta ctttttacta
     3901 atgcactaat ggcagctact tgaggggtag ccattgaagt tccagacatg tacccatagg
     3961 taccgtcgtt aaaagtagaa taaatatctt ctcctggagc aactacatct ggcttcaaat
     4021 gcaaatctgg tgtcactccc caactagaaa agcctgaaat tgtaggattt ggacggcgaa
     4081 caaaggtttc tttaggtaaa aattctaatt tagcagtttt gtttttcttg atgtagtcaa
     4141 tcaactgatc accatctttt tttgatagac taatagccca tttaccttct ggtatatcat
     4201 agtgataatc aaacatgggg tgagtatcaa aattcccaga actataataa gcaggttcat
     4261 ttatcataat aaccccttta gcaattccga taaaacgttt tagcttatct ttaacgccct
     4321 ctcctccacg ctcaacgacg ataacttttc cagtataact tttaaggtcc tcttctgaga
     4381 tatctttttt caactggcct ttaccaacat agactagatc aaatgtttta tttttgagca
     4441 tttttaatat tgtttgactt ttatgtccca gttctgcata tcctaattta agatttccaa
     4501 ctttcaaaac atctaaaaga agagcactat tttgaactga accaactcca ataaccttat
     4561 caacagctgc aacagacacc atagcagcag tatcttttgt tcccaaagag ttgtcaacga
     4621 cattatcgta ggtagtagat gaagccgaat taccatagtt tcccattgct gctactacga
     4681 taacaccctt gtcagtcacc ttcttaattg tctcatacca aagttcacct ggacgtcctg
     4741 ttcccaaata tccaaagctt aaactaataa cgtctgcccc tcttttagcg gcatcttcca
     4801 ttgcatggaa gactgcatta gagccggtcc actctgcaga tggatcttct ctatttgttg
     4861 aaattttgta cactaataac tgtgcattag gggcaactcc tttaaaacca tcctttgcat
     4921 ttcccgctaa gatacctgct aaatgcatac catgagggcg ctgaacgtcg tctttcaatt
     4981 ctaaattatc gcctcgttga taattgtatg caaaaggaac tttatttgta taaggagcct
     5041 taatatcttt aatacgacgc tttgccatca catctggatc attctcatcc aatcgcatgg
     5101 caggatgatg gatatctaca ccactatcaa tagtcgcaat aagtagccct ctaccgtcaa
     5161 ctttactatt ttgtttagct tttaaacgtg cagctaagac atgtgtccat tggttagcag
     5221 taaacatttg aggttttaat acttgaactg cttcaatatg ttttatctct ttaatcttac
     5281 caattttttc aatttctttt tcatctactg ctaatgaata gccattaaaa aggctactat
     5341 aagttaactt aatacgagtg cccggaattg ctttaagggc ttcatctact ttttccttat
     5401 ttacttgatc aaatgttaca ataacgcgct tatacacttt agaatcagat gggggagttg
     5461 gcttcgtcga agcattattc acgacatttt tcatatccac ttcattagcc ttattaggct
     5521 tattagtgtt tttgttttcc tcacttgaag gttgtgatga agtatttaat aacttttcac
     5581 ttgattgtac ttgcttttta gcctgtaaag aggtttcatc tttttgattt ttcgtttgtt
     5641 cttgaacgac ttgtttaaca ccagcaactt tatgtgcagg ttgttcactc tttagtatat
     5701 ttgaatctac agatgattta ttctcaagct tttgtgagtt actcgaggct attgttggag
     5761 gggttactgc attactttca gtacgtgact gtttaacttc ggaaggcttg atagttgtgt
     5821 ttttttctaa aagctgacta gtattcgcac tagtatgagg gactactgtg tctgcatcta
     5881 ccactacaga ggtcgctacc gcatttacag ctaaaaacgc aatcagtaca ccggttgttt
     5941 ttaaagaaat tcttttcata attccactca tttttaagtt agattatttt atttgttaag
     6001 cgagcttaat ttaagcactt ttgtatagag ttgtcaatat ctatttgcta attaatgagt
     6061 attttttaag aatttttatc ttatttagat tagttctttt taaccaaact aaaaagctaa
     6121 ctctacctgc tagagtcagc ttatttaata atctataacc ttctgctatc tcctaaagag
     6181 agaaccagat gagagataag tttaatcgat tgcttttagt tctaagataa catctcggat
     6241 ttgtgcagct agctcaaagt ccaataattc agctgcatct tgcatttgct tttgaagagc
     6301 tttaatttta gcttgccttt cttttttaga taaactactg taatcgacaa ctttttcagg
     6361 cttatcgcta tcattacttt tagtaatagc aattaaatca cggatttctt tttttattgt
     6421 ttgtggcaca attccatgct tttcattata atccatttgt aaacgacgac ggcgagcggt
     6481 ctcatccata gctctttgca tagaatctgt tattttatca gcatacataa taacatgacc
     6541 attactgtta cgtgctgcac gtccgattgt ttggataaga ccacgctcat tacgaaggaa
     6601 accttcctta tcagcatcta agattgcaac aagggaaact tcaggaacat ctattccttc
     6661 acgtaagaga ttaataccaa ttaaaacatc aaaaacacca agtcgtagat cacgaataat
     6721 ctctgtacgt tctaaggttt taatatcgct gtgcatatac ttaactttaa ctcccatttc
     6781 tttgagataa tctgttaagt cttctgccat acgctttgtc aatgttgtaa taaaggtacg
     6841 ctcacctttt tcagtcctca aattgatttc accaagcaag tcatccatct gccccatact
     6901 tggtctaact tcaacttctg gatcaagaag tcctgttggc cggataattt gctcaacaac
     6961 tgtatctgtt tgctccattt catagtctcc tggcgttgca gatacataga caatttgatg
     7021 gacatggctt tcgaattctt cacgacgaag tggacggtta tctaaggcag atggtaaacg
     7081 aaaaccgtaa ttaaccaaca tttctttacg cgaacggtct ccattataca tccctttaat
     7141 ttgccccatt gtcatgtgac tctcatcaat cataatcaaa aaatcttctg ggaagaaatc
     7201 taacaaagta aaaggtggct caccttcact tctaccatcc atgtgacgtg agtagttctc
     7261 aacaccattt gtatatccca tttcacgaag catttcaata tcatattctg tcctttgtcg
     7321 aatacgttgc gcttcaatca atttcccttc tttttcaaaa agttctactt gattttccat
     7381 ttcagcttgt atttttgaaa tagcttcttc catatgttca tcatttgtca taaagtgtgt
     7441 tgctgggaaa atagctaaat gttccacttc tcctagcaca cgccctgtta acgattctat
     7501 ttctcttata cgatcaattt catcaccaaa aaattcaatt cgaaaggcat gctcatcacg
     7561 actcgcagga aaaaattcta caacatcgcc acgaacgcga aattttccac gttgaaaatc
     7621 aatatcgtta cgctcaaatt gaatatcaac gagattgttc aataactgat cacgtgaaat
     7681 ttcttgacct ggcctaaggc taacaacact gtctgcatac tctttaggag aacctaaacc
     7741 gtaaatacaa gaaacagatg ctacaacaat aacgtcattt ctttctaata acgaagaagt
     7801 cgctgagtga cgtaatttgt caatttcgtc attgactgag ctatcttttt caatataagt
     7861 atccgaagaa ggcacataag cttctggttg ataataatca taatatgaaa caaagtactc
     7921 tacggcattg tcaggaaaaa attctttaaa ctctccataa agttgacctg ctagggtttt
     7981 attatgtgca atcaccaagg ttggcttgtt aacttgggcg ataacttggc tcattgtgta
     8041 ggttttacca gtaccagtag caccttttaa aatctgagct ttttctcctc cttcaatatt
     8101 gtcaaccaac gtttcaatag cttgaggttg atctcctgaa ggactatatt tggaaactaa
     8161 tttaaatcgg ttagtatctt ttctatctat catttcttta cctcgattac tttattttaa
     8221 catagaaaaa agaaaaagac tgagctgcaa cactcaatct gtttcttcag tcttaattag
     8281 ccatagaagg tatataattg cagctaagag aacaaaaata gaaatcaaac ttccttctat
     8341 tccgaatgca ccacctgata tccagtccgg tgcgttttct ttaacggtaa aatgcaaaag
     8401 agatgctcct gctttctgac cacttactgc aataccatag agattacctt ggctaaaatt
     8461 ccaagctcca tgtaaggctg caacgctcca aatattacca cttttaatca tatacaagga
     8521 cattaaaact cccgaacata taatacttac aatagaaaga aaagtgacat gggcatttac
     8581 caagtgcaaa atgccaaata aagtggatga gacccctatg gcaacagcta aatgaaaacg
     8641 atgattaatt agaggaagaa gccaccctcg tgttagcaat tcttccgtac cgctttgaat
     8701 aagccaaaaa ggaaggagag ttacaacata gaaaatcgtt gccttagaaa catcatagct
     8761 atgaaagtca agtcctccca atatataagc agtgatgaca gaaattgaaa atagaataaa
     8821 acctatactc cagcctttta taagtgacca cagagttctt ttaaactttg tagaaaaacc
     8881 caattctgaa aaaggtcttt tttctacgaa ttttacccag ataagcaaaa ggaatgctga
     8941 aaaactaaag gttcctaatt ctccatagat attattaaat aaattaataa actccaaaaa
     9001 agtataggaa gaaaacttat agagtacaga agacactaga acaattccca caccacctag
     9061 agttagaccg acgaaaaaaa ataggtatac cataaaacaa gcaaataaca ataatagcca
     9121 tgacggccag tttttataga cagcgaactg actatttaaa atccttttat tcataataat
     9181 cctaaatcct ttttgtgtga tgacactaga acaattatat cataatacta actaaagata
     9241 tatataaa
//
LOCUS       DBJYSH010000082         9232 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.082, whole genome shotgun sequence.
ACCESSION   DBJYSH010000082 DBJYSH010000000
VERSION     DBJYSH010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 9232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9232
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.082"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            complement(<1..782)
                     /gene="gatD"
                     /locus_tag="ACUBV3_001511"
     CDS             complement(<1..782)
                     /gene="gatD"
                     /locus_tag="ACUBV3_001511"
                     /EC_number="6.3.5.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0140282 - carbon-nitrogen ligase activity
                     on lipid II [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II isoglutaminyl synthase subunit GatD"
                     /protein_id="HHU3848006.1"
                     /translation="MTYTSLKSPTTKDYKYTLNVAHLYGNLLNTYGDNGNILMMKYVG
                     EKLGCQMTFDIVSLEDRFDPNYYQMAFFGGGQDYEQAIVARDLPSKKEDINKFIQNNG
                     VVLAICGGFQLLGQYYIQANGERIEGIGVMGHYTLNQNNNRYIGDIKIHNDEFNETYY
                     GFENHQGRTFLSEDEKPLGTVIYGNGNNKEDGTEGVHYKNVFGSYFHGPILSRNANLA
                     YRLVATALRNKYGKEIVLPSYEEILSLEIPEEYGDVKSKADF"
     gene            complement(782..2125)
                     /gene="murT"
                     /locus_tag="ACUBV3_001512"
     CDS             complement(782..2125)
                     /gene="murT"
                     /locus_tag="ACUBV3_001512"
                     /EC_number="6.3.5.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_function: GO:0140282 - carbon-nitrogen ligase activity
                     on lipid II [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II isoglutaminyl synthase subunit MurT"
                     /protein_id="HHU3848007.1"
                     /translation="MKINTALGVAAGKSAHYLLSKMGRGSTYPGSLALKFDKDILDTI
                     AKDYEIVVVTGTNGKTLTTALTVGILKEAFGQVVTNPSGANMITGIVSTFLTAKKSKS
                     GKKIAVLEIDEASLPRITQYIKPSLFVFTNIFRDQMDRYGEIYTTYQMILDGAANAPQ
                     ATILANGDSPLFNSKSVTNPVQFYGFDTDKHEPRLAHYNTEGILCPKCQAILTYRLNT
                     YANLGDYTCPNCDFERPNLDYALTRLIHLTNTSSGFVIDGQQYNINVGGLYNIYNALA
                     AVSVAEYFGVEASQIKDGFDKSRAVFGRQETFTIGNKKCTLVLIKNPVGASQALDMIK
                     LAPYPFSLSVLLNANYADGIDTSWIWDANFETILTMNIPEIFAGGVRHSEIARRLRVT
                     GYDEKRIKQADKLQDIMAMIEQQETEHAYILATYTAMLEFREILANHNAIRKEMN"
     gene            2265..3116
                     /gene="cdaA"
                     /locus_tag="ACUBV3_001513"
     CDS             2265..3116
                     /gene="cdaA"
                     /locus_tag="ACUBV3_001513"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HHU3848008.1"
                     /translation="MDIFSAIDSKFWASIMENPWMILIHLMDIIIVAVLIYKFIKALA
                     GTKIMSLIQGVILFIIIRFVSEWIGLTTITFLMNQIVTYGVIAGVVIFAPEIRTGLEK
                     FGRTPQLFTQRSQLSSDEKLVDALVKAVAYMSPRKIGALISIERTQTLQEYIATGIPL
                     DADISSELLINIFIPNTPLHDGAVIVKDKKIATACSYLPLSESSSISKEFGTRHRAAI
                     GLSENSDALTVIVSEETGGISVALKGEFLHDLSKDSFEAILRTQLIQNQEENSKLAWY
                     NQLLRRK"
     gene            3119..4078
                     /locus_tag="ACUBV3_001514"
     CDS             3119..4078
                     /locus_tag="ACUBV3_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017770882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HHU3848009.1"
                     /translation="MKKFFTNKFWLGVVSLFLAILLFLTATATSMNHQDNSKIAGASE
                     TYTHTLTDVPIDIKYDSDDYFISGYSYGADVYMSSVNRVKLDSEINEDTRKFKVVADL
                     TNMKPGTHKVPLKVVNLPSGVNATVSPTTITVTMGKKKTKEFPVYGHVNDKQIKAGYA
                     VDKMSVDVSKVKVTSDESIIDRIDHVAANIPDDKVLDDDFNKTVTLQAVTADGTVLAS
                     IIHPSKATLSVKVKKLTKTVPINLIPVGQFSDSISKINYKLSQEKAVISGTKEALEAI
                     SVINAEVDISDVTKNTEKKINLSANNVSVDPAQVTVQLTTTKK"
     gene            4132..5484
                     /gene="glmM"
                     /locus_tag="ACUBV3_001515"
     CDS             4132..5484
                     /gene="glmM"
                     /locus_tag="ACUBV3_001515"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HHU3848010.1"
                     /translation="MGKYFGTDGVRGEANVELTPELAFKLGRFGGYVLSQHETDRPRV
                     FVARDTRISGEMLESALIAGLLSVGIEVYKLGVLATPGVSYLVRTEKASAGVMISASH
                     NPALDNGIKFFGSDGFKLDDDRELEIEALLDAKEDTLPRPSAQGLGTLVDYPEGLRKY
                     EKFMESTGIDLEGMNVALDTANGAATASARNIFLDLNADISVIGDQPDGLNINDGVGS
                     THPEQLQSLVRENGSDIGLAFDGDSDRLIAVDENGEIVDGDKIMFIIGKYLSDKGQLA
                     QNTIVTTVMSNLGFHKALDREGIHKAITAVGDRYVVEEMRKSGYNLGGEQSGHVIIMD
                     YNTTGDGQLTAIQLTKVMKETGKKLSELASEVTIYPQKLVNIRVENNMKDKAMEVPAI
                     AEIIAKMEEEMDGNGRILVRPSGTEPLLRVMAEAPTNEAVDYYVDTIADVVRTEIGLD
                     "
     gene            5607..5978
                     /locus_tag="ACUBV3_001516"
     CDS             5607..5978
                     /locus_tag="ACUBV3_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HHU3848011.1"
                     /translation="MTKEAELLWAKHKYLVLSKSQKIYLDIRQTLKSPNCTVLDVQSL
                     IDQAVLLEESPSQVTNAYMHIWGYFKNKAERQEKEEFLTLLEKYRKTGYQRRKLLAFL
                     KQLLAKYPNSYLQNSSIFEEE"
     gene            6003..6383
                     /locus_tag="ACUBV3_001517"
     CDS             6003..6383
                     /locus_tag="ACUBV3_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02328 family protein"
                     /protein_id="HHU3848012.1"
                     /translation="MRLWHQDIIELLPRQQLLGQHRECCALRGNGWGRKHETVDYVFR
                     YSPYRLFAYHQLVMEEMMERGYRVSKEWLIAEYRGMKCPRYDTLNPVDLETPIYPEHN
                     QDYLQECLWNLKAKGIDLPINKIK"
     gene            6476..7606
                     /gene="hemW"
                     /locus_tag="ACUBV3_001518"
     CDS             6476..7606
                     /gene="hemW"
                     /locus_tag="ACUBV3_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HHU3848013.1"
                     /translation="MLKKPTSAYVHIPFCTQICYYCDFSKVFIKNQPVDAYLQALIRE
                     FRSYDITELRTLYIGGGTPTSISAVQLDYLLTELSRDLNLNTLEEFTIEANPGDLTVD
                     KIEVLQKSAVNRVSLGVQTFNDKHLKRIGRSHNEVQIYSTIDALKTAGFQNISIDLIY
                     ALPGQTMDDVRSNVAKALSLNIPHLSLYSLILEHHTVFMNKMRRGKLHLPTEDLEAEM
                     FEYIISEMERNGFEHYEISNFTKPGFESRHNLMYWDNVEYYGVGAGASGYLDGIRYRN
                     RGPIQHYLKGVSEGNARLSEEVLSKNEMMEEELFLGLRKKKGVSIGKFEQKFGTSFEK
                     RYGQIVQELQSDGLLKENNGFIQMTKKGLFLGDTVAEKFIVE"
     gene            7610..8347
                     /locus_tag="ACUBV3_001519"
     CDS             7610..8347
                     /locus_tag="ACUBV3_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HHU3848014.1"
                     /translation="MGLLYRETYEVPFYESDTNHYMKLPQLLALALQISAKQSLKLGI
                     GDDIVFKRYGLVWVVTDYIIDIERLPKHAEKIVIETEAKAHNKLLCYRYFYIYGEDGQ
                     KIITISSAFVLMDFKTRKIHPVLDDITSIYQSQRIKKVIRGPKYHPIGDSKVKQYHVR
                     YFDLDMNGHVNNSKYLEWMYDVLDLDFLSSHIPKKIDLKYIKEIQYGTDIKSHWYQDG
                     LVTRHDIIGGDAIHAQARIEWQEKKED"
     gene            8348..9118
                     /locus_tag="ACUBV3_001520"
     CDS             8348..9118
                     /locus_tag="ACUBV3_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HHU3848015.1"
                     /translation="MAYKGYLIDLDGTIYKGKSRIPAGERFIERLQEKGIPYMLVTNN
                     TTRTPESVQEMLRGFNVETPLETIYTATMATVDYMNDMNRGKTAYVIGEEGLKKAIAD
                     AGYVEDTKNPAYVVVGLDWNVTYDKLATATLAIQNGALFIGTNPDLNIPTERGLLPGA
                     GSLNALLEAATRIKPVFIGKPNAIIMNKALEILNIPRNQAVMVGDNYLTDIMAGINND
                     IDTLLVTTGFTTVEEVPDLPIQPSYVLASLDEWTFNEG"
     gene            9108..>9232
                     /locus_tag="ACUBV3_001521"
     CDS             9108..>9232
                     /locus_tag="ACUBV3_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HHU3848016.1"
                     /translation="MKDKLLVVLTWIWIISLATLATIYIAWLIYPIEIQFLKLEKV"
BASE COUNT         3048 a         1432 c         1828 g         2924 t
ORIGIN      
        1 tcaaaatctg cttttgattt aacgtctccg tattcctcag gaatctctaa agataaaatt
       61 tcttcataac tagggaggac aatctcttta ccatatttgt tacggagggc agttgccaca
      121 agacggtaag ctagattagc attacgtgaa aggattggac cgtgaaagta tgacccaaaa
      181 acattcttat aatgaacccc ttcagtacca tcttctttgt tattcccatt tccataaatg
      241 acagttccta aaggtttttc gtcttctgat aaaaaggtac gtccttgatg attctcaaag
      301 ccataatatg tttcattgaa ttcatcgttg tgaatcttga tatcaccaat atagcgatta
      361 ttattttggt ttagcgtata gtgtcccata acgccaatgc cctctattcg ttctccatta
      421 gcttgaatat agtattgtcc taaaagttgg aaacctccac aaatagcaag tactacacca
      481 ttattttgaa tgaatttgtt aatgtcttct ttcttactgg gaagatcgcg tgcaacaata
      541 gcttgttcgt agtcttgtcc accaccaaaa aatgccatct gataatagtt gggatcaaat
      601 ctatcttcta atgaaacgat atcaaatgtc atttgacatc ctagtttttc tcccacatat
      661 ttcatcatta agatattccc attatcgcca taagtattta ataggttacc ataaagatga
      721 gctacattaa gagtgtattt ataatctttg gtagtaggag attttagtga ggtataagtc
      781 attaattcat ctcctttctg atagcgttgt ggttagcaag aatttcacga aactccagca
      841 tagctgtata tgttgctaaa atataagcat gctcagtctc ttgctgctca atcattgcca
      901 taatatcttg cagtttatca gcttgtttta tgcgtttctc atcataacca gttacacgta
      961 agcgacgagc aatttcagag tggcgaacac caccagcaaa tatttctggg atattcatcg
     1021 tgagaatagt ttcaaaatta gcgtcccaaa tccaagaagt atcaataccg tcagcataat
     1081 tggcatttaa tagtactgaa agagagaacg ggtatggagc aagtttaatc atatcaagag
     1141 cttgactagc tcctactgga tttttaatca gaactaaggt acatttttta tttccaattg
     1201 taaaggtttc ttgacgacca aaaacggcac ggcttttatc aaatccatct ttaatttgac
     1261 ttgcttctac accaaaatat tcagcaacag atactgccgc caaagcattg taaatattat
     1321 aaaggccacc cacgttgata ttatattgtt ggccatcaat aacgaaacct gatgaagtat
     1381 ttgtcagatg tattagcctt gttaaggcat aatcaaggtt tggacgttca aaatcacaat
     1441 taggacaagt gtagtcacca aggttggcat atgtgttcaa tcgataagtt aaaattgctt
     1501 gacactttgg acagagaata ccctcagtat tatagtgtgc taatcgtggc tcatgtttgt
     1561 cggtatcaaa tccatagaat tgcacagggt tagtcacgct tttagaatta aaaagtggac
     1621 tgtcaccgtt agctaagata gtagcttgcg gagcgttagc agctccatct aaaatcattt
     1681 gataagttgt gtagatttcg ccataacggt ccatttgatc acggaaaata ttggtaaaga
     1741 cgaacaaact aggctttata tactgtgtaa tacggggtaa actcgcttcg tctatttcaa
     1801 ggacagctat ttttttacca gatttacttt tcttagctgt taggaaggtt gaaacaatac
     1861 ctgtaatcat atttgctcca cttgggttgg ttaccacctg accgaaggct tcctttaaaa
     1921 taccaactgt taaagctgtt gttaaggttt taccgttagt accagtcaca actacaattt
     1981 cataatcttt ggcaattgta tctagtatat ctttatcgaa tttgagtgct aaactacccg
     2041 gataagtaga accacgtccc attttactta aaaggtaatg agcagactta ccggcagcca
     2101 ctcctagtgc tgtatttatt ttcataaaga tattttatca taaaaagatg ctttcttgct
     2161 aaagaaaata ttatgaaaac atggtataat gtagacgatg gaaaataaga aatcaaggag
     2221 ttaagcaaga aactcttcga caggctacca gtagagagga agatatggat attttttcag
     2281 ctattgattc aaagttttgg gcgagcatta tggaaaatcc atggatgatc cttattcatc
     2341 tcatggatat tatcatagta gctgttctga tttataaatt tatcaaagcg ctagcaggca
     2401 caaaaattat gtccttaatt cagggcgtta ttttgtttat catcattaga tttgtgtcag
     2461 aatggatagg gctaacaacg attacatttt tgatgaatca gattgttact tatggtgtaa
     2521 ttgcaggtgt tgtcatcttt gctccagaga ttagaacagg tctcgaaaaa tttgggcgaa
     2581 caccacaact atttacgcaa cgttctcaat taagcagtga tgaaaagcta gtagatgccc
     2641 ttgttaaggc agttgcttat atgagtccgc gtaaaatagg agccttgatt tcaattgaac
     2701 gtacccaaac tttgcaagag tatatagcta caggtatccc attagatgca gatatttcca
     2761 gtgaattgtt aattaatatt ttcattccta atacaccact gcacgatggt gctgttattg
     2821 taaaagataa aaaaattgct acagcatgtt cgtacttacc tctatcagaa tcatcttcga
     2881 tttcaaaaga atttggaaca cgccacagag cagcgattgg tctatcagag aattcagatg
     2941 cactaacggt tattgtttct gaagagactg gtggtatttc tgttgctttg aaaggtgaat
     3001 ttttacatga tctttctaaa gatagctttg aagcaattct gagaactcag ttgattcaaa
     3061 atcaagagga aaactcaaaa ttagcttggt ataatcaact attgaggagg aagtagacat
     3121 gaaaaaattt tttacaaata aattttggtt aggcgttgtc tcactctttc tggctatttt
     3181 attattttta acggcaactg caacaagtat gaaccatcaa gacaatagta agattgcagg
     3241 agcgtctgag acatatacac ataccctaac agatgtgcca atagacatca aatacgatag
     3301 tgatgattac tttattagtg gctattctta tggggcagat gtctatatgt catcggttaa
     3361 tcgtgttaag ctagattctg aaataaatga ggatacacga aaatttaaag tagtcgctga
     3421 tttgacaaat atgaaaccag ggactcacaa agtcccacta aaggtagtaa atttgccatc
     3481 tggtgtaaat gctacggttt ctccaacaac aattacagta actatgggta aaaagaaaac
     3541 aaaagagttt ccggtttatg gacatgttaa tgataagcaa attaaagctg gttatgcagt
     3601 cgataagatg tcagttgatg tgtcaaaagt taaagttact agtgatgaat ctattataga
     3661 tcgtattgac catgtcgctg ccaatatccc tgatgataaa gttcttgatg atgattttaa
     3721 caagacagtt actttacaag ctgtaacagc agatggtaca gtattagcaa gtattattca
     3781 cccttctaaa gcaactttat ctgtcaaggt aaaaaaattg accaagacag taccgattaa
     3841 tttgatacca gtaggacaat tcagcgatag tatttcaaaa attaactaca agttgagtca
     3901 agaaaaagca gtaatttcag gaacaaaaga ggctttagaa gctatttctg ttattaatgc
     3961 tgaggtagat atttcagacg taactaaaaa tacagaaaag aaaattaatc tttctgcaaa
     4021 caatgtttca gttgatcctg ctcaagtaac agttcaattg acaactacta aaaaataata
     4081 gataataaaa acgaaaaaaa tggaggtagc gtttttcgct agaaaaaaat tatgggaaaa
     4141 tattttggta cagatggtgt tcgcggtgaa gctaatgttg aactgacgcc agaattggct
     4201 tttaaattag gacgttttgg aggatatgtt ctaagtcaac acgaaactga tcgtccgaga
     4261 gtttttgtag ctcgtgatac tcgtatttct ggtgaaatgc tggaatctgc tttgattgct
     4321 ggtctacttt cagtaggtat tgaggtttat aaacttggtg ttttagccac tcctggagtg
     4381 tcttaccttg ttcgtacaga aaaagctagt gcaggggtta tgatttcagc aagccacaat
     4441 ccagcacttg ataatggtat taagttcttt ggtagtgatg gttttaaact tgatgatgat
     4501 cgtgaattag aaattgaagc tttacttgat gcgaaggaag acactttacc acgtccatca
     4561 gcacaaggtt taggtacttt agtagattat cctgaaggac ttcggaaata tgagaagttt
     4621 atggaatcaa ctggtattga tttagaaggt atgaatgttg cgttggatac agcaaatggt
     4681 gcagcaacag cctcagctcg gaatatcttt ttagacttga atgctgatat aagtgttatt
     4741 ggtgatcaac cagatggttt gaatatcaat gatggtgttg gttcgacaca tcctgagcaa
     4801 ttacaaagtt tagttagaga aaatggttcc gacatcggtc tagcttttga tggtgatagt
     4861 gatcgtttga tcgctgttga tgaaaatgga gagattgtag atggtgataa aattatgttt
     4921 atcattggga aatatctctc tgataaaggg cagcttgctc agaatacaat cgtaacaacg
     4981 gttatgtcaa atctcggctt tcataaagcg ctcgaccgtg aaggaattca taaagcgatt
     5041 accgctgtag gagaccgcta cgtggttgaa gaaatgcgta aatcaggata taatcttggc
     5101 ggagagcaat caggccacgt tattatcatg gattacaata ctacagggga tggacaatta
     5161 acagccatcc aattgactaa ggtcatgaaa gaaactggta agaaattatc agagttagct
     5221 agtgaagtga cgatttatcc tcaaaaatta gtaaatattc gtgttgaaaa taacatgaaa
     5281 gataaagcga tggaagttcc tgcaattgcg gaaattattg ctaaaatgga agaagagatg
     5341 gatggcaatg gtcgtattct agtacgtcct agtggtactg agcctcttct tagagttatg
     5401 gcagaagcgc caacaaatga agcggttgat tattatgttg atactatcgc agatgtcgtt
     5461 cgtacagaaa ttggtttaga ttaaaacggc gttattctgc tataaagtcg tcaatgttat
     5521 aaaataataa agagagggaa ttggagtgga gcatcaagca tacgcctttc tctctctttt
     5581 tgatttgaga agggagttta gagaaaatga ctaaagaagc agaattatta tgggctaaac
     5641 acaagtattt agtcttaagt aaatctcaga aaatttattt ggatattaga caaactttga
     5701 aatcaccaaa ttgtaccgtt ttagacgttc agtcactcat tgaccaagca gtgcttttag
     5761 aagaatcacc atcacaagtg actaatgctt atatgcatat atggggctat tttaaaaata
     5821 aggcagagcg tcaagaaaaa gaagaatttt taaccttgct ggaaaaatac aggaaaacag
     5881 gttatcaacg aagaaaactg ttagcttttc taaaacagct attagctaaa tacccaaatt
     5941 cttatttaca aaactcttct attttcgaag aagagtaaaa gattgatagt tgaggtatat
     6001 aaatgagatt atggcatcaa gatattattg agttattgcc taggcagcaa cttttaggac
     6061 agcatagaga gtgttgtgca ttgagaggga atgggtgggg gagaaaacat gagactgttg
     6121 attatgtttt tcgatattca ccttataggt tatttgcata tcatcaatta gttatggaag
     6181 aaatgatgga gcgtgggtat cgtgtatcta aagaatggct aattgcagaa tataggggaa
     6241 tgaagtgccc aagatatgat acacttaatc ctgttgactt ggagacacct atctatcctg
     6301 agcacaatca agactatcta caagaatgtc tatggaattt aaaagcaaaa ggaattgacc
     6361 ttcctataaa taagataaaa tagagattta tctctatttt tattttggaa taaaaagcta
     6421 tattatcaag ttttagagga gtgttttttt aagggcaaag tggtaaaata gaaacatgtt
     6481 aaagaaacca acctcagcat atgtacacat tccattttgt acccaaatat gttattattg
     6541 tgatttttca aaagttttta ttaaaaatca gcctgtagac gcttatcttc aggctcttat
     6601 ccgtgaattt cggtcttatg atattacaga attgcgcacc ttatatattg gaggagggac
     6661 acctacatct ataagcgctg tgcaactgga ctatttattg acggagttaa gtagagattt
     6721 aaacttaaat actttagagg agttcactat cgaagctaat ccaggagatt taaccgttga
     6781 taaaatagag gtgcttcaaa agtcagctgt caatagagtt tctctaggag ttcagacctt
     6841 taatgataaa catttaaagc gtattggacg cagtcacaat gaggtacaaa tttattctac
     6901 aattgatgct cttaaaacag ctggttttca aaatatctct attgacctaa tttatgcctt
     6961 acctggacaa acgatggatg atgttagaag taatgtagcc aaagctctct ctctaaatat
     7021 tcctcatttg agtctatata gtttaatttt agaacaccat actgtattta tgaataaaat
     7081 gcgtcgtggg aaattacatt tgccaacaga agatttagaa gccgagatgt ttgaatatat
     7141 catttctgaa atggagagaa atggttttga gcattatgaa atttctaatt ttacaaagcc
     7201 gggttttgaa agtcgtcata atttgatgta ctgggataat gtagaatatt atggtgttgg
     7261 tgcaggtgct tctggttatc ttgatggtat tcgttatcgt aatagagggc caatacaaca
     7321 ttatctaaag ggtgtttcag aaggtaatgc aaggttatct gaagaagtat tatcaaaaaa
     7381 tgaaatgatg gaagaggagc ttttcctcgg tcttcgtaaa aaaaagggtg tttccatcgg
     7441 aaaatttgaa caaaaatttg gaacgtcctt tgagaaacgt tacggtcaga ttgttcaaga
     7501 actccaaagt gatgggcttt tgaaagaaaa taatggtttt atccagatga ctaaaaaagg
     7561 tctcttctta ggagatacgg ttgccgagaa atttattgtg gagtaaaaaa tgggtttatt
     7621 atatcgagaa acttacgaag tgccttttta tgaatcagac actaatcatt atatgaaatt
     7681 accgcagttg ttggctttag cgttacaaat ttcagccaaa caatctttaa aattaggaat
     7741 aggcgatgac atcgttttta aaagatacgg attggtttgg gtcgttacag attatattat
     7801 tgatattgag cgtttaccca agcatgctga aaagatagtc attgagactg aagccaaagc
     7861 tcataataaa ttattgtgtt accgctattt ttatatttat ggtgaagacg ggcaaaaaat
     7921 aataacgatt tcatctgctt ttgtcttaat ggattttaaa acacggaaga ttcatcctgt
     7981 tcttgatgat attacttcaa tttaccaatc ccaaagaatc aaaaaagtta ttcgagggcc
     8041 gaaatatcat cccattggag atagtaaagt taaacaatac catgttcgtt attttgattt
     8101 agatatgaat ggacatgtta ataatagtaa gtatctagaa tggatgtatg atgtgttgga
     8161 cttagatttt ctaagtagcc acattcctaa aaaaattgat ttaaaatata ttaaggaaat
     8221 tcaatatgga acggatatta agtctcattg gtatcaagat ggtcttgtga cacgtcatga
     8281 tattattggt ggcgatgcta ttcatgctca agctaggatt gaatggcagg aaaaaaagga
     8341 ggattaaatg gcttataaag gatatcttat tgatttagat ggtactattt acaaagggaa
     8401 aagtcgtatc ccagcaggag agcgttttat cgaacgtttg caagaaaaag gcattcctta
     8461 tatgttagta actaataata cgactcgcac accagaaagc gttcaggaaa tgcttcgagg
     8521 cttcaatgtt gaaacaccac ttgaaacaat ctataccgca actatggcta cggttgacta
     8581 tatgaatgat atgaaccgtg gaaaaacagc ttacgttatt ggtgaagaag ggcttaaaaa
     8641 agctattgca gatgcaggtt atgtggagga tacaaaaaat ccagcctatg tggttgttgg
     8701 tttagattgg aatgttactt atgataaatt ggcaactgct acgttagcaa ttcaaaatgg
     8761 tgctttattt attggaacca atccagattt gaatattcct actgaacgtg gtttattacc
     8821 cggtgctggc tccttaaatg ctttattaga agccgcaacg cgtataaaac cagtctttat
     8881 tggaaagcca aatgccatca ttatgaataa ggcacttgaa attttaaata ttcctagaaa
     8941 tcaagcagtc atggtcggtg ataattattt gacagatatc atggctggta ttaataatga
     9001 tattgatact ttgttagtaa caacgggttt tacaacagtt gaagaagtac cagatttacc
     9061 aattcaacca agttatgttt tagcaagttt agatgaatgg acatttaatg aaggataagt
     9121 tattagtagt tttaacttgg atttggatta tatcgttagc aactctagct acaatttata
     9181 ttgcttggtt gatctatcct atagaaattc agtttttaaa attagaaaag gt
//
LOCUS       DBJYSH010000083         9110 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.083, whole genome shotgun sequence.
ACCESSION   DBJYSH010000083 DBJYSH010000000
VERSION     DBJYSH010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 9110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9110
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.083"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            31..1221
                     /locus_tag="ACUBV3_001522"
     CDS             31..1221
                     /locus_tag="ACUBV3_001522"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000514054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HHU3848017.1"
                     /translation="MGKEKLILAYSGGLDTSVAIAWLKKDYDVIAVCMDVGEGKDLDF
                     IHDKALTIGAIESYILDVKDEFAEHFVLPALQAHAMYEQKYPLVSALSRPIIAQKLVE
                     MAHQTGATTIAHGCTGKGNDQVRFEVAIAVLDPELKVIAPVREWKWHREEEITFAKAN
                     GVPIPADLDNPYSIDQNLWGRANECGVLENPWNQAPEEAFGITKSPEEAPDCAEYIDI
                     TFQNGKPIAINNQEMTLADLILSLNEIAGKHGIGRIDHVENRLVGIKSREIYECPAAM
                     VLLAAHKEIEDLTLVREVSHFKPILENELSNLIYNALWFSPATKAIIAYVKETQKVVN
                     GTTKVKLYKGSAKVVARHSSNSLYDENLATYTAADNFDQDAAVGFIKLWGLPTQVNAQ
                     VNKG"
     gene            1240..2628
                     /gene="argH"
                     /locus_tag="ACUBV3_001523"
     CDS             1240..2628
                     /gene="argH"
                     /locus_tag="ACUBV3_001523"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - L-arginine biosynthetic process
                     via ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HHU3848018.1"
                     /translation="MTKNHKLWGGRFESSLEKWVEEFGASISFDQKLAPYDMKASMAH
                     VTMLGKTDIISQEEAGLIKDGLKILQDKYRAGQLTFSISNEDIHMNIESLLTAEIGEV
                     AGKLHTARSRNDQVATDMHLYLKDKLQEMLKKLLHLRTTLVNLAENHIYTVMPGYTHL
                     QHAQPISFGHHLMAYYNMFTRDTERLEFNMKHTDLSPLGAAALAGTTFPIDRHMTTRL
                     LDFEKPYSNSLDAVSDRDFIIEFLSNASILMMHLSRFCEEIINWCSYEYQFITLSDTF
                     STGSSIMPQKKNPDMAELIRGKTGRVYGNLFSLLTVMKSLPLAYNKDLQEDKEGMFDS
                     VETVSIAIEIMANMLETMTVNEHIMMTSTETDFSNATELADYLASKGIPFRKAHEIVG
                     KLVLECSKNGSYLQDIPLKYYQEISELIENDIYEILTAKTAVKRRNSLGGTGFDQVKE
                     QILLARKELKAE"
     gene            2769..3650
                     /locus_tag="ACUBV3_001524"
     CDS             2769..3650
                     /locus_tag="ACUBV3_001524"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HHU3848019.1"
                     /translation="MAIVSAEKFVQAARDNGYAVGGFNTNNLEWTQAILRAAEAKKAP
                     VLIQTSMGAAKYMGGYKLCKQLIETLVESMGITVPVAIHLDHGHYDDALECIEVGYTS
                     IMFDGSHLPVEENLEKAREVVAKAHAKGISVEAEVGTIGGEEDGIVGKGELAPIEDAK
                     AMVETGIDFLAAGIGNIHGPYPANWEGLDLDHLKKLTEAVPGFPIVLHGGSGIPDDQI
                     QEAIKLGVAKVNVNTECQLAFCQATRDYAVEFNANEAEYMKKKLFDPRKFLKPGFDAI
                     TEAVEERIDVFGSANKA"
     gene            complement(3735..4652)
                     /locus_tag="ACUBV3_001525"
     CDS             complement(3735..4652)
                     /locus_tag="ACUBV3_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000197051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HHU3848020.1"
                     /translation="MTRKIGIIGMGHVGSTVAHALIAQGIGDDYVFIDTNIDKVNADM
                     IDFQDALANATYHANIVLNDYDALSDADVVISALGDISLQNNKEANRFAELPFTSKEV
                     YDVSQKLKSSGFRGILIVVTNPVDAITQLYQEYTQFPKSRVIGTGTLLDTARMKRVLG
                     QRLQVDPRSIGGYNLGEQGNSQFVAWSQVTVKGQPAISLLKAEELEQIKEASLKGGHT
                     VFFGKKYTSYGIATAAVRIVLAVLNDSHELLPVSTFYESEGVYLGYPAIIGRVGIVER
                     ISLSLSPKEEQQLTDSAAFIRNYLKHINF"
     gene            4891..5079
                     /gene="rpmB"
                     /locus_tag="ACUBV3_001526"
     CDS             4891..5079
                     /gene="rpmB"
                     /locus_tag="ACUBV3_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HHU3848021.1"
                     /translation="MAKVCYFTGRKTVSGNNRSHAMNQTKRTVKPNLQKVTVLIDGKP
                     KKVWVSARALKSGKVERV"
     gene            5211..5576
                     /locus_tag="ACUBV3_001527"
     CDS             5211..5576
                     /locus_tag="ACUBV3_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018365170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HHU3848022.1"
                     /translation="MTVKINTRDGQIELSDDVIATVVGGSATEIFGVVGMASKSAIKD
                     NFQALLRKENYAKGVVVRSSEAGIAVDVYTVMSYGVKISEVSKNIQERVKFNLENQLG
                     LSADMVNVYVQSIKVVGED"
     gene            5576..7240
                     /locus_tag="ACUBV3_001528"
     CDS             5576..7240
                     /locus_tag="ACUBV3_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HHU3848023.1"
                     /translation="MSKITTSLFQEMVQAASTRLSKQAEYVNSLNVFPVPDGDTGTNM
                     GMTMENGAKEVSDKPATTVGEVGQILSKGVLMGARGNSGVITSQLFRGFGQSIKDKEE
                     LTGQDLAHAFQNGVEVAYKAVMKPVEGTILTVSRGAATAALKKAEETDDAVEVMRATL
                     KGAKRALAKTPEMLPVLKEVGVVDSGGQGLVFIYEGFLSALTGEYIASEDFKATPATM
                     TEMVNAEHHKAVVGHVATEDIKYGYCTEVMVGLKQGPTYVKEFNYEEFQGYLSNLGDS
                     LLVVNDDEIVKVHVHTEDPGLVMQEGLKYGSLVKVKVENMRNQHDAQMQKVEVEETVK
                     ETKEYGIIAVVAGDGLAEIFKSQGVDYIISGGQTMNPSTEDIVKAIEKVNARNVIILP
                     NNKNIFMAAQSAADVVDIPAAVVETRTVPQGFTSLLAFDPAKSLETNVADMTNSLSDV
                     ISGSVTLAVRDTTIDGLEIHENDILGMVDGKILVSTPDMEKALKDTFDKMIDEDSEIV
                     TIYVGEDGKQALAETLSEYLEETYEDVEVEIHQGDQPVYPYLMSVE"
     gene            7388..8272
                     /locus_tag="ACUBV3_001529"
     CDS             7388..8272
                     /locus_tag="ACUBV3_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HHU3848024.1"
                     /translation="MSLIILTVILVLVIVLLITSLYVVKQQTVAIIERFGKYQKTATS
                     GIHIRVPLGIDKIAARVQLRLLQSEIIVETKTKDNVFVTLNIATQYRVNENNVTDAYY
                     KLIKPEAQIKSYIEDALRSSVPKLTLDELFEKKDEIALEVQHQVAEEMSTYGYIIVKT
                     LITKVEPDAEVKQSMNEINAAQRKRVAAQELANADKIKIVTAAEAEAEKDRLHGVGIA
                     QQRKAIVDGLADSIQELKDANVTLTEEQIMSILLTNQYLDTLNTFAINGNQTIFLPNN
                     PEGVEDIRTQVLSALKTR"
     gene            complement(8364..8480)
                     /locus_tag="ACUBV3_001530"
     CDS             complement(8364..8480)
                     /locus_tag="ACUBV3_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HHU3848025.1"
                     /translation="MAEKTDLSSAYRRLKSPNIKTRKRALKIIHEYKRYGKK"
BASE COUNT         3085 a         1447 c         1837 g         2741 t
ORIGIN      
        1 aaaacatagg aatttggaag gggatttttt atgggaaaag aaaaattaat tttagcttat
       61 tctggtggtt tagatacctc tgttgctata gcttggttaa aaaaagatta tgacgttatt
      121 gctgtctgta tggatgttgg cgaaggtaaa gatcttgatt ttattcacga taaggcttta
      181 acaataggtg ccatagaatc ttatatttta gatgttaaag atgagttcgc tgagcatttt
      241 gtgttaccag ctttacaggc acatgcgatg tatgaacaaa aatacccgct tgtttcagcg
      301 cttagtcgtc caattattgc tcaaaaatta gttgaaatgg cacatcaaac aggtgcgacc
      361 acaattgccc atggatgtac aggtaaagga aatgaccaag tgcgttttga agttgcaatt
      421 gcagtactag atcctgaatt aaaagtaatt gcccctgttc gtgagtggaa gtggcatcgt
      481 gaagaggaaa ttacttttgc aaaagctaac ggcgtaccta ttccagcaga tttagataac
      541 ccttattcta tagaccaaaa tttatgggga cgtgccaatg aatgtggtgt gcttgagaat
      601 ccttggaatc aagcgccaga agaagctttt ggtattacaa aatcaccaga agaagctcct
      661 gattgtgcag aatatattga tattacgttc caaaatggga agcctatagc tataaacaat
      721 caagaaatga cactagcaga tttgatttta tcgctaaatg aaattgcagg aaaacatggt
      781 attggacgca ttgatcatgt tgaaaatcgt ttagtcggta tcaaatcacg tgaaatttat
      841 gaatgtcctg cggcaatggt actgttagct gctcataaag aaattgaaga tttaacatta
      901 gtccgtgaag tgtctcattt taaacctatc cttgaaaatg aactatcaaa tcttatttat
      961 aatgctttat ggtttagtcc agctacaaaa gctatcattg cctatgtcaa agaaacacaa
     1021 aaagtggtta atggtactac aaaagttaag ttatataaag gttctgccaa agtcgttgct
     1081 cgccactcgt ctaattcatt atatgatgag aacttagcaa cttatacagc tgctgataac
     1141 tttgatcaag atgcagcagt tggttttatc aaactctggg gacttccaac ccaagttaat
     1201 gcacaagtaa acaaaggata aataaaggaa gataagagga tgacaaaaaa tcataagtta
     1261 tggggtggtc gttttgaaag tagtcttgaa aaatgggttg aagagttcgg tgcatcaatc
     1321 agtttcgacc aaaagttagc tccctatgat atgaaggctt ctatggccca tgtcacaatg
     1381 ttaggcaaaa cggatattat tagtcaagaa gaagcaggct tgataaaaga tggtttaaaa
     1441 atactgcaag ataaatacag agcaggtcaa ttgactttta gcatatcaaa tgaagacatt
     1501 catatgaaca tagagagctt attaaccgct gaaattggag aagtagcagg taagcttcat
     1561 actgcacgtt cacgaaatga tcaagtggct acagatatgc acctttattt gaaagataaa
     1621 ttgcaagaaa tgttgaagaa attgcttcat ttacgaacaa ccttagttaa tttagcagaa
     1681 aatcatatct atactgttat gccaggatat acacatttac aacatgctca gccgatttca
     1741 tttggacatc atctaatggc ttactataat atgtttacgc gtgatacaga gcgattagag
     1801 tttaatatga aacacaccga tctatcacca ttaggagcag cagctctagc agggaccacg
     1861 tttcctattg accgccatat gacaactcgt ttattagatt ttgaaaaacc atatagtaat
     1921 tcgcttgatg ctgtctcaga tcgtgatttt atcatagaat ttttatccaa tgctagcatt
     1981 ttaatgatgc atttatcacg tttttgtgaa gaaatcatta attggtgcag ttatgaatat
     2041 caattcatca cattatcaga tactttctct acaggatcat ctattatgcc tcaaaagaaa
     2101 aatcctgata tggcagaatt aattcgaggg aagacaggcc gtgtttatgg aaatttattt
     2161 agtttactaa ctgttatgaa atcattgccg ttagcttata acaaagattt acaagaagat
     2221 aaagaaggga tgtttgatag cgtagaaaca gtcagcatag ctattgaaat catggctaat
     2281 atgcttgaga caatgactgt gaatgagcac ataatgatga cctcaacgga gactgatttt
     2341 tcaaatgcaa cagaattagc agattattta gcaagtaagg ggataccttt cagaaaagca
     2401 catgaaattg taggtaaatt ggttttagaa tgcagtaaaa atggttctta tttacaggat
     2461 atacctttaa aatattatca agaaatttca gagttgatag aaaacgatat ctatgagata
     2521 ttaactgcaa agacagcagt taaacgaaga aattcattag gaggaacagg gtttgaccag
     2581 gttaaagaac agatcctgtt ggcacgtaaa gaattgaaag cggaatgact gctttctgaa
     2641 aaaaacctcg tttaactctg ttaagtttct cacgtatttt cagtaaaaaa ctagcatttt
     2701 ttatagaatt gtgctagaat taacatgtaa gtgggcaacg cccaaaaaat aaataggagg
     2761 cctgaataat ggcaatcgtt tcagcagaaa aattcgtcca agcagctcgt gataacggct
     2821 atgcagtagg tggatttaac acaaacaacc ttgagtggac tcaagctatc ttgcgcgcag
     2881 cagaagctaa aaaagctcca gtacttatcc aaacttcaat gggtgcggct aaatacatgg
     2941 gtggttacaa actttgtaaa caacttattg aaactttggt tgaatcaatg ggtattactg
     3001 taccagttgc tatccacctt gaccacggtc attacgatga tgcccttgag tgtattgaag
     3061 taggttacac ttcaattatg tttgatggtt cacaccttcc agttgaagaa aaccttgaaa
     3121 aagcacgcga agttgttgct aaagcacatg ctaaaggtat ttcagtagaa gctgaagttg
     3181 gtactatcgg tggtgaagaa gatggtattg tcggtaaagg tgaattagca ccaatcgaag
     3241 acgctaaagc tatggttgaa actggtattg acttcttggc tgcaggtatc ggtaacattc
     3301 atggaccata cccagcaaac tgggaaggac ttgaccttga tcacttgaag aaattgactg
     3361 aagctgttcc tggtttccca atcgtgttac acggtggttc aggtattcct gatgatcaaa
     3421 tccaagaagc tatcaaactt ggtgttgcta aagttaacgt taacacagaa tgtcaattag
     3481 ctttctgtca agcaactcgt gactacgctg ttgaattcaa cgctaacgaa gctgaataca
     3541 tgaagaaaaa actctttgac ccacgtaaat tcttgaaacc tggtttcgat gcaattacag
     3601 aagcagttga agaacgtatt gacgtgtttg gttcagcaaa caaagcataa ttaacataaa
     3661 acagataata gttaaaagct cacttatggt gagctttttt tggattatat gactttttat
     3721 taaatagata ctctttaaaa attgatatgc ttaagataat ttcgaataaa tgccgctgaa
     3781 tctgtgagtt gttgctcttc ttttgggctt agtgaaagcg aaattctttc tacaattcct
     3841 actctaccaa taatagctgg atatccaagg taaacccctt cggactcata gaaggtagat
     3901 acaggtagta actcatggga atcatttaac acagctaaaa cgatgcggac agctgcggta
     3961 gcgataccat aactagtata ttttttacca aaaaagacag tgtgcccgcc ttttaaagaa
     4021 gcttctttta tctgttctaa ttcttctgct tttaatagtg atatagctgg ttgtcctttt
     4081 acagttacct gactccaagc gacaaattgg gagttaccct gctctcctag attataacca
     4141 ccgatactcc ttggatcaac ttgtaggcgt tgaccaagca ctcttttcat ccgagcagta
     4201 tccaataagg ttcctgttcc gataacgcgt gatttaggaa attgagtata ttcttgataa
     4261 agctgggtga tggcatcaac aggattagta acgacaataa ggattcctct aaaaccagat
     4321 gattttaact tttggctaac gtcgtagact tctttactgg taaatggtag ttcagcaaag
     4381 cgattagctt ctttgttatt ctgtagtgaa atatcaccta aggctgaaat tacaacatca
     4441 gcatctgaca acgcgtcata gtcgtttaat actatattag cgtgataagt agcatttgcc
     4501 agagcatctt gaaaatcaat catatcagca ttaaccttat caatattagt atcaataaag
     4561 acataatcat caccaatacc ttgtgcaata agagcgtgtg caacagttga tccaacgtgc
     4621 cccatgccta taattccaat tttacgtgtc atatgaaacc tcctttgatt attgttgtct
     4681 attataatat tttgaaagaa aatgtaaact aaaaaatgaa tcagaaggaa gtgtgtctta
     4741 ttataaaaaa tatgtaagcg atttagataa aacaaatgaa aaagtaattt tgaaaagaac
     4801 ttgctagaag aagtaagttg tgatagaata gtctagtatg tggtgctagc acatccgtaa
     4861 atattgatca atataggagg aactcgtaaa atggctaaag tatgttattt cacaggacgt
     4921 aaaacagttt ctggtaacaa ccgttcacac gcgatgaacc aaacaaaacg tactgttaaa
     4981 ccaaaccttc aaaaagttac tgttcttatc gacggtaaac caaaaaaagt ttgggtttca
     5041 gctcgtgcgc ttaaatctgg taaagtagaa cgcgtttaat aaactgagaa agccttaggg
     5101 ctttttttag tgtttctaag gctgatttga tagttagatg cctctatttt tttacaataa
     5161 tagtgagata tgatagaata aagtgataaa aactttttga ggtagtagat atgactgtaa
     5221 aaatcaatac aagagatggt caaattgaac tctctgatga cgttattgca actgtcgttg
     5281 gaggttcagc aacggaaatt tttggcgttg ttggtatggc aagtaaaagt gctatcaaag
     5341 ataactttca agcacttttg cgtaaggaaa actacgcaaa aggtgttgtt gtgaggtcta
     5401 gtgaagcggg cattgcagta gatgtttata ctgtcatgag ttacggcgtc aaaattagtg
     5461 aagtttcaaa aaatattcaa gagcgagtga aattcaatct cgaaaatcag cttggtttat
     5521 ctgctgatat ggtgaatgtt tatgtgcaaa gtattaaggt tgtaggagaa gattagtgtc
     5581 aaagattacg acaagtttat tccaagaaat ggtacaagct gccagcacac gtctgagtaa
     5641 acaggcagag tatgtcaact cattaaatgt attcccagtt ccagatggtg atactggtac
     5701 gaatatgggg atgactatgg aaaacggagc caaggaagtt tcggacaaac cagcgacgac
     5761 tgttggagaa gttggtcaaa ttctttctaa aggtgtattg atgggagcac gtggaaattc
     5821 aggtgtaatt acatcacaat tattccgtgg ttttggtcaa agtatcaagg ataaagaaga
     5881 attaacgggt caagatttgg cacacgcttt ccagaatggt gttgaagtag cttataaagc
     5941 cgttatgaaa ccagttgaag gaactatctt gacagtatca cgcggtgcag ctaccgctgc
     6001 tctaaagaaa gctgaagaaa ctgatgatgc agttgaagtt atgcgcgcaa ctctcaaagg
     6061 tgctaagcgt gctcttgcta aaacaccgga gatgctacct gttcttaaag aggttggtgt
     6121 tgttgattct ggtggtcaag gactcgtctt tatttacgaa ggtttcctct cagcactgac
     6181 tggggaatat atcgcatcag aagattttaa agcaactccc gcaacaatga ctgaaatggt
     6241 taatgcagag caccataaag ctgtagtagg ccatgtagcc acagaagata ttaaatatgg
     6301 ctattgtaca gaagtcatgg ttggtttaaa gcaaggacca acttacgtta aggaatttaa
     6361 ctacgaagaa tttcaaggtt atcttagcaa tctaggggat tctttactag ttgttaatga
     6421 tgatgaaatc gtcaaggttc atgttcatac agaagatcca ggtcttgtta tgcaagaagg
     6481 tttgaaatat gggtcgcttg tgaaggttaa agttgaaaac atgcgtaacc aacatgatgc
     6541 ccaaatgcaa aaggttgaag ttgaagaaac tgtcaaagaa actaaagaat atggtattat
     6601 tgctgttgta gctggtgacg gtctagctga gatttttaaa tcacaaggtg ttgattatat
     6661 catctctggc ggccagacta tgaacccatc tacagaagat attgtaaaag cgattgaaaa
     6721 agtaaacgct agaaatgtta ttattttacc aaacaataag aacatcttca tggctgcaca
     6781 gtcagctgca gatgtcgttg atattccagc agcagtagta gagacacgta cagttccaca
     6841 aggatttact agcttacttg cttttgatcc tgctaagtct cttgaaacta atgtagctga
     6901 tatgacaaat agcctctcag atgtcattag tggtagtgtg actttagctg ttcgtgatac
     6961 aacgatagat ggccttgaaa ttcatgaaaa cgacatcctt gggatggttg atggtaaaat
     7021 tctagtatct acaccagata tggaaaaagc acttaaagac acttttgata aaatgattga
     7081 tgaagatagt gaaatcgtga caatctatgt tggtgaagat ggtaaacaag cattagccga
     7141 gaccttgtcg gaatatcttg aggagactta tgaagatgtt gaagttgaga tccaccaagg
     7201 agatcaacct gtatatccat acctcatgag cgttgaataa ttgataaaat aaaaaattag
     7261 gagagatact tctaattttt tattttatca gagtttctat tttagtatga taaagcatca
     7321 gtattttgaa aattttgaca aattgtgtta gaattgtttt aaaacattca tacaaaggag
     7381 atttatgatg tcattaatta ttcttacggt tattttggtt ttagtgattg ttctgttgat
     7441 aacttcttta tacgttgtta aacaacagac agtagccatc attgaacgct ttggtaaata
     7501 tcaaaagact gctactagtg gtattcatat tcgtgtacca ttgggtatag ataagattgc
     7561 agcacgagtg cagttacgtc ttttacagag tgaaattatc gttgagacaa aaacaaaaga
     7621 taacgttttt gtcactttaa atattgctac acaatatcgt gtcaatgaaa ataacgttac
     7681 agatgcttat tacaaattaa taaagccaga agctcagatt aaatcttata tcgaagatgc
     7741 tttgcgttca tcagtaccta aacttacatt agatgagcta ttcgaaaaga aagatgaaat
     7801 agccttggaa gttcaacatc aagtggcaga agaaatgtca acatacggct acatcattgt
     7861 taagacttta attactaagg ttgagccaga tgcagaagta aaacaatcca tgaacgaaat
     7921 caatgctgca caacgtaaac gtgtagcagc tcaagaatta gcgaatgctg ataagattaa
     7981 aattgtaact gctgcagaag cagaagcaga aaaagatcgt ctacatggtg tgggtattgc
     8041 gcaacaacgt aaagcaattg tagatgggtt agctgattcg attcaagaat tgaaagatgc
     8101 taacgtgaca ttgacagaag aacaaatcat gtctatcttg ttaacaaatc aatatttaga
     8161 tactttaaat acatttgcta ttaatggtaa tcaaacgatt ttcttaccaa ataacccaga
     8221 aggtgttgaa gatattcgta cacaagtttt atctgctctt aaaacaagat aatgaacttt
     8281 ttatagaagc aaaataacca gagcatttgc tctggttatt ttgtgtctga cttatttatt
     8341 ataattggag agacggaaaa aagttatttt ttaccgtaac gtttgtactc atgaatgatt
     8401 ttcagagcac gttttctagt tttaatgtta ggacttttaa gtctgcgata ggctgacgat
     8461 aggtctgttt tttcagccat gattacctcc tatagacaat ttattaacgt taacagacac
     8521 cagtatacca caaaaaaatt aactggtaaa gtactttgtt gataaattta aaaataaata
     8581 gcaaagtaat taaatatcaa tcaaaagtaa taagctcaaa gatatattaa acctttatta
     8641 atgaggtttg gatttgacaa gcgatgttaa gtgaaagatg aatgaggaaa atacccaacg
     8701 aaaaagtagc taatggctat catgtgcaca taaaaaaacg atcgttgagt gatcgttttt
     8761 cagcaaacta aataattatt tttaatgtaa ttgaatgaaa atttatactt gtgaaagtgt
     8821 atcggttaag ttaataaggt cagtttttat ttatcgtaag cacttaggaa gtgttttgga
     8881 gcgtacccac gagagtagat aaacccggca tcgcgtgaag gataagacac tgtgtctgcc
     8941 ttcgcaagac ttgctgaagt agctaataat agagtgacag ctgaagctgc tgttaaaaca
     9001 ctgattttaa gtttcattat aaaatccttt caaagataat aaactattct tgcacagtgg
     9061 ttagttcatt ttgtgcatac catattatat catcaaaagc tcattcgaac
//
LOCUS       DBJYSH010000084         8826 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.084, whole genome shotgun sequence.
ACCESSION   DBJYSH010000084 DBJYSH010000000
VERSION     DBJYSH010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 8826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8826
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.084"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            36..1319
                     /gene="aroA"
                     /locus_tag="ACUBV3_001531"
     CDS             36..1319
                     /gene="aroA"
                     /locus_tag="ACUBV3_001531"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HHU3848026.1"
                     /translation="MKLLTNANTLKGTIRVPGDKSISHRAIIFGSISQGVTRIVDVLR
                     GEDVLSTIEAFKQMGVLIEDDGEIITIYGKGFVGLTQPNNLLDMGNSGTSMRLIAGVL
                     AGQEFEVTMVGDNSLSKRPMDRIALPLSKMGARISGVTNRDLPPLKLQGTKKLKPIFY
                     HLPVASAQVKSALIFAALQTKGESLIVEKEQTRNHTEDMIRQFGGHLDIKDKEIRLNG
                     GQSLVGQDIRVPGDISSAAFWIVAGLIIPNSHIILENVGINETRTGILDVVSKMGGKI
                     KLSSVDNQVKSATLTVDYSHLQATHISGAMIPRLIDELPIIALLATQAQGTTVIADAQ
                     ELKVKETDRIQVVVESLKQMGADITATADGMIIRGNTPLHAASLDCHGDHRIGMMIAI
                     AALLVKEGEVDLSGEEAINTSYPNFLEHLEGLVNA"
     gene            1312..1824
                     /locus_tag="ACUBV3_001532"
     CDS             1312..1824
                     /locus_tag="ACUBV3_001532"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001127191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HHU3848027.1"
                     /translation="MPKVLLGFMGVGKTSVANCLENEVIDMDSLIEKHIGMSISRFFT
                     EEGEASFRALESQFLNELLKKKNEGLVIASGGGIVLLEENRRLLTLNRHNNILLTGSF
                     EILYHRIKKDEKNRRPLFLNHSKEEFYDIYQKRMLLYSGLSDMIIDTDYLTPQKIATV
                     IGESLVNLSN"
     gene            1881..3254
                     /locus_tag="ACUBV3_001533"
     CDS             1881..3254
                     /locus_tag="ACUBV3_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000089330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HHU3848028.1"
                     /translation="MSRNNYGQLNHHEELRYNYLLKNIHYLNEREKMEFQYLHYKKTA
                     VRPQRRTESPPTNSYYEEPYSDSYYQDDDFYSEPQLTSQGLPIYQEERAPKKKKQRAR
                     KEKQRVKVMAPFPPKAITPPRKKKKFKGFLKFIGIILLIVLSGMVFMFVKGMRDVNNG
                     KSHYSPAIIEDFKGKDAVDGTNILILGSDKRISERSTDARTDTIMVANVGNKDNKVKM
                     VSFMRDLLVNIPNYSTEGYYDMKLNASFNLGEQDNHKGAEYVRQTLKNHFDIDIKYYV
                     MVDFETFADAIDTLFPNGVKINAKFGLVGGQSADSVKVPDDLRMKNGVVPSQKIKVGI
                     QYMDGRTLLNYARFRKDDDGDFGRTQRQQQVMRAIVSQIKDPRRLFTGSAAIGKAYAL
                     TSSNLSYSFVLTDGIPILSDAKNGIKQMTIPREGDWVDDYDQYGGQGLTIDFAKYKKI
                     LKKMGLR"
     gene            3355..4716
                     /gene="rlmD"
                     /locus_tag="ACUBV3_001534"
     CDS             3355..4716
                     /gene="rlmD"
                     /locus_tag="ACUBV3_001534"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HHU3848029.1"
                     /translation="MLHKNDIIETEISDISHEGMGIAKVDGFVFFVENALPGEIIQMR
                     VLKLRKRIGYGKVEEYLTTSPHRNEGLDYTYLRTGIADLGHLTYEQQLLFKQKQVADN
                     LYKIAHISDVLVEPTLGMTIPLAYRNKAQVPVRRVDGQLETGFFRKNSHTLVSIEDYL
                     IQEKEIDALINFTRDLLRKFDVKPYDEEQQSGLIRNLVVRRGHYTGQLMLVLVTTRPK
                     IFRIDQMIEKLVSAFPSVVSIMQNINDRNSNVIFGKEFRTLYGSDTIEDQMLGNTYAI
                     SAQSFYQVNTEMAEKLYQKAIDFSDLNSEDIVIDAYSGIGTIGLSVAKQVKHVYGVEV
                     VEKAVSDAKENATRNGITNSTYVADSAENAMAKWLKEGIKPTVIMVDPPRKGLTESFV
                     YSAAQTKADKITYISCNSATMARDIKLFEELGYHLVKIQPVDLFPMTHHVECVALLQR
                     SKG"
     gene            4954..5326
                     /locus_tag="ACUBV3_001535"
                     /pseudo
     CDS             4954..5326
                     /locus_tag="ACUBV3_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656693.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tip lysozyme"
     gene            5814..5990
                     /locus_tag="ACUBV3_001536"
     CDS             5814..5990
                     /locus_tag="ACUBV3_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000557948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3848030.1"
                     /translation="MHVGKSYFDYQENALIPQLGFLYSKLTKKIELDNRLSPTEQKLL
                     ITLLLHTKGLIIDM"
     gene            complement(7071..7385)
                     /locus_tag="ACUBV3_001537"
                     /pseudo
     CDS             complement(7071..7385)
                     /locus_tag="ACUBV3_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011680908.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            7335..7604
                     /locus_tag="ACUBV3_001538"
     CDS             7335..7604
                     /locus_tag="ACUBV3_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3848031.1"
                     /translation="MVSLLLLKVKQEELIEFHNVFYREAISSYLETENFEKAKVTEEN
                     YQVFLGIRTYQKSYNYQDHSENLKGNIEVAMIGVENNISDEHIFC"
     gene            7665..8816
                     /locus_tag="ACUBV3_001539"
     CDS             7665..8816
                     /locus_tag="ACUBV3_001539"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HHU3848032.1"
                     /translation="MVRKFLKSLLSLFLIAVIATGISVACFFFIPENKGNITPILLHR
                     FMRKNNVNGMMIVSDNTGKPITISHGINRGEVETDIENNKLFPMASLQKLMTGIIIQR
                     LIDQDVLSEDDRLSQFFPQVKGSNSITIHQLLTHTSGLREKGVKVSPYLKNEREQLQF
                     CLKHYNFVNKKSWYYSNINFSFLTGIATQVTGRTYAELVDDVIKNPLRLDDIQSYQSV
                     VNHDLVSPMRKNGKLNKINIFNQVSTAYGAGDFFTTPLNFWVLMRSFSKGYFFPTDEY
                     TKHQNDAISHYYGGLYMHGRIVNSNGAFFQYSSFGYADLKTHDTMVLFMNNKVYSDAS
                     HVAPKAFEYYQKFMFLNKIFHLVFYFVFSFFLMLLIRHSFRKRRYKKRH"
BASE COUNT         3004 a         1286 c         1717 g         2819 t
ORIGIN      
        1 atacaataat agcaaaatag attttgggag ttatcatgaa attactgact aatgcaaata
       61 ctctcaaggg aaccatccgt gttcctggcg ataaatcaat aagtcacagg gctattattt
      121 ttgggagtat atctcaggga gttacccgaa ttgttgatgt cttaagaggc gaggatgttt
      181 tatcaactat agaggctttt aaacaaatgg gggttctaat tgaagacgat ggcgagataa
      241 tcactatcta tggcaaagga tttgtaggtt taacacaacc gaataatctc ttagacatgg
      301 gaaactctgg tacttcaatg cgtctgatag caggtgtgtt agctggtcaa gagtttgaag
      361 tgacgatggt tggagacaat agtttgtcaa agagacctat ggatcgtata gcattgcctt
      421 taagcaaaat gggagctaga atttcgggag taactaaccg tgatttacca ccattaaaac
      481 ttcaaggtac aaaaaaatta aaacctattt tttaccattt acccgtagct tctgcacaag
      541 taaaatcagc tttaattttt gcggctttac aaactaaggg agaatctctc attgtagaaa
      601 aagagcaaac aaggaatcat actgaggaca tgattcggca atttggtgga catttggata
      661 ttaaagataa agaaattcga ctcaatggcg gtcagagctt agtaggacaa gatataagag
      721 ttcctggtga tatttcgagt gctgcctttt ggatagtagc gggtcttatt attcctaaca
      781 gtcatattat tttagaaaac gtaggtataa acgagacacg aactggaatc cttgatgtcg
      841 tcagtaaaat gggtggaaaa attaagttat cttcggttga taatcaagta aaatcagcaa
      901 cccttacagt tgattactct catctacaag ctactcatat ttctggggct atgattccac
      961 gtctgattga tgaattacct attattgctc ttttagcaac acaagctcaa ggcaccacag
     1021 tcatagcaga tgctcaggaa ttaaaggtga aagagacaga tcggattcag gtcgttgtcg
     1081 aatcgctaaa acagatgggt gctgatatta ctgctacagc agatggtatg attatacgtg
     1141 gtaatacccc tcttcacgct gcttcacttg attgtcatgg agatcataga ataggaatga
     1201 tgatagctat tgctgctctc ttggtcaaag aaggcgaagt agatttatct ggtgaagaag
     1261 ctattaatac aagctatcct aactttttag aacatttgga gggattagtt aatgcctaag
     1321 gtattattgg gattcatggg agttggcaag actagtgttg ccaactgctt agaaaatgaa
     1381 gtaattgata tggatagtct gattgaaaaa catattggta tgtcaatttc ccgatttttt
     1441 acagaagaag gagaagcctc ctttcgtgct ttagaatctc aatttttaaa cgaactttta
     1501 aagaaaaaaa atgaaggact tgtgatagct agtggaggag gtattgtact ccttgaagaa
     1561 aatcgtcgtc ttttaacttt aaatcgtcat aataatattc ttttaacagg aagttttgaa
     1621 atcttatatc atcgtataaa aaaagatgag aagaatagaa gaccattatt tttaaaccat
     1681 tcaaaagaag aattttatga tatttatcaa aaacgcatgt tactctatag tggtttgtct
     1741 gatatgatta ttgataccga ttatcttaca ccacaaaaaa tagctactgt aattggagag
     1801 tcactggtta atttatctaa ctaagttatc acagtttggt agtatgaact attattattg
     1861 aaagtaaatc acctgaaaat atgtcaagaa ataattacgg tcagctaaat catcatgaag
     1921 agctacgata taactatctt ttaaaaaaca tccattatct taatgaacgt gaaaagatgg
     1981 aatttcagta ccttcattat aaaaaaacag cagtacgacc acagagaagg acagaaagtc
     2041 caccaactaa ttcttattat gaggaacctt attctgattc ttattatcaa gatgacgatt
     2101 tttactcaga gcctcagcta acctcacaag gtttaccaat ttatcaagaa gagagagctc
     2161 cgaagaagaa aaaacaaagg gccagaaaag aaaaacagag ggtgaaggtt atggcgcctt
     2221 tccctccaaa ggctataacc ccccctagaa agaaaaaaaa atttaagggt ttcctaaagt
     2281 tcataggtat tatattacta attgttttat caggtatggt tttcatgttt gttaaaggaa
     2341 tgcgtgatgt gaataatggg aagtcacatt atagccctgc aattattgaa gattttaagg
     2401 gaaaagatgc tgttgatggg actaatatct taattttagg tagtgataaa cgtatcagcg
     2461 agagatctac tgatgcgaga acagatacca taatggttgc taatgttggt aacaaggata
     2521 ataaggttaa gatggtgagt tttatgcgag atctcttagt caatattcct aattatagta
     2581 cggaaggtta ctatgatatg aaattaaatg cgtcttttaa tttgggagaa caagataatc
     2641 ataaaggagc agagtatgtt cgacaaacgt tgaaaaatca ttttgatatt gatataaaat
     2701 attatgttat ggttgacttt gagacatttg cagatgctat tgatacactg tttcctaatg
     2761 gtgtcaagat taatgctaaa tttggacttg tcggtggaca atctgctgat tcagttaaag
     2821 ttcctgatga tttaaggatg aaaaatggcg ttgtacctag ccaaaaaatt aaggttggga
     2881 ttcagtatat ggatggaagg acccttctaa attatgctcg tttcagaaaa gacgatgatg
     2941 gggactttgg tcgaacacaa cgtcaacaac aagttatgag agctattgtc tctcaaataa
     3001 aagacccacg tcgtctcttt acaggttctg ctgctatcgg taaagcctat gctttaactt
     3061 caagtaattt atcttatagc tttgtcctaa cagatggcat tccaatttta tcagatgcta
     3121 aaaatggtat taagcaaatg acaattccaa gagaaggaga ttgggtagat gattatgacc
     3181 agtatggtgg tcaagggtta acaattgatt ttgctaagta caaaaagatc ctaaagaaaa
     3241 tgggactgcg ttaacatttt taattatgat ataatagagt gatttattgt cactctattt
     3301 ttatctttag tgtataaact aaagacttca aatgatagat ataaaaggaa aattatgtta
     3361 cataaaaatg atattattga aactgaaatt agcgacatta gccatgaggg aatgggtatt
     3421 gccaaagtag atggctttgt tttctttgtt gaaaatgctc ttccaggtga gattattcag
     3481 atgcgcgtct tgaaattacg taagcgtata ggatatggaa aagttgagga atatttaacg
     3541 acttctcctc accgaaatga aggattagac tatacctatt tacgtacagg gattgctgat
     3601 ttgggacatc ttacttatga acaacagctg ttatttaaac aaaaacaagt agctgataac
     3661 ctttataaaa ttgctcatat atctgatgtt ttagtagaac ctactcttgg tatgacaatc
     3721 cctttggcat atcgaaataa agctcaggta ccagttcgtc gtgttgatgg acagctagag
     3781 acaggttttt tcagaaaaaa ttcacatact ctggtatcca ttgaagatta tttaattcag
     3841 gaaaaagaga ttgatgctct gattaatttt acacgagatt tattaagaaa atttgatgtt
     3901 aaaccatatg acgaagagca gcaatctggt ttaattagaa atctcgttgt tcgtcgagga
     3961 cattatacag ggcaattaat gttggttttg gtgacaacac gtcctaaaat attccgcatt
     4021 gaccaaatga ttgaaaagtt ggtgtctgcc tttccttctg ttgtttcaat tatgcaaaat
     4081 attaatgatc gtaatagcaa cgttattttt gggaaagaat ttcgtactct atatggttct
     4141 gatactattg aggaccaaat gttaggaaat acttacgcta tatcagccca atctttttac
     4201 caagttaata ctgaaatggc tgaaaaactg taccaaaaag caatcgattt ttcagattta
     4261 aactcagagg atatcgttat tgatgcctat tcaggtatag gaacaattgg cttatctgtt
     4321 gctaaacaag taaaacatgt ttatggtgtt gaagttgttg aaaaagcagt aagtgatgcc
     4381 aaagaaaatg ctactcgaaa tgggataact aacagtacct atgttgctga ttcggctgaa
     4441 aatgcaatgg caaagtggct taaagaaggt ataaaaccaa ctgttataat ggttgatcca
     4501 ccacgaaaag gattaacgga gagttttgta tattctgctg cccaaacaaa agcagacaaa
     4561 atcacttata tttcttgtaa ttcagcgaca atggcgcgtg atatcaaact atttgaagaa
     4621 ttagggtatc atttagtaaa aattcaacca gtagacttat tcccaatgac acatcatgtg
     4681 gagtgcgtag cgttgctaca acgaagcaaa gggtaaaaat cctttatttt aagcactttt
     4741 tcaagcattt tgtctttatt gaaaagagtg attttaacat aaaaaaggta ttaaaaaaca
     4801 tattgacgtg accgtttgtt ttgaagtggc ttgcgtagac aaaaaaatag atacgtcaga
     4861 taaatttctg gcattacgag aacattttta gagtgttctc tttttttagt ttacggagga
     4921 aaaatatata tggaaaaaca ggattcacga gttctcatcc attgggaggg aaattctggg
     4981 gacaagctta ttgaacacca aaccagcgca acggggtggt actaccaagt cgatcgtagc
     5041 tttagtcaac caaaagggga accgcccaga atgatccaaa ggcactagaa agtgtccgta
     5101 atgattcgat ttcgggcggt gatgatgtca tgggttatgc ttatagcaaa tgtacttggg
     5161 gagttgcggc acgaattaat cagtgggact gaaactcaaa ggttgaaatg gtgagaagat
     5221 taccattacc agttcaatgg gaaatggtca ggattgggtt ggaacagccg aaagactaga
     5281 tggtgaaact gatacagttc caaaagaagg tactattctc tctttttagg aaagtagtta
     5341 tggttcgtat ataggctacg gaactatatc tttcgtcaca ttacatctac agatagtacc
     5401 atgaattttg cttatatgac caagtaaagt gaggatatac taacaaatga aatatttatt
     5461 atcgtatttg tccattttat cgaaaagttt gcatattatc attatgtttg ataagatgca
     5521 aatataatga tagtaggagc taaatatgga tatttaaaaa atcaagagta tcctaagtgc
     5581 tttccatttt gaaattcaaa tatagctaat agttctagaa cttctaattg tttttcgtcg
     5641 acgatatgaa ttttcaatct taactgttag gattccacct ccctttggtt aaagaaaaaa
     5701 ggtcaggtcg tttagataac tttgtcaaac aagctcaagc tatctaaaaa tagtttgaaa
     5761 tgggcattac tctagttttt aataagctat ctgatgagca gaaggagaag ttaatgcatg
     5821 ttgggaagtc ttattttgac tatcaagaaa atgctcttat cccacaatta ggttttctat
     5881 attctaaatt aactaaaaaa attgaacttg ataatcggtt gtctccgact gaacaaaagt
     5941 tattgattac cttattatta catactaaag gtttaatcat tgatatgtaa gaagtaagtc
     6001 agctaaccga tctttctatt ctaaaactta tattgttgct ttagaaattt taaagagcgt
     6061 ggatggcttc ataataaaca gaaatcttac caatttgcga agccaaaaaa tatgatattt
     6121 gaagagtcca aagatctaat agatagtcca gttagagaag cgttgattat aagtgataag
     6181 gattttcaaa aattaaaaca agagctatta ttttaaccga cttattttaa agacttatca
     6241 tatctaggct tgcttgatga ttcggaaaaa tacggagact atactatttc aaggaaaaga
     6301 tacaaaagtt tcgaatcaag tcttcaacta tacatccttc aaagtcatcg gctagagatt
     6361 tggaattatg aaccaatccc tttgattact agaaaaataa atagcttgga gaggctaacg
     6421 tgacactggt tgatccaatc tcgctttatt taacactaag aatgatgaag accctcgtat
     6481 tgaagaagaa gttgagcagc tagaagataa gattttgaga atataatcgt tttttcgtgc
     6541 tacactaact tgtcagggtt gcaacagaca aaaaaataat ttaggaggga ttcgttatgc
     6601 gtacaaagta tataggatat gaaattgttt taaggggtgg ttattaatat ggataagaaa
     6661 aaggttgttt caactatcgt atctacaggg tttcttatag tttcaatatc aaattttttt
     6721 agagagctat cacctatact ttttctcttg aatattattg gatttttctg cttcctatta
     6781 ttattttctg ccaacataaa taaattgttc ttaaaacgat agccgttata taggatatgg
     6841 tatgtatgca tttggtgaaa ttataggtaa aacgaatgga atagtactta ctcctcaaag
     6901 ctatcgtgag agatataaaa ggatatcgag gagtcactag atagttggat ctcattgaaa
     6961 aaatttgttt aaagtatctc atataagcat aaaacgagga atcaatcaaa atttcttgat
     7021 tgattccttg ttttttggct ctttgtcaac tgtagtgggt tgatgaaaaa ttatattctg
     7081 gagaaaacca aattggtttt ctcctttttg atattcaaag tgatatgaat tctcttttta
     7141 aaatattcaa agtttctaaa gctaaatgtt tgtgcttgat gattttgatg agattattgg
     7201 ttgctcaagt ttggtgtttg aataaggcga ttcaaggaca ttgataacct tatctctgtc
     7261 tttaaaaaat gtcctaaaaa cagtttaaaa gattggagtg acactagaga aagtgtttta
     7321 aacagggtaa agaaatggtg agcttgcttc tcttgaaagt aaagcaggag gagttgatag
     7381 agtttcataa tgttttttat agagaagcga tatcttctta tcttgaaaca gaaaattttg
     7441 agaaggctaa agtgactgaa gagaattacc aagttttttt aggaatccgt acttatcaga
     7501 agagttataa ctatcaagat catagtgaga atctaaaagg aaatattgag gtagcaatga
     7561 taggagttga aaataatatt agcgacgaac atattttttg ctagaatata taaaatgtct
     7621 ttgaaataat ttagccctgt gttatgatat taaggaggtt atttatggta cgtaaatttt
     7681 taaaatcgct cctatctctc tttcttatag cagtaatcgc caccggtatt tcagtggcat
     7741 gtttcttttt tattccagaa aataaaggga atattacacc gattttattg catcgcttta
     7801 tgcgaaaaaa taatgttaat ggaatgatga ttgttagcga taatactggt aaaccgatta
     7861 ccatctcgca tgggattaac cgtggggaag ttgaaacgga tattgaaaat aataaattat
     7921 ttccaatggc ttctctccaa aaactcatga caggaattat tatccaaaga ttaattgatc
     7981 aagatgtctt gagtgaagac gaccgattgt ctcagttctt tccccaagta aaaggtagta
     8041 atagtattac catccaccaa ttgttgaccc atacgagtgg gttaagagaa aaaggtgtta
     8101 aggtgtctcc ttatctaaaa aatgagagag aacaattaca attttgctta aaacactata
     8161 actttgttaa taagaaatcg tggtattatt ctaatatcaa ctttagtttc ctaacaggta
     8221 tagccactca agttacggga aggacctacg ctgaacttgt tgatgatgtg ataaaaaatc
     8281 ctttgcgatt agatgatatc caaagttacc aatcagttgt taatcatgac ttagttagcc
     8341 caatgcgaaa gaacggcaag ttaaataaaa ttaatatctt taatcaagta tccacagcat
     8401 atggagctgg agattttttc acaactccgc tgaacttttg ggtgttgatg cggtcttttt
     8461 ctaaagggta ctttttccca acggatgaat ataccaaaca tcaaaatgat gcgatatcac
     8521 attattatgg tgggctttac atgcatggtc gtatagttaa ttctaatggg gcctttttcc
     8581 agtattcaag ttttggttat gcagatctaa agacgcatga tacaatggtg ctttttatga
     8641 ataacaaagt ctatagtgat gctagtcacg tggcaccaaa agcatttgaa tattatcaaa
     8701 aatttatgtt ccttaataag attttccact tagtctttta ctttgtattt tctttctttt
     8761 taatgctttt aatacgtcat tcgtttagga aaagaaggta taagaaacgt cactaaatca
     8821 catgaa
//
LOCUS       DBJYSH010000085         8743 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.085, whole genome shotgun sequence.
ACCESSION   DBJYSH010000085 DBJYSH010000000
VERSION     DBJYSH010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 8743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8743
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.085"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            complement(61..1086)
                     /locus_tag="ACUBV3_001540"
     CDS             complement(61..1086)
                     /locus_tag="ACUBV3_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004793 - threonine aldolase activity
                     [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine aldolase family protein"
                     /protein_id="HHU3848033.1"
                     /translation="MLHFENDYNKGAHPELLNALIETNDQGLSGYGTDSYCQQAADKI
                     REVCSCPQAEVEFLVGGTQTNQVVISSMLASYEGVIAAETGHVSSHEAGAIEFSGHKV
                     LTLPSHNGKLLASEVATYIETFYADGNYQHMVFPGMVYISHPTEYGTLYSKAELEELS
                     KICKHYQIPLFIDGARLGYGLAAKDTDVDFPTIAALSDVFYIGGTKMGALAGEAVVFT
                     KKNRPKQFTTIVKQHGALLAKGRLLGLAFDRFFTDDLYLKIGKHAIDLAEELKIILEE
                     KGYSFYLKSPTNQQFVIVENTKLADLAKNVAYSFWEKYDDHHTVIRLATSWSTSREDV
                     TALRNVL"
     gene            complement(1206..2216)
                     /gene="tsaD"
                     /locus_tag="ACUBV3_001541"
     CDS             complement(1206..2216)
                     /gene="tsaD"
                     /locus_tag="ACUBV3_001541"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HHU3848034.1"
                     /translation="MKDRYILAVESSCDETSVAILKNDKELLANIIASQVESHKRFGG
                     VVPEVASRHHVEVVTTCFEDALQEAGIVASDLDAVAVTYGPGLVGALLVGMAAAKAFA
                     WANKLPLIPVNHMAGHLMAARDVKELQYPLLALLVSGGHTELVYVSGPGDYKIVGETR
                     DDAVGEAYDKVGRVMGLTYPAGREIDQLAHKGQDTYHFPRAMIKEDHLEFSFSGLKSA
                     FINLHHNAEQKGEALVLEDLCASFQAAVLDILLAKTQKALLKYPVKTLVVAGGVAANQ
                     GLRERLATDISPDIDVVIPPLRLCGDNAGMIALAAAIEFEKENFASLKLNAKPSLAFE
                     GL"
     gene            complement(2292..2699)
                     /gene="rimI"
                     /locus_tag="ACUBV3_001542"
     CDS             complement(2292..2699)
                     /gene="rimI"
                     /locus_tag="ACUBV3_001542"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 - protein-N-terminal-alanine
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HHU3848035.1"
                     /translation="MESSEQALAIWSVLSDVYDKSPWSLSQISEDLKKDSTDYFFVYN
                     DGEVIGFLALQQLVGEVEITNIAVKKNYQGKGYAYQLMSMIADIEVPVFLEVRYSNIV
                     AQKLYERCGFVVLGKRKNYYHDPIEGAIVMRKG"
     gene            complement(2701..3393)
                     /gene="tsaB"
                     /locus_tag="ACUBV3_001543"
     CDS             complement(2701..3393)
                     /gene="tsaB"
                     /locus_tag="ACUBV3_001543"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HHU3848036.1"
                     /translation="MMKVLAFDTSSKALSVAVLNNMECLATVTINIKKNHSINLMPAI
                     DFLMQSIDLEPQDLDRIVVAEGPGSYTGLRVAVATAKMLAYTLKIDLVGVSSLYALTN
                     GFSENDLLVPLIDARRNNVYVGFYQNGDTVKPDCHTSLEEVLQEVGNKANVHFVGEVA
                     AFFDQIKKALPHAKITETLPCAVAIGRKGQKMESVNVDAFVPRYLKRVEAEENWLKNH
                     CETNTEEYIKRV"
     gene            3572..3802
                     /locus_tag="ACUBV3_001544"
     CDS             3572..3802
                     /locus_tag="ACUBV3_001544"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase subunit epsilon"
                     /protein_id="HHU3848037.1"
                     /translation="MIYKVFYQETKERNPRREQTKTLYVTIDAANELEGRIAARKLVE
                     ENTAYNIEFIELLSDKHLEYEKETGVFELTEF"
     gene            3856..5535
                     /gene="rnjA"
                     /locus_tag="ACUBV3_001545"
     CDS             3856..5535
                     /gene="rnjA"
                     /locus_tag="ACUBV3_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J1"
                     /protein_id="HHU3848038.1"
                     /translation="MSNINLKPEEVGVYAIGGLGEIGKNTYGIEYQDEIIIVDAGIKF
                     PEDDLLGIDYVIPDYSYIVENIDRIKALVITHGHEDHIGGIPFLLKQANLPIYAGPLA
                     LALIKGKLEEHGLLRDATLYEIHANTELTFKNLSVTFFRTTHSIPEPLGIVIHTPQGK
                     VICTGDFKFDFTPVGEPADLHRMAALGEDGVLCLLSDSTNAEVPTFTNSEKIVGQSIM
                     KIIEGIDGRIIFASFASNIFRLQQAAEAAVKTGRKIAVFGRSMEKAIVNGIELGYIKV
                     PKGTFIEPSELKNLHASEVLIMCTGSQGESMAALARIANGTHRQVTLQPGDTVIFSSS
                     PIPGNTTSVNKLINTIQEAGVDVIHGKINNIHTSGHGGQQEQKLMLRLIKPKYFMPVH
                     GEYRMQKVHAGLAVDTGIPKENIFIMENGDVLALTSDSARIAGHFNAQDIYVDGNGIG
                     DIGAAVLRDRHDLSEDGVVLAVATVDFDSKMILAGPDILSRGFIYMRESGDLIRESQH
                     ILFNAIRIALKNKDASIQSVNGAIVNALRPFLYEKTEREPIIIPMVLTPDK"
     gene            complement(5697..6206)
                     /locus_tag="ACUBV3_001546"
     CDS             complement(5697..6206)
                     /locus_tag="ACUBV3_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HHU3848039.1"
                     /translation="MEENMNIKQLKSKTMLGTVALVSAFSFASTNADANTYNYAVDVD
                     YLASAEEIAQAHPASNTFPLGQCTWGVKEMATWAGNWWGNGGDWAASATSAGYTVGTQ
                     PRVGSIVCWNDGSYGHVAYVTAVDPVTNKIQVLESNYAGHQWIDNYRGWFDPQNTATP
                     GVVSYIYPN"
     gene            complement(6354..7700)
                     /gene="glnA"
                     /locus_tag="ACUBV3_001547"
     CDS             complement(6354..7700)
                     /gene="glnA"
                     /locus_tag="ACUBV3_001547"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012997923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HHU3848040.1"
                     /translation="MTITAEDIRREVKEKNVTFLRLMFTDILGVMKNVEIPATDEQLD
                     KVLSNKAMFDGSSIEGFVRINESDMYLYPDLDTWIVFPWGDENGAVAGLICDIYTAEG
                     EPFAGDPRGNLKRNMKRMQEMGYKSFNLGPEPEFFLFKMDENGNPTLDVNDKGGYFDL
                     APTDLADNTRREIVNVLTQMGFEVEASHHEVAVGQHEIDFKYDDVLKACDNIQLFKLV
                     VKTIARKHGLYATFMAKPKFGINGSGMHCNMSLFDNEGNNAFFDPEDPRGMQLSEDAY
                     YFLGGLMEHAYNYTAIINPTVNSYKRLVPGYEAPVYIAWAGRNRSPLIRVPASRGMGT
                     RLELRSVDPTANPYLALSVLLGSGLEGIENKIEAPEPIETNIYAMTVEERRQAGIVDL
                     PSTLHNALKALEEDEVVKAALGTHIYTNFLDAKRIEWASYATYVSQWEIDNYLDLY"
     gene            complement(7734..8105)
                     /locus_tag="ACUBV3_001548"
     CDS             complement(7734..8105)
                     /locus_tag="ACUBV3_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HHU3848041.1"
                     /translation="MKERELRRTMAVFPIGTVMKLTDLTARQIRYYEDQGLITPERTE
                     GNRRMFSLNDMDRLLEIKDFISDGLHISDIKNEYMQRQHKSKEKQKSLSDAEVRRLLQ
                     DELRNQGRFSSPSQHIGNMHL"
     gene            complement(8185..8724)
                     /locus_tag="ACUBV3_001549"
     CDS             complement(8185..8724)
                     /locus_tag="ACUBV3_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000854122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HHU3848042.1"
                     /translation="MKTLKKLLSNYKFDIKKFKLGMRTFKTGLSVFLVLLVFHLFGWK
                     GLQIGALTAVFSLREDFDKSVHFGFSRIIGNSIGGLLSLVFFAFNEIFHQAFWVTLLI
                     VPICTMLCIMINVACNNKSGIIGGTAALLIITLSIPSGETILYVFARIFETFCGVFIA
                     MMVNTDIEILRKKLKNNKL"
BASE COUNT         2783 a         1797 c         1380 g         2783 t
ORIGIN      
        1 aaaatcaaga cgattatttt gtaatagtca ctactgaata atatttcttt tattaaaaac
       61 tcacaagaca tttcttaaag cagttacatc ttcgcgacta gttgaccaac tagttgctag
      121 acgtattaca gtatgatggt catcatattt ttcccaaaaa ctataagcta cattctttgc
      181 taaatctgct agttttgtat tttctacaat aacaaattgt tgattagttg gtgattttaa
      241 atagaacgag taaccttttt cttctaaaat gatttttaat tcctctgcta agtcaatcgc
      301 atgctttcca atctttaagt aaaggtcatc cgtaaagaaa cgatcaaaag caagccccag
      361 caatcttccc ttggctaata acgcaccatg ctgctttaca attgtagtaa actgttttgg
      421 acgatttttc tttgtgaaaa caactgcttc cccagcaaga gcccccattt tggttccacc
      481 aatgtaaaaa acatctgata aggctgcaat cgttgggaaa tcaacatctg tatctttcgc
      541 agccaaacca taccccagac gagcaccgtc aataaataag gggatttgat aatgtttaca
      601 aatcttcgat aactcttcta gttctgcttt actataaagt gttccatatt ctgttggatg
      661 tgatatataa accattcctg gaaaaaccat gtgttggtaa ttaccatcag cataaaaagt
      721 ctcaatataa gttgcaactt cgcttgcgag taatttccca ttatgagaag gtaaagtcaa
      781 taccttatga cctgaaaatt ctatggctcc tgcttcatga ctactcacat gacctgtttc
      841 tgcagcaata actccttcat aagaagcaag catactactg ataactactt gatttgtttg
      901 agtaccaccc actaaaaact caacttcagc ttgtggacag gaacagactt ctcgaatttt
      961 atctgccgct tgttgacagt aactatctgt cccatatccc gatagacctt gatcattagt
     1021 ttcaataagg gcattcaaaa gctctggatg agctccttta ttataatcat tttcaaaatg
     1081 taacattcct taactccttt attagttaaa aaaaatagcg acaatatcat ttgctaattt
     1141 tttgaatatt caattattat accaaataaa aaatatcaaa acaagcgttt tgatattatg
     1201 aacacctata aaccttcaaa agctaagcta ggtttggcat tcaattttaa agaagcaaaa
     1261 ttctcttttt caaactctat cgctgctgct aatgcaatca ttcctgcatt atccccacat
     1321 aatctaagag gaggaataac cacatcaata tcaggagaaa tatcagtagc caagcgttcc
     1381 cgaagtcctt gattagctgc aactccacca gcaacgacta aagttttcac tggatacttt
     1441 aacaaagctt tttgagtttt ggccaataaa atatccaaaa cagccgcctg aaaggaagca
     1501 cataaatctt caagaaccaa tgcttcaccc ttttgttctg cattatgatg taaattgata
     1561 aatgcagatt ttaatccaga aaaagaaaat tcaagatgat cttctttgat catcgctcta
     1621 ggaaaatggt aagtatcttg acccttatga gctaactgat caatctcgcg acctgctgga
     1681 taagttaagc ccataacacg gcctacttta tcataagctt ctccaacagc atcatcccga
     1741 gtttctccta ctattttgta atctcccggt ccagaaacat atactaattc tgtatgtccc
     1801 ccactgacaa gcaaagctaa caatgggtat tgaagttcct taacgtcacg tgctgccatt
     1861 aaatgacctg ccatgtggtt tacaggaatt agaggtaatt tatttgccca agcgaaagct
     1921 tttgctgcag ccatacctac caataaggct cctactaatc ccggaccata tgttacagca
     1981 acagcatcca aatcgctagc aacaatacct gcttcttgaa gagcatcctc aaaacaggtt
     2041 gttactactt caacgtgatg acggcttgcc acttcaggaa caacaccacc aaaacgtttg
     2101 tgactttcaa cttgacttgc aataatatta gctagtaact ctttatcatt ttttaaaata
     2161 gcaacactag tttcatcaca tgaactttcc actgctaaaa tatatctatc tttcataatg
     2221 atattaatcc atcaatatcg tcttttccat tagtattgag gactattcat atcctttcta
     2281 tcatcaacta tttatccctt gcgcatcact atagcacctt caattggatc atggtaataa
     2341 tttttgcgct ttcctaaaac tacaaaacca cacctctcat aaagtttttg tgctacgata
     2401 tttgaataac gaacctcaag aaaaactggc acctctatat cagcaatcat tgacattaac
     2461 tggtaagcat aacctttacc ttgataattt tttttaacag caatattagt aatttctact
     2521 tcacctacca attgttgaag agccaaaaaa ccaatgacct caccgtcatt ataaacaaaa
     2581 aaataatctg ttgaatcttt ttttaaatct tctgaaatct gcgataaact ccacggagat
     2641 ttgtcataga catcagatag tacgctccat atagccagtg cttgttctga tgattccatc
     2701 tcaaactctc ttaatatatt cttctgtatt cgtttcacag tggtttttta accaattttc
     2761 ctcagcttca actcgtttta agtatcgtgg aacaaacgca tctacattaa cgctttccat
     2821 tttttgtcct ttgcgcccaa ttgccactgc acaaggtaaa gtttctgtaa ttttagcatg
     2881 tggtaaggct ttcttaatct gatcaaaaaa tgctgcaacc tctccgacaa aatgaacatt
     2941 ggctttattc cccacctctt gtaagacttc ttcaagagaa gtgtgacagt ctggtttaac
     3001 agtatcacca ttttgataga aaccaacata aacattgtta cgtcgtgcat ctataagtgg
     3061 taccaacaaa tcattttctg aaaatccatt tgttaaagcg tacaggctag atactccaac
     3121 taagtcaatc ttaagcgtat aagctagcat ttttgctgta gcaacagcta cacgtaagcc
     3181 cgtataagat cctggaccct ctgccactac gatacggtcc aaatcttgag gttctaaatc
     3241 aattgattgc attaaaaaat caatggctgg catcaaatta atgctatgat tctttttgat
     3301 attgatagtg acagtcgcta aacattccat attgtttagt acagccactg atagtgcttt
     3361 gcttgaagta tcaaaggcta aaactttcat catttaactt cccttacttg attctacctc
     3421 ttattttatg atataatcat gataattgca acgaatcaag gttaattcta ttactctata
     3481 aaaacttaat aatggtttgt cattgtctca cttaacggca ttgacccatt tttttatagt
     3541 tagaaaaacc gagagataag aaaggattat tatgatttat aaagttttct atcaagaaac
     3601 aaaagaacgc aacccacgtc gtgagcaaac caaaacactt tacgttacta ttgatgcagc
     3661 taatgaatta gaaggtcgca ttgctgcacg taaactagtt gaagaaaata cagcttacaa
     3721 tatcgaattc atcgagttac tttcagacaa acaccttgaa tatgaaaaag aaactggtgt
     3781 tttcgagctc actgagttct agaaaggagt taaacataat agctagagta atctacccat
     3841 tatgataaac ttattatgtc aaatatcaac ttaaaacccg aagaagttgg ggtttatgcg
     3901 attggtggtc tcggtgaaat cggaaaaaat acttacggta tcgaatatca agacgaaatt
     3961 attattgtag acgctggtat taaattccct gaagatgatc ttttgggaat agattatgtt
     4021 atcccagact attcatatat cgttgaaaac attgaccgta tcaaggccct tgttataact
     4081 catgggcacg aggaccacat cggtggtatt ccttttctat taaaacaagc taatttaccg
     4141 atttatgcag gtcctctcgc tcttgctcta atcaaaggta agttagaaga acatggttta
     4201 cttcgtgacg ctacattata cgaaatccac gctaatacag agctgacttt taaaaactta
     4261 agcgttacat ttttccgcac tactcactcc attcctgaac cattaggtat tgttattcat
     4321 acccctcaag gtaaggttat ttgtacaggt gacttcaaat ttgactttac acctgttggg
     4381 gagcccgctg acttacatcg tatggctgct ctaggtgaag atggtgtcct ctgcctttta
     4441 tcagactcga caaatgctga agttccaacc ttcactaatt ccgaaaaaat tgttgggcaa
     4501 tctattatga agattatcga aggtatcgac ggacgtatta ttttcgcctc ttttgcttca
     4561 aacatcttcc gactccaaca ggctgctgaa gctgctgtta aaactggtcg taaaattgct
     4621 gtttttggac ggtcaatgga aaaggcaatt gttaacggta ttgaacttgg ctatattaaa
     4681 gttccaaaag gtaccttcat tgagccaagc gaacttaaaa atcttcatgc tagtgaagtt
     4741 ttaattatgt gtacaggtag tcaaggtgag tctatggcag cacttgctcg tatcgctaac
     4801 ggtactcatc gtcaagttac tttgcaacct ggtgatactg tgattttctc atcaagccca
     4861 atccctggta atacaacaag tgtcaataaa ttaatcaata ccattcaaga agctggtgtc
     4921 gatgttattc atggtaaaat taacaatatc catacctccg gccatggtgg tcaacaagaa
     4981 caaaaattaa tgttacgctt gattaaacca aagtatttca tgcccgtaca cggtgagtat
     5041 cgcatgcaaa aagtccatgc tggtttagca gttgacacag gtattcctaa agagaatatc
     5101 ttcattatgg aaaacggtga tgttttagct ttaacgagcg actctgctcg cattgctgga
     5161 catttcaatg ctcaagatat ctacgttgat gggaatggta ttggcgatat tggtgcggca
     5221 gttctacgtg atcgccacga cttatctgaa gatggtgttg tccttgccgt tgctaccgtt
     5281 gatttcgatt caaaaatgat tttagctgga ccagacatcc ttagccgagg ctttatctat
     5341 atgagagaat ctggggacct tattcgcgaa agccaacata tcttattcaa tgctattcgt
     5401 attgcactga aaaataaaga tgcaagtatc caatcagtaa atggagctat tgttaatgcc
     5461 ttacgaccat tcttatatga aaaaactgag cgtgagccta tcattattcc tatggtcctt
     5521 acccctgata aataaacaaa agaacggaaa ataccttttc cgttcttttt aattgcctat
     5581 attagcaaaa taagttaata tagcaaatta cttctgggat aactatagaa tataaaagaa
     5641 ctccatccaa acagtatcgt acttttatac cgtctatgtg aggagttctt tatagattaa
     5701 ttcggataaa tgtagctaac tacccctggt gtcgctgtat tttgtggatc aaaccaacca
     5761 cgataattat caatccattg atgacctgca taattagatt ctaaaacctg aattttatta
     5821 gtgacaggat ctacagctgt aacataagcc acatgtccat aactaccatc attccaacat
     5881 acgatagaac ccacgcgagg ttgtgttcct actgtataac cagctgatgt ggcgctcgca
     5941 gcccaatcac caccgtttcc ccaccagtta cctgcccatg ttgccatctc ctttacaccc
     6001 catgtgcatt gacctagtgg aaacgtatta ctagctggat gtgcttgagc aatttcctcc
     6061 gcacttgcta ggtagtctac atctactgca tagttatatg tattcgcatc tgcatttgtt
     6121 gaagcaaatg aaaaagcgga caccaaagcc actgttccaa gcattgtttt agactttaat
     6181 tgttttatgt tcatgttttc ctccatctga tgactcctag tttatcaaat ccatataaca
     6241 gacaaatgaa aacctgtaac aagttttagg caatttttta caataattta caaaaaaact
     6301 agttttagac tttacaaaat aaagcctaaa actagttata aacattctta tgattaatat
     6361 aaatctaggt aattatcaat ttcccattgt gaaacataag tcgcataact tgcccattca
     6421 atacgcttag catctaagaa attagtatag atatgagttc ctagggccgc ttttaccact
     6481 tcatcttctt caagtgcttt aagagcatta tgaagagtag atggtaaatc aacaatacct
     6541 gcttgacgac gttcttctac agtcatagca tagatatttg tctcaattgg ttctggtgct
     6601 tcaattttat tctcgattcc ctcaagtcct gatcccaaaa gtactgataa agcaagataa
     6661 ggatttgctg ttgggtcaac cgagcgcaat tctaggcgtg ttcccatacc tcgagatgca
     6721 ggtacacgaa taagtggcga acgattgcga cctgcccaag caatgtaaac tggtgcttcg
     6781 tatcctggta ccaaacgttt ataagaatta acagttggat taatgatagc tgtgtaattg
     6841 taagcgtgtt ccattaaccc acctaaaaag tagtaggcat cctctgataa ttgcattcca
     6901 cgtggatctt ctggatcaaa gaaagcattg ttcccttcat tatcaaaaag agacatatta
     6961 caatgcatac ctgaaccatt gataccaaac ttaggttttg ccataaaagt agcatataaa
     7021 ccatgcttac gagcaattgt tttaacaacc agtttaaata attgaatgtt gtcacaggct
     7081 tttaaaacat catcatattt aaagtcaatc tcatgctgtc caactgctac ttcatgatga
     7141 ctagcttcaa cttcaaagcc catttgtgtt aagacattaa caatttcacg acgtgtatta
     7201 tctgctaagt ctgttggagc taagtcaaag tagcctcctt tgtcattaac atcaagtgtt
     7261 gggttaccat tttcatccat tttaaatagg aaaaattcag gttctggtcc taagttaaat
     7321 gacttatagc ccatctcttg catacgtttc atattacgct taagatttcc acgtggatct
     7381 ccggcaaaag gttctccttc tgctgtatag atatcacaga ttagacctgc aacagctcca
     7441 ttttcatctc cccaaggaaa aacaatccaa gtatctaaat caggatataa atacatatca
     7501 gattcattga tacgaacaaa gccctcaata gaagatccat caaacattgc tttatttgat
     7561 aaaactttgt ctaattgttc atctgtagcc ggaatttcga catttttcat tactcctaag
     7621 atatctgtga acatcaaacg gaggaaggta acattttttt ccttaacttc gcgacgaata
     7681 tcttctgctg tgatagtcat atagtctcct taatatctag cttgactgca tgtttacaaa
     7741 tgcatatttc caatgtgctg tgaaggactt gaaaaacgcc cttgattacg gagctcatct
     7801 tgtaaaagtc gacgaacctc ggcatctgat aatgactttt gtttctcttt ggacttatgt
     7861 tgtctttgca tgtattcatt tttaatatca gaaatatgca aaccatcgct aatgaagtct
     7921 tttatttcca gtaagcggtc catatcattc agtgaaaaca tgcggcggtt accttcagta
     7981 cgttcaggtg tgattaaccc ttgatcctca taatagcgaa tttgtctggc agtcaaatct
     8041 gtcaacttca taacagttcc aattgggaat acagccattg tacgcctaag ttctctttct
     8101 ttcatgatgg cctcctttca ttaaaccatt ataaacttta aaaagtttga tgtcaactct
     8161 catgtgagat tttatgacat caaactataa tttgttattt tttagttttt tccgaagtat
     8221 ttcaatatct gtattaacca tcattgcaat aaaaacacca caaaaagttt caaaaatacg
     8281 tgcaaaaaca tatagaattg tctctccgct tgggattgat aaagttataa taagtaatgc
     8341 ggctgtcccc ccaataatac ctgatttatt gttacaagca acattaatca tgatacataa
     8401 catggtacaa ataggaacaa ttaataatgt aacccaaaaa gcttgatgaa atatctcatt
     8461 gaatgcaaaa aatactaagg agagtaagcc acctatacta ttaccaataa ttctagaaaa
     8521 accaaagtgc acgcttttat caaagtcttc tcttaaacta aatacagcag ttagagcacc
     8581 aatttgaagc cctttccaac caaataaatg aaaaaccaat aaaactaaaa agaccgataa
     8641 gcctgtttta aatgttctca ttcccaattt aaacttttta atatcaaatt tgtaattaga
     8701 taataatttt tttaacgttt tcatacctat cccttcacta tat
//
LOCUS       DBJYSH010000086         8587 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.086, whole genome shotgun sequence.
ACCESSION   DBJYSH010000086 DBJYSH010000000
VERSION     DBJYSH010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 8587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8587
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.086"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            6..680
                     /locus_tag="ACUBV3_001550"
     CDS             6..680
                     /locus_tag="ACUBV3_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HHU3848043.1"
                     /translation="MDKEKLDYWKTIITFLHNVLGDNYEIVLHVVDENDIYIGELVNS
                     HISGRTISSPLTTFALDLIKNKVYKEKDFVTNYKAIVSPLNKEVRGSTFFIKNAQNEL
                     EGMLCINLDISAYQNIALDILDLVNLNVNKILPKSPQKISLPQQEEPVEVLSGNIQDI
                     ISEIVDPSLLNQNIHLSQEVKVEIVSKLHEKGVFQLKGAVSKVAEVLNISEPSVYRYL
                     KKIETH"
     gene            850..3102
                     /gene="gshAB"
                     /locus_tag="ACUBV3_001551"
     CDS             850..3102
                     /gene="gshAB"
                     /locus_tag="ACUBV3_001551"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HHU3848044.1"
                     /translation="MIIDRLLQRSHSHLPILQATFGLERESLRIHQPTQRVAQTPHPK
                     TLGSRNYHPYIQTDYSEPQLELITPIAKDSQEAIRFLKAISDVAGRSINHDEYLWPLS
                     MPPKVREEDIQIAQLEDAFEYDYRKYLEKTYGKLIQSISGIHYNLGLGQELLTSLFEL
                     SQADNAIDFQNQLYMKLSQNFLRYRWLLTYLYGASPVAEEDFLDQKLNNPVRSLRNSH
                     LGYVNHKDIRISYTSLKDYVNDLENAVKSGQLIAEKEFYSPVRLRGSKACRNYLEKGI
                     TYLEFRTFDLNPFSPIGITQETVDTVHLFLLALLWIDSSSHIDQDIKEANRLNDLIAL
                     SHPLEKLPNQAPVSDLVDAMQSVIQHFNLSPYYQDLLESVKRQIQSPELTVAGQLLEM
                     IEGLSLETFGQRQGQIYHDYAWEAPYALKGYETMELSTQLLLFDVIQKGVNFEVLDEQ
                     DQFLKLWHNSHIEYVKNGNMTSKDNYIVPLAMANKVVTKKILDEKHFPTPFGDEFTDR
                     KEALNYFSQIQDKPIVVKPKSTNFGLGISIFKTSANLASYEKAIDIAFTEDSAILVEE
                     YIEGTEYRFFVLEGDCIAVLLRVAANVVGDGIHTISQLVKLKNQNPLRGYDHRSPLEV
                     IELGEVEQLMLEQQGYTVNSIPPEGTKIELRRNSNISTGGDSIDVTNTMDPTYKQLAA
                     EMAEAMGAWVCGVDLIIPNATQAYSKDKKNATCIELNFNPLMYMHTYCQEGPGQSITP
                     RILAKLFPEL"
     gene            3226..4044
                     /locus_tag="ACUBV3_001552"
     CDS             3226..4044
                     /locus_tag="ACUBV3_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3848045.1"
                     /translation="MVKKRYSKNSHNLITLLGIVVLASLISDFWSEVIATLLIIGGGY
                     CAYYVYDKKRLKRFTSNQRIEALKSDIKETDQDIRHLEILKKDNRSKEYIKLAHQILP
                     QLDLIRNEANQLQKAIEPNIYKRITKKANTFSNEINEQLIKLHASPELEPISDQEDEM
                     IRIAPELKPFYHNIQDDHFAILKKIEEADNKAELAAIHQANMKRFTDVLAGYIRIKQS
                     PKNFNNAKERLEQALQAIKKFNLDLDETLRQLNESDMKDFDVSLRMMQDERNSK"
     gene            4041..5297
                     /locus_tag="ACUBV3_001553"
     CDS             4041..5297
                     /locus_tag="ACUBV3_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HHU3848046.1"
                     /translation="MSDTFNFDIDQIADNAITKTDKTTEIISNQTTSQTGQIAFFEKL
                     TPAQKSAISEKTPALVDTFVGDQNALLDFGQSAVEGVNTTVNHILSEQKKIQIPQVDD
                     LLKNANRELNGFIAKYKDATPAELEKKPNLIQKLFKQSKTSLQEFYFDSQNIEQKMDM
                     MAANVVKQEDTLARNIVSAEMLIEDNTKSIENLVGVIAFIESSQAEAANRASHLQQEI
                     LALDSQTSEYQIKSNQLARMTEVINTLEQQHTEYVSRLYVAWATTPQMRNLVKVSSDM
                     RQKLGMLRRNTIPTMKLSIAQLGMMQQSVKSGVTADAIVNANNAALQMLAETSKEAIP
                     MLEKTAQSPTVSIKSVTALAESLVAQNNGIIAAIDKGRKERAQLESAVIKSAETINDS
                     VKIRDKKIVEALLNEGKSTQEKVDES"
     gene            5437..6312
                     /gene="hslO"
                     /locus_tag="ACUBV3_001554"
     CDS             5437..6312
                     /gene="hslO"
                     /locus_tag="ACUBV3_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HHU3848047.1"
                     /translation="MDKIIKSISTSGSFRAYVLDCTETVRTAQEKHQTLSSSTVALGR
                     TLIANQILAANQKGNSKVTVKVIGDSSFGHIISVADTKGNVKGYIQNTGVDIKKTATG
                     EVLVGPFMGNGHFVVITDYATGQPYTSTTPLITGEIGEDFAYYLTESEQTPSAVGLNV
                     LLDDEDKVKVAGGFMLQVLPGASDEEISRYEKRIQEMPSISSLLESENHIESLLSAIY
                     GEDDYKRLSEDSLAFYCDCSKERFEAALLTLGTKELQAMKDEDKGVEITCQICNQTYY
                     FTEEDLEKIINDSIK"
     gene            6296..7273
                     /gene="dusB"
                     /locus_tag="ACUBV3_001555"
     CDS             6296..7273
                     /gene="dusB"
                     /locus_tag="ACUBV3_001555"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HHU3848048.1"
                     /translation="MTQLNSSFMIGKVEIPHRTVLAPMAGITNSAFRTIAKEFGAGLV
                     VMEMISEKGLLYNNEKTLHMLHIDENEHPMSIQLFGGDAEGLKRAADFIQSNTKADIV
                     DINMGCPVNKVVKNEAGAKWLRDPEKIYHIVKEVTSVLDIPLTVKMRTGWSDSSNAIE
                     NALAAESAGVSALAMHGRTREQMYTGTCDHETLGKVAKAVTSIPFIANGDIRTVHDAK
                     FMIEEIGADAIMVGRGARSNPYIFTQINHFFETGEILPDLPFEKMLDVAEDHLTRLVN
                     LKGETIAVREFRGLAPHYLRGKSGAAKIRGAVSRAETLAEVQELFAGLR"
     gene            7394..8035
                     /locus_tag="ACUBV3_001556"
     CDS             7394..8035
                     /locus_tag="ACUBV3_001556"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019775268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HHU3848049.1"
                     /translation="MLIVLAGTIGAGKSSLAAALGQHLGTDVFYEAVDNNPVLDLYYQ
                     DPQKYAFLLQIFFLNKRFQSIKEAYKANNNVLDRSIFEDELFLTLNYKNGNVTKTELD
                     IYKELLANMLEELEGMPKKRPDLLVYIDVSFDKMLERIDKRGRSFEQVDSNPELYDYY
                     KQVHSEYPEWYENYDVSPKIRIDGNKLDFVKNPEDLQHVLDTIDSELQKLDLL"
     gene            8049..8540
                     /locus_tag="ACUBV3_001557"
     CDS             8049..8540
                     /locus_tag="ACUBV3_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HHU3848050.1"
                     /translation="MIIRPVLPSDAKELLAIYAPYVADTTITFEYDIPSISEFTNRIK
                     NISKSFPYLVAEENGKILGYAYASTYYARAAYDWTCELSIYLDKDARSKKIGSQLYDT
                     LEKELVNQGYVNLLACISLPNDISIAFHKKRGFNQVAHFPKIGFKFEQWHDIVWLQKR
                     LKS"
BASE COUNT         2938 a         1542 c         1527 g         2580 t
ORIGIN      
        1 acgatatgga taaagaaaaa ttagactatt ggaaaacgat aataacgttt ctccataatg
       61 ttttaggtga taattacgaa attgttttgc atgtagtaga tgaaaatgat atctatattg
      121 gtgagttggt taatagtcac attagtggac gaaccataag ttcacctcta acgactttcg
      181 cccttgattt aatcaagaat aaagtttata aggaaaaaga ttttgttact aactataagg
      241 cgattgtcag ccctctcaat aaagaagttc gtggatctac tttcttcatc aagaatgctc
      301 aaaatgaact tgaagggatg ttatgtatca accttgatat ttctgcatac caaaatattg
      361 ctttggatat tctagactta gttaatttaa atgttaacaa aatactacct aagtcccctc
      421 aaaaaatttc tctccctcaa caggaggaac ctgtagaagt tctatcaggc aatattcagg
      481 atattattag tgagattgtt gatcctagtc ttttaaatca gaatattcat ttaagtcaag
      541 aagttaaggt agaaattgtt tctaaacttc acgaaaaagg tgtattccaa ttaaaaggtg
      601 ccgtttcaaa ggtcgctgaa gttctcaaca tctccgaacc aagcgtctat cgttatctta
      661 aaaaaattga aactcattaa ctctaaaact ctttttaact tatagattaa taagattgga
      721 ctcaaaagtt caatctttta ttttttatga caccggtata agattatttt agttttctgt
      781 gccgtaaaac ttagttttta ttattttttt gataaactag tatcatctgt tactaggagg
      841 aatgttgtcg tgattatcga tcgactgtta caaagaagcc actctcatct accgattcta
      901 caagctacat ttggcttaga gagagaaagc cttcgtattc accaacccac tcaaagggtt
      961 gctcaaacac cccatcccaa aacattaggg agtcgtaact atcatcctta tatccaaacg
     1021 gattatagtg aacctcaatt agaactcatt acacctattg caaaggatag ccaagaagcc
     1081 attcgatttt tgaaagctat aagtgatgtt gctggacgct ctatcaacca cgatgaatac
     1141 ttatggccgc tttctatgcc acccaaagtg agggaagaag acatacagat tgcccaatta
     1201 gaggatgctt ttgaatatga ttatcgcaaa tatttggaga agacttatgg aaaactaata
     1261 caatccatct ctggaattca ctataatctc ggtttaggtc aagagttatt aacttctctc
     1321 tttgaattga gtcaagcaga taatgctatt gattttcaga accagcttta catgaaattg
     1381 tctcaaaatt ttttacgtta ccgttggtta ctaacttatc tatacggagc gagcccagta
     1441 gctgaagaag actttttaga tcagaaactg aataaccctg tccggtcact tagaaatagt
     1501 cacttaggtt atgtgaatca taaagatatt cgtatttctt atactagctt gaaggattat
     1561 gtcaacgatc tagaaaatgc tgtaaaaagt gggcaattga ttgctgagaa agaattttat
     1621 tcacctgttc gtctccgtgg tagtaaggcc tgtcgtaatt atttagaaaa aggaattaca
     1681 tatttagagt ttcgcacttt tgatctcaac ccatttagcc caatcggtat aacgcaggaa
     1741 actgttgata ctgttcacct tttcttattg gcgcttttgt ggatagattc tagtagtcat
     1801 attgaccaag atatcaagga agccaataga ttaaatgacc ttatagcact tagtcatcca
     1861 ttagaaaaat tacctaatca agcaccagtt tctgacttag tggatgctat gcaatctgtt
     1921 atccaacatt ttaacttatc accttactat caagacctat tggaatctgt aaaaaggcaa
     1981 attcagtcac ctgaattaac ggtagctggt caacttttag aaatgattga aggactttcc
     2041 ttagaaacat ttggacaaag acaaggacaa atttatcacg attatgcttg ggaagctccc
     2101 tacgccttaa agggatatga aacgatggaa ctttctaccc aattgctact atttgacgtt
     2161 atccaaaaag gagtcaactt cgaagtgctg gatgaacaag atcaattcct aaaattatgg
     2221 cacaatagtc atattgagta cgttaaaaat ggtaatatga cctcaaaaga taactatatt
     2281 gtaccgctcg ctatggctaa caaagttgtt acaaaaaaaa tcctagatga aaagcatttc
     2341 ccaactcctt ttggagatga atttactgac cgtaaagagg cacttaacta tttttcacaa
     2401 atccaagata aaccaatcgt cgttaagcca aaatctacaa actttggttt aggcatttct
     2461 atttttaaaa catcagctaa cttagcttct tatgaaaaag caattgacat cgcttttacc
     2521 gaagatagtg ctattcttgt ggaagaatat attgagggta ccgaataccg tttcttcgtc
     2581 cttgaggggg actgtattgc tgtattgctt agggtagcag ctaatgtcgt tggagatggt
     2641 attcatacta ttagtcagct agttaaactt aaaaaccaaa accctctcag aggctatgat
     2701 catcgctctc ctctagaagt tattgagctc ggagaagtag aacagttaat gttggaacaa
     2761 caaggataca ctgttaacag tatccctcca gaaggaacaa aaatagaact tcgccgtaat
     2821 tctaatattt ccactggcgg tgactctata gatgtaacaa atacaatgga tcctacatat
     2881 aaacaactag cagctgagat ggcagaggct atgggggctt gggtttgtgg agttgattta
     2941 attattccta atgccactca agcatactca aaagataaga aaaatgctac ttgcattgaa
     3001 ctaaacttta acccattgat gtatatgcac acctactgtc aagaggggcc tggtcaatct
     3061 attacacctc gaattttggc taaactgttc ccagaattat aactaaaaag agttccttag
     3121 gctaagctta aggaattttt ggttaaaata aagttatccc ttgtcatcac tagttcattt
     3181 aagataaaat attaacaact acagaaagtt aggttaaata ttattatggt taaaaaacgc
     3241 tattccaaaa attctcataa tctaataaca ttacttggta ttgttgttct tgcttccctg
     3301 atttcagact tttggagtga ggtgattgct actctgttaa ttattggtgg tggttattgt
     3361 gcctattacg tttatgataa aaagcgtctt aaacgtttta catcaaacca acgtattgaa
     3421 gctttaaaat ccgatattaa agagactgac caagacatta gacacttgga aattttaaaa
     3481 aaagacaatc gttctaagga atatatcaaa ttagcacatc aaatattacc tcaattggac
     3541 ctaattcgta atgaagcaaa ccaactacaa aaagccattg agcctaatat ctataaacgt
     3601 atcaccaaaa aagcaaatac atttagtaat gagattaacg aacaactaat aaagctacat
     3661 gcctctccag aactagaacc tatttctgat caggaagatg aaatgatacg cattgctccg
     3721 gaattaaaac cgttctatca caatattcaa gatgaccact ttgctatctt aaaaaagatt
     3781 gaagaggcag ataacaaagc tgaattagca gcaattcatc aagccaatat gaaacgtttt
     3841 acagatgttc tggctggcta catcagaatc aaacaatctc ctaaaaattt taacaatgct
     3901 aaggaacgcc tagaacaagc attacaagca attaaaaaat ttaatttaga tttagatgaa
     3961 acgctgcgcc aattgaatga aagtgatatg aaagattttg acgttagcct aagaatgatg
     4021 caagacgaaa ggaacagtaa atgagcgata cctttaattt tgatattgac caaattgcag
     4081 acaatgctat cactaaaaca gataaaacaa cagaaattat ttccaaccag acaacaagcc
     4141 aaactgggca aattgccttt tttgaaaaac taacaccagc acaaaagtct gctatctctg
     4201 aaaaaacacc agctttggta gatacttttg tcggcgatca aaatgcgctc cttgattttg
     4261 gacaatccgc agtagaaggc gttaatacca ctgttaatca tatcttgtct gagcagaaaa
     4321 aaattcaaat tcctcaagtt gatgatttac taaaaaatgc taatcgcgaa ctaaatggat
     4381 ttattgccaa atataaagat gctactccgg cagaattaga gaaaaaacca aacttgattc
     4441 aaaaattatt caaacaaagc aagacctcgc tacaggaatt ttattttgac tcacaaaaca
     4501 tcgagcaaaa aatggatatg atggcagcga atgttgtcaa acaagaagat actttggcaa
     4561 gaaatatcgt ctctgctgaa atgctcattg aagataatac taaatctatt gaaaatttgg
     4621 ttggagttat tgcttttatt gaatcgagtc aagccgaggc tgctaatcgt gcaagccact
     4681 tacaacaaga aattctagca ttagatagcc aaacgtccga gtatcaaatt aaaagtaacc
     4741 aattagctcg aatgactgaa gttatcaata ccctcgaaca gcaacatact gaatatgtca
     4801 gccgtctcta cgttgcatgg gcaacaacac cacagatgcg aaacttggtc aaagtatcgt
     4861 cagatatgcg tcagaaactt ggcatgttac gtcgaaatac cattccaaca atgaaactct
     4921 caatcgctca gttaggcatg atgcaacaat ctgtcaaatc cggtgtcact gctgatgcta
     4981 ttgtcaacgc taataatgca gcattgcaga tgctggctga aactagtaaa gaagcgattc
     5041 cgatgttaga gaagaccgca caaagcccca ctgtttctat taaatctgtc actgcattag
     5101 ctgaaagctt agtggctcaa aataatggta ttatcgctgc catagacaaa ggacgtaagg
     5161 aacgtgccca attggaatct gctgttatta aatcggctga aacaatcaat gattctgtca
     5221 aaattcgtga taaaaaaata gttgaagcct tactcaacga aggtaaatct acccaagaaa
     5281 aagttgatga gtcttaactt actatcatat ccactaaata aaaacgatac tgtaaaacac
     5341 agtatcgttt ttatatcatt aatttatacc gttttcatct atattttcct gactatctcc
     5401 aatgcaaaat aagcctttta tgctataata tgacttatgg ataaaattat aaaatcaatt
     5461 tcaacttccg gttctttccg ggcttatgtc cttgattgta cagaaactgt aagaacagct
     5521 caagaaaaac atcagacact ttcaagttct actgtagctc ttgggagaac attaattgca
     5581 aatcaaattt tagcagcaaa tcaaaaagga aatagtaaag taactgtcaa ggttattgga
     5641 gatagctctt ttggtcatat tatttctgta gctgatacta aaggaaatgt taaaggttac
     5701 atccaaaata cgggtgttga tatcaagaaa acagctactg gagaggtctt agttggacct
     5761 ttcatgggta atggacactt tgtcgttatt accgattacg caactgggca accttacact
     5821 tcaacaactc cactaattac aggagaaatt ggagaagatt ttgcttatta tttaaccgaa
     5881 agtgagcaaa caccttctgc cgtcggttta aatgtcctct tagatgacga ggataaggtt
     5941 aaagttgcag gtggattcat gcttcaggtg ctcccaggtg cttctgacga agaaatttct
     6001 cgctatgaaa aacgcattca ggagatgcct tctatttcaa gtctgctaga gtctgaaaat
     6061 catatagaat ctctcctttc agctatttac ggagaagacg actacaagcg cttatcggaa
     6121 gactctttag ccttctactg tgactgttca aaagaacgtt ttgaagccgc tctattgact
     6181 ttaggaacta aagaattgca agccatgaag gatgaagata aaggtgttga aattacttgt
     6241 caaatttgca accagactta ctatttcaca gaagaagatt tggagaagat aattaatgac
     6301 tcaattaaat agttcattta tgattgggaa ggttgaaatt cctcatcgta ccgtactagc
     6361 accgatggca ggtattacca attctgcttt tcgtactatc gctaaagaat ttggtgctgg
     6421 tctcgttgtt atggaaatga tttctgagaa agggttactt tataacaacg aaaagacctt
     6481 acatatgctc catatcgatg aaaatgaaca tcctatgtca attcaactat ttggtggcga
     6541 tgcagaaggc ttaaaacgtg cagctgactt tatccaaagt aatacgaaag cagatatcgt
     6601 agacatcaat atgggatgtc cagttaacaa agttgttaaa aacgaagctg gagctaaatg
     6661 gttacgtgac cctgaaaaaa tttatcatat tgttaaagag gtaacgtctg tacttgacat
     6721 tccattaaca gtaaaaatgc gtacaggatg gtctgactca tctaatgcta tagaaaatgc
     6781 actcgctgcc gaatctgctg gtgtatctgc acttgctatg cacggtcgaa cgcgtgaaca
     6841 aatgtatact ggcacgtgtg accatgaaac acttggcaag gtcgctaaag ctgttactag
     6901 tattcctttt attgctaatg gtgacattcg tactgttcac gatgccaaat ttatgattga
     6961 agagattgga gctgacgcta taatggttgg acgtggtgct cgtagcaatc cttacatctt
     7021 tacacaaatt aatcatttct ttgaaacagg cgaaatcctt cctgacctac catttgagaa
     7081 gatgttagat gttgccgagg atcatctcac tcgactagtt aatttaaaag gtgaaactat
     7141 tgccgttcgt gaatttcgag ggttagcacc tcattatctg cgtggaaaat caggcgcagc
     7201 aaaaattaga ggagctgttt cacgtgcaga aacactagca gaagttcaag aactttttgc
     7261 aggtttacgc taaatcaaaa acagtccttt gactgttttt tcctatctct gtacaaattt
     7321 agcataaatc attgtgtaat gaaaactcaa aagtataaca aattatttta gcataacaga
     7381 attaaggaga attatgttga ttgttttagc tggaaccatt ggagcaggta aaagctccct
     7441 tgcagctgct ctaggacaac acctagggac agatgttttc tatgaggcgg ttgataataa
     7501 cccagtttta gatctttact atcaagatcc ccaaaaatat gcttttttac tacaaatatt
     7561 ctttttaaat aaacgattcc aatctataaa agaagcctat aaggctaaca acaatgtttt
     7621 ggatcgttct atttttgaag atgaactatt cctcactcta aattataaga atggaaatgt
     7681 gactaaaaca gagctagata tctataaaga acttcttgca aatatgttgg aagaattaga
     7741 aggtatgcct aagaagagac ctgatttgct agtctacatt gatgtttcat ttgataaaat
     7801 gttagaacgt attgacaaac gaggacgcag ctttgagcaa gttgattcta acccagagtt
     7861 atacgactac tacaaacaag tccattctga atatccagaa tggtatgaaa attacgacgt
     7921 ttcacctaaa attcggatcg atggtaacaa acttgatttc gtcaaaaatc cagaggatct
     7981 tcaacacgtc cttgacacta ttgattccga attgcaaaaa cttgatttac tataattagg
     8041 tgatatctat gattataaga cctgtcctac cctcagatgc taaagaatta cttgctatct
     8101 atgctcccta cgttgctgac actacaataa cgtttgaata cgatattcct agtatatcgg
     8161 aattcactaa tcgtatcaaa aatatcagca aatctttccc atatttagtt gctgaagaaa
     8221 atgggaaaat attaggttat gcatatgctt caacttacta cgctagagct gcctatgact
     8281 ggacttgcga gctttctatt taccttgata aagatgcacg tagcaaaaaa attggtagcc
     8341 aactatatga tacattggag aaagaattag ttaaccaagg gtatgttaat ctactcgctt
     8401 gtatcagttt accaaatgat ataagcattg cattccacaa aaaaagagga tttaatcaag
     8461 tcgcacattt cccaaaaatc ggatttaaat ttgaacaatg gcatgacatt gtgtggttac
     8521 aaaagcgatt gaaatcttaa ggtaatcttt agatttattt taagttagca aaagattagg
     8581 atattat
//
LOCUS       DBJYSH010000087         8414 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.087, whole genome shotgun sequence.
ACCESSION   DBJYSH010000087 DBJYSH010000000
VERSION     DBJYSH010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 8414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8414
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.087"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            <1..823
                     /locus_tag="ACUBV3_001558"
     CDS             <1..823
                     /locus_tag="ACUBV3_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 52"
                     /protein_id="HHU3848051.1"
                     /translation="NIDDLCKRTNKEGILCFKLDETSSTPTTVDTITIDLIIETNGTI
                     YLFNDDTSVARQIVRAQKNYHLIEDGYNCFQAKLFLGGSVVKRVIKTYLFKKYVPYGF
                     SKYCLSIEVNSLVGLPHDIRSKKYKELPRKKLFDSLNKEQKSLIFKIFKTKPLTITPK
                     SVLLLTQPLAQDKCYKTPTERFQSIQEQYDYFDDIVQEYRTLGYNVYLKVHPRDVVDY
                     SKLRVELLPSNIPMEIIELMLTGRFECGITHSSTALDFLTCVDKKITLVDLKDIK"
     gene            820..2220
                     /locus_tag="ACUBV3_001559"
     CDS             820..2220
                     /locus_tag="ACUBV3_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HHU3848052.1"
                     /translation="MNTKKLLQSGFIYTLGNLLVQGLAFITLPIYTRVISTEVYGQYS
                     LYVAWMGIITLFIGLQTSGSLSSARVKYGEEFKSYSGSAFSVGNIWFLIVLLIAFLFR
                     SFLAPLVGFSESIFLLMVCQSYANYVVTFFGQYFIQQQRSLANLILSLANAVSSVALS
                     LFLIFHWSDDFLSRVLGAFVPTIITGIVAFAYIYYHSKSFYNPKYFRFIVTVSIPLIF
                     HLLGHQLLNQLDRIMLARLYSTKEVAMYSFGYSLGMIIQIVLNSINMAWIPWFFDARK
                     EKLLQLSTYVSRYLYLGVFLTLGYLTVFPELAQIMGGDKYSSSVQFISLIIVSYFLVF
                     LYTFPVNIQFFYANTTWIPIGTLLAAGVNWLLNLVLIPHYAAYGAAMATIISYLALLI
                     FHHIVSKVKYHYSDVSVRQYIILSGIVFSYAMLMNMFLDNIVIRWSLGIIILIVYSIV
                     FQKVILDLLSKKRRRR"
     gene            2220..3239
                     /gene="neuB"
                     /locus_tag="ACUBV3_001560"
     CDS             2220..3239
                     /gene="neuB"
                     /locus_tag="ACUBV3_001560"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HHU3848053.1"
                     /translation="MVYIIAEIGCNHNGDINLAKKMVDVAVSCGVDAVKFQTFKAEKL
                     ISKFAPKAEYQKATTGTADSQLEMTKRLELSFEEYLEMRDYAISKGVETFSTPFDEES
                     LEFLISTDMPIYKIPSGEITNLPYLEKIGKQQKKVILSTGMAVMEEIHQAVNILRQNG
                     TTDISILHCTTEYPTPYPSLNLNVIHTLKDEFKDLTIGYSDHSIGSEVPIAAAAMGAE
                     VIEKHFTLDTNMEGPDHKASATPDILAALVKGVRIVEQALGRFEKIPDPVEEKNKIVA
                     RKSVVALKPIKKGDIYSIENITVKRPGNGISPMNWYDILGQEAQDDFEEDEVIRDSRF
                     ENQLP"
     gene            3322..4476
                     /gene="neuC"
                     /locus_tag="ACUBV3_001561"
     CDS             3322..4476
                     /gene="neuC"
                     /locus_tag="ACUBV3_001561"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0006047 - UDP-N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HHU3848054.1"
                     /translation="MKKICLVTGSRAEYGIMKPLIQRLSKDKEINLQIIATAMHLEEK
                     YGYTYRQIEEDGFDIAYKVPLHLYDTDRRTVSTAMAHLQLGLTKIFDKEDYDLVIILG
                     DRYEMLPVVNVALIYNVPVCHLHGGETSLGNFDEYIRHAITKMSHLHLVSTEDFRQRV
                     IQMGEQPQFVINTGALGVENALSIPPLTKEAIEKQLGIALEESYFVVLYHPVTFEQGK
                     SAGEQMKAVLSALSKFGVQCLFIGSNSDTGSDDIAKAINTYLINHENSYCFASLSTQL
                     YHSLIRHSLGLIGNSSSGLIEVPSLMKPTLNIGDRQKGRLHGESVVSVPVETSSVLEG
                     LSKLNEVTNFDNPYYKENASSIAYEAIKLYLKDEPSISQPFYDLKENNLK"
     gene            4473..5102
                     /locus_tag="ACUBV3_001562"
     CDS             4473..5102
                     /locus_tag="ACUBV3_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HHU3848055.1"
                     /translation="MKKKLAFLGAGSHADAIAPVIDPLVYDFVGFFDDKDITEHDGYP
                     VLGKLYDVLPYLEDGSIDAVFITIGDNAKRKELFEYVAKDYYDFIINIISPNALVLTP
                     DSICGRGIFIGFGAFIGSKVKLFDNNVVNTGALIEHHTVVESHCNIAPNATINGLCYI
                     REEVYVGSASVIIQTLDISSCTTIGAGAVVVKDIIEPGTYVGVPAKKIK"
     gene            5113..6354
                     /gene="neuA"
                     /locus_tag="ACUBV3_001563"
     CDS             5113..6354
                     /gene="neuA"
                     /locus_tag="ACUBV3_001563"
                     /EC_number="2.7.7.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000802346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acylneuraminate cytidylyltransferase"
                     /protein_id="HHU3848056.1"
                     /translation="MKPICIIPARSGSKGLPDKNMLFLAGKPMIFHTIDAAIESGMFD
                     KKDIFVSTDSELYREICLERGISVVMRKPELSTDQATSYDMLKDFLSDYEDNQEFVLL
                     QVTSPLRKSWHIKEAMEYYSSHDVDNVVSFSEVEKHPSLFTTLSDEGYAIDMVGADKG
                     YRRQDLQPLYYPNGAIFISNKETYLSEKSFFTSKTYAYQMAKEFSLDVDTRDDFIHVI
                     GHLFFDYAIREKENKVFYKEGYSRLFNREASKIILGDSKTISISLESYHNYSQGGVTL
                     ATMLENLPNFLTANVTEAFVSIGVNDLITGYSVEEIFSNFQKLYSLLAENKIKMRLTT
                     IAYTLFRETVNNADIEKINQWLTEFCYQNQIPLLDINRFLSKDGNLNYHLTSDGLHFT
                     QEANDLLQSQYQLFVDEVKTL"
     gene            6468..6953
                     /locus_tag="ACUBV3_001564"
     CDS             6468..6953
                     /locus_tag="ACUBV3_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3848057.1"
                     /translation="MRETETHDHQALIQKLLVSIHYLTLFRDEIILVEKTPSLLGKHF
                     SIAIVQNELGEILSKIEALSKQKKLIRSIYWYDESSFKVMNKALAIVEEWIKGLDNLL
                     EFCQSQTVFQAILGDERAHVFGILIDVYTSLNIINTSLKEENSRPVSLSDLALGLKEE
                     D"
     gene            7052..7705
                     /locus_tag="ACUBV3_001565"
     CDS             7052..7705
                     /locus_tag="ACUBV3_001565"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HHU3848058.1"
                     /translation="MKHSSWHDLIKRELPNHYYNKINTFMDAVYESGIVYPPRDKVFN
                     AIQITPLENVKVVIIGQDPYHGPQQAQGLSFSVPDNLPAPPSLQNILKELAEDIGSRS
                     HHDLTSWAQQGVLLLNACLTVPEHQANGHAGLIWEPFTDAVIKVVNQKETPVVFILWG
                     GYARKKKSLIDNPIHHIIESPHPSPLSAYRGFFGSRPFSRTNHFLEEEGINEIDWLN"
     gene            complement(7771..8409)
                     /gene="plsY"
                     /locus_tag="ACUBV3_001566"
     CDS             complement(7771..8409)
                     /gene="plsY"
                     /locus_tag="ACUBV3_001566"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HHU3848059.1"
                     /translation="MNIIIMIIIAYLLGSIQTGLWIGKYFYQVNLRQHGSGNTGTTNT
                     FRILGVKAGIVTLTIDILKGTLATLIPIILGITTVSPFFIGFFAIIGHTFPIFAQFKG
                     GKAVATSAGVLLGFAPSFFLYLLVIFLLTLYLFSMISLSSITVAVVGILSVLIFPLVG
                     FILTDYDWIFTTVVILMALTIIIRHQDNIKRIRKRQENLVPFGLNLSKQKNK"
BASE COUNT         2804 a         1266 c         1538 g         2806 t
ORIGIN      
        1 taatattgat gatttatgta aacgtaccaa taaagaaggt attctatgtt ttaaattaga
       61 tgaaacttcg agtactccaa ctactgtaga tacaataaca attgacttaa ttatagaaac
      121 taatggaact atatatttat ttaatgatga tactagtgta gctcggcaaa ttgtacgcgc
      181 tcagaagaat tatcatctaa ttgaagatgg ttataactgt tttcaagcca agttattctt
      241 gggtggttct gtcgtaaaac gagtaattaa aacttactta tttaaaaagt atgttccgta
      301 tggtttttca aaatattgtc tctcaattga ggttaattct ttagtggggt tacctcatga
      361 cataaggagt aagaaatata aagaactacc gagaaaaaaa ttatttgata gtcttaacaa
      421 agaacaaaaa tcactgattt tcaaaatatt taaaacaaaa ccattaacta taactccaaa
      481 gtcagtatta ttgttgacac agccacttgc acaagataaa tgttataaaa cacctacaga
      541 gaggtttcaa agtattcaag agcaatacga ttattttgac gatattgtcc aggaatatag
      601 aacgttaggg tataacgttt atttaaaagt tcatcctaga gatgtagtag attattccaa
      661 attgcgggta gagctattac catcaaatat ccctatggaa attatagagt tgatgttaac
      721 aggtcggttc gaatgtggga taacacattc gtccaccgcg ctggattttt taacttgtgt
      781 tgataaaaaa ataactttag tggatctgaa ggatattaaa tgaatactaa aaaattactt
      841 caatctggtt ttatttatac actcgggaat ttattggtgc aaggattagc ctttatcaca
      901 cttcccatct acactcgagt catctcaaca gaagtttatg gccaatatag tttatatgtt
      961 gcttggatgg gaattattac tctgtttatt ggtcttcaga cgagcggttc tttaagttcg
     1021 gctcgagtca aatatggaga agagtttaaa agctactctg ggagtgcctt ttctgtaggt
     1081 aatatatggt ttcttattgt acttttgata gcttttctat ttagaagttt tcttgcacca
     1141 ttagttggtt tttctgaatc tattttttta ttaatggtgt gtcaaagtta cgctaactat
     1201 gtggtgactt tctttggtca gtattttata caacaacaga ggagcttggc taatttaata
     1261 ctatccttag ccaatgcagt ttcatctgtt gcactatctc tatttttaat ttttcattgg
     1321 tccgatgact ttttatctag ggttttggga gcttttgttc ctactataat aactggaata
     1381 gttgcctttg cttatattta ttatcatagc aaatcttttt acaatcctaa gtattttcgg
     1441 ttcattgtca ctgtgtctat tcctttgatt tttcatttgt taggacatca gttattaaat
     1501 caattagata ggattatgct agctcgctta tatagtacta aagaagttgc aatgtatagt
     1561 ttcggttatt cgcttggaat gattattcag attgttttga atagtattaa tatggcttgg
     1621 attccatggt tttttgatgc tagaaaagaa aaattactgc aactttcaac ttatgttagc
     1681 cgttatctct atttaggagt tttcttaaca ttaggttatt tgactgtttt tcctgagtta
     1741 gctcaaataa tgggaggaga caagtatagt agtagtgttc aatttatttc tttaattatt
     1801 gtcagttatt ttttagtctt tctctataca tttcctgtaa atatccaatt cttctatgct
     1861 aatacgacat ggattcctat tgggacatta ttagctgctg gtgtaaattg gttactaaac
     1921 cttgtattaa ttcctcatta tgcagcctat ggcgctgcta tggctacaat aatatcttat
     1981 ttagccttgt tgatttttca tcatattgtt tcgaaagtta aataccatta ttcagatgtc
     2041 tcagttaggc agtacatcat tttaagtggt atagtattta gttatgctat gttaatgaac
     2101 atgttcctag ataatatcgt tattagatgg agtttaggaa taattattct tatagtatat
     2161 agcattgttt ttcaaaaagt cattttagac ttattgagta aaaaaagaag gagaagataa
     2221 tggtttatat tattgcagag attggttgca atcataatgg agatattaat cttgcgaaaa
     2281 aaatggtaga tgttgccgtg tcttgtggtg ttgatgctgt taaatttcag acttttaaag
     2341 ctgagaaact tatttctaaa tttgctccca aagctgaata tcaaaaagca actacaggaa
     2401 cagcagacag tcaacttgag atgacgaaac gtttagagtt aagctttgaa gaatacttag
     2461 aaatgcgtga ttatgcaatt tcaaaaggtg tggagacctt ttcaacacct tttgatgaag
     2521 agtcattaga gttcttaatt tctacagaca tgccaattta caaaattcca tcaggagaaa
     2581 tcactaattt accttactta gaaaagattg gcaagcaaca aaagaaagtt attctttcga
     2641 cgggtatggc ggtaatggaa gagatccatc aagcggtgaa tattttacgt cagaatggta
     2701 caaccgatat ttctatttta cattgtacaa cagagtaccc aacaccttac ccctctctaa
     2761 atttaaacgt tattcatact ttgaaagatg aatttaaaga tttaacgata ggttattcgg
     2821 atcattcaat tggatcagaa gtacctattg cagcagcagc aatgggtgca gaagttattg
     2881 aaaaacactt tactttagat actaatatgg aaggtccgga tcataaagcc agtgcaacac
     2941 ctgatatttt agctgcttta gttaaagggg ttcgcattgt tgaacaagcc ttaggtagat
     3001 ttgaaaaaat cccagatcca gtagaagaaa aaaataagat tgttgctcgt aaatcagtcg
     3061 ttgctttaaa accaattaaa aaaggcgata tttattcaat agaaaatatt acggtgaagc
     3121 gcccaggtaa tggtatttct cctatgaact ggtatgacat cttgggacaa gaagcgcaag
     3181 atgatttcga agaggatgaa gttattcgtg attcacgctt tgaaaatcaa ttgccctagt
     3241 tataataaca cctaattata atcggcaaca gatgtattac acggctaaga ttatataatt
     3301 actatatata gaagggatac gatgaaaaaa atttgtttag taaccggatc gcgtgctgaa
     3361 tacggaataa tgaagccatt gattcaaaga ttatcaaaag ataaagaaat caacttacaa
     3421 attattgcaa cagcaatgca tctggaagaa aagtacggct atacttatcg tcaaattgaa
     3481 gaagacggtt ttgatattgc ttataaagtt cccttacatc tttatgatac tgacagaaga
     3541 actgtatcta ctgcaatggc gcatttacaa ctaggattga ccaaaatttt tgacaaggaa
     3601 gactatgatc tagtcatcat tttaggggat cgttatgaaa tgttaccagt tgtgaatgta
     3661 gcgttgattt ataatgtccc agtatgccac cttcatggag gggagacatc attaggcaat
     3721 tttgatgagt atattcgcca tgcaattact aagatgagtc acctacactt agtctctaca
     3781 gaggactttc gtcaacgtgt gattcagatg ggagaacaac ctcaatttgt aattaacaca
     3841 ggagctctcg gagtggaaaa tgctctatca atccctcctc taaccaaaga agcaatagaa
     3901 aagcaattag gtattgcact agaggaatcc tattttgttg ttctctatca tcctgttact
     3961 tttgaacaag gaaaaagtgc tggagagcag atgaaagcgg tactatcagc cctttctaaa
     4021 tttggtgtac agtgtctatt tataggatcc aattcagata ctggttctga tgatattgca
     4081 aaggctatca atacttattt gataaatcat gaaaatagtt attgctttgc ttctttatca
     4141 acccaacttt atcattcctt aattcgtcat tcactaggac tgattggtaa ttcgtcttct
     4201 ggtttaatag aagttccttc tttgatgaaa cctactctta atattggtga tcgccaaaaa
     4261 ggtaggctac atggagaaag tgtagtaagt gtaccagtcg aaacctcctc tgtattggag
     4321 ggattatcaa aattgaatga agtgaccaac tttgacaatc cctactacaa agagaatgct
     4381 tcatctattg catatgaagc aataaagctt tatttgaaag atgaaccttc aatttcccaa
     4441 ccattttatg atttaaagga gaataatcta aaatgaaaaa aaagttggca tttttaggcg
     4501 cagggagtca tgctgatgcg attgctccgg ttattgatcc tcttgtgtat gatttcgtag
     4561 gtttttttga tgataaagat attacggagc atgatggtta tcctgttctt ggaaaacttt
     4621 atgatgtgct accttacctt gaagatggct caatagatgc agtatttatt acaataggtg
     4681 ataatgctaa aaggaaagaa ctatttgaat atgtagcaaa ggattattat gactttatta
     4741 ttaacatcat tagtcccaat gctttagtat tgacaccaga tagtatttgt ggacgtggta
     4801 tctttattgg ttttggggct tttataggtt ctaaagtgaa gctgtttgat aacaacgttg
     4861 ttaatacagg agcgctcatt gaacatcata ctgttgtaga atcacactgt aatatagcac
     4921 ctaatgctac cataaatggt ctttgttata ttagagaaga agtttatgta ggtagtgcca
     4981 gtgttattat tcaaaccttg gatatttcat cgtgtacgac aattggagca ggagctgttg
     5041 ttgtaaaaga tattatagaa cctggaacct atgtaggggt tccagcaaaa aaaataaaat
     5101 aaaggatatg ttatgaagcc aatttgtatt attcctgcgc gatcagggtc aaaaggatta
     5161 ccagataaga atatgttatt cctagcaggt aaacccatga ttttccatac tatagatgct
     5221 gcaattgaat ctggaatgtt tgataagaaa gatatttttg ttagtacaga ttcagaattg
     5281 tacagagaga tttgtttaga acgcggtatt tcagtggtga tgagaaaacc ggaactttca
     5341 actgatcagg caacttcgta tgatatgtta aaagattttt tatctgacta tgaagataat
     5401 caggagtttg tgttacttca agtaacttct ccactaagaa aatcatggca tataaaggaa
     5461 gcaatggagt attattcttc acatgatgtt gacaatgttg taagtttttc tgaagttgag
     5521 aaacacccta gtctgtttac aacattatct gatgaaggct atgctataga tatggtggga
     5581 gcagataaag gttatcgtcg ccaagattta caacctttat actatccgaa cggcgctatt
     5641 tttatttcta ataaagaaac ttacttaagt gaaaaaagtt ttttcacctc taagacatat
     5701 gcttatcaaa tggcaaagga attttcatta gatgttgata cgagagatga ttttatccac
     5761 gtcatcggtc acctcttttt cgattatgcc attagggaaa aagagaataa agttttttat
     5821 aaagaaggct atagtcgttt gttcaataga gaagcttcaa agataatttt aggtgattca
     5881 aaaacgatat ctatctcact agaaagttac cataattatt cccaaggtgg tgtaacatta
     5941 gcaacgatgt tggaaaactt acctaacttt ttgacagcta atgtaacaga ggcttttgtt
     6001 tctatagggg taaatgatct cattacaggt tatagtgtgg aagaaatatt cagcaatttc
     6061 caaaaactct actcattatt agctgagaat aaaataaaga tgagattgac aactattgct
     6121 tacacccttt ttagagaaac tgtcaataat gcagatattg agaaaatcaa tcaatggcta
     6181 acagaatttt gttatcaaaa tcagattcca ttgttagata ttaatagatt tttatcaaag
     6241 gatggtaatc taaattatca tttaactagt gatggattac attttactca agaggctaat
     6301 gatttgttac aaagtcaata tcaattattt gtagacgaag ttaaaacctt ataatattta
     6361 ttttataaaa ttattcatct tatttttgcg atttcaatag tgatatggta tatttaaagt
     6421 aagctacgta tagttttacc agtcagaaat agtaaggatg gttgagtatg agagagactg
     6481 aaacacacga tcaccaagcg ctaattcaaa aattgttagt cagtattcat tatttaacct
     6541 tgtttcgtga tgaaattata ttagtagaaa aaaccccatc tttactgggg aaacattttt
     6601 ctattgctat tgtgcagaat gaactaggtg agatactttc taaaatagaa gctttgagta
     6661 agcaaaaaaa actgattcgt tctatttatt ggtatgatga atcgtcattt aaagtcatga
     6721 ataaggctct ggctattgta gaagaatgga ttaagggctt agataatctt ttagaattct
     6781 gtcaatcaca gacggttttt caagcaattt taggtgatga acgtgcacat gtatttggaa
     6841 tccttattga tgtttatact tctttaaaca ttataaatac ttctttgaaa gaagaaaatt
     6901 ctagaccagt gtccttatct gatttggctc taggtcttaa ggaagaagat taataaaaaa
     6961 acgttactgc tttgaatgaa taaagcagta acgtttttta taaaatattg ctatactagt
     7021 aatacagata tctaaaagta aggagttata aatgaaacat tctagttggc atgatttaat
     7081 aaaaagagaa ctacccaatc attactacaa taaaattaat acatttatgg atgcagttta
     7141 cgaaagcgga atcgtttatc ctccaagaga taaggtgttc aatgccatac agataacacc
     7201 actagaaaac gtaaaagtag ttattattgg ccaagatcct tatcatggac ctcaacaagc
     7261 tcaaggactc tcattttctg ttccagacaa tttaccagct cctccttcac ttcaaaatat
     7321 tctaaaagaa ttagcagaag atatcggatc acgttcacat catgacttaa catcatgggc
     7381 gcaacaagga gtgctgctat tgaatgcttg tttaacagtt ccagaacacc aagctaatgg
     7441 tcatgctggg cttatatggg aaccttttac tgatgctgtc atcaaagtgg ttaaccaaaa
     7501 ggaaacacca gtagtcttta ttctgtgggg tggctatgct cgaaagaaaa agtcgctaat
     7561 tgataatcct attcatcata ttattgagag tcctcatccg agtcccttat ctgcttatcg
     7621 tggctttttt ggtagcagac ctttttctag aacaaatcat ttcttagaag aagaaggaat
     7681 aaatgagatt gattggttaa attaaaaggg aaatctactc tcgaaacatt gttagtctta
     7741 taataaaaaa ccgctagtag cggtttttta ttatttattt ttttgtttac ttaggtttaa
     7801 gccaaacgga accaaatttt cttgcctttt tcgaatacgt ttgatattat cctgatgtcg
     7861 gataataatc gtgagggcca ttaagataac cacagtggta aatatccagt cgtagtccgt
     7921 taatataaaa cctactaatg gaaaaattaa gacactaagg atacctacga cagctactgt
     7981 aatactggat agcgaaatca tgctaaaaag ataaagcgtt aataaaaaga taactaataa
     8041 gtaaaggaaa aaagaaggtg caaatcctag caaaacccca gcgcttgtag caacggcttt
     8101 tccacctttg aattgtgcaa aaatagggaa agtgtgacct ataatagcaa aaaaaccgat
     8161 aaaaaatgga gatactgtcg ttatacctaa tatgatagga ataagtgttg ctagagtccc
     8221 ttttaaaatg tcaatagtca aagtgacaat gccagctttt acacctaaaa tacggaaggt
     8281 attcgtggtg ccagtattac cactaccgtg ttgacgaaga ttgacttggt aaaaatattt
     8341 accaatccaa agtccagttt ggatagagcc taacagatag gcaatgataa tcataattat
     8401 aatattcata ccct
//
LOCUS       DBJYSH010000088         8405 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.088, whole genome shotgun sequence.
ACCESSION   DBJYSH010000088 DBJYSH010000000
VERSION     DBJYSH010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 8405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8405
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.088"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            complement(<1..838)
                     /locus_tag="ACUBV3_001567"
     CDS             complement(<1..838)
                     /locus_tag="ACUBV3_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HHU3848060.1"
                     /translation="MDIFNQLPDSVLQWFAIFISIIIEALPFVLLGTILSGIIEVFIT
                     PDIVNKFLPKNKFLRVLFGTFVGFVFPSCECGIIPIINRFLEKKVPSYTAVPFLATAP
                     IINPIVLFATYSAFGNSIRFLILRFVGATIVAIALGVMLAFLVDDNILKEDAKPTHFH
                     DYSDKKWYQKIFLALAHAIDEFFDTGRYLVFGTLIASAMQIYLPTRVLTTIGHSPITA
                     ILVMMLLAFILSLCSEADAFIGASLLSTFGIAPVMAFLLIGPMIDIKNLMMMVNSFKT
                     RFI"
     gene            complement(844..972)
                     /locus_tag="ACUBV3_001568"
     CDS             complement(844..972)
                     /locus_tag="ACUBV3_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPJ_0845 family protein"
                     /protein_id="HHU3848061.1"
                     /translation="MAITHKRQDDLESMFASFAKVPKPKKVDSDSKPEQKDDSQKD"
     gene            1105..2150
                     /locus_tag="ACUBV3_001569"
                     /pseudo
     CDS             1105..2150
                     /locus_tag="ACUBV3_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571058.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
     gene            2254..4416
                     /locus_tag="ACUBV3_001570"
     CDS             2254..4416
                     /locus_tag="ACUBV3_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HHU3848062.1"
                     /translation="MENLNITQIAIDLGIKASQIEKVLELTDEGNTIPFIARYRKEMT
                     GNLDEVQIKSIIDLDKSMTALSDRKTTVLAKIEEQGKLTQELKKAIEEATKLADVEEL
                     YLPYKEKRRTKATIAREAGLFPLARLILQNKDNLEEEAQNYLTDGFETTTKALSGAVD
                     ILIEAFSEDNKLRSWTYNEIWNYSSITAVVKDESLDEKQVFKIYYDFSEKISKLHGYQ
                     VLALNRGEKMGVLKVNFEHNLEKMFRFFAVRFKETSQYIDDLIVQTVKKKIVPAMERR
                     IRTELSEGAEDGAISLFSENLRNLLLVSPLKGKMVLGFDPAFRTGAKLAVVDQTGKLM
                     TTQVIYPVPPANQAKIEQSKIELAKLIKEFNIEIIAIGNGTASRESEAFVAEVLQDFP
                     DVSYVIVNESGASVYSASELARHEFPDLTVEKRSAISIARRLQDPLAELVKIDPKSIG
                     VGQYQHDVSQKKLAENLDFVVETVVNQVGVNVNTASPALLAHVSGLNKTISENIVKYR
                     EENGQIKSRAEIKKVPRLGAKAFEQAAGFLRIPNAKNFLDNTGVHPESYEAVKKLLDQ
                     LTIKELDDLAKEKLQHLDLIATAESIGVGQETLKDIIEDLLKPGRDLRDDFEAPVLRH
                     DVLDVSDLKVGQELQGTVRNVVDFGAFVDIGVHEDGLIHQSRLIKRKRDKKTRKMPPL
                     PHPSKYLSVGDIVTVWVVEVDAERGRIGLSLIKPDGLD"
     gene            4379..4837
                     /locus_tag="ACUBV3_001571"
     CDS             4379..4837
                     /locus_tag="ACUBV3_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HHU3848063.1"
                     /translation="MDLVLSSQTDLTNYVKKVSIEDFGCQFLHTAHWNNRLRTTGGRF
                     FPNDRHLDFNPKIYREFGIDIFRKIVRHELCHYHLYIQGKGYRHRDKAFKELLEKVDG
                     LRYTPRVTAVKVNYHHYSCQSCGQVYRRRRRVNLRKFACGYCHGRLIESF"
     gene            4918..5181
                     /locus_tag="ACUBV3_001572"
     CDS             4918..5181
                     /locus_tag="ACUBV3_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HHU3848064.1"
                     /translation="MKSSFYKQRKGKLVCGVVAGLADKYNWDLALSRVLIALILYFTK
                     FGLLLYILLAVFLPYKEDIIETRRQGPRRRKDAEPVDDDGWFW"
     gene            5409..6344
                     /gene="hprK"
                     /locus_tag="ACUBV3_001573"
     CDS             5409..6344
                     /gene="hprK"
                     /locus_tag="ACUBV3_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HHU3848065.1"
                     /translation="MAVTVQMLVDRLKLNVIYGDEHLLSKRITTADISRPGLEMTGYF
                     DYYAPERLQLVGMKEWSYLMAMTGHNRYQVLREMFQKETPAIVVARDLEIPEEMYEAA
                     KDTGIAILQSKAPTSRLSGEVSWYLDSCLAERTSVHGVLMDIYGMGVLIQGDSGIGKS
                     ETGLELVKRGHRLVADDRVDVYAKDEETLWGEPAEILRHLLEIRGVGIIDIMSLYGAS
                     AVKDSSQVQLAIYLENFETGKVFDRLGNGNEEIELSGVKVPRIRIPVKTGRNVSVVIE
                     AAAMNHRAKQMGFDATQTFEDRLTHLISQNEVNDD"
     gene            6337..7110
                     /gene="lgt"
                     /locus_tag="ACUBV3_001574"
     CDS             6337..7110
                     /gene="lgt"
                     /locus_tag="ACUBV3_001574"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HHU3848066.1"
                     /translation="MINPVAIQLGPFSIRWYAICIVSGMLLAVYLAMKEAPRKNIKSD
                     DILDFILMAFPLSIVGARIYYVIFEWAYYSKHPVEIIAIWNGGIAIYGGLITGAILLV
                     IFSYRRLINPIDFLDIAAPGVMIAQAIGRWGNFINQEAYGRAVKNLNYLPNFIKNQMY
                     IDGAYRVPTFLYESLWNFLGFVIIMSIRHRPRTLKQGEVACFYLVWYGCGRFIIEGMR
                     TDSLYLAGLRVSQWLSVILVIIGIVMIIYHRREQHISYY"
     gene            7125..7523
                     /locus_tag="ACUBV3_001575"
     CDS             7125..7523
                     /locus_tag="ACUBV3_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HHU3848067.1"
                     /translation="MIEIAVLIIAIAFVVLVLGILFVLKKVSETIEETKQTIKVLTSD
                     VNVTLYQTNEILAKANVLVDDVNGKVSTIDPLFVAIADLSESVSDLNLQARHIGQKAS
                     SATSSVTKAGSALAIGKAASKIFRKKGDKK"
     gene            7520..7951
                     /locus_tag="ACUBV3_001576"
     CDS             7520..7951
                     /locus_tag="ACUBV3_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HHU3848068.1"
                     /translation="MSKLFKTLVISAASGAAAAYFLTTKKGKELRKNAEKFYGEYKEN
                     PEEYHQIAKDKASEYSNLAVDTFKDYKGKFESGELTTEDIVSAVKEKSGEVVDFANDF
                     VNQAKSKFSDEDTAKKEDKAPETKVEDIVIDYKENTEDKEK"
     gene            complement(7992..8267)
                     /locus_tag="ACUBV3_001577"
     CDS             complement(7992..8267)
                     /locus_tag="ACUBV3_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3270 domain-containing protein"
                     /protein_id="HHU3848069.1"
                     /translation="MSSPLKQEQTHESKTEQAQPKFQVFQEFDHSAKLRELVFFARIA
                     LFSITTVLISFLLLALSLTPVWSFLLAMLASLAVTSAVSSFIWSLRQ"
BASE COUNT         2798 a         1302 c         1711 g         2594 t
ORIGIN      
        1 caataaacct tgttttaaaa gaatttacca tcatcattaa attcttaata tcaatcatgg
       61 gtccaattaa aaggaaagcc atcactggcg cgattccaaa ggttgataat aaagaagcac
      121 ctataaaagc gtcagcttca ctacacaatg acaagatgaa agcaagcaac atcataacta
      181 agatggctgt gatagggctg tgtccgatag tagtaagaac tcgagttggt aaataaattt
      241 gcatagctga agcaattaaa gtgccaaata ctagatagcg tcctgtgtca aaaaattcat
      301 cgatggcatg agctaaggct agaaagattt tttgatacca cttcttatca gaataatcat
      361 gaaaatgagt aggtttagcg tcttctttta gaatattatc atctactaaa aaggcaagca
      421 tgactcctaa agcaatggct acaatggtag cacctacaaa gcgtaatatc aggaagcgta
      481 tagaatttcc aaatgctgag taggtcgcaa ataaaacaat aggattaata ataggagcag
      541 tagcaagaaa cggaactgcc gtgtatgaag gcactttctt ttcaaggaat cgattaataa
      601 taggaatgat accacattcg caagaaggaa agacaaagcc aacgaaggtt ccaaataaga
      661 ctctgagaaa tttatttttc ggtaagaact tattgactat atcaggtgtt ataaagactt
      721 caataattcc tgataaaata gttccaagca aaacaaaagg aagtgcctca ataataatgg
      781 aaatgaaaat ggcaaaccat tgaaggacac tatccggtaa ttgattaaag atatccatag
      841 aacttaatct ttctgagaat catctttctg ttcaggtttt gaatcagaat caactttttt
      901 aggttttggt actttagcaa aactagcgaa catactttct aagtcatctt gacgtttgtg
      961 cgtaatagcc attaatggca cctctttctt tatataataa cctattatac tatattagct
     1021 tatttttaac ataaaaaaac aaatcgattg gaaatgatac tatatagtga taaaatgtta
     1081 ttagtagatt gatgaggtaa gattatgatt gaattaggta ttacaacatt cggagagaca
     1141 acaatccttg aagaaacaaa ccaaagctat tcacatcctg agaggattcg ccaattagtt
     1201 gctgagattg aactagctga tcaagttggt ttagatgtat atggtattgg agagcaccat
     1261 cgtgaagatt ttgcggtctc tgcacccgaa attatcctag cagcagggga gcggttagaa
     1321 ctaataatat ccgtttatct agtgcagtaa cgattctctc ttccaatgat cctattcgcg
     1381 tctatcagca attttcaacg attgacgcac tttcaaatgg tagagcagaa attatggcag
     1441 ggcgtggttc ctttattgag tcttttccat tgtttggata cgatttagcg gattatgatg
     1501 atttatttaa tgaaaaaatg gatatgttgt tagcaattaa ctcagcgaca aatctcgatt
     1561 ggaaaggtca tttgacacaa acagttaatg agcgaccaat ttatccaaga gcattacaaa
     1621 gacagttatc aatatgggtg gcaacaggag gaaatgttga ttctacaatt cgtattgcag
     1681 aacaaggttt gccaattgtt tatgcaacta ttggtgggaa tcccaaagcc tttcgtcaat
     1741 tggtccatat ttataaagaa gttggtagtc gtaatggaca caaaccagaa caactaaaag
     1801 ttgctgctca ctcttgggga tggattgaag aggataatca aaccgctatt gaccgttatt
     1861 ttttccctac gaaacagacc gtcgataata ttgctaaggg acgccctcat tggtctgaaa
     1921 tgactaaaga gcagtattta cgttcagtag gtccagaagg tgctattttt gtaggaagtc
     1981 ctgaagtggt tgcacataaa attataggac ttgttgaagc gcttgagtta gaccgtttta
     2041 tgttacatct accagttggc tctatgcctc acaaagacgt cctaaatgcc atcaaacttt
     2101 atggtgagga agtagcacca attgttcgaa aatattttga aaagaattaa agcagacctg
     2161 gtctgttttt tattgttaga ttataaaaac gctatcttga aatcgctttt taaaactctt
     2221 cgtaagtcaa aaatatgcta taataaaagc gttatggaaa atttaaatat tacacaaatt
     2281 gctattgatt taggtattaa agcgtcacaa atcgaaaagg ttttagaatt aacagatgaa
     2341 ggaaatacta ttccttttat tgcccgttat cgtaaagaaa tgacaggtaa tttggatgaa
     2401 gttcaaatca aatcaattat tgaccttgat aagagtatga cagctctttc agatcgtaaa
     2461 actacggtct tagccaaaat tgaagaacaa ggaaagttga ctcaagaact caaaaaggct
     2521 attgaagaag caactaaact agcagatgtt gaggaactct acctacctta taaagaaaaa
     2581 cgtcgtacga aagctacgat tgcacgtgaa gcaggtcttt ttcctttagc acgtcttatt
     2641 ttacaaaata aggataacct tgaggaagaa gctcaaaact atcttacaga tggttttgag
     2701 acaactacga aggccctttc tggagcagta gatattctta tcgaagcttt ttctgaggat
     2761 aataaattac gttcatggac atataatgag atctggaact acagctcaat cactgcagtt
     2821 gttaaagatg agtcacttga tgaaaaacag gtcttcaaaa tctactatga cttttctgaa
     2881 aaaataagta aactccatgg ctatcaagtt ttagcattga atcgtggtga gaaaatgggt
     2941 gttcttaagg ttaattttga gcataacctt gaaaaaatgt ttcgcttttt tgctgttcga
     3001 tttaaagaaa ctagccaata tatagatgac cttattgtac agactgtcaa gaaaaaaatt
     3061 gttccagcta tggaacgccg tatccgtaca gaactaagtg aaggagcaga agatggtgca
     3121 atttcactgt tttcagagaa tcttcgtaac ttattgttag tctcaccgct taaggggaaa
     3181 atggtgttag gttttgaccc tgcctttcgt acaggagcaa aattagcagt agtagaccaa
     3241 actggaaaat taatgacaac acaagtgatt tatccagttc ctccagctaa tcaagcaaaa
     3301 atcgaacaat caaaaataga gttagcaaaa cttatcaaag aattcaatat agaaattatt
     3361 gctataggga atggaacagc gagtcgtgaa tcagaagcat ttgttgcaga agttttacaa
     3421 gattttccag acgtatccta tgtgattgtt aacgaaagtg gtgcctctgt ttattcagcc
     3481 tcagaattgg cacgtcatga atttccagac ttaacagttg aaaaacgttc ggctatctca
     3541 attgcaagac gtctgcaaga cccgttagca gaactagtca aaattgatcc aaaatctatt
     3601 ggtgtgggtc aatatcaaca tgatgttagt caaaaaaaat tagcagaaaa tcttgatttt
     3661 gttgttgaaa ctgtagttaa ccaagtaggt gttaatgtta atacagcaag tccagccttg
     3721 ttagctcacg tttcaggttt aaataaaaca atatctgaaa atattgttaa atatcgtgaa
     3781 gagaatgggc aaattaagtc acgtgctgaa attaagaaag tacccagatt aggagcaaaa
     3841 gcttttgagc aagctgctgg attcttacgt attcctaatg ctaaaaattt cttggataat
     3901 acaggcgttc accctgagtc ttatgaagct gttaaaaagt tgcttgacca actgactatc
     3961 aaagaattag atgatcttgc aaaagagaag ttacaacacc ttgacttaat tgcaacagca
     4021 gaaagtattg gggtaggtca agaaacatta aaagacatta tagaagatct tttgaaacca
     4081 ggtcgtgatt tgcgtgatga ttttgaagcg cctgttttga gacatgatgt tttagatgtc
     4141 agtgatttaa aagttggaca ggaattgcaa ggaactgttc gaaacgtcgt tgattttggt
     4201 gcttttgttg atattggtgt tcatgaagat ggtctaatcc accagtctcg ccttattaag
     4261 cgaaaacgcg ataaaaaaac tagaaaaatg cctcctttac cacatccaag caaatatctc
     4321 tctgttggtg atattgtaac tgtttgggtt gtagaggtag atgcggagcg tggcagaatt
     4381 ggacttagtc ttatcaagcc agacggactt gactaattat gttaagaagg tgtctataga
     4441 agattttgga tgtcagtttt tacatactgc acattggaat aatcgcttaa gaacaaccgg
     4501 aggacgcttc tttccaaacg atagacacct tgactttaat cccaaaattt atcgtgagtt
     4561 tggtattgat atttttcgta agattgtaag acatgagctt tgccattatc atctctatat
     4621 acaaggtaaa ggatatcgac atagggataa agcttttaaa gagttactag aaaaagtcga
     4681 tggcttacga tataccccta gagttacggc tgttaaagtg aattatcatc attactcttg
     4741 ccaatcttgt ggtcaagtat accgacgtag gagacgtgtt aatttacgca agtttgcttg
     4801 tggttattgt catggtcgct tgatagagag tttttaaaat cagtcgtaag gcctatttaa
     4861 ttttactgtc aaagtagtta taattaaaat atagagtaga tagaaagaag gataggtatg
     4921 aaatcaagct tctacaaaca acgaaaaggt aagttggtct gcggtgttgt cgccggtcta
     4981 gctgataagt ataactggga tttagctctt tcacgggtgt tgattgcact gattttgtat
     5041 tttaccaaat ttggtctttt gctatatatt ttattagctg ttttcttacc ctataaagaa
     5101 gatattattg aaacaagaag acaaggtccc agaagacgta aagatgctga acctgttgat
     5161 gatgacggtt ggttctggta attcatataa aggagatttg ctagacgaat ctcctttttt
     5221 cttagcttta tttagaccat tttttcaaaa tatcttcttt ttttcagaaa aacgcgttag
     5281 cgcttacttt tagtaaaaag tatgatataa tgtgggtgta aattaatgaa agtgtttagt
     5341 acctttgctc aactttaaat tgtagggtat agcaataggg atattcactt ttataagaag
     5401 gagtaagtat ggcagttact gttcaaatgt tagttgaccg cttaaagtta aatgtcatct
     5461 atggtgatga acatctatta tctaagcgga ttacaacagc tgacatttca cgtcctggtc
     5521 tagaaatgac agggtatttt gattattatg caccagagcg tttgcaactt gttggtatga
     5581 aagagtggtc atatttaatg gctatgacag gacataatcg ttatcaagtg ttacgtgaga
     5641 tgttccaaaa agaaacacca gctattgttg tagcacgtga tttagaaatt ccagaagaga
     5701 tgtatgaggc ggctaaagat accggtattg ctattcttca aagtaaggcc cctacaagtc
     5761 gtttatcagg tgaagtttct tggtatttag attcttgctt agctgagagg acaagtgttc
     5821 atggtgtctt gatggatatc tatggtatgg gtgttctgat tcaaggagat tccggtattg
     5881 gtaagagtga gacaggacta gagctagtta agcgaggaca tcgtttggta gcagatgacc
     5941 gtgttgatgt ttatgctaaa gacgaagaga cgctttgggg agagcctgct gagatattac
     6001 gtcatttact tgaaattaga ggtgtgggaa tcattgatat tatgagccta tatggagcta
     6061 gtgcggttaa agattcatcg caagtgcaat tagctatata ccttgaaaac tttgaaactg
     6121 gaaaagtttt tgatagactc ggaaatggca atgaagagat cgaattatct ggagttaagg
     6181 ttcctcggat acgtattcct gttaaaacag gccgaaacgt gtcagtagtt attgaagcag
     6241 cggctatgaa tcacagagct aaacaaatgg ggtttgatgc tacccagact tttgaggatc
     6301 gtttaacaca tttgattagt cagaacgagg tcaatgatga ttaatccagt agcaatacaa
     6361 ttaggcccct tttctattcg ctggtatgct atttgcatag tgtctggtat gttgttagca
     6421 gtttacttag caatgaaaga ggcccctcgt aagaacatta agtcggatga tattctagat
     6481 ttcattctca tggcttttcc actatccatt gttggagctc gtatttatta tgttatcttc
     6541 gaatgggctt attactctaa gcacccagta gagattatcg ccatttggaa cggtggtatt
     6601 gctatttacg gagggttaat taccggagcc attctccttg tgattttttc atatcgtcgc
     6661 ttgattaatc ccattgattt tctggatatt gctgctccag gagtgatgat tgcccaagcc
     6721 ataggtagat gggggaattt tattaatcaa gaagcatacg gtagggcagt aaaaaatctg
     6781 aattacttac caaactttat aaaaaatcaa atgtatattg atggagctta ccgagtacct
     6841 acatttttat atgagtctct ttggaacttc ctaggttttg tcatcatcat gtctattcgt
     6901 catcgaccgc gcactttaaa acaaggtgaa gtggcatgtt tttatttagt ttggtatgga
     6961 tgtggacggt tcattattga agggatgagg acagatagcc tttatttggc gggtttgcgc
     7021 gtgtcacaat ggttatcagt tattttagtt attatcggaa ttgtcatgat tatttatcat
     7081 cgtcgtgaac aacatatttc atattattaa acagaggagg cattatgata gaaattgctg
     7141 tattaattat tgccattgcg tttgttgttt tagttttagg aatacttttc gttctgaaaa
     7201 aagtatcaga aacgattgag gaaaccaagc aaactattaa agtattaaca agcgatgtta
     7261 acgtgactct ttaccaaact aatgaaattt tagccaaagc taatgtttta gttgatgatg
     7321 ttaatggtaa agtatctact attgatcctc tttttgtagc tattgctgac ttatcagaat
     7381 ctgtatctga tttaaaccta caggcaagac acattggaca aaaggcaagt agtgcaacct
     7441 caagtgttac taaggcaggg tcagctttag ctattggaaa agcggcttca aaaatttttc
     7501 gtaaaaaagg agacaaaaaa tgagtaaatt atttaaaact ttagttatca gtgctgcatc
     7561 aggtgcagca gctgcttatt ttttaacaac aaaaaaagga aaagagctaa ggaaaaatgc
     7621 agaaaaattc tatggagaat ataaagaaaa tccagaagaa tatcatcaaa tagctaaaga
     7681 taaagcaagt gaatattcaa atttagctgt tgatactttt aaagattata aaggtaaatt
     7741 tgaatcaggt gaattgacaa cagaggatat cgtctcagcc gttaaggaaa aaagcggaga
     7801 agtagttgac tttgctaatg attttgtcaa tcaagctaaa tcaaaattct cagacgagga
     7861 tactgctaaa aaagaagata aggctcctga aacaaaagta gaagatattg tcattgatta
     7921 taaagaaaac acagaagata aagaaaaata aaaaagaagc tactataagt agcttctttt
     7981 ttatgagatt tttattgtct gagtgaccaa atgaatgaag agactgcaga tgtaactgct
     8041 agacttgcca gcatcgctag taagaaactc cacacaggtg ttaaacttag tgctagtaat
     8101 aagaaagaga ttagcacagt ggttatactg aagagagcga tacgagcaaa aaagactagc
     8161 tcacgcaact tagcactgtg atcaaattct tgaaatactt gaaattttgg ttgagcttgc
     8221 tctgttttgc tttcgtgagt ttgctcttgc ttaagtggtg atgacatagc tttctccttt
     8281 gatactagca tatcatatca caaataatag agtttgtcac tagttttttg atactatgag
     8341 ttaaaataaa ggttttttct caaaaaaaca aaaacattgc ccatattagt agtagaatac
     8401 taaaa
//
LOCUS       DBJYSH010000089         8212 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.089, whole genome shotgun sequence.
ACCESSION   DBJYSH010000089 DBJYSH010000000
VERSION     DBJYSH010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 8212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8212
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.089"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            151..1272
                     /gene="mnmA"
                     /locus_tag="ACUBV3_001578"
     CDS             151..1272
                     /gene="mnmA"
                     /locus_tag="ACUBV3_001578"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HHU3848070.1"
                     /translation="MTDNSNIRVVVGMSGGVDSSVTALLLKEQGYDVIGVFMKNWDDT
                     DEFGVCTATEDYKDVAAVADQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMTLGADYVATGHYAQVTRDENGIVHMLRGADNNKDQTYFLSQLS
                     QEQLQKTLFPLGHLQKPEVRRIAEEAGLATAKKKDSTGICFIGEKNFKDFLGQYLPAQ
                     PGRMMTVDGRDMGEHAGLMYYTIGQRGGLGIGGQHGGDNKPWFVVGKDLSKNILYVGQ
                     GFYHDSLMSTSLTASEIHFTRDMPNEFKLECTAKFRYRQPDSKVTVYVKGNQARVVFD
                     DLQRAITPGQAVVFYNEQECLGGGMIDQAYRDDKICQYI"
     gene            1304..1915
                     /locus_tag="ACUBV3_001579"
     CDS             1304..1915
                     /locus_tag="ACUBV3_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000623753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HHU3848071.1"
                     /translation="MISKFILAFMAFFAIMNPISNLPAFMALVADDDQKISRRIAAKG
                     VLLAFVIIVIFVLSGHLLFNLFGITLAALKISGGILVGIIGYKMINGIHSPTNKNLEE
                     HKDDPMNVAVSPLAMPLLAGPGTIATAMGLSSGGLSGKLITILAFAILCVIMYVILIS
                     ANEITKFLGKNAMTIITKMMGLILMTIGIEMLITGIKIGFHLT"
     gene            2085..3986
                     /gene="mnmG"
                     /locus_tag="ACUBV3_001580"
     CDS             2085..3986
                     /gene="mnmG"
                     /locus_tag="ACUBV3_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HHU3848072.1"
                     /translation="MTHNFAENYDIIVVGAGHAGVEASLAASRMGCKTLLATINLEML
                     AFMPCNPSIGGSAKGIVVREIDALGGEMGKNIDKTYIQMKMLNTGKGPAVRALRAQAD
                     KALYAQTMKQTVEKQENLTLRQAMIDEILVEDGKVVGVRTATNQKFSAKSVVITTGTA
                     LRGEIILGDLKYSSGPNNSLASVTLADNLRDLGLEIGRFKTGTPPRVKASSINYEKTE
                     IQPGDEQPNHFSFMSRDEDYITDQVPCWLTYTNTLSHDIINQNLHRAPMFSGIVKGVG
                     PRYCPSIEDKIVRFADKERHQLFLEPEGRYTEEVYVQGLSTSLPEDVQVDLLRSIKGL
                     ENAEMMRTGYAIEYDIVLPHQLRATLETKVIAGLFTAGQTNGTSGYEEAAGQGLVAGI
                     NAALKVQGKPELILKRSDAYIGVMIDDLVTKGTLEPYRLLTSRAEYRLILRHDNADMR
                     LTEIGYEIGLVDEERYAIFKKRQMQFENELERLDSIKLKPVSETNKRIQELGFKPLTD
                     ALTAKEFMRRPQITYAVATDFVGCADEPLDSKVIELLETEIKYEGYIKKALDQVAKMK
                     RMEEKRIPPHIDWDDIDSIATEARQKFKKINPETLGQASRISGVNPADISILMVYLEG
                     RQKGRKNIN"
     gene            4053..6035
                     /locus_tag="ACUBV3_001581"
     CDS             4053..6035
                     /locus_tag="ACUBV3_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HHU3848073.1"
                     /translation="MKRFRFATVHLVLIGLILFGLLAICVRLFQSYTALLLAIFVALS
                     FVVALLYYQKITYELSEVEQIELLNDQTEVSLKSLLEQMPVGVIQFDLETNDIEWFNP
                     YAELIFTGDNGHFQSATVKDIITSRRNGTAGQSFEYGDNKYSAYLDTETGVFYFFDNF
                     MGNRRNYDSSMLRPVIGIISIDNYDDIMDTMLEADMSKINAFVTSFISDFTQSKNIFY
                     RRVNMDRYYIFTDYSVLNTLIKDKFDILNEFRKRAQENHLSLTLSMGISYGDGNHNQI
                     GQIALENLNTALVRGGDQIVVRENDSSKKALYFGGGAVSTIKRSRTRTRAMMTAISDR
                     LKVVDSVFIVGHRKLDMDALGASVGMQFFASNIVNASYVVYDPNDMNSDIERAIDYLQ
                     EDGETRLVSVERAFELITQNSLLVMVDHSKTALTLSKEFFNKFADVIVVDHHRRDEDF
                     PKNAVLSFIESGASSASELVTELIQFQQAKDKLSRSQASILMAGIMLDTRNFASNVTS
                     RTFDVASYLRGLGSNSMAIQKISATDFDEYRLINELILKGERIYDNIIVATGEEHKVY
                     SHVIASKAADTMLTMAGIEATFVITKNSSNIGISARSRNNINVQRIMEKLGGGGHFSF
                     AACQIQDKSVKQVRRMLLEIIDEDLRENSTVENRRD"
     gene            6041..6493
                     /gene="rplI"
                     /locus_tag="ACUBV3_001582"
     CDS             6041..6493
                     /gene="rplI"
                     /locus_tag="ACUBV3_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HHU3848074.1"
                     /translation="MKVIFLQDVKGKGKKGEVKEVPTGYAQNFLLKKNLAKEATTQAI
                     GELKGKQKSEEKAQAEILAQAKELKTQLESETTRVQFIEKVGPDGRTFGSITAKKIAE
                     ELQKQYGIKIDKRHIDLDHTIRAIGKVEVPVKLHKQVSSQIKLDIKEA"
     gene            6536..7891
                     /gene="dnaB"
                     /locus_tag="ACUBV3_001583"
     CDS             6536..7891
                     /gene="dnaB"
                     /locus_tag="ACUBV3_001583"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HHU3848075.1"
                     /translation="MVEVSELRVQPQDLLAEQAVLGSIFISPEKLIMVREFISPDDFY
                     KYSHKVIFRAMITLADRNDAIDAATVRNILDDQGDLQNIGGLGYIVELVNSVPTSANA
                     EFYAKIVSEKAMLRDIISKLTDTVNMAYEGNDSDEIIATAEKALVDINEHSNRSGFRK
                     ISDVLKVNYENLELRSQQTSDVTGLPTGFRDLDRITTGLHPDQLIILAARPAVGKTAF
                     VLNIAQNVGTKQNRPVAIFSLEMGAESLVDRMLAAEGMVDSHSLRTGQLTDQDWNNVT
                     IAQGALADAPIYIDDTPGIKITEIRARSRKLSQEVDDGLGLIVIDYLQLISGTRPENR
                     QQEVSEISRQLKILAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYRREGEEAEEIVEDNTVEVILEKNRAGARGTVKLMFQKEYNKFSSIAQFE
                     E"
     gene            7903..8190
                     /locus_tag="ACUBV3_001584"
     CDS             7903..8190
                     /locus_tag="ACUBV3_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HHU3848076.1"
                     /translation="MSDAFADVAKMKKIKEDIKSHEGQMVELTLENGRKREKNKIGRL
                     IEVYPSLFIVEYKDTAAVPGAIDNTYVESYTYSDILTEKTLIRYFDDESAE"
BASE COUNT         2709 a         1212 c         1724 g         2567 t
ORIGIN      
        1 tatcacaaca aaaacataca acttgacaac aaatagtgct ataatatata agcactttta
       61 tagtgtttct tagtcaatat gattgatcta agaacaactt ggcccttcaa gcctaagtta
      121 aaaataaaac tttttgatgg gagaattctt atgactgata attcaaacat tcgtgtcgtt
      181 gtaggcatga gtggaggtgt tgattcatca gtaactgccc tgcttttaaa agagcaaggt
      241 tatgatgtta ttggtgtttt tatgaaaaat tgggatgaca cagatgaatt tggtgtttgt
      301 acggctactg aagattataa agatgttgct gctgttgctg atcagatcgg cataccatac
      361 tactctgtca actttgaaaa agagtattgg gacagggttt tcgaatattt tttagcagaa
      421 tatcgagcag gtcgtacgcc aaatccagat gttatgtgta ataaggaaat taaatttaaa
      481 gcatttctag attatgcgat gacattaggt gctgactatg tagcaacagg tcattatgca
      541 caagtaacac gtgatgagaa cggaatagtt catatgctta gaggagcaga taataataag
      601 gatcaaactt attttctaag tcaattatct caggagcaat tgcaaaaaac gttatttcca
      661 cttggtcatt tacaaaaacc agaagttcgc cgtatagctg aagaagcagg acttgcgacc
      721 gctaagaaaa aagactctac tggtatttgt tttattggag aaaaaaattt caaggatttc
      781 ctgggtcaat atttaccggc tcaaccaggt cgtatgatga ctgttgatgg tcgtgacatg
      841 ggtgaacatg ctggtttaat gtattatact attggtcaaa gagggggcct tggtattggt
      901 gggcaacatg gtggagataa taaaccttgg tttgtagttg gaaaggattt aagtaaaaac
      961 attctttatg taggtcaagg tttctatcat gactcattga tgtcaactag cttgacagct
     1021 tctgaaatcc actttacacg tgatatgcct aatgaattta agctagaatg tacagctaag
     1081 ttcagatatc gtcaacctga cagtaaagtt acagtttacg taaaaggtaa tcaagcaagg
     1141 gttgtgtttg atgatttaca acgagcaatc acaccaggtc aagctgttgt tttttataat
     1201 gaacaagaat gtttaggcgg tgggatgatt gatcaggctt atcgtgatga taaaatctgt
     1261 caatacattt aattgacact taattattaa gtgaggtaat cgtatgattt caaaatttat
     1321 tttagctttc atggcttttt tcgccattat gaatcccatt tctaatttgc cagcttttat
     1381 ggctttagta gctgatgatg atcagaagat ttcacgtcgg attgcagcaa aaggagtatt
     1441 acttgctttt gtcattattg taatctttgt attaagtggc cacttacttt ttaacttatt
     1501 tgggattacc ctagccgccc taaaaatatc tggtggaata ttggtaggca ttataggtta
     1561 taaaatgatt aatggtatac attcaccaac caataaaaac ctagaggagc ataaggatga
     1621 tccaatgaat gtggcagttt caccattagc aatgcccctt ttggcaggtc cgggtactat
     1681 tgcaactgct atggggttat caagtggtgg tttatctggc aaactcatta ctatacttgc
     1741 ttttgccata ttatgtgtga taatgtatgt tattttaata agtgcaaacg aaattactaa
     1801 atttcttgga aaaaatgcaa tgactataat caccaaaatg atgggactta ttttgatgac
     1861 tatcgggata gagatgctaa taacaggcat aaaaattggt tttcatttaa catagcctat
     1921 cccctctggt tgacttttaa aagataaatg ctaaaataaa tttaacaata actatgatgg
     1981 tcaaagctca tggaatttgt aagtcttttt gacgtacaat tttcgagagt tttggctatt
     2041 tttgtgtttt aatgaattag actttgaaat tggaaaggaa taaaatgaca cataattttg
     2101 cagaaaacta tgatattatc gttgttggag cgggtcatgc aggtgtggaa gctagtctag
     2161 ctgctagtcg tatgggatgt aagacacttc tagccactat caacttagaa atgttagcat
     2221 ttatgccttg taatccttct ataggtggct ctgctaaagg tatcgttgtc agagagattg
     2281 acgctttagg tggtgaaatg ggtaaaaaca ttgataaaac ctatatacaa atgaaaatgc
     2341 ttaatacagg taaggggcct gctgtccgtg ccttaagagc acaagctgat aaagccctct
     2401 atgcacaaac aatgaagcaa actgtagaaa agcaagaaaa tcttacctta agacaagcta
     2461 tgattgacga aatacttgtt gaagatggaa aggttgtggg ggttcgtaca gcaactaatc
     2521 agaaattttc agcaaaatct gttgttataa caacaggtac tgctcttcga ggagagatta
     2581 tattaggtga tcttaagtac tcctcgggcc ctaataatag tttagcttct gtgacattgg
     2641 cagataattt aagagaccta ggattagaaa ttggtcgttt caaaactgga acacctcctc
     2701 gtgtaaaagc ctcctctatt aattatgaaa agacagagat acaacctggg gatgagcaac
     2761 caaatcattt ttcatttatg tctagggatg aagattatat tactgaccag gtaccgtgct
     2821 ggttgaccta tacgaatact ttgagtcatg acatcatcaa tcaaaatcta caccgtgctc
     2881 ctatgttttc tggaatcgtt aagggagttg gtcctagata ttgtccgtca attgaagata
     2941 aaattgtgcg ttttgctgat aaagagcgcc accaactctt cttagaacca gaaggtagat
     3001 acactgaaga agtttatgtt caagggctat caacgagtct tcctgaggat gttcaagttg
     3061 atttattacg ttctataaaa gggcttgaaa atgctgaaat gatgcgtaca ggctatgcta
     3121 ttgagtatga tattgtattg cctcatcaat taagagcaac actcgaaaca aaagtaattg
     3181 ctggtttgtt tactgcagga cagactaatg gtacttcagg ttatgaagaa gctgctggtc
     3241 aagggttggt tgctgggatc aacgctgctc ttaaagttca agggaagcca gaacttattt
     3301 taaaacgaag tgatgcttac attggtgtca tgattgatga tttagttacc aaagggactc
     3361 tagaaccata tcgtctgttg acaagtcgcg cagaatatcg tctgattctt cgtcatgata
     3421 atgcagatat gcgtttaaca gaaatcggtt atgagattgg cctagtagat gaagagcgtt
     3481 atgcgatatt taaaaagcgt caaatgcaat ttgaaaatga attagaacgt ttagactcta
     3541 taaagttaaa gccagtttct gagacaaata aacggattca agaattgggc tttaaacctt
     3601 tgacagatgc tttaactgcc aaagaattta tgcgccgccc acaaatcaca tatgcagtag
     3661 caacggattt tgttggttgt gctgatgagc cattagacag taaggttatt gaacttttgg
     3721 aaactgaaat taaatacgaa ggctatatta aaaaggcttt agatcaagtt gcaaaaatga
     3781 aacgaatgga agaaaaacgt attccccctc atattgactg ggacgacatt gattctatcg
     3841 caacagaagc tagacaaaaa ttcaaaaaaa ttaacccaga aacgctaggt caggctagtc
     3901 gtatttcagg agttaatcca gccgatatta gtattttaat ggtttacctt gaaggaagac
     3961 aaaaaggtcg taaaaatatc aattgataat agtgttcgcc cctgtttatc aggggcgttt
     4021 tgtgttataa ttaggtctta agaggttatt taatgaaaag atttcgtttt gcaaccgtcc
     4081 atttagtttt aataggcctg attttatttg gactattagc tatttgtgtt aggttatttc
     4141 aatcctatac ggctctatta ttggctattt ttgttgcttt atcttttgtt gttgccttat
     4201 tatattacca aaagataact tatgaactat ctgaagttga acaaattgaa ttacttaatg
     4261 atcaaacaga agtttctctt aagagtcttc ttgaacaaat gccagttggt gttatacaat
     4321 ttgatcttga aacaaatgat attgagtggt ttaaccctta tgcagaattg atttttactg
     4381 gggataatgg tcattttcag tctgctactg ttaaagatat cattactagt agacgtaatg
     4441 gaacggctgg gcaatcattt gaatacggtg ataacaaata ttcagcttat ttggatacag
     4501 agacgggtgt tttctatttc ttcgataatt tcatggggaa tagaagaaac tatgatagtt
     4561 caatgctacg tccagttatt ggtattattt ccattgataa ctacgatgat attatggata
     4621 ctatgttaga ggctgatatg tcaaagatta acgccttcgt gactagtttt atttctgatt
     4681 ttacacagtc taaaaatatt ttttatcgtc gtgtcaatat ggatcgatac tatattttta
     4741 ccgactatag cgttttaaat acattgataa aagataaatt tgatatttta aatgagtttc
     4801 gaaaacgcgc tcaggaaaac catttatcat taacattaag tatgggaatt tcttatggtg
     4861 atggtaacca taatcaaatt gggcaaattg ctttagaaaa tcttaataca gcactcgttc
     4921 gcggaggaga tcaaatagta gtacgtgaaa atgatagtag taaaaaagca ctttactttg
     4981 gtggtggtgc tgtttcaact attaaacgtt cacgaacacg taccagagct atgatgactg
     5041 ctatttctga ccgtttgaaa gtagttgata gtgtctttat tgtgggacac aggaaattgg
     5101 acatggatgc actaggtgca tctgtaggga tgcaattttt tgctagtaat attgttaatg
     5161 ccagttatgt agtttatgac ccaaatgaca tgaattctga tattgaaaga gcaattgatt
     5221 acttgcaaga agacggcgaa acacgtctcg taagtgttga gagagctttt gaattaatta
     5281 ctcaaaattc tttattagtc atggtagacc attctaaaac agctttaacc ttatctaagg
     5341 aattctttaa caagtttgca gatgttattg tggtagatca tcatagaaga gatgaagatt
     5401 ttccaaaaaa tgctgttctt tcatttatag aaagtggcgc aagtagtgcc agcgaattag
     5461 ttacagaatt gattcaattt caacaagcta aagataaatt aagccgcagt caagcaagta
     5521 ttttaatggc tggtatcatg cttgatacaa gaaattttgc atctaatgta acgagtagaa
     5581 cttttgatgt ggcaagctat ttacgcggtt taggtagtaa tagtatggct attcaaaaaa
     5641 tatcagctac agattttgat gaatatcgtt taattaatga attgatttta aaaggtgagc
     5701 gtatttatga taacatcatt gttgctaccg gagaagaaca taaagtttac tcgcatgtta
     5761 tagctagtaa agcagctgac accatgttga caatggcagg tattgaggct acttttgtta
     5821 ttactaaaaa cagttcaaat attggtattt cagcgcgcag tagaaataat atcaatgtcc
     5881 agcgtattat ggaaaaactt ggtggtggcg gacattttag ttttgctgct tgtcaaatac
     5941 aagacaagtc tgtaaagcaa gttagacgta tgttactgga aataattgac gaagatttaa
     6001 gagaaaatag tacagtcgaa aatagaaggg attaattgag atgaaagtta tttttttaca
     6061 agatgttaag gggaaaggaa aaaaaggtga ggttaaggaa gtacctactg gttatgctca
     6121 aaacttctta ttaaaaaaga atctagctaa agaagcaacg acacaagcta tcggtgaatt
     6181 aaaaggaaaa caaaagtccg aagaaaaagc acaagcagaa attttagcac aggcaaagga
     6241 gctgaaaaca caattagaat ccgaaactac ccgagtgcaa tttattgaaa aggtaggtcc
     6301 agatggtcgt acgtttggtt ctatcacagc caaaaagatt gctgaagagt tacaaaaaca
     6361 atacggcatt aaaattgaca aacgtcatat tgatttagat catactatta gagcgattgg
     6421 taaggttgaa gtaccagtaa aattacataa acaggtttct tcacagatta agcttgatat
     6481 caaagaagcg taaactgaga gatgataaag gttgacaagt caggaggaat gaagtttggt
     6541 agaagtatct gagctaagag tacaacctca agacctgttg gcagaacagg ccgttctagg
     6601 atctatcttt atctcaccag agaaactgat tatggtgaga gaatttataa gtccagatga
     6661 tttttataaa tattcacaca aagttatctt cagagcgatg attaccttag ctgatagaaa
     6721 tgatgcaatt gatgctgcta ctgttagaaa tattttagat gaccaaggtg atcttcaaaa
     6781 cattggtggg ttgggctata ttgttgaatt agttaatagt gttccaacaa gtgctaatgc
     6841 ggagttttat gccaaaatag tttctgagaa agccatgttg cgagatatca tttctaagtt
     6901 gacagatact gtcaacatgg cctatgaagg aaatgattct gatgaaatta ttgccaccgc
     6961 tgagaaggct ttagttgata ttaacgaaca cagtaatcgt agcgggttta gaaagatatc
     7021 tgatgtttta aaagttaatt acgaaaactt agaattacgt tcacagcaaa cctcagatgt
     7081 cacaggttta ccaactggat ttagagattt agacaggatt actacaggtt tacatccaga
     7141 tcaattaatc atcttagcgg cccgtcctgc agtaggtaaa acagcctttg ttttaaatat
     7201 tgctcaaaat gttggaacta aacaaaatag acctgtagct attttttcac ttgaaatggg
     7261 ggctgagagt ttagttgacc gtatgttggc agcagaggga atggttgatt ctcatagttt
     7321 acgaactggt cagttgacgg atcaagattg gaataatgta acgattgctc aaggagcttt
     7381 ggcggatgcg ccaatttata ttgatgatac gcccgggatt aaaattacag aaattagagc
     7441 acgctctagg aagttatctc aagaagttga tgatggttta ggtttaatcg tcattgacta
     7501 cctccaatta atttcaggaa ctagaccaga aaatcgtcag caagaagttt ctgaaatttc
     7561 aaggcagttg aagattttag ctaaagaatt aaaggttcct gttatcgcat taagccagct
     7621 ctctcgtggg gttgagcaaa ggcaggataa acgaccggtc ttatctgata ttcgtgaatc
     7681 tggatcgatt gaacaagatg ctgacattgt tgctttctta tatcgagatg attattatcg
     7741 tagagagggc gaagaagcag aggaaattgt agaagataat actgttgagg ttattttaga
     7801 aaagaaccgt gctggtgcgc gtgggacagt aaaattaatg tttcaaaagg aatataataa
     7861 attttctagt atagctcaat ttgaagaata ggagatgtta atatgagtga tgcatttgca
     7921 gatgttgcaa aaatgaaaaa aataaaagaa gatatcaagt cacatgaggg acagatggtc
     7981 gaacttactc tcgaaaatgg tcgtaagagg gaaaaaaata aaattgggcg cctaattgaa
     8041 gtttacccat ctttgtttat cgtagagtat aaggatactg cagctgtacc aggagcaatt
     8101 gataatactt atgtagagtc ttacacttat tcagatattt taactgagaa aactttaatt
     8161 cgttattttg atgacgagtc agcagaatag tatgaaaacc tcgactgagg tt
//
LOCUS       DBJYSH010000090         8127 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.090, whole genome shotgun sequence.
ACCESSION   DBJYSH010000090 DBJYSH010000000
VERSION     DBJYSH010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 8127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8127
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.090"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            39..1205
                     /locus_tag="ACUBV3_001585"
     CDS             39..1205
                     /locus_tag="ACUBV3_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HHU3848077.1"
                     /translation="MEKRLSLGALVLASTVLLAACGNVGGGASSTGTKIGKDIKVGYN
                     WELSGNVSSYGNSMKNGADLAVKEINAAGGVGGKKLKVLSQDNKSENAEAATVATNLV
                     TKGANVIIGPATSGAAASSTPKVNAAAVPMIAPAATQDNLVYGSDGKTLNQYFFRATF
                     VDNYQGKLLSQYATDNLKAKKVVLFYDNSSDYSKGVAKSFKESYSGKIVDSMTFSAGD
                     TDFQASLTKLKGKEYDAIVMPGYYTETGLIVKQARDLGISKPVLGPDGFDSPKFVQSA
                     TPVGASNVYYLTGFTTQGSTKAKAFHDHYVKAYGEEPSMFSALSYDAVYMAAKSAKGA
                     KTSIDLKKALAKLKDFKGVTGKMSIDKNHNVVKSAYVVKLDDGKTSSVNIISAK"
     gene            1311..2180
                     /locus_tag="ACUBV3_001586"
     CDS             1311..2180
                     /locus_tag="ACUBV3_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000941401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HHU3848078.1"
                     /translation="MLQQLINGLILGSIYALLALGYTMVYGIIKLINFAHGDIYMMGA
                     FMGYYLINHLHLNFFLALLIAMLGSAFLGAVIEYLAYRPLRKSTRIAALITAIGVSFL
                     LEYGMVYLVGADTRAFPQAIHTVKYNLGPITITNVQLIILGIALLLMLTLQFIVQKTK
                     MGKAMRALSVDSDAAQLMGINVNRTISFTFALGSALAGAGGVLIGLYYNSVQPLMGVT
                     PGLKAFVAAVLGGIGIIPGAAIGGFVIGILETLATALGVSDFRDGIVYAILILIFLIR
                     PAGILGKNIKEKV"
     gene            2183..3136
                     /locus_tag="ACUBV3_001587"
     CDS             2183..3136
                     /locus_tag="ACUBV3_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000662325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HHU3848079.1"
                     /translation="MKENMKSILSWLAIVVLGYLLISVLISMGIFNLYHIQIIETIGI
                     NVILAVGLNLIVGCSGQFSLGHAGFMAIGAYAVAIIGVKMPTYVGFLIAILVGTLVAG
                     GIALGVGIPTLRLKGDYLAIATLGVAEIIRILLVNGGDITNGAAGIMGIPPFTTWSLV
                     YGVAVVSLILAMNFLRSPLGRNTIAIREDEIAAESMGVDTTKVKVIVFVFGAILASIA
                     GSLQAGYVGTVMPKDFSFMMSVNVLIIVVLGGLGSMTGTVLAAILLGLLNMLLQDYAS
                     VRMIIYALALILIMIFRPSGLLGTKELTLSHLFRKTSKGGN"
     gene            3137..3901
                     /locus_tag="ACUBV3_001588"
     CDS             3137..3901
                     /locus_tag="ACUBV3_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HHU3848080.1"
                     /translation="MALLEVKNLSKHFGGLTAVGDVSMKLHKGELIGLIGPNGAGKTT
                     LFNLLTGVYLPSKGTISLDGKILNGRKPAKIASLGLGRTFQNIRLFKNMTVLDNVLVG
                     LSNHHLSHPIASFLRLPKYYHSEKALRKKALELLEIFGLKAYQDALAKNLPYGKQRRL
                     EIVRALATEPKILFLDEPAAGMNPQETAELTQLISQIKSDFDITIMLIEHDMNLVMQV
                     TERIYVLEYGRLIAHGTPEEIKNNKRVIEAYLGGEL"
     gene            3901..4611
                     /locus_tag="ACUBV3_001589"
     CDS             3901..4611
                     /locus_tag="ACUBV3_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HHU3848081.1"
                     /translation="MTMLKVENLSIHYGVIQAVNDVSFEVNQGEVVTLIGANGTGKTS
                     ILRTISGLVRPSQGSISFMGKPIHKLAARKIVGNGLAQVPEGRHVFSSLSVMENLEMG
                     AFLQKDREQNQKMLKKVFDRFPRLEERKNQDAATLSGGEQQMLAMGRALMSRPKLLLL
                     DEPSMGLAPIFIQEIFNIIEDIKKQGTTVLLVEQNANKALTIADKAYVLETGKVVLSG
                     TGKELLVSDQVRKAYLGG"
     gene            4630..5289
                     /locus_tag="ACUBV3_001590"
     CDS             4630..5289
                     /locus_tag="ACUBV3_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001934124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HHU3848082.1"
                     /translation="MPVKDFMTKKLVYVSPDTTVAEAADLLREHHLRRLPVVENDQLL
                     GLVTEGTMAEAQPSKATSLSIYEMNYLLNKTKIRDIMIKDIVTVSQYASLEDAIYLMM
                     SRKIGVLPVVDNGQLYGIITDRDVFKAFLEIAGYGQEGYRLVILADEGIGVLSKVLNR
                     LSSANLSVKRLVIIERKAGKKAVEIQLEGYADKDVLKQELVFDDVIVETLEKTVKKPL
                     S"
     gene            5378..6013
                     /gene="tmk"
                     /locus_tag="ACUBV3_001591"
     CDS             5378..6013
                     /gene="tmk"
                     /locus_tag="ACUBV3_001591"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000715594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - dTMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HHU3848083.1"
                     /translation="MKKGLMISFEGPDGAGKTTVLEAVLPLLREKLSQDILTTREPGG
                     VTISEEIRHIILDVKHTQMDKKTELLLYMAARRQHLVEKVLPALEEGKIVLMDRFIDS
                     SVAYQGSGRGLDKSHIKWLNDYATDSHKPDLTLYFDVPSEVGLERIQKSVQREVNRLD
                     LEQLDMHQRVRQGYLELADSEPNRIVTIDASQQLDEVIAETFSIILDRINQ"
     gene            6033..6896
                     /locus_tag="ACUBV3_001592"
     CDS             6033..6896
                     /locus_tag="ACUBV3_001592"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HHU3848084.1"
                     /translation="MDLKRTQPKLLEKFNTILQSDRMSHAYLFSGNFASLDMALYLAQ
                     SQFCEKRQSGLPCQKCRACRLIANGEFSDVKIIEPQGQLIKTETIKELTKDFSRSGFE
                     GKSQVFIIKDCEKMHVNAANSLLKFIEEPQSSSYVILLTNDENNVLPTIKSRTQIFRF
                     LKQLDMLVHQAEQAGLLKSQASLLAQVADDPKHLEILLANKKLLDYLNLSQQFVTTLA
                     KDRQTAYLEVSRLTSQVVDKNDQAFVFQWLTIMLAKEGQLYDLENTYRAQQMWKSNVS
                     FQNSLEYMVLS"
     gene            6927..7253
                     /gene="yabA"
                     /locus_tag="ACUBV3_001593"
     CDS             6927..7253
                     /gene="yabA"
                     /locus_tag="ACUBV3_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HHU3848085.1"
                     /translation="MDKKDLFDAFDDFSQNLLVGLSEIETMKKQIQKLLEENTVLRIE
                     NGKLRERLSVIEAETETAVKNSKQGRELLEGIYNDGFHICNTFYGQRRENDEECAFCI
                     ELLYRD"
     gene            7253..8122
                     /gene="rsmI"
                     /locus_tag="ACUBV3_001594"
     CDS             7253..8122
                     /gene="rsmI"
                     /locus_tag="ACUBV3_001594"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000425856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HHU3848086.1"
                     /translation="MEMQVQKSFKSNTHYGTLYLVPTPIGNLDDMTFRAIRILREVDF
                     ICAEDTRNTGLLLKHFDITTKQISFHEHNAYDKISGLIDLLKEGRSLAQVSDAGMPSI
                     SDPGHDLVKAAIEGGIPVVSIPGASAGITALIASGLAPQPHIFYGFLPRKKGQQITFF
                     ETKKDYPETQIFYESPFRVSDTLKHMKEIYGDRQVVLVRELTKLYEEYQRGTISQLLG
                     HIEKVPLKGECLIIVDGKRDTERVKDSSQQDPLVLVKEYIANGDKTNQAIKKVAKELN
                     LNRQELYASFHDL"
BASE COUNT         2617 a         1243 c         1716 g         2551 t
ORIGIN      
        1 tattatttta gttaccattt tcaaaaaagg agaaaaatat ggaaaagaga cttagtttag
       61 gagctcttgt tttagctagt acagttcttt tagcagcatg tggaaatgtc ggaggaggag
      121 catcttcgac aggaacaaaa atagggaaag atattaaggt agggtataac tgggaattat
      181 caggaaacgt ttcttcgtat ggaaattcaa tgaagaatgg agctgactta gcagtaaaag
      241 aaattaatgc tgctggaggg gttggcggca agaagttaaa agtcctatca caggataata
      301 aatcagaaaa tgcagaggca gcaacggttg ccacaaactt ggttaccaaa ggagctaatg
      361 tcattatcgg accagcaaca tcgggtgcag ctgcatcttc aactccaaaa gtaaatgcag
      421 cagcagttcc aatgattgca cctgctgcga cacaagacaa tttagtctat ggttctgatg
      481 gaaaaacctt aaatcagtat ttcttccgag ctacttttgt cgataattat caaggaaagc
      541 tattgtctca gtatgctaca gacaacctta aagctaaaaa agttgttcta ttttatgata
      601 attcatcaga ttactcaaag ggggtagcaa aatcatttaa ggaaagttat agtggaaaaa
      661 ttgttgatag catgacattc tccgctggtg atactgattt ccaagcgtca ttgactaagt
      721 tgaaagggaa agaatatgat gctattgtga tgccaggtta ctataccgag acaggattaa
      781 tagttaagca agcgcgtgat ttaggtatct ctaaaccggt tcttgggcct gatggttttg
      841 atagtccgaa atttgtgcaa tcggcaacac ctgtgggagc ttcaaacgtt tattatttga
      901 caggtttcac tacacaagga tcaaccaaag ctaaagcttt ccatgatcat tacgttaagg
      961 catatggtga agaaccatcc atgttctcgg ctttatcata cgatgccgtg tatatggcag
     1021 ctaaatctgc taaaggagct aaaacttcaa ttgatcttaa aaaagcttta gctaagctga
     1081 aagattttaa aggtgtgaca gggaaaatgt ctattgataa aaatcataat gttgttaaat
     1141 cagcttacgt tgtcaaatta gacgatggaa aaacaagtag cgttaacatt atttcagcaa
     1201 aataagtatt ctctagaaaa atgggagttt aattttcagc tccccttttt taaaaagtaa
     1261 tttttcagaa taattcgaca ataatatata atagaaagta tggttataat atgcttcaac
     1321 agctcattaa tggacttatc ttaggatcca tatatgcttt acttgcctta ggatatacta
     1381 tggtatatgg cattattaag ctgattaatt ttgctcatgg ggacatctat atgatgggcg
     1441 ctttcatggg ctattatttg attaatcatc ttcatcttaa ttttttccta gctttattaa
     1501 ttgcaatgct ggggtctgct ttcctaggtg ctgtaattga atatttagct tatcgtccac
     1561 tgagaaaatc aactagaatt gccgcgttaa ttacagctat tggtgtttct tttcttttgg
     1621 agtacgggat ggtatacctt gttggagcgg atactagggc atttccgcaa gccattcata
     1681 ctgttaaata taatttagga ccgataacta taaccaatgt tcagctcatc attttaggta
     1741 ttgcgctatt actaatgctt actttacagt tcatcgttca aaaaactaaa atggggaaag
     1801 ctatgagagc tttatcagtg gatagcgatg ccgcacaatt gatgggaatt aatgttaacc
     1861 gtaccattag ttttacattt gcgctggggt ctgctttagc tggagcaggt ggagtactga
     1921 ttgggcttta ttataattct gttcaacctc ttatgggggt aactccaggg ttgaaagcat
     1981 ttgttgcagc tgtcttaggt ggtattggta tcattccggg agcggctatc ggtggttttg
     2041 ttattggcat tttagaaact ttagcgactg cacttggtgt gtcagatttt cgagatggta
     2101 tcgtttatgc tattttaatt ctcattttct tgatccgtcc agcaggaatt cttggaaaaa
     2161 atattaagga gaaggtctag gcatgaaaga aaatatgaaa tcaattttat cttggttagc
     2221 tatagtagtg ttagggtatc tcttgattag cgtgctaata tctatgggta tatttaacct
     2281 ctatcatatt caaattattg aaacaattgg gattaatgtt attttagcag ttggtttgaa
     2341 cctaattgta ggttgttcgg gacagttttc actaggacat gcaggtttca tggctattgg
     2401 cgcttatgca gttgctatta tcggcgttaa aatgccaact tatgtaggtt ttttgatagc
     2461 tatacttgtt ggaactcttg ttgcaggagg tattgcctta ggtgtaggaa ttcctacttt
     2521 gagactaaaa ggtgattatt tagctatagc tactttaggt gttgctgaga ttatccgtat
     2581 tttgttggtt aatggaggtg atattacgaa tggtgctgcg ggtatcatgg ggattccgcc
     2641 ttttacaacc tggtctttag tttatggggt ggcagttgtg agtcttatcc ttgcaatgaa
     2701 ttttttgcgt agtcccttag ggcgtaatac aattgctata cgagaagatg aaattgcagc
     2761 agagtctatg ggtgtggata caacaaaagt gaaagtaatt gtttttgttt ttggagctat
     2821 tttagcttct atcgctggta gtttacaggc tggctacgtt ggaactgtta tgccaaaaga
     2881 cttttcattt atgatgtctg tcaatgtttt aattattgtt gttttaggtg gtttaggttc
     2941 aatgacaggg acagttttag cagcaatttt attaggatta ttgaatatgt tactacaaga
     3001 ctacgcttct gttcgaatga ttatctatgc tttagctctg attttaatta tgatttttag
     3061 acctagcggt ttattaggaa cgaaggagtt aactctatct catctatttc gtaagacgag
     3121 taagggaggc aattagatgg cacttcttga agtaaaaaat ttaagtaaac atttcggtgg
     3181 cctaactgct gtaggtgatg tttcgatgaa attacataaa ggtgaattaa ttggcttaat
     3241 cggccccaac ggtgcgggta agacaacgct ttttaatctc ttaacaggag tataccttcc
     3301 tagtaaaggg acaatatcac ttgatggtaa gatattaaat gggaggaaac cagctaaaat
     3361 agcttcttta ggtttaggta gaacttttca aaatatccga ctttttaaaa atatgactgt
     3421 tttagacaat gtactagtag gactcagtaa tcatcactta tcacatccta ttgctagttt
     3481 tttacgatta cccaaatact atcatagtga aaaagcctta aggaaaaagg cactagagct
     3541 attagagatt tttggtttga aagcatatca agatgctctt gctaagaacc tcccttatgg
     3601 aaaacaacgg cgtttagaaa ttgttcgtgc tttagcaaca gaaccaaaaa tattgttttt
     3661 ggatgagccc gcggcaggaa tgaatcctca agaaactgca gaattgacgc agttaattag
     3721 tcaaataaag agtgattttg atattactat tatgctaatt gaacatgata tgaacttggt
     3781 tatgcaagta acagagagaa tttatgtatt ggaatatggt cgtctgattg ctcacgggac
     3841 acctgaagaa attaaaaata ataaacgtgt tatagaagca tacttggggg gtgagttata
     3901 atgaccatgt tgaaagtaga aaatctatct attcattatg gtgttattca agctgtgaat
     3961 gatgtctcgt ttgaagttaa ccaaggtgag gttgtaacct taattggagc caatggtaca
     4021 ggaaagacat caattttgag gacaatttct ggtttagtaa gaccaagtca gggttctatt
     4081 tcttttatgg ggaaacctat acataagtta gcagctagaa aaattgttgg caatggttta
     4141 gctcaggttc cagaagggcg ccatgttttc tcaagtttgt ctgttatgga aaatttagaa
     4201 atgggagcct ttcttcaaaa ggatcgtgaa caaaatcaaa aaatgttaaa aaaagtgttt
     4261 gatcgttttc ctcgcttaga agaacgtaaa aaccaagatg cagcaacctt atcaggtggt
     4321 gagcagcaaa tgctcgctat ggggcgtgca ttgatgagtc gtcctaaatt attactttta
     4381 gatgaaccgt caatgggcct agctccaatt tttattcaag aaatttttaa tatcattgaa
     4441 gacatcaaaa aacaagggac tacagttctt ttagttgaac aaaatgcaaa taaagctctg
     4501 acgattgctg ataaagcata tgttctagag actgggaagg ttgttttatc agggacaggt
     4561 aaggaattac tagtttcaga tcaagttcgt aaagcttact taggtggata atatttagag
     4621 gagtcaggta tgccagtgaa agattttatg acaaaaaaat tagtttatgt ctctccagac
     4681 actacagttg cagaagctgc tgatttattg agagaacacc acttaaggcg tttgccagtt
     4741 gttgaaaatg atcagctatt ggggttagtg acagaaggga caatggcaga agcacaacct
     4801 tctaaagcaa ccagtctttc tatatatgag atgaactatc ttcttaataa gacaaaaatt
     4861 cgagatatta tgattaaaga tattgtgact gtttcacagt atgctagcct tgaggacgct
     4921 atttatttga tgatgtctcg aaagatcggt gttttaccag tagttgataa tggacaactt
     4981 tatgggatta tcacggatag ggatgttttt aaagccttcc ttgaaattgc ggggtatggt
     5041 caagaaggtt atcgcttggt tatattagct gatgagggga taggggtact atcaaaagtc
     5101 ctaaatcgtc tttctagtgc aaatttaagt gttaaacgtt tagtcattat tgaacgaaaa
     5161 gcaggtaaga aagcggtcga gatacaacta gaaggatacg ctgacaagga tgttttaaaa
     5221 caagaactag tatttgatga tgttattgtg gaaactttag aaaagaccgt gaaaaaacca
     5281 ctttcttaaa aatatcagac atgcacaatc aagttgtggg tgtctttttt tgataaattt
     5341 tgatagaata gtagtaatta taaataaagg atgtaccatg aaaaaaggac taatgatttc
     5401 ttttgagggc ccagatggtg cgggtaaaac aaccgtttta gaagctgttt tacccttgct
     5461 cagagaaaaa ttatcacaag atattctcac caccagagag cctggtggcg taactatttc
     5521 agaagaaatt cgtcatatta ttttagatgt taaacacacg caaatggata aaaaaacaga
     5581 gctattgctt tacatggctg ctcgacgcca acatctagtg gaaaaggtac tcccagctct
     5641 agaagaaggg aaaatagtcc taatggatcg ttttattgat agttctgttg cctatcaggg
     5701 atcaggaaga ggattagata agtcacatat aaaatggctt aatgactatg cgactgatag
     5761 tcataaacct gatttaactt tatattttga tgttccttct gaagtaggtt tagagcgtat
     5821 tcaaaaaagt gttcaaaggg aggtaaatcg tctagatctc gagcaattag atatgcatca
     5881 acgcgtccgt caaggttacc ttgaattggc ggattcggag ccaaaccgca ttgtgacgat
     5941 tgatgcctcc caacagctag atgaagtaat agctgagaca ttctctatta ttttagatcg
     6001 tatcaaccaa tgaatcagga agtacaaagg tcatggattt aaagcgaaca caacccaaat
     6061 tgctggaaaa gtttaatact attttacagt cagataggat gagtcatgcc tatctttttt
     6121 cgggaaattt tgcaagtcta gatatggcac tttatttagc acaaagtcag ttctgtgaaa
     6181 agcgccagag tggtttacca tgccaaaagt gtagagcttg tcgtttaatc gctaatggag
     6241 aattttcaga tgttaaaatc atagaaccac aggggcaact tattaagaca gagactataa
     6301 aagagctgac taaagatttt tcaagatcag gtttcgaagg gaaatcacag gtctttatca
     6361 tcaaagattg cgaaaaaatg catgtcaatg cagccaacag ccttttgaaa tttattgaag
     6421 aacctcaaag ttcatcttat gtcatcttat tgactaatga tgaaaacaat gttttgccaa
     6481 caattaagag tagaacgcaa atttttcgtt ttctaaaaca attggacatg ttggttcatc
     6541 aagctgaaca agcaggattg ctaaaatcac aagctagctt attagctcaa gtagcagacg
     6601 atcctaaaca tttagaaata ttgttggcta acaaaaagtt attagattac ttgaatttaa
     6661 gtcaacagtt tgtgacgacc ttggcaaaag ataggcaaac tgcttattta gaagtatctc
     6721 gcttgacatc gcaagttgta gataaaaatg atcaggcatt tgtttttcaa tggctgacaa
     6781 taatgctggc taaagaaggc caactttatg atttagaaaa tacttataga gctcagcaaa
     6841 tgtggaagag caatgttagt tttcaaaata gtttagagta tatggtgctt tcttagacaa
     6901 ataatacgcg cagagaggaa gagtgaatgg ataaaaaaga cttgtttgat gcctttgatg
     6961 atttttcaca aaatttatta gttgggttgt ctgaaattga gactatgaaa aaacaaatcc
     7021 aaaaattatt ggaggagaat actgttctac gtattgaaaa tggtaaactt cgtgagcgtc
     7081 tgagcgttat tgaagcagaa acagaaactg cagttaaaaa ttctaaacaa ggaagagaat
     7141 tactcgaagg catttacaat gatggctttc acatttgtaa tactttctat ggtcaacgtc
     7201 gtgaaaatga cgaagaatgt gctttttgta ttgaattatt atatagagat tgatggaaat
     7261 gcaagttcaa aaaagtttta aatcaaatac acattacggg acactctatc tagtcccaac
     7321 tccaattggt aatctagatg atatgacttt tcgtgccatt aggattttaa gagaagttga
     7381 ttttatttgt gcagaggata cacgaaatac gggactttta ctcaagcact ttgatattac
     7441 tactaaacaa attagttttc acgaacacaa tgcttacgat aaaatctctg ggttaattga
     7501 tttgttaaaa gaagggagat ctttagccca agtatctgat gcaggaatgc cctctatttc
     7561 tgacccagga catgaccttg tcaaggctgc tattgaaggg gggatcccgg tcgtatctat
     7621 accaggagct agcgctggta ttactgctct catcgcttca ggtttagctc cacaacctca
     7681 tattttttat ggcttcttac cgcgtaagaa aggtcaacaa ataacttttt ttgaaacaaa
     7741 gaaagattac cctgaaacac aaatctttta tgagtcaccg tttcgagtct ctgatacgct
     7801 aaaacacatg aaagagattt acggagatcg ccaagttgtt ttagtacgcg aattgacgaa
     7861 actctatgaa gagtatcaaa gaggaaccat tagtcaactt ttagggcata ttgaaaaagt
     7921 ccctctcaaa ggtgaatgct taattattgt tgatggtaag agagataccg agcgagtgaa
     7981 agacagtagc caacaagatc cactagtatt agtaaaagaa tatatcgcta atggcgataa
     8041 aactaatcaa gcgataaaaa aagtagcaaa agaacttaat ctcaatagac aagaactcta
     8101 tgctagtttc catgatttat aagtaat
//
LOCUS       DBJYSH010000091         8078 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.091, whole genome shotgun sequence.
ACCESSION   DBJYSH010000091 DBJYSH010000000
VERSION     DBJYSH010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 8078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8078
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.091"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            9..1253
                     /locus_tag="ACUBV3_001595"
     CDS             9..1253
                     /locus_tag="ACUBV3_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HHU3848087.1"
                     /translation="MSKRQNLGISKKGAIISGLSVALIVAVGGFLWVQSQPNKSAVKT
                     NYKVFNVREGSVSSSTLLTGKAKANQEQYVYFDANKGNRATVTVKVGDKITAGQQLVQ
                     YDTTTAQAAYDTANRQLNKVARQINNLKTTGSLPAMESSDQSSSSSQGQGAQSTSGAT
                     NRLQQNYQSQANASYNQQLQDLNDAYADAQAEVNKAQKALNDTVITSDVSGTVVEVNS
                     DIDPASKTSQVLVHVATEGKLQVQGTMSEYDLANVKKDQSVKIKSKVYPDKEWEGKIS
                     YISNYPEAEANNNDSNNGSSAVNYKYKVDITSPLDALKQGFTVSVEVVNGDKHLIVPT
                     SSVTNKDNKHFVWVYNDSNRKISKVEVKIGKADAKTQEILSGLKAGQIVVTNPSKTFK
                     DGQKIDNIESIDLKSNKKSEVK"
     gene            1256..1966
                     /locus_tag="ACUBV3_001596"
     CDS             1256..1966
                     /locus_tag="ACUBV3_001596"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HHU3848088.1"
                     /translation="MTTNRKELIRLHQIVKSYQNGDQKLQVLKNIDLTVYEGEFLAIM
                     GPSGSGKSTLMNIIGLLDSPTSGDYSLNGKRVEELSQTKLAQVRNKEIGFVFQQFFLL
                     SKLTALQNVELPLIYAGVAPKKRKNLAKQFLDKVELRERMNHLPTELSGGQKQRVAIA
                     RALVNSPSIILADEPTGALDTKTGEQIMQFLTELNQEGKTIIMVTHEPEIADYATRKI
                     VIRDGEITADTTDSIRID"
     gene            1981..3228
                     /locus_tag="ACUBV3_001597"
     CDS             1981..3228
                     /locus_tag="ACUBV3_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000432854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HHU3848089.1"
                     /translation="MENWKFALSSILGHKMRAFLTMLGIIIGVASVVLIMALGKGMKD
                     SVTNEITKSQKNLQIYYKTKEDQKNEDNFGAQGAFMQSSDTNRKEPIIQESWLKKIAK
                     EVDGVSGYYVTNQTNAPVAYLEKKAKTVNITGINRTYLGIKKFKIKSGRQFQEEDYNQ
                     FSRVILLEEKLAQRLFQTNEAALNKVVTVKNKSYLVVGVYSDPEAGSGLYGSNSDGNA
                     ILTNTQLASEFGAKEAENIYFHLNDVSQSNRIGKEIGKRLTDISHAKDGYYDNFDMTS
                     IVKSINTQVGIMTGVIGAIAAISLLVGGIGVMNIMLVSVTERTREIGLRKALGATRRK
                     ILAQFLIESMVLTILGGLIGLLLAYGGTMLIANAQDKITPSVSLNVAIGSLIFSAFIG
                     IIFGLLPANKASKLNPIDALRYE"
     gene            3357..3629
                     /gene="rpsP"
                     /locus_tag="ACUBV3_001598"
     CDS             3357..3629
                     /gene="rpsP"
                     /locus_tag="ACUBV3_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HHU3848090.1"
                     /translation="MAVKIRLTRMGSKKKPFYRINVADSRAPRDGRFIETVGTYNPLV
                     AENQVTIKEERVLEWLSKGAQPSDTVRNLLSKAGVMTKFHDQKFSK"
     gene            3639..3881
                     /locus_tag="ACUBV3_001599"
     CDS             3639..3881
                     /locus_tag="ACUBV3_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HHU3848091.1"
                     /translation="MDTIENLIIAIVKPLISQPDQLTIKIQDGPEFLEYHLDLDTQDI
                     GRVIGKKGRTITAIRSIVYSVPTQGKKVRLIIDEKE"
     gene            4013..5524
                     /locus_tag="ACUBV3_001600"
     CDS             4013..5524
                     /locus_tag="ACUBV3_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator PRD
                     domain-containing protein"
                     /protein_id="HHU3848092.1"
                     /translation="MLFDFLEEKIKLKAYLMTALLDDGEINLDHFKTEYALSEKILQG
                     LISELQILYSDFYLVVKSRTMILFHYETLSKLETLHTIYRESNMLQFLAYFIAPQNVS
                     FSEFTAKKFISVASAYRIKKNCKLYLESVKLSIQKNKVTGPEYQIRLLIALLQYHFGF
                     IIYPFDATSEVMIRDFIEYSNFKGCDYDLKDLSEEYYYFYILVNLVWKRKEYKVEIPQ
                     TESFNQLKKSILYMELSKAAQKSIAIHLGIELNEGLLDYLYLVFCISDGPLFSNRDDI
                     NINEEFIFASYKISKSKAFKNFCGRLLGREFIDSQHFKDINAYFLRHFICYCYYFIPD
                     FYFLNTSRLSYSKDLYHLLDKGLADIFNLKGGNLTFSKHETVLLTMQLSNLIETFLAP
                     LSVYVISSSNIRLQTYQVMLNQYFTSKIAEFFFVNYQTTQIDEKLLKKADIIIAERRY
                     ISSFKNDSGVAIQDILEIGMNNKQYHELLLQAIIEKRDQKVFEYISKMKHKIL"
     gene            5632..5703
                     /locus_tag="ACUBV3_001601"
     tRNA            5632..5703
                     /locus_tag="ACUBV3_001601"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5664..5666,aa:Gln,seq:ttg)
     gene            5712..5783
                     /locus_tag="ACUBV3_001602"
     tRNA            5712..5783
                     /locus_tag="ACUBV3_001602"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5745..5747,aa:Glu,seq:ttc)
     gene            5846..6364
                     /gene="rimM"
                     /locus_tag="ACUBV3_001603"
     CDS             5846..6364
                     /gene="rimM"
                     /locus_tag="ACUBV3_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465641.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HHU3848093.1"
                     /translation="MEYFNVGKIVNTQGLQGEMRVLSVTDFVEERFKKGQVLALFDEK
                     NQFVMDIEIASHRKQKNFDIIKFKGMYHINDIEKYKGFTLKVAEDQLSDLKDGEFYYH
                     EIIGLDVYEGEELIGKIKEILQPGANDVWVVERHGKRDLLLPYIPPVVLEVDLSNQRV
                     QVELMEGLDDED"
     gene            6351..7106
                     /gene="trmD"
                     /locus_tag="ACUBV3_001604"
     CDS             6351..7106
                     /gene="trmD"
                     /locus_tag="ACUBV3_001604"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HHU3848094.1"
                     /translation="MMKIDILTLFPEMFAPLEHSIVGKAKERGLLEINYHNFRENAEK
                     SRHVDDEPYGGGQGMLLRAQPIFDTIDKIDAQKARVILLDPAGRTFDQDFAEELSKED
                     ELIFICGHYEGYDERIKSLVTDEVSLGDFVLTGGELAAMTMVDATVRLIPEVIGKETS
                     HQDDSFSSGLLEYPQYTRPYDYLGMTVPDVLMSGHHENIRKWRLEQSLRKTLERRPDL
                     LENYAMTDEERLILEKIKTEIERTDTVNEQNNL"
     gene            7087..>8078
                     /locus_tag="ACUBV3_001605"
     CDS             7087..>8078
                     /locus_tag="ACUBV3_001605"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HHU3848095.1"
                     /translation="MNKTIYDITIVGGGPVGLFAAFYAGLRGVSVKIIESLSELGGQP
                     AILYPEKKIYDIPGYPVITGRELIDKHIEQLERFKDSIEICLKEEVLSFEKVDDVFTI
                     QTDKDQHLSRAIVFACGNGAFAPRLLGLENEENYADNNLFYNVTKLEQFAGKHVVICG
                     GGDSAVDWANELDKIAASVAIVHRRDAFRAHEHSVDILKASGVRILTPYVPIGLNGDS
                     QRVSSLVVQKVKGDEVIELPLDNLIVSFGFSTSNKNLRYWNLDYKRSSINVSSLFETT
                     QEGVYAIGDAANYPGKVELIATGYGEAPVAINQAINYIYPDRDNRVVHSTSLIK"
BASE COUNT         2787 a         1195 c         1591 g         2505 t
ORIGIN      
        1 agatggacat gagtaaacga caaaatttag gaattagtaa aaaaggagca attatatcag
       61 ggctctcagt ggcactaatt gtagcagtag gtggcttttt atgggtacaa tctcaaccta
      121 ataagagtgc agtaaaaact aactacaaag tttttaatgt tagagaagga agtgtttcgt
      181 cctcaactct tttgacagga aaagctaagg ctaatcaaga acagtatgtg tattttgatg
      241 ctaataaagg taatcgagca actgttacag ttaaagtggg tgataaaatc acagctggtc
      301 agcagttagt tcaatatgat acaacaactg cacaagcagc ctacgacact gctaatcgtc
      361 aattaaataa agtagcgcgt cagattaata atctaaagac aacaggaagt cttccagcta
      421 tggaatcaag tgatcaatct tcatcatcat cacaaggaca aggggctcaa tcgactagtg
      481 gtgcgacgaa tcgtctacag caaaattatc aaagtcaagc taatgcttca tacaaccaac
      541 aacttcaaga tttgaatgat gcttatgcag atgcacaggc agaagtaaat aaagcacaaa
      601 aagcattgaa tgatactgtt attacaagtg acgtatcagg gacagttgtt gaagttaata
      661 gtgatattga tccagcttca aaaactagtc aagtacttgt ccatgtagca actgagggta
      721 aactccaagt acaaggaacg atgagtgagt atgatttggc taatgttaaa aaagaccagt
      781 ctgttaaaat aaaatctaag gtctatcctg acaaggaatg ggaaggtaaa atttcatata
      841 tctcaaatta tccagaagca gaagcaaaca acaatgactc taataacggc tctagtgctg
      901 taaattataa atataaagta gatattacta gccctctcga tgcattaaaa caaggtttta
      961 ctgtatcagt tgaagtagtt aatggagata agcaccttat tgttcctaca agttctgtga
     1021 caaacaaaga taataaacac tttgtttggg tatacaatga ttctaatcgt aaaatttcca
     1081 aagttgaagt caaaattggt aaagctgatg ctaagacaca agaaatttta tcaggtttga
     1141 aagcaggaca aatcgtggtt actaatccaa gcaaaacttt caaggatggg caaaaaattg
     1201 ataatattga atcaatagat cttaagtcta ataagaaatc agaggtgaaa taatcgtgac
     1261 aactaatcga aaagaactga ttagattgca tcagattgtt aaaagttatc aaaatgggga
     1321 tcaaaaactt caagtcttaa agaacattga tttgacggtt tatgaaggtg aatttcttgc
     1381 aatcatgggg ccatctggtt cggggaaatc cacactaatg aatattattg gcttgttgga
     1441 ttccccaact tcaggagatt atagtttaaa tggtaaacgt gtcgaagaac taagtcaaac
     1501 aaaattagct caagttcgaa ataaggaaat cggttttgtc tttcaacaat ttttcttgtt
     1561 atctaaacta acggctttgc aaaatgttga attaccctta atttatgcgg gtgtagctcc
     1621 taaaaagcgt aaaaatttag caaaacaatt tttagataaa gttgaattaa gagagcgtat
     1681 gaaccatctg ccaacagaat tatctggggg acagaagcaa cgtgtagcaa tcgcgcgtgc
     1741 tttggtgaat agcccatcta ttattctagc agatgagccg actggtgcct tggatacaaa
     1801 gacgggtgag caaattatgc agttcctaac ggaattaaat caagaaggta aaaccattat
     1861 catggtaact catgaaccag aaatcgccga ttatgcgaca cgtaaaattg ttattcgtga
     1921 tggcgaaatt acagcagata ctactgatag tattagaatt gattaagggg agattcgatt
     1981 atggaaaatt ggaaatttgc cctatcatcg attctagggc acaagatgcg tgcttttctg
     2041 accatgttag ggattatcat tggggttgca tcagttgtgt taattatggc cttaggtaag
     2101 ggcatgaaag attcggttac aaatgagatt acaaagtctc agaagaacct tcagatttat
     2161 tataaaacaa aagaagacca aaaaaatgag gataactttg gagctcaagg ggctttcatg
     2221 caaagtagtg ataccaaccg taaggaacca attattcaag aatcatggtt aaaaaagatt
     2281 gctaaggaag tagatggtgt tagtggctac tatgtaacta atcaaacaaa tgctccagta
     2341 gcttaccttg aaaaaaaggc aaagacggtt aatataacag gaattaaccg cacttacctt
     2401 ggtatcaaaa agttcaaaat taaaagtggt cgtcaattcc aagaggagga ttataaccaa
     2461 ttctcgagag ttattttact agaagaaaaa ttagctcaga gattatttca gacaaatgaa
     2521 gcagcgttaa ataaagtggt gactgtaaaa aataaatctt acttagtagt gggggtttat
     2581 tcggatccag aagctggttc aggtttatat ggaagtaatt ctgatgggaa tgctatttta
     2641 accaatacac agttagcttc ggagtttggt gctaaagaag cagaaaacat atattttcat
     2701 cttaatgatg tatcgcaatc caatcgcatt ggtaaagaaa taggcaaacg tttaacagac
     2761 attagtcatg ctaaggatgg gtactatgat aattttgaca tgacatctat agttaaatct
     2821 atcaataccc aagttggtat tatgacaggt gttattggag cgattgcagc aatttctctc
     2881 ctagttggtg gtatcggagt gatgaatatt atgttagtct ctgtaactga gcgcacgaga
     2941 gaaattggcc ttcgtaaagc ccttggggca actcgacgta aaattttagc tcaattttta
     3001 attgaatcaa tggtcttaac gattttgggt gggttaattg ggttattact agcctatgga
     3061 ggtacgatgt taattgctaa tgcgcaagat aagattactc cttctgtgtc acttaacgtg
     3121 gctataggca gtttaatttt ctcagcattt attggaatta tatttggttt attacctgct
     3181 aataaagcta gcaaattaaa cccaattgat gctttacgat atgaataaaa aaacatatgc
     3241 tctgtcaaga ttttttcttg acagagtttg tatttatgtt atacttagta aggtaaagtt
     3301 gttagttaag ctatcaacga ccaaatataa agaaaaagaa aagagactat ttaaaaatgg
     3361 cagtaaaaat ccgtttaact cgtatgggtt ctaagaagaa acctttctac cgtatcaatg
     3421 ttgcagattc acgtgcacca cgtgacggac gttttattga aactgttgga acatacaacc
     3481 cattagttgc agaaaaccaa gttacaatca aagaagaacg tgttcttgaa tggttatcaa
     3541 aaggtgcaca accttcagat acagttcgta accttctttc aaaagctgga gttatgacta
     3601 aattccacga tcaaaaattc tcaaaataat aaaagcctat ggataccatt gaaaatctca
     3661 ttattgctat tgtgaaacct ttgatttcac aaccagatca gttaaccata aaaattcagg
     3721 atggcccaga atttttagag taccatcttg atttagatac ccaagacatc ggtcgtgtta
     3781 tcggcaaaaa gggtcgtaca attacagcga taaggtcgat tgtctattcg gtaccaacac
     3841 aagggaaaaa agtaagactc atcattgatg agaaagaata atttattcta aaagatagga
     3901 acttcctgtc ttttttgtta tatctacaca aaaattgtga gttttttagt cttttagaat
     3961 ataatggatg tgaaagggaa ttttctgatt ttttattaag gaggataaga tattgctatt
     4021 tgattttctc gaagaaaaaa taaaactgaa agcatacctg atgaccgcct tacttgatga
     4081 tggagaaatt aaccttgatc actttaaaac agaatatgct ttgagtgaaa agattttaca
     4141 aggtcttatt tcggaactac aaatacttta ttctgatttc taccttgttg tcaaaagtag
     4201 aacaatgata ttgttccatt acgaaacact ttctaagcta gagactttgc ataccattta
     4261 tcgagagtct aatatgttac agtttttggc atattttatt gctcctcaaa atgtcagttt
     4321 ttctgaattc acggctaaga aattcatatc cgtcgcaagt gcctaccgaa taaagaaaaa
     4381 ctgtaaactt tatttggaga gtgttaagtt atcaatacaa aaaaataaag tgacaggtcc
     4441 agaatatcag attagactgc tgattgcttt gttacagtat cattttggtt ttattattta
     4501 tccttttgat gctacaagtg aggtaatgat aagggatttt attgaatact cgaattttaa
     4561 agggtgtgac tacgacctta aagacttatc tgaagaatat tactattttt atattttggt
     4621 gaatttagtt tggaaaagaa aagaatataa agtagaaatt cctcaaacag aatcctttaa
     4681 tcaactaaaa aaatcgattc tctacatgga gttatccaaa gcagctcaaa agagtattgc
     4741 tatacatctt ggtatagagt tgaatgaagg tttacttgat tatctatatt tagtgttttg
     4801 tattagtgat ggccctctat ttagcaatcg agatgatatc aatatcaacg aagaatttat
     4861 ttttgcttct tataagatat caaagtctaa agcctttaag aatttttgtg gtcgattatt
     4921 gggtagagaa tttattgact cgcagcattt taaagatatt aatgcctatt ttttgagaca
     4981 ttttatttgt tattgttatt attttattcc tgatttttat tttttaaata catcaagact
     5041 gagctattct aaggatttat atcacctatt agataaaggt ctggctgata tttttaacct
     5101 taagggaggg aatttaactt tttcaaaaca tgagacagtg ctactaacaa tgcaattatc
     5161 taacttaata gagactttct tagctcctct ttctgtatat gtaatttcat ctagtaatat
     5221 tagattacaa acctatcaag taatgttaaa ccaatatttt acatctaaga tagcggaatt
     5281 cttttttgta aactatcaaa caactcagat agatgaaaaa ttactaaaaa aagcagatat
     5341 cattattgct gagagacgct atatatcttc ttttaaaaat gactcaggtg tggctataca
     5401 agatatatta gaaattggga tgaataataa gcagtaccat gaactacttt tacaagctat
     5461 tatcgaaaaa agggaccaaa aagtttttga atatatttca aaaatgaaac ataaaatact
     5521 ttgatagact ctatatgagc tttattaaaa gggataaaat cgaaatttaa aatttaattc
     5581 gaaaagatat tgacagtaat ctagaatttc gatatactag attttgtctt ttggggtata
     5641 gccaagcggt aaggcaaggg actttgactc cctcatgcgt tggttcgaat ccagctaccc
     5701 cagtttcaca tggtccgttg gtcaaggggt taagacaccg ccttttcacg gcggtaacac
     5761 gggttcgaat cccgtacgga ctatcgtggc cagttgcatt ttagcggctg gtttttcttt
     5821 tattttcaaa aatatcggag ttactatgga atactttaac gttggaaaaa ttgtcaatac
     5881 acaaggatta caaggcgaaa tgcgtgtatt aagcgtgact gactttgtag aagaacgctt
     5941 taaaaaaggt caagtgttag ctttattcga tgaaaagaat cagtttgtta tggatattga
     6001 aattgctagt catcgtaagc agaagaattt tgacattata aaattcaaag gaatgtatca
     6061 tattaatgat attgaaaaat ataaaggttt cacactgaag gtggcagagg atcaactgag
     6121 cgaccttaaa gatggtgaat tttattatca tgaaattatt ggcttagatg tctatgaagg
     6181 ggaagagctg attggaaaaa tcaaagaaat tttacaacca ggagcaaatg acgtttgggt
     6241 tgtggaacgt catggaaaac gtgatctttt attaccctat atcccgccag tagtattaga
     6301 agtggacctt tctaatcagc gtgttcaagt tgaattgatg gaaggtttag atgatgaaga
     6361 ttgatatttt aactttattt cctgaaatgt ttgcccctct ggaacattct atagttggta
     6421 aggcaaaaga aagaggactt cttgaaatta attatcacaa tttcagagaa aatgctgaga
     6481 agtctcgaca tgttgatgat gagccttacg gtggggggca agggatgctt ttacgtgctc
     6541 aacctatttt tgatacaatt gataaaatag atgctcaaaa agcacgtgtt attttgctag
     6601 acccagcggg cagaacattt gatcaggatt ttgcagaaga actatccaaa gaagatgaat
     6661 taatttttat ttgtggtcac tatgaaggtt atgatgaacg tataaaatca ttagtaacag
     6721 acgaagtatc tttgggagat tttgttctta cgggaggaga attggcggct atgacaatgg
     6781 tcgatgctac cgttcgtctg attccagaag ttataggtaa agaaacaagt catcaggatg
     6841 attcattttc atcgggccta ttagaatatc ctcagtatac gcgcccatat gattatttag
     6901 gcatgacagt tcctgatgtt ctcatgagtg gtcatcatga aaatatccga aaatggcgtt
     6961 tagaacaaag tttgcgaaaa acactagaac gtcgaccaga cttgttagaa aattatgcaa
     7021 tgacggatga agagagactt atcttagaga aaattaaaac agaaatagaa agaacagata
     7081 cagtaaatga acaaaacaat ttatgatatc actattgtag gaggcggacc agtaggtctc
     7141 tttgctgctt tttatgctgg actacgcgga gtatccgtta aaattattga aagcctttcg
     7201 gaattaggag gccagccagc tatcctttat cctgagaaaa aaatctatga tattcctggt
     7261 tatccggtga ttactggacg tgaattaatt gataaacata ttgaacaatt agagagattt
     7321 aaagattcta ttgaaatctg tttaaaagaa gaagttttat catttgaaaa agtagatgat
     7381 gttttcacaa ttcaaacaga taaagatcaa catttgtcac gtgctattgt ttttgcttgt
     7441 ggcaacggtg cttttgctcc gcgtttacta ggtttggaaa atgaagaaaa ttatgcagat
     7501 aataatttat tctataacgt tacaaagtta gaacagtttg caggtaaaca tgttgttatt
     7561 tgtggtggag gcgactcagc tgttgattgg gccaatgaat tagataagat tgcagctagt
     7621 gtagcgattg ttcatcgtag agatgctttt agagcgcatg agcatagtgt ggatatacta
     7681 aaggcatcag gtgttagaat tttaacccct tatgtcccaa ttggtttaaa tggagatagt
     7741 cagagagtta gcagcttggt cgttcaaaaa gttaaaggtg atgaagtaat tgaattacct
     7801 ttagacaact tgattgttag ttttggcttt tctacttcta ataaaaattt acgttattgg
     7861 aatttggatt ataaacggtc tagtatcaat gtttcttcac tttttgagac gactcaagaa
     7921 ggcgtgtatg ctattggaga tgctgctaat tatccaggaa aggtagaatt gattgcaaca
     7981 ggatatgggg aagcaccagt cgctataaac caagctatca attatattta tcctgatcga
     8041 gataaccgtg tagtccattc aacatcattg ataaaata
//
LOCUS       DBJYSH010000092         7549 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.092, whole genome shotgun sequence.
ACCESSION   DBJYSH010000092 DBJYSH010000000
VERSION     DBJYSH010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 7549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7549
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.092"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            184..1923
                     /locus_tag="ACUBV3_001606"
     CDS             184..1923
                     /locus_tag="ACUBV3_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytS/YhcK type 5TM receptor domain-containing
                     protein"
                     /protein_id="HHU3848096.1"
                     /translation="MTLFLIMMERAGLIILLAYAFVHIPFIKQTLKQPELKKHQYILL
                     ILFSLFAIISNFTGVEIQSDLSIIPQTLNHIADQSSVANTRVLTIGVSGLIGGPIVGI
                     IVGLLSVFVRYLQGGLAPHIYVISSLLIGLCSGLSGNYLRKNYNKIRVLDAMVVGFGM
                     EILQMICILIFSVDFNQALRLVSFISMPMILSNTLGLGIFISIISSTQKLEEHAKAFQ
                     THQVLELANLTLPYLRKGLTTESCQPVAEIIHKHMDVSAVSLTSQSAILAYVGDGADH
                     HLPNTQILTKLAKRAIDTGKVSVATDKSEIECDHRNCPLSSAIVIPLHIHDVIVGTLK
                     LYFSDAQHMTYVDRQLAEGLGNIFSTQLALGQAEEATRLLQDAEMKSLQAQVNPHFLF
                     NALNTIYGLIRMDSEKARKLVQDFSKVIRANLQRAKQNLIPLHDELEQVNAYLALEEA
                     RFPNMVAFNLDNQTNSDDNLMIPPFTLQVLIENSYKHAFKHINKNNQLKVTIARNNDR
                     LHIIVQDNGIGIPKEKLITLGKKTQISKKGSGTAIENLVRRLNIIYDGQASLKFESND
                     SGTCAIVNIPIKN"
     gene            1935..2669
                     /gene="ltdR"
                     /locus_tag="ACUBV3_001607"
     CDS             1935..2669
                     /gene="ltdR"
                     /locus_tag="ACUBV3_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor LtdR"
                     /protein_id="HHU3848097.1"
                     /translation="MKVLVVDDEPVARNELIYLLNKYDSNLVIAEAHDMATALAILLR
                     ETFDVALLDIHLRDDSGLQLAEYINKMPKPPLLIFATAYDQYAIQAFEQDARDYLLKP
                     YEFDRLKQAMDRVKGALSTSTIIESVASGPLFKQQYPLTVEDRIYLVSADDILLIEAM
                     QGKLIIQTPDKNYEIDGSLQQWQDKLPSSQFVRVHRSYIVNINAIKTIEPWFNQTLQL
                     HLCNKITVPVSRANVKPLKQMLGIST"
     gene            2825..4309
                     /locus_tag="ACUBV3_001608"
     CDS             2825..4309
                     /locus_tag="ACUBV3_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HHU3848098.1"
                     /translation="MVTFLGGVALLIVGYFTYGRYIEKNFQIDENRQTPAEALRDGYD
                     FVPMPKWKNGMIELLNIAGTGPIFGPILGALYGPVAYIWIVLGCIFAGAVHDYMIGMI
                     SLRNNGAYLPELASRYLGKSMKHVINIFSMLLLILVATVFVVTPANLILSILPAGTLS
                     LPWIIGLIFVYYLISTVLPIDKALGKVYPVFCVILMVSTAAVGFRLLTGGFDMPNLTF
                     ETFKNMHPAGLGIFPALFFTISCGAISGFHATQAPMVSRTTVNEREGRFTFYGMMIAE
                     GVIAMIWAGASMSLFKGQNLYEMIAAGTPSAVVNQVMLMLLGSVIGTIAIIGVIVLPV
                     SSGLSAFRSLRTIVADYIHVKQDTLPKIFAVTIPLYVISFVLTHVDFNLLWRYFNWAN
                     QVTAVIGLLVATRYLILKRRNYWVTFVPAMFMLYAVVVYILSQPIGFNMGLGILTYSL
                     ALVLTGILVGLFWKSGQKQLKTVHPEAFLFNDHRPINYYSSLDS"
     gene            4511..5083
                     /locus_tag="ACUBV3_001609"
     CDS             4511..5083
                     /locus_tag="ACUBV3_001609"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001231800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HHU3848099.1"
                     /translation="MRKRSILPILAVLFVFMSIFSVYSIFHGDKAKEKASVIKQASQT
                     SQTSKKEVLQKKTYPNLNKYSNLEIHVSSTRQTMTITSSDKVIFKTIVSTGAKESPTP
                     KGTFVIEPERGDFFYNASSKEGAYYWVSFKEHGIYLFHSVPTDQQGNEIPEEAKQLGK
                     AASHGCVRMSRADAKWFYENIPQGTTVTIK"
     gene            5359..6210
                     /gene="ylqF"
                     /locus_tag="ACUBV3_001610"
     CDS             5359..6210
                     /gene="ylqF"
                     /locus_tag="ACUBV3_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HHU3848100.1"
                     /translation="MATIQWFPGHMSKARRQVQENIKHVDFVTILVDARLPLSSQNPM
                     LTKIVGDKPKLMILNKADLADPIRTKEWRDFYESQGLKTLAINSKEQSTVKKVTDIAK
                     ILMSDKIAKLRERGIQKETLRTMIIGIPNAGKSTLMNRLAGKKIAVVGNKPGVTKGQQ
                     WLKSNKELEILDTPGILWPKFEDELVGLKLALTGAIKDQLLPMDEVTIFGLNYFKTYY
                     PDRLKERFKSINLEDESPEIIMTLTQKLGYRDDYDRFYNLFVKEVRDGKLGRYTLDIV
                     GEHDGNN"
     gene            6197..6958
                     /locus_tag="ACUBV3_001611"
     CDS             6197..6958
                     /locus_tag="ACUBV3_001611"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HHU3848101.1"
                     /translation="MATIKEIKAILETIVDLKDKRWQEYQTDSRAGVQKAILQRKKNI
                     QSHLDEEARLEQMLVYEKKLYIEHINLIAGIDEVGRGPLAGPVVAAAVILPPNCKIKH
                     LNDSKKIPKKKHQEIYQNILDQALAVGIGIQDSQCIDDINIYEATKHAMIDAVSHLSV
                     VPEHLLIDAMVLDLPIPQTKIIKGDANSLSIAAASIVAKVTRDKIMSDYDSKYPGFAF
                     SKNAGYGTKEHLEGLQKYGITPIHRKSFEPIKSML"
     gene            6979..7527
                     /locus_tag="ACUBV3_001612"
     CDS             6979..7527
                     /locus_tag="ACUBV3_001612"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HHU3848102.1"
                     /translation="MTEKEKMLAGQYYRPSAPELRKDREVALKNMQAFNNEDDSSKRN
                     VILQKWFGATGKSIHMEQRFVCDYGCNIYVGENFYANFNQTFLDVCEIRIGDNCMFGP
                     NCQLLTPLHPLDPIERNSGLEYGAPIQIGNNVWLGGGVTILPGVVLGDNVVVGAGSVV
                     TKSFENNVVIAGNPAKIIKKLA"
BASE COUNT         2532 a         1275 c         1344 g         2398 t
ORIGIN      
        1 taatcaaaat tttttagtga aagtatctat catccttaag tagttgtttt cataatagtt
       61 gttgatggat atccccttaa ttgtcttgag aatacttgat agagagacat tttaaatgag
      121 taatgaaatg aatcatttta tacctatgtt ataacggact aattaatact ttggagagat
      181 ttaatgacac tctttttaat tatgatggag cgagcggggt taatcatatt attagcctat
      241 gcctttgtgc atattccctt tataaaacaa actttgaagc aacccgaatt aaaaaaacac
      301 cagtatattc tgcttatctt atttagctta tttgctatta tttctaattt tacaggggta
      361 gaaattcagt cagatttatc tatcattcca caaactctta atcatatagc agatcaatca
      421 tcagttgcca acacacgtgt tttaaccatt ggtgtatcag gtctaatcgg tggccccata
      481 gtcggtatta tagttggtct cttatctgta ttcgttaggt acttgcaagg aggattagcc
      541 cctcatattt atgtcatttc ttccttacta attggtttat gctctgggtt atcaggcaat
      601 tatcttcgta aaaattataa taaaattcgt gttttagatg caatggttgt tggtttcggg
      661 atggaaattc tacaaatgat ctgtatcctc atttttagtg ttgactttaa tcaggcgctt
      721 cgattagtat cttttatcag catgccaatg attttaagta atactttagg tttaggtata
      781 tttatatcca ttatttcatc tactcaaaaa ttagaagaac atgccaaagc atttcaaacc
      841 catcaagtac ttgaattagc aaatttaacc cttccttacc ttagaaaggg actgacaaca
      901 gaatcttgtc aaccagtagc tgaaattatt cataaacata tggatgtgag tgctgttagc
      961 ctgactagtc aatcagccat tttagcctat gtaggagatg gagctgacca ccatcttcct
     1021 aatactcaaa tattaacaaa gcttgctaaa agagcaatag atactggtaa agttagtgtc
     1081 gcaactgata aaagtgaaat cgaatgcgac cataggaatt gcccacttag ttcagcaatt
     1141 gttattccat tacacatcca cgatgttata gtgggtactt tgaaattata tttttcagac
     1201 gcacaacata tgacttacgt tgaccgtcaa ttagctgaag gattaggcaa tatcttttcc
     1261 actcagttag ctttgggaca agctgaggaa gcaacaaggt tactacagga tgcggaaatg
     1321 aagtctctcc aagcgcaggt taatccgcat tttcttttta atgcactcaa tactatttat
     1381 ggtttaattc gcatggatag cgaaaaagca agaaagctcg tccaagattt tagcaaggtt
     1441 attcgtgcta atcttcaacg ggcaaagcaa aatttaattc ctctccatga tgaattggaa
     1501 caggttaacg cctatttagc ccttgaagaa gcaagatttc ctaacatggt ggcctttaat
     1561 ttagacaacc aaacaaattc agatgacaac ttaatgattc ctccctttac attgcaggtc
     1621 ttgattgaaa actcttataa acacgctttt aagcatatca ataaaaataa ccagctaaag
     1681 gttactattg caagaaataa tgataggctt catatcattg tccaggacaa tggtattggg
     1741 attccaaaag aaaaattaat aacgcttgga aaaaaaactc aaatttcaaa aaaaggttct
     1801 ggtacagcta ttgagaatct cgttcgtcgc ttgaatatta tttatgatgg acaagcaagt
     1861 ttaaaatttg aatcaaatga ttcaggaact tgtgctattg ttaacatccc cattaaaaat
     1921 tagaaaggta ttgtatgaaa gttttagtag ttgatgatga accagttgca cgtaacgaat
     1981 taatttacct tcttaataag tatgattcta acctcgttat agcagaggcg catgatatgg
     2041 ctactgcatt agctatttta cttagagaaa cttttgatgt agcactgtta gatatccatc
     2101 tcagagatga ttctgggttg caattagcag agtatatcaa taaaatgccc aaaccaccat
     2161 tattgatatt cgcgactgct tatgatcaat atgctattca ggcttttgag caggatgcgc
     2221 gtgattattt gttaaaaccc tatgagtttg ataggttaaa gcaagctatg gatagagtaa
     2281 aaggagcgct aagtacatct acaattatag agagcgtagc ttccggtcct ctcttcaagc
     2341 aacagtatcc attgacagta gaagatcgaa tctatctggt gtcggcggat gatatccttt
     2401 tgattgaagc tatgcaagga aaactgatta tacaaacacc tgataaaaat tatgaaattg
     2461 atggctctct acaacaatgg caagataaac taccatcatc tcaatttgta cgggtacatc
     2521 gctcttacat tgtgaatatt aatgctatta aaacgattga accttggttt aaccaaacac
     2581 ttcagttaca cctttgtaat aaaataacag ttcctgttag cagagcaaat gtaaaacccc
     2641 taaaacaaat gttaggcata tctacctaac taatgaaaag ttattcatca taaaacttag
     2701 actattcatt tctaaaaaac aaccattcat cttgaaaata ggaaatttca atttttcaat
     2761 gctatactaa agtcatgaaa acgcttaata aaattaagca aaaaaatttt taggaggcag
     2821 tgatatggta acatttttag gaggggtagc ccttttaatt gttggttatt tcacgtacgg
     2881 tagatacatt gaaaagaatt ttcaaattga tgaaaatcgc caaacacctg cagaagcatt
     2941 aagggatgga tatgattttg ttcctatgcc aaaatggaag aatgggatga tcgagttatt
     3001 gaacatcgca ggaactggac ctatctttgg acctatttta ggagcactct atggtccagt
     3061 tgcttatatc tggatagtct taggatgtat ttttgcaggg gctgttcatg attatatgat
     3121 tggaatgatt tctcttagaa ataatggtgc atatcttcct gaattagcta gtcgctatct
     3181 tggaaaatca atgaagcatg tgatcaacat tttttcaatg ctattactta ttttagttgc
     3241 aaccgtattt gtggtaactc cagctaatct tatcctgagt attttaccag ctgggacact
     3301 atctttacct tggataatcg gtcttatttt cgtttattac cttatttcta cagtattacc
     3361 tattgataaa gcacttggta aagtttatcc tgtattctgc gtcatcctaa tggttagtac
     3421 agcagcagtt ggcttccgtt tattaactgg tggctttgat atgccaaact taacttttga
     3481 aacctttaaa aatatgcatc cagctggact tgggatattc cctgctttat tctttaccat
     3541 ttcatgtgga gccatctctg gtttccatgc tacccaagca cctatggtct caagaacaac
     3601 tgttaatgag agagaagggc gtttcacttt ctatggtatg atgattgcag aaggagtaat
     3661 tgcgatgatt tgggcaggtg cttcaatgag cttgttcaaa ggtcaaaatc tctatgaaat
     3721 gattgctgct ggaacacctt ctgcagttgt caatcaagta atgttaatgt tactcggtag
     3781 tgtaattgga actatcgcta tcattggtgt tattgttctc cctgtttcat ctggtttatc
     3841 agctttccgt agcttacgta ctatcgttgc tgattatatc catgtcaaac aagatactct
     3901 tccaaaaatc tttgctgtaa ctatcccatt atatgttatt tcatttgtcc ttacacacgt
     3961 tgattttaat ctcctatggc gttactttaa ctgggcaaat caagtaactg ctgttattgg
     4021 tttattagtt gctacaagat atctcatcct aaaaagacga aattattggg taacatttgt
     4081 accagctatg ttcatgttgt atgcagtagt tgtttatatt ttatctcaac caattggatt
     4141 taatatgggc ttaggcatct taacttattc acttgcttta gtgttaacag gtattcttgt
     4201 tggacttttc tggaaatctg gtcaaaaaca attaaaaact gttcatcctg aagcattcct
     4261 gtttaacgat catagaccaa ttaattatta cagtagttta gattcttaat attattttga
     4321 agccatgtta ttaagactta cagttatcac aataacctcg aatgaagtat cattcgaggt
     4381 tatttttcta ctaaaaatct cagtaaatat ttctatttga ttacaaatgt tacaaaaata
     4441 tttgacaacc ttctttctga aacttataat agatttgtcc ataacagagc aaaagagaga
     4501 gggatagtac atgagaaaac gttcaatttt gccaatatta gctgtcttat ttgttttcat
     4561 gagtatcttt agcgtatact ctatttttca tggtgataaa gctaaagaaa aagcaagtgt
     4621 cataaaacaa gcatctcaaa caagtcaaac ttcaaaaaaa gaggtcttac aaaagaagac
     4681 ttatccaaat ctaaataaat attcaaatct agaaattcat gtttcatcta cgaggcaaac
     4741 aatgacaata acctcgagtg acaaggtcat ctttaaaacg attgtttcaa caggagcaaa
     4801 agagagccct accccaaaag gaacttttgt catagaaccg gaacgaggag attttttcta
     4861 taacgcctca tcaaaagaag gagcctatta ttgggtctcc tttaaagagc atggtattta
     4921 tctctttcat agcgttccga ctgatcaaca aggaaatgag atcccagaag aagcaaagca
     4981 attaggaaaa gccgcctcac atggctgtgt gcgtatgtca agggcggatg caaaatggtt
     5041 ctatgaaaat attcctcaag gaacgactgt taccatcaaa tagtcttaat gtggtaaaaa
     5101 tcatgttatt aaaagcttgg agtaaacttc cagagctttt tcgtatatcc cttaaactca
     5161 attagtaaac gaaacctatc agcggaataa atgtataaag tatttcgata tcattaaaaa
     5221 aatgatagaa taatagtgtt aatcgaatat tttatcgacc tttatatgcg gttgaagaag
     5281 aataaaacta aaagattaga cctctaacaa atagatgtag acttttaagg tagtctatac
     5341 ataaggagaa tttatttcat ggctactatt caatggttcc caggacacat gtccaaagct
     5401 cgccgtcaag ttcaagaaaa tattaagcat gttgattttg ttacaatttt agtggatgca
     5461 aggttacctc tttctagtca aaatccaatg ctaaccaaaa tcgtcggaga caaacccaaa
     5521 ctaatgatcc tcaataaagc tgatctagca gatcccattc gaactaaaga atggagagat
     5581 ttttatgaat ctcaaggttt aaagacatta gctattaatt ctaaagaaca atcaacagtt
     5641 aaaaaagtaa ccgacatcgc taaaatatta atgtccgata aaatcgctaa actacgggaa
     5701 cgtggtattc aaaaagagac acttcgtacg atgattattg gaattccgaa tgcaggcaaa
     5761 tcgactctaa tgaatcgcct agcaggtaaa aaaattgcag tggttggtaa taaaccaggt
     5821 gtcacaaaag gtcagcaatg gctcaaatct aataaggaac tggaaatttt ggacactcct
     5881 ggtatcttgt ggcccaaatt tgaagatgaa ttagttggat taaagctagc attaacaggg
     5941 gcaatcaaag atcaattatt gcctatggat gaggtaacaa tatttggatt gaattacttt
     6001 aaaacctatt atcctgatcg tctcaaagag cgttttaaaa gtattaattt agaggatgaa
     6061 tctcctgaaa tcatcatgac cttaacacaa aaattaggtt atcgtgacga ttacgatcgc
     6121 ttttataatt tatttgtcaa agaagttcgc gatggtaaat taggacgcta tacgttagat
     6181 atcgtcggag aacatgatgg caacaattaa agaaatcaaa gcaattttag agactattgt
     6241 tgatttaaaa gacaaacgtt ggcaagaata tcaaacagat tcacgcgcag gtgttcaaaa
     6301 agcaatcctt caacgaaaaa aaaatattca gtcacattta gacgaagaag cgcgcttaga
     6361 acaaatgttg gtctacgaaa aaaaacttta cattgaacat ataaacctta ttgcaggaat
     6421 cgatgaagta ggtagaggtc ctttagcagg accagtagta gctgctgctg ttattcttcc
     6481 accaaactgt aaaataaaac atctcaatga ctccaaaaaa attccaaaga aaaagcatca
     6541 agaaatctat caaaatattt tagatcaagc gttagcagtt ggtattggta ttcaagacag
     6601 ccaatgtatt gatgatatca acatttatga agctacaaaa catgctatga ttgatgcagt
     6661 ttctcattta tctgtagttc ctgaacatct tttaattgat gctatggttt tggatctacc
     6721 aattccacaa actaaaatta tcaaaggtga tgctaattca ctctctattg cagcagcttc
     6781 aattgtagct aaagtgacca gagataaaat aatgtctgat tatgatagca aatatcctgg
     6841 atttgcattt tctaaaaatg ctggctatgg tactaaagag catttagagg gtttgcaaaa
     6901 atacggtatt actccgatac atcgtaaaag ttttgaacct ataaagtcaa tgttgtaaat
     6961 aatacggaag aggaaaagat gaccgaaaaa gaaaaaatgt tagcaggaca atattatcgt
     7021 ccatcagcac ctgaactacg caaggataga gaagttgcac taaaaaatat gcaagccttt
     7081 aacaatgaag atgatagtag caaacgtaac gtcattttac aaaaatggtt tggtgctact
     7141 gggaagagta tccatatgga acaacgtttt gtctgcgatt atggttgtaa tatttatgtt
     7201 ggtgaaaact tctacgccaa ttttaaccaa actttcttag atgtttgtga aattcgtatt
     7261 ggcgataact gtatgtttgg cccaaactgt caactcctca ccccactcca tcctctagat
     7321 ccaatagaaa gaaattcagg attagaatac ggtgccccaa tccaaatcgg gaataatgtc
     7381 tggctaggtg gaggagtgac tattttacca ggtgttgtat taggagataa tgtcgtcgtt
     7441 ggcgctggaa gtgtcgttac gaaatcattt gaaaataatg ttgttatagc aggaaaccct
     7501 gctaagataa tcaaaaaatt agcttaatgt aagaaaaaac ctttaatta
//
LOCUS       DBJYSH010000093         7202 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.093, whole genome shotgun sequence.
ACCESSION   DBJYSH010000093 DBJYSH010000000
VERSION     DBJYSH010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 7202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7202
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.093"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            141..821
                     /locus_tag="ACUBV3_001613"
     CDS             141..821
                     /locus_tag="ACUBV3_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HHU3848103.1"
                     /translation="MIKILLIEDDLSLSNSVFDFLDDFADVMQIFDGEEGLYEAESGV
                     YDLILLDLMLPEKNGFQVLKELREKGITTPVLIMTAKESIDDKGQGFDLGADDYLTKP
                     FYLEELKMRIQALLKRSGKFNDNSLIYGDIRVDMSTNSTFVNQTEVELLGKEFDLLVY
                     FLQNQNVILPKSQIFDRIWGFDSDTTISVVEVYVSKVRKKLKGTLFSENLQTLRSVGY
                     ILKHVETD"
     gene            805..2118
                     /locus_tag="ACUBV3_001614"
     CDS             805..2118
                     /locus_tag="ACUBV3_001614"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HHU3848104.1"
                     /translation="MSKPIKISKFKKNVSDSHFIHFFTVFSGIFLVMTVIILQVMRYG
                     VYSSVDSSLKYISTHPKNYINMVMSRTAAYLDNSNIASVKLKPGGQTVANTDIILFTS
                     EEEVINYFDAFSNYQFLKPNKKNLGGISELTLTNIFGQDETYHAVTVKVNNPAYPNVT
                     YMTAIVNIDQLVNAKERYEKIIIFVMTTFWIISIGASIYLAKWAQKPIIENYERQKAF
                     VENASHELRTPLAVLQNRLETLFRKPNATILENSENIASSLDEVRNMRILTTNLLNLA
                     RRDDGIKPELAVIKPTLFDSIFENYDLIAQENGKNFTGHNMIQDSFKTDKTLLKQLMT
                     ILFDNAIKYTDNDGSIDFTISETDKYLFLEIADNGPGISEEDKVRIFDRFYRVDKART
                     RQQGGFGLGLSLAQQIVNSLRGNITVIDNKPRGSIFKIKLPKVKG"
     gene            2207..2539
                     /locus_tag="ACUBV3_001615"
     CDS             2207..2539
                     /locus_tag="ACUBV3_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HHU3848105.1"
                     /translation="MEIEKTNRMNALFEFYAALLTDKQMNYIELYYADDYSLAEIAEE
                     SGVSRQAVYDNIKRTEKILEAYEMKLHMYSDYIVRSQIFDDILEKYTDDAFLQEKISI
                     LSSIDNRD"
     gene            2557..4122
                     /gene="ffh"
                     /locus_tag="ACUBV3_001616"
     CDS             2557..4122
                     /gene="ffh"
                     /locus_tag="ACUBV3_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HHU3848106.1"
                     /translation="MAFESLTERLQGVFKNIRGKKKLSEKDVQEVTKEIRLALLEADV
                     ALPVVKTFIKHVRERAVGHEIIDTLDPTQQIVKIVNEELTDLLGAETSEIEKSPKIPT
                     IIMMVGLQGAGKTTFAGKLANKLIKEDNARPMMIAADIYRPAAIDQLKTLGSQINVPV
                     FDMGTNHSAVEIVTKGLEQARENRNDYVLIDTAGRLQIDATLMQELHDVKAIAQPNEI
                     LLVVDSMIGQEAANVAEEFNRQLSISGVVLTKIDGDTRGGAALSVREITGKPIKFTGT
                     GEKITDIETFHPDRMASRILGMGDLLTLIERASQEYDEKRSMELAEKMRENTFDFNDF
                     IDQLDQVQNMGPMEDLLKMLPGMANNPAMKNFKVDENEIARKRAIVSSMTPEERENPN
                     LLNPSRRRRIAAGSGNTFVDVNKFIKDFNQAKQMMQGVMSGDMNKMMKKMGIDPNNLP
                     KDMPGMDGMDMSNLEGMMGQNGMPDLSSLGGDMDFSQMFGGGLKGKVGAFAAKQSMKR
                     MANKMKKAKKKRK"
     gene            4189..4875
                     /locus_tag="ACUBV3_001617"
     CDS             4189..4875
                     /locus_tag="ACUBV3_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SatD family protein"
                     /protein_id="HHU3848107.1"
                     /translation="MYLALIGDIINSKQILERETFQQSFQQLMTELSDVYGEELISPF
                     TITAGDEFQALLKQSKKVFQIIDHIQLALKPVNVRFGLGTGNIITSINSNESIGADGP
                     AYWHARSAINHIHDKNDYGTVQVAICLDDEDQNLELTLNSLISAGDFIKSKWTTNHFQ
                     MLEHLILQDNYQEQFQHQKLAQLENIEPSALTKRLKASGLKIYLRTRTQAADLLVKSC
                     TQTKGGSYDF"
     gene            4865..5638
                     /locus_tag="ACUBV3_001618"
     CDS             4865..5638
                     /locus_tag="ACUBV3_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3307 domain-containing protein"
                     /protein_id="HHU3848108.1"
                     /translation="MISDFLRDNPILTLLFCAHFLADFQWQSQSLADSKSHSWRGLWR
                     HLLIVFLPLAALMILIPETTLLNLSIWGSHIVIDSIKKLSYPWVEEGHFQKAAFIIDQ
                     LAHYTCIIVFYHALPTYLPPNHWLLPIKHFIVIALVFIIITKPINIVFKIFFNKFQAK
                     ELSSLLTQEKTKIMKEKSEDHEETIEGAGAMIGNLERLIMAILLISGQYAAIGLVFTA
                     KSIARYDKISKSQVFAEYYLIGSLFSIISVLITHWLLLV"
     gene            complement(5671..7188)
                     /locus_tag="ACUBV3_001619"
     CDS             complement(5671..7188)
                     /locus_tag="ACUBV3_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000093963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HHU3848109.1"
                     /translation="MSSFNRKKLKFLGISLATLTATTVTLVACGNESKNSGDNKVINW
                     YIPTEISTLDISKNTDAYSNLAIGNSGSNLLRIDKEGKPKPDLAKKVSVSSDGLTYTA
                     TLRDNLKWSDGSKLSAEDFVYTWRRIVDPKTASEYAYLATESHLLNADKINSGDIKDL
                     NKLGVTAKGNQVTFKLTSPCPQFKYYLAFSNFMPQKQSYVEKVGKDYGTTSKNQIYSG
                     PYLVKDWNGSNGKFKLVKNKYYWDSKHVKTNSVIVQTIKKPDTAVQMYKQGQIDFAEI
                     SGTSAIYQANKNNKDVVDASESLVPKKLAKLPNGEDLSKYTAPGYTYNTSKAQKLFKE
                     GLAEVGQSSLKLTITADSDSPAAKNDVDYVKSTWESALPGLTVEEKFVTFKQRLEDAK
                     NENFDVVLFSWGGDYPEGSTFYGLFTTNSAYNYGKFSSKEYDNAYQKAITTDALKPGD
                     AANDYKTAEKALFDQSYYNPVYYLGKKGLQNSKLKGLVRNSTGLNVDFTYAYKTE"
BASE COUNT         2418 a         1216 c         1277 g         2291 t
ORIGIN      
        1 ggattattca atctctattt ctttgtctgt gtcatgaaat tagttgacat aaaacatact
       61 ttatttagga tttattaagc aaagtttggt attctaatat taatagttca gataaaatat
      121 gtacatcgaa aggatatact atgataaaaa ttttattaat tgaagatgac ttgagtcttt
      181 ccaattccgt ttttgatttc cttgatgact ttgccgatgt catgcaaatt tttgatggag
      241 aagagggtct ttacgaagcc gaaagtggag tctatgatct gattcttcta gatctcatgt
      301 tacctgaaaa aaacggcttc caagtgttga aagagctacg tgaaaaaggt attacaaccc
      361 ctgtattaat aatgaccgct aaagaaagca ttgatgataa gggacaaggt ttcgacttag
      421 gtgctgatga ttatctaaca aaaccttttt acctagaaga actaaaaatg cgaattcagg
      481 ctcttttaaa gcgttctgga aaatttaatg ataacagcct aatttatggt gatattcgcg
      541 tagacatgtc tactaactca acctttgtta atcaaacaga agttgagctc ttaggtaaag
      601 agtttgatct tttagtctat ttccttcaaa atcaaaatgt tatcttaccg aaatctcaaa
      661 tctttgatcg tatttgggga tttgatagtg atacaacgat ttcagttgta gaagtctatg
      721 tctctaaggt tcggaaaaaa ttaaaaggaa cacttttttc agaaaacctt caaactttgc
      781 gtagtgtagg atatatttta aagcatgtcg aaaccgatta agataagtaa attcaaaaaa
      841 aatgtctcag atagtcattt tatccatttt ttcacagttt ttagtggtat ttttctggtt
      901 atgactgtta tcatcctcca agtcatgcgc tatggtgttt attcttctgt tgatagtagt
      961 ttaaagtata tcagtaccca tcctaaaaat tacatcaata tggttatgtc acgtactgct
     1021 gcctacctag ataactccaa tatcgcaagt gtcaagttaa aacccggcgg tcagacagta
     1081 gctaatactg atattattct ttttacatca gaagaagaag tcattaacta ttttgacgca
     1141 ttttcaaact atcagttttt aaaacctaat aaaaaaaatc taggtggcat tagtgaatta
     1201 actttaacca acatctttgg tcaagatgag acatatcatg ctgtcaccgt aaaagttaat
     1261 aaccctgctt atcctaatgt gacttacatg acagctattg ttaatattga tcaattagta
     1321 aatgctaaag aacgctatga aaaaattatt atatttgtca tgactacttt ttggattatt
     1381 tcaattggag caagtatcta cttagctaaa tgggcacaaa aaccgattat agaaaactac
     1441 gaaagacaga aggcttttgt cgaaaatgct agccacgagc tgcgtactcc cctagctgtc
     1501 ttacaaaatc gcctagaaac gctattcaga aaaccgaatg ccaccatttt agaaaatagt
     1561 gaaaatatcg cttcaagtct agatgaagta cgaaatatgc gtattttaac cactaatctt
     1621 ttaaacttag caagacgtga tgatggaatc aaaccagaat tagctgttat taagcctaca
     1681 ctttttgata gtatctttga gaattatgat ctaatcgccc aagagaatgg taaaaatttt
     1741 actggacata atatgatcca agatagtttc aaaacagata agacactgct aaaacaactc
     1801 atgactattt tatttgataa tgctataaaa tatactgaca atgatggtag cattgacttt
     1861 accatatctg aaactgataa gtatctcttt ttagaaatcg ctgataatgg accaggaatt
     1921 tcagaggagg ataaagtcag aatttttgat cgtttttacc gtgttgacaa agcaagaaca
     1981 cgacaacaag gagggtttgg cctcggcctt tctttagctc aacaaatcgt taactcactg
     2041 cgaggtaata ttacagtaat tgataataaa cctagaggca gtatttttaa gataaaacta
     2101 ccaaaagtaa aaggttaggc catgttagtc ctaacctttt aaattttaaa gtggtcttgc
     2161 atacttgata tagcaagctg ataggaaatt tggtataata aaagctatgg aaattgaaaa
     2221 aacaaatcgt atgaatgccc tttttgagtt ttatgcggca ttattaacag ataaacaaat
     2281 gaattatatc gagttatact acgccgatga ctacagcctt gctgaaatag ctgaagaatc
     2341 cggtgttagc cgacaagctg tctatgataa tatcaaacga actgaaaaaa ttcttgaggc
     2401 gtacgaaatg aaactacata tgtattcaga ttatattgtc cgtagtcaga tttttgatga
     2461 cattctagaa aaatataccg atgatgcctt tttacaagaa aaaatttcca ttctaagtag
     2521 cattgataat agagattaaa tgaggagaaa taatttatgg ctttcgaaag tttaacagag
     2581 cgcctgcaag gagtttttaa aaatattcgt ggtaagaaaa agttatctga aaaagatgtg
     2641 caagaagtca ccaaggagat tcgtctagct cttttagaag ctgacgttgc tcttccagtt
     2701 gttaaaacct ttattaaaca tgttcgtgaa cgtgctgttg gacatgaaat cattgataca
     2761 ctagatccta cgcaacaaat tgttaagatt gtcaatgaag agctaacaga tttattaggg
     2821 gctgagacct ctgaaattga aaaatcccct aaaattccaa ccatcattat gatggttggt
     2881 ctacaaggtg ctggtaaaac aacttttgcc ggtaagttag caaacaaact aatcaaagaa
     2941 gacaatgctc gtcctatgat gatagcagct gatatttatc gaccagctgc tattgatcag
     3001 ttaaaaaccc ttggtagcca gattaatgtt cctgtctttg atatgggaac taaccattca
     3061 gcagttgaga ttgtcactaa aggtttagaa caggctcgtg aaaatcgtaa tgactatgtt
     3121 cttattgata cagccggacg cctacaaatt gatgcaaccc ttatgcagga attgcatgat
     3181 gtcaaagcca tagctcaacc taatgaaata ctccttgtcg ttgacagcat gattggacag
     3241 gaagctgcaa atgtcgctga agaatttaac cgtcagctca gcattagtgg tgttgttctg
     3301 acaaagattg acggtgatac acgcggtggt gctgcccttt cagtacgcga aattactgga
     3361 aaaccaatta aatttacagg gacaggggaa aaaatcactg atattgaaac tttccatccc
     3421 gatcgtatgg caagccgtat attggggatg ggagacctgc taacacttat tgagcgcgct
     3481 agtcaagaat acgacgaaaa acgttcaatg gaattagcgg agaaaatgcg tgaaaacacc
     3541 tttgacttca acgattttat tgatcaatta gatcaagttc aaaatatggg gccaatggaa
     3601 gatctcctta aaatgcttcc aggaatggct aacaatccag caatgaaaaa cttcaaagtg
     3661 gatgaaaacg aaattgctcg caaaagagcc attgtgtcat caatgactcc agaagagaga
     3721 gaaaatccaa atctcctcaa tccaagtcgt agacgtcgta ttgctgctgg atcaggtaat
     3781 actttcgttg atgttaataa attcattaaa gattttaacc aggcaaaaca aatgatgcaa
     3841 ggcgtgatgt ccggtgatat gaataaaatg atgaaaaaaa tgggaattga tcctaataac
     3901 ctccctaaag atatgcctgg aatggacgga atggatatgt ctaatttaga aggtatgatg
     3961 gggcaaaatg gcatgcctga cttatctagt ttaggtggcg atatggactt cagccaaatg
     4021 tttggtggtg gcttaaaagg aaaagtagga gcctttgcag ctaaacaatc aatgaaacgt
     4081 atggctaata aaatgaagaa agctaagaag aaacgcaaat aaaaatgtct atttaaatag
     4141 acatttttat ttttgtctgc tataatagac aaaaaggtgg tgatatttat gtatttagca
     4201 ttaatcggtg atatcattaa ttcaaaacag atacttgaac gtgaaacttt ccaacagtct
     4261 tttcagcaac taatgaccga actatctgat gtatatggtg aagagctgat ttctccattc
     4321 actattacag ctggtgatga atttcaagct ttattgaaac aatcaaaaaa ggtatttcaa
     4381 attattgacc atattcaact agctctaaaa cctgttaatg taaggttcgg cctcggtaca
     4441 ggaaacatta taacatccat caattcaaat gaaagtatcg gtgctgatgg tcctgcctac
     4501 tggcatgctc gctcagctat taatcatata catgataaaa atgattatgg aacagttcaa
     4561 gtagctattt gccttgatga tgaagaccaa aaccttgaat taacactaaa tagtctcatt
     4621 tcagctggtg attttatcaa gtcaaaatgg actacaaacc attttcaaat gcttgagcac
     4681 ttaatacttc aagataatta tcaagaacaa tttcaacatc aaaagttagc ccaactggaa
     4741 aatattgaac ctagtgcgct gactaaacgc cttaaagcaa gcggtctgaa gatttactta
     4801 agaacgagaa cacaggcagc cgatctatta gttaaaagtt gcactcaaac taaaggggga
     4861 agctatgatt tctgattttt taagggataa tccaatccta actttattat tttgcgcaca
     4921 ttttttagct gactttcagt ggcaaagtca atccttagca gatagtaaat cacatagttg
     4981 gaggggactc tggagacatc tcttgattgt tttcctcccc ctagcagctt taatgatatt
     5041 gataccagaa actactttgt taaatttaag catatgggga agtcatattg ttattgatag
     5101 tataaagaaa ttgagttacc cttgggtaga ggaagggcat ttccaaaaag cagcttttat
     5161 tatagaccaa ctagcccact atacttgcat tatcgttttt taccatgcgc ttcctacata
     5221 tttaccgcct aatcattggc ttttgcctat caagcacttt attgtcatag ctctggtttt
     5281 tatcattatc accaaaccca ttaatattgt gtttaaaatt ttctttaata aatttcaagc
     5341 taaagaatta tcatcattat taacacaaga aaaaacaaaa ataatgaaag aaaaatctga
     5401 agatcatgaa gaaacaattg aaggagcagg agctatgatt ggtaatttag agcgtctaat
     5461 tatggctata ttactaatct caggacagta tgctgctata ggtcttgttt ttactgcaaa
     5521 atccattgca cgttacgata aaatttctaa aagtcaggtt tttgccgaat attatttaat
     5581 tggatcattg ttctcaatta taagtgtttt gataactcat tggttacttt tggtatagaa
     5641 aagcgctaga aatatcctaa cgcttttctt ttattctgtc ttataagcat aagtaaaatc
     5701 aacattcaaa ccagttgaat ttcttaccaa cccctttaat ttagaatttt ggagtccttt
     5761 tttccctagg taataaactg ggttataata tgattgatca aataaagctt tttcagcagt
     5821 tttgtaatca ttagcggcat ctcctggttt caaggcgtca gtagtaattg ccttttgata
     5881 ggcattgtca tactctttag aagaaaattt accataatta tacgcagaat tagtcgtgaa
     5941 taacccatag aaagttgatc cttctggata atctcctccc catgagaaga gcacgacatc
     6001 aaaattttca ttttttgcat cttcaaggcg ttgcttgaaa gtaacaaatt tttcttcaac
     6061 agttagacca ggcaaagcac tctcccatgt agatttcaca tagtcaacat catttttagc
     6121 tgctggagag tccgaatcag ctgtgattgt taacttgaga gatgactgac caacctcagc
     6181 aagaccttct ttaaaaagtt tttgagcttt acttgtatta tacgtataac ctggggcagt
     6241 atacttggaa agatcctcac cattaggtaa tttggctaat tttttaggga ccagagattc
     6301 agacgcatct actacatctt tattattttt attagcttga taaatagcag aagttcctga
     6361 tatttcagca aaatcaatct gaccttgctt gtacatttga actgcagtat ctggtttttt
     6421 gatagtttga actatgacac tatttgtttt aacatgttta gaatcccaat aatatttatt
     6481 tttgactaat ttaaatttac catttgaacc attccaatcc ttaacaaggt aaggacctga
     6541 gtaaatttga ttttttgatg ttgtaccata atctttacca actttttcaa catacgattg
     6601 tttttgaggc ataaaattag agaaggctaa ataatattta aattgagggc atggacttgt
     6661 caatttaaag gtcacttgat tgcctttagc tgttacacca agtttattta aatctttaat
     6721 atctccagaa ttaatcttat cagcatttaa aaggtgcgat tctgtcgcca agtaagcata
     6781 ttcagatgca gttttaggat caactattct ccgccatgtg taaacaaaat cttcagcgct
     6841 tagtttgctt ccatctgacc attttaaatt atcacgaagt gtagcagtat aagtaagacc
     6901 atcagaagaa acggatactt ttttagctaa atcgggtttt ggtttgccct ctttatcaat
     6961 tctaagtaaa ttgctgcctg aattacctat agctaagttt gaataagcat ctgtattctt
     7021 tgaaatgtcc aatgtactga tttcagtagg aatataccaa ttaataactt tattatctcc
     7081 agagttcttt gactcattac cacaggcaac cagggtaact gtggtggctg ttagagtcgc
     7141 caaagatata cccaaaaact taagtttttt tctattaaaa ctagacataa taccagtcct
     7201 cc
//
LOCUS       DBJYSH010000094         7138 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.094, whole genome shotgun sequence.
ACCESSION   DBJYSH010000094 DBJYSH010000000
VERSION     DBJYSH010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 7138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7138
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.094"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            complement(158..352)
                     /locus_tag="ACUBV3_001620"
     CDS             complement(158..352)
                     /locus_tag="ACUBV3_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3848110.1"
                     /translation="MIMSGQRATSTIQSDINIFHHIEAICISKSNSIGLPAIQNHLGQ
                     LIQRERLGLFFSITYSKAPT"
     gene            351..545
                     /locus_tag="ACUBV3_001621"
     CDS             351..545
                     /locus_tag="ACUBV3_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001878969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3848111.1"
                     /translation="MLFLMIKPDYIRYFKVIDDMHDTMYQQTVFHVQLQAWKYTMKSI
                     DQLVVDDLNHISNEKTTLVK"
     gene            complement(596..772)
                     /locus_tag="ACUBV3_001622"
     CDS             complement(596..772)
                     /locus_tag="ACUBV3_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3848112.1"
                     /translation="MSKKTDSNYLHSNCSDKKIWSDEAPLQQETIPKDRDCVIKPVIT
                     MTVEHVEIPLDDQS"
     gene            complement(807..2396)
                     /locus_tag="ACUBV3_001623"
     CDS             complement(807..2396)
                     /locus_tag="ACUBV3_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HHU3848113.1"
                     /translation="MKIKKIISGFAAALIISSLSTINYEVKADDTTSEYHYISKQNNE
                     KQLISYIKEQHRLLNQFVVDNVNSFTQLNANPTIEQLNRAITLFKQKDEQLFNQVKAG
                     HLSPSNYNAIVNQRNVINQTVQNLIDQNHNKIQTSQNKAAQLVGQRNQVVNKIQAILA
                     TVNYNSVNSIQEAENLFHSLRNQIEPLVAEVNNYKAAMAILQQEVDALSTAAIETETS
                     KLATLKVSENTSVPANKVEEKTTQSEASGNKQEVTKSEEKQATSDAKASQPESANIAD
                     YDSLKEVLRNNISNQVPHISVQMEFKTQEQVDEYQKNLGSIIREIGDTLGTATEFNAK
                     SNISTYTLGGQIQRIIVKSDITITYTLKGDMVGLHKEYKQFVDSFVKENITNKNITSD
                     YEKAKVIHDHLVNNYTYATEELATTRETASGISIHAPEALYKDKRGVCQAFAVMFKDM
                     AAAGLSVWYVTGQAGGGNHAWNIVTINGVKYYVDTTWDNNIKSNKYFLVGKTIMDADH
                     LLDSQYNALAKDIPADRHQGA"
     gene            complement(2457..2525)
                     /gene="shp2"
                     /locus_tag="ACUBV3_001624"
     CDS             complement(2457..2525)
                     /gene="shp2"
                     /locus_tag="ACUBV3_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_128650799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide pheromone SHP2"
                     /protein_id="HHU3848114.1"
                     /translation="MKKINKALLFTLIMDILIIVGG"
     gene            2613..3461
                     /gene="rgg2"
                     /locus_tag="ACUBV3_001625"
     CDS             2613..3461
                     /gene="rgg2"
                     /locus_tag="ACUBV3_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system transcriptional regulator
                     Rgg2"
                     /protein_id="HHU3848115.1"
                     /translation="MEKELGKTLRRLRKGKKVSISSLADEHLSKSQISRFERGESEIT
                     CSRLLNILDKLNITIDEFVSIHSKAHTHFFILLNRVRKYCAEKNVTKLVALLEDHNHK
                     DYEKIMIKALIFSIDQSIEPNQEELARLTDYLFTVEQWGYYEIILLGNCSRLINYNTL
                     FLLTKEMVNSFAYSEQNKTNKILVTHLAINCLIISIDHSYFEHSHYLIDKVRSLLQDE
                     VNFYEKTVFLYVTGYYHLKLGDTSSGKEDMRKALQIFKYLGEDSFYNTYNEHYCQKVL
                     GNNKEC"
     gene            3610..4431
                     /gene="mutM"
                     /locus_tag="ACUBV3_001626"
     CDS             3610..4431
                     /gene="mutM"
                     /locus_tag="ACUBV3_001626"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HHU3848116.1"
                     /translation="MPELPEVETVRKGLERLVVNQEIASITIKVPKMVKTDLNDFMIS
                     LPGKTIQQVLRRGKYLLFDFGEMVMVSHLRMEGKYLLFPNKVPDNKHFHLYFKLTNGS
                     TLVYQDVRKFGTFELVRKSSLKDYFTQKKLGPEPTADTFQFEPFSKGLANSKKPIKPL
                     LLDQRLVAGLGNIYVDEVLWAAKIHPQRLANQLTESETSLLHKEIIRILTLGIEKGGS
                     TIRTYKNALGEDGTMQKYLQVYGKTGQPCPRCGCLIKKIKVGGRGTHYCPRCQCL"
     gene            4422..5015
                     /gene="coaE"
                     /locus_tag="ACUBV3_001627"
     CDS             4422..5015
                     /gene="coaE"
                     /locus_tag="ACUBV3_001627"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017770961.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HHU3848117.1"
                     /translation="MLMTKIIGLTGGIASGKSTVTKIIRESGFKVIDADQVVHKLQAK
                     GGKLYQALLEWLGPEILDADGELDRPKLSQMIFANPDNMKTSARLQNSIIRQELACQR
                     DQLKQTEEIFFMDIPLLIEEKYIKWFDEIWLVFVDKEKQLQRLMARNNYSREEAELRL
                     SHQMPLTDKKSFASLIIDNNGDLITLKEQILDALQRL"
     gene            5139..5840
                     /locus_tag="ACUBV3_001628"
     CDS             5139..5840
                     /locus_tag="ACUBV3_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HHU3848118.1"
                     /translation="MELVIRDIRKRFQETEVLRGASYRFYSGKITGVLGRNGAGKTTL
                     FNILYGDLAADNGTICLLKDNHEYPLTDKDIGIVYSENYLPEFLTGYEFVKFYMDLHP
                     SDDLMTIDDYLDFMEIGQTERHRIIKGYSDGMKSKLSLICLMISKPKVILLDEPLTAV
                     DVVSSIAIKRLLLELSEDHIIILSTHIMALAEDLCDIVAVLDKGKLQTLDIDRKHEQF
                     EERLLQVLKGDEYDK"
     gene            5830..7138
                     /locus_tag="ACUBV3_001629"
                     /pseudo
     CDS             5830..7138
                     /locus_tag="ACUBV3_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200920.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2304 a         1078 c         1302 g         2454 t
ORIGIN      
        1 tgagaaaata tagtatgacg aattcctaaa taaaaaataa tgaaaggaac atgatgttaa
       61 aagatgtgaa tgttggaatg agtcatttat tagttagagg tgctattatt ctagtagtag
      121 ggattgtttt tctgttgcaa cagattcagt aagtacttca agtgggagct ttggaatagg
      181 ttatcgagaa gaaaagaccc agacgttctc tttggatcag ctgtcccaag tgattttgta
      241 ttgcggggag cccaatactg ttagatttac ttatacaaat agcttctata tgatgaaaga
      301 tatttatatc gctctggatg gtgctggtag ctctttgacc actcatgatc atgctgttcc
      361 taatgattaa accagattat ataaggtatt tcaaagtcat tgacgatatg catgatacca
      421 tgtaccaaca gactgttttt catgttcaac tgcaagcttg gaaatacacc atgaaatcga
      481 ttgatcaatt agttgtagat gatttgaatc atatcagtaa tgagaaaacc actttggtta
      541 aataaaaagg aacactgtca aaaatggcag tgttcctttt tattttagga gagagctaag
      601 attggtcatc taatgggatt tccacatgtt caactgtcat agtaatgaca ggtttgataa
      661 cacaatctct gtctttaggg atagtctcct gttgcagagg cgcctcatcg ctccaaattt
      721 tcttgtcaga acaattggaa tgtagataat ttgaatcagt ctttttagac atttcatatc
      781 cttccaggct caatacaatt tttgagttat gcaccttgat ggcgatcagc tggaatatct
      841 ttagctaatg cattgtattg actatccaaa agatgatcag catccattat tgttttacca
      901 acaaggaaat atttattgct ttttatatta ttatcccatg ttgtatcaac ataatattta
      961 acgccattaa tagtaacaat gttccaagcg tgatttccac ctccagcttg accagttaca
     1021 taccatactg ataagccagc agcagccata tctttaaaca ttactgcaaa ggcttgacaa
     1081 acaccacgtt tatctttgta gagtgcttca ggagcatgga tactgatacc actagcagtt
     1141 tcacgagtgg ttgccagttc ttcagtcgcg taagtgtaat tattaaccaa gtggtcatga
     1201 attactttag ctttttcata atcacttgtg atatttttgt tagtaatatt ttctttgaca
     1261 aaagaatcta caaactgttt atattcttta tgtaatccta ccatgtcacc tttaagagta
     1321 taggtgattg tgatgtcgct ttttacaata atgcgttgga tttgtccacc aagagtataa
     1381 gtgctaatgt tacttttggc attgaattca gttgctgttc caagtgtatc tccaatttcc
     1441 cggatgatgc ttccgagatt tttttggtat tcgtcaactt gttcttgagt tttaaactcc
     1501 atttgaacac tgatgtgtgg tacttggttg ctaatattat ttcgtaaaac ttcttttaaa
     1561 ctatcgtaat cggcaatatt agctgactca ggctgtgatg cctttgcatc agaggtagcc
     1621 tgtttttcct cactcttagt tacttcttgt ttattgcctg acgcttctga ttgagtagtt
     1681 ttttcttcta ctttgtttgc aggaacagaa gtattttcgc taactttgag agtagcaagt
     1741 ttagaagtct cagtttcaat agccgctgtt gatagggcat ctacttcttg ttgaaggatt
     1801 gccatagcag ctttgtaatt attaacttca gctacaagag gttcaatttg atttctgagt
     1861 gaatgaaata aattttcagc ttcttgtata gaattcacag agttgtagtt tacagttgct
     1921 aaaatagctt gaattttgtt aacaacctga ttacgttgcc ccacgagctg agctgcttta
     1981 ttttgacttg tctgaatctt attatgattt tggtcaatca gattttgaac agtttggtta
     2041 atgacattac gttgattaac gatagcatta tagttactgg gagagagatg accagctttc
     2101 acctggttaa ataattgctc atctttttgt ttaaataatg ttatagctct atttaactgt
     2161 tcaatagttg gattagcatt tagttgagtg aatgagttga cattatcaac aacaaattga
     2221 ttgagcaaac gatgttgttc cttgatgtaa ctaataagct gcttttcatt attttgctta
     2281 ctgatatagt gatactcact ggtggtgtca tcagctttaa cctcatagtt aatagttgat
     2341 agtgaactga taattaaagc tgcggcaaag ccactaataa tttttttaat cttcattagt
     2401 tctcctgtta taaactaaat aaaatgggat tccataacaa ccatttgtgg ggagttttat
     2461 ccaccaacga taattaggat atccatgatt aatgtgaaca acaaagcttt gttaattttt
     2521 ttcatgacta tctcctttct gattttctat tttgtacttg tattatataa tggatttaaa
     2581 gggtctttta aattgttgtg aaagtgggaa aaatggaaaa agaattagga aaaacactaa
     2641 gaagattacg taaaggaaaa aaagttagta tttcttcttt agcagatgag cacctatcca
     2701 agtcacaaat ctcaaggttt gagagaggag agtcagaaat tacttgtagt cgtttgctta
     2761 atattttaga caaactcaac atcactattg atgaatttgt gtcgattcat tctaaggcgc
     2821 acactcattt ttttatcctt ttaaatcgag taagaaaata ttgtgctgaa aaaaatgtaa
     2881 ctaaattagt ggctctctta gaggatcata accataaaga ctatgaaaag attatgatta
     2941 aggctcttat tttttcaatt gatcaatcaa tagagcctaa tcaagaagaa ttagcacgct
     3001 taacagacta tctttttacg gtggagcagt ggggatatta tgaaattatt ctcttaggaa
     3061 attgctcacg tctcattaat tacaatactt tatttttatt gaccaaagaa atggttaatt
     3121 cgtttgctta ttcagaacaa aataaaacca ataaaatcct agttacacac ttagcaatta
     3181 attgtctaat catcagcatt gatcactctt actttgagca tagccactac ttaattgata
     3241 aggttagatc attgttacag gatgaagtta atttctatga aaaaactgtt ttcttatatg
     3301 taactggata ctatcattta aaacttggtg atacttcttc aggaaaagaa gatatgagga
     3361 aagctttaca gatttttaaa tatttaggtg aagattcatt ttacaatact tataatgaac
     3421 attattgtca aaaagtactt ggcaataata aagaatgcta atgagtgcaa gtttatgagt
     3481 aatttttaaa gcttttgtct caaaatgata ataagaaagg aaaatagaaa tcgaatggtc
     3541 aggtgacatc aagaacgttt ggaaattatt gctctaagca atgactattg atttttagac
     3601 tatatattta tgccagaatt accagaagta gaaaccgtta gaaaaggact agaaagactt
     3661 gtagttaatc aagaaatagc ttctataacg attaaagtcc ctaaaatggt gaaaactgat
     3721 ttgaatgact ttatgataag tctaccagga aaaacaattc aacaggtatt gagacgaggg
     3781 aagtatcttc tttttgattt tggtgaaatg gttatggtat cacatttacg catggaagga
     3841 aaatatctac tcttcccaaa taaagtacca gataacaaac attttcacct ttatttcaaa
     3901 ttgactaatg gcagcacatt ggtttaccaa gatgtccgta aatttgggac atttgagttg
     3961 gttcgtaaat catctctcaa agattatttt actcaaaaaa aattaggtcc tgagccaact
     4021 gctgacacat ttcaatttga gcctttttca aaaggcttag caaactccaa aaagccaata
     4081 aaacctttac tattagatca gagattagta gcaggacttg gaaatattta cgtagatgaa
     4141 gtcttgtggg cagcaaaaat tcacccacag cgtttggcta atcaattgac cgaatctgaa
     4201 acaagcttat tacacaagga aattattcgc attttaactt taggcatcga aaaaggtggc
     4261 tccacgatta gaacatataa gaatgctctt ggagaggacg gtacgatgca aaagtattta
     4321 caggtctatg gtaaaactgg gcaaccgtgt ccaaggtgtg gttgtttaat taagaagata
     4381 aaagtaggag ggagagggac gcattattgc cctcggtgtc aatgcttatg acaaaaataa
     4441 taggactgac aggagggata gcttctggaa agtcaacggt aacaaaaata atacgagaat
     4501 caggttttaa agtcatagat gcggatcaag tggttcataa attgcaagct aagggtggga
     4561 aactttacca agctttatta gaatggttgg gtcccgagat acttgatgct gatggtgagt
     4621 tggatagacc aaagctttct caaatgattt ttgctaatcc agacaatatg aagacatcag
     4681 ctaggctaca aaatagtatc attcgtcaag agttagcatg tcagcgcgac caattaaaac
     4741 aaacagaaga gatatttttc atggatattc ctttattgat tgaagaaaag tatataaaat
     4801 ggtttgatga gatttggttg gtatttgttg ataaagaaaa acaattacaa cgattaatgg
     4861 cccgtaacaa ctacagtcga gaagaagcag aattacgact ttcacaccaa atgcctttaa
     4921 cagataaaaa aagtttcgct agtcttatta ttgacaataa tggtgattta ataactttaa
     4981 aagagcaaat attggatgct cttcaacgtt tatagaacta ctaaatggat aatgtattta
     5041 tccatttttt gtctttatta cactagtttt aaaaaacttg cgattaaacc acagtaatag
     5101 tacaatgata taaaaggttt actttcaaag gagtttttat ggagttagta attagagata
     5161 ttcgtaagcg gtttcaggaa acagaggtct tgagaggagc aagttaccga ttttattcag
     5221 gtaaaataac aggggtctta ggtaggaatg gtgctgggaa aacaacttta tttaatatac
     5281 tttatgggga tcttgcagct gacaacggga ccatttgttt attgaaggat aatcacgagt
     5341 atcctcttac cgataaggat attggtattg tttattccga aaactacctt ccagaatttt
     5401 taacagggta tgaatttgta aaattttaca tggatttaca tccttcagat gatttaatga
     5461 caatagatga ttatttagat tttatggaaa taggacaaac agagcgtcat agaattatca
     5521 aaggatattc tgatggaatg aagagtaagc tctcattaat ttgcctgatg atttctaagc
     5581 caaaagtaat tttactagat gagccactga ctgcagttga tgttgtatca agtattgcaa
     5641 taaaacgcct tttgttggaa ttaagtgagg atcatattat tatattatca actcatataa
     5701 tggccttagc agaagatcta tgtgatattg tggctgtatt agacaaagga aaactccaaa
     5761 cattagatat tgatcgtaaa catgaacaat tcgaagagcg tcttcttcaa gtgttgaagg
     5821 gagatgaata tgacaagtaa agcattaata tggtctatat tacagattga tagtgctaga
     5881 gcttataata gttttttata ttaccttcag aaaatcccct ggttgggaag aaaaatccct
     5941 aaccgatggt ataaaaggtg tagtactatt tattcgtatc attttctttt tcttaagagg
     6001 ttttggtcgt tccataggtt acgtactata ttgttatgtt ttatcatttt ttgttagaag
     6061 tggcatgaag cagttaaata ttttaccgca agaaggtttt accctaactt taacattgat
     6121 ggtgattacc tctcttttaa ttgtcataaa tttctatata aaacttatag aacctaatga
     6181 tatgcaacaa actcatatga ttcgcctttt cagaattgag ccatcgaggt atttccaatc
     6241 cgctgaactt ttagaagcca gtagtgaact attttttaga agtttgagct ttggcatatt
     6301 tttccattta aatcactttg ctttttggca tggtttgact tttgctttat ttttagcagg
     6361 tagtcgcctt ggaatcaagg tgctctgttt acctttatat ggtagaggta aagaccaaga
     6421 gaatccagag tctttactaa atattttatt ttacttaggg attggtatag gagtcttcct
     6481 ttctctcttt ttccttcttc ttggcaaaca gttctctcca tatccatttt tttcatggta
     6541 caccggtatt gcaggactta tcatatggtt agttagttac tatcgattaa aaacttattc
     6601 taaacttaac cacttgacct accttaccct aacgcatgag acgatgaagg agaaaattgc
     6661 aaagattgat aatatagagc tgcttgggat tgaaatgaaa gatagggata taaatgtcag
     6721 tgaactttct cccctgctat ttgaaaattt atcaggtatt tcatatttaa acgctatctt
     6781 tttaaaacgg atgagtagat acttgcaaag gaaaattttg gtacgtttag ctgttttaag
     6841 tgttctgttt ggaatagaac tccttttcct gctatttttt aaagaaaaga taaatattcc
     6901 gatgaatgag catagttttt caaaattcct atttttgagt gcagttccag gatatctaat
     6961 atatatcggt gaatcctatt caaaattttg tttctatcat ttggatcgtc cattattaag
     7021 atataatttc tatcgtgaaa gagaaaatat cttagcaacc ttaaaaatac gtttccttta
     7081 tagtataaaa cttaatttcc ccatttttgt agctttaaac atatgttttg gaacctat
//
LOCUS       DBJYSH010000095         7057 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.095, whole genome shotgun sequence.
ACCESSION   DBJYSH010000095 DBJYSH010000000
VERSION     DBJYSH010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 7057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7057
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.095"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            61..1023
                     /locus_tag="ACUBV3_001630"
     CDS             61..1023
                     /locus_tag="ACUBV3_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HHU3848119.1"
                     /translation="MKKLLTTPVMGCTLVILSLTSLFVGVKSIPLEQITHLNQSQVDI
                     FLASRLPRTISILISGASLSVCGLLMQQLTQNKFVSPTTSGTMDWAKLGVVVTLIFFK
                     NTSIFIQLCIASGFAILGSLLFVTILKMITFKDNIFIPLIGLMLGQIVAAATVFLGTH
                     FQVLQSVNSWLQGNFSIMTSHRYEILYLALPCLFLVYFFAHQFTIVGLGESFAKNLGI
                     AYEKMIYFGLVLVSIMTSLVIIIVGALPFLGLIVPNLISLTKGDHMSSTILETSLLGA
                     CIVMICDLFGRLVIFPYEVSIGVTLGVLGSAFFLISIIRNEGKS"
     gene            1020..1994
                     /locus_tag="ACUBV3_001631"
     CDS             1020..1994
                     /locus_tag="ACUBV3_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HHU3848120.1"
                     /translation="MIKDLEIPKLLILLILLIATIILFLIYGIPTDANEFLIIYILKT
                     RYQKLIALILVGICIGSSSLIFQTLTNNRLLTPSIIGLDSLYVLIQTGLMYLIGAQRV
                     IKFSSFSSFLLSLLLMVGFAYLLFTILFRNKKQSLYFVLLAGLIFNTLFSSISSFIQA
                     IMDPNDFMILQNQLFASFNAINTKILWISFIIIVVSFVINWPFIKELDVLLLGKENAI
                     SLGISYQKLTTRIFLWLALMVAIATALVGPITFLGLLVAHITYHSFHTFRHQILVPIA
                     IVICIFTLVLGQHLVQNLLHLTVQLSVLLNLIGGSYFIFTLIKGRKNL"
     gene            1991..2752
                     /locus_tag="ACUBV3_001632"
     CDS             1991..2752
                     /locus_tag="ACUBV3_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000614752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HHU3848121.1"
                     /translation="MIQINNLHKFYGQKEILKDINISIPKGKVTAILGPNGSGKSTLL
                     SCISRLEPYDNGEIFLDKVPLAHYSSNDLAKTLAILRQSNHLTLKIKVSDLIGFGRFP
                     YSKGRLSQKDKAVIESVISYMDLNDIADEFINNLSGGQIQRAFIAMTMAQDTQYICLD
                     EPLNNLDMKYAVQMMDLIKRYAYEFNKTIVIIIHDINFATHYADNVVALKEGQVVSCG
                     TVEDVMQEKILSHLFDMPIRIETVDGKPIILFCTT"
     gene            2814..3842
                     /locus_tag="ACUBV3_001633"
     CDS             2814..3842
                     /locus_tag="ACUBV3_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HHU3848122.1"
                     /translation="MTKKLIIAILALCTILTTSQAVLAKEKSQTVTIKNNYSVYIKKE
                     KRDKPDNKKQISETLKVPLKPKKVVVFDMGALDTITALGAEKSVIGIPKAKNALSLLP
                     NNIKSVYKAKRYQDVGSLFEPNFEAIARMQPDVVFLGARMASVDNIEKLKEAAPKAAL
                     VYAGVDSKKVFDKGVAERVTMLGKIFDQNKKAKTFNKDIAQAVLKLQKTIEKKGKPTA
                     LFVMANSGELLTQSPSGRFGWIFSVGGFKAVNENEKLSSHGTPVSYEYIAEKNPNYLF
                     VLDRGATIGQGASSKELFNNDVIKATDAVKNKRVHEVDGKDWYINSGGSRVTLRMIKD
                     VQNFVDNR"
     gene            3980..4822
                     /gene="dprA"
                     /locus_tag="ACUBV3_001634"
     CDS             3980..4822
                     /gene="dprA"
                     /locus_tag="ACUBV3_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HHU3848123.1"
                     /translation="MNHFELFKLKKAGLTNLNINNIINYLKKNSLTSLSVRNMAVVSK
                     CKNPTFFIENYKQLDLKKLRQEFKKFPVLSILDSNYPLELKEIYNPPVLLFYQGNIEL
                     LSKPKLAVVGARQASQIGCQSVKKIIKETNNQFVIVSGLARGIDTAAHVSALKNGGSS
                     IAVIGSGLDVYYPTENKKLQEYMSYNHLVLSEYFTGEQPLKFHFPERNRIIAGLCQGI
                     VVAEAKMRSGSLITCERALEEGREVFAIPGNIIDGKSDGCHHLIQEGAKCIISGKDIL
                     SEYQ"
     gene            4917..7037
                     /gene="topA"
                     /locus_tag="ACUBV3_001635"
     CDS             4917..7037
                     /gene="topA"
                     /locus_tag="ACUBV3_001635"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HHU3848124.1"
                     /translation="MATTTKTSTKKTSKKKSATAKKNLVIVESPAKAKTIEKYLGRNY
                     KVVASVGHIRDLKKSSMSIDFENNYEPQYINIRGKGPLINDLKKEAKKAKKVYLASDP
                     DREGEAISWHLAHILDLDKEDRNRVVFNEITKDAVKNAFVEPRQINMDLVDAQQARRV
                     LDRIVGYSISPILWKKVKKGLSAGRVQSVALKLIIDRENEIKAFQPEEYWTIDGSFKK
                     GTRKFNATFYGLDGKKFKLSNNEDVKTVLKRIKTDEFLVEKVEKKERRRNAPLPYTTS
                     SLQQDAANKINFRTRKTMMIAQQLYEGLSLGTAGHQGLITYMRTDSTRISPLAQNEAT
                     EFITNRFGANYSKHGNKVKNASGAQDAHEAIRPSSVNHTPESIAKYLDKDQLKLYTLI
                     WNRFIASQMTAAVFDTMKVNLTQNGVTFIANGSQVKFDGYMAVYNDTDKNKMLPDMEE
                     GESVKKVNTNPEQHFTQPPARFSEASLIKTLEENGVGRPSTYAPTLETIQKRYYVKLA
                     AKRFEPTELGEIVNSLIVEFFPDIVDVTFTAEMEGKLDEVEIGKEQWQKIIDEFYKPF
                     EKELAKAETEMEKIQIKDEPAGFDCELCGSPMVIKLGRYGKFYACSNFPECHNTKAIT
                     KEIGVICPICQKGQVIERKTKRNRIFYGCDRYPECEFTSWDKPIGRTCPKSNDFLVEK
                     KVRGGGKQVVCSNEKCDYQEEKIK"
BASE COUNT         2363 a         1160 c         1251 g         2283 t
ORIGIN      
        1 tatgttatta tagatttgtg ctcatttttg aacactttat ctttcatttt aaggagtttt
       61 atgaaaaagc tattaaccac tcctgtaatg ggttgtactt tggttatact ttctctaacg
      121 tccctatttg ttggtgttaa atctatccca ctggagcaaa taacacacct taatcaatca
      181 caggtagata tttttttagc gagtcgctta ccaagaacca ttagtatcct aatatctggt
      241 gcaagtttgt cagtctgtgg cctgctaatg caacaactca cccaaaataa gttcgtttca
      301 ccgacaactt cagggactat ggattgggcg aaattaggag tagttgttac gctgattttc
      361 tttaaaaata cctctatttt tattcaacta tgtattgctt caggttttgc cattttagga
      421 tcgctgcttt ttgtaactat tttaaaaatg attactttta aggataatat ttttatacct
      481 cttatcggac tgatgcttgg acaaattgta gcagcagcaa cagtattttt aggaacacat
      541 tttcaagtac tacaatctgt caacagttgg cttcaaggta atttttccat catgactagc
      601 catcgctacg aaattcttta cttagcattg ccatgtttgt ttttagtgta tttttttgct
      661 catcagttta ccattgttgg gcttggagaa agcttcgcta aaaatttagg gattgcttat
      721 gaaaagatga tttactttgg tttagttttg gtttcaatta tgacatccct agtcattatt
      781 atcgtaggag ctttaccttt cttaggttta atcgtaccta atctaattag cttaactaaa
      841 ggggatcata tgtcctcaac tatcttagag acatcgcttt taggggcttg tatcgtcatg
      901 atatgtgatc tttttggtcg cttagttatt tttccttatg aagtctcaat aggagttacg
      961 ctaggagtat tgggtagtgc ctttttcttg atttcaatta taagaaatga gggaaaatca
     1021 tgattaagga tttagagata ccaaaattac ttattttact gatactacta attgctacca
     1081 ttattttgtt tttaatttat ggtattccaa ctgacgcaaa tgaatttttg attatctata
     1141 tcctaaaaac acgttatcaa aaattgattg cattaatttt ggttggtatc tgcattggta
     1201 gttctagtct tatttttcaa actctaacca ataaccgact tttaactccg tccattattg
     1261 gtttagatag cttatatgtt cttatccaaa caggattaat gtatttaatc ggagctcagc
     1321 gtgtcataaa attttcatct tttagcagct tcctcctttc attattatta atggttggat
     1381 tcgcttactt attatttacg atactttttc gaaataaaaa acagtctttg tattttgttc
     1441 tattagctgg actcatattt aatacattat tttcttctat ctcatccttt attcaagcca
     1501 ttatggaccc caacgatttt atgattttac aaaatcagct ctttgctagt ttcaatgcta
     1561 ttaataccaa gatcctttgg atttctttca ttatcattgt cgttagcttt gtcattaatt
     1621 ggccttttat aaaggaattg gatgtcttac tattgggtaa agaaaatgcg atatcactcg
     1681 gcatctcata tcaaaagctt actactagaa tttttttatg gttagcattg atggtagcta
     1741 tagcaacagc attagttggg ccgattactt ttttaggctt acttgtagca catattacct
     1801 atcacagttt tcataccttt agacatcaaa tattagttcc tatagccatt gttatctgta
     1861 tctttacact tgtcttaggg cagcatctcg ttcaaaatct attacacctc actgttcaac
     1921 taagtgttct cctcaacctt atcgggggaa gttattttat attcacctta atcaaaggaa
     1981 ggaaaaactt atgattcaaa taaacaattt acataagttc tatggccaaa aggaaatatt
     2041 aaaagacatt aatatttcga ttcctaaagg aaaagtaaca gctattttag gtccaaatgg
     2101 atctggaaag agtacattgc tgagttgtat cagtcgactt gaaccttatg ataatggtga
     2161 aattttcctt gataaagtac cattagccca ctacagttca aatgacttag ccaaaacatt
     2221 agccatatta agacaatcta atcacttaac actcaagata aaggtgagtg acttgatagg
     2281 attcggtaga tttccttata gtaaaggacg gttaagtcaa aaagataaag ctgtcataga
     2341 atcagttata tcttatatgg atttaaacga tatagctgac gagttcatta ataacctcag
     2401 cggaggtcaa attcaaagag cttttatcgc aatgacaatg gcacaagata ctcaatatat
     2461 ttgcttagat gagccactca acaatttaga tatgaaatat gctgttcaaa tgatggatct
     2521 tatcaaacga tatgcgtatg agtttaataa aactatagtg attattattc acgacatcaa
     2581 ttttgcaact cactacgctg acaatgttgt cgctttaaaa gaaggacaag tggtgtcttg
     2641 tgggactgtc gaagatgtta tgcaagaaaa aatcctttct catttatttg atatgcctat
     2701 ccgtatcgaa actgttgatg gtaaaccaat tatccttttt tgtacaacgt aaataaagag
     2761 atttatgtag gataacattt ttagttttta tctattttag gagattttat ttaatgacaa
     2821 aaaaacttat tattgctata ttagcactat gcactatctt aaccacttct caagctgttt
     2881 tagctaaaga aaaatcacaa actgttacca taaaaaacaa ctattcggtc tatattaaaa
     2941 aagaaaaaag agacaagccg gataataaaa agcaaatcag cgagacactt aaagttcctt
     3001 taaaacccaa aaaagtagtt gtttttgata tgggagcttt ggatactatc acagctttag
     3061 gagctgaaaa atctgttatt ggtatcccga aggctaaaaa tgctctaagt ttattgccca
     3121 ataacatcaa atctgtttat aaagctaaga gataccaaga cgtaggaagt ctcttcgaac
     3181 caaactttga agctattgct cgtatgcaac ctgatgtggt tttcctagga gcacgtatgg
     3241 cttctgttga taatattgaa aaattaaagg aggctgcacc taaagcagca ttagtatatg
     3301 ctggagtcga ctcaaaaaaa gtatttgaca aaggagttgc tgagcgtgtc acaatgttag
     3361 ggaaaatctt cgaccaaaat aaaaaggcaa aaacctttaa taaagatatc gcacaagctg
     3421 ttcttaaatt gcagaaaact attgagaaaa aaggtaaacc tacagctcta tttgtaatgg
     3481 caaacagcgg tgaactttta actcaatcac cttctggtcg ttttggttgg attttctctg
     3541 taggtggatt taaagcagtc aatgaaaatg aaaaactaag ttcacatggt actcccgtat
     3601 cttatgaata catcgctgaa aaaaatccta actatctctt tgttttagat cgtggagcga
     3661 ctattggaca aggagcttca tcaaaagaac tttttaataa cgatgttatt aaagcaactg
     3721 atgctgtcaa aaacaaacgt gttcatgagg tagatggaaa agattggtat atcaattcag
     3781 gcggaagccg agtaacactc cgtatgatta aagatgtaca gaactttgtt gataatcgtt
     3841 aattttttgt caaaggctag gaaaaatcct agtcttattt ttttgaaaaa atagctatta
     3901 tcaatgcttc agagtataga ttgatcttat cactttattt tgtctattat tttcgaatat
     3961 agtcaatgga ggtaacctta tgaatcattt tgaactattt aagcttaaaa aagctggact
     4021 aacaaatctt aatatcaaca atattatcaa ctatctcaaa aagaatagtt taacttctct
     4081 atctgttcgc aatatggccg tagtatcaaa atgtaaaaat cctactttct ttatagaaaa
     4141 ttataaacag ctagacctta aaaaacttcg acaagaattc aaaaaatttc cagttctatc
     4201 gattttggat tctaactatc ctttagagtt aaaagaaata tataatccac cagttctact
     4261 tttttatcag ggtaatattg aacttctatc taaacctaaa ttagctgtag tgggggcaag
     4321 acaggcatct cagataggtt gtcagtctgt taaaaagatt atcaaggaga ctaacaatca
     4381 atttgttatc gtaagtggtt tagcgcgtgg cattgataca gcagcacatg ttagtgcttt
     4441 aaaaaatggc ggcagtagta tagctgttat cgggagtggt ttagatgttt attatccaac
     4501 ggagaataag aaacttcaag aatatatgtc atataatcat ctcgtattat cagaatattt
     4561 taccggagaa caacccttga aatttcattt ccccgaacgt aaccgtatta ttgcagggct
     4621 gtgtcaaggt attgtggttg ccgaagctaa gatgagatct ggaagtttaa ttacctgtga
     4681 aagagcatta gaagagggac gagaagtttt tgccattccc ggaaatatta tcgatggcaa
     4741 atcagatgga tgccaccatc ttatccaaga aggagctaaa tgcattattt caggaaaaga
     4801 tatcctttct gaatatcagt agtgttttgc tttctactat aggagaagtt ttctattgac
     4861 agtttaagaa aagtgagtta ttatgctata agtttacgat atagaaagtg atacctatgg
     4921 caactacgac aaaaacgtcg accaaaaaaa caagtaaaaa aaaatcagct actgctaaaa
     4981 aaaatctagt tattgtggag tctcctgcaa aagcaaagac tatcgaaaaa tacttaggac
     5041 gtaactataa agttgtagcc tcagtaggtc atatccgtga tttaaaaaag tcaagtatgt
     5101 ccattgattt tgagaataat tacgaaccac aatatattaa tattcgtggt aaaggacctc
     5161 tcattaatga tttaaaaaaa gaagctaaaa aggctaaaaa agtttacctc gcgagtgacc
     5221 cggaccgtga aggagaagct atttcctggc atttagcgca tattttagat ttagacaaag
     5281 aagacagaaa ccgtgttgtt ttcaatgaaa tcacaaaaga tgctgttaaa aatgcttttg
     5341 tggaacctcg tcagattaac atggatctcg ttgatgctca gcaagcacga cgtgttttag
     5401 atcgcattgt tggttattct atctcaccta ttttatggaa aaaagtaaaa aaagggctat
     5461 cagctggacg tgtacaatca gttgctctta aactaatcat tgatcgtgaa aatgagatta
     5521 aggctttcca accggaggag tattggacta ttgacggttc ctttaaaaaa ggaacacgta
     5581 aattcaatgc cactttctac ggtttagacg gaaagaaatt caaattatcg aataatgaag
     5641 acgtcaaaac agttctaaaa cgtattaaaa ctgatgaatt cttagttgaa aaagttgaaa
     5701 aaaaagagcg tcgtcgtaat gcaccattac cgtatacaac ttcttcattg caacaagatg
     5761 cagctaataa aatcaatttt cgaactcgta aaaccatgat gattgcgcaa cagctgtacg
     5821 aaggacttag cttaggcaca gcaggtcatc aaggtctgat tacctatatg cgtactgatt
     5881 ctacacgtat tagtccgtta gcacaaaatg aagcaacaga atttattact aaccgttttg
     5941 gtgcaaatta ttctaaacat gggaataaag ttaaaaatgc ttctggagca caagacgctc
     6001 acgaagctat tcgcccatct agtgttaatc atacacccga aagtattgct aagtatttag
     6061 acaaagatca actaaaactt tacactctta tctggaaccg ttttattgca agccaaatga
     6121 cagcagcagt ctttgacaca atgaaagtta atttaacgca aaatggtgtt acttttattg
     6181 ctaatggtag ccaagtcaag tttgatggtt acatggctgt ttataacgat actgacaaaa
     6241 ataaaatgtt accagatatg gaggaaggag aaagtgttaa aaaggttaat acgaatcctg
     6301 aacaacactt cactcaacct cctgcaaggt tttcagaagc aagtctcatt aaaacacttg
     6361 aagaaaacgg tgtaggtcgt ccttcaactt atgccccaac gcttgagact attcaaaaac
     6421 gttattatgt taagcttgca gctaaacgct tcgaaccaac tgaacttggt gaaattgtta
     6481 atagccttat tgtagaattt ttcccagata ttgttgatgt taccttcact gccgaaatgg
     6541 aagggaaact agacgaagtt gagattggta aagagcagtg gcaaaagatt atcgatgaat
     6601 tttataaacc atttgaaaaa gaacttgcaa aagcagaaac tgaaatggag aaaattcaaa
     6661 taaaggatga acctgctgga tttgattgcg agctatgtgg atcaccaatg gtaataaaac
     6721 taggacgtta tggaaagttt tatgcgtgta gcaatttccc tgaatgtcat aacacaaaag
     6781 ctatcactaa ggaaataggt gttatttgtc ctatctgtca aaaaggacaa gttattgagc
     6841 gaaaaacaaa acgcaatcgt atcttttatg gctgtgaccg ctacccagaa tgtgagttta
     6901 catcttggga caaacctatt ggacgaactt gccccaaatc aaatgatttc ttagtggaga
     6961 aaaaagtacg cggtggtggt aaacaagttg tttgctcaaa tgaaaaatgc gactaccaag
     7021 aagagaaaat taaataaagt ggaatctgag tgtttag
//
LOCUS       DBJYSH010000096         6769 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.096, whole genome shotgun sequence.
ACCESSION   DBJYSH010000096 DBJYSH010000000
VERSION     DBJYSH010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 6769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6769
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.096"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            complement(<1..1217)
                     /locus_tag="ACUBV3_001636"
     CDS             complement(<1..1217)
                     /locus_tag="ACUBV3_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate MFS transporter"
                     /protein_id="HHU3848125.1"
                     /translation="MKNLNRYVVAVSGVVLHLMLGSTYAWSVFRNPIISETGWDISSV
                     SFAFSLAIFCLGMSAAFMGHLVERFGPRIMGMISAILYGAGNVLTGLAIETQQLWLLY
                     VAYGILGGIGLGSGYITPVSTIIKWFPDRRGLATGFAIMGFGFASLVTSPLAQSLLIR
                     IGVGKTFYILGLVYFFVMMIASQFIKQPPQEKITILTHDGKKNAMNSQIITGLKANVA
                     IKSKTFYIIWLTLFINISCGLGLISAASPMAQDLAGYSAESAALLVGVLGIFNGFGRL
                     LWASLSDYIGRPLTFIILFIVNFIMTSSLFLSFNAIVFAIAMSILMTCYGAGFSLLPA
                     YLSDIFGTKELATLHGYSLTAWAIAGLFGPLLLSKTYSWGNSYQLTLMVFGFLFLFGL
                     LLSLYLRKLTTKVV"
     gene            complement(1404..2771)
                     /locus_tag="ACUBV3_001637"
     CDS             complement(1404..2771)
                     /locus_tag="ACUBV3_001637"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HHU3848126.1"
                     /translation="MKLSLHEVAKVVGAKNQVSEFEDVPLGNIEFDSRNISEGDLFLP
                     LKGARDGHEFIEMAFDNGAIATISEKEIEGHPYLLVSDALKAFQVLAQYYIEKMNVDV
                     IAVTGSNGKTTTKDMIAAILSTTYKTYKTQGNYNNEIGLPYTVLHMPEDTEKIVLEMG
                     QDHLGDIHVLSEIAKPRIAVVTLIGEAHLEFFGSREKIAEGKMQITDGMSSDGILIAP
                     GDPIIDPYLPANQMTIRFGHDQELQVTELKEEKHSLTFKTNALEHQLRIPVPGKYNAT
                     NAMVAAYVGKLLAVAEEDIVDALENLQLTRNRTEWKKSANGADILSDVYNANPTAMRL
                     ILETFSAIPNNDGGKKIALLADMKELGEQSVDLHNQMIMSIRPDSIDTLICYGQDIEG
                     LAQLASQMFPIGKVYFFKKNQEVDQFDQLLAKVKDTLKEKDQILLKGSNSMNLSKIVD
                     ILEVG"
     gene            complement(2918..3964)
                     /locus_tag="ACUBV3_001638"
     CDS             complement(2918..3964)
                     /locus_tag="ACUBV3_001638"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HHU3848127.1"
                     /translation="MSKETLILLYGGRSAEREVSVLSAESVMRAINYDKFFVKTYFIT
                     QVGQFIKTQEFDEMPSSDEKLMTNQTVDLDKMVRPSDIYDDNAIVFPVLHGPMGEDGS
                     IQGFLEVLRMPYVGTNILSSSVAMDKITTKQVLATVGVPQVAYQTYFEGDDLEHAIKL
                     SLETLSFPIFVKPANMGSSVGISKATDESSLRSAIDLALKYDSRILIEQGVTAREIEV
                     GILGNNDVKTTFPGEVVKDVDFYDYDAKYIDNKITMDIPAKVDEATMEAMRQYASKAF
                     KAIGACGLSRCDFFLTKDGQIFLNELNTMPGFTQWSMYPLLWENMGLTYSDLIEKLVM
                     LAKEMFEKRESHLI"
     gene            complement(4105..4701)
                     /gene="recR"
                     /locus_tag="ACUBV3_001639"
     CDS             complement(4105..4701)
                     /gene="recR"
                     /locus_tag="ACUBV3_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HHU3848128.1"
                     /translation="MLYPTPIAKLIDSFSKLPGIGTKTATRLAFYTIGMSDEDVNEFA
                     KNLLAAKRELTYCSVCGNLTDDDPCLICTDKTRDQSVILVVEDSKDVSAMEKIQEYNG
                     LYHVLHGLISPMNGISPDDINLKSLITRLMDGQVTEVIVATNATADGEATSMYISRVL
                     KPAGIKVTRLARGLAVGSDIEYADEVTLLRAIENRTEL"
     gene            complement(4716..6761)
                     /gene="pbp2b"
                     /locus_tag="ACUBV3_001640"
     CDS             complement(4716..6761)
                     /gene="pbp2b"
                     /locus_tag="ACUBV3_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein PBP2B"
                     /protein_id="HHU3848129.1"
                     /translation="MLNRKKRYRLTVKKQNASIPRRLNLLFFIIVLLFTVLILRLEQM
                     QIGQQSFYMKKLTALTSYTVKESKARGQIFDAKGVVLVENDERPTVAFSRGNNISSQS
                     IKELANKLSHYITLTEVASSDRAKRDYYLADKANYKKVVESLPDSKRYDKFGNHLAES
                     TVYANAVAAVPVSAINYSEDELKVVALFNQMNATPTFGSVKLSTGELSDDQIKKLDAD
                     KKELLGISVTSNWHRRKKGTSLSDILGTISTEKAGLPREEVKKYLKKGYSLNDRVGTS
                     YLEKQYEDDLQGIRQIRKVVVNKKGKVVSDNITQEGKSGRNLKLTIDLNYQNKVESIL
                     KQYYGSELSSGRASFSEGMYAVAIEPSTGKVLAMAGLKNDHGNLVDDSLGTIAKNFTP
                     GSVVKGATLSSGWENKVLRGNEVLYDQEIANIRSWFTRGLTPISAAQALEYSSNTYMV
                     QVALRLMGQDYNTGDALTDRGYQEAMAKLRKTYGEYGLGVSTGLDLPESEGYVPGKYS
                     LGTTLMESFGQYDAYTPMQLGQYISTIANNGNRLAPHVVSDIYEGNDSNKFAQLVRSI
                     TPKTLNKIAISDQELAIIQEGFYNVVNSGSGYATGTSMRGNVTTISGKTGTAETFAKN
                     INGQTVSTYNLNAIAYDTNRKIAVAVMYPHVTTDTTKSHQLVARDMIDQYISQFTGQ"
BASE COUNT         2169 a         1373 c         1003 g         2224 t
ORIGIN      
        1 acaactttag ttgttaattt tcttaaatat agagataaca ataatccgaa taagaataaa
       61 aaaccaaaaa ccattaatgt caattgatag gaatttcccc atgaatatgt ctttgataac
      121 aataggggcc caaacagacc tgctattgcc catgctgtta aactataacc atgtaaagta
      181 gctaattcct ttgttccaaa aatatcactt agataagcag gtaataagga aaaacctgca
      241 ccataacaag tcattaaaat agacatcgct attgcaaata caatagcatt gaatgacaaa
      301 aataaactag aagtcataat aaagttcaca ataaataata ttataaaggt caacgggcgt
      361 ccaatgtagt cagagagact tgcccataac agacgtccaa aaccgttaaa tatccctagt
      421 acccctacta ataaggctgc agattctgcg gaatagcctg ctaaatcttg tgccattggt
      481 gaagctgctg atattaaacc taagccacac gaaatattaa taaacaaggt caaccaaatg
      541 atgtaaaagg tttttgattt tatagcgacg tttgctttta atccagtgat aatttgtgaa
      601 ttcatagcat tctttttacc atcgtgagtc aaaatagtta ttttttcctg aggtggttgt
      661 ttaataaatt gtgaggcaat catcatgaca aaaaaatata ctaatcccaa aatataaaac
      721 gttttaccca caccaatcct aatcagtaag gattgtgcaa gcggacttgt tactaaagaa
      781 gcaaagccaa atcccataat agcgaatcct gttgctagtc ccctcctatc aggaaaccat
      841 ttaataatag tcgatactgg agtaatataa cctgaaccaa gtccgattcc tcctaaaata
      901 ccgtatgcaa catacagtaa ccataactgc tgagtttcaa tggctaagcc tgttaacaca
      961 ttccctgctc catataaaat agcagaaatc attcccatta tcctaggacc aaaacgctct
     1021 actaagtgtc ccataaaagc tgcagacatt cctagacaaa aaatagccaa actaaaagcg
     1081 aatgaaactg atgaaatatc ccaaccagtc tctgagataa ttgggttacg aaacacactc
     1141 caagcataag ttgatcctag cattaaatgt aaaacgactc cagaaaccgc aactacataa
     1201 cgatttaaat ttttcaaata atcctcctaa aaacgaactt aagttttatt tttaaaaata
     1261 tagttcgcaa taaacagatt atcaaaaaac tgaatatatc accagttttc tgcttagcat
     1321 atctattttc ctcaataaaa ctgaatatta ttcgtgatta aaaaacaaaa aagttagggt
     1381 aacttgttac cctaacccct ctattatcct acttcaagaa tatcaactat tttagaaaga
     1441 ttcattgagt tagaaccttt taataatatt tgatctttct ccttaagagt atcttttact
     1501 tttgcaagca attgatcaaa ctgatccact tcttgattct ttttaaagaa atacacttta
     1561 ccaattggga acatctggct tgctaattga gctaacccct caatatcctg gccataacaa
     1621 atcaaagtat caatagagtc aggtctgata ctcataatca tttgattatg taaatccaca
     1681 gattgttctc ccagttcttt catatcagct aaaagagcaa tcttctttcc accatcatta
     1741 ttgggaatag ctgaaaaggt ttctagtatc aatcgcatag ccgttgggtt agcattgtaa
     1801 acatctgata aaatatctgc tccattggct gatttcttcc attcggtgcg attacgagtt
     1861 agttgaagat tttctagagc atccactata tcttcttctg ctactgctaa taattttcca
     1921 acataggcag cgaccatcgc attagttgca ttgtacttac caggtactgg tatacgtaat
     1981 tggtgctcca aagcattagt tttaaaagtt aaggaatgtt tttcttcttt gagttctgtc
     2041 acttgcaatt cttggtcatg gccaaaacga atagtcatct ggtttgctgg aagatatgga
     2101 tcaataattg gatctcccgg tgcaattaat atgccatctg aactcatacc atctgtgatt
     2161 tgcattttcc cttctgcaat cttttctcga ctaccaaaaa attctaagtg tgcttcacca
     2221 atcaaagtaa caacagcaat acgtggtttt gcaatttcag ataacacatg gatatctcca
     2281 agatgatcct gtcccatctc caaaacaatt ttttcagtat cttctggcat gtgtagcaca
     2341 gtatatggta agccaatctc gttattgtag tttccttgtg ttttataagt cttatatgtg
     2401 gttgataaga ttgcagctat catatcttta gttgttgtct taccatttga gccagttact
     2461 gcaataacat caacgttcat cttttcaata taatactgtg ccaagacttg aaatgcttta
     2521 agtgcatcag acactaaaag ataaggatga ccctctattt ccttctctga aatggttgct
     2581 attgctccat tatcaaaagc catctctata aactcatgtc catctcttgc tccttttaag
     2641 ggaagaaata agtcaccttc actgatatta cgactatcaa attcaatatt tcccagggga
     2701 acgtcttcaa actctgatac ttgattttta gcgccaacta ctttagcaac ttcatgcaaa
     2761 ctaagcttca tatcatttcc ttttctatta tttagcaaca atgttgtaga tagtatgcta
     2821 tcccttataa acgaatatac tcctaaatta taacacactt tcataacata tcatagattt
     2881 gaaaaagctc aactaagaaa gacttagttg aactttttta aattaaatga ctctcacgtt
     2941 tttcaaacat ttctttagct aacataacta acttttcaat caaatcacta taagttagcc
     3001 ccatattttc ccaaagcaga ggatacattg accactgagt aaaaccgggc attgtattca
     3061 gttcgtttaa gaagatttgt ccatctttcg tcaaaaagaa atcacagcgt gataaaccac
     3121 aagccccgat tgctttaaaa gctttacttg catattgacg cattgcttcc atagttgctt
     3181 catcaacttt agctggaata tccatagtaa ttttattatc aatatatttg gcgtcatagt
     3241 catagaaatc gacgtcttta acaacttcgc caggaaaagt tgtcttaaca tcattattgc
     3301 ctaaaatacc tacttcaatt tcacgagctg tcacgccttg ttcaatcaaa atacggctat
     3361 catacttgag agctaagtca attgcagagc gaagtgagga ttcatctgtc gcttttgaaa
     3421 tacctactga tgaccccata ttagccggtt ttacaaaaat tgggaaactt aaagtttcta
     3481 aagagagttt aatcgcatgt tccaaatcat caccctcaaa ataagtttga tatgcaacct
     3541 gaggtacacc tactgttgca aggacttgtt ttgttgtaat tttatccata gccacgcttg
     3601 aagatagaat attagtccca acataaggca tccttaaaac ttctaaaaat ccttggatag
     3661 aaccatcttc ccccattggt ccatgtaaaa cggggaaaac aattgcatta tcatcataga
     3721 tatcacttgg acgaaccatt ttgtctaaat caacagtttg gtttgtcatt aacttttcat
     3781 ctgaagatgg catttcatca aattcttgtg ttttaataaa ttgacctact tgcgtgataa
     3841 aataagtttt aacaaaaaat ttatcataat taatagcacg catgacgctt tctgcagaca
     3901 gtacagatac ttcacgttcc gcagagcgac caccgtaaag caagatcaat gtttcttttg
     3961 acatatttct tctcttttct atctaatcta agattgacct tttaataata ccatatttta
     4021 acttaacttt caaaaatcaa tccacttaaa gttagtattg taaaagaaaa agttaggaga
     4081 ttcccaacct tttctttata acaattataa ctctgtacga ttttcgattg ctcgaagcaa
     4141 ggttacttca tctgcatatt cgatatctga accaacagct aaacctctcg ctaatcgtgt
     4201 cactttgata ccagctggtt ttaagactcg tgaaatatac attgatgtag cttcaccatc
     4261 agcagtcgca ttggtagcaa caataacttc tgtcacctga ccatccatca atcgagtaat
     4321 taaacttttc aaattaatgt catcagggct aataccattc ataggtgaaa ttaagccatg
     4381 taagacatga tacaaaccat tatactcttg aattttttcc atagctgaca catctttact
     4441 atcttcaaca accaagataa cagactggtc acgcgtctta tctgtacaaa ttaagcaagg
     4501 atcatcatca gtcaaattac cacaaacaga acaataggtt aattcccgct tagcagctaa
     4561 taaattctta gcaaattcat taacatcttc atcactcata ccaatggtgt aaaaagctaa
     4621 acgtgttgct gttttagtac ctatacccgg taattttgaa aagctgtcaa tcaatttagc
     4681 aattggtgtt gggtaaagca ttcaaatgtc ctctcttatt gtcctgtgaa ctgtgaaata
     4741 tattgatcaa tcatatctcg tgcaactaat tgatgggatt ttgttgtatc agttgtaaca
     4801 tgcggataca ttaccgctac tgctatttta cgattagtat cgtaggcaat agcgtttaag
     4861 ttgtaggtag aaactgtttg tccatttata tttttagcaa atgtttcagc ggtaccagtt
     4921 ttaccactaa tggttgtcac attccccctc attgacgtac cagttgcata gccacttcca
     4981 ctattgacaa cgttataaaa accttcttga ataatggcta actcttgatc tgagatagct
     5041 atcttattta gtgttttagg agtgattgaa cgaaccaatt gagcgaactt attagaatca
     5101 ttcccttcat agatatctga aaccacgtga ggtgctaaac gattcccatt attcgcaata
     5161 gttgagatat actgaccaag ttgcattggt gtataggcat catactgacc gaacgattcc
     5221 attagagttg ttcctaagct gtattttcca ggtacataac cttctgattc aggtaaatct
     5281 aatcctgtag aaacccctaa accatattcg ccgtaagttt tccttagttt agccattgct
     5341 tcttgatagc ctcgatcagt taaagcatct cctgtattat agtcttgtcc cattaaacga
     5401 agtgctactt gaaccatata agtattagat gaatactcta gcgcttgtgc cgcagatatt
     5461 ggagtcaaac ctcgtgtaaa ccatgaccgt atattagcaa tttcttggtc ataaagcact
     5521 tcatttcctc taagaacttt attttcccat cctgatgata gcgtcgcacc tttcacaaca
     5581 gatcctggcg tgaaattctt agcaatagtg cctaggctgt catcaactaa attcccatga
     5641 tcatttttca gcccagccat tgctaacact ttaccagttg aaggttctat agcaactgca
     5701 tacatccctt cggaaaaact agcgcgccca ctagataatt cactgccata gtattgtttc
     5761 aagatacttt ctactttgtt ttggtaatta agatcaatcg ttaatttaag atttcttcct
     5821 gacttacctt cttgcgtaat attatctgaa acaactttac cttttttatt aacaactact
     5881 ttgcgaattt gtctgattcc ttgtaagtca tcttcatatt gtttttcaag gtaagaagtt
     5941 cccactcggt cattcagtga atagcctttt ttcaaatatt ttttaacttc ttctcttggt
     6001 aaacctgcct tttctgtaga aatagtacct aatatatccg ataaagaagt tcctttttta
     6061 cgacgatgcc aattacttgt tacagaaatt cctaacagtt ccttcttatc agcatccagt
     6121 ttcttgattt gatcatcact taattcacct gtacttaatt tcactgaacc aaacgttggt
     6181 gtcgcattca tttggttaaa taaggcaacc acttttaact catcttcaga atagttaata
     6241 gcgctcactg gaactgcagc aactgcattt gcatatacgg ttgattcggc tagatggtta
     6301 ccaaatttat cgtaacgttt agaatctggt aaactttcaa caactttttt gtagttggct
     6361 ttatctgcta aataataatc tctctttgct ctatcgcttg aagcaacttc ggttaaggta
     6421 atgtaatgtg ataatttatt cgctaactct ttgatagact gagatgaaat attgtttcct
     6481 ctagaaaacg ccacagtagg acgttcatca ttttcaacta agacaactcc cttagcatca
     6541 aaaatctgac ctctcgcttt tgattctttc actgtataac tcgtaagagc tgtcagtttt
     6601 ttcatgtaaa atgattgttg cccaatctgc atttgctcta aacgaagaat tagaacagta
     6661 aataataaaa caataataaa aaatagaaga tttaatcgtc taggtatgct agcattttgc
     6721 ttttttacag tgagacgata ccttttttta cgattcaaca ttaactcct
//
LOCUS       DBJYSH010000097         6760 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.097, whole genome shotgun sequence.
ACCESSION   DBJYSH010000097 DBJYSH010000000
VERSION     DBJYSH010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 6760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6760
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.097"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            2..499
                     /locus_tag="ACUBV3_001641"
     CDS             2..499
                     /locus_tag="ACUBV3_001641"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HHU3848130.1"
                     /translation="MITLKSQPQAYKSEVDSKLLEAFIKTKAYQNSCVIATYLSFDYE
                     YNTQLLIKQALCDGKRVLVPKTYPKGKMIFVDYQKDNLRATLFGLLEPVNDRAVEKAS
                     IDLIHVPGLIFNNKGFRIGYGAGYFDRYLSDFEGDTISTIYRCQRQDFVEEKHDVAVK
                     EVLCL"
     gene            490..1167
                     /locus_tag="ACUBV3_001642"
     CDS             490..1167
                     /locus_tag="ACUBV3_001642"
                     /EC_number="3.4.21.-"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HHU3848131.1"
                     /translation="MSMKKFAKEYPTTVLLVGLTTLVFLLMQLTYGSQAESSQVIFQF
                     GGIQGDYLKAYPTNLWRLISPIFVHIGWEHFLLNGLALYFVGQMGESIWGSLRFLILY
                     ILSGLMGNIFTLFFTPHVVAAGASTSLFGVFSAIAIAGYFGKNPYLKQVGKSYQVMIL
                     LNLFFNIFTPGVSLAGHVGGLVGGVLVAIFLTKQNGSLLFKTWQSILALMIFIIVSIS
                     LIGLSLV"
     gene            1299..2342
                     /locus_tag="ACUBV3_001643"
     CDS             1299..2342
                     /locus_tag="ACUBV3_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019315854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family lipoprotein"
                     /protein_id="HHU3848132.1"
                     /translation="MNRKIIGLTLLSLSVLTLTACGNRSDKSANKSDIKVAMVTNQGG
                     VDDKSFNQSAWEGLQKWGKKKGLTKGNGFDYFQSSNESDHANNLDTAASSGYNLIFGI
                     GFGLHDTIEKVSENNKDVKYVIVDDIIKGKENVASVTFADNEAAYLAGIAAAKTTKTK
                     TVGFIGGMEGVVVKRFEAGFKAGVKSIDPAIKVAVSYAGSFTDAAKGKTIAATQYATG
                     VDVIYQAAGGTGAGIFSEAKTENETRKESNKVWVIGVDRDQSQEGNYVSKDGKKANFV
                     LASTIKEVGKSLQSVAELTEKKQYPGGKVTVFGLKDSGVDIKEHQLSSEGSVAVKKAK
                     EDIVSGKIQVPMK"
     gene            complement(2433..3332)
                     /gene="galU"
                     /locus_tag="ACUBV3_001644"
     CDS             complement(2433..3332)
                     /gene="galU"
                     /locus_tag="ACUBV3_001644"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-alpha-D-glucose metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HHU3848133.1"
                     /translation="MKVRKAVIPAAGLGTRFLPATKALAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILVVTGKSKRSIEDHFDSNFELEYNLKEKGKNELLKLVDETTGIRLHFIRQ
                     SHPRGLGDAVLQAKAFVGNEPFVVMLGDDLMDITNNKVIPLTKQLINDFEATHASTIA
                     VMEVPHEDVSAYGVIAPQGEGVNGLYSVNTFVEKPSPEEAPSNLAIIGRYLLTPEIFN
                     ILETQKPGAGNEIQLTDAIDTLNKTQRVFARKFTGDRYDVGDKFGFMKTSIDYALQHP
                     QIKDDLKKYIIDLGKSLEKTSKK"
     gene            complement(3369..4385)
                     /locus_tag="ACUBV3_001645"
     CDS             complement(3369..4385)
                     /locus_tag="ACUBV3_001645"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HHU3848134.1"
                     /translation="MTKQKIAVLGPGSWGTALAQVLNDNGHEVRLWGNVVEQIEEINT
                     SHTNQRYFKDITLDSKIKAYTNLEEAINNVDSILFVVPTKVTRLVAKQVANLLKHKVV
                     IMHASKGLEPGTHERLSTILEEEISEQYRSDIVVVSGPSHAEEAIVRDITLITAASKD
                     IEAAKYVQKLFSNHYFRLYTNTDVVGVETAGALKNIIAVGAGALHGLGYGDNAKAAII
                     TRGLAEITRLGVQLGADPLTFSGLSGVGDLIVTGTSVHSRNWRAGDALGRGEKLEDIE
                     KNMGMVIEGISTTKVAYEIAQNLNVYMPITEAIYKSIYEGANIKDSILDMMSNEFRSE
                     NEWH"
     gene            4555..4884
                     /gene="rnpA"
                     /locus_tag="ACUBV3_001646"
     CDS             4555..4884
                     /gene="rnpA"
                     /locus_tag="ACUBV3_001646"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HHU3848135.1"
                     /translation="MKKTYRVKSDKDFQMIFSRGKNVANRKFVIYYLEKEQKHFRVGI
                     SVSKKLGNAVVRNAIKRKIRHVLLSQKTALQDYDFVVIARKGVEELDYQALEKNLIHV
                     LKIAGLI"
     gene            4897..5712
                     /locus_tag="ACUBV3_001647"
     CDS             4897..5712
                     /locus_tag="ACUBV3_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HHU3848136.1"
                     /translation="MKKKLKTFSLILLTGSLLVACGRGEVSSHSATLWEQIVYAFAKS
                     IQWLSFNHSIGLGIILFTLIIRAIMMPLYNMQMKSSQKMQEIQPRLKELQKKYPGKDP
                     DSRLKLNDEMQSMYKAEGVNPYASVLPLLIQLPVLWALFQALTRVSFLKVGTFLSLEL
                     SQPDPYYILPVLAALFTFLSTWLTNKAAVEKNIALTLMTYVMPFIILVTSFNFASGVV
                     LYWTVSNAFQVFQILLLNNPYKIIKVREEAVRVAHEKEQRVKRAKRKASKKRK"
     gene            5720..6541
                     /gene="jag"
                     /locus_tag="ACUBV3_001648"
     CDS             5720..6541
                     /gene="jag"
                     /locus_tag="ACUBV3_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HHU3848137.1"
                     /translation="MVLFTGATVEEAIEKGLQELNISRLRAHIKVVSREKKGFLGFGK
                     KPAKVEIEGITDEVTDINESVVLKNIKNVPSSVDVVEEYIEEVDETLEKEDVSQPELP
                     KIDDKNVVTTSEAIEKIDLLPNIEVAAAQVTKYVENIIYEMDLDATIETTTSKRQISL
                     QIETPEAGRIIGYHGKVLKSLQLLAQNYLHDRFSKSFSVSINVHDYVEHRTETLIDFS
                     KKIARRVLETNEPYHMDPMSNSERKTVHKTIATIEGVESYSEGNDPNRFVVVTKK"
BASE COUNT         2257 a         1055 c         1291 g         2157 t
ORIGIN      
        1 tttgataacc ttaaaatctc aacctcaagc ttacaaatca gaagttgact ctaaactttt
       61 agaggctttt ataaaaacta aagcttatca aaatagttgt gtaattgcta cttacctatc
      121 ttttgactac gaatacaata cacaacttct tattaaacaa gctttatgtg atggcaaacg
      181 tgtccttgtt cccaaaactt atccaaaagg taaaatgatt tttgtggact atcaaaaaga
      241 taacttgagg gcaacactgt ttggcttatt agaacctgtt aatgatagag ctgttgaaaa
      301 agctagtata gacttaattc atgtgcccgg acttatcttt aacaataaag gttttcgtat
      361 aggttatggt gcaggttatt ttgatcgcta tttgagtgat tttgaaggtg atactattag
      421 tactatttat aggtgtcaga gacaggattt tgtagaagag aagcatgatg tagcggttaa
      481 ggaggtatta tgtctatgaa gaagttcgct aaagagtatc cgacgacagt actcttggtc
      541 ggtctgacaa cccttgtttt tttattgatg caactaactt atggaagtca agcagagtct
      601 agccaagtta tttttcaatt tggtggaata caaggtgatt atctaaaagc atatccaaca
      661 aatctatgga gactaataag tcctatcttt gttcatattg gttgggaaca ttttttatta
      721 aatggtttag ctctatattt cgtaggacaa atgggggaaa gtatttgggg aagtttgaga
      781 tttctaattc tctatatttt atctggctta atgggtaata tttttacgct attttttact
      841 ccacacgtgg tggcagcagg agcctcaacg tctttatttg gtgttttttc tgctatagca
      901 atcgcaggtt attttggaaa aaatccttat ttaaaacaag ttggaaaatc atatcaagtg
      961 atgatcttgt taaatttatt ttttaatatc ttcacacccg gagtgagttt agcaggccat
     1021 gttggtggtt tagttggtgg agtattggtg gctatttttt tgacaaaaca aaacggttct
     1081 ttattattta aaacttggca aagtatttta gctttaatga tatttattat tgtatcgatc
     1141 agcttaatag gtctatcgtt ggtataattc tacggctata gtctctaaaa ccaaactttc
     1201 tctgggaaaa atagaataaa gtttgctaaa tgattttttt atgttatgat agaaatctgt
     1261 ttttatgtga ctataactta ttaggaggtt cattctcggt gaacagaaaa attattggct
     1321 taactctact atctttatct gtactaacat taacagcatg tggtaatcgt tcagataagt
     1381 ctgcaaataa atcagatatc aaagttgcga tggtaactaa tcaaggtgga gtagacgata
     1441 aatcgtttaa tcaatcagct tgggaagggc tacaaaagtg gggaaaaaag aaggggttga
     1501 caaaaggtaa tggctttgat tatttccagt catctaatga atcagatcat gctaataacc
     1561 ttgatacggc agcctctagt ggctataatt taatttttgg tattggattt ggtcttcatg
     1621 atactattga aaaagtatca gaaaataata aagatgttaa atacgttatt gtagatgata
     1681 tcattaaagg taaagaaaat gtagctagtg ttacttttgc agacaatgaa gctgcttact
     1741 tagctggaat tgctgcagct aagacaacta agacaaaaac agttggtttt atcggaggga
     1801 tggaaggcgt cgttgttaag cgatttgaag caggatttaa ggctggagta aaatcaattg
     1861 atccggctat taaagttgcg gtatcttatg caggctcatt tacagatgca gctaaaggta
     1921 aaacgattgc tgccacacag tatgcaacag gtgttgatgt tatttaccag gctgctggtg
     1981 gtacaggagc aggaattttt agtgaggcta aaactgaaaa tgagacccgc aaggaatcta
     2041 acaaagtctg ggtaattggt gttgacagag atcaaagtca agaaggcaat tatgtttcaa
     2101 aagatggtaa gaaagcaaac tttgttctgg catctactat aaaagaggtg gggaaatctc
     2161 tccaatctgt agcagaatta acagagaaaa aacaatatcc aggagggaaa gtaacagttt
     2221 ttggtctaaa ggatagtggt gtagatatta aagagcatca attgtctagt gagggatctg
     2281 ttgctgtcaa aaaagctaaa gaggatattg tttcagggaa aatacaagtt cctatgaaat
     2341 gatagaagtg tggaggaaca ataagttctt cgtcaagtac aataaaaaag tttcctattt
     2401 aggaaacttt tttattgtac ttgactcata ttttacttct tagatgtttt ttctagagat
     2461 tttcctaaat caatgatata tttcttcaag tcatctttaa tttgtgggtg ttgaagagca
     2521 taatcaatag atgttttcat aaaaccaaac ttgtcaccaa cgtcataacg gtcccctgta
     2581 aattttctag caaaaacacg ttgcgtttta tttaaagtat cgatagcatc ggttagttga
     2641 atctcatttc ctgcaccagg tttttgagtt tctaaaatat taaaaatttc tggagttaac
     2701 aagtagcgac caatgattgc caaattactt ggagcctctt cgggagaagg tttttcaacg
     2761 aaagtgttaa cgctatatag accattaact ccttctcctt gtggagcgat aacaccataa
     2821 gcagaaacat cttcatgtgg aacttccata acagcaattg ttgatgcatg agtagcttca
     2881 aagtcattaa ttaattgttt ggtgagagga ataactttat tattggtaat atccattaga
     2941 tcatcaccta gcataacaac aaaaggttca ttaccaacga aggcttttgc ttgtagcaca
     3001 gcatctccaa gcccacgagg atgactttgt cgaataaaat ggagtcgaat gccagttgtt
     3061 tcatcaacaa gtttgagaag ttcgtttttc cctttctctt ttaggttata ttctaattca
     3121 aaatttgaat caaagtggtc ttctatagaa cgttttgatt taccagttac aactaaaata
     3181 tcttcaattc cagattttag tgcttcttca acaataaatt gaatagttgg tttatcaacg
     3241 attggaagca tttctttagc taaagcttta gtagcaggta aaaaacgtgt tcctaaacca
     3301 gcggcaggga taactgcttt tctgactttc atatatagct cctttaaata gtattaatat
     3361 aaacgatatt agtgccattc attctctgaa cgaaattcat tggacatcat atctaaaatg
     3421 ctatctttga tattagcccc ttcatatatt gatttataaa tagcttctgt aatgggcatg
     3481 taaacattta agttttgggc gatttcgtaa gcaaccttag ttgttgagat accttcaata
     3541 accatcccca tatttttttc aatatcttca agtttctcac cacgtcctaa agcatctccc
     3601 gctctccagt tgcgagaatg aacagatgta ccagtcacaa ttaaatctcc aactccggat
     3661 aaaccactga aagtgagagg gtcggctcct aattgtacac caaggcgtgt tatctcagct
     3721 aaaccacgcg taataatagc tgctttagca ttatcaccat aaccaagacc gtgtagtgct
     3781 ccagcaccaa cagcaataat attttttaga gcaccagctg tttccactcc tacgacatca
     3841 gtgttagtat aaagtctaaa atagtgatta ctaaacaatt tttggacata cttagctgct
     3901 tcaatatctt ttgaagcagc agtaatcaaa gtaatatcac gaacgatggc ttcttcagcg
     3961 tgactgggac ctgaaacaac aacgatatca ctacgatatt gttcagagat ttcttcttct
     4021 aaaattgtag aaagtcgttc atgagtgcca ggttctagtc ccttagaagc atgcataata
     4081 acaactttat gctttaaaag attagccact tgtttagcaa caagtcttgt aactttagtt
     4141 gggacgacga atagaatgct gtcaacatta ttaatcgctt cctcaagatt agtgtaggct
     4201 tttattttgg aatctaaggt aatatctttg aagtaacgtt gattagtatg gctagtatta
     4261 atctcttcta tttgttcaac cacgttcccc cataagcgta cctcatgtcc attgtcattt
     4321 aatacttgtg caagagcagt cccccatgaa ccaggtccaa gaacggcaat tttttgtttt
     4381 gtcatcttat tccttctttc taataataag gcatatagcg ctaacattat atcatactct
     4441 aatacacttt gtcttgttta gtctgttgtc gatgatagta acattagaaa agaattgagt
     4501 cttttaggtt atttatggta taataaaacc aatttagata aatggagcac tattttgaaa
     4561 aaaacctatc gggtaaaaag tgataaggat tttcaaatga ttttcagcag aggtaaaaat
     4621 gttgctaatc gaaaatttgt tatctactat ttagaaaaag aacaaaaaca ctttcgtgtg
     4681 ggaatctctg ttagtaagaa attaggaaat gctgttgttc gtaacgctat taaaagaaaa
     4741 attaggcatg ttttactctc tcagaaaaca gcgctgcaag attatgattt tgtcgtgatt
     4801 gcgcgtaaag gtgtagagga attagactat caagcattag aaaaaaatct tatacacgtt
     4861 ttaaaaatag caggactcat ctagaaaggt ataaaattga agaaaaaact taaaacattt
     4921 agcttaatat tgctgacagg ttctttattg gttgcttgcg gacgaggaga agtatctagt
     4981 cattcagcca cattatggga gcaaatagtt tatgcatttg ctaaatcaat acaatggctc
     5041 tcctttaacc attcaattgg tttgggaata atcttattca ccctaataat cagagcgatt
     5101 atgatgccgc tttataatat gcaaatgaaa tccagccaaa agatgcagga aattcagcca
     5161 aggctcaagg aattacaaaa aaaatatcca gggaaagatc ctgatagccg tttaaagcta
     5221 aatgatgaaa tgcaatctat gtataaagct gagggagtta acccatatgc aagtgtatta
     5281 cctcttctaa ttcagttacc tgtcctatgg gctcttttcc aagctttgac tagggtttct
     5341 ttcttaaaag taggaacatt tttgagccta gaattatcac agccggaccc atattatatt
     5401 ttacctgtac tggcggcctt atttaccttt ttatcaactt ggttaactaa caaagcagct
     5461 gttgaaaaaa atattgcttt aactcttatg acttatgtta tgccgtttat catattggta
     5521 acaagtttta actttgctag tggtgttgtt ttatactgga cggtgtctaa tgcttttcaa
     5581 gtatttcaaa ttctattatt gaacaaccca tacaagatta taaaggttcg agaagaagca
     5641 gtaagagtgg cacacgaaaa agaacaaaga gttaaacgtg ctaagagaaa agcaagtaag
     5701 aaaagaaagt aggaaaaaca tggtattatt tacaggagcg actgtagaag aagctattga
     5761 aaaaggacta caagagttaa acatttcacg attgagggca catataaaag ttgtatcacg
     5821 tgagaaaaaa ggttttttgg gctttggtaa aaagccagca aaagttgaaa tagaaggtat
     5881 caccgacgaa gtaactgata ttaatgaatc ggtagtgtta aaaaatatta agaatgtacc
     5941 gtctagcgta gatgtggtgg aagaatatat agaggaggtt gatgaaacgc ttgaaaaaga
     6001 agacgtatca caaccagaac ttccaaaaat tgatgataaa aacgttgtaa ctacaagtga
     6061 agctattgaa aaaattgacc tacttcctaa tattgaagta gcagctgctc aggtgactaa
     6121 atacgttgaa aatatcatct atgagatgga tttagatgca acaatagaaa ctacgacaag
     6181 taagcgacaa ataagtctcc aaatagaaac gccagaagct gggcgcatca ttggttatca
     6241 tgggaaagta ttaaaatcgt tgcagttgtt agcacaaaat tatcttcatg accgcttttc
     6301 taagagtttt tcagtatcca ttaatgtcca tgactacgtt gagcatcgta cagaaacttt
     6361 aattgatttt tctaaaaaaa tagctagacg tgttttagag acaaatgaac cttaccatat
     6421 ggatcctatg agtaacagtg agcgaaaaac tgttcataaa acaattgcaa ctatagaggg
     6481 tgttgaaagt tattctgaag gtaacgatcc taatagattt gtagtagtta ctaagaagta
     6541 aaaagaggtt tcacctcttt ttttcttgcg cccgatctac attttcagaa aatgtagaaa
     6601 aagtatgaaa aaagagttga caaagtaaga gatgatgtag taaactaaat aagttgtctc
     6661 gagagagcag cttctagtaa cgcgtagtta acaacttaaa aaagtagttg acaaagcaga
     6721 ataagtttgc tagaatatag aagttgtctc aagagaggca
//
LOCUS       DBJYSH010000098         6633 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.098, whole genome shotgun sequence.
ACCESSION   DBJYSH010000098 DBJYSH010000000
VERSION     DBJYSH010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 6633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6633
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.098"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            complement(<1..590)
                     /locus_tag="ACUBV3_001649"
     CDS             complement(<1..590)
                     /locus_tag="ACUBV3_001649"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HHU3848138.1"
                     /translation="MANMYYTENPNVEHDIHELNVKLLGESFSFLTDAGVFSKRMIDY
                     GSQVLLNSLHFEKNKSLLDLGCGYGPLGISLAKVQGVKATMVDINTRALELAKKNATR
                     NGVVVEVFQSNIYENISKTFDYIISNPPIRAGKQVVHSIIEESICYLNTGGSLTIVIQ
                     KKQGAPSAKAKMLDTFGNCDILKKDKGYYILRSEKV"
     gene            696..1616
                     /gene="coaA"
                     /locus_tag="ACUBV3_001650"
     CDS             696..1616
                     /gene="coaA"
                     /locus_tag="ACUBV3_001650"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HHU3848139.1"
                     /translation="MNNEFINFDRISRENWKDLHQQSQALLTEKELESIKSLNDNINI
                     QDVIDIYLPLINLIQIYKRSQENLSFSKAIFLKKENYQRPFIIGISGSVAVGKSTTSR
                     LLQLLISRTFKDSHVELVTTDGFLYPNEKLIQNGILNRKGFPESYDMESLLNFLDTIK
                     NGITAKIPIYSHEIYDIVPNQLQTIETPDFLILEGINVFQNQQNHRLYMNDYFDFSIY
                     IDAENKQIEEWYLQRFNSLLQLAEADPSNYYHKFTQIPPHKAMELAKDIWKTINLVNL
                     EKYIEPTRNRADFIIHKGKHHKIDEIYLKK"
     gene            1670..1918
                     /gene="rpsT"
                     /locus_tag="ACUBV3_001651"
     CDS             1670..1918
                     /gene="rpsT"
                     /locus_tag="ACUBV3_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HHU3848140.1"
                     /translation="MEVKTLANIKSAIKRAELNVKQNEKNSAQKSAMRTAIKAFEANP
                     SEELYRAASSSIDKAASKGLIHTNKASRDKARLATKLG"
     gene            complement(2002..2835)
                     /locus_tag="ACUBV3_001652"
     CDS             complement(2002..2835)
                     /locus_tag="ACUBV3_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HHU3848141.1"
                     /translation="MLTHKNILLTIIFGLFMIILSACGMSNKEMAGIDNWEHYQKEKK
                     ITIGFDNTFVPMGFESRSGDYTGFDIDLANAVFKEYGISVKWQPINWDMKETELNNGN
                     IDLIWNGYSKTAERAKKVAFTNPYMNNHQVIVTKTSSHINSIKDMKGKKLGAQSGSSG
                     FDAFNAKPDILKKFVKGKEAVQYDTFTQALIDLKNNRIDGLLIDEVYANYYLKQEGNI
                     KAYYFVKTAYQGENFVVGARKVDRRLIEKINKAFKQLHNKGRFQKISYKWFGEDVYSK
                     E"
     gene            complement(2849..3478)
                     /locus_tag="ACUBV3_001653"
     CDS             complement(2849..3478)
                     /locus_tag="ACUBV3_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009854102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HHU3848142.1"
                     /translation="MLELKNISKCYGQKEIFKDFNLTVEEGKILSLVGPSGGGKTTLL
                     RMLAGLEKIDSGTIVHDGKEVSVDHLETLNLLGFVFQDFQLFPHLTVLDNLILSPVKT
                     MGLSKELAKEKALVLLERLGLKDHALVYPFSLSGGQKQRVALARAMMIDPQIIGYDEP
                     TSALDPELRQEVEKLILQNRETGMTQIVVTHDLQFAESISDTILKINPK"
     gene            complement(3488..4129)
                     /locus_tag="ACUBV3_001654"
     CDS             complement(3488..4129)
                     /locus_tag="ACUBV3_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HHU3848143.1"
                     /translation="MSYILEILPSLLVGAQVTLQVFFIVICLSIPLGIVVAFLMKVHF
                     KPLQWLLTTYIWIMRGTPLLLQLIFFYYFLPSVGISFDRMPAAIIAFTMNYAAYFAEI
                     FRGGIESIPKGQYEAAKVLKFSKFQTVWYIVLPQVFKIVLPSVFNETITLVKDSSLVY
                     ILGVGDLLLESKTAANRDATLAPMFIAGGIYLLLIGLLTILSKQVEKRFNYYK"
     gene            complement(4264..4593)
                     /locus_tag="ACUBV3_001655"
     CDS             complement(4264..4593)
                     /locus_tag="ACUBV3_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000056782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HHU3848144.1"
                     /translation="MSLPNCPKCNSEYVYEDGILLVCPECAYEWNPEEIEEEVGLIVL
                     DSNGTRLSDGDTVTVIKDLKVKGAPKDIKQGTRVKNIRLVDGDHNIDCKIDGFGAMKL
                     KSEFIKK"
     gene            complement(4756..6570)
                     /gene="glmS"
                     /locus_tag="ACUBV3_001656"
     CDS             complement(4756..6570)
                     /gene="glmS"
                     /locus_tag="ACUBV3_001656"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019804802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HHU3848145.1"
                     /translation="MCGIVGVVGNTNATDILIQGLEKLEYRGYDSAGIFVVGDNKSQL
                     VKSVGRIAELQAKVGDSVSGTTGIGHTRWATHGKPTEGNAHPHTSGSGRFVLVHNGVI
                     ENYLQIKETYLTKHNLKGETDTEIAIHLVEHFVEEDNLSVLEAFKKALHIIEGSYAFA
                     LIDSQDADTIYVAKNKSPLLIGLGNGYNMVCSDAMAMIRETSEYMEIHDKELVIVKKD
                     SVEVQDYDGNVIERGSYTAELDLSDIGKGTYPFYMLKEIDEQPTVMRKLISTYANESG
                     DMNVDSDIIKSVQEADRLYILAAGTSYHAGFAAKTMIEKLTDTPVELGVSSEWGYNMP
                     LLSKRPMFILLSQSGETADSRQVLVKANEMGIPSLTITNVPGSTLSREATYTMLIHAG
                     PEIAVASTKAYTAQVATLAFLAKAVGEANGKAEAKDFDLVHELSIVAQSIEATLSEKD
                     VISEKVEQLLISTRNAFYIGRGNDYYVTMEAALKLKEISYIQTEGFAAGELKHGTISL
                     IEDNTPVIALISADSTIAAHTRGNIQEVVSRGANALIIVEEGLEREGDDIIVNKVHPF
                     LSAISMVIPTQLIAYYASLQRGLDVDKPRNLAKAVTVE"
BASE COUNT         2215 a         1277 c          945 g         2196 t
ORIGIN      
        1 tagacttttt cacttctcaa tatataatat cccttatctt tttttaaaat atcgcaatta
       61 ccaaaagtat ctagcatttt agcttttgca cttggagcgc cttgtttctt ttgaatgaca
      121 atggtaaggc taccgcctgt attaaggtaa cagatacttt cttcgataat agaatgaaca
      181 acttgctttc cagcacgaat aggtgggttg cttataatat agtcgaaagt tttagaaata
      241 ttttcataaa tgtttgactg aaagacttca actactactc cattgcgtgt tgcatttttc
      301 ttagctaatt ccaatgctct cgtattaatg tcaaccatag ttgctttaac tccttgcact
      361 ttggctagag aaattcccag cggaccgtat ccacacccaa ggtctaacaa ggatttattt
      421 ttttcaaaat gtaaactatt tagaaggact tgactaccat aatctatcat tcttttagag
      481 aaaactcctg catcagttaa aaaactgaat gattctccta ataatttgac atttagctca
      541 tgaatatcat gttcaacatt aggattctct gtataataca tattagccat aaataattct
      601 aaccctccaa tttcgccata ttattttacc atattttgaa acggttttca atcactcaat
      661 aaaatgttat aatagctttc atataggagt ttgacatgaa taatgaattt attaatttcg
      721 accgtatttc cagagaaaat tggaaggact tacatcagca atcccaagct ttactaactg
      781 aaaaagagct tgaatctatc aaaagcttaa acgataatat taatattcaa gatgttattg
      841 acatctacct tcctttaatt aaccttattc aaatctataa acgtagtcaa gaaaatttat
      901 ctttttctaa agcaatattt cttaaaaagg aaaattatca acgtcctttt attatcggca
      961 tatcagggtc agttgctgtt ggaaaatcaa ctaccagtcg cttactacaa ttactaattt
     1021 cacgaacttt taaagatagt cacgtcgaat tagtcactac tgatggcttt ttatatccta
     1081 acgagaaact catacaaaat ggaattttaa accgtaaagg tttcccggaa tcctatgata
     1141 tggagtcact tctaaatttc ttagatacta taaagaacgg tataaccgcg aaaatcccaa
     1201 tttattctca tgaaatctac gatatcgttc caaatcaatt gcaaactatt gaaacaccag
     1261 attttttaat tttagaagga ataaatgtct ttcaaaacca acaaaaccat cgactttaca
     1321 tgaatgatta ttttgatttt tcaatataca ttgatgctga aaataaacaa attgaagaat
     1381 ggtatttaca acgatttaac agtcttctac aattagccga agcagaccct agtaattact
     1441 atcataaatt cacacaaata ccgccacata aagctatgga actagctaaa gatatttgga
     1501 aaactattaa tttagtcaac cttgaaaaat atatagaacc aaccagaaat agggctgatt
     1561 ttattattca taaagggaaa catcataaaa ttgatgaaat ttatctcaaa aagtgataaa
     1621 gggttgccaa aaatcactta attagatata cttatatagt tagattaata tggaggtgaa
     1681 aacattggca aatattaaat cagctatcaa acgcgctgaa cttaacgtta aacaaaacga
     1741 aaaaaactca gcacaaaaat cagctatgcg tactgctatt aaagcatttg aagctaaccc
     1801 ttcagaagaa ctttaccgcg ctgcttcttc aagcatcgac aaagctgctt ctaaaggttt
     1861 aattcacaca aacaaagcaa gccgcgataa agcacgtctt gcaactaaac ttggttaatt
     1921 aacctagaaa acatcggata tatcgatgtt ttttatttaa ttttttcgta gaataaaaga
     1981 ctaggagaaa ttcctagtct tttattcttt actataaaca tcttcaccaa accatttgta
     2041 agagattttt tgaaatctcc ccttattatg aagctgtttg aaagctttgt taatcttttc
     2101 aattagtcta cgatcaactt tacgagctcc tactacaaaa ttttctcctt gataagcagt
     2161 tttaacaaaa taataagctt ttatatttcc ttcttgcttt aaataatagt tagcataaac
     2221 ttcatcaatc aaaagaccat caatacggtt attttttaaa tcaatcaaag cctgagtgaa
     2281 agtatcgtat tgaactgctt cttttccttt tacaaacttt tttaaaatat caggtttagc
     2341 gttaaaagca tcaaaaccag atgaacccga ctgggctcct agttttttcc ccttcatatc
     2401 cttaatacta ttaatatgtg atgaagtttt agtaacaatt acttgatgat tattcatata
     2461 tgggtttgta aaagcgactt ttttagcacg ttctgccgtt tttgaataac cattccaaat
     2521 aaggtctata ttaccattat taagttcagt ttctttcata tcccagttaa taggctgcca
     2581 tttcactgaa ataccgtatt ctttaaaaac agcattagct aaatcaatat caaagccggt
     2641 atagtcacca gaacgacttt caaatcccat aggaacaaaa gtattatcaa atccaatagt
     2701 aattttcttt tccttttgat aatgttccca attatcaata ccagccattt ccttattaga
     2761 cataccacat gctgataata taatcataaa taatccaaat ataatggtta ataatatatt
     2821 tttgtgagtc aacaatctac ccccaagtct acttaggatt aattttgaga atcgtatcag
     2881 atatactttc agcaaattga agatcatgtg ttactacaat ttgtgtcata cctgtttctc
     2941 tattttgtaa aattagtttt tctacttctt gacgcaactc tggatcaaga gcgctagttg
     3001 gctcatcata accaataatc tgtggatcaa tcatcatcgc acgagctagt gcgacacgtt
     3061 gcttttgccc cccagataat gaaaatggat agactaaagc atggtcttta agtcccaagc
     3121 gttcaagtaa tactaaggct ttttctttag caagttcttt tgataatccc atcgttttaa
     3181 caggagagag gatcaaatta tctaatactg ttaaatgtgg gaataattga aagtcttgga
     3241 agacgaatcc taaaagattt agtgtttcca aatgatcaac agatacttct ttaccatcgt
     3301 gaacaatagt ccctgaatca atcttttcca atccagctaa catacgtaat agtgttgttt
     3361 ttccacctcc agaaggtcct actaaagaga gaattttacc ttcttctaca gtcagattga
     3421 aatctttaaa gatttctttt tgcccatagc atttagaaat attttttaat tctaacatcg
     3481 tccctcctta tttatagtag ttaaaacgct tttcaacttg tttagataaa atagttaaaa
     3541 gaccaattaa taataggtaa ataccacctg ctataaacat tggtgcaagt gttgcatccc
     3601 tatttgcggc tgtcttactt tctagaagta agtcgcccac accaagtatg taaaccaatg
     3661 aggaatcttt cactagagta atcgtttcat tgaaaacact cggtaagact attttgaaga
     3721 cttgaggtaa tactatgtac caaaccgttt gaaacttaga aaactttaat acctttgcag
     3781 cttcatattg ccctttagga atagactcaa tacctcctcg aaaaatctct gcaaaataag
     3841 ctgcataatt catcgtaaaa gcaataatcg cagctggcat acgatcaaaa gaaattccta
     3901 cacttggtaa aaagtaataa aaaaagataa gctgtaataa cagaggcgtg cctcgcataa
     3961 tccaaatata agttgtcagt agccattgaa gcggcttaaa atgaactttc attaggaagg
     4021 ctacaacaat accaagtgga attgataaac aaataacaat aaagaaaact tgcaatgtca
     4081 cttgcgcacc tactaataag ctaggtaaga tttctaaaat ataagacatg gtccccccta
     4141 aataaaaaat agatgttctc attatatcaa aattcaaacc aatgtcatag agctttttta
     4201 ataaaaatgc atgaaatcaa gtcatattca aaaagcaatt gaccgtcgcc aattgcctga
     4261 aagctatttt tttataaact cagattttag tttcatagct ccaaaaccat caattttaca
     4321 atcgatatta tgatcgccat caactaaacg aatattttta acacgagtgc cttgcttaat
     4381 atccttaggc gctcctttta cttttaaatc cttaataact gtcacagtat ctccatctga
     4441 taagcgcgtt ccattgctat ccaatacaat taaacctact tcttcttcga tttcttctgg
     4501 attccactcg taagcacatt ctggacaaac cagtaaaata ccgtcttcat agacatattc
     4561 agagttacac ttagggcaat ttggtaatga catttttctc tccttaaatt aattaataaa
     4621 atagtcagca agtaacattc taactgatta tagacttctt gacaatagga aaaagcctat
     4681 tttccttcaa aaataggctt ttttacaatt tttaatattt tgtagttaat attagcgtat
     4741 tttagtgtaa cgagtctatt caacagttac agcttttgcc aaattacgtg gtttatcaac
     4801 atctaagcca cgttgtaatg aagcatagta agcaattaat tgagtcggaa taaccattga
     4861 aatggctgat aagaatgggt gaactttatt aacaataata tcatcacctt cacgttccaa
     4921 cccttcttct acaataatca aagcatttgc tccgcgagaa acaacttctt ggatatttcc
     4981 acgagtatgc gctgcaatag ttgaatcagc agatattaaa gcaataacag gagtattgtc
     5041 ttcaatcaac gaaattgtac catgttttaa ttcacctgca gcaaaacctt ctgtctgaat
     5101 ataagaaatc tcttttagct tcagtgcagc ctccattgta acataatagt cgttaccacg
     5161 accaatgtag aaggcattac gtgtcgagat taacaactgc tctaccttct cagagataac
     5221 atctttttct gataaggtcg cttcaattga ttgtgctaca attgacaatt catgaaccaa
     5281 atcaaaatct tttgcttctg ccttaccatt tgcttctcct accgcttttg ctaagaaagc
     5341 taacgttgca acttgagcag tatatgcttt tgtagaagca acagcgattt caggtcctgc
     5401 atgaatgagc atcgtatagg ttgcttcacg agaaagtgtt gagccaggca catttgttat
     5461 agttaaacta gggattccca tttcgtttgc tttaactaaa acttgtctac tatctgctgt
     5521 ttctcccgat tgactaagta aaataaacat tggcctctta ctcaaaagtg gcatgttgta
     5581 accccattct gaagaaactc caagttcaac aggtgtatca gttaattttt cgatcatggt
     5641 cttagcagca aaaccagcat gatatgacgt tcctgcagcc aatatgtaaa gacgatctgc
     5701 ttcttggact gattttataa tatcagaatc cacattcata tctccagatt cgttcgcata
     5761 agttgaaatc aacttacgca tcacagttgg ttgctcatca atttctttaa gcatgtagaa
     5821 tggataagtc cccttaccaa tatctgataa gtcaagctct gctgtgtagc taccacgttc
     5881 aatgacatta ccatcataat cttgaacttc aacgctatct tttttaacaa taacgagttc
     5941 tttatcgtgg atttccatat attcacttgt ctcacgtatc atagccatgg catctgaaca
     6001 caccatatta taaccatttc ccaaacctat tagaagaggt gatttatttt tagctacgta
     6061 aatcgtatca gcatcttgag aatcaataag agcaaatgcg tatgatcctt ctataatatg
     6121 taaggctttt ttaaaggctt caagcactga taagttatct tcttcaacaa agtgttcaac
     6181 taaatgaata gctatttctg tatcagtttc acccttgagg ttatgttttg ttaagtaagt
     6241 ttctttaatt tgaaggtaat tttcaataac accattgtgt accaatacaa aacgaccaga
     6301 tcctgaagtg tgaggatgtg catttccttc agtaggttta ccatgggttg cccaacgtgt
     6361 atgaccgata cctgtcgtac cactaacaga atctcctact tttgcctgaa gctccgcgat
     6421 tcgaccgact gacttcacaa gttgactttt gttatcacct actacaaaaa tacctgctga
     6481 gtcatatcct cggtactcta gcttttctaa gccttgaatt aaaatatccg ttgcatttgt
     6541 atttccaact acacctacga taccacacat attattatta ccaataagag ctttctctta
     6601 tactttctat ctaaactttc tttttgaatt ggt
//
LOCUS       DBJYSH010000099         6572 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.099, whole genome shotgun sequence.
ACCESSION   DBJYSH010000099 DBJYSH010000000
VERSION     DBJYSH010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 6572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6572
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.099"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            63..1502
                     /locus_tag="ACUBV3_001657"
     CDS             63..1502
                     /locus_tag="ACUBV3_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HHU3848146.1"
                     /translation="MENFLAPLNWFSQNILQNPAFFVGLLVLIGYLLLKKPLHDVFAG
                     FIKATVGYLILNVGAGGLVNTFRPILVALAKKFNLEAAVIDPYFGLASANAKLETMGF
                     ISVATTALLIGFGINILLVALRKVTKVRTLFITGHIMVQQAATISVFVLLLIPQLRNG
                     FGAWAVGIICGLYWAVSSNMTVEATQRLTGGGGFAIGHQQQFAIWFVDKVAPFFGKKE
                     ENLDNLKLPTFLNIFHDTVVASATLMLVFFGGILAVLGPDIMSNVKLIGPGAFVPTKQ
                     AFFMYILQTSLTFSVYLFILMQGVRMFVTELTNAFQGISNKLLPGSFPAVDVAASYGF
                     GSSNAVLSGFAFGLIGQLITIALLVVFKNPILIITGFVPVFFDNAAIAVYADKRGGWK
                     AAVALSFISGIIQVALGAVAVGLLGLAGGYHGNIDFEFPWLAFGYIFKYLGIAGYVIV
                     CLFFLAIPQLQFMKSKDKEAYYRGDAPAE"
     gene            1530..1808
                     /locus_tag="ACUBV3_001658"
     CDS             1530..1808
                     /locus_tag="ACUBV3_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HHU3848147.1"
                     /translation="MVKVLTACGNGMGSSMVIKMKVENALRQLGVSNFESASCSVGEA
                     KGLAANYDIVVASNHLIHELDGRTKGHLVGLDNLMDDNEIKTKLQEIL"
     gene            1875..2360
                     /locus_tag="ACUBV3_001659"
     CDS             1875..2360
                     /locus_tag="ACUBV3_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002267837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HHU3848148.1"
                     /translation="MNLKQAFIENDSIRLKLSASDWKEAIKLSIDPLIESGAVDAEYY
                     DAIIESTEEFGPYYILMPGMAMPHARPEAGVKRDAFSLITLTEPVVFPDGKEVSVLLA
                     LAATSSAIHTSVAIPQIIALFELENSIQRLTECQEAKEVLVMVEESKNSPYLEGLDLE
                     S"
     gene            2473..3138
                     /locus_tag="ACUBV3_001660"
     CDS             2473..3138
                     /locus_tag="ACUBV3_001660"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HHU3848149.1"
                     /translation="MTKRLPNLQVALDHSDLQGAIKAAVSVGHEVDVIEAGTVCLLQV
                     GSELVEVLRSLFPDKIIVADTKCADAGGTVAKNNAVRGADWMTCICCATIPTMEAALK
                     AIKEERGDRGEIQIELYGDWTYEQAQQWLDAGISQAIYHQSRDALLAGETWGEKDLNK
                     VKKLIDMGFRVSVTGGLSTDTLQLFEGVDVFTFIAGRGITEADDPAAAARAFKDEIKR
                     IWG"
     gene            3142..4005
                     /locus_tag="ACUBV3_001661"
     CDS             3142..4005
                     /locus_tag="ACUBV3_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HHU3848150.1"
                     /translation="MTRPIGIYEKATPKHFNWLERLQFAKELGFDFVELSIDESDERL
                     ARLEWSKEERLELVKAIFETGVRVPTITFSGHRRFPMGSNNPEKEARAMDMMKKCIVF
                     AQDIGIRNIQLAGYDVYYEEKSPETRARFIKNLRQACTWAEEAQVILSIEIMDDPFMN
                     SIEKYLAVEKEIDSPYLFVYPDTGNVSAWHNDLWSEFYNGHRSIAALHIKDTYAVTET
                     SKGQFRDVPFGQGCVDWEEMFAVIKKTNYNGPFLIEMWSENCETVEETRAAIKEAQDF
                     LYPLMEKTGVK"
     gene            4007..4723
                     /locus_tag="ACUBV3_001662"
     CDS             4007..4723
                     /locus_tag="ACUBV3_001662"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001934215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HHU3848151.1"
                     /translation="MAKSLQEMRERVCEANKSLPVHSLVKFTWGNVSEVDREAGLIVI
                     KPSGVDYDQLTPENMVVTDLEGNIVEGDLNPSSDLPTHVQLYKAWPEVGGIVHTHSTE
                     AVGWAQAGRDIPFYGTTHADYFYGPVPCARSLSEDEVNTAYEKETGSVIIEEFERRDL
                     DPMAVPGIVVRNHGPFTWGKDPAQAVYHSVVLEEVAKMNRFTEQINPRVEPAPKYIMD
                     KHYLRKHGPNAYYGQKGQEH"
     gene            4734..5381
                     /gene="fsa"
                     /locus_tag="ACUBV3_001663"
     CDS             4734..5381
                     /gene="fsa"
                     /locus_tag="ACUBV3_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HHU3848152.1"
                     /translation="MKYFLDTADVSEIRRLNRLGIVDGVTTNPTIISREGRDFKEVIN
                     EICQIVDGPVSAEVTGLTCDEMVTEAREIAKWSPNVVVKIPMTEEGLAAVSQLSKEGI
                     KTNVTLIFTVAQGLSAMKAGATFISPFVGRLEDIGTDAYALIRDLRHIIDFYGFQSEI
                     IAASIRGLAHVEGVAKCGAHIATIPDKTFASLFTHPLTDKGIETFLKDWDSFKKK"
     gene            5545..6528
                     /locus_tag="ACUBV3_001664"
     CDS             5545..6528
                     /locus_tag="ACUBV3_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HHU3848153.1"
                     /translation="MFEKVTISDIANLVGVSKATVSYYLNGNYKKMSLQTKEKIRLAI
                     KETGYQPSKIAQSLVTKNTRTIGVVIADITNPFISSVMKGIHDTCQQFGYSVNFTNSD
                     NDIDIELENLNRLNQQNVSGIILDSVDPNHSFIETLSNDRLVMVDRQAKDIKVDTVAS
                     DNKESTQIFLEKMQEAGYHDIYFVTYPIEGISTRELRYEGFKEVVSSNPDKLIIITED
                     GSTQRILDIIEHSEQKPGFLMMNGPTLLNFMKKLNQSTVSYPEDYGLGSYEDLEWMQV
                     LTPNVSCIKQDSYGIGCLAAQCLIEKISQGNEPTTARLLEVKNQIVIRQSF"
BASE COUNT         2066 a         1081 c         1349 g         2076 t
ORIGIN      
        1 cttacaaaaa atataataaa gatacaaaaa aacattactt aaataaagag aggtctttag
       61 ttatggaaaa cttcctagct ccccttaatt ggttctcgca aaatatcttg cagaatccag
      121 ccttcttcgt aggtctattg gtactcattg ggtatctttt attgaagaaa ccacttcatg
      181 atgtctttgc tggttttatc aaagcgaccg ttggatattt gattttgaac gtaggtgcag
      241 gaggattggt taatacattc cgtccaatct tagttgctct tgctaaaaaa tttaatttag
      301 aagctgctgt tattgaccct tactttggtt tagcatcagc aaatgctaag ttagaaacaa
      361 tgggctttat cagtgttgca acaacagcac tcttgattgg ttttgggatt aacattctct
      421 tggttgctct tcgtaaagta actaaagtcc gtactctttt tattactggt catatcatgg
      481 tacaacaagc tgcaacaatt tcagtatttg tactgctctt gattccacaa cttcgtaatg
      541 gttttggtgc atgggctgtt ggtatcattt gtggtctata ttgggcggta agttctaata
      601 tgaccgttga agcaactcaa cgccttacag gtggtggcgg atttgccatc ggacaccaac
      661 aacaatttgc aatttggttt gttgataaag ttgcgccatt cttcggtaaa aaagaagaaa
      721 accttgataa cttaaaatta ccaacattct taaatatttt ccatgataca gttgttgcat
      781 cagcaacatt gatgctagtt ttctttggag gaatcttagc tgtccttggt ccagacatta
      841 tgtctaatgt taaattaatc ggacctggtg catttgtacc aactaagcaa gctttcttca
      901 tgtacatcct tcaaacatca ttgacattct cagtttacct tttcattttg atgcaaggtg
      961 tacgcatgtt cgttacagaa ttgacaaatg ccttccaagg tatctcaaac aaacttcttc
     1021 caggatcatt cccagcagtt gacgttgcag cttcatatgg ttttggttct tcaaatgcag
     1081 ttctttcagg atttgctttt ggtttgattg gtcaattaat tacaatcgct ctacttgttg
     1141 tcttcaaaaa tccaatcttg attatcacag gattcgttcc agtattcttt gataatgcgg
     1201 caatcgctgt ttatgcagat aaacgtggtg gctggaaagc agcagtagct ctatcattta
     1261 tctcaggtat tattcaggtg gcacttggtg ctgttgctgt aggtctttta ggacttgcag
     1321 gtggttatca tggaaatatc gactttgaat tcccatggtt agcatttggt tacatcttca
     1381 agtatttagg tatcgctggt tacgtgattg tatgtctctt cttcttagct attccacaac
     1441 ttcaatttat gaaatctaaa gataaagaag catactatcg tggtgatgcg cctgcagaat
     1501 aatagaaatt tataacggag gaaataaaaa tggttaaagt tcttacagca tgcggaaacg
     1561 gcatgggatc atcaatggtt atcaaaatga aagtcgaaaa tgcgcttcgt caattaggcg
     1621 ttagcaactt tgagtcagca tcttgctcag tcggagaagc aaaaggttta gctgcaaact
     1681 acgatatcgt ggttgcttca aaccacctta tccatgaatt agacggacgt acaaaaggtc
     1741 atttagtagg tcttgataac cttatggatg acaatgaaat aaaaacaaaa ctacaagaaa
     1801 ttttgtaagt taatagacaa agggagtgag acaaaagttt ctctcccata tctttagact
     1861 tttaaggaga aatcatgaat ttaaaacaag cctttatcga aaatgactca attcgtctta
     1921 aattatctgc aagcgattgg aaagaagcaa tcaaattatc aattgatcca ttgattgaaa
     1981 gtggtgctgt tgatgcagaa tattacgatg ccattattga atctacagag gaatttggtc
     2041 cgtactacat tcttatgccg ggtatggcga tgcctcatgc tcgtccagaa gcaggcgtaa
     2101 aacgtgatgc attttcatta attacattaa cagagccagt agtttttcca gatggcaaag
     2161 aagtctcagt attgttagca cttgctgcaa caagttcagc tattcatact tctgttgcta
     2221 tccctcaaat tattgccctt tttgaattgg aaaattcaat tcagcgcttg acggaatgcc
     2281 aagaagcaaa agaagtcctt gtaatggtag aggaatcaaa aaacagccca tatttagaag
     2341 gtttagattt agaaagctaa tcaataaact gatattaata gaaaggatag gacctataaa
     2401 aaatctctgt gataagaaac tctttgccac aaataaaatg agtattttta tcaggtcctt
     2461 agaataaaca acatgacaaa acgtttacca aatttacaag ttgcacttga ccactcagat
     2521 ttacaaggag ctatcaaagc tgccgtatca gtaggacatg aagttgatgt tattgaagct
     2581 ggaactgttt gccttcttca agtaggtagt gaattagttg aggtccttcg cagtctcttc
     2641 cctgacaaaa taattgttgc tgatacaaaa tgtgctgatg ctggtgggac tgttgctaaa
     2701 aataatgctg ttcgcggtgc agactggatg acttgtatct gttgtgctac tattccaaca
     2761 atggaagcag ccttaaaagc tatcaaagaa gaacgcggcg atcgtggaga aattcaaatc
     2821 gaactatatg gtgattggac ttatgagcaa gcgcaacaat ggttggatgc aggtatttca
     2881 caagccattt atcaccaatc tcgtgatgcg cttcttgctg gcgaaacatg gggagagaaa
     2941 gaccttaata aggttaaaaa attgattgat atgggcttcc gagtttctgt aactggtggt
     3001 ttatcaactg atactcttca attatttgaa ggtgttgatg tctttacttt tattgcgggt
     3061 cgtggtatta cagaggcaga tgatccagcc gcagcagcac gtgcatttaa ggatgaaatt
     3121 aaacgtattt gggggtaata tatgacacgt ccaataggaa tttacgagaa ggcaacacca
     3181 aaacatttta actggttaga acgcctacaa tttgctaaag aattgggatt tgattttgta
     3241 gaattatcca ttgatgagtc agatgaacgt ttggcacgtt tggagtggtc aaaagaagaa
     3301 cgacttgagt tggtcaaagc tatttttgag acaggagttc gtgttccaac aattacattt
     3361 agtggtcatc gtcgtttccc aatgggatct aataatcctg agaaagaagc gcgtgcgatg
     3421 gatatgatga aaaaatgtat cgtctttgct caagatattg gtattcgtaa tattcaatta
     3481 gcaggatatg atgtgtacta tgaagaaaaa tctccagaga cacgtgcacg ctttattaag
     3541 aatcttcgtc aagcttgtac ttgggccgaa gaagcacaag ttattctatc tattgagatt
     3601 atggatgatc catttatgaa ttccattgag aaatatttgg ctgtggaaaa agagattgat
     3661 tctccttatc tctttgtcta cccagatact ggaaatgtat cagcatggca taatgatctt
     3721 tggagtgaat tttataatgg acatcgctca attgcagcgc ttcatattaa agatacttat
     3781 gctgttaccg aaacgtctaa aggtcaattc cgtgatgttc catttggtca agggtgtgtt
     3841 gattgggaag aaatgtttgc tgtcattaag aaaacaaatt ataatggtcc attcttgatt
     3901 gaaatgtggt cggagaactg tgagactgta gaggaaacac gtgcagccat taaagaggcg
     3961 caagattttc tttacccatt aatggagaaa acaggagtga aataatatgg caaaatcatt
     4021 gcaagaaatg cgtgaacgtg tgtgtgaagc taataaatca ctaccagttc atagtttagt
     4081 taaatttact tggggtaacg tttctgaagt tgaccgcgaa gcaggattga ttgttatcaa
     4141 accttcagga gttgactatg accaattgac acctgaaaat atggttgtca cagacttaga
     4201 gggaaatata gtggaggggg atttgaatcc atcatctgat cttccaactc atgttcaact
     4261 ttataaagca tggccagaag tcggtggtat tgttcatact cattcaacag aagcagttgg
     4321 ttgggcacaa gcgggtcgcg atattccatt ttatggaaca acacatgcag attattttta
     4381 tgggccagta ccatgtgcta ggtcacttag tgaagatgaa gttaacactg cttatgaaaa
     4441 agaaacaggt agcgttatta tagaggaatt tgaacgtcgg gatttagatc caatggctgt
     4501 tcctggtatt gttgtacgta atcatggacc atttacttgg gggaaagatc cagcacaagc
     4561 agtttatcac tcagtcgttc ttgaagaagt tgctaagatg aatcgtttca ctgaacaaat
     4621 taatccacgt gttgaaccag ctccaaaata tattatggat aaacactatt tacgtaagca
     4681 tggaccaaat gcttattatg gtcaaaaagg acaagaacat taaggagaat aatatgaaat
     4741 actttttaga tactgccgat gtttctgaaa ttcgtcgtct taatcgttta ggaatcgttg
     4801 atggtgttac taccaatccg actattattt cacgtgaggg gcgtgacttt aaagaagtta
     4861 ttaacgaaat ttgccaaatc gttgatggcc cagttagtgc agaagtgaca ggtttaactt
     4921 gtgatgaaat ggttaccgaa gctagagaaa tcgctaagtg gtcaccaaat gttgttgtta
     4981 aaattccaat gactgaagaa ggtttagcag cagtttcaca attatcaaaa gaaggtataa
     5041 agacaaatgt gacattaata tttactgtgg ctcaaggtct ttcggcaatg aaagctggtg
     5101 caacatttat tagtcctttt gtgggtcgtc ttgaagatat tggaacagat gcttatgctt
     5161 tgattcgtga ccttcgtcac attattgatt tttatggttt ccaatctgag attattgctg
     5221 ctagcattcg tggattggcg catgtggagg gcgtcgctaa atgcggtgcg cacattgcca
     5281 ctattccaga taaaacattt gcatcactat ttactcaccc gcttactgat aaagggattg
     5341 aaactttctt aaaagattgg gatagtttca agaagaagta gtgttacgat aaagcaaact
     5401 cttaattata ctagtgcaag acaaactttg ataatgggtt tgtctttttt tataatcttc
     5461 tccaataaga acacttattt tcatttttct tattaaatga tataatacgg ataaattaag
     5521 tctaattaga ataaggaaat agagatgttt gagaaggtaa ccattagtga tattgcaaat
     5581 ttagtaggtg tgtcaaaagc tacagtttcc tattatctca atgggaacta taagaaaatg
     5641 tctctgcaaa cgaaagaaaa aatacgttta gctattaagg agactggtta tcaaccaagt
     5701 aagattgctc aaagtttagt tacaaaaaat acccgtacta ttggggtcgt tattgctgat
     5761 attactaatc cctttatttc gtctgtgatg aagggaatac atgatacttg tcaacaattt
     5821 ggttactcgg ttaactttac taactcggat aatgatattg acattgagtt ggaaaattta
     5881 aaccgtttaa atcagcaaaa cgtttcagga attattttag attctgtaga tcctaatcat
     5941 tcatttatag aaactttgag taatgatcgt cttgtcatgg ttgatagaca agctaaagat
     6001 attaaagtag atactgtagc atccgacaat aaagaatcga ctcaaatatt tcttgaaaaa
     6061 atgcaagaag ctgggtatca tgacatctat tttgtcactt atcccattga agggatttca
     6121 acccgtgagc ttcgttacga aggttttaaa gaagtggtca gttcaaatcc tgataagttg
     6181 attattatca ctgaagatgg tagtacacag agaattcttg atatcattga acattcagag
     6241 caaaaaccag ggttcttaat gatgaacggt ccaactcttt tgaattttat gaaaaaatta
     6301 aatcaatcaa cggtcagcta ccctgaggat tatggtcttg ggagttatga agatttagaa
     6361 tggatgcagg tgcttacacc aaacgtttcc tgtataaaac aggattcata tggcattggt
     6421 tgcctggctg cccaatgttt aattgaaaaa attagtcaag gaaacgaacc aactactgca
     6481 aggttactag aagtaaaaaa tcaaatcgtt attcgtcaat ctttttaaaa atgtttgcga
     6541 gagcaagcat ttttttataa aaaagcaaaa aa
//
LOCUS       DBJYSH010000100         6556 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.100, whole genome shotgun sequence.
ACCESSION   DBJYSH010000100 DBJYSH010000000
VERSION     DBJYSH010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 6556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6556
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.100"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            50..1155
                     /locus_tag="ACUBV3_001665"
     CDS             join(50..326,326..1155)
                     /locus_tag="ACUBV3_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612000.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISSag2 family transposase"
                     /protein_id="HHU3848154.1"
                     /translation="MSRKVRRHFTDDFKQQIVDLYNVGRKRSSLIKVYELTPSTFDKW
                     VRQAKTTGSFKSVDNLTDEQRELIELRKHNKELEMQLDILKQAAVIMAPKREIITANK
                     DKYSISKMCRWLNMPHSSYYYQAVESVSETEFEETIKRIFLDSESRYGSRKIKICLNN
                     EGITLSRRRIRRIMKRLNLVSVYQKATFKPHSRGKNEAPIPNHLDRQFKQERPLQALV
                     TDLTYVRVGNRWAYVCLIIDLYNREIIGLSLGWHKTAELVKQAIQSIPYALTKVKMFH
                     SDRGKEFDNQLIDEILEAFGITRSLSQAGCPYDNAVAESTYRAFKIEFVYQETFQLLE
                     ELALKTKDYVHWWNYHRIHGSLNYQTPMTKRLIA"
     gene            1376..2511
                     /locus_tag="ACUBV3_001666"
                     /pseudo
     CDS             1376..2511
                     /locus_tag="ACUBV3_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612030.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            2551..2790
                     /locus_tag="ACUBV3_001667"
                     /pseudo
     CDS             2551..2790
                     /locus_tag="ACUBV3_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612023.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            complement(2989..5457)
                     /locus_tag="ACUBV3_001668"
     CDS             complement(2989..5457)
                     /locus_tag="ACUBV3_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HHU3848155.1"
                     /translation="MKKTYGYIGSVAAILLATHIGSYQLGKHHMGLATKDNQIAYIDD
                     SKGKVKAPKTNKTMDQISAEEGISAEQIVVKITDQGYVTSHGDHYHFYNGKVPYDAII
                     SEELLMTDPNYHFKQSDVINEILDGYVIKVNGNYYVYLKPGSKRKNIRTKQQIAEQVA
                     KGTKEAKEKGLAQVAHLSKEEVAAVNEAKRQGRYTTDDGYIFSPTDIIDDLGDAYLVP
                     HGNHYHYIPKKDLSPSELAAAQAYWSQKQGRGARPSDYRPTPAPGRRKAPIPDVTPNP
                     GQGHQPDNGGYHPAPPRPNDASQNKHQRDEFKGKTFKELLDQLHRLDLKYRHVEEDGL
                     IFEPTQVIKSNAFGYVVPHGDHYHIIPRSQLSPLEMELADRYLAGQTDDNDSGSDHSK
                     PSDKEVTHTFLGHRIKAYGKGLDGKPYDTSDAYVFSKESIHSVDKSGVTAKHGDHFHY
                     IGFGELEQYELDEVANWVKAKGQADELVAALDQEQGKEKPLFDTKKVSRKVTKDGKVG
                     YIMPKDGKDYFYARYQLDLTQIAFAEQELMLKDKKHYRYDIVDTGIEPRLAVDVSSLP
                     MHAGNATYDTGSSFVIPHIDHIHVVPYSWLTRNQIATIKYVMQHPEVRPDVWSKPGHE
                     ESGSVIPNVTPLDKRAGMPNWQIIHSAEEVQKALAEGRFAAPDGYIFDPRDVLAKETF
                     VWKDGSFSIPRADGSSLRTINKSDLSQAEWQQAQELLAKKNAGDATDTDKPEEKQQAD
                     KSNENQQPSEASKEEKESDDFIDSLPDYGLDRATLEDHINQLAQKANIDPKYLIFQPE
                     GVQFYNKNGELVTYDIKTLQQINP"
     gene            complement(5470..6390)
                     /gene="lmb"
                     /locus_tag="ACUBV3_001669"
     CDS             complement(5470..6390)
                     /gene="lmb"
                     /locus_tag="ACUBV3_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein/laminin-binding adhesin Lmb"
                     /protein_id="HHU3848156.1"
                     /translation="MKKVFFLMAMVVSLVMIAGCDKSANPKQPTQGMSVVTSFYPMYA
                     MTKEVSGDLNDVRMIQSGAGIHSFEPSVNDVAAIYDADLFVYHSHTLEAWARDLDPNL
                     KKSKVNVFEASKPLTLDRVKGLEDMEVTQGIDPATLYDPHTWTDPVLAGEEAVNIAKE
                     LGHLDPKHKDSYTKKAKAFKKEAEQLTEEYTQKFKKVRSKTFVTQHTAFSYLAKRFGL
                     KQLGISGISPEQEPSPRQLKEIQDFVKEYNVKTIFAEDNVNPKIAHAIAKSTGAKVKT
                     LSPLEAAPSGNKTYLENLRANLEVLYQQLK"
BASE COUNT         1976 a         1306 c         1209 g         2065 t
ORIGIN      
        1 gacagaaatt ataaagtgtt ctacacttaa gaaaaactgg agaaaaacca tgtctagaaa
       61 agtacgtcgt cacttcaccg atgattttaa gcaacaaatt gttgaccttt acaatgttgg
      121 tagaaagcgt agcagtctca tcaaggtata tgagctaacc ccttccacct tcgataagtg
      181 ggttagacaa gccaaaacaa ctggttcatt caagtctgtt gataatctga cagatgaaca
      241 gcgggagcta attgaactca gaaaacacaa taaagaactc gaaatgcaat tagatatcct
      301 aaagcaagca gcggtgatta tggcacaaaa agggaaataa tcactgctaa taaggataaa
      361 tacagcattt caaagatgtg tcgctggctg aatatgccac actcaagtta ttactatcaa
      421 gccgtggagt cagtatctga aacggagttt gaagaaacta ttaaaagaat tttcctcgat
      481 agcgagtcca gatacggatc cagaaaaatc aaaatatgct tgaataacga aggtatcaca
      541 ctttcacgtc gtcggattcg acgcattatg aagcgactca atttggtttc tgtttatcag
      601 aaagccacct tcaaaccaca ttctagaggc aagaatgaag cccctattcc caaccactta
      661 gacaggcaat ttaagcaaga aagaccacta caggccttag tcactgactt aacctatgtt
      721 cgtgtaggca atcgttgggc ttatgtttgc ctcatcattg acctatacaa ccgtgaaatc
      781 atcggcctgt ctcttggttg gcacaagacc gctgaactcg ttaagcaagc catacaaagc
      841 atcccttacg ccctgaccaa agtcaagatg ttccattcag atcgtggcaa agagtttgat
      901 aatcagttaa ttgatgaaat attggaagcc tttggaatca cacgttcgct tagtcaggct
      961 ggttgtcctt atgacaatgc cgtagctgaa agtacgtatc gtgctttcaa aattgaattt
     1021 gtttatcaag aaacctttca attgctggaa gaactagctc ttaagactaa agactatgtt
     1081 cattggtgga attaccaccg cattcatggt agtctcaact accaaacacc aatgactaaa
     1141 agattaattg cctagaaaaa cactttataa atgttgtaca gaaaagtgtt gccttttcac
     1201 ttaataaaag aaagattgaa ggaacacttc tctttcattc cgaccaaggg tcacaattta
     1261 agtccgggga atttagaaaa ataattgatg acaacaatag gcactgaatc cctaaaatga
     1321 gacaaaagaa aaacactcct gttgaaattt agtaaactag aaacaggagt gttttattat
     1381 ggttaaaaaa gcatattcat gggaaactaa gctggcttgc atcgatatga agaaagcagg
     1441 caagtctaac agagttatta tggagacttt aggcatcaaa aataatagtc aaatctatac
     1501 ttggatgaaa tggtacgaaa acgaggagct ttatcgtttt catcaaggtg ttggcaagca
     1561 atatacctac ggtaaaggct tggaacatct gtctgaagtg gaacaattac aattacaggt
     1621 tgacctctta aaaaagtatc gaggcttgat aaggaaatcg ataaagtaag tcttatcaag
     1681 cttgtggaag actataaaaa caactacccg atagttttca ttctagactg ttttaaggtc
     1741 aagcggtcga cctattaccg ttggaaaaag gagtatgaga agccgcaaaa aaagatgacc
     1801 ttattgaatt aattgaacag ctctgtatgg agaatcactt tatttatggc tatcgtacca
     1861 ttacacgatt gctcaaaaaa atccacggat tgactgttaa tacaaagaaa gtctatcgta
     1921 tcatgaaaaa taatggctgg ctttgtcgga cacgtaccaa gaaagttccc aatttgggta
     1981 aagcttatta tttaacagat aataaattga gtcgggattt ccatgccgat aagcctaagg
     2041 aaaagctcgt aacagatatt acctacctat atttcggtaa ctgtaaattg tatctctcat
     2101 caattatgga tctctataac cgagaaatta tagcttacac tatctcagat tgtcaggaca
     2161 cggattttgt actagacacc cttaatcagt taaaattgcc taaataaagg agcaatctta
     2221 cacagtgacc aaggctcagt gtatacttct atggcttact accaggcttg cacggaaaaa
     2281 gacattatcc gctcaatgtc tcgaaaagga acacctgcag ataatgcctg tattgaatgg
     2341 tttcacaccg tcctaaagac tgaaaccttt tatttccata ataggagaaa atacaacaaa
     2401 gatagtataa caaatattgt taaaaattac attacatttt ataatgaaac tagaattcaa
     2461 cagagattaa acgaccagtc gcctgtacag tacagaaaac tgattgccta aaagtgtttt
     2521 tcttgcgtct cactattagg gtgcagtgcc aatatcatgc attctttttc taatcctgga
     2581 tatccttatg ataatgccgt aactgaagca tttttcaagt atttaaagca tagacaaatc
     2641 aaccgaaaac attatcaaaa tatcaaacag gttcaattag actgctttga atacattgag
     2701 aatttttata acaattacaa cccacatacg gctaatctag gactaacccc taatcagaaa
     2761 gaagaaaatt attttaactc aataaaataa cacagttttc tgtctactta tttgacatta
     2821 gtccaagaat cctcctcttt ttttactatt ctaacatgga taccatttct ttgcaaaaga
     2881 aaaccgctat tatccaactt ttatcactac tcgttacata gaatgacttc aaaatcaccg
     2941 taacttgctt tgacaaagca gtgctttaac aatgagatct tcttttggtt aagggtttat
     3001 ttgttgaagt gtcttgatat cataagttac caattcacca tttttattat aaaattggac
     3061 accttctggt tggaaaatga gatacttagg atcgatatta gctttttgtg ctaattgatt
     3121 gatatgatct tctagggttg ctctatctag accatagtct ggtaaactgt ctataaagtc
     3181 atctgattct ttttcttctt tactggcttc acttggctgt tggttttcat tgctcttatc
     3241 tgcctgttgc ttttcttcag gtttatccgt atcagtagca tcaccagcat ttttctttgc
     3301 caataactct tgagcttgtt gccactcagc ttgggataga tcggatttat taatggttct
     3361 caatgaactg ccatctgctc ttgggatgct aaaggagcca tctttccata caaaagtttc
     3421 ttttgccaaa acatctcgtg gatcgaaaat atagccgtct ggtgctgcaa aacgaccttc
     3481 tgctagggct ttttgaactt cttcagcaga atggataatt tgccagtttg gcataccagc
     3541 acgtttatca agaggcgtaa catttggaat gaccgaacct gactcttcat gccctggctt
     3601 agaccataca tccggacgaa cttcggggtg ttgcatcaca tacttgattg ttgcaatctg
     3661 attgcgcgtc aaccatgaat acggaacgac atggatatga tcaatatgtg ggataacaaa
     3721 cgaacttcca gtatcgtaag tagcattacc agcatgcatc ggcagacttg acacatctac
     3781 agcaagtcgt ggctcaatgc ctgtatcaac aatgtcataa cggtaatgct tcttatcttt
     3841 aagcattagt tcttgttcgg caaaggcaat ctgagtcaaa tcaagttgat aacgagcata
     3901 gaaatagtcc ttgccatctt ttggcataat atagcccact ttaccatctt ttgttacttt
     3961 gcgactcact tttttagtgt caaagagtgg tttttctttg ccttgttcct gatccaaagc
     4021 agcaacaagc tcatcagctt gaccttttgc tttcacccag ttagcgacct catccaactc
     4081 atattgttca agttctccaa atcctatata gtggaaatga tctccgtgtt tagctgtaac
     4141 tcctgattta tccactgaat gaatggattc tttactaaaa acataagcat cactcgtatc
     4201 atatggttta ccatctaagc cttttccgta agctttgatg cgatgaccaa gaaaggtatg
     4261 tgtcacttct ttatctgatg gttttgagtg atctgaacct gagtcgttgt catcagtttg
     4321 gccggctaag tatcgatctg ctaattccat ttcaagaggt gataactgac ttcttgggat
     4381 aatatgataa tgatctccat gaggcaccac atacccaaaa gcgtttgatt tgatcacttg
     4441 agtcggttca aaaatcaacc catcttcttc cacatgacgg tatttcaaat caagacggtg
     4501 tagttgatct aaaagttcct taaaggtttt tcctttaaac tcatctcttt ggtgtttgtt
     4561 ttgtgacgca tcatttggcc taggaggcgc tggatgataa ccaccgttat ctggctgatg
     4621 accttgtcca gggttaggcg tcacatcagg aattggggct ttcctacgac ctggggctgg
     4681 tgtcgggcgg taatcagacg gtctagcacc tcgaccttgt ttttgactcc agtaggcttg
     4741 tgcagcagct agctcacttg gagacaaatc ttttttagga atataatgat agtgattacc
     4801 atgaggtact aaataagcat ctcctaaatc atcaatgata tctgtcggac taaaaatata
     4861 gccatcgtct gtagtatagc gtccttgtct ttttgcttca ttgactgccg caacttcttc
     4921 tttactgaga tgggccactt gagctaaacc tttttcttta gcttctttag ttcctttggc
     4981 tacttgctca gcaatttgtt gtttggttcg aatgtttttg cgcttactac ctggcttgag
     5041 gtaaacataa tagttgccat tgactttaat aacgtaaccg tctaagattt cattgataac
     5101 gtctgattgt ttaaaatggt aattaggatc cgtcatcaac aactcttcac taataatcgc
     5161 atcataagga actttcccat tgtaaaaatg ataatggtca ccgtgtgagg taacataacc
     5221 ttggtcagta attttgacta cgatctgttc agcagagatg ccttcttcag cactgatttg
     5281 atccatcgtt ttgtttgttt taggggcttt taccttacct ttgctatcat caatataggc
     5341 aatctgattg tcctttgttg ctagacccat atgatgctta ccaagctggt aacttccaat
     5401 atgagtagct agtaaaatag cagcaactga gccgatataa ccatatgttt tcttcactaa
     5461 taatctcctt tacttcaact gttgatagag cacttccaaa tttgctctaa gattttctag
     5521 atatgtcttg tttccgcttg gagcagcttc aagtggactt aatgtcttta ctttagctcc
     5581 tgttgatttc gcaatagcat gagcaatttt ggggttgacg ttgtcttctg caaaaatagt
     5641 cttgacgttg tattctttaa caaagtcttg aatttctttc aattggcgag gagagggctc
     5701 ttgctctgga gaaatacccg agataccaag ttgtttcaag ccgaatcgtt tagccagata
     5761 agaaaatgcc gtgtgttgcg tcacaaatgt ttttgagcgc acctttttaa atttttgagt
     5821 gtattcttca gttagttgct ctgcttcttt tttgaaagcc ttagcctttt tagtgtaact
     5881 gtctttgtgt ttaggatcca aatgtcctag ctctttagcg atattaacag cttcctcacc
     5941 agctaaaacg ggatccgtcc aggtatgtgg gtcataaagt gtcgcagggt caatgccttg
     6001 tgtgacttcc atatcttcta gccctttgac tctatctagt gtcagaggtt ttgacgcttc
     6061 aaacacatta acctttgatt tttttaaatt agggtctaga tcccttgccc aagcttctaa
     6121 ggtatgtgaa tggtaaacaa acaaatccgc gtcataaata gctgccacat catttacaga
     6181 cggttcaaag gaatgaatgc ctgcacctga ttggatcatc ctcacatcat ttaggtctcc
     6241 agatacttct tttgtcatcg catacattgg gtaaaagctg gttacaactg acatgccttg
     6301 cgtaggctgt ttggggtttg ctgacttatc acaccctgct atcattacta aactcacaac
     6361 catagccatg agaaaaaaaa cttttttcat agtacctcct caattataat ttaaccagtt
     6421 attaaccagt taattttact cctttatcaa tgctttgtca atgacaacta ccatctttta
     6481 tcacatcaac tgaagggata agcaaaaggc aattgttgac agctatttgt cctaacttct
     6541 ttttgatgag gtagca
//
LOCUS       DBJYSH010000101         6474 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.101, whole genome shotgun sequence.
ACCESSION   DBJYSH010000101 DBJYSH010000000
VERSION     DBJYSH010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 6474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6474
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.101"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            complement(<1..341)
                     /locus_tag="ACUBV3_001670"
     CDS             complement(<1..341)
                     /locus_tag="ACUBV3_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HHU3848157.1"
                     /translation="MKLSYEDKLEIYELRKIGMSWSQISQRYDVRISNLKYMIKLMDR
                     YGVEIVEKGRNEYYPPELKQEMIDKVLIHGCSQLSVSLDYALSNCSILTNWLSQFKKN
                     GYTIVEKTRGRP"
     gene            complement(416..2197)
                     /gene="uvrC"
                     /locus_tag="ACUBV3_001671"
     CDS             complement(416..2197)
                     /gene="uvrC"
                     /locus_tag="ACUBV3_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HHU3848158.1"
                     /translation="MNELIKHKLELLPDSPGCYLHKDKNGTIIYVGKAKNLKNRVKSY
                     FHGSHNTKTELLVSEIEDFEYIVTTSNTEALLLEINLIQENMPKYNIRLKDDKSYPYI
                     KITNERYPRLMITRQVKKSDGTYFGPYPDSGAATEIKRLLDRLFPFKKCTNPANKVCF
                     YYHLGQCNAHTVCQTNKAYWDSLREDVKQFLNGKDNKIVNGLTEKMKSAAMTMEFERA
                     AEYRDLIEAISLLRTKQRVIHQDMKDRDVFGYFVDKGWMCVQVFFVRNGKLIQRDVNM
                     FPYYNEPEEDFLTYIGQFYQDTKHFLPKEVFIPQDIDAKSVETIVGCKIVKPQRGEKK
                     QLVNLAIKNARVSLQQKFDLLEKDIRKTHGAIENLGNLLNIPKPVRIEAFDNSNIQGT
                     SPVAAMVVFVNGNPSKKDYRKFKIKTVIGPDDYASMREVIHRRYSRVLKDGLTPPDLI
                     VIDGGQGQVNIARDVIENQLGLAIPIAGLQKNDKHQTHELLFGDPLEVVELPRNSEEF
                     FLLHRIQDEVHRFAITFHRQLRSKNSFSSKLDGITGLGPKRKQLLMKHFKSLPNIQKA
                     DIEDIIMCGIPRTVAESLRDSLNDPPK"
     gene            2356..3123
                     /locus_tag="ACUBV3_001672"
     CDS             2356..3123
                     /locus_tag="ACUBV3_001672"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HHU3848159.1"
                     /translation="MIDVENILEKMKPNQKINYDWVMQQMVKQWQASDIRPKILMHVC
                     CAPCSTYTLEYLSQWADVTIYFANSNIHPKDEYYRREYVTQKFVHDFNKNTGYSVQFL
                     SAPYEPNEFFKIVHGLEEEPEGGDRCKVCYDFRLDKTAEKAVELGFDYFGSALTISPH
                     KNSQTINTIGIDVQKIYDTQYLPSDLKKNKGYQRSVEMCKDYDIYRQCYCGCIFGAKD
                     QGINLLQIKKDAKAFVSDKDGKEEFPNIRFTFNGKSM"
     gene            complement(3182..4522)
                     /locus_tag="ACUBV3_001673"
     CDS             complement(3182..4522)
                     /locus_tag="ACUBV3_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HHU3848160.1"
                     /translation="MYQTQTNKEKFVLFLKLFIPVLIYQFANFSATFIDSVMTGQYSQ
                     LHLAGVSTASNLWTPFFALLVGMISALVPVVGQHLGRGNKEQIRTEFHQFLYLGLILS
                     LILFLIMQFIAQPVLGSLGLEDEVLAVGRGYLNYMLIGIMPLVLFSICRSFFDALGLT
                     RLSMYLMLLILPFNSFFNYMLIYGKFGMPRLGGAGAGLGTSLTYWAIFIVIIIVMSLH
                     PQIKTYHIWTLERIKAPLIIEDIRLGLPIGLQIFAEVAIFAVVGLFMAKFSSIIIAAH
                     QAAMNFSSLMYAFPLSISTALAITISFEVGAERFQDANTYSRIGRLTAVGITSGTLLF
                     LFLFRENVAAMYNSDPHFVAITAQFLTYSLFFQFADAYAAPVQGILRGYKDTTKPFMI
                     GAGSYWLCALPLAVILEKNSQLGPFAYWIGLITGIFVCGLFLNQRLQKIKKLYY"
     gene            complement(4622..5038)
                     /locus_tag="ACUBV3_001674"
     CDS             complement(4622..5038)
                     /locus_tag="ACUBV3_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HHU3848161.1"
                     /translation="MVMKAIEIYMNINGRGAEAITFYEEAFNTKAEHVTTFAQSWPDC
                     PPELEHLIMNAQFNIDGIRMQLSDNNPIFDFQIGQNMTAALIVESVEEAQELFNKLSR
                     DAMNIKMPLQAVPWSPAYGNVTDKYGMTWQINTELS"
     gene            complement(5042..5539)
                     /locus_tag="ACUBV3_001675"
     CDS             complement(5042..5539)
                     /locus_tag="ACUBV3_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HHU3848162.1"
                     /translation="MIIDFKTVSTKGLETSPVAEALAGLRANEARYFWNKFKVAYETF
                     PVSDYPELMADINHLLKNERNLVFAAKPLEISQLFLENRILWTHVFYEDGLAINILYE
                     LDNPKKRAVGLKLSDGMDIPIELADKFKFARQKSKLAGIIRGSYFTLKGENETLMQRW
                     LDNMK"
     gene            5761..6357
                     /locus_tag="ACUBV3_001676"
     CDS             5761..6357
                     /locus_tag="ACUBV3_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ltp family lipoprotein"
                     /protein_id="HHU3848163.1"
                     /translation="MTKKIFLFICATLLVGIALYTPTTSIFNHNNVYAATKKAKIKFN
                     KTQKKIVKKAREYAKSGHMSKDSIIEKLKKDSKKYRQEDINFVINNLKVDYKKNALIS
                     AKIYSKTMNLSKQSIFEQLYSESPDKATHSDKFTKEESQYAIDHLKVDFKENALETAK
                     SYQSSSSLSKEEIYKQLTSTLGDKFTNDEAQYAVDHLK"
BASE COUNT         2220 a         1226 c          963 g         2065 t
ORIGIN      
        1 ggtctccctc ttgttttctc aacaatagta taaccatttt tcttaaattg ggaaagccaa
       61 tttgtgagta tagaacaatt agagagagca taatcaagag aaacagagag ttgagaacaa
      121 ccatgaatca agactttatc tatcatttcc tgctttaatt caggtggata atactcattt
      181 ctaccttttt cgacgatttc tacaccatac cgatccatga gttttatcat gtatttaaga
      241 ttcgagatac gaacatcata tcgttgacta atctgagacc aggacatccc gatctttctt
      301 aactcgtata tttctagttt atcttcataa cttaatttca tcagaaaagc accccaatcg
      361 ttagattttt tgtctaactt ttggggtgca gttcaatact gttctactaa gttttttatt
      421 taggaggatc attaagagaa tctcggagac tttctgcaac tgtcctagga ataccacaca
      481 taataatgtc ctcaatatca gctttttgga tattaggtaa gcttttaaaa tgtttcatta
      541 gcaattgttt acgttttggt cccaacccag tgataccatc taattttgaa gaaaaggaat
      601 ttttggagcg taattgacga tgaaaagtaa tagcaaaacg atgtacctca tcttgaatac
      661 ggtgaagtaa gaaaaattct tctgaattac gcggtaattc tactacttct aaaggatctc
      721 caaaaagtaa ttcatgtgtt tggtgtttat cattcttttg caaacctgca ataggtatgg
      781 ctaaacctaa ttggttctca ataacatcac gcgctatatt tacttgcccc tgaccaccat
      841 caattacaat caagtccgga ggtgtcaagc catcttttaa aaccctacta taacgccgat
      901 gaatgacttc tctcatacta gcataatcat ctggtccgat aacagtctta attttaaact
      961 ttcgatagtc ttttttactt ggatttccat tcacaaatac caccatagca gctactggac
     1021 ttgtcccttg aatattggaa ttatcaaatg cttctatgcg tactggttta gggatattaa
     1081 gaagattacc taaattttca attgccccat gagttttacg aatatctttt tccagtaagt
     1141 caaacttctg ttgtaaactg acacgagcat tttttattgc cagatttact aactgtttct
     1201 tttcaccacg ttgtggttta acaatcttac agcctacaat tgtttcaacc gacttagcat
     1261 caatatcctg tggaataaag acttctttgg gtagaaaatg ttttgtatct tgataaaatt
     1321 gccctatgta agttagaaaa tcttcctctg gttcattata ataaggaaac atattcacgt
     1381 cacgctgaat caattttcca tttcgaacaa aaaagacctg tacacacatc caacccttat
     1441 caacgaaata accaaaaaca tctctatcct tcatatcttg atgaataaca cgctgcttag
     1501 tacgcaacaa actaattgct tcgatcaaat ctcgatattc agccgcacgc tcaaattcca
     1561 tagtcatagc agcgctcttc attttttcag ttaatccgtt aacaatttta ttatctttac
     1621 cattaagaaa ctgttttaca tcttctctca gactatccca ataagcttta ttagtttgac
     1681 aaactgtatg tgcattgcat tgccctaaat gataataaaa gcatacttta tttgctggat
     1741 tagtacattt cttaaaagga aagagcctat ctagtaaccg cttaatctca gttgctgccc
     1801 cagaatccgg ataaggtcca aaataagtgc catctgattt ttttacttga cgtgtaatca
     1861 taagacgagg ataacgttca tttgttattt tgatatatgg gtaagactta tcatctttca
     1921 atcgaatatt atacttcggc atattttctt ggattaaatt aatttccaat aatagagctt
     1981 cggtattaga tgttgtcaca atgtattcaa agtcttcaat ctcagagacc aaaagttctg
     2041 tcttagtatt atgactccca tgaaaataac ttttcacacg attcttaaga tttttagcct
     2101 tacctacata aataattgtc ccattcttat ctttatgaag gtaacaacca ggactgtctg
     2161 gtaacaattc tagcttatgt ttaatcagtt cattcataga actattataa caaaatgtac
     2221 tgtaatcttt acgaaataaa tactattccc caacatctat tgtatcttcc aatacttgaa
     2281 taaaacccca atactcctag tctaagcaat tattttatgt tacactttaa acaagtgaat
     2341 tattgaggag tttttatgat agacgttgag aatattcttg aaaaaatgaa acctaaccaa
     2401 aaaatcaatt acgattgggt catgcaacaa atggttaagc agtggcaagc tagtgatatc
     2461 cgtccgaaaa ttctgatgca tgtttgctgt gctccttgta gtacatatac ccttgaatac
     2521 ttatcacaat gggcagatgt taccatctat tttgctaatt caaatatcca tcctaaagat
     2581 gaatattatc gccgcgaata tgttactcaa aaatttgttc atgatttcaa caaaaatact
     2641 ggatattctg tacaattctt atcagcacca tatgaaccaa atgaattttt caaaattgtt
     2701 catgggttag aagaagaacc tgaaggtggg gatcgttgta aagtttgcta tgatttccgt
     2761 cttgataaga cagcggaaaa agccgttgaa ctaggttttg attactttgg cagtgcccta
     2821 actattagtc ctcacaaaaa ctctcaaaca atcaatacta ttggtattga tgtccaaaaa
     2881 atttatgata ctcagtatct tcctagcgac cttaaaaaaa ataaaggtta ccaacgttcc
     2941 gttgagatgt gtaaagatta tgatatctac cgacaatgtt actgtgggtg tatcttcggt
     3001 gctaaagatc aagggataaa tcttctccaa atcaaaaaag atgcgaaagc tttcgtgagt
     3061 gataaagacg gcaaagaaga atttcctaat atacgcttca catttaatgg caagtcaatg
     3121 taaatgataa aaaaagactt agtaaagaac taagcctttt tggtataaat cttgtcaaac
     3181 tttaataata caacttctta atcttttgca gacgttggtt tagaaataga ccacaaacaa
     3241 aaatacctgt gattaaacca atccaatagg caaacggacc taactggcta tttttttcta
     3301 agataaccgc taatggcaaa gcacataacc aataagagcc cgcaccgatc ataaatggtt
     3361 ttgttgtatc cttatagcct cgtaaaatcc cctgtacagg agctgcataa gcatctgcaa
     3421 actggaaaaa gagactataa gttaggaatt gagctgtaat agcgacaaag tgagggtcac
     3481 tattatacat tgctgctaca ttctcacgaa atagaaataa aaatagtaag gttcctgatg
     3541 taatccctac cgctgttaag cgtcctatcc tactataagt gtttgcgtcc tgaaagcgct
     3601 ctgcccctac ttcaaacgat attgtaatag ctagagcagt ggaaatactt aaaggaaatg
     3661 catacattaa tgatgaaaaa ttcatagcag cctgatgtgc tgcaataatg attgaagaaa
     3721 attttgccat gaataagcct actactgcaa aaattgcaac ttctgcaaaa atttgtaaac
     3781 caatcggtaa tcccaatcga atatcttcaa taatcaaagg agcttttatt ctttccagag
     3841 tccatatatg atatgtttta atttgaggat gaagtgacat cacaataata ataacaataa
     3901 aaatagccca ataagttaaa gaagttccaa gacctgcccc cgcacctcct agtctaggca
     3961 taccaaattt accgtagata agcatataat taaaaaatga attaaagggt agaattaaaa
     4021 gcatcagata catagataac cttgttaacc ccaatgcatc aaagaatgaa cggcaaatgc
     4081 taaacaacac cagcggcatg attccaatca acatataatt taaataacca cgaccaactg
     4141 ctagaacttc atcttctaaa cccaaactcc ccaagacagg ttgagcaata aattgcatga
     4201 ttaaaaataa tattaaggac agtatcaaac ctaaatatag aaattgatga aattctgtgc
     4261 gaatttgttc tttatttcct ctacccaaat gttgaccaac tactggtact aatgctgaaa
     4321 tcatacctac taataaagcg aaaaacggag tccataaatt actagcagtt gacacacctg
     4381 ccaaatgtag ctgactatac tgtccagtca taaccgaatc aataaaagta gctgaaaaat
     4441 tagcaaattg ataaatcaat actgggataa ataatttcaa aaataaaaca aatttttcct
     4501 tatttgtctg agtttgatac atcttttttc tccttttcac cttaatcaat ttattttagc
     4561 ttagctagga tactctgtca atgctattta aaaaggttga gaaataatcc tcaaccccat
     4621 tttagctaag ctcagtattg atttgccatg tcataccata tttgtcagtc acatttccat
     4681 aagctggact ccacggaact gcttgtagag gcattttaat gttcattgca tctcgagaaa
     4741 gcttattgaa taactcttgc gcttcttcta cagattccac aattaaagct gctgtcatat
     4801 tttgtccaat ttgaaaatca aaaataggat tattgtcaga aagttgcatt cgaataccat
     4861 caatattaaa ttgagcgttc ataattaaat gttcaagttc tggcggacaa tctggccaac
     4921 tctgtgcaaa agttgtgacg tgttctgctt ttgtattaaa agcctcttca taaaatgtaa
     4981 tagcttcggc cccacgacca ttaatattca tataaatttc aattgctttc attaccatac
     5041 ctcacttcat attatccaac caacgctgca tcagagtttc attttcccct tttagggtaa
     5101 aataagaacc acgtattata cctgcgagtt ttgacttttg acgagcaaac ttaaacttgt
     5161 ccgctaattc aataggaata tccattccat ctgataattt taaacctaca gctctctttt
     5221 taggattatc taattcatat aaaatattaa tggctaaacc atcctcatag aaaacatgtg
     5281 tccataaaat acgattctct aaaaaaagct gtgaaatctc taaaggcttt gcagcaaaaa
     5341 caaggttacg ttcatttttt agaaggtgat taatatctgc catcaattct ggatagtctg
     5401 atactggaaa tgtttcgtag gctactttga atttattcca gaaatagcga gcttcatttg
     5461 cacgcaaacc agccaaggct tctgccactg gagaagtctc caaaccttta gttgaaacgg
     5521 ttttaaagtc gataatcatg ttctttcctt tttgattaaa atcttcgata atttagtata
     5581 ctcttttttg aagaaaaagt acattctaat cacttataaa aagtagtaca tttagaacac
     5641 taccacctca gtaatttctt gacctttctc tacttaatac ttataattaa tctagactta
     5701 atgtcaaagc atcaatcaag gtgtaaacct gattcaccta aaaagctgat ggagctaaac
     5761 atgaccaaaa agatattctt atttatctgc gctactcttt tagtaggaat agccttatat
     5821 acaccaacaa cttctatttt taatcataac aacgtttatg ctgccaccaa gaaagcaaaa
     5881 attaaattta ataaaactca aaaaaaaatt gtcaaaaaag caagagaata tgctaagtct
     5941 ggccatatgt ctaaagatag tatcattgaa aaattaaaaa aggattctaa gaaatatcgt
     6001 caagaagaca tcaactttgt gattaataac ctcaaggttg attacaaaaa aaatgccttg
     6061 atttctgcca aaatttattc aaagaccatg aatttatcta aacaatctat ctttgaacag
     6121 ctctattcgg agtctccaga taaggcaacc cactctgata aatttacaaa agaagaatcc
     6181 caatatgcta tagaccatct caaagtagat ttcaaagaaa atgctcttga gactgctaaa
     6241 agttatcaaa gtagttcttc tttatccaaa gaagaaatct ataaacaatt gacatctacg
     6301 ctgggcgata aattcacaaa tgatgaagcc caatatgctg tagatcattt gaaatgatga
     6361 cataccaata gtctatcctt atgtaataga attgatagct tatgctaata ttccagctgt
     6421 aaaatagcaa gaaccctcct aagttcatta agacttagga gggtttttat caat
//
LOCUS       DBJYSH010000102         6419 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.102, whole genome shotgun sequence.
ACCESSION   DBJYSH010000102 DBJYSH010000000
VERSION     DBJYSH010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 6419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6419
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.102"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            79..1713
                     /locus_tag="ACUBV3_001677"
     CDS             79..1713
                     /locus_tag="ACUBV3_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative polysaccharide biosynthesis protein"
                     /protein_id="HHU3848164.1"
                     /translation="MSQKTTKVSQQEQMVKGTAWLTAGNFISRLLGAIYIIPWYVWMG
                     KHAAEANALFGMGYEIYALFLLISTVGIPVAVAKQVSKYNTLGKEEMSIYLVRKILQF
                     MLILGGVFALIMYIGSPLFASLSKGGQELVPILRSLTLAVLVFPSMSVLRGFFQGFNN
                     LKPYAISQVAEQIIRVIWMLLTAFYIMRLGSGDYIAAVTQSTFAAFVGMFASIAVLLY
                     FLWRYNMLSALISKTPEHIKLDTKEILIETVKEAIPFIITGAAIQIFKLIDQFSFGNT
                     MALFTNYSSEELRVMFAYFSSNPGKVTMILIAVATAIAGVGIPLLTENFVKNDKKAAA
                     RLVVNNLQMLLMFLLPAVAGSVILAKPLYTVFYGLPQGQALGLFVISLIQTIILSIYT
                     VLAPMLQALFENRKAIIYFLYGLVAKVILQVPSIFLFHAYGPLFSTTVALCIPVILMY
                     LKIHEITGFKRQAIRRTSALVLILTLLMSFIISMIIWLMNLVIVPDSRLVSLVYIIVI
                     GAIGLGVYGFMALATHLLDKMIGSRAQDLRRKLHLN"
     gene            1848..3068
                     /gene="pepT"
                     /locus_tag="ACUBV3_001678"
     CDS             1848..3068
                     /gene="pepT"
                     /locus_tag="ACUBV3_001678"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HHU3848165.1"
                     /translation="MSYEKLLERFLTYVKINTRSNPNSTQTPTTQSQVDFALTVLKPE
                     MEAIGLKDVHYLPSNGYLVGTLPATSDRLRHKIGFISHMDTADFNAENITPQIVDYKG
                     GDIELGDSGYILSPKDFPNLNNYHGQTLITTDGKTLLGADDKSGIAEIMTAMEYLASH
                     PEIEHCEIRVGFGPDEEIGIGADKFDVKDFDVDFAYTVDGGPLGELQYETFSAAGLEL
                     TFEGRNVHPGTAKNQMINALQLAIDFHSQLPENERPEQTDGYQGFYHLYDLSGTVDQA
                     KSSYIIRDFEEVNFLKRKQLAQAIADNMNEALQSERVKVKLYDQYYNMKKVIEKDMTP
                     INIAKEVMEELDIKPIIEPIRGGTDGSKISFMGIPTPNLFAGGENMHGRFEFVSLQTM
                     EKAVDVILGIVAKD"
     gene            3097..3588
                     /locus_tag="ACUBV3_001679"
     CDS             3097..3588
                     /locus_tag="ACUBV3_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HHU3848166.1"
                     /translation="MIKLFGKIRYHWQPELSWAIIYWSIAIAPIFIGLSLLYERTEIP
                     SQVFVLFATFIVLVGIGFHRYFVIEEDGYLRIVSFNFLRRSKFPIEDIAKIEVTKSSV
                     TIKFNNNHERIFYMRKWPKKYFLDALAIEPKFKGEVELLDNLIKMDYFECYRYDKKAL
                     TKS"
     gene            complement(3575..3772)
                     /locus_tag="ACUBV3_001680"
     CDS             complement(3575..3772)
                     /locus_tag="ACUBV3_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HHU3848167.1"
                     /translation="MKVALIPEKCIACGLCQTYSNIFDYQDDGIVKFSDTDNLEKEIP
                     SSDQDTVLAVKSCPTKALTIL"
     gene            3818..4342
                     /locus_tag="ACUBV3_001681"
     CDS             3818..4342
                     /locus_tag="ACUBV3_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001085531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1386/EF1546 family surface-associated
                     protein"
                     /protein_id="HHU3848168.1"
                     /translation="MAKKPWEKKVVENNSHRKDKITRTSRGVVSSTPWITAFLSAFFV
                     IVVAILFIVFYTSNRGEDRAKETSGFYGASSQKVNSSKTKKASTSKKTTSSSKTKTSD
                     KSTTSSSTVASSSETSTSSSVNGGTIVVQPGEGAASIAARAGISVARLQELNPGHMTS
                     GYWYANPGDPVVVN"
     gene            4353..5036
                     /gene="cmk"
                     /locus_tag="ACUBV3_001682"
     CDS             4353..5036
                     /gene="cmk"
                     /locus_tag="ACUBV3_001682"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HHU3848169.1"
                     /translation="MNSINIAIDGPASSGKSTVAKIIAKNLNYTYLDTGAMYRCATYL
                     ALQHGYEAQDVSKILGLLAERPISFGKAEDGSQTVFLGTEEVTLAIRQNDVTNNVSWV
                     SAIPEIREELVNQQRRIAKDGAIIMDGRDIGTVVLPDAELKIFLVASVDERAERRFKE
                     NQEKGIESDFETLKSEIAARDYKDSHREVSPLKAAEDAIEFDTTGVSIEGVVTFIQEK
                     AEKIIDMKN"
     misc_feature    5057..5182
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            5197..5727
                     /gene="infC"
                     /locus_tag="ACUBV3_001683"
     CDS             5197..5727
                     /gene="infC"
                     /locus_tag="ACUBV3_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011226065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HHU3848170.1"
                     /translation="MKIIAKKDLFINDEIRVREVRLVGLEGEQLGIKPLSEAQAIADD
                     ANVDLVLIQPQATPPVAKIMDYGKFKFEYQKKQKEQRKKQSVVTVKEVRLSPVIDKGD
                     FETKLRNGRKFLEKGNKVKVSIRFKGRMITHKEIGAKVLAEFAEATQDIAIIEQRAKM
                     DGRQMFMQLAPIPDKK"
     gene            5767..5967
                     /gene="rpmI"
                     /locus_tag="ACUBV3_001684"
     CDS             5767..5967
                     /gene="rpmI"
                     /locus_tag="ACUBV3_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HHU3848171.1"
                     /translation="MPKQKTHRASAKRFKRTGSGGLKRFHAFTSHRFHGKTKKQRRHL
                     RKATMVSSGDFKRIKAMLTRLK"
     gene            6025..6384
                     /gene="rplT"
                     /locus_tag="ACUBV3_001685"
     CDS             6025..6384
                     /gene="rplT"
                     /locus_tag="ACUBV3_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019786714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HHU3848172.1"
                     /translation="MARVKGGVVSRKRRKRVLKLAKGYYGAKHILFRTAKEQVMNSYY
                     YAYRDRRQKKRDFRKLWITRINAAARMNGLSYSQLMHGLKLAEIEVNRKMLADLAVND
                     AAAFTALADAAKAKLGK"
BASE COUNT         2093 a         1007 c         1269 g         2050 t
ORIGIN      
        1 aaaagtttat aataaagtga tatttctgag atttttgtct tttaaatgct aaataatatt
       61 gaaaatagaa agtttaccat gtcacaaaaa acaacaaaag ttagtcagca agaacaaatg
      121 gtcaaaggga cggcttggtt aacagcaggg aatttcatta gtcgcttgct gggagccatt
      181 tatattatcc cttggtatgt ttggatggga aagcatgctg ccgaagcaaa tgctttattt
      241 gggatgggat atgaaatcta tgctttattt cttttgatat caactgttgg aattccagta
      301 gcagttgcaa aacaagtatc taagtataac actttaggta aagaagagat gagcatttat
      361 ctggttcgga aaatcttgca gtttatgctg attttaggtg gagtttttgc attaattatg
      421 tatattggtt cacctctatt tgcttcactt agtaaaggag ggcaagaact cgttccaata
      481 ttgcgtagtc taactctagc tgttttagtt tttccttcaa tgagtgtttt acgtggtttt
      541 tttcaaggtt ttaataattt aaaaccgtat gccattagtc aagtcgctga gcagattata
      601 cgtgtcatat ggatgttatt aactgctttc tatattatgc gtctaggatc aggagattat
      661 attgctgctg tgacacagtc aacattcgcc gcctttgtcg gtatgtttgc tagtatagct
      721 gtgctgcttt atttcttatg gcgttataat atgctatctg ccttgattag taaaactcca
      781 gagcacatta aattagatac taaagaaatc ctaattgaga ctgttaagga agcaatccct
      841 tttattatta ctggtgcagc aattcaaatt ttcaaattga ttgatcagtt ttcatttggg
      901 aatacgatgg cgctctttac gaattattca agtgaagaat tacgggttat gtttgcttac
      961 ttttcaagca accctggtaa agttacgatg attctaattg ctgttgctac cgcaattgct
     1021 ggagttggta ttccattgtt aacagagaat tttgttaaga atgataaaaa agccgccgct
     1081 cgtttggttg ttaataattt acaaatgtta ctaatgtttt tactgccagc tgttgcagga
     1141 tccgttattt tagcaaaacc tttgtataca gtcttttatg ggttaccaca gggacaagct
     1201 ttagggcttt ttgtcatatc tcttattcaa acaattattt tatctattta tactgtttta
     1261 gcaccaatgc tacaagcatt gtttgaaaat cgtaaagcta ttatttactt cctatatggt
     1321 ctggtggcaa aagttatttt acaagttccc tccattttcc ttttccatgc ctatggacca
     1381 cttttctcaa cgacagtagc tctttgtatc ccagtaattc ttatgtattt gaaaatacat
     1441 gaaattacag gatttaaacg ccaagctatt cgtcgtacaa gtgctttagt ccttattcta
     1501 acgctattga tgagctttat tatttctatg ataatatggt taatgaacct ggttattgta
     1561 cccgatagta gactggtaag tctagtttac attatcgtta tcggtgcaat aggtttaggg
     1621 gtatatggct ttatggcact ggccacccat ctacttgata agatgattgg tagtcgtgct
     1681 caagacttac gccgaaagtt gcaccttaac taatgaaaaa ataggttaga cagaattgtt
     1741 taacctagtt ttttgacgac caaaattatt tgtgaaattt taaggtagtc acaatatagt
     1801 gagcttattt ttatgttata attaaatgga gaaatatgag gtgaaagatg tcttacgaaa
     1861 agcttttaga acgattttta acatacgtca aaataaatac aagaagtaat cctaatagta
     1921 cacaaacgcc aacaactcaa agtcaagttg actttgcttt aacagtttta aaaccagaaa
     1981 tggaagcaat tggtttaaaa gatgttcatt atttaccttc taatgggtat ttggttggaa
     2041 ccttacctgc tacaagcgac cgcttacgcc ataaaatagg ttttatatcc catatggata
     2101 cagctgattt caatgctgaa aatattactc cacaaattgt tgactataaa ggtggagata
     2161 ttgaacttgg agactcaggt tacattttaa gtccgaaaga ttttccaaat ttaaataatt
     2221 accatgggca aacattgatt acaacagatg gtaaaacctt actgggagca gacgataagt
     2281 ctggtatagc agaaatcatg acagctatgg aatatttggc ttcgcatcca gaaattgagc
     2341 actgtgaaat tagagttggc tttggaccag atgaagaaat tggtataggt gcagataaat
     2401 ttgatgttaa agattttgat gttgattttg cctatacagt ggatggtgga ccactaggag
     2461 aattacagta tgaaaccttt agtgcagctg gtttggagct tacatttgaa ggacgaaacg
     2521 ttcaccctgg aactgcaaaa aatcaaatga ttaatgcttt acagcttgct atagattttc
     2581 atagtcaatt accagaaaat gaacgtcctg aacaaacaga tggctatcaa ggattttatc
     2641 acttatatga tttaagtgga acagttgatc aagctaaaag ttcatatatc attcgagatt
     2701 ttgaggaagt taatttctta aaacgtaagc aattggctca agctatcgct gataatatga
     2761 atgaagcatt acaatctgaa cgtgtaaagg ttaaactata cgatcaatat tacaacatga
     2821 agaaagttat tgaaaaagac atgacaccta tcaacattgc taaagaagta atggaagagt
     2881 tagacatcaa gccgatcata gaaccgattc gtggtggtac agatggctct aaaatttcct
     2941 ttatgggaat ccctactcct aatctttttg caggtggtga aaacatgcat ggacgctttg
     3001 aattcgttag tctacaaaca atggaaaaag cagttgatgt tattttaggc atcgttgcta
     3061 aggattagta aagaaatatc agattggagt ggttaaatga taaaattatt tgggaagata
     3121 cgttatcact ggcaacctga actttcttgg gctattattt attggtcaat agccattgca
     3181 ccaattttta ttggtttatc tctgctatat gaaagaactg agattccgag tcaggtattt
     3241 gttctatttg ccacctttat tgttttagta ggcattggat ttcatcgcta ttttgttatt
     3301 gaagaagatg gatatcttag gattgtatct tttaactttt taagacgatc taagtttcca
     3361 attgaagata ttgctaaaat tgaggtcact aaatcaagtg tcactatcaa atttaataat
     3421 aaccatgagc gtatttttta catgcgaaaa tggcctaaaa aatatttttt agatgcacta
     3481 gctattgaac ctaaatttaa gggagaagtg gagcttctcg ataatttgat taaaatggat
     3541 tattttgaat gttatcgcta cgataaaaaa gctcttacaa aatcgtaaga gctttggtgg
     3601 gacatgattt aactgctaaa acagtatctt gatcagaact tggaatttct ttttctaggt
     3661 tatctgtatc ggaaaattta acgataccat catcttgata atcaaaaata tttgaatatg
     3721 tttggcaaag cccgcaggca atacattttt ctggaattag tgcaactttc atagtattat
     3781 tgtaatactt attgatacga ttaacaaggg ggaagtcatg gcgaaaaaac catgggaaaa
     3841 aaaagttgtg gaaaacaata gtcatagaaa agacaaaatt acaagaacga gtagaggtgt
     3901 ggtgtcaagt actccttgga ttaccgcttt cttgagtgca ttctttgtta ttgtcgttgc
     3961 tattttattt atagtatttt atacttcaaa tagaggagaa gacagagcaa aagaaacttc
     4021 tggtttttat ggtgcttcaa gtcaaaaagt taatagttct aaaacgaaaa aagcatctac
     4081 aagtaagaaa acaacgtcat catcaaaaac taaaactagt gataaatcaa caacatcttc
     4141 tagtacagta gcaagttcga gtgagacatc gacatcatct tcagtaaacg gtggaacaat
     4201 cgtagttcaa ccaggtgagg gagcagcctc catagctgct cgtgcaggta tctcagttgc
     4261 tcgtttacaa gaacttaatc ctggtcatat gacctcaggg tattggtatg ctaatccagg
     4321 cgatcctgtg gttgtaaatt aaggggagga ttatgaactc aattaatatt gctattgatg
     4381 gaccagcatc aagtgggaaa agtactgtcg ctaagattat tgctaaaaat ttgaattaca
     4441 cttaccttga tacaggtgct atgtatcgtt gtgctactta tctggcttta cagcatggat
     4501 atgaagcaca agatgtttcg aaaattttag gactcctagc tgaaagaccg atttcttttg
     4561 gtaaagcaga agatggcagc cagacagtct ttcttggcac agaagaagtt actttagcta
     4621 tccgtcaaaa cgatgttact aacaatgtct cgtgggtttc tgctatccca gagattcgag
     4681 aagagttagt aaaccagcag cgtcgtatag ccaaagatgg tgctattata atggatggtc
     4741 gtgacatagg aacagttgtt ttgccagatg cagaattgaa gattttttta gtggcttctg
     4801 ttgatgaaag agctgagcgt cgctttaaag aaaatcaaga aaaaggcatt gagagtgatt
     4861 ttgaaacact gaaatcagaa attgctgctc gtgattataa agatagccat cgagaagttt
     4921 caccattaaa ggcagcagaa gatgctattg aatttgatac tacaggagta tcaattgaag
     4981 gtgttgtaac ttttattcaa gaaaaagcag aaaaaataat tgacatgaag aattagtcat
     5041 gatatactag ttgcaatgag aaaagcagaa gtgagaactt ctcgccttgc gacttaggtt
     5101 gtctggccca acatgtcaag attccatcta ggaagattat atgtgcgggc ttgatttatc
     5161 aagtccgctt tcgtttttct ctaaaaaaat aaagaggtga agatcatagc taaaaaagat
     5221 ctattcatta acgatgaaat tcgcgttcgt gaagttcgtc tggttggtct cgagggtgaa
     5281 caattaggta ttaaaccatt gtctgaagct caagcaattg cagatgatgc taatgttgat
     5341 ttggttttaa ttcagccaca agctacacca cctgttgcga aaattatgga ctatggtaag
     5401 ttcaaatttg agtatcaaaa gaaacaaaaa gaacaacgta aaaaacaaag cgttgttact
     5461 gttaaggaag ttcgtcttag cccagttatt gataaagggg attttgaaac aaaacttcgt
     5521 aacggtcgta agttccttga aaaaggtaac aaagttaaag tttctatccg attcaaagga
     5581 cgtatgatta ctcataaaga aattggagca aaggtattgg cggaatttgc tgaagcgaca
     5641 caagatatcg ctattattga gcaaagagct aagatggatg gtcgccaaat gtttatgcag
     5701 cttgcaccaa ttccagacaa gaaataattg tcgactttat agtattattt aagaggagaa
     5761 tttaaaatgc caaaacaaaa aacacaccgc gcatcagcta aacgctttaa acgcacaggt
     5821 tcaggtggat tgaaacgctt ccacgcgttt acttcacacc gtttccacgg taaaactaaa
     5881 aaacaacgtc gtcatcttcg taaagctact atggtttcat caggagattt caaacgtatt
     5941 aaagcaatgc ttactcgcct taaataataa ggtctctgta aacatttact acatattata
     6001 aaggattatt tggaggaaat ataaatggct cgtgttaaag gtggagttgt ttcacgtaaa
     6061 cgtcgtaaac gcgtacttaa attagctaaa ggttactatg gtgcaaaaca tatcttgttc
     6121 cgtactgcaa aagaacaagt aatgaattct tactactatg cataccgtga ccgtcgtcag
     6181 aaaaaacgtg acttccgtaa attatggatc actcgtatca atgcggctgc tcgtatgaat
     6241 ggtttatcat attcacaatt gatgcacggt ttgaaattag ctgaaatcga agttaaccgt
     6301 aaaatgcttg ctgatttagc agttaacgat gcagcagctt tcacagctct tgcagatgca
     6361 gctaaagcta aacttggtaa ataagaaata aaaaagttgg gtctgcctaa cttttttta
//
LOCUS       DBJYSH010000103         6406 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.103, whole genome shotgun sequence.
ACCESSION   DBJYSH010000103 DBJYSH010000000
VERSION     DBJYSH010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 6406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6406
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.103"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            complement(<1..623)
                     /gene="serB"
                     /locus_tag="ACUBV3_001686"
     CDS             complement(<1..623)
                     /gene="serB"
                     /locus_tag="ACUBV3_001686"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HHU3848173.1"
                     /translation="MIKGLLVMDVDSTLIMEEAIDLLAIEAGVGKQVAALTDAAMRGE
                     LDFEEALKKRVALLKGLPVTILTDILSSIHFTPGAYELIKECHKRQMKVGLVSGGFHE
                     TIDILAKQLQVDYVKANRLGVKGGFLTGEVEGEIVTKEVKKIKLKEWASENHLDLSQT
                     IAMGDGANDLPMIKSAGVGIAFCAKPIVREEAAYQINEKNLLKVFDI"
     gene            complement(717..2441)
                     /gene="ezrA"
                     /locus_tag="ACUBV3_001687"
     CDS             complement(717..2441)
                     /gene="ezrA"
                     /locus_tag="ACUBV3_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HHU3848174.1"
                     /translation="MSSGIILLLVAIVLLVIIAYVVGVVIRKRNDTLIANLETRKQEL
                     VDLPVQEEIEQVKLLHLIGQSQSTFREWNQKWTDLSTNSFKDIDFHLVEAENLNDSFN
                     FVRAKHEIDNVDSQLTIIEEDIVSIREALEVLKEQEEKNSARVTHALDLYETLQKSIS
                     EKEDNYGTTMPEIEKQLKNIEAEFSHFVTLNSTGDPIEASEVLNKAEEHTIALGQITE
                     QIPAIVAKLEDDFPDQLDDLETGYRRLLEENYHFPEKDIEQRFQEVREAIRSNSDGLV
                     SLDLDRARDENEHIQEKIDKLYDIFEREIAAYKVAHKDSKIIPQFLAHAKSNNEQLGH
                     EIKRLSAKYILNENESLSLRSFTNDLEEIETKVLPSVENFGQEASPYTHLQILFERTL
                     KTLTTVEENQMEVFEAVKTIESVETRARQNMDKYVNKLHMIKRFMEKRNLPGIPQDFL
                     STFFTTSSQIEALINELSRGRIDIEAVSRLNDVTTNAIANLEQATYLVVQDATLTEQL
                     LQYSNRYRSFEQNVQKSFEQALYLFEVEHNYKASFDEISYALETVEPGVTDRFVTSYE
                     KTQERIRF"
     gene            complement(2535..4487)
                     /gene="gyrB"
                     /locus_tag="ACUBV3_001688"
     CDS             complement(2535..4487)
                     /gene="gyrB"
                     /locus_tag="ACUBV3_001688"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002283665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HHU3848175.1"
                     /translation="MTEETKNMEQRAQEYDASQIQVLEGLEAVRMRPGMYIGSTSKEG
                     LHHLVWEIVDNSIDEALAGFAGHIKVYIEPDNSITVVDDGRGIPVDIQEKTGRPAVET
                     VFTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKNGKIHYQEYQRGVVV
                     NDLEIIGDTDLSGTTVHFTPDPEIFTETTVFDFDKLAKRIQELAFLNRGLRISISDKR
                     EGQEVEKEYHYEGGIGSYVEFINENKEVIFENPIYTDGELDGISVEVAMQYTTGYQET
                     VMSFANNIHTHEGGTHEQGFRTALTRVINDYAKKNKILKENEDNLTGEDVREGLTAVI
                     SVKHPNPQFEGQTKTKLGNSEVVKITNRLFSEAFNRFLLENPQVAKKIVEKGILASKA
                     RIAAKRAREVTRKKSGLEISNLPGKLADCSSNNAEMNELFIVEGDSAGGSAKSGRNRE
                     FQAILPIRGKILNVEKATMDKILANEEIRSLFTAMGTGFGADFDVSKVRYQKLVIMTD
                     ADVDGAHIRTLLLTLIYRFMRPVLEAGYVYIAQPPIYGVKVGSEIKAYIQPGVNQEEE
                     LRQALDTYSSGRSKPTVQRYKGLGEMDDHQLWETTMDPENRLMARVSVDDAAEADKIF
                     DMLMGDRVEPRREFIEANAVYSNLDI"
     gene            complement(4488..5048)
                     /locus_tag="ACUBV3_001689"
     CDS             complement(4488..5048)
                     /locus_tag="ACUBV3_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001106189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HHU3848176.1"
                     /translation="MNYHDYIWDLGGTLLDNYESSTRAFVETLKEFGYQADHDSVYQK
                     LKESTADAIAYFIPEEADFLKEYKANEAKVLETPILFQGAKELLAKIQRQGSRNFLVS
                     HRDNQVIVILEKTEIIDYFTEVVTADNGFSRKPSPESMLYLKEKYQIDNCLVIGDRDI
                     DKQAGESAGFDTLLVDGSKSLMEIIE"
     gene            complement(5167..6372)
                     /locus_tag="ACUBV3_001690"
     CDS             complement(5167..6372)
                     /locus_tag="ACUBV3_001690"
                     /EC_number="2.4.99.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0015648 - lipid-linked peptidoglycan
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HHU3848177.1"
                     /translation="MSQKSNYFKGQIDYAVVIPVFFLLMIGLASIYVATMNDYPSNIY
                     IAMFQQVSWIIMGCIIAFVVMLFSTEFLWKATPYLYALGLTLMVLPLIFYSPQLFAAT
                     GAKNWVTIGSVTLFQPSEFMKISYILMLSRITVSFHQKNRKTFQDDWKLLGLFGLVTL
                     PVMILLMLQKDLGTALVFLAILSGLILLSGISWWIILPILSTIVLFIASFLMIFISPN
                     GKEWFYNLGMDTYQINRLSAWIDPFSFADSIAYQQTQGMVSIGSGGLTGKGFNILELS
                     VPVRESDMIFTVIAENFGFIGSAIVLGLYLIIIYRMLRITIESNNQFYTFISTGFIMM
                     IVFHVFENIGAAVGILPLTGIPLPFISQGGSSLLSNLIGIGLVLSMSYQNTVRQAKAN
                     GSHLKYRSI"
BASE COUNT         2023 a         1313 c          956 g         2114 t
ORIGIN      
        1 ataatatcaa ataccttaag caaatttttt tcattaatct gatacgcagc ttcttctctg
       61 acaattggtt ttgcacaaaa agcaattcct accccagcgg atttgatcat tggtaaatca
      121 ttggcaccat ctcccattgc gatggtttgt gaaaggtcta agtgattttc agaagcccac
      181 tcttttaact ttattttttt cacttcttta gtgacaatct ctccctcaac ttcgccagtg
      241 agaaaaccac ctttgacacc aagacgattc gctttgacat aatcaacttg taattgctta
      301 gctaagatat caatcgtctc gtgaaaacct cctgatacaa gaccaacttt catctgacgc
      361 ttgtgacact ctttaatcaa ttcataagcc cctggagtaa aatgtattga ggataatata
      421 tctgtcaaaa tagtaacagg caaaccttta agtagcgcta ctcttttctt caaagcttct
      481 tcaaaatcta actcgccacg cattgcagcg tcagtcagtg cagcaacctg tttccccacc
      541 cctgcttcaa tagctaataa atcaatagct tcttccatta ttaaggttga atcaacgtcc
      601 atgactaaca aacctttaat catttaaaac ctccaaaact tttttactat tgtaccttaa
      661 aattagcacc tttcatagat agcaattaaa aaaagaagag tactaactct tcttttttag
      721 aaacgaatac gttcttgtgt cttttcataa gacgtcacaa aacggtcagt tacacctggt
      781 tccacagtct caagtgcgta tgaaatttca tcgaaagatg ctttatagtt atgttcaact
      841 tcaaataagt acaaagcttg ttcaaagctc ttttgcacat tttgttcaaa actacgataa
      901 cggtttgaat actgtagtaa ctgctctgtg agagtcgcat cttgaacaac tagatatgta
      961 gcttgctcta aattagcgat agcatttgta gtcacatcat ttaaacgtga aacagcctca
     1021 atatcaatac gaccgcgact gagttcatta ataagtgcct caatttgaga acttgttgta
     1081 aagaaagtgc ttaagaagtc ttgcgggatt cctggaagat tgcgtttttc catgaaacgt
     1141 ttaatcatat ggagcttgtt aacatactta tccatatttt ggcgtgctcg tgtctcaacg
     1201 ctttcaatag ttttaacggc ttcaaaaact tccatttgat tttcttcaac agtagtcaat
     1261 gttttcaacg tacgctcaaa taaaatttgt agatgagtat aaggtgaagc ttcttgacca
     1321 aaattttcta cagaaggtaa aacctttgtt tcaatttctt ccaaatcatt tgtgaaggaa
     1381 cgtaaactta agctttcatt ttcatttaag atatatttag cagataatcg tttgatttca
     1441 tgccccagtt gttcattgtt agattttgca tgagcaagga attgaggaat aattttacta
     1501 tccttatgag ctactttata agcagcaatc tcacgttcaa aaatatcata aagtttatca
     1561 attttttctt gaatatgttc attttcatcg cgagcacgat ctagatccaa actaactaaa
     1621 ccatctgaat tcgaacgaat agcctcacga acttcttgaa aacgttgttc aatatccttt
     1681 tcagggaaat ggtagttttc ctctaaaaga cgacgatatc ctgtctccaa atcatctaac
     1741 tgatctggaa aatcgtcttc taatttagca acaatagctg gaatttgttc tgtaatttgc
     1801 cctaaagcaa tcgtatgctc ctctgcttta ttcaaaactt cagaagcttc aatcgggtca
     1861 ccagttgagt ttaaggtcac aaaatgtgaa aattcagcct cgatattttt caactgcttt
     1921 tcaatttctg gcatggttgt cccgtaatta tcttcttttt cagaaattga tttttgaagt
     1981 gtttcgtata ggtccaaagc atgtgttaca cgggcactat ttttctcttc ttgctctttt
     2041 aaaacttcta aagcttcacg aatcgaaaca atatcttctt ctataatagt tagctgacta
     2101 tcaacattat caatctcgtg cttagcacgc acaaaattaa atgaatcatt aagattttca
     2161 gcttcaacta aatgaaaatc aatatcttta aatgaattag ttgataaatc agtccatttt
     2221 tggttccatt ctctaaaagt tgattgactt tggccaatca aatgaagcaa tttgacttgc
     2281 tcaatctcct cttgcacagg aaggtcaact aactcttgtt ttctagtttc taagtttgca
     2341 attaatgtat cattacgttt tcttattacg acgccgacta cgtaggcaat aatcacaagc
     2401 aggactatag ccacaagcag caatatgatt ccgctcgaca ttcaactctc cttataatgg
     2461 tctaattttc taaaaatcta ctataacgtt aagattatag catacttgaa ctgtttttca
     2521 aagctatctt tggactagat atctaagtta ctataaacag cattagcttc aatgaattca
     2581 cgtcgtggtt ctactcgatc tcccattagc atatcgaaaa tcttatctgc ttctgctgca
     2641 tcatcaactg agacacgtgc catcaagcgg ttttcaggat ccattgttgt ttcccaaagt
     2701 tgatggtcat ccatctcacc tagacctttg taacgttgta cagtaggttt tgaacgtcct
     2761 gaactatagg tatctagagc ttgacgtaat tcctcttctt gattgacccc tggctgaata
     2821 taagctttaa tctcacttcc tactttgaca ccatagattg gtggttgagc gatataaaca
     2881 tatcctgctt ccaaaacagg tcgcataaaa cgataaatca gagttaaaag taaagttctg
     2941 atgtgagctc catcaacatc ggcatcagtc ataatgacta atttttgata tctgactttt
     3001 gatacatcaa aatctgcacc aaatccagtt cccatagctg tgaagagact tcggatttct
     3061 tcgtttgcta aaattttatc cattgtagct ttttcaacgt ttagaatctt acccctaatc
     3121 ggtaaaattg cttgaaactc acgattacgt cctgattttg cagaccctcc cgcagaatct
     3181 ccctccacta taaaaagttc attcatctca gcattattag atgagcaatc agccagtttc
     3241 ccaggtaagt ttgaaatctc taagcctgac tttttacgtg ttacttcacg cgcccgtttt
     3301 gctgcaatac gggctttaga tgccaaaatt cctttttcaa caatcttctt agctacttgt
     3361 ggattttcta ataggaatcg attgaatgct tcgctaaaga gacgatttgt aattttaaca
     3421 acttcagaat ttccaagttt tgttttagtt tgtccttcaa attgtggatt tggatgttta
     3481 acagatataa ctgctgttaa accttcacga acatcttctc cagttaaatt atcttcattt
     3541 tctttgagaa tcttattctt tttagcgtaa tcattaatca cacgtgtcaa ggcagtacga
     3601 aatccttgtt cgtgcgttcc tccttcatga gtgtggatgt tatttgcaaa actcataaca
     3661 gtctcttgat atccagtagt atactgcatt gctacttcaa ctgaaatacc atctaattca
     3721 ccgtcagtat aaataggatt ttcaaaaatc acttctttgt tctcattgat aaactcaacg
     3781 tatgaaccta taccaccttc atagtgatat tctttttcaa cttcttgacc ttctcgcttg
     3841 tctgaaatgg agattcttag tccacgattt aaaaaagcta attcctgaat acgtttggct
     3901 aatttatcaa aatcaaatac agtggtttca gtgaaaattt ctgggtcagg cgtaaagtga
     3961 actgttgttc ctgataagtc agtatcaccg ataatttcaa gatcattaac aacaacaccg
     4021 cgttggtatt cttgataatg aatcttgcca tttttataaa ccttgacatc taattgtgtt
     4081 gacaatgcat taacaacaga tgaccctaca ccatgtaagc caccagaaac cttgtatccg
     4141 ccaccaccaa atttaccacc ggcatggaga actgtaaaaa ctgtctctac agctggtctt
     4201 ccagtttttt cttggatatc taccggaata ccgcgaccat catcaacaac tgtaatagaa
     4261 ttatctggtt caatgtaaac tttaatatgt cctgcaaatc cagctaaggc ttcatcaatt
     4321 gagttatcaa caatttccca tacaaggtga tgaagccctt ctttcgaagt cgaaccgata
     4381 tacatgcctg gacgcatgcg aacagcttct aaaccttcta atacttgaat ctgacttgcg
     4441 tcatattctt gagcacgttg ttccatattc tttgtttctt ctgtcattta ctctattatc
     4501 tccatcaatg attttgaccc atcgactaat aaggtatcaa aaccagccga ttctcctgct
     4561 tgcttatcta tatcacgatc gcctattact aaacaattat caatttggta tttttctttc
     4621 aaataaagca tactctcggg actaggtttc cttgaaaagc cgttatcagc agtcactact
     4681 tctgtaaaat agtcaataat ttctgttttt tccagaataa ctataacttg attatcacga
     4741 tgagacacca aaaaattacg actaccttgt ctttgaattt tcgctaaaag ttcttttgcc
     4801 ccttggaata gaattggtgt ttccaaaacc ttagcttcat tagccttata ctcttttaaa
     4861 aaatcagctt cttcagggat aaaatatgca attgcatctg ctgttgactc ttttaatttt
     4921 tgatacacac tatcgtggtc tgcttgatat ccaaattctt tcaaagtctc tacaaaagca
     4981 cgtgtggaac tttcgtaatt atcaagtagt gtaccaccca aatcccaaat ataatcatga
     5041 taattcatac cctttattat atcatattat tacgattttt tatagctacc accttactta
     5101 attaactcac tcctctaaaa tatttcgatt attaaaaggc tggattatct ttccaacctt
     5161 tttttgttat atactacgat acttcaaatg tgatccatta gccttggcct gacgaactgt
     5221 attttgataa gacatggata agaccaatcc aattccaata agattactta gtaatgaact
     5281 tccaccttgc gaaataaacg gaagtgggat tcctgttaga ggtaatatac caactgctgc
     5341 accaatattt tcaaaaacat ggaaaacaat catcatgata aaaccagttg atataaaagt
     5401 ataaaattga ttattagatt caatggtgat ccttaacata cggtaaatga taatgagata
     5461 taaccctaaa acaatcgccg aaccaatgaa gccaaaattc tcagcaatga cagtaaaaat
     5521 catgtcactt tcacgaacag gaaccgagag ctctaaaata ttaaaacctt tacctgttaa
     5581 cccgccactt ccaattgata ccataccctg tgtttgttga taagcaattg agtctgcaaa
     5641 actaaatgga tcaatccaag cagataaacg gttgatttga tatgtatcca tccccaaatt
     5701 ataaaaccat tccttgccgt ttggtgagat aaaaatcatc aaaaagctag ctataaaaag
     5761 aacgatagta gataaaattg gcaagattat ccaccaagat atgcccgata aaaggattaa
     5821 acctgacaaa atggcaagga aaactaatgc tgttcctaaa tctttttgta acattagaag
     5881 aatcatcact ggcaatgtaa ccaaaccaaa caatcctagc aacttccaat catcttgaaa
     5941 agttttgcga tttttctgat gaaaactaac agttattcga gataacatta aaatatagga
     6001 aatcttcata aactctgacg gttgaaacaa agttaccgag ccaatggtca cccaattttt
     6061 agcacctgtt gccgcaaaaa gctgtggact ataaaaaatt aagggaagaa ccattagggt
     6121 tagtcctaaa gcatacagat agggcgtggc tttccataaa aattcagtac tgaaaagcat
     6181 aacgacaaat gcaataatac accccataat aatccaagac acctgctgaa acatcgcgat
     6241 atagatatta gaaggataat cattcatcgt tgcaacataa attgaggcta aaccaatcat
     6301 taataaaaaa aatactggta tcacaacagc ataatcaatt tgccctttaa aatagtttga
     6361 tttttgtgac ataggacttc cttttttatt catggtaatt attata
//
LOCUS       DBJYSH010000104         6372 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.104, whole genome shotgun sequence.
ACCESSION   DBJYSH010000104 DBJYSH010000000
VERSION     DBJYSH010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 6372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6372
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.104"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            2..1372
                     /gene="nox"
                     /locus_tag="ACUBV3_001691"
     CDS             2..1372
                     /gene="nox"
                     /locus_tag="ACUBV3_001691"
                     /EC_number="1.6.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H2O-forming NADH oxidase"
                     /protein_id="HHU3848178.1"
                     /translation="MSKIVVVGTNHAGTAAIKTMLSNYGEANEIVTFDQNSNISFLGC
                     GMALWIGEQIDGPEGLFYSDKEQLESMGAKVYMNSPVLNIDYDKKEVTALVDGKEHVE
                     SYEKLILATGSQPIIPPIKGVEIQEGSREFKATLENLQFVKLYQNSEEVIEKLAKPGI
                     NRVAVVGAGYIGVELAEAFQRIGKEVTLVDVADTCMGGYYDRDFTDMMSKNLEDHGIR
                     LAFGQAVQAVEGDGKVERLVTDKETFDVDMVILAVGFRPNTELGAGKLDTFRNGAWVV
                     DKKQETSVKDVYAIGDCATIWDNSRDDINYIALASNAVRTGIVAAHNACGTELEGAGV
                     QGSNGISIYGLNMVSTGLTLEKAKQAGYNAVETGFNDLQKPEFIKHNNHEVAIKIVYD
                     KDSRVILGCQMVSHEDVSMGIHMFSLAIQEKVTIEKLALTDIFFLPHFNKPYNYITMA
                     ALGAKD"
     gene            complement(1493..2482)
                     /locus_tag="ACUBV3_001692"
     CDS             complement(1493..2482)
                     /locus_tag="ACUBV3_001692"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019768616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HHU3848179.1"
                     /translation="MTATKQHKKVILVGDGAVGSSYAFALVNQGIAQELGIIEIPALF
                     DKAVGDAEDLSHALAFTSPKKIYAATYADCADADLVVITAGAPQKPGETRLDLVGKNL
                     AINKSIVTQIVESGFNGIFLVAANPVDVLTYSTWKFSGFPKERVIGSGTSLDSARFRQ
                     ALADKIGVDARSVHAYIMGEHGDSEFAVWSHANVAGVQLEQWLQENRDIDEQGLVDLF
                     ISVRDAAYSIINKKGATYYGIAVALARITKAILDDENAVLPLSVYQEGQYGDVKDVFI
                     GQPAIVGAHGIVRPVNIPLNDAELQKMQASAEQLKDIIDEAWKNPEFQEASKN"
     gene            2721..5180
                     /gene="gyrA"
                     /locus_tag="ACUBV3_001693"
     CDS             2721..5180
                     /gene="gyrA"
                     /locus_tag="ACUBV3_001693"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HHU3848180.1"
                     /translation="MQDKNLVDVNLTSEMKTSFIDYAMSVIVARALPDVRDGLKPVHR
                     RILYGMNELGVTPDKPHKKSARITGDVMGKYHPHGDSSIYEAMVRMAQWWSYRHMLVD
                     GHGNFGSMDGDGAAAQRYTEARMSKIALEMLRDINKNTVDFQDNYDGSEREPLVLPAR
                     FPNLLVNGATGIAVGMATNIPPHNLGESIDAVKLVMDNPDVTTRELMEVIPGPDFPTG
                     ALVMGRSGIHRAYETGKGSIVLRSRTEIETTSNGKERIVVTEFPYGVNKTKVHEHIVR
                     LAQEKRIEGITAVRDESSREGVRFVIEVRRDASANVILNNLFKLTSLQTNFSFNMLAI
                     EKGVPKILSLRQIIDNYIEHQKEVIVRRTQFDKAKAEARAHILEGLLVALDHLDEVIT
                     IIRNSETDTIAQAELMSRFELSERQSQAILDMRLRRLTGLERDKIQSEYNDLLALIAD
                     LADILAKPERVVTIIKEEMDEVKRKYADARRTELMIGEVLSLEDEDLIEEEDVLITLS
                     NKGYIKRLAQDEFRAQKRGGRGIQGTGVNNDDFVRELVSTSTHDTVLFFTNLGRVYRL
                     KAYEIPEYGRTAKGLPIVNLLKLDEGETIQTIINARKEDVANKYFFFTTQQGIVKRTS
                     VSEFSNIRQNGLRAINLKENDELINVLLIDENEDVIIGTRTGYSVRFKVNAVRNMGRT
                     ATGVRGVNLREGDKVVGASRIVNGQEVLIITEKGYGKRTEASEYPTKGRGGKGIKTAN
                     ITAKNGPLARLVTINGNEDIMVITDTGVIIRTNVANISQTGRSTMGVKVMRLDQEAKI
                     VTVALVEQEIEDKSNIEDTKE"
     gene            5187..5930
                     /locus_tag="ACUBV3_001694"
     CDS             5187..5930
                     /locus_tag="ACUBV3_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HHU3848181.1"
                     /translation="MRNKKKSHGFFNFVRWLLVVLLIIVGLALVFNKPIRNAFIAHQS
                     NHYQISRVSKKTIEKNKKSKTSYDFSSVKSISTESILSAQTKSHNLPVIGGIAIPDVE
                     INLPIFKGLGNTELSYGAGTMKENQIMGGPNNYALASHHVFGLTGSSKMLFSPLEHAK
                     KGMKVYLTDKSKVYTYTITEISKVTPEHVEVIDDTPGKSQLTLVTCTDPEATERIIVH
                     AELEKTGEFSTADESILKAFSKKYNQINL"
     gene            5946..6359
                     /gene="gloA2"
                     /locus_tag="ACUBV3_001695"
     CDS             5946..6359
                     /gene="gloA2"
                     /locus_tag="ACUBV3_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMU1112c/YaeR family gloxylase I-like
                     metalloprotein"
                     /protein_id="HHU3848182.1"
                     /translation="MKLKAVHHIAIIVSDYEKSKDFYVNKLGFEIIRENHRPERHDYK
                     LDLRCGDIELEIFGNRLDDPEYETPPQRIGRPNWPREACGLRHLAFYVPDVEAYKVEL
                     ENLGIFVEPIRYDDYTGKKMTFFFDPDGLPLELHE"
BASE COUNT         2064 a         1042 c         1320 g         1946 t
ORIGIN      
        1 tatgagtaaa atcgttgtcg tagggacaaa ccacgctggt acagcagcaa ttaaaacaat
       61 gcttagtaat tatggtgaag ccaatgaaat tgtaacattt gatcaaaatt ccaatatctc
      121 tttcttagga tgtggtatgg ctctttggat tggtgaacag attgatggtc cagaaggatt
      181 gttctactct gataaagagc agttggaaag tatgggagct aaggtttaca tgaactctcc
      241 agtacttaat attgactacg ataaaaaaga agttacagct ttagtagatg gaaaagaaca
      301 tgttgaaagt tatgaaaaac ttattttagc aactggttca caacctatca ttccaccaat
      361 taaaggtgtt gaaattcaag aaggatctcg tgaatttaaa gctactcttg aaaaccttca
      421 atttgtgaaa ctttatcaaa attcagaaga agttattgaa aagttagcta aacctggtat
      481 caatcgtgtt gctgttgttg gggctgggta tattggtgta gaattagcag aagctttcca
      541 acgtattgga aaagaagtaa cacttgttga tgttgctgat acttgtatgg gtggttacta
      601 tgatcgtgac tttactgata tgatgagtaa aaaccttgaa gaccatggta ttcgtcttgc
      661 tttcggacaa gctgttcaag cagttgaagg tgatggtaaa gtagaacgtc ttgttactga
      721 taaagaaaca tttgatgttg atatggtgat cttagctgtt ggattccgtc caaatacaga
      781 actcggtgca ggtaaattag atacattccg taatggcgct tgggtagttg ataagaagca
      841 agaaacaagc gtgaaagatg tttatgctat aggtgattgt gcgacaattt gggataattc
      901 tcgtgatgat atcaattata ttgctcttgc ttcaaacgcc gttcgtactg gtattgtagc
      961 agctcacaat gcatgtggta cagagcttga aggtgctggt gtccaagggt caaatggtat
     1021 ctcaatctat ggccttaaca tggtgtcaac tggtttgact ttagaaaaag caaaacaagc
     1081 tgggtacaat gcagttgaaa caggctttaa tgaccttcaa aaaccagaat ttatcaaaca
     1141 caataatcat gaagtagcta ttaagattgt ctatgataaa gatagccgag ttatccttgg
     1201 ttgccaaatg gtctcacacg aagatgtatc tatgggaatt catatgttct cacttgctat
     1261 tcaagaaaaa gttactattg aaaagcttgc tttgaccgat atcttcttct taccacattt
     1321 taataagcca tataactata ttacaatggc tgcattaggc gctaaagatt aatttaaaat
     1381 taaaaaggtt aagcgaaatg cttagccttt tatttcatcc tttaacaagg tcaaaaatgt
     1441 aacggcaaag aaaaagctaa gatattccta tcctagcttt ttttcaacta tgttagtttt
     1501 ttgatgcttc ttggaattct gggtttttcc aagcttcgtc aataatatct tttaattgtt
     1561 cagctgaggc ttgcattttt tgaagttcag catcatttaa tgggatatta actggacgaa
     1621 cgataccatg tgcacctacg attgcaggct gaccgataaa gacatcttta acatcaccgt
     1681 attggccttc ttgatataca gataatggaa gaactgcatt ttcatcatca aggatagctt
     1741 tagtaatacg agcaagtgcg acagcaatac cgtaatatgt agcacctttt ttgttgatga
     1801 ttgagtatgc agcgtcacga actgaaataa acaaatcaac aagtccttgt tcatcaatat
     1861 cgcgattttc ttgtaaccat tgttcaagtt ggacaccagc aacgttagca tgtgaccaaa
     1921 cggcaaattc tgagtcaccg tgttcaccca tgatgtatgc gtgaactgaa cgagcatcaa
     1981 caccaatttt atctgctaaa gcttgacgga aacgtgcaga gtcaagtgaa gtacctgaac
     2041 caataacgcg ttccttaggg aaacctgaga atttccaagt tgaatatgta agtacatcta
     2101 cagggttagc cgctactaag aagataccgt tgaaacctga ttcaacaatt tgagttacga
     2161 ttgatttgtt aattgctaag tttttaccaa caaggtcaag tcgagtttca cctggttttt
     2221 gaggtgcgcc agcagtaatg acaacaaggt cagcatctgc gcagtctgca taagtagctg
     2281 cgtagatttt tttaggtgat gtaaatgcaa gggcatgtga aagatcttca gcatcaccaa
     2341 cagctttatc aaataaagct gggatttcaa tgatacctaa ctcttgcgca ataccttggt
     2401 taacaagtgc aaaagcgtaa gaagaaccta cggcaccatc accaacgaga ataacttttt
     2461 tatgttgttt agttgcagtc atttctaaac atctccttaa atatttttgg gaagtttccc
     2521 atacacctat attctatcat tttcaatgaa agaagtcact aataaaatgt gaaaaaattt
     2581 gcaaacaaat tgaaatatca tagaaaacca ttgaaaacgc tgagtgtctt gatatttttg
     2641 ataccgattt atcatcgcaa ttttacctaa attatggtac aatgtaagag acttacagtg
     2701 aagaaaaggg gcatttttta atgcaagata aaaatttagt agatgttaat ctaactagtg
     2761 aaatgaaaac gagttttatc gattacgcca tgagtgtcat tgttgctcgt gcacttccag
     2821 atgttagaga tggtttaaaa cctgttcatc gtcgtatttt atatggtatg aatgaactag
     2881 gtgtgacacc tgataaacct cataagaaat cagcacgtat tactggtgat gttatgggta
     2941 aataccatcc acatggtgat tcatctattt acgaagcaat ggtgcgtatg gcacaatggt
     3001 ggtcatatcg tcatatgctc gttgatggtc atggaaactt tggttcaatg gatggggatg
     3061 gtgctgccgc acagcgttat acagaagcac gtatgagtaa aattgctctt gagatgcttc
     3121 gtgatatcaa taaaaataca gttgattttc aagataacta tgatggcagt gaacgtgaac
     3181 ctcttgtttt accagcgcgt ttccctaatt tattagtcaa tggtgcaact ggtattgctg
     3241 taggtatggc aacaaatatt ccaccacata atctaggtga gtctatagat gctgtcaaat
     3301 tggttatgga taaccctgat gttactacac gggagttaat ggaagttatt cctggtccag
     3361 attttcctac tggtgccttg gtgatgggac gttcaggtat tcaccgtgcc tatgaaactg
     3421 ggaagggatc aattgtctta cgttcacgga ctgaaattga aacaacttca aatgggaaag
     3481 agcgtattgt tgtcacagag tttccatatg gcgttaataa aacaaaagta cacgagcata
     3541 ttgtacgttt agcacaagaa aagcgcattg aaggtattac tgctgttcgt gatgaatcaa
     3601 gtcgtgaagg cgttcgtttt gttattgaag ttcgtcgtga tgcttcagca aatgtgattt
     3661 taaataatct tttcaaatta actagcttgc agacaaattt tagcttcaat atgcttgcta
     3721 ttgaaaaagg tgttccaaaa attttgtcat tacgacaaat cattgataac tatattgaac
     3781 accaaaagga agttattgtt cgtcgtactc agtttgacaa agctaaagca gaagcccgtg
     3841 ctcatatctt agaaggttta cttgtggcgc ttgatcactt ggatgaagtt atcacaatta
     3901 ttcgtaatag tgagacagac accattgctc aagcagagtt gatgtcacgt tttgaattat
     3961 cggagcgtca gagtcaagct attttagata tgcgtcttcg tcgtttaact ggattagaac
     4021 gcgataaaat ccaatcagaa tacaatgact tactagcttt aattgctgat ttagcagata
     4081 tcttagctaa gcctgaacgt gttgttacca ttataaaaga agagatggat gaggttaagc
     4141 gtaagtatgc tgatgcccgt cgtacagaat taatgattgg tgaagtttta tcacttgaag
     4201 atgaagacct tattgaggaa gaagatgttc ttattactct ctcaaacaag ggatatatca
     4261 agcgtctcgc tcaagatgag tttagagcac aaaaacgtgg aggacgcggt attcaaggga
     4321 ctggagttaa taacgatgat tttgttcgtg aattggtttc aacaagtact cacgatactg
     4381 tgcttttctt tacaaattta gggcgtgtgt atcgtttgaa agcatatgaa attccagaat
     4441 atggacgtac agctaaaggc ctccctatcg ttaacctttt aaaacttgac gaaggtgaga
     4501 caattcaaac aattattaac gctagaaaag aagatgtcgc taataaatat ttctttttca
     4561 caactcagca aggtatagtg aaacgtacaa gtgtttctga atttagtaac attcgtcaaa
     4621 atggacttcg tgctattaat ttgaaagaga atgacgagtt gattaacgtc ctcttgattg
     4681 acgaaaatga agatgttatt atcgggacac gtacaggcta ctctgttcgc ttcaaagtga
     4741 acgctgtacg taatatggga cgcacagcga ctggggtacg aggcgttaat cttcgtgaag
     4801 gtgataaagt tgtgggagct tctcgtatcg ttaatggaca agaagtacta atcatcactg
     4861 aaaaaggtta tggcaagaga acagaagctt cggaatatcc tacaaaaggc cgtggtggta
     4921 aaggaataaa aaccgctaat attactgcta aaaatgggcc attagcacgc ttagtgacga
     4981 ttaatggaaa tgaggacatc atggtcatta cagatacagg tgttattatt cgaaccaatg
     5041 ttgctaatat ttctcaaact ggtcgttcta ccatgggtgt taaagtgatg agacttgatc
     5101 aagaagctaa aatcgtcact gttgcgctag tggaacaaga aattgaagat aagtctaata
     5161 tagaggatac aaaagaatag gaagttatgc gtaataaaaa aaaatcacat ggtttcttta
     5221 actttgttag atggttattg gttgtattgc taattattgt tgggttggct ttggtgttca
     5281 ataaaccaat tcgcaatgct ttcatagctc atcaatcaaa tcattatcaa atttcacgag
     5341 ttagtaaaaa aacaattgaa aaaaataaga aatctaaaac aagttatgac ttttcatcag
     5401 tcaaatctat tagtacagaa tcgattttat ctgctcaaac gaaatcacat aatttgcctg
     5461 taataggagg gattgctata ccagatgtcg aaatcaatct cccaattttc aaaggactgg
     5521 gcaatacaga gttgagttat ggtgcaggaa cgatgaaaga aaaccagatt atgggaggcc
     5581 caaataacta tgccttagct agtcaccatg tctttggttt gacaggttct tctaagatgc
     5641 tcttttcacc tttagaacat gctaagaaag gtatgaaagt ttacctcact gataaaagca
     5701 aagtatacac ttacactatt actgaaattt caaaggtaac accagaacat gttgaggtta
     5761 ttgatgatac acctggcaag agccaactca ctttagtgac ctgtacggat ccagaagcca
     5821 cagaacgtat tattgtgcat gctgaattag aaaaaacagg tgaattttca acagcagatg
     5881 agtcgatatt aaaagcattt tcgaaaaaat ataatcaaat taatctctag aaggtagtta
     5941 gaattatgaa attaaaggct gttcatcata ttgcgataat tgtttctgac tatgaaaagt
     6001 caaaagactt ctatgttaat aagctaggat ttgaaattat tcgtgaaaac catcgtccgg
     6061 agcgtcatga ttataaatta gatttacggt gcggtgatat tgagcttgaa atatttggaa
     6121 atcgtcttga tgatccagaa tatgaaactc ctcctcagag aataggtagg ccgaattggc
     6181 cacgtgaagc atgtggttta cgacaccttg ccttttacgt tccagatgtg gaagcgtaca
     6241 aggtagaact ggagaattta ggaatttttg tagagcctat tcgttacgat gactatactg
     6301 gcaaaaagat gacctttttc tttgatccag atggtttacc tttagaatta catgaataat
     6361 aaacaaagtt gc
//
LOCUS       DBJYSH010000105         6291 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.105, whole genome shotgun sequence.
ACCESSION   DBJYSH010000105 DBJYSH010000000
VERSION     DBJYSH010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 6291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6291
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.105"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            59..1321
                     /gene="purD"
                     /locus_tag="ACUBV3_001696"
     CDS             59..1321
                     /gene="purD"
                     /locus_tag="ACUBV3_001696"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HHU3848183.1"
                     /translation="MKLLVVGSGGREHAIAKKLLASKDVDQVFVAPGNDGMTLDGLDL
                     VNIGISEHSRLIDFAKENEIAWTFIGPDDALAAGIVDDFNSAGLRAFGPTKAAAELEW
                     SKDFAKEIMVKYNVPTAAYGTFSDFEKAKAYIEEQGAPIVVKADGLALGKGVVVAETV
                     EQAVEAAQEMLLDNKFGDSGARVVIEEFLDGEEFSLFAFANGDKFYIMPTAQDHKRAF
                     DGDKGPNTGGMGAYAPVPHLPQSVVDTAVETIIKPVLEGMIAEGRPYLGVLYAGLILT
                     ADGPKVIEFNSRFGDPETQIILPRLTSDFAQNIDDIMMGIEPYITWQKDGVTLGVVVA
                     SEGYPLDYEKGVPLPEKTDGDIITYYAGAKFAENSKALLSNGGRVYMLVTTEGSVKAG
                     QDKIYTQLAQQDTTGLFYRNDIGSKAIK"
     gene            1602..2093
                     /gene="purE"
                     /locus_tag="ACUBV3_001697"
     CDS             1602..2093
                     /gene="purE"
                     /locus_tag="ACUBV3_001697"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HHU3848184.1"
                     /translation="MKTPIISIIMGSKSDWGTMQKTAEVLDNFGIAYEKKVVSAHRAP
                     DLMFKHAEEARGRGIKIIIAGAGGAAHLPGMVAAKTTLPVIGVPVKTRALSGLDSLYS
                     IVQMPGGVPVATMAIGEAGATNAALTALRILSIEDQNLADALAHFHEEQGKIAEESSN
                     ELI"
     gene            2080..3153
                     /gene="purK"
                     /locus_tag="ACUBV3_001698"
     CDS             2080..3153
                     /gene="purK"
                     /locus_tag="ACUBV3_001698"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HHU3848185.1"
                     /translation="MNLFKTIGIIGGGQLGQMMAIAAIYMGHKVITLDPASDCSASRV
                     SEVIVAPYDDVEALGQLAARCDVLTYEFENVDADGLDAVVSAGQLPQGTDLLRISQNR
                     IFEKDFLANKAGVTVAPYKVVTSSLDLEGFDLTKTYVLKTATGGYDGHGQKVIRSAED
                     LPEAQQLANSAECVLEEFVDFDLEISVIVSGNGQDVTVFPVQENIHRNNILSKTIVPA
                     RISDQLADKAKKMAVQIAKELQLSGTLCVEMFVTADDIIVNEIAPRPHNSGHYSIEAC
                     DFSQFDTHILGVLGQPLPPIKLHAPAVMLNVLGQHVQQAIDYVAENPSAHLHIYGKLE
                     AKHNRKMGHVTVFTEDADEVEEF"
     gene            3163..3420
                     /locus_tag="ACUBV3_001699"
     CDS             3163..3420
                     /locus_tag="ACUBV3_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazole carboxylase"
                     /protein_id="HHU3848186.1"
                     /translation="MIKIIVHAFINSGKIGVVEVLFATSDDKLLMTKMSEFQRHYPGD
                     YLAIYDLPLDTDLNKLSHYPSVAISKEDFHEEKANETNENS"
     gene            3786..4889
                     /locus_tag="ACUBV3_001700"
     CDS             3786..4889
                     /locus_tag="ACUBV3_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001867169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6287 domain-containing protein"
                     /protein_id="HHU3848187.1"
                     /translation="MASGEKAKIQRKKSGAWSMTYKTLDGNVVATFRPMFKKTGSHCY
                     ISTSKMTKSDGNNNFSIQIYYKGTSNVKIKMFDDNPEHTMQFSKKKFANNMLNRDEST
                     TNHSYDKVLEGDLTPFMGQFSSDSFNQTIVDSGMTYGGYEPKDYYEGRTTVFPAITKT
                     GYWNGITSHGSYVIKNDDLPKKIKNYYEIHVYGANSGAINSTGSLKRIFYLVPPKVEG
                     PDGKVLSKRQVFEVGANNQLISLSYQSPDWWKRYKKSGLEKDLDVDAIYRGDFSSLKG
                     TWRNGKGEVLTINDDGSTDDGERISIGSTEKSRLPSVGMSSGNFGVSVYLFQIGVSNP
                     YIENGHTSDTTRPRLAIGLNPGDFPTDAYYYRY"
     gene            4975..6273
                     /gene="purB"
                     /locus_tag="ACUBV3_001701"
     CDS             4975..6273
                     /gene="purB"
                     /locus_tag="ACUBV3_001701"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HHU3848188.1"
                     /translation="MLERYSRPEMAAIWTEENKYRAWLEVEILADEAWAELGEIPKED
                     VTKIREKADFDIDRILEIEQDTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLYKQANDIIRRDLENFTNIVAEKAKEHKFTIMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAAGVEAGKISGAVGNFANIPPFVEEYVCDKLGIHPQEISTQVLPRDL
                     HAEYFAVLASIATSIERMATEIRGLQKSEQREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MTGLARVIRGHMVTAYENVALWHERDISHSSAERIITPDTTILIDYMLNRFGNIVKNL
                     TVFPENMMRNMESTFGLIYSQRVMLKLIEKGMTREEAYDLVQPKTAYSWDNQVDFKPL
                     LEEDTKVTSCLTQEELDELFNPIYYTKRVDDIFERLGLEK"
BASE COUNT         1835 a         1218 c         1487 g         1751 t
ORIGIN      
        1 agtaatacgt ttttgttgca aatttctttt tatattattt aataggagtt attctgatat
       61 gaaattgctt gttgttggtt ctggtggtcg tgagcatgcg attgctaaga agttgttagc
      121 gtctaaggat gtggatcagg tttttgtggc acctggtaat gatggtatga ccttggatgg
      181 tctagacttg gtaaatatcg gaatttccga acattctagg ctaattgact ttgctaagga
      241 gaatgagatc gcttggacct ttattggtcc tgatgatgcg ctagcggctg gtatcgttga
      301 tgatttcaac agtgctggac tcagagcttt tggtccaacc aaggcagcag cggagctaga
      361 gtggtcaaaa gactttgcca aggaaatcat ggtcaaatac aatgttccaa cagcagccta
      421 tggcacattt tcagattttg aaaaagctaa agcctacatc gaagagcagg gcgcaccaat
      481 cgtggtcaag gctgacggct tggcgttagg taagggcgtg gtcgtggctg aaaccgttga
      541 acaggcggta gaggcggcgc aagagatgct tttagacaac aagtttggcg actcaggagc
      601 gcgcgtggtt atcgaggagt tcttggatgg tgaagagttc tccctctttg ccttcgctaa
      661 tggcgataag ttctacatca tgccgacagc tcaggatcac aagcgtgctt ttgacggaga
      721 caagggacca aacactggcg gtatgggtgc ctatgcgcct gttcctcatc tgcctcagag
      781 cgtggtggat acagcggttg agaccatcat taagcctgtc cttgagggca tgattgctga
      841 agggcgtcct tatctaggtg tcctctatgc ggggcttatc ctgacagctg atggccctaa
      901 ggttatcgag ttcaactcac gtttcggtga ccctgaaact cagattatcc tccctcgcct
      961 aacttccgat tttgctcaga acatcgacga catcatgatg ggcattgagc cttacatcac
     1021 ttggcagaag gacggcgtga ctttgggcgt tgtcgttgcc tcagaaggct atccgctcga
     1081 ttacgagaaa ggtgtgccac tgcctgaaaa gaccgacggc gacatcatca cctactatgc
     1141 aggagctaag tttgcggaaa atagcaaagc actgctctca aacggtggac gtgtctatat
     1201 gctcgtcacc acagaaggta gcgtcaaagc agggcaggac aaaatctata cccaactagc
     1261 ccaacaagac acaacaggcc tcttctaccg aaatgatatc ggaagcaaag ctattaagta
     1321 ataagactac tcattcaaat attagcgtcg gcaatcagcg accgaaggtc gctccaaaaa
     1381 gaaagaagaa aggaactgaa cacgggacta atttcctagg aaaaaaataa aactacctga
     1441 acgcaagcgt catcgttaga tttctgcgtc gtaggcgtct ggtgctaaag cacctagtcg
     1501 ccctgacgtg ggtgcatctt cgaaatcaaa gatttcggac ttaccgcata tttttctacg
     1561 gaaattttcg acaagtcgaa acgtcccttt gtatcttatt tatgaaaact ccaattattt
     1621 ctattatcat gggctccaaa tccgactggg gaaccatgca aaaaacggct gaagttctag
     1681 ataactttgg cattgcttac gagaagaaag tcgtctctgc ccaccgtgcg ccagacctca
     1741 tgttcaagca tgctgaggaa gcacgtggcc gtggtatcaa aatcatcatc gctggagcag
     1801 gtggggcagc tcacttgcct ggtatggtgg cagctaagac aacactacct gttattggtg
     1861 tgccggtcaa aacacgtgcc ctttcaggtt tggattcgct ctactctatt gtgcagatgc
     1921 ctggtggtgt gcctgtggcg accatggcta tcggagaagc gggagcgact aacgctgcct
     1981 tgacagccct ccgtatcctc tctattgagg accaaaatct ggctgatgcg ctagctcatt
     2041 tccatgagga acaaggaaaa atcgcggagg agtcaagcaa tgaacttatt taagaccatt
     2101 gggattatcg gtggtggtca gctggggcag atgatggcaa ttgcggctat ctacatgggc
     2161 cacaaggtca tcacgctgga tcctgctagt gactgctctg cctcccgcgt tagcgaagtg
     2221 attgtagcgc cttatgatga tgttgaggct ttgggacaat tagctgcgcg ttgcgatgtt
     2281 ttgacctatg agtttgagaa tgttgatgcc gacggtctgg atgccgttgt atcggctggt
     2341 cagctaccgc aggggactga tttgctccgc atttctcaaa accgtatctt tgaaaaagac
     2401 tttctggcaa ataaggctgg tgtgactgtc gctccttaca aggtggtgac gtctagcctt
     2461 gacctagagg ggtttgactt gaccaagacc tatgtcctca agacagcgac aggtggttat
     2521 gatggtcatg ggcaaaaggt tatccgctca gcagaagacc tgccagaggc gcagcaatta
     2581 gccaactcag ccgagtgtgt cttggaagag tttgtcgact ttgatcttga aatctcagtc
     2641 atcgtgtctg gaaatggtca ggatgtgacg gtctttcccg ttcaggaaaa tatccaccgc
     2701 aacaatatcc tatcaaaaac tatcgtacca gctcgcatct cagaccaact agctgacaag
     2761 gctaagaaaa tggcggtaca gattgccaag gaactccagc tatcaggaac cctctgtgtg
     2821 gaaatgtttg tgaccgcaga tgatatcatc gtcaatgaaa tcgccccacg cccccacaac
     2881 tcagggcact attctatcga agcctgtgac ttttcacagt ttgacactca tatcttgggt
     2941 gtgctggggc aacctctccc accaatcaag ctccatgccc cagccgtcat gcttaatgtc
     3001 cttggacaac atgtccagca ggcgattgac tatgttgccg aaaaccctag cgctcacctc
     3061 cacatatatg gtaaactaga agcgaaacat aaccgcaaga tgggacatgt gacggtgttt
     3121 acggaagatg cggatgaggt ggaggagttt taggaggttt gtatgattaa aatcattgtt
     3181 catgctttta ttaatagtgg taaaataggt gttgttgaag tattatttgc gacatcagat
     3241 gataaactct tgatgactaa aatgtcagaa tttcagcgcc actacccagg tgattattta
     3301 gctatttatg atttaccatt agatacagat ttaaataaac tgtcccacta cccatcagta
     3361 gcaattagta aagaggactt tcatgaagaa aaggctaatg aaactaatga gaatagctag
     3421 tcttaaatta aaattcactg ctcgttggag aatgtaatta aggagatatt cctaggaagt
     3481 taaaaataga tagacagatg ctttttattc ctaaataaga gtgaatgaaa atagataata
     3541 atcaagttca atatttcaat tttattaaaa gtacgaattt atcttctttg gagcgaaagg
     3601 gcttttgatt ttttatatag aataagttag gtgataaggt tgttaaaaaa tagattagtt
     3661 attttaggtt tgcttgttac ttgtgttttt ccattatatt cctgtaaaat tagtagtact
     3721 agaagtcaag aatataaatc ttcttctcaa aaaaacattt ttatttagga ggtatttatt
     3781 acgatttggc ttctggtgaa aaggctaaaa ttcagaggaa aaaaagcggc gcatggtcca
     3841 tgacttacaa aacactagac gggaatgttg ttgcgacttt tcgtccaatg tttaaaaaaa
     3901 caggttccca ttgctatatc tcaacttcaa aaatgacaaa atcagatggt aataacaatt
     3961 tttctatcca aatttattat aaaggtactt caaatgtgaa aatcaaaatg tttgatgata
     4021 acccagagca tactatgcag ttttcgaaga agaaatttgc taataatatg ttaaatagag
     4081 atgaatctac taccaaccat tcttatgata aggttttgga aggagattta accccattta
     4141 tgggtcaatt ttcaagtgat agttttaacc aaactattgt agattcgggt atgacttatg
     4201 ggggatatga accgaaagac tactatgagg ggcgaacaac agtttttcct gcaataacga
     4261 agacaggcta ttggaatggc ataacgtctc atggtagtta tgttataaaa aacgatgatt
     4321 tacccaagaa aattaaaaat tattatgaaa tacatgtgta tggcgctaat tctggagcga
     4381 taaatagtac aggtagtttg aaacgtatat tctatttagt tcccccaaaa gtggaggggc
     4441 cagatggaaa agtattatca aaaagacaag tttttgaagt aggagctaat aatcaattaa
     4501 tttctctttc ctatcaatct ccggattggt ggaaaagata taaaaaatct ggattagaaa
     4561 aagatttaga tgttgacgct atttatagag gtgacttttc ctcgttgaaa ggaacttgga
     4621 gaaatggtaa aggggaagtt ttaactatta atgatgatgg cagtacagat gatggtgaaa
     4681 gaattagtat cgggagtaca gaaaaaagta gactcccctc tgtagggatg agtagtggta
     4741 attttggggt atctgtttat ctcttccaaa ttggtgtatc gaatccttat atagagaacg
     4801 ggcatacatc ggacacgaca cgaccgcgct tagctattgg gcttaaccct ggagattttc
     4861 ctacagatgc ctattactat cgttactaaa tatcattcta tgaccttgat gtaacaataa
     4921 gctcttttac cactgtgact attaattttt aaagaataag aggatagaga aaaaatgtta
     4981 gaacgttatt cacgccctga gatggcggca atttggacag aagaaaataa atatcgtgct
     5041 tggttggagg ttgagatttt ggctgacgag gcatgggctg agttgggtga aattcctaag
     5101 gaggatgtga ctaagattcg tgagaaggcg gattttgaca ttgaccgcat tcttgagatt
     5161 gagcaggaca cgcgtcacga tgtggtggct ttcactcgtg cggtttctga gacgcttggt
     5221 gaggagcgca agtgggtgca ctacggtttg acgtcgactg acgtggtgga cactgcctac
     5281 ggttacctct acaagcaggc taacgacatt atccgtcgtg accttgagaa tttcacaaat
     5341 atcgtggctg agaaagctaa ggagcacaag ttcaccatca tgatgggtcg tacccacggt
     5401 gttcacgctg agccaacgac tttcggtctt aagttggcaa cttggtacag tgagatgaaa
     5461 cgtaatattg agcgttttga acatgctgcc gcaggagtgg aagctggtaa gatttcaggt
     5521 gctgttggta actttgccaa catcccacct tttgtggaag aatatgtctg tgacaaatta
     5581 ggcatccatc cgcaagaaat ttcaacacag gttcttccac gtgatctcca cgcagaatat
     5641 tttgcagtgc ttgcaagtat tgcaacatct atcgaacgta tggcgacaga gattcgtggt
     5701 ctgcaaaaat cagaacaacg tgaagttgaa gagttctttg ccaaaggtca gaaaggtagc
     5761 tctgctatgc ctcacaaacg caacccaatc ggttcagaga acatgacagg gctagcgcgc
     5821 gtaattcgtg gtcacatggt gacggcttat gagaacgtgg cactttggca cgagcgtgat
     5881 atttcgcact catcagctga gcgtatcatc acaccagaca caactatctt gattgactac
     5941 atgctcaacc gttttggcaa tatcgttaag aacttgactg tcttcccaga aaatatgatg
     6001 cgcaatatgg aatcaacttt tggtttgatt tatagtcaac gtgttatgct caaattgatt
     6061 gaaaaaggaa tgacacgaga agaagcttat gacttagttc aacctaagac agcttattcc
     6121 tgggacaatc aagtggattt caaaccactt ttagaagaag acaccaaagt tacctcttgt
     6181 cttacacagg aagaacttga tgaactattt aatccgattt attacacaaa acgtgttgat
     6241 gatatttttg aaaggttagg attagaaaaa taagaaaatt ccattactgg t
//
LOCUS       DBJYSH010000106         6211 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.106, whole genome shotgun sequence.
ACCESSION   DBJYSH010000106 DBJYSH010000000
VERSION     DBJYSH010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 6211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6211
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.106"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            213..500
                     /gene="rpsF"
                     /locus_tag="ACUBV3_001702"
     CDS             213..500
                     /gene="rpsF"
                     /locus_tag="ACUBV3_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HHU3848189.1"
                     /translation="MAKYEILYIIRPNIEEEAKNALVARFDSILSDNGATVVESKDWE
                     KRRLAYEIQDFTEGLYHIVNVEAEDAVALNEFDRLSKINGDILRHMIVKVD"
     gene            512..1003
                     /locus_tag="ACUBV3_001703"
     CDS             512..1003
                     /locus_tag="ACUBV3_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HHU3848190.1"
                     /translation="MINNVVLVGRMTRDAELRYTPSNQAVATFSLAVNRNFKNQSGER
                     EADFINCVIWRQQAENLANWAKKGALVGITGRIQTRNYENQQGQRVYVTEVVAESFQL
                     LESRATREGGSPNSYNNGGYNNAPSNNSYSASSQQTPNFSRDESPFGNSNPMDISDDD
                     LPF"
     gene            1048..1287
                     /gene="rpsR"
                     /locus_tag="ACUBV3_001704"
     CDS             1048..1287
                     /gene="rpsR"
                     /locus_tag="ACUBV3_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HHU3848191.1"
                     /translation="MAQQRRGGFKRRKKVDYIAANKIEYVDYKDTELLSRFVSERGKI
                     LPRRVTGTSAKNQRKVTTAIKRARVMALMPYVNED"
     gene            1457..2401
                     /locus_tag="ACUBV3_001705"
     CDS             1457..2401
                     /locus_tag="ACUBV3_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003085337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HHU3848192.1"
                     /translation="MKQMFLSTAIEFKEIETFEPGAWINLVNPSQEESEQVADQFGID
                     IDDLRAPLDVEETSRISVEDDYTLVIVDVPTYEERNNKSYYMTIPMGIIVTDNAVITT
                     CLEHLTLFDHFYRRRVKNFYTFMKTRFVFQLLYRNAELYLQALRTIDRQSDKIEAQLE
                     SATRNEQLIDMMELEKSIVYLKASLKFNERIVKKLTSSTSSLKKYIEDEDLLEDTLIE
                     TQQAIEMANIYENVLNAMTETTASIIGNNQNTIMKTLALVTMTLDIPTVIFSAYGMNF
                     QNNWMPLNGLAHGFIYVVLLAFLMSSFVVFYFIRKKWF"
     gene            2426..3097
                     /locus_tag="ACUBV3_001706"
     CDS             2426..3097
                     /locus_tag="ACUBV3_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HHU3848193.1"
                     /translation="MTLQDLTKKNQEFVHIATNQLLADGKSDAEIKAILEEHLPEIID
                     NQKKGITARSLLGAPTTWAASFTERPEDKARVSVQKNTNPWLMWLDTSLLFLGLVTAL
                     NGLMLLFGQSNVNTGLISILTLGFGGGAAMYVTYYYIYRHMGKPKSERPGWLKSFAVL
                     ALVMLVWFALFAVVPLLPATINPKLPEVVLFIIALASFGLRFYLQRKYNIQSSMAPVA
                     PQQRR"
     gene            3229..6057
                     /gene="uvrA"
                     /locus_tag="ACUBV3_001707"
     CDS             3229..6057
                     /gene="uvrA"
                     /locus_tag="ACUBV3_001707"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001932063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HHU3848194.1"
                     /translation="MQDKLMIRGARAHNLKNISVDIPRDKLVVVTGLSGSGKSSLAFD
                     TIYAEGQRRYVESLSAYARQFLGNMEKPDVDSIDGLSPAISIDQKTTSKNPRSTVGTV
                     TEINDYLRLLYARVGTPYCINGHGAITASSVEQIVDKVLALPERTKMQILAPIIRRKK
                     GQHKSTFEKIQKDGYVRVRIDGDIHDVTEVPELSKSKMHNIDIVVDRLINKEGIRSRL
                     FDSVEAALRLSDGYVVIDTMDGNELLFSEHYSCPECGFTVPELEPRLFSFNAPFGSCT
                     TCDGLGIKLEVDIDLVIPDKSKTLREGALVPWNPISSNYYPTMLEQAMTQFGVDMDTP
                     FEKLSKAEQDLALYGSGEREFHFHYINDFGGERNIDLPFEGVVNNINRRYHETNSDYT
                     RNVMREYMNELKCNTCHGYRLNDQALCVRVGGEEGLNIGQVSDLSIADHLELLETLRL
                     SSNEQLIARPIIKEIHDRLSFLNNVGLNYLNLSRSAGTLSGGESQRIRLATQIGSNLS
                     GVLYVLDEPSIGLHQRDNDRLIDSLKKMRDLGNTLIVVEHDEDTMMAADWLIDVGPGA
                     GAFGGEIVASGTPKQVAKNTKSITGQYLSGKKVIPVPSERRVGNGRFLEIKGAAENNL
                     QNLDVKFPLGKFIAVTGVSGSGKSTLINSILKKAVAQKLNRNSDKPGKYVSLEGIEYV
                     DRLIDIDQSPIGRTPRSNPATYTGVFDDIRDLFAQTNEAKIRGYKKGRFSFNVKGGRC
                     ESCSGDGIIKIEMHFLPDVYVPCEVCHGTRYNSETLEVHYKEKNIAQILDMTVNDAVT
                     FFAAIPKIARKLQTIKDVGLGYVTLGQPATTLSGGEAQRMKLASELHKRSTGKSLYIL
                     DEPTTGLHADDIARLLKVLDRFVDDGNTVLVIEHNLDVIKTADHIIDLGPEGGIGGGQ
                     IVAIGTPEEVAENPKSYTGYYLKEKLAR"
BASE COUNT         2003 a         1031 c         1240 g         1937 t
ORIGIN      
        1 ataagtcagt aaaagaaaat ggctacatag gtttgatata aggcacattt caataaagaa
       61 aaataaaaat gtttttaaat gatatcaggg aaataaaaac ataaaaacct tgcattatta
      121 acttatttct actataataa tatcttgtga gcataacctc acttactcat gaaaaattca
      181 tgagtcatta gaccaaaagg aggaacatat caatggctaa atacgaaatt ctttatatta
      241 ttcgtccaaa cattgaggaa gaagctaaaa acgctttggt agcacgtttt gattctatct
      301 tatctgacaa cggtgcaact gttgttgaat caaaagattg ggaaaaacgt cgtcttgcat
      361 acgaaatcca agatttcact gaaggacttt accatatcgt taacgttgaa gctgaagatg
      421 cagtagctct taatgagttt gaccgtcttt caaaaatcaa cggtgacatt cttcgtcaca
      481 tgatcgttaa agtagactaa gaaggtagtt tatgattaat aatgtagtac ttgtaggtcg
      541 catgacccgt gatgcagaac ttcgttatac accaagtaat caagcggtag ccactttttc
      601 acttgcagtt aatcgtaatt ttaaaaatca atctggcgaa cgtgaggctg attttattaa
      661 ctgtgttatt tggcgccaac aagctgaaaa cttggctaac tgggcaaaaa aaggtgcttt
      721 ggttggaatt acaggtcgta tccaaacacg taattatgaa aaccaacaag gtcaacgtgt
      781 ctatgtaaca gaagttgttg cggaaagttt ccaattattg gaaagtcgtg ctacacgaga
      841 aggtggttca cctaactctt ataataatgg tggctataac aatgctccgt caaataatag
      901 ttattcagct tcttctcaac aaacacctaa ttttagtcgt gatgagagtc cgtttggtaa
      961 ctcaaatcca atggatattt cagatgatga tctaccattc taatataata gatggacaga
     1021 atttaaaact ataaaggaga aataaacatg gctcaacaac gtcgtggcgg attcaaacgc
     1081 cgtaaaaaag ttgattatat cgcagctaac aagattgaat atgttgatta caaagataca
     1141 gagcttctta gccgtttcgt ttcagaacgt ggaaaaattt taccacgtcg tgtaacagga
     1201 acttcagcta aaaaccaacg taaagtaaca acagcaatca aacgtgctcg tgttatggct
     1261 cttatgcctt acgtaaatga agattaatat aaaatagccc tagcggctat ttttttattc
     1321 tttttaccat tattattggt atttacgagt caaataatag aaaaatttag gataccaccg
     1381 tattaaccgt gcaaaacaaa tggttttcct gtataatact aaggtatgga ttttttataa
     1441 caaaggagga atcgccatga agcaaatgtt tctttcaacg gcaattgaat ttaaagaaat
     1501 tgaaacattc gagccaggtg cttggataaa tctggttaat ccctcccagg aagaatccga
     1561 acaagtagct gatcaatttg gtattgatat tgatgattta cgtgctcccc tcgatgtgga
     1621 agaaacttcc cgtatctcgg ttgaagatga ttatacttta gttattgttg acgttcctac
     1681 ctatgaagaa cgcaataaca aaagttatta tatgactatt cctatgggca ttatcgttac
     1741 tgacaacgct gttattacta cttgcctaga acatttgacg ctgtttgatc acttttatag
     1801 acgacgtgtc aaaaattttt atacctttat gaagacacgt tttgttttcc aattgcttta
     1861 tcgcaatgcc gaattatact tacaggcttt gcgtactatt gataggcaga gcgataaaat
     1921 tgaagcacaa ctagagagtg caactcgaaa tgagcaattg atcgacatga tggaattgga
     1981 aaaatctatt gtctatctaa aggcctcttt aaaatttaat gagcgaatcg tcaaaaaatt
     2041 gaccagtagt accagtagtt taaaaaaata cattgaagat gaagatctgc ttgaagatac
     2101 attgatcgaa actcagcagg ctattgaaat ggcaaatatc tatgaaaatg tcttaaatgc
     2161 catgacagaa acgactgctt caatcatcgg taataatcaa aataccatta tgaaaacctt
     2221 agccctagta accatgacat tggatattcc aacggttatt ttttcggcgt atgggatgaa
     2281 ctttcaaaat aattggatgc ctttgaatgg tctcgcacat ggctttatct atgttgtcct
     2341 tttggccttt ttgatgagtt catttgttgt attttatttt attcgtaaaa aatggtttta
     2401 atagtatatt aatcaaggag ttcacatgac tttacaagac ttaactaaaa agaatcaaga
     2461 gtttgttcat attgcaacta atcaactttt agctgatgga aaatcagatg cagaaatcaa
     2521 agctatttta gaagaacatc tcccagagat tatagataat cagaaaaaag gaattaccgc
     2581 acgctcactc ttaggagcac caactacttg ggcagcctca ttcactgagc gacctgagga
     2641 taaagctaga gtatctgtac aaaaaaatac caatccttgg ttgatgtggt tagatacttc
     2701 cttactattt cttggcttag taactgcttt gaatggttta atgttgctat ttggtcaatc
     2761 taatgtcaat acagggctta tttcaatttt aactcttggt tttggtggtg gtgcggccat
     2821 gtacgttact tattactata tttatcgcca tatgggtaaa ccaaaatctg aaagaccagg
     2881 ctggcttaaa tctttcgcag tactggcact agttatgtta gtttggtttg ccttatttgc
     2941 agtagtacct cttctacctg caactattaa tcctaaactt cctgaggttg ttttgtttat
     3001 tattgcttta gcatcgtttg gtttgcgctt ttatttacaa cgtaaatata atattcaaag
     3061 ctcaatggca ccagtggcac cacaacaaag aagataataa caaagagtat gaacgtatta
     3121 gttcatctct tttttctatg aaatttaaaa ctaaaaaaat actgacttta ttaaaatttg
     3181 atataataag tcaacactat tcaaaaagga gtgctgaggc acaacattat gcaagataaa
     3241 ttaatgataa gaggagcaag agctcacaat ttaaaaaata taagtgtcga catcccacga
     3301 gacaagttag ttgtcgttac gggactatca ggatcaggta agtctagttt ggcctttgac
     3361 actatctatg cagaaggaca acgtcgttac gttgaaagtc tttctgctta cgcgcgccaa
     3421 tttttgggaa atatggaaaa gcctgatgta gattctattg atggccttag tccagctatt
     3481 tctattgatc agaaaacaac aagtaagaat cctcgctcta ctgttgggac tgtaacagaa
     3541 attaacgatt atttacgttt gctttatgct cgtgttggaa ccccatattg tatcaatggt
     3601 cacggagcca tcacagcatc ttctgtagag caaattgtgg ataaggtact agcgcttcca
     3661 gagcgaacaa agatgcaaat tttagcccca attatacgtc gaaaaaaagg acaacacaaa
     3721 tcaacatttg agaaaataca aaaagatggt tacgttcgtg ttcgtattga tggtgatatt
     3781 cacgatgtta ctgaggtgcc tgaattatca aaaagtaaga tgcacaatat tgatattgta
     3841 gtagatcgat taattaataa agagggcatt cgttcccgct tatttgattc cgttgaagcg
     3901 gctctacgtt tgtcggatgg ttatgtcgtt attgatacaa tggatggcaa tgagttattg
     3961 ttttcagagc attattcttg tcctgaatgt ggttttactg ttcctgaatt agagccacgc
     4021 cttttttctt ttaatgctcc ctttggttct tgtacgacct gtgatggctt aggaattaag
     4081 ttagaagtgg atatagattt ggttattcca gataaaagca agacccttag agaaggggcc
     4141 ttagttcctt ggaatccaat ttcatcaaat tattatccaa cgatgctaga gcaagctatg
     4201 actcaatttg gtgttgatat ggatacgcca tttgaaaaat taagcaaggc ggagcaggac
     4261 ctagccctat atggttcagg cgaacgagag ttccatttcc attatattaa cgattttgga
     4321 ggcgagagaa atattgattt gccttttgaa ggggttgtca ataatattaa tcgtcgttac
     4381 catgaaacta atagcgatta cacacgcaat gttatgcgag aatatatgaa cgagctaaaa
     4441 tgtaacactt gtcatggcta tcgtctcaat gatcaagcac tttgcgtaag agttggggga
     4501 gaagaaggtc ttaatattgg gcaagtctca gatttatcga tagcagacca tttagaactt
     4561 ttagaaacgt tgcgtttatc ttcaaatgaa caattaattg ctcgaccaat tatcaaagaa
     4621 attcatgacc gcctaagttt cttaaataat gttgggctta attatttaaa tctgtcacgt
     4681 tctgcaggaa cgctttccgg gggcgagagt caacgtattc gtttggctac tcaaattggg
     4741 tctaatttat caggtgtcct ttatgtactt gatgaaccgt ccattggact tcatcagcga
     4801 gacaatgacc gtttgattga tagtcttaag aaaatgcgtg atttgggtaa tacgcttatt
     4861 gttgttgaac atgatgaaga tacaatgatg gcagctgatt ggttaatcga tgtgggacct
     4921 ggcgctggtg cgtttggtgg tgagattgtg gcctctggca caccgaagca agtagctaaa
     4981 aatactaaat ctattacagg acaatatttg tcaggtaaga aagtgattcc tgtgccatct
     5041 gaacgaagag taggcaatgg tcgcttttta gaaatcaaag gtgcggcaga aaacaaccta
     5101 caaaatttag atgtcaagtt ccctctcggt aaatttattg ccgtaactgg tgtctcaggt
     5161 tctggaaaat caaccttaat taatagtatt ttgaaaaaag ctgttgcgca gaagcttaac
     5221 cgtaactcag ataaaccagg taaatatgtt tctttagaag gcattgaata tgttgatcga
     5281 ttgattgata ttgaccaaag tccaattggt cggaccccac gttctaatcc cgcaacctat
     5341 acaggtgttt ttgatgatat tcgtgatctt tttgctcaaa ctaatgaggc aaaaattcga
     5401 ggttataaaa aaggacgctt ctcatttaac gtcaaaggtg gacgttgtga gtcttgttct
     5461 ggtgatggta tcatcaaaat tgagatgcat tttttaccag atgtctacgt tccttgtgaa
     5521 gtatgccacg gcacgcgcta taatagtgag actctggaag tacattacaa agaaaaaaat
     5581 attgcacaaa ttcttgatat gacagtgaat gatgcagtta cattttttgc agctattcct
     5641 aaaattgctc gtaagttaca gactattaag gatgttggtt tgggatatgt cactttgggg
     5701 caaccagcta caaccttatc aggaggagaa gctcagcgaa tgaagttggc aagtgaatta
     5761 cataagcgtt cgacaggtaa gagtctttac atattagacg aaccaacaac ggggcttcat
     5821 gcagatgata ttgctcgctt acttaaggtt ttggatcgtt ttgtagatga tggtaataca
     5881 gtgcttgtta ttgagcataa ccttgatgtt attaaaactg ctgaccacat tattgatctc
     5941 ggccctgaag gaggtattgg tggcggacaa attgtagcta tcggaacacc agaagaagtt
     6001 gctgaaaatc ctaaatccta tacaggatat tatttaaaag agaagttagc aagatagttt
     6061 attgggagct gagtaatatt actcagttcc tttttgtaag ctaacattgc gtagaagtca
     6121 ttaaatggag aacgctttat taggaaaaat agtggggaga tattttagaa aaaggaaata
     6181 atgatgtttt gaaaaaaata gtttctatga g
//
LOCUS       DBJYSH010000107         6180 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.107, whole genome shotgun sequence.
ACCESSION   DBJYSH010000107 DBJYSH010000000
VERSION     DBJYSH010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 6180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6180
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.107"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            84..695
                     /locus_tag="ACUBV3_001708"
     CDS             84..695
                     /locus_tag="ACUBV3_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HHU3848195.1"
                     /translation="MTLDSHSSDDGYTIRSLYFDSLEDVDWQEKEDGVEIRRKIRLRN
                     YGSQSNFAKLEMKQKQGANQKKRSLKLSKTDAQALINGNNSVLLNYPDTFAAECYSRI
                     TTQLYQPKTVIEYQRKAFVAKENNIRITFDHHIIGTEASYDIFSEDLNQNYLLDPYLV
                     ILEVKYNGFLLSYIKELLDSVDRSELSASKYCLGRAVSKHYRF"
     gene            747..1424
                     /locus_tag="ACUBV3_001709"
     CDS             747..1424
                     /locus_tag="ACUBV3_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HHU3848196.1"
                     /translation="MKDLLRNSLEQSGNLSFQDMILHILVAALLSVVIYVSYAYTHSG
                     TAYSKKFNVSLMTLTVLTATVMTVIGNNVALSLGMVGALSVVRFRTAIKDSRDTVYIF
                     WTIVVGICCGVGDYVVAALGSSVIFILLWVMGRVKNENRMLLIVKCDRTLEVDLEGIF
                     FQYFDGKAVQRVKNSTTNTIEMIFEISRKDYDKQLHVDNQLTEKVYQLGNIDYFNIVS
                     QSDEING"
     gene            1417..2862
                     /locus_tag="ACUBV3_001710"
     CDS             1417..2862
                     /locus_tag="ACUBV3_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HHU3848197.1"
                     /translation="MGRLMRGKVIYGTTLIGLFLFLFFYFWIPKHHIERIHHHRIKQV
                     DAKSDLTGFKTHLPIISIDTKQQVIPLVTKEGGKYVKARDNINVDIELRDSPSRSHHL
                     SEKPRIRTKGLISYRGNSSRYFDKKSLKVKFVTNKLKEKKHRLAGMPKESEWVLHGPF
                     LDRTLLRNYLSYNIAGEIMSYAPNVRYCELFVNGEYQGVYLAVENIEQGEQRVPIEKS
                     DKKLHKTPYIVAWDREHKAKQKLDNYVHYTHQSGISALDVKYPGKQRLTSKQLEFINK
                     DINHIEKVLYSYDFSQYPKYIDRESFANYFVINEFFRNVDAGKFSTYLYKDLRDRAKL
                     VVWDFNNAFDNQIEGRVDEADFTLTDAPWFNMLIKDKAFIDLVVHRYKELRKGVLATE
                     YLSNYIDETRHFLGPAIDRNYKKWGYVFDLKNTDPRNYLIPTERNVTSYHKSVEQLKD
                     FIKKRGRWMDRNIETLYQYSAPSKNTNTLLE"
     gene            2869..5028
                     /locus_tag="ACUBV3_001711"
     CDS             2869..5028
                     /locus_tag="ACUBV3_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000760424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3848198.1"
                     /translation="MKKYLSIVALLTILFSSIYYLYYFDGSLYLPKGLLKENTRTNFV
                     VKGDTVLHKPTNKPFVVKGVDVESSLAGYHHNDFPITQKTYREWFHLISNMGANTVRV
                     KVPMNVAFYDALYHHNKASKRPLYLLQGIRIDSYRNNASITAFNDNYRGYLKREAKGV
                     VDILHGRKQVWNTDFGSRHYHYDLSPWVLGYVVGDDWNSGTVAYTNHQEKKTQYKGRY
                     FKTSVAANPFEVMLAQVMDELTHYETAKYGWQHLISFSNSPTTDPFHYRKPFEAQAPK
                     YVQLNVENIQANSNVKAGMFAAYKAIDFHPRYKDYLLFDKENISKEDRQKIKELSLSQ
                     GYVKLLNAYHKIPVLVTGYGYSTARGIAQKEIDKRPLPINEKEQGQRLLEDYESFISS
                     GSFGATINAWQDDWNARAWNTSFATNKHSQFLWGDAQVFNQGYGLLGFKNAKHHYQVD
                     GKRGKGEWKHPLMTSATGDDLYASSDESYLYLAIKTKPEKLKEKRLLPIDITPKSGSR
                     KMNGSKVTFSKSSDFVLSIDPNGKSELFVQERYNALKANYLRQLNGKDFYAFPPKKNS
                     SNFEQINMVLRNTKIVEDMEKVKATERFLPTHPTGLLKTGTTDRHQKTFDSQTDISFG
                     KDFIEVRIPWQLLNFSDPSSQKIHDDYFKHYGVKELEIESIALGLGANSKENTLIKMA
                     DYRLKNWERPDTKTFLKDSYYSIKKEWSKERERTYGT"
     gene            5168..6121
                     /locus_tag="ACUBV3_001712"
                     /pseudo
     CDS             5168..6121
                     /locus_tag="ACUBV3_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643060.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
BASE COUNT         2121 a          900 c         1206 g         1953 t
ORIGIN      
        1 atgaacgaag ttcttagaca ggaaaaaaat atttaatatc acttgaagat tactatcttt
       61 tatctaagaa attttcccaa gtaatgacgc tcgattcgca tagttcagac gacggctata
      121 ctatccgttc gctatacttt gattctttag aagatgttga ttggcaagaa aaggaagatg
      181 gtgttgagat acgaagaaag attcgtttgc gcaattatgg tagtcaaagc aattttgcga
      241 aattagaaat gaaacaaaaa caaggtgcta atcaaaaaaa gcgctctcta aaattaagca
      301 agacagatgc tcaagcgctg attaatggta acaatagtgt tttattaaat tatccggata
      361 catttgctgc ggagtgttat tcaaggataa caacgcaact ttatcaacct aaaacggtta
      421 ttgagtacca aagaaaagct tttgttgcta aggaaaacaa tatccgaatc acctttgacc
      481 accatataat aggtacagag gctagttatg atattttctc agaggattta aatcaaaatt
      541 atttattaga tccttatctt gttattttag aagttaaata taatggattt ttattgtctt
      601 atattaaaga actcttagat tcggtagata gaagtgaact ttcagcgagt aagtattgtt
      661 taggaagagc tgttagtaaa cattatcgtt tttagtgaaa aagtatatac gttaattatt
      721 attaaatgtg agaggataac aaaaaaatga aagacctatt acgaaatagt ctagagcaaa
      781 gtggaaattt aagttttcaa gatatgattt tacatattct tgtagcagct ttattgagtg
      841 tagttattta tgtttcctat gcttatacgc atagtggaac tgcctatagt aaaaagttta
      901 atgtttcatt aatgacattg acggtcttga ctgcaacagt aatgaccgtt attggtaata
      961 atgtagcctt gtcattgggt atggtcggtg ccttgtcagt tgttcgtttt aggacagcca
     1021 taaaagattc aagagataca gtttatattt tttggaccat agttgttggt atctgttgtg
     1081 gtgtcggtga ctatgtggta gctgcattag gaagtagcgt tatctttatc ttattatggg
     1141 ttatgggacg tgttaaaaac gagaatcgta tgttattgat tgtgaagtgc gatagaacac
     1201 tagaagttga tttagaagga attttcttcc aatattttga cggaaaagct gttcagcgtg
     1261 ttaaaaattc aacaactaat actattgaaa tgattttcga aatctctaga aaagattacg
     1321 ataagcaact ccatgtagat aatcagttaa ctgaaaaagt gtaccaattg ggaaatattg
     1381 attatttcaa cattgttagc caaagcgacg aaatcaatgg gtagacttat gagagggaag
     1441 gttatttacg gcacaaccct tataggtctt tttctattct tatttttcta tttttggatt
     1501 cctaagcatc acatcgagag aatacatcat catcgtataa agcaggtaga tgcgaagagt
     1561 gatttaacag gatttaaaac ccatttgccc attatcagca ttgatacaaa gcaacaagtt
     1621 attcctcttg ttacaaaaga aggcggaaaa tatgtcaaag ctagggataa tattaatgtt
     1681 gatatcgaat tacgggattc tccaagtaga tcacatcatt tatcagaaaa gccgagaatt
     1741 aggacaaaag ggttaatatc atatagagga aattcctctc gttactttga taagaagtca
     1801 ttgaaagtta agtttgttac taataagtta aaggaaaaga agcatcgatt agcaggaatg
     1861 cctaaagaat cggagtgggt attgcatggt ccctttctag acagaacatt attaagaaat
     1921 tatctgagtt ataatattgc tggtgagatt atgtcctatg ccccaaacgt tcgctactgt
     1981 gagttatttg tcaatggtga gtatcaggga gtatatctag cagttgagaa tatcgaacaa
     2041 ggtgagcaac gagttcccat tgaaaaaagt gataaaaagt tgcataaaac accttatatc
     2101 gttgcttggg atcgtgaaca taaggcaaag caaaagttag ataactatgt acattataca
     2161 caccagtctg gtatctcagc tcttgatgtg aaatatccag gtaaacaaag attgacatca
     2221 aaacaattag agtttattaa taaggacatc aatcatattg aaaaagtcct ttattcttat
     2281 gatttttcac aatatcctaa atatattgat agagaatcat ttgcaaatta ttttgtcatc
     2341 aatgaatttt ttagaaatgt ggatgcgggt aaattttcaa cctatctata taaagatctt
     2401 cgtgatagag caaaactagt agtttgggat tttaataatg cttttgataa tcaaattgaa
     2461 ggacgtgttg atgaggcaga ttttaccttg acggatgcac cgtggttcaa tatgctcatt
     2521 aaggacaaag catttataga cctggttgtt catcgctata aagaattacg aaagggagtt
     2581 ctagcgacag aatatttatc taattacata gacgagacaa ggcatttttt aggtccagct
     2641 attgatcgaa attataaaaa atggggctat gtctttgatt taaagaatac tgatccaagg
     2701 aattatttga ttcctactga acgcaatgtg acaagttatc acaaatcagt agaacaatta
     2761 aaagatttta tcaaaaagcg tggcagatgg atggatagaa atattgagac cttgtatcaa
     2821 tatagtgcac catcgaaaaa tactaatact ttactggagt agaacattat gaaaaaatat
     2881 ctaagtattg tagccctttt aactattttg tttagctcta tttattattt gtattacttt
     2941 gatggtagtt tgtatttacc aaagggctta ttaaaagaaa atacaagaac taactttgtt
     3001 gttaaaggtg atactgtact tcacaagccc accaataaac cttttgttgt taaaggagta
     3061 gacgttgagt cttccttagc gggttatcat cacaacgatt ttcctattac tcaaaaaacg
     3121 tatcgtgaat ggttccattt aatttccaac atgggggcaa atactgtaag agtcaaggta
     3181 ccgatgaatg ttgcatttta cgatgcctta tatcaccaca acaaagcatc aaagaggcca
     3241 ctgtatttgt tgcaaggaat acgtatagat tcttatcgca ataatgcttc tataacagct
     3301 tttaatgata attatagggg gtatttaaaa cgagaagcaa aaggcgttgt ggatattctc
     3361 catgggcgta agcaagtatg gaatactgat tttggtagcc gtcattatca ttatgatctt
     3421 agtccttggg tacttggtta tgtcgtaggg gatgattgga atagtggtac tgtcgcttat
     3481 actaatcatc aagagaaaaa aacgcaatat aaaggacgtt attttaaaac ttctgtggca
     3541 gctaatccat ttgaggtcat gctagctcaa gtaatggatg aattgacaca ttatgagaca
     3601 gctaaatatg gttggcaaca tttgattagt ttttcaaact caccaacaac agaccctttt
     3661 cattatcgaa aaccatttga ggcacaggct cctaaatacg tacaactaaa tgtagaaaat
     3721 attcaagcta attcaaatgt taaagcaggt atgtttgcag catataaagc tattgatttc
     3781 catcctcgat acaaggatta tctattattt gataaagaga atatcagtaa agaagataga
     3841 caaaagatta aagaactttc tttgtcacag ggatacgtta aactgctaaa tgcttatcac
     3901 aaaatccctg ttctagtcac gggttatggc tattcgacag cgagaggtat tgcccaaaaa
     3961 gaaattgata aacgtcctct gccgattaat gaaaaagaac aaggtcagcg tttactagaa
     4021 gattatgaat cttttatatc atccggtagt tttggagcga ctatcaatgc atggcaagac
     4081 gattggaatg caagggcgtg gaatacatct ttcgccacaa ataaacatag tcaattccta
     4141 tggggggatg cacaagtatt taatcaaggt tatggtttat taggctttaa aaacgcaaaa
     4201 catcattatc aagttgatgg taaaagaggc aaaggagagt ggaaacatcc tctgatgact
     4261 agtgcaacag gagatgactt atatgctagc agtgatgaaa gctatctcta ccttgcgatt
     4321 aaaacaaaac ctgaaaaact aaaagaaaaa cgattattac caatagatat tacaccaaaa
     4381 tctggtagta gaaaaatgaa tggtagtaag gtcacatttt ctaaatctag tgactttgta
     4441 ttgtctattg atccaaatgg caagtctgaa ttatttgtcc aagagcgcta taatgcctta
     4501 aaagcgaact atcttcgaca gcttaacggt aaagattttt atgctttccc accaaagaag
     4561 aacagtagta attttgagca gataaatatg gtattgagaa atacaaagat tgttgaagac
     4621 atggaaaaag taaaagcaac agagaggttc ttaccaactc atcctactgg tcttctcaaa
     4681 acaggaacaa ctgataggca ccaaaaaaca tttgattcac aaacagatat ttcgtttgga
     4741 aaggacttta tagaggtcag aattccgtgg cagttgttga atttttctga tccatcatct
     4801 caaaaaattc acgatgatta ctttaaacat tatggtgtga aggagttaga aattgagagc
     4861 attgctttag gattaggtgc taatagcaaa gaaaacacac tgataaagat ggcagattat
     4921 cgtttgaaaa attgggagag acccgatacc aaaacctttt taaaagactc ctattatagt
     4981 attaagaaag aatggtctaa agaaagagag agaacatatg gtacataata ttttatattt
     5041 ttacattttg atttgcttag caattattgt ttttaacatt cactatatct taagacaacg
     5101 attagataag cttagttggc ttcgtattcg gaaaaaggat ataaatatca aataaagggg
     5161 attttctatg gcattatttt ttaccttcct aaaggtatct caagtcattt tcttagtatt
     5221 attgattgcc tatgccagct ttttagtgat ttcattagtc attggaggtg ttcatctcta
     5281 tgaaaaaaat gagcgcaaaa aaagtttaac tagaaataaa gaaaaaattc caatttcaat
     5341 tattgtacca gcctataatg aatcaacaac cattgtgagt tcgattgatt cgttattgca
     5401 ccttgattat gaagcgtatg aaattatcgt tgtggatgac ggttccagtg acaatacttc
     5461 agatgttctc aaggaagagt ttgcattaat gaaaatctcg aatacgattg attcaattat
     5521 tgctactcaa acttgtaaag atgtctttca gagacaggtt ggtaaggtta aactgacact
     5581 cattgttaaa gaaaatggtg gcaaagggga tgccttaaat atggggatca atgctgctaa
     5641 ttacgattat tttctatgtc tagatgcaga ttctatgttg caggtagatt ctttgagtca
     5701 aattagcaaa tccatacagg tagatccgac ggttatagca gtgggagggc tcgtccaagt
     5761 tgcccaaggt gttaagattg agcaggggaa agtagcatct tatcgtttgc cttggagaat
     5821 tattccttgt gcacaagcac ttgagtatga ttcatcattt ttaggagctc gaatcttttt
     5881 agactattta agagctaatt taattatttc gggggctttc ggtcttttta agaaagacct
     5941 tgttaaagcg gtgggaggat atgacaccca gactctagga gaagatatgg agttggtgat
     6001 gaaattacat ttcttctgtc gtaataataa tattccttat cgtatttgtt atgaaacaga
     6061 tgctgtttgt tggagtcaag caccaacgaa tttaggggat ttacgtaaac agagacgccg
     6121 gtgatatctt ggcttgtacc aatgtttaaa aaaatataaa agtatttttg ccaattatcg
//
LOCUS       DBJYSH010000108         5981 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.108, whole genome shotgun sequence.
ACCESSION   DBJYSH010000108 DBJYSH010000000
VERSION     DBJYSH010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 5981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5981
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.108"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            complement(23..1927)
                     /locus_tag="ACUBV3_001713"
     CDS             complement(23..1927)
                     /locus_tag="ACUBV3_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000654956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III domain-containing protein"
                     /protein_id="HHU3848199.1"
                     /translation="MKDRLYNKFKDFDREFCQKYIKTYQSNAYQEMKASVNLMMRNTF
                     VFNDNWDMEPCSKAYCLDPLEWDKPVTDDPEWLYMLNRQTYLFKFLVVYIVEGDKSYL
                     RQMKYFMYHWIDCQFTLKPEGAVSRTIDTGIRCMSWLKVLIFLDYFGLITETKKIKLL
                     TSLREQITYMRDYYREKDSLSNWGILQTTAILACLYYYEDELNLPEIQSFAEEELLLQ
                     IKLQILDDGSQYEQSIMYHVEVLKSLMELVILAPKYYLPLEETIEKMVTYLIAMTGPD
                     YCQLAIGDSDVTDTRDILTLATLVLKSSKTKSFSFDNVNLETLLLFGKPSIYLFEEIP
                     RATIGESAYLFPDSGHVCLRDDRRYIFFKNGPFGSAHTHSDNNSVCLYDKKKPIFIDA
                     GRYTYKEEQLRYDFKRSTSHSTCTLDGQPLEMIKDSWTYNSYPKCDYCQLTSKDRYHL
                     VEGQLHVQRASDIYYHKRWLLTLPQAITLVIDKVSCPGEHVLTNQYILDDQVIYENGF
                     VNDLKLVSPTTFNLEDCLISKRYNQLTESHKLVKKIKFVDEVMDYTLIVDRNCQVKCV
                     PLVQTNSHKELSNSIAFDIRSQDFHYLIGVLMDDIIFGDKLYLMQGIKCKGKVIVYDK
                     NNGKMSRLKN"
     gene            complement(2007..2822)
                     /locus_tag="ACUBV3_001714"
     CDS             complement(2007..2822)
                     /locus_tag="ACUBV3_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HHU3848200.1"
                     /translation="MTGSKKLAKSDYTKTALRAFYLQNGFNYSNYQGLGYANVIYPAL
                     KKYYGDDKKALAGALEENVEFYNTNPHFLPFVTSLHLAMLDNERPEEEIRGIKMALMG
                     PLAGIGDSLSQFCLAPLFSTIAASLATDGLVMGPILFFVAMNTILTGIKLVTGMYGYR
                     LGTSFIDKLSEQMSVISRAANIVGVTVISSLAATQVKLTIPYTFAPEKVTSTTQKIVT
                     VQGMLDKIAPALLPALYTFLMFYLIKNKKWTTYKLVILTVIIGILGSWLGILA"
     gene            complement(2809..3675)
                     /locus_tag="ACUBV3_001715"
     CDS             complement(2809..3675)
                     /locus_tag="ACUBV3_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HHU3848201.1"
                     /translation="MDISILQAVLIGLWTAFCFSGMLLGLYTNRCIVLSLGVGVILGD
                     IQTALAVGAISELAYMGFGVGAGGTVPPNPIGPGIFGTLMAITTAGTKGKITPEAALA
                     LSTPIAVGIQFLQTATYTAFAGAPETAKKALQAGNFRGFKIAANGTIWAFAGLGFGLG
                     VLGALSTQTLTDLFALIPPVLLNGLTLAGKMLPAIGFAMILSVMAKKELIPYILLGYV
                     LAVYFGLPVLTPTANGDGVLTSVATNSVLGVPTIGVAIIATIFALLDIFRKPAAPTKE
                     TKTEGDNQDDWI"
     gene            complement(3711..4205)
                     /locus_tag="ACUBV3_001716"
     CDS             complement(3711..4205)
                     /locus_tag="ACUBV3_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HHU3848202.1"
                     /translation="MAAGPNIVMTRVDERLIHGQGQLWVKFLSCNTVIVANDDVSKDH
                     LQQTLMKTVVPESIALRFFDIQKVIDIIHKANPAQTIFIIVKDLKDVYRLVAGGVPIK
                     EINIGNIHNGEGKEQVSRSIFLGMKDKEIIRKLNQEYHIAFNTKTTPTGNDGAVEVNI
                     LDYI"
     gene            complement(4260..5456)
                     /locus_tag="ACUBV3_001717"
     CDS             complement(4260..5456)
                     /locus_tag="ACUBV3_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HHU3848203.1"
                     /translation="MMKIKPVKVESIENPKRFLNSRLLTKIEVEEAIEKALKQLYINI
                     DYFGEEYPTPATFNNIYKVMDNTEWTNGFWTGCLWLAYEYNQDKKLKNIAHKNVLSFL
                     NRINNRIALDHHDLGFLYTPSCTAEYRINGDVKALEATIKAADKLMERYQEKGGFIQA
                     WGELGYKEHYRLIIDCLLNIQLLFFAYEQTGDEKYRQVAVNHFYASANNVVRDDSSAF
                     HTFYFDPETGEPLKGVTRQGYSDESSWARGQAWGIYGIPLSYRKMKDYQQIILFKGMT
                     NYFLNRLPEDKVSYWDLIFTDGSGQPRDTSATATAVCGIHEMLKYLPEVDPDKETYKY
                     AMHTMLRSLIEQYSNNELIAGRPLLLHGVYSWHSGKGVDEGNIWGDYYYLEALIRFYK
                     DWELYW"
     gene            complement(5459..5893)
                     /locus_tag="ACUBV3_001718"
     CDS             complement(5459..5893)
                     /locus_tag="ACUBV3_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HHU3848204.1"
                     /translation="MIKIIIVAHGNFPDGILSSLELIAGHQEYVVGINFIAGMSSNDV
                     RVALQREVIDFKEILVLTDLLGGTPFNVSSALSVEYTDKKIKVLSGLNLSMLMEAVLS
                     RTMFEHVDDLVDKVITSSHEGIVDFSTCLATQTAEATFEGGI"
BASE COUNT         1923 a         1196 c          913 g         1949 t
ORIGIN      
        1 gtatagcata ataatagccc tcttaatttt ttaaacgact cattttgcca ttatttttat
       61 catatacaat gacttttcct ttgcatttta tcccttgcat caaatagagt ttatccccaa
      121 agataatatc atccataagc actccaatta aataatgaaa gtcttgagac ctaatatcaa
      181 atgcaatgct attacttagt tccttatgac tgttcgtttg cactaaagga acacatttca
      241 cctgacagtt ccgatcaact atcaaggtat aatccatcac ctcatcaaca aattttattt
      301 tcttaactaa tttatgactt tctgtcaatt gattataccg cttagaaata aggcaatctt
      361 ctagattaaa ggtcgtagga cttactaatt tcaagtcatt aacaaaccca ttttcataaa
      421 tgacctgatc atctaaaata tattgatttg ttaagacatg ctctcctgga caactcacct
      481 tatcaataac taaggtaatg gcctgcggta aagttaacaa ccatcgctta tggtaataga
      541 tatcagaagc tctttggaca tgtagttgtc cttcgactaa atggtaccta tcctttgaag
      601 tcaactgaca atagtcacat tttggataag aattgtatgt ccaagagtcc ttgatcattt
      661 ctaagggttg cccatcaagg gtacatgttg aatgactagt cgaacgttta aaatcatacc
      721 ttagttgttc ttctttgtaa gtatatcttc ctgcatcaat gaaaataggt ttctttttat
      781 catagagaca aacactatta ttatcactat gagtatgagc gctaccaaat ggaccgttct
      841 taaaaaatat ataacgccta tcatcacgta aacacacatg accagaatct ggaaaaagat
      901 aagcagactc tcctattgtc gcacgcggta tttcttcaaa aagataaatt gatggcttcc
      961 caaacaaaag taaagtttct aaattaacat tatcaaaaga aaatgatttt gttttagaag
     1021 atttcaacac aagtgttgcc aaagttagaa tatcacgagt atcggtcaca tcactgtccc
     1081 ctatagctag ttgacaataa tccggaccag tcatagcaat tagataggta accatttttt
     1141 caatagtttc ttctaatggt agataatatt taggcgcaag gataactagt tccattaagg
     1201 acttcaagac ttctacatga tacataattg attgttcata ctggctcccg tcatctaaaa
     1261 tttgaagctt gatttgaagt aataattctt cttcagcaaa actctgaatt tcaggtagat
     1321 ttaattcatc ttcataatag tataaacacg ccaatattgc tgttgtttgt aaaattcccc
     1381 agttacttag actatctttt tcacgataat agtccctcat ataagttatc tgctcccgta
     1441 aactagttaa tagtttaatt tttttagttt ctgttattaa tccaaaataa tctaaaaaaa
     1501 tcaaaacttt gagccatgac atgcaacgta tcccagtgtc aatagtacga cttaccgccc
     1561 cttctggctt tagtgtaaat tgacaatcaa tccaatggta cataaaatat ttcatttgtc
     1621 taaggtaaga cttatcgcct tcaacaatgt agaccaccag gaatttaaaa agataagttt
     1681 gacgatttag catatacaac cattctggat catctgttac tggcttatcc cactccaaag
     1741 gatcaagaca atatgcttta gaacaaggtt ccatatccca attatcatta aatacaaaag
     1801 tgttcctcat catcaaatta acagaggctt tcatttcttg ataggcatta gattggtatg
     1861 ttttaatata cttttgacaa aattccctat caaaatcctt gaatttatta tataagcgat
     1921 ctttcatttt tttacaaaac gtcctcgtcc gatataaaaa ggactatgtc attgatatgt
     1981 cataatcctt atttatcaaa attttattaa gctaaaatac ctaaccagct accaaggata
     2041 ccaataataa ctgtaaggat aactaactta taagttgtcc attttttatt tttaataagg
     2101 taaaacataa ggaaagtgta gagtgctgga agtagagctg gtgcaatttt atcaagcata
     2161 ccttggacag taacaatctt ttgcgttgta ctagttactt tttctggagc aaaagtgtaa
     2221 gggattgtaa gcttaacttg tgttgcagct aaacttgaga tgaccgttac accaacgata
     2281 ttagctgccc gtgagataac tgacatttgt tcactcaatt tatcaataaa actggtccca
     2341 agacgataac catacatgcc agtaactaat ttaattcccg ttaaaatagt attcatagcc
     2401 acaaagaaca gaatcggtcc catcaccaaa ccatctgtcg ctaaagaggc tgcaatcgtt
     2461 gagaacaagg gtgctaagca aaattgtgat aaagaatcac cgatacctgc aagaggcccc
     2521 ataagcgcca tcttaatacc gcgaatttct tcttctggcc gctcattatc aagcattgct
     2581 aaatgtaagc tagttacaaa aggtaagaaa tgagggtttg tattgtaaaa ctccacattt
     2641 tcttctagag ctcccgctaa agcttttttg tcatctccat aatatttttt gagagctgga
     2701 tatatgacat tagcataacc aagaccttga tagttactgt aattaaagcc attttgtaaa
     2761 taaaaggcac gtaaagctgt tttagtgtaa tcactttttg ctaatttttt agatccagtc
     2821 atcttggttg tctccttctg tcttagtttc tttagtaggt gcagctggtt ttctgaaaat
     2881 atcaagcaaa gcaaatattg ttgcaattat agctaccccg atagttggaa ccccaagcac
     2941 actgttagta gccactgatg ttaaaactcc gtctccattt gctgttggag ttagaactgg
     3001 caaaccaaaa taaacagcta gaacatatcc aagtaggata taaggaatga gttctttttt
     3061 agccattaca gagagaatca tcgcaaaacc aattgcagga agcattttcc cagctaaggt
     3121 tagaccatta agcaaaacag ggggaatgag agcaaataaa tctgtcaaag tttgcgttga
     3181 aagagcaccc agtactccta aaccaaatcc caatccagcg aatgcccaaa ttgtaccatt
     3241 tgctgcaatt ttaaatcctc ggaaattacc tgcttgcaaa gcttttttag ctgtttcagg
     3301 cgcccctgca aaagcagtat aagttgctgt ttgaagaaat tgaataccaa ctgcaattgg
     3361 ggttgataat gcaagagcag cttccggtgt gatttttcct ttagttccag ctgttgtaat
     3421 agccatcaaa gttccaaaaa tcccaggacc gattgggttt ggaggaactg tccctcccgc
     3481 tcccacacca aatcccatgt aagccagttc agatattgct ccaactgcca aagctgtttg
     3541 aatatctcct aaaataacac caacgcctag agagagaacg atacaacgat ttgtatagag
     3601 gccaagcaac atgccactaa aacagaaagc cgtccacagt ccaattaaga cagcctgaag
     3661 aatactaata tccatatttt tctcctttat atgatagtaa gtttgtccaa ctaaatataa
     3721 tctaaaatat taacttccac agcaccatcg ttacctgttg gagttgtttt agtattaaat
     3781 gcaatgtgat attcttggtt taattttcta attatctctt tatccttcat ccctaagaag
     3841 atagagcgac ttacctgctc tttaccttct ccattatgaa tatttccaat attaatctct
     3901 ttaattggga cgccccctgc tacaagtcta tagacatctt tcaaatcttt aacaattata
     3961 aaaatcgtct gtgctgggtt ggccttgtga ataatatcaa taactttttg aatatcaaaa
     4021 aatcgcaatg ctattgattc tggcaccacc gtcttcatta acgtttgttg aagatgatct
     4081 ttggagacat catcatttgc cacaataaca gtattacaac ttaaaaattt cacccaaagt
     4141 tgtccttgtc catgaataag acgctcatca acacgtgtca ttacaatatt tggtcctgct
     4201 gccatttctg acctcctcgt tttctattat ttattgttta ttttgttagt aaaatactct
     4261 taccaataaa gttcccagtc tttatagaat cttattaagg cttctaagta ataataatct
     4321 ccccaaatat taccttcatc tactccttta cctgaatgcc acgaatacac accgtgcaat
     4381 agaagaggac gtcctgctat aagttcatta ttactatact gttcaatcag actacgaagc
     4441 attgtatgca tagcatattt gtatgtctct ttatcaggat ctacttctgg taaatattta
     4501 agcatctcat gaattccaca cacagccgtt gctgttgcgg atgtatctct aggctggccc
     4561 gagccatccg taaaaataag gtcccaatag gataccttgt cttcaggtaa acgatttaga
     4621 aaatagtttg tcataccttt aaaaaggata atctgctgat aatctttcat tttccggtaa
     4681 ctaagcggaa taccgtagat gccccatgct tgccctcttg cccaagatga ctcatcacta
     4741 taaccctgtc gtgtgacacc ttttaacggt tctccagttt ctgggtcgaa ataaaaagta
     4801 tgaaaagcag aagaatcatc acgcaccaca ttgttagctg aagcgtagaa gtgattcacc
     4861 gcaacttgtc tatacttttc atcacctgtc tgttcataag caaaaaataa gagttggata
     4921 ttaagtaagc aatcgataat taagcgatag tgttccttat acccgagttc tccccaagcc
     4981 tgaataaatc cacctttttc ttgatagcgc tccatcaatt tatctgcagc ttttatagtg
     5041 gcttctaaag ctttaacatc accattgata cgatattctg ctgtacaaga tggtgtgtaa
     5101 agaaatccta agtcgtggtg atctaatgct atacgattat taatacgatt tagaaatgac
     5161 aatacatttt tgtgggctat gttttttaac tttttatcct gattatactc ataagctaac
     5221 cacaagcacc ctgtccaaaa accatttgtc cattctgtgt tatccataac tttataaata
     5281 ttattgaatg ttgcaggcgt tggatactct tcaccaaagt aatcaatatt aatataaagt
     5341 tgcttcaagg ctttttcaat cgcttcctca acttcaatct tagttaataa tctactgttt
     5401 aaaaaacgct ttggattttc aattgactca accttgaccg gttttatttt catcattatt
     5461 aaatgcctcc ctcaaaagtt gcttctgcag tttgtgtagc aaggcaagta gaaaaatcaa
     5521 caattccttc atgagatgat gttataactt tatcaacaag atcatctaca tgttcaaaca
     5581 tcgttctaga taaaacagct tccattaaca ttgaaaggtt caagcccgat aaaactttta
     5641 tctttttatc tgtatattct actgataagg cggaagaaac attgaaagga gtcccaccta
     5701 gcaaatcagt caatactaaa atttctttga aatcgataac ctctctttgc aaagcaactc
     5761 taacatcatt agaagacatg cctgcaataa agttaatacc aacaacatat tcctgatgcc
     5821 cagcaataag ttctaaagag cttaaaatac catctggaaa gttgccgtgt gctacaataa
     5881 taatttttat catattttct cctcttgagt ttaccggttt acttttataa tcagattata
     5941 gcgctttcat ttttaggtgt caacatcttt tttatatttt t
//
LOCUS       DBJYSH010000109         5910 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.109, whole genome shotgun sequence.
ACCESSION   DBJYSH010000109 DBJYSH010000000
VERSION     DBJYSH010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 5910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5910
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.109"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            complement(42..215)
                     /gene="secE"
                     /locus_tag="ACUBV3_001719"
     CDS             complement(42..215)
                     /gene="secE"
                     /locus_tag="ACUBV3_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HHU3848205.1"
                     /translation="MFVKGIFQVLRDTTWPNRKQRWKDFISILEYTVFFTIVIYIFDK
                     LLAAGVMDLINRF"
     gene            complement(251..403)
                     /gene="rpmG"
                     /locus_tag="ACUBV3_001720"
     CDS             complement(251..403)
                     /gene="rpmG"
                     /locus_tag="ACUBV3_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HHU3848206.1"
                     /translation="MAQKKASLACTECGSRNYSIGVSSTPKPTRLEVNKFCKYCKKYT
                     IHKETR"
     gene            complement(452..2773)
                     /gene="pbp2a"
                     /locus_tag="ACUBV3_001721"
     CDS             complement(452..2773)
                     /gene="pbp2a"
                     /locus_tag="ACUBV3_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000764805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein PBP2A"
                     /protein_id="HHU3848207.1"
                     /translation="MKLFDKFIDLFRVDEDNDEMTRKNEQETREETSNLDGEEVYDID
                     DITRPSKSQYQRGIRHQKENAKSRPEWLQKVDRYLPSPKNPIRRFWRRYRIGKLLFIA
                     LMAFILIFGSYLFYLSKTATVSDLQSALKTTTTIYDKNKEYAGKLSGQKGTYVELNAI
                     SDHLKNAVIATEDRTFYENNGVNFKRFFLAVATLGKFGGGSTITQQLAKNAYLSQDQT
                     IKRKAREFFLALELTKKYSKAEILTMYLNNSYFGNGVWGVEDASRKYFGTSAANLTVD
                     EAATLAGMLKGPEVYNPYYSVENATNRRDTVLAAMVDAGKLTKSQAKEAASIGMKNRL
                     ADTYAGKINDYRYPSYFDAVVNEAIDTYGISEKDIVNNGYKIYTALDQNYQSGMQKTF
                     DDTSLFPVSDYDGQSAQGASVALDPKTGGVRGLVGRVQSTKDAQFRSFNYATQSKRSP
                     ASTIKPLVVYSPAIASGWSIDKELPNKVQDFHGYKPSNYGGIETESIPMYQALANSYN
                     IPAVYTLDKLGINKAFTYGRKFGLNMSSANKELGVALGGSVTTNPLEMAQAYSTFAND
                     GIMHRAHLITRIETANGKLVKQFTDKPKRVISRSVASKMTSMMLGTFSNGTAINANVY
                     GYTMAGKTGTTETDFNPNLSGDQWVVGYTPDVVISQWVGFKNTDKHHYLTDSSAGTAS
                     NIFSTQASYILPYTKGSSFTHIENAYFQNGIGSVYNAQDASNTTNQESRSIINDLKDS
                     ASKAAQDISRAVEDSNFQEKVKDAWNSLKDYFR"
     gene            2817..3701
                     /locus_tag="ACUBV3_001722"
     CDS             2817..3701
                     /locus_tag="ACUBV3_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HHU3848208.1"
                     /translation="MSFSLKIRNPYGEHTVKELLEDYFLIPRKIRHFLRVKKHVLINN
                     EFINWQTVVQENDTITLIFDDEDYPTKKIPLGRAELIDCLYEDEHLIIVNKPEGMKTH
                     GNQPNEIALLNHVSAYSGQTCYVVHRLDMETSGAVLFAKNPFILPLINQRLERKEIWR
                     EYWALVEGKFSPKHQVLRDKIGRNRHDRRKRIIDSKNGQHAMTIIDVLKYIQNSSLIK
                     CRLETGRTHQIRVHLSHHGHPLIGDPLYNPSSNNERLMLHAHRLTLSHPLTCETISVE
                     APSSTFEKILNNYKKGVG"
     gene            complement(3726..5366)
                     /locus_tag="ACUBV3_001723"
     CDS             complement(3726..5366)
                     /locus_tag="ACUBV3_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000459235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HHU3848209.1"
                     /translation="MFDFFKKKVVKVCLVIFGIVLVSLLSLGFFYFSKGQVLSRFVAA
                     RSRTSGQAFDNIKEYMVWSDTGESITNDEANYANFEPLSKSEARKLGQEIKEGNKNDS
                     MYLKRVGSRLGIFPDYRIANKPMSLTLKTNVPKLDVLLNQKKVATSNSDHFSVTVERL
                     PRTHYTASLEGTSDGKEIKLKKDYDGKNQTIDLSVAFKSFTVTSNLMDGNLYFGDNRI
                     AKLKDGSHSVENYPVTDGSKAYIKKVFNDGEITSHKQKLISIADNQTIKLDVDGLLNE
                     KEAGQKLITAFNQLILYVSTGQDPQTLGTVFEKGAENDFYKGLKESIKAKFVTDNRKA
                     SHFTIPNIVLNKMTQVGKESYQVNFAADYDFNYDKSTDPDKKTYGHIIQNLTGNFIMK
                     KSGNSYLISNDGKKDITVAKETNKVKADPVSIFPENLVGSWKGEVEDGTVTMTFDKDG
                     KVTQKKVYKDSKSKESNHSAKVTKLEDKGNGLYLYQYESGTDTTTFVTGGIGGLKVKY
                     AYGIKIEGNKIIPVIWQTSSDGEFDYHKPLLSKPLTKQ"
     gene            complement(5536..>5910)
                     /locus_tag="ACUBV3_001724"
     CDS             complement(5536..>5910)
                     /locus_tag="ACUBV3_001724"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019318858.1"
                     /note="catalyzes the transfer of phosphate between the C1
                     and C5 carbons of pentose; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HHU3848210.1"
                     /translation="MGLSEFEKGFSFTNLVDFDALYGHRRDPHGYRDCLHEFDERLPE
                     IISAMRDKDLLLITADHGNDPTYAGTDHTREYIPLLAYSPSFTGNGLIPVGHFADISA
                     TVADNFGVDTAMIGESFLQDLV"
BASE COUNT         1837 a         1159 c          933 g         1981 t
ORIGIN      
        1 atctaagggt ttgggttgtt tctatctaaa ataatctatt attaaaatcg attaatcaag
       61 tccatgactc ctgcggctaa cagtttgtca aagatgtaga taacgattgt gaaaaataca
      121 gtgtattcca agatggaaat aaaatctttc caacgttgtt ttcgatttgg ccaagtagtg
      181 tcacgcaata cttgaaaaat ccctttgaca aacattatca gttctccttt ttgcagtttg
      241 ttacgctata tcaacgcgtt tctttgtgta ttgtatactt cttacaatat ttacaaaatt
      301 tatttacttc tagtcgtgtt ggcttggggg tgctacttac tccgattgag taatttcggc
      361 ttccgcattc tgtacacgct aggcttgctt tcttttgtgc cataacgcct ccataagtgc
      421 tattttaaca tgatttgtat aatcctgcaa gctatctaaa gtagtccttt agactattcc
      481 aagcatcttt taccttctct tggaaatttg aatcttccac cgctctagaa atatcttgtg
      541 cagccttact tgccgaatct tttaaatcat taataattga gcgtgattct tggttcgttg
      601 tattactagc atcttgtgca ttataaactg aaccaatacc gttttggaaa taagcattct
      661 caatatgagt aaaactagac cccttagtat acggtagtat atatgaagct tgcgtgctaa
      721 atatatttga agctgtacct gcactagaat ctgtcaaata atggtgttta tctgtgttct
      781 taaagccaac ccattgacta ataacgacat caggcgtata gccgactacc cactggtctc
      841 cagataaatt ggggttaaaa tctgtttcgg ttgttccagt tttacctgcc attgtataac
      901 cataaacatt ggcattaata gctgtaccat ttgagaatgt tcctaacatc atactagtca
      961 ttttactagc cactgagcga ctaataactc gcttaggctt atctgtaaat tgctttacta
     1021 attttccatt agctgtctca attcgagtaa tcaaatgagc gcgatgcatt ataccgtcat
     1081 ttgcaaaagt tgagtaagct tgtgccattt ctaaaggatt agtggttaca cttccaccta
     1141 gcgccactcc tagctctttg tttgctgatg acatatttag accaaacttc ctgccataag
     1201 taaaggcttt attgattcct agtttgtcta aagtataaac agcaggaata ttataagaat
     1261 tagctagtgc ttgatacatc gggatacttt cagtctcaat cccaccatag ttacttggtt
     1321 tataaccatg aaaatcttga actttattag gaagttcttt atctattgac catcctgaag
     1381 caatagctgg tgaataaaca actaaaggtt ttatggttga ggctggactt ctctttgatt
     1441 gagtcgcgta gttaaaactt ctaaactgtg catctttagt actttgaaca cgtccaacta
     1501 aacctcttac accaccagtt tttggatcta aagctacgct agcaccttgg gcagattgac
     1561 catcatagtc agatacaggg aagaggctag tatcatcaaa tgttttttgc attcctgatt
     1621 gataattttg atctaatgca gtgtaaatct tatagccatt attgacaata tctttttctg
     1681 aaataccata agtatcaatg gcttcattga caacagcatc aaaataggat gggtacctgt
     1741 aatcattaat tttaccagca taggtatctg ctaatctatt tttcatacca attgaagcag
     1801 cttctttagc ttgagacttg gttagtttac ctgcatcaac catcgctgcc aaaacagtat
     1861 ctcttcggtt agttgcattt tcaacagaat aataaggatt ataaacttct ggtcctttaa
     1921 gcattccagc aagtgtagca gcctcatcta ctgtcaaatt agcagcactg gttccaaaat
     1981 atttcctact agcatcttcc acaccccaaa caccattacc aaaataagag ttattaagat
     2041 acatggtcag aatctcagcc ttgctatatt tcttagtcaa ttctagcgct aaaaagaatt
     2101 cacgcgcctt gcgtttaatc gtttggtcct gtgacaagta agcatttttt gctaattgct
     2161 gggtaatagt tgagcctccg ccaaatttcc caagtgtagc tactgccaaa aaaaatcgct
     2221 taaagtttac accattattt tcataaaaag ttctatcttc agttgctata acagcatttt
     2281 tgagatgatc agaaatagca tttaactcta cataagttcc cttttgtccc gaaagttttc
     2341 cagcatattc tttattctta tcataaatgg tggtggttgt ttttagagca ctctgtaaat
     2401 cactaactgt agcagtttta gaaagataaa aaagatagct accaaatata agaataaaag
     2461 ccattaaggc gataaaaagc aacttcccaa tacgatagcg ccgccaaaaa cgacggatag
     2521 gatttttagg agaagggagg taacgatcaa ctttttgtag ccattcaggt cgagatttag
     2581 cattttcctt ttggtgacga atgcctcgct gatactggga tttactaggt cgggtaatat
     2641 catcaatatc atagacttct tcgccatcca aattgctagt ttcttctcta gtttcttgtt
     2701 cattttttct tgtcatttcg tcgttgtctt catcaacacg aaataagtct ataaacttat
     2761 caaataattt catagactta ttatatcaat tttcaataaa atgctataat aaaaccatgt
     2821 cattttcatt aaaaattaga aatccatacg gtgaacatac cgttaaagaa ctccttgaag
     2881 attatttttt gattccacgt aagattagac attttttgcg tgttaaaaaa catgtactta
     2941 taaacaatga atttattaat tggcaaactg tcgtccaaga aaacgatact attaccttaa
     3001 tctttgatga tgaggattac cctactaaaa aaattcctct gggcagagca gagcttattg
     3061 attgtcttta tgaggatgaa catcttatta tcgttaataa acctgaaggt atgaaaactc
     3121 acggtaacca accaaatgaa atagcactgt taaatcatgt atctgcctat tctggacaaa
     3181 catgctatgt tgttcatcgc ctagatatgg agaccagtgg agctgtttta tttgctaaaa
     3241 atccatttat acttcccctt atcaatcaac gcttagaacg aaaagaaatt tggcgtgaat
     3301 attgggcttt agttgaagga aaattttcac ctaagcatca agttttgaga gacaaaattg
     3361 gacggaaccg tcatgacaga cgtaaacgaa tcattgattc taaaaacggt caacatgcta
     3421 tgacaatcat tgacgttttg aagtatatcc aaaatagtag tctcataaaa tgccgactgg
     3481 aaaccggaag aacccatcaa attcgcgttc acttatctca tcatggacat cccttaatag
     3541 gagaccccct ctacaaccct tcttctaata atgaaaggtt aatgctacac gctcaccgat
     3601 tgactctatc tcatccatta acttgcgaaa ctattagcgt agaggcccct tcatctactt
     3661 tcgagaagat tttaaacaat tataaaaaag gagttggata ataattatcc aactcctttt
     3721 aatcattatt gcttagttaa tggcttactg agtagtggtt tatggtaatc aaattctcca
     3781 tcactagatg tctgccagat cactgggatg atcttattcc cttcaatttt tattccatag
     3841 gcgtatttaa ctttaagacc accgatacct cctgttacaa aagttgtagt atcagtacct
     3901 gattcatatt gataaaggta gagcccattt cccttatcct ccaacttcgt aactttagca
     3961 gaatggttac tttccttaga tttactatcc ttgtaaactt ttttctgagt aactttacca
     4021 tccttatcaa aagtcatcgt tactgtaccg tcctcaactt cacctttcca acttcctaca
     4081 aggttctctg gaaaaatgga aacgggatca gccttaactt tattagtttc cttagctact
     4141 gtgatatctt ttttaccatc attagaaata aggtaactat ttcccgattt tttcataata
     4201 aaattaccag ttaaattctg aataatgtgg ccatatgttt tcttatcagg atctgttgat
     4261 ttatcataat taaaatcata atcggcagca aaattaactt gataactttc tttcccaacc
     4321 tgtgtcattt tattaaggac aatattagga atagtgaaat ggctcgcttt acgattatca
     4381 gttacaaatt ttgctttaat actttctttc aatcctttgt aaaaatcatt ttcggcacct
     4441 ttttcaaaaa ctgttcctaa agtctgaggg tcttgcccag ttgatacata aagtatcaat
     4501 tgattgaaag cagttatcaa tttctgtcca gcttcttttt catttaataa accatcaaca
     4561 tctaatttga ttgtctgatt atcagcgata gagattaatt tttgtttatg agaggttatc
     4621 tcaccatcat taaaaacttt tttgatataa gcttttgaac catcagtcac tggataattc
     4681 tctacggaat ggctaccatc ttttaattta gcaatacgat tatcaccaaa ataaagatta
     4741 ccatccataa gattacttgt tactgtaaaa gatttaaaag cgaccgataa atcaatagtt
     4801 tggtttttac catcataatc ttttttgagt ttaatctctt ttccgtcact agtcccttca
     4861 aggctagcag tataatgggt tctaggtaaa cgttccaccg ttactgaaaa atgatctgaa
     4921 ttgcttgtcg cgaccttttt ttgattcaac aaaacatcca atttaggaac attggttttc
     4981 aaggtcaaag acataggctt attagcaata cgataatcag ggaatatacc taagcgacta
     5041 cctacacgct ttagatacat tgaatcattt ttgtttcctt cttttatttc ctgccctaat
     5101 ttacgcgcct cacttttaga caagggctca aaattagcgt aattggcctc atcatttgtg
     5161 atagattctc ccgtatctga ccaaaccatg tattctttaa tattatcaaa cgcttgacct
     5221 gacgtacgag aacgtgctgc tacaaaacga ctaagtactt gtccctttga aaaataaaag
     5281 aatcctagac tcagaagtga tactaaaact ataccaaaaa taactaagca cactttaaca
     5341 acttttttct taaaaaaatc aaacatacct tatcctctca ttcactttat attaaagtaa
     5401 attgcttaaa gaaaagttac aacactgata aaacagctgt atttgtactt gtaaccgcta
     5461 tttttctatt ataacaaaac gaaaaaagga ttgaaaccat aatccgattt caacctctaa
     5521 aatttttgtc tatgattata ctaagtcctg caagaagctt tctccaatca tcgcagtatc
     5581 aacaccaaag ttatccgcaa ctgttgctga aatatctgcg aaatgcccta ctgggataag
     5641 tccgtttcct gtgaaggatg ggctataggc taagagagga atgtattctc ttgtatggtc
     5701 tgtgcctgca taagttgggt cattaccatg atcggcagtg atcaaaagta agtctttgtc
     5761 acgcattgct gaaatgatct ctggaagacg ctcatcaaac tcatgaagac aatcacgata
     5821 accatgagga tcacgacggt gaccatagag agcatcaaag tcaaccaaat tagtaaagga
     5881 gaagcctttt tcaaactctg ataaccccat
//
LOCUS       DBJYSH010000110         5862 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.110, whole genome shotgun sequence.
ACCESSION   DBJYSH010000110 DBJYSH010000000
VERSION     DBJYSH010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 5862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5862
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.110"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            191..2059
                     /gene="glgB"
                     /locus_tag="ACUBV3_001725"
     CDS             191..2059
                     /gene="glgB"
                     /locus_tag="ACUBV3_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HHU3848211.1"
                     /translation="MSNKDELYTFGIGENFHLQNYLGVHSENGSFCFRVWAPNAENVQ
                     VIGDFTDWRNRPLQMNKIQAGVWEANSLDAREGDLYKYLVTRKGGQVVEKIDPMAVYM
                     ERRPGTASVIKVLRNKKWEDGLWMGRRKRLGFQKRPINIYEVHAGSWKKDDFGHPMTF
                     SQLKDYLIPYLVEMNYTHVEFMPLMAHPLDMSWGYQLMGYFAFEHTYGTPEEFQDFVE
                     ACHKNNIGVLVDWVPGHFIQNDDALAYFDGTATYEYQNHDRAHNYRWGALNFDLGKNQ
                     VQSFLISSALFWIEHYHIDGIRVDAVSNMLYLDYDEGPWEANQFGDNRNLEGYHFLRK
                     LNKVIKERHPNVMMIAEESTASTPITKDLESGGLGFDFKWNMGWMNDILRFYEEDPLY
                     RQYDFNLVTFSFMYIFNENFVLAFSHDEVVHGKKSMMHKMWGDRYNQFAGLRNLYAYQ
                     MCHPGKKLLFMGSEFGQFLEWKYNDQLEWENLNDDMNQKMQRYTKQLNQFYKDHKCLW
                     RIDDSFDGLEIIDADNKSETVLSFIRKDDKDDLLLCVFNMTPVERPNFTIGVPQAGIY
                     EEVLNTEMEEFGGVWKNHNPVTKTQVATWKDYDHTLSFTLPALGASVWRIKRRLRK"
     gene            2101..3240
                     /locus_tag="ACUBV3_001726"
     CDS             2101..3240
                     /locus_tag="ACUBV3_001726"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019785022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HHU3848212.1"
                     /translation="MKNEMLALIFVGGQGTRLGKLTQSIAKPAVQFGGRYRIIDFALS
                     NCANSGINNVGVITQYQPLELNTHIGNGSSWGLDGIDSGVTVLQPYSATEGNRWFQGT
                     SHAIYQNIDYIDRINPEYVLILSGDHIYKMDYDDMLQTHKDNLASLTVAVLDVPLKEA
                     SRFGIMNTDSNDRIVEFEEKPEHPKSTKASMGIYIFDWKRLRTVLIDGEKNGIDMSDF
                     GKNVIPAYLESGERVYTYNFDGYWKDVGTIESLWEANMEYIGEDNKLHSRDRSWKIYS
                     KNLIAPPNFMTEDANVKDSLVVDGCFVAGNVEHSILSTNVQVKPNAIIKDSFVMSGAT
                     IGEGAKINRAIIGEDAVIGDGVVIDGSKEVEVIGYKEVAGVPNED"
     gene            3230..4363
                     /gene="glgD"
                     /locus_tag="ACUBV3_001727"
                     /pseudo
     CDS             3230..4363
                     /gene="glgD"
                     /locus_tag="ACUBV3_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000687083.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
     gene            4360..5790
                     /gene="glgA"
                     /locus_tag="ACUBV3_001728"
     CDS             4360..5790
                     /gene="glgA"
                     /locus_tag="ACUBV3_001728"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - alpha-1,4-glucan
                     glucosyltransferase (UDP-glucose donor) activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HHU3848213.1"
                     /translation="MKIMFVAAEGAPFAKTGGLGDVIGALPKSLSKKGHDVAVVMPYY
                     DMVDQKFGDQIENLMYFYTDVGWRHQYVGVKRLSQDNVTFYFIDNQYYFYRGHVYGDW
                     DDGERFAYFQLAALELMEKIDFIPDVLHVHDYHTAMIPFLLKEKYHWIQAYNNIRAVF
                     TIHNIEFQGQFGPEMLGDLFGVGAERYEDGTLRWNNCLNWMKAAILYSDRVTTVSPSY
                     ANEIKTPEFGKGLDQIMRMEAGKLSGIVNGIDSDLLNPETDAFLPYHFSKSNLGGKIK
                     NKLALQENLGLPQDKNVPLIGIVSRLTDQKGFDIIASELDNMLQQDIQMVILGTGYHH
                     FEETFSYFASRYPEKLSANITFDLGLAQQIYAASDIFMMPSAFEPCGLSQMMAMRYGS
                     LPLVHEVGGLKDTVVAFNQFDGSGTGFSFNHFSGYWLMQTLKLALEVYNDYPEAWKKL
                     QWQAMSKDFSWDTACVAYEQLYQQLQ"
BASE COUNT         1866 a          845 c         1223 g         1928 t
ORIGIN      
        1 ggttattttt gtgtatatta aggaatttga tttgaatttg agtttcttat attttagtta
       61 atttttcgga aatatagagg gtgaaatatt ttcagatgcg tctgtatgag gaaaatgcat
      121 tcctcttttt ttgtaccatt gaggaaatat ttttgtctta tattgactag tagacctgtg
      181 aggtgagtag atgtccaata aagatgagtt atatactttt ggaataggtg aaaattttca
      241 tctccagaat tatttaggcg tacatagcga aaatggaagc ttttgtttca gagtttgggc
      301 tcctaatgct gaaaatgttc aagttattgg agattttaca gattggcgaa atcgacctct
      361 ccaaatgaac aaaattcaag caggagtttg ggaagctaac agtttggatg cgcgcgaagg
      421 tgatttatat aaatatttgg ttactcgtaa aggtggtcaa gtagttgaga aaatcgatcc
      481 tatggcagtt tatatggaaa gacgtcctgg aactgcaagt gttattaaag ttcttagaaa
      541 taaaaagtgg gaagatggtc tttggatggg aaggcgcaaa cgtctgggtt ttcagaagcg
      601 tccaattaat atttacgaag ttcatgctgg ttcatggaaa aaagatgatt ttggacatcc
      661 tatgactttt tctcagttaa aagattactt aatcccctac cttgttgaga tgaattatac
      721 tcatgttgaa tttatgccct taatggcaca tccgcttgat atgagttggg gatatcaatt
      781 aatggggtat tttgcttttg aacataccta tggtactcca gaagaatttc aagactttgt
      841 agaagcatgt cataaaaata atataggagt tcttgtagat tgggttccag gacactttat
      901 tcagaatgac gatgctttag cctatttcga tggaactgct acttatgagt atcaaaatca
      961 tgatcgtgcg cataactatc gatggggagc tctgaacttt gatttaggaa aaaatcaagt
     1021 acaatccttt ttgatttcga gtgccttgtt ctggattgaa cattatcata ttgatggtat
     1081 tcgtgttgat gcggttagta atatgttata tctcgattat gatgaaggac catgggaagc
     1141 taaccaattt ggggataatc gtaatttgga aggatatcac tttttaagga aacttaacaa
     1201 agtaattaaa gagcgtcatc ctaatgttat gatgattgct gaagagtcaa cagcttctac
     1261 cccaataact aaagacttag agtctggagg attgggtttt gactttaaat ggaatatggg
     1321 ctggatgaat gatatcctgc gtttttatga agaagatcct ctctataggc aatatgattt
     1381 taatttagtg acttttagtt ttatgtatat attcaatgag aactttgtac tagctttttc
     1441 acatgatgaa gtagtacatg gtaaaaagag tatgatgcat aaaatgtggg gtgaccgtta
     1501 taatcaattt gctggtttac gtaatttata tgcatatcaa atgtgtcatc ctgggaaaaa
     1561 acttctcttt atgggaagtg agtttggtca atttttagaa tggaaatata atgatcaact
     1621 agaatgggaa aacttaaatg atgatatgaa ccaaaagatg caacgctata ctaaacaatt
     1681 gaatcaattt tataaagatc ataagtgcct atggcgtatt gatgattcct ttgacgggct
     1741 agaaattatt gatgcggata ataaatctga aactgtttta tcttttatac gaaaagatga
     1801 taaagacgat ttgttacttt gtgtctttaa tatgactcct gtagaacgcc ctaactttac
     1861 tattggtgtt ccgcaagcgg gtatttatga agaagtttta aatactgaaa tggaagagtt
     1921 tggaggtgtt tggaaaaacc acaatcctgt aacgaaaaca caagtggcta catggaaaga
     1981 ttatgatcat actttaagtt ttacattacc agcccttggg gcaagtgttt ggagaataaa
     2041 acgtcggctt cgtaaataag tctgcaattt agtaacctca ttttgaaagg aaagaataat
     2101 atgaaaaatg aaatgttagc actcattttc gtcggaggac aaggcactcg actcggaaaa
     2161 ttgactcaga gcattgcgaa acctgctgtt caatttggag gtcgttaccg tattattgac
     2221 tttgcgcttt caaattgtgc gaattcaggt attaataatg ttggagttat tactcagtat
     2281 caaccgctgg aactcaatac tcatattgga aacggatcta gctggggact agatggtatc
     2341 gattcgggag taactgtatt acagccatat tctgcaacag aaggtaatcg ctggtttcaa
     2401 ggtaccagcc acgctatcta tcaaaatatt gactacattg atcgtattaa tccagaatat
     2461 gtactcattc tctctgggga ccacatctat aaaatggact atgatgacat gctacagacg
     2521 cataaagata atttagcaag cctaacagtc gctgttttag atgttccttt aaaagaagca
     2581 agccgttttg gaattatgaa tactgattca aacgaccgta ttgtcgaatt tgaagaaaaa
     2641 cctgaacatc ctaagtcaac aaaagcttca atgggaatat acatttttga ctggaagaga
     2701 ttacggacag ttttaattga tggtgaaaag aacggcatag acatgtcaga ctttggtaaa
     2761 aatgttatac cagcatacct tgaatcgggt gaacgtgttt atacctataa ttttgatggg
     2821 tattggaaag atgttggtac tattgaatcc ctttgggaag ctaatatgga atatattgga
     2881 gaagataaca aattacatag tcgtgatcgt tcatggaaaa tctattctaa aaatttgatt
     2941 gcaccaccta atttcatgac agaagatgca aatgttaaag attctttagt tgtagatgga
     3001 tgctttgttg caggaaatgt tgagcactcc attttatcaa ctaatgtcca ggtaaaacca
     3061 aatgctatta ttaaagactc ctttgttatg agtggcgcga ctatcggtga aggtgcaaag
     3121 ataaatcgag ctattatcgg tgaggatgct gttattggtg atggtgttgt gattgatgga
     3181 agtaaagaag tagaagttat tggttataaa gaagtagcgg gggttccaaa tgaagattga
     3241 taaatatact gcgattttag gaaatactgt tagttaccca gatatgcaca gtttgacgga
     3301 taatcgtccg cttgcttctc taccttttga tggtaagtat cgtttaattg attttcaatt
     3361 atcctcttta gctaatgcag gcgttagaag tatttatggt atttttcgtg ggaataatgt
     3421 tcgctctgtt tttgaccata ttagaagtgg tcgtgagtgg ggcttaggta cactgttgag
     3481 tcactattct ttaggattct atgatacaga cgatgatact cgtacagtca atggggatta
     3541 ttacgattaa attttaactt acttgaggcg ttcgggtagc gaccagacga tatatatgag
     3601 ttgtgatatg ctttgtaata ttgacttaag tcaagtaatc cacttgcata atgcaaatgg
     3661 tgtgcaagcc accgtggttt ataaaaaaat gccgagatca gctattgcag aggctaatca
     3721 agtcttgacc attgatgaat ctgatcatgt agtgagtcta ggtaaagctg tagataatga
     3781 cgataaccaa aaaatgtctg ctgagattta tgttatcgat acaccttggt taatcgaaaa
     3841 aatggaagag gaagctcaaa ataatgaacc acgtaaactc cgcttcttac ttcgtgattt
     3901 aattgttgaa agtaatgctt tggcttttga atacactggt tatttgtcaa acatttcttc
     3961 tattaagtcc tattatgatg ctaacatgga catgttaaca ccaaataaat tttattcgct
     4021 cttcttttca aaccaaaaag tttatactaa ggtgaaaaat gaggaagcta cttattttga
     4081 taaacaatca aatgtctcta attcgcaact agcctatgga agtattatta aaggttattt
     4141 agaccattct atcgtttcta gaaattgtct ccttgaaaaa gggactcgag tagtcaatag
     4201 tattatcttt ccaaaagtaa aaatcggaga aggggcaacc attgaaaata ctattattga
     4261 caaatgcgtt aaggttgcat ccggagttac attgaaaggt agtttagata aaccgttagt
     4321 gattcctaaa ttttcagaga ttaatgagga tattatacaa tgaaaataat gtttgttgcg
     4381 gcagagggtg ctccctttgc taaaacaggt ggtttgggag atgtgattgg cgcactccca
     4441 aaatcactta gtaaaaaagg tcatgatgtc gctgttgtca tgccatatta tgatatggta
     4501 gaccaaaaat ttggagacca aatagaaaac ctcatgtatt tctatacaga tgttggctgg
     4561 cgtcatcaat atgtaggagt taagagactt tctcaagata atgttacgtt ctactttatt
     4621 gataatcaat attattttta tagaggtcat gtctatggtg attgggatga tggggagcgt
     4681 tttgcttatt tccaattagc tgctttagag ttgatggaga aaatagattt tattcccgat
     4741 gtcttacacg tccatgatta tcatacagca atgattcctt tcttattgaa agagaagtac
     4801 cactggattc aggcctataa taatataaga gcagttttta ctattcacaa tattgaattt
     4861 caagggcagt ttggtccaga aatgttagga gatttatttg gagttggcgc tgagagatat
     4921 gaagatggaa ctcttagatg gaataattgt cttaattgga tgaaagcagc aattctttac
     4981 tcagatagag tgacaacggt atcaccaagc tacgcaaatg aaataaaaac tccagaattt
     5041 ggtaaaggtt tagaccaaat tatgcgtatg gaagcaggta aattatctgg aatcgtaaat
     5101 ggtattgaca gcgatttgtt aaatccagaa acagatgctt ttttgccata tcatttttca
     5161 aaaagcaact tagggggcaa aattaaaaat aagttggctt tacaggaaaa tttgggcttg
     5221 cctcaagata aaaatgtacc attaattggt attgtttcac gacttaccga tcaaaaagga
     5281 tttgatatta ttgcttctga attggacaat atgcttcaac aagatattca aatggttatt
     5341 ttaggaacag gctatcacca ttttgaagaa acgttctctt attttgctag tcgttaccct
     5401 gaaaaactat ctgctaatat tacttttgac ttaggattag cgcaacagat ttatgcggct
     5461 tcagatatct tcatgatgcc aagtgctttt gaaccttgtg gtctctcgca gatgatggct
     5521 atgagatatg gtagcttacc cttggtacat gaagttggag gattaaaaga tactgtggta
     5581 gccttcaatc aatttgatgg tagtggtaca ggtttttcat ttaatcattt ttcaggctat
     5641 tggttaatgc aaactctaaa actagcatta gaagtttata atgattatcc tgaagcgtgg
     5701 aaaaagttac aatggcaagc tatgtcaaaa gatttttcat gggatacagc atgtgttgct
     5761 tacgaacaac tgtatcagca actacaataa tcactttatt ttagtgatta gttgataagc
     5821 ttatcgaatc tctatgctaa acgtttctta tgtaaaacca ct
//
LOCUS       DBJYSH010000111         5848 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.111, whole genome shotgun sequence.
ACCESSION   DBJYSH010000111 DBJYSH010000000
VERSION     DBJYSH010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 5848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5848
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.111"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            117..1772
                     /locus_tag="ACUBV3_001729"
     CDS             117..1772
                     /locus_tag="ACUBV3_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HHU3848214.1"
                     /translation="MAKQKNNWRRVGVGVLTLASVATLAACGSKSASQDSNGAINWAI
                     PTEINTLDLSKVTDTYSNLAIGNSSSNFLRLDKDGKTRPDLATKVDVSKDGLTYTATL
                     RKGLKWSDGSKLTAKDFVYSWQRLVDPKTASQYAYLAVEGHVLNADKINEGQEKDLNK
                     LGVKAEGDDKVVITLSSPSPQFIYYLAFTNFMPQKQEVVEKYGKDYATTSKNTVYSGP
                     YTVEGWNGSNGTFTLKKNKNYWDAKNVKTKEVRIQTVKKPDTAVQMYKRGELDAANIS
                     NTSAIYQANKNNKDVTDVLEATTAYMQYNTTGSVKGLDNVKIRRALNLATNRKGVVQA
                     AVDTGSKPAIAFAPTGLAKTPDGTDLAKYVAPGYEYNKTEAAKLFKEGLAESGLTKLK
                     LTITADADVPAAKNSVDYIKSTWEAALPGLTVEEKFVTFKQRLEDSRKQNFDIVVSVW
                     GGDYPEGSTFYGLFKSDSQNNDGKFANKDYDAAYNKAISEDALKPEESAKDYKEAEKI
                     LFEQGAYNPLYFRSGKGLQNPKLKGVIRNTTGLSIDFTHAYKK"
     gene            1891..2805
                     /locus_tag="ACUBV3_001730"
     CDS             1891..2805
                     /locus_tag="ACUBV3_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HHU3848215.1"
                     /translation="MIKYILKRVAILLVTLWVVITLSFFLMQILPGTPYNNPKLTEEM
                     IALLNKQYGLDKPVWQQYLTYLWNVLHGDFGTSYQSVNQPVSRMISLRLGVSVHLGVQ
                     ALVFGVLGGILVGAISARHKNDKVDGILSVIATLGISMPSFIIGILLLDYFGFKWNLL
                     PLSGWGTFSQTILPSLALGLPTLASVSRFFRSEMIETLNSDYVQLARSKGMTIRQVTR
                     KHAYRNSMIPILTLIGPLAAGLLTGSALIEQIFSIPGIGQQFVTSIPTKDYPVIMGTT
                     IVYAVMLMVAILITDVVISIVDPRVRLQ"
     gene            2815..3846
                     /locus_tag="ACUBV3_001731"
     CDS             2815..3846
                     /locus_tag="ACUBV3_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000764054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HHU3848216.1"
                     /translation="MADKNRTFKLVGAGSSSTQEKIEKPALSFMQDAWRRLKKNKLAV
                     VSLYLLALLLTFSLASNLFVTQKDANGFDSKKVTTYRNLPPKLSSNLPFWNGSIKYAG
                     NTESTDAYKSQNVPEKVKYALGTDSLGRSVAKRIIVGIRISLLVAIAATFIDLIIGVT
                     YGLVSGFAGGRLDTLMQRIVEVISSIPNLVIVTMLGLVLGNGITAIIISIAFTGWTSM
                     SRQVRNLTLSYREREFVLAARSLGESPIKIAFKHILPNISGIIIVQIMMTIPSAIMYE
                     AVLSAINLGVKPPTASLGSLISDAQENLQYYPYQVILPALALVMISLAFILLGDGLRD
                     AFDPKSSDE"
     gene            3859..4905
                     /locus_tag="ACUBV3_001732"
     CDS             3859..4905
                     /locus_tag="ACUBV3_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HHU3848217.1"
                     /translation="MEKETILSVNNLHVDFHTYAGEVKAIRDVNFELKKGETLAIVGE
                     SGSGKSVTTRTLIGLNAKNSEISGNVQFKGRNLVELSEEEWTKVRGNEISMIFQDPMT
                     SLDPTMKIGMQIAEPMMIHQKISKKDALKLALELMKDVGIPNAEEHINDYPHQWSGGM
                     RQRAVIAIALAADPEILIADEPTTALDVTIQAQILNLMKKIQAERDSSIVFITHDLGV
                     VAGMADRVAVMYAGKIVEFGTVDEVFYNPQHPYTWGLLNSMPTTDTESGSLESIPGTP
                     PDLLNPPKGDAFAARNEFALDIDHEEEPPYFKVSETHFAATWLLDERSPKVLPPLPIQ
                     KRWEKWNEIEGRKA"
     gene            4905..5837
                     /locus_tag="ACUBV3_001733"
     CDS             4905..5837
                     /locus_tag="ACUBV3_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HHU3848218.1"
                     /translation="MTENRKKLVEVKNVSLTFNKGKANEVRAIDNVSFDIYEGEVFGL
                     VGESGSGKTTVGRSILKLYDISDGEITFNGEVISHLKGKALHSFRKDAQMIFQDPQAS
                     LNGRMKIRDIVAEGLDIHKLAKSKSDRDSKVQALLDLVGLNKDHLTRYPHEFSGGQRQ
                     RIGIARALAVEPKFIIADEPISALDVSIQAQVVNLMQKLQREQGLTYLFIAHDLSMVK
                     YISDRIGVMHWGKLLEVGTSDDVYNNPIHPYTKSLLSAIPEPDPESERQRVHQPYNPA
                     IEQDGQERQMHEITPCHFVLSTPQEAEEYKKQIL"
BASE COUNT         1900 a          988 c         1163 g         1797 t
ORIGIN      
        1 atgtaaaaaa atgaaagtta aaaaattatc tgaaaatttt gcgtttttgt cttttcttaa
       61 gaattttttg tggtataatg ttagtcacta gataaatgaa ttaggagaac aaagctatgg
      121 caaaacagaa aaataactgg cgccgtgttg gagttggtgt ccttacactt gcttcagttg
      181 cgactcttgc tgcatgtgga agtaaatcag cttcccagga ttctaatgga gcgattaatt
      241 gggctattcc aacagaaatc aatacactag atttatctaa agttacagac acttactcaa
      301 atctagctat tggtaactct agtagtaatt tccttcgctt agataaggat ggaaagacga
      361 gaccagattt ggctactaaa gttgatgttt caaaagatgg tttaacttat acagctacat
      421 tacgtaaagg cttgaagtgg tcagatggca gtaaacttac tgcaaaggat tttgtttatt
      481 catggcaacg tttagttgat cctaagacag cttcacaata tgcttacctt gctgttgaag
      541 ggcatgtgct taatgccgat aaaatcaacg aaggacaaga gaaagacttg aataagctag
      601 gtgttaaggc agaaggcgat gacaaagttg ttattacttt atctagtccg tctccacaat
      661 tcatctacta ccttgcattc actaatttca tgccacaaaa acaagaagtt gttgaaaaat
      721 atggaaaaga ttacgcaact acttcaaaaa atacagttta ctcaggacca tatactgttg
      781 aaggttggaa tggttcaaat ggtactttca ccctgaagaa aaacaaaaat tattgggacg
      841 ctaaaaatgt aaaaacaaaa gaagttcgca tccagactgt taaaaaacca gataccgccg
      901 ttcaaatgta taaacgtggt gaattagatg cagctaatat ctcaaatact tctgctattt
      961 atcaagctaa taaaaataat aaagatgtca cagatgttct agaagcgacc actgcctata
     1021 tgcaatataa tactactggt tctgtgaaag ggcttgataa tgttaagatt cgtcgcgcct
     1081 taaacttagc aactaaccgt aaaggagttg ttcaagcagc cgttgataca ggctcaaaac
     1141 cggcaattgc ttttgcaccg actggtttag ccaaaacacc agatggaact gatttggcaa
     1201 aatatgttgc cccaggttat gaatataata aaactgaagc agcaaaactc tttaaagaag
     1261 gtttggctga atcaggcttg actaagctaa aattaacaat tacagcagat gctgatgttc
     1321 ctgctgccaa aaactctgtt gactatatca agtctacttg ggaagctgct cttccaggac
     1381 ttactgttga agaaaaattt gtaaccttta aacaacgctt agaagacagt agaaaacaaa
     1441 actttgatat cgtagtttct gtttggggtg gggattatcc agaagggtca actttctacg
     1501 gcctctttaa gtcagattca caaaataacg atggaaaatt tgctaacaag gactatgacg
     1561 ctgcttataa caaggcaatt tccgaagatg ccttaaaacc agaagaatca gcaaaggact
     1621 ataaagaagc agagaaaatt ctatttgagc aaggcgctta taacccactc tacttccgta
     1681 gtggtaaagg tttacaaaat ccaaaattaa aaggtgttat tcgtaatact acaggtttgt
     1741 caatagactt tacacatgct tataaaaaat aattaaatta ctttacacta aacctttgca
     1801 agaattggta attgccagtt cttgcttttg gttaagtaaa gaaagtgaaa gagtttgcta
     1861 tttttagcaa ttctaagaaa aggaaatgat atgattaaat atattttaaa gcgtgttgct
     1921 attttgttag taacgctatg ggttgtcata accctatcgt tctttctgat gcagatcctt
     1981 ccaggaacac cctacaataa tccaaaactc actgaagaaa tgatcgcatt acttaataaa
     2041 cagtatggtt tagacaagcc tgtatggcaa caatatttga cttacctttg gaatgtactt
     2101 catggtgact ttggaacgag ttatcaatca gtgaatcagc cagtatcacg tatgattagc
     2161 ttgagattag gagtttcagt acatctaggt gttcaggctc tcgtatttgg tgttttaggc
     2221 ggtattttgg taggagctat ttcggcacgt cataagaatg ataaagtcga tggtatctta
     2281 agtgttattg caacgctagg gatttctatg ccttcattta tcattggtat tttattatta
     2341 gactattttg gatttaagtg gaacttgttg cctttatcag gttgggggac tttctctcaa
     2401 acaattttac cttctttagc tctaggactc cctacattag catctgtttc acgcttcttc
     2461 cgtagtgaaa tgattgaaac tttaaattct gattatgtgc aactagctcg ttcaaaaggt
     2521 atgacaattc gtcaagtaac tcgaaaacat gcttaccgta attctatgat tccaatttta
     2581 actctaatcg gccctttggc ggcgggtctg ttaactggtt ctgcactaat tgaacagatc
     2641 ttttcgattc caggaattgg gcagcagttt gttacttcga ttccaacaaa agactatcct
     2701 gttattatgg ggacaaccat tgtttatgca gtaatgttga tggttgctat cttgataaca
     2761 gacgttgtta ttagtatcgt agacccacgc gttcgcttgc agtaaggaga tgttatggca
     2821 gataaaaaca gaacatttaa acttgtaggt gcaggatctt ctagcacaca agaaaaaatt
     2881 gaaaagcctg ctctttcgtt tatgcaagat gcgtggcgtc gcttgaaaaa aaacaagtta
     2941 gcagtggttt cactctattt attagctctt ttacttactt tttcgttagc ctcaaattta
     3001 tttgtaactc agaaggatgc taatgggttt gattcaaaaa aagtaacgac atatcgcaac
     3061 ttaccaccta aattgagttc aaaccttcct ttttggaatg gtagcattaa gtacgctgga
     3121 aatacagaat ctacagacgc ttataagtct caaaatgtcc ctgaaaaagt taagtatgct
     3181 ttaggtacag attctctagg gagaagtgtt gctaagcgca tcatcgtagg tattcgaatt
     3241 tccttacttg tagctattgc agctacattc attgatttaa ttatcggtgt tacatacggt
     3301 cttgtttcag gttttgctgg aggacgcctt gatacattaa tgcaacgtat cgtagaggta
     3361 atttcatcaa ttccaaactt agttatcgtt acaatgttag gtttggtcct tggtaacggt
     3421 attacggcta ttattatctc tattgctttt accggatgga cttctatgtc tcgtcaggta
     3481 aggaatttaa ccctatcata tcgagaaaga gagtttgtgc ttgctgcacg ttcacttggg
     3541 gaaagtccga ttaaaatagc tttcaaacat attttaccta acatttctgg tattatcatt
     3601 gtacaaatta tgatgacaat cccaagtgct attatgtatg aagcagtact ttcagcgatc
     3661 aatttaggtg ttaaaccacc gacagcttca ttaggatcat tgatttcaga tgcacaagaa
     3721 aatttacaat attatccata tcaagttatc ttgccagctt tagcattggt aatgatttca
     3781 ttggcattta ttttgcttgg tgatgggttg cgagatgctt ttgatccaaa atctagcgat
     3841 gaataaggag gtataatcat ggaaaaagaa actattttaa gtgttaataa tcttcatgtt
     3901 gacttccaca catatgctgg agaagtaaaa gcaattcgtg atgtcaactt tgaattaaaa
     3961 aaaggtgaga ctcttgcaat cgttggtgaa tctggttcag gaaaatctgt aactaccaga
     4021 actttaattg gtttaaatgc taaaaattca gagatatcgg ggaatgttca atttaagggg
     4081 cgtaaccttg ttgaactatc agaagaagag tggactaagg tacgcgggaa tgagatttct
     4141 atgattttcc aagaccctat gactagtttg gatccaacga tgaaaattgg tatgcaaata
     4201 gcggagccaa tgatgattca tcaaaaaatt tcaaaaaagg atgctttaaa attagctctt
     4261 gagctaatga aagatgtagg tatcccaaat gctgaagagc atattaatga ttacccacat
     4321 cagtggtctg gaggaatgcg ccaacgtgcg gttatagcga ttgctttagc tgctgatcct
     4381 gaaattctta ttgctgatga accaacgact gctttagatg taacaattca agcacaaatt
     4441 ttgaacttaa tgaaaaaaat tcaagcagaa cgagactcct ccattgtttt cataactcat
     4501 gacttagggg tcgttgcagg gatggcagac cgtgtagcag ttatgtatgc agggaaaatt
     4561 gttgaatttg ggactgttga tgaagtcttt tataatccac aacatccata tacttgggga
     4621 ttgttgaatt caatgccgac aaccgacaca gaatcaggta gtttagagtc aattccggga
     4681 acaccaccag atttgttgaa tccacctaaa ggagatgcat ttgcagctcg taatgaattt
     4741 gcattagata ttgatcatga agaagaacca ccgtacttca aagtttcaga aacacatttc
     4801 gctgctacat ggcttttaga tgaaagatca cctaaggtgc ttcctccatt accgatccaa
     4861 aaacgttggg aaaaatggaa cgagattgaa gggaggaaag cctaatgact gaaaatcgaa
     4921 aaaaattagt tgaagtcaaa aatgtttctt tgaccttcaa taaaggaaaa gctaatgaag
     4981 tgagagcaat tgataatgtt agttttgaca tttatgaagg tgaagtgttt ggattagttg
     5041 gggaatctgg atcaggaaag acaaccgttg gacgttcaat tttaaaactt tatgatattt
     5101 ctgatggaga aattaccttt aatggagaag tgatctcaca tctgaaaggt aaagcattac
     5161 atagttttcg taaagacgct cagatgattt ttcaggatcc tcaggctagc ttaaacggcc
     5221 gtatgaagat tcgtgatatc gtagcagaag gtttagatat acataaatta gctaaatcaa
     5281 aatcagatcg tgatagtaaa gttcaggccc tattagatct tgtagggtta aataaagatc
     5341 atttaacacg ttatccgcat gaattttcag gtggacaacg acaacgtatc gggattgcac
     5401 gtgctttagc agtagagcct aaatttatca ttgctgatga accaatatct gccttagatg
     5461 tttcaatcca agcacaagtt gttaatttaa tgcaaaaatt gcaaagagaa caaggattga
     5521 cttatttgtt tatcgcacat gatttgtcta tggtaaaata catttcagat cgtatcggtg
     5581 ttatgcattg gggaaaactg ttagaagttg ggacatctga tgatgtttat aacaatccaa
     5641 tccaccccta tacgaagagt ctattatcag ctattccaga accagatccg gagagtgaac
     5701 gtcaacgtgt tcaccagccg tataatccag ctattgagca agacggacaa gaacgtcaaa
     5761 tgcacgagat taccccttgt cattttgttt tatctacacc ccaagaagct gaagaatata
     5821 aaaaacaaat attataaaag atgggcta
//
LOCUS       DBJYSH010000112         5495 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.112, whole genome shotgun sequence.
ACCESSION   DBJYSH010000112 DBJYSH010000000
VERSION     DBJYSH010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 5495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5495
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.112"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            complement(<1..1190)
                     /gene="metK"
                     /locus_tag="ACUBV3_001734"
     CDS             complement(<1..1190)
                     /gene="metK"
                     /locus_tag="ACUBV3_001734"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HHU3848219.1"
                     /translation="MSERKLFTSESVSEGHPDKIADQISDAILDAILEQDPDAHVAAE
                     TAVYTGSVHVFGEISTTAYVDINRVVRNTIAEIGYDKAEYGFSAESVGVHPSLVEQSP
                     DIAQGVNEALEVRGSLEQDPLDLIGAGDQGLMFGFAVDETPELMPLPISLAHQLVKKL
                     TDLRKSGELTYLRPDAKSQVTVEYDDNDQPIRVDAVVISTQHDPNVTNDQLHKDVIEK
                     VINEVIPSHYLDDQTKFFINPTGRFVIGGPQGDSGLTGRKIIVDTYGGYSRHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAADLAKKVEVQLAYAIGVAQPVSVRVDTFGTGVIA
                     EADLEAAVRQIFDLRPAGIINMLDLKRPIYRQTAAYGHMGRTDIDLPWERVDKVQALK
                     DFIA"
     gene            1375..2310
                     /gene="birA"
                     /locus_tag="ACUBV3_001735"
     CDS             1375..2310
                     /gene="birA"
                     /locus_tag="ACUBV3_001735"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HHU3848220.1"
                     /translation="MKTYEKIYQILSKNNNFISGETMANQLNISRTAIWKGIKTLEEL
                     GLEIESVTNKGYRLVSGDILLPEQLEQEIGIKVSLNNNSASTQLDAKMGIESKLKTPH
                     LFLAPNQKKAKGRFDRPFFTSNQGGIYMSLLLQPNVPIEDIKPYTVMVASSAVKAISR
                     LTGITPEIKWVNDIYLDNKKIAGILTEAIASVESGLVTNVIIGLGINFYIKEFPRALT
                     KRAGSLFTEQPTITRNQLITEIWNLFFNIPLEDHLKVYREKSLVLDKTVSFMDGQTMY
                     SGKAIDITDKGYLVVELDDGQLKTLRSGEISLSSW"
     gene            complement(2291..2485)
                     /locus_tag="ACUBV3_001736"
     CDS             complement(2291..2485)
                     /locus_tag="ACUBV3_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3272 domain-containing protein"
                     /protein_id="HHU3848221.1"
                     /translation="MTKRQFIFMALLCSFETYFFNQSVMDGSWIFAIFWGVLLLRDLQ
                     KVYAISKFTKELIKSTKKKD"
     gene            complement(2574..4238)
                     /gene="dnaX"
                     /locus_tag="ACUBV3_001737"
     CDS             complement(2574..4238)
                     /gene="dnaX"
                     /locus_tag="ACUBV3_001737"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HHU3848222.1"
                     /translation="MYQALYRKYRSQTFDEMVGQSVISTTLKQAVSSKKISHAYLFSG
                     PRGTGKTSAAKIFAKAMNCPNQVNGEPCNHCDICRDITNGSLEDVIEIDAASNNGVDE
                     IRDIRDKSTYAPSRATYKVYIIDEVHMLSTGAFNALLKTLEEPTENVVFILATTELHK
                     IPATILSRVQRFEFKAIKLLAIRDHLAQILDKEAISYDLDALTLVARRAEGGMRDALS
                     ILDQALSLAKDNHISLDVAEEITGSISLSAIDDYVSNILAHDTTEALAKLEVIFDSGK
                     SMSRFATDLLMYLRDLLVVQAGGENSHSSDTFMANLDVKQDILFEMIDKVTSVLPEIK
                     NGSHPKVYAEMMTIQLSEMVEKNSSNIPADVTAELDSLRRELKSLKNEMSQLSRADQS
                     SSTQKVKVNNKTFTFKVDRTKILTIMEETVVDSQRSREYLEALKSAWNEILDNITAQD
                     RALLMGSEPVLANSENAILAFDAAFNAEQAMKRTDLNDIFGNIMSKAAGFSPNILAVP
                     RNDFNQIRSDFAKKMKAQKTETEPEVNHQIPEDFSYLAERIAIVED"
     gene            complement(4238..4735)
                     /locus_tag="ACUBV3_001738"
     CDS             complement(4238..4735)
                     /locus_tag="ACUBV3_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HHU3848223.1"
                     /translation="MNKSKKIENYQLLLLQAQALFSDETNALANLSNASAMLNAMLPN
                     SVFTGFYLFDGEELILGPFQGGVSCVHITLGKGVCGESAQTAKTLIVDDVTKHANYIS
                     CDSKAMSEIVVPMFKNGKLLGVLDLDSSLVADYDEIDQEYLEKFVGILVEHTIWNLDM
                     FGVEK"
     gene            complement(4822..5451)
                     /gene="udk"
                     /locus_tag="ACUBV3_001739"
     CDS             complement(4822..5451)
                     /gene="udk"
                     /locus_tag="ACUBV3_001739"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HHU3848224.1"
                     /translation="MRKKPIIIGVTGGSGGGKTSVSRAILSNFPDQKIIMIEHDSYYK
                     DQSHLTFEERVKTNYDHPLAFDTNLMIEQLNELIEGRPVDIPVYDYTKHTRSDRTIRQ
                     EPQDVIIVEGILVLEDQRLRDLMDIKLFVDTDDDIRIIRRIKRDMEERDRSLDSIIEQ
                     YTEVVKPMYHQFIEPTKRYADIVIPEGVSNIVAIDLINTKVASILNEAK"
BASE COUNT         1782 a         1072 c          880 g         1761 t
ORIGIN      
        1 ctagcaataa aatcctttaa ggcctggact ttatcaactc gctcccatgg caaatcgata
       61 tcagtacgtc ccatatgccc ataggctgct gtttgtcgat aaataggtcg tttgaggtcc
      121 aacatattaa taatgcctgc tggtcgcaaa tcaaatattt ggcgtacagc tgcctctagg
      181 tctgcttctg ctataacacc tgttccaaat gtatctacac gcacagagac aggctgagca
      241 acaccaattg cataagcaag ttgtacttct acttttttag ctaaatcagc tgcaacaata
      301 ttttttgcaa tataacgagc tgcataagat gctgaacgat ctactttagt agcatcttta
      361 cctgaaaatg caccaccacc atgacgagaa taaccaccat aagtatctac aataatctta
      421 cgacctgtta gtcctgaatc tccttgaggg cctccaataa caaagcgccc tgtaggattg
      481 ataaagaatt ttgtttggtc atctaaataa tggcttggaa taacttcatt gataactttc
      541 tcaatcacgt ccttatggag ttggtcatta gttacattag gatcatgctg tgtcgaaata
      601 actactgcat ccacacgaat tggttgatca ttatcatcat attcaaccgt tacttgagat
      661 tttgcatctg gtcgaagata ggtgagttcc ccagacttac gtagatctgt caatttctta
      721 actaactgat gagctaaaga aattggcaat ggcattaact caggtgtttc atcaacagca
      781 aacccaaaca tcaaaccttg gtcacctgca ccaattaaat ctaaaggatc ttgttcaaga
      841 gaaccacgta cctcaagagc ttcattgact ccctgtgcaa tgtctggcga ctgttcaacg
      901 agtgacggat gcactccgac agattcagct gagaagccat attcagcttt atcatagcca
      961 atttcagcga ttgtgttacg gacaactcgg ttaatatcaa cataggctgt cgttgatatc
     1021 tcgccaaata catgaaccga acctgtataa acagctgttt cggctgctac gtgggcatct
     1081 ggatcttgct ctaaaattgc atctaaaatc gcatcggaaa tttggtcagc aatcttatcg
     1141 ggatgtccct cagatactga ctcagacgtg aaaagtttac gttctgacat aaaaatgtcc
     1201 cccctaaaat gtaaaatgtg tcgagttaca aagaactagc ctctaggcta ccttttcggg
     1261 actcatatag ctattgtacc actatttcat cttttgtgaa ataagttacc aataaataaa
     1321 aaattatctc tattttcttt agtctttttt ctcgatttat actatactag atttatgaaa
     1381 acctacgaaa aaatttatca aatactatca aaaaataaca actttattag cggagaaact
     1441 atggcaaatc aacttaatat ttcccgcaca gctatttgga aaggtataaa aacgttagaa
     1501 gaattaggcc tagagattga gtctgtcact aacaagggct accgattggt atcaggtgac
     1561 atattattac cagaacaact cgaacaagaa attggtatta aggttagtct taataataat
     1621 agtgcttcaa cccaactaga tgctaaaatg gggatagaaa gcaaattaaa gactccacac
     1681 ctttttttag ctcctaatca aaaaaaagct aaaggacgtt ttgatagacc tttctttaca
     1741 tcaaatcaag gcggcatcta tatgtctctt ttattacaac ctaatgtacc gattgaagat
     1801 ataaaacctt atactgtcat ggtagcctct agtgctgtaa aagctatctc acggttaaca
     1861 ggaattactc ctgaaattaa gtgggtcaat gatatttatt tagataataa aaaaatagct
     1921 ggtattttaa ccgaggctat cgcttctgtt gagagtggat tggtaacgaa tgttattata
     1981 ggattaggta tcaatttcta tatcaaagag ttccctaggg cgttaactaa gcgcgcaggt
     2041 agtctcttta cagagcaacc gaccataact agaaatcaat tgattacaga aatctggaat
     2101 ctctttttca atattccact agaggatcac ttaaaggtat atagagaaaa atccctagtc
     2161 ttagataaaa ccgttagctt tatggatggt caaacaatgt attctgggaa agctattgat
     2221 attactgata aaggatattt agtggttgag ttagatgatg gtcagttgaa aacacttcgc
     2281 agtggcgaaa tcagtctttc ttcttggtag atttgataag ctcttttgtg aattttgaaa
     2341 tcgcatacac cttttgaaga tctcgaagta ataaaactcc ccaaaaaata gcaaaaatcc
     2401 aggagccatc cataacggat tgattgaaaa aataagtttc aaaagagcat aaaagcgcca
     2461 taaaaataaa ttgtcgtttt gtcatatcac cattataaca gaatctacta aataattaaa
     2521 aaacttatca aaaaaatagt tgcctgttac gggcaactat ttttagttac gttttaatct
     2581 tcaacgattg ctattcgttc tgctagataa gaaaagtctt ctggaatctg atgattaact
     2641 tcaggttctg tttccgtctt ttgagctttc attttctttg caaaatcgct ccgaatttga
     2701 ttaaaatcat ttctaggtac agctaaaata ttaggtgaaa aacctgctgc tttactcata
     2761 atattaccaa atatatcatt gagatcggta cgtttcatag cttgttcagc attaaaggcc
     2821 gcatcaaatg ccaaaatagc attctcgcta ttagctaaga caggttcaga tcccattaat
     2881 aaggctctat cttgagccgt aatattatct aatatctcgt tccaagcact ctttaaagct
     2941 tctaaatatt cacgtgaacg ttggctatct acaaccgttt cttccataat ggtcaaaatc
     3001 tttgtacggt caactttgaa tgtgaatgtc ttattattaa cctttacttt ttgagttgat
     3061 gaactttgat ctgctcgaga gagttggctc atttcgtttt ttaacgattt taactctcga
     3121 cgaagactat ctaattctgc tgtcacatct gcgggtatgt ttgacgaatt tttttctacc
     3181 atttcagaca attgaatggt catcatctct gcataaactt taggatggct accatttttt
     3241 atctcaggaa gaacagatgt cactttatca atcatttcaa ataaaatatc ttgtttaaca
     3301 tcaagattgg ccataaaagt atcacttgaa tgactatttt caccacctgc ttgaacaaca
     3361 agtaaatctc ttaagtacat caataaatct gttgcaaaac gactcatact tttgccacta
     3421 tcaaagatta cttcaagttt agcgagtgct tcagttgtat cgtgagccaa tatattcgat
     3481 acataatcat ctatagcaga taaggaaatg ctccctgtaa tttcttctgc tacatctaaa
     3541 gaaatgtggt tatctttcgc taaactcaag gcttgatcga gaattgataa agcatcacgc
     3601 ataccacctt cggctcgtct agcgactagt gtcagagcat ccaaatcata tgaaatagct
     3661 tctttatcta aaatctgtgc taaatgatct cgaatagcta ataatttaat cgctttaaat
     3721 tcaaagcgct ggacacgaga taagattgtt gcagggattt tatggagttc tgttgttgct
     3781 aatataaaaa caacattttc tgttggctct tcaagtgttt tcaatagtgc attgaaggca
     3841 cctgtagaca acatatgaac ttcatcaata atataaactt tataggttgc cctacttgga
     3901 gcataagttg atttgtccct gatatcacga atttcatcga caccattatt tgacgcagca
     3961 tcaatttcaa tgacatcctc taaactacca tttgtaatat ctcgacaaat atcacaatga
     4021 ttacaaggtt cgccattaac ttgatttggg caattcatcg cttttgcaaa tattttagca
     4081 gcacttgttt ttccagttcc cctaggtcct gaaaaaaggt aagcatggct aatttttttg
     4141 ctggacacag cttgctttaa agtagttgaa ataacacttt gtccaaccat ttcatcaaat
     4201 gtttggctac gatatttccg ataaagggcc tgatacatta cttttcaact ccaaacatat
     4261 ccaaattcca aatcgtatgt tctactagaa tacctacaaa tttttctaag tattcttgat
     4321 caatctcatc ataatctgct actaaagaag aatctaaatc tagaactcct agaagtttgc
     4381 catttttaaa catgggtact acgatttcac tcatagcttt tgaatcacag gagatatagt
     4441 tagcatgctt tgtaacatca tcaacaatca gcgtcttagc agtttgtgca gattcaccac
     4501 aaacaccttt tcctaaagta atatgcacac atgatacacc accctgaaaa gggccaagaa
     4561 ttaactcctc tccatcaaat aaataaaagc ctgtaaatac agaatttgga agcatagcat
     4621 ttagcatagc tgaagcattt gataagttgg caagagcatt tgtttcatct gagaatagag
     4681 cttgcgcttg tagtaataat aattgataat tttcgatttt cttagacttg ttcatagcac
     4741 ttattatagc ataatttgaa agttactaaa actaaataac catcctatat aaaacaaaaa
     4801 aataaacaca aaccattgtg tttattttgc ttcatttaag atgctagcca ctttggtatt
     4861 gattaaatca atagcgacaa tattacttac tccttctgga attacaatat ccgcataacg
     4921 ctttgttggt tcgataaatt gatgatacat aggttttaca acttctgtat actgttcaat
     4981 aatactatct aaacttctat cgcgctcttc catatcacgc ttaatacgac gaataatacg
     5041 aatatcatca tctgtatcaa caaataattt gatatccatt aaatcacgca aacgctgatc
     5101 ttctaatacc aaaattccct caacaataat gacatcctga ggttcttggc ggatagttct
     5161 atcactacgg gtatgttttg tgtaatcata aaccggaata tcgaccggac gcccctcaat
     5221 caattcgttc aattgctcaa tcatcaaatt ggtatcaaaa gcaaggggat gatcataatt
     5281 ggtttttaca cgttcttcaa aagtcaaatg actttgatct ttatagtaag aatcatgttc
     5341 aatcatgata attttttgat ctgggaaatt tgataaaatc gcccgagaaa cactggtttt
     5401 accaccacca gatccacctg taacaccaat aataataggt tttttacgca tttgtcactc
     5461 caaattaaat ttatcctcta tctattgtac tataa
//
LOCUS       DBJYSH010000113         5406 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.113, whole genome shotgun sequence.
ACCESSION   DBJYSH010000113 DBJYSH010000000
VERSION     DBJYSH010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 5406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5406
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.113"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            complement(<1..789)
                     /locus_tag="ACUBV3_001740"
     CDS             complement(<1..789)
                     /locus_tag="ACUBV3_001740"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HHU3848225.1"
                     /translation="MKKIIYLGLACVSILTLSGCESIERSLKGDRYVDQKLAENSSKE
                     ATEQLNKKTKQALKADKKAFPQLDKAVAKNEAQVLIKTSKGDINIKLFPKYAPLAVEN
                     FLTHAKEGYYNGLSFHRVIKDFMIQSGDPNGDGTGGKSIWNSKDKKKDSGNGFVNEIS
                     PYLYNIRGSLAMANAGADTNGSQFFINQSQQDHSKQLSDKKVPKVIIKAYSEGGNPSL
                     DGGYTVFGQVISGMETVDKIASVEVTKSDQPKEKITITSIKVIKD"
     gene            955..3405
                     /locus_tag="ACUBV3_001741"
     CDS             955..3405
                     /locus_tag="ACUBV3_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000231573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HHU3848226.1"
                     /translation="MVFMANKKKTKGKKTRRPTKAEIERQRAIQRMITALVLTIILFF
                     GIIRLGIFGITVYNVIRFMVGSLAYLFIAATLIYLYFFKWLRKKDSLVAGFLIASLGL
                     LIEWHAYLFSMPILKDKEILRSTARLIVSDLMQFKITVFAGGGMLGALIYKPIAFLFS
                     NIGAYMIGVLFIILGLFLMSSLEVYDIVEFIRAFKNKVAEKHEQNKKERFAKREMKKA
                     IAEQERIERQKAEEEAYLASVNVDPETGEILEDQAEDNLDDALPPEVSETSTPVFEPE
                     ILAYETSPQNDPLPVEPTIYLEDYDSPIPNMRENDEEMVYDLDDDVDDSDIENVDFTP
                     KTTLVYKLPTIDLFAPDKPKNQSKEKDLVRKNIRVLEETFRSFGIDVKVERAEIGPSV
                     TKYEIKPAVGVRVNRISNLSDDLALALAAKDVRIEAPIPGKSLIGIEVPNSEIATVSF
                     RELWEQSDANPENLLEVPLGKAVNGNARSFNLARMPHLLVAGSTGSGKSVAVNGIISS
                     ILMKARPDQVKFMMIDPKMVELSVYNDIPHLLIPVVTNPRKASKALQKVVDEMENRYE
                     LFSKIGVRNIAGYNTKVEEFNASSEQKQIPLPLIVVIVDELADLMMVASKEVEDAIIR
                     LGQKARAAGIHMILATQRPSVDVISGLIKANVPSRIAFAVSSGTDSRTILDENGAEKL
                     LGRGDMLFKPIDENHPVRLQGSFISDDDVERIVGFIKDQAEADYDDAFDPGEVSETDN
                     GSGGGGGVPESDPLFEEAKGLVLETQKASASMIQRRLSVGFNRATRLMEELEAAGVIG
                     PAEGTKPRKVLMTPTPSE"
     gene            3487..5202
                     /gene="pabB"
                     /locus_tag="ACUBV3_001742"
     CDS             3487..5202
                     /gene="pabB"
                     /locus_tag="ACUBV3_001742"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008153 - 4-aminobenzoate biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HHU3848227.1"
                     /translation="MHIETVIDFKELGKRYRFKNPTKELIADTLEQVLEVIKEVDYYQ
                     SQNYYVVGYLSYEASAAFDSHFKVSQQKLAGEHLAYFTVHKDCENEAFPLSYENVRLA
                     DNWTANVSEQEYQEAIANIKGQIRQGNTYQVNYTLELSQQLCSDPFSVYERLMVEQGA
                     GYNAYIAYDDKRILSVSPELFFKKKDEVLTTRPMKGTSARKPTYQEDVAERDWLANDP
                     KNRSENMMIVDLLRNDMGRICDVGTVKVKKLCQVEQYATVWQMTSTIEGVLSPEVTLM
                     SIFQALYPCGSITGAPKISTMAIINELEKRPRGIYCGTIGLCMPDGQAIFNVPIRTVQ
                     MKGQQAYYGVGGGITWESQTDSEYEETRQKSAVLTRVNPKFQLITTGRVTENKLLFSQ
                     QHVERLVESASYFAYSFDKSKFERELKKYLHQLDEKDYRLKIMLDKTGKVTFEVKQLV
                     NLSKKFLTAEVVVQDYPIKLSPFTYFKTSYRPHIIEGQNEKIFVSPEGLLLETSIGNI
                     VLEKNGRFLTPDLSEGGLNGIYRRHLLKNQKVIEAPLTLKDLESADAIYACNAVRGLY
                     PLNLK"
     gene            complement(5251..>5406)
                     /locus_tag="ACUBV3_001743"
     CDS             complement(5251..>5406)
                     /locus_tag="ACUBV3_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HHU3848228.1"
                     /translation="SEMFYGLEKSYKSLDDLEQAITDYIFYYNNKRIKAKLKGLSPVQ
                     YRTKSFT"
BASE COUNT         1745 a          847 c         1088 g         1726 t
ORIGIN      
        1 gtctttaata actttgatac ttgtaatagt aattttttct tttggttgat ctgattttgt
       61 aacttctacc gaagcaattt tatccacagt ttccatgcca gagattactt gtccaaagac
      121 ggtataacca ccatctaaac ttggatttcc tccctctgaa taggctttaa taatcacctt
      181 aggaactttt ttatctgaca gttgtttgga atgatcttgc tggctttgat tgataaagaa
      241 ttggctgcca ttagtatcag ctccagcatt tgccattgct aaacttcccc tgatattgta
      301 gaggtaaggg gatatttcat taacaaaacc atttccagaa tctttctttt tatctttgct
      361 attccatata gattttccac cggtcccgtc tccattaggg tcaccagatt gtatcataaa
      421 atctttgata actctgtgga aacttaaccc attatagtat ccttcttttg catgtgttag
      481 aaaattttca acagctaaag gagcatattt tggaaataac ttgatattta tatcaccttt
      541 agaggttttt ataagcactt gtgcctcgtt tttggcaaca gccttgtcca attgaggaaa
      601 agctttttta tcagctttta aggcttgctt agtttttttg tttagttgtt cagtagcttc
      661 ctttgaagag ttttcagcta gtttttggtc aacataacgg tctcctttta gagaacgctc
      721 aattgactcg caaccactta aagttaatat actgacacag gctaaaccaa gataaataat
      781 ttttttcata catactcctt gattaagtta ttaagatgat tatattttat cataaatttt
      841 taaataattt ataaaagtaa atagtaaacc tattggggga cttgttgaaa ttatgataga
      901 atagtatagc atgagttgat agagaaagtg aggaacaagt atttcacttg aaatatggta
      961 tttatggcaa ataagaaaaa aacaaaagga aagaaaacca gaagacctac taaggcagaa
     1021 atagagcgtc aaagagctat tcaaaggatg attactgctc ttgttttaac aattattctc
     1081 ttctttggta ttatcagatt aggtattttt ggtattacag tctataacgt catccgtttt
     1141 atggtaggta gcttggctta cttatttatt gcggcaactt taatctacct ttatttcttt
     1201 aaatggttgc gaaagaaaga tagcttagta gcaggttttt tgatagcttc tttaggatta
     1261 ttgattgagt ggcatgctta ccttttctca atgcctattt tgaaagataa agaaattttg
     1321 cgttcaactg ctcgattaat tgtgtctgat ttaatgcaat ttaaaatcac tgtttttgcc
     1381 ggtggaggta tgttgggtgc tttgatttac aagccaattg cttttctctt ttctaatatt
     1441 ggtgcctata tgattggtgt tctcttcatc attttgggtc tctttttaat gagttctctg
     1501 gaagtttatg acatcgtcga atttattaga gcttttaaaa ataaagtggc agagaagcac
     1561 gagcaaaata aaaaggagcg ttttgctaag cgagagatga aaaaagcaat cgctgaacaa
     1621 gagcgcatag agcgtcaaaa agctgaagaa gaagcttatt tagcttcggt taatgtagac
     1681 cctgaaacgg gtgagattct agaggatcaa gctgaggaca atttggatga tgcgctacca
     1741 cctgaggtaa gtgaaacatc aactccggta tttgagccag agatccttgc ttatgagaca
     1801 tcgcctcaaa atgatccttt accagtagag ccgacaattt atttagaaga ctatgattcg
     1861 ccgattccta atatgagaga aaatgatgag gaaatggttt atgatttaga tgatgatgta
     1921 gatgatagtg atatagaaaa tgtcgacttt acacctaaaa cgacactggt ttataaatta
     1981 ccaacgatag atttatttgc accagataag cctaaaaatc aatccaaaga aaaggattta
     2041 gtccgaaaga atatcagagt tttagaagaa acatttagaa gttttggtat cgatgtaaaa
     2101 gtagaacgtg ctgaaattgg accatcagtt actaaatatg aaattaaacc agcagttgga
     2161 gttcgtgtga atcgtatttc aaatctatct gacgacctag ctcttgctct tgcagcaaaa
     2221 gatgtgcgta tagaagcacc aattcctgga aaatcattaa taggtattga agttcctaac
     2281 tcagaaattg caacggtttc tttccgcgaa ctttgggaac aatctgatgc caatcctgaa
     2341 aaccttttag aagtaccact aggaaaagct gttaacggca atgctcgcag ttttaactta
     2401 gctagaatgc cgcatctttt ggtagctggt tcaactggtt caggtaaatc tgtggcagtt
     2461 aatggaatta tttcaagtat tttgatgaag gcacgtccag atcaagttaa gtttatgatg
     2521 attgatccca aaatggttga attatctgtt tataatgata ttccacattt attaatccct
     2581 gttgtaacca atccgcgtaa agcaagtaag gcactccaaa aagttgttga tgaaatggaa
     2641 aatcgatacg agttatttag caaaattggt gtgcgtaata tagcaggtta taatacaaag
     2701 gttgaagagt ttaatgcttc ctctgagcaa aaacaaatac ctttgccttt aatcgttgtc
     2761 attgtagatg aattggctga cttgatgatg gttgctagta aagaagttga agatgctatt
     2821 attcgtttgg ggcaaaaagc acgtgctgca ggtatccata tgattcttgc aactcaacgt
     2881 ccatccgtag atgttatttc tggtttgatt aaagcaaatg ttccgtcgcg tattgcattt
     2941 gctgtttcaa gtggtactga tagccgtacg atccttgatg aaaatggtgc tgaaaagctc
     3001 ttgggacggg gtgacatgct ctttaagcct attgatgaga atcatccagt acgactacaa
     3061 ggttccttta tttcagatga tgatgttgaa aggatcgttg gttttatcaa agaccaagcc
     3121 gaggctgact atgatgatgc ctttgatcct ggagaagtat ctgaaacaga taacggctct
     3181 ggtggtggcg gcggagtacc tgaaagtgat cctctttttg aagaagccaa gggactcgtt
     3241 ttagagacgc aaaaagcaag tgcctcaatg attcaacgcc gattgtctgt tggtttcaat
     3301 agagcaacaa gactaatgga agaattagaa gcagcggggg ttattggtcc agcagaagga
     3361 accaagccac gaaaagtttt aatgactcca actccgagtg aataagaaaa aactaatctg
     3421 acaagaaatt gtcggattag ttttttatgt tataatgaag caaatagatg aagaagaagg
     3481 ttacgaatgc atattgagac tgttattgat ttcaaagaat taggaaaaag atatcgtttt
     3541 aaaaatccta caaaagaatt aatagctgat actttagaac aagtcttaga agtgataaaa
     3601 gaagttgatt attatcaatc tcaaaattat tatgttgttg gttatttatc ttatgaagca
     3661 tctgctgctt ttgattcaca ttttaaagtt tctcaacaga agttggctgg agaacatcta
     3721 gcttatttta cagtacataa agattgtgag aacgaagctt ttcctttaag ttatgaaaat
     3781 gttagattag cagataattg gactgctaat gtttctgagc aagaatatca agaggcaatt
     3841 gctaatatta aaggacaaat tagacaagga aatacttatc aagtaaatta tacactagag
     3901 cttagccaac aattatgctc ggatcccttt agtgtttatg aacgcctaat ggtagaacaa
     3961 ggagcaggat ataatgctta tattgcctac gacgataaaa ggattttatc tgtaagcccg
     4021 gaattgttct ttaagaagaa ggatgaagtc ttaacaacta gaccaatgaa gggaacaagc
     4081 gctagaaagc ctacttatca agaagatgtg gcggagcgag attggttggc aaatgatcct
     4141 aaaaatcgtt ctgaaaatat gatgattgtt gatttattaa gaaacgatat gggccgtatc
     4201 tgtgatgttg gaacagttaa ggtaaaaaaa ctatgtcaag tggagcagta tgcaactgtg
     4261 tggcaaatga catcaactat tgaaggagtt ttatcgccag aagtgacact tatgtctatt
     4321 tttcaagctc tatatccttg tggatctatc actggagctc ctaagatatc aacaatggct
     4381 attattaatg agttggaaaa acggccaaga ggtatttatt gtgggacgat aggactttgc
     4441 atgccagacg gacaagctat ttttaacgtt cctattcgca cagtacaaat gaaaggtcaa
     4501 caagcctatt acggcgttgg tggaggtatt acgtgggaga gccagacaga ttctgaatat
     4561 gaagaaaccc gtcaaaaatc agctgtttta acacgtgtca atccaaaatt tcaattaata
     4621 actacaggaa gagtgactga aaataaactt cttttctctc aacaacatgt cgagagatta
     4681 gttgaatcag cgtcttattt tgcttattct tttgataaaa gtaaattcga aagggaactg
     4741 aaaaaatacc ttcatcagct agatgagaag gattaccgct tgaaaataat gcttgataaa
     4801 actggtaagg taacgtttga ggtaaaacaa ctagtgaatt tatcaaaaaa gtttttaacg
     4861 gcagaggtgg tcgtacaaga ctaccctatt aaattatccc cgtttactta ttttaaaact
     4921 tcttatcgcc cacatattat tgaaggtcag aatgaaaaga tatttgtatc tcctgagggg
     4981 ttgctattgg aaacaagtat tgggaatatt gttttagaaa aaaatggaag gtttttaacc
     5041 ccagatttat cagaaggagg gttgaatggg atttatcgtc gtcatctcct taaaaaccaa
     5101 aaagtaattg aagcaccact aactttaaaa gatttagaat cagccgatgc tatatacgcc
     5161 tgtaatgctg ttagagggct ttatcctcta aacctaaagt aaaaagagtt ggaaattttg
     5221 tactgacccc caaaagttgg acaatttatt ttaagtgaag gatttagttc tgtattgcac
     5281 aggactaagt ccttttagtt ttgctttgat tcgcttgttg ttgtagtaaa aaatgtaatc
     5341 tgtgatagct tgctcaaggt catcaagtga tttgtaagac ttctccaagc catagaacat
     5401 ctcaga
//
LOCUS       DBJYSH010000114         5373 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.114, whole genome shotgun sequence.
ACCESSION   DBJYSH010000114 DBJYSH010000000
VERSION     DBJYSH010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 5373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5373
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.114"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            26..952
                     /locus_tag="ACUBV3_001744"
     CDS             26..952
                     /locus_tag="ACUBV3_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012997850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HHU3848229.1"
                     /translation="MEKIILTATAESIEQVKQLLAIGIDRIYVGEENYGLRLPHSFSD
                     DELREIAKLVHDAGKELTVACNALMHQEMMDNIKPFLELMKEINVDYLVVGDAGVFYI
                     NKRDGYNFKLIYDTSVFVTSSRQVNFWGQHGAVETVLAREIPSEELFKMSENLEFPAE
                     ILVYGASVIHHSKRPLLQNYYNFTHITDEKTRERGLFLAEPGDPESHYSIYEDKHGTH
                     IFINNDINMMTKVTELVEHHFTHWKLDGIYCPGDNFVAIAEIFVETARLIENGTFTQD
                     QAFLFDERIRKLHPKGRGLDTGFYDFDPSTVK"
     gene            1082..2368
                     /locus_tag="ACUBV3_001745"
     CDS             1082..2368
                     /locus_tag="ACUBV3_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HHU3848230.1"
                     /translation="MSNVKKRPEVLSPAGTLEKLKVAIDYGADAVFVGGQAYGLRSRA
                     GNFSMEELQEGINYAHARDAKVYVAANMVTHEGNELGAGPWFRELRDMGLDAVIVSDP
                     ALIVICATEAPGLEIHLSTQASSTNYETFEFWKEMGLTRVVLAREVTMAELAEIRKRT
                     DVEIEAFVHGAMCISYSGRCVLSNHMSHRDANRGGCSQSCRWKYDLYDMPFGQERQSL
                     KGEIPEPFSMSAVDMCMIEHIPDMIENGVDSLKIEGRMKSIHYVSTVTNCYKAAVDAY
                     MESPEAFEAIKEDLIDELWKVAQRELATGFYYHTPTENEQLFGARRKIPQYKFVGEVV
                     SFDNAKMEATIRQRNVIMEGDRVEFYGPGFRHFECFIEGLRDAEGNKIDRAPNPMELL
                     TITLPNPVKKGDMIRACKEGLVNLYQNDGTSKTIRA"
     gene            2495..2707
                     /locus_tag="ACUBV3_001746"
     CDS             2495..2707
                     /locus_tag="ACUBV3_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HHU3848231.1"
                     /translation="MSEFTFEIVEKLLVLSENEKGWTKELNRVSFNGAPAKFDLRTWS
                     PDHTKMGKGITLSNEEFKVILDAFRK"
     gene            2831..3628
                     /locus_tag="ACUBV3_001747"
     CDS             2831..3628
                     /locus_tag="ACUBV3_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000618018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HHU3848232.1"
                     /translation="MIRHSFKSKMLLLVFILTIIVNYLSATGLLTGNSQKSLSDRYQT
                     LLTPAPLAFSIWSVIYLLTFLVILRAIFSKSQSYQDNFASIFPYFLGLLLVNNIWTVF
                     FTSNLIGLSTIIIFAYCILLVIIIKILSKNKSKLLLRITFGIHAGWLLVASLVNLAVY
                     LVKIDFNYPLPKVYIAIIALIFITVLSLYLARVLQNAYLILSVFWAWLMVFKAHLEGN
                     FQHLYFSFQILSLIAAIILGVSFFIIGYYNVKERYKQKRTSPYSRVL"
     gene            complement(3730..5070)
                     /locus_tag="ACUBV3_001748"
     CDS             complement(3730..5070)
                     /locus_tag="ACUBV3_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001073016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HHU3848233.1"
                     /translation="MNQNHSVKRSLSEVNQSVEVPHNSSFWNTLRAFLGPGALVAVGY
                     MDPGNWITSVIGGATYRYLLLFVVLVSSLMAMQLQQMAGKLGIVTRQDLAQATASRLP
                     KPLRYLLFIIIELALIATDLAEVIGSAIALHLLFGWPLLLSIMITILDVFLLLLLMKL
                     GVQKIEAFVSVLILTILIIFTYLVVLSQPDLDAMFKGFLPHHELFNISHEGKNSPLTL
                     ALGIIGATVMPHNLYLHSSLSQTRRVDYHNKSSIKKAVRFMTLDSNIQLSLAFVVNSL
                     LLVLGASLFYGHANDISAFSQMYLALSDKTITGAVASSFLSTLFAVALLASGQNSTIT
                     GTLTGQIVMEGFLHFKLPQWLIRLCTRLLTLLPIFAIALLVGGEENTLDQLIVYSQVF
                     LSLALPFSIFPLIYFTSQKSIMGEHANTKWNTYLAYLIAIILTLLNLKLIMDLL"
BASE COUNT         1817 a          797 c         1042 g         1717 t
ORIGIN      
        1 ctgaaagttt gttataatac taattatgga aaaaataatt ttgacagcga cagctgaatc
       61 tattgaacaa gtaaagcaat tgctagcaat aggaattgac cgcatctacg taggggaaga
      121 aaattacggt cttcgtttac cacattcatt ttcagacgat gaattgcgtg aaatcgctaa
      181 attggtacat gatgctggta aagaattgac agtagcttgt aatgcactca tgcatcaaga
      241 aatgatggat aatatcaaac catttttgga gttgatgaaa gaaattaatg tggattattt
      301 ggtagttggt gatgcaggag tattttatat caacaagcgt gatggttata acttcaaact
      361 tatctacgac acttctgtat tcgttacatc aagtcgtcaa gttaattttt ggggacaaca
      421 tggagcagta gaaacggttt tagcacgtga aataccttct gaagaacttt ttaagatgtc
      481 tgagaacctt gaatttcctg ctgagatttt agtttatggg gcttctgtaa ttcatcactc
      541 taagcgtccg ttattgcaaa actattataa ttttactcat atcactgatg aaaaaacacg
      601 tgagcgtggt ctttttttag cagaaccagg tgatccagaa agtcactatt ctatttacga
      661 agataagcat gggactcata tttttattaa caatgatatc aatatgatga ctaaagtgac
      721 tgaactagtt gaacatcact ttactcactg gaaattagat ggtatttatt gtccaggaga
      781 taattttgtt gctattgctg aaatttttgt cgaaacagct cgtttgattg aaaatggaac
      841 attcactcag gatcaggctt tcttgtttga tgaacgaatt agaaaactgc accctaaagg
      901 acgtggttta gatactggat tttatgattt tgacccatca acagtaaaat aagatttatt
      961 tgctacgtga aagtttttta tctcaaagcg tagccaattg cactaagaac tgcatatcga
     1021 ttggtatgtt aattaagata gaaaataatt cttttatctt gaaaatagaa ggaaatataa
     1081 gatgtctaat gtaaaaaaac gccctgaggt tttatcacct gcaggaacac tagaaaaatt
     1141 aaaagttgct attgattatg gagcagatgc tgtatttgtt ggaggtcaag cgtatggtct
     1201 tcgaagtaga gctggtaact tttctatgga agagttacaa gagggaataa actatgctca
     1261 tgcgagagat gctaaagttt atgtagcggc taatatggtt actcatgaag gtaatgagct
     1321 tggggcaggt ccgtggtttc gtgaattacg tgatatggga ctagatgcag tcattgtttc
     1381 agatcctgct cttattgtta tttgtgctac agaagcacca ggtttagaaa ttcatttgtc
     1441 aactcaagcc tcttccacga actatgaaac ttttgaattt tggaaagaga tggggcttac
     1501 tcgtgtcgta ttagcacgtg aggttactat ggcagagttg gctgaaatca ggaagaggac
     1561 agatgttgag atagaagcat ttgttcatgg cgcgatgtgt atttcatact caggacgatg
     1621 tgttctatca aaccatatga gccatcgtga tgctaatcgt ggcggttgct ctcagtcatg
     1681 tcgttggaaa tatgacctct acgatatgcc atttggacaa gaacgtcaat cgttaaaagg
     1741 cgagattcca gaacctttct caatgtcagc tgtggatatg tgtatgattg agcatattcc
     1801 agatatgatt gaaaatggcg tagatagttt aaaaatagaa ggacgtatga aatccattca
     1861 ttatgtttct acagtaacta attgctataa agctgctgta gatgcctata tggaaagtcc
     1921 agaagctttt gaagctatta aagaagactt gattgatgaa ctttggaagg ttgcacaacg
     1981 cgaattagca acaggttttt actaccatac accaactgaa aacgaacaac tctttggagc
     2041 tcgtcgtaaa attcctcaat acaaatttgt tggggaagtg gtttcatttg acaatgctaa
     2101 aatggaggct acaattcgtc agcgtaatgt tattatggaa ggagatcgcg tagaattcta
     2161 tggtcctggc ttccgtcact ttgaatgttt tattgaaggt ctgcgcgatg ctgaaggaaa
     2221 taaaatagac cgtgctccaa atccgatgga attattaacc ataacattac caaatccagt
     2281 aaaaaaaggg gatatgattc gtgcttgtaa agaaggatta gtgaaccttt atcaaaatga
     2341 tggtactagc aagactataa gagcttagat acttttataa ctattttgtc attttacatg
     2401 gtcaatatta ctaaaaattg ttaaaataga agaagttcat ttaaatagaa caaaaagcta
     2461 taaaaataac gaatagtata agtggaggta taaaatgtca gaatttacat ttgaaattgt
     2521 tgagaagtta ttggtcctat ctgaaaatga aaaaggttgg acaaaagagt tgaatcgtgt
     2581 tagtttcaac ggagcgccag ctaaatttga tttgcgtaca tggagtccag atcatacaaa
     2641 aatgggtaaa ggtatcactt tatctaatga agaatttaaa gtcattttag atgcttttcg
     2701 taaataaata aaaagtggtt tcaaaaacca ctttttttgt cgagacaagc gagtattcag
     2761 taaacatgtt gattaaaaaa atagaatttt taaactaaaa gaattataat ggaggtaaaa
     2821 ggagtcctct atgattagac attcatttaa aagtaagatg ttattgttag tctttatctt
     2881 aacaataata gtgaattatt tatccgcaac aggtttgtta acagggaaca gtcaaaagag
     2941 tctgtctgat cgttatcaaa cgctcttgac accagcacca ttagcctttt caatttggtc
     3001 agtcatctat ttattgacct ttttagtaat attaagagct atattcagta agagtcaatc
     3061 ttaccaagat aactttgcta gcatttttcc atactttttg ggtcttctat tagttaataa
     3121 tatatggact gtatttttta cgagcaattt gattggccta tcaacaataa taatttttgc
     3181 ttactgtatt cttctggtta tcattatcaa gatactaagt aaaaataaat caaaattact
     3241 gcttagaata acatttggaa ttcatgcagg atggttgcta gttgcgtcat tggtcaattt
     3301 agcggtctac ttagttaaaa ttgattttaa ttacccatta ccaaaggtat atatagctat
     3361 cattgccttg atttttataa ctgttttaag tctttacctg gctagggtat tacaaaatgc
     3421 ttacttaatc ttatccgttt tttgggcttg gttgatggtt tttaaagcac atttagaagg
     3481 aaattttcaa cacctatatt ttagtttcca aattttatct ttgattgcag caattatcct
     3541 tggcgtatcg tttttcatca tcggttatta taatgtcaag gagaggtaca agcaaaaacg
     3601 tactagtcct tactcacggg ttctttaaaa gaaatacaaa aagctccatc attatgtact
     3661 ttcctctatc attagatgta tgtctcatga tagaggttca gttcattagt ggggcttttt
     3721 tgtattgtat taaagtaaat ccatgattaa tttcaaattg agtaatgtta aaatgatagc
     3781 gataaggtat gctaaatagg tattccattt agtattagca tgctctccca taattgattt
     3841 ttgcgatgtg aaataaatca atggaaagat tgaaaatggt aaagctaagg aaagaaaaac
     3901 ttgtgaatag acgattagtt gatctaacgt attttcttcg cctccaacta gtagggcgat
     3961 agcaaaaatt ggaagaagag ttaataatct agtacaaagc cttattaacc attgaggtaa
     4021 tttgaagtga aggaaacctt ccataacgat ttgtcctgtt aaagtacctg taattgttga
     4081 attttgacca cttgctagta gggcaactgc aaaaagggta gaaaggaaag aactggctac
     4141 tgcaccagta atagttttat cagataaggc aagatacatt tgagaaaagg cactgatatc
     4201 attagcatgc ccataaaata gactagctcc taaaactaat agtagcgaat tcacgacaaa
     4261 tgcaagtgat agctgaatgt tagaatctaa ggtcatgaaa cgtacagctt tttttattga
     4321 agatttatta tgataatcca cgcgtctagt ctgagataaa gaagaatgta aataaagatt
     4381 atgtggcatg actgttgcgc cgataatgcc aagagcgagg gttaaaggac tatttttccc
     4441 ttcatgtgat atgttaaaaa gctcatggtg tggtaaaaat cctttgaaca tagcatctaa
     4501 atcaggttgt gataaaacaa ctaaatatgt aaagattatc aaaattgtca atatgagaac
     4561 agaaacaaag gcttcaattt tttggacgcc taacttcatt aagagtaaaa gtaagaatac
     4621 atccaaaata gtaatcatga tagacagtaa caggggccag ccaaataata agtgaagagc
     4681 aatggcagag ccaatgactt ctgctaaatc agtagcgata agggctaact caatgataat
     4741 aaatagcagg taacgaagcg gttttgggag tcgagaagct gttgcttgtg ctaagtcctg
     4801 tcgtgtaaca ataccaagct tgccagccat ttgttgtaat tgcatcgcca tcaaagaaga
     4861 tactaaaaca acaaacaata gtaagtaacg ataggtagct ccaccaataa cgctagtaat
     4921 ccagttacct ggatccatat aaccaactgc aaccaaagct ccaggtccaa gaaaagctct
     4981 taaagtgttc caaaaagagc tattgtgagg aacctcaacg ctctgattaa cttcggataa
     5041 ggatcttttg acggaatgat tttgattcat gtaatactat actttctagt aatctcaagc
     5101 cattataaca aatagaaaat agaaaaacaa ttaaaaatta atgctactta ataatcgatt
     5161 gaggtgctgt ttagtattag agaactattt tcttaacagt tacatttgaa ataacaaaat
     5221 aacagatttc tatcgattat ttttagttcc gtagatttac ctaagttatt taagtggagt
     5281 aaactatatc tgtagtgaat tttttaagaa atataatcca ataatactat cgattcttgt
     5341 ttgattagtt cgaaagattg ctttatggta tag
//
LOCUS       DBJYSH010000115         5364 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.115, whole genome shotgun sequence.
ACCESSION   DBJYSH010000115 DBJYSH010000000
VERSION     DBJYSH010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 5364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5364
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.115"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            84..1331
                     /locus_tag="ACUBV3_001749"
     CDS             84..1331
                     /locus_tag="ACUBV3_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HHU3848234.1"
                     /translation="MEKNTWKKLLVSTAALSVVAGGAIAATHSNSVDAASKTTIKLWV
                     PTDSKASYKAIVKKFEKENKGVTVKMIESNDSKAQENVKKDPSKAADVFSLPHDQLGQ
                     LVESGVIQEIPEQYSKEIAKNDTKQSLTGAQYKGKTYAFPFGIESQVLYYNKTKLTAD
                     DVKSYETITSKGKFGQQLKAANSYVTGPLFLSVGDTLFGKSGEDAKGTNWGNEAGVSV
                     LKWIADQKKNDGFVNLTAENTMSKFGDGSVHAFESGPWDYDAAKKAVGEDKIGVAVYP
                     TMKIGDKEVQQKAFLGVKLYAVNQAPAGSNTKRISASYKLAAYLTNAESQKIQFEKRH
                     IVPANSSIQSSDSVQKDELAKAVIEMGSSDKYTTVMPKLSQMSTFWTESAAILSDTYS
                     GKIKSSDYLKRLKQFDKDIAKTK"
     gene            1429..2799
                     /locus_tag="ACUBV3_001750"
     CDS             1429..2799
                     /locus_tag="ACUBV3_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HHU3848235.1"
                     /translation="MNKKLISLDGMSLKEVFQKGDLATKLSFLIMGLANLKNKQIVKG
                     LLFLISEILFLITFVYQVIPAVKGLISLGTQEQGMTTKTVDGIKIQVATQGDNSMLML
                     IFGLASLIFCCVFAYIYWSNIKSAAHLLTLKEEGREIPSFKKDIKSLTDGRFHMTLMS
                     IPLIGVLLFTILPLVYMICLAFTNYDHNHLPPKSLFDWVGFANFGNIFSGRMASTFFP
                     VLSWTLIWAVFATVTNFFFGIILALLINTKGLKFKKMWRTIFVITMAVPQFISLLIMR
                     NLLSDAGPVNALLIKWGLISSAHPLPFLSDPVWAKFSIIFVNMWVGIPVTMLVATGII
                     MNLPAEQIEAAEIDGANKFQVFQSITFPQILLIMTPTLIQQFIGNINNFNVIYLLTQG
                     GPTNSTYYQAGSTDLLVTWLYNLTVTAADYNLASVVGILIFILSAVFSLLAYTRTNSY
                     KEGAAK"
     gene            2799..3635
                     /locus_tag="ACUBV3_001751"
     CDS             2799..3635
                     /locus_tag="ACUBV3_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HHU3848236.1"
                     /translation="MNKKKRLNLTFVYILLIVLSIMWLFPIVWVVLTSFRGEGSAFVN
                     YFIPKTWTLDNYAKLFTQNTFPFGQWFLNTLFVATCTCILSTLITVAMAYSLSRIKFK
                     HRNGFLKLALVLNMFPGFMSMIAVYYILKALNLDQTLTALIFVYSAGAALTFYIAKGF
                     FDTIPYSLDESAMIDGATRLDIFLKITLPLSKPIIVYTALIAFMGPWMDFIFAKVILG
                     DATSKYTVAIGLFSMLQQDTINQWFMSFTAGSVIIAIPITILFMFMQKYYVEGITGGS
                     VK"
     gene            3866..5338
                     /locus_tag="ACUBV3_001752"
     CDS             3866..5338
                     /locus_tag="ACUBV3_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HHU3848237.1"
                     /translation="MEKTKSFFGHPRGLSTLFFTEMWERFSYYGMRAILLYYMYYSVS
                     QGGLGMDKTVAASIMAVYGSLVYLSSVIGGFVSDRILGSRKTVLYGGILIMLGHIALA
                     TPFGQTALFISIALIILGTGLLKPNVSEMVGNLYGENDSRRDAGFSIFVFGINLGAFI
                     SPIVVGYLGQEVNFHLGFSLAAIGMFFGLLQYTLDGKKYLTEESLRPNDPLSPEEKSS
                     LYKKVGLILIGIVIVLILLHLMHMLTIEVIIDIFSIIAIAIPIIYFIKILSSKKISSV
                     ERSRVWAYIPLFIASILFWSIEEQGSVVLALFADEQTKLYLNFFGHHINFPSSYFQSM
                     NPLFIMLYVPFFAWLWAKWGSKQPSSPKKFAYGLFFAGASFLWMMLPGLLFGVNAKVS
                     PLWLTMSWAIVIVGEMLISPVGLSATSKLAPKAFQAQMMSIWFLSNAAAQAINAQIVK
                     LYTPDTQILYYGVVGGITVVFGFILLFYVPRIEKLMSGVK"
BASE COUNT         1639 a          987 c          930 g         1808 t
ORIGIN      
        1 tttttaaaaa aagacttgac tttttttgca aacggttgca taatataggt gtaattaaaa
       61 tattctctag gaggagaacc aaaatggaaa aaaatacttg gaaaaaatta cttgttagta
      121 ctgctgctct ttcagtagtt gcaggaggag caattgctgc tactcactct aactcagttg
      181 atgctgcttc aaaaacaact atcaaacttt gggtcccaac agattcaaaa gcgtcttata
      241 aagcaattgt taaaaaattc gagaaggaaa acaaaggcgt tactgtaaaa atgattgagt
      301 ctaatgactc caaagctcaa gaaaacgtaa aaaaagaccc aagcaaggca gccgatgtat
      361 tctcacttcc acatgaccaa cttggtcaat tagtagaatc tggtgttatc caagaaattc
      421 cagagcaata ctcaaaagaa attgctaaaa acgacactaa acaatcactt actggtgcac
      481 aatataaagg gaaaacttat gcattcccat ttggtattga atctcaagtt ctttattata
      541 ataaaacaaa gttaactgct gacgacgtta aatcatacga aacaattaca agcaaaggga
      601 aattcggtca acagcttaaa gcagctaact catatgtaac aggtcctctt ttcctttctg
      661 taggcgacac tttatttggt aaatctggtg aagatgctaa aggcactaac tggggtaatg
      721 aagcaggtgt ttctgtcctt aaatggattg cagatcaaaa gaaaaatgat ggttttgtca
      781 acttgacagc tgaaaataca atgtctaaat ttggcgatgg ttctgttcat gcttttgaaa
      841 gtggaccatg ggattacgac gctgctaaaa aagctgtcgg tgaagataaa atcggtgttg
      901 ctgtttaccc aacaatgaaa atcggtgaca aagaagttca acaaaaagca ttcttgggcg
      961 ttaaacttta tgccgttaac caagcacctg ctggttcaaa cactaaacga atctcagcta
     1021 gctacaaact cgctgcatat ctaactaatg ctgaaagtca aaaaattcaa ttcgaaaaac
     1081 gtcatatcgt tcctgctaac tcatcaattc aatcttctga tagcgtccaa aaagatgaac
     1141 ttgcaaaagc agttatcgaa atgggtagct cagataaata tacaacggtt atgcctaagt
     1201 tgagtcaaat gtcaacattc tggacagaaa gtgctgctat tcttagcgat acttacagtg
     1261 gtaaaatcaa atctagcgat taccttaaac gtctaaaaca attcgataaa gacatcgcta
     1321 aaacaaaata gtacgtctag agtaatgaga tgaggtgagt actcttcctt tcaattgaga
     1381 gtgcttccca tctcttattt tgtaaaataa caattaaagg agaccattat gaataaaaaa
     1441 ctgatctcac ttgatggtat gtccctaaaa gaggtctttc aaaaaggtga tttagcaact
     1501 aaactatcat tcttaattat gggacttgct aacctcaaaa ataaacaaat tgtcaaggga
     1561 cttttatttt taatcagtga aattctgttt ttaattacct ttgtttacca agttatccca
     1621 gctgtaaaag gtcttatttc cttagggacc caagaacaag gtatgacaac taaaactgtg
     1681 gatggtatta aaatccaagt tgcgacacaa ggtgataact ctatgttaat gctaattttt
     1741 ggattagctt cgcttatctt ctgttgcgta tttgcctata tttactggtc aaacattaaa
     1801 agcgcagcac atttattgac cttgaaagaa gaaggaagag aaattccatc cttcaaaaaa
     1861 gatattaaaa gcttgacaga tggacgcttc catatgactt taatgtctat tcctttaata
     1921 ggggtgcttc tatttacaat ccttccgtta gtttatatga tttgtttggc atttactaac
     1981 tatgatcata accacttacc gccaaaaagt ttatttgact gggttggttt tgctaacttc
     2041 ggtaatatct ttagcgggcg tatggccagt actttcttcc ctgttctatc ttggacatta
     2101 atttgggctg ttttcgctac tgtaacgaat ttcttctttg gtattattct tgccttacta
     2161 attaatacaa aaggtttgaa attcaaaaag atgtggcgta ctatttttgt tattaccatg
     2221 gctgtccctc aatttatctc tctcttaatc atgagaaacc tcttaagtga cgctggaccg
     2281 gttaacgcct tgttaataaa atggggactt atttcttcag cacatcccct tccattcctt
     2341 tctgatccgg tctgggcgaa attctcaatc atctttgtta atatgtgggt tggtattcct
     2401 gtaacaatgc ttgttgctac tggtatcatc atgaatttac ctgcagaaca aattgaagct
     2461 gctgaaattg atggggcgaa taagttccaa gtctttcaat caattacttt cccacaaatt
     2521 ttgttgatta tgactcctac tttaattcaa caatttatcg gaaatattaa caacttcaac
     2581 gttatctacc tactcactca aggtggacca acaaactcaa catactacca agcaggtagc
     2641 accgatttac ttgtcacttg gctatacaat ttgacagtaa ctgctgcaga ttataactta
     2701 gcatctgttg ttggtatctt aatctttatt ctttcagcag tatttagttt actagcttac
     2761 acaagaacaa attcttacaa ggaaggggca gctaaataat gaataagaaa aaacgtctta
     2821 accttacttt tgtttatatt ttattgatag tactatcaat tatgtggctc tttcccattg
     2881 tttgggtcgt tctcacaagc ttccgtggtg aaggaagtgc ctttgtcaac tactttattc
     2941 ctaaaacttg gacattagac aactacgcta aactcttcac ccaaaacaca ttcccatttg
     3001 gtcaatggtt cttaaataca ttatttgtcg ctacatgtac ttgtatacta tcaactttaa
     3061 tcactgtagc aatggcttat tctctcagca gaatcaaatt caaacaccgt aatggtttcc
     3121 taaagctagc tttagtcttg aatatgtttc ctgggttcat gtcaatgatt gcagtttact
     3181 acatcctaaa agctctaaat ctagaccaaa cattaaccgc tttaatattt gtctattctg
     3241 caggagcagc attaaccttc tacatcgcaa aaggtttctt tgatactatt ccatactctt
     3301 tggatgagtc tgctatgatt gatggggcga cccgtttgga tattttcctt aaaatcaccc
     3361 taccattgtc aaaacccatt atcgtttata ctgcattaat cgcttttatg ggcccttgga
     3421 tggactttat ctttgcaaaa gttattttag gtgatgctac gagcaagtac acagtcgcta
     3481 ttggtctctt ttcaatgttg caacaagata ccatcaacca gtggttcatg tcatttacag
     3541 caggatctgt tattattgca attccaatca caatactttt catgttcatg caaaaatact
     3601 atgttgaagg tattacaggt ggatctgtaa aataatcttc agataattta aagacacgac
     3661 atttttattt cactgtggtt tcttttctaa aaaaggcttg ggttctattg aattcaagcc
     3721 tttttctaag ctagatagtt aaaaaacttg gtttaccaag ggtgttaacc tctacaaaaa
     3781 aataacttga tagtgctaag taatttgaca gttgcttcta tttactatat tattaatagt
     3841 gttatctaca aaaggagcgt ttttcatgga aaaaacaaaa tcattctttg gacatcctcg
     3901 tggtttgtcc actcttttct ttactgaaat gtgggaaaga ttctcatact atggtatgcg
     3961 tgctattttg ctgtactata tgtactatag tgtttctcaa ggtggccttg gtatggacaa
     4021 gactgtcgct gcatcaatca tggctgtcta tggttcactg gtttatctct catcagtaat
     4081 tggtggtttt gtcagcgacc gtattctagg tagtcgtaaa actgttctgt atggtggtat
     4141 tctaatcatg ctaggtcata ttgctctagc tacaccattt ggtcaaacag ctctattcat
     4201 ttcgattgct ctaattatcc ttggtactgg actattaaaa ccaaatgtat cagagatggt
     4261 tggtaattta tatggagaaa atgattctcg tcgtgatgct ggttttagta tctttgtttt
     4321 tggtattaac cttggtgctt ttatttcacc cattgtagtg gggtacctcg gacaagaagt
     4381 aaatttccat cttggtttct cacttgctgc tattggtatg ttctttggtc tcctccaata
     4441 taccttagat ggaaaaaaat atttgactga agagagtctc agaccaaacg atcctttaag
     4501 tcctgaagaa aagtcctctc tatataaaaa agttgggctt atccttattg gtattgttat
     4561 tgtacttatt ctacttcact tgatgcatat gctaacaatt gaagtaatta tcgatatttt
     4621 tagtattatt gcaatcgcca tcccaattat ttattttatc aagattttaa gtagtaaaaa
     4681 gatttcttct gttgagcgtt ctcgagtgtg ggcatatatc cctctcttta tcgcctcaat
     4741 tctattttgg tcaattgaag aacaaggttc agttgtctta gccttatttg cagatgaaca
     4801 aacaaaactt taccttaact tctttgggca tcatattaat ttcccatcaa gttatttcca
     4861 aagtatgaac cctctcttca ttatgcttta tgtaccattc tttgcttggt tatgggctaa
     4921 atggggaagt aagcaacctt catcacctaa aaaatttgcg tatggacttt tctttgctgg
     4981 agcttcattc ttatggatga tgctaccagg tttactcttt ggagttaacg ctaaagtaag
     5041 ccctctttgg ttaacaatga gttgggctat tgtcatcgtt ggggaaatgc taatctcacc
     5101 agttggatta tcagcaacta gtaagctcgc acctaaagca ttccaagctc aaatgatgag
     5161 catctggttc ttaagtaatg ctgcagcaca agctattaac gctcaaatcg ttaaattgta
     5221 cacacctgat actcaaattc tttattatgg tgttgttggt ggtataacag ttgtatttgg
     5281 atttatcctc ttattttatg ttccacgcat tgaaaaacta atgtctggag ttaaataaag
     5341 acatctaaaa agaactaggt taca
//
LOCUS       DBJYSH010000116         5328 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.116, whole genome shotgun sequence.
ACCESSION   DBJYSH010000116 DBJYSH010000000
VERSION     DBJYSH010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 5328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5328
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.116"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            152..541
                     /locus_tag="ACUBV3_001753"
     CDS             152..541
                     /locus_tag="ACUBV3_001753"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HHU3848238.1"
                     /translation="MGNIELKKLAVKASENAYVPYSKFPVGAALRTAEGKIFTGCNVE
                     NISYGLANCAERTAIFKAVSEGYKDFSEIAIYGNTEEPISPCGACRQVMVEFFNKNAK
                     VTLIAKNGKTVETTVGELLPYSFVDLK"
     gene            606..1655
                     /locus_tag="ACUBV3_001754"
     CDS             606..1655
                     /locus_tag="ACUBV3_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019315854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family lipoprotein"
                     /protein_id="HHU3848239.1"
                     /translation="MNKKIAGIGLASIAVLSLAACGHRGASKSGGKSDSLKVAMVTDT
                     GGVDDKSFNQSGWEGMQAWGKKNGLKKGAGFDYFQSASESDYATNLDTAVSSGYKLIF
                     GIGFSLHDAIDKAADNNKDVNYVIVDDVIKGKDNVASVVFADNESAYLAGIAAAKTTK
                     TKTVGFVGGMESEVITRFEKGFEAGVKSVDKSIKIKVDYAGSFGDAAKGKTIAAAQYA
                     SGADIVYQVAGGTGAGVFSEAKSRNESLKEADKVWVLGVDRDQAAEGKYTSKDGKASN
                     FVLASSIKEVGKSVELIATKTSKGKFPGGNVTTYGLKDGGVDIATTNLSDDAVKAIKE
                     AKAKIISGDIKVPSK"
     gene            1800..3335
                     /locus_tag="ACUBV3_001755"
     CDS             1800..3335
                     /locus_tag="ACUBV3_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HHU3848240.1"
                     /translation="MTQNVIEMKEITKKFGDFVANDHINLTVEKGEIHALLGENGAGK
                     STLMNMLAGLLEPTDGQIFINGQPVTIDSPSKSSQLGIGMVHQHFMLVEAFTVAENIV
                     LGNETTQNGVLDIKTAAKEIKELSEKYGLSVNPNAKISDISVGAQQRVEILKTLYRGA
                     DILIFDEPTAVLTPSEIKELMTIMKSLVKEGKSIILITHKLDEIRAVADKVTVIRRGK
                     SIETVPVAGASSQQLAEMMVGRSVSFRTEKKEANPTDIILSVKDLVVEENRGGVLAVK
                     NLSLDVRAGEIVGIAGIDGNGQSELIQAITGLRKVTSGQIVIKGKDVTKFSSRQITEL
                     SVGHVPEDRHRDGLVLDMTMAENLALQTYYKEPLSHKGILNFAKIKEYARQLMTEFDV
                     RGAGEHVLARGFSGGNQQKAIIAREVDRDPDLLIVSQPTRGLDVGAIEYIHKRLIEER
                     DKGKAVLVVSFELDEILNLSDRIAVIHDGKIQGIVKPDQTNKQELGILMAGGKIEKEE
                     RDV"
     gene            3328..4389
                     /locus_tag="ACUBV3_001756"
     CDS             3328..4389
                     /locus_tag="ACUBV3_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HHU3848241.1"
                     /translation="MSKKAQKIAVPLISVVLGIILGAIIMLIFGYDPLWGYEGLFQTA
                     FGSIKNIGEIFRAMGPLILIALGFSVASRAGFFNIGLPGQALSGWIAAGWFALSHPDM
                     PRPAMILCTIIIGIVAGGITGAIPGILRAYLGTSEVIVTIMMNYIVLYSGNAIVQRVF
                     PKSIMRTSDSSVYVSANASYQTDWLSSLTNNSRINIGIFIAIIAVVLVWFLLNKTTLG
                     FEIRSVGLNPNASEYAGMSAKRTIILSMIISGAFAGLGGVVEGLGTFENVFVQPSSLA
                     IGFDGMAVSLLAANSPIGILFAAFLFGVLSVGAPGMNIAGIPPELIKVVTASIIFFVG
                     VHYIIEYVIKPKKQMKGGK"
     gene            4391..>5328
                     /locus_tag="ACUBV3_001757"
     CDS             4391..>5328
                     /locus_tag="ACUBV3_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HHU3848242.1"
                     /translation="MSLTTIFALLFSSMLIYATPLIFTSIGGTFSERGGIVNVGLEGI
                     MVIGAFSGVVFNLEFASVFGDATPWISVLVGGLVGLIFSVIHAVATVNFRADHIISGT
                     VLNLMAPSLAVFLIKVLYNKGQTDNIQESFGKFNFPILSDIPFVGDIFFKGTSLVGYI
                     AILFSFLAWFILYKTRFGLRLRSVGEHPQAADTLGINVYLMRYSGVLISGFLGGIGGA
                     VYAQSISVNFAATTILGPGFISLAAMIFGKWNPIGAMLASLFFGLSQSLAVIGSHLPL
                     LSNIPTVYLQIAPYVLTIIVLAAFFGQAVAPKADGI"
BASE COUNT         1638 a          804 c         1152 g         1734 t
ORIGIN      
        1 aaaatagatt cagctaaaat accaagacta atgaaactac aagacttggt tactcaataa
       61 taaatacttc tgtgtttgat aagaagataa taaacgtgtt ctaaaaattt tgccagtaaa
      121 cattatgaag cgaaaggtgc agctactaaa aatgggaaat atagagttga aaaaattggc
      181 tgtaaaagcg agtgaaaatg catacgttcc ttactcgaaa tttccagtag gcgcagcatt
      241 gcgtacagca gaaggaaaaa tatttacagg atgtaacgtt gaaaatatca gttatggatt
      301 agcaaactgt gctgagcgta ctgcaatttt taaagctgtt tcagaaggtt ataaagattt
      361 ttcggaaatt gctatttatg gaaatactga agaaccaatt tctccttgcg gtgcatgccg
      421 tcaagtgatg gtggaatttt ttaataagaa tgctaaagtt acattgattg ctaaaaatgg
      481 taagacggtc gagacgacgg tcggtgagtt gttaccatac tcgtttgttg atttaaaata
      541 atttaaatca gttgctcttt taagcattta gcttaaatat taagtttttt aggagggttc
      601 atttcatgaa caaaaaaatt gccgggatcg gcttggcttc gattgcagta cttagtttag
      661 ctgcatgtgg acatcgtggt gcttctaaat ctggtggtaa atcagatagc ttgaaggttg
      721 caatggtaac agataccggt ggtgttgatg ataaatcatt taaccaatct ggttgggaag
      781 gtatgcaagc ttggggcaag aagaatggcc ttaaaaaagg agctggtttt gactatttcc
      841 aatcggcaag tgaatctgat tatgcaacta acttagatac agctgtgtct agtggttata
      901 aattgatttt cggtattgga ttttctcttc atgatgctat tgataaagca gcagacaata
      961 acaaagatgt taattacgtc atcgttgatg atgttattaa agggaaagat aatgttgcaa
     1021 gtgttgtctt tgcggataat gaatcagctt acttagcagg tattgcagcc gctaaaacta
     1081 ccaaaacaaa aacagttggc tttgtaggtg gtatggaatc tgaggttatt acccgttttg
     1141 aaaaaggttt tgaagcaggt gtcaaatcag ttgataaatc aattaaaatt aaagttgact
     1201 atgctggttc attcggtgat gctgctaagg gtaagacaat tgcagccgca caatatgctt
     1261 ctggcgcaga tattgtttat caagtagctg gtggaactgg tgcaggtgta ttcagtgaag
     1321 caaaatcacg caatgaatct ttaaaagaag ctgacaaagt ttgggtatta ggtgtagacc
     1381 gtgaccaagc agcagaaggt aagtatactt ctaaagatgg taaggcttct aactttgttt
     1441 tggcttcatc aattaaagaa gttggaaaat cagttgaatt gattgctact aaaacaagta
     1501 aaggtaaatt cccaggtggt aatgtaacaa cttatggttt gaaggatggt ggagttgata
     1561 tagcaactac aaacctttca gatgacgccg ttaaagcgat taaagaggct aaagctaaaa
     1621 ttatttcagg agatatcaaa gttcctagca aataatatct ttaagaactt tcaaaaaagc
     1681 gattagaaat cgcttttttg aagctgagat aaacttatat ctcagtaaaa tccatcagtc
     1741 gttaacgatt ggtggatttt actgaactat aaatgaattt ttctgaaagg gagaaccaca
     1801 tgacacagaa tgtcattgaa atgaaagaaa tcactaagaa attcggtgat tttgtggcaa
     1861 atgatcatat caatttgacg gttgaaaaag gtgagataca tgctctgctt ggcgaaaacg
     1921 gtgcagggaa atcaacttta atgaacatgt tagcaggttt actagaacca acagacggtc
     1981 aaatttttat taatggtcag cctgtaacta ttgattcacc ttccaaatcg tcacaattgg
     2041 ggattggtat ggtacatcag catttcatgc ttgttgaagc atttactgta gctgaaaata
     2101 ttgttctagg aaatgaaacc acccaaaatg gtgttttaga cattaagaca gcagctaaag
     2161 aaataaagga actatcagag aaatatggtt tgtcagtaaa tccaaatgct aaaatatctg
     2221 atatttctgt aggagcacag cagcgtgttg aaattttgaa gacactttat cgcggtgcag
     2281 atattttaat ttttgatgag ccgacggcgg tgcttacacc atcagaaata aaagaattga
     2341 tgacaattat gaagagcctt gttaaagaag gcaaatccat tatactaatt acccataaat
     2401 tggatgaaat tcgggcagtt gctgataaag ttactgtgat tcgccgtggt aaaagtattg
     2461 aaacagtccc tgttgcaggc gcaagttctc aacaactggc ggaaatgatg gttggacgat
     2521 ctgtttcctt cagaactgaa aaaaaggaag ctaatccgac tgacataatc ctttctgtaa
     2581 aggatttagt tgtagaagaa aatagaggcg gcgttctagc tgttaaaaat ctgtcgttag
     2641 atgttcgagc aggtgaaatt gtaggtatag ctggtattga tggtaatgga caaagtgaat
     2701 taattcaagc tattacaggt ttaagaaagg taacttctgg tcaaattgta attaaaggta
     2761 aagatgtcac aaaattttca agtcgacaaa ttacagaatt aagtgtaggt catgttccag
     2821 aagatcgtca tagagatggt ttggttctag atatgacgat ggcagaaaat ttagctttac
     2881 aaacttatta taaagagcca ttgagtcata aaggtatcct taattttgct aaaattaaag
     2941 agtatgctcg tcaattaatg acagaatttg atgtgcgtgg tgctggtgaa catgttttag
     3001 caagaggatt ttctggtggt aaccaacaaa aagctattat tgctcgtgaa gtggatcgcg
     3061 atccagacct actcattgtt agccaaccga ctcgtggtct agatgttggt gctatcgagt
     3121 atattcataa acgtttgatt gaagagcgcg ataaagggaa agcggtctta gtagttagct
     3181 ttgagttaga tgaaatctta aacttatcag atcgtattgc agttattcat gatggtaaaa
     3241 ttcaaggaat tgttaaaccc gaccaaacca acaagcaaga attgggtatt ttgatggctg
     3301 gtggaaaaat tgaaaaggag gagagagatg tctaagaagg cgcaaaaaat tgcagttcct
     3361 ctgatttcag ttgtactagg aatcattcta ggagctatta ttatgctgat atttggttat
     3421 gatccacttt ggggttatga gggattgttc caaacagcat tcggtagtat aaaaaatatt
     3481 ggtgaaattt tccgtgcaat gggaccatta attttaattg ctctaggttt ctcagttgcc
     3541 agtcgtgcag gtttctttaa tattggactt ccagggcaag ccttatcagg atggattgcc
     3601 gcaggttggt tcgctctttc acacccagat atgccacgtc cagcaatgat tctttgtact
     3661 attattattg gtatcgttgc tgggggaatc actggagcta ttccaggtat tctcagagca
     3721 taccttggaa caagtgaagt tattgtaact attatgatga actatattgt tctttattca
     3781 ggaaatgcta ttgtacaacg tgttttccca aaaagcatta tgcgtacaag tgactcgagt
     3841 gtatatgttt ctgccaatgc atcataccag acagattggt tatctagttt aacgaataat
     3901 tcacgtatca atattggtat cttcattgct attattgcag ttgtgttagt ttggttccta
     3961 ttaaataaaa caacgcttgg atttgaaatt cgttcagttg gtttaaatcc aaatgctagt
     4021 gagtacgctg gtatgtctgc aaagcgtaca attattctat caatgattat ttctggagct
     4081 tttgcaggac ttggtggtgt tgttgaagga ctaggtacat ttgagaatgt atttgtacag
     4141 ccatcctcgt tagctattgg ctttgacggt atggcagtaa gtctactcgc agcgaatagt
     4201 ccaataggta ttttatttgc agcatttcta tttggtgttc taagtgttgg tgcgcctggt
     4261 atgaacattg caggtattcc acctgagtta attaaagttg taacagcttc aatcatcttc
     4321 ttcgtaggtg tccattacat tattgagtat gttattaaac ctaaaaaaca aatgaaaggt
     4381 ggtaagtaaa ttgagtttaa caacgatttt tgcattgcta ttttcatcaa tgctaattta
     4441 cgcaacacct cttatcttta caagtattgg gggaaccttc tctgaacgtg gtggtatcgt
     4501 caacgttggt ttagaaggaa ttatggtaat tggagctttc tcaggcgttg tatttaattt
     4561 agagtttgct tctgtttttg gtgatgcgac tccatggatt tctgtactag ttggtggttt
     4621 agttggttta attttttctg ttatccatgc agttgcgaca gttaattttc gtgcagatca
     4681 tattatcagc ggaaccgttc ttaatttaat ggcaccttca ttagctgttt tcttgattaa
     4741 ggttctttat aataaaggtc aaactgataa tattcaagaa tcatttggaa aatttaattt
     4801 ccctatacta tcagatattc cttttgttgg tgatatcttc tttaaaggaa caagcttagt
     4861 cggttatatt gctattcttt tctcattcct tgcttggttt attctatata aaactcgctt
     4921 tggattaaga ttacgctcag ttggagaaca tcctcaagca gcagacacct taggtattaa
     4981 tgtttatctt atgcgttact ctggtgtctt gatttcaggt ttccttggtg gaataggagg
     5041 agctgtatat gctcaatcta tttctgttaa ctttgcagcg acaactatat taggaccggg
     5101 ctttatctcc ttagcggcga tgatttttgg taagtggaat ccaattggtg caatgcttgc
     5161 tagtctattc tttggtttat cgcaaagttt agcagttatc ggtagccatt taccactttt
     5221 gagcaatatt ccaacagttt atctacaaat tgctccatat gtcctcacta tcattgtatt
     5281 agcagctttc tttggacaag cagtagctcc taaggcggat ggtattaa
//
LOCUS       DBJYSH010000117         5319 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.117, whole genome shotgun sequence.
ACCESSION   DBJYSH010000117 DBJYSH010000000
VERSION     DBJYSH010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 5319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5319
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.117"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            complement(2..1021)
                     /locus_tag="ACUBV3_001758"
     CDS             complement(2..1021)
                     /locus_tag="ACUBV3_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001932930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HHU3848243.1"
                     /translation="MFLLLCILSMWLMIFGVAFYYFGSLHQSLTSRIIYQFVLTVLLT
                     TTAWFMGAYFLAFEGHFKTVFQFQEADGKQIVNCLFQLCFALYAVVMLIGSIIDRVQT
                     KRLLLAVVSWLFLVYTPLAYLIWNSEGVFAKMGVLDFSGGMVVHLSAGLSSYILAHVI
                     GKSEHQHNKVKNDSLFLGMILITFGWFGFNMGPVGEWNSQAIMILLNTIFAIIGGGLA
                     WTLAAKWNGEEEKTGSLLNGIIVGLVTSTAGVGYLLTWQLLAVTFFASLFTYFVTDYV
                     AKAFAIDDVVSSFGMNGIGGLLGSLGVGLFKLSHMPVQLLALATTILLSIIMTYIISK
                     AIFRK"
     gene            1125..2252
                     /locus_tag="ACUBV3_001759"
     CDS             1125..2252
                     /locus_tag="ACUBV3_001759"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HHU3848244.1"
                     /translation="MKHYQTIIIGAGAAGIGFGSAMQRLGLTNFLIIEKGHIGESFLR
                     WPRTTQFITPSFTTNGFGFPDLNAVIPDTSPAFSFEKEHLSGVEYARYLQLVAAHYNL
                     PIQNETSVLSIDKRDSLFVIKTSKGDFSADYLIMATGEFQNPNTIDIKGADLGMHYGQ
                     VDNFHIKSDNPFIIIGGNESACDALTHLVYLGNQVELYTDTFGRKESNPDPSISLSPL
                     TKERLKHIQDHKKEYYSISEGKKAIEIKQIGKQYQVTFDDGSTAESFHKPILSTGFLN
                     TCHLIDGIALFEYDKNQLPIVTEDDESTIVNNCFLIGPSLRQRDTIFCYIYKFRQRFV
                     PVINHIAQREGIILDEDTLEFFKENQMYLDNLACCDVNCDC"
     gene            complement(2289..3275)
                     /locus_tag="ACUBV3_001760"
     CDS             complement(2289..3275)
                     /locus_tag="ACUBV3_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HHU3848245.1"
                     /translation="MSEKIRVLLYYKYVSIENAEEYAAKHLEFCKSIGLKGRILIADE
                     GINGTVSGDYETTQKYMDWIHSDERFADLWFKIDEENQQAFRKMFVRYKKEIVHLGLE
                     DNNFDSDINPLETTGEYLNPKQFKEALLDEDTVVLDTRNDYEYDLGHFRGAIRPDIRN
                     FRELPQWVRDNKDKFMEKRVVVYCTGGVRCEKFSGWMVREGFKDVGQLHGGIATYGKD
                     PEVQGELWDGAMYVFDDRISVPINHVNPTVISKDYFDGTPCERYVNCANPFCNKQIFA
                     SEENEAKYVRGCSPECRAHERNRYVQENGLSRQEWAERLEAIGESLPQVANI"
     gene            3438..3744
                     /locus_tag="ACUBV3_001761"
                     /pseudo
     CDS             3438..3744
                     /locus_tag="ACUBV3_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220570.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3902..5275)
                     /locus_tag="ACUBV3_001762"
     CDS             complement(3902..5275)
                     /locus_tag="ACUBV3_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000914222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HHU3848246.1"
                     /translation="MLKKLFAPPQKRERLSAEEIKHKYPRYRVQVLISIFVGYMGYYF
                     VRNTTSILSGILNMSATEIGIITCASYIAYGLSKFISGLISDESNSKIFLPVGLFLTG
                     LVNVLIGVIPSVITSIWLFTIMYLINGWLQGMGYPPGARTLVYWYDNKERIKYATIWN
                     LSHNFGGAIAPILTGVGLALAGNDSLNQARAAYWFPGVVACLLAVLVYFLQEDTPESI
                     GLPPIEEYHKEQYTNVVDSSDILEEPEVLGMGEIIKKYILPNTKLMWASLYSIFVYIL
                     RYGIVSWTPKFLATSVQDGGKGITATAGMGGFSLFEIGGIIGMLTAGYLSAKVFKNSK
                     PLTNVAFLVVAILLLAAYWFIPAGPQYMALDFIILLGLGASIYGPVMMVGLYAMELVP
                     KAAAGAASGLTGTFSYVGGATIATLAIGIIIDHFGWGVAFIIFGISGFAAIVCTLLSR
                     DKSLEYW"
BASE COUNT         1871 a         1089 c          784 g         1575 t
ORIGIN      
        1 attattttct aaaaatcgct ttagatatga tataggtcat tataattgac aaaagaattg
       61 tggtagctaa tgctaacagt tgaactggca tatgtgataa tttaaagaga ccaactccta
      121 aacttcccaa caatccccca ataccattca ttccaaagga ggaaacgaca tcatcaatgg
      181 caaaagcttt cgcaacgtaa tccgttacaa agtaggtgaa gagggatgca aagaatgtta
      241 cagctaataa ttgccaagtt aagagatacc caactcctgc tgtactcgtc acaagaccaa
      301 caataatgcc attcaataag cttcctgttt tctcttcctc accattccac ttagcagcca
      361 atgtccacgc caaaccacca cctataatag caaatatagt atttaaaagt atcataatgg
      421 cttgtgaatt ccactcacca actggaccca tattaaatcc aaaccaacca aatgtaataa
      481 gtatcattcc taaaaagaga ctatcatttt taaccttatt gtgctgatgt tcactcttgc
      541 caataacatg agctaaaata tacgaagaaa gccctgcaga taaatgaacg accattccgc
      601 ctgaaaaatc aagtactccc atctttgcaa aaacaccctc tgaattccaa attaaataag
      661 ctaagggcgt atacaccaag aaaagccaag aaacaacagc taataaaagt cgcttcgtct
      721 gaactctatc aataattgac ccaataagca ttactactgc gtaaagggcg aaacaaagtt
      781 gaaataaaca atttacaatt tgtttaccat ctgcttcttg gaattgaaag acagttttaa
      841 aatgaccttc aaatgctaaa aaataggcac ccatgaacca agctgttgtc gttagcaaaa
      901 cagtaagtac aaactgataa ataatccttg aagtcaatga ttgatgtagc gacccaaagt
      961 aatagaatgc aaccccaaaa atcatgagcc acatcgataa aatacataat agtaaaaaca
     1021 caaatagtcc tttctttaca aacactcttt tatttgttaa actgtattgt tgttaactgg
     1081 ttaagtattt tatcttacac atttattttt gtcaaggaga agacatgaaa cattatcaaa
     1141 ccataattat cggtgcaggc gctgcaggaa tcggttttgg ttctgccatg caacgattag
     1201 gacttacaaa ttttcttatc attgaaaaag ggcacattgg agaatctttt ttaagatggc
     1261 ccagaacaac acaatttatc acaccttcct tcactaccaa tggctttggt tttccagatc
     1321 taaacgctgt tattccagat acttctcctg ctttctcatt tgaaaaagaa catctttctg
     1381 gagtggaata tgcacgctac ttacaattag ttgctgctca ttataatctc cctatccaaa
     1441 acgaaacatc tgttctatct attgataaac gagatagcct ttttgttatt aaaacttcaa
     1501 agggagattt ttctgctgat tatcttatta tggcaacagg agaatttcaa aatccaaata
     1561 ccattgatat taaaggtgca gatctaggaa tgcactatgg tcaagttgat aatttccata
     1621 tcaaatctga caatccattt atcatcattg gtggaaacga aagtgcttgt gatgcactaa
     1681 cacatctagt atatttggga aatcaagtcg aattatatac agatacgttt ggtcgtaaag
     1741 agagcaatcc cgatccaagc atctctttat cccccctaac taaagaacgt ttaaaacata
     1801 tacaagacca caaaaaagaa tattatagta tctctgaagg caagaaagcc attgagatta
     1861 aacaaattgg aaaacaatat caagttactt ttgatgacgg tagtactgcg gagtctttcc
     1921 ataaacctat tctttccaca ggttttctaa atacctgtca tctcattgac ggaatcgctt
     1981 tatttgaata cgataaaaat cagttgccaa tagttactga agatgacgaa tctactatcg
     2041 ttaataactg tttcttaatt ggtccaagct tacgacaaag agatactatt ttctgctata
     2101 tttataaatt cagacagcgc tttgtccctg ttattaatca tatcgcacaa cgagaaggga
     2161 ttatattaga cgaagatacc cttgaattct ttaaagaaaa tcaaatgtac cttgataacc
     2221 tagcttgttg cgatgtcaat tgtgattgct aaaaaatcgc tagctttatt gctagcgatt
     2281 ttttaatatt aaatatttgc tacttgaggt aaggattctc caatagcttc taaacgttct
     2341 gcccattctt gacgtgataa accattttct tgcacatagc gattacgttc atgtgcacga
     2401 cattcaggcg agcaccctcg aacatacttc gcttcgtttt cttctgaagc aaaaatttgt
     2461 ttgttacaaa acggatttgc acaattgaca taacgttcac aaggtgttcc atcaaaataa
     2521 tctttactaa taacagttgg attaacatga ttaattggca ccgagatacg atcatcaaaa
     2581 acatacatcg caccatccca aagttcacct tgaacttccg gatcttttcc atatgttgca
     2641 ataccaccgt gtaattgtcc aacatcttta aaaccttctc gaaccatcca acccgagaat
     2701 ttctcacaac gaacaccacc ggtacagtac acaacaacac gtttttccat aaacttatct
     2761 ttgttgtcac gtacccattg tggtaactca cggaaattgc gaatatcagg acgaattgct
     2821 ccacggaagt gacctaagtc atactcataa tcattacgcg tatctaaaac aacagtatct
     2881 tcatcaagca aggcttcttt aaattgttta ggatttaaat actcacctgt tgtttccaaa
     2941 ggattaatat cgctatcaaa gttattatct tctaacccaa ggtgcacaat ctctttctta
     3001 taacgaacaa acattttacg gaaagcttgt tgattttcct cgtcaatctt gaaccaaaga
     3061 tcagcaaaac gctcatcgct gtggatccaa tccatatact tctgagttgt ttcataatct
     3121 ccagaaacag ttccattaat cccctcatcc gcaatcaaaa tacgaccttt aagcccaata
     3181 gatttacaaa attctagatg tttcgcagcg tactcttccg cattttcaat tgaaacatat
     3241 ttataataga gtaaaactct gattttttct gacatttttt ctcctcatct gcaaaataag
     3301 ttagtatact tcattatata acgactcctt acaacatagc aaaaaatacg cttaggacat
     3361 attctttgct ttaaagacaa attattcttc ttaagaacac acatatgtta aaatagtaaa
     3421 agactaggag gacttttatg acttatgaat tatgtctaga atacggtact tatccactta
     3481 gacctgtaga tgcttgggct gatgaaatca atactgcacc tgcgtttatt actgaagata
     3541 aaaaaacttt tagaactttt agaagaagtc aataccttat tccatgagct ctttttaacg
     3601 atagaatgtt ccttccacta tattggtcat gacttcccag aaaaaagagc aaaaatcaca
     3661 caaatttatc atgtgattat cgaacattta agcatacact accctgaata tgacattaaa
     3721 attgaatcat tattaatggg gtagttttat tttaactagg tagaaactat ctattaaaaa
     3781 caatcgatat cactaattaa ttcaacaaat agattatcta aaacaaaaaa ggtctctttc
     3841 aagaggcctt ttttcataac ttatctcact aaatattaaa tgatactttt accaaataat
     3901 atcaccaata ctctagactc ttatctcttg aaagcaaggt acagacaata gccgcaaatc
     3961 cagaaatacc aaatattata aatgctactc cccaaccaaa gtggtcaata ataataccaa
     4021 tagctagagt agcgatcgtt gctcctccga cataactaaa tgtgccagtc aatcctgaag
     4081 ctgctccagc tgctgctttt ggaactaact ccatagcata caggccaacc atcataacag
     4141 gaccgtagat tgatgccccc aaacctagta aaataatgaa gtctaatgcc atatattgag
     4201 gtcctgctgg aataaaccaa taggcagcta gtaataagat tgctacaact aagaaggcta
     4261 cattggttaa aggtttagag tttttaaaca ctttggcaga tagatatcct gctgttagca
     4321 tcccaataat accacctatt tcaaaaagtg aaaaaccacc cataccagct gttgcagtaa
     4381 taccttttcc accatcttgc acactcgtcg ctaaaaactt aggagtccat gagacaatac
     4441 cgtaacgtaa aatatataca aatattgaat acaaggatgc ccacatcagt ttagtatttg
     4501 gtagaatata tttttttata atttcgccca tacccaaaac ttcaggctct tctaaaatgt
     4561 cacttgaatc cacaacatta gtatattgct ctttatgata ctcttctata ggcggaagtc
     4621 cgatagattc tggagtatct tcttgtaaaa agtaaacaag aacagccaaa agacaagcca
     4681 ccacccctgg aaaccaataa gcagcacgcg cttgattaag agaatcatta ccagcaagag
     4741 ctagacctac accagttaaa atcggcgcta ttgctccacc aaaattgtga gataaattcc
     4801 aaatggtagc atatttaatt ctttctttat tatcatacca atagactaat gtacgcgctc
     4861 caggagggta tcccatccct tgtaaccaac cattaataag atacatgata gtaaagagcc
     4921 agatactagt aattacagat ggaataacac cgattaacac attaactagt cctgtaagaa
     4981 acaaaccaac tggaaggaaa attttagaat tcgattcatc tgaaatcaaa ccagaaataa
     5041 atttagagag accataagca atgtagctag cacaggtaat aataccaatc tcagtagcag
     5101 acatattaag gataccagat aaaattgaag tcgtattacg aacaaaatag tatcccatat
     5161 aacctacaaa aatagaaata aggacttgta cacgataacg aggatattta tgcttaattt
     5221 cctcagctga taaacgttct cttttctgag ggggtgcaaa tagttttttc aacatagcaa
     5281 attacctttc cgatttttag atgtgtttaa ttatacaac
//
LOCUS       DBJYSH010000118         5242 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.118, whole genome shotgun sequence.
ACCESSION   DBJYSH010000118 DBJYSH010000000
VERSION     DBJYSH010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 5242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5242
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.118"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            200..619
                     /locus_tag="ACUBV3_001763"
     CDS             200..619
                     /locus_tag="ACUBV3_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HHU3848247.1"
                     /translation="MDNCIFCKIISGEISSSKVYEDDEVLAFLDITQTTTGHTLLIPK
                     KHVRNVLEMDEKTAQITFERLPKVARAVQAATKAKGMNIINNNEEIAGQTVFHAHVHL
                     VPRFDESDGIKIHYTTHEPDFEALAKLAKEIRKEIQL"
     gene            616..912
                     /locus_tag="ACUBV3_001764"
     CDS             616..912
                     /locus_tag="ACUBV3_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3848248.1"
                     /translation="MKIGRLIALGLVSLGALELYKNRKTIKDSYQNTKNETDSAKLKL
                     ERIKNDLAIISQEKEKIRLISQELNHKFQVFNKDIQPRLEEINQRMAKYQEKDE"
     gene            complement(976..2283)
                     /gene="brpA"
                     /locus_tag="ACUBV3_001765"
     CDS             complement(976..2283)
                     /gene="brpA"
                     /locus_tag="ACUBV3_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000708155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation/cell division transcriptional
                     regulator BrpA"
                     /protein_id="HHU3848249.1"
                     /translation="MKIWKKITLMFSAIILTTVIALGVYVASAYNFSTNELSKTFKDF
                     KLAKSKSHAIEETKPFSILLMGVDTGSEHRKSKWSGNSDSMILVTINPKTNKTTMTSL
                     ERDVLIKLSGPKNNGQTGVEAKLNAAYASGGAEMALMTVQDLLDINVDYFMQINMQGL
                     VDLVNAVGGITVTNKFDFPISIAANEPEYKAVVEPGTHKINGEQALVYSRMRYDDPEG
                     DYGRQKRQREVIQKVLKKILALNSISSYKKILSAVSNNMQTNIEISSKTIPNLLAYKD
                     SLEHIKSYQLKGEDATLSDGGSYQILTKKHLLAVQNRIKKELDKKRSKTLKTSAILYE
                     DYYGTTASNDSSTYSSTQENNYNTTPYSEAPPSYSGNTTYSSETNQTTHQSYYNSSTP
                     ASNYSSNTNTGQADSSGSVNNYNGAATPNPNTGTQPVPSQTNP"
     gene            complement(2292..2813)
                     /locus_tag="ACUBV3_001766"
     CDS             complement(2292..2813)
                     /locus_tag="ACUBV3_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HHU3848250.1"
                     /translation="MSKQFAVDFIEASREEASALLEFLNTVTEETDFILHTVSNQLSL
                     PEMETFIENTLITKNCICLIAKLKNKVIGLITIISQSDIEIEHIGDLFIAVQKDYWGY
                     GIGHILMEEAIEWASDNDITRRLELSVQGRNERAIHLYQKFSFEIDGLQTRGIKRENG
                     EFLDIYRMSKLID"
     gene            complement(2806..3249)
                     /gene="tsaE"
                     /locus_tag="ACUBV3_001767"
     CDS             complement(2806..3249)
                     /gene="tsaE"
                     /locus_tag="ACUBV3_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HHU3848251.1"
                     /translation="MFYTQNEEELIALGQKLGTVLKSGDIVLLTGNLGAGKTTLTKGI
                     AKGLDIKQMIKSPTYTIVREYEGRVPLYHLDVYRIGDDPDSIDLDDFLFGQGVTVIEW
                     GELLSDNLINNYLEIVITRSNQGRQVQLEAYGHRAREIIEAIQDV"
     gene            complement(3398..4819)
                     /locus_tag="ACUBV3_001768"
     CDS             complement(3398..4819)
                     /locus_tag="ACUBV3_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015851 - nucleobase transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HHU3848252.1"
                     /translation="MEKFFKLKEHGTTIRTEITAGLTTFFAMSYILFVNPAILSQTGM
                     PAQGVFLATIIGAVVATSVMAFYANLPYAQAPGMGLNAFFTYTVVFALGYTWQEALAM
                     VFICGLISLIITLTKVRKMIIESIPTTLKSAITAGIGTFLAYVGIKNAGFLKFSIDPG
                     TYDVVGKGAAKGLATITANSSATPGLVSFDNPAILLSLIGLSITIFFIVKGIRGGIIL
                     SILTTTLLGILMGVVKLDAINWEATNLSASFRDLKQVFGVALGEKGLISLFSNPSRLP
                     SVLMAILAFSLTDIFDTIGTLIGTGEKVGILATTGDNHESKSLDKALYSDLIGTTFGA
                     ICGTSNVTTYVESAAGIGAGGRTGLTALVVAGLFAISSFFSPLVSIVPSQATAPILVI
                     VGIMMLSNLKDIKWDDMSEAIPAFFTSLFMGFTYSITYGIAAGFLTYTLAKVIKGQAK
                     DIHVVLWILDILFILNFISLAIL"
     gene            complement(4922..>5242)
                     /locus_tag="ACUBV3_001769"
     CDS             complement(4922..>5242)
                     /locus_tag="ACUBV3_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1361 domain-containing protein"
                     /protein_id="HHU3848253.1"
                     /translation="YVSSILFGFLSGIESFSVIMRKFRISNIFLRWGIIGIVSFVSSF
                     GIHIGRYARLNSWDILTKPQVVINELLAVPSRDSFHFILGFTFLQVLCLIFMDYSAGK
                     KLEK"
BASE COUNT         1787 a         1005 c          780 g         1670 t
ORIGIN      
        1 acttactcaa taataaacat cattaaaaat tgcaataccc tttaggtcat gctaagataa
       61 gtatgatggt aaatatagat atcacaatca atacaggcta cttttatcca cctgtacgac
      121 ttatgaccta tcaaacgata aaaatttacc attccttaat agctacccaa taaatttttc
      181 aacatgaaag gaattagtta tggataattg tattttttgt aaaatcatca gtggagaaat
      241 ctcttcatca aaagtttatg aagatgatga agtattagct tttctcgaca ttacacaaac
      301 tacgactggg cacacccttc ttattcctaa aaaacatgtt cgaaatgtgt tagagatgga
      361 tgaaaaaact gcacagataa cctttgaacg cttgcctaaa gtggcgcgtg ctgtccaagc
      421 tgctacaaaa gccaaaggaa tgaatattat taacaacaat gaggaaattg ctggacaaac
      481 tgtttttcat gcacacgtcc accttgttcc ccgctttgat gaatcagacg gtatcaaaat
      541 tcactacact actcatgaac ccgactttga ggcacttgca aaattagcca aagaaattag
      601 aaaagagatt caattatgaa aataggtcgt ttaattgcac taggtttagt tagtttagga
      661 gcgcttgaac tttacaaaaa tagaaaaact atcaaggatt cctatcaaaa tacaaaaaat
      721 gagacagatt cagctaaatt aaaattagaa cgtatcaaaa atgatcttgc tattattagt
      781 caggaaaaag aaaaaatcag attaatttct caagaactca atcataaatt ccaagtgttt
      841 aataaagata ttcaacctcg tttagaagaa attaatcaac gcatggctaa atatcaagag
      901 aaagacgaat aagccgacgg tataaaaata tctactctaa atataaaaag agcaaataat
      961 gctctttttt atattttatg gattagtttg acttggtact ggttgcgttc ctgtgtttgg
     1021 attaggcgtt gcagccccgt tataattatt aacacttcca cttgaatcag cctgacctgt
     1081 gttagtgtta ctgctatagt tactagcagg agtgctacta ttatagtaac tttgatgagt
     1141 tgtttgatta gtctcagaac tataagtagt attaccactg taacttggtg gtgcttctga
     1201 ataaggtgtt gtattataat tattctcttg tgttgatgaa taagtagaag aatcattact
     1261 agcagtagta ccatagtaat cttcatatag aatcgcgctt gtcttcagag ttttactacg
     1321 ctttttatcc agctctttct taattctatt ttgaactgca agtagatgtt tcttagttaa
     1381 aatttgataa gagccaccat ctgatagagt agcgtcttca cccttcaact gataagattt
     1441 aatatgttcc aatgaatctt tataagctaa caaattagga atcgtttttg atgatatctc
     1501 aatattagtt tgcatgttat tacttactgc ggaaagaatt tttttgtatg aactaatact
     1561 atttaacgcc aatatttttt taaggacttt ttgaattact tcacgttgtc ttttttgacg
     1621 cccataatct ccctctggat catcatagcg catacgagaa taaacaagtg cttgttctcc
     1681 atttatttta tgtgtccctg gttcaacaac agccttgtac tctggttcat tggcagcaat
     1741 tgatattgga aagtcaaatt tattagttac tgttatacca ccaacagcat tgaccaaatc
     1801 aactaatcct tgcatattaa tttgcataaa gtaatcaaca ttaatatcta ataagtcttg
     1861 aacagtcatc aatgccattt ccgcaccacc agaagcatag gctgcattta gctttgcttc
     1921 tacgccagtc tgtccattat ttttgggacc actcaattta atcaatacgt cacgttctaa
     1981 gcttgtcatc gttgttttat tagttttagg atttatagtg actaagatca tagaatcgct
     2041 atttcctgac cacttagatt ttcgatgctc tgaacctgtg tccacaccca ttaatagtat
     2101 tgaaaacggc tttgtttctt caatagcatg actttttgat ttagctagct taaaatcttt
     2161 aaaagtctta gacaattcat tagtcgaaaa attataagct gaggcaacat agactcccaa
     2221 tgcaattact gttgttaaaa taattgcaga aaacattaag gttatttttt tccaaatttt
     2281 catgtatatt cctaatctat caacttactc attctgtaaa tgtctagaaa ttctccattt
     2341 tctcttttaa tacctcttgt ttgaagccca tctatttcaa aactgaattt ttggtagagg
     2401 tgaatagcac gttcattacg tccttggact gatagttcta agcggcgtgt aatatcgtta
     2461 tctgacgccc attctattgc ttcttccatg agaatatgac caataccata cccccaataa
     2521 tccttctgaa cagcaataaa aagatcccca atgtgttcaa tttcaatatc agactgacta
     2581 atgattgtta tcaagccgat cactttattt tttaatttag caatcaaaca aatgcaattt
     2641 ttcgttatta aggtattttc aataaacgtt tccatttctg gcaaagatag ttgattgctc
     2701 accgtatgca aaataaaatc tgtttcctca gtaacagtat ttaaaaattc aagaagcgca
     2761 gatgcttcct ctctcgaagc ttctataaaa tcaacagcaa attgtttaga catcttgaat
     2821 cgcctcaata atttctcttg cgcgatgccc ataagcttct aactgaactt gacgtccttg
     2881 attgctcctc gtaataacaa tttctaagta gttattgata aggttatcac taagcaactc
     2941 tccccattca ataactgtta ctccttgacc aaacaagaag tcatctaagt caatagaatc
     3001 aggatcatct ccaatacgat aaacatccaa atggtaaagt ggtacacgcc cttcatattc
     3061 tctaacaata gtgtaagttg ggctcttaat catttgtttt atgtctaatc cctttgcaat
     3121 accttttgta agagttgttt tgcctgcacc taaattacca gtcaataata cgatatctcc
     3181 tgactttaaa acagtaccca gtttttgccc tagggcaata agctcttctt cattttgagt
     3241 ataaaacata gagatattat accagaaata tctctgtctt ccaacagttt gctgattctg
     3301 acagccccat agcatatatt tcttcacttt tcttaagaaa aaaggaaacc aggctacaaa
     3361 tctgatttcc ttttatctta tttatcaaac tataatatta caaaatagcc aagctgataa
     3421 agtttagaat aaataatata tctaaaatcc acagtaccac atgaatatct tttgcttgtc
     3481 ctttgatgac tttagctagt gtataggtca agaaaccagc tgcaatacca taagtaatgc
     3541 tataagtaaa ccccataaaa agagaagtaa agaaagctgg gatagcttct gacatatcat
     3601 cccatttgat atcttttaaa tttgaaagca tcataatacc aacaataacc aatattggtg
     3661 cagtagcttg actcggaacg atagatacta gtggactaaa aaaactagat atagcaaaca
     3721 aaccagcaac tactaaggct gtcaatcctg tacgaccacc agctccgatt cctgctgctg
     3781 actcaacata ggttgtaacg ttagatgttc cacaaatagc accaaaagtt gtcccaatta
     3841 agtctgaata gagtgctttg tctaaactct tagattcatg attatcacca gtcgttgcta
     3901 agataccaac tttttctccg gtaccaatga gcgttccaat ggtatcaaaa atatcagtca
     3961 gtgaaaaagc aagaatggcc attaatacac ttggtagacg tgaaggatta gaaaataaag
     4021 aaattaatcc tttctctcct aatgcaactc caaatacttg cttcaaatct ctaaaagagg
     4081 ccgagaggtt agttgcttcc caattaatag catctaactt aaccactccc attaaaatac
     4141 ctaagagggt cgttgttaat atcgatagaa taatccctcc acggataccc tttacaataa
     4201 agaagatggt aatgcttaaa ccaatcaaag ataataaaat tgctggatta tcaaacgaca
     4261 ctagccctgg cgttgctgaa gaatttgctg taatagtagc caatcctttc gcagcaccct
     4321 ttccaacaac atcatacgtc ccaggatcaa ttgaaaattt caaaaaacca gcatttttaa
     4381 tcccaacgta tgctaaaaaa gttccgattc ctgctgtaat ggctgatttt aaagttgttg
     4441 ggatagactc aataatcatc tttctaactt tcgttaaagt aataatcaga gaaatcaaac
     4501 cacaaataaa taccatagct aaggcttctt gccatgtata tcccaatgca aagacaactg
     4561 tatatgtaaa gaaagcattt aatcccatcc caggagcttg tgcataggga aggttagcat
     4621 agaaagccat aacagatgta gctacaacgg caccaataat cgtcgctaag aaaactcctt
     4681 gtgctggcat cccagtctga gataaaatag ctggatttac aaacaaaatg tacgacattg
     4741 caaagaaagt cgttagacca gcagtgattt cagtccgaat tgtcgtcccg tgttccttta
     4801 gcttaaaaaa cttttccatt ttaaaatcgg attctccttt attttttgtt attttacact
     4861 tttaaaaaaa ttaatcgttc atatatttac gaacgattaa tatatattaa caaaaaataa
     4921 ttcacttttc aagctttttc cctgcagaat aatccataaa aataagacac aatacctgta
     4981 aaaatgtaaa tcctaaaata aagtggaatg aatccctact cggaaccgct aaaagttcgt
     5041 taattaccac ttgtggcttc gttaagatat cccatgaatt tagacgggca taacgaccaa
     5101 tatgaatacc aaaacttgaa acaaatgata caattccaat aattccccat cttaaaaata
     5161 tattgctaat tctaaattta cgcataatca cagaaaaact ttcgatacct gataaaaaac
     5221 caaacaagat actagataca ta
//
LOCUS       DBJYSH010000119         5091 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.119, whole genome shotgun sequence.
ACCESSION   DBJYSH010000119 DBJYSH010000000
VERSION     DBJYSH010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 5091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5091
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.119"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            <1..845
                     /locus_tag="ACUBV3_001770"
     CDS             <1..845
                     /locus_tag="ACUBV3_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HHU3848254.1"
                     /translation="LNIGSLIVAFGFNAFFLHNHIVTGGISGLAVSFHEVFGWDSAFF
                     IMIINIPLLLLCYFGLGKQTFLKTVYGSWIFPVFIKLTQSVPTLTHNPLLAALFGGVI
                     VGCGLGIVFWSDSSTGGTGIIIQFLGKYTPISLGQGVILIDGLVTIVGFLAFDSDTVM
                     FSIIGLITISYIINAIQTGFTTLSTVLIVSQEHQKIKTYINTVADRGVTEIPVKGGYS
                     GTNQIMLMTTIAGYEFAKLQEAIAEIDETAFITVTPTSQASGRGFSLQKNHGRLDEDI
                     LMPM"
     gene            911..2530
                     /locus_tag="ACUBV3_001771"
     CDS             911..2530
                     /locus_tag="ACUBV3_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HHU3848255.1"
                     /translation="MLTVSDVSLRFSDRKLFDEVNINFTAGNTYGLIGANGAGKSTFL
                     KILAGDIEPTTGHIALGPDERLSVLRQNHFDYEDERVIDVVIMGNETLYSIMKEKDAI
                     YMKEDFSDEDGVRAAELEGEFAELGGWEAESEASQLLQNLNISEELHYQNMSELANGD
                     KVKVLLAKALFGKPDVLLLDEPTNGLDIQSITWLEDFLIDFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLFVGNYDFWKESSELAARLQADRNAKAEEKIKQLQEFVARFSANA
                     SKSKQATSRKKMLDKIELEEIVPSSRKYPFVNFKAEREMGNDLLTVENLSVTIDGEKI
                     LDNISFILRPGDKTALIGQNDIQTTALIRALMGDIEYEGTIKWGVTTSRSYLPKDNSR
                     DFASGESILEWLRQFASKEEDDNTFLRGFLGRMLFSGDEVNKSVNVLSGGEKVRVMLS
                     KLMLLKSNVLVLDDPTNHLDLESISSLNDGLKDFKESIIFASHDHEFIQTLANHIIVL
                     SKNGVIDRIDETYDEFLENTEVQAKVAQLWK"
     gene            2653..>5091
                     /locus_tag="ACUBV3_001772"
     CDS             2653..>5091
                     /locus_tag="ACUBV3_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000154428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HHU3848256.1"
                     /translation="MTINHYKLRIPYYTISFLLPFIIIVCILFTKNIYWGSPTTILAS
                     DGFHQYVIFNQALRNILHGSNSLFYTFTSGLGLNFYALSSYYLGSFLSPIVYFFNLKN
                     MPDAIYLLTICKIGLIGLSMFVTLCKRHCKVNRVLLLVISTCYSLMSFSISQIEINMW
                     LDVFILIPLVVLGVDQLLWERKPILYFLSLTALFIQNYYFGFMTAIFTSLYFIVQITR
                     NTDSKVAFKQFLHFTFLSLLAGMTSSIMILPTYFDLTTHGEKLTKVSKMFTENSWYMD
                     LFAKNMIGAYDTTKFGSIPMIYVGLLPLLLSLLYFTIKEVPRRTRLAYGFLIIFVIAS
                     FYITPLDLFWQGMHAPNMFLHRYSWVLSVLICLLAAECLEYLDNISWKKILGVNLILV
                     SGFIITFLFKKHYHYLNLELLLLTLTFLSAYIILTISFVSKQIPKLVFYPFLIGFVVL
                     EMTLNTFYQLNSLNDEWIFPSRQGYAKYNHSISKLVRKTERNNSTFFRTERWLGQTGN
                     DSMKYNYNGISQFSSIRNRSSSQVLDRLGFKSDGTNLNLRYQNNTLIADSLFGVKYNL
                     TEYPFDKFGFIKKAQDKQTILYKNQFASQLAILTNQVYQDKPFTVNTLDNQTTLLNQL
                     SGLKETYFEHLIPNSVSGQTTLNKQVFVKKNKQGNTEITYNITIPKNSQLYVSMPFIN
                     FNNEENKIVQISVNNGPFVPNTLDNAYSFFNIGSFAENSRIKVKFQFPHNDQVSFPIP
                     HFYGLKLEAYQKAMTVINKRKVKVRTDHNKVIANYTSPNRSSLFFTIPYDRGWKAYQN
                     NKEIKIFKAQKGFMK"
BASE COUNT         1637 a          838 c          842 g         1774 t
ORIGIN      
        1 tattaaatat tggatctcta attgttgcat ttggtttcaa cgctttcttt cttcataatc
       61 atattgtcac gggggggatt agtggtttgg cagttagttt tcatgaagta tttggttggg
      121 attctgcttt ttttattatg attatcaata ttccattgct ccttctttgc tactttggct
      181 taggtaaaca aaccttttta aaaactgtct atggttcttg gatttttcct gtttttatta
      241 agttaacaca aagtgtacca actttgaccc acaacccact cctcgcagca ctttttggag
      301 gtgttattgt aggatgtggt ttggggattg ttttttggag cgactcttca actggtggaa
      361 cggggattat cattcaattc ttaggaaaat atactcctat aagccttgga caaggggtta
      421 tattgattga tggacttgtt acaattgttg gtttcctagc ttttgacagt gatacggtta
      481 tgttttctat tattgggttg ataactatta gttatattat taatgctatc caaactggat
      541 ttacaacctt aagcactgtc ttaatcgttt ctcaagagca ccaaaaaatt aagacatata
      601 tcaatactgt cgcagataga ggagtaacag aaattcccgt taaaggggga tattctggaa
      661 ctaatcaaat catgcttatg acaactattg ctggttatga gtttgctaaa ttacaagagg
      721 caatagcaga aattgacgaa acagccttca taacagtaac tccaacatca caagcttctg
      781 gacgtggatt tagtcttcaa aaaaatcatg gacgtcttga tgaagacatt cttatgccaa
      841 tgtaatcgat tgataattaa agctttttct agtagaatag cagatagaat atacacaaga
      901 ggtaacaaaa ttgcttacag tttcagatgt ttcactacgt tttagtgatc gcaaattatt
      961 tgatgaagtt aacattaact ttacagcagg aaatacctat ggtcttatcg gagcaaatgg
     1021 cgctggtaaa tcaactttcc ttaaaatttt agctggtgat attgaaccaa caacaggaca
     1081 tattgcactt ggcccagatg aacgcctttc tgtacttcgc caaaatcatt ttgattatga
     1141 agatgagcgc gtcattgatg tcgttatcat gggtaatgaa acactttata gtatcatgaa
     1201 agaaaaggat gctatctaca tgaaagaaga tttttctgat gaagatggtg ttcgcgctgc
     1261 ggaattagaa ggagaatttg ctgaactcgg tggatgggag gctgaaagtg aggcttctca
     1321 attactccaa aatctaaata tttcagaaga gcttcattat caaaatatga gtgaactagc
     1381 taatggagac aaagttaaag ttctccttgc aaaagctctc tttggtaaac cagatgtact
     1441 tctccttgac gagcctacca atggacttga tatccagtct attacttggt tagaagattt
     1501 cttgattgac tttgaaaata cagttattgt agtttcccac gaccgtcatt tcttgaataa
     1561 agtatgtaca catatggctg accttgattt tggaaaaatt aaactgttcg tcggtaacta
     1621 tgatttctgg aaagaatcat cagaattagc agctcgttta caagctgatc gtaatgctaa
     1681 agcagaagaa aaaattaaac aacttcaaga attcgttgca cgattttcag cgaatgcttc
     1741 aaaatcaaaa caagcaactt ctcgtaaaaa aatgcttgat aaaattgaac ttgaagagat
     1801 tgtaccatca agccgtaaat acccattcgt taactttaaa gccgaacgcg aaatgggaaa
     1861 tgatttattg accgttgaaa atctctctgt cactattgat ggggaaaaaa tccttgataa
     1921 tatcagtttc attcttcgtc ctggtgataa aactgcactt attggacaaa atgatatcca
     1981 aactactgca cttattcgag cattgatggg ggatatcgaa tatgaaggaa caatcaaatg
     2041 gggagtaaca actagtcgtt cttaccttcc aaaagacaat tcacgagatt ttgcttctgg
     2101 cgagtcaatc ttagaatggc ttcgtcaatt tgctagtaaa gaagaagacg ataatacctt
     2161 cttacgtggt ttcctaggtc gtatgctttt ctctggagat gaggttaaca aatctgttaa
     2221 tgtcttatct gggggtgaaa aagtacgcgt tatgctttcg aaattaatgc tattaaaatc
     2281 taatgttctt gttcttgatg atccaacaaa ccacttagat ttggaatcta tttcaagtct
     2341 caacgatgga ctaaaagatt tcaaagaatc tattattttt gcaagtcatg accacgagtt
     2401 tattcaaact ttagcgaacc atattattgt tctttctaaa aacggagtta tcgatcgtat
     2461 cgatgaaact tacgatgaat ttcttgaaaa taccgaagta caagctaagg tcgctcaact
     2521 ctggaaataa tcctttggca tatctctaac ctaataaacg tgtcaagatt gaaaatctta
     2581 aaactatatt aatcatttaa ataaaaaggt tggaacaaat agtgtccagc ctttttatct
     2641 taggagatat atatgacaat taaccactat aagcttcgta taccttacta tacaataagc
     2701 tttctacttc ccttcataat catagtctgt atcctcttta ctaagaatat ttattgggga
     2761 agtccaacaa ccatattagc aagcgatggc tttcatcaat atgttatctt taatcaagca
     2821 ttaagaaata ttcttcatgg ttcaaacagc ttattctata cctttacaag cggtttaggt
     2881 ttaaattttt atgctctgag tagttactat ttgggaagtt ttttatcccc aatagtttat
     2941 ttctttaacc taaaaaatat gcctgatgct atctaccttt taacaatatg taaaataggg
     3001 ttgataggtt tgtcaatgtt tgtcactctt tgtaaacgtc attgtaaagt taatcgtgtc
     3061 ttacttttag taatttctac ttgctatagc ttaatgagtt tctcaattag ccaaattgaa
     3121 attaatatgt ggctagatgt atttattcta attccccttg ttgttttagg agttgatcag
     3181 ctactatggg aaagaaaacc tatactttat ttcctctctc tgacagcctt attcatccaa
     3241 aactattatt ttggttttat gactgccatc tttactagcc tttacttcat cgtacaaata
     3301 acccgtaata ctgatagtaa ggttgcattt aaacaatttc ttcattttac ctttttatct
     3361 cttttagctg gtatgactag tagcattatg attttgccaa cttattttga tttaactact
     3421 catggtgaaa agttgacaaa ggtatcgaag atgtttacag aaaattcttg gtacatggat
     3481 ttgtttgcta aaaatatgat tggagcctat gatacaacaa aatttggctc tattcctatg
     3541 atttatgtag gtttacttcc tttactcctt tctttacttt actttacaat aaaggaagtt
     3601 cctcgaagga ctagattagc atatggattt ttaataatat ttgttattgc tagtttttat
     3661 ataacacctc tagatttatt ttggcaaggg atgcatgctc caaatatgtt tctccatcgc
     3721 tactcttggg tactttcagt acttatctgt ctgctagctg ctgaatgcct tgagtattta
     3781 gacaacattt cttggaaaaa gattttaggg gtcaatttaa ttttagtaag tggtttcata
     3841 atcacgtttt tatttaaaaa gcattatcat taccttaatc ttgaattact tcttttaacc
     3901 ttaactttcc tctcagctta tattattcta actattagtt ttgtcagtaa gcaaatccct
     3961 aaattagttt tttatccctt tcttattggt tttgttgtat tagagatgac attaaacacc
     4021 ttttaccaac taaatagtct taacgatgaa tggatatttc catctaggca aggatatgct
     4081 aaatataatc acagcattag caaacttgtc agaaagactg agagaaataa ctctacattc
     4141 ttccgtacag agcgctggtt aggacaaacc ggcaacgact ctatgaaata taattataat
     4201 ggtatttcac aattttcatc tattagaaat cgttcttcaa gtcaagtgct tgatagactt
     4261 ggctttaaat cagatggtac aaatttaaat ttacgttatc aaaataatac tttgatcgca
     4321 gatagtctat ttggtgtgaa gtataacctt acagaatatc cttttgataa atttggtttt
     4381 atcaaaaaag ctcaagataa acaaaccatt ctttacaaaa atcagtttgc cagtcaatta
     4441 gctatactaa cgaatcaagt atatcaagat aaaccattta ctgtgaatac tttggataat
     4501 cagacaactc ttttaaatca attaagtggt ttgaaagaga cgtattttga gcatcttatt
     4561 ccaaatagcg tttctgggca aaccactctt aataaacaag tatttgttaa aaagaataaa
     4621 caagggaata ctgaaattac ctataacata actatcccta aaaacagtca gttatatgtt
     4681 agtatgccgt ttataaattt taataatgag gaaaataaaa ttgttcaaat ctccgttaat
     4741 aatggaccat ttgtacctaa tactcttgat aatgcttatt cattttttaa tattggttcc
     4801 tttgctgaaa acagtcgtat caaagtaaaa tttcaatttc cacacaatga tcaagtttct
     4861 ttcccaattc ctcactttta tgggctcaag ttagaagctt atcaaaaagc tatgactgtt
     4921 attaataaac gaaaagtaaa agtaagaact gatcacaata aagttattgc taattataca
     4981 agtcctaata gaagttctct tttcttcact attccctatg ataggggttg gaaagcttat
     5041 caaaataata aagaaataaa aattttcaaa gctcagaaag gttttatgaa a
//
LOCUS       DBJYSH010000120         5039 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.120, whole genome shotgun sequence.
ACCESSION   DBJYSH010000120 DBJYSH010000000
VERSION     DBJYSH010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 5039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5039
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.120"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            complement(1..2265)
                     /gene="glgP"
                     /locus_tag="ACUBV3_001773"
     CDS             complement(1..2265)
                     /gene="glgP"
                     /locus_tag="ACUBV3_001773"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000197732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan family
                     phosphorylase"
                     /protein_id="HHU3848257.1"
                     /translation="MTRNFTTYVGQQGKVLSELTNEEIYVELLNFVKEEAAAKSKNSS
                     QRKVYYISAEFLIGKLLSNNLINLGIYKDVKKELELVGKSIAEIEDVEPEPSLGNGGL
                     GRLASCFIDSISSLGINGEGVGLNYHCGLFKQVFRNNQQEAEANYWIENNSWLVPTDI
                     SYDVPFRDFTLKSRLDRIDVLGYKKDTKNYLNLFDIDGLDYNLIEKGITFDKTEIKKN
                     LTLFLYPDDSDKNGELLRIYQQYFMVSNAAQLLIDEAIERGSNLHDLAEYAYVQINDT
                     HPSMVIPELIRLLTEKHGFEFDEAVSVVRNMVGYTNHTILAEALEKWPLEYLNEVVPH
                     LVTIIKKLDQMIREEQTNPEVQIIDEAGRVHMAHMDIHFSTSVNGVAALHTEILKNSE
                     LKVFYDIYPDKFNNKTNGITFRRWLEFANQDLADYLKELIGDSYLTDATQLEKLLTYA
                     DSNEVHDKLAAIKFKNKLALKRYLKENKGIELDEYSIIDTQIKRFHEYKRQQMNALYV
                     IHKYLEIKRGHFPSRKLTVIFGGKAAPAYTIAQDIIHLILCLSELINNDPEVNKYLNV
                     HLVENYNVTVAEKLIPATDISEQISLASKEASGTGNMKFMLNGALTLGTMDGANVEIA
                     ELAGKENIYTFGKDSDTIINLYETSGYRSKDYYDKDKVIREAVDFIISDDIVSLGNAE
                     RLKRLHDELVGKDWFMTLIDLKEYIAVKEQVLADYEDYESWNKKVIHNIAKAGFFSSD
                     RTIEQYNQDIWHSN"
     gene            complement(2277..3773)
                     /gene="malQ"
                     /locus_tag="ACUBV3_001774"
     CDS             complement(2277..3773)
                     /gene="malQ"
                     /locus_tag="ACUBV3_001774"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HHU3848258.1"
                     /translation="MKKRASGVLMHITSLPGDLGIGTFGREAYAFVDFLVETDQKFWQ
                     ILPLTTTSFGDSPYQSFSAVAGNTHLIDFDLLTLEGFISKDDYQNISFGQDPEVVDYA
                     GLFEKRRPVLEKAVKNFLQEERATRMLSDFLQEEKWVTDFAEFMAIKEHFGNKALQEW
                     DDKAIIRREEEALAGYRQKLSEVIKYHEVTQYFFYKQWFELKEYANDKGIQIIGDMPI
                     YVSADSVEVWTMPELFKLDCDKQPLAIAGVPADDFSDDGQLWGNPIYNWDYHKESDFD
                     WWIYRIQSGVKMYDYLRIDHFKGFSDYWEIRGDYQTANDGSWQPAPGPELFATIKEKL
                     GDLPIIAENLGYIDERAERLLAGTGFPGMKIMEFGFYDTTGNSIDIPHNYTENTIAYA
                     GTHDNEVINGWFENLTVEQKAYAENYMRRLPNEPITETVLRTLYATVSQTTITCMQDL
                     LDKPADSRMNMPNTVGGNWQWRMRKEDLTENRKAFLKEITTIYNRGNK"
     gene            complement(3894..4922)
                     /locus_tag="ACUBV3_001775"
     CDS             complement(3894..4922)
                     /locus_tag="ACUBV3_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HHU3848259.1"
                     /translation="MVTIKDVAAKAGVNPSTVSRVLKDNESISSKTKERVKKAMEELG
                     YVPNVAAQMLASGLTQNIGVIFPPLVLPDRLSEPFFMEILSTLTNEAKEHGFTVSIAT
                     SMSSEELLEQVKLMHLQKRVDGFIVLYSDQKDPIREYLLKYKLPFVVVGAANGYENEI
                     TYIDNDNQLMGKSAIDYLYKKGHKSIQFVTDDLNSEVSEERYLGYFKGARKLGLNQKP
                     ALLFDRGNPQVLEEFINRVKEEETTALIVIGDTVSVRVMQFLSFYKLKVPDDISIMTF
                     NNSLFSHLIHPYLSTFDINVNNLGRTSVRRLIDIIKSPDKVFSETIIVPFTLEERESV
                     RDISKNNN"
BASE COUNT         1585 a          973 c          750 g         1731 t
ORIGIN      
        1 ttaatttgaa tgccaaatat cttgattata ttgttcgatt gtacgatctg atgagaagaa
       61 ccctgcttta gcaatattgt gtataacttt cttattccat gattcataat cttcataatc
      121 agcaagcact tgttctttaa cagcaatgta ttctttcaaa tcaatcaatg tcatgaacca
      181 atctttacct actaactcat catgcaatct ctttagtcgt tctgcattac cgagtgacac
      241 tatatcatca ctaatgataa aatcaacagc ttcacgaatt actttgtctt tatcatagta
      301 atcttttgaa cgataaccag atgtttcata aagattaatg atagtatcag agtctttacc
      361 aaatgtgtag atgttctctt tgccagctaa ttcagctatt tcaacatttg ccccatccat
      421 agtaccaaga gtaagagcac cattcaacat aaatttcatg ttacctgtac cagatgcttc
      481 tttggaagcc aatgaaattt gttccgaaat atctgttgca gggattaact tttccgctac
      541 tgtcacattg taattttcta ctaaatggac attaaggtat ttattaactt ctggatcatt
      601 attaattaat tctgataagc agagaattaa gtgaatgata tcctgtgcaa tagtataggc
      661 tggagcagct ttaccaccaa aaataactgt taatttacgt gatgggaaat gcccacgttt
      721 aatttctaag tatttatgaa taacatataa agcattcatt tgttggcgct tatattcgtg
      781 gaatcgtttg atttgtgtat caataattga gtattcatcc aattcaatac ctttattttc
      841 cttaaggtaa cgtttaagag ccagtttgtt tttaaattta atagcagcta atttatcatg
      901 aacttcattg ctatcagcat aagttaacaa tttttcgagc tgagtcgcat cagtaagata
      961 gctatcccca attagttctt tcaagtaatc tgctaaatct tggttagcaa actccaacca
     1021 gcgacggaat gtaataccgt ttgtcttatt gttaaactta tctgggtaaa tatcatagaa
     1081 aacctttaat tcactatttt taaggatttc tgtatgtaat gctgctacac cattaacgct
     1141 tgttgagaag tggatatcca tatgcgccat gtgaacacgt ccagcttcat caataatttg
     1201 aacctctgga tttgtttgct cttcacgaat catttgatct aattttttaa tgattgttac
     1261 taggtggggt actacctcat taagatactc taaaggccat ttttcaagag cttctgcaag
     1321 gatggtatgg tttgtatagc caaccatatt gcgaacaact gacacagctt cgtcaaattc
     1381 aaaaccatgt ttttctgtca ataaacgaat caactcaggg atgaccatag atgggtgagt
     1441 gtcattaatt tgaacataag catattcagc taaatcatga aggtttgaac cacgctcgat
     1501 tgcttcatca attaataatt gagcagcatt tgagaccatg aaatattgct gatagatacg
     1561 taataattct ccatttttat ctgaatcatc tggatacaag aacaaggtta aattcttctt
     1621 gatttctgtc ttatcaaaag tgataccttt ttcaataagg ttataatcaa gtccatcaat
     1681 atcaaaaagg ttcaaataat ttttagtatc tttcttataa cctaatacat caattctatc
     1741 taggcgtgat tttaaagtaa agtcacggaa aggaacatca taagaaatat ctgttggtac
     1801 taaccaagaa ttattctcaa tccagtagtt agcttctgct tcttgttgat tgttgcgaaa
     1861 tacttgtttg aaaagcccac agtgatagtt taaaccaaca ccttcaccat taatacctaa
     1921 agaagaaatc gagtcaataa aacatgatgc taaacgccct aaaccaccat taccaagtga
     1981 tggttctggc tctacatctt ctatttcagc aattgattta cctacaagtt ctaactcttt
     2041 tttgacatct ttataaatcc ctaagttaat caaattattt gaaagtaatt taccaattag
     2101 aaactcagct gaaatatagt ataccttacg ttgagagcta ttttttgatt tagcggcagc
     2161 ttcttctttt acaaaattta acaattcaac gtaaatctct tcatttgtta attctgataa
     2221 tacttttcct tgttgtccaa cgtaagttgt aaaatttctt gtcatgataa tattccttat
     2281 ttattccctc tattataaat agttgtaatc tcttttaaga atgctttgcg attttctgtt
     2341 aagtcttctt ttctcatacg ccattgccag ttacctccaa cggtatttgg catgttcata
     2401 cggctatctg caggtttatc tagtagatct tgcatacatg taatcgttgt ttgactaacg
     2461 gttgcataca aggtacgcag tacagtctca gtaatgggct catttggcaa acgacgcatg
     2521 taattttctg cataagcttt ctgttcgact gtaagatttt caaaccagcc attaataact
     2581 tcgttatcat gagttccagc atatgcaatt gtattttctg tgtagttatg aggaatatca
     2641 atactatttc ctgttgtatc ataaaaaccg aattccatga ttttcatacc tgggaaccct
     2701 gttccagcca gtaatctctc tgctctctca tcaatatagc ctaaattctc agcaataata
     2761 ggtaaatcac ctaatttttc tttgatagtt gcaaataatt ctggaccggg agctggttgc
     2821 catgagccat cattagctgt ttgataatct cctcgaattt cccaataatc tgaaaaacct
     2881 ttaaaatgat caatacgcaa atagtcatac atcttaacac cagattgaat acgataaatc
     2941 caccagtcaa aatctgactc tttgtggtaa tcccaattgt aaataggatt gccccaaagc
     3001 tgaccatcgt cagaaaaatc atccgctggt acaccagcaa tagcaagtgg ttgtttgtcg
     3061 caatctaatt taaacagttc aggcattgtc caaacttcta cactatcggc agaaacgtag
     3121 attggcatat caccgataat ttgaatccct ttatcattag catattcttt taactcaaac
     3181 cattgtttgt aaaagaaata ttgcgttact tcatgatatt ttatcacttc actaagcttt
     3241 tgacgatatc ctgctaaggc ttcttcttcg cggcgtataa tagccttgtc atcccattct
     3301 tgaagcgcct tattaccaaa atgttctttg atcgccataa attcagcaaa atcagttacc
     3361 catttttctt cttgcaagaa atcagatagc attctcgtag ctctctcttc ttgcaagaaa
     3421 tttttaactg ctttttctaa aactggacgg cgtttttcaa acagaccagc ataatcaaca
     3481 acttctggat cctgtccaaa actaatattt tggtagtcat ctttcgaaat gaaaccttca
     3541 agcgttagca aatcaaaatc aattaaatgt gtattaccag ctactgcaga aaaagactgg
     3601 taaggagagt ccccgaagct tgttgttgtt aagggaagaa tttgccaaaa tttttgatca
     3661 gtttcgacca aaaaatcaac aaaagcgtag gcttctcgtc caaatgttcc aatacctaaa
     3721 tcacctggta gagaagtgat gtgcattaag acaccacttg cacgtttttt catattgatt
     3781 ctccttttcg attacttact ttactcctcc atcttaacgc aagcgattgc ataagtcaag
     3841 cataatttaa aaaaacttct atcgaaaatt gatagaagtc tttatatcat agcttaatta
     3901 ttatttttag aaatatctcg gacactttca cgctcttcta aagtaaaagg cacaataata
     3961 gtctcactaa agactttgtc tggagattta atgatgtcaa tcagacgacg aacgcttgtc
     4021 cttcctagat tattaacatt aatatcaaat gtgcttaggt aaggatgaat cagatgtgag
     4081 aagagagaat tattaaaggt catgatagaa atgtcatctg gaacttttaa tttatagaaa
     4141 gaaagaaatt gcattactct aacagagaca gtatcaccaa taacaattaa agcagttgtt
     4201 tcttcttctt tgactctatt tataaattcc tctaaaactt gcggattgcc tctgtcaaat
     4261 aaaagagctg gcttttggtt taaacctaac tttctagcac ctttaaaata ccctaaatag
     4321 cgctcttctg atacttctga atttaaatca tctgtaacaa actggatgct tttatgtcct
     4381 tttttataga gataatcaat ggcagatttc cccatcagct gattatcatt atcaatataa
     4441 gtaatctcat tttcatagcc attagcagca ccaacaacaa caaagggcag tttatatttt
     4501 aataaatact ctctgatagg atctttttga tccgaataga gaacaataaa accatccact
     4561 cgtttttgta gatgcattaa ttttacttgt tctaacaact cctcagaaga catacttgtt
     4621 gcaatagaaa ctgtaaaccc atgctctttt gcttcattag ttaatgtaga aagaatctcc
     4681 atgaaaaagg gttcacttaa gcggtctggt aaaaccagtg gaggaaaaat aactccaata
     4741 ttttgcgtta aaccactagc caacatttgt gctgcaacat taggaacata gcccagctct
     4801 tccatagctt ttttcacgcg ctctttcgtc ttacttgaaa tagattcatt atcttttagt
     4861 acacgactta ctgtcgatgg attaacacca gctttggcag caacatcttt tatagttacc
     4921 atcctacgac ctcttcattc taatcatttt ataccttttg gtatagtcct ttgtctatta
     4981 tactctaatc taagaaaaaa acaaacgttt gccttctctg caaatgtaat actctcact
//
LOCUS       DBJYSH010000121         5030 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.121, whole genome shotgun sequence.
ACCESSION   DBJYSH010000121 DBJYSH010000000
VERSION     DBJYSH010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 5030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5030
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.121"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            complement(69..142)
                     /locus_tag="ACUBV3_001776"
     tRNA            complement(69..142)
                     /locus_tag="ACUBV3_001776"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(106..108),aa:Asn,seq:gtt)
     gene            complement(172..243)
                     /locus_tag="ACUBV3_001777"
     tRNA            complement(172..243)
                     /locus_tag="ACUBV3_001777"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(208..210),aa:Glu,seq:ttc)
     gene            435..508
                     /locus_tag="ACUBV3_001778"
     tRNA            435..508
                     /locus_tag="ACUBV3_001778"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:469..471,aa:Arg,seq:tct)
     gene            complement(580..1059)
                     /gene="rlmH"
                     /locus_tag="ACUBV3_001779"
     CDS             complement(580..1059)
                     /gene="rlmH"
                     /locus_tag="ACUBV3_001779"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HHU3848260.1"
                     /translation="MKLKIITVGKLKEKYLKEGVAEYQKRLNRFSKIETIELADEKTP
                     DKASISENQRILDIEGERILSKIGERDYVIGLAIEGKQLPSESFSHLIDQKMISGYST
                     ITFVIGGSLGLSQKVKKRADYLMSFGLLTLPHQLMKLVLMEQIYRAFMIRQGTPYHK"
     gene            1260..2489
                     /locus_tag="ACUBV3_001780"
     CDS             1260..2489
                     /locus_tag="ACUBV3_001780"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HHU3848261.1"
                     /translation="MKKKLVSSLLKCSLIIIVSFAGGAFASFVMNHNDNIPNGGVTKT
                     SKVNYNNITPTTKAVKKVQNSVVSVINYKQQESRSDLSDFYSHFFGNQGGNTDKGLQV
                     YGEGSGVIYKKDGKNAYVVTNNHVIDGAKQIEIQLADGSKAVGKLVGSDTYSDLAVVK
                     IPSDKVSNIAEFADSSKLNIGETAIAIGSPLGTEYANSVTQGIVSSLKRTVTMTNEEG
                     QTVSTNAIQTDAAINPGNSGGALINIEGQVIGINSSKISSTSNQTSGQSSGNSVEGMG
                     FAIPSNDVVKIINQLESNGQVERPALGISMAGLSNLPSDVISKLKIPSNVTNGIVVAS
                     IQSGMPAQGKLKKYDVITKVDDKEVVSPSDLQSLLYGHQVGDSITVTFYRGENKQTVT
                     IKLTKTSKDLAKQRANN"
     gene            2587..3360
                     /locus_tag="ACUBV3_001781"
     CDS             2587..3360
                     /locus_tag="ACUBV3_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000456088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HHU3848262.1"
                     /translation="MEYLETININHIAPNPYQPRLEFNTKELEELANSIKINGLIQPI
                     IVRPSAVFGYELVAGERRLRAAKLAKLESIPAIIKSYNNDDSMQLAIVENLQRSNLSP
                     IEEAKAYSQLLQKKSMTHEELAKYMGKSRPYISNTIRLLNLPPLITSAIEEGKLSSGH
                     ARALLSLPDASQQKDWYQRILTEDISVRRLEKLLKQEKKTNHKSLQNKDVFLKHQENE
                     LAQFLGSKVKLTINKDGAGNIKIAFANQEELNRIINTLK"
     gene            3559..4920
                     /gene="dnaA"
                     /locus_tag="ACUBV3_001782"
     CDS             3559..4920
                     /gene="dnaA"
                     /locus_tag="ACUBV3_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012657571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HHU3848263.1"
                     /translation="MTENEQLFWNRVLELSRSQIAPAAYEFFVLEARLLKIEHQTAVI
                     TLDNIEMKKLFWEQNLGPVILTAGFEIFNAEITANYVSNDLHLQETSFSNYQQSSNEV
                     NTLPIRKIDSNLKEKYTFANFVQGDENRWAVSASIAVADSPGTTYNPLFIWGGPGLGK
                     THLLNAIGNQVLRDNPNARVLYITAENFINEFVSHIRLDSMEELKEKFRNLDLLLIDD
                     IQSLAKKTLGGTQEEFFNTFNALHTNDKQIVLTSDRNPNQLNDLEERLVTRFSWGLPV
                     NITPPDFETRVAILTNKIQEYPYDFPQDTIEYLAGEFDSNVRELEGALKNISLVADFK
                     HAKTITVDIAAEAIRARKNDGPIVTVIPIEEIQIQVGKFYGVTVKEIKATKRTQDIVL
                     ARQVAMYLAREMTDNSLPKIGKEFGGRDHSTVLHAYNKIKNMVAQDDNLRIEIETIKN
                     KIR"
BASE COUNT         1771 a          871 c          851 g         1537 t
ORIGIN      
        1 agagtattct catcctattt tattaacaaa aaaacgccga caaatcaacg tttagatgtg
       61 tcttatcact ccggcagcag gactcgaacc tgcgacatca tgattaacag tcatgcgcta
      121 ctaccaactg agctatgccg gataaataaa aaactgcaaa gcagttatca atagtccgta
      181 cgggattcga acccgtgtta ccgccgtgaa aaggcggtgt cttaacccct tgaccaacgg
      241 accataggtt aggtgtttcc ttagtactct taatattata tcagaaaaaa aaggtttgtc
      301 tatacttttg ttcaataatt tttacaaaaa aattgaacct acttatgagc aaaaaaatat
      361 atttccaact cataaatagt acatatgaaa acttgacagc ctgcctaata ttttggtaga
      421 ataacagagt tgatggttcc atagctcagc tggatagagc attcgccttc taagcgaacg
      481 gtcgcaggtt cgaatcctgc tggaatcaga tattgaaagc ctagatagat tatctaggct
      541 ttttatttgt ttttagcagt tgtttcataa atggaacgat tatttgtggt aaggagtgcc
      601 ttgacgaatc ataaaagcac gataaatttg ttccatcaag actaatttca taagttgatg
      661 aggtaaggtt aacagtccaa aactcattaa ataatcagca cgttttttca ctttttgaga
      721 taaacccaaa ctaccaccaa taacaaaagt aatagtagag tagccggata tcattttttg
      781 atcgattaga tggctaaatg attcagatgg caattgtttc ccttctatag ctaaaccaat
      841 gacataatcc ctctcaccta ttttactcaa aatacgttcc ccttctatat ctaaaattct
      901 ttgattttca gatatactag ctttatcagg tgtcttttca tcagctagct caattgtctc
      961 aattttagaa aagcgattca gacgtttctg atactctgca acaccttctt taaggtattt
     1021 ctctttcaat tttccaactg taattatttt taatttcata gctacattat aacataatcc
     1081 acaatatttt cacaatttat ccacatgttt atccttacat atccccttat tttataggta
     1141 ttctataact ataatcaagg ttatccacaa gttgtggata tttccttttt ttagctttta
     1201 aggtataatt aagaagcttg tcataagatt agtataaaaa tttacggagg tagctatttg
     1261 tgaaaaaaaa attagtctca tcacttctaa agtgttctct aatcattatt gttagctttg
     1321 ctggtggagc atttgctagt tttgtcatga atcataatga caatattcca aatggtggtg
     1381 tcactaaaac tagtaaagta aattataata acataacgcc tacaacaaaa gctgttaaaa
     1441 aggtacaaaa tagtgttgtt tctgttatca attataaaca acaagagagt cgttctgacc
     1501 tatcagactt ctatagtcat ttttttggta atcagggggg caacactgat aagggcttac
     1561 aagtttacgg tgaaggctct ggagtcatct ataaaaaaga tggtaaaaat gcctatgttg
     1621 tcactaataa ccacgtcatt gatggggcta aacaaattga aattcaacta gctgatggct
     1681 caaaagcagt tgggaaactt gttgggtcag atacctactc tgatttagcc gtcgtcaaaa
     1741 ttccatcaga taaggtttca aatattgcag aatttgctga ttcatcaaaa ctcaacattg
     1801 gtgaaactgc tatagcgatc ggaagccctc ttggaactga gtatgcaaat tctgtaactc
     1861 aaggtattgt atctagttta aaaagaactg taacaatgac taatgaagaa ggacaaacag
     1921 tttctacaaa tgctatccag actgatgctg ctatcaatcc tggtaattca ggtggagcac
     1981 ttatcaatat tgaaggacag gttattggaa ttaattctag taaaatttct tctacatcaa
     2041 atcaaacctc aggacaatcg tcaggaaata gcgttgaagg tatgggattt gccattcctt
     2101 caaatgatgt tgttaagatt atcaatcaac ttgagagtaa cggacaagta gagagacctg
     2161 ctctaggtat ttctatggct ggattaagta atttaccatc cgatgttatt agtaaactga
     2221 aaatcccaag taatgttact aatggtattg tagtagcatc tatccaatct ggcatgccag
     2281 ctcaaggcaa actaaagaaa tacgatgtca ttactaaagt tgacgataaa gaagtagtat
     2341 ctccaagtga tttacaaagt ttactctatg gccaccaggt aggggattcc ataacagtaa
     2401 ccttttatcg tggtgaaaat aaacaaacag tcactataaa acttactaaa actagtaaag
     2461 atttagctaa acaacgagca aataactaaa tcaattctag ttattttaac taagatatag
     2521 taaaattaaa ggggttggtc agatagacca atccctttag taagtctata gaatctgagg
     2581 tctaaaatgg aatatttaga aacgatcaac atcaatcata ttgctccaaa tccgtatcaa
     2641 cctcgcctag aatttaatac aaaagaacta gaagaattag ccaactctat taaaattaat
     2701 ggccttatcc aacccattat tgtaagaccg tcagcagtct ttggttacga actggtagcc
     2761 ggagaacgac ggctaagagc cgcaaagtta gctaaattgg aaagtatacc tgcaattata
     2821 aaatcctata ataatgatga tagtatgcaa ttagctattg tcgaaaattt acaacgatct
     2881 aatttgtcac ctattgaaga ggctaaagcc tactctcaac ttctacaaaa gaaaagtatg
     2941 acacatgaag agttagcaaa atatatggga aaatctcgtc cctatatatc aaatacgata
     3001 cgactactaa atttaccgcc actaattact tcagctattg aagaaggaaa actttcctct
     3061 ggtcatgcgc gtgcactact cagtctacca gatgctagtc aacagaaaga ttggtatcag
     3121 agaatcctca ctgaagatat tagtgtaaga cgactagaga agttattaaa acaagaaaag
     3181 aaaacaaatc ataagtcact tcaaaataaa gatgtctttc taaaacatca agaaaatgag
     3241 ttagctcaat ttctaggtag caaagtgaaa cttactatta ataaagatgg tgctggcaat
     3301 ataaagattg ctttcgctaa ccaagaagaa ttaaacagaa ttatcaacac tctaaaataa
     3361 gggtgttgat tattttttta tttaatcaaa cttatccaca aggtattttg ctatttttct
     3421 gttgattctc taatttccac agtctgtgga aaactttaat taacattgtt gattttattc
     3481 ttcaacatct gtggaaaact ttattttttt atggtacaat ataacaataa ttatccacaa
     3541 gacaataagg aagaagctat gacggaaaac gaacaactat tttggaatag agtactagag
     3601 ctatctcgtt ctcaaatagc accagcagct tatgaatttt ttgttctaga ggctagactc
     3661 ctcaaaattg aacatcaaac tgcagttatt actttagata acattgaaat gaaaaagcta
     3721 ttctgggaac aaaatttggg gcctgttatc ctaacagctg gttttgaaat tttcaatgct
     3781 gaaattacag ctaactatgt ctcaaacgat ttacatttac aagaaactag tttttctaac
     3841 taccagcaat ctagcaatga agtaaatact ttaccaatta gaaaaatcga ctctaacctt
     3901 aaagagaaat atacttttgc taattttgtt caaggagatg aaaatagatg ggctgtttca
     3961 gcatcaattg ctgtagctga tagtcctggc acgacttata atcctctatt tatctggggg
     4021 ggacctggtc taggaaagac gcatctacta aatgctattg gaaatcaagt cttaagagat
     4081 aatccaaacg cgagggtttt atacatcact gctgagaatt ttattaatga atttgtcagt
     4141 catattcgtt tagattcgat ggaagaatta aaagaaaagt ttcgcaactt agacttactc
     4201 ctgattgatg atattcagtc gcttgctaag aaaaccttag gggggaccca agaggagttc
     4261 ttcaatactt tcaatgcttt acatacaaac gataaacaaa tcgtattgac cagtgaccga
     4321 aatccaaatc aattaaatga tctagaagaa cgtctagtca cgcgctttag ttggggactc
     4381 ccagtaaata tcacaccacc tgattttgaa acacgagttg ctattttaac caataaaatt
     4441 caagaatatc cttatgattt tcctcaagat accattgaat acttagcagg agaatttgat
     4501 tccaacgtac gtgaattaga aggagccttg aaaaatatta gtctagttgc tgactttaag
     4561 catgctaaaa ctattacagt agatatagct gcagaagcta tcagagcacg taaaaatgat
     4621 ggtcctattg ttactgtcat tcctatagaa gaaattcaaa tacaagttgg taaattctat
     4681 ggcgtaactg taaaagagat aaaagcaact aaaagaacac aagatattgt ccttgcaaga
     4741 caggtagcca tgtacttagc tcgtgagatg acagataaca gtctcccaaa aataggtaaa
     4801 gaatttgggg gacgagatca ctcaactgtt ctccacgctt ataataaaat aaaaaatatg
     4861 gttgctcaag atgacaactt acgaattgag atagaaacta tcaaaaataa aatcagatag
     4921 gctttgtgga taagtatcag aaaaatacga gacttttcca caggttgtga acaagttatt
     4981 ttccttatga atattaaagt tataatactt attcacaact ttaacaggac
//
LOCUS       DBJYSH010000122         5015 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.122, whole genome shotgun sequence.
ACCESSION   DBJYSH010000122 DBJYSH010000000
VERSION     DBJYSH010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 5015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5015
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.122"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            171..395
                     /locus_tag="ACUBV3_001783"
     CDS             171..395
                     /locus_tag="ACUBV3_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin NrdH"
                     /protein_id="HHU3848264.1"
                     /translation="MAAITVFSKNNCMQCKMTKKFLDQHGADFEEINIDEKPEKIEYV
                     KNLGFSAAPVIEAGNVVFSGFQPSKLKELV"
     gene            473..2632
                     /gene="nrdE"
                     /locus_tag="ACUBV3_001784"
     CDS             473..2632
                     /gene="nrdE"
                     /locus_tag="ACUBV3_001784"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HHU3848265.1"
                     /translation="MSLKNIGDVSYFRLNNEINRPVNGQIPLHKDKEALTAFFKENVQ
                     PNSKAFDSITDKIAYLLKYDYLEEAFLNKYRPEFIEELSTKLFDKKFRFKSFMAAYKF
                     YQQYALKTNDGEYYLESIEDRVLFNALYFADGDEELATDLALEMISQRYQPATPSFLN
                     AGRSRRGELVSCFLIQVTDDMNAIGRSINSALQLSRIGGGVGISLSNLREAGAPIKGF
                     AGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLDVFHPDIISFLSTKKENADEKVR
                     VKTLSLGITVPDKFYELARNNQEMYLFSPYSIEREYGVPFSYIDITEKYDELVANPNI
                     TKTKINARDLETEISKLQQESGYPYIINIDTANRTNPVDGKIIMSNLCSEILQVQKPS
                     LINDAQEYLEMGTDISCNLGSTNVLNMMTSPDFGKSIKTMTRALTFVTDSSNIEAVPT
                     IKNGNAQAHTFGLGAMGLHSYLAKNHIEYGSPESIEFTDIYFMLMNYWTLVESNNIAR
                     ERQTTFVGFEKSKYADGTYFDKYVSGKFVPQSDKVKSLFTNHFIPEAKDWENLRYAVM
                     KDGLYHQNRLAVAPNGSISYINDCSASIHPITQRIEERQEKKIGKIYYPANGLATDTI
                     PYYTSAYDMDMRKVIDVYAAATEHVDQGLSMTLFLRSELPKELYEWKTESKQTTRDLS
                     ILRNYAFNKGVKSIYYIRTFTDDSSEVGANQCESCVI"
     gene            2835..3794
                     /gene="nrdF"
                     /locus_tag="ACUBV3_001785"
     CDS             2835..3794
                     /gene="nrdF"
                     /locus_tag="ACUBV3_001785"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HHU3848266.1"
                     /translation="MTTYYEAINWNEIEDVIDKSTWEKLTEQFWLDTRIPLSNDLDDW
                     RKLSAQEKDLVGKVFGGLTLLDTMQSETGVEAIRADVRTPHEEAVLNNIQFMESVHAK
                     SYSSIFSTLNTKSEIEEIFEWTNNNEFLQEKARIINDIYANGNALQKKVASTYLETFL
                     FYSGFFTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEDEQENFR
                     DWMYDLLYQLYENEEKYTKTLYDGVGWTEEVMTFLRYNANKALMNLGQDPLFPDTAND
                     VNPIVMNGISTGTSNHDFFSQVGNGYLLGSVEAMHDDDYNYGL"
     regulatory      3879..3977
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4034..4597
                     /gene="thiT"
                     /locus_tag="ACUBV3_001786"
     CDS             4034..4597
                     /gene="thiT"
                     /locus_tag="ACUBV3_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HHU3848267.1"
                     /translation="MSKNNNTTCLIETAIFAALAMALSMIPDFASWFTPSFGAIPLIL
                     FALRRGTKYGLFAGLIWGLLHFVLSKVYYLSLSQVFIEYILAFISMGLAGVFSAKFKD
                     ALSSSSKTKALSLALSGAILATFVRYVWHYIAGVIFWASYAPKGMSATLYSLSVNGTA
                     GLLTLFFVVISIIILVISYPSFFLPKK"
     gene            complement(4682..>5015)
                     /locus_tag="ACUBV3_001787"
     CDS             complement(4682..>5015)
                     /locus_tag="ACUBV3_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HHU3848268.1"
                     /translation="SLLFVLLGLHIYFLKVYRNIRGIKMTVAPTLKELKNNVLQLDEA
                     ELESNYKMCFDIVMNLSGFIFPTIYFVLFFISFVFQKVEIVAIIITTSIHIYILIKSL
                     KAARHFYR"
BASE COUNT         1679 a          876 c          822 g         1638 t
ORIGIN      
        1 aaaggaattg gaaaacaggc tgaaagcttt taaactaacc ctaaaaacga aatatagtgt
       61 ctcattttat tttaagacac tatatattgt gttttgttag aaataaaaca cttgcttttt
      121 ccattctttt tttatatgct attaccatga aaaaaaatta ggaggacaac atggcagcta
      181 ttactgtttt ttcgaaaaac aactgtatgc aatgtaaaat gactaagaaa tttcttgacc
      241 agcacggcgc tgattttgaa gaaattaata ttgatgaaaa accagaaaaa attgaatatg
      301 tgaaaaatct cggcttttct gcggctccag tgattgaagc tggtaatgtt gttttttcag
      361 gtttccaacc aagcaaacta aaagaacttg tttaagcaaa ttagagtctg ggaataaagc
      421 aattaattgc tttattccca ttttgtattg ttaagttaag aggataaatt caatgagttt
      481 aaaaaatatt ggcgatgtat cttactttcg cctaaataat gaaattaacc gtcctgttaa
      541 tggacaaatc ccactccata aagataaaga agccttaact gcttttttca aagaaaacgt
      601 acagcctaat tcaaaagctt ttgactcaat cactgataaa attgcttacc tccttaagta
      661 tgattatctt gaagaagctt tcctcaataa atatcgtcct gaatttattg aagaattatc
      721 taccaaactc ttcgataaaa agtttcgttt taaatccttc atggcagcct ataagtttta
      781 tcagcagtat gcattaaaaa caaatgatgg agaatactat ttagaaagta ttgaagatcg
      841 cgttctcttt aacgctcttt attttgcaga cggtgatgaa gaactagcaa ctgacttagc
      901 tctagaaatg atttcacaac gctatcaacc tgcaacacca agttttttaa atgctggtcg
      961 aagtcgtcgc ggagaattag tctcatgttt cttaatccaa gtaactgatg atatgaatgc
     1021 tattggtcgt tcgattaatt cagctcttca attatctcgt attggtggtg gggttggaat
     1081 atcacttagt aaccttcgtg aggctggagc tcctatcaaa ggttttgctg gtgctgcttc
     1141 tggagtcgtt ccagtcatga aattatttga agacagtttt tcttactcca accaacttgg
     1201 acaacgccaa ggtgctggag ttgtttacct tgacgttttc catccagata ttatttcatt
     1261 cttatcaact aaaaaagaaa atgctgatga aaaagtacgt gttaaaacat tgtcattagg
     1321 gattactgta cccgataaat tctatgaact agcacgtaac aatcaagaaa tgtacttgtt
     1381 tagtccttat agtatcgaac gtgaatatgg tgttcctttc agctatattg atattaccga
     1441 aaaatatgat gaattagtcg ctaaccctaa tatcactaaa acaaaaatca atgctcgtga
     1501 tttagaaaca gagatttcta aacttcaaca agaatctggt tatccttaca tcatcaatat
     1561 tgacactgct aatcgtacta atcctgttga cggaaaaatt attatgtcaa atctttgctc
     1621 tgagattttg caagtacaaa aaccaagtct tatcaatgat gctcaagaat accttgaaat
     1681 gggaactgat atttcttgta accttggctc aactaatgtt ttaaatatga tgacatctcc
     1741 tgattttgga aaatcaatta aaacaatgac tcgtgctcta actttcgtta ctgactcatc
     1801 aaatattgaa gctgttccaa ctattaagaa cggaaatgct caagcacata catttggact
     1861 tggagcaatg ggactccact cctatttggc aaaaaatcat atcgaatatg gtagtccaga
     1921 atcaattgaa tttactgata tttatttcat gctaatgaac tactggacct tagtagaatc
     1981 aaacaatatt gcccgcgaac gtcaaacaac atttgtaggt tttgaaaaat caaaatacgc
     2041 tgacggtaca tactttgata aatatgtatc tggaaaattt gttccacaat ctgacaaagt
     2101 aaaatcccta tttactaatc attttatccc cgaagctaaa gattgggaaa accttagata
     2161 cgcagttatg aaagacggac tatatcatca aaatcggctt gcagttgctc cgaatggttc
     2221 tatttcgtat atcaacgatt gttcagcatc tatccaccct attacacaac gtattgaaga
     2281 gcgtcaagag aaaaaaatcg ggaaaattta ttatccagct aatggcttag ctacagatac
     2341 tatcccttat tacacttctg cctatgatat ggatatgcgt aaagtaatag acgtctatgc
     2401 cgcagcaact gagcacgtag accaaggtct ctcaatgaca ctctttttac gaagcgaatt
     2461 accaaaggag ttgtacgagt ggaaaacaga aagtaagcag actactcgcg acctttcaat
     2521 cctacgtaac tatgctttca acaaaggtgt gaaatcaatc tactatatcc gtacttttac
     2581 agatgatagt agcgaagtcg gagctaatca atgcgaaagt tgtgtcatct aacgaagaaa
     2641 ctctacttaa aacatcagaa actagactga gataaactat ctcagtttca tgatgttatc
     2701 ttattttcta aggtattttc atctttattt ttctaaaaat cgataatact tgaaatattg
     2761 ctagatcaga attctatatt ctactcttct tccatcatca ttatataaaa acctatttac
     2821 cattggagaa aaccatgaca acttactacg aagctataaa ctggaacgaa attgaagatg
     2881 ttattgataa atcaacttgg gaaaaactaa ccgaacaatt ttggctcgat acacgtatcc
     2941 ctttatcaaa tgacttagac gattggcgca aactttccgc tcaagaaaaa gatcttgttg
     3001 gcaaggtttt tggaggctta accctacttg ataccatgca atcagaaact ggtgttgaag
     3061 ctattcgtgc cgatgttcgc acgcctcacg aagaagctgt cttaaacaat attcaattca
     3121 tggaatctgt tcacgctaaa tcttattctt caattttctc aactttaaat actaaatcag
     3181 aaattgaaga aattttcgag tggactaata ataatgagtt ccttcaagaa aaagcacgta
     3241 ttatcaatga catttatgct aatggaaatg cccttcaaaa aaaggtggct tccacctacc
     3301 tcgaaacttt ccttttttat tctggctttt tcacacctct ttactatttg ggaaataata
     3361 agttagcaaa tgttgctgaa atcattaaat taattattcg tgatgaatct gtacatggta
     3421 cttatatcgg ttacaaattc cagcttggtt ttaacgaatt accagaagat gagcaagaga
     3481 attttcgtga ttggatgtat gacctccttt atcagctgta tgaaaacgaa gaaaaataca
     3541 ccaagacact ttatgatggc gtaggatgga ctgaagaagt tatgaccttt ttacgctaca
     3601 atgctaataa agctcttatg aatttaggac aagatccttt attcccagat acagcaaatg
     3661 atgtcaaccc aattgttatg aatggtattt caacaggaac atcaaaccat gacttcttct
     3721 ctcaagtagg taatggttac ctacttggta gcgttgaagc tatgcatgat gatgactata
     3781 actatggatt ataagaaaaa gacactgatt tagtgtcttt ttctagttaa atacaaattt
     3841 cttgacaagc aaaccctttc aagatagaat gttagagtaa atacacaagg gagtgccttg
     3901 agctgagatt gcagatatgc aaaatcctct aacctgatct cgttaggacg agcgtaggaa
     3961 ttgtggtttg aattggttaa acatgacaaa tgacaaaaga atctcctttg ctgtataaaa
     4021 aaggagattt tttatgtcaa aaaataacaa cactacctgt cttattgaaa ctgctatttt
     4081 tgcagcgctt gctatggctt tatcaatgat tcctgatttt gcgtcatggt tcactccctc
     4141 ttttggagca attcctctga tactttttgc attacgtcgt ggtacaaaat acggtctatt
     4201 tgctggctta atttggggct tattgcactt tgttctttcc aaagtatatt accttagttt
     4261 atcccaagtt tttatagaat acatcttagc atttataagt atgggacttg caggcgtatt
     4321 ttctgctaaa tttaaagatg cacttagcag cagctctaaa actaaagctt tatcattagc
     4381 attatctgga gcaatattag caacatttgt tcgttatgtt tggcattata tcgctggtgt
     4441 tattttttgg gctagttatg caccaaaagg tatgtctgct acattatact cattaagcgt
     4501 caatggtact gctggtttgt taactctatt ttttgtagtc attagtatta ttattttagt
     4561 tatcagctac cctagcttct ttttacctaa gaaatagaaa aaataaaagg actgattttc
     4621 agagaatcaa tccttttatt ttacactaat ataagttttt tgataaaaaa tctagctact
     4681 actatctata aaaatgacgt gctgctttta aagacttaat gagaatgtag atatgaatag
     4741 atgttgttat aataattgct acaatctcca ctttttgaaa aacaaatgag ataaagaata
     4801 atacaaaata gatcgtaggg aaaataaaac cacttaaatt catgacaata tcaaaacaca
     4861 tcttataatt tgattccaac tctgcttcgt ccaattgtaa gacattgttt ttcaactctt
     4921 ttaaagttgg agcgactgtc atttttatac ctcggatatt ccgatatacc tttaaaaaat
     4981 atatatgtaa acccaataaa acaaataaca gtgat
//
LOCUS       DBJYSH010000123         4998 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.123, whole genome shotgun sequence.
ACCESSION   DBJYSH010000123 DBJYSH010000000
VERSION     DBJYSH010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 4998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4998
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.123"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            14..84
                     /locus_tag="ACUBV3_001788"
     tRNA            14..84
                     /locus_tag="ACUBV3_001788"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:46..48,aa:Gly,seq:tcc)
     gene            119..192
                     /locus_tag="ACUBV3_001789"
     tRNA            119..192
                     /locus_tag="ACUBV3_001789"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:153..155,aa:Ile,seq:gat)
     gene            206..293
                     /locus_tag="ACUBV3_001790"
     tRNA            206..293
                     /locus_tag="ACUBV3_001790"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:240..242,aa:Ser,seq:gct)
     gene            529..1872
                     /gene="pcsB"
                     /locus_tag="ACUBV3_001791"
     CDS             529..1872
                     /gene="pcsB"
                     /locus_tag="ACUBV3_001791"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan hydrolase PcsB"
                     /protein_id="HHU3848269.1"
                     /translation="MKKRILSAVLVSGVTLGTAAVTVNADDFDSKIAATDSVINTLSG
                     QQAAAQNQVTAIKGQVGALESQQSELEAQNAQLEAVSQQLGQEIQTLSNKIVARNESL
                     KKQVRSAQKGNLTNYINTILNSKSVSDAVNRVVAIREVVSANEKMLAQQEADKAALEA
                     KQIENQNAINTVAANKQAIENNKAALATQRAQLEAAQLELSAQLTTVQNEKASLIQAK
                     AQAEEAARKAAEAQAAAEAKAQAEAKAQAESVAKAQAAAQVESATAPTETVQTQPRTE
                     IKPSNLTATSSATTVATTTATATNEPKVTQPSVVTKAVEAPKAVVSSTPRAVSKPVVR
                     SYDSSNTYPMGQCTWGAKSMASWVGNYWGNANQWGASARAAGYSVGTTPRVGAVAVWP
                     YDGGGYGHVAVVTSVANNSSIQVMESNYAGNMSIGNYRGSFNPSASGSVYYIYPN"
     gene            1996..2964
                     /locus_tag="ACUBV3_001792"
     CDS             1996..2964
                     /locus_tag="ACUBV3_001792"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000122447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HHU3848270.1"
                     /translation="MSYSNLKLFALSSNKELAKKVSQTIGIPLGQSTVRQFSDGEIQV
                     NIEESIRGHHVFILQSTSSPVNDNLMEILIMVDALKRASAESVSVVMPYYGYARQDRK
                     ARSREPITSKLVANMLEVAGVDRLLTVDLHAAQIQGFFDIPVDHLMGAPLIADYFDRQ
                     GLVGDDVVVVSPDHGGVTRARKLAQCLKTPIAIIDKRRSVTKMNTSEVMNIIGNIKGK
                     KCILIDDMIDTAGTICHAADALAEAGATAVYASCTHPVLSGPALDNIQNSAIEKLIVL
                     DTIYLPEERLIDKIEQISIAELIGEAIIRIHEKRPLSPLFEMNKLK"
     gene            3072..4247
                     /locus_tag="ACUBV3_001793"
     CDS             3072..4247
                     /locus_tag="ACUBV3_001793"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HHU3848271.1"
                     /translation="MTLEKRFNKYLDRIEVSLIRQFDQSISDIPGMVKLTLGEPDFTT
                     PDHVKEAAKSAIDANQSYYTGMSGLLALRQAAADFAKDKYNLTYNPDCEILVTIGATE
                     ALSASLIAILEAGDVVLLPAPAYPGYEPIVNLVGADIVEIDTRENDFRLTPEMLETAI
                     IQQGEKLKAVLLNYPTNPTGITYSRQEIAALAEVLKKYDIFVISDEVYSELTYTGQQH
                     VSIAEYLPNQTILINGLSKSHAMTGWRVGLVYAPEAFIAQIIKSHQYMVTAASTISQF
                     AGVEALSVGKNDTLPMRQGYIKRRDYIIDKMSKLGFKIIKPSGAFYIFAKIPDSYPQD
                     SFKFCQDFAYQQAVAIIPGVAFGKYGEGYIRLSYAASMEVIETAMARLKVFMESYEG"
     gene            4237..4998
                     /gene="recO"
                     /locus_tag="ACUBV3_001794"
     CDS             4237..4998
                     /gene="recO"
                     /locus_tag="ACUBV3_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HHU3848272.1"
                     /translation="MRVSQTYGLVLYNRNYREDDKLVKIFTETEGKRMFFVKHASKSK
                     FNAVLQPLTIAHFILKINDNGLSYIDDYKEVLAFQETNSDLFKLSYASYITSLADVAI
                     SDNVADAQLFIFLKKTLELIEDGLDYEILTNIFEVQLLERFGVALNFHDCVFCHRVGL
                     PFDFSHKYSGLLCPNHYYKDERRNHLDPNMLYLINRFQSIQFDDLQTISVKPEMKLKI
                     RQFLDMIYDEYVGIHLKSKKFIDDLSSWGSIMKSD"
BASE COUNT         1572 a          797 c         1008 g         1621 t
ORIGIN      
        1 atataagttt ttagcgggtg tagtttagtg gtaaaactac agccttccaa gctgttgtcg
       61 cgagttcgat tctcgtcacc cgcttacttt acataaaagt aaaaccaagc atttgcttgg
      121 gcgcgtagct caggtggtta gagcgcacgc ctgataagcg tgaggtcggt ggttcgagtc
      181 cactcgtgcc cattaatatt aatttggaga attactcaag aggctgaaga ggacggtttg
      241 ctaaatcgtt aggtcgggta actggcgcaa gggttcgaat cccttattct ccgtaaatat
      301 tataatttta agagtaatta tactcttttt ttatttctct tttagtttca ctatggagta
      361 attatattga tagagtgaat agaagaaggt tatcgttttc tagatttgat tatggttcat
      421 ttagaaatag ttgatttcaa atataaatgt aacaatgacg taatataatt tcgtgaattt
      481 tttggtaaaa tatttaatgt cttattgtaa aggagtagtt taataaaaat gaaaaaaaga
      541 atattatcag cagtccttgt gagtggagta actcttggaa ctgctgccgt aacagtaaac
      601 gctgatgact ttgactcgaa aattgctgct actgattcag taatcaatac tttatcaggg
      661 caacaagcag cagcacaaaa ccaggtgact gctattaaag gacaagttgg tgctttagag
      721 tctcaacaat cagaattaga agcgcaaaat gctcaacttg aggctgtatc tcagcagtta
      781 ggtcaagaga ttcaaactct ttctaataaa attgttgcac gtaacgaatc tttgaaaaag
      841 caagttcgta gtgctcaaaa gggtaacttg actaactata ttaatactat tttaaattcg
      901 aaatcagtat ctgatgctgt taatcgtgtt gttgctattc gtgaggttgt ttcagctaat
      961 gaaaagatgt tagcacaaca agaggctgac aaagcggcct tagaagctaa acaaattgaa
     1021 aatcaaaatg ctataaatac tgtagcagct aacaagcaag ctatcgaaaa taataaagca
     1081 gccttagcaa cacaaagagc acaattagaa gcagctcaat tagagttatc tgctcaattg
     1141 actacagtcc aaaatgaaaa agcatcttta attcaggcaa aagcacaagc tgaggaagct
     1201 gcgcgtaagg cagctgaagc acaagcagca gctgaagcga aagcacaagc tgaagcgaaa
     1261 gcacaagctg agtctgtagc aaaagcacag gcagcagcac aagttgagtc tgccacagct
     1321 cctactgaaa cagttcaaac acaaccgaga actgaaataa agccttctaa tcttacggct
     1381 acttcttcgg caactactgt tgctacaact acagctacag ctactaatga gccaaaagtt
     1441 actcaacctt ctgtagtaac taaagcagtt gaagcaccta aagcagttgt ttcttcaaca
     1501 cctagagcgg tatcaaaacc tgttgtcaga agttatgatt caagtaacac atacccaatg
     1561 ggacaatgta cttggggagc taagtcaatg gcttcttggg ttggtaacta ttggggaaat
     1621 gctaatcagt ggggtgctag tgcacgtgca gctggttatt ctgtaggaac tacccctcgt
     1681 gttggtgcag tagctgtttg gccatatgat ggtggtggtt acgggcacgt tgcagtagta
     1741 acgtcagtgg ctaacaattc atctatccaa gttatggaat caaactatgc tggaaatatg
     1801 agtattggca attaccgtgg ttcatttaac cctagtgctt ctggctctgt ttactatatt
     1861 tacccaaatt aatgaatact aaggagatct ttcgtggtct cctttttgtt tattaattag
     1921 gtatggttta ttttggattg aaaaattgtg taaaaagcgt taaaatggta taggactaaa
     1981 gttttggagg aaatcatgtc ttattctaat ttaaaattgt ttgcactgtc atcaaacaaa
     2041 gaactagcga aaaaagtgtc tcagacgatt ggaattccgc taggtcaatc gacagttcgc
     2101 caattttctg atggtgagat tcaagttaat attgaagagt ctattcgtgg ccatcatgtt
     2161 tttattttac aatcaacaag ctcaccagtt aatgataatt tgatggaaat tttaattatg
     2221 gttgacgctt taaagcgtgc tagtgcggag tctgttagtg tggtaatgcc ttactatggg
     2281 tatgctcgtc aagatcgcaa ggcacgttct cgtgaaccta ttacttcgaa actcgttgcg
     2341 aacatgctag aagttgctgg tgttgatcgt cttttaacag ttgatttaca tgcagctcaa
     2401 attcagggat tctttgatat tcccgttgat caccttatgg gggctcccct tattgcagat
     2461 tactttgatc gtcagggatt ggttggtgat gatgttgttg tcgttagtcc tgatcatggt
     2521 ggcgtaacac gtgcacgtaa gctggcacag tgtttaaaga caccgattgc tatcattgat
     2581 aagcgccgta gtgttactaa aatgaataca tctgaagtaa tgaatattat tggtaatatc
     2641 aaaggtaaaa aatgtatttt aattgatgat atgattgata ctgcaggtac tatttgccat
     2701 gctgcagacg ctttagctga agcaggggca acagcggttt atgcttcatg tactcaccct
     2761 gttttatcag ggccggcttt ggataatatt caaaattcag ctattgaaaa attaattgtg
     2821 ttagatacga tttacctccc tgaagaacgt ttgattgata agattgaaca aatttcaatt
     2881 gcggaattga ttggtgaagc tattatccgt attcacgaga aacgtccttt atcaccatta
     2941 tttgaaatga ataaactaaa atgacaaaaa acctgttatt aacaggtttt ttatcattta
     3001 atgacggaaa actggacaca aattattttt cttgtataat taaatatatt atttcttatc
     3061 aggaggttat gatgacatta gagaaacgat ttaataagta cttagataga atagaggtct
     3121 ccttaattcg tcagtttgat caatctattt cagatatacc tggaatggta aaattaactc
     3181 ttggagaacc agattttacc actcctgatc atgttaaaga agcagctaaa tcagctattg
     3241 atgcgaatca atcgtattac actggtatga gcggtttatt agctctaagg caagctgctg
     3301 cagattttgc aaaggataaa tataatctta cttataatcc tgattgtgag atcttggtta
     3361 cgataggtgc cacagaagct ctgtctgcat ctcttatagc tattttagaa gctggtgatg
     3421 ttgttctatt acctgccccc gcttatccag gatatgagcc aatagttaac cttgtaggag
     3481 ctgatatcgt agaaattgat actagagaaa atgactttag actaactcct gaaatgttag
     3541 aaacggctat tatccaacaa ggtgaaaaat taaaggctgt tttacttaat tatcctacta
     3601 accctacagg tattacctat tcaaggcaag aaattgctgc attagctgaa gtccttaaaa
     3661 aatatgatat ttttgttatt agtgatgaag tttattctga gttaacttac actggtcagc
     3721 agcatgtttc aattgcagaa tatttaccta accaaactat cttaattaat ggcttatcaa
     3781 aatcacatgc tatgactggt tggagagttg gattggtgta tgctcctgaa gcatttattg
     3841 ctcaaatcat taaaagtcat caatacatgg tgacagctgc tagtactatt tcgcaatttg
     3901 ctggggtaga agccttatct gtgggtaaaa atgatacgtt acctatgcgt caaggatata
     3961 ttaaacgtag agattatatc attgataaaa tgtctaaact tggtttcaaa ataattaaac
     4021 cgagtggcgc tttttatatc tttgctaaaa tacctgatag ctatccacag gattctttca
     4081 agttttgtca ggattttgct tatcaacaag ctgtagctat tattccagga gtggcttttg
     4141 gtaaatatgg tgaaggatat atacgtcttt cttatgctgc tagtatggaa gttattgaaa
     4201 cagcaatggc tagattgaaa gtttttatgg aatcatatga gggttagtca aacatacggt
     4261 ctcgttttgt ataatcgtaa ttatcgtgaa gatgataaat tagttaaaat ctttacggag
     4321 actgagggga aaaggatgtt ttttgtaaag catgcttcaa agtcaaaatt caatgctgtg
     4381 ctccaacctt tgactattgc tcattttatt ttaaaaatta atgataatgg tctttcttat
     4441 attgatgatt ataaagaagt tttagcattt caagaaacca attcagactt gtttaagttg
     4501 tcatatgcga gttatattac ttctttggct gatgtggcta ttagtgataa tgtagcggat
     4561 gctcaattat ttattttcct aaagaaaacg ttagaattga ttgaggacgg tttagattat
     4621 gagattctaa cgaatatttt tgaagtacaa ctactagaga ggttcggtgt tgctttaaat
     4681 tttcatgatt gtgttttttg tcatagggta ggattacctt ttgatttttc acacaaatat
     4741 tctggattat tatgtccaaa ccattattat aaagacgaga gaagaaacca cctagatcct
     4801 aatatgctgt acttaatcaa tcgttttcag tcaattcaat ttgatgattt acaaacaatt
     4861 tctgtgaaac ctgagatgaa acttaaaatt cgtcaatttt tggacatgat ttacgatgaa
     4921 tatgtaggga ttcatcttaa aagtaaaaaa tttattgatg atttgtctag ttggggaagt
     4981 attatgaaat cagattaa
//
LOCUS       DBJYSH010000124         4769 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.124, whole genome shotgun sequence.
ACCESSION   DBJYSH010000124 DBJYSH010000000
VERSION     DBJYSH010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 4769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4769
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.124"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            40..1197
                     /locus_tag="ACUBV3_001795"
     CDS             40..1197
                     /locus_tag="ACUBV3_001795"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HHU3848273.1"
                     /translation="MNKLKVNSVVERKIKSGAQLLEKKDFDTSLVNQLVQLFSQSNQF
                     LGMAYLSPQNKGIGWLLSRQIFDFNHDYFVSLFEKSREKRQKFEKSSQTTAYRLFNQD
                     GDNFGGLTIDFYSDYALFSWYNEFVYTNRQMIVAAFKQVYPNIKGAYEKIRFKGLDFE
                     SAHLYGQEAPESFLILENNIKYSVFLNDGLMTGIFLDQHDVRKALATNLSEGKKVLNM
                     FSYTAAFSVAAAVGGALETTSVDLAKRSRELSKAHFDANQIVTDNHRFIVMDVFEYYK
                     YAKRKHLSYDVIVIDPPSFARNKKQTFSVTKDYYKLIEQALDILAPGGTIIASTNAAN
                     LTVSQFKKQLEKGFGKASHNYISLQQLPEDFTVNDKDQQSNYLKVFTIKVK"
     gene            1197..1874
                     /gene="aroD"
                     /locus_tag="ACUBV3_001796"
     CDS             1197..1874
                     /gene="aroD"
                     /locus_tag="ACUBV3_001796"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HHU3848274.1"
                     /translation="MKIVVPVMPRSLEEAQEIDLSKFDSVDIIEWRADALPKDDIINV
                     APAIFEKFAGHEIIFTLRTTREGGNIVLSDAEYVELIQKINSIYNPDYIDFEYFSHKE
                     VFQEMLEFPNLVLSYHNFQETPENIMEIFSELTALAPRVVKIAVMPKNEQDVLDVMNY
                     TRGFKTINPDQVYATVSMSKIGRISRFAGDVTGSSWTFAYLDSSIAPGQITISEMKRV
                     KALLDAD"
     gene            1968..3035
                     /gene="aroB"
                     /locus_tag="ACUBV3_001797"
     CDS             1968..3035
                     /gene="aroB"
                     /locus_tag="ACUBV3_001797"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001195728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HHU3848275.1"
                     /translation="MQVEVDLPNHPYHIKIEEGCFSEAGDWVSHLWQKQMITIITDSN
                     VEILYGESLVNQLKKQGFTVHVFSFAAGEASKTLEVANRIYGFLAKHHMTRSDGIIAL
                     GGGVVGDLAAFVASTYMRGIHFLQIPTSLTAQVDSSIGGKTGVNTSFAKNMVGTFAQP
                     DGVLIDPVTLKTLGNRELVEGMGEVIKYGLIDDIKLWHILEEMDGSIDSILDNALAII
                     YHSCQVKRKHVLADQYDKGLRMHLNFGHTIGHAIEVHAGYGEIMHGEAVAIGMIQLSR
                     VAERKNLMPRGISQDIYNMCLKFGLPVHYAEWDKDVLFNILSHDKKASGQFIKIVILP
                     QLGSATVHQIPLEEMRDYLEK"
     gene            3036..4202
                     /gene="aroC"
                     /locus_tag="ACUBV3_001798"
     CDS             3036..4202
                     /gene="aroC"
                     /locus_tag="ACUBV3_001798"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HHU3848276.1"
                     /translation="MRYLTAGESHGPSLTAIIEGIPAGLKLSAKDINEDLKRRQGGYG
                     RGNRMKIETDQVIISSGVRHGKTLGSPITLTVTNKDHSKWLDIMSVEDIEERLKQKRR
                     IKHPRPGHADLVGGIKYRFDDLRNALERSSARETTMRVAIGAIAKRILKEIGIEIANH
                     IVVFGGKEITVPDKLTVQQIKVLSSQSQVAIVNPSFEQEIKDYIDSVKKAGDTIGGVI
                     ETIVGGVPVGLGSYVHWDRKLDAKIAQAVVSINAFKGVEFGLGFKSGFLKGSQVMDSI
                     SWTKDQGYIRQSNNLGGFEGGMTNGEPIVVRGVMKPIPTLYKPLMSVDIDTHEPYRAT
                     VERSDPTALPAAGVVMEAVVATVLVTEVLEKFSSDNMYELKEAVKLYRNYVNHF"
     gene            4286..4621
                     /locus_tag="ACUBV3_001799"
     CDS             4286..4621
                     /locus_tag="ACUBV3_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HHU3848277.1"
                     /translation="MANVYDLANELERAVRALPEYQAVLTAKAAIENDADAQVLWQDF
                     LATQSKVQEMMQSGQMPSQEEQDEMSKLGEKIESNDLLKVYFDQQQRLSVYMSDIEKI
                     VFAPMQDLM"
BASE COUNT         1569 a          737 c          970 g         1493 t
ORIGIN      
        1 cagtatgaaa aaagataaaa atttgttata atactgttta tgaataaact taaagtgaat
       61 tctgtcgttg aaagaaaaat caaatcaggt gctcagttac tggaaaaaaa agattttgat
      121 accagtttag ttaaccagtt ggttcaactt ttttcacagt caaatcaatt cttagggatg
      181 gcctatcttt caccacaaaa taaagggatt ggttggttac tatcaagaca aatttttgat
      241 tttaaccatg attactttgt atcgctattc gaaaaatcta gagagaagcg tcaaaaattt
      301 gaaaaatcta gccaaacaac agcctaccgc ttgtttaatc aggatggaga taactttggt
      361 ggcctaacta tagattttta tagtgactat gctctttttt cgtggtacaa tgaatttgtt
      421 tatactaatc gacaaatgat tgttgcagcc tttaagcagg tctatcctaa tattaaaggg
      481 gcatatgaaa aaattcgttt caaaggttta gactttgaaa gtgctcattt gtacggtcaa
      541 gaggctcctg aatcattttt gattttagaa aataatatca aatatagtgt ctttttgaat
      601 gatgggttaa tgacaggtat tttccttgac caacatgatg tcagaaaagc cttagcaact
      661 aatctatcag aaggtaaaaa agttctaaat atgttttcat acactgcggc tttttctgta
      721 gctgcagctg ttggaggagc attagagaca acttctgttg atttagcaaa acgctcgcgt
      781 gaactttcaa aagcacactt tgatgctaat cagattgtca cagataacca tcgatttatc
      841 gttatggatg tttttgaata ttataagtat gccaaaagaa aacatctatc atatgatgtg
      901 attgttattg atccaccaag ttttgctcga aataaaaaac aaactttttc ggttactaaa
      961 gattactata aattaattga acaagcttta gatattttag cccctggtgg aactatcatt
     1021 gcatcaacta acgcagctaa tctaaccgta tcacaattta agaaacaatt ggaaaagggg
     1081 tttggtaaag cttcacataa ttacattagc ttacagcagt tgcctgaaga tttcactgta
     1141 aatgataagg accaacaaag taattattta aaagtattta caataaaggt aaaataatga
     1201 aaatagtagt accagtaatg cctcgcagtc ttgaagaggc tcaagaaata gatttatcaa
     1261 aatttgatag tgttgatatt attgaatggc gagctgatgc cttaccaaag gatgacatta
     1321 ttaatgtagc tccagctatt tttgagaaat tcgcaggtca tgaaattatt tttactcttc
     1381 gtacaacgcg tgaaggtggt aatattgtct tatctgatgc tgagtatgtt gagttaatcc
     1441 agaaaattaa ttctatctac aatccagatt atattgattt tgagtatttt tcacataaag
     1501 aagtttttca agaaatgcta gaatttccaa atttagtcct gtcttatcac aattttcaag
     1561 agacaccgga gaatattatg gagatatttt cagaattaac agccctagca ccacgagttg
     1621 tgaaaatcgc agtaatgcca aagaatgaac aagatgtctt agacgttatg aattacactc
     1681 gcggtttcaa gactattaat cctgatcaag tttatgcgac ggtatctatg agtaaaattg
     1741 gacgtatttc tcgttttgct ggtgatgtaa ctggatctag ttggacattt gcatatttag
     1801 attcatctat cgcacccgga caaattacta tttcagagat gaagcgtgtc aaagcattgc
     1861 ttgacgctga ctgatacgag ataacaaaat gttaaatata ctgatgagat ccctagcaat
     1921 tgcttagggt ttttgtctta ttaaaacaaa catgagagga cattagaatg caggttgaag
     1981 ttgacttacc gaatcaccct tatcatatta aaattgaaga aggatgtttt tctgaggctg
     2041 gtgactgggt atcacattta tggcagaaac aaatgataac tattattaca gatagtaatg
     2101 tagaaatatt atacggggag tcgcttgtca accagttaaa aaaacaaggt tttacagtac
     2161 atgtttttag ttttgctgcg ggtgaagcta gtaaaacttt ggaagttgct aatcgtattt
     2221 atggtttttt agcaaagcat catatgacca gaagtgatgg gatcattgca cttggtggag
     2281 gcgtcgtcgg agatttagca gcgtttgttg cttctactta tatgcgggga atccattttc
     2341 ttcaaattcc aactagttta acagcacagg ttgattcttc cattggcgga aaaacaggag
     2401 tcaacacttc attcgctaaa aatatggtag gtacttttgc acaacctgat ggtgtactga
     2461 tagatcctgt tactctaaaa acattgggca atcgtgaatt agttgaaggt atgggagaag
     2521 tcatcaaata tggcttgatt gatgatataa aactctggca tatcttagaa gagatggatg
     2581 ggtctattga tagtatcctt gataatgcat tagctattat ttatcactct tgtcaagtta
     2641 aacgaaagca tgttttagct gatcaatatg ataaagggtt aaggatgcat ttaaattttg
     2701 gccacactat cggtcatgct atcgaagtac atgctggcta tggagaaata atgcatggag
     2761 aagctgttgc aattggaatg atccagcttt ctcgtgtggc tgagcgtaaa aatcttatgc
     2821 caagaggtat cagtcaagat atttataata tgtgtttaaa atttggcctt ccggtccact
     2881 atgcagaatg ggataaggac gtcttattta acatattgag tcatgataaa aaagcaagtg
     2941 gacaatttat taaaattgtt atactgcctc aattaggtag tgcaacagtc caccaaatac
     3001 ctttagaaga aatgagagat tatttggaga aatagatgag atatttaact gctggtgagt
     3061 cacatggacc tagtttaaca gctattatcg aagggattcc tgcgggactg aaattatctg
     3121 ctaaagatat taatgaagat ttaaaacgcc gccaaggtgg ttatggtcgt gggaatcgaa
     3181 tgaaaattga aactgatcaa gtgatcatta gttctggtgt gcgccatgga aaaaccttgg
     3241 gttcacctat tacactgaca gtaactaata aggatcatag caaatggctg gacatcatgt
     3301 cagtcgaaga cattgaagag cgtctaaagc aaaaacgtcg tattaagcat cctagacctg
     3361 ggcacgcaga tttagtaggt ggaatcaaat accgatttga tgatttgaga aatgctttag
     3421 agcgttcttc ggctcgtgag accacaatgc gagtagctat tggtgcgatt gctaaacgta
     3481 tcttgaaaga aattggtatc gaaattgcta accatattgt agttttcggt ggcaaggaga
     3541 ttactgtccc tgataagcta acagttcaac aaatcaaagt gttatctagt caatcgcaag
     3601 tagctatcgt caatccatca tttgaacaag aaattaagga ttatattgac agtgtgaaaa
     3661 aggctggaga tactatcgga ggagtgattg aaactatcgt tggaggagtt cccgttggtt
     3721 taggctctta tgtacactgg gatagaaagt tagatgctaa gatagcgcaa gcagttgtat
     3781 ctatcaatgc ttttaaaggt gttgaatttg gtctaggttt taagtcaggg tttttaaaag
     3841 gaagtcaagt aatggactcc atatcatgga caaaggacca aggttacata cgtcagtcaa
     3901 ataatttagg cggttttgaa ggcggtatga ctaatggaga gcctattgtc gttaggggag
     3961 taatgaagcc aattcctacg ctttacaagc cactaatgtc agttgatatt gatactcacg
     4021 agccttatag agctacagtg gaacgttctg atcccacggc acttccggct gcaggtgtcg
     4081 ttatggaagc tgtagtagcc accgtcctgg tgacagaagt gctagaaaaa ttttcttcag
     4141 acaacatgta tgaactaaaa gaagctgtaa aactatatcg caattatgtt aatcattttt
     4201 aagtgaaaac tagtagcatt caattgcaaa aaaaggtata aaagtctaca atagtggtaa
     4261 gttcaagtag aaaaaggagt aacttatggc taatgtatat gatttagcaa atgaattaga
     4321 acgtgctgtt cgtgctttac cagaatacca agcagtttta actgcaaaag cagctattga
     4381 aaatgatgcg gatgcacaag tgctttggca agactttttg gctacccaat caaaagttca
     4441 agaaatgatg caatctggcc aaatgccaag tcaagaagaa caagatgaaa tgtctaaact
     4501 tggggaaaaa attgaatcca atgacctttt aaaagtttat tttgaccaac aacaacggtt
     4561 gtctgtctat atgtctgata tcgaaaaaat tgtctttgca cccatgcagg acttgatgta
     4621 atagtataaa agagagagct ccttgagttt tctcttttat gctatttaca ttcagagcat
     4681 cctaattgct gcgcttttac attattgcat tatcctataa aaatgtattt ttatactttt
     4741 tttgagagcg gctatttatt tttagactt
//
LOCUS       DBJYSH010000125         4499 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.125, whole genome shotgun sequence.
ACCESSION   DBJYSH010000125 DBJYSH010000000
VERSION     DBJYSH010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 4499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4499
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.125"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            complement(<1..974)
                     /locus_tag="ACUBV3_001800"
     CDS             complement(<1..974)
                     /locus_tag="ACUBV3_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HHU3848278.1"
                     /translation="MSDVVEKQTTKSFIMNVLNGLALGTVIVLIPGAILGELMKALLP
                     MWSGFATLIAATAVATSMMGLVIGIMVGLNFKFNPIQSASLGLAVMFAGGAATFLKGA
                     IMLKGTGDIINMGITAALGVLLIQFLSDKTKSFTLIVIPTVTLLLVGGVGHVLLPYVK
                     MITTMIGQGIASLLGLQPVLMSILIAMIFCFLIVSPITTVGIALAISLSGIGSGAANL
                     GICAASFGLCMAGWSVNSKGTALAHVLGSPKISMANVLAKPKIMLPMISSAAILGILG
                     ALFNIQGTPASAGFGISGLIGPINALNLAKGGWSVMNMLLIIIIFVAAP"
     gene            1167..2051
                     /locus_tag="ACUBV3_001801"
     CDS             1167..2051
                     /locus_tag="ACUBV3_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HHU3848279.1"
                     /translation="MDIRQLTYFIAVAEAKNYSRAAKSLFVTQPTLSQSIKKLEAELN
                     TILFLQNGRQLALTEAGEILYEKGQLLMTNVNQMVTEIQQLNQEKKEGIRVGLTSLFA
                     IQFMKQISTFMATHSNVEVSLIQDGSRKLQELLAKGKIDIGLLSFPSTRNDITIEPLQ
                     TSTKGYKVSIVMPKSHPLATLPEIELNDLRDYKVASLNEHYMLGEMLPRKCRALGFDP
                     HIVFKHNDWEVLIHSLQDLNAVTILPSEFESISQVQDLCWVPLKDKNNFYPIGIAYRN
                     DTSFSPMIEEFLSLLKTN"
     gene            complement(2088..2906)
                     /locus_tag="ACUBV3_001802"
     CDS             complement(2088..2906)
                     /locus_tag="ACUBV3_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATPase"
                     /protein_id="HHU3848280.1"
                     /translation="MILKICRAAYSLQWGGVYQLALLDYPRIKAFELERIGAFIAYEK
                     QYKRKTEIQCDDKHLLAKIVHFLKYNSFTFPYIPKYREAAATFNEDGISLTSDFLSHT
                     CTIETAKLIFKEGKILSAVKAFNKPAEVLVKDKRNAAGDPKDYFDYVMLNWSNTNSGY
                     RLVMERLLGKAPSEQELTVGFKPGVSFHFTYQDIINHPDSIFDGYHPAKIKNQLSLAE
                     HLVACVIPKHYQEDYQSLVPNDLKHRVYYLDYCNETLYEWNQKVYDFLCHLENK"
     gene            complement(3070..3843)
                     /locus_tag="ACUBV3_001803"
     CDS             complement(3070..3843)
                     /locus_tag="ACUBV3_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HHU3848281.1"
                     /translation="MSGANSISLMSLLMASSLVLITLFFSYWQKLNLEKEVIISAIRA
                     VIQLLAVGFLLDYIFGYQNPIFTALLMLFMIINASYNAAKRGKGINKGFVISFIAIGS
                     GTIITLSVLIFSGILKFVPNQMIPVGGMIISNSMVAIGLCYKQLLSEFRSKQEEVETK
                     LALGADILPASIDIIRDVIKTGMVPTIDSAKTLGIVSLPGMMTGLILAGTSPIQAVKY
                     QMMVTFMLLATTSIASFVATYLAYKIFFNNRKQLVVTKD"
     gene            complement(3840..4496)
                     /locus_tag="ACUBV3_001804"
     CDS             complement(3840..4496)
                     /locus_tag="ACUBV3_001804"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HHU3848282.1"
                     /translation="MSYLTFKHVDFKTDDKLVLNDINFAIDEGDFVSIVGPSGSGKST
                     VLKLASGLMSPTAGHIFFDGKDLNQLEPIESRKMISYCFQTPHLFGNTVEDNISFPYH
                     IRHEKVDYRRVDDLFQRFEMDQSYLKQDVKKLSGGEKQRIALIRQLLFEPKVLLLDEV
                     TSALDNHNKAIVEKVIKSLHDKGITILWITHDEEQSRRFANKVLKVVNGSIEAMEVIK
                     "
BASE COUNT         1567 a          851 c          670 g         1411 t
ORIGIN      
        1 ggtgcagcaa caaatattat aattatcaat aacatattca taactgacca tccacctttt
       61 gctaaattaa gagcattaat cggtccaatc aaaccactga taccaaaacc tgcactagct
      121 ggtgttcctt gaatattaaa gagagctccg agaattccta gtattgctgc tgaagatatc
      181 ataggtagca taatcttcgg ttttgctaat acattagcca tcgaaatctt aggagaccct
      241 aaaacatgcg ctaaggctgt ccccttagaa ttgactgacc acccagccat acaaagccca
      301 aaactagcag cacagatacc taaattagca gcaccagaac caattccgga caaactaatt
      361 gccaaagcaa tcccaactgt tgtgattgga gatacgatca agaaacagaa aatcatcgca
      421 attaaaattg acatcaaaac aggttgtaaa ccaagtaatg aagcaatccc ttgtccaatc
      481 atggtggtaa tcattttgac ataaggtaat aacacatgac ctactcctcc aactagtaag
      541 agtgttactg ttggaatgac aatcaaagtg aatgatttcg tcttgtcaga taaaaattgg
      601 atcaaaagga cacctaaggc agcagtaatt cccatgttaa tgatatcgcc tgttcctttt
      661 agcataatag cgcctttaag gaaggttgca gctccacctg caaacataac cgctaatcct
      721 agtgatgctg attgaatggg attaaattta aaattgagac caaccataat accaataaca
      781 agccccatca tacttgttgc aactgctgtt gcagcgatta aagttgcaaa gccgctccac
      841 attggcagta aagctttcat taattctcca aggattgctc ctggtatcaa gacaataaca
      901 gtccctaaag ctaaaccatt taatacgttc attataaacg atttagttgt ttgtttttcg
      961 acaacatccg acataagatg cctcctacta ataatgattg tttgtgattt cactaactat
     1021 attaactata tcatagctat ttgcaaaagg aaaattggaa aaaattatat aagtatagct
     1081 tttactaata ttattgtgga aataatcata aaggtcagat tgatagactt gtgctatact
     1141 attactaaga aaaaaggaca gatattatgg atattagaca acttacttac tttattgctg
     1201 ttgcagaagc aaaaaattat tcacgcgctg caaaaagtct ttttgtgacc caacctacac
     1261 tttcacaatc cattaaaaaa ttagaagcag aattaaatac tattttattc ttacaaaatg
     1321 gtcgtcaact cgctttaact gaagcaggag aaattctcta tgagaaagga caattgttga
     1381 tgactaatgt taatcaaatg gttacggaga tccagcaatt aaatcaagaa aaaaaggaag
     1441 gtatccgtgt agggcttact tctctttttg ctattcaatt tatgaaacag atttcaactt
     1501 ttatggctac acattcaaat gttgaagtat ctttgattca agatggttcc cgtaaattac
     1561 aagaattgtt agcaaaagga aagatcgata ttggcttatt gtcctttcca agtacccgca
     1621 atgatatcac tatcgaacca ctgcaaacct caactaaagg atataaagta agcattgtca
     1681 tgccaaaatc gcatcctctt gcaacacttc ctgaaattga acttaatgat ttaagagatt
     1741 ataaagttgc ctcactcaac gaacattata tgctaggaga gatgttgcct cgtaaatgtc
     1801 gtgctttagg atttgacccc catatcgtct ttaaacacaa tgactgggaa gttcttattc
     1861 atagtttaca agacctcaat gcagtcacta ttttaccaag tgaatttgaa agcattagcc
     1921 aagttcaaga tttatgctgg gtcccactta aggataaaaa taatttttat ccgattggta
     1981 ttgcttaccg aaatgatact tcttttagtc ctatgattga agaattttta agtcttttaa
     2041 agaccaatta atagcagtta aaccaaaaag ttttaaactt tttggtttta tttattttcc
     2101 aaatgacaaa gaaaatcata aactttttga ttccactcat aaagtgtttc gttacagtaa
     2161 tctaaataat aaaccctgtg tttcaagtca ttgggcacaa ggctttgata atcttcttga
     2221 taatgttttg ggataacaca tgcaactaaa tgttctgcta aagaaagctg atttttaatt
     2281 ttagcaggat gataaccatc aaaaatagaa tcaggatgat tgatgatatc ttgataagta
     2341 aaatgaaaac tgacccctgg cttaaaacct actgttaact cctgttcaga tggtgctttg
     2401 cctaacaatc tttccattac taaacgataa ccagaattgg tatttgacca gttcaacatc
     2461 acatagtcaa agtaatcttt agggtctcca gcagcattcc tcttatcttt taccagtact
     2521 tcagcaggct tattaaaggc tttaactgct gataagattt taccttcttt aaaaattagt
     2581 tttgcagttt caatcgtaca tgtatggctt aaaaaatcag aagttaaact aataccatcc
     2641 tcattaaaag tagctgccgc ttctctatat ttgggaatat agggaaaagt aaaactattg
     2701 tattttaaaa aatgaacaat ttttgcgagg agatgtttat cgtcacattg tatctcagtt
     2761 tttcttttat attgtttctc gtaagctatg aaagctccta tcctttccaa ttcaaacgcc
     2821 ttaattcgag gataatccag caaagctaat tggtaaacac ctccccattg taaactatat
     2881 gctgcacgac aaatttttaa aatcatttct tcctcctttt gataatccct acaacaattt
     2941 ctattaaata gtagcattta aacgtaatcg actcaataac ttggtcaatt ttcgatcaaa
     3001 aataagatta agataccaaa aaagcctgga ctatacttac cagactaaca tactgtttat
     3061 taaaatgaat caatcttttg ttactactaa ttgcttacga ttattaaaga aaattttata
     3121 agccaaataa gtagctacaa aagaagctat cgaagtcgtt gctaatagca taaacgtcac
     3181 catcatttgg tatttaaccg cttggatagg tgaagttcca gcaagaatta accctgtcat
     3241 catccctggc aaagatacaa tcccaagtgt tttagctgaa tcaattgtag gtaccatacc
     3301 agttttgata acatcacgaa taatatcaat cgaagctggc aaaatatctg ctcctaaagc
     3361 taattttgtc tccacttcct cttgtttact tctaaattcg gacaataatt gtttatagca
     3421 caaaccgatt gctaccatag aattactaat aatcatacca cctacaggaa tcatttgatt
     3481 cggaacaaat tttaaaattc ctgaaaaaat caagacagat aaagtgatta tagtccctga
     3541 cccaatagct ataaatgaaa taacaaaccc tttattaatc cctttaccac gcttagctgc
     3601 gttataagaa gcattaataa tcatgaatag cattaaaaga gctgtgaaaa taggattttg
     3661 ataaccaaat atatagtcta gcaaaaaacc cactgctaat aattgaatca ctgctcggat
     3721 agcgctaata atcacttctt tttctaggtt taacttttgc cagtaagaaa aaaataaagt
     3781 aatcaaaact aatgacgatg ccattagtaa tgacattaaa gaaatagaat ttgcaccgct
     3841 cattttataa cctccattgc ttcaatacta ccattgacaa cttttaagac tttattggca
     3901 aatcgacgac tctgttcctc atcatgcgta atccacaaaa tcgttattcc tttgtcatgt
     3961 aatgatttaa taaccttttc aacaatagct ttattatgat tgtctaaagc cgaagtaact
     4021 tcatctaaga gtaaaacttt aggttcaaac aataattgcc taatcagagc aatacgttgc
     4081 ttttctcctc ctgatagttt tttgacatcc tgtttcaggt aagactggtc catttcaaaa
     4141 cgttgaaata aatcatcaac gcgtctataa tctacttttt cgtgtcgaat gtggtaagga
     4201 aaactaatat tgtcttccac tgtattacca aataaatgtg gcgtttgaaa acagtaagaa
     4261 atcatttttc gactttcaat cggttctaat tggttcaaat cttttccatc aaagaaaata
     4321 tgacctgcag taggactcat taaaccactg gctaatttta atacagtact cttaccactt
     4381 ccagatggcc ctacaataga aacaaaatca ccttcatcaa ttgcaaagtt gatatcattt
     4441 aaaaccaact tatcatccgt tttaaaatca acgtgtttaa aggttaaata ggacattgt
//
LOCUS       DBJYSH010000126         4486 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.126, whole genome shotgun sequence.
ACCESSION   DBJYSH010000126 DBJYSH010000000
VERSION     DBJYSH010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 4486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4486
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.126"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            198..965
                     /gene="cfb"
                     /locus_tag="ACUBV3_001805"
     CDS             198..965
                     /gene="cfb"
                     /locus_tag="ACUBV3_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAMP factor pore-forming toxin Cfb-I"
                     /protein_id="HHU3848283.1"
                     /translation="MNVKHMMYLSGTLVAGALLFSPAVLEVHADQVTTPQVVNHVNSN
                     NQAQQMAQKLDQDSIQLRNIKDNVQGTDYEKTVNEAITSVEKLKTSLRANPETVYDLN
                     SIGSRVEALTDVIEAITFSTQHLANKVSQANIDMGFGITKLVIRILDPFASVDSIKAQ
                     VNDVKALEQKVLTYPDLKPTDRATIYTKSKLDKEIWNTRFTRDKKVLNVKEFKVYNTL
                     NKAITHAVGVQLNPNVTVQQVDQEIVTLQAALQTALK"
     gene            1138..1440
                     /locus_tag="ACUBV3_001806"
     CDS             1138..1440
                     /locus_tag="ACUBV3_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HHU3848284.1"
                     /translation="MTKKITVVALETLIAQHNNIHLIDVREEHEYRGGHISGAINLPL
                     SQLSHKFEQLDKNKEYYLVCQRGGRSIRACQFLELKGYKVINVDGGTEAWRGNLEV"
     gene            complement(1597..2460)
                     /locus_tag="ACUBV3_001807"
     CDS             complement(1597..2460)
                     /locus_tag="ACUBV3_001807"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HHU3848285.1"
                     /translation="MNQEKKRLNLIGLILLAQAIVLSLATTLFAEILQNDVWIGIAST
                     LIALLIPCFFMGFKNLKKHLTATHHTITFTTILFLLSIIYTTNNIVSTIWDVVLNHLN
                     IMESESQNYDATFNILMISYSVVVGPFFEEVLYRGIVLNLLSKYGKWFAIITSGILFG
                     LMHQDISQLLTTSIAGIIMGFIAYHYSFKVALLLHICNNFIVEIFTQLSTVNELYGTY
                     FENILLILAILFILYYLFTHRNTARRRISLHSNVREADSINSKQHTKLLLTSWPFILL
                     VIYDIVLTTIN"
     gene            complement(2765..3325)
                     /locus_tag="ACUBV3_001808"
     CDS             complement(2765..3325)
                     /locus_tag="ACUBV3_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HHU3848286.1"
                     /translation="MRITIFCGASTGENPVYSEKTVALAQWMAQNKHSLVYGGGKVGL
                     MGVMADTVIANGGYTTGVIPTFLRDREIAHENLSELIIVNNMPERKAKMMLLGDAFIA
                     LPGGPGTLEEISEVISWSRIGQNDNPCILYNVNGYFNDLKNMFDHMVGEGFLSLEDRE
                     NVLFSDDITEIEDFITNYKVPSTRQY"
     gene            complement(3342..3638)
                     /locus_tag="ACUBV3_001809"
     CDS             complement(3342..3638)
                     /locus_tag="ACUBV3_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HHU3848287.1"
                     /translation="MDITDYQKWVSEFYKKRNWYQYNSFIRSNFLSEEVGELAQAIRK
                     YEIGRDRPDETEQTDLENLNDIKEELGDVLDNIFILADQYNISLEEIISAHRTN"
     gene            3852..4466
                     /locus_tag="ACUBV3_001810"
     CDS             3852..4466
                     /locus_tag="ACUBV3_001810"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HHU3848288.1"
                     /translation="MNKKSFFQCVILLVSFLVLTLAVKSQSDMISYLDNITSAFFQSI
                     RNPDLTNLMTIISTVVSPLTTSLIALVILGYQYFLNQRIAVWLFMLFFGTNALALLLK
                     DIIARHRPMNQLVFDSGYSFPSGHTISTFLLMILVLVVARQRLRRVLSQVVFVIFALV
                     ILASVIFSRLYLENHFLTDILGSLLLGASSYYGLSAIVSLKELQ"
BASE COUNT         1535 a          676 c          788 g         1487 t
ORIGIN      
        1 ataggcgcaa gtatattata gcaaaatgat aatattagtt ttatgaaaaa aatgcagttt
       61 atttgtaata atgtaaaata taggggaaaa gaaagcgctt gacgaccttt tggacaagta
      121 gtaagataca aacatgggcc ctgtaaatta aaaatactgc agtaaaagtg attttagttt
      181 aaaggaggaa attcattatg aacgttaaac atatgatgta tctatctgga actctagtgg
      241 ctggtgcatt gttattttca ccagctgtat tagaagtaca tgctgatcaa gtgacaactc
      301 cacaagtggt aaatcatgta aacagtaata atcaagccca gcaaatggct caaaagcttg
      361 atcaagatag cattcagttg agaaatatca aagataatgt tcagggaaca gattatgaaa
      421 aaacggttaa tgaggctatt actagtgttg aaaaattaaa gacttcattg cgtgccaacc
      481 ctgagacagt ttatgatttg aattctattg gtagtcgtgt agaagcctta acagatgtga
      541 ttgaagcaat cactttttca actcaacatt tagcaaataa ggttagtcaa gcaaatattg
      601 atatgggatt tgggataact aagctggtta ttcgcatttt agatccattt gcttcagttg
      661 attcaattaa agctcaagtt aacgatgtaa aggcattaga acaaaaggtt ttaacttatc
      721 ctgatttaaa accaactgat agagctacca tctatacaaa atcaaaactt gataaggaaa
      781 tctggaatac acgctttact agagataaaa aagtacttaa cgtcaaagaa tttaaagttt
      841 acaatacttt aaataaagca atcacacatg ctgttggagt tcagttgaat ccaaatgtta
      901 cggtacaaca agttgatcaa gagattgtaa cattacaagc agcacttcaa acagcattaa
      961 aataatattt gtatttttcg tgtgatgctg tcgacttcgt gattttgtac taccatggtt
     1021 tttatgatta aaaggtttac gataatagtc ataatagtag aacgatgtca ccattttaaa
     1081 taataaagtg attagtcatt tgactaaatt tgccaagtat caaaggaaat aaagattatg
     1141 actaaaaaga taactgttgt agcattagaa acattgattg cccagcataa taatatccat
     1201 ttgatagacg ttcgtgaaga gcatgagtat cgtggggggc atatttcagg tgcgataaat
     1261 cttcctttga gtcaactcag tcataagttt gaacagttag ataaaaataa ggaatattat
     1321 cttgtttgtc aacgaggggg aagatctatt agagcatgcc aatttcttga gttgaaaggc
     1381 tacaaagtaa tcaatgtaga tggcggcaca gaagcgtgga gaggaaatct agaagtgtaa
     1441 atatgtcctg tgtagtctac catatttgat aataaaaaaa ttgacattat ctcactatta
     1501 atgacctttt agtaaacata aagaaaagta tgtgttttga aaaggaggat gattatcctt
     1561 ttcaaaacca tactttttgt gtcttgtggc ttggtcttaa ttaatagttg ttaggacaat
     1621 gtcgtaaatg acaaggagaa taaaaggcca actagtaagt aataattttg tatgttgttt
     1681 agagtttatt gagtcagcct ctcgaacgtt agagtgcaga cttatacgac ggcgagcagt
     1741 attcctatga gtaaaaaggt aatataatat aaataagatt gctaaaatta gcaatatatt
     1801 ttcaaaataa gtcccgtata actcgtttac tgttgatagc tgggtgaaaa tttctacgat
     1861 aaagttattg cagatatgaa ggagtagggc taccttaaag gaataatggt aagcgataaa
     1921 acccattatg atacccgcaa tagatgtagt caatagctga gaaatgtctt ggtgcattaa
     1981 accaaatagg atgccagaag tgataatggc aaaccattta ccatacttgg ataataaatt
     2041 caatactatt cctcggtata aaacttcttc aaaaaaagga cctacaacaa cagaataact
     2101 aatcataagt atattgaatg tggcatcata attttgactt tccgattcca taatattgag
     2161 atgatttagc acaacatccc atattgtgga gactatatta ttagtagtat atataatgct
     2221 taacaagaat aaaatagtgg tgaaagtgat tgtatgatgg gttgccgtta gatgtttttt
     2281 taaattttta aaacccataa aaaaacaagg aatgagtaag gcgattaaag tacttgctat
     2341 acctatccag acatcatttt gtaagatttc agcaaatagt gtagtcgcca aacttaaaac
     2401 aatagcctga gctaaaagta tcagcccgat taggttaagt ctcttttttt cttgattcaa
     2461 ttgattacct ccttattaat aacggtaacg acaaagaaat attataatgt ataatgtata
     2521 ggtttctaag tcattagtca atagggacga gcacttggat acttctaata agataaaata
     2581 catagacaag actatagttt tggcttctct aaagattacc taaaaattta taagttctcg
     2641 agcagaaagg atgagtttgg atattgagag gatgggcttg accagctgaa agaaaaatga
     2701 accaagggta atcgcttcga aaaaagaagg aaatttcaat tagaaacttc ctttttttat
     2761 caatctagta ttggcgcgtg ctagggactt tataattcgt tataaaatct tcgatttcag
     2821 taatatcatc tgagaatagg acattttctc tatcttccag actaagaaaa ccctcaccaa
     2881 ccatatgatc aaacatattt tttaaatcat taaagtagcc attaacatta tataaaatgc
     2941 aaggattatc attttgaccg atgcgagacc aagagataac ttcggaaatt tcttcaaggg
     3001 taccaggtcc accaggtaga gcgataaagg catctccaag tagcatcatt tttgccttac
     3061 gttcaggcat attatttact atgataagct ctgataaatt ttcatgtgca atttcacgat
     3121 ctcttaaaaa tgtcggaata acaccagtag tgtagccacc atttgcaatc acagtatcag
     3181 ccatgactcc cattaagcca acttttcctc caccatacac cagagaatgt ttgttttgtg
     3241 ccatccattg tgctaaggca actgtttttt cactataaac aggattttcc ccagtacttg
     3301 ctccacaaaa aatagttatt ctcatgtttc aaacctcttt tctaattagt gcgatgtgcg
     3361 ctgataatct cttctagtga gatattatac tgatcagcaa gtatgaagat attatctaag
     3421 acatctccta gttcttcttt tatatcattt aagttttcca aatcagtttg ttcggtttcg
     3481 tcaggtctgt cacgtccaat ttcatatttg cgaatggctt gagccaattc gccgacttct
     3541 tctgaaagaa agttacttct aataaaagaa ttgtattgat accagtttct ttttttatag
     3601 aattcactca cccatttttg ataatctgta atatccatta ttccctccgt ttcaagtata
     3661 ttgtagcaat attgctttat taatgaaaat ttgaacgcta attaatagga aaagttcgta
     3721 tttggtattt cttctagttt gtgaatagtc atatttaaac ctaaaaggtt agcgcgtact
     3781 ttaagcattt tttaaatatt ttaaaccata atgtatgaag atttatctct ttattaaagt
     3841 gaggttatgt agtgaacaag aaatcctttt tccagtgtgt tatactactt gtttcttttt
     3901 tagtgttaac attagcagtt aaatctcaaa gtgatatgat aagttatttg gataatatca
     3961 cttcagcttt ttttcaaagc atacgaaatc ccgatttaac taacttaatg acgataatat
     4021 caacagttgt cagccctttg acaacgtcat taatagcact tgttatcctt gggtatcaat
     4081 attttttaaa ccaaagaata gctgtgtggc tatttatgtt attttttggt accaatgctt
     4141 tggcattgct tttaaaagat atcatcgcac gtcatagacc tatgaatcaa cttgtttttg
     4201 attcgggtta tagttttccc agtggacata cgatatcaac gtttttatta atgattcttg
     4261 ttttagtggt tgcgagacag agattgagaa gagtattgtc acaagtagtt ttcgtaatat
     4321 ttgcattagt gatccttgca tcagttattt tttcaagact ctatttagaa aatcattttt
     4381 taacggatat tttagggtcg ttgttactag gagcatcgtc atattatggt ttgtctgcta
     4441 ttgtatcttt gaaagagtta caataaaaat agtagttacc aatgaa
//
LOCUS       DBJYSH010000127         4373 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.127, whole genome shotgun sequence.
ACCESSION   DBJYSH010000127 DBJYSH010000000
VERSION     DBJYSH010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 4373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4373
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.127"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            complement(112..645)
                     /gene="ntdP"
                     /locus_tag="ACUBV3_001811"
     CDS             complement(112..645)
                     /gene="ntdP"
                     /locus_tag="ACUBV3_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002949762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017110 - nucleoside diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0017111 - ribonucleoside triphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside tri-diphosphate phosphatase"
                     /protein_id="HHU3848289.1"
                     /translation="MRLPKEGDFITIQSYKHDGSLHRTWRDTMVLKTTENALIGVNDH
                     TLVTENDGRRWVTREPAIVYFHKKYWFNIIAMIRETGVSYYCNLASPYILDPEALKYI
                     DYDLDVKVFADGEKRLLDVDEYEQHKAQMNYPTDIDYILKENVKILVEWINENKGPFS
                     SSYINIWYKRYLELKKR"
     gene            complement(729..1505)
                     /gene="recX"
                     /locus_tag="ACUBV3_001812"
     CDS             complement(729..1505)
                     /gene="recX"
                     /locus_tag="ACUBV3_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HHU3848290.1"
                     /translation="MKITKIEKKKRLYTLELDNTENLYITEDTIVHFMLSKGMVINAE
                     KLENIKKFAQLSYGKNLGLYYISFKQRTEKEVIKYLQQHDIDSKIIPQIIDNLKSENW
                     INDKNYVQSFIQQNLNTGDKGPYVIKQKLLQKGIKSKIIESELQAINFQDLASKISQK
                     LYKKYQSKLPLKALKDKLMQSLTTKGFDYQIAHTVIQNLEIEKDQELEEDLIYKELDK
                     QYQKLSKKYDQYELKQRIINALMRKGYQYEDIKSALREYL"
     gene            1603..2958
                     /gene="rlmD"
                     /locus_tag="ACUBV3_001813"
     CDS             1603..2958
                     /gene="rlmD"
                     /locus_tag="ACUBV3_001813"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HHU3848291.1"
                     /translation="MNVVLKQRIPLKIKRMGINGEGIGFYKKTLIFVPGALKGEEVFC
                     QISSVRRNFAEAKLLKINKKSKNRVDPACSIYKECGGCQIMHLQYDKQLEFKTDVIRQ
                     ALMKFKPEGYENYEIRKTIGMPEPEHYRAKLQFQVRSFGGNVRAGLYAQGTHRLIDIK
                     DCLVQDRLTQEMINRVAELLGKYKLPIYNERKIAGVRTVMIRRAQASGEIQLIFITSK
                     RLDFDDVVIELVREFPELKTVAVNINASKTSDIYGQITEVIWGQESINEEVLDYGFSL
                     SPRAFYQLNPKQTQILYSEAVNALDVKEDDDLIDAYCGVGTIGLAFAGKVKSVRGMDI
                     IPEAIQDAKENALHMGFTNTHYEAGKAEDIIPRWYSEGFRANALIVDPPRTGLDDKLL
                     NTILKMPPEKMVYVSCNTSTLARDLVTLTKVYHVHYIQSVDMFPHTARTEAVVKLQRK
                     E"
     gene            3226..3990
                     /locus_tag="ACUBV3_001814"
     CDS             3226..3990
                     /locus_tag="ACUBV3_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HHU3848292.1"
                     /translation="MIINSFDNSRKAIINPEDINSPIKGFPKTVITCFARETFNRILE
                     ELPHREIARTSVANLEIPIYELEFKGQKIGFFNAYVGASACVAILEDIIVFGMENLIV
                     FGTCGVLDSSIEETSIIIPRSAIRDEGTSFHYSEASSEIAVNTKFHEEFKSFLDRQAV
                     SYTEGKVWTTDGIYRETVDKMLEHKRNGAICVDMECSAIAALSKFRNINHFQFFYSAD
                     NLDAEVWEARSLGNSEDLEKKDKIAYIAMEFAIETF"
     gene            4024..4335
                     /locus_tag="ACUBV3_001815"
                     /pseudo
     CDS             4024..4335
                     /locus_tag="ACUBV3_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768361.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT         1410 a          587 c          815 g         1561 t
ORIGIN      
        1 ttaaaaaagt agaaagatca ctttctactt tttttatttt aagtatatat ttttgcaata
       61 gtattgtaat taaatttgat attaataaaa acacactatt tagtgtgtta gttagcgctt
      121 tttcaattca aggtaccgtt tataccagat attgatatat gatgatgaaa aggggccttt
      181 attctcattt atccattcta ccaatatttt tacattttcc tttaatatat aatcaatatc
      241 ggtaggatag ttcatctgag ctttatgctg ttcatattcg tccacatcta gtagtctttt
      301 ttcaccatca gcaaatactt tgacatcaag gtcatagtca atatacttga gtgcttcagg
      361 gtctaagata tacggacttg ctagattaca atagtaggag acaccagttt cacgtatcat
      421 agcgataatg ttaaaccagt attttttatg aaagtatact attgcaggct ctcgtgtcac
      481 ccagcgtcga ccatcatttt ctgttactaa agtatgatca ttaacaccaa tgagggcatt
      541 ttcggttgtt tttaatacca tggtgtcacg ccaagtccgg tgtaaactac catcatgttt
      601 gtaactttga attgtaataa agtcgccttc tttaggtaat ctcatagcct accaactttc
      661 tacaatttat acgtctgtta tagtttatca taattttctg taaaatacat cgtctgagag
      721 ttattatctt ataaatattc tcttaaagca cttttgatat cttcgtactg gtatccttta
      781 cgcattaagg cattgattat acgttgcttt aattcatact ggtcatattt ttttgataat
      841 ttttggtatt gtttatctaa ttctttataa atgaggtctt cttcaagttc ttgatctttt
      901 tcaatttcaa ggttttgaat tacggtgtgc gctatctgat aatcaaatcc ttttgtagtt
      961 aagctctgca tcaatttatc ttttaatgct tttagtggaa gtttgctttg atatttttta
     1021 tagagttttt ggcttatttt ggaggctaag tcttgaaagt ttatagcttg aagttcagat
     1081 tcaattattt tacttttgat acctttttgc aataattttt gttttatcac gtatggcccc
     1141 ttatcgccag tatttagatt ttgctgtatg aaagattgca cgtagttttt atcgtttatc
     1201 caattctcac tttttagatt atcaataatt tgtggaataa ttttagaatc aatatcatgt
     1261 tgttgaagat atttaataac ttctttctcg gttctctgct tgaaagaaat ataataaaga
     1321 cctagatttt taccgtatga tagttgggca aattttttaa tattttcaag tttctcggca
     1381 ttaattacca tccctttact taacataaaa tgaacgatag tatcttctgt tatatataaa
     1441 ttttcggtat tgtctagctc tagagtgtaa agtctttttt tcttttcgat tttggttatt
     1501 ttcatagttt ttattatagc aaaaatgata aaatagtaaa aatgagtctt ttttaaatgt
     1561 tatggattta tagtattttc ttagttaggg agtaatgaac tgatgaatgt ggttttaaaa
     1621 cagcgaattc ctttaaaaat taaacgaatg ggaattaatg gtgaaggaat aggtttttat
     1681 aaaaaaacac taatctttgt tccaggggct cttaagggag aagaggtgtt ttgtcagatt
     1741 tcttctgtta gacgtaattt tgcagaagct aaactgttaa agataaataa aaagtctaaa
     1801 aatagggtag atcccgcttg ttctatttat aaagaatgtg ggggatgcca aataatgcac
     1861 cttcaatatg ataagcagct agagtttaaa acggatgtta ttcgacaggc tctaatgaaa
     1921 tttaaaccag agggatatga gaattatgaa atccgtaaaa cgataggtat gccggaacct
     1981 gaacactatc gtgctaaatt acaatttcaa gtgcgttcgt ttggagggaa tgtgagggca
     2041 ggtttatatg cacaagggac acatcgattg attgatatca aagattgttt agtgcaagat
     2101 aggcttactc aggaaatgat aaatcgagtt gcagaacttt taggaaaata taaattgcct
     2161 atttataatg aacgtaaaat agctggtgtc cgtactgtta tgattcgacg agcacaagcg
     2221 agtggagaga ttcaactgat ctttatcaca agtaaaagac ttgattttga tgatgttgtt
     2281 attgaattag ttcgagagtt tcctgagtta aagacagttg ctgtaaatat caacgctagt
     2341 aaaacaagtg atatctatgg tcaaataaca gaagttattt ggggtcaaga atctatcaat
     2401 gaagaagtat tagattatgg tttttcgtta tcaccacgtg ctttttatca acttaatcct
     2461 aaacaaacac aaattttata tagtgaggca gttaatgcct tagatgtgaa ggaagatgat
     2521 gatttaattg acgcttattg tggagtagga acgattggac ttgcttttgc tggaaaagtt
     2581 aaaagtgttc gtggaatgga tattatccct gaggctattc aggatgctaa agagaacgca
     2641 cttcatatgg gttttactaa tacacattac gaagctggaa aggcagagga tattatccct
     2701 aggtggtata gtgagggatt tcgcgctaat gcgttaatcg tggatcctcc tcgcacaggt
     2761 ttagatgata aattgctaaa tactatacta aaaatgcctc cagaaaaaat ggtttacgtt
     2821 tcttgtaata catccacgct tgctagagac ttagtaacat taacaaaagt ataccatgtc
     2881 cactacatcc aatccgttga catgtttcca catacagcaa ggaccgaggc tgtagtgaaa
     2941 ctacaacgta aggaataata aagttttcta atcaaaaaat aagagtattt ttgggtattc
     3001 ttgtttttag atagtaatgg gagatattaa tataacatag tataaaaaca catggtttaa
     3061 gctatttgat acggaaattt accttagaaa tggtaaatag ttccattaag tatattcatc
     3121 tttgcaaatt tgaccttgcc ttttaagttt cattgagtta aaatagaaaa aatattttta
     3181 acaagatgat agaacatcgt aaattaaggt ttaaaggaaa tgcttatgat aataaattca
     3241 ttcgataata gtagaaaagc gattattaat cctgaagata ttaattcacc aataaaaggt
     3301 tttccaaaga ctgttattac ctgttttgcg cgggagactt tcaatcgtat tttagaagaa
     3361 ttgcctcatc gtgaaattgc tagaactagt gtagcaaact tagaaattcc catttatgaa
     3421 ttagaattta aaggacaaaa gattggcttt ttcaatgctt atgttggtgc ttcagcttgc
     3481 gtagcaatct tggaagatat tatagtgttt ggaatggaaa atttaattgt ttttggaaca
     3541 tgtggcgtcc ttgattcgtc aattgaggag acatccatta ttattccaag aagtgctatt
     3601 agggatgaag ggacatcatt tcattattcg gaagcaagtt ctgaaattgc agtaaacaca
     3661 aaatttcatg aagagtttaa atctttttta gatcgtcaag ctgtttctta cactgaagga
     3721 aaagtttgga caacggatgg tatttaccgt gagacagttg ataaaatgtt ggaacataaa
     3781 cggaatggag caatctgtgt ggatatggaa tgttcagcaa ttgcagcttt atctaagttt
     3841 cgaaatatta atcattttca atttttttac tctgcggata atttagatgc agaagtatgg
     3901 gaagcaagat ctttaggaaa tagcgaggat ttagaaaaga aagataaaat tgcttatatt
     3961 gccatggagt ttgctataga gactttttag ggtctactgt atgatgttga aaggaagata
     4021 gcaatgtgga atgtaaaaac ttttaataat ttaactacac atgagttgtt tcaaatatat
     4081 aaattaagag tatctgtatt tgtggttgaa caagattgtc cttatcaaga ggttgatgat
     4141 gaagatttga tttgtctgca tgggatgaat tgggtagatg gtcaattggc ggcttattat
     4201 agattgatac cagaagatga taaggtgcat ttgggacgtg tgattgttaa tccagacttt
     4261 agaaaaaagg gattaggaaa tcaattagtg gaatatgcga ttaaatttag tgaggcaaat
     4321 taccctaata aacctaatat ggttttatca taaatacata ctatttaaac tat
//
LOCUS       DBJYSH010000128         4339 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.128, whole genome shotgun sequence.
ACCESSION   DBJYSH010000128 DBJYSH010000000
VERSION     DBJYSH010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 4339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4339
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.128"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            73..1104
                     /locus_tag="ACUBV3_001816"
     CDS             73..1104
                     /locus_tag="ACUBV3_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HHU3848293.1"
                     /translation="MKLSKQLYKSLPLLTVVLCVGALQQNVEAKAKHYKTTSHVETQY
                     VSTSSKKILPFTHNKQIKVGPLDNLGRATYSHIQLRDADEPKIKRERLTYNPTGWHNY
                     KFTTEKGKTTWLMDRGHLVGYQFSGMNNVPENLVTMTKYLNTGFSENNPDGMLYYENR
                     LDSWLANHKNFWLDYKVTPIYEGNNLVPSRVELQYVGIDKQGKLLEIKLGGGKEQTDE
                     YGVTTVTLENTSPLAKIDYKTGMLIKEDGKQAEEGEDPNSDADENEAAIESASDIEEN
                     TNTNTSESDTNNVAPKNRIVYVANKGRSNTYWYSLENIKNANTANIVQMTEQEALNQH
                     KHHSTTEAQ"
     gene            complement(1316..2128)
                     /locus_tag="ACUBV3_001817"
     CDS             complement(1316..2128)
                     /locus_tag="ACUBV3_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000923271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HHU3848294.1"
                     /translation="MLLDMLSYDFMQRALLAVVAISIFAPILGIFLILRRQSLMSDTL
                     SHVSLAGVALGVVLGISPTWSTIFVVTLAAVVLEYLRTVYKHYMEISTAILMSMGLAI
                     SLIVMSKAHNVGNVSLEQYLFGSIITIGKEQVIALFVIALITFILTILFIRPMYILTF
                     DEDTAFVDGLPVRTMSILFNVVTGIAIALTIPAAGALLVSTIMVLPASIAMRLGRNFK
                     TVIFLGMLIGFVGMVAGIFLSYYWETPASATITMIFIGIFLLVSLVGLLRKR"
     gene            complement(2118..2828)
                     /locus_tag="ACUBV3_001818"
     CDS             complement(2118..2828)
                     /locus_tag="ACUBV3_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HHU3848295.1"
                     /translation="MRYITVSGLTFQYDSDPVLEGVNYHLDSGEFVTLTGENGAAKST
                     LIKATLGILTPKVGTVNISKENKEGKKLRIAYLPQQIASFNAGFPSSVYEFVKSGRYP
                     RNGWFRRLTKHDEEHIRVSLEAVGMWDNRHKKIGSLSGGQKQRAVIARMFASDPDIFV
                     LDEPTTGMDAGTTEKFYELMHHNAHKHGKSVLMITHDPDEVKGYADRNIHLVRNQSLP
                     WRCFNVHTNEMEVESDAT"
     gene            complement(2831..3274)
                     /locus_tag="ACUBV3_001819"
     CDS             complement(2831..3274)
                     /locus_tag="ACUBV3_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent MarR family transcriptional
                     regulator"
                     /protein_id="HHU3848296.1"
                     /translation="MTVLEQKLDHLVSQILLKAENQHELLFGTCQSDVKLTNTQEHIL
                     MLLSQEQLTNSDLAKKLNISQAAVTKAVKSLISQDMLKANKDSKDARITYFELSELAK
                     PIADEHTHHHDNTLGVYGRLVNHFSKDEKVVLERFLDLFSRELEG"
     gene            complement(3360..4211)
                     /gene="ispE"
                     /locus_tag="ACUBV3_001820"
     CDS             complement(3360..4211)
                     /gene="ispE"
                     /locus_tag="ACUBV3_001820"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HHU3848297.1"
                     /translation="MRIFEKAPAKLNLGLDIKGRCDDGYHELAMIMVSIDLNDYVTIS
                     ELKEDCIVIDSDSSKMPLNNDNDVFKAADIIKNQYGINKGVHIRLEKSIPVCAGLGGG
                     STDAAATIRALNRLWNLQMDYDEMVAIGFKIGSDVPYCLGGGCSLVLGKGEIVKPLPT
                     LRPCWIVLVKPDFGISTKSIFRDIDCKSISRVDIDLLKSAILSSDYQLMVKSMGNSLE
                     DITITKNPVISTIKERMLNSGADVALMTGSGPTVFSMCSTEKKADRVFNSMKGFCKEV
                     YKVRLLR"
BASE COUNT         1598 a          854 c          638 g         1249 t
ORIGIN      
        1 ttttcccaat aaaatgataa aattatactt gtgaaaggcc ttcacaaata tcatttagaa
       61 gggtttaggt ttatgaaatt atctaaacaa ttatacaaat cactcccact attaactgtc
      121 gtgctatgcg ttggggcatt acaacagaat gttgaagcta aggctaaaca ttataagacg
      181 acatcccatg ttgagacaca gtatgtaagt acttcttcaa aaaaaatctt accatttact
      241 cataataaac aaatcaaggt aggtccatta gataacttag gcagagcaac atattctcac
      301 atacaattga gggatgcaga tgagcctaag ataaaaagag aaagactgac gtataatcca
      361 accggttggc acaactacaa atttactact gaaaagggta aaacaacttg gcttatggac
      421 cgaggtcacc tcgttggtta tcaattttca ggaatgaaca atgttcctga gaacctagtt
      481 actatgacca aatatctaaa tacaggattt agcgaaaata atcctgatgg tatgttatac
      541 tacgaaaata gattagatag ttggttggca aatcataaaa atttctggct tgattacaaa
      601 gtgactccaa tatacgaagg aaacaattta gtacctagcc gagttgaact tcagtatgtt
      661 ggaattgata aacaaggaaa acttctagaa atcaaattag gcggcggcaa agaacagact
      721 gatgaatacg gcgtcacaac tgttacatta gaaaatacgt ctccactcgc aaaaattgat
      781 tacaaaactg gtatgctaat caaagaagat gggaaacagg cagaagaagg tgaagatcca
      841 aatagcgatg ctgatgaaaa cgaagcagct attgaaagtg catctgatat agaagaaaat
      901 actaacacta atacttctga atcagataca aataatgtag ctcccaaaaa tagaatcgtc
      961 tacgttgcaa ataagggtcg ttcaaatact tattggtata gtttagaaaa cataaaaaat
     1021 gcaaataccg ctaatatcgt tcaaatgact gaacaagaag cgttaaatca acacaaacat
     1081 cacagcacta ctgaagcaca ataattacaa tttactgccg gcaaaattgc cggtagtatt
     1141 ttttatccta aaacgcaaaa atagattcac attgcccctt ctatcaactt aagccagaaa
     1201 acttagtaga tacctcatcg agataatttc tcaactgatc cctcataaaa tatattcgaa
     1261 aaaaaacagg gagataaata aaaaatctat cctagaataa gatagatttt tttatttaac
     1321 gtttgcgaag caagcctact aagctaacta ataggaaaat gccaataaaa atcatcgtta
     1381 ttgttgcact agcaggtgtc tcccaataat acgaaaggaa aataccagct accattccca
     1441 caaatccaat taacataccc agaaaaataa cagttttaaa atttcgacct aaccgcatag
     1501 caatactagc tggtaaaacc ataatagttg acaccaatag agcacctgcg gcaggaattg
     1561 ttaatgcaat agcaatacct gtaacaacat tgaacaaaat agacattgta cgcacaggca
     1621 atccatctac aaaagctgta tcctcatcaa acgttaatat atacattggt ctaataaaca
     1681 ggatggttaa aataaaagtg attaatgcaa taacaaacaa agcaatcact tgttccttcc
     1741 caatagtgat aatagaacca aataaatact gttcaagact gacattacca acattatgag
     1801 ctttactcat aacgattaaa gatatagcca accccatcga catcaggata gcagttgata
     1861 tttccatata atgtttgtat acagtacgca aatactctaa tacaactgcc gccaaagtaa
     1921 caacgaaaat agttgaccat gtaggcgaga tacctaaaac aacgcctaat gcaacaccgg
     1981 ctaaggaaac gtggctcaaa gtatcactca tcaaactctg acgacgtaaa attaggaaga
     2041 tgcctaaaat tggagcaaaa atactaatag ccacaactgc taagagcgct cgttgcataa
     2101 aatcatagga aagcatatca agtagcatca gattcaacct ccatttcgtt tgtatggaca
     2161 ttgaaacaac gccatggtaa agattggttc ctaacaagat gaatatttcg gtcagcatac
     2221 ccctttactt catcaggatc gtgagtaatc atcaaaacag acttaccatg cttatgagca
     2281 ttatgatgca ttaactcata aaatttctca gtagtccctg cgtccatacc agttgttggc
     2341 tcatccaata caaaaatatc tggatcagaa gcaaacatcc tagcaatgac agccctctgc
     2401 ttttgaccgc cagataaact tcctatcttt ttatggcgat tatcccacat accaacagct
     2461 tctaaactaa ccctaatatg ttcttcatca tgtttagtca gtcttctaaa ccaaccattt
     2521 ctagggtatc gtccagattt cacaaattca taaactgacg atggaaagcc agcgttaaaa
     2581 cttgcaatct gttgaggtaa ataggcaata cgtaattttt ttccttcttt gttttcttta
     2641 gagatattaa ctgtacccac tttgggcgtt aaaataccta aagtagcctt tattaaagtt
     2701 gatttagcag ccccattttc acctgttaag gtaacaaact ccccactgtc taaatggtaa
     2761 ttaacccctt ccagcacagg atcgctatca tactgaaaag taagaccact aactgtaata
     2821 tatcgcatga ttacccttct aattctctag agaaaagatc aagaaaacgt tctaaaacga
     2881 ccttttcgtc ctttgaaaaa tgatttacta atcttccgta aacccctaac gtattgtcat
     2941 gatgatgtgt gtgttcatct gcaatgggtt tagcaagttc agataactca aaataagtaa
     3001 tacgagcatc tttagaatct ttattcgctt tcaacatatc ctgagaaatt aaacttttta
     3061 ctgctttagt tacagctgcc tgactaatat ttaacttctt agctaaatca gaatttgtca
     3121 actgctcttg tgataaaagc atcagaatgt gttcttgcgt attagtcaat tttacatcac
     3181 tttgacaagt accaaacaat aattcatgtt gattttctgc tttaagcaag atttgactca
     3241 ctaaatggtc taatttttgt tctaaaactg tcatatatac ctcttttttg ttaaccagta
     3301 aattatatca cgaagatata gaagaatcaa tcatagatag gtgaagaagg taaaaccttt
     3361 tatctcaaca acctaacttt ataaacttct ttgcaaaaac ctttcatact attaaaaaca
     3421 cgatcagctt ttttctctgt agaacacatt gaaaaaacag ttggtccact tcctgtcatt
     3481 aatgcaacat cggctccaga atttaacata cgttctttta ttgtacttat aactggattt
     3541 ttagtaattg taatatcctc gagtgaattt cccatagatt tgaccattaa ctgataatct
     3601 gatgacaaaa tagcagactt taataaatca atatcaactc tgcttataga cttacaatca
     3661 atatctctaa aaatggattt agttgaaata ccaaaatccg gcttaaccag aactatccaa
     3721 catggtctca atgtcggtaa gggtttaaca atttcacctt tacctaatac taacgaacat
     3781 cccccaccaa gacaataagg aacatcacta ccaattttaa aaccaatagc aaccatttcg
     3841 tcatagtcca tttgaagatt ccataatcga ttaagagctc ttattgtagc agcagcatca
     3901 gtagaaccac cccccagtcc tgcacagaca ggaatggatt tttctaatct aatatgaaca
     3961 cctttattaa taccatattg atttttgatt atatctgcag ctttaaacac atcattatca
     4021 ttatttaaag gcattttgct actatcagaa tcgataacaa tacaatcttc ctttagctca
     4081 gaaatggtaa cgtagtcatt aagatcaata ctaaccataa tcatagctaa ttcatgataa
     4141 ccatcgtcac atcgtccttt aatatctaat cctaaattaa gtttggcagg agctttctca
     4201 aaaattctca taaaacctcc ctaataaaat atagaatatc catattataa cataacaaat
     4261 gacaaattcg gacaattaag atgttagcca atcttaagga taataattcc aataaaaaaa
     4321 ggctaaccaa agttagtct
//
LOCUS       DBJYSH010000129         4219 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.129, whole genome shotgun sequence.
ACCESSION   DBJYSH010000129 DBJYSH010000000
VERSION     DBJYSH010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 4219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4219
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.129"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            complement(<1..1816)
                     /locus_tag="ACUBV3_001821"
     CDS             complement(<1..1816)
                     /locus_tag="ACUBV3_001821"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HHU3848298.1"
                     /translation="MSKFLEKLKTDILVADGAMGTLLYTYGLDTCHESYNVTHPEKVL
                     AIHQAYIEAGADVIQTNTYGAQRHRLKNYGLEDQVVSINQAAVNIAHQATLGKETFIL
                     GTVGGFRSQRQCDLTLDNIVEETLEQVEALLATGQLDGLLFETYYDIEEITTVLKIVR
                     EMTDLPIITNISLHEAGVTSNGKPIVEALSQLVMLGADVIGLNCHLGPYHMIQSLKQV
                     PLFAQSYLSVYPNASQLSLDGENSQYQFSQNSEYFGKSAELLVAEGVRLIGGCCGTTP
                     DHIRAVKRSIRGLKPIERKVVTPIIPVKDFVRRIRRTDTLVDKVKKEVTIIAELDPPK
                     HLDIVQFQKAIRAIDQKGIAAITLADNSLSNTRICNLSIASLLKDEISTPFLLHIACR
                     DHNLIGLQSRLLGMELLGFNHILAITGDPTKLGDFPGATSVYDVTSFKLLSLIKQLNQ
                     GLSYSGASLRRPTDFTVAAAFNPNVKNLTRTVKLIEKKVASGADYFMTQPIFDHSVLK
                     ELADLTKTVEQPFFIGIMPITSYNNAVFLHNEVPGIKLSESFLSALEKVKDDKEACLT
                     LALNESKSLIDEALNYFNGIYLITPFLRYDLTLELIDYI"
     gene            complement(1861..4098)
                     /gene="metE"
                     /locus_tag="ACUBV3_001822"
     CDS             complement(1861..4098)
                     /gene="metE"
                     /locus_tag="ACUBV3_001822"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019806054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HHU3848299.1"
                     /translation="MVKVSNLGYPRLGEQREWKQAIEAFWAGNLEQKDLEKQLKQLRI
                     NHLKKQKEAGIDLIPVGDFSCYDHVLDLSFQFNVIPKRFDEYERNLDLYFAIARGDKD
                     NVASSMKKWFNTNYHYIVPEWEVETKPHLQNNYLLDLYLEAREVVGDKAKPVITGPIT
                     YVSLSSGIVDFEATVQRLLPLYKQVFQDLIDAGATYIQIDEPIFVTDEGELLVDIAKS
                     VYDFFAREVPQAHFIFQTYFESAVCLDKLSKLPVTGFGLDFIHGRAENLAAVKQGLFR
                     EKELFAGIVNGRNIWAVNLEETLALLEEIGPFVKRLTLQPSSSLLHVPVTTKYETHLD
                     PVLKNGLSFADEKLKELELLASAFDGNKTKGYHEALSRFSALQAADFRHVALESLAEV
                     KLERSPYKLRQALQAEKLQLPILPTTTIGSFPQSPEIRKKRLAWKRGNLSDSDYKDFI
                     KTEIRRWIAIQEDLDLDVLVHGEFERVDMVEFFGQKLAGFTTTKLGWVQSYGSRAVKP
                     PIIYGDVKHIQPLSLEETVYAQSLTKKPVKGMLTGPITITNWSFERDDISRSDLFNQI
                     ALAIKDEIQLLEQSGIAIIQVDEAALREGLPLRQQKQQAYLDDAVAAFKIATSSVKDE
                     TQIHTHMCYSKFDEIIDSIRALDADVISIETSRSHGDIIESFETAVYPLGIGLGVYDI
                     HSPRIPTKEEIIVNIQRSLKCLSKEQFWVNPDCGLKTRREAETIAALEVLVSATKEVR
                     QQLDN"
BASE COUNT         1333 a          838 c          689 g         1359 t
ORIGIN      
        1 gtatatagtc aattaactca agagtcagat catagcgtaa aaagggagta atcaaataga
       61 taccattaaa atagttcaag gcttcatcaa tcaaggactt actctcgttt aaggccaaag
      121 ttaaacaagc ttctttgtcg tcctttacct tttcaagagc agataaaaat gactcagaaa
      181 gtttaatacc aggaacttca ttgtgtagaa aaacagcgtt attataactt gttattggca
      241 taattccgat aaaaaagggt tgttcaactg ttttagtaag atcagctaat tcctttaaca
      301 cagagtgatc aaatattggt tgtgtcataa aataatcagc accagaagct acttttttct
      361 caatcaattt tacggttcga gtaaggtttt tcacattagg attaaaggca gcagcaactg
      421 taaaatctgt tggacgtctc aagctagcac cactataaga taaaccttga ttaagttgtt
      481 taatcagaga aaggagttta aaggaagtca catcataaac actagtcgct cctgggaaat
      541 caccaagttt agtcggatct cctgttatgg ctaaaatatg gttaaaacct aataattcca
      601 tgcccaacag tctagactgt aaaccaatta aattatggtc acgacaagct atatgtagta
      661 aaaagggtgt gctaatttca tctttaagca agctagctat acttaaatta caaatacgtg
      721 tattagatag tgaattatct gctaaagtaa ttgctgcaat acctttttgg tcaatcgctc
      781 gtatagcctt ttgaaactga acgatatcta aatgctttgg aggatctaat tctgcgataa
      841 ttgttacttc tttttttacc ttatccacta gagtatctgt tcgtctgata cgcctaacaa
      901 aatcttttac aggtattata ggagtaacaa cctttcgttc aataggtttc aatcctctaa
      961 tagatcgttt cacagctcta atatgatctg gagttgtccc acaacaacca ccaataagcc
     1021 taactccttc tgctacgaga agttctgcag attttccaaa atactctgaa ttctgactaa
     1081 actggtattg actattctca ccatctaaag ataactgact tgcattagga taaactgaca
     1141 aataagactg ggcaaataaa ggaacttgct tcaatgattg gatcatatga tagggtccaa
     1201 ggtgacaatt taagccaatc acatctgcac ctagcataac taattggctg agcgcctcta
     1261 caataggctt tccatttgat gttacccctg cttcatgaag agaaatatta gtaataattg
     1321 gtagatcagt catctccctc acaattttta aaactgtcgt tatctcctca atatcataat
     1381 aagtttcaaa caataatccg tctagctgtc ctgtcgccaa taaagcctca acttgttcca
     1441 aagtttcttc tacaatatta tccaatgtta aatcgcattg tctttgagaa cgaaaacctc
     1501 caacagtacc taaaataaat gtttctttac ccaaagtcgc ttgatgagct atattaactg
     1561 ctgcttgatt gatagagaca acctgatctt ccaaaccgta gttctttaat cgatgacgtt
     1621 gagccccata ggtattcgtc tgaataacat ctgcacccgc ttcaatataa gcttgatgaa
     1681 tggctagaac cttttctgga tgtgttacat tgtaggattc atgacaagta tctagcccat
     1741 aagtataaag gagagttccc attgcaccgt cagctactag aatatctgtt tttaatttct
     1801 ctaaaaactt ggacatcttc tttactcctc cctcatggca atcctttact gattaaccta
     1861 ttaattatct aattgctgac gaacctcttt ggtagctgaa acaagaacct ccaaggcagc
     1921 aattgtttct gcttcacggc gtgtttttaa gccacaatca gggtttaccc aaaattgctc
     1981 ttttgataga cattttagtg atcgttgaat attgacaata atttcttcct tagtaggtat
     2041 gcgaggggaa tgaatatcat aaacacccag gccaattcct agaggataaa ctgctgtttc
     2101 aaaactttca atgatgtccc catgacttct actcgtttca atagaaataa catctgcatc
     2161 tagtgcacgg atagaatcaa taatttcgtc aaattttgaa taacacatat gtgtatgaat
     2221 ttgtgtctca tctttcacag atgaagttgc aattttaaag gccgcaacag catcatctaa
     2281 gtaagcctgt tgcttttgct ggcgtaaggg taaaccttct cgaagggctg cttcatccac
     2341 ttgtataata gcaatacctg attgttccaa aagttgaatc tcatctttta tagccaaagc
     2401 aatttgatta aaaagatcag atcggctaat atcatctcgc tcaaatgacc agttcgttat
     2461 agtaatagga cctgtcaaca tgcctttaac aggtttctta gtcaaacttt gggcataaac
     2521 cgtttcttca aggcttaagg gttgaatatg tttgacatca ccataaatga taggtggttt
     2581 gaccgccctt gaaccataag actgtaccca gcctaatttg gttgtcgtaa aaccagctaa
     2641 cttttgacca aaaaattcaa ccatatcaac acgctcaaat tcgccatgta ctaacacatc
     2701 aagatcaaga tcttcttgaa tagcaatcca tcttctaatt tcagttttta tgaaatcttt
     2761 ataatctgag tcagatagat ttcctctttt ccaagcaagg cgtttcttcc taatttcagg
     2821 tgattgagga aaggatccaa tagttgttgt tggtaaaatc ggtaactgta atttttcagc
     2881 ttgcaaagct tggcgtaatt tatacggact tcgttcaagc tttacttctg ctaatgattc
     2941 caatgctaca tgacgaaaat cagcagcttg aagagctgaa aaacgagata aagcttcgtg
     3001 atatcccttt gttttattac catcaaaagc actagctaat agttctaatt cctttagttt
     3061 ttcatcagca aatgataagc cattctttaa cacagggtct aaatgtgttt cgtatttagt
     3121 cgtcaccggt acatgtaaaa gacttgaaga aggttgaaga gtcaatcgtt taacaaaggg
     3181 acctatctct tccaataaag ccaacgtttc ttctaaattt actgcccaga tatttcgacc
     3241 attaacaatt cctgcaaata attctttttc gcggaataga ccttgcttaa cagcagctaa
     3301 attttccgcc ctaccatgta taaaatcaag gccaaatccc gttactggca gcttagagag
     3361 tttatctaaa cagactgctg attcaaagta ggtttgaaag atgaagtggg cttgtggtac
     3421 ttctcttgca aaaaaatcat aaacagactt agctatatct actaaaagtt caccttcatc
     3481 agttacaaat atcggctcat caatctgaat ataggtggcg cctgcatcta tcagatcttg
     3541 aaagacctgc ttataaagtg gtaataaccg ctgaacagtc gcttcaaagt cgacaattcc
     3601 tgatgataag gaaacatagg ttattggacc agtgataacc ggctttgctt tatcaccaac
     3661 tacttcccta gcttctagat aaagatcaag taagtaatta ttctgcaagt gaggtttagt
     3721 ctcaacctcc cattctggga ctatgtagtg gtagttggta ttaaaccact ttttcataga
     3781 tgatgcgaca ttatctttat cacctcttgc aatagcaaaa taaaggtcta aattcctctc
     3841 atactcatcg aaacgctttg ggattacatt gaattgaaat gacaaatcca aaacatgatc
     3901 ataacaagaa aaatccccca ctggaataag gtcaatacct gcctcttttt gtttctttaa
     3961 atgattgata cgtaattgtt ttagttgttt ttctaaatct ttttgttcaa gattccctgc
     4021 ccagaaagct tcgatcgctt gcttccattc gcgctgttca ccaagacgtg gataccctaa
     4081 atttgaaact tttaccataa tttcctccat caagttatag ctaataacta acccttttag
     4141 ttttatgaat tattttagtc catgaaaact ataatatcaa tagcttttaa tattttatat
     4201 ttttagcgat ttatatagt
//
LOCUS       DBJYSH010000130         4192 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.130, whole genome shotgun sequence.
ACCESSION   DBJYSH010000130 DBJYSH010000000
VERSION     DBJYSH010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 4192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4192
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.130"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            109..1353
                     /locus_tag="ACUBV3_001823"
     CDS             109..1353
                     /locus_tag="ACUBV3_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HHU3848300.1"
                     /translation="MINSLLLKSLHSTLKLSITVCDRSFTVLTEYKSDKTVSLYYDHH
                     LILNEFSKTKENFLFHYGFMGELFLALRTDNHYVIIGPWRSNAIDPLLFKRKMNEAHI
                     ENDEQNYFFEKLVQLPFFPLSQIRELLILVNYCLTGKIEDLLSAPLHHYTKGWSNAFD
                     LNKIKQLSVENSNTYTYQYQYETRILQAVKSGKEELLRETVSQLSNAVSATLSGDELR
                     SEKNYSIMIYDRLAQGAVHSGLDIETAYQSRDRFVTETEQAQSLHAVLKLRDTAILFY
                     TQQVSGIKQKLVSQYSQTVIGVIQYLENNLNRAIKTAEIARQFHMSESKLRKLFKQEK
                     HMTILQYFLHLKIEVAKQLLEEGKTLAHIAELLQFSTSANFSRTFKRVVGISPLKYAQ
                     QNNLASQQTSKGQTQKDPEESH"
     gene            1671..2294
                     /locus_tag="ACUBV3_001824"
     CDS             1671..2294
                     /locus_tag="ACUBV3_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YczE/YyaS/YitT family protein"
                     /protein_id="HHU3848301.1"
                     /translation="MKFLRFMIGINIMALAVALSKVSQLGTSPIASIPNVLSEVWKQS
                     IGLYMLLFMLFLIILQIIIVGHQINKVKLFNICIQMFPGAIFGYFINCYVNLLKILPP
                     FDNYFLKISLLLISIVILALGVVIEVKADFILMPGEGLPQTIAKHFHLPFAKVKLWSD
                     LSMVFTALILSFLLSRPFLGIREGTLLATVFTAPCVSMITYSLDKLK"
     gene            complement(2862..4163)
                     /locus_tag="ACUBV3_001825"
     CDS             complement(2862..4163)
                     /locus_tag="ACUBV3_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3848302.1"
                     /translation="MVNQVNNLTEFIEAIKIENNDIYVASSILIPYSVTLSAGVSIHG
                     SRDKGTMLSFPNSDGIALTKNNKLSDLIIQSISNQRAIYISSSDEDLSTMTLEKLTVT
                     GQVQLLTRAPNKTMELIIDDLDIPFSDSRNRSEKPLKYGVVVQQGALTIFNYNQDSAS
                     TISADIKNVSIGRKNAPVLGSGVFIAGFNETGGSVEVKELVTKDIYSNGMIPFGQPNM
                     ITGGIFILTGAHAQSIHSQGTVTTYGVNDMVLDVWGEVDSWITEKPIESFGTSGIGFV
                     NFGTVHRFQANDAVVTYGSGARGFNQYDGTIDFASFKSITTYGDGSIGMQFSKPVGEI
                     IIEDSITTSGDVGDSLVKGEIQSLKAIALSLQPGGEIENLSVGKNIATKGKHVHTIEI
                     KQDAFLHDFSIGGEIKQEGNDNPKVIIEETLSADIKALLDK"
BASE COUNT         1336 a          744 c          665 g         1447 t
ORIGIN      
        1 aatttttttg atcatttttg gtatttttaa cttttagagg tgatatctat tgactttctt
       61 agaatctttt ttagaattat tactataaat caaaaagagg aggtgctcat cataaactct
      121 ttactgttaa aatcattaca tagcacttta aaattatcta ttaccgtttg tgacagatcc
      181 tttacagttc taacagagta taaatcagat aaaaccgttt ctctttatta tgaccatcat
      241 cttatcttaa atgagtttag taaaaccaaa gagaatttct tatttcacta tgggtttatg
      301 ggagaattgt tccttgctct tcgcacagac aatcactacg tcattattgg tccttggagg
      361 agtaatgcca ttgatccttt actctttaaa aggaaaatga acgaagctca tattgagaac
      421 gatgaacaaa attatttctt tgagaagttg gttcaacttc ctttcttccc cctcagtcaa
      481 attagagagc tacttatttt agttaactat tgtttaactg ggaaaattga agatttgttg
      541 tcagcccctt tacatcacta tacaaaaggg tggagcaatg cttttgattt aaataaaatc
      601 aaacagttgt ctgtcgaaaa tagtaatacc tatacttatc aataccagta tgaaactagg
      661 attttacaag ctgttaaatc tgggaaggaa gagcttttaa gagaaactgt aagtcaatta
      721 agcaatgccg tttctgctac gttaagtggc gatgaattac ggtccgaaaa gaattactct
      781 attatgattt atgatcgttt agctcaagga gctgttcact ctggccttga tattgagaca
      841 gcctatcaat cgcgagatcg ctttgttaca gaaacagaac aagctcaatc attacacgct
      901 gttttgaagt tacgcgatac agctattcta ttttacactc aacaagtgtc ggggattaaa
      961 caaaaactag tatcacaata ttctcaaaca gttattgggg ttattcaata tcttgaaaat
     1021 aacctcaatc gagctattaa gactgcggaa atagccagac agtttcacat gagtgaatct
     1081 aaattacgaa agttatttaa acaagaaaag cacatgacca ttctacaata ttttcttcat
     1141 ttaaaaattg aagtggctaa acagttatta gaggaaggaa aaacattggc tcacattgcg
     1201 gaattactcc aattctcaac atcagctaat ttttcacgta ccttcaaaag agtggttggg
     1261 atcagtccat taaagtatgc gcagcaaaat aatttggcga gtcagcagac cagtaaagga
     1321 cagactcaaa aagatccaga ggagagccac tgatgacagg ctcctctctg gatctttttc
     1381 tatataaaaa aactctataa ctactgccaa tggtatcacc cactacaatt gacaatggca
     1441 aaataaatgg aaacttacta ttatcccatt caatgatata cctaatgtta taaaatattc
     1501 atttcaacta attcgatatg ctacatagtt ggtttaaact aatttctgta ttatggttta
     1561 cgtaaactta ttaaaggtat ataattaagt tgttgtttgc tttcaattac ctctcaatcg
     1621 tcattagatt tgataaatga atgtttctca tatgggaaag gatgattaag ttgaaatttt
     1681 tacgatttat gattggtatc aatattatgg ctttagctgt tgccttatcc aaagtctcac
     1741 aattgggaac ttctcctatt gcctctattc ccaatgttct atcagaagta tggaaacaat
     1801 cgatagggtt atacatgctg ttgtttatgc tttttttgat tattctacaa ataataattg
     1861 ttggtcatca aataaataaa gttaagcttt tcaatatttg tattcaaatg ttccctggcg
     1921 ctatatttgg atattttatc aattgttatg ttaacctttt gaaaatactt cctccatttg
     1981 acaattattt tttaaagatt agcttactat tgattagtat agttatttta gcgctgggtg
     2041 tagttattga agttaaagca gattttattt taatgcctgg agaaggatta cctcaaacaa
     2101 tagcaaagca ttttcactta cctttcgcaa aagtcaaact gtggtcagat ttatccatgg
     2161 tgttcacggc tttaatcctt tcgtttctat tatcgagacc ctttttggga atacgcgaag
     2221 ggacacttct tgcaacagta ttcacagctc cttgtgtgtc aatgattaca tatagccttg
     2281 acaaattaaa gtagtatgct actaacaatt catagattta aacctttcta gtatgatgtt
     2341 tgggttaaaa aaataatcaa caaaatgcta ctttactaat tattctactg attccaaaaa
     2401 gttggacaat aattagtgaa agaactttgg acatatcaag caataataga caaaaagtta
     2461 agctaattaa agactatttc tttcagactc tattcctctt gtaatatctt attttccaag
     2521 ttttttctca aattgagcat gaccatgaca aagagcgctc ccatgtcttg ggaacgctct
     2581 ttcttcatct atttcatatt cttgaaacca acgaaaccat ttggatgaat tcctaaagct
     2641 tctccaacga tttttacaga ctactccgcc tcaagaaaac tttaacaaca gctaatttaa
     2701 acgccttatc aaatgttttc ttgacttgat tattttcctc gtttccactt aattttgtgt
     2761 ccacattatt taaccaggtc caatcccaaa agttagattt tctgtctaac ttttgagatg
     2821 caagtcatta tttagttggg ggaatatttt ttaagaacta gttatttatc tagtaaagct
     2881 tttatatctg cagaaagtgt ttcttctatg ataacctttg gattgtcatt tccctcttgt
     2941 ttgatctctc cgcctattga aaaatcatga agaaaagcat cttgcttaat ttcaatggtg
     3001 tggacatgtt tacctttagt tgcaatgttt ttaccaacac taagattttc tatctcaccg
     3061 ccaggttgta gggataaagc aattgcctta agggattgaa tttcaccttt tactaaggaa
     3121 tctccaacat caccagatgt agtaatagaa tcttcaataa taatctctcc cactggtttt
     3181 gaaaactgca ttccaattga accatctccg taagtagtaa ttgacttaaa tgaggcaaaa
     3241 tctattgtac catcatattg attaaacccc cgtgctcctg agccgtaagt cacaactgca
     3301 tcattggctt gaaaacgatg cactgtccca aaattgacaa atccaatgcc agaagttcca
     3361 aagctttcaa taggtttttc tgtaatccag ctatccactt caccccatac atctaagacc
     3421 atatcgttta caccgtaggt ggtaactgtt ccttgagagt gaattgattg agcatgagcg
     3481 ccggttaaaa tgaatattcc tcctgtaatc atattaggct gaccaaaagg aatcatacca
     3541 tttgaataaa tatctttagt tactaattcc ttaacttcta cagaaccacc tgtttcatta
     3601 aaacctgcaa taaaaacacc tgatcctaat acaggggcat tcttacgacc aattgaaacg
     3661 tttttaatat cggctgatat ggttgatgca ctgtcttgat tataattaaa gatagttaaa
     3721 gccccttgtt ggacgacgac tccatatttt agtggctttt cggaacgatt tctggagtcg
     3781 ctaaatggga tgtctaagtc atcaataatc agttccatag ttttatttgg tgcgcgtgtt
     3841 aatagttgaa cctgaccagt tactgtgagc ttctctaaag tcattgtaga taaatcttcg
     3901 tcacttgatg aaatgtaaat cgctctttgg ttagagatgc tttgaataat taagtctgaa
     3961 agcttattat tttttgttaa agcaatccca tcactatttg gaaaacttaa catggttcct
     4021 ttatctctag aaccatgaat acttactccc gctgaaagtg tcacagaata aggaataagg
     4081 atagatgaag cgacataaat atcattgttt tcaattttta ttgcttcgat gaattcagtt
     4141 aaattattaa cttgatttac cattgtaata cctccaacta tttatggtaa ta
//
LOCUS       DBJYSH010000131         4168 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.131, whole genome shotgun sequence.
ACCESSION   DBJYSH010000131 DBJYSH010000000
VERSION     DBJYSH010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 4168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4168
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.131"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            14..556
                     /locus_tag="ACUBV3_001826"
     CDS             14..556
                     /locus_tag="ACUBV3_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HHU3848303.1"
                     /translation="MTETYIKNTTNNSGTTAVRGELTFEDKVIEKIVGIAIEHVDGLL
                     AVNGGFFSNLKNSVVNSDSVTDGVNVEVGKKQVAVDLDIVAEYQKHVPTIFADIKKVV
                     EAEVKRMTDLEVVEVNVNVVDIKTRAQHEEDSVTLQDRVTSAAQATGEFASNQVSNVK
                     SAVGNGVDKVEDMKSEPRVQ"
     gene            603..800
                     /locus_tag="ACUBV3_001827"
     CDS             603..800
                     /locus_tag="ACUBV3_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HHU3848304.1"
                     /translation="MSEEKFDAKVDKVSGSVKESVGKLTGDKEVESEGKVDKLKGHAK
                     EKLADIKDTIKGASESFKKKD"
     gene            822..1373
                     /locus_tag="ACUBV3_001828"
     CDS             822..1373
                     /locus_tag="ACUBV3_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HHU3848305.1"
                     /translation="MENQKQDIKTSVTYEEKVIAKIVGHALESVDGLLAVSGGFFSNL
                     KNSVVNSDSVTDGVNVEVGTKEVAVDLDIVVEYGKDIPAIVESIKAIVSQNVEVMTHL
                     KVVELNANVVDVKTKAEHEADSVTVQDRVSDAAQATGNFASEQAGKAKAAISSGAEKT
                     KEAVSNGTEAAKEKISEARTSES"
     gene            1537..2178
                     /locus_tag="ACUBV3_001829"
     CDS             1537..2178
                     /locus_tag="ACUBV3_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000253259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HHU3848306.1"
                     /translation="MVSEQSEIVTSKYQKIAVAVAQRIANGDYEVGEKLKSRTTIAST
                     FNVSPETARKGLNILADLQILTLKHGSGAIILSKEKAIAFLNQYETSHSVAILKGKIR
                     DNIKAQQQEMEELATLVDDFLLQTRAVSKQYPLAPYEIIVSEDSEHLGKSIGELNVWH
                     QTGATIVAIEHEGKFIVSPGPFSVIEQGDHIFFVGDEDVYARMKTYFNLRMGL"
     gene            2183..2947
                     /locus_tag="ACUBV3_001830"
     CDS             2183..2947
                     /locus_tag="ACUBV3_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000348145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HHU3848307.1"
                     /translation="MDFVFDIDGTLCFDGMSLSKEIQGILERAQIDYGHRVTFATARS
                     YRDTIGILGDKLSLSKIIGLNGATLHENGHLVDSYYLQSDFFSTIISYCHRYQIPYFV
                     DEVFNYATYQASKIPFIAYVDPQKRGELLEVSKIEKPIKMVLYFGDQLRRADQMLAKL
                     NRFGLSSHFFHEFEKCLYINPIAVDKGKATKKLFGNRFIAFGNDKNDISMFDAAHYSV
                     QVGDFDELTPYANLRVSRESVHEGITMLFEKYKGIT"
     gene            2947..3606
                     /locus_tag="ACUBV3_001831"
     CDS             2947..3606
                     /locus_tag="ACUBV3_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HHU3848308.1"
                     /translation="MDLFLSLERKFKAASDKEVSKQQEAYLRHHFKCYGIKSPERRML
                     YKELIKAAKRQAKIDWQLLDKCWQSDYREYHHFVLDYLLAMSQFLTYNDCSRLEFYAR
                     HQQWWDSIDVLTKIFGNLSLKDDKVMNLLSEWSLDQDFWMRRLAIEHQLGFKEKTNTD
                     ILSLFILRNTGSQEFFINKAIGWALRDYSKYNKVWVKDFISNHYDELSTLSIREGSKY
                     L"
     gene            3679..>4168
                     /gene="tpx"
                     /locus_tag="ACUBV3_001832"
     CDS             3679..>4168
                     /gene="tpx"
                     /locus_tag="ACUBV3_001832"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005591554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HHU3848309.1"
                     /translation="MTTFLGNPVTFTGKQLQVGDIAKDFLLIATDLSQKSLKDFEGKK
                     KVISVVPSIDTGICSKQTRTFNEELSELDNTVVITVSMDLPFAQKRWCSAKGLDNVIL
                     LSDFYDHSFGQEYALLMNEWHLLTRAVLILDEHNKVTYTEYVDNVNSDVDYEAAINAA
                     KIL"
BASE COUNT         1410 a          576 c          869 g         1313 t
ORIGIN      
        1 aagaggaact attatgactg aaacttacat caaaaacaca actaacaatt ctggcacgac
       61 agctgttcgt ggagaattaa ctttcgaaga taaagtgatt gaaaaaattg ttggaattgc
      121 gattgagcat gtagatggtc ttttggctgt aaatggtggt tttttctcaa atttaaaaaa
      181 tagcgttgta aattctgatt cagttacaga tggcgtaaat gttgaagtgg gtaaaaagca
      241 agttgctgtt gatttggata ttgttgctga ataccaaaaa catgttccaa ctatctttgc
      301 cgatatcaaa aaagttgttg aagctgaagt gaaacgtatg acagaccttg aagtggttga
      361 agttaatgtt aatgttgttg atattaaaac tcgtgctcaa catgaagaag attcagtaac
      421 tcttcaagat cgtgtaacaa gtgcagcaca agcaacaggt gaatttgctt cgaatcaagt
      481 ttcaaacgtt aaaagtgccg taggtaacgg tgttgataaa gtggaagaca tgaagtctga
      541 accacgtgta caataattga tttaagtata gtttaaagta ttgttattag taggaggcta
      601 ttatgtctga agaaaaattt gatgcaaaag ttgataaggt ttctggttca gtaaaagaat
      661 ctgttgggaa acttactggc gataaagaag ttgaatctga aggtaaagtc gataaattga
      721 aaggtcatgc aaaagaaaaa cttgctgaca ttaaagatac cattaagggt gcttcagaga
      781 gctttaagaa aaaggactag aaggtttgag gtcatttatt tatggaaaat caaaaacaag
      841 atattaaaac ttcagtaact tatgaagaaa aggtaattgc taaaattgtt ggacatgctt
      901 tagaatcagt agacggactc ttggctgtga gtggaggttt cttctctaat ctaaaaaata
      961 gcgttgtaaa ttctgactca gtaactgatg gtgtgaatgt tgaagttggt actaaagaag
     1021 ttgcagttga tttagacatc gttgttgaat acggcaagga tatcccagct atcgttgaaa
     1081 gcattaaagc gattgtcagt caaaacgttg aagtgatgac ccaccttaaa gttgttgaat
     1141 tgaacgcaaa tgtcgtagat gttaagacta aagctgaaca tgaagctgac tcagtgacag
     1201 tacaagatcg tgtatctgat gcagcacaag caacaggaaa ctttgcttct gaacaagctg
     1261 gcaaagctaa agcagcaatt tcttctggag cagagaaaac aaaagaagca gtatcaaatg
     1321 gtactgaggc tgcaaaagaa aaaatctcag aagctcgtac tagtgaatct taattcagta
     1381 aactactttt tatgataaga cactcggagc tgggagacca gctcctttta tatgatattt
     1441 actaaagaca ttagatatta acaaaaaaga tatatttgca atattttgct aattgtggtt
     1501 taatatgtat gtttttatta ggataaggag ggttggatgg tttctgaaca atctgaaatt
     1561 gtaacatcta aatatcaaaa aatagcagta gcggttgcac aaagaatcgc aaatggagat
     1621 tatgaagttg gagagaagtt gaaatctaga acaactattg cttcaacatt caacgtatca
     1681 ccagaaacag cacgtaaggg tcttaatatt ttagcagatt tacagatttt aactttaaag
     1741 catggcagtg gagccattat tctttctaaa gaaaaagcaa ttgcattctt aaaccaatat
     1801 gaaacctctc attcagttgc tattttgaaa ggtaaaatcc gtgataatat taaggctcag
     1861 caacaagaaa tggaagagtt agctacttta gttgatgatt ttttgcttca aacacgtgcg
     1921 gttagtaaac aatacccact agcaccatat gaaattattg tcagtgaaga ttctgaacac
     1981 cttggtaagt caataggaga attaaatgta tggcatcaaa ctggtgcgac tattgtggct
     2041 attgaacacg aagggaaatt tattgtttct ccagggcctt tctcagttat agagcaagga
     2101 gatcatatct tttttgttgg tgatgaagat gtttatgcac gtatgaaaac ctattttaac
     2161 ttaaggatgg ggctttgaga gaatggattt tgtctttgat atagatggaa ctctttgttt
     2221 tgatggtatg agtctatcta aagaaataca aggaatacta gagagagccc agattgatta
     2281 tggtcataga gtcacttttg ctacggcgcg atcttataga gacactatag gtatccttgg
     2341 cgacaagcta tctttatcaa aaattattgg tttaaatggt gcgactttac atgaaaatgg
     2401 tcacttagtg gatagttact atttacaaag tgactttttt agtactatta tttcatattg
     2461 tcatcgctat cagattccat attttgttga tgaggtcttt aattacgcta cttatcaggc
     2521 ttctaaaatt ccctttattg cttatgttga tcctcaaaaa aggggagagc ttctagaagt
     2581 ttctaaaatt gagaaaccga taaaaatggt gttgtatttt ggtgatcagc tgagaagggc
     2641 agaccaaatg ttagcgaaat taaatcgttt tggacttagt agtcactttt tccacgaatt
     2701 tgaaaaatgt ttgtacataa atcccattgc agttgacaag ggaaaagcca ctaagaaatt
     2761 atttgggaat cgttttatag cttttggcaa tgataaaaat gatatttcta tgtttgatgc
     2821 tgcgcattat agtgttcaag taggggattt tgatgaactg actccttacg ctaatttacg
     2881 tgtatcaaga gaatctgtac atgaaggaat tacaatgctt tttgaaaaat acaaggggat
     2941 tacgtaatgg atttattcct aagtttagag aggaaattta aagctgcaag tgacaaagaa
     3001 gtttctaagc aacaggaggc ttatttacgc catcatttta agtgctatgg tattaaatca
     3061 cctgagcgac gtatgctcta caaagagctt ataaaggccg ctaaaagaca agcaaaaatt
     3121 gattggcaat tgcttgataa atgttggcaa agtgactatc gtgaatatca tcattttgta
     3181 cttgattatc tattagctat gagtcaattt ctcacctata atgattgctc tagactagag
     3241 ttttacgctc gtcatcaaca gtggtgggat agtattgatg ttctgactaa gatttttggg
     3301 aacttatcct taaaagatga caaggttatg aatttactgt cagaatggtc acttgatcaa
     3361 gatttttgga tgaggcgact agctattgag catcaactag gatttaaaga aaaaacaaat
     3421 actgatattt tgtcactttt tattttaaga aatacaggaa gtcaagaatt ttttatcaat
     3481 aaggcgattg gctgggcgct tagggattat tcaaagtata ataaagtatg ggtaaaggac
     3541 tttatcagta atcattatga tgagttgtcg actttgagca tacgcgaagg aagtaagtac
     3601 ctctaattat cagttcaatt attcgaaaaa gaagaagctt catagtaaaa taaaggtaat
     3661 tgataaggag ataaacttat gacgacattt cttggaaatc ctgttacttt tacaggaaaa
     3721 cagctacaag ttggggatat tgcaaaagat tttttactaa tagctacaga cttatctcaa
     3781 aaatctttaa aagattttga agggaaaaag aaagttatta gcgttgttcc ttctattgat
     3841 acaggtattt gttcaaaaca aacacgtact tttaatgagg aattatcaga gctagataac
     3901 acagtggtta ttactgtttc tatggacctt ccatttgctc agaagcgttg gtgcagcgct
     3961 aaaggtcttg ataacgtgat tttattatca gatttttatg atcattcgtt tggtcaagag
     4021 tacgctcttt tgatgaatga gtggcatttg ttaacgcgtg cagttcttat tttggatgag
     4081 cataataagg tgacttacac cgaatacgtt gacaatgtta attcagatgt tgactacgaa
     4141 gcggcgatta atgctgcaaa gatattac
//
LOCUS       DBJYSH010000132         4073 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.132, whole genome shotgun sequence.
ACCESSION   DBJYSH010000132 DBJYSH010000000
VERSION     DBJYSH010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 4073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4073
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.132"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            548..4000
                     /gene="scpA/B"
                     /locus_tag="ACUBV3_001833"
     CDS             548..4000
                     /gene="scpA/B"
                     /locus_tag="ACUBV3_001833"
                     /EC_number="3.4.21.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021340616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C5a peptidase ScpA/B"
                     /protein_id="HHU3848310.1"
                     /translation="MRKKQKLPFDKLAIALISTSILLNAQSDIKANTVTEDTPATEQA
                     VEPPQPIAVSEESRSSKETKTSQTPSDVGETVADDANDLAPQAPAKTADTPATSKATI
                     RDLNDPSHVKTLQEKAGKGAGTVVAVIDAGFDKNHEAWRLTDKTKARYQSKENLEKAK
                     KEHGITYGEWINDKVAYYHDYSKDGKNAVDQEHGTHVSGILSGNAPSEMKEPYRLEGA
                     MPEAQLLLMRVEIVNGLADYARNYAQAIRDAVNLGAKVINMSFGNAALAYANLPDETK
                     KAFDYAKSKGVSIVTSAGNDSSFGGKPRLPLADHPDYGVVGTPAAADSTLTVASYSPD
                     KQLTETATVKTDDHQDKEMPVISTNRFEPNKAYDYAYANRGTKEDDFKDVEGKIALIE
                     RGDIDFKDKIANAKKAGAVGVLIYDNQDKGFPIELPNVDQMPAAFISRRDGLLLKDNP
                     PKTITFNATPKVLPTASGTKLSRFSSWGLTADGNIKPDIAAPGQDILSSVANNKYAKL
                     SGTSMSAPLVAGIMGLLQKQYETQYPDMTPSERLDLAKKVLMSSATALYDEDEKAYFS
                     PRQQGAGAVDAKKASAATMYVTDKDNTSSKVHLNNVSDKFEVTVTVHNKSDKPQELYY
                     QVTVQTDKVDGKHFALAPKALYETSWQKITIPANSSKQVTVPIDASRFSKDLLAQMKN
                     GYFLEGFVRFKQDPTKEELMSIPYIGFRGDFGNLSALEKPIYDSKDGSSYYHEANSDA
                     KDQLDGDGLQFYALKNNFTALTTESNPWTIIKAVKEGVENIEDIESSEITETIFAGTF
                     AKQDDDSHYYIHRHANGKPYAAISPNGDGNRDYVQFQGTFLRNAKNLVAEVLDKEGNV
                     VWTSEVTEQVVKNYNNDLASTLGSTRFEKTRWDGKDKDGKVVANGTYTYRVRYTPISS
                     GAKEQHTDFDVIVDNTTPEVATSATFSTEDSRLTLASKPKTSQPVYRERIAYTYMDED
                     LPTTEYISPNEDGTFTLPEEAETMEGATVPLKMSDFTYVVEDMAGNITYTPVTKLLEG
                     HSNKPEQDGSDQAPDKKPEAKPEQDGSGQTPDKKKETKPEKDSSGQTPGKTPQKGQSS
                     RTLEKRSSKRALATKASTRDQLPTTNDKDTNRLHLLKLVMTTFFLGLAAHIFKTKRQK
                     ETKK"
BASE COUNT         1412 a          835 c          803 g         1023 t
ORIGIN      
        1 aggagcgcga actcctacac caaaccgatt aagacaaata ccacttgctt aatcagtcga
       61 accttattgt agcataacta aaacaatacc gtcaagactg aacaagcaaa aaatacttgt
      121 tttggtcttt ttaagaataa ttcaagtttt cttaaattta tgaggtatcg actaatctca
      181 aaatttcaga cacatacctg agtgaacatc aagagagaac cgatcggttc tctcttgatg
      241 tctaaaagaa tgaggttaag gaggtcacaa actaaacaac tcttaaaaag atgaccttta
      301 ctaataatcg tctttgtttt ataataaaga tgttagtaat ataattgata aatgagatac
      361 atttaatcat tatggcaaaa gcaagaaaaa tagctgtgtc atatgcaaat aacccctgtt
      421 tgctctttaa aaaagacgtt atccttattt ctctacacac agacggatag ctaggagaga
      481 atcgtttgat tctctctttt cttaatggtc ataaagacaa agtctctcat catgaaagga
      541 cgacacattg cgtaaaaaac aaaaactacc atttgataaa cttgccattg cgcttatatc
      601 tacgagcatc ttgctcaatg cacaatcaga cattaaagca aatactgtga cagaagacac
      661 tcctgctacc gaacaagccg tagaaccccc acaaccaata gcagtttctg aggaatcacg
      721 atcatcaaag gaaactaaaa cctcacaaac tcctagtgat gtaggagaaa cagtagcaga
      781 tgacgctaat gatctagccc ctcaagctcc tgctaaaact gctgatacac cagcaacctc
      841 aaaagcgact attagggatt tgaacgaccc ttctcatgtc aaaaccctgc aggaaaaagc
      901 aggcaaggga gctgggaccg ttgttgcagt gattgatgct ggttttgata aaaatcatga
      961 agcgtggcgc ttaacagaca aaactaaagc acgttaccaa tcaaaagaaa atcttgaaaa
     1021 agctaaaaaa gagcacggta ttacctatgg cgagtggatc aatgataagg ttgcttatta
     1081 ccacgactat agtaaagatg gtaaaaacgc tgttgatcaa gaacacggca cacacgtgtc
     1141 agggatcttg tcaggaaatg ctccatctga aatgaaagaa ccttaccgcc tagaaggtgc
     1201 gatgcctgag gctcaattgc ttttgatgcg tgtcgaaatt gtaaatggac tagcagacta
     1261 tgctcgtaac tacgctcaag ctatcagaga tgctgtcaac ttgggagcta aggtgattaa
     1321 tatgagcttt ggtaatgctg cactagctta cgccaacctt ccagacgaaa ccaaaaaagc
     1381 ctttgactat gccaaatcaa aaggtgttag cattgtgacc tcagctggta atgatagtag
     1441 ctttgggggc aagccccgtc tacctctagc agatcatcct gattatgggg tggttgggac
     1501 acctgcagcg gcagattcaa cattgacagt tgcttcttac agcccagata aacagctcac
     1561 tgaaactgct acggtcaaaa cagacgatca tcaagataaa gaaatgcctg ttatttcaac
     1621 aaaccgtttt gagccaaaca aggcttacga ctatgcttat gctaatcgtg gtacgaaaga
     1681 ggatgatttt aaggatgtcg aaggtaagat tgcccttatt gaacgtggcg atattgattt
     1741 caaagataag attgcaaacg ctaaaaaagc tggtgctgta ggggtcttga tctatgacaa
     1801 tcaagacaag ggcttcccga ttgaattgcc aaatgttgac cagatgcctg cggcctttat
     1861 cagtcgaaga gacggtctct tattaaaaga caatccccca aaaaccatta ccttcaatgc
     1921 gacacctaag gtattgccaa cagcaagtgg caccaaacta agccgcttct caagctgggg
     1981 tctgacagct gacggcaata ttaagccgga tattgcagca cccggccaag atattttgtc
     2041 atcagtggct aacaacaagt atgccaaact ttctggaact agtatgtctg caccattggt
     2101 agcgggtatc atgggactgt tgcaaaagca atatgagaca cagtatcctg atatgacacc
     2161 atcagagcgt cttgatttag ctaagaaagt attgatgagc tcagcaactg ccctatatga
     2221 tgaagatgaa aaagcttatt tttctcctcg ccaacaggga gcaggagcag tcgatgctaa
     2281 aaaagcttca gcagcaacga tgtatgtaac agataaggac aatacctcaa gcaaggttca
     2341 cctgaacaat gtttctgata aatttgaagt aacagtaaca gttcacaaca aatctgataa
     2401 acctcaagag ttgtattacc aagtaactgt tcaaacagat aaagtagatg gaaaacactt
     2461 tgccttggct cctaaagcat tgtatgagac atcatggcaa aaaatcacaa ttccagccaa
     2521 tagcagcaaa caagtcaccg ttccaatcga tgctagtcga tttagcaagg acttgcttgc
     2581 ccaaatgaaa aatggctatt tcttagaagg ttttgttcgt ttcaaacaag atcctacaaa
     2641 agaagagctt atgagcattc catatattgg tttccgaggt gattttggca atctgtcagc
     2701 cttagaaaaa ccaatctatg atagcaaaga cggtagcagc tactatcatg aagcaaatag
     2761 tgatgccaaa gaccaattag atggtgatgg attacagttt tacgctctga aaaataactt
     2821 tacagcactt accacagagt ctaacccatg gacgattatt aaagctgtca aagaaggggt
     2881 tgaaaacata gaggatatcg aatcttcaga gatcacagaa accatttttg caggtacttt
     2941 tgcaaaacaa gacgatgata gccactacta tatccaccgt cacgctaatg gcaaaccata
     3001 tgctgcgatc tctccaaatg gggacggtaa cagagattat gtccaattcc aaggtacttt
     3061 cttgcgtaat gctaaaaacc ttgtggctga agtcttggac aaagaaggaa atgttgtttg
     3121 gacaagtgag gtaaccgagc aagttgttaa aaactacaac aatgacttgg caagcacact
     3181 tggttcaacc cgttttgaaa aaacgcgttg ggacggtaaa gataaagacg gcaaagttgt
     3241 tgctaacgga acctacacct atcgtgttcg ctacacgccg attagctcag gtgcaaaaga
     3301 acaacacact gattttgatg tgattgtaga caatacgaca cctgaagtcg caacatcggc
     3361 aacattctca acagaagata gtcgtttgac acttgcatct aaaccaaaaa ccagccaacc
     3421 ggtttaccgt gagcgtattg cttacactta tatggatgag gatctgccaa caacagagta
     3481 tatttctcca aatgaagatg gtacctttac tcttcctgaa gaggctgaaa caatggaagg
     3541 cgctactgtt ccattgaaaa tgtcagactt tacttatgtt gttgaagata tggctggtaa
     3601 catcacttat acaccagtga ctaagctatt ggagggccac tctaataagc cagaacaaga
     3661 cggttcagat caagcaccag acaaaaaacc agaagctaaa ccagaacaag acggttcagg
     3721 tcaaacacca gataaaaaaa aagaaactaa accagaaaaa gatagttcag gtcaaacacc
     3781 aggtaaaact cctcaaaaag gtcaatcttc tcgtactcta gagaaacgat cttctaagcg
     3841 tgctttagct acaaaagcat caacaagaga tcagttacca acgactaatg acaaggatac
     3901 aaatcgttta catctcctta agttagttat gaccactttc ttcttgggat tagcagctca
     3961 tatatttaaa acaaaacgcc aaaaagaaac taaaaaatag tattacttaa actgataaga
     4021 gcttatttaa tagagagaac caatttggtt ctctctattt ttttatagta cga
//
LOCUS       DBJYSH010000133         3998 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.133, whole genome shotgun sequence.
ACCESSION   DBJYSH010000133 DBJYSH010000000
VERSION     DBJYSH010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 3998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3998
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.133"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            32..547
                     /locus_tag="ACUBV3_001834"
     CDS             32..547
                     /locus_tag="ACUBV3_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3848311.1"
                     /translation="MKKLFKKAIAMCRDLPLNFVIVELSLYQREDGSSDNIKWISKRI
                     LEVTVTDQQWIYHLRMLNLPKLKPMSLASSDCPAREEKVSLISIEECQKFTSLVRDKN
                     PFHQGEAAIVPAALLLEKIMEVRKGKIKEIHLTFLSPVRVNQEFSVQVVDNTVIIFQH
                     QILCVRGTYEE"
     gene            537..1652
                     /locus_tag="ACUBV3_001835"
     CDS             537..1652
                     /locus_tag="ACUBV3_001835"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HHU3848312.1"
                     /translation="MKNRDVYIGFGLRTPIGIKGKQFKHYRPELLGAHLLNQIKKIEP
                     ESNIDSIICGNTVGTGGNIGRLMTLFSDYESYIPVQTIDMQCASSSSALFFGYLKISA
                     DINEKVLVGGIESSSLQPMRRYAKEDNRNGEYTVAQFSPDSYAETVMLEGAQRVCQKY
                     GFRREILDKLAFLSHKRALTAKQGGYLEEVILPMEGMRDQGVRKLKETFFQKLPRLME
                     NSPLLTIGNVCLMHDAAAFLTLQSQKTEFRIVHIVEVAGDPKLSPELVHTATEKLLTE
                     THTKISDYDAIEWNEPFAAIDALFNHYYPEEREKFNIFGGALAYGHPYACSGIINILH
                     LMQALKYKNKPMGLTAIAGAGGVGMAISIEYLGVKNA"
     gene            1645..2874
                     /locus_tag="ACUBV3_001836"
     CDS             1645..2874
                     /locus_tag="ACUBV3_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HHU3848313.1"
                     /translation="MLESLKTIVKTNSDKKLFDGDLEVSYGEFYNLVRQDMASQDNRK
                     HVISTHSLLNQLVRFVSKLCQKALPIICKPNLTHNEISRLEKEVQYAPQLADFGVLSS
                     GTTADAKLLWRSFASWSDFFSIQNAYFSVTSNSRLFIQGDFSFTGNLNLALSLLLLGG
                     TLVVTQKNSVKYWQTLWEKTGVTHLYLLPSYLKLVEQYSKETALDNKTIITSSQYVSD
                     SLLEGLYIKHPKVSVKIFYGASELNYVSWYDGRDIRDKPQYVGEIVPNVAVRIKEGRI
                     FVKTPYSICGLSSEYCAGDYGELIDGKLYLFGRGGDWCNQSGIKLYLPRLIEKIKTCP
                     YIKDAVAFTKESQSHGQESHCCIVLIENQMEQECLKWLSEHFEKKYGFKHYHIVSKIP
                     LMPSGKIDYQQLKRQLA"
     gene            2987..3619
                     /gene="nth"
                     /locus_tag="ACUBV3_001837"
     CDS             2987..3619
                     /gene="nth"
                     /locus_tag="ACUBV3_001837"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HHU3848314.1"
                     /translation="MLSKAKSRYIIREIIKLFPDAKPSLDFTNVFELLVAVMLSAQTT
                     DAAVNKVTPALFERFPNPLVLAQADPKEIEPYISKIGLYRNKARFLNQCAKQLIEHFD
                     GKVPQTRQELESLSGVGRKTANVVMSVGFGIPAFAVDTHVTRICKHHQICKQSASPLE
                     IEKRVMEVLPPEEWLAAHQSMIYFGRAICHPKNPKCDQYPQLYHFPDNLK"
     gene            complement(3620..>3998)
                     /locus_tag="ACUBV3_001838"
     CDS             complement(3620..>3998)
                     /locus_tag="ACUBV3_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001884316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HHU3848315.1"
                     /translation="YFISLCMSFILSYYDIKYQEYPIFLWILFTISTIVLTPITKVSI
                     VLCLFGILAEVVDINIGSGDFLYLATIGLSLPLHQMLFIIQIGAWLGIIYCLVMRKMK
                     KTIAFLPFLSIAYIIVTSYSLLF"
BASE COUNT         1358 a          634 c          783 g         1223 t
ORIGIN      
        1 atactggtct tatattttga cgggagataa gatgaaaaaa ctatttaaaa aagcgatagc
       61 tatgtgccga gatttgccgc ttaattttgt aattgttgaa cttagtttgt atcaaagaga
      121 ggatggctca tctgataata ttaaatggat ttctaagaga atccttgagg taacggtaac
      181 tgatcagcaa tggatttacc atttgaggat gcttaattta ccaaaactta agccaatgtc
      241 actagcatcc agtgactgtc ctgcaaggga agagaaggtt agtcttattt caatagaaga
      301 atgtcaaaaa ttcactagct tagtacgaga taaaaatcct tttcaccaag gggaagcagc
      361 tattgtgcct gcggctctat tattggaaaa aataatggaa gttagaaaag gaaaaataaa
      421 ggagattcat ttgacttttt tatctccagt aagggtgaat caagaattct cagtgcaagt
      481 agtggataac acagtgatta tttttcagca tcaaatatta tgtgtgaggg ggacatatga
      541 agaatagaga tgtttatatt ggttttgggc tacgaacacc tatcggtata aaagggaagc
      601 aatttaaaca ttaccgtcca gaacttttag gagcacacct cttaaatcaa ataaaaaaaa
      661 tagaaccaga atctaacatt gatagtatta tttgtgggaa cacagttggt actgggggca
      721 atattggtcg tttgatgact cttttttctg attatgaatc ctatattcca gtacaaacga
      781 ttgatatgca gtgtgcttca tcaagttctg ccttgttttt tggttatcta aaaatcagtg
      841 ctgatattaa tgaaaaagtt cttgttgggg ggattgaaag tagttctctt caacctatga
      901 gacgttacgc taaagaagat aatcgtaacg gagaatatac cgttgctcag ttttctcctg
      961 actcttacgc tgaaactgta atgttagaag gggcccagag agtctgtcaa aaatatggtt
     1021 ttagaagaga aatattagat aaattggcat tcttgagcca taaacgcgcc ttaacagcta
     1081 aacaaggtgg ctatttagaa gaggtaatct taccaatgga agggatgcga gatcaaggcg
     1141 ttagaaaact aaaagaaaca ttttttcaaa aattaccaag attgatggaa aattcacctt
     1201 tgctcactat tggaaatgtt tgtttaatgc acgatgctgc tgcatttcta acgcttcaga
     1261 gtcagaaaac agaattccgg attgttcaca ttgtagaagt agcaggagat cccaagctta
     1321 gtccagaatt ggttcacacg gctacggaaa aactattaac agaaactcat actaaaatat
     1381 cggattatga tgcaattgaa tggaatgaac catttgcagc gattgatgct ttatttaatc
     1441 attattatcc tgaagagaga gaaaaattca atatttttgg aggggcatta gcttacggac
     1501 acccttatgc ctgctcagga attattaata tccttcatct tatgcaggca ttaaaatata
     1561 aaaataaacc tatgggtcta actgccattg caggggcagg aggagtcgga atggccatat
     1621 caatagagta cttaggagtt aagaatgctt gaatcattga aaacaatagt taaaaccaat
     1681 agcgataaaa agttatttga tggtgattta gaggtcagtt atggagaatt ttataactta
     1741 gtcagacaag atatggcgtc acaagacaat aggaaacatg tgattagcac acactctctg
     1801 cttaaccaat tagtaagatt tgtttcaaag ttatgtcaaa aagctcttcc tattatttgt
     1861 aaacctaacc ttactcataa tgaaataagt cgattagaaa aagaagtgca gtatgctcct
     1921 caactagctg attttggtgt tctttcttct gggactacag ctgatgctaa actattgtgg
     1981 aggagcttcg cttcgtggtc tgattttttt agcattcaga atgcttattt ttcggtcact
     2041 tccaatagca gactgtttat tcaaggtgat ttttcattta ctggtaactt aaatttagcg
     2101 ctatcccttt tattactggg gggaacgctt gttgtcacac agaaaaactc tgtaaaatac
     2161 tggcagactt tatgggaaaa aactggtgta acgcatttat atctgctccc tagttatctc
     2221 aagttagtgg aacaatatag caaggagact gcgttagaca ataaaactat tattacctct
     2281 tcgcaatatg tctctgactc attactggaa ggtttatata taaagcatcc taaagtgtca
     2341 gtgaaaatct tctatggtgc tagtgagctc aattatgtgt catggtatga tggtcgtgat
     2401 atccgagata agccacagta tgttggagaa atcgttccaa atgtagctgt taggattaaa
     2461 gagggaagaa tttttgtaaa aacaccgtat agtatctgtg gcctttcaag tgaatattgt
     2521 gctggtgatt atggtgaact gatagatggc aaattatatc tatttggccg tggtggtgat
     2581 tggtgcaatc aatcgggcat taagctctat ttacctagat taatcgaaaa aataaaaaca
     2641 tgcccttata tcaaggatgc agtagcattt acaaaagaga gtcaaagtca tggacaagag
     2701 tcacattgct gtattgtttt aattgaaaat cagatggaac aagagtgttt gaagtggtta
     2761 tcggaacatt ttgaaaagaa gtatggtttt aagcattatc atattgtatc taaaattcct
     2821 ttgatgccat ctggaaaaat tgattaccaa caactcaaaa gacaattagc atgagctttt
     2881 actaaaaaaa taaaataggg ttaatggatt gcaaacctca gttctgatat tctttatttt
     2941 gaaagatatc gtgatataat gataaaaaag aaagataagg aataaaatgt tatcaaaagc
     3001 aaaatcacga tatattatcc gtgagattat taagttattt ccagatgcta aacctagctt
     3061 agactttact aatgtctttg agcttttggt agctgtaatg ctatcagctc aaacaactga
     3121 tgcagctgtt aataaagtca cccccgccct ttttgaacgc tttccaaatc ctctagtttt
     3181 ggcacaagct gatcccaaag agattgaacc ctatatttct aaaatagggc tttaccgtaa
     3241 taaagctcga ttcttaaatc aatgcgctaa acaattgata gagcattttg atggaaaagt
     3301 cccccaaact cgtcaagagc tagaaagttt gtctggcgtt ggaaggaaaa cggctaatgt
     3361 ggtcatgagt gttggatttg gtattccggc ttttgccgta gatactcatg tcactcggat
     3421 ttgtaaacac caccagattt gcaaacagtc ggcttctcca ctggaaattg agaaacgagt
     3481 catggaggtt ttaccaccag aagagtggct agctgctcat caatccatga tttattttgg
     3541 tcgtgcaatt tgtcatccta aaaatccaaa gtgcgaccag tacccacaac tttatcactt
     3601 tcctgataat ttaaagtgat tagaataaaa gagagtaact ggtgacgatt atataagcga
     3661 tacttaaaaa cggtagaaaa gctatcgttt ttttcatttt tctcataact aaacaataaa
     3721 taattcctaa ccaagcacct atttgaataa taaaaagcat ttgatgaaga ggtaaagata
     3781 agccaattgt ggctaaataa aggaaatctc cactcccgat attaatatcc accacttcag
     3841 ctaaaatccc aaataaacac aaaacaatag aaacttttgt aattggtgtg agaactatcg
     3901 ttgaaatggt gaataatatc catagaaaaa taggatactc ttggtatttt atatcgtaat
     3961 aacttaagat aaaactcatg cataaagaaa taaaatag
//
LOCUS       DBJYSH010000134         3990 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.134, whole genome shotgun sequence.
ACCESSION   DBJYSH010000134 DBJYSH010000000
VERSION     DBJYSH010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 3990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3990
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.134"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            86..1015
                     /gene="rnz"
                     /locus_tag="ACUBV3_001839"
     CDS             86..1015
                     /gene="rnz"
                     /locus_tag="ACUBV3_001839"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HHU3848316.1"
                     /translation="MEIQFLGTGAGQPAKARNVSSLVLKLLDEINEVWMFDCGEGTQR
                     QILETTIKPRKVKKIFITHMHGDHVFGLPGFLSSRAFQANEEQTDLDIYGPVGIKSFV
                     MTGLRTSGSRLPYRIHFHEFDESSLGKIMETDKFTVYAEKLDHTIFCMGYRVVQKDLE
                     GTLDAEALKLAGVPFGPLFGKVKNGENVTLEDGREIIAKDYISEPKKGKVITILGDTR
                     KTDASIRLALGADVLVHESTYGKGDERIAKSHGHSTNMQAADIAKQANAKRLLLNHVS
                     ARFMGRDCWQMEEDAKTIFSNTHLVRDLEEVGI"
     gene            1017..1778
                     /locus_tag="ACUBV3_001840"
     CDS             1017..1778
                     /locus_tag="ACUBV3_001840"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HHU3848317.1"
                     /translation="MARTILITGASGGLAQAIINQLPQDDHLIVTGRSREKLEKLYGK
                     RPNTLCLSLDITNDNAVTNMIEKIYVEFGQIDILINNAGFGSFKEFWDYSDEEVKDMF
                     AVNTFATMSIARQIGHKMSLVKSGHIVNIASMAGLIATSKASVYGASKFAVVGFLNAL
                     RLELAEKNVYVTSVNPGPIKTGFFAQADPSGDYLASIGRFVLTPEKVAKKVVSILGKN
                     KRELNLPFILAFAHKYYSLFPKTADYFARKVFNYK"
     gene            1775..3973
                     /gene="recJ"
                     /locus_tag="ACUBV3_001841"
     CDS             1775..3973
                     /gene="recJ"
                     /locus_tag="ACUBV3_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HHU3848318.1"
                     /translation="MISAKYSWVLNNQKPDAGFFEASKKEKISEAVASLIYSRGIKTS
                     AELHHFLQTNLENLHDPYLLNDMDKAVNRIRRAIENNETILVYGDYDADGMTSASIMK
                     EALDMMGAEVQVYLPNRFTDGYGPNQSVYKYFIEQQDVSLIITVDNGVAGHEAIAYAQ
                     NQGVDVVVTDHHSMPADLPCAYAIIHPEHPDANYPFPYLAGCGVAFKVACALLETIPT
                     EMLDLVAIGTIADMVSLTDENRIMVKAGLEVMKDSERIGLQELISLSNIDLKTLNEET
                     IGFKIAPQLNALGRLDDPNPAIELLTGFDDEESQAIAQMIDQKNEERKEIVQTIFDQA
                     MQMLDQTKPVQVLAKENWHPGVLGIVAGRILERTGQPVIVLNIEDGIAKGSARSVEAL
                     DIFQAFDQHRELFIAFGGHSGAAGMTLEESKVGDLSQVLCDYISKKQLDMSQKKTLTI
                     DSELRFDELSLDTVRDFEKLAPFGMDNKKPVFLLKDFKVSQARVMGQNGAHLKLKLEQ
                     DGQALDLVAFNMGSQLQEFQQAQHLELAVTLSVNQWNGATTLQLMLEDARVDGIQLFD
                     IRSKASSLPHGVPILSQEEQSKEVILLTVPDHPQELKQMTQGKQFDAIYFKNEIPKNY
                     FISGYGTRDQFASLYKTIYQFPEFDVRYKLKELSSYLHIPDILLIKMIQIFEELHFVT
                     ITEGIMTVNKEAEKRDISESQIYQELKETVKFQELMALGTPKEIYDFMMS"
BASE COUNT         1363 a          577 c          815 g         1235 t
ORIGIN      
        1 gacgtgagtc agttatttat attgatacta aatctactaa ttaaatttat taagttatgg
       61 aaggattata aaaaagtcaa caagtatgga aattcaattt ttaggaactg gagctggtca
      121 accagcgaaa gctcgtaatg tttcaagttt agtcttgaaa ttattagatg aaattaacga
      181 ggtttggatg tttgattgtg gagagggtac acagcgtcaa attttagaaa ccacgattaa
      241 acctcgtaaa gtcaaaaaaa tttttatcac gcacatgcac ggagaccatg tttttggtct
      301 tccaggtttt ttatccagtc gggcttttca agccaatgaa gaacagacag atttagatat
      361 ttatggaccg gtcggtataa aaagttttgt tatgactggc ttaaggacat caggttcacg
      421 tttaccttat cgaattcatt ttcacgagtt tgatgaatca tcactcggaa aaattatgga
      481 aacagataaa tttaccgttt atgcagaaaa gctagatcat actatctttt gtatgggata
      541 tcgtgttgtg caaaaagacc ttgagggcac tttagatgct gaagcattga aactagcagg
      601 tgttccattt ggtccccttt ttgggaaagt caaaaatggt gagaatgtta ccttagaaga
      661 tggtagagaa attattgcta aagattatat ttctgaaccg aaaaagggta aagtaatcac
      721 tattttggga gatactagaa aaacagatgc aagtattagg ttagctttgg gcgctgacgt
      781 tttagtgcac gaatctactt atggtaaggg tgatgagcga attgctaaat cgcatgggca
      841 ttcaaccaat atgcaagcag ctgatatcgc taaacaagca aatgctaaac gactattatt
      901 aaatcatgtt agtgctcgtt ttatggggcg agattgttgg caaatggaag aagatgcaaa
      961 gacgattttt tcaaataccc atcttgttag agatttggaa gaagtaggta tataaaatgg
     1021 caagaacgat actgattaca ggagcttctg gtggtttagc acaggcaatt attaaccaat
     1081 tacctcaaga cgaccattta attgtcacag ggagatcaag agaaaaactt gaaaagcttt
     1141 atggaaagag accaaacacc ttatgtttat cactagacat aactaatgac aatgctgtaa
     1201 cgaatatgat tgaaaaaatt tatgttgaat ttggtcaaat tgatatttta attaataatg
     1261 ctggatttgg ttcttttaaa gaattttggg attatagtga tgaagaagtc aaagacatgt
     1321 ttgctgtgaa tacatttgca acgatgtcta tagctcgtca gataggacat aagatgtctc
     1381 ttgtaaaatc tggtcatatc gttaatattg caagcatggc tggcctcata gcaacaagta
     1441 aagctagcgt ttatggggct agtaagtttg ctgtcgttgg atttttgaat gctcttcgtt
     1501 tagaattagc agaaaaaaat gtttatgtga caagtgtaaa ccctggacca atcaaaacag
     1561 gattttttgc tcaggctgat ccaagtggtg attatttagc aagtataggt cgttttgttc
     1621 taacaccaga aaaagtagct aaaaaagtag tttctattct tggaaaaaat aagagagaat
     1681 taaatcttcc ttttatatta gcttttgcgc ataaatatta tagtttattt ccaaaaacag
     1741 ctgattattt tgccagaaag gtattcaatt ataaatgata tctgctaagt atagttgggt
     1801 cctcaataac caaaagccag acgctggctt ttttgaggct tcaaaaaaag aaaagataag
     1861 tgaagcggtg gcttccttaa tttattcgcg aggtattaag acatcagcgg aattacatca
     1921 ttttttacaa actaatttgg aaaatctcca tgatccttat ttgttaaatg atatggataa
     1981 agctgttaat cgtattagac gagctattga aaataatgaa actattttag tttatggaga
     2041 ctatgatgct gatggaatga catcagcatc aatcatgaaa gaagcattag atatgatggg
     2101 agctgaagtc caggtttatc tcccaaatcg ctttacagat ggttacggtc ccaatcaaag
     2161 tgtttataaa tattttattg aacaacaaga tgtttcatta atcattacgg ttgataatgg
     2221 tgtggcaggg catgaagcaa ttgcatatgc tcaaaatcaa ggtgtggacg tggtagttac
     2281 tgatcatcac agtatgccag cagatttacc ttgtgcttat gctattattc acccagaaca
     2341 tcctgatgct aattatccat ttccttattt agcagggtgt ggtgttgctt tcaaagtagc
     2401 ttgtgctctc ttagaaacaa tcccgacaga aatgttggat ttggttgcta ttgggacgat
     2461 tgcagatatg gttagcctaa ctgatgaaaa tcgtatcatg gtaaaagctg gacttgaagt
     2521 tatgaaagat agtgaaagaa taggcttaca ggagctaata tctttatcaa atattgattt
     2581 aaaaacacta aatgaagaaa ctattggatt taaaattgct ccacaattaa acgctttagg
     2641 aagacttgat gaccctaatc cagctattga attactaaca gggtttgatg atgaagaatc
     2701 tcaagcaata gcccaaatga ttgatcaaaa aaatgaagag cgcaaagaaa tcgttcaaac
     2761 tatctttgac caagctatgc aaatgcttga tcaaacaaag cctgtacaag ttttagcaaa
     2821 agaaaattgg catccagggg ttttaggtat tgtggcgggt cgtattttag agagaactgg
     2881 acaacctgtt attgttttga atattgaaga tggtattgct aaaggaagtg ccagaagtgt
     2941 agaagcactt gatatttttc aagcttttga ccagcatcga gaattattca ttgcttttgg
     3001 aggtcacagc ggagcagcag gcatgacttt agaggaaagt aaggtaggtg atctttctca
     3061 agttttatgt gactatatct ctaaaaaaca attggacatg agtcagaaaa aaactctgac
     3121 aattgatagt gagctacgtt ttgacgaact tagtttagac actgttagag attttgaaaa
     3181 gttagcgcca tttggaatgg ataataaaaa accagtcttt ttgttaaaag attttaaagt
     3241 gagtcaagcc agagtcatgg ggcaaaacgg tgctcatctg aaattaaaac tagaacaaga
     3301 tggacaagca cttgatttgg tagcatttaa tatggggagt caactacaag agtttcaaca
     3361 agcacaacat ttggagttag ctgtcacttt atctgttaat caatggaatg gtgccacaac
     3421 cctacaatta atgttggagg atgcgcgtgt agatggtatt caattatttg atattagaag
     3481 caaagcaagt agcttacctc atggagtgcc aatcctgtct caagaagagc agagcaaaga
     3541 agttattctt ttgactgttc ccgatcatcc acaagaatta aaacaaatga ctcaaggtaa
     3601 acagtttgat gctatctatt ttaaaaatga gataccaaaa aattatttta tatctggtta
     3661 tggtacacgt gatcaatttg ctagtttata taaaacaata tatcaattcc cagaatttga
     3721 cgttcgttat aagttaaaag agctcagttc ctaccttcat attcctgata ttttattaat
     3781 taaaatgatt caaatttttg aagaactaca ttttgttacg attacagagg ggattatgac
     3841 agtcaataaa gaagcagaaa agcgagatat ctctgagagt caaatttatc aagaattaaa
     3901 agagacagtt aagtttcaag aattaatggc tttagggaca cctaaagaaa tatatgattt
     3961 tatgatgagt tgataatata ctaaagaagc
//
LOCUS       DBJYSH010000135         3870 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.135, whole genome shotgun sequence.
ACCESSION   DBJYSH010000135 DBJYSH010000000
VERSION     DBJYSH010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 3870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3870
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.135"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            complement(568..1206)
                     /locus_tag="ACUBV3_001842"
     CDS             complement(568..1206)
                     /locus_tag="ACUBV3_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000597402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HHU3848319.1"
                     /translation="MIKSKILLQLMQGKIVAVIRGNSQEEAFQAAQACIKGGISAIEI
                     AYTNSKASQVIEQLVTQYTNQEQVVVGAGTVLDSETARMAILAGAKFIVSPAFNLQTA
                     KLCNRYAIPYLPGCMTLSEVTTALEAGCEIIKIFPGGTLGTSFISSLKAPLPQVQIMV
                     TGGVNLTNAKDWFLSGVTAIGIGGEFNKLAALGEFDKITEMAKQYKIQLNLN"
     gene            complement(1218..2225)
                     /locus_tag="ACUBV3_001843"
     CDS             complement(1218..2225)
                     /locus_tag="ACUBV3_001843"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HHU3848320.1"
                     /translation="MSKILFFGEPLIRITPKENDYFADSISTKLFYGGSEVNTARALQ
                     GFGQDTKLLSALPNNPIGNSFLQFLKAQGIDTHSIQWVGERVGLYFLEDSFACRKGEV
                     VYDRDHSSLHDFRINQIDFDQLFEGVSLFHFSGITLSLDESIQEITLLLLKEAKKREI
                     TISLDLNFRSKLISPKNAKILFSQFATFADICFGIEPLMVDSQDTTFFNRDEATIEDV
                     KERMISLINHFDFQVIFHTKRLQDEWGRNHYQAYIANRKQEFVTSKEITTAVNQRIGS
                     GDAFVAGALYQLLQHSDSKTVIDFAVASASLKCALEGDNMFETVTAVNKVLNQSKDII
                     R"
     gene            complement(2251..2889)
                     /gene="dhuI"
                     /locus_tag="ACUBV3_001844"
     CDS             complement(2251..2889)
                     /gene="dhuI"
                     /locus_tag="ACUBV3_001844"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase DhuI"
                     /protein_id="HHU3848321.1"
                     /translation="MKIALINENSQASKNTIIYKELKAVSDEKGFEVFNYGMYGKEEE
                     SQLTYVQNGLLTAILLNSGAADFVITGCGTGIGAMLACNSFPGVVCGFAADPVDAYLF
                     SQVNGGNALSLPFAKGFGWGAELNLRYLFERLFEDEKGGGYPKERAVPEQRNARILSE
                     IKQITYRDLLSVLKEIDQDFLKETISGEHFQEYFFANCQNQNIADYLKSVLD"
     gene            complement(2906..3718)
                     /gene="dhuD"
                     /locus_tag="ACUBV3_001845"
     CDS             complement(2906..3718)
                     /gene="dhuD"
                     /locus_tag="ACUBV3_001845"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000139169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric 2-dehydro-3-deoxy-D-gluconate
                     5-dehydrogenase DhuD"
                     /protein_id="HHU3848322.1"
                     /translation="MTEQFLKDNFSLEGKVALITGASYGIGFSIATAFARAGATIVFN
                     DIKQELVDKGISAYKKLGIKAHGYVCDVTDEDGINEMVDKISQDVGVIDILVNNAGII
                     KRTPMLEMSAADFRQVIDIDLNAPFIVSKAVLPGMIQKGHGKIINICSMMSELGRETV
                     AAYAAAKGGLKMLTKNIASEYGSANIQCNGIGPGYIATPQTAPLRERQDDGSRHPFDQ
                     FIIAKTPAARWGEAEDLGAPAIFLASDASNFINGHILYVDGGILAYIGKQPQ"
BASE COUNT         1194 a          717 c          723 g         1236 t
ORIGIN      
        1 gtcgttataa aaaataaaat cagtcaaatt agaaaggagg tttagcgtgt ctaaaaaacg
       61 actattaatg atatcactca actatcaaaa cttcaaaata acggtacact caaaagctga
      121 gacgcagaaa aaatacttct gttgaaatct aataaactat aatcagaaat gtttttaatt
      181 atgtttaaaa aatgtatttg ataggagcta agttagtctg tgttgagata ggaaaagcgg
      241 gtaagcctga taggtcattg ctaagttttt ggatataaaa atgatatcaa atctattatt
      301 tgctgaaatg atatgagaat gatgagcttc actacttata tcaatctgcg ggcaagtagt
      361 atatctttgg tgaagacatg gatcagttgt cggaagtaga acggttatgt ctacaggaag
      421 attagctaaa aattatctcg gtctgataag aaaatcgaga taatgagtct gattaagata
      481 aacggtacta cagaaaaatg gctttataaa agttcttttc ttctgtctca tgattgaggt
      541 gtgggggtgc cttgtctttt tattgtgtta attcagattt agctgtattt tgtattgttt
      601 agccatttct gtgattttat caaattcccc caaagctgct agtttattga actctccacc
      661 aataccaatg gctgttactc cagatagaaa ccagtctttg gcatttgtga gatttacccc
      721 accagtcacc ataatttgta cttgaggtaa aggtgccttt agtgatgaga tgaaagatgt
      781 tcctagagta cctcctggga atattttaat tatttcgcaa ccggcttcaa gtgctgttgt
      841 tacctctgat aaggtcatgc aacctggtaa ataaggaata gcataacgat tacatagttt
      901 agcagtctgt agattaaatg cgggagatac aataaacttt gctcctgcta aaatagccat
      961 tcgggcagtt tctgagtcta gaacagttcc tgccccaacg acaacttgtt cttggttagt
     1021 atattgtgtg actagttgtt caatgacttg gctagctttg ctattagtat aggcgatttc
     1081 gatagcagaa ataccacctt ttatgcatgc ttgagcagct tgaaaagctt cttcttggct
     1141 atttccacga atgacagcaa ctatttttcc ttgcattaat tgcaacaaaa tctttgattt
     1201 tatcataatt ctcccttcta acgaataatg tctttcgatt gatttagtac tttgttaact
     1261 gcggtaacgg tctcaaacat attgtcccct tccaaagcac attttaaaga tgcactagcg
     1321 acagcgaaat caatgactgt cttgctgtct gagtgctgaa gtagttgata tagcgctccc
     1381 gccacaaaag catctccgct tccaattctc tggtttacag ctgtggtgat ttcttttgaa
     1441 gtgacaaact cttgtttacg atttgctatg taagcttggt agtggtttcg tccccattca
     1501 tcctgtagac gtttagtatg gaaaatgact tgaaagtcaa agtgatttat aagcgaaatc
     1561 attcgttctt tgacgtcctc tattgtagct tcatcgcgat tgaagaaggt agtgtcctgt
     1621 gagtcaacca taagaggttc aataccaaaa caaatatccg caaaggtagc gaactgagaa
     1681 aaaaggattt tggcattttt aggagaaatt agtttactgc gaaaattgag atctaaggag
     1741 attgttatct ctcttttttt agcttctttt aaaagaagca gtgttatctc ttggatagac
     1801 tcatccaatg atagtgtaat accactaaaa tgaaagagag aaactccttc aaataattga
     1861 tcgaaatcaa tttgatttat cctaaagtca tgtaaactac tatgatcgcg gtcgtagacc
     1921 acctcacctt ttctgcaagc aaaggaatct tctaaaaaat ataacccaac acgttctcca
     1981 acccactgaa tagaatgagt atcaataccc tgtgctttta aaaattgaag aaaactatta
     2041 cctatgggat tattgggtaa ggctgagagg agtttagtat cttgcccaaa gccttgtaga
     2101 gctcttgcgg tattaacttc agagccacca tagaatagtt tagttgagat actatctgca
     2161 aaatagtcat tttcttttgg agtgatgcga atgagaggtt caccgaaaaa aagaatctta
     2221 gacataaaag tcctttctaa aacagacgct ttagtctaag acagatttca agtagtctgc
     2281 tatattttga ttttggcaat tagcgaagaa atattcttgg aaatgctccc cagaaatagt
     2341 ttcctttaag aaatcttgat caatttcttt taaaacagag agtaaatctc tgtaagtaat
     2401 ctgttttatt tcgctaagga tacgtgcatt gcgttgctca ggtacagccc tctctttagg
     2461 gtatccccct cctttttcat cttcgaaaag acgttcaaat aaatacctca gattcaattc
     2521 agctccccaa ccaaatcctt tagcaaaagg aagggaaaga gcatttcctc cgtttacttg
     2581 agagaataag taagcgtcga caggatcagc agcgaatcca cagactacgc caggaaaact
     2641 attacaagca agcatcgctc caattcctgt cccacagcct gtaataacaa aatctgcagc
     2701 accgctatta agtaaaatag cagtcagtaa accattttgt acataagtta gctggctttc
     2761 ttcttctttg ccatacattc cgtagttgaa aacttcaaat cctttttcat cagaaaccgc
     2821 tttcaattct ttgtagataa tagtattttt acttgcttgg ctattttcgt tgattaatgc
     2881 aattttcatt tatattactc ctttattatt gaggttgttt accgatgtaa gctaaaatac
     2941 cgccatctac atagagtatg tgaccattga taaagtttga tgcatcacta gcaaggaaga
     3001 tagctggtgc acctaaatct tctgcctcac cccagcgagc ggcaggtgtt ttagcgatga
     3061 taaattggtc gaatggatga cgtgaaccat catcttgacg ttcacgaagg ggagctgttt
     3121 gtggtgtggc aatatagccg ggcccgatac cgttacattg gatgttagca gaaccgtatt
     3181 cagaagcgat gttcttggtt aacattttaa gtcccccttt ggcagcagca taggcagcaa
     3241 ctgtttcgcg tccaagctca ctcatcatgg agcaaatatt gatgatttta ccatgtcctt
     3301 tttggatcat tccaggtaat accgcttttg atacgataaa gggagcattc aaatcaatat
     3361 caataacttg tcgaaaatct gcagcgctca tctctagcat aggggtgcgt tttataatac
     3421 ctgcgttgtt aactagaata tcaattaccc caacatcttg agaaattttg tccaccattt
     3481 cgttgattcc atcttcatct gtaacatcac aaacatagcc atgggcctta atgccaagtt
     3541 ttttataggc ggagatgcct ttgtcaacga gttcttgctt aatatcattg aagacaatag
     3601 ttgctccagc tctagcaaaa gcagttgcta ttgaaaaacc aatgccgtaa gaagcgcctg
     3661 ttattaaggc gactttacct tctagtgaaa agttgtcttt taagaattgt tcagtcataa
     3721 tatgacctcc gtaaaaaaat tcaggaagta aacctgttta catgaatagt aacaaagtaa
     3781 accggttttg tcaactatag agttcttctt ctataaaact tggcaacata tataacaaag
     3841 atttatctag gttataattc ttaaagataa
//
LOCUS       DBJYSH010000136         3818 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.136, whole genome shotgun sequence.
ACCESSION   DBJYSH010000136 DBJYSH010000000
VERSION     DBJYSH010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 3818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3818
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.136"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            57..3758
                     /locus_tag="ACUBV3_001846"
     CDS             57..3758
                     /locus_tag="ACUBV3_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HHU3848323.1"
                     /translation="MDKHHSRKAILKLTLITTSILLMHSNQVNAEEQELKNQEQSPVI
                     ANVAQQPSPSVTTNIVEKTSVTAASASNTAKEMGDTSVKNDKTEDELLEELSKNLDTS
                     NWGADLEEEYPSKPETTNNKENNVVTNASTAIAQKVPSAYEEVKPESKSSLAVLDTSK
                     ITKLQATTQRGKGNVVAIIDTGFDINHDIFRLDSPKDDKHSFKTKAEFEELKAKHNIT
                     YGKWVNDKIVFAHNYANNTETVADIAAAMKDGYGSEAKNISHGTHVAGIFVGNSKRPA
                     INGLLLEGAAPNAQVLLMRIPDKIDSDKFGEAYAKAITDAVNLGAKTINMSLGKTADS
                     LIALNDKVKLALKLASEKGVAVVVAAGNEGAFGMDYSKPLSTNPDYGTVNSPAISEDT
                     LSVASYESHKTISEVVETTIEGKLVKLPIVTSKPFDKGKAYDVVYANYGAKKDFEGKD
                     FKGKIALIERGGGLDFMTKITHATNAGVIGIVIFNDQEKRGNFLIPYRELPVGVISKV
                     DGERIKNTSSQLTFNQSFEVVDSQGGNRILEQSSWGVTAEGAIKPDVTASGFEIYSLT
                     YNNQYQTMSGTSMASPHVAGLMTMLQSHLAEKYKGMNLDSKKLLELSKNILMSSATAL
                     YSEEDKAFYSPRQQGAGVVDAEKAIQAQYYVTGNDGKAKINLKRVGDKFDITVTIHKL
                     VEGVKELYYQANVATEQVNKGKFALKPQALLDTNWQKVILRDKETQVRFTIDSSQFSQ
                     KLKEQMANGYFLEGFVRFKEAKDSNQELMSIPFVGFNGDFANLQALETPIYKTLSKGN
                     FYYKPNDTTHKDQLEYNESAPFESNNYTALLTQSASWGYVDYVKNGGELELAPESPKR
                     IILGTFENKVEDKTIHLLERDAANNPYFAISPNKDGNRDEITPQATFLRNVKDISAQV
                     LDQNGNVIWQSKVLPSYRKNFHNNPKQSDGHYRMDALQWSGLDKDGKVVADGFYTYRL
                     RYTPVAEGANSQESDFKVQVSTKSPNLPSRAQFDETNRTLSLAMPKESSYVPTYRLQL
                     VLSHVVKDEEYGDETSYHYFHIDQEGKVTLPKTVKIGESEVAVDPKTLTLVVEDKAGN
                     FATVKLSDLLNKAVVSEKENAIVISNNFKYFDNLKKEPMFISKEGKVVNKNLEEIALV
                     KPQTTVTTQSLSKEITQSGNEKVLTSTNNNSSRVAKIISPKHNGDSVNHTLPSTSDRA
                     TNGLFVGTLALLSSLLLYLKPKKTKNNSK"
BASE COUNT         1385 a          589 c          745 g         1099 t
ORIGIN      
        1 acattaatta tgttgtaata tagtatgcat actgattttt ggaggaaata ggaaaagtgg
       61 ataaacatca ctcaagaaag gctattttaa agttaacact tataacaact agtattttat
      121 taatgcatag caatcaagtg aatgcagagg agcaagaatt aaaaaaccaa gagcaatcac
      181 ctgtaattgc taatgttgct caacagccat cgccatcagt aactactaat attgttgaaa
      241 aaacatctgt aacagctgct tctgctagta atacagcgaa agaaatgggt gatacatctg
      301 taaaaaatga taaaacagaa gatgaattat tagaagagtt atctaaaaac cttgatacgt
      361 ctaattgggg ggctgatctt gaagaagaat atccctctaa accagagaca accaacaata
      421 aagaaaacaa tgtagtaaca aatgcttcaa ctgcaatagc acagaaagtt ccctcagcgt
      481 atgaagaggt gaagccagaa agcaagtcat cgcttgctgt tcttgataca tctaaaataa
      541 caaaattaca agccacaacc caaagaggaa agggaaatgt agtagctatt attgatactg
      601 gctttgatat taaccatgat atttttcgtt tagatagccc aaaagatgat aagcacagct
      661 ttaaaactaa ggcagaattt gaggaattaa aagcaaaaca taatatcact tatgggaaat
      721 gggttaacga taagattgtt tttgcacata actacgccaa caatacagaa acggtggctg
      781 atattgcagc agctatgaaa gatggttatg gttcagaagc aaagaatatt tcgcatggta
      841 cacacgttgc tggtattttt gtaggtaata gtaaacgtcc agcaatcaat ggtcttcttt
      901 tagaaggtgc agcgccaaat gctcaagtct tattaatgcg tattccagat aaaattgatt
      961 cggacaaatt tggagaagca tatgctaaag caatcacaga cgctgttaat ctaggagcaa
     1021 aaacgattaa tatgagcctt ggaaaaacag cagattcttt aattgctctc aatgataaag
     1081 ttaaattagc acttaaatta gcttctgaga agggcgttgc agttgttgtg gctgccggaa
     1141 atgaaggtgc atttggtatg gattatagca aaccattatc aactaatcct gactacggta
     1201 cggttaatag tccagctatt tctgaagata ctttgagtgt tgctagctat gaatcacata
     1261 aaactatcag tgaggtcgtt gaaacaacta ttgaaggtaa gttagttaag ttgccgattg
     1321 tgacttctaa accttttgac aaaggtaagg cctacgatgt ggtttatgcc aattatggtg
     1381 caaaaaaaga ctttgaaggt aaggacttta aaggtaagat tgcattaatt gagcgtggtg
     1441 gtggacttga ttttatgact aaaatcactc atgctacaaa tgcaggtgtt attggtatcg
     1501 ttatttttaa cgatcaagaa aaacgtggaa attttctaat tccttaccgt gaattacctg
     1561 tgggggttat tagtaaagta gatggcgagc gtataaaaaa tacttcaagt cagttaacat
     1621 ttaaccagag ttttgaagta gttgatagcc aaggtggcaa tcgtatactg gaacaatcaa
     1681 gttggggcgt gacagctgaa ggagcaatca agcctgatgt aacagcttct ggctttgaaa
     1741 tttattcttt aacctataat aatcaatacc aaacaatgtc tggtacaagt atggcttcac
     1801 cacatgttgc aggattaatg acaatgcttc aaagtcattt ggctgagaaa tataaaggga
     1861 tgaatttaga ttctaaaaaa ttgctagaat tgtctaaaaa catcctcatg agctcagcaa
     1921 cagcattata tagtgaagag gataaggcgt tttattcacc acgtcagcaa ggtgcaggtg
     1981 tagttgatgc tgaaaaagct atccaagctc aatattatgt tactggaaac gatggcaaag
     2041 ctaaaattaa tctcaaacga gtgggagata aatttgatat cacagttaca attcataaac
     2101 ttgtagaagg tgtcaaagaa ttgtattatc aagctaatgt agcaacagaa caagtaaata
     2161 aaggtaaatt tgcccttaaa ccacaagcct tgctagatac taattggcag aaagtaattc
     2221 ttcgtgataa agaaacacaa gttcgattta ctattgattc tagtcaattt agtcagaaat
     2281 taaaagaaca gatggcaaat ggttatttct tagaaggttt tgtacgtttt aaagaagcca
     2341 aggatagtaa tcaggagtta atgagtattc cttttgtagg atttaatggt gattttgcga
     2401 acttacaagc acttgaaaca ccgatttata agacgctttc taaaggtaat ttctactata
     2461 aaccaaatga tacaactcat aaagaccaat tggagtacaa tgaatcagct ccttttgaaa
     2521 gcaacaacta tactgccttg ttaacacaat cagcgtcttg gggctatgtt gattatgtca
     2581 aaaatggtgg ggagttagaa ttagcaccgg agagtccaaa aagaattatt ttaggaactt
     2641 ttgagaataa ggttgaggat aaaacaattc atcttttgga aagagatgca gcgaataatc
     2701 catattttgc catttctcca aataaagatg gaaataggga tgaaatcact ccccaggcaa
     2761 ctttcttaag aaatgttaag gatatttctg ctcaagttct agatcaaaat ggaaatgtta
     2821 tttggcaaag taaggtttta ccatcttatc gtaaaaattt ccataataat ccaaagcaaa
     2881 gtgatggtca ttatcgtatg gatgcccttc agtggagtgg tttagataag gatggcaaag
     2941 ttgtagcaga tggtttttat acttatcgtt tacgttacac accagtagca gaaggagcaa
     3001 atagtcagga gtcagacttt aaagttcaag taagtactaa gtcaccaaat cttccttcac
     3061 gagctcagtt tgatgaaact aatcgaacat taagcttagc catgcctaag gaaagtagtt
     3121 atgttcctac atatcgtcta caattagttt tatctcatgt tgtaaaagat gaagaatatg
     3181 gagatgagac ttcttaccat tatttccata tagatcaaga aggtaaagtg acacttccta
     3241 aaacagttaa gataggagag agtgaggttg cagtagaccc taagaccttg acacttgttg
     3301 tggaagataa agctggtaat ttcgcaacgg taaaattgtc tgacctcttg aataaggcag
     3361 tagtatcaga gaaagaaaac gctatagtaa tttctaacaa tttcaaatat tttgataact
     3421 tgaaaaaaga acctatgttt atttctaaag aaggaaaagt agtaaacaag aatctagaag
     3481 aaatagcatt agttaagccg caaactacag ttactactca atcattgtct aaagaaataa
     3541 ctcaatcagg aaatgagaaa gtcctcactt ctacaaacaa taatagtagc agagtagcta
     3601 agatcatatc acctaaacat aacggggatt ctgttaacca taccttacct agtacatcag
     3661 atagagcaac gaatggtcta tttgttggta ctttggcatt gttatctagt ttacttcttt
     3721 acttgaaacc caaaaagact aaaaataata gtaaataaaa ctaagtaaac tactctgcaa
     3781 tatgatgaaa agttcagttt aacattatgt taagctgg
//
LOCUS       DBJYSH010000137         3756 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.137, whole genome shotgun sequence.
ACCESSION   DBJYSH010000137 DBJYSH010000000
VERSION     DBJYSH010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 3756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3756
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.137"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            188..580
                     /gene="merR"
                     /locus_tag="ACUBV3_001847"
     CDS             188..580
                     /gene="merR"
                     /locus_tag="ACUBV3_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020220203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0045340 - mercury ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0046689 - response to mercury ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hg(II)-responsive transcriptional regulator"
                     /protein_id="HHU3848324.1"
                     /translation="MIYRISEFADKCGVNKETIRYYERKNLLQEPHRTEAGYRIYSYD
                     DVKRVGFIKRIQELGFSLSEIYKLLGVVDKDEVRCQDMFEFVSKKQKEVQKQIEDLKR
                     IETMLDDLKQRCPDEKKLHSCPIIETLT"
     gene            609..2249
                     /gene="merA"
                     /locus_tag="ACUBV3_001848"
     CDS             609..2249
                     /gene="merA"
                     /locus_tag="ACUBV3_001848"
                     /EC_number="1.16.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001031939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016152 - mercury (II) reductase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0045340 - mercury ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0046689 - response to mercury ion [Evidence
                     IEA];
                     GO_process: GO:0050787 - detoxification of mercury ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mercury(II) reductase"
                     /protein_id="HHU3848325.1"
                     /translation="MNKFKVNISGMTCTGCEKHVESALEKIGAKNIESSYRRGEAVFE
                     LPDDIEVESAIKAIDEANYQAGEIEEVSSLENVALINEDNYDLLIIGSGAAAFSSAIK
                     AIEYGAKVGMIERGTVGGTCVNIGCVPSKTLLRAGEINHLSKDNPFIGLQTSAGEVDL
                     ASLITQKDKLVSELRNQKYMDLIDEYNFDLIKGEAKFVDASTVEVNGTKLSAKRFLIA
                     TGASPSLPQISGLEKMDYLTSTTLLELKKIPKRLTVIGSGYIGMELGQLFHHLGSEIT
                     LMQRSERLLKEYDPEISESVEKALIEQGINLVKGATFERVEQSGEIKRVYVTVNGSRE
                     VIESDQLLVATGRKPNTDSLNLSAAGVETGKNNEILINDFGQTSNEKIYAAGDVTLGP
                     QFVYVAAYEGGIITDNAIGGLNKKIDLSVVPAVTFTNPTVATVGLTEEQAKEKGYDVK
                     TSVLPLGAVPRAIVNRETTGVFKLVADAETLKVLGVHIVSENAGDVIYAASLAVKFGL
                     TIEDLTETLAPYLTMAEGLKLVALTFDKDISKLSCCAG"
     gene            2409..3716
                     /locus_tag="ACUBV3_001849"
     CDS             2409..3716
                     /locus_tag="ACUBV3_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HHU3848326.1"
                     /translation="MSDLLSLPDIKTIEPPQENETDMMFKVEAVGPPERCPECGFDKL
                     YKHSSRNQLIMDLPIRLKRVGLHLNRRRYKCRECGSTISVDEKRSMTKRLLKSIQEQS
                     MSKTFVEVAESVGVDEKTIRNVFKDYVALKEREYQFETPKWLGIDEIHIIRRPRLVLT
                     NIERRTIYDIKPNRNKETVIQRLSEISDRTYIEYVTMDMWKPYKDAVNTILPQAKVVV
                     DKFHVVRMANQALDNVRKSLKAHMSQKERRTLMRERFILLKRKHDLNERESFLLDTWL
                     GNLPALKEAYELKEEFYWIWDTPDPDEGHLRYSQWRHRCMSSNSKDAYKDLVRAVDNW
                     HVEIFNYFDKRLTNAYTESINSIIRQVERMGRGYSFDALRAKILFNEKLHKKRKPRFN
                     SSAFNKAMLYDTFNWYEVNDHDITDNLGVDFSTLIKNLEKGDL"
BASE COUNT         1314 a          580 c          788 g         1074 t
ORIGIN      
        1 ttcttaatag tttaaaaaca actggtgttt aaatcggatt ttctggtggg atgttaggat
       61 aatcaatagt attatataaa agtatcaatc acatacgagt gatactttgg gaaaaaaata
      121 aaatagtcca atttacttga ccgtgtacta tggtacatgg tttataatat tcagggggtg
      181 aaataagatg atttatcgca ttagtgagtt tgcagataaa tgtggagtta ataaagaaac
      241 gatcagatat tacgagcgaa aaaatttatt acaagaacct caccgaacgg aagctggtta
      301 tcggatatat tcatatgatg acgttaagcg tgttgggttt attaaacgaa tacaggaact
      361 tggtttctct ttaagcgaga tttataaatt acttggtgtt gtagataaag atgaagttcg
      421 ttgtcaagat atgttcgaat ttgtttctaa aaaacaaaag gaagtgcaaa aacaaataga
      481 ggatttaaaa cgaattgaaa ctatgttaga cgacttaaaa caacgatgtc cagatgaaaa
      541 gaaattacat tcgtgtccaa taatagaaac attaacatga gagattaatt aacgaaagga
      601 gcttttttat gaataaattt aaggtaaaca tttcaggaat gacttgtacg ggttgtgaaa
      661 aacacgtaga atcagcactt gaaaagatag gtgctaaaaa tattgagtct agttatcgtc
      721 gtggtgaagc agtatttgaa ctgcccgatg atattgaggt tgaaagtgca ataaaggcga
      781 ttgatgaagc aaattaccaa gccggagaaa ttgaagaagt atcatcacta gaaaacgtgg
      841 cgttaattaa tgaagacaat tatgaccttc ttattattgg ttctggtgct gctgcctttt
      901 cttcggcaat taaagctata gaatacggtg caaaagttgg aatgattgag cgtggaacgg
      961 ttgggggaac ctgtgtgaat attggctgtg ttccgtcaaa aactcttctt agggcagggg
     1021 aaatcaatca tttatcaaaa gacaatccgt ttataggttt acaaacatcc gctggagaag
     1081 tggatttagc tagtttaatc acgcaaaagg ataaattggt gagcgaactt cggaatcaaa
     1141 aatatatgga tttaattgat gaatataatt ttgatttaat taaaggtgaa gcaaaattcg
     1201 ttgatgctag tacggttgag gtcaatggga caaagttatc tgcaaaacgc tttttaattg
     1261 caacaggtgc atcgccttcg ttgccccaaa tttcaggact tgaaaaaatg gactatttaa
     1321 ctagtacaac acttcttgag ttaaagaaaa taccaaaacg attaactgta attggttcag
     1381 gatacattgg aatggagctt ggacaactat ttcatcattt aggttcagaa ataacgctta
     1441 tgcaaagaag tgagcgactt ttaaaggagt atgatcctga gatttcagag tcagttgaaa
     1501 aagcgttaat tgaacagggt ataaaccttg tcaaaggggc aacttttgag cgtgttgaac
     1561 aaagtggaga gataaaaagg gtttacgtaa cagtaaatgg cagtagagaa gtcattgaat
     1621 cagatcagtt acttgttgcc actggaagaa aaccaaatac ggattcttta aatttaagtg
     1681 cagcaggtgt tgaaactgga aaaaataatg aaatcctgat caatgatttt ggtcaaacaa
     1741 gtaatgaaaa gatttatgca gcaggagatg tgactttagg accacaattt gtatatgtag
     1801 cagcctatga aggtggaatt attactgata atgctattgg tggattaaac aaaaaaatag
     1861 atttatcggt agttcctgct gttacgttta cgaatccgac ggttgcaacg gttggtttaa
     1921 cagaagaaca agcaaaagag aaagggtatg atgtgaagac atctgtatta cctttaggtg
     1981 ctgttccaag agcaattgta aaccgtgaaa caaccggtgt atttaaacta gtagcagatg
     2041 cagaaacact aaaagtatta ggggttcata ttgtatctga aaatgcagga gatgtcatct
     2101 atgcagcatc attagctgtt aaatttggct taacgataga agatttaaca gaaaccctag
     2161 caccatattt aacaatggct gaagggctaa aattagttgc acttacgttc gataaagata
     2221 tttcgaaatt atcttgttgt gcaggctaag ggaacttttt tacaactccc tattcaagtg
     2281 aaccagctaa tgctttaagg gattttctaa gtgcatttgt ggtcacaaaa ataatttaac
     2341 actgatgatt tcgacatcaa atctataatt gataccttaa caccgcagag taataaagga
     2401 tgaataatat gtcagactta ttatccctac cagacattaa aacaatagaa ccgccacaag
     2461 aaaatgaaac cgatatgatg tttaaagttg aagcagtcgg accacctgaa cgttgtcctg
     2521 aatgtggttt tgacaagttg tacaaacaca gttcaagaaa tcaactaatt atggatttgc
     2581 ccattcgttt aaagcgagtg ggcttacatt tgaaccgtag acgatacaag tgtcgtgaat
     2641 gcggatctac catatctgta gatgaaaagc gtagtatgac caaaaggctt ttaaagtcca
     2701 ttcaagagca atccatgtct aagacctttg tagaagtcgc agaaagcgtt ggtgttgacg
     2761 agaaaaccat taggaacgtt tttaaggact atgtggcact caaagaacgt gaataccagt
     2821 ttgaaactcc taagtggctt gggatagacg agatacatat tatccgtaga cctcggcttg
     2881 tattgactaa tattgaacgc aggactattt atgacatcaa gcctaaccgt aacaaggaaa
     2941 cagtcatcca acgtctttca gaaatcagtg acaggactta cattgagtac gtcacaatgg
     3001 atatgtggaa gccctacaaa gacgcagtga acactatcct tccacaagct aaagttgtcg
     3061 tagataagtt tcatgtagtt agaatggcta atcaagcctt agataacgtc agaaagtctt
     3121 tgaaagccca tatgagccaa aaagaaagac gtacccttat gcgtgaaagg tttatccttc
     3181 taaagcgtaa acacgatcta aatgaacgtg aatcattcct cttagatact tggttaggta
     3241 atcttcctgc tttaaaagaa gcctatgaac tcaaagaaga gttttactgg atatgggata
     3301 ctcctgatcc agatgaaggt catcttcgtt atagtcaatg gagacaccgt tgtatgtcca
     3361 gcaactctaa agacgcatat aaagacctcg tgagagccgt agacaactgg catgttgaaa
     3421 tattcaacta ctttgataaa aggctcacta atgcttatac ggagtcaatt aacagcatta
     3481 ttaggcaggt agagcgaatg ggtagaggtt actcgtttga tgccttacga gccaaaatcc
     3541 ttttcaatga gaagctccat aaaaagcgta agccacgatt taattcaagt gctttcaata
     3601 aagctatgtt atacgatact ttcaattggt atgaagtgaa tgatcacgac attacagaca
     3661 acttaggtgt cgatttttcc acacttatta agaatttgga gaagggtgat ttataagccc
     3721 ttttccacca taaaatccga atacccaatt taaaaa
//
LOCUS       DBJYSH010000138         3715 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.138, whole genome shotgun sequence.
ACCESSION   DBJYSH010000138 DBJYSH010000000
VERSION     DBJYSH010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 3715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3715
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.138"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            complement(<1..1424)
                     /locus_tag="ACUBV3_001850"
     CDS             complement(<1..1424)
                     /locus_tag="ACUBV3_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HHU3848327.1"
                     /translation="MTKEYQNYVNGEWKSSVNQIEILSPIDDSSLGFVPAMTREEVDH
                     AMKAGREALPAWAALTVYERAQYLHKAADIIERDKEEIATVLAKEISKAYNASVTEVV
                     RTADLIRYAAEEGIRLSTSADEGGKMDASTGHKLAVIRRQPVGIVLAIAPYNYPVNLS
                     GSKIAPALIGGNVVMFKPPTQGSVSGLVLAKAFAEAGLPAGVFNTITGRGSEIGDYIV
                     EHEEVNFINFTGSTPVGQRIGKLAGMRPIMLELGGKDAGIVLADADLDNAAKQIVAGA
                     YDYSGQRCTAIKRVLVVEEVADELAEKISENVAKLSVGDPFDNATVTPVIDDNSADFI
                     ESLVVDARQKGAKELNEFKRDGRLLTPGLFDHVTLDMKLAWEEPFGPILPIIRVKDAE
                     EAVAIANKSDFGLQSSVFTRDFQKAFDIANKLEVGTVHINNKTGRGPDNFPFLGLKGS
                     GAGVQGIRYSIEAMTNVKSIVFDM"
     gene            complement(1574..3307)
                     /gene="ptsP"
                     /locus_tag="ACUBV3_001851"
     CDS             complement(1574..3307)
                     /gene="ptsP"
                     /locus_tag="ACUBV3_001851"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HHU3848328.1"
                     /translation="MTEMLKGIAASDGVAVAKAYLLVQPDLSFETVTVEDTNAEEARL
                     DVALQASQDELSVIREKAVESLGEEAAAVFDAHLMVLSDPEMINQIKETIRAKQVNAE
                     TGLKEVTDMFITIFEGMEDNPYMQERAADIRDVAKRVLAHLLGVKLPNPATINEESIV
                     IAHDLTPSDTAQLNKQFVKAFVTNIGGRTSHSAIMARTLEIAAVLGTNDITERVQDGQ
                     LIAVNGITGEVIIEPTEAQISAFKAAGEAYAKQKAEWALLKDAQTVTADGKHFELAAN
                     IGTPKDVEGVNENGAEAVGLYRTEFLYMDSQDFPTEDEQYEAYKAVLEGMNGKPVVVR
                     TMDIGGDKELPYFDLPKEMNPFLGFRALRISISETGDAMFRTQIRALLRASVHGQLRI
                     MFPMVALLKEFRAAKAIFEEEKANLLADGVAVAEGIEVGIMIEIPAAAMLADQFAKEV
                     DFFSIGTNDLIQYTMAADRMNEQVSYLYQPYNPSILRLINNVIKAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSVLRTRSLMKKLDTAKMEEYANRALSECSTMEEV
                     IELQKEYVDFD"
     gene            complement(3312..3575)
                     /locus_tag="ACUBV3_001852"
     CDS             complement(3312..3575)
                     /locus_tag="ACUBV3_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019775469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HHU3848329.1"
                     /translation="MASKDFHIVAETGIHARPATLLVQTASKFASDITLDYKGKAVNL
                     KSIMGVMSLGVGQGADVTISAEGADADDAIAAIEETMTKEGLA"
BASE COUNT         1088 a          799 c          633 g         1195 t
ORIGIN      
        1 ttcatatcaa aaacaatcga ttttacattt gtcattgctt caattgaata tctgatacct
       61 tgaacacctg caccagatcc tttgagtcct aagaatggga agttatcagg accacgtcca
      121 gtcttattgt taatgtgaac tgtaccaact tcaagtttat ttgctatatc aaatgctttt
      181 tggaaatcac gtgtaaagac tgatgattgt aatccaaaat cagatttgtt ggcaatagca
      241 acagcttctt ctgcatcctt gacacgaata attgggagaa ttggtccaaa aggctcttcc
      301 caagctagtt tcatatctaa agtaacatga tcaaacaatc ctggagttaa tagacgacca
      361 tcacgtttaa attcattcaa ttctttcgca cctttttgac gtgcatctac tactaagctt
      421 tcaataaagt cagctgaatt atcatcaata accggtgtca ccgttgcatt atcaaatgga
      481 tcacctactg ataattttgc tacattttca gatatttttt ctgccaattc atctgcaact
      541 tcttcaacga caagcacacg cttaattgcc gtacagcgtt gtccagagta atcataagca
      601 cctgcaacga tttgtttagc agcgttatca aggtcagcat cagctaagac gatacctgca
      661 tccttaccgc ccaactcaag cataattgga cgcattcctg ccaacttacc aatacgttgt
      721 ccaactggcg ttgatcctgt aaagttaata aaattaactt cttcatgctc aacaatgtaa
      781 tctccaatct cagaaccgcg tcctgtaata gtattaaaga cacctgctgg aagacctgct
      841 tctgcaaaag cttttgctaa aacaagtcct gagactgaac cttgtgttgg tggtttaaac
      901 atcacaacgt ttccaccaat tagagctggc gcaatttttg atcctgagag gttaacaggg
      961 taattataag gtgcgattgc taaaacgata cctactggtt gacgacgaat aacagccaac
     1021 ttatgacctg ttgaagcatc catttttcca ccttcgtcag ctgaagttga taaacgaatt
     1081 ccttcttctg ctgcataacg aataagatca gctgtcctta caacctcagt tactgaagca
     1141 ttgtaagctt tagaaatttc ttttgctaaa acagtagcaa tttcttcttt atcacgttca
     1201 ataatgtctg cggctttatg aaggtattgt gcacgttcat atactgttaa agcagcccaa
     1261 gctggtaaag cctcacgacc cgctttcata gcatgatcaa cttcttctcg agtcatcgct
     1321 ggcacgaatc ccaatgaaga atcatcaatt ggtgacaaaa tctcaatctg attaacagat
     1381 gatttccatt cgccattgac ataattttga tattcttttg tcaaagtaaa tctcctttta
     1441 cattgtaata ggtttattct atcactttat ctatttattt caattatctg acatattttt
     1501 cttagaaact taacaatatc caaactttca ataaaaaagt agagattttt taatccctac
     1561 ttttttatta ttctcagtca aagtcaacgt attctttttg aagttcgata acttcttcca
     1621 tagttgaaca ctctgaaaga gcacgattag cgtattcttc cattttagct gtgtccaatt
     1681 ttttcattaa gctacgtgta cgaagtactg aggttgctga cattgagaat tcatccaatc
     1741 ccataccaac aagaagtgga accgcagttt gatcaccggc catctcacca cacatacctg
     1801 cccatttacc ttcagcatga gcagctttga taacgttgtt aatcaaacga aggattgatg
     1861 gattgtatgg ttgatagagg tatgaaactt gctcattcat acggtccgca gccattgtgt
     1921 attggataag atcattagta ccaattgaga agaaatcaac ttcttttgca aattggtctg
     1981 caagcatagc tgccgctggg atttcaatca taataccaac ttcaatgcct tcagctactg
     2041 ctacaccgtc agctaacaag ttcgctttct cttcttcaaa tatagcttta gcagcacgga
     2101 attctttaag caaagcaacc attgggaaca tgatgcgtag ctgtccatga actgaagcac
     2161 gaagaagtgc tcggatttgt gtgcggaaca ttgcatcacc agtttcagaa attgaaatac
     2221 gcaatgcacg gaatcctaag aatgggttca tctcttttgg aagatcaaag taaggaagct
     2281 ctttatctcc accgatatcc attgtacgaa caacaactgg tttaccattc ataccctcaa
     2341 gaactgcttt atatgcttca tattgctcat cttctgttgg gaaatcttgt gaatccatat
     2401 ataagaactc tgtacggtaa agaccaactg cttctgctcc attttcgtta acaccctcaa
     2461 catctttagg tgtaccgata ttagcagcta attcaaaatg tttaccatca gcagtaactg
     2521 tttgtgcatc cttaagaagt gcccattcag ctttttgctt ggcataagct tcacctgctg
     2581 ccttgaaggc tgaaatttga gcttcagttg gttcaatgat aacttcacca gtaataccat
     2641 taacagcaat caactgacca tcttgaacac gttcagtaat atcatttgta ccaagaacag
     2701 ctgcgatttc gagtgtacgt gccataatag ctgagtgact tgtacgccct ccaatatttg
     2761 ttacaaaagc tttaacaaat tgtttattta attgagctgt atcagaaggt gtcaaatcgt
     2821 gtgcaataac gattgattct tcattgatag ttgccgggtt tggtaattta acacccaaaa
     2881 gatgtgctaa gacacgtttg gcaacatcac ggatatctgc tgcacgttct tgcatgtatg
     2941 ggttatcttc cataccttca aagatagtga tgaacatatc agtcacttct ttaagacctg
     3001 tttctgcatt tacttgtttt gcacgaatag tctctttaat ttgattaatc atttctgggt
     3061 cagaaagaac cattaaatga gcatcaaata cagctgcggc ttcttcgcct aagctttcta
     3121 ctgccttctc acggataaca gaaagctcgt cttgtgaagc ttggagtgca acatcaaggc
     3181 gagcttcttc tgcgtttgta tcttcgactg ttacagtctc aaatgacaaa tccggttgga
     3241 caagtagata tgctttagca actgcaacac catcagaggc tgcgattcct ttaagcattt
     3301 ctgtcatttt cttatgccaa tccttctttt gtcattgttt cttcgatagc tgcaattgca
     3361 tcatctgcat ctgcaccttc tgctgaaata gtaacatcag caccttgacc aacaccaaga
     3421 ctcataacac ccatgattga tttaaggttt actgctttac ctttgtaatc aagcgtgatg
     3481 tctgaagcaa atttgctagc tgtttggaca agcaaagtag ctggacgtgc gtgaatacct
     3541 gtttctgcaa caatgtggaa atcttttgaa gccatattat ggttctcctt ttgtcatttg
     3601 taaatatttg agtcacctat tggtaaccct tacaagttaa aagtataaca tattgctttt
     3661 tatttttcaa gattaaatgt ctcaaacaga aaaaaacttg aatgttttcc acagc
//
LOCUS       DBJYSH010000139         3659 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.139, whole genome shotgun sequence.
ACCESSION   DBJYSH010000139 DBJYSH010000000
VERSION     DBJYSH010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 3659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3659
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.139"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            <1..306
                     /locus_tag="ACUBV3_001853"
     rRNA            <1..306
                     /locus_tag="ACUBV3_001853"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            361..433
                     /locus_tag="ACUBV3_001854"
     tRNA            361..433
                     /locus_tag="ACUBV3_001854"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:394..396,aa:Ala,seq:tgc)
     gene            588..3490
                     /locus_tag="ACUBV3_001855"
     rRNA            588..3490
                     /locus_tag="ACUBV3_001855"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            3565..>3659
                     /gene="rrf"
                     /locus_tag="ACUBV3_001856"
     rRNA            3565..>3659
                     /gene="rrf"
                     /locus_tag="ACUBV3_001856"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         1045 a          738 c         1043 g          833 t
ORIGIN      
        1 aatggttggt acaacgagtc gcaagccggt gacggcaagc taatctctta aagccaatct
       61 cagttcggat tgtaggctgc aactcgccta catgaagtcg gaatcgctag taatcgcgga
      121 tcagcacgcc gcggtgaata cgttcccggg ccttgtacac accgcccgtc acaccacgag
      181 agtttgtaac acccgaagtc ggtgaggtaa ccttttagga gccagccgcc taaggtggga
      241 tagatgattg gggtgaagtc gtaacaaggt agccgtatcg gaaggtgcgg ctggatcacc
      301 tcctttctaa ggataaggaa acctgccatt tgcgtcttgt ttagttttga gaggtcttgt
      361 ggggccttag ctcagctggg agagcgcctg ctttgcacgc aggaggtcag cggttcgatc
      421 ccgctaggct ccattgaatc gaaaggttca aattgttcat tgaaaattga atatctatat
      481 caaattccac gatctagaaa tagattgtag aaagtaacaa gaaaataaac cgaaaacgct
      541 gtgaatattt aatgagtttt ctagttttaa agaaactagg ttaataaggt taagttaata
      601 agggcgcacg gtggatgcct tggcactaga agccgaagaa ggacgtgact aacgacgaaa
      661 tgctttgggg agctgtaagt aagcgctgat ccagagatgt ccgaatgggg gaacccacta
      721 gttaatgact agtatccata tctgttaagg atatgagaag gaagacgcag tgaactgaaa
      781 catctaagta gctgcaggaa gagaaagcaa aagcgattgc cttagtagcg gcgagcgaaa
      841 cggcaggagg gcaaaccgaa gtgtttacac ttcggggttg taggactgcg atataggatt
      901 aatcattata gaagaattac ctgggaaggt aagccaaaga gagtaacagc ctcgtattta
      961 aaatgataga ttaaccttag cagtatcctg agtacggcga gacacgcgaa atctcgtcgg
     1021 aatctgggag gaccatctcc caaccctaaa tactctctag tgaccgatag tgaaccagta
     1081 ccgtgaggga aaggtgaaaa gcaccccggg aggggagtga aatagaacct gaaaccgtgt
     1141 gcctacaaca agttcgagcc cgttaatggg tgagagcgtg ccttttgtag aatgaaccgg
     1201 cgagttacga tatgatgcga ggttaagttg aagagacgga gccgtaggga aaccgagtct
     1261 taatagggcg ccatagtatc atgttgtaga cccgaaacca tgtgacctac ccatgagcag
     1321 gttgaaggtg aggtaaaact cactggagga ccgaaccagg gcacgttgaa aagtgcttgg
     1381 atgacttgtg ggtagcggag aaattccaaa cgaacttgga gatagctggt tctctccgaa
     1441 atagctttag ggctagcgtc gatgttaagt ctcttggagg tagagcactg tttgggtgag
     1501 gggtccatcc cggattacca atctcagata aactccgaat gccaacgaga tataatcggc
     1561 agtcagactg cgagtgctaa gatccgtagt cgaaagggaa acagcccaga ccaccagcta
     1621 aggtcccaaa atatatgtta agtggaaaag gatgtggggt tgcacagaca actaggatgt
     1681 tagcttagaa gcagctattc attcaaagag tgcgtaatag ctcactagtc gagtgaccct
     1741 gcgccgaaaa tgtaccgggg ctaaaacata ttaccgaagc tgtggatgac acaaaagtgt
     1801 catggtagga gagcgttcta tgtgtgaaga aggtgtaccg tgaggagcgc tggaacgcat
     1861 agaagtgaga atgccggtat gagtagcgca agacaggtga gaatcctgtc caccgtaaga
     1921 ctaaggtttc caggggaagg ctcgtccgcc ctgggttagt cgggacctaa ggagagaccg
     1981 aaaggtgtat ccgatggaca acaggttgat attcctgtac tagagtatat agtgatggag
     2041 ggacgcagta ggctaactaa accagacgat tggaagtgtc tggtcaaaca gtgaggtgtg
     2101 atatgagtca aatgcttata tctttaacat tgagctgtga cgaggagcga agtttagtag
     2161 cgaagttagt gatgtcacac tgccaagaaa agcttctagc gatacatata ctctacccgt
     2221 accgcaaacc gacacaggta gtcgaggcga gtagcctcag gtgagcgaga gaactctcgt
     2281 taaggaactc ggcaaaatgg ccccgtaact tcgggagaag gggcgctgat tttagatcag
     2341 ccgcagtgaa taggcccaag caactgttta tcaaaaacac agctctctgc taaatcgtaa
     2401 gatgatgtat agggggtgac gcctgcccgg tgctggaagg ttaagaggag tgcttagcgt
     2461 aagcgaaggt atgaattgaa gccccagtaa acggcggccg taactataac ggtcctaagg
     2521 tagcgaaatt ccttgtcggg taagttccga cccgcacgaa aggcgtaatg atttgggcac
     2581 tgtctcaacg agagactcgg tgaaatttta gtacctgtga agatgcaggt tacccgcgac
     2641 aggacggaaa gaccccatgg agctttactg cagtttgata ttgagtatct gtaccacatg
     2701 tacaggatag gtaggagcct atgataacgg gacgccagtt tcgtttgagg cgttgttggg
     2761 atactaccct tgtgttatgg ctactctaac ccagataggc aatccctatc ggagacagtg
     2821 tctgacgggc agtttgactg gggcggtcgc ctcctaaaag gtaacggagg cgcccaaagg
     2881 ttccctcaga ctggttggaa atcagtcgta gagtgtaaag gtataaggga gcttgactgc
     2941 gagagctaca actcgagcag ggacgaaagt cgggcttagt gatccggtgg ttccgtatgg
     3001 aagggccatc gctcaacgga taaaagctac cctggggata acaggcttat ctcccccaag
     3061 agttcacatc gacggggagg tttggcacct cgatgtcggc tcgtcgcatc ctggggctgt
     3121 agtcggtccc aagggttggg ctgttcgccc attaaagcgg cacgcgagct gggttcagaa
     3181 cgtcgtgaga cagttcggtc cctatccgtc gcgggcgtag gaaatttgag aggatctgct
     3241 cctagtacga gaggaccaga gtggacttac cgctggtgta ccagttgtct tgccaaaggc
     3301 atcgctgggt agctatgtag ggaagggata agcgctgaaa gcatctaagt gcgaagccca
     3361 cctcaagatg agatttccca tgattttata tcagtaagag ccctgagaga tgatcaggta
     3421 gataggttag gagtgtaatt gtggtgacac atttagcgga ctaatactaa tagctcgagg
     3481 acttatccaa aaagagatat tgacaacgtt acggtttctt gttagactat agatattcaa
     3541 ttttgagtga ataagactca atagttaagt gataattgcc taggagatac acctgttctc
     3601 atgccgaaca cagaagttaa gccctagaac gccggaagta gttgggggtt gccccctgt
//
LOCUS       DBJYSH010000140         3646 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.140, whole genome shotgun sequence.
ACCESSION   DBJYSH010000140 DBJYSH010000000
VERSION     DBJYSH010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 3646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3646
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.140"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            159..965
                     /locus_tag="ACUBV3_001857"
     CDS             159..965
                     /locus_tag="ACUBV3_001857"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HHU3848330.1"
                     /translation="MKITLHGVAETLLITLYIRAKDAMAKHPILNDQKSLAIVEQIEY
                     DFDKFDNSEASFYATLARIRVMDREIKKFIRENPNSQILSIGCGLDTRFERVDNGQIR
                     WYNLDLPEVMEIRKLFFEEHERVTNIAKSALDETWTREVNPQNAPFLIVSEGVLMFLK
                     EDDVETFLHILTNSFSQFMAQFDLCHKEMINKGKQHDTVKYMDTEFQFGITDGHEIVD
                     LDPKLKQINLINFTDEMSKFELGTLRSLLPTIRKFNNCLGVYEYKASEKK"
     gene            1360..1857
                     /gene="ybeY"
                     /locus_tag="ACUBV3_001858"
     CDS             1360..1857
                     /gene="ybeY"
                     /locus_tag="ACUBV3_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HHU3848331.1"
                     /translation="MYVEMIDETGQVSEDIKKQTLDLLEFAAQKTGKENKEMAVTFVT
                     NERSHELNLEYRDTDRPTDVISLEYKPEVDISFDEEDLAENPELAEMLEDFDSYIGEL
                     FISIDKAKEQAEEYGHSYEREMGFLAVHGFLHINGYDHYTPEEEKEMFSLQEEILTAY
                     GLKRQ"
     gene            1838..2236
                     /gene="dagK"
                     /locus_tag="ACUBV3_001859"
     CDS             1838..2236
                     /gene="dagK"
                     /locus_tag="ACUBV3_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000365222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HHU3848332.1"
                     /translation="MDLNDNNQKKWKNRTLTSSMEFAVTGIFTAFKEERNMRKHLVSA
                     ILVILAGLTFQVSMVEWLFLLLSIFLVITFEIINSAIENVVDLASNYHFSMLAKNAKD
                     MAAGAVLVVSLFAVLVGLIIFIPKILALLL"
     gene            2278..3177
                     /gene="era"
                     /locus_tag="ACUBV3_001860"
     CDS             2278..3177
                     /gene="era"
                     /locus_tag="ACUBV3_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012658024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HHU3848333.1"
                     /translation="MTFKSGFVAILGRPNVGKSTFLNHVMGQKIAIMSDKAQTTRNKI
                     MGIYTTETEQIVFIDTPGIHKPKTALGDFMVESAYSTLREVETVLFMVPADEKRGKGD
                     DMIIERLKAAKIPVILVINKIDKVHPDQLLEQIDDFRSQMDFKEVVPISALQGNNVPT
                     LIKLLTDNLEEGFQYFPEDQITDHPERFLVSEMVREKVLHLTQQEVPHSVAVVVESMK
                     RDEETDKVHIRATIMVERDSQKGIIIGKQGAMLKKIGKMARRDIELMLGDKVYLETWV
                     KVKKNWRDKKLDLADFGYNEKEY"
     gene            3414..>3646
                     /locus_tag="ACUBV3_001861"
     CDS             3414..>3646
                     /locus_tag="ACUBV3_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3848334.1"
                     /translation="MHNILRFLGIVIISAVILFSIGSFYDLSLMKNILLICWSFLFDL
                     LVFVFKQRQTTEVLTWYQVVKQFWLFIKCTILIP"
BASE COUNT         1262 a          482 c          712 g         1190 t
ORIGIN      
        1 gtttttttag aaagcataag aagtacagaa aaagaaaatt atggaatatt ggatggtggg
       61 gatatgcttt acttacggta ttggaatgat atacttaaag aaatccagtt ttagaaatcc
      121 aaatttctaa gtaaaatagc ataaaggaga gtagatgaat gaagataaca ttacacggcg
      181 ttgctgaaac gttattaatc accttatata ttagggctaa agatgcgatg gctaaacatc
      241 cgatacttaa tgatcaaaaa tccttagcaa ttgttgaaca gatagaatat gattttgata
      301 aattcgataa ttcagaagct tctttttatg caacattagc tagaattcgc gttatggata
      361 gagaaatcaa aaaatttatt agagaaaatc caaatagtca aatcctttca attggttgtg
      421 gacttgatac aaggtttgaa agagtcgata atggacaaat taggtggtat aaccttgatt
      481 tgccagaggt tatggagata agaaaattat tttttgaaga gcatgaaaga gttactaata
      541 tagcaaaatc agccctagat gaaacttgga cacgggaggt aaatccccaa aatgcccctt
      601 ttctaatcgt gtcagaaggt gttttaatgt ttctaaaaga agatgacgta gagacttttc
      661 ttcatatcct gacaaattca tttagccaat ttatggcaca atttgatttg tgtcataagg
      721 aaatgattaa taaaggaaag caacatgata cagtaaagta tatggataca gaatttcagt
      781 ttggtatcac agatggtcat gagattgtgg atttagaccc taaattaaag caaataaatc
      841 tgattaactt tacagatgag atgagcaaat ttgagttagg cacacttcgc tctttacttc
      901 caacaattcg taaatttaat aattgtttag gtgtgtacga atataaagca tctgagaaaa
      961 agtaacaaaa atctatttat ataaagagac attctaaatg taaatttgtc ataatcgtta
     1021 tagcatttgt agctttgttt atttaggaat tagttttacg taagttaggc gctctaaagt
     1081 ttgtattttg aagtaaatac taattcataa gtcagaattt tattgaaatg gattataagt
     1141 gtgtaatagt gattattagc tttctctccc ttttttaata ctcttatggt ataatgatgt
     1201 gaaagaatag cttgaaggaa ggaagagaac gcacttttat tttgagaact agttttagat
     1261 aaaagtgaaa aacttaggaa agtagatgtg tcgattattt agaattatgc attacacttt
     1321 atcttttcta aacgttttgg gtcgttctta ctatataaga tgtacgttga aatgattgat
     1381 gaaactggac aagtatcaga agatatcaaa aagcaaactc ttgatttgtt agagtttgca
     1441 gcacaaaaaa caggtaaaga gaataaagaa atggctgtaa cgtttgtcac taatgaacgt
     1501 agccatgaat tgaatttaga gtatcgagat accgatcgtc cgactgatgt tatcagttta
     1561 gaatataagc ctgaggttga tatttctttt gatgaagaag atctggctga aaatcctgaa
     1621 cttgctgaga tgctagaaga ttttgattct tatattggtg aactttttat ttctattgat
     1681 aaagctaaag aacaagcaga agagtacggt cactcttatg aacgtgaaat gggtttttta
     1741 gctgtacatg gttttttgca tattaacggt tatgatcatt atacacccga agaagaaaaa
     1801 gagatgttca gcttacagga agaaatttta actgcttatg gacttaaacg acaataatca
     1861 aaaaaaatgg aaaaatcgca ccttaacatc aagcatggaa tttgctgtta cagggatttt
     1921 tacagctttt aaagaagaaa gaaatatgcg taagcatttg gtttctgcca ttttagtaat
     1981 acttgctggt cttacttttc aagtatctat ggtggagtgg ttatttcttt tattgagtat
     2041 ttttttggta attacttttg aaattatcaa ttcagctatt gaaaatgtag ttgatttggc
     2101 aagtaattac catttctcga tgctggctaa aaatgctaaa gatatggcag caggagcggt
     2161 tttagttgtt tccttatttg ccgtattggt gggattgatt atttttatac ccaagatact
     2221 tgctttactt ttatgatgag aagtattacg aataaattga atggagaaag aaaagaaatg
     2281 acatttaagt caggatttgt agcaatttta ggtcgtccaa atgttggaaa atcaaccttc
     2341 ctaaaccatg ttatgggaca aaaaattgct atcatgagtg ataaagctca aacaacacgt
     2401 aataaaatta tggggattta tacaacagaa actgagcaaa ttgttttcat tgatacccca
     2461 ggaattcata aaccaaagac cgcccttggt gattttatgg ttgaatctgc ttatagtact
     2521 ttacgtgaag ttgaaacagt tcttttcatg gttcctgccg atgaaaagcg aggcaaaggc
     2581 gatgatatga ttattgaacg tcttaaggct gccaagattc ctgttatttt agtaatcaac
     2641 aaaattgata aggttcatcc agaccaactt ttggaacaga ttgatgactt ccgtagtcaa
     2701 atggatttta aagaagtagt gccaatttct gcccttcaag gaaataatgt gccaacccta
     2761 atcaagttat tgactgataa tcttgaagaa ggtttccaat atttcccaga agatcagatt
     2821 actgatcatc cggaacgatt tttagtatca gaaatggttc gtgagaaggt acttcattta
     2881 acgcaacaag aggttcctca ctcagtagct gttgttgtcg aatccatgaa acgtgatgaa
     2941 gaaactgata aagtgcatat tcgtgcaact atcatggttg aacgtgatag tcaaaaaggc
     3001 attatcattg gaaaacaagg tgctatgctc aagaaaatag gaaaaatggc gcgccgtgat
     3061 attgaactta tgttaggtga taaagtttat ctagagactt gggtaaaagt caagaaaaac
     3121 tggcgtgata aaaagcttga tttagctgat tttggctata atgaaaaaga gtattaatga
     3181 gtttattttg agatccgcaa gttagtgggg aagagttaga taagttcacc gtgagacttg
     3241 attcgcacag aaaaagcaat tcacgaggat aaaaccaact agtcataatt ttgagattat
     3301 attcacaaat taattaaagg gagccaaaca tgcttgttgg ccccttctta aataaaggag
     3361 agcacatgac tcaagattat atttgctacc tataaagtag aggaggtgag gatatgcata
     3421 atatattaag atttttaggc attgttatta tttctgcagt gattctattt agtataggta
     3481 gtttctacga tttatcatta atgaaaaata tactacttat ctgttggagt tttctctttg
     3541 atctattggt ttttgtcttc aaacaaagac aaacgacaga agtactcaca tggtatcaag
     3601 ttgttaagca attttggtta tttattaagt gtactattct tattcc
//
LOCUS       DBJYSH010000141         3433 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.141, whole genome shotgun sequence.
ACCESSION   DBJYSH010000141 DBJYSH010000000
VERSION     DBJYSH010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 3433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3433
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.141"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            354..1061
                     /locus_tag="ACUBV3_001862"
     CDS             354..1061
                     /locus_tag="ACUBV3_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HHU3848335.1"
                     /translation="MVCVDFIDQKVYSEAEGLFVSDLDLGIKGLIRARYPFALDSDFI
                     SYGNLSHYCMNYLDEIVQRANQKNEKIKYNVTTLMKEEEKPFNVEERLNKQATIGQRI
                     ADDVARFGGSWTFIIVFVSIMAIWMLVNIMKPFGIQFDPYPFILLNLALSTIAAIQAP
                     LIMMSQNRAADYDRLQARNDFNVNKTSELEIRLLHEKIDHMVQQDQFELLEIQKLQTE
                     MLVSLGNQLAQLKQLQK"
     gene            complement(1226..2311)
                     /locus_tag="ACUBV3_001863"
     CDS             complement(1226..2311)
                     /locus_tag="ACUBV3_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HHU3848336.1"
                     /translation="MTTNVMANYSYGEDCFKEIPSVLATYRIQSIAFVGGKRALDSSE
                     AEVRSILEEANIKVTGSFVYGTDSTQSNIDKLVANPQVQAADAILGFGGGKALDTAKM
                     VAKELGKNSFTIPTICSNCSAGTAIAVVYNDDHSFLRYGYPESPLHIFINTRIIAQAP
                     SKYFWAGIGDGISKAPEVERATLEAKTNKLPHTAVLGQAVALSSKEAFYQFGEQGLKD
                     VEANLASRAVEEIALDILISTGYASNLVNQPDFYYNSCHAHAFYYGTTAIQRQGEFLH
                     GVVVAFGVLVLHAYFNELEELEKVARFNKSLGLPTTLADVSLSEKDIPKIVEIAMTTN
                     EYKNTPFDPKMFAQAILAADAFGQTLA"
     gene            complement(2512..3051)
                     /locus_tag="ACUBV3_001864"
     CDS             complement(2512..3051)
                     /locus_tag="ACUBV3_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topology modulation protein"
                     /protein_id="HHU3848337.1"
                     /translation="MKIAIIGYSGSGKSTLARKLGNYYNCNVLHLDSIHFAPNWEERK
                     YDDMIDDVSNMLEKRTWIIEGNYKKLLYQERLADADEIIFFDFNRFNCLWRAFKRYCK
                     FRGKTRPDMANGCPEKLDFEFISWILKDGRSDKQKSNYKQVVEDYPQKIKILKHQRDL
                     DQYLKELPVASNTDPLKSL"
BASE COUNT         1100 a          550 c          625 g         1158 t
ORIGIN      
        1 ctcttaactt tcttactttt tagttggtcg tttattatta taataaaagt tacgtaccaa
       61 aacagaggtt aaactagatt tttaagccac cttctatact aagataaatt agttaattta
      121 agttttcttt aagtttatag acttagaata aagcgttatt ttgatgggta gcataaaatt
      181 aaggcataat atggatgtca gcgatttttt tagactaacg aaataacgct gtaaagctta
      241 ataggggtgt atcaaatact cacttatata gtaccaaaag cctatagcct gttggctagc
      301 ttgaccgtat tcaaatattg aataaatctt ggttgagaaa gggaggtata aaaatggtat
      361 gtgtcgattt tattgatcaa aaagtttata gtgaagcaga aggcctattt gtttccgatt
      421 tagacttagg cattaaaggt ttaataagag cacgttatcc ttttgctctg gatagtgact
      481 ttatttcgta tggaaattta agtcattatt gtatgaatta tcttgacgaa attgttcagc
      541 gagcgaatca gaaaaatgag aagataaaat ataatgtgac taccttgatg aaagaagaag
      601 agaagccctt caacgttgaa gagcgtttga acaagcaagc aactatcggt caacgaattg
      661 ctgatgatgt tgcacgtttt ggcggatctt ggacttttat tatcgttttt gtatccatta
      721 tggctatttg gatgttggtt aatatcatga agccttttgg tattcaattt gatccttacc
      781 catttatcct gttaaactta gctttatcaa cgattgctgc aatccaagca ccacttatta
      841 tgatgagtca aaatcgtgct gctgattatg atcgtttgca agctcgaaat gactttaatg
      901 tcaataaaac ctctgagtta gaaattagat tattgcacga aaagattgat catatggtac
      961 aacaagatca gtttgaattg ttagagattc aaaagttaca gacagaaatg ctagtatcat
     1021 tgggaaatca gttagcacaa ttgaaacaac tacaaaaata atcttttagc agttatttaa
     1081 aaatactgat acaatactaa gaatttaaat taatactgca acttcctata atggagagca
     1141 gtatttttac tttattaata acaaaaaccc cttagatgat gtgtctaagg gatttttgta
     1201 tcggaataat cgagttggtg aattattagg ctagtgtctg tccgaaagcg tctgctgcta
     1261 aaatagcttg agcaaacatt ttgggatcga aaggggtatt tttgtattca ttagtagtca
     1321 ttgcaatttc aacaattttt ggaatatctt tttctgataa agagacgtca gctagagttg
     1381 ttggcaaacc tagtgactta ttaaaacgag ctactttttc aagttcttct agttcgttga
     1441 aataagcatg taaaactaac acaccaaacg caactacaac gccatgtaag aattcacctt
     1501 gtctttgaat agcggttgta ccatagtaaa atgcatgcgc gtgacaagaa ttgtagtaga
     1561 aatcaggttg attaactaaa ttagaagcat agccagttga gattaagata tcaagcgcaa
     1621 tttcttcaac tgcacgcgaa gctaaattag cttcaacatc ttttagacct tgttcaccaa
     1681 attgataaaa agcttccttt gaagacagag cgactgcttg tcctaacact gcagtatgtg
     1741 gtagtttatt ggtcttagcc tctaaggtag cacgttctac ttcaggggct tttgaaatac
     1801 cgtccccaat accagcccaa aaatatttgc ttggtgcctg tgcaataatc cgtgtattga
     1861 taaaaatatg aagtggagac tcgggatagc cgtatcttaa gaaagaatgg tcatcattat
     1921 atacaactgc aatggcagta cctgcggagc aattagaaca aatagttggg atagtaaagc
     1981 tatttttacc taactctttt gctaccattt tagctgtgtc taaagctttt ccaccgccaa
     2041 aacctaagat agcatcagcg gcttgaactt gtgggttggc tactaacttg tcaatgttag
     2101 attgtgttga atccgttcca tagacaaagc ttcctgtgac tttaatattt gcttcctcta
     2161 aaatagagcg aacctcggct tctgagctgt ctagggcacg tttgccaccg acgaaagcaa
     2221 tgctttgaat acggtaagtt gccaaaacgc taggaatttc tttgaagcaa tcttctccgt
     2281 agctataatt tgccattaca tttgttgtca ttcagtgtcc tcctctattc caaaggtcga
     2341 ttagagacag ctattttcta aaagtgaata gttttttgtc tcttgaccaa gtgattatca
     2401 tattatagca gaatattcag aaaattggaa catgaaaaac aatttaattt tcaagaataa
     2461 gaatagaaaa ggtaaagcga gatatacgag taaatactgt cagtataata attacaagct
     2521 cttgagaggg tctgtattgg atgcaacagg gagttctttt aggtattgat caagatcgcg
     2581 ttggtgtttt agaatcttta ttttttgagg gtagtcctca acaacttgtt tatagtttga
     2641 tttttgtttg tcagagcgac cgtctttaag aatccaactg ataaattcaa aatctaattt
     2701 ttccgggcat ccattggcca tatcaggtct agtcttacca cgaaacttac agtaacgttt
     2761 gaatgctctc cacaagcagt tgaagcgatt aaagtcaaaa aaaataatct catctgcatc
     2821 tgctagacgt tcttgatata gcagtttttt gtagttacct tctataatcc atgttctttt
     2881 ttctaacatg ttgctgacat cgtcaatcat gtcatcatat tttctctctt cccaatttgg
     2941 ggcgaagtga atactatcaa gatgtaatac attgcaatta tagtaatttc ccaattttct
     3001 tgctaaagtg gacttacccg acccactata tccgattatt gctattttca tatactacct
     3061 ccgttaaatt gtttacttga agttaatgct gtacttagga tttacttatt ttataagggt
     3121 tattcacttt tttagattaa tatatttttg atataagtta tccatacaca tggtaacata
     3181 gttaccagta aatgtaatct agatatcaat actatggctg acgatattgt taacttttgg
     3241 ttcaaaaaat tggggtaaaa atcatcggag ggaactgcca aacaaactca cagtttaaaa
     3301 tactaagtaa taaaaagtct tgaacttatt atgtttaagg ctttttaagt gtctaaaata
     3361 cttggtctag tatgaatttc taaacagaaa gtaaaaataa tgagttttag tatggttttt
     3421 gggcataatg gaa
//
LOCUS       DBJYSH010000142         3426 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.142, whole genome shotgun sequence.
ACCESSION   DBJYSH010000142 DBJYSH010000000
VERSION     DBJYSH010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 3426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3426
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.142"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            complement(12..1160)
                     /gene="nagA"
                     /locus_tag="ACUBV3_001865"
     CDS             complement(12..1160)
                     /gene="nagA"
                     /locus_tag="ACUBV3_001865"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HHU3848338.1"
                     /translation="MTKYIKADRFFYADHVKENGYLEIKDNHFGKWIENISGQEEILD
                     YSGYQIAPGLVDTHIHGFAGADVMDCDSEGILRMSAGLLSTGVTSFLPTTLTSDTKRL
                     EEASKSVAAVAGKEQGAKIQGIYFEGPYFTEEYKGAQNPIYMRNPNLEEFAQWQKAAK
                     GLITKIALAPEREGVEEFVSAITKQGVTVALGHSNGTYKEAKKAVKAGASVWVHAYNG
                     MRGLTHREPGMVGAVYNLPNTYAELICDGHHVDPVACDILMTQKGHNHVALITDCMAA
                     GGAPDGDYMLGELPVVVSNGTARLKDGGNLAGSILKLKDGLKNVVEWGIATPEEAVRM
                     ASYVPAVSVGIDNVCGQIKEGYAADFIVLDKHLDLVATYLDGEKRWQS"
     gene            complement(1438..2145)
                     /locus_tag="ACUBV3_001866"
     CDS             complement(1438..2145)
                     /locus_tag="ACUBV3_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001871310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HHU3848339.1"
                     /translation="MKIDKKEFLALIASIILLIFASVTFFLFKDHGTTQMDTVESSVN
                     HVSDSQLTEAQDMLDKFEKKPSEKLLKDVELALNKLSNSSKKEALQKRFKKAKDKYLK
                     DEADKKATKDATDLVEILEQAPSEENVLKAEAAVNKLTVKESKEALQKRIDTVKTQYG
                     LIGNQTPSSSVAETTEQGTANPASQDTSSYVNQNVAPTYEQPQTNNTPVTPGVNNTVP
                     TPGTGTAPATNGTGVAQ"
     gene            2337..2648
                     /locus_tag="ACUBV3_001867"
     CDS             2337..2648
                     /locus_tag="ACUBV3_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HHU3848340.1"
                     /translation="MIPSQMLKGLLEGAILKIIQQEETYAYEISKKLNSYGFGEISEG
                     TIYPIILRLQSNKYIKGQQKPSPQGPKRKYYTLTNEGVEQLVQFKQNWESLSKALNKL
                     L"
     gene            2669..3352
                     /locus_tag="ACUBV3_001868"
     CDS             2669..3352
                     /locus_tag="ACUBV3_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3848341.1"
                     /translation="MDKLKKLQKENTLLEGRIDNSNNQTYTDMIVYLRGASISPYHQE
                     LIRNDIVNMLLEAQERQASLVSVFGEDRHDFINQVIKSTPKISKKEETLQRWDLAILL
                     LTIQMIIFLGGYLITEALQQSVPDLIPITLLDVLFAIFISIIAVKIADTIIYATYNFD
                     KSKEKKYFFRYMFLILSLIIAYILIGKYCHLPFINIPLWIYLIILGLSFSLHIIVKKY
                     LNKHYYIEF"
BASE COUNT         1091 a          687 c          507 g         1141 t
ORIGIN      
        1 aactagttct ttcaagattg ccaacgtttt tctccatcaa ggtatgttgc aactaaatct
       61 agatgtttat ctaatacaat aaaatccgct gcatagcctt cttttatctg tccacagaca
      121 ttatcaatgc ctacagacac tgctggaaca taactcgcca tacgcactgc ctcttccgga
      181 gtagcaattc cccattctac aacatttttt aatccatctt tgagcttaag gatagaacca
      241 gctaaattcc ctccatcttt caaacgcgca gttccattag acacaaccac gggcaattcc
      301 cctaacatat aatctccatc aggggcacct ccagctgcca tacaatctgt tattaatgcc
      361 acatgattat ggcctttttg tgtcattaaa atgtcacaag caacaggatc aacgtgatga
      421 ccatcgcaaa tgagttcggc ataagtatta ggtaaattat aaaccgcacc aaccatacct
      481 ggttctcggt gtgtcaaacc acgcatacca ttatacgcat gtacccaaac tgaggcaccc
      541 gccttcactg cctttttagc ttctttatat gttccattag aatgacctaa agctaccgta
      601 acaccttgct ttgtgattgc tgagacaaat tcctcaactc cctcacgttc tggtgccaaa
      661 gcaatttttg taatcaagcc ttttgcagcc ttctgccatt gagcaaactc ttccaagttt
      721 ggatttctca tataaatcgg attttgagct cctttatatt cctcagtaaa atatggtccc
      781 tcaaaataga tgccttgaat ttttgctcct tgttctttgc ccgctactgc cgcaactgat
      841 ttagatgcct cttctagtcg ctttgtatct gaagttaggg ttgtcggcaa gaaagatgtg
      901 actccagtag atagcaagcc agcactcatt cgcaaaattc cttctgaatc acaatccata
      961 acatcagcgc cagcaaaacc atgaatatgt gtatcaacta aaccaggtgc tatttggtaa
     1021 cctgaataat ctaaaatctc ctcttgtcca gaaatatttt ctatccattt accaaagtga
     1081 ttatctttta tttctaaata accgttttcc tttacatgat cagcataaaa gaatcggtca
     1141 gccttgatgt acttcgtcat cttcttcccc ccattcactc tcattataac gctttaaggt
     1201 atttttgtaa atggtatata cctatttttt gaaaagtcat gatttattct aagaaagaac
     1261 tatacacttg ttataaacca cttcacttgg ttgtactttc aaggttttaa ttgctgttgc
     1321 tcataattct tttccttctc caaaactatc aattgctaat tctcttatag tttttgatga
     1381 gaatggctaa ttaaaaagca tagcaaaaaa gtctagtaaa actagacttt cagggtttta
     1441 ctgagcaaca cccgtcccat tagtagcagg tgcagtacca gttcctggag tcggaacagt
     1501 attgttaacc cctggagtaa ctggtgtatt attcgtttgc ggttgctcat aagttggtgc
     1561 tacattctga ttaacgtaac tagaagtatc ctgactagca ggattagctg tcccttgttc
     1621 tgtagtttca gctactgaac tagagggagt ttgattccca atcaaaccat attgtgtctt
     1681 aacagtatca atacgttttt gtaaagcttc tttagattct ttgacagtta acttattgac
     1741 tgctgcttca gcttttaaca cattttcttc tgaaggggct tgttctagaa tttctactaa
     1801 atctgtagca tctttggtag ctttcttatc tgcttcgtct ttaagatact tatcttttgc
     1861 tttcttaaaa cgtttttgca aagcttcttt tttagaagaa ttggacaatt tatttaaagc
     1921 aagttcgaca tccttcaata atttttctga aggtttcttc tcaaatttat ctaacatatc
     1981 ttgtgcctca gttaattgag aatcactgac atggttaaca ctgctttcta cagtatccat
     2041 ttgagtagtg ccatgatctt taaataagaa aaacgtaaca gaagcaaaaa ttaataaaat
     2101 aatactagca ataagtgcta aaaattcttt tttatctatt ttcatactca ctcctaaagt
     2161 ttcttcacat tattataaca taaactaaag gacataaaca aacctatttt agaagaatca
     2221 tcgtctatca ctcttatttt ctaccggtaa cttagttata tttgacatct caactacttt
     2281 gtattacaat gtagctattc cggtaacttg tattacaata tagacaagga tataatatga
     2341 ttccatcaca aatgctaaaa ggattacttg aaggagctat cctaaaaatc atacagcaag
     2401 aggagactta tgcttatgaa atctcaaaaa aattaaattc ttatggtttt ggggaaattt
     2461 ccgaagggac aatctacccc attatcttaa ggctacaaag caataaatat ataaaaggac
     2521 aacaaaagcc ttcccctcaa ggacctaaac gtaaatatta tacgttaacc aatgaaggcg
     2581 tggagcaatt agtccaattt aaacagaatt gggagtcact tagtaaagca ttaaacaaac
     2641 tactctaaaa attggagagg aaactagcat ggataaattg aaaaaattac aaaaagaaaa
     2701 tacactatta gaaggaagaa ttgataactc taataaccaa acctatacag atatgattgt
     2761 ctatttgcga ggtgctagca ttagccccta ccaccaggag ctcattcgaa atgatattgt
     2821 caatatgtta ttagaagcac aagagcgaca agcaagttta gttagtgtat ttggagaaga
     2881 taggcacgac tttataaacc aagttattaa atctacacct aaaatctcta agaaagaaga
     2941 aacattacaa cgttgggatt tagccatttt actactaact atccaaatga ttatcttttt
     3001 aggaggatat ctcattactg aagcacttca acagtcagta ccagatctaa tccctattac
     3061 tctattagat gttctttttg caatctttat cagtattatt gctgtcaaga tagctgatac
     3121 cataatatat gctacatata acttcgataa atcgaaagaa aagaaatatt tctttcgata
     3181 tatgtttcta attctttcgc ttatcatagc ttatatcctt ataggaaaat attgtcatct
     3241 tcctttcatt aatatccccc tttggattta tttaattata ctgggactta gcttctctct
     3301 tcatatcatt gttaaaaaat acctgaataa acattattat attgaatttt aaaagaggta
     3361 taactatata aaaaataaaa cgacaaggat attcttagtc gttttattct gtgcccattt
     3421 tcacga
//
LOCUS       DBJYSH010000143         3376 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.143, whole genome shotgun sequence.
ACCESSION   DBJYSH010000143 DBJYSH010000000
VERSION     DBJYSH010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 3376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3376
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.143"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            52..753
                     /locus_tag="ACUBV3_001869"
     CDS             52..753
                     /locus_tag="ACUBV3_001869"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HHU3848342.1"
                     /translation="MSYIEMKHSYKRYQTGETEIVANNDISFSIERGELVVILGASGA
                     GKSTVLNILGGMDSNSEGEVLIDGKNIANYTIRELTRYRRYDVGFVFQFYNLVPNLTA
                     LENVELASEIVPKALDAQQALENVGLGHRINHFPAQLSGGEQQRVAIARAIAKKPKLL
                     LCDEPTGALDYQTGKQVLAILQKMAQSKETTVIIVTHNTALAPIANRVIHMHDSKISD
                     IVINENPSDIQNIEY"
     gene            765..>3376
                     /locus_tag="ACUBV3_001870"
     CDS             765..>3376
                     /locus_tag="ACUBV3_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000520608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HHU3848343.1"
                     /translation="MGKTFWKDIYRSITTSKGRFSSILLLMMLGSFAFIGLKVSAPNM
                     QRTAQNYLAHHHVMDITVFNSWGLDKHDQTVLESLKGSQVEFSYFVDTTPQQNSKSYR
                     LYSNTKTISTFDLVKGRLPLNKSEIALSFQERKKYAIGDKINFKQDKNKLFSNTGPLT
                     IVGFVNSTEIWSKTNLGSSQTGDGDLDSYGVLDKTAFHSPVYTMARVTFKDLRLINPF
                     SISYKEKVAKYQEKVSRKLNIHNKIRYTKTKKESLRKIDEEEKSLLKAQKQINRLDND
                     SLAMPLSQRQAIQMKIKQDRLSLLKRTKELLKLRHNTQIMESPQIIVYNRTTFPGGQG
                     YNTFDSSTNSTSKISNLFPIILYLVAALVTLTTMTRFVEEERTNAGILKALGYSDRQV
                     IFKFIIYGFIAGTLGTTLGIIGGHYLLPRIISDIISKDLTIPNTQYHLFLNYSLLAFV
                     FSLLSIVLPVFVITRRELKEKAAFLLLPKPPAKGSKIALEYINWIWKKLSFTQKVTAR
                     NIFRYKQRMIMTIFGVAGSVALLFSGLGIQSSLKQTVNEHFGRIMPYDILLTYNTNAS
                     PPKILELLSKDSKIDKYQPIHLENLDESIPGQINKQSISLFITDKKQLLPFIYLQEAT
                     TNKSLHLNNKGIIISKKLAQFYHVNTGDFIHLSHSQTLPSRKLKITGVVNANVGHYIF
                     MTKQYYRTIFKKEAKDNAFLVKLTKHKIANNLAEKLLEINGVESLTQNALQLASVEAV
                     VRSLDGSMTILVVVSLLLAIVILYNLTNINLAERKRELSTIKVLGFYNEEVTLYIYRE
                     TIILSTIGVILGTISGTYLHRQMMLLIGSDQILFGEKVSPTTFIIPISVVVIILISLG
                     FIVNHQLKKLNMLDA"
BASE COUNT         1196 a          591 c          548 g         1041 t
ORIGIN      
        1 ttaataaaaa agtgctataa tataggtgta ttcatagtgt gaggtgccgt tatgtcctat
       61 attgaaatga agcattccta taagcgttat caaacaggtg aaaccgaaat tgttgctaac
      121 aatgatatta gtttcagtat tgaaagaggt gagttggtgg ttattcttgg tgcatccggc
      181 gctggcaaat ctactgttct caatatacta ggagggatgg atagtaatag tgaaggtgaa
      241 gttttaattg atggtaagaa tattgctaat tataccatca gggaattgac tcgttaccgt
      301 agatatgatg tcgggtttgt ttttcaattc tataacttag tccctaatct aacagcacta
      361 gagaatgtag aattggcttc agaaattgtt ccaaaggcat tagatgccca acaggcatta
      421 gaaaacgtag gtttgggaca ccgtatcaat cattttccag cacagctttc cggtggcgaa
      481 cagcaacgcg ttgcaatagc acgagctatt gccaaaaaac ctaaactgtt actttgtgat
      541 gaacctactg gtgctttaga ttaccaaact ggaaaacaag tactagccat cctgcaaaag
      601 atggcgcaat caaaagaaac aactgtgatc attgttactc ataatactgc cttagcgcca
      661 attgcaaatc gcgtgatcca tatgcatgat tcaaaaatca gtgatattgt aataaacgag
      721 aatccaagtg atattcagaa catagaatat tagaggttat acagatggga aagacatttt
      781 ggaaagatat ctatcgatca attacgactt caaaaggtag attttcatca atcctcttac
      841 ttatgatgtt aggctcattt gcttttatag gactgaaggt ctcagcacct aatatgcaaa
      901 gaacagcaca aaattacctt gctcatcatc atgttatgga tattaccgtt ttcaattctt
      961 ggggacttga caaacatgat caaactgttt tagaaagctt aaaaggatca caagttgaat
     1021 tttcttactt tgtagataca acccctcaac agaatagtaa atcttatcga ttgtattcca
     1081 atactaaaac gatatcaacc tttgatcttg ttaagggacg tctcccttta aataaatcag
     1141 aaattgctct ctcatttcag gaacgaaaaa aatatgcaat aggtgataaa attaatttta
     1201 aacaagataa aaacaaactg ttttcaaata caggtcctct aactattgta ggttttgtta
     1261 attctaccga gatttggtct aagacaaacc ttggtagttc tcaaacaggg gacggcgact
     1321 tagatagtta tggcgtatta gataagacag catttcactc tccagtatat acaatggcaa
     1381 gggtaacttt taaagatctt aggttgatta accctttctc aataagttac aaagaaaaag
     1441 tagcgaaata ccaagaaaag gtatcgcgaa aattaaatat tcacaataaa ataagatata
     1501 ccaaaacaaa aaaagagagc ctacgtaaaa tcgatgagga ggaaaaaagc ctactaaaag
     1561 ctcaaaaaca aatcaatcgg ttagataatg atagcctagc gatgccacta tctcaaagac
     1621 aagctattca aatgaaaatt aaacaagacc gactatctct tttgaaacga acaaaagagc
     1681 ttctaaaatt gagacataac acccaaataa tggaatcgcc tcaaattatt gtctacaacc
     1741 gtactacctt tcctggagga caaggatata acacttttga ctccagtaca aatagcactt
     1801 ctaaaatcag taatcttttc cccattattt tatatttagt cgcagcatta gtaaccttaa
     1861 caacaatgac taggttcgtc gaggaagaaa gaaccaacgc aggcatatta aaagcactag
     1921 gctatagtga ccgccaagtt atctttaaat ttattattta tggttttata gcaggaacat
     1981 tagggactac tttgggtatc attggagggc attatctttt acctcgtatt atttctgata
     2041 tcatttccaa agacctgact attccaaaca cacagtatca tcttttctta aattacagtt
     2101 tactagcctt tgttttttca ttattaagca ttgttcttcc agtttttgtt atcactcgac
     2161 gtgagttgaa agaaaaagca gcatttttat tattaccaaa accaccagct aaaggatcta
     2221 aaatagcttt agagtatatc aattggattt ggaaaaaact gtccttcaca caaaaagtaa
     2281 ctgctagaaa tatttttcgt tacaaacaaa ggatgattat gaccatattt ggagttgctg
     2341 gatcagtagc gctcttattt tcaggtctag gaatacaatc ctccttaaag caaaccgtca
     2401 atgaacattt cggtcgtatt atgccctatg atatattgct aacatataac acaaatgctt
     2461 ctcccccaaa aatacttgaa ctactttcaa aagattcaaa aatagacaaa taccaaccaa
     2521 tacaccttga aaatcttgac gaatccattc ctggacaaat taataaacag tcaatttccc
     2581 tattcataac agacaaaaaa caattactac cttttattta tttacaagaa gcaacaacta
     2641 ataagtcatt gcacttgaat aataaaggga ttattatttc taaaaaatta gctcaatttt
     2701 atcatgttaa taccggtgat tttatccatt tatctcattc acaaacactt ccttctagaa
     2761 aattaaaaat aacaggagtt gtcaatgcga atgttggtca ctatattttt atgacaaaac
     2821 aatactatcg aactattttt aagaaagaag ctaaagataa cgcttttttg gttaagttaa
     2881 ccaaacataa aatcgcaaat aacttagcag aaaaactttt agaaattaat ggagttgagt
     2941 ctcttacgca aaatgctctt cagctggcta gtgtagaagc cgttgtgcgt tctttagatg
     3001 gctctatgac tattttagtt gtcgtatctt tattgttagc cattgtaatt ctctataatc
     3061 tcactaatat taatttagca gaacgtaagc gggagctatc aactataaag gtattaggtt
     3121 tttataatga agaagtaact ttatacattt accgcgaaac cattatttta tcaaccatcg
     3181 gtgtgattct aggcaccatt agcggtactt atttacaccg tcaaatgatg ttgctaattg
     3241 gttcagatca aatacttttt ggtgaaaaag tatcaccaac tacatttata ataccaataa
     3301 gcgtagtagt catcattcta ataagtctag gttttatagt taaccatcaa ttaaaaaaac
     3361 tcaatatgtt agatgc
//
LOCUS       DBJYSH010000144         3374 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.144, whole genome shotgun sequence.
ACCESSION   DBJYSH010000144 DBJYSH010000000
VERSION     DBJYSH010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 3374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3374
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.144"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            140..1150
                     /locus_tag="ACUBV3_001871"
     CDS             140..1150
                     /locus_tag="ACUBV3_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HHU3848344.1"
                     /translation="MGIGIIIASHGKFAEGIHQSGSMIFGEQEKVQVVTFMPNEGPDD
                     LYGHFNNAIAQFDADDEVLVLADLWSGSPFNQASRVMGENPERKMAIITGLNLPMLIQ
                     AYTERMMDANAGVEQVAANIIKESKEGIKALPEELNPVVEATPVAGVPADVPAEVKQS
                     GSIPEGTVIGDGKLKINLARIDTRLLHGQVATAWTPASKANRIIVASDEVSKDELRKQ
                     LIKQAAPGGVKANVVPISKLIEVAKDPRFGNTRALILFETVQDALRAVEGGVEIPELN
                     VGSMAHSTGKTMVNNVLSMDKDDVAAFEKLRDLGVSFDVRKVPNDAKKNLFDLINKAN
                     VK"
     gene            1183..1995
                     /locus_tag="ACUBV3_001872"
     CDS             1183..1995
                     /locus_tag="ACUBV3_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HHU3848345.1"
                     /translation="MADISIISMILVVIVAFFAGLEGILDQFQIHQPLVACTLIGLVT
                     GHLEAGIILGGSLQMVALAWANIGAAVAPDAALASVAAAIILVKSGDFTQKGINFAFS
                     TAVPLAIAGLFLTMIVRTISTALVHAGDKAASEGNFAAIERFHFIALLLQGLRIAFPA
                     ALLLAIPSSSVQSILEAMPDWLNGGMQVGGAMVVAVGYAMVINMMATREVWPFFALGF
                     ALAALNQLTLIAMGTIGVAIALIYISLSKMGGSKGTSNAGSNDPIGDILEDY"
     gene            2010..2921
                     /locus_tag="ACUBV3_001873"
     CDS             2010..2921
                     /locus_tag="ACUBV3_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000139230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HHU3848346.1"
                     /translation="MTEQIKLSKSDRQKVWWRSQFLQGSWNYERMQNMGWAYALIPAL
                     KKLYTTKEDRAAALERHMEFFNTHPYVAAPIIGVTLALEEEKASGTPVEDKAIQGVKI
                     GMMGPLAGIGDPVFWFTVRPILGALGASLASAGNILGPIIFFVGWNLIRMSFLWYTQE
                     LGYKSGKEITKDMSGGILQDITKGASILGMFILAVLVKRWVAINFTVDLPKKTLSEGA
                     YINFPKDHVSGQQLHDILGQVQSGLSLDKMQPQTLQGQLDSLIPGLAGLLLTFFCMWL
                     LKKKVSPITIIIGLFIVGILARLAGVM"
     gene            3040..>3374
                     /locus_tag="ACUBV3_001874"
     CDS             3040..>3374
                     /locus_tag="ACUBV3_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HHU3848347.1"
                     /translation="MAQSLNKTVEFQTTGVSYLGMGNKVGKFLVGDQALEFYNDKNVN
                     DYIQIPWTSINQIGANVSRKKISRHFEVFTDQGKFLFASKDSGTILKHARRHIGDDKV
                     VKLPTLIQT"
BASE COUNT          997 a          640 c          680 g         1057 t
ORIGIN      
        1 gatttttttc tgaaaacgtt ttaaaatata tctgttctat aatttcgttg aaatgaaaaa
       61 gaatggccaa ttttacgatt ggaaggagat tattcttctt tatgtcaacg aaaaaatttt
      121 ttaaggagga agaacaagaa tgggtatcgg tattattatt gccagccatg gtaaatttgc
      181 tgaaggtatt catcaatcag gttcaatgat ctttggtgaa caagaaaaag ttcaagttgt
      241 aactttcatg ccaaatgaag gaccagatga tttgtatgga cacttcaaca atgctatcgc
      301 acaatttgat gctgatgatg aggtgcttgt gttagctgac ctttggagtg ggtcaccatt
      361 taaccaagca agccgtgtta tgggagaaaa tcctgaacgt aaaatggcta tcatcactgg
      421 tctcaatttg ccaatgctta tccaagccta tactgagcgt atgatggacg ctaatgcagg
      481 tgttgaacaa gttgccgcaa atattattaa agagtctaag gaaggtatca aagcccttcc
      541 agaagaactt aatcctgttg tagaagctac tcctgtagca ggtgttcctg cagatgttcc
      601 tgcggaagtt aaacaaagtg gatctatccc tgaaggaact gttattggag atggcaagct
      661 taaaattaat cttgctcgta ttgacacacg tcttttacat ggtcaagttg caactgcatg
      721 gactccagca tcaaaagcaa accgtattat cgttgcttct gatgaagttt ctaaagatga
      781 acttcgtaaa caattgatta agcaagctgc tcctggagga gtcaaagcaa acgttgtccc
      841 aattagtaaa ttaattgaag tagctaaaga cccacgtttc ggaaacacac gtgccttgat
      901 cttatttgaa actgtacaag atgcactccg tgcagttgaa ggtggtgtgg aaatccctga
      961 attgaacgtt ggttcaatgg ctcactcaac tggtaaaaca atggtaaaca acgttctttc
     1021 aatggataaa gacgatgttg ctgcctttga aaaattacgt gacttaggtg tatcatttga
     1081 tgtacgtaaa gtaccaaatg acgctaaaaa gaatttgttt gatttaatca acaaagcaaa
     1141 cgtaaaataa cttattatct ctgaaaacga aaggatatca aaatggcgga tatttcaatt
     1201 atctcaatga ttttggtcgt tatcgttgcc ttctttgctg gtcttgaagg tatcctcgac
     1261 caattccaaa tccatcaacc tcttgttgct tgtactttaa tcggtcttgt aactggtcac
     1321 ctcgaagcgg gtattattct tggtggtagt ctccaaatgg ttgctcttgc ttgggcaaat
     1381 atcggagctg ccgtagcacc agacgctgca cttgcctctg ttgctgctgc tattattttg
     1441 gttaaaagtg gcgacttcac tcaaaaaggt atcaactttg ccttctctac tgctgtacct
     1501 ctagctattg ctggactttt ccttacaatg attgttcgta caatctcaac tgcacttgtt
     1561 cacgctggtg acaaagctgc ttctgaagga aacttcgcag caattgaacg ttttcacttt
     1621 attgccctcc ttctccaagg attacgtatt gcattcccag ctgcgcttct tttagctatt
     1681 ccatcttctt cagtacaaag tattcttgaa gcaatgcctg attggttaaa tggtggtatg
     1741 caagttggtg gtgccatggt tgttgccgtt ggttacgcta tggttatcaa tatgatggct
     1801 actcgcgaag tttggccttt ctttgctctt ggatttgctc tagcagcttt gaaccagtta
     1861 actcttattg caatgggtac tattggtgtt gctatcgccc tcatctacat cagtttatca
     1921 aaaatgggtg gaagcaaagg tacttctaac gcaggttcta acgatcctat cggcgatatc
     1981 ttagaggact actagaaagg agctaaacaa tgactgaaca aattaaatta tcaaaatcag
     2041 atcgtcaaaa agtttggtgg cgttcacaat tccttcaagg ttcttggaac tatgagcgta
     2101 tgcaaaatat ggggtgggcc tatgctctta tcccagcatt gaaaaaactt tacactacta
     2161 aagaagatcg tgctgctgcg cttgagcgcc atatggaatt ctttaacaca catccatacg
     2221 ttgctgctcc aattattggt gtaacccttg ctcttgagga agaaaaagca agcggtacac
     2281 ctgtcgaaga caaagcaatt caaggggtta aaatcggtat gatgggacct cttgctggta
     2341 ttggtgatcc agtgttctgg tttactgtac gtcctatctt aggtgctctt ggtgcttcac
     2401 ttgcctctgc tggtaatatc ctcggaccaa tcatcttctt tgttggttgg aacctcattc
     2461 gtatgtcatt cttatggtac acacaagagt taggttacaa atcaggtaaa gaaattacta
     2521 aagatatgtc tggcggtatc ctccaagata ttactaaagg tgcttcaatc cttggtatgt
     2581 tcatcctcgc tgtccttgtt aagcgttggg tagcaattaa ctttactgta gaccttccta
     2641 aaaagacatt gtcagaaggg gcatacatca atttccctaa agaccatgtt agtggacaac
     2701 aattgcatga tatcctagga caagttcaga gtggcttaag ccttgataaa atgcaaccac
     2761 aaacacttca aggacaactt gactcactta ttcctggttt agctggatta cttcttacat
     2821 tcttctgtat gtggttactt aagaagaaag tatcaccaat tacaattatc atcggtttgt
     2881 ttatcgttgg tattctagct cgccttgcag gtgtgatgta ataaaactaa gagatggtta
     2941 caatagtatt ccatctctta tattttatta ccaaaaatcc acttagagtc ccactattat
     3001 gatataatca aactactata aattaaaaag ggattagtca tggcacaatc attaaacaaa
     3061 actgttgaat ttcaaacaac aggagtttcg tatttaggta tggggaataa agttggaaaa
     3121 tttttagttg gtgaccaggc attagaattc tataacgata aaaatgtcaa cgactatatt
     3181 caaattccat ggacatcaat caatcaaatt ggagctaatg ttagtcgaaa aaaaattagt
     3241 cgtcactttg aggtctttac agaccaagga aaatttttat tcgcttctaa agactctgga
     3301 acgatattaa agcatgcgag acgacatatt ggagacgata aagttgttaa gcttcctacc
     3361 ctcatccaga ccat
//
LOCUS       DBJYSH010000145         3360 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.145, whole genome shotgun sequence.
ACCESSION   DBJYSH010000145 DBJYSH010000000
VERSION     DBJYSH010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 3360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3360
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.145"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            <1..1665
                     /locus_tag="ACUBV3_001875"
     CDS             <1..1665
                     /locus_tag="ACUBV3_001875"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HHU3848348.1"
                     /translation="TDIEIAQSVALKPIAEIVEQVGIGFDDIELYGKYKAKLSFDKIE
                     AVRSQKVGKLILVTAINPTPAGEGKSTMSIGLADALNKIGKKTMIALREPSLGPVMGI
                     KGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELDIDQRRVI
                     WKRVVDLNDRALRQVIVGLGSPVNGIPREDGFDITVASEIMAILCLATDLSDLKKRLS
                     NIVVAYSRDRKPIYVKDLKIEGALTLILKDAIKPNLVQTIYGTPALVHGGPFANIAHG
                     CNSVLATSTALRLADYVVTEAGFGADLGAEKFLDIKTPNLPTSPDAIVIVATLRALKM
                     HGGVSKEDLSQENVEAVKRGFTNLERHVNNMRQYGVPVVVAINQFTADTESEIATLKT
                     LCSNIDVAVELASVWEDGADGGLELAQTVANVIETQSSNYKRLYNDEDTIEEKIKKIV
                     TKIYGGNKVHFGPKAQIQLKEFSDNGWDKMPICMAKTQYSFSDNPNLFGAPTDFDITV
                     REFVPKTGAGFIVALTGDVLTMPGLPKKTAALNMDVLEDGTAVGLF"
     gene            1784..>3360
                     /locus_tag="ACUBV3_001876"
     CDS             1784..>3360
                     /locus_tag="ACUBV3_001876"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HHU3848349.1"
                     /translation="MIWRGEKLISNKVKIVRLLNKSKKSLLRGIFSRTTVIAILLILQ
                     LLFLLASYSWLEQYRVWLATVEHILTIGAVLYLVNSEMDALSRVTWLILVMIAPLLGA
                     MFLMYTKFDWGYRGLKQRLETLIDESQIYLEDDPETLNQLKSSTSTTYHLVQYFEKAH
                     GNFPVYRNTDVTFLPTGEAFFEKMKEELLKAKKYIFLEFFIIDEGIMWGEILSILEQK
                     VEEGVEVRILYDGMIEITKLSFDYTKRLEKIGIKAKAFSPISPFISTYYNYRDHRKIV
                     VIDGVVGMTGGVNLADEYINHIELFGHWKDSGIMLKGKAVDSFLLLFLQMWSITEEKM
                     LVAPYLGVHDDLVENEGYVIPYGDSPLDTDKVGENVYIDILNHAREYVYIMTPYLILD
                     SELEHAIQFAAERGVDVRIIMPGIPDKPIPYALAKTYYQALIKSGVKIYEYTPGFVHS
                     KIFLSDNTKAVVGTINLDYRSLYHHFECAVYLYKVDAIQDIYRDYMDTLNKSRLVSLK
                     DINNIPKFQKVIGIVTKT"
BASE COUNT         1094 a          504 c          694 g         1068 t
ORIGIN      
        1 acagatattg aaatagcgca aagcgtcgcc ctaaagccta ttgctgagat tgttgagcaa
       61 gtaggaattg gttttgatga tatagaattg tatggtaaat acaaagcaaa attatctttt
      121 gataagattg aagctgtaag atctcaaaaa gtaggaaagt taattttagt aactgctatt
      181 aatccgacac cagcaggtga gggaaaatcc actatgagca ttggattagc tgatgcttta
      241 aataaaattg gtaaaaaaac gatgattgcg cttcgcgaac cttctttagg tcctgtaatg
      301 ggtattaaag gaggagctgc tggaggtgga tacgcacaag tactaccaat ggaagatatt
      361 aatttgcatt ttactggtga tatgcacgca atcacaactg ctaataatgc tttatcagct
      421 ctacttgata atcatattca tcaaggcaat gaattagata ttgaccaacg tcgagtgatt
      481 tggaagcgtg tagttgatct taatgatcgt gctcttcgtc aggtgatcgt tggcttagga
      541 agccctgtca atgggatacc acgtgaagat ggctttgata ttactgttgc ttctgaaata
      601 atggctattc tatgtttagc tactgaccta tcagatttaa agaaacgcct gtcaaatatt
      661 gttgtggctt actcaaggga ccgtaaacca atctatgtta aagatttgaa gattgaagga
      721 gctctcacac ttatcctaaa agatgctatc aaacctaatt tggttcaaac catttatggg
      781 acaccggctc ttgtccatgg gggaccattt gctaatattg cacatggatg taattctgtc
      841 ttagcgactt caacagctct acgcttagca gattacgttg ttactgaagc tggttttgga
      901 gcagacttgg gcgctgaaaa gtttcttgat ataaaaacgc caaaccttcc gacttctccc
      961 gatgcgattg ttattgttgc tacattgcgt gcattgaaaa tgcatggagg tgtttcaaaa
     1021 gaagatctct cacaagagaa cgtcgaagca gttaaaagag gttttacaaa tctcgagcgt
     1081 cacgttaaca atatgcgtca atatggtgtt ccggtagttg tagctatcaa tcagtttact
     1141 gcagatacag aaagtgaaat tgcaactctt aaaaccttat gtagtaatat tgatgtggca
     1201 gttgaattag caagtgtgtg ggaagatgga gcagacggtg gccttgaact tgcacagact
     1261 gttgctaatg tgattgaaac acaatcatcc aattacaaac gcttatataa tgatgaagac
     1321 actattgaag aaaaaattaa aaaaattgtt actaaaatat atggtggtaa taaagttcat
     1381 tttggaccta aggcacaaat acaattaaaa gagtttagtg acaatggctg ggacaagatg
     1441 cctatttgta tggcaaagac acaatatagc ttttctgata atccaaattt atttggtgct
     1501 ccaactgact ttgatataac tgttcgtgaa tttgttccaa aaacaggagc tggttttata
     1561 gttgctctta ctggagacgt attgacaatg cctggtttac ctaaaaaaac tgcagctctc
     1621 aatatggatg ttttagaaga cggtacagcc gttggattat tttaaataat aaagaggtag
     1681 ggcttgtgat gctagcctct ttttattggg gattagattt taactagaga taatttagaa
     1741 taatagaatg ctattcaagt gacagtatgc tataataaaa tgattgattt ggagggggga
     1801 aaaacttatc agtaataaag tcaaaattgt tcgtttactt aataagagta agaaaagttt
     1861 actaaggggt atatttagtc gaacaaccgt tatcgctatt ttattgattt tacaattatt
     1921 gtttttacta gcctcttact catggctaga gcagtatcgt gtttggttag caaccgttga
     1981 gcatatctta acaattggag cggtcttata tttagttaat agtgaaatgg atgctttgtc
     2041 ccgagtaact tggttaatat tggtaatgat tgccccttta ctaggggcga tgttcctaat
     2101 gtacaccaaa ttcgactggg gttatcgtgg tttgaaacaa agattggaga cattgattga
     2161 tgagagtcag atttatctag aggatgatcc tgaaactttg aatcagctta agtcgagtac
     2221 atccacaaca tatcatttgg ttcaatattt tgaaaaagca catggaaatt tcccagttta
     2281 tcgaaatact gatgtgacat ttctaccaac gggagaagca ttctttgaaa aaatgaaaga
     2341 agaactacta aaagctaaga agtatatctt tttagaattc tttatcattg acgaaggaat
     2401 aatgtgggga gaaatactta gtattttaga acaaaaggtg gaagaaggtg ttgaagtccg
     2461 tatcttatat gatggtatga tagaaattac aaaattatcc tttgattata cgaaacgact
     2521 tgagaaaata ggaataaaag caaaagcatt ttctccgatt tctcctttta tttcaactta
     2581 ttataactat cgtgaccatc gaaaaattgt tgttattgat ggagtagtag gcatgactgg
     2641 tggtgtaaac ttagccgatg agtatattaa tcacattgag ttatttgggc attggaaaga
     2701 ttcaggtatt atgttaaagg ggaaagcagt ggatagtttc ctcctacttt tcttacagat
     2761 gtggtccatt actgaggaaa aaatgttagt agcgccttac ctcggtgtac acgatgattt
     2821 agttgaaaat gaaggctatg ttattcctta tggagattct cctttagaca ctgataaagt
     2881 aggggaaaat gtttatatag atattcttaa tcatgctaga gaatatgttt acatcatgac
     2941 accttattta atattagaca gtgaattgga acatgccatt cagtttgctg cggaacgtgg
     3001 tgtagatgta cgtattatta tgccgggtat cccagataag ccaatccctt atgctcttgc
     3061 taaaacttac tatcaagcat taataaaatc aggtgttaaa atatatgaat acacccctgg
     3121 atttgttcat tcaaaaatat tccttagtga taatacaaag gctgttgtag gtacgataaa
     3181 tcttgattac cgtagcctgt atcaccattt tgaatgtgct gtttatcttt ataaggtaga
     3241 tgcaatccaa gacatttatc gagattatat ggatacgtta aataaatcaa ggttagttag
     3301 cttgaaagat ataaataata ttccaaaatt ccaaaaagta attggcatag tgacaaaaac
//
LOCUS       DBJYSH010000146         3298 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.146, whole genome shotgun sequence.
ACCESSION   DBJYSH010000146 DBJYSH010000000
VERSION     DBJYSH010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 3298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3298
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.146"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            complement(<1..214)
                     /locus_tag="ACUBV3_001877"
     CDS             complement(<1..214)
                     /locus_tag="ACUBV3_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3848350.1"
                     /translation="MKDYINRILHFIKEHMTYHVNFIDDFLDIKWEKVSNIHLRFWTT
                     IIAYLVIFILSISTVILNLVLLFQGFL"
     gene            complement(364..1104)
                     /locus_tag="ACUBV3_001878"
     CDS             complement(364..1104)
                     /locus_tag="ACUBV3_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003107174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HHU3848351.1"
                     /translation="MAELKIDVQDLHKSYGQNEVLKGIDAKFYEGDVVCIIGPSGSGK
                     STFLRTLNLLESITSGKVVVDGFELSNPKTDIDKARENIGMVFQHFNLFPHMSVLENI
                     TFAPIELGKESKEAAEKHGMELLEKVGLADKANAKPDSLSGGQKQRVAIARSLAMNPD
                     ILLFDEPTSALDPEMVGDVLNVMKDLAEQGMTMLIVTHEMGFARQVANRVIFTDGGRF
                     LEDGTPEQIFDTPQHPRLQDFLNKVLNV"
     gene            complement(1104..3287)
                     /locus_tag="ACUBV3_001879"
     CDS             complement(1104..3287)
                     /locus_tag="ACUBV3_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HHU3848352.1"
                     /translation="MKHKLKAFTLALLSIFFVFGGKVSAETVNIVSDTAYAPFEFKDS
                     DQTYKGIDVDIVNEVAKRAGWNVNMTYPGFDAAVNAVQSGQADALMAGTTVTEARKKV
                     FNFSDTYYDTSVILYTKNNNKVTNYKQLKGKVVGVKNGTAAQSFLEENKSKYGYKVKT
                     FDTSDLMNNSLDSGSIYAAMDDQPVVQFAINQGKAYAINMEGEAVGSFAFAVKKGSGH
                     DNLIKEFNTAFAQMKSDGTYNDIMDKWLGKDATKTSGKATGNANEKATPVKPSYKIVS
                     DSSFAPFEYQNGKGKYTGFDMELITKIAKQQGFKLDISNPGFDAALNAVQSGQADGVI
                     AGATITEARQKIFDFSDPYYTSSVILAVKKGSNVKSYQDLKGKTVGAKNGTASYTWLS
                     DHADKYNYHVKAFDEASTMYDSMNSGSIDALMDDEAVLAYAINQGRKFETPIKGEKSG
                     DIGFAVKKGANPELIKMFNNGLASLKKSGEYDKLVKKYLSTASTSSNDKAAKPVDEST
                     ILGLISNNYKQLLSGIGTTLSLTLISFAIAMVIGIIFGMMSVSPSNTLRTISMIFVDI
                     VRGIPLMIVAAFIFWGIPNLIESITGHQSPINDFVAATIALSLNGGAYIAEIVRGGIE
                     AVPSGQMEASRSLGISYGKTMQKVILPQAVRLMLPNFINQFVISLKDTTIVSAIGLVE
                     LFQTGKIIIARNYQSFRMYAILAIMYLVIITLLTRLAKRLEKRLK"
BASE COUNT         1018 a          602 c          576 g         1102 t
ORIGIN      
        1 ttaagaatcc ttgaaaaaga agtacaaggt taagaattac ggtagagata cttaagataa
       61 aaataactag ataagcaata atagttgtcc aaaaccttaa atggatgtta ctcacctttt
      121 cccatttaat atctaaaaag tcatctataa agttgacatg ataagtcata tgttctttaa
      181 taaagtgcaa tattctgtta atatagtctt tcatactgct atattatcat actgaaagcc
      241 cttgtgcaac attattcaaa cattctagct gatgttttgc taattttaga agaaaactta
      301 tcctattata atttgtttgt gatcaggata taaaaaaaga tgacacgaag tcatcttttt
      361 ttattaaacg tttaaaactt tattaaggaa gtcttggaga cgtgggtgtt gaggtgtatc
      421 aaagatttgc tcaggtgtcc catcttcgag gaaacgacca ccatctgtaa agataacacg
      481 attggcaact tgacgagcaa atcccatttc gtgtgtcaca attaacatag tcataccttg
      541 ttcagctaaa tctttcataa cattgagaac atctccaacc atttctggat caagtgcaga
      601 agttggttcg tcaaaaagta agatatctgg attcatcgct aagcttcttg cgatagctac
      661 acgttgtttt tgtcctccag ataaactatc tggtttggca ttagctttat cggctaaacc
      721 aactttttca agtaattcca tcccatgctt ttcagctgct tctttagatt ctttaccaag
      781 ttctatagga gcaaaagtga tgttttcaag aactgacata tgtggaaaaa gattgaagtg
      841 ttgaaatacc attccgatat tttcacgtgc tttgtctatg tctgtttttg gatttgataa
      901 ctcaaagccg tccacaacaa cttttccact tgtgattgat tcaagtaagt tcaatgtgcg
      961 aaggaaagtt gattttcctg aaccagaagg tccaataata caaacaacat caccttcata
     1021 aaatttagca tcaatacctt ttaaaacttc attttgccca tacgacttgt gtaaatcttg
     1081 gacatcaatt tttaattctg ccattattta agcctctttt ctaaacgttt tgctagacgg
     1141 gtgagaaggg taatgattac aagatacata attgcaagga tagcatacat acggaatgat
     1201 tgatagttac gtgcaattat gattttacca gtttggaaga gttccacaag tccgattgct
     1261 gatacaattg ttgtatcctt taatgagatg acaaattggt tgataaagtt tggtaacata
     1321 aggcgtactg cttgaggtaa gataaccttt tgcatagttt tgccgtaaga aatacctaag
     1381 ctgcgacttg cttccatttg accagaagga acagcttcaa taccaccacg tacaatttca
     1441 gcaatgtacg caccaccatt taaagaaaga gcgatagtag cagcaacgaa gtcattaatt
     1501 ggactttggt gacctgtgat gctttcgatt aaattaggaa taccccagaa aataaaagcg
     1561 gccacaatca tgagtggaat accacggaca atatcaacaa aaatcattga aattgtgcgg
     1621 agagtattac ttggtgatac gctcatcata ccaaagataa taccaataac catagcaatc
     1681 gcaaacgaga taagagttaa acttaaagta gttccaatac cagatagcaa ttgtttgtag
     1741 ttattagaaa ttaaccctaa aatagttgat tcatctacag gtttagcagc tttatcgttt
     1801 gaagaagtgc tggctgtgga aaggtatttt ttaacaagtt tatcgtactc acccgatttt
     1861 ttgagtgaag caagaccgtt gttaaacatt ttaattaatt ctggatttgc cccttttttc
     1921 actgcaaatc cgatatcgcc tgatttttca cctttgatag gtgtttcaaa tttacgacct
     1981 tgattaatag cgtaagcaag aacggcttcg tcatccatta gagcatcaat tgaacctgag
     2041 ttcatactat catacattgt agatgcttca tcaaatgctt taacatgata gttgtactta
     2101 tctgcgtggt ctgataacca agtatatgag gcagtaccat ttttagcacc aactgttttt
     2161 ccttttaaat cttggtatga tttgacattg cttccttttt taaccgctaa gataacgcta
     2221 gatgtgtaat aaggatcaga aaaatcaaag attttttggc gtgcttctgt gattgtggct
     2281 cctgcaataa caccgtcagc ttgcccagat tggacagcat ttaaagcggc atcaaaacct
     2341 ggatttgaga tatcaagttt aaaaccttgc tgtttagcaa ttttcgtgat taattccata
     2401 tcaaaaccag tatatttccc tttaccgttt tgatattcga atggtgcgaa tgaagaatca
     2461 gaaacaattt tataacttgg ctttacagga gttgcttttt cattggcatt acctgttgct
     2521 ttgccgcttg tttttgtagc gtcttttcca agccatttat ccatgatgtc attataagtg
     2581 ccatctgatt tcatttgtgc aaaagctgtg ttaaattctt taattagatt atcgtgtcca
     2641 ctaccttttt tgacagcaaa tgcaaagcta ccaactgctt cgccttccat gttaatggcg
     2701 taagcttttc cttgatttat cgcaaattgc acaactggtt gatcgtccat agcggcgtaa
     2761 atagaaccag aatcaaggct gttattcatt aggtcgcttg tatcaaatgt tttaacttta
     2821 tagccgtatt tagatttatt ttcttctaag aagctttgag cagctgttcc attttttaca
     2881 ccgactactt ttccttttag ttgtttgtag tttgtgactt tattattatt tttagtataa
     2941 agaataacgg aagtatcgta ataagtatct gagaaattaa agactttttt acgtgcttca
     3001 gtaacagtag ttccggccat tagcgcatct gcctgtccag attgaacagc gttaactgcg
     3061 gcatcaaaac ctggatacgt catgttaaca ttccagccag cacgcttagc gacttcgtta
     3121 acgatgtcaa catcgattcc tttataagtt tgatcagaat ctttaaattc gaatggagcg
     3181 tatgctgtat cagaaacaat attcacagtc tctgcactga cctttccacc aaacacaaag
     3241 aatattgaga gtaaagcaag cgtaaaagct tttaacttgt gcttcatgta aatctcct
//
LOCUS       DBJYSH010000147         3203 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.147, whole genome shotgun sequence.
ACCESSION   DBJYSH010000147 DBJYSH010000000
VERSION     DBJYSH010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 3203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3203
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.147"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            192..1388
                     /gene="tuf"
                     /locus_tag="ACUBV3_001880"
     CDS             192..1388
                     /gene="tuf"
                     /locus_tag="ACUBV3_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HHU3848353.1"
                     /translation="MAKEKYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLARRLPTSV
                     NQPKDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQM
                     DGAILVVASTDGPMPQTREHILLSRQVGVKHLIVFMNKVDLVDDEELLELVEMEIRDL
                     LSEYDFPGDDLPVIQGSALKALEGDEKYEDIIMELMSTVDEYIPEPERDTDKPLLLPV
                     EDVFSITGRGTVASGRIDRGTVRVNDEVEIVGIKEDIQKAVVTGVEMFRKQLDEGLAG
                     DNVGVLLRGVQRDEIERGQVLAKPGSINPHTKFKGEVYILSKEEGGRHTPFFNNYRPQ
                     FYFRTTDVTGSIELPAGTEMVMPGDNVTIEVELIHPIAVEQGTTFSIREGGRTVGSGI
                     VSEIEA"
     gene            1569..2327
                     /gene="tpiA"
                     /locus_tag="ACUBV3_001881"
     CDS             1569..2327
                     /gene="tpiA"
                     /locus_tag="ACUBV3_001881"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HHU3848354.1"
                     /translation="MSRKPFIAGNWKMNKNPEEAKAFIEAVASKLPSSELVEAGIAAP
                     ALTLSTVLEAAKGSELKIAAQNSYFENSGAFTGENSPKVLAEMGTDYVVIGHSERRDY
                     FHETDQDINKKAKAIFANGLTPIICCGESLETYEAGKAVEFVGAQVSAALAGLSEEQV
                     SSLVIAYEPIWAIGTGKSATQDDAQNMCKAVRDVVAADFGQAVADKVRVQYGGSVKPE
                     NVAEYMACPDVDGALVGGASLEAESFLALLDFVK"
     gene            2504..3196
                     /locus_tag="ACUBV3_001882"
     CDS             2504..3196
                     /locus_tag="ACUBV3_001882"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018029419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HHU3848355.1"
                     /translation="MVKLVFARHGESEWNKANLFTGWADVDLSEKGTQQAIDAGKLIQ
                     AAGIEFDLAFTSVLKRAIKTTNLALEAADQLWVPVEKSWRLNERHYGGLTGKNKAEAA
                     EQFGDEQVHIWRRSYDVLPPDMAKDDEHSAHTDRRYASLDDSVIPDAENLKVTLERAL
                     PFWEDKIAPALKDGKNVFVGAHGNSIRALVKHIKQLSDDEIMDVEIPNFPPLVFEFDE
                     KLNLVSEYYLGK"
BASE COUNT         1032 a          606 c          640 g          925 t
ORIGIN      
        1 taaaaaatac atataagaga taacaaacgc ctcaatcaat cttacccgaa ttttaaggaa
       61 ataaagaaat aagcaaaaaa gcataaaata cttgcttttt tagaggattt tgttaaaata
      121 gataagtaaa gttagactgt attgcctacc gtctatctat aaaatatatt ttattggagg
      181 cttttcctaa aatggcaaaa gaaaaatacg atcgtagtaa accacacgtt aacattggta
      241 caatcggaca cgttgaccac ggtaaaacta ctttaacagc agctatcaca actgtattgg
      301 cacgtcgctt gcctacttca gttaaccaac caaaagatta cgcttctatc gatgctgctc
      361 cagaagagcg cgaacgcgga atcacaatca acactgcaca cgttgagtac gaaactgaaa
      421 aacgtcacta tgcccacatc gacgctccag gacacgcgga ctacgttaaa aacatgatta
      481 ctggtgctgc tcaaatggac ggagctatcc ttgtagttgc ttcaactgat ggaccaatgc
      541 cacaaactcg tgagcacatc cttctttcac gtcaagttgg tgttaaacac cttatcgtat
      601 tcatgaacaa agttgacctt gttgatgatg aagaattgct tgaattggtt gaaatggaaa
      661 ttcgtgacct tctttcagaa tacgacttcc caggtgatga ccttccagtt atccaaggtt
      721 cagctcttaa agcacttgaa ggcgatgaaa aatacgaaga catcatcatg gaattgatga
      781 gcactgttga tgagtacatt ccagaaccag aacgtgatac tgacaaacct ttacttcttc
      841 cagtcgaaga tgtattctca atcactggac gtggtacagt tgcttcagga cgtatcgacc
      901 gtggtactgt tcgtgtcaac gacgaagttg aaatcgttgg tattaaagaa gatatccaaa
      961 aagcagttgt tactggtgtt gaaatgttcc gtaaacaact tgacgaaggt cttgcagggg
     1021 acaacgttgg tgttcttctt cgtggtgttc aacgtgatga aatcgaacgt ggtcaagttc
     1081 ttgctaaacc aggttcaatc aacccacaca ctaaatttaa aggtgaagtt tacatccttt
     1141 ctaaagaaga aggtggacgt catactccat tcttcaacaa ctaccgtcca caattctact
     1201 tccgtacaac tgacgtaaca ggttcaatcg aacttccagc aggaacagaa atggttatgc
     1261 ctggtgataa cgttactatc gaagttgaat tgattcaccc aatcgccgta gaacaaggta
     1321 ctactttctc aatccgtgaa ggtggacgta ctgttggttc aggtatcgtt tcagaaatcg
     1381 aagcttaatt taacttaatt aagttcccaa acggcagtta gagtcagaca gctcaaattg
     1441 tgaagagtcc tcagggactc ttttttagta tctataatta aagcgtttta ctagactcgt
     1501 gaaactttta attaactata atttatggta tgatagatta gtaaataaaa aaagaagagg
     1561 tatgtgtaat gtcacgtaaa ccatttatcg ctggtaactg gaaaatgaat aaaaatccag
     1621 aagaagcaaa agcattcatc gaagcagtag cttctaaatt accatcatca gaacttgttg
     1681 aagcagggat tgctgctcca gcacttactt tatcaacagt tcttgaagca gctaaaggtt
     1741 cagaattgaa aatcgctgct caaaatagct attttgagaa ttcaggtgca ttcactggtg
     1801 aaaacagccc taaagtattg gctgaaatgg gtacagacta cgttgttatc ggtcactcag
     1861 aacgtcgtga ttatttccat gaaactgacc aagatatcaa caaaaaagca aaagctatct
     1921 ttgctaatgg tttgactcca atcatttgtt gcggtgagtc tcttgaaact tacgaagctg
     1981 gtaaagcggt tgaattcgta ggtgctcaag tttcagcagc tcttgcaggt ttgtcagaag
     2041 agcaagtatc atcacttgtt atcgcttacg aaccaatctg ggctattggt actggtaaat
     2101 cagcaactca agacgacgca caaaacatgt gtaaagcagt tcgtgacgtt gttgcagcag
     2161 acttcggtca agcagttgct gacaaagttc gcgttcaata cggtggttca gttaaacctg
     2221 aaaacgttgc agaatatatg gcttgcccag acgttgacgg tgcccttgta ggtggagctt
     2281 cacttgaagc agaaagcttc cttgccttat tagattttgt taaataaatc acatgatatc
     2341 taaaggcata gcactctagg tgatatgcct tttcttatag atagttaata ataagctatt
     2401 tttgattatt gaactagtaa tcagactaga tttttatggc tattttttgt agaatagtta
     2461 aagtaaatat tggtatagcc aaaattaaag gagaaatttt ctaatggtaa aattagtatt
     2521 cgcacgccac ggtgaatctg agtggaataa agctaacctt ttcactggat gggctgacgt
     2581 agatctttca gaaaaaggta cacaacaagc tattgatgct gggaaattaa ttcaagcagc
     2641 aggtattgag ttcgaccttg cttttacatc agttcttaaa cgtgccatca aaacaactaa
     2701 ccttgccctt gaagcagctg atcaactttg ggtaccagtt gaaaaatcat ggcgcttgaa
     2761 cgaacgtcat tacggtggat tgacaggaaa aaataaagca gaagcagctg aacaatttgg
     2821 tgatgagcaa gttcatattt ggcgtcgttc atatgatgta ttgcctccag atatggctaa
     2881 agatgatgaa cattcagcac atactgatcg tcgctatgct tcactagatg attctgttat
     2941 tccagatgca gaaaacctaa aagttacttt agagcgtgct cttcctttct gggaagataa
     3001 aattgctcct gctcttaaag atggtaaaaa tgtgtttgtt ggtgcacacg gtaactcaat
     3061 ccgtgctctt gtaaaacata tcaaacaatt gtcagatgat gaaatcatgg acgttgaaat
     3121 tcctaacttc ccaccacttg ttttcgaatt tgatgaaaaa ttaaaccttg tttcagaata
     3181 ttacttaggt aaataatcta atc
//
LOCUS       DBJYSH010000148         3192 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.148, whole genome shotgun sequence.
ACCESSION   DBJYSH010000148 DBJYSH010000000
VERSION     DBJYSH010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 3192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3192
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.148"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            complement(743..3151)
                     /locus_tag="ACUBV3_001883"
     CDS             complement(743..3151)
                     /locus_tag="ACUBV3_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK signal domain/LPXTG anchor domain surface
                     protein"
                     /protein_id="HHU3848356.1"
                     /translation="MFRKYNFEKGLKFSIRKFSVGVASVAIGSLLFISPQVLADETTS
                     VTSATTPTGVTATDANLVTSNNSTPTSTNRSATSTQGSNLSNTSEIIKPATLAATSPT
                     TDNVAPSVDKRTYATSGDWTLQNPYADSVRNKNISPSVRDESFKSAETTVVRDDNSTV
                     KVTATITPVPGNDEGSGVLTNGGNQSEYKATSEMFVGNVDPAKIPALGVYTQPGRTEG
                     GSKLSDKLNFNGKAPSTILTLKFDKAVTDPIIDLSGVGGNAHLSFTETTNENGRIVEK
                     IDYARGSYNSTFFELITPGISLEKASSGVNLTVTANTVDVTDKNTFNESVVNPSDADF
                     VNGLDRTPDAVPAGTGSIRLKGTFTEVSFKLYHQAVPFTAFSKEKYHTGDGYYNSTAN
                     IRPTVVKPDSINGLNKHASDVIDDKISDNNDISNDDLLRLSVRLQNPRGSVVVNYIDT
                     EGNIIGTEYKDTTDAILGTHYNTAESSGDLSSDATVERPFTITKDGKVYELVAENITV
                     PVGKVNSDGTLATNGGSFNYGTDAASGEVAEGTKSVTYVYSIKQESKGNVHARYVILG
                     TETELASVKTVKSEAPIDEAYSDRAPATLEKDGKLYEFVHVRDNKGDAPADGKVTEQD
                     QTITYEYKLKKDESEAVGNVVINYVDETGNVIKKPILDTHESKVGTPYDTTDYKFAEI
                     KFNGKIYKLVSAKTMGNEFGKVTEGTTEVTYVYRESVKSTLPKEMGISIPSESESPKF
                     ISTQTTENRPNKGVLTSSKKPTNKSVLPTTGEESNRTLGVVGITLVATTATLAASSLK
                     RRKN"
BASE COUNT          952 a          608 c          500 g         1132 t
ORIGIN      
        1 atataaaaat ttcaaattga gactgctcaa taaatatggt atcggcatca ttgataaact
       61 ttctttttaa tgtcttataa taaaattcca taaaatatat cctatgaatt tccttttagt
      121 ttcacactag caaggggttt ctctcctaaa tatatctcgg aatcatattt cagtatatta
      181 agagtcttta acggtatagg ctattaacct ttctttgaaa ttttcttgat gataagtcta
      241 attgaaatta tccattacca acttgtcctg tattcgattt ccatccccta gccaacaatc
      301 ttttagttat aaacttctaa taacacctaa taaataaaga gtacttactt ttatcgaaat
      361 atagaacaag actctctttg ctaggtttta tttttatata ttttgaagat agtctgtact
      421 ttatataggt ataaaaaccg ggataggaaa tttggtgcat ttaacatagg tatattcagt
      481 ttagcattat cttcctttta attagagcgt taagccctct aataatattt tattcccttt
      541 ttaaaatgat aaattgcaaa aaaagccatc aaatgatgac tttttatgta gactcgcaag
      601 acgctcctct ttagatatag ctataattaa ctcattacaa tccaaacaca ccataacaaa
      661 tagttcatca aaatctatga tgtgattatt aacaaaagaa tcttataata acggtattgg
      721 atcacatcga gtcagaagaa ttttaatttt tacgtcgttt taaactgcta gctgccaaag
      781 tagctgttgt tgctactaga gtaatcccaa caactccgag agttctatta ctttcctcgc
      841 ctgtagttgg aagaacagat ttattggttg gttttttaga tgaagttaat actcctttgt
      901 taggtctatt ttctgtagtt tgggtagata taaacttagg agactctgat tcgcttggaa
      961 tagaaattcc catttccttt ggtagggtag attttactga ttctcgatac acataagtca
     1021 cttctgttgt gccttcagtg acctttccga attcatttcc catagttttt gcagaaacga
     1081 gtttataaat cttaccatta aatttaattt cagcgaattt ataatctgtg gtatcatatg
     1141 gtgtcccaac ctttgattcg tgtgtatcta gtattggttt tttgataaca ttacctgttt
     1201 cgtccacata gttaatcact acattcccta cagcctctga ttcatctttc ttaagcttat
     1261 attcatatgt aatggtctga tcttgttcag tcaccttacc atctgctgga gcatcgccct
     1321 tattatcacg cacatgtaca aattcataca acttaccatc tttttcaaga gttgcaggtg
     1381 ctctatctga atatgcttca tcaattggag cttctgattt aactgtttta acacttgcaa
     1441 gctcagtctc tgtccctaaa ataacatagc gggcatgaac attgcccttt gattcttgtt
     1501 taatgctata aacataagtt actgacttag taccttctgc aacttctcca gatgctgcat
     1561 cagtaccata attaaatgag cctccatttg tagccaacgt accatccgaa ttaaccttac
     1621 caactggtac tgtaatattt tctgctacta attcatacac cttaccatct ttagtaatag
     1681 tgaaaggacg ctcaactgtc gcgtctgaac taaggtctcc tgatgattct gccgtattgt
     1741 aatgtgttcc tagaattgca tcggtggtat ctttgtattc agtaccaata atattgcctt
     1801 ctgtatcaat ataatttaca acaactgatc cacgcggatt ttgtaagcga acagataaac
     1861 gaagtaagtc atcgttcgaa atatcattat tatcggaaat tttatcatca ataacatcgc
     1921 ttgcgtgctt atttaaacca ttaatactat ctggtttaac tacagttggt cgaatattcg
     1981 cagtactatt ataatatcca tctcctgtat gatatttctc tttagaaaat gctgtaaatg
     2041 gaacagcttg atgatataat ttaaatgaaa cctctgtaaa ggttcctttt aaacgaattg
     2101 aacctgttcc cgctgggact gcatcaggcg ttctatctag accattgaca aagtctgcgt
     2161 cagatggatt aacaactgac tcattaaagg tgtttttgtc tgttacatca accgtattcg
     2221 ctgtaactgt taaattaact cctgaacttg ctttttcaag gcttatacct ggagttatta
     2281 actcaaagaa cgttgaatta tatgaaccac gtgcatagtc aattttttca acaatcctac
     2341 cattttcatt ggtagtttct gtaaatgata gatgtgcatt acctccaaca ccagataagt
     2401 caataatagg gtctgtaact gccttgtcaa atttcaatgt taaaatagtt gatggcgcct
     2461 tcccattaaa attgagctta tcagatagtt tactacctcc ttcagtacgt cctggttgtg
     2521 tatagacacc taaagcaggt atttttgcag gatctacatt tccaacaaac atttctgatg
     2581 ttgctttata ttctgactga ttaccaccat ttgttaaaac accagaacct tcatcattcc
     2641 ctggtacagg agtaattgtc gcagtcactt tgacggttga gttatcgtca cgaaccaccg
     2701 ttgtttcagc acttttaaat gattcatcac gaacacttgg agaaatattt ttatttcgaa
     2761 cactgtcagc atatggattt tgtaacgtcc aatcgccact agtagcatac gtcctcttgt
     2821 ctactgatgg ggctacatta tcagttgttg gtgatgttgc tgctaaagtt gcaggcttaa
     2881 tgatttctga agtattactc aaattacttc cttgagtgct agttgcactc ctattagtgg
     2941 aagtaggagt tgaattgtta gaagtgacca agtttgcatc tgtggccgtt acacctgttg
     3001 gagttgttgc agatgtaaca ctagttgtct catctgcaag tacctgagga gaaatgaata
     3061 ataatgatcc tatagcaact gaagctactc caaccgaaaa tttacgaatg gaaaacttta
     3121 aacctttttc aaaattatat ttacgaaaca taatatcaac ctccctgtaa cagtttttat
     3181 atttcacata aa
//
LOCUS       DBJYSH010000149         3165 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.149, whole genome shotgun sequence.
ACCESSION   DBJYSH010000149 DBJYSH010000000
VERSION     DBJYSH010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 3165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3165
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.149"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            76..615
                     /locus_tag="ACUBV3_001884"
     CDS             76..615
                     /locus_tag="ACUBV3_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HHU3848357.1"
                     /translation="MSITSVKKSKPFKLGVAGLLVGASLALPLSVSAASYTVKSGDTL
                     SAIAKNHKTTVQELVSLNSISNADVISIGDVLKLDNSTASQAEAKSQPTIENSMNSSS
                     NLSSSDSAAKEEIARRESNGSYTAQNGQYYGRYQLSQSYLNGDLSPENQEKVADNYVV
                     SRYGSWSAALSFWNSNGWY"
     gene            721..1425
                     /locus_tag="ACUBV3_001885"
     CDS             721..1425
                     /locus_tag="ACUBV3_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HHU3848358.1"
                     /translation="MNKRRKLSKLNVKKHHLAYGAITLVALFSCILAVMVIFKSSQVT
                     TESLSKADKVRVAKKSKMTKATSKSKVEDVKQAPKPSQASNEAPKSSSQSTEANSQQQ
                     VTASEEAAVEQAVVTENTPATSQAQQAYAVTETTYRPAQHQTSGQVLSNGNTAGAIGS
                     AAAAQMAAATGVPQSTWEHIIARESNGNPNVANASGASGLFQTMPGWGSTATVQDQVN
                     SAIKAYRAQGLSAWGY"
     gene            complement(1560..2432)
                     /gene="sdaAA"
                     /locus_tag="ACUBV3_001886"
     CDS             complement(1560..2432)
                     /gene="sdaAA"
                     /locus_tag="ACUBV3_001886"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HHU3848359.1"
                     /translation="MFYTIEELVEQANSQHKGNIAELMIQTEIEMTGRSREEIRYIMS
                     RNLEVMKASVIDGLTPSKSISGLTGGDAVKMDQYLQSGKTISDTTILAAVRNAMAVNE
                     LNAKMGLVCATPTAGSAGCLPAVISTAIEKLNLTEEEQLDFLFTAGAFGLVIGNNASI
                     SGAEGGCQAEVGSASAMAAAALVMAAGGTPFQASQAIAFVIKNMLGLICDPVAGLVEV
                     PCVKRNALGSSFALVAADMALAGIESQIPVDEVIDAMYQVGSSLPTAFRETAEGGLAA
                     TPTGRRYSKEIFGE"
     gene            complement(2447..3115)
                     /gene="sdaAB"
                     /locus_tag="ACUBV3_001887"
     CDS             complement(2447..3115)
                     /gene="sdaAB"
                     /locus_tag="ACUBV3_001887"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HHU3848360.1"
                     /translation="MKHLKFQSVFDIIGPVMIGPSSSHTAGAVRIGKVVHSIFGEPSE
                     VTFHLYNSFAKTYQGHGTDKALVAGILGMDTDNPDIKNSLEIAHQKGIKIYWDILKDS
                     NSPHPNTAKITVKNGDRSMSITGVSIGGGNIQVTELNGFSVSLTMNTPTLIIVHQDIP
                     GMIAKVTDILSDFNINIAQMNVTRESAGEKAIMIIEVDSRDCQQAVKKIEAIPHLHNV
                     NFFD"
BASE COUNT         1040 a          618 c          553 g          954 t
ORIGIN      
        1 tctaaggcaa aacaattagt aatatttaca aggtatgata gtacgcgtat caattaatga
       61 aagagagcat atattatgtc aataacctcg gttaaaaaat caaaaccatt taaattagga
      121 gtggcaggtc ttttagtggg tgcttcatta gctttaccac tttcagtaag cgcagcatct
      181 tataccgtga aatcaggtga taccttatca gctattgcta aaaatcataa aactacggta
      241 caagagttag tgtctctcaa tagtatcagt aacgctgatg tcatcagtat aggtgatgtt
      301 ttaaaattgg ataattctac agctagtcaa gcagaagcaa aatctcaacc aacaattgaa
      361 aattcaatga attcttcatc aaatttgagt tcaagtgatt cagccgcaaa agaagaaata
      421 gctcgtcgtg aatcaaatgg tagttatact gcacagaatg gacaatatta tggaagatat
      481 caactgtctc aatcttacct aaatggcgac ttatctcctg aaaatcaaga aaaagtagcg
      541 gacaattatg tggtttctcg ttacggatct tggtcggcag cgctatcatt ttggaatagt
      601 aacggctggt attaataaaa gttgataaaa caaagagatt tacttaaaat taaatctctt
      661 tgtaatattt ttaacatcta ttcaattgca aggtaacaaa taaagtataa tataggtaac
      721 atgaataaaa gaagaaaatt atcaaaattg aatgtaaaaa aacatcattt agcttatgga
      781 gctatcactt tagtagccct tttttcatgt attttggctg taatggtcat ctttaaaagt
      841 tcacaagtta ctactgaatc tttgtcaaaa gcagataaag ttcgcgtagc caaaaaatca
      901 aaaatgacta aggcgacatc taaatcaaaa gtagaagatg taaaacaggc tccaaaacct
      961 tctcaggcat ctaatgaagc cccaaaatca agttctcaat ctacagaagc taattctcag
     1021 caacaagtta ctgcgagtga agaggcagct gtagaacaag cagttgtaac agaaaacacc
     1081 cctgctacca gtcaggcaca acaagcttat gctgttactg agacaactta tagacctgct
     1141 caacaccaga cgagtggcca agtattgagt aatggaaata ctgcaggggc tattggctca
     1201 gcagctgcag cacaaatggc tgctgcaaca ggagtccctc agtctacttg ggaacatatt
     1261 attgcccgtg aatcaaatgg taatcctaat gttgctaatg cctcaggagc ttcaggactt
     1321 ttccaaacga tgccaggttg gggttcaaca gctacagttc aggatcaagt taattcagct
     1381 attaaagctt atcgtgctca aggtttatca gcttggggtt actagtgaat agctataaat
     1441 taactttaca ctgttgtcaa caataattac agattgctaa aacaaataaa taaaaacgct
     1501 acagtaaagc tgtagcgttt ttatttattt tcagggaaag agtagaaaat tgataaactt
     1561 tattccccaa aaatttcttt actataacgt cttcctgtcg gcgtggcagc aagtcctcct
     1621 tctgcagtct cacgaaaagc agtcggtaaa cttgatccaa cttgatacat tgcatcaata
     1681 acttcatcta ctggaatttg cgattcaata ccagccaagg ccatatcagc agcaacaagt
     1741 gcaaaacttg atccaagagc attccgcttc acacaaggga cttcaactaa acctgcaaca
     1801 gggtcacaga taagtccaag catattttta ataacaaatg ctatagcttg gctagcttgg
     1861 aaaggagtac ctccagcagc cataactaaa gcagccgcag ccatagcact agctgaccca
     1921 acttcagctt ggcaacctcc ttctgcacct gagatagagg cattattacc aatgacgaga
     1981 ccaaatgcgc cggctgtaaa tagaaaatca agttgctctt cttctgttaa attaagcttt
     2041 tcaatggctg tagaaatcac agctggtaaa catcctgcac tacctgcagt tggtgttgca
     2101 cagaccagtc ccatcttagc atttaactca ttaacagcca tagcattcct aacggcagct
     2161 aggattgtgg tatctgaaat agtttttcct gattgtaaat attgatccat cttgacagca
     2221 tcaccgcctg ttaaaccact gattgattta ctaggggtta atccatcaat aacagaagct
     2281 ttcatgactt caagatttcg ggacataata taacgaattt cttcacgact tctaccagtc
     2341 atttcaattt ccgtttggat catgagctct gctatgttac ccttatgttg gctattagct
     2401 tgctctacca gctcttcaat tgtataaaac atagttaccc tttctcttaa tcaaagaagt
     2461 tgacattgtg taaatgagga atagcttcta tctttttgac agcttgttga caatctcgtg
     2521 aatctacttc tataatcatg atagcttttt ctcctgcact ttcgcgtgtt acattcattt
     2581 gagcaatatt aatgttaaag tcagataaaa tatcagttac tttagcaatc atgccaggta
     2641 tatcttgatg aacgataatt aaagtaggag tattcattgt taatgaaact gagaatccat
     2701 tcaactcagt aacttggatg tttccgccac caattgacac tcctgtaata ctcatgctcc
     2761 tatccccatt tttaacagtt attttcgcag tatttggatg tggtgaatta ctatctttta
     2821 ggatatccca gtaaatctta attccttttt gatgagcaat ttcaagagag tttttaatat
     2881 ctggattatc tgtatccatt cctagaatcc ctgcaaccaa tgctttatca gtaccgtgtc
     2941 cttggtaagt tttagcaaaa gaattgtata aatgaaaggt tacttcacta ggttcaccaa
     3001 aaatagaatg gacaacttta ccaatgcgga cagctcctgc agtatgacta cttgatggtc
     3061 caatcataac aggaccaata atgtcgaaga ccgattgaaa ttttaaatgt ttcatagttt
     3121 ttcctcgtga tttttgtaca ttctcattat aacagattat ttcta
//
LOCUS       DBJYSH010000150         3153 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.150, whole genome shotgun sequence.
ACCESSION   DBJYSH010000150 DBJYSH010000000
VERSION     DBJYSH010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 3153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3153
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.150"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            <1..1190
                     /locus_tag="ACUBV3_001888"
     CDS             <1..1190
                     /locus_tag="ACUBV3_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HHU3848361.1"
                     /translation="QKTVVLAGDYSYIRQIETTLKSLCVYHENLSIFIFNQDIPQEWF
                     LAMKDRVGQTGNQIQDVKLFHDHLSPKWENKKLNHINYMTYARYFIPQYISADTVLYL
                     DSDLVVTTNLDNLFQISLDNAYLAAVPALFGLGYGFNAGVMVINNQRWRQENMTIKLI
                     EKNQKEIENANEGDQTILNRMFENQVIYLDDTYNFQIGFDMGAAIDGHKFIFDIPITP
                     LPKIIHYISGIKPWQTLSNMRLREVWWHYNLLEWSSIISSKKVFGLDHPIKTQNYRLN
                     FLIVTTSDCIPSISELVTALPDCLFHIAAPTVMSDILISLLAFENIRLHKTYTPPIID
                     KLLAEADIYLDINVFGKMEGILQKMSQSEKPILTTRECNSQLVPETVFASIDDLINYL
                     QSI"
     gene            1255..2460
                     /locus_tag="ACUBV3_001889"
     CDS             1255..2460
                     /locus_tag="ACUBV3_001889"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000409543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HHU3848362.1"
                     /translation="MEKAVALAVDSNYLDKALVTIKSICVYNRNITFYLFNQDTPVEW
                     VRNINRKLEPLGSKLINVKIYNYDIAHLTTFLTVSTWFRLFLADYIPSSRVLYLDSDI
                     IVNTNLDYLFELDFKGYYLAAVKDPHKNEEGGFNAGMLLANLELWREDGLTKTLLKTA
                     EELHRVVKTGDQSILNIVCHNRWLSLNKTWNFQTYDVVSRYNHRSYLYLNIENRTPNI
                     IHFLTSDKPWNENSVARFRELWWYYFQLDFCQLTGKQRKVISYEKSMELLSVSDIHLF
                     TLTSSDNLEHIESLICRCPTVQFHIGAYTTVSNKLSKLEQYPNVLVYPELIEARIEKL
                     ITLATAYLDINHGPEVGNILQRVHLKQKPIYSFNNTSHQENITKHIVNHNSIDNMVVL
                     INELNLNQL"
     gene            complement(2507..3046)
                     /gene="nusG"
                     /locus_tag="ACUBV3_001890"
     CDS             complement(2507..3046)
                     /gene="nusG"
                     /locus_tag="ACUBV3_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HHU3848363.1"
                     /translation="MLDSFDKGWFVLQTYSGYENKVKENLLQRAQTYNMLDNILRVEI
                     PTQTVNVEKNGKTKEIEENRFPGYVLVEMVMTDEAWFVVRNTPNVTGFVGSHGNRSKP
                     TPLLEEEIRSILISMGQTVDVFDTNIKEGDVVQIIDGAFIGQEGRVVEIENNKVKLMI
                     NMFGSETQAELELYQVAEL"
BASE COUNT         1075 a          557 c          464 g         1057 t
ORIGIN      
        1 atcaaaaaac agttgttttg gctggtgatt attcctacat tagacaaatt gaaaccacat
       61 taaaatctct ctgtgtctat catgagaatc tctcaatttt tatttttaat caagatattc
      121 ctcaagaatg gtttttagct atgaaagata gggttggaca aactggaaat caaattcagg
      181 atgtaaagct cttccatgat cacttatccc caaaatggga aaataaaaag cttaatcata
      241 ttaattatat gacctatgct cgttatttca tacctcagta catctcagct gatacagttt
      301 tatatcttga ctctgactta gttgttacta ctaatttaga taacctcttt caaatttcac
      361 tagacaatgc atatttagct gcagttccag ctctttttgg gcttggatat gggtttaatg
      421 ctggagtaat ggtaattaac aaccaacgtt ggcgacaaga aaatatgact attaaattaa
      481 ttgaaaaaaa tcaaaaggaa attgagaatg ccaacgaagg ggatcaaaca attcttaatc
      541 gcatgtttga aaatcaggta atttatttag atgataccta caattttcaa attggttttg
      601 atatgggagc tgctatcgat gggcataaat ttatttttga catcccaatt accccactcc
      661 caaaaattat tcactacatt tcgggaatca aaccttggca aacattatca aatatgagac
      721 tccgtgaggt atggtggcac tataatttac ttgaatggtc aagtatcata tctagtaaaa
      781 aagtatttgg tttagaccac ccaattaaaa cacaaaatta tcgtctcaat ttccttattg
      841 ttacaacttc tgattgtata ccatctatct cagaattagt cactgccctt ccagattgtc
      901 tatttcacat tgcagcacca acagttatgt ctgacattct tataagccta ttagcttttg
      961 aaaatatccg ccttcataaa acttacactc cacctattat tgataaatta ttagcagaag
     1021 ctgatattta tcttgatatt aatgtttttg gaaaaatgga gggcattcta cagaaaatgt
     1081 cacaatctga aaaaccaatt cttactacaa gagaatgcaa tagccagctt gttccagaaa
     1141 cagtttttgc atctatcgat gatttaatta attatcttca gtctatataa aaaaattggc
     1201 atccattcac tatataccca acattattct aaacacttaa ctaggagaat ttaaatggaa
     1261 aaagcagttg cacttgcagt tgattcaaac tacttggata aagccttagt aacaataaag
     1321 tctatttgtg tttataatag aaatataact ttttatttat tcaatcaaga taccccagtt
     1381 gaatgggtac gtaatataaa caggaaacta gagcctctag gatcaaaact gattaatgtt
     1441 aaaatatata actatgatat tgctcatcta acgacttttc taactgttag tacatggttt
     1501 agattatttt tagcagatta tatacctagt tcacgtgtac tttatttaga ttcagatatt
     1561 atcgttaaca ctaatcttga ttacttattt gaactagatt ttaaaggtta ttacttagca
     1621 gccgtcaaag atccccataa aaatgaagaa ggagggttta atgctggcat gcttttagct
     1681 aatctagaac tatggcggga agatgggctc actaaaacat tactaaaaac agctgaagaa
     1741 ctccaccgag ttgtcaaaac aggggatcaa agtatcttga acattgtttg ccataatcgt
     1801 tggttatctc tgaacaaaac atggaacttt caaacttatg atgtcgttag ccgctataat
     1861 catcgatctt atttatatct aaacatagaa aatagaactc ctaatattat acatttttta
     1921 actagtgaca aaccttggaa tgaaaatagc gttgcaaggt ttagagaact atggtggtat
     1981 tacttccaac ttgatttttg ccaattaacc ggcaagcaaa gaaaagtgat ttcttacgaa
     2041 aagtccatgg aattgctttc tgtttcagat attcatcttt tcactcttac atcttccgat
     2101 aatttagaac acattgaatc gctaatttgt agatgtccta ctgttcaatt ccatattggt
     2161 gcctacacaa cagtgtcaaa taaacttagc aaactagaac aatatccaaa tgtcctagtt
     2221 taccctgaat taattgaggc aagaattgaa aaattaataa cattagccac tgcatacctt
     2281 gatattaatc atggtccaga agtaggtaat attttacaaa gagttcattt gaaacaaaag
     2341 ccaatttatt ctttcaacaa cacgtctcat caagagaata taaccaaaca tatcgttaat
     2401 cataattcta ttgataatat ggttgtttta attaacgaat taaacctaaa tcaattataa
     2461 attaacaaaa atccatcttt attttaagat ggatttttca gaagttttat aattctgcaa
     2521 cttggtacaa ctcaagttct gcttgtgttt ctgagccgaa catgttgatc attaatttaa
     2581 ctttgttatt ctcaatctca actacacgtc cttcttgtcc gatgaaagca ccatcaatga
     2641 tttgaacaac atcaccttcc ttgatattag tatcaaagac atcaacggtt tgtcccattg
     2701 agatgagtat tgaacgaatc tcttcttcta acaacggtgt tggtttagag cgattgccgt
     2761 gtgatccaac aaatccagta acgtttggag tattacgaac aacaaaccag gcttcatcag
     2821 tcattaccat ttccacaagt acataacccg ggaagcgatt ttcttcaatt tcttttgttt
     2881 tcccattttt ctcaacattt acagtttgag tagggatttc cacacgcaaa atgttgtcta
     2941 acatattata agtttgcgca cgttgcaaga gattttcctt tactttattt tcataacctg
     3001 agtaggtttg gagtacaaac caacccttat caaaagaatc taacataagt tttcctttcc
     3061 ttaataaaaa agccttttac ggcttattgg actgctattt tcaatatata ttataaagaa
     3121 aataaaataa gctgtcaatt aaagtaccga cta
//
LOCUS       DBJYSH010000151         3123 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.151, whole genome shotgun sequence.
ACCESSION   DBJYSH010000151 DBJYSH010000000
VERSION     DBJYSH010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 3123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3123
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.151"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            complement(1..2230)
                     /locus_tag="ACUBV3_001891"
                     /pseudo
     CDS             complement(1..2230)
                     /locus_tag="ACUBV3_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815001.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(2246..2509)
                     /locus_tag="ACUBV3_001892"
     CDS             complement(2246..2509)
                     /locus_tag="ACUBV3_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000538392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3848364.1"
                     /translation="MGVKSNEELKREQYLAEFSRVVTNFKWNASRIYFGDIIAIKLCF
                     KEALKQIDILHERAILRRDEVSTFYYWAKRNINNVYQELIEKK"
     gene            complement(2529..2840)
                     /locus_tag="ACUBV3_001893"
     CDS             complement(2529..2840)
                     /locus_tag="ACUBV3_001893"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3848365.1"
                     /translation="MTAKEAKNVINKNYNIVFYKKSCPYCKIAKKEILEKSKGSDITT
                     FFADVETVSGKELVRKYRISKAPSIVAVRGDNLKVNLYAKDDEDGRMIVEKKVIKDSF
                     R"
BASE COUNT          867 a          597 c          438 g         1221 t
ORIGIN      
        1 ctaaaaaccc acttaaagat aacagcatac ctaaaactgc aattaaacct gtcttagcat
       61 caccagttga tggcaagaca ctagatttta caactgatac ttgttcaaca tcaactgtct
      121 tagttacaac gtctttctta tcgttagcaa caactatttg ttgtgcttta acagcttctt
      181 gcagttttgg tttagaagta atataactag taattttacc tgtttcatca actactgcta
      241 ctgcttcttg tcttactttc tcaatttgag ctttttcttc tgctaatttc agtttttctt
      301 tagcttctaa aactgactta taagctgata aaactttttg atattgtgat tgcgcatcta
      361 ctgctttagt ttttaactct tgtaaataag ctacttctct ttgtaactta gcaatcttat
      421 ttgctaagtt atctttagct tgtgctacat cagactgtac tttaagcaaa ttagcttgtg
      481 cattgcgtag atttgtaaga taagcagttt tttgtgttag attatcttta gcttgcttgt
      541 aatcttgatt tgcttgtgct acttgttgac ttgcttctgc taacacttgt tttaattgat
      601 taagtttagt tgtatttgtt gtcaatttaa cctgtgcatc tgctaattgc ttttgagcat
      661 ctgttaattt agactctgct tttattaagt cagcttgttt tttaacatta tcttgattag
      721 ctaatttaac tgcttcttgt gctttagtga gtgcatcttg agcatctttt aaattagact
      781 atgctttaat caaattagct tgtgcaatcg gtgtctgcat agttactgca ttaagaccta
      841 ttgctttagc ttgtgcatca tttaattgtc tttgagcaac ttgttgactt gctaaagctt
      901 gtgactgctt agttaaggca ttagccatat ctttagtcat aattgagaaa aacggtacac
      961 ctacaccagc agtatcaata ctatctcgtg actgttcatt ccaaatgcgg tcaacactgt
     1021 aaaaaccatt gttatcacgg ttatacggat cataaacata cgtattgcca tttgaactac
     1081 ctctcaatac aatttcgtga ctgtattgag gtccccaagg actgaattta ttattttgta
     1141 cagcagcaac aacatgatga cctgctttca aagcttcttt tatagcattt tgacttgcta
     1201 aatgagtcac aacatagcca aacgctttag tagcttcaac aatacctctt ccacttgttc
     1261 caccaaaatt cttattaaat tcatttgttt ggttaaaaag ataatccgca acttcctttg
     1321 gtgtaactgt cttgcgtgat aaatcagaat aaaccatcgc taaagccgaa ggtacacatc
     1381 cagttgaagc aaaagtatgc cctccatata cgctagccca tgcgccgtca cgttgattgt
     1441 aataatgact ttgccctaga gcagttttaa acttattctg gttaacagta gcattagctt
     1501 gtgctaactg tgcatcttta acagataaat tttgttttgc gttatcaaga ttttgttgtg
     1561 cttttgcttt ttgattagta agtgttttat cagtcgcttg tgcttttgta agtgcatctg
     1621 tcgcagtttt aacatcagct gtcttagttg tgatgtttga ctgtgcttta tttaaatcac
     1681 tattaactgt agtattagtc agagcatctt tagctctatc cactgatgat tgagcttgtt
     1741 taacatcagt tgtcttagtt gcaacgtctt gcttatcgtg tgcaacaact atttgttgtg
     1801 ctttaacagc ttcttgcgct ttgacttgtt cagtttcagc agttttttgt ttagctccaa
     1861 tctgtgacaa catttgttta gctatatcaa ttgtttgttg tgctttaact ttattttctt
     1921 gagttgactc ttgagctagt ttttgtgttg cattcaactt tttagttgca ttgactagag
     1981 tttgttgagc ttgattttgt tgattttgtg cagtattaac tacattttgt tgtgtttcaa
     2041 catcactcgt tgctctatca gactttgctt ttgcactgtc aacttgttct tgtgttactt
     2101 ctgtaatgtg attagtttga ttcgcagttg tttctaacga taatgattta acttcgtcag
     2161 catttactgc tacgcttact gtacttaatg ttgcaattgc aatacctgat aaaactttag
     2221 tttgtttcat aaaaatctcc tcaatttact tcttttcaat caattcttga taaacattat
     2281 ttatatttct tttagcccag tagtaaaatg tactaacttc atctctacgt aaaatagctc
     2341 tttcatgtaa tatatctatt tgttttaaag cttctttaaa acaaagtttt attgcaatta
     2401 tatctccgaa atatattcta ctagcattcc acttaaaatt agtaaccacc ctactaaact
     2461 ctgctaaata ctgctctctt ttcaattcct cattactttt tacccccatg ataaatacct
     2521 ttctataact atctaaaact atcttttatt actttctttt ctacaatcat acgaccatcc
     2581 tcatcatctt ttgcatacaa attaactttc aagttgtctc ctcttacagc aactatgctt
     2641 ggcgctttgg atattctata tttacgtact agctccttgc cactaacagt ctccacgtca
     2701 gcaaaaaaag tagttatatc actaccttta cttttctcta aaatttcttt tttcgctatt
     2761 ttgcagtaag ggcaactctt tttataaaaa acaatattat agtttttatt aatcacattt
     2821 ttagcttctt tagcagtcaa ttgagaccta tagtactgat cactaaaaaa tgagatgcca
     2881 tcactcttta ataaaaaaac aagtgataca attaacgtca ctagtagcat cactaaagct
     2941 aaaaagacat ccttcctaac acaaacatac ctattcaatc tatatcccat acttcaaccg
     3001 acttccattt ttttctatta tctaaaaggt aagcaaccat acctgtttta caaataccct
     3061 tataaattaa aaaatcaccg tcagccacat acgatttaac aatagaataa tcgtaaaaag
     3121 ata
//
LOCUS       DBJYSH010000152         3040 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.152, whole genome shotgun sequence.
ACCESSION   DBJYSH010000152 DBJYSH010000000
VERSION     DBJYSH010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 3040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3040
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.152"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            complement(301..1086)
                     /locus_tag="ACUBV3_001894"
     CDS             complement(301..1086)
                     /locus_tag="ACUBV3_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HHU3848366.1"
                     /translation="MTKYQRMHFIFIKQYMKQIMEYKIDFFVGVLGVLLTQGLNLLFL
                     NVLFQHIPSLEGWTFQQIAFIYGFSLLPKGIDHLFFDNLWALGQRLIRKGEFDKYLTR
                     PISPLFHVLVETFQVDALGELLVGFILLSTTVSSISWTVPKVLLFIFIIPFATLIYTS
                     LKIATSSIAFWTKQSGAVIYIFYMFNDFAKYPVAIYNNLLRWIISFVIPFAFTAYYPA
                     AYFLQDRNVYFNIGGVILISLISFMVSLILWHKGVEVYESAGS"
     gene            complement(1088..1906)
                     /locus_tag="ACUBV3_001895"
     CDS             complement(1088..1906)
                     /locus_tag="ACUBV3_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HHU3848367.1"
                     /translation="MNRIWRRYKPFISTGIQGLITYRVDFILYRIGDVIGAFVAFYLW
                     KAVFDSSSQSLIQGFQLSDMILYIIMSFVTNLLTRTDSSFMIGDEVKDGSIIMRLLRP
                     VHFAASYLFMEIGSRWLIFLSIGVPFLLVITGVRLFLGTDLIQAIVLVVFYIISIILA
                     FLINFFFNICFGFSAFVFKNLWGSNLLKNSLVAFMSGSLIPLTFFPKIVADILGFLPF
                     SSLIYTPVMIIIGKYDGSQIVQALLLQIFWLIVMVALSQLIWKKVQSHITIQGG"
     gene            complement(1909..2901)
                     /locus_tag="ACUBV3_001896"
     CDS             complement(1909..2901)
                     /locus_tag="ACUBV3_001896"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HHU3848368.1"
                     /translation="MSMIEVSHLQKNFIKTVKAPGLKGAFQSFLRPEKHTFEAVKDLT
                     FDVPKGQILGFIGANGAGKSTTIKMLTGILKPTSGFCRIDGKLPQENRQNYVKDIGVV
                     FGQRTQLWWDLALQETYTVLKEIYDVPDKEFRKRMAFLNEVLELNDFIKDPVRTLSLG
                     QRMRADIAASLLHNPKVLFLDEPTIGLDVSVKDNIRRAITQINQEEETTILLTTHDLS
                     DIEQLCHRIFMIDRGQEIFDGTVSQLKETFGKMKTLSFDLRPGQEHISSSLIGKSEIN
                     IKRNDLVLDIQYDSSRYQTADIIQQTLADFSVRDLKMTDADIEDIIRRFYRKEL"
BASE COUNT         1102 a          562 c          461 g          915 t
ORIGIN      
        1 tttcataaat gacatctgct ttttgcttac cttttataga ccaaaaaacg ctcttagaaa
       61 gcacatctaa gagcgttttt aactttattt tggtaaagat atggtaatta ttgttctatg
      121 agggacatta ttctcaacac taatagtccc tcttcacctg atatactccc cttatagtgg
      181 acagtaaaaa aacaaaaatt cactagacac tataagggga gtatatcatc actaacaaag
      241 ctaagttagt aagcctaaaa cttctaaaca ctcaaaaaaa ttaatggctt gccttactaa
      301 tcaagaaccc gcactctcat atacttctac tcctttatgc caaagtatta gtgataccat
      361 aaaagaaatc aaggaaatca agataacccc accaatatta aaatagacat ttctatcttg
      421 taagaagtaa gcagcgggat aataagctgt aaaagcaaat ggaatgacaa aactaatgat
      481 ccaacgcaat agattattat atattgcgac aggatactta gcaaagtcat taaacatata
      541 aaaaatatag ataacagcac cagattgttt ggtccaaaaa gcgatacttg acgtagcaat
      601 cttcaaggag gtataaatca aagttgcaaa ggggataata aatataaata ataatacttt
      661 tggcacggtc cagcttatac tagatactgt ggtagataac agaataaaac caaccaataa
      721 ctcccccaat gcatctactt gaaaggtttc aactaggaca tgaaataaag gactgatagg
      781 cctggttaaa tacttatcaa attctccttt tcgaataagc ctttgaccaa gagcccacaa
      841 gttatcaaag aaaaggtgat caattccttt tgggaggaga gaaaaaccat aaataaaagc
      901 tatttgctga aaagtccatc cttctaaaga agggatatgt tgaaaaagaa cattcagaaa
      961 taaaagattt agcccttgtg ttaacaatac tcctaaaacg cctacaaaaa agtctatttt
     1021 atactccatg atttgcttca tgtattgttt aataaaaata aagtgcattc gctgatattt
     1081 tgtcatctca acctccttgg atagtgatat gcgactgtac ttttttccat attaattgag
     1141 ataaagctac cattactatt agccaaaata tttgtaaaag taaggcttgc acaatttgac
     1201 tgccatcata tttaccaata ataatcatta ctggtgtgta aattaaagat gaaaatggta
     1261 aaaatcctaa aatatcagca acgattttcg gaaaaaaagt aagaggaatc aagctccccg
     1321 acataaatgc gactaacgaa ttttttaaca ggtttgaccc ccataaattt ttaaaaacaa
     1381 atgccgagaa gccaaaacaa atattaaaaa agaagttaat caaaaaagct aaaataatgc
     1441 taataatata aaataccact aacactatag cttgaatcaa atcagtcccc aagaagaggc
     1501 gtacccccgt aataacaagc aaaaacggta cccctatact taaaaagatg agccatctag
     1561 aaccaatctc catgaaaagg taggaagcag caaaatgaac tggacgtaag agtcgcataa
     1621 taattgaacc gtcttttact tcgtcaccta tcataaatga actatcagtt cttgttaata
     1681 aattagtcac aaaactcata ataatataga gaatcatatc tgacagctga aaaccttgga
     1741 ttaaggattg acttgaggaa tcgaaaactg ctttccatag ataaaatgca acaaatgctc
     1801 ctatcacatc acctatccta taaaggatga aatcaacacg atatgtgatt aatccttgga
     1861 ttcctgtact aataaaaggt ttataacgtc tccaaattct gttcatcctt atagctcctt
     1921 tctatagaag cgacgaatga tatcttcaat atccgcatct gtcattttaa ggtcacgtac
     1981 agaaaaatct gctaatgtct gctgaattat gtcagcagtt tggtagcgag aactatcata
     2041 ctgaatatct aaaactaagt cattacgttt aatattaatc tccgactttc caattaaact
     2101 agagctgata tgctcttgtc ctgggcgcaa atcaaaagaa agagttttca tctttccaaa
     2161 tgtctctttt agctgagaaa ctgtgccatc aaatatttct tgtcctctat caatcataaa
     2221 aatacgatga caaagttgtt caatatcact gaggtcatgt gtcgttagta aaatcgttgt
     2281 ctcttcttct tgattaattt gtgtgattgc acgtcggata ttatctttca ctgaaacgtc
     2341 caagccaatg gtgggttcat ctaagaaaag aacctttggg ttatgtaata aagaagctgc
     2401 gatatccgct ctcatacgct gtccgagaga caaagttcta acaggatcct ttataaagtc
     2461 atttagttcc aaaacctcat ttaaaaaagc catgcgtttg cgaaattctt tatcaggaac
     2521 gtcatagatt tcttttaaca ctgtataggt ttcttgtaga gctaaatccc accacaactg
     2581 cgtacgttgc ccaaaaacga ctccaatatc cttaacataa ttttgtcggt tttcttgtgg
     2641 caatttgcca tcaatacggc aaaagcccga agttggtttt aaaatcccag ttaacatttt
     2701 aattgttgta gattttcctg caccgttcgc tccaataaat cccaaaattt gtcctttagg
     2761 cacatcaaat gttaaatctt taacagcttc aaaggtgtgt ttttcaggac gaagaaatga
     2821 ttgaaatgct ccttttaacc ctggtgcttt aacggttttt ataaaatttt tctgaaggtg
     2881 agaaacttca atcattgaca tattaatacc tcttttactc aacagtattt ttatagctta
     2941 aatcagctcg acagttagat tattaaacta taataatgat taacagctat ttattatagc
     3001 acaattaaaa attcaatgca aacgttttct tagatggtgt
//
LOCUS       DBJYSH010000153         2968 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.153, whole genome shotgun sequence.
ACCESSION   DBJYSH010000153 DBJYSH010000000
VERSION     DBJYSH010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2968
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.153"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            complement(<1..1091)
                     /locus_tag="ACUBV3_001897"
     CDS             complement(<1..1091)
                     /locus_tag="ACUBV3_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HHU3848369.1"
                     /translation="MHIIIQITIILLASVLATLISKRIGIPAVVGQLLVGIIIGPAML
                     GLVHQNQVLHVLSEIGVILLMFLAGLEANFDLLKKYLKPSLLVAITGVIVPMALFYFL
                     TRLFGFQINTAIFYGLVFAATSISITVEVLQEYNRVKTDTGAIILGAAVADDVLAVLL
                     LSVFIATNGSSSNIGLQIIIQLLFFVFLFICMKYLVPALFKLIEKVHFFEKYTILAIL
                     ICFSLSILADKVGMSSIIGSFFAGLAIGQTSFVDKVEHKISLLSYTFFIPIFFASIAL
                     PLKFDGMMSHLHTILIFTALAVLSKLIPGYFVGRGFNFSKLESLTIGGGMVSRGEMAL
                     IIVQVGLAAKIISSTTYSELVIVVILSTI"
     gene            complement(1361..2923)
                     /gene="guaA"
                     /locus_tag="ACUBV3_001898"
     CDS             complement(1361..2923)
                     /gene="guaA"
                     /locus_tag="ACUBV3_001898"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HHU3848370.1"
                     /translation="MTDISILNDIQKIIVLDYGSQYNQLIARRIREFGVFSELKSHKI
                     TADEIRDINPIGIVLSGGPNSVYADGAFGIDEEIFELGIPILGICYGMQLITHKLGGK
                     VLPAGEAGHREYGQSALRLRSESALFAGTPQEQLVLMSHGDAVTEIPEGFHLVGDSVD
                     CPFAAMENTEKQFYGIQFHPEVRHSVYGNDILKNFAVNICGARGDWSMDNFIDMEIAK
                     IRETVGDRKVLLGLSGGVDSSVVGVLLQRAIGDQLTCIFVDHGLLRKNEGDQVMDMLG
                     GKFGLNIIRVDASKRFLDLLSGVEDPERKRKIIGNEFVYVFDDEASKLKGVDFLAQGT
                     LYTDIIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRALGTALGMPDEV
                     VWRQPFPGPGLAIRVMGEITEEKLETVRESDAILREEIAKAGLDRDVWQYFTVNTGVR
                     SVGVMGDGRTYDYTIAIRAITSIDGMTADFAQLPWDVLKKISTRIVNEVDHVNRIVYD
                     ITSKPPATVEWE"
BASE COUNT         1054 a          568 c          505 g          841 t
ORIGIN      
        1 ataatagttg acagtataac aacaataaca agctcggaat aagtagtgct tgaaataatt
       61 ttagcagcta atccaacttg aactataatg agagccatct cgccacgact gaccattcca
      121 ccaccaattg ttaaagattc taattttgaa aaattaaagc ctcgaccgac aaagtaccct
      181 ggaattaatt ttgataaaac agccaaagca gtaaagatta atatcgtgtg aaggtgagac
      241 atcattccat cgaatttaag aggtagtgca attgaagcga agaaaattgg gataaagaag
      301 gtgtaactaa gaagtgaaat tttatgttct accttatcaa caaaagaagt ttgtccaata
      361 gctaatcctg caaaaaacga accaatgata gaactcattc caactttatc ggctaaaatt
      421 gatagagaaa aacagataag gatagcaaga atagtatatt tctcaaagaa atgaaccttt
      481 tcgattaatt tgaaaagagc aggtacaagg tatttcatac aaataaataa gaaaacaaag
      541 aataacaatt gaatgataat ttgtaatccg atattagaag agcttccatt tgttgcaata
      601 aagacactca gaagcaaaac agctaagaca tcatcagcaa cagcagcacc taaaataata
      661 gcaccagtat ctgtttttac acgattgtat tcttgtaaaa cttcaactgt gatagaaata
      721 ctagttgcag caaatacgag cccgtaaaag atagcagtat tgatttggaa cccaaacaag
      781 cgtgtcaaga agtagaagag agccatagga acaataacac cagtaattgc gaccagtaga
      841 ctgggcttaa gatacttctt taaaagatca aaattagctt caagacccgc taaaaacatg
      901 agtaagataa caccaatttc tgacaggaca tgtaatacct gattttgatg aacaagtcct
      961 agcattgcag gtccgatgat gatacctaca agtagttgtc caacaacagc cggtattcca
     1021 attctttttg aaataagagt tgcaagaacg cttgccaaca aaatgattgt aatttgaatg
     1081 ataatatgca ttttttcctc ctaatattaa taaaatgata tcaatcatat tgtactgaat
     1141 atttagaata ccatttttta agcctaaaat ctaggaatat ttcttttctg tctttttcat
     1201 attattatca agaaaatgga aaaatatact gaaaaatatt caatttaaga ataatgaact
     1261 tgtaactatc gaactacata aaaatgatat tttaagtgta agtatccctt attcaagaaa
     1321 taagagtaaa aaaacttagc tacttaaagt agctaagttt ttattcccac tcaacagttg
     1381 caggtggttt acttgtaata tcataaacaa tacggttaac gtgatcaact tcgttaacaa
     1441 tacgtgtcga aattttctta agtacatccc aaggaagttg cgcaaagtca gctgtcatgc
     1501 catcaattga agtaattgca cggatagcaa ttgtatagtc ataagtacga ccatctccca
     1561 taacaccgac agaacgtacg ccagtattaa ctgtgaaata ctgccatacg tcacgatcta
     1621 gaccagcttt agcgatttct tcacgaagaa tagcatctga ttcgcggact gtttcaagtt
     1681 tttcttcggt aatttcaccc ataacgcgaa tagcaagacc aggcccaggg aatggttgac
     1741 gccatacaac ttcatcaggc attccaagtg cagtaccaag agcgcgaacc tcatctttga
     1801 ataatgtgtt aagtggctca atcaattcaa attgcatatc ttctggcaat ccaccaacat
     1861 tatggtgtga cttgattgtt tgggctgttt ctgtcccaga ttcaataata tctgtgtaga
     1921 gggtaccttg agcaaggaaa tcaacgcctt taagtttact tgcctcatca tcaaaaacgt
     1981 acacgaactc atttccgatg atttttcgtt ttctctcggg atcttcaaca ccagaaagaa
     2041 gatctaggaa gcgttttgaa gcatcaacac ggataatatt aagtccaaat ttaccaccaa
     2101 gcatatccat aacttggtca ccttcatttt tacgtaataa accatgatct acaaaaatac
     2161 atgttaattg atcaccgata gcacgttgta ggagtactcc gacaacagat gaatcaacac
     2221 cgccagatag tccaagaagt actttacgat cgcctacagt ttcacgtatt tttgcaattt
     2281 ccatatcaat gaagttatcc attgaccaat caccacgtgc accacagata ttaacagcaa
     2341 aatttttaag gatatcattg ccatatactg agtgacgaac ttcggggtgg aattggatac
     2401 cataaaattg tttttcagta ttttccattg cagcaaatgg acagtcaaca gagtcaccaa
     2461 cgagatggaa accctcaggg atttcagtaa cagcatcacc atggctcatc aaaacaagtt
     2521 gttcttgagg agtgccagca aagagtgctg attctgagcg taggcgaagt gctgattgac
     2581 catactcacg gtgaccagct tctcccgcag gaagaacctt accaccgagt ttatgggtaa
     2641 tcagttgcat accataacaa attcctaaga ttggaattcc taattcaaaa atttcttcat
     2701 cgattccaaa agcgccatca gcgtatacgg aatttggacc acctgaaagt acgataccaa
     2761 taggattaat atcgcgaatt tcatcagccg taattttatg tgattttaac tcagaaaata
     2821 caccaaattc acggatacga cgtgcgataa gctggttata ttgactaccg taatctagaa
     2881 cgataatttt ttggatatca ttcaatatag aaatatcagt cattgtttcc ctttctttgt
     2941 agctaatttt aaaaacagct gtacctat
//
LOCUS       DBJYSH010000154         2851 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.154, whole genome shotgun sequence.
ACCESSION   DBJYSH010000154 DBJYSH010000000
VERSION     DBJYSH010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2851
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.154"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            complement(9..239)
                     /locus_tag="ACUBV3_001899"
     CDS             complement(9..239)
                     /locus_tag="ACUBV3_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1797 family protein"
                     /protein_id="HHU3848371.1"
                     /translation="MESHLVRIINRLELMATDGGNLKRNFEREGVVVAEVSFSNDPEN
                     GPVFTLRDVEARESYSFDSIDLIAMEIYDLLY"
     gene            537..2798
                     /locus_tag="ACUBV3_001900"
     CDS             537..2798
                     /locus_tag="ACUBV3_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HHU3848372.1"
                     /translation="MLCQNCKLNESTIHLYTNVNGKQKQVDLCQNCYQIIKTDPNNPL
                     FSGLNHVSHAPGGINPFFDDFFGDLNNFRAFNGQDLPNTPPTQSGGNRGGGNGNGRNN
                     NRNQTATPPQAKGILEEFGINVTEIARHGDIDPVIGRDSEIIRVIEILNRRTKNNPVL
                     IGEPGVGKTAVVEGLAQKIVDGNVPHKLQGKQVIRLDVVSLVQGTGIRGQFEERMQKL
                     MEEIRQRQDVILFIDEIHEIVGAGTAGEGSMDAGNILKPALARGELQLVGATTLNEYR
                     IIEKDAALERRMQPVKVDEPSVEETITILKGIQKKYEDYHHVKYNNDAIEAAAVLSNR
                     YIQDRFLPDKAIDLLDEAGSKMNLTLNFVDPKEIDQRLIEAENLKVQATREEDYERAA
                     YFRDQIAKYKEMQQQKVDDQDTPIITEKTIEHIIEEKTNIPVGDLKEKEQSQLINLAD
                     DLKQHVIGQDDAVIKIAKAIRRNRVGLGSPNRPIGSFLFVGPTGVGKTELSKQLAIEL
                     FGSADSMIRFDMSEYMEKHAVAKLVGAPPGYVGYEEAGQLTEKVRRNPYSLILLDEIE
                     KAHPDVMHMFLQVLDDGRLTDGQGRTVSFKDTIIIMTSNAGSGKTEASVGFGASREGR
                     TNSVLGQLGNFFSPEFMNRFDGIIEFKALDKENLLNIVDIMLSDVNARLAINGIHLDV
                     TDKVKEKLVDLGYDPKMGARPLRRTIQEHIEDAITDYYLENPSEKELRAIMTSNGNII
                     IKSSKKTEESAKG"
BASE COUNT          966 a          522 c          522 g          841 t
ORIGIN      
        1 tataattatc agtataataa atcgtaaatt tccattgcga taaggtcaat gctgtcaaat
       61 gaatatgatt cacgcgcttc tacatcacgt aatgtgaaaa caggaccgtt ttcagggtca
      121 ttactaaaag atacttctgc tacaacaaca ccttcacgtt caaaattacg ttttaaatta
      181 ccaccatcag tcgccattaa ttcgaggcga ttgatgattc tcactaaatg tgattccatc
      241 ttacttactc ctatatattt tctagtcctt tctattttaa cacaaaagtg accaaagcaa
      301 catgaaaagc aacattttta ctaacttttt aacgatttta tcgtctaaaa tccgcaaata
      361 acgatttttc tcagtagtca tctatttttc aatttagcac tctaatatat tcagtgctaa
      421 aaacagaact tttttcttga tttctaggct ttaggtgcta taataaaagc ataaagtttg
      481 actatatttg actatataca catcaatagt taaatgtcat aagaaagggg tagcctatgc
      541 tctgtcaaaa ctgcaaacta aacgaatcaa ctattcattt gtatacaaat gttaatggaa
      601 aacaaaagca agttgatctt tgtcaaaatt gctaccaaat tattaaaact gaccctaata
      661 atccactttt ttcaggttta aatcatgttt cacatgcgcc tggtggcatc aatcctttct
      721 ttgatgattt ctttggagat ttaaataatt tcagagcctt taatggccaa gatttaccaa
      781 acactcctcc tacacaatca ggaggaaacc gaggtggagg aaacggtaat ggacgaaata
      841 ataatcgcaa ccaaactgca actcctcctc aagccaaagg gattctagaa gaatttggta
      901 tcaatgtcac tgaaatagcc cgccatggtg atattgaccc tgttattgga cgtgactcag
      961 aaattattcg tgttatcgaa atccttaatc gtcgtacaaa aaataatcct gtacttattg
     1021 gtgaacccgg cgtagggaaa actgctgttg ttgaaggact tgctcagaaa attgttgatg
     1081 gtaatgtgcc tcataaactt caaggcaaac aagttattcg tttggatgtt gtgagtctag
     1141 tacaaggaac gggcattcgt ggtcaatttg aagaacgcat gcaaaagttg atggaagaaa
     1201 ttcgtcaacg tcaagatgtt attctattca tagatgaaat tcacgaaata gttggagctg
     1261 gtacagctgg cgaaggtagt atggatgcag gtaatatttt aaaacctgcc cttgcacgtg
     1321 gagaattaca actagtaggt gctactactc tcaatgaata tcgtattatc gaaaaggacg
     1381 ctgccttaga acgccgtatg caacctgtaa aagtcgatga gccttctgtt gaagaaacca
     1441 ttactatttt gaaaggtatc caaaaaaaat acgaagatta tcatcacgta aaatataata
     1501 atgatgccat agaagcagct gcagtactat ctaatcgtta tatccaagac cgctttttac
     1561 ctgataaagc aatagactta ttagatgaag ctggttctaa aatgaaccta acactaaatt
     1621 ttgttgatcc aaaagaaatt gatcaacgtc tcattgaagc agaaaattta aaagtgcaag
     1681 cgactcgtga agaagattac gaacgtgcag cttacttccg tgaccagatt gcaaaatata
     1741 aagaaatgca acaacaaaag gtcgacgatc aagatacacc tattattacc gaaaaaacaa
     1801 ttgagcacat cattgaagaa aaaacgaata tccctgttgg tgatttaaaa gaaaaagaac
     1861 aatctcaatt aattaatctc gcagatgact tgaaacaaca tgtgatcggc caggatgacg
     1921 ctgtcattaa gattgcaaaa gctattcgtc gtaatcgtgt tggtcttggt agcccaaacc
     1981 gtcctattgg ttccttttta tttgtaggac caaccggtgt tggtaaaact gaactttcta
     2041 aacaactagc aattgagctc tttggttcag ctgatagtat gattcgtttt gatatgtcag
     2101 agtacatgga aaagcatgct gttgctaaat tagtcggagc gcctccagga tacgtgggat
     2161 acgaggaagc tggacaatta actgaaaagg ttcgtcgaaa tccttactcg ctcatccttc
     2221 tagatgaaat tgaaaaagct catcctgatg tcatgcatat gttcttgcag gtccttgatg
     2281 acggtcgatt aacagatgga caaggaagaa ctgttagttt taaagatacc attatcatca
     2341 tgacctcaaa tgctggttct ggtaaaactg aagcaagcgt tggctttggt gcctcacgag
     2401 aaggtaggac gaattccgta ctaggtcaac taggtaactt cttcagccct gaatttatga
     2461 accgctttga cggtattatt gaattcaagg ctttagataa agagaatctc ctcaatatcg
     2521 ttgatattat gttatctgac gttaatgcac gtctcgccat caatggtatt catctagatg
     2581 tcactgataa agtgaaagaa aaattggttg atttaggtta cgatccaaaa atgggagcac
     2641 gtccattacg tcgtaccatt caagaacata ttgaagatgc tatcacagat tactatcttg
     2701 agaatccaag cgaaaaagaa cttcgtgcta ttatgactag caatggaaat atcataataa
     2761 aatcttctaa aaaaactgag gaaagtgcaa aaggttgagt aagaaactca accttttttc
     2821 acactcttct caaaaaataa tagcctctat a
//
LOCUS       DBJYSH010000155         2749 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.155, whole genome shotgun sequence.
ACCESSION   DBJYSH010000155 DBJYSH010000000
VERSION     DBJYSH010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2749
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.155"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            complement(77..1459)
                     /locus_tag="ACUBV3_001901"
     CDS             complement(77..1459)
                     /locus_tag="ACUBV3_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HHU3848373.1"
                     /translation="MLTTKENFKGNNKLLVGIVLAVLSFWLFAQSILNMGPDVQSSLG
                     ISSGAMDIGVSSTALFSGLFIVVTGGLADKLGRVKFTFIGLCLNIIGSLLIVLANGAV
                     LFIMGRIFQGLAAAFIMPSTMALVKTYYDGKDRQRAVSFWSIGSWGGSGLCSYFGGAV
                     ASTLGWRYVFIFSIIASVVSFLLILGTPESKNVGQKTHFDYLGLIIFIISMLSLNIGI
                     SMAQEHGLMNVIPLSLFTVMLIGFVLFYYVETRKSNSFIDFHLFENRFYLGATISNFL
                     LNAVAGTLIVINTYMQQGRQLTPKVAGEMSLGYLVCVLIAIRVGEKILQRFGARKPML
                     LGAMSTFVGIFLMTLVNIQGPLYLVLVFVGYALFGTGLGIYATPSTDTAISSIPNEKV
                     GSASGIYKMASSLGGAIGVATSIAIYHAFSGNADFHKAALCGLILNLVFCSLSILSIL
                     FVIPKKIEDK"
     gene            complement(1467..2681)
                     /locus_tag="ACUBV3_001902"
     CDS             complement(1467..2681)
                     /locus_tag="ACUBV3_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HHU3848374.1"
                     /translation="MANASLRHQLFEKLDQKCDQMVAIRRYLHENPELSFKETKTAAY
                     ISDFYKGKDCHVQTQFGGMNGVVVDIYGDKATDKPIKHIALRADFDALPIQEETGLSF
                     ASKTAGVMHACGHDAHTAYLLILAESLIELKSEFSGHIRILHQPAEEVPPGGAKAMIE
                     AGCLDGIDAVLGIHVMSTMEEGTVQYHAGPIQTGRATFKVILQGKGGHGSMPHRANDT
                     IVAASSFVMAAQTIVSRRVNPFDTAVVTIGSFDGKGSANVIKDSVTLEGDVRVMSEET
                     RGVVEEEFKRILDGIAQTYGVSYQLDYQNDYPVLVNNSEVTQKVANSLKSVAIKEILD
                     VIDCDPQTPSEDFAYYAQTIPACFFYVGAHEEGQPYYPHHHPKFQIAESSLMVSAKSM
                     ATAALAMLVEGE"
BASE COUNT          936 a          580 c          423 g          810 t
ORIGIN      
        1 tattttaaat ttctttccca attcctttta cattattaca acaatacttg agattttgaa
       61 tctcaagtat tgttatttat ttatcctcaa ttttcttagg gataacaaaa agaattgata
      121 agatcgatag actacaaaaa actaggttta aaattaatcc acacaaagct gctttgtgaa
      181 aatctgcatt accagaaaaa gcatgataaa tcgcaattga agttgctaca ccaattgcac
      241 cacctagtga acttgccatt ttgtaaattc cagatgcaga acctactttt tcattaggaa
      301 tactagaaat agctgtatct gttgatggtg tcgcatagat ccccaagcct gttccaaata
      361 aagcataacc aacaaagact aacacaagat acaaaggtcc ttgaatattg accaaagtca
      421 ttaaaaatat cccaacaaat gtactcatag ctcccaaaag cattggctta cgggcaccaa
      481 aacgttgtaa aattttctca ccaactcgga tagcaattag tacacaaacc aaatagccta
      541 aagacatttc acctgcaacc tttggtgtta gctggcgacc ttgttgcatg taagtattaa
      601 taacaatcag agttcctgca actgcattca gtaagaaatt tgatattgtc gcacctaagt
      661 aaaatcgatt ttcaaacaga tgaaagtcaa taaaactatt agatttacgt gtttcaacat
      721 aataaaacag aacaaatcca ataagcatca ctgtaaaaag actcaatgga ataacgttca
      781 tcaatccatg ttcctgagcc atagatatcc caatatttaa cgataacatt gaaataataa
      841 aaataattaa accaaggtaa tcaaagtgag ttttttgtcc aacattttta ctttctgggg
      901 ttcctaaaat taatagaaaa ctaactactg atgcgatgat tgaaaagata aagacataac
      961 gccaacctag agtacttgcc accgctccac caaaataaga acatagacct gagccacccc
     1021 acgatccgat tgaccagaaa ctaactgcac gttgtctatc tttaccatca tagtacgttt
     1081 taaccaaggc cattgttgat ggcataataa aagctgctgc caatccttga aaaatacgtc
     1141 ccataataaa tagcactgct ccattagcta aaacaattag tagagaacca ataatattta
     1201 agcaaagacc aataaaagta aatttaactc gaccaagttt atctgctaaa ccaccagtta
     1261 caacaataaa taaacctgaa aacaatgccg tacttgagac accaatatcc attgcaccag
     1321 atgaaatacc taatgatgac tgaacatctg gtcccatgtt taaaatagac tgtgcaaata
     1381 accaaaatga tagtacagcg agtacaattc ccaccaataa tttgttattt cctttgaaat
     1441 tttcctttgt cgttaacata tgttatctat tctccttcta caagcatagc aagcgctgct
     1501 gttgccattg atttagcaga taccatcaat gatgattctg caatttggaa ttttggatga
     1561 tgatgtgggt aatatggttg cccctcttca tgtgctccaa catagaagaa gcaagctggg
     1621 attgtttgag cataataggc aaaatcttca gacggcgttt gagggtcgca atcaatgaca
     1681 tctaaaattt cttttatagc aacagatttt aaactatttg caactttctg tgtcacttca
     1741 ctgttattaa ctaaaacagg ataatcattc tgatagtcta actgataact aacaccataa
     1801 gtttgtgcta ttccatctag aatacgctta aattcctcct caacaacacc tcgtgtctct
     1861 tcagacatca cgcgcacatc accttctaag gttacactat ctttgatcac attagcagaa
     1921 cctttaccat caaaagatcc gatagtaact actgctgtgt caaatggatt aactcgacga
     1981 ctgacaatcg tttgcgctgc cataacaaaa ctagaagctg caacaattgt atcatttgca
     2041 cgatgtggca tggaaccatg tccaccctta ccttgcaaaa taactttaaa agtggcacgc
     2101 cctgtttgaa tgggacctgc gtgatattgt accgttccct cttccatcgt tgacataaca
     2161 tggataccaa ggacagcatc aatgccatct aaacatcctg cttcaatcat tgcttttgct
     2221 ccgcctggtg gcacctcttc agccggttga tgtaagatac ggatatgacc tgaaaattct
     2281 gattttaatt caatcaagct ctcagccaaa atcaataaat aagctgtatg ggcatcatgt
     2341 ccacaagcat gcatgacgcc cgctgttttt gaagcaaaag aaagccctgt ctcttcctga
     2401 attggaagag catcaaagtc ggctctcaaa gcaatgtgtt tgatgggctt atctgtcgct
     2461 ttatcaccat agatatcaac tacgacgcca ttcataccac caaattgtgt ctgaacatga
     2521 cagtccttac ctttataaaa atctgaaata taagctgctg tttttgtctc tttaaatgat
     2581 aattcgggat tttcatgtaa atagcgacga atagcaacca tttgatcaca tttttggtca
     2641 agtttttcaa ataattgatg tcgcaaagat gcattggcca tttgctttcc tccttaaaat
     2701 atgaacagtt aaaataaggc tattattcta acaatctgct cttatatag
//
LOCUS       DBJYSH010000156         2680 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.156, whole genome shotgun sequence.
ACCESSION   DBJYSH010000156 DBJYSH010000000
VERSION     DBJYSH010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2680
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.156"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            <1..84
                     /locus_tag="ACUBV3_001903"
     CDS             <1..84
                     /locus_tag="ACUBV3_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000965641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Paratox"
                     /protein_id="HHU3848375.1"
                     /translation="YVLPHEEVRDDEVVTVESVEEVLRELK"
     gene            complement(365..754)
                     /locus_tag="ACUBV3_001904"
                     /pseudo
     CDS             complement(365..754)
                     /locus_tag="ACUBV3_001904"
                     /inference="COORDINATES: protein motif:HMM:NF012798.6"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(790..1056)
                     /locus_tag="ACUBV3_001905"
     CDS             complement(790..1056)
                     /locus_tag="ACUBV3_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001881173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HHU3848376.1"
                     /translation="MAYFRKRDNGWEYRISYKHIIGNILLCNITSTKYQQVINHYSQT
                     HVQDTVKRFNIHVKACVSMAVHEGYIEKNFCLFTKVKAKNKGRK"
     gene            1062..2439
                     /locus_tag="ACUBV3_001906"
                     /pseudo
     CDS             1062..2439
                     /locus_tag="ACUBV3_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611955.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(2612..>2680)
                     /locus_tag="ACUBV3_001907"
     CDS             complement(2612..>2680)
                     /locus_tag="ACUBV3_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NINE protein"
                     /protein_id="HHU3848377.1"
                     /translation="IDLIEALMALGKQTDEYGNIIV"
BASE COUNT          846 a          449 c          525 g          860 t
ORIGIN      
        1 tatgtgttgc cgcacgagga agtgagagat gatgaagttg tgacagttga gagtgtagaa
       61 gaggtgttga gggaattgaa gtgaaagggt gcaaaaaagg ggcaaaatag attaaaaatg
      121 aaactacatt gaacttttta ctattataaa caaccattaa atcaacgctt ggactattat
      181 gaactattgt gtacaatagt agtagattcc ggcaggggac atattcattg catgtaaata
      241 gcggtttaga gctattttgc cccaaatttc tctgattaag tttatcgttc ctatcttttt
      301 gttcttgtaa ttgatgtgcg taaacttcta aagtgatatt taaattctcg tgatctaaaa
      361 cttgagagat ggaaattaga tagcttgcaa atgtatgcct gagagagtgc actcgtacct
      421 cgcgaccagt tatttttcgg atagttttat tgactgcatt atttgaaagt ttgtcgaata
      481 atctgtcgtt tttatttttt gtaaattcat gcaaaaaaaa taatgtatca ttgtcaattg
      541 gtatatttct gatactactt ttgttttttg ttggcaggta tctttggttg aaatgataat
      601 cccaagtttt attaattgat aaatatttgt tagtgtaatc aatatcatta actgttaaac
      661 ctaaacattc agcgaagcgc atgccagttt tagcgatgag gtataacgct gcatacgatt
      721 gatgttgtga tttttcttta caaattttta tcaagcgtaa gtattcattg gtttcaagaa
      781 attttatctc tatttacgcc ccttattttt tgctttaacc ttagtgaata aacaaaaatt
      841 tttttctata tatccctcgt gaacagccat ggatacgcag gcttttacat gtatgttaaa
      901 acgctttact gtatcttgca catgcgtttg actataatga tttatgactt gttgatattt
      961 agtggaagta atattgcaaa gtaatatatt tcctattata tgtttatacg atattcgata
     1021 ttcccacccg ttgtcgcgtt tacggaaata cgccattgat atactccaca ttagctaaag
     1081 aacagggtgt tcaaggctac cttgatggaa aaggctctct tagagatatt tgtaaatggt
     1141 atgatatctc aagtcgctct gttctccaaa agtggataaa acggtatact agtggtgaag
     1201 acttgaaagc cactagtaga ggatatagcc gtatgaaaca aggaaggcaa gccacatttg
     1261 aagaacgtgt agagattgtt aactacacca ttgcccatgg gaaagactat caagcagcta
     1321 ttgagaagtt tggtgtttcc taccaacaaa tttattcttg ggtgcgtaag cttgagaaga
     1381 atggctcaca aggtttggtt gatagacgtg tgaaagggtt ggagagtagg cctgatttaa
     1441 ccgagattga gcaactttaa ctcaagatta aacaattgga ggaacgtaat cgtctcttag
     1501 aaatcgaggt tagtttacta aaaaagttag aagacatcaa acgaggaaac agacggtaag
     1561 actaggtaag catttagcgg agttccaagt aatcaagaat tattacgatg aggaatctaa
     1621 tgtgcctatt caggccttat gccaactctt gaaggggtct cgttcaggct attacaagtg
     1681 gctcaatcgt caaaaaacag attttgagac aaaaaataca aagctaatgg ctaaaatcaa
     1741 ggaacttcgt agactctaca atggtatctt aggttatcgc cgtatgacaa catttattaa
     1801 tcgtcaactt gggacaactt aaaacaagaa acggattcgt tgattgatga acattctggg
     1861 gattagttca gtcattcgtc gtgttagcca tgcttgtaca aaagctggtg acagatttta
     1921 cgaagaaaat attcttaatc gtgaatttac agccacagct cataaccaga aatggtgcac
     1981 agatgtcacc tatcttcaat acggtctggg agctaaagct tatctcagtg cgattaaaga
     2041 cctgtataac ggttctatta tcgcttatga gattagtcac aacaatgaaa tccacttgtt
     2101 atgaagacca ttaaaaaggg gctagagctc aatccaggag ccacacctat catccatagc
     2161 gattgaggta gtcaatatac ttccaaagaa taccgttata tcatacaaca agctggtctg
     2221 accttatcca tgtcccggat tggcaaatgt attgataatg caccaactga aagtttcttt
     2281 gggtttttca agactgagtc ttaccacctt aagaaataca actcttatga tgagttggtc
     2341 aatgatgtgg cacgttatat cgaattctac aacacacaac gttatcaatc aaaattaaac
     2401 aacctgactc ctctagaatt caggaatcag gttgcataac ttatctttta ttatttgact
     2461 gtctacttga cagggagccg ttcagattgc ttaacctttc taaatttgct aaaatagcta
     2521 caagaaaacg agccatttaa tgcttatttc ttatactgtc ttgcctcacg ctctcctcga
     2581 ccaaaaattg agcgtgaggc tttttgtttc attaaacgat gatatttcca tattcatcag
     2641 tttgttttcc gagagccatc aaagcttcga taaggtcgat
//
LOCUS       DBJYSH010000157         2619 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.157, whole genome shotgun sequence.
ACCESSION   DBJYSH010000157 DBJYSH010000000
VERSION     DBJYSH010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2619
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.157"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            complement(<1..552)
                     /locus_tag="ACUBV3_001908"
     CDS             complement(<1..552)
                     /locus_tag="ACUBV3_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HHU3848378.1"
                     /translation="MIKKNKVFLGVLLVLVVILGGGVLFYQSQFQKTTNQALAIAYKD
                     AKVAKKDVIHQKIDKEFENFRGSYEIEFNTKSAEYSYHVDAKTGQILERDMDNNGFSK
                     STSQSSSSSSQKSHKISQEEAKKIAFKDANIEESEVSNLKIKEEIENGKSVYDIDFVD
                     LKNKNEVDYQIDAETGKIIERSRD"
     gene            complement(719..1738)
                     /locus_tag="ACUBV3_001909"
     CDS             complement(719..1738)
                     /locus_tag="ACUBV3_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3114 domain-containing protein"
                     /protein_id="HHU3848379.1"
                     /translation="MAKTALNIPFTVEGIENYYQNIKMILSLADKWSIPKDDLLLFLK
                     GLKAIDDIKVGSPIFKYFWTSLSLQAPLKALEFVLEQAKMPTELSGELSETQYLVAQF
                     SDELAPHDDFWIALSQVIYDSFPGNSLAEDTVLNRKLHQFRYLISSQQAQYVRRYFKD
                     VGMTDRDALVNYLSCLREPDSIAYYESARLHNKRRRNGEIFGFPDDEPVINSKLLISF
                     HTEFIIDDKGNFLNEIDAEVITRNGIINGASFNYAFKNNTRHKELDVDPVKLDPKFRN
                     DMTRGYRSPNLSRRKWFFFKEEDYDCSYFNKKGYYAFGRRSAKQSVDKQVKYLKKAVQ
                     KMRIN"
     gene            complement(1836..2540)
                     /locus_tag="ACUBV3_001910"
     CDS             complement(1836..2540)
                     /locus_tag="ACUBV3_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HHU3848380.1"
                     /translation="MRTVLVVQGDDETIELLRSYLEGALYKVVMASDGEEAFSLFQQH
                     QIDLAIIDITLPKIDGYELTRLIRQDSQIPIIMLAAKTTDMDRILGLNIGADDFITKP
                     FNSLEVLARINSQLRRYYEFNSLAKPKNQFIKIGELELDEEHVELTKNGKHIKLTATE
                     FKILHILMSSPGKIYTKTQLYEKINGRYLEGDETTIMVHISNIRDKIEDDSKYPKYIK
                     TLRGVGYKIEKPQYLV"
BASE COUNT          810 a          504 c          357 g          948 t
ORIGIN      
        1 atctctagaa cgttcaatga tttttccagt ttctgcatca atttgataat caacttcatt
       61 tttatttttc aggtccacaa agtcaatatc ataaactgat tttccatttt caatttcttc
      121 tttgatctta agattactga cttctgattc ctctatatta gcatctttaa atgcaatctt
      181 tttggcttct tcttgactta ttttatgaga tttttgactt gacgatgaac ttgactgaga
      241 agtcgatttt gaaaagccat tattatccat gtcccgctca aggatttgtc cagtcttagc
      301 atctacatga tagctgtatt ctgcgctttt ggtattgaat tcaatttcgt agctccctct
      361 aaagttttca aattctttgt caattttttg atgtatgaca tccttcttag ccactttagc
      421 atctttataa gcaatagcta acgcttgatt agttgtcttt tggaattgtg attggtaaaa
      481 taatacacca cctccaagga taacaacaag gactaaaaga actcctaaaa aaactttatt
      541 tttcttaatc atacagcacc ttctttctat tattcaattg tattttaaca taagctagac
      601 actttttcaa ttttgaccac tcgtttataa aaatatgtgg tcaataaaaa ccatttgaca
      661 ttaataccgc aaattaaaag ataccatagt atcatggtat cttttaatag tatatgtatc
      721 agttgatgcg cattttctgc acagccttct tcaagtattt gacttgttta tcaaccgatt
      781 gcttagcact acgccttcca aatgcgtaat atcctttttt attgaaataa ctacaatcgt
      841 aatcctcttc tttaaagaaa aaccatttcc ttctagatag atttggcgat ctataacctc
      901 tagtcatatc gtttcgaaat tttgggtcta actttacagg atcaacatct aattctttat
      961 gacgtgtatt atttttaaaa gcataattaa agctagcacc gttgataatg ccattacgtg
     1021 tgattacttc tgcgtcaatc tcgtttaaaa agtttccttt gtcatcaata ataaattccg
     1081 tatggaatga aattaaaagc ttactattaa taaccggctc atcatctggg aaaccaaaga
     1141 tttcaccatt tctccgacgt ttattatgca agcgagcaga ttcatagtag gcgatactat
     1201 ctggttctct taaacatgat aggtagttta ctaaagcatc tctatcagtc ataccaacat
     1261 ctttaaagta tcgtcgaaca tactgtgctt gctgactaga aatcaaatag cggaattgat
     1321 ggagtttcct atttaataca gtatcttcgg ctagactatt cccaggaaaa gaatcataaa
     1381 taacttgtga taaggctatc cagaaatcat catgcggagc taactcatct gaaaactgtg
     1441 cgaccaagta ttgtgtttct gataattctc ctgacaattc agttggcatt ttagcctgct
     1501 ctaacacgaa ttctaaagct ttgaggggtg cttgtaaact taaagaagtc caaaaatact
     1561 taaaaatagg agagccaact tttatatcat caatagcctt gagtcccttt aaaaagagta
     1621 atagatcatc tttaggtatg ctccatttat ctgctaaaga cagtatcatt tttatatttt
     1681 gataataatt ttctatccct tcaactgtaa aaggaatatt aagagcggtc tttgccatat
     1741 ctctcccctc caattttact tattatagca aaaaggaaag cttaaaacca agaaatatct
     1801 tcactaaaaa atggctctca ctagccatta tgtttttaaa caagatactg cggtttttcg
     1861 attttatacc ctactcctct aagtgttttt atatatttag gatatttact atcatcttcg
     1921 attttgtcac ggatattaga aatatgcacc ataatcgttg tctcatcacc ttctaaatac
     1981 ctaccattaa tcttctcata gagttgtgtt ttagtataaa tctttcctgg tgatgacatt
     2041 aagatatgaa gaatcttaaa ttctgtagca gtcaacttga tatgtttacc atttttagtt
     2101 agttcaacat gttcctcatc tagttccaat tcccctatct ttataaattg atttttaggt
     2161 ttggcaagac tattgaattc atagtaacga cgcagttgcg aattaattcg agctaaaacc
     2221 tccaaagaat taaacggttt cgtgataaaa tcatctgctc caatattcaa tcctaatatt
     2281 ctgtccatat ctgttgtctt agctgctagc ataataatag gaatctgact atcttgacga
     2341 atgagtcgcg tcaactcata tccatctatt ttaggtagcg taatatctat gattgctaaa
     2401 tcaatctgat gttgctgaaa taaagaaaaa gcctcctcac catcactagc catcacaacc
     2461 ttataaagag ctccctctaa atacgacctt aataactcta ttgtttcgtc atcaccttgc
     2521 actactaata ctgttcgcaa tgctccatac ccctttttat taatactatt ttcctttatg
     2581 tgtcaatcat attaattata ccaaaaaatc taattttgt
//
LOCUS       DBJYSH010000158         2590 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.158, whole genome shotgun sequence.
ACCESSION   DBJYSH010000158 DBJYSH010000000
VERSION     DBJYSH010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.158"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            30..>2590
                     /locus_tag="ACUBV3_001911"
     CDS             30..>2590
                     /locus_tag="ACUBV3_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GBS Bsp-like repeat-containing protein"
                     /protein_id="HHU3848381.1"
                     /translation="MRPIVRKKMYKKGKFLVIAGIVTILGGSAILGQDVKAEQAEAVT
                     STISEKTDSSQTISDISKLTLPVNSSEAMKNSAEPLIKTGFATSVSSNPREIAATPVK
                     TFDASSKVVVKASTAEHSANQTNSNVNQVANDSEVITQQNSTKQLPTVTYSAHVQDIG
                     WQKSVDNATVSGTVGQEKQVEAIKLSIKAPEGITGKLSYKTYVKGQGWQPSVESGQVS
                     GTVGQSRPIEALSINLTDNLQKLYDVYYRVHVQDIGWMAWAKNGAYAGTLGMSKRLEA
                     YKVKFTLKGQSVLTPTILKEERPVLNYQVKVGQNGWQSNKLEGQMAGTLGESKALDGV
                     KFTLSTLKYGDILYRTHVQDKGWGEILGSQTSKDYQGKRLEAIQLDLSGNLKQNYDIY
                     YRAHVQDKGWMAWQKNNSVAGTVGESKRLEALEVKLVAKASDFSSQTNHSFLEMKHPG
                     LSYQTYLQKDGWKPTVLEGQLGGSIGLSKSIKAIKLNLGSTALGNIEYRTFLNGSGWQ
                     TVVNSGRESNVPNESQQVEAIQMRLTGLLSKTYSVMYRVHMQDYGWQDWTYNNDIAGS
                     TNQGKRIEAIEIKLVETAKMPITVQTTYKGTGNYNVRVTNVISPGNLKIAIWSDKNNQ
                     DDLKWYTVTPKNHEARLTFNVTNHRDNGKYFIHVYQENASNQKQLLVKTSLQVAHSNY
                     NTPYFSQRDGRWASRRYGMATLGETGCVPTSLAMILSSLKGETVLPTQIADYLYHKTV
                     EFNRGVQGTTSRGILKAAKEWGVTATALGTQANLVQALKDGYHVLAAVQNNVFVLHGS
                     HEIVLKGYNNGLTHVSDPYTPSLSGWYPISQLWKEQSYYSEDRIDIGAPFVKVTDA"
BASE COUNT          915 a          414 c          537 g          724 t
ORIGIN      
        1 aaagaatatt aaaggtgagg ataaaatcta tgagacctat cgttagaaaa aagatgtaca
       61 aaaaaggtaa atttttggtc attgcaggta tcgttactat tttaggtggt agtgcaattc
      121 ttggtcaaga tgtcaaggca gaacaggcag aagcagtaac ttcaacgatc tcagagaaaa
      181 ctgatagcag tcagactatt tcggatattt ccaagttaac gttacctgtt aattcctcgg
      241 aggctatgaa aaatagtgca gagcctttaa taaaaacagg ctttgctaca tctgtttcat
      301 ctaatcctag agagatagca gctaccccag tcaaaacctt tgatgctagt tcaaaagttg
      361 ttgtaaaagc tagtactgct gagcactcag caaatcaaac aaattctaat gttaatcaag
      421 tagctaatga tagtgaagtt attactcaac aaaattcaac aaagcaatta ccaacagtga
      481 cttactctgc tcatgttcaa gatattggat ggcaaaagtc ggttgataat gcaacagttt
      541 caggaacagt aggacaggaa aagcaggttg aggcaataaa attgtctatt aaagcccctg
      601 aaggaataac aggaaaactt agctataaaa cttatgttaa aggacaaggt tggcaacctt
      661 cagtagagag tggtcaagtt agtggtactg ttggtcagag tagacctatt gaagcactaa
      721 gcattaacct tactgataat ttacaaaaat tatatgatgt ctattatcgt gttcatgttc
      781 aagatatcgg gtggatggct tgggctaaaa atggagctta cgctgggact ttgggaatgt
      841 caaagcgcct tgaagcttac aaagtaaagt ttacccttaa aggacaatct gttttaacac
      901 caactatact taaggaagag agacctgtct taaattatca ggttaaggta ggccaaaatg
      961 gttggcaatc taataaactt gaaggacaaa tggcgggaac tttaggagaa tccaaagccc
     1021 tagatggagt aaaattcact cttagcacct tgaagtatgg ggacattctc tatcgtactc
     1081 atgtacaaga taaaggttgg ggagaaattt taggtagtca aacaagtaag gattatcaag
     1141 gaaaaagact agaagctatt caattggact taagtgggaa tttaaaacaa aattatgata
     1201 tttattatcg cgctcacgtc caagataaag gttggatggc ttggcaaaaa aataatagtg
     1261 tagcaggtac agtcggagaa tcgaaacgtt tagaggcttt ggaagtaaaa ctagtagcta
     1321 aagcttcaga ttttagttcc caaacaaatc attcattctt agaaatgaag catcctggat
     1381 taagttatca aacttatctt caaaaagatg gttggaaacc aacggttttg gaaggccaat
     1441 taggaggtag tattggactt tcaaaatcta taaaagccat caaacttaac ttaggtagta
     1501 cagctctagg aaatatagaa tatagaactt ttcttaatgg ttcaggttgg caaactgttg
     1561 ttaattcagg gagagaaagt aatgtaccaa atgaatctca acaagtagaa gctattcaga
     1621 tgcgattgac aggtctgcta tcgaaaacct atagtgtgat gtatcgagtt cacatgcaag
     1681 attatggttg gcaggattgg acttataaca atgacatcgc tggtagtact aaccaaggaa
     1741 aacgaattga agcaatcgaa attaaattag ttgagactgc taaaatgcct ataactgtcc
     1801 aaacgaccta taagggaact ggaaactata atgtacgagt gacaaatgtt attagtcctg
     1861 ggaatttaaa aattgctatt tggtcagata aaaacaatca ggatgacctt aagtggtata
     1921 ccgtcacacc caaaaatcat gaagctagat tgacatttaa tgttacaaat catagagaca
     1981 atggtaaata ctttatccat gtttatcagg aaaatgcttc taatcaaaaa caattattgg
     2041 taaaaacttc tctacaagtc gcacattcaa attataatac tccttacttt agccaacgtg
     2101 atggaaggtg ggcaagtcga agatatggta tggcaacttt aggagaaact ggttgtgtac
     2161 caacttctct agctatgatt ttgtcttctt tgaaaggaga aactgtttta ccaacgcaaa
     2221 ttgcagatta cctttaccat aagacagttg aatttaatcg tggtgttcaa ggaacaactt
     2281 ctagaggaat cttaaaagca gccaaagaat ggggtgtaac agcaactgca ttaggaactc
     2341 aagctaacct tgtacaagcg ttgaaggatg gttatcatgt cctagcggca gtacaaaata
     2401 atgtttttgt tcttcatgga agtcatgaaa tcgttctcaa aggatataat aacggactga
     2461 cacatgtctc tgacccttac acaccgtcct tgtctggttg gtatccaatt tcacaacttt
     2521 ggaaagaaca gagctattat agtgaggata gaattgatat tggagcacct tttgtcaagg
     2581 taacagatgc
//
LOCUS       DBJYSH010000159         2564 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.159, whole genome shotgun sequence.
ACCESSION   DBJYSH010000159 DBJYSH010000000
VERSION     DBJYSH010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2564
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.159"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            137..889
                     /locus_tag="ACUBV3_001912"
     CDS             137..889
                     /locus_tag="ACUBV3_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HHU3848382.1"
                     /translation="MNIFILEDDFVQQAHFEKIIKEIRVQYNLHFKTVETFAKPVQLL
                     ESIYEIGLHNLFFLDIEIKNDEQMGLEVAKQIRQVDPYAQIVFVTTHSELMPLTFRYQ
                     VSALDYIDKGLSQEEFSQRIEEVLLYVDGICNKPLVENSFYFKSRYSQVQLPFNDLLY
                     IETSSRSHRVVLYTEKDRMEFTATLGDILKQEPRLFQCHRSFLVNPLNIFKVDRIDRL
                     VYFQNGTTCLVSRNKVRDIVSIVDKYQKDRKR"
     gene            958..1257
                     /locus_tag="ACUBV3_001913"
     CDS             958..1257
                     /locus_tag="ACUBV3_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HHU3848383.1"
                     /translation="MANMIDYSHHIEVSLETDEPKIAMSYFTLNHKQIFIIENSIVEE
                     RIDASRLFGYGASSKGEDRGLGLYNVMKVIDRYPNASISTNSEQHTFRQGLEIIF"
     gene            1380..2228
                     /locus_tag="ACUBV3_001914"
     CDS             1380..2228
                     /locus_tag="ACUBV3_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000926579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HHU3848384.1"
                     /translation="MLLQLDNITKSYGKKIVLNQISYQFTPGLYGLLGANGTGKTTLL
                     NLMSHFTFADSGNIYWNGQEQSEEFYRHIGFLPQHFRYYDQFTGIAFLNYIATLKGVD
                     KKKAKQEIPRLLELVGLGDVGKKKISSYSGGMKQRLGIAQALINDPEILILDEPTVGL
                     DPKERVKFRHILSQLSTNKIIILSTHIVSDVEAVAKEIIVLKNGKFIEHGNTAQLLKT
                     IEGKVWEITTEPGLSQIPNIAIVNEKVFSDSRVFRVVSDICPSDSAQLVVPTLEDFYI
                     YHFRGE"
     gene            2234..>2564
                     /locus_tag="ACUBV3_001915"
     CDS             2234..>2564
                     /locus_tag="ACUBV3_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3848385.1"
                     /translation="MISIFPFELKKLLQRKAAFGAFIIFLLAIFGLFYQHFFVGQISG
                     YSADKIHGREAVAINRRIAEKYSGDLSEERVTNIIKNYVNPPAGRPPIFDVFSYYVAD
                     TFDRDFLI"
BASE COUNT          853 a          384 c          479 g          848 t
ORIGIN      
        1 tattatgctt ttaaaattag cctcatggac gtcacatatt aagacgggat aactcataaa
       61 cgatacctat gagaagaata tcgtagtggc tctattgtga ctattaatat tcaagtaata
      121 agatagagga tataaggtga atatttttat acttgaggat gattttgttc aacaagccca
      181 ttttgaaaaa atcattaaag agattagagt acagtacaat ctgcatttca aaacagtaga
      241 aacgtttgct aaaccagttc aattattgga atcaatttat gaaataggtt tacataattt
      301 atttttctta gatattgaaa taaaaaatga tgagcagatg ggcttagagg tagcaaaaca
      361 aattagacaa gttgatccat atgctcagat tgtttttgtt acaactcatt cagaattaat
      421 gcctttaacc tttcgttatc aggtatcagc tttagactat atcgataaag gactttcaca
      481 ggaagagttt agccaaagga ttgaggaagt attgttgtac gtagatggca tttgcaataa
      541 gcccttagtg gaaaattcat tctactttaa gtcgcgttac agccaggtac aattaccttt
      601 taacgatctt ctttatatag aaacatcctc aagatcacat agagttgtcc tttatactga
      661 aaaagataga atggaattca cagccacttt gggggatatt ttaaaacaag aacctaggct
      721 ctttcaatgt catcgctctt ttttagtaaa tcccttaaat atttttaaag tggataggat
      781 agatagatta gtttacttcc aaaatggaac gacttgtctg gtttcacgca ataaagtgag
      841 agatatagta tctatagtgg ataaatatca aaaggataga aaaagatgag ttctatttta
      901 gaccgttact tcaaaacttc tttacaggag aggttggttg ttcaaagaaa actctaattg
      961 gctaatatga tagattatag ccaccatatt gaagtttctt tagagacaga tgaacctaag
     1021 atagccatgt cttattttac attaaaccat aagcaaattt ttatcataga aaattcgata
     1081 gtcgaggaaa gaatagatgc ttcacgtttg tttggttatg gagcaagtag caagggagaa
     1141 gatagagggt taggccttta taatgtcatg aaagttattg accgttatcc caatgcatct
     1201 attagtacaa atagtgaaca acatactttc cgtcagggct tagaaataat cttttagcta
     1261 tattacttta caatcattta gagagtaagt gagaaagctc atttttaacc tttagctcag
     1321 tcgctatatt ttagtaattg atttttcaac tgtcttaatt attgaaagga gacaatacta
     1381 tgttattaca gctagataat atcactaaaa gctacggaaa aaaaatagta cttaaccaga
     1441 tttcctacca gtttactcct ggtttgtatg gactcttagg agcaaatggt actggcaaaa
     1501 caaccttatt aaatttgatg agccacttta cattcgcaga tagtgggaat atttattgga
     1561 atggacagga gcagtcggaa gaattttatc gtcacattgg ttttttaccg caacattttc
     1621 gctattatga tcaatttact ggcattgctt tcttaaatta tatcgctacc ttaaaaggtg
     1681 ttgataaaaa gaaagctaaa caagaaattc ctagattact agagcttgtt ggtttaggcg
     1741 atgtaggtaa aaagaagatt tcatcatact ccggtggtat gaagcaacgt ttaggcattg
     1801 ctcaagcact tatcaatgat cctgaaatac tcattctgga tgaaccaact gttggacttg
     1861 atcccaagga acgggttaaa tttcgccata ttttgagtca gttatccacc aataaaatta
     1921 tcattttatc aacgcatatt gtgtcggatg tggaagctgt tgccaaagaa attatagtat
     1981 taaaaaatgg taaatttatc gaacatggca acaccgcaca gctgttgaaa actattgagg
     2041 gaaaagtgtg ggaaataacg actgaaccag gtttatctca gatacccaat attgctattg
     2101 ttaatgaaaa agtcttttca gatagtcggg tatttcgtgt agtgagtgat atttgtccga
     2161 gtgattcggc acagttggtt gttccgactc tagaagattt ctacatctat cattttagag
     2221 gggagtaagt aagatgatat caatttttcc ttttgaatta aaaaaacttc tacaacgtaa
     2281 ggcagcattt ggggctttta ttatcttcct tcttgctatt tttggtctat tttaccagca
     2341 tttttttgtt gggcaaatta gtggctattc tgcagataaa atacatggtc gagaagcggt
     2401 tgccattaat cgtagaatag ctgaaaaata ttcaggggat ttatctgaag aacgagtaac
     2461 aaatattatt aaaaactatg ttaatccgcc agcaggacgc ccccctatat ttgatgtttt
     2521 tagttattat gtggcagaca cttttgatcg tgatttttta attt
//
LOCUS       DBJYSH010000160         2506 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.160, whole genome shotgun sequence.
ACCESSION   DBJYSH010000160 DBJYSH010000000
VERSION     DBJYSH010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2506
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.160"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            complement(18..503)
                     /locus_tag="ACUBV3_001916"
     CDS             complement(18..503)
                     /locus_tag="ACUBV3_001916"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000409123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HHU3848386.1"
                     /translation="MEIWDAYDQYGFITGLTLNRDQNIPQGLFHLVVDVILFHEDGDV
                     LMMKRHPKKKAFPAYFEATAGGSALKGENAKQAILRELKEETGIVPQCLTFLNREWFS
                     ERSYFVDHFIAKYNGAKDIITLQEGETVDYIWLKPEYIDLFLSKNKLIPSQIKLLKSL
                     I"
     gene            complement(516..971)
                     /locus_tag="ACUBV3_001917"
     CDS             complement(516..971)
                     /locus_tag="ACUBV3_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HHU3848387.1"
                     /translation="MTELDNKILQKASGENRLDPDQQRYYLGTYKERVVLHLSLKEAQ
                     EEKFQLAITSALNNLILEYPNLSLKLSPNLPTDVQMFYMKLANRLSLAATIVNEANAN
                     SPFGIIIHSDKAENVEWNDFETQFPDLFNSPKKEESPKKSLWQHFFSQK"
     gene            1169..2476
                     /gene="eno"
                     /locus_tag="ACUBV3_001918"
     CDS             1169..2476
                     /gene="eno"
                     /locus_tag="ACUBV3_001918"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-displayed alpha-enolase"
                     /protein_id="HHU3848388.1"
                     /translation="MSIITDVYAREVLDSRGNPTLEVEVYTESGAFGRGMVPSGASTG
                     EHEAVELRDGDKSRYSGLGTQKAVDNVNNVIAEAIIGYDVRDQQAIDRAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYSYLGGFNTKVLPTPMMNIINGGSHSDAPI
                     AFQEFMIMPVGAPTFKEALRWGAEVFHALKKILKERGLETAVGDEGGFAPKFEGTEDG
                     VETILKAIEAAGYEAGENGIMIGFDCASSEFYDAERKVYDYSKFEGEGGAVRTAAEQI
                     DYLEELVNKYPIITIEDGMDENDWDGWKALTERLGGRVQLVGDDFFVTNTDYLARGIK
                     EEAANSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTIADIAVATNAG
                     QIKTGSLSRTDRIAKYNQLLRIEDQLGEVAQYKGIKSFYNLKK"
BASE COUNT          785 a          442 c          492 g          787 t
ORIGIN      
        1 tttattaagc ctagttttta gattaaggat tttaagagtt ttatttgaga gggtattaat
       61 ttgtttttag ataaaaataa atcaatatat tctggtttta accagatata gtcaacggtc
      121 tctccttctt gcaatgtaat tatatctttt gcaccattgt attttgcgat aaaatggtct
      181 acaaagtaag aacgctcaga gaaccactct ctattaagaa aggttagaca ttgaggaacg
      241 ataccagttt cttctttgag ttcccttaga atagcttgct tagcattttc tcctttaagg
      301 gcagatccgc cagcggtggc ttcgaaataa gcaggaaaag ctttcttttt agggtgccgt
      361 ttcatcatca atacgtcacc atcctcatga aaaaggataa catcaacaac taagtggaat
      421 aagccttgtg ggatattttg atccctatta agtgtaagac ctgtaatgaa cccatattgg
      481 tcataagcat cccaaatttc cattagacct ccttgttatt tttgactaaa gaaatgttgc
      541 catagacttt ttttaggaga ctcctctttt tttggtgaat tgaataaatc agggaattgt
      601 gtctcaaaat cgttccattc aacgttttca gccttatcac tatgtataat aataccaaat
      661 ggagaattgg catttgcttc attaacaatc gtagcagcta gtgatagacg gttagctaac
      721 ttcatgtaga acatttgaac atcagtaggg agatttggtg ataattttaa actaagatta
      781 ggatattcaa gtatcagatt attaagagca gatgttatag caagttgaaa cttttcttct
      841 tgtgcctctt ttaaacttaa gtggagcaca actctttctt tataagtacc aaggtagtaa
      901 cgttgttgat caggatcaag acgattttca ccgcttgctt tttgtaggat tttattgtca
      961 agttcagtca taatttctcc ttgaatttgc taattttatt ataacctaaa tgggttgcat
     1021 tagcacaggt aaacttgaaa agaattagtg atttttttat caaaaaccac gtaataaagc
     1081 ggtttcactg ggtttttcct tgttattaac taaagattat gatattatga atatgtaaaa
     1141 aattaaaact cataaggaga gtattgtaat gtcaattatt actgatgttt acgcacgcga
     1201 agtccttgac tcacgcggta acccaacact tgaagtagaa gtttatacag aatcaggtgc
     1261 tttcggacgt ggtatggttc cttcaggagc ttctactggt gaacacgaag cagttgaact
     1321 tcgcgacggc gacaagtctc gttacagtgg tcttggaaca caaaaagctg ttgacaatgt
     1381 aaataacgtt attgcagaag caatcattgg ttatgatgta cgcgaccaac aagctatcga
     1441 ccgtgctatg atcgctcttg acggaactcc taataaaggt aaattaggtg caaatgctat
     1501 tcttggtgtt tctattgccg tagcacgtgc tgcagctgat taccttgaag taccacttta
     1561 cagctacctt ggtggattca acactaaagt tcttccaact ccaatgatga acatcatcaa
     1621 cggtggttct cactcagacg ctccaatcgc attccaagaa ttcatgatta tgccagttgg
     1681 tgcaccaact ttcaaagaag cacttcgttg gggtgctgaa gtattccatg ctcttaagaa
     1741 aatccttaaa gaacgtggtc ttgaaacagc tgttggtgat gaaggtggat ttgcacctaa
     1801 atttgaaggt actgaagatg gcgtagaaac tattcttaaa gctattgaag ctgctggtta
     1861 cgaagctggc gaaaacggaa tcatgattgg ttttgactgt gcttcatcag agttctacga
     1921 tgcagaacgt aaagtatacg actacagtaa attcgaaggt gaaggtggag cagttcgtac
     1981 tgctgcagaa caaatcgatt accttgaaga gttggttaac aaatacccaa tcatcactat
     2041 tgaagatggt atggatgaaa acgactggga tggttggaaa gctcttacag aacgccttgg
     2101 tggacgtgta caattagttg gtgacgattt cttcgttaca aatactgatt acttagcacg
     2161 tggtatcaaa gaagaagctg ctaactcaat ccttatcaaa gttaaccaaa tcggtacttt
     2221 gacagaaact ttcgaagcta ttgaaatggc taaagaagct ggttacactg cagttgtatc
     2281 acaccgttca ggtgaaactg aagattcaac aatcgctgat atcgcagttg caactaacgc
     2341 tggtcaaatt aaaacaggtt cattatcacg tactgaccgt attgctaaat acaaccaatt
     2401 acttcgtatc gaagaccaac ttggtgaagt tgctcaatac aaaggtatca aatcattcta
     2461 caacttaaaa aaatagtctc agattattaa acagttgaaa atataa
//
LOCUS       DBJYSH010000161         2465 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.161, whole genome shotgun sequence.
ACCESSION   DBJYSH010000161 DBJYSH010000000
VERSION     DBJYSH010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2465
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.161"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            complement(88..768)
                     /locus_tag="ACUBV3_001919"
     CDS             complement(88..768)
                     /locus_tag="ACUBV3_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HHU3848389.1"
                     /translation="MKEAKNFSQQLNILRAGVLGANDGIISVAGVVIGVASATHNLWI
                     IFLSAASAILAGAFSMAGGEYVSVSTQKDTEQAAVAREEKLLENNPELAKKSLVDIYL
                     AKGESHEHAQWLVDKAFSKNAIEHLVEEKYGIEFGEYTSPWHAAISSFIAFAIGSIFP
                     TITILLLPFSVRIVGTVIIVIVSLLSTGYVSAKLGQAPTVPAMRRNVMIGCLTMLATY
                     VIGQLFSI"
     gene            921..1949
                     /gene="queA"
                     /locus_tag="ACUBV3_001920"
     CDS             921..1949
                     /gene="queA"
                     /locus_tag="ACUBV3_001920"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HHU3848390.1"
                     /translation="MNTNDFDFYLPEELIAQTPLEKRDASKLLVIDHKNKTMTDSHFD
                     HILDELKPGDALVMNNTRVLPARLYGEKPDTHGHVELLLLKNTEGDQWEVLAKPAKRL
                     RVGNRVSFGDGRLIATVTKELEHGGRIVEFSYDGIFLEVLESLGEMPLPPYIHEKLED
                     RDRYQTVYAKENGSAAAPTAGLHFTKELLEKIETKGVKLVYLTLHVGLGTFRPVSVDN
                     LDEHEMHSEFYQLSKEAADTLNAVKESGGRIVAVGTTSIRTLETIGSKFNGELKADSG
                     WTNIFIKPGYQFKVVDAFSTNFHLPKSTLVMLVSAFAGHDFVLEAYNHAVEERYRFFS
                     FGDAMFVK"
     gene            2036..>2465
                     /locus_tag="ACUBV3_001921"
     CDS             2036..>2465
                     /locus_tag="ACUBV3_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HHU3848391.1"
                     /translation="MSNRSILDERLCFTVSETHRLFNKFYQKALDEFQLTYLQYVILL
                     VLWEEDHRHMKELSYALSLNSNTLTPVLKRMENRGWIVREKPRDDKRQLIIHLSNKGI
                     ESQDAVVNAVQSCGYFFSEISPESYQKTVSALSSLNQVIKS"
BASE COUNT          807 a          454 c          436 g          768 t
ORIGIN      
        1 tgtcaataaa aacttcttaa tttcgttata aaactaaaaa gaattactgt ccattctagt
       61 ttagacaata actccacgat taatatttta tattgaaaat aattgtccaa tgacataggt
      121 tgccaacatt gtcaaacaac caatcatcac atttcgacgc atggcaggta ctgtaggggc
      181 ttggcctaac ttagcactta cataaccagt tgataatagt gaaacaataa caattattac
      241 cgttcctaca attcgtaccg aaaatggtaa taacagaata gttatagttg gaaaaattga
      301 tccgatggca aaagctataa aactagatat agcagcatgc caggggcttg tatattcacc
      361 gaactcaata ccatattttt cttcaactaa atgctcaata gcattctttg aaaaagcctt
      421 gtcaactagc cattgagcat gttcgtgact ttctcctttt gctaagtaga tatcaactaa
      481 ggattttttg gcaagttctg gattattttc tagtagtttc tcttcacgcg caactgctgc
      541 ttgttcagta tctttttgag tagaaacact aacatactcc ccgccagcca tagaaaatgc
      601 accagctaaa atcgcggaag ctgctgataa aaaaataatc caaaggttat gagttgcact
      661 cgcaacacca ataacaacac cagctacaga gatgatacca tcattagcac caagaacacc
      721 agctcttaaa atattcaatt gttgggaaaa attttttgct tctttcatct tctctacttc
      781 tcactttctg acaattatta taccacttat ttagaatgat tacaattatt ttttataatc
      841 attctaaata agaagattag ttgggtcatt tttaccgaac gtgatataat agaaaagctt
      901 acttatagat aggagaaatc atgaatacaa acgattttga tttttattta ccagaagaac
      961 ttattgccca aacaccactt gaaaaaagag acgcttctaa actattagtc attgatcata
     1021 agaataagac gatgacagat agccatttcg atcatatctt agatgaactc aagcctggtg
     1081 acgctttagt aatgaataac acacgtgttc tgcccgctcg cctttatggt gaaaagccag
     1141 atacacacgg ccatgtagag ttattacttc tcaaaaatac agagggcgat caatgggaag
     1201 tgcttgctaa acccgctaaa cgcttacgcg tagggaacag agtttcattt ggtgatggtc
     1261 ggttaatagc aacagtcaca aaggaattag aacatggtgg gcgtattgta gaattctcat
     1321 atgatggtat ttttctagaa gtccttgaaa gtttgggcga aatgccactt ccgccatata
     1381 ttcatgaaaa actagaagat cgtgaccgct atcagactgt gtatgcaaaa gaaaatggtt
     1441 ctgctgctgc accaacagcc ggtcttcatt ttacaaaaga actattggaa aaaattgaaa
     1501 caaaaggtgt gaagcttgtc tatttgacgc ttcacgttgg acttggaact ttccgtcctg
     1561 tttcagttga taatcttgat gaacacgaaa tgcattctga gttttaccaa ttatctaaag
     1621 aagctgctga tactctcaat gcagttaaag aatctggtgg acgtattgta gctgttggta
     1681 caacttctat tcgcacatta gagacaattg gttctaaatt caatggtgaa ttaaaagccg
     1741 actcagggtg gacaaatatt tttatcaagc ctggttatca gttcaaggtt gtagacgctt
     1801 tttcaaccaa ctttcactta ccaaagtcta ctcttgtcat gttagtatca gcatttgctg
     1861 gacatgactt tgtactagaa gcttacaacc atgcagttga agaacgatat cgtttcttca
     1921 gttttggtga tgctatgttt gttaagtaag aataggcttt caattaggaa aaagttttat
     1981 cgttttttaa cacgattgac atactataac gcctccaaaa tgctacaata tgataatgag
     2041 taatcgaagt attttagacg agcgattatg ttttacggtt agtgaaacac atcgactttt
     2101 caataaattt taccaaaaag ctttggatga atttcaattg acttatctcc aatacgttat
     2161 cttgttagtc ctttgggaag aggatcatcg gcatatgaag gaacttagtt atgcactatc
     2221 tcttaattcg aataccctaa ctccagtttt aaagcgtatg gaaaatcgtg ggtggattgt
     2281 acgagaaaaa ccaagagatg acaaaaggca actcattatt catctaagta acaaaggtat
     2341 tgagtcacaa gatgccgttg tcaatgcggt gcaatcgtgc gggtattttt tctcagaaat
     2401 tagtcctgaa tcttatcaaa aaactgtttc tgccctttct tcacttaatc aagttataaa
     2461 atcac
//
LOCUS       DBJYSH010000162         2393 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.162, whole genome shotgun sequence.
ACCESSION   DBJYSH010000162 DBJYSH010000000
VERSION     DBJYSH010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.162"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            complement(157..1353)
                     /locus_tag="ACUBV3_001922"
     CDS             complement(157..1353)
                     /locus_tag="ACUBV3_001922"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HHU3848392.1"
                     /translation="MAKLTVKDVDLKGKKVLVRVDFNVPLKDGVITNDNRITAALPTI
                     KYIIEQGGRAILFSHLGRVKEEADKEGKSLAPVAADLAAKLGQDVVFPGVTRGAKLEE
                     AINALEDGQVLLVENTRFEDVDGKKESKNDEELGKYWASLGDGIFVNDAFGTAHRAHA
                     SNVGISANVEKAVAGFLLENEIAYIQEAVETPERPFVAILGGSKVSDKIGVIENLLEK
                     ADKVLIGGGMTYTFYKAQGIEIGNSLVEEDKLDVAKDLLEKSNGKLILPVDSKEANAF
                     AGYTEVRDTEGEAVSEGFLGLDIGPKSIAKFDEALTGAKTVVWNGPMGVFENPDFQAG
                     TIGVMDAIVKQLGVKSIIGGGDSAAAAINLGRADKFSWISTGGGASMELLEGKVLPGL
                     AALTEK"
     gene            complement(1488..2357)
                     /locus_tag="ACUBV3_001923"
     CDS             complement(1488..2357)
                     /locus_tag="ACUBV3_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase, lipoprotein e(P4) family"
                     /protein_id="HHU3848393.1"
                     /translation="MKTTKVFSVLSLSVAAFMMTACQSNHSTKKADMSPDKVEQKTKF
                     KNISLSSNKLLAKENTMSVLWYQNSAEAKALYLQGYNVAKMKLDDWLQKPSEKPYSII
                     LDLDETVLDNSPYQAKNIKDGSSFTPESWDKWVQKKSAKAVAGAKAFLKYANEKGIKI
                     YYVSDRTDAQVDATKENLEKEGIPVQGKDHLLFLKKGMKSKESRRQAVQKDTNLIMLF
                     GDNLVDFADFSKSSSTDREQLLTKLQSEFGSKFIVFPNPMYGSWESAIYQGKHLDVQK
                     QLKERQKMLHSYD"
BASE COUNT          726 a          488 c          376 g          803 t
ORIGIN      
        1 gtcataaact tttgaaaatt gcagtgtcaa ccaaatacca ctatatctaa aaactagtaa
       61 atgataagtg aagtcaagga aaattcaata actctacatt atcaactaaa aaaagacatg
      121 catcagcatg tcctttaata taaattataa tgactattat ttttcagtca atgctgccaa
      181 acctggtaat actttacctt cgagcaattc catgcttgct ccaccaccag tagagatcca
      241 tgagaatttg tcagcacggc caaggttgat agcagctgct gctgaatcac caccaccgat
      301 gattgattta acgcctagtt gtttaacgat agcgtccatt acaccgattg taccagcttg
      361 gaagtcaggg ttttcaaaga cacccatagg tccgttccat acaactgttt tagcaccagt
      421 aagtgcttca tcaaatttag cgattgattt aggaccgatg tcaagaccaa ggaacccttc
      481 tgaaactgct tcaccttcag tgtcgcgaac ttcagtataa ccagcaaatg cgtttgcttc
      541 ttttgagtca actggcaaga tcaatttacc gtttgatttt tcaaggaggt ctttagcaac
      601 atccaatttg tcttcttcta caagtgagtt accgatttcg ataccttgag ctttgtagaa
      661 tgtgtaagtc ataccaccac cgataagaac tttatcagct ttttcaagaa ggttttcgat
      721 aacaccaatc ttatcagaaa cttttgagcc accaagaata gctacgaatg ggcgttctgg
      781 agtttcaact gcttcttgga tgtaagcaat ttcgttttca agaaggaaac cagctacagc
      841 tttttcaacg tttgctgaaa tacctacgtt tgatgcatga gcacggtgtg ctgtaccaaa
      901 tgcatcgtta acgaagattc catctccaag tgaagcccag tatttaccaa gttcttcgtc
      961 attcttagat tctttcttac cgtcaacatc ttcaaaacga gtgttttcaa ccaaaagaac
     1021 ttgtccatct tccaaagcat tgattgcttc ttctaatttt gcaccacgag taacacctgg
     1081 gaatacaaca tcttgaccaa gtttagcagc taaatcagca gctaccggtg caagtgattt
     1141 tccttctttg tcagcttctt ctttaacacg tccaaggtga gagaagagga tagcacgacc
     1201 accttgttcg atgatatact tgattgttgg aagagccgca gtgatacggt tgtcgttagt
     1261 gataacgccg tctttcaaag gcacattaaa gtcaacacga acgaggactt ttttaccttt
     1321 caaatcaacg tctttaacag tcaatttagc cattagtaaa actccttata taatttgata
     1381 ttatttatta taacatattt ttgtgaacag ttgtaattat tgattacttt ttcacaaata
     1441 gatttcatag attttcctag ttgttttcaa gagaattcgt atacttatta atcatacgaa
     1501 tgcaacattt tttgtcgttc tttcaattgt ttttgaacat ccagatgttt tccttgataa
     1561 atagcacttt cccaagaacc gtacatagga tttgggaaaa caataaattt actaccaaac
     1621 tcactttgaa gtttagttag tagttgttct ctatctgtac tagatgattt agaaaaatca
     1681 gcaaaatcaa ctaaattatc tccaaaaagc ataattaaat tagtatcttt ttgaactgcc
     1741 tgacggcgac tctctttaga tttcattcct tttttaagga aaagcaagtg gtctttccct
     1801 tgaacaggta taccttcctt ctctaaattt tctttagtcg catcaacttg agcatctgta
     1861 cgatctgaga cataataaat ttttattccc ttttcattag catacttcaa aaatgctttg
     1921 gcacccgcaa cagccttagc tgacttcttt tgcacccatt tatcccaact ctctggcgtg
     1981 aaactagagc catctttaat attctttgct tgatatgggc tattatctaa aactgtttca
     2041 tctaaatcta agataattga atatggtttt tcactgggct tttgtaacca atcatctaac
     2101 ttcattttag caacattata accttgtaaa tataaagcct tggcttctgc agaattttga
     2161 taccaaagga cagacatagt gttttcctta gccaacagtt tattagaact tagagaaata
     2221 tttttaaatt tcgtcttttg ctccacctta tctggagaca tatcagcttt tttagtgctg
     2281 tgatttgact gacatgctgt catcataaat gcagcaacag ataacgataa aactgaaaaa
     2341 acttttgtag tcttcataaa acttctcctc tatatttttt caattaaatc ata
//
LOCUS       DBJYSH010000163         2361 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.163, whole genome shotgun sequence.
ACCESSION   DBJYSH010000163 DBJYSH010000000
VERSION     DBJYSH010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2361
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.163"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            221..697
                     /locus_tag="ACUBV3_001924"
     CDS             221..697
                     /locus_tag="ACUBV3_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001869194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3848394.1"
                     /translation="MYIIPAGYSPERLDSEVLTAKIEDDEALLRKIQDLKDHLDKNTK
                     ILAYAFDGLEVRVKARLRKNRKKLKNLIEFNAKSTSVFNELKDYSNAVSSGLTFVSKG
                     FSSFNGQFSSASNLDWQKTIKTGMRKREEDAKQLRKKALEDSNKLRKIASKRGYFE"
     gene            953..1290
                     /locus_tag="ACUBV3_001925"
                     /pseudo
     CDS             953..1290
                     /locus_tag="ACUBV3_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285002.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1684..2064
                     /locus_tag="ACUBV3_001926"
     CDS             1684..2064
                     /locus_tag="ACUBV3_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3848395.1"
                     /translation="MVFVSDGSPVDANTLYFGKGTPIFMIMNVTIFIISTLIYSYYYL
                     PKNQGKVWKFNQWNNEKSREQQYISNFIVTFGLILLVTPFLKYGIYNSFGVLISLLFT
                     ATLPSLIVDSLYAAFYVHKHPEYE"
BASE COUNT          858 a          300 c          429 g          774 t
ORIGIN      
        1 aatatattgc attgaattgg ttgtgtgcga tagccacgct atactattaa gttattacaa
       61 actatgaatt acacctcaaa tgttggagat aaaacctatc gtttggggtg ttttttggta
      121 aaatagaatg aaaagagtta ctctgtgtca ttactaagtt aaaaaattat actaacttaa
      181 ttattaacgt tttattgact gagttactct tttttaaaaa atgtatatca ttccagctgg
      241 ttactcacct gaacgtttag actcagaagt ccttacagca aagattgaag atgatgaagc
      301 cttattgcgg aaaattcaag accttaaaga tcatttagat aaaaatacca aaatcttggc
      361 ttacgctttt gatggcttgg aagtcagagt gaaagctaga ctaagaaaaa atcgtaagaa
      421 actcaaaaac cttatagaat tcaatgccaa atctacctca gtctttaatg aattgaagga
      481 ttattcaaat gctgtatcgt ctggtttaac ctttgtatct aaaggcttta gtagtttcaa
      541 tggccagttc tcttcagcct caaatcttga ttggcaaaag actattaaga caggaatgag
      601 aaaacgagaa gaagatgcta aacagttgag aaaaaaagct ttagaagatt caaataagct
      661 cagaaaaatt gcatcaaaaa ggggttactt tgaataatgc agttgaattt gctaaagaac
      721 agaacaaacc cctaaaaata aaaagataac catagataaa tatggtcgtg taaaaatgga
      781 taaaactttt ctttatcgaa aagctaaaga tggaaaatct gcatatattt attctgaagg
      841 gagtgatcaa tataaagaac taactgggaa agaatttgat cctaaaacta caaatattat
      901 gaaaggtaga aaattaggga cagatggtaa gatatcaatt gattttggtg agattagtaa
      961 ctcaggaaaa aatgcagcta ttaagtccct taaaaatgaa agtccttgaa ttaaatccca
     1021 aaaatgcttt taaaggatgg aatgagttaa gcaaaatagc taaaactggg aaagtactag
     1081 gatggacagg taaaattgct aatatttaca tgaatggaaa aaaagatctt tttgatgata
     1141 aaacatcttc aatagatcaa aaaatcaaaa actttgctat aggttcaggt gtagatgcat
     1201 tgtccggtgc agcaataggg actttggtag gaggacctgt gggaaccgtt gttggagcag
     1261 gtgcagggct ggcatttgat tttgctttga attttaaagt taaggagttg ggtaatgaat
     1321 caattacagg ttttgctaaa aaaggtttaa aaaatcttgc aggaggatta ggattttgaa
     1381 aaaaagtata aatgtaagta ggtatcatgg acctttaatt gaatttgagg ttgatgcaag
     1441 ttatttaaga attttttcat atatcggttt acctttatca ttgttttggt attttgttac
     1501 tcataacagt gatgactttg gaatattaaa atcgatattt ttaatttgta ttatagtcaa
     1561 tttaatcggg ctcatattag gattaataca aaagtttact aaaaaactta tcaaatctct
     1621 atcatttttt gtactttctt agcgtttctt tcgattttct tagggttaga tacaatatgt
     1681 ttaatggtat ttgtttcgga tgggtcccct gttgatgcga atacgttgta ttttggtaag
     1741 ggtactccaa tttttatgat aatgaacgtt acgatattta tcatttctac tttgatctat
     1801 tcctactatt atttaccaaa aaatcaaggg aaagtttgga aatttaatca atggaataac
     1861 gaaaagagtc gagaacaaca atacataagt aattttattg ttacatttgg tctgatattg
     1921 ttagttactc catttttaaa gtatggtatc tataacagtt ttggagtttt aatcagttta
     1981 ctatttacag caaccttacc atcattaatt gttgattcac tttatgcggc attttatgtc
     2041 cataagcatc ctgagtatga ataatcaact tataaagaac atcgaaaaca catgatacct
     2101 tttataagac agaaactata aattcagata aaacatcttc gcttgataag tcagagactt
     2161 taaagataga ggataacaag ctattttaga caaaaaatta gaaatttcac aatagattct
     2221 agagtagata ctttattagg tgctggaagg ttattgggca cttgattgtg attggactgg
     2281 acaaaacaaa ccagatcaat cagtaactgg ttttgcaaaa aagaattaaa agatttattt
     2341 ggagggtaaa atttttgaaa c
//
LOCUS       DBJYSH010000164         2353 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.164, whole genome shotgun sequence.
ACCESSION   DBJYSH010000164 DBJYSH010000000
VERSION     DBJYSH010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2353
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.164"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            35..1117
                     /locus_tag="ACUBV3_001927"
     CDS             35..1117
                     /locus_tag="ACUBV3_001927"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HHU3848396.1"
                     /translation="MITKFPDQWQDKLTQRQFDDLTDIQNKLFQPITDGDNILGISPT
                     GTGKTLAYLFPTLLKLQPKKSQQLLILAPNSELAGQIFDVTKEWAEPLGLTAQLFLSG
                     SSQKRQIERLKKGPEILIGTAGRVFELVKLKKIKMMNINTIVLDEFDELLGDSQYHFV
                     DNIINRVPRDQQMIYISATNKLDNSKLADNTITIDLSDQKLDTIKHYYITVDKRERTD
                     LLRKFSNIPDFRGLVFFNSLSDLGACEERLQFNRASAVSLASDINIKFRKVILEKFKN
                     HDISLLLGTDLVARGIDIDNLEYVINFDIARDKETYTHRSGRTGRMGKEGCVITFVTH
                     KEELKQLKKYATVAELVLHNQKLHLK"
     gene            complement(1149..>2353)
                     /locus_tag="ACUBV3_001928"
     CDS             complement(1149..>2353)
                     /locus_tag="ACUBV3_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HHU3848397.1"
                     /translation="AWQGFSFLKHKEIKLQQAVVEKEIRIAEKTVEVVKRQKTERVLF
                     LEPKGYDKSLSADILKWNQKSFEHKKFYDNQYIILRPQLADSNFANVKKLSIYQILYQ
                     KEKGSMFQKSSRLLRTYLLDQNKKPFELDELLAHNISGFKAILENIAPGTQLKEHDSN
                     KEFLKTGRVTDGLDVKDGKLIINDLKLPLDKLYNVIDESYLKSSDLDLVSNLKAKAPR
                     VALTFDDGPNEKTTPKALEILKRYNAKATFFIMGQSAVGHTDILQRMHAEGHEIGNHT
                     WDHPNLTKLPAEKIKEEIHKTNDLIMKATGQKPVYLRPPYGATNATVKTVTGLKEMLW
                     SVDTEDWKNHNTQAMMTNIKKQLRPGGVILMHDIHQTSIDALPTIMDYLTTQGYYFVT
                     VGELYSTP"
BASE COUNT          766 a          421 c          365 g          801 t
ORIGIN      
        1 caatgacaac atataaaata acaagaggat tcttatgata acaaaatttc cagaccaatg
       61 gcaggataaa ttaacccagc gtcaatttga tgatctaaca gatattcaaa ataaactttt
      121 tcaacctatt actgatggtg ataatatcct tggaattagc cctacaggta ctggtaaaac
      181 tctagcatac cttttcccaa cgcttcttaa gttgcaacct aaaaagagcc aacaactctt
      241 aattttagct ccaaattcag aattagctgg acaaatcttc gacgttacaa aagaatgggc
      301 tgaaccatta ggactgacag cacaactatt cctttcaggg tctagtcaaa aacgccaaat
      361 tgagcgacta aaaaaaggac cagaaatcct tataggaact gctggacgcg tatttgaact
      421 tgtgaaacta aaaaaaataa aaatgatgaa cattaatact atcgtccttg atgagttcga
      481 tgaattactc ggcgactcac aatatcattt tgtggacaat attattaatc gcgttcctcg
      541 tgatcagcaa atgatttaca ttagtgccac caacaaactg gacaatagta aacttgccga
      601 taacactata acaattgacc tatctgatca aaaacttgat actataaaac attattatat
      661 tacagttgat aaaagagagc gtactgactt acttcgtaaa ttttcaaata ttcctgattt
      721 tagaggtctt gtctttttta acagcttatc agacttaggt gcatgtgaag aacgtttaca
      781 atttaatagg gcctcggcag tttctttagc aagcgatatt aatattaaat tccgtaaagt
      841 tatcttagag aaatttaaaa accatgatat ttcattactc ctaggaactg atttggtagc
      901 tcgtggcatt gatatcgaca acctagaata tgtgattaat tttgatatcg cacgcgacaa
      961 agaaacatat acacatcgtt ctggacgaac tggtcgtatg gggaaagagg gatgtgttat
     1021 tacatttgta acacataaag aagagctcaa acaacttaaa aaatacgcca ccgttgcaga
     1081 acttgtactt cacaatcaaa aattacactt aaaataaaaa agaaacaagt aacttgtttc
     1141 tttttttatt aaggcgttga atataattca cctacagtaa caaaataata accctgggtt
     1201 gtcagataat ccataattgt aggcaaagca tctatggatg tctggtgaat atcatgcatc
     1261 aaaatcactc ccccaggacg taattgtttt ttgatattgg tcatcattgc ttgtgtatta
     1321 tgatttttcc agtcttctgt atcaactgac cacaacattt ctttcaatcc tgtcactgtt
     1381 tttacagtag catttgttgc tccgtaaggg ggacgtaagt acactggttt ttgtcctgtt
     1441 gctttcatga ttagatcatt agtcttatga atctcttcct tgattttctc cgctggtagt
     1501 ttggttaaat taggatgatc ccatgtatga ttaccaatct catgaccttc agcatgcatc
     1561 ctttgtaaaa tatctgtgtg ccctacagca ctttgtccca ttataaagaa agtcgcttta
     1621 gcattatatc ttttaaggat ttctaaggct ttaggagtcg ttttttcatt tggtccatca
     1681 tcaaaggtaa gtgcaacacg tggtgcttta gcttttaagt tagaaactaa atctagatca
     1741 cttgatttta aatatgactc atcgatcaca ttatataatt tatcaagtgg taattttaaa
     1801 tcattaataa ttaacttacc atctttaaca tctagcccat ctgttactct tccagttttt
     1861 aaaaattctt tattgctatc atgttccttt agttgagttc ctggagctat attttctaaa
     1921 atagccttaa atccagatat attatgagct aaaagctcat ctaattcaaa aggtttctta
     1981 ttttgatcaa gtaagtaggt tctcaaaagg cgacttgatt tttgaaacat acttcctttt
     2041 tctttttgat ataagatttg ataaatggat aattttttaa catttgcaaa gttagaatct
     2101 gctaattgag gacgtagaat aatgtattga ttgtcataaa attttttatg ctcaaaactt
     2161 ttttgattcc actttaaaat atcagcgctt agacttttgt catatccttt aggttcaaga
     2221 aaaagaacac gttcagtttt ctgtcttttc accacttcta ccgttttctc ggctatgcga
     2281 atttcttttt caacaacagc ttgttgaagt tttatttcct tatgtttcaa aaacgaaaaa
     2341 ccttgccaag caa
//
LOCUS       DBJYSH010000165         2341 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.165, whole genome shotgun sequence.
ACCESSION   DBJYSH010000165 DBJYSH010000000
VERSION     DBJYSH010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2341
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.165"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            64..537
                     /locus_tag="ACUBV3_001929"
     CDS             64..537
                     /locus_tag="ACUBV3_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HHU3848398.1"
                     /translation="MFAVTQDILFIDAHSQEELFDLVSKALIKQHYVSPNYRQAVKER
                     EREFPTGLKIDLKDGTPIQYVAIPHTETQYCLVDRIFYVKNSQPITFKHMINPEEECR
                     VQDFFFIINSRNSNQSDILSNLITFFITKGNLDRLHELGDNKEKINHYLIEKGVF"
     gene            539..844
                     /locus_tag="ACUBV3_001930"
     CDS             539..844
                     /locus_tag="ACUBV3_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HHU3848399.1"
                     /translation="MIKILAACGAGVNSSHQIKDAIETQLGDRGYNVHCDAVMVKDIT
                     EEMVNKYDIFTPIAKTDLGFNVPIPVVEAGPILYRIPVMSEPVFTALEQVIKEHNLS"
     gene            884..2332
                     /locus_tag="ACUBV3_001931"
     CDS             884..2332
                     /locus_tag="ACUBV3_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001195826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HHU3848400.1"
                     /translation="MQVFLNIVNKFFDPIIHMGSGVVMLIVMTGLAMIFGVKFSKALE
                     GGIKLAIALTGIGAIIGILTGAFSESLQAFVKNTGINLSIIDVGWAPLATITWGSPYT
                     LYFLLIMLIVNIVMIVMKKTDTLDVDIFDIWHLSITGLLIMWYAKKNNLPTLLSVIIA
                     TVAIIFVGVLKIINSDLMKPTFDDLLGTGPTSPMTSTHMNYMMNPIIMVLDKLFDKVF
                     PGLDKYDFDAAKLNKAIGFWGSKFFIGMILGLVIGIMGNPVFSFAALGGWFSLGFTAG
                     ACLELFSLIGSWFIAAVEPLSQGITNFANGKMHGRRFNIGLDWPFIAGRAEIWACANI
                     LAPIMLVEAILLSKVGNGILPLAGIIAMGVTPALLVVTRGRLIRMITFGTLLLPLFLL
                     SGTMIAPFATELAKKVGAFPAGARAGSLITHSTLEGPMEKIFGYVIGKATTGQLSAII
                     TLIIFATAYLGLFMWYAKQMKRRNAEYAANQK"
BASE COUNT          713 a          370 c          456 g          802 t
ORIGIN      
        1 agataagttg ataaggcttg tttgccttac aacaaaaatg taaccgctta actaagaaga
       61 aaggtgtttg ctgtgactca agatatttta tttattgatg cccattctca ggaagaatta
      121 tttgacttag tatcaaaagc attgataaaa caacactatg ttagtcctaa ctaccgacag
      181 gcagtaaaag aaagggagag agagtttcca acggggttaa aaattgattt aaaagatggg
      241 actcctattc aatatgttgc tattccacac actgaaacgc agtattgtct agtagataga
      301 atcttttatg tcaaaaatag tcagccaatt actttcaaac atatgattaa tccagaagag
      361 gagtgtaggg ttcaggattt tttcttcatc attaatagta ggaatagtaa ccaaagtgat
      421 attttatcta atttgattac cttctttatt acaaaaggaa acctagatcg gctacatgag
      481 cttggcgaca ataaagaaaa aattaatcat tatttaatag aaaaaggagt gttttaacat
      541 gatcaaaata ttagcagctt gtggtgcagg cgtaaactca agtcatcaaa ttaaagatgc
      601 tatcgagaca caattaggag atagaggtta caatgtacat tgtgatgctg ttatggttaa
      661 ggatatcact gaggaaatgg tcaacaaata tgatatcttt actcctattg ctaaaactga
      721 tttaggtttt aatgtaccaa ttccagttgt agaagcagga cctattcttt atcgcatacc
      781 agttatgagc gaaccggtct ttactgcttt agaacaagtt atcaaagaac acaatctatc
      841 ataacatatt taaaattgga attagtctta aaggagaagg aacatgcagg tatttttaaa
      901 tattgtcaat aaattctttg atccaattat ccatatgggt tcgggagttg tgatgctaat
      961 tgtcatgaca ggtttagcca tgatatttgg agtgaagttt tctaaagcac ttgaaggtgg
     1021 tattaagtta gctattgctc ttacgggtat tggtgctatt attggtattt taactggtgc
     1081 tttttctgaa tcacttcaag cttttgttaa aaatacagga atcaatctaa gcattattga
     1141 cgttggttgg gctccattag caactattac atggggatca ccatatacgc tttacttctt
     1201 attaatcatg cttattgtca atattgttat gattgttatg aaaaaaacgg ataccttaga
     1261 tgttgatatt tttgatattt ggcatttatc tatcacaggt cttttaatta tgtggtatgc
     1321 taaaaaaaat aatctaccaa cacttttatc tgtcattatt gcaactgtag ctattatctt
     1381 cgttggtgtt ctaaaaatta tcaattctga tttgatgaaa cctacctttg atgatttatt
     1441 ggggacaggt ccaacttctc ccatgacttc aacgcatatg aattatatga tgaaccccat
     1501 tattatggtt ttggataagc tttttgataa agtttttcca gggctagata aatatgactt
     1561 tgacgcggct aaattaaata aagccattgg attctggggc tctaagttct ttattggtat
     1621 gattttagga cttgtcatag ggattatggg caaccccgtg ttctcatttg cagcattagg
     1681 tggctggttt tctctcggtt ttacagcagg tgcttgttta gagttgttct ctttgattgg
     1741 ttcttggttc attgctgcag tagaaccact ttctcaagga attactaatt ttgctaatgg
     1801 taaaatgcat ggtcgtcgtt tcaatattgg tttggactgg ccatttattg ccggacgtgc
     1861 agagatttgg gcatgtgcta acattcttgc accgattatg ctagtagaag ctattttact
     1921 ctctaaagtt ggtaatggta ttctaccgct tgctggtatt atcgcaatgg gcgtaacccc
     1981 tgctttactg gttgttacac gtggccgttt aattcgtatg attacctttg gaacattgct
     2041 attaccgcta tttcttttat caggaacaat gattgcccca tttgcaactg aattagctaa
     2101 aaaggtaggc gcattcccag ctggggcgcg agcaggttca cttatcactc actctacact
     2161 agaaggccct atggagaaaa tctttggtta cgttattggt aaagcgacaa caggtcaatt
     2221 atcagcgatt ataacattaa ttatttttgc gacagcttat cttggtttgt ttatgtggta
     2281 tgctaaacaa atgaaacgcc gtaatgcaga atatgcagct aatcaaaaat aaatagaata
     2341 a
//
LOCUS       DBJYSH010000166         2312 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.166, whole genome shotgun sequence.
ACCESSION   DBJYSH010000166 DBJYSH010000000
VERSION     DBJYSH010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2312
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.166"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            <1..819
                     /locus_tag="ACUBV3_001932"
     CDS             <1..819
                     /locus_tag="ACUBV3_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HHU3848401.1"
                     /translation="NMIYGFVGPNGAGKTTTIKMILGLLKFDTGFITIFGKKVNFGRT
                     DTNQLIGYLPDVPEYYDYMTALEYLDLCSGLARSKHKLSNKELLRSVGLDDNHQKIAT
                     YSRGMKQRLGLAQALVHDPKIIICDEPTSALDPKGRQDILDIISNLRGEKTVIFSTHI
                     LSDVEKICDHVLVLTKCGIYSLEELKGKKSEENYSVRILIKVTKSEAKVLSHNYQIEK
                     KDNEYALTLKGSKMDNKADLLAGFYQDLVSLKISPSAIEVIDNSLEELYLEVTK"
     gene            816..1577
                     /locus_tag="ACUBV3_001933"
     CDS             816..1577
                     /locus_tag="ACUBV3_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HHU3848402.1"
                     /translation="MKSLAVMLKKEWMENVRTYKVISILITCSIFGILGPLTALMMPD
                     IMAGILPKKLQGAIPEPTYIDSYIQYFKNMNQLGLVILVFLFSSTLTQEFSKGTLINL
                     VTKGLAKKVIILAKFIVITLLWTVSYLLSVVIHFSYTLYYFSNEGSHKLMVYGATWFI
                     GILFISLILFFSVLFRKTLGGLLGCLFMVVLCVVSGFFKISKSYNPMLLIQKQGEILS
                     GKFKVQDLMEPSILVFLLIIMTLLLSMYIFEKSEV"
     gene            1746..2234
                     /locus_tag="ACUBV3_001934"
     CDS             1746..2234
                     /locus_tag="ACUBV3_001934"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HHU3848403.1"
                     /translation="MEIKEYENNPYHLAQLVDLINYCQNIEAKLDIKMAEQDDIFQIE
                     NYYQNRKGQFWIALENEKVVGSIALLRIDDKTAVLKKFFTYPKYRGNPVRLGRKLFER
                     FMLFARASKFTRIVLDTPEKEKRSHFFYENQGFKQITRDELDVDYIFPDRDSRIYVKL
                     LD"
BASE COUNT          827 a          308 c          401 g          776 t
ORIGIN      
        1 aatatgattt atggattcgt aggaccaaat ggagcaggta aaacaacgac aataaaaatg
       61 attttaggat tgttaaaatt tgatacgggt ttcataacaa tttttggtaa aaaagttaat
      121 tttggaagaa cagataccaa tcaactgatt ggctatctcc cagatgttcc cgagtactac
      181 gattatatga cagctctaga atatcttgat ttatgttctg gtttagcgag atcaaagcat
      241 aagctttcta acaaagaact acttaggtca gttggacttg acgataatca ccaaaagatt
      301 gctacttatt ctcgaggaat gaaacagcgt cttggattag cccaagcact ggttcatgat
      361 cctaaaatta ttatttgcga tgaaccaacc tctgcattag acccgaaagg tcgccaagat
      421 attttagata ttatttcaaa tcttagagga gaaaaaactg tgattttttc tacccatatt
      481 ctctctgatg tcgaaaaaat atgtgatcat gttctggtgt taactaagtg tgggatttat
      541 tctcttgaag agttaaaagg aaaaaaatca gaggaaaatt attcggttcg tattttaata
      601 aaagtgacaa agtctgaagc taaggtacta tctcataact atcagataga aaagaaagat
      661 aatgagtatg cactgactct caaaggctct aaaatggata acaaagcaga tttactagct
      721 ggtttttacc aagatttagt ttctttaaag attagcccat cagctattga agtcattgac
      781 aatagtttag aggagcttta tttggaggtt accaaatgaa atctttagca gtaatgttaa
      841 aaaaagaatg gatggagaat gttaggacat acaaagttat atctattctg attacttgtt
      901 caatatttgg tatattaggt cccttaactg cacttatgat gcctgatatt atggcaggaa
      961 ttttacctaa aaaactacag ggagcgatac cagagcctac ctatattgat tcttatattc
     1021 agtattttaa aaatatgaat cagcttggtt tagttatttt agtattttta tttagtagta
     1081 cattgacgca agaattttca aaaggaacct taattaatct agttacgaag ggattagcca
     1141 aaaaagttat aatccttgct aaatttatag taattaccct tctatggaca gtgtcatact
     1201 tattaagtgt tgtcatccat tttagttata cgttatatta ctttagtaac gaaggaagtc
     1261 ataaattaat ggtttacggt gcaacatggt ttatcggtat tcttttcata agcttaatac
     1321 tctttttttc agttcttttt cgtaaaactt tagggggttt gttaggttgt ctttttatgg
     1381 tggtgttatg tgtagtatct ggatttttta aaatcagtaa aagctataat cctatgcttc
     1441 ttatccaaaa acaaggagaa atattaagtg gaaagtttaa agttcaagac ttaatggaac
     1501 cgagcatatt agtgtttttg cttattataa tgacgttatt acttagcatg tacatttttg
     1561 aaaaaagtga agtttaattt tgataagata tcaataaggc gatagctact ttttgaaaaa
     1621 ataaaaccga attaagagct ttgctgctat ttgacttatc tagatcaata tttccaacta
     1681 atatgatatc ctatgcatac ctcattgcag aaatatactt taaagtcaaa ataaggaaat
     1741 atattatgga aattaaagaa tacgagaata atccgtacca tttagcacag ctagtagatt
     1801 taattaatta ttgtcagaat atagaagcta aacttgatat aaagatggct gaacaagacg
     1861 atatttttca aatagaaaat tattatcaaa acagaaaagg tcaattttgg attgccttag
     1921 aaaatgaaaa agtagttggt tcgattgcgt tactgcgtat tgatgataaa acagctgttc
     1981 taaaaaaatt ttttacatat ccaaaatata gaggaaatcc tgttcgactt ggtcgaaaat
     2041 tatttgaaag atttatgctt tttgctcgag ctagtaaatt cacaagaatt gtcttggata
     2101 ctccagaaaa agagaaaaga tctcacttct tttatgaaaa tcaaggattt aagcaaataa
     2161 caagagatga actagatgta gactacattt ttccagatag agattcaagg atatatgtaa
     2221 aacttcttga ttaattgtga aaaaaactag tcaaacagat gaataaaccc aaataacgat
     2281 tattttagat aatacattgg aaaagtggtt gt
//
LOCUS       DBJYSH010000167         2185 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.167, whole genome shotgun sequence.
ACCESSION   DBJYSH010000167 DBJYSH010000000
VERSION     DBJYSH010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.167"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            <1..>2185
                     /locus_tag="ACUBV3_001935"
     CDS             <1..>2185
                     /locus_tag="ACUBV3_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063250.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HHU3848404.1"
                     /translation="NEKKVKYFLRKTAYGLASMSAAFAVCSGIVHADTSSGISASIPH
                     KKQVNLGAVTLKNLISKYRGNDKAIAILLSRVNDFNRASQDTLPQLINSTEAEIRNIL
                     YQGQIGKQNKPSVTTHAKVSDQELGKQSRRSQDIIKSLGFLSSDQKDILVKSISSSKD
                     SQLILKFVTQATQLNNAESTKAKQMAQNDVALIKNISPEVLEEYKEKIQRASTKSQVD
                     EFVAEAKKVVNSNKETLVNQANGKKQEIAKLENLSNDEMLRYNTAIDNVVKQYNEGKL
                     NITAAMNALNSIKQAAQEVAQKNLQKQYAKKIERISSKGLALSKKAKEIYEKHKSILP
                     TPGYYADSVGTYLNRFRDKQTFGNRSVWTGQSGLDEAKKMLDEVKKLLKELQDLTRGT
                     KEDKKPDVKPEAKPEAKPNIQVPKQAPTEAAKPALSPEALTRLTTWYNQAKDLLKDDQ
                     VKDKYVDILAVQKAVDQAYDHVEEGKFITTDQANQLANKLRDALQSLELKDKKVAKPE
                     AKPEAKPDVKPEAKPDVKPEAKPDVKPEAKPDVKPEAKPDVKPEAKPDVKPKAKPDVK
                     PEAKPDVKPDVKPDVKPEAKPEAKPDVKPDVKPEAKPDVKPEAKPEAKPEAKPEAKPE
                     AKPEAKPEAKPDVKPEAKPDVKPEAKPEAKPEAKSEAKPEAKLEAKPEAKPATKKSVN
                     TSGNLAAKKAIENKKYSKKLPSTGEAASPLLAIVSLIVMLSAGLIT"
BASE COUNT          886 a          389 c          422 g          488 t
ORIGIN      
        1 taacgaaaaa aaagtaaaat actttttaag aaaaacagct tatggtttgg cctcaatgtc
       61 agcagcgttt gctgtatgta gtggtattgt acacgcggat actagttcag gaatatcggc
      121 ttcaattcct cataagaaac aagttaattt aggggcggtt actctgaaga atttgatttc
      181 taaatatcgt ggtaatgaca aagctattgc tatactttta agtagagtaa atgattttaa
      241 tagagcatca caggatacac ttccacaatt aattaatagt actgaagcag aaattagaaa
      301 tattttatat caaggacaaa ttggtaagca aaataaacca agtgtaacta cacatgctaa
      361 agttagtgat caagaactag gtaagcagtc aagacgttct caagatatca ttaagtcatt
      421 aggtttcctt tcatcagacc aaaaagatat tttagttaaa tctattagct cttcaaaaga
      481 ttcgcaactt attcttaaat ttgtaactca agccacgcaa ctgaataatg ctgaatcaac
      541 aaaagctaag caaatggctc aaaatgacgt ggccttaata aaaaatataa gccccgaagt
      601 cttagaagaa tataaagaaa aaattcaaag agctagcact aagagtcaag ttgatgagtt
      661 tgtagcagaa gctaaaaaag ttgttaattc caataaagaa acgttggtaa atcaggccaa
      721 tggtaaaaag caagaaattg ctaagttaga aaatttatct aacgatgaaa tgttgagata
      781 taatactgca attgataatg tagtgaaaca gtataatgaa ggtaagctca atattactgc
      841 tgcaatgaat gctttaaata gtattaagca agcagcacag gaagttgccc agaaaaactt
      901 acaaaagcag tatgctaaaa aaattgaaag aataagttca aaaggattag cgttatctaa
      961 aaaggctaaa gaaatttatg aaaagcataa aagtattttg cctacacctg gatattatgc
     1021 agactctgtg ggaacttatt tgaataggtt tagagataaa caaactttcg gaaataggag
     1081 tgtttggact ggtcaaagtg gacttgatga agcaaaaaaa atgcttgatg aagtcaaaaa
     1141 gcttttaaaa gaacttcaag accttaccag aggtactaaa gaagataaaa aaccagacgt
     1201 taagccagaa gccaaaccag aggccaaacc aaatattcaa gtacctaaac aagcacctac
     1261 agaagctgca aaaccagctt tgtcaccaga agccttgaca agattgacta catggtataa
     1321 tcaagctaaa gatctgctta aagatgatca agtaaaggac aaatacgtag atatacttgc
     1381 agttcaaaaa gctgttgacc aagcttatga tcatgtggaa gagggaaaat ttattaccac
     1441 tgatcaagca aatcaattag ctaacaagct acgtgatgct ttacaaagtt tagaattaaa
     1501 agataaaaaa gtagccaaac cagaagccaa accagaggcc aaaccagacg ttaagccaga
     1561 ggccaaacca gacgttaagc cagaagctaa gccagacgtt aaaccagaag ctaagccaga
     1621 cgttaaacca gaagctaagc cagacgttaa accagaagct aagccagacg ttaaaccaaa
     1681 ggccaaacca gacgttaagc cagaagctaa gccagacgtt aaaccagacg ttaaaccaga
     1741 cgttaagcca gaggccaaac cagaggctaa gccagacgtt aaaccagacg ttaagccaga
     1801 agctaaacca gacgttaagc cagaggccaa accagaagct aagccagaag ctaagccaga
     1861 agctaagcca gaagctaagc cagaggccaa accagaagct aagccagacg ttaagccaga
     1921 agctaaacca gacgttaaac cagaggctaa gccagaagct aaaccagagg ctaagtcaga
     1981 agctaaacca gaggctaagc tagaagctaa accagaggcc aaaccagcaa ccaaaaaatc
     2041 ggttaatact agcggaaact tggcggctaa aaaagctatt gaaaacaaaa agtatagtaa
     2101 aaaattacca tcaacgggtg aagccgcaag tccactctta gcaattgtat cactaattgt
     2161 tatgttaagt gcaggtctta ttacg
//
LOCUS       DBJYSH010000168         2166 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.168, whole genome shotgun sequence.
ACCESSION   DBJYSH010000168 DBJYSH010000000
VERSION     DBJYSH010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2166
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.168"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            complement(109..1032)
                     /locus_tag="ACUBV3_001936"
     CDS             complement(109..1032)
                     /locus_tag="ACUBV3_001936"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000964166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HHU3848405.1"
                     /translation="MLVYIAGSGAMGCRFGYQISKTNHDVILLDNWADHIMAIKENGL
                     KVTGDTEDLVKLPIMKPTDATEEADLIILFTKAMQLPNMLQDIKKIIGKKTKVLCLLN
                     GLGHEDVIRQYIPEHNILMGVTVWTAGLKGPGHAHLEGVGSVNLQSIDPNNQEAGYRV
                     TELLNEAKLQATYDENVLPNIWRKACVNGTMNSTCALLDCTIGQLFASEDGVNMVHEI
                     IHEFVTVGKAEGVELDEEEITKYVMDTSVKAAHHYPSMHQDLVQNQRLTEIDFLNGAV
                     NKKGENLGIDTPYCRLITQLIHTKENVLSIK"
     gene            complement(1045..2115)
                     /locus_tag="ACUBV3_001937"
     CDS             complement(1045..2115)
                     /locus_tag="ACUBV3_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HHU3848406.1"
                     /translation="MTNTVTPKETAGSFINKVLGGTATAIVVALIPNAILATFLKPFL
                     SYGLAAEFLHIVQVFQFFTPIMAGFLIGQQFKFTPMQQLAVGGAAYIGSGAWAYTEVI
                     QKGVATGSFQLRGIGDLINMMLTAALAVLAVKWFGNKFGSLTIILLPIIIGTGVGYLG
                     WKLLPYVSYVTTLIGQGINSFTTLQPIAMSILIAMAFSMLIVSPISTVAIGLAIGLNG
                     MSASAASMGVASTTAVLVWATMKANKSGVPIAIALGAMKMMMPNFLKHPVMAIPMLMT
                     ATVSSLTVPLFKLVGTPASSGFGLVGAVGPIASFEAGASMLIVILSWLVIPFAVGFVS
                     HKICKDILKLYKDDIFVFEGQN"
BASE COUNT          706 a          451 c          344 g          665 t
ORIGIN      
        1 ctgtaaatac ttctcgaaaa aaatacccaa ctcttattat tcttagtata acaaaatcat
       61 tatcattaag catatcaaaa aaccgggcac tattctcccg gttgattatt attttatgct
      121 aagtacattt tctttagtgt gaattaattg tgtgattaat cgacagtaag gtgtatcaat
      181 gcctaggttt tcaccttttt tattcactgc gccatttaag aaatcaattt cagttaagcg
      241 ctgattttga actaagtctt gatgcattga agggtaatga tgggctgctt taacagatgt
      301 atccataacg tactttgtta tttcttcttc atcaagctct acaccttctg cttttccaac
      361 agtaacaaat tcatggataa tttcatgtac catatttacg ccgtcttcac tagcaaagag
      421 ttgaccaatt gtacaatcta gcaaggcgca agttgaattc atcgttccgt taacacaagc
      481 ttttctccaa atatttggca aaacattttc gtcataagta gcctgtaatt ttgcttcatt
      541 aagcaattca gtaacgcgat aacctgcttc ttgattgtta ggatcgatac tttgaagatt
      601 tacagaacca actccttcca gatgagcatg ccctggtcct ttcaaccctg cagtccaaac
      661 tgttactccc atcaaaatat tgtgttcagg gatatattga cgaatgacat cttcatgtcc
      721 taatccattt aaaagacata agacctttgt tttcttacca ataatttttt taatatcttg
      781 aagcatattt ggcaactgca ttgctttagt aaacaaaata attaaatctg cttcttctgt
      841 cgcatcagtt ggcttcatga taggaagttt aacaaggtcc tctgtatcac ctgttacctt
      901 caagccattt tctttaatag ccataatatg gtctgcccaa ttatcaagta aaataacatc
      961 atgatttgtt ttagaaattt gatatccaaa acgacatccc atagcgcctg aaccagcaat
     1021 ataaactaac atacaacctc caaattaatt ttgaccttca aaaacgaaaa tatcgtcttt
     1081 ataaagtttc agaatgtctt tacaaatttt gtgtgataca aaacctactg caaaaggaat
     1141 aacgagccaa gataagataa caatcaacat acttgctcct gcttcaaatg aagcaatcgg
     1201 acctactgca ccaacaagcc caaaaccaga cgaagctggt gtccccacca atttaaacaa
     1261 tgggactgtt agagaactta ctgtagcagt cataagcatt ggaatcgcca ttactggatg
     1321 ctttaggaaa tttggcatca tcatcttcat tgcacctaaa gcaatagcaa ttggaactcc
     1381 agacttatta gccttcatcg ttgcccaaac tagcactgct gttgttgaag caactcccat
     1441 tgaagctgca cttgctgaca taccattaag accaatcgct agaccaatcg ctactgttga
     1501 aataggactt acaataagca ttgaaaaagc catcgcaatt agaattgaca tcgcaattgg
     1561 ttgtaaagta gtaaaagaat taattccttg accaataagt gttgtcacat atgaaacgta
     1621 agggagtaat ttccatccta aatagcctac gcctgtacct atgataatag gtaacaagat
     1681 aatggttagg gaaccaaatt tattaccaaa ccacttaact gctaaaactg ctaaagctgc
     1741 tgttagcatc atattgatga gatcaccaat cccacgcaat tggaaactac cagtagcaac
     1801 tcctttttga ataacttctg tgtaagccca cgcaccagaa ccaatatatg cagcacctcc
     1861 aacagcaagc tgttgcattg gggtgaattt aaactgttga ccaatcaaaa atcctgccat
     1921 aattggtgtg aaaaattgga atacctgcac gatgtgtaag aactctgcag caagtccgta
     1981 agataaaaat ggtttcaaaa aagtggctag gatagcattt gggatgagtg ctacaacgat
     2041 tgcagttgct gtcccaccta acactttatt gataaaagag ccagccgtct cttttggtgt
     2101 taccgtattt gtcataaaat acctcgcttc atttatttaa tatcattcat aacgacaaag
     2161 ttaatt
//
LOCUS       DBJYSH010000169         2161 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.169, whole genome shotgun sequence.
ACCESSION   DBJYSH010000169 DBJYSH010000000
VERSION     DBJYSH010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.169"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            87..2126
                     /locus_tag="ACUBV3_001938"
     CDS             87..2126
                     /locus_tag="ACUBV3_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HHU3848407.1"
                     /translation="MKRKYFVLNTVTVLTLATAMNTSSIYANSTETSASVAPTTNTIV
                     QTNDSNPTAKFASESGQSVIGQVKPANSAALTTVDTPHISAPDALKTTQSSPVVESPS
                     TKLTEETYKQKDGQDLANMVRSGQVTSEELVNMAYDIIAKENPSLNAVITTRRQEAIE
                     EARKLKDTNQPFLGVPLLVKGLGHSIKGGETNNGLIYADGKISTFDSSYVKKYKDLGF
                     IILGQTNFPEYGWRNITDSKLYGPTHNPWNLAHNAGGSSGGSAAAIASGMTPIASGSD
                     AGGSIRIPSSWTGLVGLKPTRGLVSNEKPDSYSTAVHFPLTKSSRDAETLLTYLKKSD
                     QTLVSVNDLKSLPIAYTLKSPMGTEVSQDAKNAIMDNVTFLRKQGFKVTEIDLPIDGR
                     ALMRDYSTLAIGMGGAFSTIEKDLKKHGFTKEDVDPITWAVHVIYQNSDKAELKKSIV
                     EAQKHMDDYRKAMEKLHKQFPIFLSPTTASLAPLNTDPYVTEKDKRAIYNMENLSQEE
                     RIALFNRQWEPMLRRTPFTPIANMTGLPAISIPTYLSESGLPIGTMLMAGANYDMVLI
                     KFATFFEKHHGFNVKWQRIIDKEVKPSADLIQPTNSLFKAHSSLVNLEENSQVTQVSI
                     SKKWMKSSVKNKPSVMAYQKALPKTGDTESSLSPVLVVTLLLACFSFVTKKNQKS"
BASE COUNT          759 a          374 c          396 g          632 t
ORIGIN      
        1 aatataaaat aacagttaat attttagcaa tttgataaca cttagattat aatgtaaacg
       61 tatacttttt tagattggaa gatgaaatga aacgtaaata ctttgttctt aatacggtga
      121 cggttttaac gttagctact gcaatgaata ctagcagtat ctatgctaat agtactgaga
      181 caagtgcttc agtagctcct actacaaata ctatcgttca aactaatgac agtaatccta
      241 ccgcaaaatt tgcatcagaa tcaggacaat ctgtaatagg tcaagtaaaa ccagctaatt
      301 ctgcggcgct tacaacagtt gacacgcctc atatttcagc tccagatgct ttaaaaacaa
      361 ctcaatcaag tcctgtcgtt gagagtcctt ctactaagtt aactgaagag acatacaaac
      421 aaaaagatgg tcaagattta gccaacatgg tgagaagtgg tcaagttact agtgaggaac
      481 tcgtcaatat ggcatacgat attatcgcta aagaaaaccc atctttaaat gcagtcatta
      541 ctactagacg ccaagaagcc attgaagagg ctagaaaact taaagatact aatcagccgt
      601 ttttaggtgt tcccttgtta gtcaaggggt tagggcacag tattaaaggt ggtgaaacca
      661 ataatggctt gatctatgca gatggaaaaa ttagcacatt tgacagtagc tatgtcaaaa
      721 aatataaaga tttaggattt attattttag gacaaacgaa ttttccagag tatgggtggc
      781 gtaatataac agactctaaa ttatacggtc caacgcataa tccttggaat cttgctcata
      841 acgctggtgg ctcttctggt ggaagtgcag cagctattgc tagcggaatg acgccaattg
      901 ctagcggcag tgatgctggt ggttctatcc gtattccatc ttcttggacg ggcttagtag
      961 gtttaaaacc aacaagagga ttggtgagta atgaaaagcc agattcgtat agtacagcag
     1021 ttcattttcc attaactaag tcatctagag acgcagaaac attgttaact tacctaaaga
     1081 aaagcgatca aacgctagta tcagttaatg atttaaaatc tttaccaatt gcttatactt
     1141 tgaaatcacc aatgggaaca gaagttagtc aagatgctaa aaatgctatt atggacaacg
     1201 tcacattctt aagaaaacaa ggattcaaag tgacagagat agatttacca attgatggta
     1261 gagcattaat gcgtgattat tcaaccttgg ctattggcat gggaggagct ttttcaacaa
     1321 ttgaaaaaga cttaaaaaaa catggtttta ctaaagaaga cgttgatccc attacttggg
     1381 cagttcatgt tatttatcaa aattcagata aggctgaact taagaaatct attgtggaag
     1441 cccaaaaaca tatggatgat tatcgtaagg caatggagaa gcttcacaag caatttccta
     1501 ttttcttatc gccaacgacc gcaagtttag cccctctaaa tacagatcca tatgtaacag
     1561 agaaagataa aagagcgatt tataatatgg aaaacttgag ccaagaagaa agaattgctc
     1621 tctttaatcg ccagtgggag cctatgttgc gtagaacacc ttttacacca attgctaata
     1681 tgacaggact cccagctatc agtatcccga cttacttatc tgagtctggt ttacccatag
     1741 ggacgatgtt aatggcaggt gcaaactatg atatggtatt aattaaattt gcaactttct
     1801 ttgaaaaaca tcatggtttt aatgttaaat ggcaaagaat aatagataaa gaagtgaaac
     1861 catctgctga cctaatacag cctactaact ccctctttaa agctcattca tcattagtaa
     1921 atttagaaga aaattcacaa gttactcaag tatctatctc taaaaaatgg atgaaatcgt
     1981 ctgttaaaaa taaaccatcc gtaatggcat atcaaaaagc acttcctaaa acaggtgata
     2041 cagaatcaag tctatctcca gttttagtag taaccctttt attagcttgt tttagctttg
     2101 taacaaaaaa gaatcagaaa agttgattgt aatgtaaaat aattcctatt tttcactttt
     2161 t
//
LOCUS       DBJYSH010000170         2154 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.170, whole genome shotgun sequence.
ACCESSION   DBJYSH010000170 DBJYSH010000000
VERSION     DBJYSH010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.170"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            <1..2140
                     /locus_tag="ACUBV3_001939"
     CDS             <1..2140
                     /locus_tag="ACUBV3_001939"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HHU3848408.1"
                     /translation="KIKDFASRAINTRLGFILLLVVIYWLKTIWAYHTDFNLGLENSY
                     QLFLTIINPIPLGLLIIGLALYVKRTKAFYITAFITYAIVNILLIANAIYYREFSDFI
                     TVSAVLASSKTSAGLGDSALNLLRIWDLVYVFDFIILIFLFATKKIHLDNRPFNKRAS
                     FSITALSGLLFSINLFLAEIDRPELLSRGFSNTYIVKALGLPSFSIYSGNQTYQAQKE
                     RNGATAQELATAKKYVAEHYAKPNPEYYGIGKGRNVIMIHLESFQQFLIDYKLNVDGK
                     EHVVTPFINSLYHSKETISFSNFFHQVKAGKTSDAETLMENSLFGLSSGSFMVNYGGE
                     NTQFAAPHILAQNGGYSSAVFHGNVGTFWNRNNAYKQWGYDYFFDSSYFSKQTKDNSF
                     QYGLNDKYMFADSIKYLEHMQQPFYTKFITVSNHYPYTSLKGESDEEGFPLAKTNDET
                     INGYFATANYLDTALKSFFEYLKAAGVYDNSIIVMYGDHYGISNTRNPSLAELLGKDP
                     ETWSEYDNAMLQRVPYMIHIPGYSKGFISNTYGGEVDNLPTLLHILGIDTSKYTQLGQ
                     DLLSKDNKQMVAMRTTGQYITPKYTNYSGHLYYTDSGQEITNPDETTKAEIKTIRDAT
                     NKQLSTSDSIQTGDLLRFDENNGLKTVEVEKFNYTHSLKALKAKERKLKDRSTSIYSK
                     HNNKSTVDLFHAPSYLELQDPNKTHKTSKK"
BASE COUNT          721 a          400 c          347 g          686 t
ORIGIN      
        1 aaaaattaaa gattttgcta gccgtgcgat caatacacga ctcggcttta ttttattgtt
       61 agtagttata tattggttaa agacaatttg ggcttaccat actgatttta atttaggttt
      121 agaaaattcc tatcagttgt ttttgaccat tataaacccc atccccttag gactcttaat
      181 cataggtttg gccttatatg ttaagcgaac caaagctttt tatattaccg cttttatcac
      241 ctatgctatc gtaaatatcc ttctaattgc aaacgcaatc tactatagag agttttccga
      301 tttcattaca gttagcgctg tcttggctag tagtaaaact tcggctggcc ttggagactc
      361 tgctcttaat ctattgagaa tttgggatct tgtctacgtt ttcgatttca ttatccttat
      421 tttcttattt gcaaccaaga aaatccactt agataatcgt ccatttaata aacgtgctag
      481 tttttctatc acagctttgt ctggtttact cttttcaatt aacttattct tagctgaaat
      541 tgatcgtcca gaactactaa gtcgtggttt ttcaaacacc tatattgtta aagctcttgg
      601 cttgccatca ttttcgattt atagtggtaa tcaaacttat caagcacaga aagaaagaaa
      661 tggtgccact gcacaagaat tagcgactgc caaaaaatat gtagcagagc attatgccaa
      721 acccaatcca gaatattatg gtatcggaaa agggcgaaac gttatcatga ttcatttgga
      781 gagttttcaa caattcttga ttgactataa attaaatgtt gatggtaaag aacatgtggt
      841 tactccattt attaacagcc tttatcattc gaaagagact atctccttct ctaacttctt
      901 ccatcaagtc aaagctggta agacatccga tgctgagaca ctcatggaaa actcactttt
      961 tggtctaagt agtggttcat tcatggttaa ttatggtggt gaaaatacac aatttgctgc
     1021 tccacatatc ttagctcaaa atggaggtta cagtagtgcg gtcttccacg gaaacgtcgg
     1081 aacattttgg aatagaaata atgcttacaa acagtggggt tatgactact tctttgactc
     1141 tagctacttc tcaaaacaaa ccaaagataa ttcattccaa tacggtttga atgataaata
     1201 tatgtttgct gactctatca aatatctaga gcacatgcaa caaccgtttt atactaaatt
     1261 tattacagta agtaaccatt acccttacac aagtttgaag ggagagtctg acgaagaggg
     1321 attcccactt gcaaagacta acgatgaaac gattaacggt tatttcgcca cagcaaacta
     1381 ccttgataca gctcttaaat cattttttga atatctaaaa gctgctggtg tgtatgataa
     1441 ttctattatt gtaatgtatg gggatcacta cggtatttct aatacacgca atcctagctt
     1501 agctgaatta ttaggtaaag accctgaaac atggtctgaa tacgacaacg ctatgttaca
     1561 acgtgttcca tatatgatcc acattcctgg ctattcaaaa ggttttatca gtaatacata
     1621 cggtggtgag gtagataatt taccaacctt acttcatatt ctagggatag acacgtctaa
     1681 atatactcag ttaggacaag acttgttatc taaagataat aaacaaatgg tcgctatgag
     1741 aacaactggt cagtatatta ctcctaaata cactaactat agtggacatc tctattacac
     1801 tgattcagga caagaaatca caaatcctga tgaaacaact aaagctgaaa taaaaactat
     1861 tcgagatgct actaataaac aattatctac aagtgattcg attcaaactg gagatttact
     1921 acgatttgat gaaaacaatg gtttgaaaac tgtagaagtt gaaaaattca attacacaca
     1981 ttctcttaaa gcactcaaag ctaaagaacg taaattgaaa gatcgttcaa caagcatata
     2041 cagcaaacat aacaacaaat caacagttga tctcttccac gctccaagtt acttggaatt
     2101 gcaagatcct aataagacac ataaaacatc taaaaaataa tctttaccat ttaa
//
LOCUS       DBJYSH010000171         2045 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.171, whole genome shotgun sequence.
ACCESSION   DBJYSH010000171 DBJYSH010000000
VERSION     DBJYSH010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.171"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            41..493
                     /locus_tag="ACUBV3_001940"
     CDS             41..493
                     /locus_tag="ACUBV3_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6556 family protein"
                     /protein_id="HHU3848409.1"
                     /translation="MSKGYSRQQKKSTKTNTDSTQSPAKHIKTGFSALQKTIALVGSV
                     LSIIVASITITNAINNHKDSSKTDSSTSKTVIIRESGQNSSNQVSTEKDETRASSSQT
                     LENSSASSTPSSSSNSAANNDQTSSSTNNDQTNNTTTDTSTIENTTTP"
     gene            complement(536..1885)
                     /gene="gorA"
                     /locus_tag="ACUBV3_001941"
     CDS             complement(536..1885)
                     /gene="gorA"
                     /locus_tag="ACUBV3_001941"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HHU3848410.1"
                     /translation="MSKQYDYIVIGGGSAGSGTANRAAMYGAKVLLIEGGQVGGTCVN
                     LGCVPKKIMWYGAQVSETLHKYSSGYGFEANNLSFDFTTLKANRDAYVQRSRQSYAAN
                     FERNGVEKIDGFARFIDNHTIEVNGQQYKAPHITIATGGHPLYPDIIGSELGETSDDF
                     FGWETLPNSILIVGAGYIAAELAGVVNELGVETHLAFRKDHILRGFDDMVTSEVMAEM
                     EKSGISLHANHVPKSLKRDEGGKLIFEAENGKTLVVDRVIWAIGRGPNVDMGLENTDI
                     VLNDKDYIKTDEFENTSVDGVYAIGDVNGKIALTPVAIAAGRRLSERLFNHKDNEKLD
                     YHNVPSVIFTHPVIGTVGLSEAAAIEQFGKDNIKVYTSTFTSMYTAVTSNRQAVKMKL
                     ITLGKEEKVIGLHGVGYGIDEMIQGFSVAIKMGATKADFDDTVAIHPTGSEEFVTMR"
BASE COUNT          668 a          453 c          300 g          624 t
ORIGIN      
        1 aaaatcatat aatagttgat gtacatttaa ggagggcgtc atgtcaaaag gatattcaag
       61 acaacagaaa aagtctacta agactaatac tgattcaact caatcacccg ctaagcatat
      121 aaaaacaggt ttttctgcac ttcaaaaaac aattgcatta gtcggtagtg ttttaagtat
      181 tatcgttgcc tctattacta ttactaatgc gattaataat cacaaagact ctagtaagac
      241 tgattcttct acttcaaaaa ccgtcattat aagagaaagt ggacaaaatt ctagtaacca
      301 agtttctact gaaaaagatg agacaagggc atcatccagc caaacactag aaaattcctc
      361 tgctagtagt actccgagta gcagttcaaa ttctgctgcc aataacgatc aaacatcatc
      421 tagtactaac aatgatcaaa caaataatac tactaccgat acctcaacta ttgaaaatac
      481 aacaacacct taaaaaagct aggttcccta gctttttttg tattctttga tagcgttagc
      541 gcattgtaac aaattcctca gatccagttg ggtgaatagc aacagtatca tcaaagtctg
      601 ctttagtagc ccccattttg atagcaactg aaaaaccttg aatcatttca tcaataccat
      661 aaccaacacc atgaagccca ataacttttt cctcttttcc tagggttatg agcttcatct
      721 taactgcttg gcgattactg gtaacagccg tatacataga ggtaaaagtt gatgtataga
      781 ctttgatatt atcttttcca aattgctcga tagctgctgc ttctgaaagt cctaccgtcc
      841 caattacagg gtgagtaaaa ataactgaag gtacattatg gtaatctaat ttttcgttat
      901 ctttatgatt aaaaagtctt tctgataagc gacgacctgc tgcaattgct accggtgtca
      961 aggcaatttt cccattaaca tctccaatag catacacgcc atctacagaa gtattctcaa
     1021 attcatctgt tttgatataa tctttatcat ttaaaacaat atcggtattt tcaagtccca
     1081 tgtctacatt tggtccacgg ccgatagccc atattacacg atcaacgaca agcgttttcc
     1141 cattttcagc ttcaaaaatc aacttgccac cttcatcgcg tttaagagat ttaggtacat
     1201 ggttagcatg taaagagata cctgatttct ccatttcagc cataacctca cttgttacca
     1261 tgtcatcaaa tccgcgtaga atatggtctt ttctaaatgc aagatgggtt tcaacgccta
     1321 attcattaac cactccagca agttctgccg cgatatagcc cgccccaaca atcaatatag
     1381 aatttggtaa ggtctcccat ccaaaaaaat catcagaagt ctcaccaagt tcacttccaa
     1441 taatatcagg gtaaagaggg tgtccacctg ttgcaatagt aatgtgagga gctttatatt
     1501 gctgaccatt cacttcaata gtatggttat caataaaacg agcaaatcca tcaatctttt
     1561 cgaccccatt acgctcaaaa ttagcggcat acgactgtct agaccgctgc acgtaagcat
     1621 cgcgattagc ttttagagta gtaaaatcaa aactaagatt attggcttca aaaccataac
     1681 ctgaactata cttatggagt gtctcagaaa cttgtgcacc ataccacatg attttcttag
     1741 gtacacaacc taagttaaca caagttccac ctacttgtcc accttcaatt aacaggactt
     1801 ttgctccata catggctgcc ctattagcgg taccactgcc tgcactacct ccaccaataa
     1861 cgatataatc atattgtttt gacattactc tctccttgaa tcattgatac ctatactcta
     1921 tcacaaagtg tttacagtca caattttctg ctacgtaaac aatttatatt accgattatc
     1981 atatcctaac ttgctgttaa aattatttct agtaacaata atctatcaat gcaatctttt
     2041 gctta
//
LOCUS       DBJYSH010000172         2006 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.172, whole genome shotgun sequence.
ACCESSION   DBJYSH010000172 DBJYSH010000000
VERSION     DBJYSH010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.172"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            42..167
                     /locus_tag="ACUBV3_001942"
     CDS             42..167
                     /locus_tag="ACUBV3_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3848411.1"
                     /translation="MRISEIADLLTSIGTLLVGIASIITAIKKEPKKKNRPRRFK"
     gene            303..473
                     /locus_tag="ACUBV3_001943"
     CDS             303..473
                     /locus_tag="ACUBV3_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3848412.1"
                     /translation="MDKELTPQEKANKKWAENNREHRTYLSKRSTARSFINKNATKED
                     LLELKQLIESKL"
     gene            complement(514..714)
                     /locus_tag="ACUBV3_001944"
     CDS             complement(514..714)
                     /locus_tag="ACUBV3_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HHU3848413.1"
                     /translation="MSQEKLKSKLDQAKGGAKEGFGKITGDKELEAKGFVEKTIAKGK
                     ELADDAKDAVEEAVDAVKEKLK"
     gene            complement(824..1123)
                     /locus_tag="ACUBV3_001945"
     CDS             complement(824..1123)
                     /locus_tag="ACUBV3_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS-like domain-containing protein"
                     /protein_id="HHU3848414.1"
                     /translation="MVTLTVKELAKNFSNDNKAGEILFEQLKSYFYTDTVVTVSFAGI
                     SEVSSSFVNSAFINLLSYYDFNHIKSQLKIVNSTKQINDLIKQRFSFEISRQITV"
     gene            complement(1117..>2006)
                     /locus_tag="ACUBV3_001946"
     CDS             complement(1117..>2006)
                     /locus_tag="ACUBV3_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HHU3848415.1"
                     /translation="LVLPQNLNKYNITKIVTNFNRLLALSDNRTLTVDMRNIEFAEPS
                     GVISLYNMLTFATKRKDANIKWLICEESSLNKRQRQAMLYLVDCGFFKVFDKLVYKEP
                     ELRPTTFEIKFINTEQIAQWKVTDFKNWLQKQTGRTNEFSSICVAVDEIFNNIADHSK
                     ESKGCIFGQYYPKNKEIVIAVSDFGIGIPQSIKRKFKKDEPDNKLIEFALQEGVSAET
                     IPQNRGAGLSNIVNTLTTNKVGNFTIISNCGIVSVSDNKITQSYSSEESYPGTFFEIR
                     IDVSNDNLYDLEEEEEFEW"
BASE COUNT          667 a          354 c          283 g          702 t
ORIGIN      
        1 atagtacatg tactataatt atatatagaa aggaggaaga tatgagaata tcagaaattg
       61 ctgatttgct tacttcaatc ggaactctgt tggttggtat agcaagcata atcacagcaa
      121 taaaaaaaga acctaaaaag aaaaaccggc cacggagatt caaataaggt tctagtagta
      181 gtttggggct caagcccctt gccactactg atagtatatc atatctaaga caaatatgaa
      241 atatttgatt attttcgcaa tttgtttagt tgtattttac tttattaaca aggatgattg
      301 aaatggataa agaattaaca cctcaagaaa aagcaaataa aaagtgggca gaaaacaata
      361 gagaacatag aacctatcta tcaaaacgat ctactgctcg tagttttatt aacaaaaatg
      421 ctacaaaaga agacttatta gaattaaaac aattaattga aagcaaactc tagacacaca
      481 aaaaaaccgc cctcaataga gagcggttaa tatttatttc agtttttctt tgacagcatc
      541 tactgcctct tcaacagcat ctttagcatc atctgctagt tctttgcctt tagcaattgt
      601 tttttcaaca aatccttttg cttctaactc tttatcaccg gttattttgc caaagccttc
      661 tttagcacca ccttttgctt gatctaattt tgattttagt ttttcttgtg acataatgtg
      721 cctccttatt atttttattc tatggtaaca ttttgattat ttctaggcaa ataaaaaagc
      781 aagaaccgct agtgtcaggc gattcttgct aaataatatg cacctatact gtaatctgcc
      841 tacttatttc aaaactaaat cgttgtttta ttaaatcatt tatttgtttt gttgaattta
      901 caatttttag ttgactttta atatgattaa aatcatagta agacaataaa ttgataaaag
      961 cagagtttac aaaggacgaa cttacttcgc taattcctgc gaacgaaaca gtcacgactg
     1021 tatctgtata aaaataagat ttcaattgtt caaataaaat ctctcccgcc ttattatcgt
     1081 tagaaaaatt ctttgctagt tctttgacag ttaacgttac cattcgaatt cttcctcctc
     1141 ttctaaatca tataaattgt cattcgatac atctattcga atttcaaaaa aagtaccagg
     1201 atatgattcc tcagacgaat aactttgagt aattttatta tctgataccg agactatacc
     1261 acaattagat ataattgtaa agttcccgac tttgttagta gttaaagtat ttacaatatt
     1321 agaaagccct gcccctctat tttgaggtat agtttctgca gaaacaccct cttgaagagc
     1381 gaattctatt aacttgttgt caggctcatc ctttttaaat tttcgcttta tagactgagg
     1441 gattcctatt ccgaaatcag atactgctat tacaatctcc ttgttcttgg gatagtattg
     1501 cccaaaaata catcccttag attccttaga atgatctgca atattattaa aaatttcgtc
     1561 aaccgctaca caaatagaac taaactcatt tgttctgcca gtttgctttt gtaaccaatt
     1621 cttaaagtct gttaccttcc attgagctat ttgttcagtg ttaataaatt taatttcaaa
     1681 agtagtcggg cgcagttccg gctctttata aaccaattta tcaaacactt taaaaaagcc
     1741 acaatctact agatacaaca tagcttgcct ttgacgttta tttaaagagc tttcttcgca
     1801 tattaaccac ttgatgtttg catcttttct ttttgtagca aaagttaaca tattatataa
     1861 cgaaattact ccactaggtt ccgcaaactc aatgtttctc atatctactg taagtgtcct
     1921 gttatcactt aaagcaagta atctattgaa attggtaacg attttcgtaa tgttatattt
     1981 gtttaaattc tgaggaagaa ctagat
//
LOCUS       DBJYSH010000173         1948 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.173, whole genome shotgun sequence.
ACCESSION   DBJYSH010000173 DBJYSH010000000
VERSION     DBJYSH010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1948
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.173"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            17..1372
                     /locus_tag="ACUBV3_001947"
     CDS             17..1372
                     /locus_tag="ACUBV3_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HHU3848416.1"
                     /translation="MKKESISIKDILWFIIPSLFGVLLLMTPFKYNGMTTVAVSVISK
                     TINQWINAVFPIHYIILLIIFISCVLALCYRLFRPSFIEKNDLLKEISDITIFWLIIR
                     LIGLALGLMTVLHIGPEMVWGKETGGLILFDLIGGLFTIFLAAGFILPFLTEFGLLEF
                     VGVFLTPIMRPFFQLPGRSAVNCVASFVGDGTIGIALTDKQYVEGYYTSREAATISTT
                     FSAVSITFCLVVLQNVGLTDYFGHFYLTVIISSVVCASIIPYIPPLSRQSNSYYTKPK
                     KETSEVIPDGYNKIEWGLKLAIDKSKANGNMKTFVNNASKTVLSLWFGVMPTIMTVGT
                     IALIISVSTPIFKILGTPFLPFLELLGIPEADIASQTMIVGFSDMVVPSIMAAEIHSE
                     MTRFIVATVSIVQLIYMSETGAVILGSKIPINILELFIIFIERTIISLPIIVLMAHLF
                     F"
     gene            1556..1690
                     /gene="rpmH"
                     /locus_tag="ACUBV3_001948"
     CDS             1556..1690
                     /gene="rpmH"
                     /locus_tag="ACUBV3_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HHU3848417.1"
                     /translation="MKRTYQPSKIRRQRKHGFRHRMSTKNGRRVLASRRRKGRKVLSA
                     "
BASE COUNT          574 a          307 c          370 g          697 t
ORIGIN      
        1 tatccaggag gattgtatga aaaaagaaag tatatctatt aaagatatct tgtggtttat
       61 tattccgtcg ttatttggag tattattatt aatgactcca tttaaatata atggtatgac
      121 aacagtagct gtctctgtaa tctctaagac aattaatcaa tggattaacg ctgtttttcc
      181 tatacattat attattttac taatcatttt tatttcatgt gtgttagcac tttgttatcg
      241 tctgtttaga cctagcttta ttgaaaaaaa tgacttgcta aaagaaattt ctgacattac
      301 cattttctgg ctgattattc gtttgatcgg tttggcactt ggtctgatga cggttttaca
      361 tattggtcct gaaatggtat gggggaaaga gacaggcggc cttatccttt ttgatttgat
      421 tggtggtttg tttaccattt tccttgctgc gggttttatt cttccatttt tgacagaatt
      481 tggtctactg gagtttgttg gtgtcttctt aacccccatt atgcgtccat tctttcagct
      541 acctggtcgg tcagcagtta attgtgtagc ctcatttgta ggtgatggta ctattggaat
      601 tgctttgacc gataagcagt atgttgaggg ttattatacc agtagagaag ctgcaacgat
      661 ttccactact ttttcagcag tatcaatcac tttttgtctt gttgttttgc agaatgttgg
      721 cttaactgat tattttggcc atttttatct aacagtcatc attagcagtg ttgtctgtgc
      781 atccattatc ccttatatcc ctcccttatc tcgtcaatca aatagttatt acactaaacc
      841 aaagaaagaa acatctgagg taattccgga tggttataac aagatagagt ggggacttaa
      901 gcttgccatc gataaatcaa aagcaaatgg aaacatgaag acttttgtca ataatgcctc
      961 taagacagta cttagtttat ggtttggcgt tatgccaaca attatgactg tcggaacaat
     1021 agctttgatt atttcagtat caacacctat ttttaaaatt ttaggtacac cattccttcc
     1081 attccttgaa cttttaggga ttccagaagc agatattgcg agtcaaacca tgattgttgg
     1141 tttttcagat atggttgttc cttcgataat ggcggctgaa attcacagtg agatgacaag
     1201 gtttattgtc gcaacagttt ctattgtgca attaatttat atgtctgaga ctggtgcggt
     1261 gatattaggt tccaagatcc ctatcaacat tctagaatta tttatcattt ttatagagag
     1321 aacaattatt tcattaccta ttattgtttt gatggcacat cttttctttt agttggagaa
     1381 aatagagaag catttcttgc taacaaatga aactatttac tgaaaaaaga caaatgaact
     1441 tgaaagtatt tcttgacaag aggcctaaaa ttgttataaa atagtatggt atgtttagta
     1501 actgatcaaa atagccggct aaacgaatac gaaaatctat caggaggtat tcaacgtgaa
     1561 acgtacttat caaccaagta aaatccgtcg ccaacgtaaa cacggattcc gtcaccgtat
     1621 gtcaactaaa aacggacgtc gcgtgcttgc tagccgccgt cgtaaaggac gtaaagtgtt
     1681 atcagcttaa tatagatagc aaaataatat caagccaaca ggtactcgag tctggtggct
     1741 tttttggtac cttcataacg ataaaggttt ttgtgagagt agtttgagta tattatagat
     1801 aaagttaatg aaaagtgagt ttataatgcg gaaattcgta gacaaatagc ccctattttt
     1861 gttaaaatat gaggagattt cttattgaga aatatcttta tagcgagtga tattttattt
     1921 tttagacgct tgtattattg aagtagtt
//
LOCUS       DBJYSH010000174         1940 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.174, whole genome shotgun sequence.
ACCESSION   DBJYSH010000174 DBJYSH010000000
VERSION     DBJYSH010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1940
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.174"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            <1..998
                     /locus_tag="ACUBV3_001949"
     CDS             <1..998
                     /locus_tag="ACUBV3_001949"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HHU3848418.1"
                     /translation="SRDTKVVLITNKDSLSKMTFRNKYDHNYIAVCILDSSEKDCYDL
                     KHNSLRIINKDALTSELTCLTVDQAFINIPIELFGKYQIQDIINDIEAMGVIVNVNVE
                     ALSFDNIGEKRIQTFEGYSVITYSMKFYKYSHLIAKRFLDITGAIIGLLICGIVAIFL
                     VPQIRKDGGPAIFSQNRVGRNGRIFRFYKFRSMRVDAEQIKKDLLVHNQMTGLMFKLE
                     DDPRITKIGKFIRKTSIDELPQFYNVLKGDMSLVGTRPPTVDEYEKYNSTQKRRLSFK
                     PGITGLWQISGRNNITDFDEIVKLDVQYINEWSIWSDIKIILLTLKVVLLGTGAK"
     gene            1022..1471
                     /gene="pssD"
                     /locus_tag="ACUBV3_001950"
     CDS             1022..1471
                     /gene="pssD"
                     /locus_tag="ACUBV3_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PssD/Cps14F family polysaccharide biosynthesis
                     glycosyltransferase"
                     /protein_id="HHU3848419.1"
                     /translation="MKICLVGSSGGHLAHLNLLKPILEKEDRFWVTFDKEDARSILRE
                     EIVYHCFFPTNRNVKNLVKNTILAFKVLRKERPDVIISSGAAVAVPFFYIGKLFGCKT
                     VYIEVFDRIDKPTLTGKLVYPVTDKFIVQWEEMKKVYPKAINLGGIF"
     gene            1471..>1940
                     /locus_tag="ACUBV3_001951"
     CDS             1471..>1940
                     /locus_tag="ACUBV3_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HHU3848420.1"
                     /translation="MIFVTVGTHEQQFNRLIKEVDRLKGTDAIDQEVFIQTGYSDFEP
                     QNCQWSKFLSYDDMNSYMKEAEIVITHGGPATFMNAVSKGKKTIVVPRQEQFGEHVNN
                     HQVDFVNKVKTMYNFDIVVDIERLQNVVYEGTMNRPFLETNRSNFIEEFKVILK"
BASE COUNT          680 a          251 c          387 g          622 t
ORIGIN      
        1 tttcacgaga taccaaagtt gttttgataa cgaataagga ttctttatca aaaatgacct
       61 ttaggaataa atacgaccat aattatatcg ctgtctgtat cttggattcc tctgaaaagg
      121 attgttatga tttgaaacat aactcgttaa ggataataaa caaagatgct cttacttcag
      181 agttaacctg cttaactgtt gatcaagctt ttattaacat acccattgaa ttatttggta
      241 aataccaaat acaagatatt attaatgaca ttgaagcaat gggagtgatt gtcaatgtta
      301 atgtagaggc acttagcttt gataatatag gagaaaagcg aatccaaact tttgaaggat
      361 atagtgttat tacatattct atgaaattct ataaatatag tcaccttata gcaaaacgat
      421 ttttggatat cacgggtgct attataggtt tgctcatatg tggcattgtg gcaatttttc
      481 tagttccaca aatcagaaaa gatggtggac cggctatctt ttctcaaaat agagtaggtc
      541 gtaatggtag gatttttaga ttctataaat tcagatcaat gcgagtagat gcagaacaaa
      601 ttaagaaaga tttattagtt cacaatcaaa tgacagggct aatgtttaag ttagaagatg
      661 atcctagaat tactaaaata ggaaaattta ttcgaaaaac aagcatagat gagttgcctc
      721 aattctataa tgttttaaaa ggtgatatga gtttagtagg aacacgccct cccacagttg
      781 atgaatatga aaagtataat tcaacgcaga agcgacgcct tagttttaag ccaggaatca
      841 ctggtttgtg gcaaatatct ggtagaaata atattactga ttttgatgaa atcgtaaagt
      901 tagatgttca atatatcaat gaatggtcta tttggtcaga tattaagatt attctcctaa
      961 cactaaaggt agtcttactt gggacaggtg ctaagtaaag gtaaggtttg aaaggaatat
     1021 aatgaaaatt tgtctggttg gttcaagtgg tggtcatcta gcacacttga accttttgaa
     1081 acccattttg gaaaaagaag ataggttttg ggtaaccttt gataaagaag atgctaggag
     1141 tattctaaga gaagagattg tatatcattg cttctttcca acaaaccgta atgtcaaaaa
     1201 cttggtaaaa aatactattc tagcttttaa ggtccttaga aaagaaagac cagatgttat
     1261 catatcatct ggtgccgctg tagcagtacc atttttttat attggtaagt tatttggttg
     1321 taagaccgtt tatatagagg ttttcgacag gatagataaa ccaactttga caggaaaatt
     1381 agtgtatcct gtaacagata aatttattgt tcagtgggaa gaaatgaaaa aagtttatcc
     1441 taaggcaatt aatttaggag gaatttttta atgatttttg tcacagtggg gacacatgaa
     1501 cagcagttca accgtcttat taaagaagtt gatagattaa aagggacaga tgctattgat
     1561 caagaagtgt tcattcaaac gggttactca gactttgaac ctcagaattg tcagtggtca
     1621 aaatttctct catatgatga tatgaactct tacatgaaag aagctgagat tgttatcaca
     1681 catggcggtc cagcgacgtt tatgaatgca gtttctaaag ggaaaaaaac tattgtggtt
     1741 cctagacaag aacagtttgg agagcatgtg aataatcatc aggtggattt tgttaataag
     1801 gtaaaaacaa tgtataattt tgatatcgtt gtagatattg aaaggttaca aaatgtagtc
     1861 tatgagggga cgatgaatcg tccgttttta gaaactaaca gaagtaattt tattgaagaa
     1921 tttaaggtaa tattaaagga
//
LOCUS       DBJYSH010000175         1928 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.175, whole genome shotgun sequence.
ACCESSION   DBJYSH010000175 DBJYSH010000000
VERSION     DBJYSH010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1928
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.175"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            115..243
                     /locus_tag="ACUBV3_001952"
     CDS             115..243
                     /locus_tag="ACUBV3_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HHU3848421.1"
                     /translation="MAFGDNGQRKKTGFEKLTLFVVILMVLVTVGGLVFGAISAIM"
     gene            304..1617
                     /gene="obgE"
                     /locus_tag="ACUBV3_001953"
     CDS             304..1617
                     /gene="obgE"
                     /locus_tag="ACUBV3_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HHU3848422.1"
                     /translation="MSMFLDTAKISVKAGRGGDGMVAFRREKYVPNGGPWGGDGGKGG
                     SVIFKVNEGLRTLMDFRYNRNFKAKAGEKGMTKGMHGRGAEDLIVSLPPGTTVRDANT
                     GKVITDLVEHDQEFVVARGGRGGRGNIRFATPRNPAPEIAENGEPGEERELQLELKIL
                     ADVGLVGFPSVGKSTLLSVVSAAKPKIGAYHFTTIVPNLGMVRTKSGDSFAMADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSASEGRDPYDDYISINNELETYNLRLME
                     RPQIIVANKMDMPDSEENLAAFKEKLAANYDEFDDMPMIFPISSLAHQGLENLMDATA
                     KLLANTEEFLLYDETDMQEDEAYYGFNEDERPFEITRDDDATWVLYGDKLEKLFVMTN
                     MERDESIMKFARQLRGMGVDEALRERGAKDGDIVRIGNFEFEFVD"
     gene            1643..1801
                     /locus_tag="ACUBV3_001954"
     CDS             1643..1801
                     /locus_tag="ACUBV3_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3848423.1"
                     /translation="MGDKPISFRDKDGNFVSAADVWNAEKLEELFNTLNPNRKLRLER
                     EKLAKEKG"
BASE COUNT          601 a          273 c          459 g          595 t
ORIGIN      
        1 atatggaagc gatttaataa agatatattt aagctaacgg tttgtagttt gcgaaataag
       61 acgtttcgtg ttaatataga cttaactata tattcagtaa atgaggtgac atgagtggct
      121 tttggagata acggacaacg aaaaaaaact gggtttgaaa aactaactct ttttgttgtt
      181 attttgatgg tactagtaac agttggtggt ttggtatttg gtgctattag tgcaatcatg
      241 taatgagaaa cgctgtaaag cgttttttta tactgaatag aataaagaat aggaatttta
      301 gaaatgagta tgtttttaga tactgccaaa attagtgtta aggcaggtcg cggtggtgat
      361 ggcatggttg cttttcgccg tgagaaatat gtccccaatg gaggcccgtg gggcggtgat
      421 ggcggtaaag gcggatcggt catttttaaa gtaaacgaag gtttacgaac tttgatggat
      481 tttcgatata accgtaattt taaagcaaaa gctggtgaaa aagggatgac taaaggtatg
      541 cacggtcgag gtgcagaaga ccttattgtg tcattaccac ctggaacaac tgttcgtgat
      601 gctaatacag gtaaggttat taccgacctt gtggagcatg atcaggaatt cgttgtagcc
      661 agaggtggcc gcggtggtcg tggaaatatt cgctttgcaa ccccacgtaa tccagctcct
      721 gaaattgctg aaaatgggga gcctggcgaa gaacgtgaat tacaattaga attaaaaatt
      781 ttggctgatg ttggtctcgt tggtttccca tcagttggga agtcgacgct acttagtgtt
      841 gtttcagcag caaagcctaa aattggtgcc tatcatttta cgacaattgt ccctaattta
      901 gggatggttc gtacaaaatc aggtgatagc tttgcaatgg ctgatctccc tggtttgatt
      961 gaaggggcta gtcaaggtgt cggattagga actcagttcc ttagacatat tgagcgtaca
     1021 cgtgttattc tacatgttat tgatatgtcg gcaagtgaag gacgagaccc ttacgatgac
     1081 tatatttcaa ttaataatga gttggaaact tacaatcttc gattgatgga acgtccgcaa
     1141 attattgttg ccaataaaat ggatatgcct gattcagaag agaatttggc agcattcaaa
     1201 gaaaaattag ctgcaaacta tgatgagttt gatgacatgc ctatgatttt tccaatttca
     1261 agtttagcac atcaaggact agagaacctt atggatgcga cggctaaatt attagccaat
     1321 acagaagagt ttcttcttta tgacgaaact gacatgcaag aagatgaagc atactatgga
     1381 tttaacgaag atgaacgtcc atttgagatt acacgtgatg atgatgcaac gtgggtattg
     1441 tatggtgata agctagaaaa actctttgtt atgacaaata tggaacgcga tgaatcaata
     1501 atgaaatttg ctcgtcagtt acgtggtatg ggcgttgatg aagcacttcg agagcgcgga
     1561 gctaaggatg gagatattgt tcgtataggt aacttcgaat ttgaatttgt agattaatgc
     1621 agtacttgat tggaggtttt ttatgggaga taaaccgata tcatttcgtg ataaagatgg
     1681 gaactttgtt tcagcggccg atgtctggaa tgctgaaaaa ttagaagaac tctttaacac
     1741 attaaatcct aatcgtaaac ttcgtttaga acgcgaaaaa ttagcaaaag aaaaaggata
     1801 gttaagtaac tatccttttt agttattttg ggtatgaaaa aagactctaa aacataattg
     1861 atagagtgtt aaggtacaga ttactttatt attttaaacg tttttgagct ttttgatata
     1921 ctgaagag
//
LOCUS       DBJYSH010000176         1927 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.176, whole genome shotgun sequence.
ACCESSION   DBJYSH010000176 DBJYSH010000000
VERSION     DBJYSH010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1927
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.176"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            complement(39..1136)
                     /locus_tag="ACUBV3_001955"
     CDS             complement(39..1136)
                     /locus_tag="ACUBV3_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HHU3848424.1"
                     /translation="MRIIAITEKVIKELFRDKRTLAMMFLAPILIMFLMNVMFSANSN
                     TKVKIGTINVNTKVVSNLDNIKHIQVRSFKFNSSAKKALKSNKIDALISEDNKSYTVF
                     YANTDSSKTTLTRQAFKTAVNTMNSKELISQVKILANKNPKLAQSLQTRSKYIKEKYN
                     YGNKNTGFFAKMIPILMGFMVFFFVFLISGMALLKERTSGTLDRLLATPVKRSDIVFG
                     YMLSYGILAIIQTVVIVLSTIWLLDIQVVGSIFSVIIVNFILALVALSLGILMSTLAK
                     SEFQMMQFIPLIIMPQLFFSGIIPLENMASWAQTVGKILPLSYSGDALTKIIMYGQGL
                     PNVSSNLLVLLLFLIILTIANIFGLKRYRKV"
     gene            complement(1139..1855)
                     /locus_tag="ACUBV3_001956"
     CDS             complement(1139..1855)
                     /locus_tag="ACUBV3_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HHU3848425.1"
                     /translation="MKKVIDLKKLQKAYASETVLNNINLEVFKGEIIGLIGPSGAGKS
                     TLIKTMLGMEKADKGTALVLDTQMPDRNILNQIGYMAQSDALYESLTALENLLFFGKM
                     KGIQKTELKQQITHISKVVDLENQLDKFVSGYSGGMKRRLSLAIALLGNPTVLILDEP
                     TVGIDPSLRRKIWQELINIKDEGRSIFITTHVMDEAELTSKVALLLRGNIIAFDTPLH
                     LKKQFNVSTIEEVFLKAEGE"
BASE COUNT          629 a          306 c          304 g          688 t
ORIGIN      
        1 aatattattt ttcatactta gttccttttc aaatggtatt atactttacg gtatcgtttt
       61 agtccaaaga tatttgcgat ggttaaaata attaaaaata gaagaagtac cagaagattt
      121 gaagaaacgt taggtaatcc ttgaccgtac ataattattt ttgtaagtgc atcaccagag
      181 taggatagcg gtagaatttt accgactgtc tgagcccaac ttgccatatt ttctagaggg
      241 attattcctg aaaagaataa ttgaggcatt ataattaagg gaataaattg catcatttga
      301 aattctgatt ttgctagagt agacattaag attcctaatg ataaggcaac cagagccaaa
      361 ataaaattga caatgatgac actaaaaata ctacctacaa cttgaatatc tagtagccaa
      421 atagttgata aaacgataac taccgtttga ataattgcta gaatgccgta tgataacata
      481 tatccaaata caatatcact acgtttaaca ggagttgcaa ggagtcgatc aagtgtacca
      541 gaagtacgct cttttaataa agccattcca gaaattaaaa aaacaaagaa gaagaccata
      601 aatcccatta gtattggtat catttttgca aaaaagcctg tatttttatt tccgtaatta
      661 tatttttctt tgatatattt ggagcgagtt tgtaaggatt gtgctagttt cggattctta
      721 ttagctaaaa ttttaacttg cgaaatcagt tccttactgt tcattgtatt aacagcggtt
      781 ttaaaagctt gtcttgttaa agtcgtcttt gaagaatctg tattcgcata gaagacagta
      841 taggatttat tgtcctccga aataagagca tcaattttat ttgatttgag tgctttttta
      901 gcagatgagt taaatttaaa tgatctcact tgaatatgct taatattatc taaatttgaa
      961 acgaccttcg tgttaacgtt aatagttcca atcttaactt ttgtattact attcgcagaa
     1021 aacataacat tcatcaaaaa cataattaaa ataggtgcta aaaacatcat agcaagtgtt
     1081 cttttatcac gaaacagttc ttttataacc ttttcagtta ttgcaataat tctcatgatt
     1141 attctccttc agcttttaag aaaacttcct caatagtact cacattaaat tgttttttta
     1201 aatgtaatgg agtatcaaag gcaataatgt ttccacgtaa tagtagtgca accttacttg
     1261 ttaattctgc ttcatccata acgtgggttg taataaagat agaacgtcct tcatccttaa
     1321 tattaattag ctcttgccag atttttctcc tcaaggatgg atcaattcca acggtaggtt
     1381 catctaggat taaaactgtg gggtttccaa gtagggcgat ggctagagaa agccgtcttt
     1441 tcatacctcc tgagtaacct gagacaaatt tatcaagttg gttttctaga tctactactt
     1501 tagaaatatg agttatctgc tgttttaatt cagttttttg aatacctttc atttttccaa
     1561 agaataataa attttctaag gcagttaaag attcgtataa ggcatcagat tgagccatat
     1621 agccaatttg atttaaaata ttacgatctg gcatttgagt atcaagaaca agagctgttc
     1681 ccttatctgc tttttccatg ccaagcatag ttttaatcaa ggtagatttc cctgctccag
     1741 agggtcctat taatccaatt atttcgcctt taaacacctc caaattaata ttatttaaaa
     1801 cagtttctga ggcatatgct ttttgtagtt tttttaaatc gatgactttt ttcatgataa
     1861 ctttttgctc ctctatcagt gagtgagtgt ttactcacct atttgatatc atatattata
     1921 gtgtgta
//
LOCUS       DBJYSH010000177         1927 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.177, whole genome shotgun sequence.
ACCESSION   DBJYSH010000177 DBJYSH010000000
VERSION     DBJYSH010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1927
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.177"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            complement(<1..1015)
                     /gene="trpS"
                     /locus_tag="ACUBV3_001957"
     CDS             complement(<1..1015)
                     /gene="trpS"
                     /locus_tag="ACUBV3_001957"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HHU3848426.1"
                     /translation="MTKPIILTGDRPTGKLHIGHYVGSLKNRVLLQNEGSYTLFVFLA
                     DQQALTDHAKDPQTIVESIGNVALDYLAVGLDPNKSTLFIQSQIPELAELSMYYMNLV
                     SLARLERNPTVKTEIAQKGFGESIPAGFLVYPVAQAADITAFKANLVPVGTDQKPMIE
                     QTREIVRSFNHAYNCQVLVEPEGIYPENDAAGRLPGLDGNAKMSKSLNNGIFLADDMD
                     TVKKKVMSMYTDPNHIKVEEPGQIEGNMVFHYLDVFGRDEDQKEITAMKEHYQKGGLG
                     DVKTKRYLLDILERELSPIRERRLEYAKDMGQVYQMLQKGSEKAQAVAASTLDEVKSA
                     MGLN"
     gene            complement(1123..1815)
                     /locus_tag="ACUBV3_001958"
     CDS             complement(1123..1815)
                     /locus_tag="ACUBV3_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001250491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HHU3848427.1"
                     /translation="MRRDDIIMPQDGRKMAVLLGFIGGFIDVYSHMQFHSLVATQTGN
                     IILMVANLGQNDFHFFITKLFSIFFFSIGFLVGIVIKERAVTAFWRLYALCPLLLSTM
                     FIPFVENVNHNIWVALLAFSTGILMLTFSGSRIESEPYTILMTSGNYRKMLQFWYEYI
                     VNRQRTAMQKRRAINYSLVVLAFIIGALVAAIVYDIFAYRAILGVTITLLIIMIHYTI
                     EIIKNDLTLHNV"
BASE COUNT          645 a          375 c          271 g          636 t
ORIGIN      
        1 agttcaaacc catggctgat ttaacctcat ccaaagtaga ggctgcaaca gcttgagctt
       61 tctcactacc tttttgtaac atttgataaa cttgtcccat atctttagca tattcgaggc
      121 gacgttctct aataggtgat aattcacgct ctaagatatc aagtagataa cgttttgttt
      181 taacatcccc aagtccacct ttttgatagt gttctttcat agcagtaatc tctttttggt
      241 cttcatctct tccaaaaaca tcaaggtaat gaaataccat attaccttcg atttgtcctg
      301 gttcttctac tttaatgtgg tttggatcag tatacatact cataactttt ttcttaactg
      361 tatccatatc atctgctaaa aagattccat tatttaatga cttagacatt tttgcattac
      421 catctaaccc tggcagacga cctgctgcat cattttctgg atagattccc tctggttcaa
      481 ctaaaacctg acaattataa gcatgattga aagatctaac tatttcacgt gtttgttcaa
      541 tcattggttt ttggtcagtc cctacaggaa caaggttagc tttaaaagcg gttatatcag
      601 ctgcttgtgc aacaggataa actaagaatc ctgctggaat agactcacca aaaccttttt
      661 gagcaatttc tgtttttaca gttggattac gttctaaacg tgcaagtgaa accaagttca
      721 tataatacat acttaattct gctaactcag gaatttgact ttgaatgaat aaagtcgact
      781 tgtttggatc taacccaaca gctaaataat caagtgcaac attaccaatt gattcaacaa
      841 tagtttgcgg atctttagca tggtctgtca gtgcttgttg gtctgctaaa aagacaaata
      901 aagtatagct accctcattt tgaagtagaa ctctattttt taagctacct acataatgtc
      961 caatatgcaa ctttcctgtc ggtctatcac ctgttaagat aattggtttt gtcatgttta
     1021 ctccttaata taccaaagat atcatttcat atagttatct tatcatgtca caagccctat
     1081 tgcaattcat ccctacaaga aacattcacc ttattaagat gattaaacat tatgtaacgt
     1141 taaatcgttc ttaataattt caatcgtata atgaatcata ataattaaaa gcgttatcgt
     1201 cacacctaaa atagctctat atgcaaaaat atcgtaaact attgcagcta ccaatgctcc
     1261 aataataaat gctagaacta ccagtgaata attgatagct cgtctttttt gcatagccgt
     1321 tctctgacga ttaacaatat actcatacca aaattgtagc attttccgat aattaccaga
     1381 agtcattaag atagtatatg gttccgattc aatgcgactt cctgaaaatg tcaacattaa
     1441 aatacctgtg ctaaaggcta ataaagctac ccagatattg tggtttacat tttcaacaaa
     1501 aggaataaac atcgtagaaa gtaaaagggg acataatgca tatagacgcc aaaaagctgt
     1561 tactgcacgt tctttaatca caataccaac caaaaaacca attgaaaaaa agaaaataga
     1621 aaataattta gtgataaaaa agtgaaaatc attttgtccg agatttgcca ccatcaagat
     1681 aatattacct gtctgtgttg caacaaggga gtgaaactgc atgtgactat aaacgtcaat
     1741 aaaaccacca ataaaaccca ataatacagc catttttctt ccatcttgtg gcattataat
     1801 atcatctctg cgcattatag caaattctcc ttcttgtatt atcatgtttt ctattatata
     1861 ccaataaaat aggcattcaa ctcaaaaata tcgagaaatt tcttttctaa atttacttga
     1921 ctaccaa
//
LOCUS       DBJYSH010000178         1906 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.178, whole genome shotgun sequence.
ACCESSION   DBJYSH010000178 DBJYSH010000000
VERSION     DBJYSH010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1906
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.178"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            9..>1906
                     /gene="srr2"
                     /locus_tag="ACUBV3_001959"
     CDS             9..>1906
                     /gene="srr2"
                     /locus_tag="ACUBV3_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086874280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-rich repeat glycoprotein adhesin Srr2"
                     /protein_id="HHU3848428.1"
                     /translation="MLKKQFGNFGEKSRKVRVKMRKSGKHWVKSVMTQIGYVILSRFS
                     GKEKSSKVQTTSEDLSRTKTSASILTAVAALGAVVGGTTDTTSVSAEETPTATELTGN
                     EKTLATAETVVVAPEVKTVNSDSSSHSTSESQSMSTSTLQSTSASLSASESLMDSTSA
                     SLSESSSLSEYSSLSLSSSESVSASESVQSSEAATTARVQPRAMRVVSSASDMETLPA
                     ALISGEGDVTTVQGQDVTDKLQNLDIKLSGGVQAKAGVINMDKSESMHMSLKFTIDSV
                     NRGDTFEIKLSDNIDTNGASNYSIVEPIKSPTGEVYATGIYDSQKKSIVYSFTDFAAS
                     KNNINGILDIPLWPDDTTVQNTKEDVLFSVKIKDQEATIKETVKYDPPVRIDFAGGVS
                     VDSRITNIDDVGKKMTYISQINVDGKSLYNYNGLYTRIYNYSKESTADLKNSTIKIYK
                     TTSDNIVESMVQDYSSMEDVTSKFANSYPEKGWYDIYWGQFIASNETYVIVVETPFTN
                     AVTLNTTLSDYNENNGVEHNHTYSSESGYSDVNAQERKILSELVSSSESVSSSESVSN
                     SESISTSESVSNSESISSSESVSSSESISTSESVSTSESISSSESVSSSESVSSSESI
                     SSSESVSNS"
BASE COUNT          681 a          311 c          369 g          545 t
ORIGIN      
        1 gaggaaaaat gttaaaaaag cagtttggaa actttggtga aaaatcacgc aaagttcgag
       61 ttaaaatgag gaaatcaggt aagcactggg tcaaaagtgt tatgacacaa attggctatg
      121 ttatcctttc gaggtttagc ggtaaagaga aaagctctaa ggttcaaact actagtgaag
      181 atttaagcag aactaaaacg tctgctagca tcctaacagc agtcgcagct cttggagctg
      241 tagttggagg gacgacagac acaacttctg tctcagctga agaaacacct actgcaacag
      301 aacttacagg aaatgaaaaa acattagcta ctgctgaaac tgtagtggtt gcgccagaag
      361 taaaaactgt aaactcagat tcttcatcac attcaacaag tgaatcacaa tcaatgtcaa
      421 ctagtacctt gcaatctacc agcgcctcat tatctgccag tgaatcacta atggattcta
      481 cctctgcttc tttatctgag tctagctcgt tatcagaata tagttcttta tctctatcta
      541 gtagtgagag tgtctcagca tcagaatcag ttcaatcatc agaagcggca acgaccgcta
      601 gagttcaacc gagagcgatg agagttgtat ctagtgcttc agatatggaa actttaccag
      661 cagcattaat ctctggtgaa ggagatgtaa caactgttca aggacaagat gtaacagata
      721 agttacaaaa tttagatata aagctctctg gaggagtgca agcaaaagca ggtgttataa
      781 atatggataa gtcagaaagt atgcacatgt ctttaaaatt cactattgat tctgtgaata
      841 gaggtgatac ttttgaaatt aagttatcag ataatatcga tacaaatgga gcttctaact
      901 attctattgt agaacctata aaatcaccta cgggtgaggt ctatgcgact ggaatttatg
      961 attctcaaaa aaaatctata gtctatagtt ttacagattt tgcggcttca aaaaataata
     1021 ttaatggaat attagatatt ccattatggc cagatgatac gacagttcaa aacacaaaag
     1081 aagatgttct tttttcggta aaaataaagg atcaagaggc tacaattaaa gaaacagtga
     1141 aatatgatcc accggtaaga attgattttg cagggggagt aagtgtagat tctcgaataa
     1201 ccaacattga tgatgtggga aaaaagatga cttatataag tcaaattaat gtagatggaa
     1261 aatcactcta taattacaac gggttatata ctaggatata taattatagc aaagagagta
     1321 cagcagattt aaagaattca acgataaaaa tctataaaac cacctcggat aatattgtag
     1381 agagtatggt acaggattat tcaagcatgg aggatgtaac tagtaagttt gcaaatagtt
     1441 acccagaaaa agggtggtat gatatttatt gggggcagtt tattgcatca aatgaaacgt
     1501 atgtaattgt tgtagaaaca ccatttacta atgcagtaac tttgaatact actttatcag
     1561 attataatga gaacaatggt gtagaacata atcatactta ctcatctgaa tcgggatatt
     1621 cagatgtaaa tgctcaagaa agaaaaattt tatccgaatt agtaagtagc tcagaatcag
     1681 tatcaagttc agaatcggta tctaattccg aatctatttc aacttctgaa tcggtatcta
     1741 actccgagtc tatttcaagt tccgaatcgg tatcaagctc agaatctatt tcaacttctg
     1801 aatcggtatc aacttccgag tctatttcaa gctccgagtc agtatcaagt tcagaatcgg
     1861 tatcaagctc agaatctatt tcaagttccg aatcggtatc taactc
//
LOCUS       DBJYSH010000179         1880 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.179, whole genome shotgun sequence.
ACCESSION   DBJYSH010000179 DBJYSH010000000
VERSION     DBJYSH010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1880
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.179"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            <1..1241
                     /locus_tag="ACUBV3_001960"
     CDS             <1..1241
                     /locus_tag="ACUBV3_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HHU3848429.1"
                     /translation="VLQDFDNLLKKYAQLIISKGLNVQKGHTLALTIDVEQVHLARLL
                     TEVAYEKGASEVIVDYTDDFITRQRLLHASDEVLTNVPQYTVDKSLALLNKKASRLVV
                     KSSNPNAFATVDPKRLSKTTRATAIALEEQSRAIQANKVSWNVAAAAGREWAALVFPE
                     LKTSDQQVDALWDTIFKLNRIYEDNPIAAWDAHEAKLLEKATRLNQEQFDALHYTAPG
                     TDLTLGMPKNHIWEAAGSLNAQGETFIANMPTEEIFSAPDYRRADGYVTSTKPLSYAG
                     VIIENMTFTFKDGKIINVTAEKGQETIQRLIEENDGARSLGEVALVPHKTPISLSGLI
                     FFNTLFDENASNHLAIGSAYAFNVEGGTEMTSQELDEAGLNRSSTHVDFMIGSEQMDI
                     DGIRADGTAVPIFRNGEWAI"
     gene            complement(1275..1853)
                     /locus_tag="ACUBV3_001961"
     CDS             complement(1275..1853)
                     /locus_tag="ACUBV3_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HHU3848430.1"
                     /translation="MKVKNKILTMVALTVLTCATYSSIGYADTSDKNTDTSVVTTTLS
                     EEKRSDELDQSSTGSSSENESSSSSEPETNPSTNPPTTEPSQPSPSEENKPDGSTKTE
                     IGNNKDISSGTKVLISEDSIKNFSKASSDQEEVDRDESSSSKANDEKKGHSKPKKELP
                     KTGDSHSDTVIASTGGIILLSLSLYNKKMKLY"
BASE COUNT          564 a          350 c          351 g          615 t
ORIGIN      
        1 tggttttaca agatttcgac aaccttttaa aaaaatatgc ccaattaatt atttctaaag
       61 gtttaaatgt ccaaaaaggg cacactctcg ctttaacaat cgatgtggaa caagtccact
      121 tagcaaggct tttaactgaa gtcgcttatg aaaagggagc aagtgaagtt attgttgatt
      181 atacagatga ttttatcacg cgccagcgac tacttcatgc ttcagacgaa gttctcacga
      241 atgttccaca gtataccgtt gataaatctt tagcgctatt aaataagaag gctagtcgat
      301 tagttgtgaa atcttctaac cctaacgctt tcgctactgt tgatcctaaa cgtttatcta
      361 aaacaactag agcaaccgct attgccttag aggaacaaag tagagcaata caagctaata
      421 aagtatcttg gaacgtggct gcagctgctg gtagagaatg ggctgcactt gtcttcccag
      481 aattaaaaac aagcgaccaa caagttgatg ctctttggga taccattttc aaattaaatc
      541 gtatttatga agataatcct attgctgctt gggatgcaca tgaagctaaa ttattagaaa
      601 aagctactag actaaatcaa gaacaatttg atgctcttca ttataccgca ccaggtacag
      661 atttaacgct tggtatgcct aaaaatcaca tttgggaggc agccggtagt ctcaacgctc
      721 agggagagac ttttatcgct aatatgccta ctgaagaaat cttttcagca cctgattacc
      781 gtcgtgcaga tgggtatgtg acaagtacaa aacctctcag ttatgctggc gttattatcg
      841 aaaatatgac gtttactttt aaagacggta aaattatcaa tgtcactgca gaaaaagggc
      901 aagaaacaat ccaacgcttg atcgaggaaa atgatggggc aagatcgctt ggggaagttg
      961 cacttgtccc acataaaaca ccaatttcac tgtctggact aattttcttt aatactttat
     1021 tcgatgaaaa tgcctctaat cacctcgcta ttggatctgc atatgccttc aatgtagaag
     1081 gaggaacaga aatgacaagt caagaattgg atgaagctgg tttaaatcgt tcttcaacac
     1141 atgttgattt tatgattggt tcagaacaaa tggatattga tggtattcgt gcagatggaa
     1201 ctgctgtccc aatctttaga aatggcgaat gggctattta aatataaaaa ggaggttagt
     1261 cctccttttt atatttaata aagtttcatt ttcttattgt aaagacttaa tgataacaga
     1321 ataatccctc ccgtagatgc tattacagta tctgagtggc tatctcctgt tttaggaagt
     1381 tcctttttag gcttactgtg gccttttttc tcatcatttg cttttgaaga tgatgattca
     1441 tcgcgatcca cttcttcttg atcactactt gctttactaa aattcttaat actatcttct
     1501 gaaattaata cttttgttcc actagaaata tccttattat tgccaatttc tgtcttcgtg
     1561 ctcccatcag gcttgttctc ttcactaggt gagggttgcg atggttctgt tgtaggtgga
     1621 ttagttgagg gatttgtttc tggttcactt gatgaacttg attcattttc agaagaagaa
     1681 ccagtactag actggtctag ttcatctgat cttttctcct cagataaggt cgtagtcacg
     1741 acactcgtgt cagtattctt atcacttgta tcagcataac cgattgatga ataagtagca
     1801 catgttaaga cagtaagtgc taccatcgtt aaaatcttat ttttcacttt catattattt
     1861 cctattttat aattccttac
//
LOCUS       DBJYSH010000180         1861 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.180, whole genome shotgun sequence.
ACCESSION   DBJYSH010000180 DBJYSH010000000
VERSION     DBJYSH010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1861
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.180"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            complement(<1..227)
                     /locus_tag="ACUBV3_001962"
     CDS             complement(<1..227)
                     /locus_tag="ACUBV3_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1361 domain-containing protein"
                     /protein_id="HHU3848431.1"
                     /translation="MKVKLFAIHLFFIVISFGIKYYHLQGPNLIWNMTLALIALDFAY
                     LTSLFKKKILIGLFALAWFFFYPNTFYMLTD"
     gene            complement(317..901)
                     /locus_tag="ACUBV3_001963"
     CDS             complement(317..901)
                     /locus_tag="ACUBV3_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000734041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3848432.1"
                     /translation="MKKLIWSHLFAIVSILGIWYYHVKIDHLMVILLASVLSLEMGFI
                     AYYSRNIFIRLLASLGLLFFYPQSLGIFLSLVSLEWSTKDIWQSNHFMQYMAYLLAIL
                     FIISSALLSLKLLVNAYDISKRYQLATVIPVMLFSSFAFCLEKSGSLGLEGIILNPSA
                     FFNGIQTNMANLDFKALSCLFLVQLLLWGILMED"
     gene            990..1802
                     /locus_tag="ACUBV3_001964"
     CDS             990..1802
                     /locus_tag="ACUBV3_001964"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HHU3848433.1"
                     /translation="MTKKIIAVDLDGTLLHNNNTISDYTADTLRKVQAQGHKVIITTG
                     RPYRMALAHYLRLDLKTPMINFNGALTHIPEKKWAFEHSATIDKKLLLETLNLSDAIQ
                     ADFIASEYRKNFYITMDNRDKINPQLFGVNEITDKMALDVTKITRNPNALLMQTRHKD
                     KYELAKELRQHFNHELEVDSWGGPLNILEFSPKGVNKAYALKHLLKSLNLSQENLIAF
                     GDEHNDTEMLAFAHTGYAMKNASPTLLPYADQQIQWTNEEDGVAKTLEKLLL"
BASE COUNT          718 a          334 c          255 g          554 t
ORIGIN      
        1 atatccgtca acatataaaa ggtattagga tagaaaaaaa accaagcaag tgcaaaaaga
       61 ccgataagaa tttttttctt aaataaacta gtaaggtatg caaaatctaa cgctattagg
      121 gctagagtca tattccaaat caaatttggt ccctgcaaat gataatattt aatcccaaaa
      181 gaaataacaa taaaaaataa atgtatcgca aatagtttta ctttcattct aacctccaat
      241 taatatctca ttatacaaca tattattaaa ttaaggttaa ataaacttca aacctaaaag
      301 actaaccctg ttgtggttag tcttccatta atattcccca aagtaataat tgtactaaaa
      361 aaaggcaaga caaggcttta aaatcaaggt tagccatatt agtctgtatt ccattaaaaa
      421 atgctgacgg atttaaaatg attccttcta aacctaatga tcctgatttt tctaagcaaa
      481 aggcaaaact actaaaaagc attacaggta taacagttgc taattgatac cttttactaa
      541 tatcatatgc atttaccaat agcttcaaac ttagtaaagc tgaagagata ataaaaagaa
      601 ttgcaagaag gtatgccata tattgcataa agtgattaga ctgccaaatg tcttttgttg
      661 accactctaa gctcactaaa ctaagaaaaa tccctaaact ctgtgggtaa aaaaataaaa
      721 ggcctaaact tgctaaaaga cgaataaaaa tattacgact ataataagca atgaatccca
      781 tctctaacga caagactgat gccaatagaa ttaccatcaa atgatcaatt tttacatgat
      841 aataccatat ccctaaaata ctgactatgg caaaaagatg actccaaatt aactttttca
      901 taatccacca acttccatat ttccacagtt attatatcat atttctactg tctcttttta
      961 ttgaaatcta tttttgatat aatgtcagca tgacaaagaa aattatcgcc gtagatttag
     1021 acggtactct attacataat aataacacta tttctgacta caccgctgat acacttcgaa
     1081 aagttcaagc tcaaggacac aaagtaatta ttacaaccgg acgcccatac cgtatggcac
     1141 tcgctcatta tttacgccta gatttaaaaa caccaatgat aaattttaac ggtgctttaa
     1201 ctcatatccc cgaaaaaaag tgggcgtttg agcatagtgc aacgattgat aaaaaattac
     1261 tccttgaaac cttaaattta tcagatgcta tccaagccga ttttattgct agcgagtacc
     1321 gtaaaaattt ttacattact atggataatc gtgataaaat taacccacaa ttattcggtg
     1381 tcaatgaaat tactgataaa atggcattag atgttactaa aatcacacgc aatccaaatg
     1441 ctctattgat gcaaacacgt cataaagata agtatgagtt agctaaagag ttacgacaac
     1501 attttaatca cgaactagaa gtagattctt ggggtggtcc attgaatata ctcgaatttt
     1561 cacctaaagg tgttaacaaa gcttatgctc tcaaacattt attaaaatca ctcaatcttt
     1621 cacaagaaaa tttgattgcc tttggagatg aacacaatga caccgagatg ctggcctttg
     1681 cacatacagg atatgctatg aaaaatgcta gtccaactct acttccatat gccgaccaac
     1741 aaatccaatg gacaaacgaa gaagatggtg ttgctaaaac tctagaaaaa ttattattgt
     1801 agtattttca aaaacgatct ctattgaaac agaattctaa tagaaatcgt tttttaggtt
     1861 t
//
LOCUS       DBJYSH010000181         1853 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.181, whole genome shotgun sequence.
ACCESSION   DBJYSH010000181 DBJYSH010000000
VERSION     DBJYSH010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1853
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.181"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            12..>1853
                     /locus_tag="ACUBV3_001965"
     CDS             12..>1853
                     /locus_tag="ACUBV3_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006740417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin-like domain-containing protein"
                     /protein_id="HHU3848434.1"
                     /translation="MFSKARGTQYDASQTKQRFSIKKFKFGAASVLVGLLFLGMNSHS
                     VLADESSAMSAEVTQAAEVTSVDNTINSDTVVSAGHTENINPDDVVKISESGISEDSN
                     ISSEDTDKVNDLGETVSEKDITVPPVNTMRSIDDADESLEKASVDNNRGSEVSGEHQN
                     KEEQDTVYTLQDTPNKQEYSDKYQLPSRDSVDKPKVRSRRSLNLSDRESSSQEFPLGS
                     AEYVDKAHLIVTKNNMVDHFEVRGTAHGDDQNPVVLTEDVSGQAGSLLLNKRIDMNES
                     FRLEGRLSLGDKYEGYNVGGRSGGDGVSFAFTSTAPGTIGLSGASIGLGGLPNSFGFK
                     FDTWHNTSNPNVNQKAGADPKFSGYQNGAFGAFYGTDASGKAITLLQDARRLESQPHN
                     NEFKNVIVDYNGQSKAMTVTYDGQIFTKNIQTYLDKSRLTTNQAKGREELGFAIFAST
                     GSGTNLQQFDLTRFEYSSGGSYIKLLYVDDSTGNVIKEKLYSGLTYQSLDLSDKLMLP
                     NYTFKRTNAPTAVGYQDSNTINFNSGIQTITYTYKKVEKEELQRLLDNDSNLKLTDIY
                     VNADKTKQTAYDDSIKKGQVVVNDSFTSQDKVDDAVKIITDSIVALRV"
BASE COUNT          655 a          254 c          395 g          549 t
ORIGIN      
        1 ggagactacg tatgtttagt aaggcaagag ggacacaata cgatgcttca cagacgaaac
       61 aacgcttttc gattaagaag tttaagtttg gtgctgcttc tgtattagtc gggttattat
      121 ttttaggaat gaatagtcat tctgttttgg cggatgagtc aagtgctatg agtgcagagg
      181 taacacaagc ggctgaagta acctcagtgg acaatacaat taattcagat actgtagttt
      241 cagcaggtca tacagaaaat ataaatcctg atgatgtggt taaaatttcg gaatctggta
      301 tcagtgaaga ctctaatatc tcatcagaag atacagataa agttaatgat ttgggagaaa
      361 ctgtaagtga aaaagatatt actgtacctc ctgtaaatac aatgcgttct atagatgatg
      421 ctgatgaaag tttggagaaa gctagtgttg ataataatag gggaagcgaa gtatctggtg
      481 aacatcaaaa taaggaagaa caggatacgg tatataccct tcaggatact ccaaacaagc
      541 aggagtactc agataaatat cagttacctt caagagatag tgtagataaa ccaaaagtaa
      601 ggtcacgtcg ttcattaaat ttatctgata gagaatctag tagtcaagag tttccattgg
      661 gatctgctga gtatgtggat aaagctcatc taattgtcac taaaaataat atggttgacc
      721 actttgaagt tagaggaaca gcacatggtg atgaccaaaa tcctgttgta ctaactgaag
      781 atgtttcagg tcaagctgga agtttacttt tgaataaaag aattgatatg aatgaatctt
      841 ttagactaga aggtagatta agtttaggtg ataaatatga aggatacaac gttggtggaa
      901 gaagcggtgg agatggagtt tcttttgctt ttacttcaac tgcaccaggg acaattggac
      961 tatcaggagc gagtattgga ttaggtggcc ttccaaattc ttttgggttt aagttcgaca
     1021 catggcataa tacctcaaac cctaatgtaa atcagaaagc aggagcggat cctaagttta
     1081 gtggatatca aaatggggct tttggtgcct tttatggcac agatgctagt ggaaaagcaa
     1141 ttacattgtt gcaagatgct cgaagattag agtcacaacc gcataataac gaatttaaaa
     1201 atgtgattgt tgattataat ggacaatcaa aggctatgac agtgacatat gatggacaaa
     1261 tattcacaaa aaatattcaa acctatttag ataaaagtag actaactact aaccaggcta
     1321 agggaagaga agagttgggc tttgcgattt ttgcttctac tgggtcagga acaaatttac
     1381 aacaatttga tttaactagg tttgagtatt cttcaggtgg tagctatata aaattattgt
     1441 atgttgatga ttcaactgga aatgttatta aagaaaagct atactcaggt ctgacttatc
     1501 aatctctaga tttgtcggat aaattgatgt tacctaatta tacatttaag aggactaatg
     1561 ctcctactgc agtaggatat caggacagta acacaattaa tttcaattca ggtattcaaa
     1621 ctattaccta tacttataaa aaggttgaga aagaagaatt acaacgactt ttagataatg
     1681 attctaattt gaagttaact gatatatatg ttaatgcaga taaaacaaaa caaacggctt
     1741 acgatgattc tatcaaaaaa ggtcaagtag tagtaaacga tagttttaca agccaagata
     1801 aagtagatga tgcagttaag attataactg atagtatagt tgctctaaga gtt
//
LOCUS       DBJYSH010000182         1689 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.182, whole genome shotgun sequence.
ACCESSION   DBJYSH010000182 DBJYSH010000000
VERSION     DBJYSH010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1689
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.182"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            367..1644
                     /gene="ltrA"
                     /locus_tag="ACUBV3_001966"
     CDS             367..1644
                     /gene="ltrA"
                     /locus_tag="ACUBV3_001966"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001901474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HHU3848435.1"
                     /translation="MSELLDKISSRNNMLEAYKQVKSNKGSAGIDGVTIEQMDDYLHQ
                     NWRETKKLIKERSYKPQPVLRVEIPKPNGGVRNLGIPTAMDRMIQQAIVQVLSPLCEK
                     HFSEYSYGFRPNRSCETAIVQLLEYLNDGYEWIVDIDLEKFFDTVPQDRLMSLVHNII
                     QDGDTESLIRKYFHSGVVINGQRHKTLVGTPQGGNLSPLLSNIMLNELDKGLEKRGLR
                     FVRYADDCVITVGSEAAAKRVMHSVSSYIEKRLGLKVNMTKTKIVRPNKLKYLGFGFW
                     KSPKGWKCRPHQDSVQSFKRKLKQLTMRKWSIDLITRIERLNWVIRGWINYFSLGNMK
                     SIMTQIDERLRTRIRVIIWKQWKKKAKRLWGLLKLGVARWIADKVSGWGDHYQLVAQK
                     SVLKRAISKPALAKRGLVSCLDYYLERHALKVS"
BASE COUNT          538 a          329 c          416 g          406 t
ORIGIN      
        1 gatatgaagg cgtatatagc ggatgagggg gctataaact ctaaagtcca aataggtagt
       61 cgtaacctat atacgtaaat cacgagagta aacgaggaaa gatgtccgac ttatcccgtg
      121 aggtcctgtc aagggtaaaa cctagtaaca acgaacgaca agaagtcagc cgaacccata
      181 gtagtgatga agtttctgta atggaaatgg aacgaagggg tgaacaacta atcgaagtaa
      241 tcctacttca cttgtgtctg taaaacaaac ggtctgatag aactagatgc ggttacgtat
      301 tgactagagg aaggttcacc aatgtaagat atcatgcaga caacgaaaaa agaaaggaat
      361 acgcacatgt cagaattgct agataagata tcatctcgga acaatatgct cgaagcttac
      421 aagcaagtga aatcaaacaa aggttctgct ggtatcgatg gggtcactat cgagcagatg
      481 gatgactatc ttcaccaaaa ttggcgagaa accaagaaac tcatcaaaga gaggagctat
      541 aaacctcaac cggttctcag ggttgaaatc ccaaaaccaa acggaggagt tcgtaaccta
      601 ggtatcccga cggctatgga tagaatgatt cagcaggcca tcgttcaagt tttgagtcca
      661 ctctgcgaaa aacatttttc agagtatagc tatgggttca gacccaatcg ctcctgcgaa
      721 acagccattg ttcagctact tgagtattta aacgatggct acgagtggat tgtggacatt
      781 gacttggaaa agttcttcga tactgttccg caagacagat tgatgtccct ggttcataat
      841 atcattcaag atggcgatac ggagtcactg attcgtaagt acttccattc gggagttgtt
      901 attaacggac agcgacataa gactttagtc gggacacctc aaggcgggaa tctatcaccc
      961 ctcctatcta atattatgct taatgagtta gacaaagggt tggaaaagcg aggtcttcgc
     1021 tttgtccgtt acgccgatga ctgtgtcatc actgtcggaa gcgaagcagc tgctaagcgg
     1081 gtcatgcatt cggtcagtag ctatattgag aagcgattag ggttgaaagt caacatgact
     1141 aagaccaaga ttgtcagacc gaacaaactc aaatacctcg gatttggttt ctggaaatct
     1201 ccaaaaggtt ggaagtgtcg tcctcaccaa gacagcgttc agagctttaa gcgaaaactg
     1261 aagcaactga cgatgaggaa atggagcatt gacctgataa ctcgcattga acgattgaac
     1321 tgggtcattc gaggatggat aaactatttc tcgcttggca atatgaagag tatcatgaca
     1381 caaatagatg agcgtctgcg aacccgtatt cgagtgatta tctggaagca atggaagaag
     1441 aaagcaaagc gcctatgggg actcttaaaa ctaggagttg ctagatggat agccgataaa
     1501 gtttctggat ggggtgacca ctatcagttg gtagctcaga agtcggtact caaacgtgct
     1561 atatcaaaac cagccctcgc aaagcgagga ctggtcagtt gcttagatta ctatcttgaa
     1621 cgacatgcgt taaaagttag ttgaaccgcc gtatgccgaa cggcacgtac ggtggtgtga
     1681 gaggggcta
//
LOCUS       DBJYSH010000183         1641 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.183, whole genome shotgun sequence.
ACCESSION   DBJYSH010000183 DBJYSH010000000
VERSION     DBJYSH010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1641
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.183"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            <1..773
                     /locus_tag="ACUBV3_001967"
     CDS             <1..773
                     /locus_tag="ACUBV3_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HHU3848436.1"
                     /translation="LSVVTLVACGSSDKQLQDKVEKKGKLVLAVSPDYAPFEFKALVN
                     GKDKVVGADIELAQAIADELHVKLEVSPMSFDNVLSSLQTGKADMAISGLSYTKERAK
                     VYDFSKPYYTTENAVLVRKSDLGKYTSIESLKGKKIAVQKGSIEEGVSKKSFKNSHVV
                     SLTAMGEAISELKSGKVEAVDLEKPVAEGYLAQNTDLVLAKFALKTGEGDAKAVALPK
                     DSGKMLKTVNKVIKRLEKEDKYKVYISDAAKLTGNQVE"
     gene            856..1359
                     /locus_tag="ACUBV3_001968"
     CDS             856..1359
                     /locus_tag="ACUBV3_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HHU3848437.1"
                     /translation="MVQGSINIEKYKDLALQKQGEHRKFLASLKKKAPKNLDKIVQQI
                     HQEVFQEIDCTACANCCKTLGPLFTEADISRISKHFRMKLSAFEDLFLEVDEDGDKVF
                     KAMPCPFLGDDNLCSIYDVRPKACREFPHTDRKKIYQINNLTIKNTLTCQAAYLFVEK
                     LKSRIGK"
     gene            1463..1639
                     /gene="rpsU"
                     /locus_tag="ACUBV3_001969"
     CDS             1463..1639
                     /gene="rpsU"
                     /locus_tag="ACUBV3_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HHU3848438.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVTKAGTLQESRKREFYEKPSVK
                     RKRKSEAARKRKKF"
BASE COUNT          589 a          235 c          333 g          484 t
ORIGIN      
        1 ttttatctgt cgttactttg gtggcgtgtg gttcatcaga caaacagcta caagataaag
       61 ttgagaaaaa agggaagtta gttttagcgg tgagtccaga ttatgctccc tttgagttta
      121 aagcgcttgt taatgggaaa gataaagttg tcggcgcaga tattgagtta gctcaagcta
      181 ttgctgatga gcttcatgtc aaactagagg tatctcctat gagttttgac aatgttttat
      241 caagtttaca aacaggtaag gctgatatgg ctatttctgg attatcatat actaaagagc
      301 gtgctaaagt ctatgatttt tcaaaacctt attacactac agaaaatgcg gttcttgtta
      361 ggaagtctga tttaggaaaa tatacttcaa tcgaaagcct taaaggtaaa aaaatagcag
      421 tacaaaaagg aagtatcgaa gagggagttt ctaagaaatc attcaaaaat tctcacgtcg
      481 tgtcactgac agcgatggga gaagcgatta gtgagttaaa atcaggcaag gtagaagctg
      541 ttgatttaga aaaacccgta gctgaaggtt acttggccca gaatacagac cttgttcttg
      601 ctaagtttgc ccttaaaaca ggagaagggg atgctaaggc tgtagcttta cctaaagaca
      661 gtggaaaaat gttgaagact gttaacaaag tgataaagag acttgaaaaa gaagataaat
      721 ataaagttta tatttcagat gcggctaaat taactggcaa tcaagttgaa taatattaaa
      781 aagcctagtc ataggctttt ttggatgtta taatatggct tgactaagaa taatagcata
      841 aaggcagagg aatatatggt tcagggttca attaatattg aaaaatataa agatttggcg
      901 ctccaaaaac aaggcgagca tcgtaaattt ttagcaagtt taaaaaagaa agctcctaaa
      961 aacttagata aaatagtcca acagatacat caagaggttt ttcaagaaat tgattgtaca
     1021 gcatgtgcta attgttgtaa aactttgggt cctctattta cagaagctga tatttctagg
     1081 atttctaaac atttccggat gaagttatcg gcttttgaag atttattttt agaagtagat
     1141 gaagatggag ataaagtatt taaagcgatg ccttgtccgt ttttaggcga tgataactta
     1201 tgtagtatat acgatgtcag acctaaagct tgtcgtgaat ttccacatac tgatcgtaag
     1261 aaaatctatc aaattaataa cttaacgata aaaaatactc ttacttgtca agcggcatac
     1321 ctttttgtgg aaaaattgaa atcacgtata gggaaataat aacccagaga aagtatcata
     1381 aaaatattga caatctgctt ttatttgggt atcctagtaa gtgaacgtga aaatgttaca
     1441 atatcttgag gaggtgaaac atatgtcaaa aacagtagta cgtaaaaacg aatcacttga
     1501 cgatgcactt cgtcgtttca aacgttctgt gactaaagct ggtactcttc aagaatcacg
     1561 taaacgtgaa ttctacgaaa aaccttctgt aaaacgtaaa cgtaaatcag aagcagctcg
     1621 caaacgtaaa aaattctaat t
//
LOCUS       DBJYSH010000184         1618 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.184, whole genome shotgun sequence.
ACCESSION   DBJYSH010000184 DBJYSH010000000
VERSION     DBJYSH010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1618
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.184"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            43..1611
                     /locus_tag="ACUBV3_001970"
     CDS             43..1611
                     /locus_tag="ACUBV3_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HHU3848439.1"
                     /translation="MNLQKNDKTNSFIHYANGPSLEEINGTIEVPKDLSFFKTLLAYS
                     GPGALVAVGYMDPGNWSTSITGGQNFQYLLISIILMSSLIAMLLQYMSAKLGIVTQMD
                     LAQAIRARTSKQLGIVLWILTELAIMATDIAEVIGGAIALYLLFHIPLAIAVFITVFD
                     VLLLLLLTKIGFRKIEALVVALILVIFLVFAYQVALSHPIWTDIFKGLVPTSEAFSTS
                     HTVNGQTPLSGALGIIGATVMPHNLYLHSSVVQSRKLDHNNKKDIARAIRFSTFDSNI
                     QLTVAFFVNSLLLIMGVAVFKTGSVTDPSFFGLFKALSNSTIMSNSILAHIASSGILS
                     LLFAIALLASGQNSTITGTLTGQIIMEGFIHMKVPIWFRRIITRLISVIPVMICVLVT
                     SGRSTVEEHIAINNLMNNSQVFLAFALPFSMLPLLIFTNSKVEMDDDFKNTWIIKILG
                     WLSVIGLIYLNMKGLPNQIEGFWGDNPTHQQIILAHQIAHIIIISVILTLIWTIVELY
                     KGNKKYSTMQQSAT"
BASE COUNT          507 a          255 c          259 g          597 t
ORIGIN      
        1 attatttaac catactattt caatggaaag gtttatagtt caatgaatct acaaaaaaat
       61 gataaaacaa atagttttat ccattatgca aacggtcctt ccctagaaga aataaatggg
      121 acaattgaag ttcctaaaga tttgagcttt tttaaaactt tactagctta ttctggaccg
      181 ggtgcacttg ttgctgttgg ttatatggat cctggaaatt ggtccacttc cataactggc
      241 ggtcaaaatt ttcaatattt actaatttca attatcttaa tgtctagttt aattgctatg
      301 ctacttcaat acatgtcagc taagctaggt attgttactc agatggatct tgctcaagct
      361 atccgagcaa ggaccagtaa acagttggga attgtcttat ggattctcac tgagcttgct
      421 ataatggcaa cagatattgc tgaagttatt ggtggggcta ttgcacttta tttactgttt
      481 catattccac ttgcgattgc tgttttcatt acagtatttg atgtattact cttattattg
      541 ttgacaaaaa taggatttcg aaaaattgaa gctctagtag ttgctttaat attggttatt
      601 ttcttagttt ttgcatatca agtggctctt tctcatccta tatggacgga tatttttaaa
      661 ggtttagtac ctacatctga agctttctca acctctcata cagtaaatgg acaaacacca
      721 ctttcaggtg ccttaggcat tattggggca actgttatgc cacataatct ttatctacat
      781 tcttcagttg ttcaaagtcg aaaactagat cataataaca aaaaagatat cgctagagca
      841 attaggtttt caacttttga ttcaaatatt caattaacag tagccttttt tgttaattca
      901 ctattactca ttatgggcgt tgctgttttc aaaacaggaa gtgttactga tccatcattc
      961 tttggtctat ttaaagcact atctaattct actattatga gtaattctat tttagctcat
     1021 atagcaagtt caggtatttt atcactttta tttgcaatag ctttattagc atctggacag
     1081 aattcaacta ttactggaac attaacaggt caaattatta tggaaggatt tattcatatg
     1141 aaagttccaa tttggttccg tcgtatcatt actcgcctaa tttctgttat tcctgtaatg
     1201 atttgtgtct tagtaactag tggtagaagt actgttgaag aacatatagc tattaataac
     1261 ttaatgaata actctcaagt cttcttagca tttgcattac cattctcaat gttacctcta
     1321 ctaatattta ctaatagtaa agttgaaatg gatgatgatt ttaaaaatac ttggataatt
     1381 aagattcttg gttggctttc agttatagga ttaatttatc tcaatatgaa gggattgcca
     1441 aatcaaattg aaggtttttg gggagataac ccaactcatc aacaaattat tcttgcgcat
     1501 caaatagctc atatcatcat tattagtgta atactaactt taatatggac tattgttgaa
     1561 ttgtataaag gaaataaaaa atacagtacg atgcaacaaa gtgcaactta atttagta
//
LOCUS       DBJYSH010000185         1600 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.185, whole genome shotgun sequence.
ACCESSION   DBJYSH010000185 DBJYSH010000000
VERSION     DBJYSH010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1600
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.185"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            21..479
                     /locus_tag="ACUBV3_001971"
                     /pseudo
     CDS             21..479
                     /locus_tag="ACUBV3_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647095.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            543..863
                     /locus_tag="ACUBV3_001972"
     CDS             543..863
                     /locus_tag="ACUBV3_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3848440.1"
                     /translation="MSNWNLDNFDNLHSSLAESAYNSRPNSFPELFETDSVTEVKFSQ
                     PSEDNKGQITQGGTNLPNDGIVYLQPDKSLKSIDENVKVRVPDVNGGYHSEHCVTNSY
                     QKGY"
BASE COUNT          583 a          211 c          248 g          558 t
ORIGIN      
        1 ttctaagaaa agagagcatg atgaaaaaat atgtaaaatg gttaattccc atatctatta
       61 ttgggatgtt actagcaggc tgtcaaatga atagtgaatc taaaagtcag tcaaacgaag
      121 taaaaaatag caaacactca gcagtaaaga aagataaaaa aatgacaaaa aaagaacaat
      181 tagtttatct caatgagcat gaacaagaaa taattgattt tgtaaaatca cataatcaac
      241 aaattgagtc cgttcaattc gattggtcag gtataaaagt agaataaagt ggaaatggaa
      301 ctccacaagg gggtgattat aatctttcac tgagaggaaa gtttaatcat ctacaaaatt
      361 caaaattaat agttgatttt tatttagctc ataaaaatga tatcccaaat atcaaatcaa
      421 tgggaatgct aaataagccg tatatacata aaaatggtat ttggcacatt tatgaatagc
      481 ttgaatcatc ctatataaag taatagaatt tttaccacaa gcaaggtaga ggagaagaat
      541 aattgagtaa ttggaactta gataattttg ataacttaca tagctcttta gcagaaagtg
      601 catataatag tcgacctaat tcatttcctg aattatttga gactgactca gtaacagaag
      661 taaaattttc tcaaccctca gaagataaca aaggtcaaat cacacagggt ggcactaacc
      721 tccccaatga tggtattgtc tacctgcaac cagataagtc cttaaaatct attgatgaaa
      781 atgtaaaggt tcgcgttcct gatgtcaatg gcggttatca ctctgagcat tgtgttacta
      841 attcctatca gaaaggttac tgaaagatga tgaagcagta actctataat cactaaaagc
      901 tcatcattgc ttagtgttta agttaacttc taaaactcaa accttattaa atcaagattt
      961 gtttactttt aaaagtgaca ttttattttt ctaaaaatga gattattggg attcctcatt
     1021 ataagttact ttgtttatat gtacctttac ttaagggtta ttttcattat ttttaattta
     1081 atattttgga gtatgtatgc tccattatag tattttcgaa gacatcttta aaaggactgt
     1141 agaagttaaa atattttaac ctaatatcta acgtcatata ttggtacata gttttaagta
     1201 tccttttgat aaccttgtac atatgaaata ggctaaatat ttttagtgaa ctaaatgtaa
     1261 gtttattgct tgtattaggc gggataacaa tattgttgtt tttattcttt ctgagtaatg
     1321 gtttattaat tatgtaatta ttctggtatc aaatgatact tttaatattc cagtttgtca
     1381 ataaatcatc aatgtaattc ttttcatcaa aagcgatccc tttatctggt tgatttaata
     1441 tgttaacttg atgattattc ttcttgctta atatatggga taataattat cttaaattga
     1501 cacttggagc attcaatcac gagattgtaa taactaaaaa ttgtttaaaa tttaaggatg
     1561 tatttcttgt tgatcttact atctgatcta ataatggtat
//
LOCUS       DBJYSH010000186         1550 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.186, whole genome shotgun sequence.
ACCESSION   DBJYSH010000186 DBJYSH010000000
VERSION     DBJYSH010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1550
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.186"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            71..649
                     /locus_tag="ACUBV3_001973"
     CDS             71..649
                     /locus_tag="ACUBV3_001973"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HHU3848441.1"
                     /translation="MSNLDILYCKLAMCTTLLTERLRLQPVDLSNINDFLEFSSDSET
                     VFYMQRYKANTTEEAQMVLANVCMKSPLGIYAMIETESNKMIGIIELEIRDEFSAEFG
                     YILNKNYSGKGYMTEACSKLMSVGFEYLDLERIYARFDINNKKSGNVMERLGMKKEGE
                     LRHLAKNPKGEWKTRAYYSILKEEYFNKVNTK"
     gene            692..1372
                     /locus_tag="ACUBV3_001974"
     CDS             692..1372
                     /locus_tag="ACUBV3_001974"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HHU3848442.1"
                     /translation="MGDVVENFTEGKNPKIDTLNGKTVRIEKINPDHFEDLFQVYGEL
                     STEDSLTYISFSKFNSKNEFDVFFQTLLKSEDPYYLAIVDNNTGKALGTFSLMRIDTK
                     NRVVEMGWVVYSSKLKQTRIATEAQYLVMKYVFEELCYRRYEWKCDSLNAPSNNSAKR
                     LGFTFEGTFRQAVVYKGRNRDTNWYSILDKEWPEKKTRFEKWLDDSNFDVNGYQIRSL
                     SSIEQKTK"
BASE COUNT          573 a          179 c          294 g          504 t
ORIGIN      
        1 ttataatatt gtgtttttgg agattattga agtggtttat agataattaa ggaaggaata
       61 ttggttagaa atgagtaatt tggatatact atattgtaag ttagcaatgt gcactacatt
      121 actaacggaa agactacgac ttcagcctgt tgatttatct aatataaatg actttttaga
      181 attttcatct gattctgaaa ccgtatttta tatgcaacga tataaagcca ataccacaga
      241 agaagcacaa atggttttgg cgaatgtctg tatgaaatca ccattaggaa tctatgcgat
      301 gattgagaca gaatcaaata aaatgattgg tattatagaa ttagaaatta gagatgagtt
      361 ttcagcagaa ttcggttata tattaaataa aaattatagt ggtaaaggtt atatgacaga
      421 agcttgttca aaattaatgt cagttggttt tgaatatcta gacttggaaa gaatttatgc
      481 tcgtttcgac attaataata aaaagtctgg aaatgttatg gaacgtttag gtatgaaaaa
      541 agagggcgaa cttcgtcatc ttgcaaaaaa tccaaaaggt gagtggaaaa caagagcgta
      601 ttattctatt ttaaaagaag aatattttaa taaagttaat actaaatagt ctgagatgta
      661 gagaattaga tataatcttt ttaatcagcc agtgggtgat gtcgttgaaa attttacgga
      721 aggtaaaaat ccgaagattg atacacttaa tgggaaaaca gttagaatag aaaaaataaa
      781 tccagatcat tttgaagact tatttcaagt ttatggagaa ttgtctactg aagatagttt
      841 aacttatata tcttttagca aatttaatag taagaatgaa tttgatgtct ttttccaaac
      901 tctattaaaa tcagaagatc cttattattt agctattgtt gataataata ctggtaaagc
      961 attagggact ttttcattaa tgcgtattga tacaaaaaat agagttgttg aaatgggatg
     1021 ggtggtttat tcatcaaaac taaaacaaac aaggatagca acagaagctc aatatttggt
     1081 tatgaaatat gtttttgaag aactctgtta ccgccgttat gagtggaaat gtgatagttt
     1141 gaatgcacca tctaataatt ctgcaaaacg acttggtttt acttttgaag gaacttttag
     1201 acaagctgtc gtatataagg gtcgtaacag agatacaaat tggtattcta ttttagataa
     1261 agagtggcca gagaaaaaaa cacggtttga aaaatggcta gatgattcaa actttgatgt
     1321 aaatgggtat caaatcagat cactatcaag cattgagcag aagacaaaat agagtcattc
     1381 attaatcact aaaatttcaa aattacatgt ttttttagct aacatcttaa actttgttaa
     1441 ataaatgttt attaactttt aaaggtgcat ggtaggactc aaaatcgggt gtcctcacgg
     1501 acgtgccagc tcgactttgg tcgcgggcat caaagaaaaa agtcaagatt
//
LOCUS       DBJYSH010000187         1509 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.187, whole genome shotgun sequence.
ACCESSION   DBJYSH010000187 DBJYSH010000000
VERSION     DBJYSH010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.187"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            166..411
                     /locus_tag="ACUBV3_001975"
     CDS             166..411
                     /locus_tag="ACUBV3_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HHU3848443.1"
                     /translation="MGLIWSLIVGAIIGAIAGAITNKGGSMGWIANILAGLVGSFVGQ
                     SLLGTWGPKLADMALIPSIVGAIIVIIVTSFVLGKKS"
     gene            459..707
                     /locus_tag="ACUBV3_001976"
     CDS             459..707
                     /locus_tag="ACUBV3_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HHU3848444.1"
                     /translation="MSLIWSLIVGAIIGAVAGAVTNKGGSMGWIANILAGLVGSFVGQ
                     SLLGTWGPKLAGMALIPSIVGAIIVVIVTSFVLGKMNN"
     gene            722..1303
                     /gene="amaP"
                     /locus_tag="ACUBV3_001977"
     CDS             722..1303
                     /gene="amaP"
                     /locus_tag="ACUBV3_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HHU3848445.1"
                     /translation="MSKGLKSLYTLLGLISLTLLGFVAVISKQHIYLPSFNWLDWDFN
                     LPSPIDVGMYHYFFWGALVLFVIVLLAILVVLFYPRRYTEYKLADKTGKLMLKKSAIE
                     GFVKTEVLKTGLMKSPSVTAHLYKKKVKVDVKGLLTSRTNVPEQLEHIQSDVELGLKE
                     FFGLEKKMNTRVFVKQVEEENVGNAKTNKSRVE"
     gene            1313..1507
                     /locus_tag="ACUBV3_001978"
     CDS             1313..1507
                     /locus_tag="ACUBV3_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HHU3848446.1"
                     /translation="MSEFVRKYRYPLGGAVIGLVLAAMIVTIGFFKTILALVIIVLGA
                     YAGLYVQRTGMLDQFFNKRK"
BASE COUNT          467 a          196 c          324 g          522 t
ORIGIN      
        1 tttaccaagc tttttacttg gcagtcactt tataaagttg tataatatta gtatagttaa
       61 caacttaatt aattgccgag tggtcacttt agaaactctt gttatttagt taagaacaaa
      121 ataagtttgt acactgagat gtacttagga agaaaggtat ttatcatggg gttaatttgg
      181 tcattaatcg ttggtgctat tattggtgct attgcaggag caatcactaa caaaggtggt
      241 tcaatgggtt ggatagctaa catcttagct ggtttagttg gttcatttgt tggtcaaagt
      301 ttactaggaa catgggggcc taagttagca gatatggctt tgattccttc aatcgtaggt
      361 gcgattatcg ttatcattgt tacttcattt gtattaggca aaaaatcata actacataac
      421 tagtagaatg attagtcaag aaagagaagg tatttatcat gagtttaatt tggtcattaa
      481 tcgtaggtgc tattatcggt gctgttgcag gggctgttac taacaaaggt ggttcaatgg
      541 gttggatagc taacatctta gctggtttag ttggttcatt tgttggtcaa agtttactag
      601 gaacatgggg gcctaaatta gcaggtatgg ctttgattcc ttcaatcgta ggtgcgatta
      661 ttgttgtcat tgttacttca tttgtactag gtaaaatgaa taactagaaa aggagttacc
      721 tatgtcaaaa ggactaaaaa gtttatatac tttgctaggt ctgatctccc ttaccctact
      781 aggatttgta gctgttatca gtaaacaaca catttatcta ccatctttta attggttaga
      841 ttgggatttt aatttgccat caccgattga cgttggtatg tatcattatt tcttttgggg
      901 agcattagtg ctgtttgtaa tcgttttact ggctatattg gtagttctgt tttatccaag
      961 acgatacaca gagtataaac tggctgataa aactggtaaa ttgatgttaa aaaaatctgc
     1021 aatagaggga tttgttaaaa cagaagtatt aaaaactggt ttgatgaaat ctcctagtgt
     1081 gacagctcat ttatataaga aaaaagtgaa agttgatgtt aaaggtcttt taacttctcg
     1141 tacaaatgta ccggaacaac ttgaacatat tcaaagtgat gttgaacttg gcttaaaaga
     1201 attctttggg ttggaaaaga aaatgaacac tagagtattt gttaagcaag ttgaagaaga
     1261 aaatgttgga aatgctaaga cgaataaaag tcgtgtagaa taggagtcta atatgtcaga
     1321 attcgtgagg aaatacagat atccactagg aggtgcagtt attgggctcg ttttagctgc
     1381 aatgattgta accattggat tctttaaaac aattcttgct cttgttatca ttgtgctagg
     1441 tgcttatgct gggctttatg tgcaaagaac aggtatgctt gaccaattct ttaataagag
     1501 aaaataaaa
//
LOCUS       DBJYSH010000188         1486 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.188, whole genome shotgun sequence.
ACCESSION   DBJYSH010000188 DBJYSH010000000
VERSION     DBJYSH010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1486
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.188"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            182..1474
                     /locus_tag="ACUBV3_001979"
     CDS             182..1474
                     /locus_tag="ACUBV3_001979"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000205037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HHU3848447.1"
                     /translation="MTSVVVVGTQWGDEGKGKITDFLSADAEVIARYQGGDNAGHTIV
                     IDNKKFKLHLIPSGIFFKEKISVIGNGVVVNPKSLVKELAYLHGEGVTTDNLRISDRA
                     HVILPYHIKLDQLQEDAKGDNKIGTTIKGIGPAYMDKAARVGIRIADLLDREVFAERL
                     KINLAEKNRLFEKMYDSTPLEFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDEVGDRIREIGKEYGTTTGRPRRVGWFDSVVMRHSRRVSGITNLSLNSIDVL
                     SGLDTVKICVAYDLDGKRIDYYPASLEQLKRCKPIYEELPGWSEDITACRSLDDLPEN
                     ARNYVRRVGELVGVRISTFSVGPGREQTNILESVWSNI"
BASE COUNT          443 a          245 c          319 g          479 t
ORIGIN      
        1 tttttttaca cttctaataa ttattttttt ctttttttcc gataatattt gaggaatttg
       61 gcaaaaaaaa acattttttg ctataataag gtgtcagaca aactcgcagg tgaaattcct
      121 gccatgtttt tccagaaagg attggggagt cccaaaaagg ctcactcagt gttaaataaa
      181 tatgacttca gttgttgtag taggtaccca atggggtgac gaaggtaaag gtaaaattac
      241 agatttttta tctgctgacg cagaagtaat tgcacgttat caaggtggtg ataacgctgg
      301 gcatactatc gttattgata acaaaaaatt taaacttcat ttaattcctt caggaatttt
      361 cttcaaagaa aaaatttctg tcattggtaa tggtgttgtc gttaatccga aatcattagt
      421 taaagagtta gcttatcttc acggagaagg tgtaacaacg gataaccttc gtatttcaga
      481 ccgtgcgcat gttatcttgc cataccacat caagcttgac caactacaag aagatgctaa
      541 aggtgataat aaaattggaa caaccatcaa aggaattggg ccagcttaca tggacaaggc
      601 tgcgcgtgtc ggtatccgta ttgctgacct tcttgataga gaagtttttg ctgagcgttt
      661 gaaaattaat ttagctgaga aaaatcgttt atttgaaaaa atgtatgaca gtacacctct
      721 tgaatttgat gatattttcg aagaatatta tgaatatggt caacaaatta agcaatatgt
      781 gactgataca tccgttatct taaatgatgc tcttgatgcc ggtaaacgtg ttctttttga
      841 aggagcacaa ggagttatgc tcgacattga tcaaggaaca tacccatttg taacatcttc
      901 caatccagta gcaggtggtg tcacaattgg ttcgggagtt ggaccaagta aaattaataa
      961 agtagtaggt gtatgtaaag cttacactag ccgtgtcggt gatggaccat tcccaacaga
     1021 actttttgat gaggttggtg accgtattcg tgagattggt aaagagtatg gtacaacgac
     1081 tggtcgtcct cgtcgcgttg gatggtttga ttctgttgtt atgcgtcaca gccgtcgagt
     1141 atcaggtatt actaatctct ctctgaattc aattgatgtt ctttcagggc ttgatacggt
     1201 gaaaatttgt gtggcttatg accttgatgg gaaacgtatt gactattatc cagcaagcct
     1261 tgaacagcta aaacgttgta aaccaatcta tgaagaatta ccgggctggt ctgaagatat
     1321 tacagcttgc cgtagcttag atgatcttcc agaaaatgca cgtaattacg ttcgccgtgt
     1381 tggcgaattg gttggtgttc gtatttctac tttctcagta gggcctggtc gtgaacaaac
     1441 aaacattctt gagtctgttt ggtcaaatat ttaatattaa aagtct
//
LOCUS       DBJYSH010000189         1485 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.189, whole genome shotgun sequence.
ACCESSION   DBJYSH010000189 DBJYSH010000000
VERSION     DBJYSH010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1485
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.189"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            139..1422
                     /locus_tag="ACUBV3_001980"
     CDS             139..1422
                     /locus_tag="ACUBV3_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HHU3848448.1"
                     /translation="MSLYYTEKAHAIAGPSDRQYVENPNPHIIVNVTGTDQNGNSILP
                     HYIEVNVKMGQTLSKEEILDYIARNLNSSVGGESKNVQYSNIEFKESAYLKRQLDDGK
                     TEEIAIDNDGVTVPKDGPNKFWIDVPVTCTVTPIVTETHEVRWGTPVAISHRIYFVEE
                     SSGKVLDEYTNLHTADSELNGYRVGDYITDYALSKSAYEAFLNSRLDKEGYKLQHRIS
                     TNVRQNLQINKLIFNYDFNEENIYYQIGNIRPLLSRSSAEVESDIITERYYVSKNAKS
                     LARTESTISIKMVDAKTEQPLFNHTLTGYQLVTVSHVYNRLFEENLIPTTKSGERYFI
                     QNMKKTAEQEYTVYLSETPYSKENAPVISYDARPVDWDYHSGASGSLENQPNIYTEED
                     STEFLGNKPQAACYPNKQFACENTDSKYNYSYLEK"
BASE COUNT          532 a          267 c          240 g          446 t
ORIGIN      
        1 agagattaat tcctatcatt cactgcttta ttttaggata actaaacaat ttattgttta
       61 aaatgaaagg atttatgaat gtctaggaaa acatttaaac gtatactatc gattggagtt
      121 tgcacgctcg tactatcgat gagtctttat tacactgaaa aagcccacgc tattgctggt
      181 cctagtgacc gccaatacgt agaaaaccca aatcctcaca ttattgtaaa tgttacaggt
      241 actgatcaaa acggaaatag cattttaccg cattatatcg aagtcaatgt aaagatggga
      301 caaactttaa gtaaagaaga aattctagat tatattgctc gaaatttaaa ctctagtgtt
      361 ggaggagaaa gtaaaaacgt tcaatacagc aacatcgagt ttaaggaaag tgcttatctg
      421 aagcgtcaat tagatgatgg caagacagaa gaaatagcaa ttgataacga cggtgttact
      481 gtacctaaag acggtccaaa caaattttgg attgacgttc cagtaacttg tactgttact
      541 cctatcgtaa cagaaacaca tgaagttcga tgggggactc cagtcgctat atcacaccgt
      601 atttactttg ttgaagaatc ttctggaaaa gttttagatg aatacacaaa tctacacact
      661 gctgattcgg aacttaacgg ttatcgtgtt ggagattata tcacagacta tgcactttct
      721 aagtctgctt acgaagcttt tttaaactct cgtttagata aagaaggtta caaacttcaa
      781 catcgtatta gcacgaatgt acgacaaaac cttcaaatta ataaattgat tttcaattat
      841 gactttaatg aggaaaatat ttactaccaa atcgggaata tccgtccact attaagtcgc
      901 tcatcagctg aagtagaatc tgacatcatt acagaacgct actatgtttc taaaaatgct
      961 aaaagtttag cacgtacaga atcaaccatt tcgattaaaa tggttgatgc caaaactgaa
     1021 caaccgctat ttaaccacac attaactggt tatcaattgg taactgtctc ccatgtctat
     1081 aacagactct ttgaagaaaa tcttatccca actacaaaat caggagaaag atattttatt
     1141 caaaatatga aaaaaacagc tgaacaagaa tatactgttt atctttcaga aacaccttat
     1201 tctaaagaga acgctcccgt aatttcttat gatgcaagac ctgttgattg ggattatcac
     1261 tcaggcgctt caggatcact tgaaaatcag cctaacatct atactgaaga agattcaact
     1321 gaatttttgg gtaataaacc acaagcagct tgttatccaa acaaacaatt tgcttgcgaa
     1381 aatactgact ctaaatacaa ctatagctat ttagaaaaat agttataacc gatagggtta
     1441 atctctcatt atcttatact gattaaaatc attaaacttg tttcc
//
LOCUS       DBJYSH010000190         1474 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.190, whole genome shotgun sequence.
ACCESSION   DBJYSH010000190 DBJYSH010000000
VERSION     DBJYSH010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1474
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.190"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            complement(234..557)
                     /locus_tag="ACUBV3_001981"
     CDS             complement(234..557)
                     /locus_tag="ACUBV3_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HHU3848449.1"
                     /translation="MINSTVFLAILLGFVVSWVPRVAPFIFVKYKGLPNSLVKFLKFL
                     PLSILFALTFSSFFLVKLGQLPTLKWLDLLAGIPTLVVTIRYRNLFYTVLTGIVTMAL
                     LRLLF"
     gene            complement(547..1239)
                     /locus_tag="ACUBV3_001982"
     CDS             complement(547..1239)
                     /locus_tag="ACUBV3_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HHU3848450.1"
                     /translation="MEKGFKEGVKDALPTALGYISIGLAFGIVASASDLSAIEVGLMS
                     ALVYGGSAQFAMCALLLAKADLMTITMTVFLVNLRNMLMSLHATTIFKSAHLMNQLAI
                     GTLITDESYGVLLGEALHHKVVSPSWMHGNNVMSYLTWVISTIIGTLLGSTIPNPEMF
                     GLDFALVAMFIGLFVFQLFGMLSDGKRLVVYVLASVGLSYFLLATFLSGALSVLLATV
                     VGCSVGVVLDDK"
BASE COUNT          558 a          283 c          206 g          427 t
ORIGIN      
        1 ccactatgga cctttttata aattttaaaa cagcagcatg tagatatctt taaaaagcaa
       61 tcttacaatc aactcatatt atcaagatac agaaaaatct gttgatatta ttaacaatta
      121 gtcaaactat tacacatcgg ctatcaaaag ttcctgtcta gacaaaaaca caaaaaacgt
      181 atctagaaat agatgcgttt tttgtgtttt tattaatctt ccattcctac acgttaaaat
      241 agtaatctca ataaagccat agtaacgata ccagtaagaa cagtgtaaaa taaatttcga
      301 tatcgaatag tgacaactag agttggtatg ccagccaata aatctaacca ttttagtgtt
      361 ggcaattgtc ctaatttaac taaaaaaaag ctagagaatg ttagggcaaa taaaatagaa
      421 agaggaagaa attttaaaaa tttcacaaga ctatttggta gccctttgta ttttacaaat
      481 atgaatggag cgactcttgg cacccaagaa acaacaaaac ctaaaagaat agcaagaaaa
      541 acggtactat ttatcatcta aaacaactcc cacactacat ccaacaacag tcgcaagcaa
      601 tactgataag gctcctgaca aaaaagtagc taataagaag taacttaaac ctacagaagc
      661 aagaacatat acaacaagtc gctttccatc agataacatc ccaaacaact gaaatacaaa
      721 gagacctata aacatggcga ctaatgcaaa gtctaaacca aacatttctg gattaggaat
      781 agtactcccc agtaaagttc ctattatcgt tgagattacc catgtcaaat aactcatcac
      841 attattacca tgcatccaac taggcgatac aactttgtga tgtaatgctt ctccaagtaa
      901 aacgccgtag ctctcatctg ttattagagt tcctatggct aattgattca ttaggtgagc
      961 agacttgaag atagtagtcg cgtgtaaact cattaacata tttctcaaat taactaaaaa
     1021 tactgtcatc gtaatagtca tcaagtctgc ttttgctaat aatagcgcac acatagcaaa
     1081 ctgagcagat cccccatata ccaaggcact cataagacca acttctattg cagataaatc
     1141 tgatgctgaa gcaactatcc caaacgccaa gcctattgag atatacccta aagctgtggg
     1201 caaggcatct ttcactccct ctttaaatcc tttttccatc atatccccct aaattttcag
     1261 tatattcagt tattatagca caacggtgat tactaagaaa gaagaggaat gattatttat
     1321 ctttgccagc aattagacta ataaatatcc tgtaattatt ctataaacct ttgatattgt
     1381 agccatatag ttggacaata ttattatttt catagaatga tttttaaaaa atgctttagg
     1441 tacggtctta aatttaccac aaaataattt gtaa
//
LOCUS       DBJYSH010000191         1394 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.191, whole genome shotgun sequence.
ACCESSION   DBJYSH010000191 DBJYSH010000000
VERSION     DBJYSH010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1394
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.191"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            complement(14..367)
                     /locus_tag="ACUBV3_001983"
     CDS             complement(14..367)
                     /locus_tag="ACUBV3_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HHU3848451.1"
                     /translation="MIYKIIASLFLVLIPIFSQVLVKIFKLKKFNIMFPDVAFPIFVF
                     LIPLISSSLLKQNLLPYYLILISLLAIGITIKLLRTKTLFSYKRFLKLFWRSGFILTF
                     LFYLGLLVIIFIKVQ"
     gene            complement(428..1333)
                     /locus_tag="ACUBV3_001984"
     CDS             complement(428..1333)
                     /locus_tag="ACUBV3_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HHU3848452.1"
                     /translation="MKNQQKLMYLESIELYSNITKAAAHLFISQPYLSKVIKQLENEL
                     EIKLIQSQGHQTFLTYAGQRYLFYLKEIDMIERQMAKELYLIRSDKKGEITLGINSGL
                     ASSILANVLPKFNLEHPEISVKLLENNQNISEQLVASGDIDLAVGMAPILYKDGIAST
                     TIYRDELFLMIPTTSQLYNAEKRGQIIPFEYPISVLDNEPLILTPLEYGIGKTIAQFY
                     ELHHMSLNQMITTSTVPTAASLSLSGMGATFVPQTLIHRYLDKECNVYHFHKNKLFSE
                     YIMIYKKDVELSGIALLLYKAFLTK"
BASE COUNT          478 a          190 c          257 g          469 t
ORIGIN      
        1 tatccaactc tttttattgt actttgataa agatgataac caataatcct aggtaaaaaa
       61 ggaaagttag aataaagcca gatctccaaa ataattttaa aaaacgttta taagaaaaaa
      121 gagtctttgt tcgtagaagc tttattgtaa ttccgattgc cagtaatgaa atgagaatca
      181 gatagtaagg taaaagattt tgttttaaca gactagatga tattaatgga attaaaaaaa
      241 caaaaatagg aaaagcaaca tctggaaaca taatgttaaa ttttttaagt ttaaatattt
      301 tgacaagaac ctgtgaaaat atagggatta aaacaagaaa taaagaagca ataattttgt
      361 atatcatagg tctattttat accgattttg gtaagaagta aactgatttt caatagaatt
      421 agcgaaacta cttggttaaa aaagctttat aaagtaggag agcaattcca gataattcaa
      481 catctttttt ataaatcata atgtactctg aaaataactt attcttatga aaatggtaga
      541 cattgcactc tttatccaaa tagcgatgaa tgagagtttg tggaacaaat gttgctccca
      601 tccctgacaa agataggcta gctgctgtag gaacggtact agttgtaatc atctgattta
      661 atgacatgtg atgaagctca tagaattgtg caattgtttt gccaattccg tactctaatg
      721 gtgttaggat aagaggttca ttgtctagaa ctgaaatagg atattcaaaa ggaataatct
      781 gtccacgttt ttcagcgttg tagagttggc tggtggtagg tatcattaaa aacaattcgt
      841 cgcggtaaat agtggttgat gcaattccat ctttataaag gataggagcc atcccaacag
      901 ctaggtcaat atcaccactc gctaccaatt gttcagaaat attttgattg ttttcgagta
      961 atttgacaga aatttctggg tgctccaagt taaatttggg aagtacattt gccagaatag
     1021 aacttgctaa gccagagtta atcccaagtg tgatttctcc ttttttatca gaacggatga
     1081 gatatagttc tttggccatt tgacgctcaa tcatatcaat ttcttttagg tagaatagat
     1141 atctctgtcc agcataagtt aaaaaggttt ggtgtccttg gctctggatg agttttattt
     1201 ctaactcatt ttctaattgc ttgatcactt tgcttaggta aggttgagaa ataaaaaggt
     1261 gtgcagcggc tttagtgatg tttgagtata gctcgatact ctctagatac attaattttt
     1321 gttgattttt caagacttta taaccagttt gatatcctta gattaataat aagttatgat
     1381 acgttatggc tata
//
LOCUS       DBJYSH010000192         1389 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.192, whole genome shotgun sequence.
ACCESSION   DBJYSH010000192 DBJYSH010000000
VERSION     DBJYSH010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1389
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.192"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            144..569
                     /gene="rplK"
                     /locus_tag="ACUBV3_001985"
     CDS             144..569
                     /gene="rplK"
                     /locus_tag="ACUBV3_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004226069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HHU3848453.1"
                     /translation="MAKKVEKLVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGMIIPVVISVYEDKSFDFITKTPPAAVLLKKAAGVEKGSGEPNKTKVATITR
                     AQVQEIAETKMPDLNAANLESAMRMIEGTARSMGFTVTD"
     gene            673..1362
                     /gene="rplA"
                     /locus_tag="ACUBV3_001986"
     CDS             673..1362
                     /gene="rplA"
                     /locus_tag="ACUBV3_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HHU3848454.1"
                     /translation="MAKKSKNLCAALEKIDSTKAYSVEEAVALAKETNFAKFDATVEV
                     SYNLNIDVKKADQQIRGAMVLPAGTGKTSRVLVFARGAKAEEAKAAGADFVGEDDLVA
                     KIQGGWLDFDVVIATPDMMALVGRLGRVLGPRNLMPNPKTGTVTMDVAKAVEESKGGK
                     ITYRADKAGNVQALIGKVSFDDAKLVDNFKAFNDVIVKAKPATAKGTYITNLSITTTQ
                     GVGIKVDPNSL"
BASE COUNT          473 a          250 c          269 g          397 t
ORIGIN      
        1 tttaaaatat aatgaaatta atggcttaaa actattgctt ttatttatga ttatagtata
       61 atggtaaggt atgttataaa catgcctgtg ggaggtaaaa atttctaatt accgccaaaa
      121 ccacaacagg aggattttta aaaatggcta aaaaagttga aaaacttgta aaacttcaaa
      181 ttcctgctgg taaagcaact ccagctccac cagttggacc agcacttggt caagcaggaa
      241 tcaacatcat gggattcact aaagagttta acgctcgtac agctgatcaa gctggtatga
      301 ttattccagt tgttatctca gtatatgaag acaaatcatt tgatttcatc actaaaacac
      361 caccagctgc tgttcttttg aaaaaagctg caggtgttga aaaaggatca ggtgaaccta
      421 acaaaactaa agttgcaaca attactcgtg cgcaagtaca agaaattgct gaaactaaaa
      481 tgccagattt aaacgctgca aaccttgagt ctgcaatgcg tatgatcgaa ggtactgctc
      541 gttctatggg attcactgtt actgactaat cagaaacacc cacataaccc gcaagacttc
      601 atcgtagttt gatgagtgac gtgggagatt ttaaatcaaa tcgattgacc acattacaag
      661 gagaatttta aaatggctaa aaaaagtaaa aacttatgtg ctgctcttga gaaaattgac
      721 agcactaaag catacagcgt agaagaagct gtagctcttg caaaagaaac taactttgct
      781 aaatttgatg caactgttga agtttcttat aaccttaaca ttgacgttaa aaaagctgac
      841 caacaaatcc gtggtgctat ggttttacca gcaggtactg gtaaaacttc acgtgttctt
      901 gtttttgctc gtggtgctaa agctgaagaa gctaaagcag caggtgcaga ctttgtcggt
      961 gaagatgact tagttgctaa aatccaaggt ggatggcttg actttgatgt tgttatcgca
     1021 acaccagata tgatggcact tgttggacgt cttggacgtg tccttggacc tcgtaacttg
     1081 atgccaaacc ctaaaactgg tacagtaact atggacgttg ctaaagcagt tgaagagtct
     1141 aaaggtggta aaattactta ccgtgctgat aaagcaggta acgtccaagc ccttattggt
     1201 aaagtttcat ttgatgatgc taaattagtt gataacttca aagcatttaa tgatgttatt
     1261 gttaaagcta aaccagctac tgcaaaagga acttacatca caaacctttc aattacaaca
     1321 acacaaggtg ttggtatcaa agttgatcct aactcacttt aatcaataaa aaactaggaa
     1381 ttccttcct
//
LOCUS       DBJYSH010000193         1381 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.193, whole genome shotgun sequence.
ACCESSION   DBJYSH010000193 DBJYSH010000000
VERSION     DBJYSH010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1381
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.193"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            complement(147..461)
                     /locus_tag="ACUBV3_001987"
     CDS             complement(147..461)
                     /locus_tag="ACUBV3_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HHU3848455.1"
                     /translation="MFSKKLKELRINNKLSQKQIAEQMNISQQAYAKWESGKNSPSLA
                     TLEKVANFFNLSIEELLSDGTVSLDNLLKAETITYQGKTLSEEDAFKVKEVLNIVIST
                     IK"
BASE COUNT          493 a          185 c          241 g          462 t
ORIGIN      
        1 taaaagctaa aggttatgta attaaaatac aagcgatagc ttatcaaaaa ctcttgcaaa
       61 aaagtgttaa agtatttatc aataatccta aaaagcactt atttgagtac agagttaaaa
      121 atttagatca ttgataattc aatagattat ttgattgtac taatgacaat gttcaaaact
      181 tcttttactt taaaggcatc ttcttctgat agtgtttttc cttgataagt aatagtttca
      241 gcttttaata agttgtcaag actaacagta ccatcagata gtaattcttc aatagataaa
      301 ttgaagaaat ttgcaacttt ctcaagtgtt gcgagagatg gagaattttt tccactttcc
      361 cattttgcat aagcttgttg agaaatattc atttgttcag ctatttgttt ttgagaaagc
      421 ttgttgttaa ttcttaattc ttttagtttt ttggaaaaca taaaatttct tgaatttacc
      481 tattgacaaa acaactataa ggttgtaaaa tgattataac aacgatatgg ttgttagtca
      541 attctttcta tttgatgttg agcaattaat aattagcaaa agacaacatc gtatagaaat
      601 taagagtgac taaataggtt gcccattgat aattaaataa aaagtgcgtc agacaacaca
      661 gctaagctaa gttgaataac acacgtaata aattctagca agtttattat aacgtgaaat
      721 tcttcaaaag taaagcaggt gtagtatact acacaagttg tctggcttgc ttttgaggtc
      781 tgaactttgc ttgtttaagc aaaaaaagct agatagaaga ctatctagca aatggttact
      841 ataaaaattg atgcaaaaca attgcagtta tgattatagt aattactgtg tgttcaacta
      901 agcaatgaaa gtataatcca agtaattgga agaatccttt catgatagca aacatcactt
      961 cggtgataat ttctatcata ccctacctcc ttattagact aactctggag agtcaattct
     1021 gttcaaagtt tgctttcagg tagatctaaa aggagttggg catgatgtag aatcaccaaa
     1081 tatattttat cattctagtg atgactaaac aagtaaagtt cagacctcaa aagcacacta
     1141 aggtttatgt tagttagcct gtgttgagca aaaaactaaa actgctacct cgcaaggggt
     1201 gttgacaatg attactaaaa acgtagagag gcggataagg tatggtattg tgggcggtgt
     1261 gttagatact atctagctaa tgaataaggt ggacttattc gaacgagtac ctatgtgtga
     1321 aacattgaga aagtagtatg cgaagataag agcatatttt ttaaattttt ataaggtaag
     1381 t
//
LOCUS       DBJYSH010000194         1360 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.194, whole genome shotgun sequence.
ACCESSION   DBJYSH010000194 DBJYSH010000000
VERSION     DBJYSH010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1360
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.194"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            57..1133
                     /locus_tag="ACUBV3_001988"
     CDS             57..1133
                     /locus_tag="ACUBV3_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HHU3848456.1"
                     /translation="MLGENIYLQRTQIGMTQENLSDYLHLTKTTISKWENNQAKPDID
                     YLILMANLFDISLDDLVGYQKTLSDDQRNQLIKDLKIKANVLSERDFFQEVKELSKQF
                     PNDFKTLLIMINMVLSNLTNLNDSEMKEWSLSTLNKIISKTVVESDLQTAIMFKTVVL
                     FHNLEYDEVINLYQNSPYKLGEELLLANSFFAKGDVYRAKQVLQVEIYQQILLVCEYL
                     LSLSTFEVEENKGDIFERLEHMIEAFQLNSLHPNTVIKCYYSLACHYSKSDDDKALGY
                     LKQCFKSLKQLLQRFELHGDTFFYTIDDWLETIPTGIAPPTSQLQVIERVEDLFQNSQ
                     FKELSGHKEFQQMIQKLNDLKKDY"
     gene            1135..1341
                     /locus_tag="ACUBV3_001989"
     CDS             1135..1341
                     /locus_tag="ACUBV3_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001090962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLDc N-terminal domain-containing protein"
                     /protein_id="HHU3848457.1"
                     /translation="MNTEIPNQLLPFLVPLIMLQGALIIISLVKLSKLKMTKHLSKPV
                     WFLVIIFLNIIGPIAFLILEGNNE"
BASE COUNT          489 a          199 c          231 g          441 t
ORIGIN      
        1 ggaactttta tttttgattt agaaactcta taatggacgt gaaagtagat tgaccgatgc
       61 ttggagagaa tatctattta caacgtactc agattggtat gacgcaagag aatttgagtg
      121 attatctgca tttaacaaaa actacaattt caaagtggga aaataatcaa gctaaaccgg
      181 atattgacta tcttattttg atggctaatc tttttgatat tagcttggat gacttagtcg
      241 gttatcaaaa aacattgtca gatgatcaaa gaaatcagct cattaaggat ctaaaaataa
      301 aagcaaatgt attgtctgag agagatttct ttcaggaagt taaggaatta tcaaaacagt
      361 tccctaacga ttttaagact ctgttgataa tgattaatat ggtcttgagc aacctaacca
      421 accttaatga ttcagaaatg aaagagtggt cactaagtac actaaataaa attattagta
      481 aaacagtagt tgagtctgac ctccagacgg cgataatgtt caaaacggtc gttctttttc
      541 acaaccttga atatgatgaa gtgataaacc tttaccagaa tagtccctat aaattaggtg
      601 aagaattact tttagctaat agcttttttg caaaaggcga tgtttaccga gctaagcaag
      661 tattacaggt tgaaatttat cagcagattc tacttgtatg cgagtatcta ttgagtttat
      721 caacttttga agttgaagaa aacaaaggcg atatttttga aaggcttgaa catatgatag
      781 aagcgtttca attaaactct ttgcatccca acacagtcat caagtgttat tatagtttag
      841 cttgtcatta tagtaaaagt gatgatgata aggcattagg atacttaaaa caatgtttta
      901 aatctttaaa acaattgctc caaagatttg aattacacgg tgatactttc ttttatacca
      961 tcgatgattg gttagagaca atacctactg gtattgcacc accgacgagt caattacagg
     1021 taatagaacg agttgaggac ttatttcaaa attcacaatt taaagaacta tcagggcata
     1081 aagaatttca acaaatgatt caaaaactta acgatttaaa aaaggattac taatatgaat
     1141 acagaaatac caaatcaatt attaccattc ctagttccgt tgattatgtt gcaaggagct
     1201 ttaattatca tttctctagt caaactaagt aaacttaaga tgactaaaca cctttcaaag
     1261 ccagtttggt ttttggtcat tatatttctt aatataattg ggccaattgc atttcttatt
     1321 ttggaaggga ataatgaatg aaagctatct catttcaaaa
//
LOCUS       DBJYSH010000195         1359 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.195, whole genome shotgun sequence.
ACCESSION   DBJYSH010000195 DBJYSH010000000
VERSION     DBJYSH010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1359
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.195"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            complement(43..1278)
                     /gene="pbp3"
                     /locus_tag="ACUBV3_001990"
     CDS             complement(43..1278)
                     /gene="pbp3"
                     /locus_tag="ACUBV3_001990"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase PBP3"
                     /protein_id="HHU3848458.1"
                     /translation="MKKIITSILLLSCIFFMPTISAESFNASAKYALAVDLDSGKILY
                     EKDANRPAAIASLTKIMTVYMVYKEIDNGNLKWNTKVNISDYPYQLTRESDASNVPLE
                     KRRYTVKQLVDAAMISSANSAAIALAEHISGTESKFVDKMTAQLEKWGIHDSHLVNAS
                     GLNNSMLGNHIYPKSSQNDENKMSARDIAIVAYHLVNEYPSILKITSKSVAKFDKDIM
                     HSYNYMLPDMPVFRPGITGLKTGTTELAGQSFIATSTESGMRLLTVIMHADKADKDKY
                     ARFTATNSLLNYITNTYEPNLVLAKGATYKGKEASVRDGKEQSVIAVAKNDLKVAQKK
                     NITKQNQLKINFKKELTAPITKKENLGKAYYVDLNKVGKGYLIKEPSVHLVAKDSIER
                     SFFLKVWWNHFVRYVNEKL"
BASE COUNT          408 a          244 c          234 g          473 t
ORIGIN      
        1 aactgctgtg cagtaactcc ttgtgtcaaa gggagattac tattaaagtt tttcgttaac
       61 gtagcgcaca aaatgattcc accacacttt gaggaagaaa ctgcgctcaa tactatcttt
      121 tgccactaaa tgaacgctag gttcctttat gagatagcct tttccaacct tattaaggtc
      181 aacgtaataa gctttcccta ggttctcttt ttttgtaata ggagcagtaa gctctttttt
      241 aaagttaatt tttaactgat tttgtttagt gatatttttc ttctgtgcta ctttcaaatc
      301 gtttttagca acagcgataa ccgattgttc ttttccgtct ctcacacttg cttctttacc
      361 tttatatgta gctcctttag ctaatacaag gttaggttcg taggtgtttg tgatatagtt
      421 caagagagag ttagttgctg taaagcgagc atatttgtct ttatcggcct tatcagcatg
      481 cataataaca gtgagtagtc tcattccact ttcagtagat gtagcaataa aagattggcc
      541 agctaattcc gttgtcccag ttttcaaacc tgtaatacct ggtctaaaga caggcatatc
      601 tggtagcatg tagttataag aatgcataat atctttatca aatttagcaa cggacttact
      661 agtaatctta agaatggaag gatattcgtt gaccaaatgg taggcaacaa tagcaatatc
      721 acgtgcactc attttatttt cgtcgttttg tgacgatttt ggataaatgt gattgcctaa
      781 catactatta tttaagccag aagcattgac taggtggcta tcatgaattc cccacttttc
      841 caattgagca gtcattttat caacaaattt actttcagtt cctgaaatat gttcagccaa
      901 agcaatggct gcactgttag cactagaaat catggcagcg tccacgagct gtttaacagt
      961 atagcgcctt ttttctaaag gaacattact agcatcagat tcacgtgtta gttgataagg
     1021 gtagtcagat atatttactt tggtattcca cttgaggtta ccgttatcaa tttctttata
     1081 gaccatataa acggtcatta ttttagtcaa ggaagcaata gcagcgggtc tgttagcatc
     1141 tttttcatac aagatttttc ctgaatctaa atcaactgcc aaggcatatt tagcggaagc
     1201 attaaaagat tcagcagaga tggttggcat aaaaaaaatg caactaagta atagaataga
     1261 agtaataatt tttttcaaaa taacaccttt agaaattttt taaactttgt ccttcctatt
     1321 ataccatatc actataccat actaggaatt attattttc
//
LOCUS       DBJYSH010000196         1335 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.196, whole genome shotgun sequence.
ACCESSION   DBJYSH010000196 DBJYSH010000000
VERSION     DBJYSH010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1335
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.196"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            267..>1335
                     /gene="xerS"
                     /locus_tag="ACUBV3_001991"
     CDS             267..>1335
                     /gene="xerS"
                     /locus_tag="ACUBV3_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012658412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HHU3848459.1"
                     /translation="MKRELLLEKIDELKEIMPWYVLEYYQSKLSVPYSFTTLYEYLKE
                     YRRFLEWLLDSGVANCHHIAEIELSVLENLTKKDMEAFILYLRERPLLNANTRQNGVS
                     QTTINRTLSALSSLFKYLTEEVENADGEPYFYRNVMKKVSTKKKKETLASRAENIKQK
                     LFLGNETIEFLEYIDCEYQNKLSKRALAFFNKNKERDLAIIALLLASGVRLSEAVNLD
                     LKDINLNVMVIDVTRKGGKRDSVNVASFAKPYLANYLDIRKNRYKAENQDIALFLSEY
                     RGVPNRIDSSSVEKMVAKYSQDFKVRVTPHKLRHTLATRLYDATKSQVLVSHQLGHAS
                     TQVTDLYTHIVNDEQKNALDKL"
BASE COUNT          470 a          216 c          219 g          430 t
ORIGIN      
        1 aattgagtac actttattac cttttcagat accgataaaa tcaatgaaat tatgaatcag
       61 ttttctcaaa atactctgtc tttactataa caaacatgtt aaacgataat aagtaatact
      121 gatcatcata aagaatggat atttgatgta cttaccgaat gaattcctgt ttttaacttt
      181 taaactttcc gaaaaactat aattttcttg aaagttatga tagagtatga tatacttcta
      241 tttatagata taatggagat tttattatga aacgtgaatt actactcgaa aaaatcgatg
      301 aactaaaaga aattatgcct tggtatgttc tagaatatta tcaatcaaag ctttctgttc
      361 cttatagttt cacaacactc tacgaatatt taaaagaata tagacgtttt cttgaatggt
      421 tattggattc tggtgttgct aattgtcatc acattgcaga aattgagctt tcagttttag
      481 aaaaccttac taaaaaagat atggaagctt ttatactata tttacgagaa cgccctttat
      541 taaatgctaa cacaaggcaa aatggggtgt ctcaaaccac cattaacaga actctatctg
      601 ccttatctag tcttttcaaa taccttactg aagaggttga gaatgctgac ggggagcctt
      661 acttttatcg caatgtcatg aaaaaagtct caaccaaaaa gaaaaaggaa actttagctt
      721 ctcgggcaga aaatattaaa caaaagttat ttttgggcaa tgaaacgata gaattcttag
      781 agtatataga ttgtgaatat caaaacaaac tttccaaacg tgctttagct tttttcaaca
      841 aaaataaaga gagagattta gctattattg ctttgctact agcttcagga gtgcgtctat
      901 ctgaggcagt aaacttagat ttaaaggata ttaaccttaa tgtaatggtt attgatgtca
      961 cacgaaaagg ggggaaaagg gactctgtca atgtcgctag ctttgcgaag ccctaccttg
     1021 ctaattattt agacatccgt aaaaatagat acaaagcaga aaatcaggac attgctctat
     1081 ttttatcaga ataccgtggg gtgccaaatc gtattgattc ttcaagtgta gagaaaatgg
     1141 ttgctaagta ttcccaggat tttaaggttc gtgtaacacc tcataaacta aggcatactc
     1201 ttgctactag gctatatgat gctacaaaat ctcaagtatt agttagccac caactaggtc
     1261 atgcttccac tcaagttact gacctatata cccatattgt taacgatgaa caaaagaacg
     1321 ctttagataa actat
//
LOCUS       DBJYSH010000197         1298 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.197, whole genome shotgun sequence.
ACCESSION   DBJYSH010000197 DBJYSH010000000
VERSION     DBJYSH010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1298
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.197"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            99..>1298
                     /locus_tag="ACUBV3_001992"
     rRNA            99..>1298
                     /locus_tag="ACUBV3_001992"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          354 a          262 c          397 g          285 t
ORIGIN      
        1 gacgaacgaa tgtgcagggt ggatttacat tgtaaatccg tcaatgacaa aaaaacaata
       61 aatctgtcag cgagacagaa aagagcaaag ctcaaacttt taatgagagt ttgatcctgg
      121 ctcaggacga acgctggcgg cgtgcctaat acatgcaagt agaacgctga ggtttggtgt
      181 ttacactaga ctgatgagtt gcgaacgggt gagtaacgcg taggtaacct gcctcatagc
      241 gggggataac tattggaaac gatagctaat accgcataag agtaattaac acatgttggt
      301 tatttaaaag gagcaattgc ttcactgtga gatggacctg cgttgtatta gctagttggt
      361 gaggtaaagg ctcaccaagg cgacgataca tagccgacct gagagggtga tcggccacac
      421 tgggactgag acacggccca gactcctacg ggaggcagca gtagggaatc ttcggcaatg
      481 gacggaagtc tgaccgagca acgccgcgtg agtgaagaag gttttcggat cgtaaagctc
      541 tgttgttaga gaagaacgtt ggtaggagtg gaaaatctac caagtgacgg taactaacca
      601 gaaagggacg gctaactacg tgccagcagc cgcggtaata cgtaggtccc gagcgttgtc
      661 cggatttatt gggcgtaaag cgagcgcagg cggttcttta agtctgaagt taaaggcagt
      721 ggcttaacca ttgtacgctt tggaaactgg aggacttgag tgcagaaggg gagagtggaa
      781 ttccatgtgt agcggtgaaa tgcgtagata tatggaggaa caccggtggc gaaagcggct
      841 ctctggtctg taactgacgc tgaggctcga aagcgtgggg agcaaacagg attagatacc
      901 ctggtagtcc acgccgtaaa cgatgagtgc taggtgttag gccctttccg gggcttagtg
      961 ccgcagctaa cgcattaagc actccgcctg gggagtacga ccgcaaggtt gaaactcaaa
     1021 ggaattgacg ggggcccgca caagcggtgg agcatgtggt ttaattcgaa gcaacgcgaa
     1081 gaaccttacc aggtcttgac atccttctga ccggcctaga gataggcttt ctcttcggag
     1141 cagaagtgac aggtggtgca tggttgtcgt cagctcgtgt cgtgagatgt tgggttaagt
     1201 cccgcaacga gcgcaacccc tattgttagt tgccatcatt aagttgggca ctctagcgag
     1261 actgccggta ataaaccgga ggaaggtggg gatgacgt
//
LOCUS       DBJYSH010000198         1254 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.198, whole genome shotgun sequence.
ACCESSION   DBJYSH010000198 DBJYSH010000000
VERSION     DBJYSH010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1254
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.198"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            215..1237
                     /locus_tag="ACUBV3_001993"
     CDS             215..1237
                     /locus_tag="ACUBV3_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HHU3848460.1"
                     /translation="MNIIIGTSLLILVLAIFTLFNYKAPYGTKAMGALASAACASFLV
                     EAFQDSFFGKVLGFQFLSEVGGANGSLSGVAAAILVAIAIGVTPGYAVLIGLSVSGTG
                     IIPGFLAGYLVGFLVKWMERNIPGGLDLISIIIIGAPLTRLVAKLLTPLINSTLLTIG
                     DILTSGAHSNPILMGIILGGTIVVVATAPLSSMALTAMLGLTGMPMAIGALSVFGSSF
                     MNGVLFHKLKLGSRKDNIAFAVEPLTQADVTSANPIPIYVTNFVGGAACGILIALMKL
                     VNDTPGTATPIAGFAVMFAYNPMIKVLITALGCIILSLLAGYFGGIVFKDYKLVTKEE
                     LQARDN"
BASE COUNT          346 a          208 c          255 g          445 t
ORIGIN      
        1 ttatgctttt aggtaaaatg tgattatatt agcaactaag gagtaatcca tgagataagc
       61 ctaataatta tgtcggactt ctttgttgca ccttatccag ttcgtagtac aattaagttt
      121 cagtattacc taaaggcgcc tttattgaaa ttgaagcagt agtacacaga cactgagaat
      181 aaaaaactta actattttta gcgggagtat atttatgaat atcattattg ggaccagtct
      241 tttaatactt gtattagcga ttttcacttt atttaattat aaagcaccct acggaacaaa
      301 agcaatggga gcattggcat ctgcggcttg tgcatccttc cttgtagaag cttttcaaga
      361 ttctttcttt ggtaaagttt tgggatttca atttttaagt gaagtgggtg gtgctaatgg
      421 ttcactttct ggtgtagctg cagctatatt ggttgctatt gctattggtg taaccccagg
      481 atacgcagtt ttaattggtt tatctgtttc tggaacaggt attattcctg gtttccttgc
      541 aggatattta gtgggatttc ttgttaagtg gatggaaaga aatattcctg gtggtttaga
      601 tcttatttca attattatta ttggtgcacc attaacacgt cttgtggcca aactgttgac
      661 gccgcttatt aatagcactt tgctcaccat tggagatatc ttaacatcag gtgctcatag
      721 caaccctatt ttaatgggga ttatacttgg cgggacaatt gtagtagtgg cgacagcacc
      781 actttcttct atggcattga cagctatgct aggattaacc ggaatgccta tggctatagg
      841 agccttgtct gtctttggtt cgtcatttat gaatggtgta cttttccata aattaaaact
      901 tggaagtcgt aaagataata tagcttttgc tgttgagcct ctaactcaag ctgacgtgac
      961 ttcagctaac cctattccaa tctatgtcac taattttgtt ggtggtgcag cttgtggtat
     1021 tttaattgcc ttgatgaaat tagttaatga tactcctgga acagcgacac caattgcagg
     1081 atttgctgtc atgtttgcct ataacccaat gataaaagta ctaataaccg ctctaggttg
     1141 tattatccta tctttactag caggctattt tggaggcatt gtttttaaag attataaatt
     1201 agttacaaaa gaagagctac aggctagaga taactagtta cttcatgaaa agct
//
LOCUS       DBJYSH010000199         1231 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.199, whole genome shotgun sequence.
ACCESSION   DBJYSH010000199 DBJYSH010000000
VERSION     DBJYSH010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1231
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.199"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            complement(37..654)
                     /locus_tag="ACUBV3_001994"
     CDS             complement(37..654)
                     /locus_tag="ACUBV3_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017772020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3848461.1"
                     /translation="MRKFFQDVIVEVAYYLEIALSVILATAIIFFSLALFHDLFSMLS
                     VDQNVDKLFQTFLARAMTIAVGIELIKMFSKPSPDTLIEVLLFALARQLVIEHGSVSD
                     YLMGIASVAILFAVRKYLFTHFDGSSNIVLRANNKVKKANILARVQLPCQPNETLGDL
                     LVRHLNDEEKDVAIGSVVYINNVALRVDSMHDGLVTRVEIIRSLH"
     gene            993..>1231
                     /locus_tag="ACUBV3_001995"
     CDS             993..>1231
                     /locus_tag="ACUBV3_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000600818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3848462.1"
                     /translation="MILINLIALPICFLVELSAGLLLKLEMHKQHGYYPLERLLMTGN
                     ILTVVSLVLLHTLIHNINSLLFLSITVVLLISGKS"
BASE COUNT          434 a          221 c          175 g          401 t
ORIGIN      
        1 atcatgtatc ttaggataca tgatttttta tattttttaa tgtagactcc taatgatttc
       61 tacacgcgtc acaagaccat cgtgcatact atcaacacgc aatgcaacgt tattaatata
      121 gactactgaa ccaatagcaa cgtccttttc ctcatcattt aaatgacgaa caagtaagtc
      181 tcctaaagtt tcattgggtt gacaaggtag ttgaacccta gctaaaatat tagctttttt
      241 gactttgtta ttagcacgta aaacaatgtt gcttgaccca tcaaaatggg tgaaaagata
      301 tttacgaaca gcaaacaaaa tagcaactga agcaatcccc ataaggtaat ctgaaacact
      361 accatgttca attacaagtt gacgagctaa ggcaaacaat aacacttcaa ttaaagtatc
      421 tggggaaggt tttgaaaaca ttttgatcaa ctcaatcccg actgctattg tcatggcgcg
      481 agcaaggaaa gtctggaaca acttatcaac attttgatca acagacaaca tgctaaacaa
      541 atcatgaaag agtgctaaac taaaaaaaat aatagctgtt gccagaataa ctgacaaagc
      601 aatctctaaa taataagcca cctctacaat aacatcttgg aaaaatttac gcatacaatc
      661 tcctctttta taaactaata acattataac atttcgtcgt taaagttact aattcaatga
      721 aacaatagtg accacccctt cattaacctt ttaatcagta ctaacgaatg tttgattaca
      781 cattatgata taataagctt attggtaaat aagtgggagg aaatgaaata ttaattaata
      841 aacggttaag aacaatctcc ttactacatt atctgttagc gctactactt cttatagaca
      901 ttatctttac gattgatttt ataattcaca cgtcaacaat ttccaaaaat agtctatcat
      961 atataagtta tggtcaagga tttggttggt ctatgattct tattaatttg atagctctac
     1021 ctatttgttt cctagtagaa ttgtcagctg gtctactatt aaaattagaa atgcataagc
     1081 aacatggcta ctatcctctg gaacgcttat tgatgactgg taatattcta acagttgtat
     1141 cgctagtatt attacatact ttgattcaca atatcaattc acttcttttt ttatcaatta
     1201 cagtagtttt attgatttct gggaagtcct t
//
LOCUS       DBJYSH010000200         1216 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.200, whole genome shotgun sequence.
ACCESSION   DBJYSH010000200 DBJYSH010000000
VERSION     DBJYSH010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1216
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.200"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            complement(<1..379)
                     /locus_tag="ACUBV3_001996"
     CDS             complement(<1..379)
                     /locus_tag="ACUBV3_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HHU3848463.1"
                     /translation="MKNFIKQSDTTFVRIIKSLLIGGFIGAILGSVGALFNIFGQDKY
                     LSEINIVQYFLWVSRIVVIITALFSLIYLYQIQKYQKVFFNVDESQSEEIYRQINLRH
                     SYGMTFVSISIVLSIFNTLFNYKL"
     gene            complement(382..597)
                     /locus_tag="ACUBV3_001997"
     CDS             complement(382..597)
                     /locus_tag="ACUBV3_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HHU3848464.1"
                     /translation="MGLILKNRLKELRARDGINQTELAKLTGVSRQTISLIERGEYTP
                     SVIIAMKISQVFKESLENVFSLVSTEE"
     gene            complement(642..935)
                     /locus_tag="ACUBV3_001998"
     CDS             complement(642..935)
                     /locus_tag="ACUBV3_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3848465.1"
                     /translation="MVMTYIGVPKEQKALSRRGKQLEKINQGGKTLMSQMIQDLVGST
                     CMIYLEDGFQTHPYEILKVDEDWVQIRRNLKRSKEEVRILRIHDIKELRPVNK"
     gene            complement(967..1155)
                     /locus_tag="ACUBV3_001999"
     CDS             complement(967..1155)
                     /locus_tag="ACUBV3_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3848466.1"
                     /translation="MKKEFIITGRMVARVMETLVCLCMVSNLVLDMLDIIEINEIVFC
                     VLVASIAGLEFCLQKERE"
BASE COUNT          404 a          238 c          151 g          423 t
ORIGIN      
        1 ttagtttata gttaaataaa gtattaaata tactgagtac tattgaaatt gacacaaaag
       61 tcatcccata agaatgtctt aaattaatct ggcgataaat ttcttcagat tgactttcat
      121 caacattgaa aaaaactttc tgatattttt gaatctgata gaggtagatg agactaaaga
      181 gagctgttat aattacaaca atacgactaa cccataggaa atattgaaca atattaattt
      241 cagataagta cttatcttga ccaaatatgt taaaaagagc tcctacggaa ccaagtatgg
      301 cccctataaa accaccaatt aaaagacttt tgataattcg aacaaatgta gtatcacttt
      361 gttttataaa atttttcatc gttattcctc cgttgacact aaactaaaaa cattctctag
      421 ggattcttta aatacttgag aaatcttcat agcaataata acagatggcg tatattctcc
      481 gcgttcaatt aggctaattg tttgtcttga gactcccgtt aatttggcga gttctgtttg
      541 attgatacca tctcgtgcac gcaattcttt gagcctgttt tttaaaatta atcccatcat
      601 ttttctcctt tataaaatga tatatagatt tattcctcac tctatttatt gactggacgc
      661 aattccttga tatcatgaat acggagaata cgtacctcct ctttacttcg tttcaagttg
      721 cgacgaatct gtacccaatc ctcatctacc tttaaaatct cataaggatg cgtttgaaaa
      781 ccatcctcta aataaatcat acatgtgctt cctactaaat cttgaatcat ttgtgacata
      841 agtgtttttc ctccctgatt tattttttct aattgtttac ccctacgact gagtgccttt
      901 tgctctttag gtacaccaat atacgtcatg accataaaaa gccattaagc caaaaattcc
      961 atctatctac tccctttctt tttgcaaaca aaactcaagc cctgcaattg atgctaccaa
     1021 cacacaaaat acaatttcat ttatttctat tatatcaagc atatcaagaa caagattact
     1081 taccatacat aagcaaacta gggtttccat aactcttgca accatccgac ctgtaataat
     1141 aaattccttt ttcatttgag taactcctaa aaccattatg cttttgaaac aatgattaat
     1201 ttgattgttc aatgat
//
LOCUS       DBJYSH010000201         1203 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.201, whole genome shotgun sequence.
ACCESSION   DBJYSH010000201 DBJYSH010000000
VERSION     DBJYSH010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1203
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.201"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            complement(93..458)
                     /gene="rplL"
                     /locus_tag="ACUBV3_002000"
     CDS             complement(93..458)
                     /gene="rplL"
                     /locus_tag="ACUBV3_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HHU3848467.1"
                     /translation="MALNIENIIAEIKEATILELNDLVKAIEEEFGVTAAAPVAAAAA
                     GGEAAAAKDSFDVELTAAGDKKVGVIKVVREITGEGLKEAKAIVDNAPSVIKEGASEA
                     EANEIKEKLEAAGASVTLK"
     gene            complement(522..1022)
                     /gene="rplJ"
                     /locus_tag="ACUBV3_002001"
     CDS             complement(522..1022)
                     /gene="rplJ"
                     /locus_tag="ACUBV3_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HHU3848468.1"
                     /translation="MSEAIIAKKAEQVELIAEKMKAAASIVVVDSRGLTVEQDTNLRR
                     SLRESDVEFKVIKNSILTRAAEKAGLEDLKELFVGPSAVAFSNEDVIAPAKVISDFAK
                     DAEALEIKGGSVDGKFTSVEEINALAKLPNKEGMLSMLLSVLQAPVRNIAYAVKAVAE
                     KDEEVA"
     misc_feature    complement(1052..1185)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
BASE COUNT          349 a          246 c          197 g          411 t
ORIGIN      
        1 agattcaatt gaatctttcc ttttttaatc gccttaactt taacgaaaag actttaaaac
       61 taggctttca cctttaaaca accaatagat gattatttaa gagtaactga agcacctgca
      121 gcttcaagtt tttctttgat ttcgttagct tctgcttctg aagcaccttc tttgataact
      181 gatggtgcgt tatcaacgat agctttagct tctttaagac cttcgcctgt gatttcacga
      241 acaactttga taacgccaac ttttttgtct ccagcagctg ttaattcaac gtcaaatgag
      301 tctttagcag cagcagcttc accaccagca gcggcagcag ctacaggagc agcagcagtt
      361 acaccaaatt cttcttcgat agcttttaca agatcgttaa gttcaagaat tgtagcttct
      421 ttaatttcag caataatatt ttcaatgttt aatgccattg ttatttcctc caaaaatatg
      481 ttttaatata aaattatgta gctaggctac ttgaagagtg cttatgccac ttcttcgtct
      541 ttttctgcaa ctgctttgac agcatatgca atgttgcgta ctggcgcttg aagtactgaa
      601 aggagcattg aaagcatacc ttctttgttt ggtaatttag caagagcgtt aatttcttca
      661 actgaagtga atttaccgtc tactgaacca cctttgattt caagagcttc tgcatcttta
      721 gcgaaatcgc taataacttt agctggtgca ataacatctt cgttagaaaa cgcaactgct
      781 gatggaccaa caaaaagttc tttaaggtct tcaagacctg ctttttcagc tgcacgagtt
      841 aaaattgagt ttttgataac tttaaactca acatcgcttt cacgaagtga acgacgtaag
      901 ttagtatctt gctcaacagt aagaccacgt gaatcaacaa caacgatact tgctgctgct
      961 ttcattttct cagcaataag ttcaacttgt tcagcttttt ttgcaataat tgcttcactc
     1021 attagtttta cctccgtttt tattttttgg gataggcaca aaaaaatcgc acctaaacct
     1081 agacacgaaa gtacaatacg ttattttata atgacgtttt gtgcctcggt aggaatatta
     1141 tgagcactgc tcccctactg tcttaggtca gtttcatcag aaactttaaa tagtataact
     1201 tat
//
LOCUS       DBJYSH010000202         1172 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.203, whole genome shotgun sequence.
ACCESSION   DBJYSH010000202 DBJYSH010000000
VERSION     DBJYSH010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1172
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.203"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            67..1131
                     /locus_tag="ACUBV3_002002"
     CDS             67..1131
                     /locus_tag="ACUBV3_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HHU3848469.1"
                     /translation="MKRKQFIKLGIATLLTVISLYTPINLATNHTTENIVTAQEYKTK
                     ENGTLPFKHKRQLVLGELDDKGRATFAHIQLKVKDEPKKKRVKRLKTTPVGWHNFKFY
                     YNDGTQKAWLMSRGRLICHQFSGLNNERKNLVPMTNWLNTGNYNSTNSSNPESMLFYE
                     KQLKTWLSTHKNYYLDYKVTPIYQNNELIPRKIELKYVGIDKTGKLLPIFIGNKSTQD
                     QFGISTVTLENTSPNATIDYLSGKAQNTVLSAKEQRKLIAKHEEEKRLAEKKVEEEKA
                     AAETQKKLEEEQARLAAEAQRKQKEEQARLAAETQKKQETLVQEQTSQGYKRDYRGRW
                     HRPNGQYASKAEIAAAGLQW"
BASE COUNT          472 a          204 c          203 g          293 t
ORIGIN      
        1 ttttaatttt cagaatgcat ttaaaaataa acagctacta ttttatgtta ttttagaggt
       61 taacagatga aaagaaaaca gtttataaaa ttaggaattg caaccttact aacggttatt
      121 tcgctttaca caccaataaa cctagctaca aatcatacca cagaaaatat tgttactgct
      181 caagagtata aaacaaaaga gaatggtact ttacctttta aacacaaacg acaattggtt
      241 ttaggagagt tggatgacaa aggtcgtgcc acattcgcac atattcagct aaaagttaaa
      301 gatgaaccca agaaaaagag agtaaaacgt ttaaaaacaa cccctgttgg atggcacaat
      361 tttaaattct attataatga tggtactcaa aaagcttggc taatgagccg aggccgtctt
      421 atttgccacc aatttagtgg actaaacaat gaaagaaaga atttagttcc aatgacgaat
      481 tggctaaata ctggaaacta caatagcacg aattcatcta accctgagag catgctattc
      541 tatgaaaaac aactaaagac ttggctttcg actcataaaa actactactt agattataaa
      601 gtaacaccaa tttatcaaaa taatgaacta attccaagaa aaattgaatt aaaatatgtt
      661 ggtattgata agactgggaa attattacct atttttatcg gaaacaaatc cactcaagat
      721 caattcggca tcagcactgt taccttagag aatacctcac caaatgctac cattgactac
      781 ctcagcggaa aggctcaaaa tactgtctta tcagctaaag aacaaagaaa acttatcgct
      841 aagcacgaag aagaaaaacg acttgcagaa aagaaagttg aagaggaaaa agctgctgct
      901 gaaactcaga aaaaactgga agaagaacaa gcaaggcttg ctgctgaagc tcaaaggaag
      961 caaaaagagg agcaagcaag gctcgcagct gaaactcaga aaaaacaaga aactctagta
     1021 caagaacaaa ctagtcaagg ctacaaacgt gactatagag gaaggtggca tcgtccaaat
     1081 ggacaatacg cttcaaaagc agaaatcgct gctgctggat tgcaatggta gaactgttta
     1141 ttatcaaatc aattctatta ttataatgtg ta
//
LOCUS       DBJYSH010000203         1144 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.204, whole genome shotgun sequence.
ACCESSION   DBJYSH010000203 DBJYSH010000000
VERSION     DBJYSH010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1144
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.204"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            complement(12..995)
                     /gene="guaC"
                     /locus_tag="ACUBV3_002003"
     CDS             complement(12..995)
                     /gene="guaC"
                     /locus_tag="ACUBV3_002003"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HHU3848470.1"
                     /translation="MFNDIPVFDYEDIQLIPNKCIISSRSQADTSVKLGNYTFKLPVI
                     PANMQTIIDEEVAETLACEGYFYIMHRFNEEARRPFIKRMHDKGLIASISVGVKDYEY
                     DFVTSLKEDAPEFITIDIAHGHSNSVIEMIQHIKQELPGTFVIAGNVGTPEAVRELEN
                     AGADATKVGIGPGKVCITKVKTGFGTGGWQLAALRWCSKAARKPIIADGGIRTHGDIA
                     KSIRFGASMVMIGSLFAGHLESPGKLVEVEGQQFKEYYGSASEYQKGEHKNVEGKKIL
                     LPVKGRLEDTLTEMQQDLQSSISYAGGKELDSLRHVDYVIVKNSIWNGDSI"
BASE COUNT          358 a          240 c          163 g          383 t
ORIGIN      
        1 caagatttaa tttaaatgga atcaccattc caaatagaat ttttaacaat aacgtaatca
       61 acgtgtctca agctatccaa ttcttttcca cctgcgtaag aaatagaact ttgcaaatct
      121 tgttgcattt ctgtcaaagt atcctctagt cgccctttaa caggtaatag aattttctta
      181 ccttcaacat ttttatgttc tcccttttga tattcagaag ctgagccata gtattcttta
      241 aattgctgtc cttctacttc cacaagtttt ccaggacttt caaggtgacc agcaaacagc
      301 gaaccaatca ttaccatact agcaccaaag cggatagatt ttgcaatatc cccatgtgtt
      361 cgaataccac catcagcaat aattggcttg cgagcagctt tagaacacca acgtaaggca
      421 gctaattgcc aacctccagt accaaaacct gtcttgactt tagtaataca aactttacca
      481 ggtccaatac caactttagt tgcatctgca cctgcatttt ccaattctct aacagcttca
      541 ggcgttccta catttccagc aataacgaaa gttcctggaa gctcttgttt aatgtgttgg
      601 atcatctcta taacactgtt agaatggcca tgtgcaatat caatagtgat aaactcagga
      661 gcatcttctt tcagacttgt cacaaaatca tattcatagt ctttgacccc aaccgaaatc
      721 gaagcaatta agcctttatc atgcatacgc ttgataaaag gtcttcttgc ttcttcatta
      781 aaacggtgca taatataaaa ataaccttca cacgctaatg tttcagctac ctcttcatca
      841 ataatagtct gcatattagc agggattact ggaagtttaa aggtatagtt tccaagtttg
      901 acacttgtgt cagcttgtga gcggctagat ataatacact tgtttggtat taattgaata
      961 tcttcataat caaaaacagg aatatcgtta aacataatgg ttctcctaat gagtaaaatg
     1021 accttcctat aactttgtat aacaaagcat cgctaggtaa aaacatacct ttccttttta
     1081 aattatatac tttatttatt tttagtcaat ttttgatcaa tttatttgaa taatgttcgt
     1141 tttt
//
LOCUS       DBJYSH010000204         1142 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.205, whole genome shotgun sequence.
ACCESSION   DBJYSH010000204 DBJYSH010000000
VERSION     DBJYSH010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1142
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.205"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            62..1054
                     /locus_tag="ACUBV3_002004"
     CDS             62..1054
                     /locus_tag="ACUBV3_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HHU3848471.1"
                     /translation="MKLKVFNVREEEATLAQDWANRNHVELSMSEGPLTLETVNEVEG
                     FDGIANAQIEPLDDAIYPLLKEMGIKQIAQRSAGVDMYNLELAKQHGIIISNVPSYSP
                     ESIAEFTVTIALNLIRKVELIRANVREQNFSWTLPIRGRVLGNMTVAIIGTGRIGLAT
                     AKIFKGFGCRVIGYDIYHNPMADGILEYVNSVEEAVEEADLVSLHMPPTAENTHLFNL
                     DMFKQFKKGAILMNMARGALVETKDLLEALDQGLLEGAGIDTYEFEGPYIPKNCQGQD
                     ISDKDFLRLINHPKVIYTPHAAYYTDEAVKNLVEGALNACVEVIETGTTTTKVN"
BASE COUNT          375 a          170 c          248 g          349 t
ORIGIN      
        1 aaagtactta aaattattga tccaatggac tataaattag atatttaagg agatattagt
       61 gatgaaactc aaagtattta atgttagaga agaagaagca acattagcac aagattgggc
      121 aaaccgaaat catgtggaac tttctatgag tgaaggacct ttgacattgg aaactgttaa
      181 tgaggtagaa ggttttgatg ggattgctaa tgcacagatt gagcctttag atgatgctat
      241 ttatcctctt ctcaaagaaa tgggaattaa gcaaatcgct cagcgcagtg ctggtgttga
      301 tatgtataat cttgagttag ctaagcaaca tggtattatc attagcaatg tgccaagtta
      361 ctcaccggag tctattgcag aatttacagt gacaatcgct cttaacctta ttcgaaaagt
      421 tgagcttatt agagctaatg ttcgtgaaca gaatttttca tggaccttgc caattcgagg
      481 acgagtccta ggtaacatga ctgtggctat aataggaact ggtcgcattg gtctagcgac
      541 tgctaaaatt tttaaagggt tcggatgtcg tgttattggt tatgatattt accataatcc
      601 aatggcagat ggtatccttg agtatgtcaa ttcggtagaa gaagctgttg aagaagctga
      661 tcttgtgtcc ttacatatgc caccaactgc tgaaaatacg catctattta atttagatat
      721 gtttaaacag tttaaaaaag gtgctatttt aatgaatatg gcacgtggag cattggtgga
      781 aaccaaagat cttcttgagg ctttagatca agggcttttg gagggagctg gtattgatac
      841 ttatgaattt gaggggccat atatacctaa aaattgtcaa ggtcaagaca tttcagacaa
      901 ggatttccta agactgatta accatccgaa agtaatatac actcctcatg cggcttatta
      961 tacagatgaa gcggtcaaaa atctagtaga gggtgcctta aatgcttgtg tagaagtgat
     1021 tgaaacggga acaactacta cgaaagtcaa ctaaaagatg cttaggcatc tttttttgtg
     1081 gggtaacaaa acatatgatg attatgacct ttaaagcaaa aaaacatctt atgtatttat
     1141 ag
//
LOCUS       DBJYSH010000205         1141 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.206, whole genome shotgun sequence.
ACCESSION   DBJYSH010000205 DBJYSH010000000
VERSION     DBJYSH010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1141
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.206"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            85..525
                     /locus_tag="ACUBV3_002005"
     CDS             85..525
                     /locus_tag="ACUBV3_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HHU3848472.1"
                     /translation="MENPLQKARILVNQLEKYLDHYAKEYDVEHLAGPQGHLVMYLYK
                     HPDKDMSIKAVEEILHISKSVASNLVKRMEKNGFIAIVPSKTDKRVKYLYLTHLGKKK
                     ATQFEIFLEKLHSTMLAGITKEEIRTTKKVIRTLAKNMAMENLD"
BASE COUNT          410 a          142 c          188 g          401 t
ORIGIN      
        1 tagttcttac gataagtttt aattgacaat taataaaggt tgttttaaaa tagttctcac
       61 ggtaactatt aaaggagtat gattatggag aatcctcttc aaaaagcacg aatacttgtt
      121 aatcaacttg aaaagtattt agatcattat gcaaaagaat atgatgttga acatttagcg
      181 ggtccacaag gacacctagt gatgtatctc tataaacatc ctgataagga tatgtctata
      241 aaagctgttg aagaaatctt acacatttct aagtctgtag cttctaattt ggtaaaacgg
      301 atggaaaaaa atggttttat tgcgattgta ccatctaaga cagataagcg tgtaaaatat
      361 ctttatttga ctcacttagg taaaaaaaag gctacacaat ttgaaatttt cttagaaaaa
      421 ttgcatagta ctatgttagc aggtattact aaagaagaga ttcgtaccac caaaaaggtt
      481 attaggacat tagctaaaaa tatggcaatg gaaaatcttg attaactatc ttagggtgat
      541 gacttaatgc tgtgatttta ttaaagacat gaagagagaa ttggtaagaa ttacttctac
      601 aggattgcta gtttaacaat atttaataaa ggtaagttat ctagagaatc atttttaaat
      661 atagccgata gttctcgttt accatttcaa cttcaatcta aaagaactaa acgattagtc
      721 actttaactg ttttgttctt ttggaaattc ctgtgtttaa gtagttttag tgttaaagag
      781 caacatgatt gatgaggatg atattgtaaa gaaattttag gaaattttta agctacagtt
      841 taacttgtta tactgtaata ctttgagatg aacctcccat ttggacaatg agcaaggtaa
      901 atagatatac taaatatttt aatacaatca gctacaccag atagttttta tagtgtctga
      961 taatatgatt ttaaacctgc ttaatagact tgtgataata taatcaattg taaatagctt
     1021 tcagagacac tttttagtta aaagtctcct taattatctt tatattggat agaagtatcg
     1081 aatctgtttt atgtaaacta ccaggtctta gtatctattt ttcaatttat cttgaataag
     1141 a
//
LOCUS       DBJYSH010000206         1140 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.207, whole genome shotgun sequence.
ACCESSION   DBJYSH010000206 DBJYSH010000000
VERSION     DBJYSH010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1140
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.207"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            complement(67..321)
                     /locus_tag="ACUBV3_002006"
     CDS             complement(67..321)
                     /locus_tag="ACUBV3_002006"
                     /EC_number="3.4.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001923489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HHU3848473.1"
                     /translation="MFVMRTFGNSLSGPLVVILSSILFSWSHLHGLSVVDFVVYFGMG
                     LIFASLHHYTKSIHYSIGEHIVWNSLSYIFYFLAFLLDLL"
     gene            complement(340..591)
                     /locus_tag="ACUBV3_002007"
     CDS             complement(340..591)
                     /locus_tag="ACUBV3_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000973012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3848474.1"
                     /translation="MMILLSYVLTKTEPISNYQESSSNLKKWLFAIGAFSVLFCWALL
                     VKTFFPIRQNQEGLIADMNQVNHYFRVVLLLTICLIAPI"
BASE COUNT          429 a          200 c          178 g          333 t
ORIGIN      
        1 gagccaaaaa gctcttttaa actgacaaaa atgtcttagt aatgattagt ctctcaatct
       61 tattacttag agtaaatcaa gcaaaaatgc tagaaaataa aaaatatatg acaaactatt
      121 ccaaacaata tgctcgccaa tcgaataatg aatggatttt gtatagtggt gcaaactagc
      181 gaaaatcaaa cccataccaa aatagacaac aaagtcgacg acagatagac catgtaagtg
      241 agaccagcta aatagtatag aagataagat cacaaccaag ggaccactca gggagttccc
      301 aaatgttctc atgacaaaca acctgaacgt tatctcttct taaattgggg caataagaca
      361 gatagtcaat aataagacaa ctcgaaagta gtgattgacc tgattcatat cagctattaa
      421 tccctcctgg ttttgcctta ttgggaaaaa tgtttttact agtagtgccc agcaaaataa
      481 aacgctaaaa gcacctattg caaatagcca ttttttgagg ttagatgatg actcttggta
      541 gttactgatt ggctcagttt ttgttagcac ataactcaac agaatcatca ttataagatt
      601 aatgccaatc catatactcc aactccactc taaccctatt gatagtaaac taatcaccca
      661 atgcccaagc aattgttatt attgtgttgt tgcaactttg gcaaagatga atatagcagg
      721 atggaaaaca gattgtttct gaaacttcaa aggtatcttc ttatcacaag ctattatttg
      781 gttatctcca taattaatct aaatggagca atatatcgtt tttctactaa ctgttgacaa
      841 ataaaaaact cccctttaaa gtctggtaac ttttcgattt ttaccagaaa cttaaggaga
      901 gtgaggcaag aactaataga atcttattta aagttttatg attgggagaa cagaacaact
      961 atcataatta ctgaaaaatt atgaaaggca tggcaaacaa cagatggtca aatccgctct
     1021 gttgaaaaca aattttaaat ataattcatt tcactttcta agtttattaa acatagagtg
     1081 ttcaggagaa atgatacgac ctaatgataa aatcattcct atcgtataat aaacaacgac
//
LOCUS       DBJYSH010000207         1118 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.209, whole genome shotgun sequence.
ACCESSION   DBJYSH010000207 DBJYSH010000000
VERSION     DBJYSH010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1118
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.209"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            242..634
                     /locus_tag="ACUBV3_002008"
     CDS             242..634
                     /locus_tag="ACUBV3_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001191783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2785 domain-containing protein"
                     /protein_id="HHU3848475.1"
                     /translation="MQSQKLNVLQRVLRDKFLLQGLGVPNSNTVLNRSFTALLLALLL
                     EDSNNKSWISEEDQIKIMNQAFSWLIDEPDFRGFDEELGWIHAFAHGADLLTEIVKMK
                     CFSKDDSLKILTIIKRVMITDDILTLNG"
     gene            785..>1118
                     /locus_tag="ACUBV3_002009"
     CDS             785..>1118
                     /locus_tag="ACUBV3_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /protein_id="HHU3848476.1"
                     /translation="MRDEQEIYNLVLNIANQDKRIEAVLLNGSRANPNVPKDDFQDYD
                     IVFVTNFIEDIISDTNYHKKFGDILIMQKPNEFRNKTEYNCFAYLMQFQDLTRIDLRL
                     IKPEFLEDY"
BASE COUNT          429 a          130 c          211 g          348 t
ORIGIN      
        1 ctataatcat tttaaagtga aaacgaaaac tcaaatgtta cactttggaa aatataaaat
       61 ataaagtcat gcaaggagaa acatggaagc aagtgatgta atcaaaaaga tagaaaataa
      121 ttatccaatt gatgaggatg ttttatcttt ttgattacat catataggag attggaaccc
      181 aagagttcga gatgaaatag tatatgggtc ttgggttaaa ttagtaatag aacataaact
      241 gatgcaaagc cagaaattaa atgtccttca gagagtttta agggataaat ttcttttaca
      301 aggattagga gtacccaaca gtaatactgt gttaaacaga tcttttactg ctctattact
      361 agctttatta ttggaggact caaataataa aagttggata tctgaagaag atcagataaa
      421 aattatgaat caggcattct cctggcttat agatgaacct gattttagag gctttgacga
      481 ggagcttgga tggatacatg cctttgctca tggagctgat ttattaacag aaatagtcaa
      541 gatgaaatgt ttttcaaaag atgatagttt aaaaatctta actatcataa aaagagtgat
      601 gataactgat gacatactaa cattaaacgg ttgaaaatat ttggaatcat aatcagttgt
      661 taagaaaaat ttaaaattga atccttggag gtcgtaaagg tatgtccatg ctaatttaag
      721 tctatgatta catgcttaga gaaaaataca agggtaatat aaagtgaagt aggaggtagc
      781 cattatgaga gatgagcaag aaatatataa tttggtgtta aacatagcaa accaagataa
      841 gagaatcgaa gctgtactat tgaatggatc tcgggctaac ccaaatgtac cgaaagatga
      901 ttttcaagat tatgatattg tttttgttac taattttata gaagatatta tttctgacac
      961 taattatcat aaaaaattcg gtgatattct tataatgcag aaaccaaatg agtttagaaa
     1021 taagacggaa tacaattgct ttgcttactt aatgcagttt caagatctga cgaggattga
     1081 tttaagactt attaaaccag aattcttgga ggattatt
//
LOCUS       DBJYSH010000208         1071 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.210, whole genome shotgun sequence.
ACCESSION   DBJYSH010000208 DBJYSH010000000
VERSION     DBJYSH010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1071
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.210"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            78..1040
                     /locus_tag="ACUBV3_002010"
     CDS             78..1040
                     /locus_tag="ACUBV3_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017766755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HHU3848477.1"
                     /translation="MSIKKLLFAGLAFILFTLASPAYAASDVQKVIDETYVQPDYVLG
                     YSLNQEQRAQTLQLLNYDESRDTKVKTLNTSSYAKIMNIADDASIQLYSSVKIKKLGS
                     NDTLAVNIVTPENITKVTEDMYRNAAVTLGIEHATISVAAPIKVTGESALAGIYYSLE
                     KNGASVSSENKQLAQEELSTLSGINAENKGKEGYDADKLNVALTDIKSAVAKGGSDLS
                     KDDIRKIVEETLKNYHLDNAVTENQINLIVNFAVNLSQSNVIKNSDFTNTLNNLKDNI
                     VSKAGSKFKNINVNFNANKAVESGKGFLANIWQQIVNFFQQFGS"
BASE COUNT          401 a          143 c          196 g          331 t
ORIGIN      
        1 ataagtaagt taatcatggt ataatgtaag gtatgaaaag aattgaaaag gaattatttt
       61 gaaatgagga tactaaaatg agtattaaaa agctattgtt tgcaggcctt gcttttattt
      121 tatttaccct agcgagtcca gcatatgctg catcagatgt tcagaaagtt attgatgaaa
      181 cttatgttca acctgattat gtcttaggtt attcgctaaa tcaagaacag cgtgcacaga
      241 cattacaact tctgaattat gatgaatcta gagacacgaa agttaaaacg ctaaatacta
      301 gctcatatgc taaaataatg aatatagctg atgatgcaag catccaatta tattcatctg
      361 ttaaaataaa aaaattaggg tctaacgata ccttagcagt aaacattgtt acacctgaga
      421 atattactaa agttacagaa gatatgtatc gcaatgctgc agttacatta ggtattgagc
      481 atgcaacgat atcagtggct gctccaataa aagtaactgg ggagagtgct cttgcaggta
      541 tctattattc attagagaaa aatggagcaa gtgtatcatc agaaaataaa caactagcac
      601 aagaagagtt aagtacttta tctggtatta atgctgagaa taagggtaag gaaggttatg
      661 acgccgacaa gcttaatgta gctttaacgg atattaagtc agccgtagct aaaggaggat
      721 ctgatctttc aaaagatgat attcgaaaaa ttgttgagga aacattgaaa aattatcatc
      781 ttgataacgc tgtaacggaa aatcaaataa atttaattgt taattttgca gttaatcttt
      841 cacaaagtaa tgtcatcaaa aatagtgatt tcacgaacac cttgaataat ttgaaagaca
      901 acattgtttc gaaagcagga tctaaattta aaaatatcaa tgtcaatttt aatgctaata
      961 aagctgttga atcaggtaag ggatttttag ctaatatttg gcaacaaatt gttaattttt
     1021 tccagcagtt tggtagttaa taaaaaagtc tagcttataa gctagacttt t
//
LOCUS       DBJYSH010000209         1035 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.211, whole genome shotgun sequence.
ACCESSION   DBJYSH010000209 DBJYSH010000000
VERSION     DBJYSH010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.211"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            complement(35..943)
                     /locus_tag="ACUBV3_002011"
     CDS             complement(35..943)
                     /locus_tag="ACUBV3_002011"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HHU3848478.1"
                     /translation="MKTIKDWQINICLATYNGQKYLRQQLDSIIQQGYTDWICLIRDD
                     GSTDDTVAIIKEYVNRDSRFIFINSNDDRKLGSHRSFYELVNYKKADFYVFSDQDDVW
                     KENRLERYLEEAEKFNQELPLLVYSNWTSVDEKLTVLKEHNPATVIQEQIAFNQINGM
                     VIMMNHELAKLWEYRQIGAHDSYVGTLAYAVGNVAYISDSTVLWRRQVGAESLNNYGR
                     QYGVATFWQMINTSFDRASLIFAQVSDKMSLERKLFFSRFIELKNANLIRRIYLLSKL
                     KLRRKSLKETVAMTILLLTGYGKPKA"
BASE COUNT          333 a          199 c          149 g          354 t
ORIGIN      
        1 tacaactcaa cttgatttcc ttcaaccaaa aaaattatgc ttttggttta ccgtaacctg
       61 ttaaaagtag gatagtcata gcaactgttt ctttcaatga tttccgacga agttttaatt
      121 ttgataaaag ataaatcctt cttattagat tggcattttt taattctata aaacgactaa
      181 agaagagttt acgctccagg gacattttat cggatacttg agcaaaaata agcgaagcac
      241 gatcaaaaga agtatttatc atctgccaaa acgtagccac cccatattgt ctcccgtaat
      301 tgtttagcga ctctgcccct acttgtctac gccaaaggac tgtactgtca gaaatataag
      361 ccacatttcc aacggcgtaa gccagagttc caacataaga atcatgggcg ccaatttgac
      421 gatattccca aagctttgct aactcatgat tcatcatgat aaccatacca tttatttggt
      481 taaaggcaat ttgttcttga ataactgttg ctggattatg ttctttcaat actgttaatt
      541 tttcatctac agaagtccag ttcgaataaa caagcaatgg taattcttgg ttaaatttct
      601 ctgcttcttc aaggtaacgt tctaaacgat tttctttcca aacatcatcc tggtctgaga
      661 aaacataaaa atctgctttt ttatagttaa ccaactcgta aaaactgcgg tgacttccta
      721 actttctgtc atcgttagaa ttaataaata taaaacgtga atctctatta acatattctt
      781 taataatagc tactgtatca tcagtagatc catcgtccct tattaaacaa atccagtcag
      841 tataaccttg ttgtattatg ctatctaatt gctggcgaag gtatttttga ccattatagg
      901 tcgctaagca aatatttatt tgccaatctt ttatcgtttt cattgctatc aaaactcctt
      961 aaggctagtt catcttatga aagcttacaa agtgaacggt atcacatact aatcgtaatt
     1021 ataatattat aatat
//
LOCUS       DBJYSH010000210          946 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.212, whole genome shotgun sequence.
ACCESSION   DBJYSH010000210 DBJYSH010000000
VERSION     DBJYSH010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..946
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.212"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            90..620
                     /locus_tag="ACUBV3_002012"
     CDS             90..620
                     /locus_tag="ACUBV3_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHU3848479.1"
                     /translation="MSPSEKLDRRIDSSSDLSQYVYDFEKQYTEYMKKGYSEKNASIQ
                     ARRTLNEILQENNPGLRSSDAMYDKETGIAAIAVKDKNTGETYIAYAGTNKDADGATD
                     FISDANIGANNSAYLKRLGDKATSFYDRVQSNGDNITVTTGHSYGDFLASRVAIELIP
                     FFINFRKVNDSFHFCY"
     gene            712..893
                     /locus_tag="ACUBV3_002013"
                     /pseudo
     CDS             712..893
                     /locus_tag="ACUBV3_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395449.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          336 a          138 c          179 g          293 t
ORIGIN      
        1 tattattata caatcagtat tgatactgat tctgagatat attctatatt cgaaggtatt
       61 acattagagg atgtaaagga gttttctaaa tgagtccaag tgagaaatta gatagaagaa
      121 ttgattcttc atcagacctc tcccagtatg tatatgattt tgaaaagcaa tatacagaat
      181 atatgaagaa ggggtattct gaaaaaaatg cgagtataca agcacgacga accctgaatg
      241 aaatactaca agaaaataat ccaggattga gatctagcga cgccatgtat gataaagaaa
      301 caggtattgc agctattgct gtaaaagata aaaatactgg tgagacttat atagcttatg
      361 cgggtactaa taaggatgct gatggggcaa ccgactttat ttcagatgca aatatcggtg
      421 ctaataactc tgcttattta aaacgattag gagacaaagc tactagcttt tatgatcgtg
      481 ttcaaagtaa tggcgataat attactgtca caacaggtca ttcttatggt gactttctcg
      541 cttctagagt ggcaattgag ctaattccat tctttattaa ctttagaaaa gttaatgatt
      601 catttcactt ttgttattag cttgtgatca cttataaagt gacagctagt tcatgaaatt
      661 tcttaggagt tttctggctt taaaggtaac ctatgattta aaaaacatct catggaattc
      721 aatgcggaat cctcaacagt cttttgtgga ctaacagaag ttcaggatgt cgtttcggca
      781 ggccttaact attctcagaa ggttttggaa atttcaatgg tacatttgat acctcaggta
      841 agggattaga atggacaaaa acaattcaaa caggtatgaa aaaacgagaa gagaaaatca
      901 aataccctca ttttacaatt gttgacataa agtaattaaa caaaat
//
LOCUS       DBJYSH010000211          909 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.213, whole genome shotgun sequence.
ACCESSION   DBJYSH010000211 DBJYSH010000000
VERSION     DBJYSH010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..909
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.213"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            1..240
                     /locus_tag="ACUBV3_002014"
     CDS             1..240
                     /locus_tag="ACUBV3_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001869992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HHU3848480.1"
                     /translation="MTLFLNSLLKELIIVNRLREYRIKASISQLALAKSVGVARQTIN
                     LIENNKYNPSLDLCIRLAECLQTDLNTLFWDSRHL"
     gene            340..873
                     /locus_tag="ACUBV3_002015"
     CDS             340..873
                     /locus_tag="ACUBV3_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3278 domain-containing protein"
                     /protein_id="HHU3848481.1"
                     /translation="MKEKKNLGIKLIKSLYGINGPFDEYREQKINYLGNLLFATTSLY
                     HILSSIIAWFTVSKYPTETACTLLALTFFLYFCIFLIVIPSYLNSHDLITPLEVSKND
                     YPVAIKKNKQKFIKIGIFYTVIQWLILGLTTNNNTPLIVSLFSLKVILSSIMSGVAYN
                     GVMYLIFKNNVDIEKDE"
BASE COUNT          324 a          156 c          126 g          303 t
ORIGIN      
        1 atgacacttt tcttaaattc tttactaaag gagctaataa tcgtgaatcg tcttagagaa
       61 tacagaatta aagctagcat atctcaatta gctttagcaa aatcagttgg tgtcgccaga
      121 caaacaataa accttattga aaataataaa tacaatcctt cgctagatct ttgtataaga
      181 ctagcggaat gcctacaaac agacctcaat actctttttt gggatagtag acatctataa
      241 cagattagac tctctaaatc tatgaaaact cgggaggtta ggtcgtatta ttatttcaat
      301 ctcaaacttt attaaatagt tcaaggatag gagaatatta tgaaagaaaa gaaaaatttg
      361 gggataaaat taataaaatc cctatatggt attaatggcc cattcgatga atacagagaa
      421 caaaaaatca attaccttgg aaatctatta tttgcaacca ctagtctcta tcacatttta
      481 tcctctatca tcgcctggtt cacggtaagt aaatatccca cagagactgc atgtactctc
      541 ctagctttaa cattctttct atatttttgt atttttctaa ttgttattcc ctcttatctc
      601 aacagtcatg atttaataac gccactagaa gtctcaaaaa atgactatcc agtagcaata
      661 aagaaaaaca aacaaaaatt tatcaaaatc gggatatttt atactgtcat ccagtggctt
      721 atcttgggtt taacaactaa caataacact ccgctgattg tatcactatt ttctttaaaa
      781 gttattttgt ctagtattat gtccggtgta gcttacaatg gagttatgta tctaatattt
      841 aaaaacaatg ttgatattga aaaagatgaa taatgacaac atttcatctc taattgttct
      901 cactgtcta
//
LOCUS       DBJYSH010000212          906 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.214, whole genome shotgun sequence.
ACCESSION   DBJYSH010000212 DBJYSH010000000
VERSION     DBJYSH010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..906
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.214"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            <1..>906
                     /locus_tag="ACUBV3_002016"
     CDS             <1..>906
                     /locus_tag="ACUBV3_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sigma factor regulator N-terminal
                     domain-containing protein"
                     /protein_id="HHU3848482.1"
                     /translation="FSDPLEQLAKKRKRKTLFKTVVLALITVLLVVGIAFKGFTYLTS
                     KNGQKTYHNFELLSEIAYPNISYDSLYYQPSGQFTGKVHADRFKDIDGVQIPYSSFEE
                     NYSISGTFGTNADEKSEKNGLYDRGTRQKVPQFFNKNVKYKKGEAKTTPTNDLKYVNK
                     LNNDLIEIAITFDKSYSYKEIKTMIPNNIKQNWLWIGTSTELDTSYWPTKYQFGTDPE
                     TLNTPQIFIDKIKENLKGNSKVYFNNINIYSDLEKYAKKYDKVNNSDKLKFSGIILTG
                     KAENFKQLKEKEWVHASSIGANIEY"
BASE COUNT          398 a          121 c          136 g          251 t
ORIGIN      
        1 agtttagtga tccactggaa caattagcca aaaaaaggaa aaggaaaaca ttattcaaga
       61 cagtagtatt ggcattaata actgtactat tagtagttgg aatagctttt aaaggattta
      121 catatttaac ttcaaaaaat ggtcaaaaaa catatcataa ctttgaacta ctttctgaaa
      181 tagcatatcc aaatatttcc tatgatagtc tctattacca gccaagcggt cagtttactg
      241 gtaaagtaca tgctgataga tttaaagata tcgatggggt tcaaattcca tattctagtt
      301 ttgaagaaaa ttatagtatc tcaggtactt ttggcacaaa tgctgatgaa aaatcagaaa
      361 agaatggctt gtatgatcga ggtactagac aaaaagttcc acaatttttc aataaaaatg
      421 ttaagtataa aaaaggagaa gccaaaacaa ctccaacaaa tgatttaaaa tatgtaaata
      481 aattgaataa tgatcttatt gaaattgcaa taacttttga taagtcctat agttacaaag
      541 aaataaaaac aatgatacca aataatataa aacaaaattg gttatggata ggtacaagca
      601 cagaattaga tacatcatat tggccaacaa agtatcaatt cggtacagat cctgaaactt
      661 tgaatacacc acaaatattc atagataaaa taaaagaaaa cttaaaaggt aactcaaaag
      721 tatactttaa taatataaat atctactcag atttagaaaa gtatgccaaa aaatacgaca
      781 aggtaaataa tagcgacaaa ttaaaatttt cgggaataat attaacaggc aaagccgaaa
      841 actttaaaca acttaaagaa aaagaatggg tacatgcttc aagtataggg gcaaacatag
      901 aatata
//
LOCUS       DBJYSH010000213          905 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.215, whole genome shotgun sequence.
ACCESSION   DBJYSH010000213 DBJYSH010000000
VERSION     DBJYSH010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..905
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.215"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            79..837
                     /gene="cfb"
                     /locus_tag="ACUBV3_002017"
     CDS             79..837
                     /gene="cfb"
                     /locus_tag="ACUBV3_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001085574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAMP factor pore-forming toxin Cfb-II"
                     /protein_id="HHU3848483.1"
                     /translation="MAKKQVVYFSIAILTGVALFSPAILEVHADQATNHVVVSQVNNQ
                     QQIKQKLDQDSVQLNTIKNQVQGTDYEKPVDTAINAVDKMKSSLGGNPATIYDLGSIG
                     PRIEAITDVIEAINFSTHNLTNKVSQANVDMGFGITKLVIRIADPFASVDSIKAQVAE
                     VKQLEQKVMTYPDLKPTDKATIYTKSKLDKAIWDIRVQRDQKVLNKKSFDVYNALNKA
                     ITHAVGVQLNPNVTVQEVDQEIVNLQMALQTALK"
BASE COUNT          335 a          142 c          157 g          271 t
ORIGIN      
        1 gtatactact ggtgtaggta cctacaagtt aaaaatactg cagtatatta agttattttg
       61 atttaggagg gaaaaaccat ggcaaagaaa caagtagtct atttttccat agctattttg
      121 acaggagttg cattgttttc tccagcgatt ttggaagttc atgctgatca agctactaat
      181 catgtcgtag ttagtcaagt taataatcaa caacaaataa aacaaaaact tgatcaagat
      241 agtgttcaat tgaatactat taaaaatcaa gttcaaggca cagattatga aaaacctgta
      301 gatactgcta taaatgctgt tgataagatg aagtcttctt taggtggtaa tccagcgaca
      361 atctacgatc ttggttctat tggtccacgt atagaagcta tcacagatgt tattgaagca
      421 attaattttt caacacataa cttgactaac aaagttagtc aagctaatgt tgacatggga
      481 ttcggaataa ctaaattagt tattcgtatt gctgatccat ttgcatctgt tgattctata
      541 aaagcacaag tagcagaagt gaagcaatta gaacaaaaag taatgactta tccagatttg
      601 aagccaacag ataaagcaac tatttatact aagtcaaaac tggacaaagc aatttgggat
      661 attcgtgtac aaagagacca aaaagtttta aacaagaaaa gttttgatgt ttataatgct
      721 ttaaataaag ctatcacaca tgcagtaggc gtccaattaa atcctaatgt aaccgttcaa
      781 gaagtggatc aagagattgt gaacctacaa atggcacttc aaactgcttt gaaatgagtt
      841 gatactaaac cagaacgcga ttactactaa tctccggtcc gttcctatca aattaaataa
      901 taaat
//
LOCUS       DBJYSH010000214          882 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.216, whole genome shotgun sequence.
ACCESSION   DBJYSH010000214 DBJYSH010000000
VERSION     DBJYSH010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..882
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.216"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            complement(22..>882)
                     /locus_tag="ACUBV3_002018"
     CDS             complement(22..>882)
                     /locus_tag="ACUBV3_002018"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HHU3848484.1"
                     /translation="EETAKGKVSRHVLLTILLALILLFTADFLTYIIIELIISLDFWK
                     LEWDSDIVQLIILLATSISTVMLFMWVKFKEKRPISSLGLFKEGALTELSRGWLVGSV
                     LFGACLILTIISGSGVIETVRFTADKLIWFLIFAVGWQVQSASVEILCRGWLLPVSAT
                     KYSKIVSVSISSIFFGLLHSANNHVSLISIFNLCLFGLFLSLYVILKGNIWGACGIHG
                     AWNCVQGSVFGIEVSGEPMLSNSLVHVKTYGADWISGGKFGVEGSMITSIVLIVACYW
                     LYQKSNFPYD"
BASE COUNT          314 a          194 c          135 g          239 t
ORIGIN      
        1 agcttattta gattaaatct attagtcgta aggaaaattc gatttttgat aaagccaata
       61 acatgctacg ataagaacaa tactggtaat catgcttcct tccacaccaa attttccacc
      121 gctaatccag tcagcaccat aggttttaac atggactagg gagttagaca acattggctc
      181 tccactaact tcgattccaa agacactccc ttgtacacag ttccaggcgc catgaatacc
      241 acacgccccc cagatgttac cttttaaaat aacatagagc gatagaaata gtccaaagag
      301 acacagattg aaaatcgaga taaggcttac atgattatta gcgctatgca gaagaccgaa
      361 aaagattgaa ctgatactga cagaaactat cttactatac tttgtggcac tcaccggaag
      421 taaccatcca cgacacaata tttcaacaga tgcgctttgt acctgccatc caacagcaaa
      481 aataagaaac caaatcaatt tatcagctgt aaaacgaaca gtttcgataa ctccagagcc
      541 tgaaattatt gttaatatta agcaggctcc aaagagaacg ctcccgacta accatcctct
      601 agataattct gttagggctc cttctttaaa tagccctaga gaactaattg gacgcttctc
      661 tttaaatttc acccacataa ataacattac cgtactaatt gaagttgcta gtaaaatgat
      721 taactggacg atatcgctat cccactctag tttccaaaaa tcaagcgaga taatcaattc
      781 aataataata taggtaagga aatctgctgt aaatagtaat atcaaagcca ataatatagt
      841 taaaaggaca tgccttgata ctttaccttt tgcagtttcc tc
//
LOCUS       DBJYSH010000215          878 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.217, whole genome shotgun sequence.
ACCESSION   DBJYSH010000215 DBJYSH010000000
VERSION     DBJYSH010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..878
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.217"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
BASE COUNT          308 a          113 c          167 g          290 t
ORIGIN      
        1 aattatttat tttttcaaaa cagaataact atgtgaaaaa aaataatgtt aggtctctca
       61 aaaacacgta tagcaatttc gaatctcaaa tattttatta tgactattgt aatagttacg
      121 aatctattat tcaactcaac aataagaaaa ctcttcagta aatcattatg ttaaacaagc
      181 tctgatagta tggagataag tataggtaaa agtcgattta atctctgaaa caaaatttaa
      241 cttaattaat agaaccgttg acgtatcagg tatcaaatct ttttggttta tatgtgatat
      301 aatgaaagaa catattgaga tatttggcac cctcttgaaa ggcaaaggtt acttgtatga
      361 ttaaactaat agcaacagaa ataagaagaa gttaattgat acaatttaac gtaataaaat
      421 ccttgaaatc agcaaatttc aaggattttt tggtgatctt atggatagca ttgaggaaat
      481 aaaacttttt agaggagttg agtttggctc tcagcattgt aatcgttaaa tagtgtcaaa
      541 tcgttaaata atgtgatatt accaaatacg catcatgtgg aggtagtcgg actgctggtg
      601 agagagggat aaagcccacc gtttcagggt ttagaaaagc cttgatttca agcctttttt
      661 gaattttttg gtgaagtatc aatcaaacaa tctgaagtga ggtagttgga ttgatagcgg
      721 taaagtataa aatttattcc cacatacgca gagggggtgc aaaatgttct gacaggtagg
      781 gtgtctgtgg gaatagtttt gcccccactt ttttgatttt ctgatatcac caagatggtc
      841 gatagaagtg tgagttctat atgttgatac tacatatt
//
LOCUS       DBJYSH010000216          823 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.219, whole genome shotgun sequence.
ACCESSION   DBJYSH010000216 DBJYSH010000000
VERSION     DBJYSH010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..823
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.219"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            15..>823
                     /locus_tag="ACUBV3_002019"
     CDS             15..>823
                     /locus_tag="ACUBV3_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family putative permease subunit"
                     /protein_id="HHU3848485.1"
                     /translation="MIRFLILAGYFELSMYLKLSGKLNQYINTHYTYLAYISMVLSFI
                     LAIVQLIIWVKNMKMHSHLHGKIAKSTSPMILVFPVLVGLLVPTVSLDSTTVSAKGYN
                     FPLAAGSTGTVSQDGTRVQYLKPDTSTYFTSSAYEKEMQKELKKYKGSGTLTITTENY
                     MEVMELIYLYPEQFMDRQIQYTGFVYNEPKHEGYQFIFRFGIIHCIADSGVYGLLTTG
                     NQKSYPDNTWVTVRGTIKSEYNQLLQQNLPVLHIEESRQVSKPNNPYVYRV"
BASE COUNT          272 a          146 c          132 g          273 t
ORIGIN      
        1 gtgggggtaa tataatgatt cgatttctta ttcttgctgg ttattttgaa ttaagcatgt
       61 atttaaagtt atcaggtaaa ctaaatcagt atattaatac tcattatacc tatttggcct
      121 atatttcaat ggtcctatct tttattttag caattgtaca actaattatt tgggttaaaa
      181 atatgaaaat gcatagtcac ctacatggta aaatagctaa atcaacaagc ccaatgattt
      241 tagtatttcc cgttttagta ggtttgttag tacctacagt tagtttagac tcgaccacag
      301 tctctgccaa gggatataat ttccctctag cagccggctc tactggtact gtcagtcaag
      361 atgggacacg cgttcaatat ttgaaaccag ataccagcac ttattttact tcttcagcct
      421 atgaaaaaga aatgcaaaaa gagcttaaaa aatataaagg ctctggtaca ttaaccatca
      481 caactgaaaa ctacatggaa gttatggaac taatttatct ctatcccgag caattcatgg
      541 atcgccaaat acaatatact gggtttgttt ataacgaacc caaacacgaa ggatatcaat
      601 ttattttccg ctttggaatc attcattgta ttgctgattc aggagtttat ggtctactaa
      661 caactggaaa tcaaaaaagt tatccggaca atacttgggt aactgttaga ggtacaatta
      721 aatccgagta taatcaattg ttacaacaaa acttaccagt cttacacatt gaagagtctc
      781 gtcaagtttc taaacctaat aatccttatg tttatcgtgt ctt
//
LOCUS       DBJYSH010000217          801 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.220, whole genome shotgun sequence.
ACCESSION   DBJYSH010000217 DBJYSH010000000
VERSION     DBJYSH010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..801
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.220"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            complement(1..243)
                     /locus_tag="ACUBV3_002020"
                     /pseudo
     CDS             complement(1..243)
                     /locus_tag="ACUBV3_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000692244.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParA"
     gene            279..801
                     /locus_tag="ACUBV3_002021"
                     /pseudo
     CDS             279..801
                     /locus_tag="ACUBV3_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612023.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          294 a          143 c          127 g          237 t
ORIGIN      
        1 tgagcggtta aataactctt tatgaggaat cattgcaaga actgatttat cattctttag
       61 tgatcttatt aattcttttg aagagttggt attatgcttt atcatattac ctaaaaagaa
      121 taattttgct gtgacataac ttgctcgagt tttaaagtca aatgcctctg ttttaaattc
      181 ttcaagtctt tcagaaagat ggatgtatac tataaaagta gacaaaactt tgtgtgttac
      241 aatctgtttt aaaagacaca aaaacgaaag gacaacctat gtcaacattt aaacgctatg
      301 atgaagaatt caaacaatct cttgttaacc tttatcaaac tggaaaaact cagtctgaac
      361 tctgtaaaga ctatggggta tccacttcta cgcttgcaaa atggatcaaa cagtattccc
      421 aagtcaaact cgaagataat tctgtactta ctgccaagca aatacaagag ttacaaaaac
      481 gtaatgcgca gcttgaagag gagaacctta tcctaaaaaa agcaagtgcc atattcatgc
      541 aaaacttaaa gtaagactcc tcgctgtcta tcggttacgc tttgaacacg ccacaacaac
      601 cttgtgtcgt gttttacgtg tcaatcgctc cacttactat aaatttctaa aacataagcc
      661 ctcaaaaaga gaattggata atcaaattta tagaaaacaa atacttgaga tttataccaa
      721 agcgaacaaa agacttggtg tgaagtctat caaggtcatt cttcaaagag actatgacac
      781 aaaaatctct gaaggaagaa t
//
LOCUS       DBJYSH010000218          767 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.221, whole genome shotgun sequence.
ACCESSION   DBJYSH010000218 DBJYSH010000000
VERSION     DBJYSH010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..767
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.221"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            6..758
                     /locus_tag="ACUBV3_002022"
     CDS             6..758
                     /locus_tag="ACUBV3_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000482192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HHU3848486.1"
                     /translation="MFNKIGFRTWKSGKLWLYMGVLGSTIILGSSPVSAMDSVGNQSQ
                     GNVLERRQRDAENKSQGNVLERRQRDAENKSQGNVLERRQRDVENKSQGNVLERRQRD
                     AENKSQGNVLERRQRDVENKSQGNVLERRQRDAENKSQGNVLERRQRDADNKSQVGQL
                     IGKNPLFSKPTVSRENNHSSQGDSNKQSFSKKVSQVTNVANRPMLTNNSRTISVINKL
                     PKTGGDQNVIFKLVGFGLILLTSRCGLRRNEN"
BASE COUNT          263 a          123 c          176 g          205 t
ORIGIN      
        1 attaattgtt caataaaata ggttttagaa cttggaaatc aggaaagctt tggctttata
       61 tgggagtgct aggatcaact attattttag gatcaagtcc tgtatctgct atggatagtg
      121 ttggaaatca aagtcaaggt aatgttttag agcgtcgcca acgtgatgcg gaaaacaaaa
      181 gtcagggtaa tgttttagag cgtcgccaac gtgatgcgga aaacaagagc caaggcaatg
      241 ttttagagcg tcgtcaacgc gatgttgaga acaagagcca aggtaatgtt ctagagcgtc
      301 gccaacgtga tgcggaaaac aagagccaag gcaatgtttt agagcgtcgt caacgcgatg
      361 ttgagaataa gagccaaggc aatgttttag agcgtcgtca acgtgatgcg gaaaacaaaa
      421 gtcagggcaa tgttctagag cgccgccaac gtgatgcgga taacaagagc caagtaggtc
      481 aacttatagg gaaaaatcca cttttttcaa agccaactgt atctagagaa aataatcact
      541 ctagtcaagg tgactctaac aaacagtcat tctctaaaaa agtatctcag gttactaatg
      601 tagctaatag accgatgtta actaataatt ctagaacaat ttcagtgata aataaattac
      661 ctaaaacagg tggtgatcaa aatgtcattt ttaaacttgt aggttttggt ttaattttgt
      721 taacaagtcg ctgcggtttg agacgcaatg aaaattaagt ataatca
//
LOCUS       DBJYSH010000219          746 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.223, whole genome shotgun sequence.
ACCESSION   DBJYSH010000219 DBJYSH010000000
VERSION     DBJYSH010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..746
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.223"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            5..691
                     /locus_tag="ACUBV3_002023"
     CDS             5..691
                     /locus_tag="ACUBV3_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwaF family protein"
                     /protein_id="HHU3848487.1"
                     /translation="MIEFFSNIRTEIPQMPLLIHSLILSVLPFLMWLTLVNRDKPLYK
                     TIWSILLGLQLITIYTWFFWAKLPLSESLPLYHCRIGMFVVLLARPRILKDYFALLGV
                     VGGVLAMIHPDFYPYQFLHVTNIFFFIGHFALFVLSLLHLMTQSNLDKLNPKLIIQLT
                     LLINMSLIFINLLTGGNYGFMMKTPILGITNPFLNLLIVTTLLSFLVLFVKQIFQKVR
                     MRASFKTEES"
BASE COUNT          224 a          118 c          116 g          288 t
ORIGIN      
        1 taaaatgata gaattctttt ctaatatcag aacagagatt ccgcagatgc ctttacttat
       61 ccatagtttg attttatctg tcttaccttt tctgatgtgg ctgactttgg ttaatagaga
      121 taagcctttg tataaaacta tttggagtat ccttttagga cttcagttaa ttacgattta
      181 tacttggttt ttctgggcaa aattgccttt atctgaaagt cttccccttt accattgtcg
      241 aataggcatg tttgtcgttc tcttagcgag gcctagaata ttaaaggatt atttcgcatt
      301 gttaggagta gtaggtggag tattggcaat gattcaccca gacttttacc cttaccagtt
      361 tctacatgtg actaatattt tcttctttat tggacatttt gctttatttg tccttagttt
      421 actacatctg atgacacagt ccaatttgga caaattaaat ccaaaattga ttatacaact
      481 aacgcttctc attaatatgt ctttgatttt tattaacctc ttgactgggg gaaattatgg
      541 ctttatgatg aagacaccaa tactaggaat aacaaatccc tttttaaacc ttttgatagt
      601 gacaactcta ttaagttttc tcgtcctatt tgttaaacaa attttccaaa aagtgagaat
      661 gagagctagt tttaaaacag aagaatctta attattttaa tattattata ttatctagct
      721 attaaaaaat aaatagctgg ataatg
//
LOCUS       DBJYSH010000220          738 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.224, whole genome shotgun sequence.
ACCESSION   DBJYSH010000220 DBJYSH010000000
VERSION     DBJYSH010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..738
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.224"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            complement(317..400)
                     /locus_tag="ACUBV3_002024"
     tRNA            complement(317..400)
                     /locus_tag="ACUBV3_002024"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(364..366),aa:Leu,seq:caa)
     gene            complement(411..482)
                     /locus_tag="ACUBV3_002025"
     tRNA            complement(411..482)
                     /locus_tag="ACUBV3_002025"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(448..450),aa:Gln,seq:ttg)
     gene            complement(489..561)
                     /locus_tag="ACUBV3_002026"
     tRNA            complement(489..561)
                     /locus_tag="ACUBV3_002026"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(526..528),aa:His,seq:gtg)
     gene            complement(576..646)
                     /locus_tag="ACUBV3_002027"
     tRNA            complement(576..646)
                     /locus_tag="ACUBV3_002027"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(612..614),aa:Trp,seq:cca)
     gene            complement(653..733)
                     /locus_tag="ACUBV3_002028"
     tRNA            complement(653..733)
                     /locus_tag="ACUBV3_002028"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(697..699),aa:Tyr,seq:gta)
BASE COUNT          224 a          171 c          132 g          211 t
ORIGIN      
        1 attttcacga ttttaatttt ttagtcgtaa catatacact gaaaaatctt attattttat
       61 actacctatc tatcattcac aaacactttt attacttcag aacctatgac atttaggagt
      121 cctctttgaa tttcatttaa aatgttgagt ctccactaac tcttgaaaaa tttccttatt
      181 atttctgctt gttttaaaac ttcaataaca attgcaataa tgaaaaacaa atataagtaa
      241 ttttcagtaa ctttttctca aaattaccag tacaatacaa aaaagacaag gtctagaaac
      301 cttatctttt aaagtatacc ggcggccggg gtcgaaccgg cacgtccgtg aggacactgg
      361 attttgagtc cagcgcgtct gccaattccg ccacgccggc tatcttaaaa ctggggtagc
      421 tggattcgaa ccaacgcatg agggagtcaa agtcccttgc cttaccgctt ggctataccc
      481 catgaaaagg cgagtgatgg gaatcgaacc cacgaatgtc agagccacaa tctgatgtgt
      541 taaccacttc accacacccg ccatattaga aaaaacacgg gcagtaggaa tcgaacccac
      601 actgaaggtt ttggagacct tagttctacc tttaaactat gcccgtttac tatggagaga
      661 gagggattcg aacccccgaa cccgaaggag cggatttaca gtccgccgcg tttagcctct
      721 tcgctatctc tcctaagg
//
LOCUS       DBJYSH010000221          677 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.229, whole genome shotgun sequence.
ACCESSION   DBJYSH010000221 DBJYSH010000000
VERSION     DBJYSH010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..677
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.229"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            76..677
                     /locus_tag="ACUBV3_002029"
                     /pseudo
     CDS             76..677
                     /locus_tag="ACUBV3_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612023.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          255 a          120 c          100 g          202 t
ORIGIN      
        1 atctcatggt tatgcgagta tggatgtata ctataaaaat agacaacact ttgtgtgtta
       61 taacctgttt taaaagacac aaaaatctct gaaggaagaa tttaccgtct gatgaaggat
      121 atggcgctcc ctaaaatggc taccgttaag acaaaaacag ctcttaaaaa gactcaaaaa
      181 acgtatcctc aaaacttact caaccagaaa tttaatcctg ataaacctaa tcaagtatgg
      241 tctactgact tcacctatat ttctattgga tataagaaat atgtctatct ctgcgcaata
      301 attgatctct attctagaaa atatattgct tggaaactga gtcatcgtat ggatgcaaag
      361 ttagcatgtg acactctaga attagctctt aataaaagaa agattgaagg aacacttctc
      421 tttcattccg accaagggtc acaatttaag gccagggaat ttagaaaaat aattgatgac
      481 aacaatatca tgcattcttt ttctaaacct agatatcctt atgataatgc cgtaactgaa
      541 gcatttttca agtatttaaa gcatagacaa atcaaccaaa aaaattatca aaatatcaaa
      601 caggttcaat tagactgctt tgaatacatt gagaattttt ataacaatta catcccacat
      661 acggctaatc taggact
//
LOCUS       DBJYSH010000222          677 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.230, whole genome shotgun sequence.
ACCESSION   DBJYSH010000222 DBJYSH010000000
VERSION     DBJYSH010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..677
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.230"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            complement(<1..675)
                     /locus_tag="ACUBV3_002030"
     CDS             complement(<1..675)
                     /locus_tag="ACUBV3_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-like surface protein"
                     /protein_id="HHU3848488.1"
                     /translation="MFRRSKNNSYDTSQTKQRFSIKKFKVGAASVLIGLSFLGGVTQG
                     NLNIFEESIVAASTIPGSAATLNTSITKNIQNGNAYIDLYDVKLGKIDPLQLIVLEQG
                     FTAKYVFRQGTKYYGDVSQLQSTGRASLTYNIFGEDGLPHVKTDGQIDIVSVALTIYD
                     STTLRDKIEEVRTNANDPKWTEESRTEVLTGLDTIKTDIDNNPKTQTDIDSKIVEVNE
                     LEKLLVL"
BASE COUNT          210 a          140 c           82 g          245 t
ORIGIN      
        1 caatactaac aatttctcta attcattaac ctcaacaatt ttactatcaa tatctgtttg
       61 cgtcttagga ttattatcaa tatctgtctt aattgtatct aatcctgtta aaacctcagt
      121 acgactttct tccgtccact taggatcgtt tgcattcgtt ctaacttctt caatcttatc
      181 cctcaaggtt gttgaatcat aaatagttaa agcaacacta actatatcaa tttgtccatc
      241 agtctttaca tgtggtagtc catcttcacc aaatatatta taggtaagac tagcccttcc
      301 tgtactctgc aactgagaaa catccccata gtatttagta ccttgtctaa aaacatactt
      361 tgctgtaaaa ccttgttcta aaacaattaa ttgtaatgga tctattttac ctaattttac
      421 atcatataaa tctatgtaag catttccgtt ttgtatattt ttagtgatgc ttgtatttaa
      481 ggtcgctgca ctccctggaa ttgtagatgc agcaactatt gactcttcaa aaatattaag
      541 attaccttgt gtaaccccac ccaaaaaact aagaccaatt agtacagaag ctgcaccaac
      601 cttgaacttc ttaattgaaa accgttgttt cgtctgtgaa gtatcataac tgttattttt
      661 agaccttcta aacataa
//
LOCUS       DBJYSH010000223          666 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.231, whole genome shotgun sequence.
ACCESSION   DBJYSH010000223 DBJYSH010000000
VERSION     DBJYSH010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..666
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.231"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            <1..633
                     /locus_tag="ACUBV3_002031"
     CDS             <1..633
                     /locus_tag="ACUBV3_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0236 family transposase-like protein"
                     /protein_id="HHU3848489.1"
                     /translation="LITNSDMGKGYTSRVFKELGKALKVKKHEHFWDIYHVKEKLSSY
                     LRKYPIELTDFALDAVKKYNSDKLELVFDTVESLICDELEDQEFQKFKKKVLNNFKYI
                     KPAHLRNLSNRGIGIMESQHRKITYRMKRRGMYWSKWGISTMANMIILERANGLRELF
                     FGSWRKVYSEYKEGSFSAGRLFKKTDELDKFSKPLLKNGRKWSITGIKTK"
BASE COUNT          258 a           80 c          126 g          202 t
ORIGIN      
        1 ttaatcacta actctgatat gggtaaaggc tatactagta gagtttttaa ggaattagga
       61 aaagcactta aggtaaagaa acatgagcat ttttgggata tctatcatgt taaagaaaag
      121 ttaagttcat accttagaaa atatccaatt gaattaaccg attttgcttt agatgcggta
      181 aaaaaatata attctgataa gcttgaatta gtttttgata ctgttgaatc actgatttgt
      241 gatgaacttg aagatcaaga atttcagaag tttaagaaaa aagtattaaa taatttcaaa
      301 tatataaaac cagctcatct tagaaatctt tcaaatcgtg gtattggtat catggaatca
      361 caacacagaa agataacgta tagaatgaag cgacgtggca tgtattggtc aaagtgggga
      421 atctccacaa tggcaaatat gattatactt gaaagagcta acggtttacg agaattattt
      481 ttcggttctt ggagaaaggt atacagtgag tataaagaag gttcatttag tgcagggcga
      541 ctttttaaaa agacagatga attagataaa ttttctaagc cccttctaaa aaatggcaga
      601 aaatggagta taacaggaat caaaacaaaa tagaaaaagt ctaaagatac ctttatttct
      661 agcgtt
//
LOCUS       DBJYSH010000224          597 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.237, whole genome shotgun sequence.
ACCESSION   DBJYSH010000224 DBJYSH010000000
VERSION     DBJYSH010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..597
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.237"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            complement(45..563)
                     /locus_tag="ACUBV3_002032"
     CDS             complement(45..563)
                     /locus_tag="ACUBV3_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HHU3848490.1"
                     /translation="MRTYENKEELKKEISKAFEKYISEFNNIPETLKDKRVDEVDRTP
                     AENLAYQVGWTTLILKWEEDERNGLQVNTPSDNFKWNQLGDLYQWFTDTYAQLSLQEL
                     KAKLNKNINSIYAMIDALSQEELFKPHMREWADQATKTATWEVYKFIHVNTVAPFGTF
                     RTKIRKWKKVVL"
BASE COUNT          168 a          114 c           76 g          239 t
ORIGIN      
        1 ctctagtaaa caattttatg aaatttttat tttacaagct acaattataa tactaccttc
       61 ttccattttc tgatttttgt tctgaaagtt ccaaaaggtg caactgtatt aacatgaata
      121 aacttatata cttcccaagt cgctgtttta gtcgcctgat cagcccattc tctcatatgt
      181 ggtttaaata attcttcctg acttagtgca tcaatcatgg cataaataga attaatgttt
      241 ttatttagtt tggcttttag ctcttgtaga gataattgag cgtaagtatc tgtgaaccat
      301 tgatacaagt cgcctagttg attccattta aagttatctg atggcgtatt aacttgaagt
      361 ccatttcttt catcctcttc ccatttaaga atcaaagttg tccatccaac ctgataagca
      421 agattctcag ctggagttct atcaacttca tcaactcttt tatcttttaa ggtttccgga
      481 atattattaa attctgaaat atatttctca aatgctttac ttatttcttt tttcagctct
      541 tctttatttt cgtatgttcg caaaaaaatc acctccattc tgatttatca gtaccct
//
LOCUS       DBJYSH010000225          555 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.240, whole genome shotgun sequence.
ACCESSION   DBJYSH010000225 DBJYSH010000000
VERSION     DBJYSH010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.240"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            <1..>555
                     /locus_tag="ACUBV3_002033"
     CDS             <1..>555
                     /locus_tag="ACUBV3_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000160532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element ISSag9 family transposase"
                     /protein_id="HHU3848491.1"
                     /translation="KTFYLAKQAQRNYEKLLVESREGIPLNKETFWKIDRVLSNGVKK
                     GQRIYHILKTNDLEVSSSTVYRHIKKGYLSITPIDLPRAVKFKKRRKSTLPPIPKAIK
                     EGRRYEDFIEHMNQSELNSWLEMDTVIGRIGGKVLLTFNVAFCNFIFAKLMDSKTAIE
                     TAKHIQVIKRTLYDNKRDFFELFPV"
BASE COUNT          187 a          124 c          107 g          137 t
ORIGIN      
        1 aagaccttct atcttgctaa gcaagctcaa agaaactatg aaaaactttt agttgaatct
       61 agagaaggaa tccctttgaa caaagagacc ttctggaaaa tagatagagt gctttctaat
      121 ggggtcaaga agggccaacg tatctatcat atcctcaaaa ccaacgatct agaagtgagt
      181 tcttcaaccg tttatcgaca catcaagaaa ggctacctat ccatcacacc aatcgaccta
      241 cccagagctg tgaagttcaa aaaaaggcga aagagcacac tccctcctat tccaaaagcg
      301 attaaagaag ggcgacggta cgaggatttt atagaacaca tgaaccaatc agagctgaac
      361 tcctggcttg aaatggacac ggttattgga cggattggtg gaaaggtcct tctcaccttc
      421 aatgtcgcct tctgtaactt catctttgcc aaactgatgg attctaagac agctatcgaa
      481 actgctaaac acatacaggt cattaagagg acactctatg ataacaagag agacttcttc
      541 gaactctttc ctgtt
//
LOCUS       DBJYSH010000226          544 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.241, whole genome shotgun sequence.
ACCESSION   DBJYSH010000226 DBJYSH010000000
VERSION     DBJYSH010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.241"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            <1..163
                     /locus_tag="ACUBV3_002034"
     CDS             <1..163
                     /locus_tag="ACUBV3_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HHU3848492.1"
                     /translation="KDKYDPTGKAQQVNGKGNKLPATGENATPFFNVAALTIISSVGL
                     LSVSKKKED"
BASE COUNT          190 a           72 c           86 g          196 t
ORIGIN      
        1 taaggataaa tatgatccaa caggtaaagc tcagcaagtc aacggtaaag gaaataaact
       61 accagcaaca ggtgagaatg caactccatt ctttaatgtt gcagctttga caattatatc
      121 atcagttggt ttattatctg tttctaagaa aaaagaggat taatcttttg acctaaaatg
      181 tcactaaatt tttcaccatt tattggtgtg aacacattaa taaagttatg catctctctc
      241 caacaaaatt aattaaagtg tttcaatttt tcgagattaa ttcttgaaaa aagcctatcg
      301 agattattaa tttcgatagg cttttgattt tgtgtaagcg tccaatatac cttgttattg
      361 gacgcttact ataaaactga agaaatgata ttatttagtt gaaatttgta aatgaagtgt
      421 atcattagag cctaagattt taatcctagg atctcttttg atgatttaaa atttttaggt
      481 gtaatcaaat gtaaggttag atgcaaaatt ttctaaaatg actgtttgtc ttttcaagta
      541 atta
//
LOCUS       DBJYSH010000227          541 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.242, whole genome shotgun sequence.
ACCESSION   DBJYSH010000227 DBJYSH010000000
VERSION     DBJYSH010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..541
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.242"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            complement(1..73)
                     /locus_tag="ACUBV3_002035"
     tRNA            complement(1..73)
                     /locus_tag="ACUBV3_002035"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(37..39),aa:Ile,seq:cat)
     gene            complement(93..166)
                     /locus_tag="ACUBV3_002036"
     tRNA            complement(93..166)
                     /locus_tag="ACUBV3_002036"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(130..132),aa:Met,seq:cat)
     gene            complement(182..255)
                     /locus_tag="ACUBV3_002037"
     tRNA            complement(182..255)
                     /locus_tag="ACUBV3_002037"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(219..221),aa:Pro,seq:tgg)
     gene            complement(263..336)
                     /locus_tag="ACUBV3_002038"
     tRNA            complement(263..336)
                     /locus_tag="ACUBV3_002038"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(300..302),aa:Arg,seq:acg)
     gene            complement(348..432)
                     /locus_tag="ACUBV3_002039"
     tRNA            complement(348..432)
                     /locus_tag="ACUBV3_002039"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(396..398),aa:Leu,seq:taa)
     gene            complement(441..512)
                     /locus_tag="ACUBV3_002040"
     tRNA            complement(441..512)
                     /locus_tag="ACUBV3_002040"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(478..480),aa:Gly,seq:gcc)
BASE COUNT          150 a          153 c          122 g          116 t
ORIGIN      
        1 ggaccttgta ggactcgaac ctacgaccgc tcggttatga gccgagtgct ctaaccagtt
       61 gagctaaagg tccaaagtct caataaaata aatagcggcg gaggggatcg aacccccgac
      121 ctcccgggta tgaaccggac gctctagcca gctgagctac accgccataa aaatatatcc
      181 atcgggaaga caggattcga acctgcgaca ccttggtccc aaaccaagta ctctaccaag
      241 ctgagctact tcccgaaaaa tatgcaccct agaggagtcg aacctctaac cgcctgattc
      301 gtagtcaggt actctatcca gttgagctaa gggtgctaaa tattatatgc cgaggaccgg
      361 aatcgaaccg gtacgatgtt taccatcgca ggattttaag tcctgtgcgt ctgccagttc
      421 cgccaccccg gcctctaaca agcgaacgac ggggttcgaa cccgcgaccc tcaccttggc
      481 aaggtgatgt tctaccactg aactacgttc gcactaaaga cactatttat cctataaaat
      541 t
//
LOCUS       DBJYSH010000228          513 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.245, whole genome shotgun sequence.
ACCESSION   DBJYSH010000228 DBJYSH010000000
VERSION     DBJYSH010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..513
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.245"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
BASE COUNT          165 a          108 c           67 g          173 t
ORIGIN      
        1 ttattacctt tggtaacacc aaaacataca cgaactttta ttcatatgtg ttaccatttt
       61 acatctttca tattgagata tttgattagc aatacctcct tgactaacaa aaattactaa
      121 cacatctagg ggtataccct agtttatatt tgttcatgat tacctcaagc gagcatgcgc
      181 taatggtaat gaactattac cacattgtct gtcttcctta aattatataa ggacttatgt
      241 gtcaccgtta ctgtagcgac ctacatctaa acccaacata agtgcccctc caactatttc
      301 taggaaatga agaaaacttc aaatccttgt gactgtttgc aacatcaatg cattgataac
      361 aattttagtg tatcgccatt cttatagcga cccatattaa aaattaacat aagcgctacc
      421 tccattaact aaagttagct tgcctaccaa tctttctttt ggagactagt ctccattctt
      481 atacctagag atattgtaat agatcatgga taa
//
LOCUS       DBJYSH010000229          489 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.247, whole genome shotgun sequence.
ACCESSION   DBJYSH010000229 DBJYSH010000000
VERSION     DBJYSH010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..489
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.247"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
BASE COUNT          185 a           79 c           84 g          141 t
ORIGIN      
        1 gatatgtata ccgtaactat attgcattga gttggttgtt tgcgatagca acgtcaagac
       61 agcaataaaa ttgaaaaaat caataagata aaaaggattt aaaaaattga ttattattgt
      121 ggaagggata agcttaatga tgattttaac agcatgtgac ttagtaaaaa aagatcacca
      181 atgggttcaa gaacattgaa aatacttaga agctggatca aaagttatca aagaagtcag
      241 ccaaaacatt caaccagtta tttctgatct gaatagctat ctcaatggag cgacaagtgc
      301 ttttgagcaa tcggattcga gtgcagctac tcatattaaa aataacacca tcgcccaact
      361 taaaaaaagc agtaccaaat cgtgtgagaa attaccaggc taattgtgag tgagaatcta
      421 ttgttctcac ttttttatta tcaaaacgct taaattcttt acttagacct cgcctacaca
      481 cttcagata
//
LOCUS       DBJYSH010000230          472 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.249, whole genome shotgun sequence.
ACCESSION   DBJYSH010000230 DBJYSH010000000
VERSION     DBJYSH010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.249"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            complement(<1..>472)
                     /locus_tag="ACUBV3_002041"
     CDS             complement(<1..>472)
                     /locus_tag="ACUBV3_002041"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HHU3848493.1"
                     /translation="RPLEESDYDSWYTSFMNRGPSLTPFDDGILDMSMCDINWFKNLV
                     NLQRKEWESDIVYIFGVFLKSGEHVGMLNIVTLARADMQWGELGYVFHNQFWSNGYAF
                     ESILALLNSTYEKLGFHHIEAQITPGNERSENLVRRLGLTYETTRKDFSFENGKW"
BASE COUNT          157 a          105 c           50 g          160 t
ORIGIN      
        1 ccattttcca ttctcaaatg aaaaatcttt tcgagttgtc tcataggtta atcccaatct
       61 tctaaccaaa ttttccgatc gttcattccc aggagtaatc tgtgcttcaa tatgatgaaa
      121 acctaatttt tcataagtac tattaagtaa tgccaagatt gactcaaatg catagccatt
      181 actccaaaat tgattatgaa aaacatatcc caattctccc cattgcatat ctgctcttgc
      241 aagggtaaca atatttaaca ttcctacgtg ctcaccgctt tttagaaaaa caccaaatat
      301 atatactatg tcactttccc attctttcct ttgtaaattt actaaatttt taaaccaatt
      361 aatgtcacac atagacatat caagtatccc atcatcaaat ggagttaaag atggtccacg
      421 attcataaaa ctagtatacc aactatcata atcactttct tctagtggcc tt
//
LOCUS       DBJYSH010000231          455 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.250, whole genome shotgun sequence.
ACCESSION   DBJYSH010000231 DBJYSH010000000
VERSION     DBJYSH010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..455
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.250"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            complement(17..394)
                     /gene="mscL"
                     /locus_tag="ACUBV3_002042"
     CDS             complement(17..394)
                     /gene="mscL"
                     /locus_tag="ACUBV3_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HHU3848494.1"
                     /translation="MIKELKEFLFKGNVLDLAVAVILGAAFNAIITSLVKDVITPLIL
                     NPVLKAAGVSNIAQLSWNGVAYGNFLSAVINFLIVGTTLFFIVKAANKVMAKKPAEEE
                     IIEVVEPTQEQLLAEIRDLLANK"
BASE COUNT          158 a           78 c           74 g          145 t
ORIGIN      
        1 taatccgagt cttttattat ttgttagcga gtaagtcgcg gatttcagca agtaattgct
       61 cttgtgttgg ttcaacaact tcaataattt cttcttcagc aggtttctta gccataacct
      121 tgttagcagc tttaacgatg aagaataaag tagtaccaac gattaagaaa ttaataactg
      181 cacttaagaa attaccgtaa gcaacaccat tccatgaaag ctgtgcgata tttgaaacgc
      241 cagcagcttt taatacagga ttaaggataa gcggtgtaat aacgtcttta acaagtgaag
      301 tgatgatagc attaaaagct gcacctaaaa taacagcaac agcaaggtct aaaacattac
      361 ccttaaataa aaattctttt agttctttaa tcatgtgaac aagctcctta atattattta
      421 attcacgttc attatagcag atttcttttg agaaa
//
LOCUS       DBJYSH010000232          442 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.251, whole genome shotgun sequence.
ACCESSION   DBJYSH010000232 DBJYSH010000000
VERSION     DBJYSH010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.251"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            <1..95
                     /locus_tag="ACUBV3_002043"
     CDS             <1..95
                     /locus_tag="ACUBV3_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HHU3848495.1"
                     /translation="EKTIAKGKELADDAKDAVEGAVDAVKEKLK"
     gene            137..325
                     /locus_tag="ACUBV3_002044"
     CDS             137..325
                     /locus_tag="ACUBV3_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Paratox"
                     /protein_id="HHU3848496.1"
                     /translation="MSIRTDIDEFKEAIDKGYISGNTVAIVRKNGKIFDYVLLHEEVR
                     EEEVVTVERVLDVLRKLS"
BASE COUNT          169 a           52 c           93 g          128 t
ORIGIN      
        1 tcgaaaaaac aattgctaaa ggcaaagaac tagcagatga tgctaaagat gctgttgaag
       61 gggcagtaga tgctgtcaaa gaaaaactga agtaacaaat aaccgctcat ttaacgagcg
      121 gttatttgtt atctttttgt ctatcagaac agatatagat gagtttaaag aagcgattga
      181 taaaggctat atttcaggga acacagtagc gatagtgcgt aaaaacggaa agatatttga
      241 ttatgtgtta ctacacgaag aagtgagaga agaagaggtt gttacagttg agagagtgct
      301 tgatgtactg aggaagttat cataataacg gaccaaaatt cgccccaaaa agtattttaa
      361 aatagaataa aataaaaaaa tatgcttttt attatggatg tatcgtgtat tttaaccttt
      421 aatatatcct attttaatgg ta
//
LOCUS       DBJYSH010000233          377 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.253, whole genome shotgun sequence.
ACCESSION   DBJYSH010000233 DBJYSH010000000
VERSION     DBJYSH010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..377
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.253"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
BASE COUNT          119 a           77 c           42 g          139 t
ORIGIN      
        1 ctatttttga tttcacaaaa aaaccttctt ctactttttt agactgattc tttcttccca
       61 cttcttcgta tcaatatcat atatttgaag aactaaactc tcagagtttc cagtcaattt
      121 tagtaataat cccaaaacta atccttccta caaaccaacg gataatttta tttcaactgg
      181 tcctggtttt aattttactt gtgcaatttc gcttgttata ccaactattg atatcgccga
      241 tactcctatt cctaatgatg cgcacgcatc attagttaaa caaagtactt taaatcctac
      301 agagggtcat tccatcaatt aatggcatag gttttggcac tagaaactga atatgaattc
      361 tattatctat taaaaaa
//
LOCUS       DBJYSH010000234          294 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.261, whole genome shotgun sequence.
ACCESSION   DBJYSH010000234 DBJYSH010000000
VERSION     DBJYSH010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..294
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.261"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            <1..>294
                     /locus_tag="ACUBV3_002045"
     CDS             <1..>294
                     /locus_tag="ACUBV3_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006740417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GA-like domain-containing protein"
                     /protein_id="HHU3848497.1"
                     /translation="KPDSQDAAEAAAEAAVKAAEDAAQAGKDKKAEVEADGVVNPDEK
                     SAVDGLNDVTTEKKGTATPLVDSLPEGPVKEALKARLDQVTTSEVTVNDADSNG"
BASE COUNT          103 a           66 c           78 g           47 t
ORIGIN      
        1 aaaccagatt cccaagatgc cgcagaagcc gcagctgaag cagccgtgaa agcagccgaa
       61 gatgccgccc aagcaggcaa agataagaaa gctgaagttg aagcagatgg tgttgttaat
      121 ccagatgaga aatcagccgt agatggcttg aacgatgtca caactgagaa gaaaggtaca
      181 gcaactccgt tagtcgatag cttaccagaa ggcccagtca aagaagcgtt gaaagcacgt
      241 cttgatcaag taacgacatc agaagtaacc gtcaacgatg cggatagcaa cggc
//
LOCUS       DBJYSH010000235          290 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.262, whole genome shotgun sequence.
ACCESSION   DBJYSH010000235 DBJYSH010000000
VERSION     DBJYSH010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..290
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.262"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            complement(54..135)
                     /locus_tag="ACUBV3_002046"
     tRNA            complement(54..135)
                     /locus_tag="ACUBV3_002046"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(99..101),aa:Leu,seq:tag)
     gene            complement(145..217)
                     /locus_tag="ACUBV3_002047"
     tRNA            complement(145..217)
                     /locus_tag="ACUBV3_002047"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(182..184),aa:Lys,seq:ttt)
     gene            complement(220..290)
                     /locus_tag="ACUBV3_002048"
     tRNA            complement(220..290)
                     /locus_tag="ACUBV3_002048"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(257..259),aa:Asp,seq:gtc)
BASE COUNT           77 a           87 c           66 g           60 t
ORIGIN      
        1 tctgattaca aatcagatgc tctaccaact gagctaagcc ggcaatctac taatgcgggt
       61 gaagggactt gaacccccac gccgttaagc gccagatcct aaatctggtg cgtctgccaa
      121 ttccgccaca cccgcatttc taaatgaccc gtactgggct cgaaccagtg acccattgat
      181 taaaagtcaa ttgctctacc aactgagcta acgagtctac ggtcccgacg ggaatcgaac
      241 ccgcgatctt cgccgtgaca gggcgacgtg ataaccgcta cactacggga
//
LOCUS       DBJYSH010000236          274 bp    DNA     linear   BCT 03-SEP-2025
DEFINITION  TPA_asm: Streptococcus agalactiae isolate GBSIR0703
            SAMD01592451-rid27500613.denovo.263, whole genome shotgun sequence.
ACCESSION   DBJYSH010000236 DBJYSH010000000
VERSION     DBJYSH010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01592451
            Sequence Read Archive: DRR702545
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002905999.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002905999.1
            Genome Coverage       :: 470x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2025 19:58:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,049
            CDSs (total)                      :: 2,007
            Genes (coding)                    :: 1,954
            CDSs (with protein)               :: 1,954
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 21 of 53
            Pseudo Genes (incomplete)         :: 25 of 53
            Pseudo Genes (internal stop)      :: 16 of 53
            Pseudo Genes (multiple problems)  :: 6 of 53
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..274
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01592451-rid27500613.denovo.263"
                     /isolate="GBSIR0703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            <1..195
                     /locus_tag="ACUBV3_002049"
     CDS             <1..195
                     /locus_tag="ACUBV3_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000160532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HHU3848498.1"
                     /translation="NLTQEDINLALSHINSVKRQALNGKTAYELFSFTYGKDIASILG
                     IEEITAEDVCQSPKLLKDKI"
BASE COUNT           99 a           63 c           43 g           69 t
ORIGIN      
        1 aacttgactc aagaggacat taatctggca ctatctcata tcaacagcgt taagagacag
       61 gctctaaatg gtaaaacggc ttatgagcta ttctctttta cttacggaaa agacatcgca
      121 agtatactcg gtattgagga aattactgcg gaagacgtct gccaatctcc caaactatta
      181 aaagacaaaa tctaacctaa tcaactaact gatacaacct aaacgtctca cactaacttc
      241 aaccatagcg gaacttaatc tgaaacgctt tcag
//
